LOCUS       DAOOYG010000001        59461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_420_length_59461_cov_6.629482, whole genome shotgun sequence.
ACCESSION   DAOOYG010000001 DAOOYG010000000
VERSION     DAOOYG010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 59461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59461
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_420_length_59461_cov_6.629482"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PK288_00005"
     CDS             <1..598
                     /locus_tag="PK288_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPM17329.1"
                     /translation="EAMQERQITIGESTYHLEEPFLVLATQNPIEQEGTYPLPEAQVD
                     RFMMKVLIDYPSIEEEKLIIRENLGKEFPRPDTVLKPEDIIRARDIVKEVYMDEKIEN
                     YILNIVFASRNPGKYGLPSYNSMIAYGASPRASINLALASKAYAFIRRRGYVIPEDVR
                     TICADVMRHRIGLTYEAEAENINQDKIISEILNVVEVP"
     gene            673..1536
                     /locus_tag="PK288_00010"
     CDS             673..1536
                     /locus_tag="PK288_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPM17330.1"
                     /translation="MEAIELLKKVRRIEIKTRGLTRQIFAGEYHSAFKGRGIAFNEVR
                     EYQYGDDIRNIDWNVTARFNHPYVKVFEEERELTVMLLIDLSGSGNFGTKVSFKRDLM
                     TEVAAVLSFSAIYNNDKIGVIFFSETVEKFIPPQKGRKHILRIIRELLDFKPRSNNTN
                     LGEPLRFLTNAIKKRCTAFIISDFIAPDFDEALKIAANKHDVVALRVYDPFERELPDI
                     GFVKVRDSETGTEKWIDTSSSLTRKAYSRWWSDHTGNVEGTFRKYGVDSVSLSTGEDY
                     VKPLIKLFENR"
     gene            1590..2528
                     /locus_tag="PK288_00015"
     CDS             1590..2528
                     /locus_tag="PK288_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17331.1"
                     /translation="MANLNCQDITVITKFDTSRIYIGDQINFSVTVEQPARLQIPFPV
                     FRDTLVKNIEILAGPVVDTTIVADSLIRVTGQYLVTSFDSGYYRVNPVYIELRDAGGV
                     KRYYSDYSVLEVARVKATPPDTVKGIFDIVKPYRAPVTLGEIMPWILLGIVAVAAIWF
                     IIRFGRKLIKPKASLPEVIPVEAAHVIALRELERLKDEKLWQSGETKKYYTRLTEILR
                     KYLENRFGVFSLEMTTSETLDALIRTGFKKDDNYNLLKSVLNGADLVKFARYKPEASE
                     NEIHYENAWNFVLATRKEEVSESPASGNNLNKDTST"
     gene            2534..3517
                     /locus_tag="PK288_00020"
     CDS             2534..3517
                     /locus_tag="PK288_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM17332.1"
                     /translation="MIFADPNFLYLLLLVPVIIAFYVLKQHNASASLSMPELQPFNQA
                     GKTFRNYLRHALFAFRVLAFVFLILVLARPQATNTFQDMSTEGIDIMLTMDISGSMLA
                     RDFKPDRLEASKNVATEFISGRPYDRIGLVVFSGESFTQCPLTTDHAVLINLMREIKS
                     GMIEDGTAIGMGLANAINRIKDSEAKSKVIILLTDGVNNRGEIAPATAAEIAKTYGIR
                     VYTIGVGTQGMAPYPVQTPFGTRYQNMPVEIDVAILTQIARETGGRYFRATDNNKLVE
                     VYNEIDKLEKSIIDVRQFSKKEEKFLWPAFAAFCLMALELILHYTLLRNLT"
     gene            3536..4570
                     /locus_tag="PK288_00025"
     CDS             3536..4570
                     /locus_tag="PK288_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPM17333.1"
                     /translation="MFRFANPQYLYLLLILPVMLVIFLLNNLRRKRLIRKIGTPALVS
                     KLIPEMSGNRPLIKFLIMMLAVSSAVIMLARPQYGSKLEEVKKQGVEVIIALDVSNSM
                     LAEDIQPNRLVRAKQAISRLVDNLSNDRIGLIVFAGDAYTQVPVTTDYVSAKMFLSTI
                     TPDMVAKQGTAIGAAIELGIRSFSPGEGKSRAMIIITDGENHEKDPLSSAEEAAKAGI
                     IIHTIGIGSPEGVPIPISSGGRKDYLKDTEGNTVITKLDEETLKNIALRTGGSYVRAS
                     NSSLGLDEIFSNIRKMKKEELEGTIYTEYNDQFRIFAGISAIFLILEFLIMNRKNRRL
                     ANVRLFKFKI"
     gene            4581..5366
                     /locus_tag="PK288_00030"
     CDS             4581..5366
                     /locus_tag="PK288_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM17334.1"
                     /translation="MHILRHKYLISDHGIVLTLCILYLFPATLTGQAEKKFIRQGNRE
                     YEKEKFAESEISYRRALDKNKAFPDAVFNIGDALYKQEKYDDAVRQFEGSSAMTDNPD
                     KKSEAFYNQGNSLLMGGKLQESIEAYKNSLRLKPGNLEAKYNLAFAQDLLRKQEEQKN
                     QDKNKQDNREQNQERQNNQDKENNDKDQEKQQEQNNQQQQQQQQQQQQQQQQQQQQQE
                     QQGISREDAERLLNSIANDEKNVQEKVKRAKVADTRVRTLKNW"
     gene            5373..7193
                     /locus_tag="PK288_00035"
     CDS             5373..7193
                     /locus_tag="PK288_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPM17335.1"
                     /translation="MKLHRLILSLLLIVLRLNSQGQDITVAADYPGVVRTGEQFTITW
                     TINSGGGEFVPPSFDGFYKLMGPQTSYSSSTQIINGKITRETTYSYSYYLQAVNSGNF
                     VLAPATFIYKNRTYSSDSIRIEVTGTGSNQQGRTMPGASGNEITEPVRETKDDIFLNL
                     SVNKKDVYIGEHIVATVKIYTKVDISGISEIKYPGFEGFLRTDLNTPPLTSLQRENVN
                     GTIYGTGIIQQFLIYPQVSGEITIDPVQIKVLIQQKSGRSDPFFGDFFATYTTVPRVV
                     VSPAVKIKSRPLPETKPDDFSGIVGKISLAASLDKDSVKVNDAVNLKIILSGTGNLRL
                     ANAPDLKLPPDIEVYDPKISDDLKNTTEGTSGRKIFEYLLIPRHHGEYSIPPVSFSYF
                     DVSTKKYEKLTVPGLHFYASRSDQPGTGITVFGGVSKEDVKYLGRDIRFIDQNPGSLN
                     KPMNILTSKRSFYTAYAGAFILFLAILFVRREHVRRNSDIAMVRNRKAAKVAGKRLKT
                     AKACLKEGPSDTFYDEILKAIWGYLSDKLNIPVSDLTRNRAVESLRERGIAEEQIDKL
                     VSVLDACEYARYAPSSSGTEAARLYEDASDFISYVENPVR"
     gene            7195..7965
                     /locus_tag="PK288_00040"
     CDS             7195..7965
                     /locus_tag="PK288_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPM17336.1"
                     /translation="MKHIKIILFLLIFFLPTARPAFAQELPAEKFSRGVEYYKSANFK
                     EALDVWMDIYNTGYRSAALYYNIGNACFKLNNVPGAILFYERAKLLKPGDDNIEYNLQ
                     IARTMVVDKTEEIPGLFFVKWFDFISLLVTTNNWAMISLAAFIFALISSSIYLYSSSY
                     RLKIISFWLALALFVLSACTLSFSFRNRSLVHNSRSAIIFSPSVNGKSSPDASGTDLF
                     ILHEGSKVTIVDEVGQWYEIRLSDGNKGWVPSDCLSKI"
     gene            complement(8094..8759)
                     /gene="ung"
                     /locus_tag="PK288_00045"
     CDS             complement(8094..8759)
                     /gene="ung"
                     /locus_tag="PK288_00045"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPM17337.1"
                     /translation="MDVKIEEGWKLRLNDEFRKEYFERLTRFVREEYRNTVVYPPGSL
                     IFNAFNLCPFDRVKVVILGQDPYHGPGQAHGLCFSVRDGIEFPPSLVNIFKEIGSDLG
                     IDPLPSGNLERWASQGVLLLNATLTVRAHAAGSHQKKGWEEFTDSVIKVLNDERKNLV
                     FFLWGAYAQKKGESIDYQKHLVLKSVHPSPLSAQRGFFGNRHFSKCNEYLKMNGITPI
                     NWA"
     gene            9038..9484
                     /locus_tag="PK288_00050"
     CDS             9038..9484
                     /locus_tag="PK288_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPM17338.1"
                     /translation="MGKEIERKFLVKPGFRASLPEAVNIVQGYFSVDPLRTVRLRIFN
                     EKALLTVKSMAGSMNLARYEWEIEVPIAMAREMMDICLPQRITKSRYSIPWGNHTFEV
                     DVFHGKHEGLVIAEIELDSEGEQFEKPDWLGDEVTGNPDYYNSNLV"
     gene            complement(9490..9858)
                     /locus_tag="PK288_00055"
     CDS             complement(9490..9858)
                     /locus_tag="PK288_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPM17339.1"
                     /translation="MRGLNTILLLVLSNAFMTVAWYGHLRFKTLPGFTNLSLLAVILL
                     SWFIALFEYSLQVPANRIGFEGNGGPFSLVELKVIQEVISILIFTSFTLVVFKSEHFR
                     LNHLIAFVFLILAVYFVFKK"
     gene            complement(9906..10385)
                     /locus_tag="PK288_00060"
     CDS             complement(9906..10385)
                     /locus_tag="PK288_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM17340.1"
                     /translation="MITLSGGIERQELKAIEEILRSTDFFYEFEIQTALEIASETLTH
                     GTEKSGYYWMKIKTEDGLKAFANYGKNPFSTHSWDLYWIAVHGDQRNNHYGTSLLKAV
                     EEDVKAKGGRILWIETSGRPLYASTEAFYRKNGYELQATLKDFYAPGDPKRIYSKTL"
     gene            complement(10392..11363)
                     /locus_tag="PK288_00065"
     CDS             complement(10392..11363)
                     /locus_tag="PK288_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPM17341.1"
                     /translation="MKKKVCCILYNQPGNNALPDETDVLNQVEYVAENLGKLGMKSYR
                     KGITDDFMREIPLLAGERPDFVFNLVESINNKGELCYFIPALLNMYSIPYTGNSLEAL
                     FVTTSKALSNQIMVREGIKIPATYYPSQFSKLEPGKSYIIKPVWEDGSLGITKDSVFT
                     YTPGYERNFEGKNDSHWIIQEYIDGREFNISILSGMNGPEVMPPAEMIFHNFDHGMPK
                     IVDFRAKWIEESFEYKNTVREFPHSRLDPMLREKIDETALKCWNVFGLRGYARVDLRV
                     DQAGNPLVIEVNANPCISPDSGLVAATIEAGIPMTEVISRIISDLNY"
     gene            complement(11451..12440)
                     /locus_tag="PK288_00070"
     CDS             complement(11451..12440)
                     /locus_tag="PK288_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17342.1"
                     /translation="MRVGITYDLRSWYIDRGYSMEDTAEFDKQETIDAIDNALVRMGF
                     ETEQIGNCFQLMSALSSDKRWDLVFNIAEGLYGDGRESVVPALLDQYRIPYVFSGPVV
                     LGVSLNKYLSRLIVSAAGVPVSPGMLVSCLDDIKKCNLQFPLFIKPVSEGTGKGITEK
                     SLVRSWKELRERAGFVLSRFNQPALVEEYLPGKEFTVGVIGSGSKAVAIGGMEIECRD
                     NLPYSVETKENYQIFCKYTPLEGVYEEECKTLALSAWNAIGGVDGGRVDLRVDVNGKM
                     CFIEVNPLAGLHPVHSDLPILANMNGINFQSLIEMIMRSALERNNLKLPAPIT"
     gene            complement(12451..13692)
                     /locus_tag="PK288_00075"
     CDS             complement(12451..13692)
                     /locus_tag="PK288_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPM17343.1"
                     /translation="MNANNNEVGALLGGGRLVGCSSDEEPGAKTMIFSQNVIPNQLVS
                     NRSSEFMNRYFPLTTLESWNDWKWQLRNAFTSLEDLRKIMKLTQKEILAINNLKGRLP
                     LRITPYFASLIYRSKPTHPLRRNVVPVVEELLEGRMEQSDPLHERAYSPVKGIVHRYP
                     DRVLFTVTQVCSNYCRYCTRSHSVGRLDKLGRQDFEKAFSYIASHREVRDVLISGGDP
                     LTMSDELLDYILHNIRKIEHVEIIRIGTRVPVVLPQRITDKLVSILKKYHPLYISLHF
                     SHPDEITEECANACIKLADNGFPLGSQTVLLKGINDSVPVMKELMHRLLKIRVRPYYL
                     YQCDLIPGSGHFRTTVKKGLEIIAGLRGYTSGYAVPTFVIDAPGGGGKIPLLPDYVVE
                     HNSERIVLRNYKGDLCEYPEK"
     gene            complement(14273..14746)
                     /gene="arfB"
                     /locus_tag="PK288_00080"
     CDS             complement(14273..14746)
                     /gene="arfB"
                     /locus_tag="PK288_00080"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HPM17344.1"
                     /translation="MRLAQAVICLKFLNEVTPDELKKRIPASELEMITSRSSGPGGQN
                     VNKVNTKVEIRFNVKDSPFLSDAEKDLIFSRLKNRISASGIISVRSQSGRSQARNREL
                     ASERLFAILAESLSTEPERIPSRPTRSSVEKRLGEKRKRADLKKTRKTSRKVHDN"
     gene            complement(14703..17438)
                     /locus_tag="PK288_00085"
     CDS             complement(14703..17438)
                     /locus_tag="PK288_00085"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPM17345.1"
                     /translation="MKSILASLFPVLFSLAAYSQRDLTVYDLSCEYKKDPVGVDALHP
                     RLSWKISSTGFNVMQTAYSIRVATDRKFSGSGMTWQTGKINSGESVFIPYEGNELKPG
                     QKYFWQVKVWDNKGRESKWSEAGSWETGLLSPAGWKAKWIELEADTNRYSPSPHFRKE
                     FSVDRSVKSARIYVTSHGFYELHLNGKKVGDQVLTPGWTSYLKRLQYQVYDVTAMLVP
                     GKNAIGALLGDGWYRGTLAWGNNWAIYGKHLGLLMQLHIRYSDGTESVIVTDESWKGH
                     IGGAIVMNDIYNGETYDATRRLTGWDLAGFDDGNWTTVRTGDYSLSNIIASEGAEIRK
                     IEELKPERIFRTPKGSLVADMGQNMVGWIRLKVKGPRGTTVILRHAEILDKYGEFYTD
                     NLRSARAQLTYTLSGDGEEVYEPRFTFMGFRYVEVTGFPGELAPENLTGVVIHSDMPM
                     TGSYESSNPLLNQLQHNIRWGQKGNFVDVPTDCPQRDERLGWTGDAQAFCRTAAFNYN
                     VAPFFTKWLKDVSADQRPGGEIPVVIPDVLDKQGSPTASASAGWGDVGVIAPWAMYLV
                     YGDRQLLGNQYPGMKAWVDYIAKKAGESMIWKGGSIYGDWLFYHPPVNNHPEPDGHTE
                     HDFIATAYFAYSSSLLSKAAGVLGKKEDEKYYSEIFEKIKKVFNHEYVTPAGRIGTNS
                     QTSYVLPLMFDLLPDDVRPNAARFLAEDIKSRGNHLSTGFLGTIYLCHVLTENGYNDL
                     AYDLLLQEEYPSWLYPVKMGATTIWERWDGQKPDSTFQDAGMNSFNHYAYGAIGDWMY
                     RVSAGLETLAPGYRHLLIQPHPTEKLDYSKASFVSMYGTIRSGWERMNGKIICRIEIP
                     VNSKATINLPAKSVEQIKESGRPVSVNKNLANVRLEGSTVKIEAGSGSYLFEISE"
     gene            17559..18587
                     /gene="rhaT"
                     /locus_tag="PK288_00090"
     CDS             17559..18587
                     /gene="rhaT"
                     /locus_tag="PK288_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HPM17346.1"
                     /translation="MNPVAGILLIALGSIGAASFYVPFKKVKEWAWESYWLAQGIAAW
                     LIAPWLFAIIFIPRGELIPILHEAPSSAKFMAVMFGILWGFGGLTFGLSIRYLGVALG
                     QSIALGLCAAFGTLIPAAVAGQNLFSSRAGILTLTGVALTVAGIAVIGYAGALKSREM
                     SEKDKRAAVREFALKKGILIAVFAGVMSACFNFGFEAGKPIEAAALAHGTNPLFQKNP
                     TLIFILLGGFITNLVYCLYLNIKNHTYRDYFRFPGNVILNNIMFTTLAGFLWFLQFHF
                     FGMGASKLPQGMAVFGWSILMALNIAISNIWGIILREWKGSGTKTIAILLIGIAVLIL
                     STFVVKLG"
     gene            18598..18912
                     /gene="rhaM"
                     /locus_tag="PK288_00095"
     CDS             18598..18912
                     /gene="rhaM"
                     /locus_tag="PK288_00095"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HPM17347.1"
                     /translation="MKRFGFKMKLYPGFKEEYRKRHDEIWPELAELLRKEGIGNYSIF
                     FDEETNTLFAYQEQKGGSSSQTLGNTEIVKKWWKYMSDIMETNPDYSPVTTPLEQVFF
                     ME"
     gene            19159..20424
                     /locus_tag="PK288_00100"
     CDS             19159..20424
                     /locus_tag="PK288_00100"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HPM17348.1"
                     /translation="MKKELIRKSYQLAKEQYAELGVDTEKALSEMDKLVISLHCWQTD
                     DVGGFEREGAELGGGGIQATGNFPGKARTIGQMRDDLDKVMSLLPGKQRLNLHAIYGE
                     FEGKKVDRDQIELKHFQGWIDWAKERSMGLDFNCTCFSHPLSDDGFTLSSKNPKIRKF
                     WIEHTKRCRAIAAEMGRQLGTPSVHNIWIPDGSKDTPVDRYTLRKNLKKSLDEIFEIR
                     YPVKYLKDAIESKLFGIGSESMVVGSHDFYLGYAIKNNKLITLDNGHFHPTEQVGDKI
                     SSILLFVDEILLHLTRGVRWDSDHVVTLNDDLMLIAHEIVRAKALGRINVGLDFFDAS
                     LNRVGAYVVGTRSVQLAFLSAFLEPTATLVKYEEDGKTFERLSLLELMKTMPLGAVWD
                     YYCMINDVPAGPDYIDEIRKYEKNVLLKR"
     gene            20474..21841
                     /locus_tag="PK288_00105"
     CDS             20474..21841
                     /locus_tag="PK288_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPM17349.1"
                     /translation="MKEKVIAVFDIGKSNKKLLLFDYNLRLVSENETSFPEQVDDDGF
                     ACDNIGAIEKWVRDSVSALSLSDKYDLTAMNFATYGATIAYLDKDGKRITPIYNYLKP
                     VDEKLPERLFRRYGGQDEFCRRTASPALGMLNSGIQIFWLKSEKPDIFSKVDQILHFP
                     QYISYLFTGKIYSEHTSVGCHTSMWDFDNMKYHPWLSDHGIKLPEPVSVMTCNEIESE
                     GKKIMTGIGIHDSSASLAPYFTGKRGNFLLMSTGTWCINMNPFNPEKLTTIQLDNDCL
                     CYMSVSMQPVKSSRLFLGNMHDNGVRILSDHFRTSGNYYKDLKYDNDLIQMLKGKYSG
                     DRKVFFSSESGSREFRDNIDLFEFRTFEEGYHQLMIELSELTIASVELILAENDATEN
                     IYITGGFSRNNIFLRLMAQAFPSKRVFTSEVKNGTALGAALVILQALGYPLPELNLGL
                     TEISS"
     gene            22106..23557
                     /locus_tag="PK288_00110"
     CDS             22106..23557
                     /locus_tag="PK288_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HPM17350.1"
                     /translation="MKDKHMFRINQPVPLALCASTFFLMSCTDSAEKRALPNIVFILA
                     DDMGYGDLSCYGQQKFSTPNIDRLASQGILFTQHYAGCTVSAPSRSSLMTGLHTGHTP
                     IRGNKEWDPEGQWPLPAASVTIAEMLKSCGYTTGAFGKWGLGFIGTEGDPNSQGFDEF
                     FGYNCQRLAHNYFPGHLWHNREKIILHDNDSGRTGSYGPDLIHRAALEFIKANREKPF
                     FLFYPTIIPHAELYAKDQYMAQFRGNFEPEKVFKGTDSGPGFRKGAYGSQPESHAAFA
                     GMIKQLDDYVGEIIASISELGLEKNTIVIFSSDNGPHLEGGADPDYFNSNGKFKGYKR
                     DLYEGGIRVPFIVRWPGKVKQGRISDHVSAFWDIMPTFAEAAGIDAPEETDGISFLPE
                     LLGRKQKDHEFLYWEFHEQGGRIAVRTGNWKAVKFNTELTDTGQIELYNLSVDPGETN
                     DVAGSNPEVVKEMLRIMKREHVPSDIFPMAAER"
     gene            23636..25165
                     /locus_tag="PK288_00115"
     CDS             23636..25165
                     /locus_tag="PK288_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HPM17351.1"
                     /translation="MRLCTEKKILLPVLMASVLPLSSSCRKAESPNIILIYADDLGYG
                     DVSCYGAKSILTPNIDRIARQGLQFSNAHCTSATSTPSRYSLLTGEYAWRRPGTGIAP
                     GDAPSVIKAGRFTIASLLKSAGYTTAVIGKWHLGLGPEGGPDWNGEIRPGPMDLGFDY
                     NFIMPATGDRVPCVFVENRNVLGLDPDDPIRVSFKEKIGSLPTGRENPELLKMHPSHG
                     HDMTIINGISRIGYMTGGTSALWTDEDIADVITEKAVRYIKDNKNRPFFLYFATNDIH
                     VPRVPHPRFAGKSGMGPRGDVILQFDWSVGEIIRTVEELDLSRKTLIIVTSDNGPVVD
                     DGYRDQAVELLGDHKPAGPLRGGKYSAFDGGTRIMFIASWKGKIKPGKSDALFSQIDL
                     MSSFASLTGQTIPAGEGPDSYNNLPVLLGKSRKDRSWLVEQSLNNRLSIIKGDWKYIE
                     PGPGIRIMPETGTETGNDPEPQLYNLRRDIGEKNNVARENQSIVNELTELLQEIRNSA
                     E"
     gene            25181..26683
                     /locus_tag="PK288_00120"
     CDS             25181..26683
                     /locus_tag="PK288_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPM17352.1"
                     /translation="MKINLKPALGIGVIASVPLVSCQNSGKQETSKPNILMILLDDYG
                     YSDVGCYGSKFYETPNIDRLASQGIMFTDAYASCPVSSPTRGSILTGKYPVNTGITEW
                     IPGRQATDYGAPYDKLLPLPFKLFLDPEEVTVAEMLKTKGYTTMISGKWHLGEDPAYW
                     PENQGFDVNKGGYKAGQPFVTATANGYFSPYGNPRLDNGPPGEYLTDRQTDEAIRFIE
                     NNREKPFFVLLSYYAVHTPLQAKEQHIKKFTEKADSMGLSSISPFTRDKDWIKPSMPD
                     DFRERIIQSNPVYAAMIWSVDENIGRVLARLEELGIDGNTVVIVTSDNGGLATSIGSP
                     TCNAPLRAGKGWLYEGGIRVPLIVRYPGLKSPAKVVDYPVSSVDFYPTFAEIAGYDCS
                     NEKIDGISFLSSLSGRNPEDRPLFWHYPHYNNRGVEPGSAIRLGKYKLIDNFERERQE
                     LYDLENDISETTDISASNPEKTRELFTLLNDWRALTGAKMMLPNPAWKKE"
     gene            26724..28202
                     /locus_tag="PK288_00125"
     CDS             26724..28202
                     /locus_tag="PK288_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPM17353.1"
                     /translation="MLKLNSVLTAGAVTLAGFQAPAQKPAALKAATPNIIIILMDDMG
                     YGDIGTTGANQYDTPNLDRLASQGMQFTWFYSPQAVSSASRAGLLTGCYPNRVGITGA
                     LMPWSTIGINSDETTIAEVLKTRGYHTAAFGKWHVGHQREFLPLQHGFDEYYGLPYSN
                     DMWPVDFDGVPIHLRDTTSRLMRYPVLPLIEGNEKIGEITDLSGQDKLTAEYTRRAVE
                     FIERHRKEPFFLYLPHSMVHVPLGVSEKFRGKSRQGLYGDVMMEVDWSVGEIVKAVDK
                     NGLAKKTLIIFTSDNGPWLNFGNHAGTTGGLREGKGTTWEGGQRVPCIMRWTGVIPAG
                     EICNRLASSIDFLPTVAAITGAKLPGKKIDGVNILPLMLGDKNTTPRHELYYYYKTNS
                     LEAVQRDYWKLVLPHRNNQSYRYVDPGKDGWPGPYGSETIYSPELYDLRRDPGEWYDV
                     AALYPEKVKELQDLAGEARRDLGDEITGDPGTGRRQPGTIKE"
     gene            28237..28788
                     /locus_tag="PK288_00130"
     CDS             28237..28788
                     /locus_tag="PK288_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPM17354.1"
                     /translation="MKFYLGYAALAGMGAVLGQDSAGPSPANERPNIIYIMSDDHAYQ
                     AISAYESILKDYAPTPNIDRIARDGMRFDRCLVTNSLSGPCRAVILTGKYSHVNGFIT
                     NDDQKPFDGSQQTFPKLLQKAGYNTAMIGKWHLESEPTGFDHWEILPGQGVYYNPDFI
                     TREGRHTEKGYVTDLIGKMSQLA"
     gene            28823..29800
                     /locus_tag="PK288_00135"
     CDS             28823..29800
                     /locus_tag="PK288_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HPM17355.1"
                     /translation="MMHHKAPHREWQPGPDELSLYKDVIFPEPETLFDDYAGRGTAEK
                     TQDMTISKTMRIEEDLKMYKDRSKMRRTGLDRMDSLQMAEWNKVYDPIISRFYESDLS
                     GNDLVRFKYQRYLQDYLACIAAVDKSVGEILNYLEKNGLDKNTVVIYASDQGFYLGEH
                     GWFDKRFMFEESYRTPLVIRWPGVIRPGSVNNDMVSNIDLPETFLEMAGLDVPSDMQG
                     RSMVPVLKGKTPSDWRKEHYYHYYELPGPHSVKRHYGISTDRYKLIHYYYDIDEWELY
                     DRKADPLEMKNVYNDPKYAKIRKDLHKRLTSLMKKFGDSDELARSFLPN"
     gene            29811..31280
                     /locus_tag="PK288_00140"
     CDS             29811..31280
                     /locus_tag="PK288_00140"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPM17356.1"
                     /translation="MKITFENILLIGSVLFLISIIAGKTSFRLGIPTLIFFLIIGMLA
                     GTDGIGKIEFNNPSIAQFIGIVALNFILFSGGFETDWKSVKPVFGMSFSLSILGVILT
                     AVFLGIFTWLVTDFTLFEGLLLGSIVSSTDAAAVFSILRSKNVALKGHIRPMLEFESG
                     SNDPMAYILTIMFLGMITGEVSSFSLGIKLFLMQLLIGGGCGFLFGYLSKQVINKIKL
                     DYDGLYPVLSITLMFLTYSLTNLAGGNGFLAVYLSALYLGNQNLLHKRTIMKMFDGLA
                     WLMQIVLFLALGMLVFPTHILPVVGVGLAISAFLMIVARPLACFICLAPFKIKTRVKW
                     FISFVGLRGAVPIVFATFPLMAGIDKAGMIFNIVFFISLSSVLIQGTSIPRVAKWLHV
                     DLPVNVKSVTPADVLLAESLNSQMVELTIASGFMAVGKKIVDLHFPPNVRISLIKKKG
                     IYVIPDGNTVIDSGDVLFIIGTNREALDSVTEMISERRA"
     gene            31355..32248
                     /locus_tag="PK288_00145"
     CDS             31355..32248
                     /locus_tag="PK288_00145"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPM17357.1"
                     /translation="MTYIFVEAQEFTIPLYNGPVPNSKNTGQAEIIEKNEIISIRNVQ
                     VPEIAVYLPARKFATGQAVIICPGGGYHILCYDYEGTDIARYFNSIGIAGIVLKYRLP
                     IYGNTLEPHKAPLMDAQRAIRLVRSNSSAWNIDPGKIGIMGFSAGGHLASCTGTHFDY
                     GRNDSDDPVERISCRPDFMILVYPVITFSDPVSHRGSREALLGKNADPELVKYYSSEL
                     QVQKDTPQAFLVHGDNDSVVPVSHSLLMYQALHNRKIPAELHIITEGEHGFGLAVDND
                     HPAAWTNNLEQWLKWLNRNRN"
     gene            complement(32283..33632)
                     /locus_tag="PK288_00150"
     CDS             complement(32283..33632)
                     /locus_tag="PK288_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase-maturation protein"
                     /protein_id="HPM17358.1"
                     /translation="MKSNLFFKDALKLRRPNAFHVMLKPAGPSCNLNCTYCYYLEKRK
                     LYRDTRNFHMSEELLENFTRQFIEAHDIPVVTFTWQGGEPTLMGLDFFRKAIELQEKY
                     RNGKKIENAFQTNGTRLDDDWCSFFHDNKILVGISIDGEEHNHDHYRKTFSGRPTFKR
                     VMKGIELLHRHKVEFNTLSVVNSYNVHFALETYRFLKRIGSGFIQFLPVAERSCDDIG
                     EDGLNLVSPGFAGVARVTEWSVGAGDFGDFLIAIFDEWVRNDVARYFVQIFDATLANY
                     VGENPGLCVFNDTCGDALVMEHNGDLFSCDHFVYPEYFLGNINDTGLIDLVRSQKQFD
                     FGIGKRNSLPEYCLRCEVRYACHGECPKHRFILTPDGKPGLNYLCRGYRRFFKHVEPY
                     MEFMAGELKGKRPPANVMQWIRNRESQVVRPELPGRNDPCPCGSGKKFKNCCLVKYF"
     gene            33759..35030
                     /locus_tag="PK288_00155"
     CDS             33759..35030
                     /locus_tag="PK288_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM17359.1"
                     /translation="MKRRRFLETASMGAAAAMSSIWLPPVTGESKLKIGLIGAGWYGM
                     VITKAALKTGETEVLGVCDVDSAHLEGSADEIEKIQGFRPRTFKYYEELLELRGLQAI
                     FIGTPPHWHALQFIAACEKGFDIYCEKPLAYDVQEGLAMVTAAKKAGNIVQIGFQRRQ
                     SEAFRKTKELIASGKIGKVYQINAQINYIPDPVDTTIQPPPPSLDWDAWCGPAPKLEY
                     RPSIGHMSWRLEKEYGNGHLVDWGIHHIDIIRYIMGEGMPAEFYSSGEINVLKGRITT
                     PDTLVARMTFSKAPVIWQHRLWGTGDVIREFNNGIMFYGDRGTLFAEDSKIILFDNNN
                     LREEIPAATPGMQEKHVANFIEAVRTRNMNLLTCTTEEAFMSTAAVQLAMISCYTDSL
                     VRWDVNSASITGNTEASRLLRREYRKGYTHP"
     gene            complement(35148..35852)
                     /locus_tag="PK288_00160"
     CDS             complement(35148..35852)
                     /locus_tag="PK288_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17360.1"
                     /translation="MNRIILVFFAIFLFAIQLSSQESDMIVVNAGTLIKSVFPPAIRY
                     RYPEFTEGLAVMKNGNVTKDRFNYNLLLGNMEFIKDRDTLSIVNKKDLKLITLGIDTF
                     YYFNGYLELIRSGTPRVYLKQLIALKEVRKTGAFGTTARSAAVDSHTYLATSGNITEL
                     RPEEDWVFQKTHEYYFSSGERPFVLFTRKNAIRSAPGHKQEIMKFIKDEKISFGSRDD
                     LLKLAEYLGSLPDVYP"
     gene            complement(35939..37045)
                     /locus_tag="PK288_00165"
     CDS             complement(35939..37045)
                     /locus_tag="PK288_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPM17361.1"
                     /translation="MKIKYIQFEILEDFMVKAMIRAGIPAADAAIVADVLIQADKLGF
                     DSHGVNRLKSVYLDRIRDGILNPVTKPEIIRETYTTAVIDGHNGMGHVIARMAMEMAI
                     KKAGNYGMGMVAVRNSSHYGFAGYYPLMAVRENMIGITGTNARPSVAPTFGVENMLGT
                     NPLTFGIPTDEPFPFLLDCATSITQRGKVELYAREGKKLRRGLVINDRGMTETDPEQI
                     LEELNDGTAALLPLGGIGEETGGYKGYGYATVVEILSSALQQGAFLKMISGISGSKKV
                     PYSLGHFFIALDVSAFTEPDDFRRTAGDILRELRNSRKMPGRSRIYTAGEKEYETWIS
                     RKDRGVPFSPELLNEFHGICSDYGLYEFADVFSI"
     gene            complement(37107..38510)
                     /gene="mnmE"
                     /locus_tag="PK288_00170"
     CDS             complement(37107..38510)
                     /gene="mnmE"
                     /locus_tag="PK288_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPM17362.1"
                     /translation="MPGREETICAPATAYGGAVSMIRISGRDALKIVDRVFYPADKNS
                     VTAGRKGYSIVYGEIRDGGEVIDEVLVSLFRAPHSYTGEDTAEISCHGSSYIISRILD
                     LLIRNGAASARPGEFTQRAFLNGKMDLSQAEAVADLVASSSKSAHRIAINQLRGGFSD
                     EISKLRAEFLQFASLIELELDFGEEDVEFADRNQLLALIGQTKALTDRLSASFRMGNA
                     IKNGIPVAIVGKPNSGKSTLLNFLLNEEKAIVTDIPGTTRDSIEDTVNINGLIYRFID
                     TAGLRDTSDVVEGMGIRKTLAIIARASVVMMVDESGERSSDINKRADEIRKMINDPEC
                     RFIILVNKADEASPSRINRLAREIRTEIDENLLFISAKYGTGIDKLKDYLSEITGANY
                     FSGEDVIISNIRHYEALQNISGCIGRIITGLESKLPEDLIAIDIRHAIHYLGEITGEI
                     TTDEILGNIFKNFCIGK"
     gene            38724..39626
                     /locus_tag="PK288_00175"
     CDS             38724..39626
                     /locus_tag="PK288_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17363.1"
                     /translation="MKNDRSEIKSDYISATFLLPDAENGYYRGTRFDWSGVISDLSCC
                     GHTYFGKWFDNYSPTLHDAIMGPVEEFAPLGYDSADPGGNFVMIGIGLLKKSDDQAHN
                     RFGYYQITDPGKWKIKTRGDEISFNHVLKSEVNSYDYTKTVRLAGDKPIMEIDHKLRN
                     TGKTRIVTNVYNHHFFVIDNQPVGPDFTINFPFKLSGEGQGFGSIAELNDNSIIFNRV
                     LEKDETVFCGSLTGFSSNPEDLDVRIENKSAGAGVRITGNRPLSKLIFWACRTTPCPE
                     TYVDINIEPGKEFTWKFIYEFYSF"
     gene            complement(39761..40687)
                     /locus_tag="PK288_00180"
     CDS             complement(39761..40687)
                     /locus_tag="PK288_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005850760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPM17364.1"
                     /translation="MKYSIIKQCIALSLAVLFTFCAGPGSRKSRTGWKAGHVVLIGLD
                     GWGAYSVEKADMPNVKQLMQNGSFTLSKRSVLPSSSAVNWASMFMGACPELHGYTEWG
                     SRVPELPSRVLNRNGIFPNIFQLLRDAAPDAEIGMLYDWDGIKYLSDTLTLNYYAQAP
                     DYSKNPSGLCDMAVEYIRDKKPVLAGIIFDNPDHVGHVDGHDTPAYYENLKELDGYIG
                     RIIAAMKEAGIYENSIIIITSDHGGVGKGHGGKTMLEMETPFIISGKNIKKGGQFDDS
                     MMQFDCASTIACIFNLEQPQVWIGRPMLQVFK"
     gene            complement(40925..41704)
                     /locus_tag="PK288_00185"
     CDS             complement(40925..41704)
                     /locus_tag="PK288_00185"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPM17365.1"
                     /translation="MTRTFFLMAAMLTAFLLPLKAQQSDPVQLMDKSRDLTMSGNLQS
                     TVILTITEKNGAVRTRKVNMVSKTYGETEKRLMKFLEPADVRGTAMLIIDNKNTQDDM
                     WIYLPALKKTRRIVSNEKGKSFMSSEFSNADMTSGSNTDFNIRHMPESGSNGLWVIES
                     KPVNDKVADEYGYSRKVTTLDKNDLKIKKIDFYNFDGTLFKTIEINATVPRPGSREAY
                     IMTDMFARNHINGRTSRVQFEQINTSASIPDNTFMSENLSR"
     gene            complement(41631..44021)
                     /locus_tag="PK288_00190"
     CDS             complement(41631..44021)
                     /locus_tag="PK288_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPM17366.1"
                     /translation="MADFIIKYRWLIISVSLAVALSLGLLIPTVETDPEIRNFVPHSM
                     PSRINTDSIEKDFGVQDMIIVILEDSSILTQGNLERLKEIDRGFSRLEGIGTVNSLFT
                     ARKITGEDGMMVVDPLVRQIPDTPDKMEALRRDILENRFALGIVASQDMTAAAVTGTV
                     NPAIPEHLTLDRVDSIAKAQENGAEVLTGGLPYIRRHIVSDVNHDALILIPIALIIML
                     SVLKLSLGKWKSVLLPFSVVVLTVTATMGLIPLLGWKFSILSLLVPIILISVANNYGI
                     YFVSKHQELKSLETPPGRRDLIRKITGSLNMPILFSGLTTIAGLLGLLAHSIIPARQV
                     GVLAATGVSLALLMSLLLIPSVIWLRERPSKGKDPVRSLIPLKRKKERDLSKDLLSRG
                     LAQLSDLVVGKPGRVLLVSAVITVVISTGIAFLRIDTNQENYFPQNHPVRKASRVINS
                     KFGGSQTISVMVSGDIKDPVVMKKIDELTSHLKSQDGVGNVFSISDVVREMSKALYDP
                     GENGYDKIPDSYEAIAQMFELYNMSGNPDDWKQLMNFENTRAHLLVKLSKPDNHVISA
                     LRNDIRSFTAGFPAEVTVGGYAIIMADFANKIIRGQISSLIFALVTVFVLLSVIFRSV
                     RGGLIGSIPLGVSIIIQFGIMGYANIPIDAATALLSSIMIGVGVDFTIQYLWRYNTEF
                     RRSLSHAKAISVSLTTTGRSIVINALSVMAGFSATFISGFLSIRYFGYLVLISIGSCL
                     LCAIIVIPAFMLWFRPAFIEADLNKTSKNNDKYDKNIFSHGSNADSIPAALESTAV"
     gene            complement(44026..45372)
                     /locus_tag="PK288_00195"
     CDS             complement(44026..45372)
                     /locus_tag="PK288_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17367.1"
                     /translation="MTKRFLFTLIFTALIFNLSAQEKVFTPGGFLRGGMFYSTGDYPN
                     DVNAAFADAAFTLTATDNMSFKGFGDIRLRMGQQFGDNVNSLVVREAWGMYYNRFISI
                     SLGKRIIKWGKTDIFSPLSRFSPLDYTYRSPDLEDKDMGNLAGELTITPASFLKLTAV
                     ATPLWHPSVLMTAPLELPPNINLSMPEGLYRGNGYYSLGFRGDLLLSGFDAGIQWYHG
                     PDLMPGLSLVSADFSNLLNPQIAIAGVPYIINHTGLDFEVPVSPLVLRGALAYSKPVR
                     KKEGNEEIPFPQIDWVAGADWTPGNFRMTFEYSGRKVLDYYPAPYDPVIGTEPDLVKL
                     SQLFATPGFDPVEFTRLQTEAFGRLYNNQVYEYYHSAGLRFEAELFYGRLLPSVMTIY
                     NFTSKDLLLIPMIRFKPADGLTIGAGMEHYSGKKGGLYDIVDNFMKAAFVSIKIDF"
     gene            complement(45482..46612)
                     /locus_tag="PK288_00200"
     CDS             complement(45482..46612)
                     /locus_tag="PK288_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM17368.1"
                     /translation="MVNIEPVLYIGISQSFFAGLLISTKKPVTVANRLMAAWLFMICI
                     EMIFALVNSRVIEMYSFPFITFTYGPLLYLYIRFMTVPERKFLWTGLLHFIPFLVFFT
                     ISVVFRSEPLVRDLRGFFKPDKLMPLRIVYSVVFFLSITVYSILAFVEIRKHQSNLRN
                     LISYTSQKMTLNWLKILTVSFYVAYFVLFILGGLNIIGNYIPFDPYFVIFGFIAIFSF
                     VYSFYGIKQPSIFGEVVHQNSRDEVSERADPEKYSRSGLRDEQADEYLKILLGYMETE
                     KPFLNGDLTIHDLSEATGISRHHITQVLNEKYKKNFFLFINEYRVREVIKRISDPRYQ
                     HFTILAIAFDSGFNSKSTFNNFFKTYTGKTPSAYRELIHKSE"
     gene            46983..47858
                     /gene="sucD"
                     /locus_tag="PK288_00205"
     CDS             46983..47858
                     /gene="sucD"
                     /locus_tag="PK288_00205"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPM17369.1"
                     /translation="MGILVSRKSRVIIQGFTGSEGTFHAQQMIDYGTNIVGGITPGKG
                     GRLHLGLPVFNTVREAVRSTGADVSVVFVPPAAGADAIMEAAEGGIRLVVAITEGIPV
                     SDMVMVKEYLKSLNCRLIGPNCPGIITPGEAKAGIMPGFIHKKGHVGIISRSGTLTYE
                     AVDQVTRLGLGQSTCIGIGGDPVVGTTTLEAVKLMMNDDDTHAIIIIGEIGGSMESEA
                     AEWISANGTKPVVGFIAGQTAPKGRRMGHAGAIIGGRDDTAAAKIEIMKKCGITIVES
                     PADIGSSVLNVLEKR"
     gene            47998..48744
                     /gene="fabG"
                     /locus_tag="PK288_00210"
     CDS             47998..48744
                     /gene="fabG"
                     /locus_tag="PK288_00210"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPM17370.1"
                     /translation="MKLLEGRTALITGASRGIGKAIAHKFAREGANIALTNIYEDEEF
                     LSTINEIKLYNVMVKGYVSNASNFADSQKVVDEIVKDFGQIDILVNNAGITKDTLLMR
                     MTEEQWDAVIAVNLKSVFNFTRAVIQPMLKQRNGSIINMSSVVGVSGNAGQSNYSASK
                     AGILGFTKSVAKELGSRNIRCNAIAPGFILTEMTDNLPPDVKAEWTNRIPLKRGGTPD
                     DVANTALFLASELSSYITGQTIHVCGGMLT"
     gene            48871..50097
                     /locus_tag="PK288_00215"
     CDS             48871..50097
                     /locus_tag="PK288_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPM17371.1"
                     /translation="MNNPLFLLIVLIPVSGFLLERYLVFLNSKMWSDKLPEELTGICD
                     EAEYRKTLHYEKDNRKLSFWSSSFNLAIILAMIIMGGFAFFDKLAGSISTNMIVISLI
                     FFAFIGIGSEIINIPFKWYDTFVIEKKYGFNTMTVRIFITDHLKSWFITMIIGIPVLA
                     LITWIYYRLGKNFWLWAWGLVTVLSVFINFFYSELIVPLFNRQTPLPESSLRTRIETF
                     ARETGFRLRDIYLIDGSKRSTKANAYFSGFGSKKRIVLYDTLLKEMSEEEIVAVLAHE
                     IGHYKKKHVLRRLLSSIFITGFMLFLFSLVVDNPALSEALGAGRPSFHIGLVAFGILY
                     SPLSLVIGLAGNFLSRRNEFEADLYVRQNYNPEFLASALKKLSVKNLSNMIPHPAYVF
                     FHYSHPPLLARLERLK"
     gene            50122..50194
                     /locus_tag="PK288_00220"
     tRNA            50122..50194
                     /locus_tag="PK288_00220"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50155..50157,aa:Thr,seq:cgt)
     gene            50380..50452
                     /locus_tag="PK288_00225"
     tRNA            50380..50452
                     /locus_tag="PK288_00225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50413..50415,aa:Gly,seq:ccc)
     gene            50778..51359
                     /locus_tag="PK288_00230"
     CDS             50778..51359
                     /locus_tag="PK288_00230"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPM17372.1"
                     /translation="MSYTLKPGQKAIDFRLPATDGREYSLSDFDKYPYLVLFFTCNHC
                     PYVTGSDEVTRATAEKFRDRGVVFAGINSNSENTYEEDSFPNMVRRMKQHNFPWKYLH
                     DREQTVVKAYGGLRTPHFFVFNAERILVYTGRGVDSPRDISRMTVNDLDRALEELTSG
                     REISVPVTNPIGCNIKWEGMNAKWMPPEACDLV"
     gene            51463..51999
                     /locus_tag="PK288_00235"
     CDS             51463..51999
                     /locus_tag="PK288_00235"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPM17373.1"
                     /translation="MNLINIQYCKTRIGELILGSYAGRLCLMDFRYRKMRPAVDNRIK
                     KGLAAEFREQNDEIIAEAIRQLEEYLAGHRTAFDIPVLMIGTDFQKDVWRALTEIPYG
                     KTVTYTELAKAIKQENAVRAVAGANGANAIAIIIPCHRVIGSSGELTGYGGGLSVKKR
                     LLKLERNNSVGQLPKSIF"
     gene            complement(51983..52387)
                     /locus_tag="PK288_00240"
     CDS             complement(51983..52387)
                     /locus_tag="PK288_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17374.1"
                     /translation="MNDFMCPKCKGHLRVGDHIIFKIKNDRKETGLILLSPQIGNYSS
                     IKHPSFEFGQGELVDFYCPLCSASLKSDIHKNLALVLMKDQEGKGFAVYFSQVAGEHS
                     TYEVDGDSVRAAGEDAGLYTYFKIGEKFKKYF"
     gene            complement(52669..53061)
                     /locus_tag="PK288_00245"
     CDS             complement(52669..53061)
                     /locus_tag="PK288_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17375.1"
                     /translation="MKFRIFFSGVFLIFLFSGGCEKTESGRSVDSIVNHRFGVDANLA
                     FAIDSIRDYRCPSDVVCVWAGDVDVFMKFYLASASIDTMMNLNNAARNPVSIGGYSFR
                     INEVLPYPVSNRTTDQKDFKISMTITRE"
     gene            53273..54517
                     /locus_tag="PK288_00250"
     CDS             53273..54517
                     /locus_tag="PK288_00250"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPM17376.1"
                     /translation="MKAEIITIGDELLIGQTIDTNSAWIGWELSSAGFDVRRRTSIHD
                     NRDEILNALSEAAERKPELVIITGGLGPTSDDITKQTLCEFFNTKLVPDREVLEMIEK
                     MLRRRNFPMNENNRRQAEVPESCRVLPNAAGTAPGMWFEKDGTIFISMPGVPGEMKYI
                     MQEHVLPDIKKRFRSQVIIHKNIMTYGMPEARLAEILEQFEAELPENIKLAYLPSFGV
                     IKLRLTGTAGTLNEIEPLIGAQTSKLYNIIPDLIFGEDEQTPEMAVGQLLKIKKLTLS
                     LAESCTGGFLAHRITSVAGSSEYFAGSLVAYSNNVKTGILGIDPAILASDGAVSSRVA
                     AGMAEGARKLFKTDLGLATTGIAGPSGGSADKPVGMVWIAVSSDSGTITEKHIFSTDR
                     IANITRFSLAAMNLLRLQIINR"
     gene            54633..54875
                     /gene="rpmB"
                     /locus_tag="PK288_00255"
     CDS             54633..54875
                     /gene="rpmB"
                     /locus_tag="PK288_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPM17377.1"
                     /translation="MSKICQVTGKKAMVGNHVSHSKRRTKRKFRPNLSVKKFYLPEED
                     RWISLKVSAAGIRLINKKGLNAVLKEAREKGYILNH"
     gene            54887..55075
                     /gene="rpmG"
                     /locus_tag="PK288_00260"
     CDS             54887..55075
                     /gene="rpmG"
                     /locus_tag="PK288_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPM17378.1"
                     /translation="MAKKAKGNRLQVILECTEHKESGMPGTSRYITTKNRKNTPDRLE
                     RKKYNPILKKYTIHREIK"
     gene            55088..55243
                     /locus_tag="PK288_00265"
     CDS             55088..55243
                     /locus_tag="PK288_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HPM17379.1"
                     /translation="MAKKVVATLKIGSGKLFTKCIRMSRSDKTGAYQFTEEIVHNDHV
                     KEFFNKK"
     gene            55330..56289
                     /gene="ftsY"
                     /locus_tag="PK288_00270"
     CDS             55330..56289
                     /gene="ftsY"
                     /locus_tag="PK288_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPM17380.1"
                     /translation="MGWFGFVSKDKKELLDKGLEKTKESVFTKLSRAVMGKSRIDDEV
                     LDNLEEVLVSSDVGVDTTLRIIERLENRVSRDKYLNTDELNHILKEEIVSLLEANISG
                     TEYDFASELPFKPFVILVVGVNGSGKTTTIAKLAYQFRNAGKKVLLGAADTFRAAAID
                     QLQIWADRAGVQVVKQQMGSDPASVAYDSVRSAVAGKYDVVIIDTAGRLHNKVNLMAE
                     LSKIKRVTEKILPESPHEVLLVLDGSTGQNAFVQAREFTAATDVTAMAITKLDGTAKG
                     GVVIGISDQFRIPVKYIGLGEKLDDLQIFNPYEFVDSLFSAAR"
     gene            56328..57623
                     /gene="rimO"
                     /locus_tag="PK288_00275"
     CDS             56328..57623
                     /gene="rimO"
                     /locus_tag="PK288_00275"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPM17381.1"
                     /translation="MINRTINIVTLGCSKNLVDSEKLTRQIEAAGYRVIYDGAELSSN
                     IVIVNTCGFINDAKEESIDTILRFVEALKKGDIEKLYVTGCLSERYAAELRSEIPEVS
                     RYFGVNSMDQILRELGGHTDKRLLLERRISDPGHYAYLKVSEGCSRTCAFCAIPSIRG
                     KYLSRPVEEVVEEARILARNEVKELILIAQDLSYYGLDLYRTQMLPVLLRELLGIESF
                     RWIRLHYLYPSNFPTGIIPMMQENRRICQYIDIPVQHISDRILKMMKRSHNGSETREL
                     LATLRKELPGAAIRTTLIAGHPGETQKEFSLLKDFVGEFRFDRLGVFPYSHEEGTYSY
                     SNYRDSIPEKIKKERVSELMELQQSISSDLNTLKINKILKVIIDRREGDMYIGRTEHD
                     SPDVDQEVLINAVHDLKPGNFYNILITRSTEFDLFGTPV"
     gene            complement(57649..>59461)
                     /locus_tag="PK288_00280"
     CDS             complement(57649..>59461)
                     /locus_tag="PK288_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPM17382.1"
                     /translation="RITKKNVSRVLFNKRVVALDLASIVAGTKYRGQFEERMKAILNE
                     LSKNDNIILFIDEIHTIVGAGGATGSLDAANMLKPALARGEIQCIGATTLDEYRQHIE
                     KDGALERRFQKIIVEPTSIEETINILHNIKERYEEHHNVIYTDDAIDACVKLTNRYIS
                     DRHLPDKAIDALDESGSRVHISNIVVPERILNLEKQLDDTKKEKMMAVKNQNFEKAAS
                     FRDKEKDLIDMIEHEKKKWEKELAVNRESVDAEKVAEVVAMMTGVPVQRIAQTEGTRL
                     LKMAEELKGSVIGQDEAIQKIVKSIQRNRAGLKDPNKPIGTFIFLGPTGVGKTQLAKV
                     LAKFLFDTTDNLIRIDMSEYMEKFSVSRLVGAPPGYVGYEEGGQLTEKVRRKPYSVVL
                     LDEIEKAHPDVFHIMLQVLDEGQLTDSLGRRVDFRNTIVILTSNIGTRQLTEFGRGIG
                     FDTSARKASRDEQAKSILQKALQKTFAPEFLNRIDDVIMFNSLGKKEIDKIIDLELKG
                     LFDRVNALGYKMKIVPAAKDFIAEKGFDANYGARPLKRAIQKYLEDPMAEVLIKADLK
                     PGDTIHIGFNSQKSEIRIKIHKNNQESPEGIPDPPAE"
BASE COUNT        16917 a        13520 c        13407 g        15617 t
ORIGIN      
        1 tgaagccatg caggaacgac agattaccat cggagaatca acctatcatc tggaagagcc
       61 atttcttgtt cttgcaactc agaacccgat agaacaggag ggtacatatc ctcttccgga
      121 agcacaggtc gacaggttta tgatgaaagt actcattgac tacccttcca ttgaggagga
      181 aaaactgata atccgtgaga acctgggaaa agagtttccc aggcccgata cagtactgaa
      241 gccggaggac ataatcaggg cacgtgatat agtgaaggag gtctatatgg atgagaagat
      301 tgaaaactat atactgaaca ttgtttttgc aagcaggaat cccggaaagt atggtttgcc
      361 atcatataac agcatgattg cttacggcgc atccccgcgc gcgtccatca accttgcact
      421 tgcatccaag gcatatgcgt ttatcaggag aaggggttat gtaatccctg aagatgtcag
      481 aacaatatgc gccgatgtta tgcgccacag gatcggactt acctatgaag ctgaagctga
      541 aaacattaat caggataaga ttatttctga gatacttaat gttgttgagg ttccgtgaca
      601 cacaaaagct tcgggatatt aacggcatga gagacggttt tttgcggtaa tcaaacctga
      661 aggtcttgca aggtggaagc aattgaatta ttaaagaaag taagacgaat agagatcaaa
      721 acccgggggc tgaccaggca gatctttgcc ggagaatacc atagtgcatt caagggccgg
      781 ggcattgcct tcaatgaggt gagagaatat cagtatggcg acgatatcag gaatatcgac
      841 tggaatgtta cggcaaggtt taatcatccc tacgtgaagg tttttgaaga agaaagagaa
      901 ctcaccgtga tgctgctcat cgacctgagc ggatcgggaa atttcggaac aaaagtcagc
      961 ttcaaaaggg atctcatgac ggaagttgca gcagttcttt cattttctgc catctataac
     1021 aacgacaaga tcggtgtgat ctttttctcg gaaacagtgg aaaagttcat cccgccccag
     1081 aaaggcagaa agcatattct ccgtattata cgggaactgc ttgatttcaa gccacgcagc
     1141 aataatacca atctgggcga acctctcagg ttccttacca atgcaattaa gaagagatgc
     1201 acggctttca ttatatccga tttcattgca ccagattttg atgaagccct gaaaatcgct
     1261 gcaaataagc atgatgttgt agcgctaagg gtatatgatc ctttcgaaag ggaactgccc
     1321 gatatcggat tcgtaaaggt aagggattct gaaacgggta ctgagaaatg gattgataca
     1381 tcctcgtctc tgacaaggaa ggcatactcc aggtggtggt ccgaccacac cggaaatgtt
     1441 gaaggcacat tcagaaagta tggcgttgac tccgtgagcc tgtcaacagg agaagattat
     1501 gtaaaaccat tgataaagct ttttgaaaac agatgattac acaatctttc agatcatatc
     1561 tgttgggttt gttgtttctg atataccggt tggcaaacct gaactgccag gatataactg
     1621 ttatcacaaa atttgataca tcaaggattt acattggcga ccagatcaat ttctccgtaa
     1681 ctgttgaaca gccggcccgt ttgcagatac cctttcctgt tttcagggat accctggtaa
     1741 aaaacattga aatacttgca gggcctgttg tagatacaac aatcgttgcc gacagtctga
     1801 tcagggtaac gggccaatac cttgtcacct catttgattc cggctattac agggttaacc
     1861 ctgtctatat tgaactcagg gatgccgggg gtgtaaaaag gtattattcc gattattcag
     1921 ttctggaagt tgccagggta aaagccactc ctcctgatac agtgaaagga atattcgata
     1981 ttgtaaagcc ttacagggct cccgttaccc tcggtgagat tatgccatgg atcctcctcg
     2041 gaatagtggc tgtggcggct atatggttca taataagatt cggcaggaaa cttattaagc
     2101 caaaggcaag tcttcctgag gtcattcctg tcgaagctgc ccatgttatt gcactccggg
     2161 aactggagcg gcttaaggat gaaaaattgt ggcagtcggg tgaaactaag aaatattata
     2221 cacggctcac cgaaatactc cgtaaatatc ttgaaaacag gttcggtgta ttttcgcttg
     2281 agatgacaac atctgaaacg cttgatgcac ttatcaggac cggattcaaa aaggacgaca
     2341 attacaacct gttgaaatcg gttcttaacg gtgccgatct ggttaaattt gccagatata
     2401 aaccggaggc atccgaaaac gaaatccatt atgaaaatgc atggaatttc gttttggcca
     2461 caagaaaaga agaagtatcc gaatcaccgg cttcaggtaa taacttgaat aaagatacca
     2521 gcacatgaac gggataatat ttgcggatcc gaatttcctg tacctgctgc ttctggtgcc
     2581 ggtgattatc gctttttatg ttctgaagca acataatgct tctgcatcat taagtatgcc
     2641 tgagctgcaa cctttcaacc aggccggcaa gactttccgt aattacctga gacatgcctt
     2701 gtttgcattc agggtactgg cttttgtttt cctgatattg gtcctggcaa ggccgcaggc
     2761 aaccaatacc tttcaggaca tgtcaacgga aggaatagat atcatgctga caatggatat
     2821 ctcggggagc atgcttgcaa gggattttaa gcccgacagg cttgaagcat ccaagaatgt
     2881 ggccactgaa tttatttcag ggaggcccta tgaccgaatc ggactggttg tattcagcgg
     2941 tgaaagtttc actcagtgtc cccttacaac agatcatgcc gtcctgataa atctgatgcg
     3001 ggagatcaaa agcggcatga ttgaagatgg gactgctatc ggtatgggac ttgcaaatgc
     3061 aataaacaga ataaaggatt ctgaagccaa aagcaaagtg attatccttt taaccgatgg
     3121 tgtcaacaac aggggtgaaa tagctccggc cactgctgcc gagattgcaa aaacatacgg
     3181 aataagggtt tacacaatag gtgtcggaac ccagggcatg gctccctatc ctgtccagac
     3241 accattcggt acccgctacc agaatatgcc cgtagagatt gatgtggcaa tactgaccca
     3301 gatagcacgg gaaacaggag gcaggtattt cagggccaca gataacaata aactggtgga
     3361 agtttacaat gaaatcgaca agcttgaaaa atcaatcatc gatgtcagac aattcagcaa
     3421 aaaggaagaa aaattccttt ggcctgcatt cgccgctttc tgcctgatgg cactggaatt
     3481 gatactgcat tatacgttat tgagaaacct aacttaaaac cagacaatca tgcagttgtt
     3541 cagatttgcc aatcctcagt acctgtatct tttacttatt ctgccggtaa tgcttgtcat
     3601 tttcctcctg aataacctgc gcaggaaaag attgatcaga aaaatcggca cccccgctct
     3661 tgttagtaaa ctgattccgg aaatgtcggg aaacagaccc ctgattaagt tcctgattat
     3721 gatgcttgcc gtgtcttctg cagtgataat gcttgcccgg ccgcaatacg ggtccaaatt
     3781 agaagaagtt aaaaagcagg gagtggaggt cataatagca cttgatgtca gtaattccat
     3841 gctggccgag gatattcagc ccaacaggct ggtaagggcc aaacaggcaa tctctaggct
     3901 ggttgacaac ctttccaacg acaggatcgg attgatagtc tttgcaggtg atgcctatac
     3961 ccaggtgcct gtaaccaccg attatgtgtc ggcaaaaatg ttcctttcca caataacacc
     4021 agatatggtt gcaaaacagg gaacggctat cggggcagca atcgaactgg gaataagatc
     4081 attcagcccg ggtgaaggta aaagcagagc catgataatt attactgacg gcgaaaacca
     4141 tgaaaaagat ccgttatcat ctgcagagga ggctgcaaag gcaggaataa taatccatac
     4201 gataggaatt ggctcaccag agggagttcc gatacccatc agcagcggag gaaggaaaga
     4261 ttatctgaag gatactgaag gcaatacggt aatcaccaaa ctcgatgaag aaacgcttaa
     4321 aaatattgca ctcagaacag gaggcagcta tgtccgggcc agcaattcaa gtctgggcct
     4381 ggatgaaatt ttcagcaata tcagaaaaat gaaaaaggaa gaactggaag gtacgattta
     4441 taccgaatat aatgaccagt tccggatttt cgcaggaatt tccgctatat tccttatcct
     4501 tgaattcttg atcatgaaca gaaaaaaccg caggctggca aacgtcagat tatttaaatt
     4561 taagatctaa ttttgtaacc atgcatatcc tgagacacaa atacttgatt tctgatcatg
     4621 gtattgtact gacattatgt atattatatc tatttcccgc aaccctgacc ggacaagctg
     4681 aaaaaaaatt catcaggcag ggaaaccggg aatatgaaaa ggaaaaattc gccgaatctg
     4741 aaatttcata ccggagagcg cttgataaaa acaaagcatt tcccgatgcc gtattcaata
     4801 ttggcgatgc tttgtacaaa caggaaaaat atgatgatgc agtgcggcag tttgaaggca
     4861 gctcggcaat gacggataat cccgataaaa aatctgaggc attttataac cagggcaatt
     4921 cgcttctgat gggcgggaag ctgcaggaaa gcattgaagc atacaaaaat tccctgagat
     4981 taaaacccgg taacctggaa gcaaaatata atctcgcttt tgcacaggat ttgctaagga
     5041 aacaagagga acaaaaaaat caggacaaga ataaacagga taacagggaa caaaaccagg
     5101 aacggcaaaa taatcaggac aaggaaaaca acgataagga tcaggagaaa cagcaggaac
     5161 agaataacca gcagcaacaa caacagcagc agcagcaaca acaacaacaa caacaacaac
     5221 agcagcagca ggagcaacag gggatttcaa gggaggatgc tgaacgtctg ctgaattcaa
     5281 ttgcaaatga cgagaagaat gttcaggaga aagttaaacg ggcaaaagtg gctgacacaa
     5341 gggttagaac cctgaaaaac tggtgaccgg aaatgaaatt acacaggttg atattatctt
     5401 tattactgat tgttctgcgt cttaactcgc agggtcagga tatcactgtc gcagccgatt
     5461 atcccggcgt tgttcgtaca ggggaacaat ttacgataac ctggacgatt aattccggag
     5521 gaggtgaatt tgttccaccg tccttcgatg gattctataa gctgatgggg ccacagacct
     5581 catacagttc aagcactcaa atcattaatg gcaagatcac cagggaaacc acatattcct
     5641 actcatatta tctgcaggcc gtcaactccg gcaacttcgt ccttgccccg gcaactttta
     5701 tttacaagaa ccggacctac agttcggatt ccatccggat agaagtcaca ggaaccggaa
     5761 gcaaccagca gggaagaaca atgccggggg cttcaggaaa tgaaatcact gagcccgtca
     5821 gggaaacaaa agatgatatt tttctgaatc tgtctgtaaa taaaaaagat gtctatatcg
     5881 gtgaacatat agtggccacg gtgaagatct atacaaaagt tgatatttcg ggaataagcg
     5941 aaataaaata tcccggattt gaaggatttc tgagaactga tctgaacaca cctccactta
     6001 cttctttgca aagagagaat gttaacggta caatatacgg aacagggatt attcagcagt
     6061 ttctgatata tccccaggta tcgggagaga taacaatcga tcccgttcag ataaaagtcc
     6121 tgattcaaca gaaatcaggc aggtcagatc cgttcttcgg tgatttcttc gccacctata
     6181 caaccgtccc aagagttgtt gtaagccctg ctgtaaaaat aaagtcgagg cccttgccgg
     6241 aaacaaagcc cgatgacttt tcagggatcg taggcaaaat atctctggca gcatctcttg
     6301 ataaagactc tgtaaaagtc aatgacgcag tgaatctgaa aataatcctg tcgggaaccg
     6361 gaaacctgag actggcaaat gctcctgatc tgaaattacc ccctgatatt gaagtatacg
     6421 accccaaaat atccgatgac ctcaaaaaca cgaccgaagg gacctcagga agaaagatat
     6481 tcgaatatct gctgattcca cgtcatcatg gagagtattc aattccgccg gtgtcatttt
     6541 catattttga tgtttcaact aagaaatacg aaaagcttac agtccccgga ctgcactttt
     6601 atgcaagccg gagtgatcag ccaggaaccg ggataacagt atttggagga gtttcaaaag
     6661 aggatgttaa atatctgggc agggatatcc ggttcataga ccagaatccc ggatcactca
     6721 ataagccgat gaatatcctg acatcgaaac gatcgtttta cacagcatat gccggagctt
     6781 ttattttgtt cctcgcaatt ctttttgtca ggagggagca tgtaagaagg aattcggaca
     6841 ttgctatggt ccgcaacagg aaagctgcga aagttgcggg aaagcgactg aaaacagcaa
     6901 aagcctgcct gaaggagggg ccttccgaca cattttatga tgagatactt aaagcaattt
     6961 ggggttatct gagtgataag ctgaacatac ccgtttctga tctgaccagg aaccgggccg
     7021 ttgaatcact ccgcgaaaga ggcatcgcag aagaacagat cgacaaactg gtctcggtac
     7081 ttgatgcatg tgaatatgcc agatatgctc catcttcatc aggaactgag gcagccaggc
     7141 tgtatgaaga tgcatcagat tttataagtt atgttgaaaa tccggtacgt tgaaatgaag
     7201 catattaaaa ttatcctgtt tcttttgatt tttttccttc cgacagcgag acctgctttt
     7261 gcacaggagt tgcccgcaga aaaattcagc aggggagtgg aatactacaa atccgctaat
     7321 ttcaaggaag ctctggatgt atggatggat atctacaata caggttacag gtctgcagct
     7381 ttgtactata atatcggcaa tgcctgcttc aaactgaata atgtccccgg cgccatcctt
     7441 ttttatgaga gagcaaagct ccttaaaccg ggagatgaca atatagaata taatcttcag
     7501 attgccagaa cgatggttgt tgataagacc gaagagatcc cgggattgtt ttttgtgaag
     7561 tggtttgatt ttatctcatt gctggtgaca accaataatt gggccatgat aagcctcgct
     7621 gctttcattt tcgccctgat atcatcttca atataccttt attcctcctc atacagactg
     7681 aaaataatca gtttctggct tgccctggct ttatttgtac tttcagcctg cacattgtca
     7741 ttttcattca ggaacaggtc cctggtccat aacagccggt ccgcaattat cttttccccg
     7801 tcggtaaatg gaaaaagctc tcctgatgca agcggaacgg atctttttat ccttcacgag
     7861 ggatcgaagg ttacaatcgt ggatgaggtc ggtcagtggt atgagatcag attgtccgac
     7921 ggcaacaagg gatgggttcc atccgactgt ctaagcaaaa tctaggttta taaacggctg
     7981 cccggaattc ctctttcagg gaattcactt taggggtttc agccttacgc atatatggaa
     8041 catatgcaag tcagatccgt cagatgtctg aattactgcg gaaatactcc ccatcatgcc
     8101 cagttaatgg gagtaatccc attcatcttc aggtattcat tgcacttgct gaaatgcctg
     8161 tttccgaaaa atcccctttg tgcagacaga ggtgagggat gaacggattt aagaaccagg
     8221 tgcttctgat agtcaatgga ttctcccttt ttctgtgcat aagcccccca aagaaaaaaa
     8281 accaggttct tcctttcatc attaagcacc tttattacgg aatcggtgaa ctcctcccat
     8341 cctttcttct gatgagagcc agcagcatgc gcccttacgg tcagggtggc attaagcaac
     8401 agtactccct gcgaggccca tctttcgaga tttccggatg gcagcgggtc gatgcccaga
     8461 tcagagccga tctctttaaa aatattgacc agactgggag ggaactcaat cccgtcacgc
     8521 acggagaaac ataatccatg agcctgccct ggtccgtgat aaggatcctg tcccagaata
     8581 acaactttta ccctgtcgaa cgggcaaaga ttaaacgcat tgaagataag agatccggga
     8641 ggataaacaa cagtattcct gtattcctca cgcacaaacc gggtcagcct ttcaaaatat
     8701 tccttcctga actcatcatt taaccgtaat ttccaccctt cctcaatttt tacatccatc
     8761 ttttgaatga taataaaaat caatataaaa agggccggct tccaatgcca gcccttatct
     8821 gccgtcaatc cgtcacaacc ttaaacttaa ataatctatg acaaacaacc agatgaaatc
     8881 caaaatgtat tttactaact gtaacctcct tacacctaat tacaaaaata ggctccggaa
     8941 agctattttc ccaatatttt caggcaattt tactaaccgg tacctgttaa taaaataata
     9001 cttttcaaca cagaagttgt aattttatcc gaaaacaatg ggaaaggaaa ttgaaaggaa
     9061 attccttgtg aaacccggct tcagggctag tttgccagag gccgtcaata ttgtgcaggg
     9121 ttatttttcc gttgatccgc ttagaaccgt gcggctgcgt atttttaatg aaaaggcatt
     9181 gctgactgta aagagcatgg ccggcagcat gaatcttgcc agatatgaat gggagatcga
     9241 ggttcccatt gccatggccc gtgagatgat ggatatatgc cttcctcaga ggatcactaa
     9301 atcgagatac agcataccat ggggtaatca tacatttgaa gtggatgtat tccatggaaa
     9361 acatgaaggg cttgtcattg cagaaattga gctggacagt gagggcgagc agtttgaaaa
     9421 gcctgattgg ctgggagatg aggtaaccgg caatcctgat tactataatt ccaatcttgt
     9481 ttgaaactct tatttcttaa aaacaaaata tacggcaaga ataagaaaaa caaaagctat
     9541 aagatgattc aggcggaaat gttcgctttt aaaaacgaca agggtaaagg atgtaaaaat
     9601 caggatagag ataacttcct gtatcacctt aagctcaaca agtgagaaag ggcccccgtt
     9661 accttcaaaa ccgatcctgt ttgcaggaac ctgcagtgaa tattcaaaaa gtgcaataaa
     9721 ccagctgaga agaattaccg ccagaagact aaggttggtg aatccaggca atgttttaaa
     9781 tcgcaaatga ccataccatg ccacggtcat aaatgcattc gataatacca gtagtaatat
     9841 ggtgtttaat cctctcattc gttctgcttt tgcagtagat atcagaatat caataccatg
     9901 aacagttaaa gcgtctttga gtaaattctt ttgggatcgc ccggagcata aaaatctttt
     9961 agggttgcct gaagttcata accgtttttc cggtaaaaag cttctgtaga tgcataaagc
    10021 ggcctgcccg aggtttcaat ccacaggatc ctgcccccct ttgccttcac atcttcttca
    10081 acagctttga gaagggaagt gccgtaatga ttgttacgct gatcgccatg cacggcaatc
    10141 cagtacagat cccaggaatg ggttgaaaat ggattttttc catagttggc aaaagccttc
    10201 aagccatctt cagtctttat tttcatccag taatatcccg acttctctgt accatgcgtc
    10261 agcgtttcac tggctatctc cagggcagtc tgaatttcaa attcatagaa gaaatccgtc
    10321 gatcgcagta tttcctcgat tgcctttaat tcctgcctct ctattccacc ggacaacgta
    10381 atcataaaat cttaatagtt aaggtcactg ataatcctgg aaataacttc cgtcattggt
    10441 atgcctgctt caatcgttgc agccaccagc ccgctgtcgg gcgaaataca cgggtttgcg
    10501 ttcacttcaa ttaccagagg attgcctgcc tggtctactc tcagatcaac cctggcatag
    10561 cctcttagcc cgaaaacatt ccagcattta agggctgttt catctatttt ctccctgagc
    10621 atcggatcga gccgggaatg gggaaactcc cttaccgtat ttttatattc aaagctttct
    10681 tcaatccatt tagccctgaa atcaacgatt ttaggcatac cgtgatcaaa attatgaaag
    10741 atcatctcag cagggggcat tacttccgga ccgttcattc cggacaaaat acttatattg
    10801 aattccctcc cgtcgatata ttcctgtatt atccagtgtg aatcattttt cccttcaaag
    10861 tttctttcat atccgggcgt ataagtaaaa accgaatcct tcgttatgcc cagagaacca
    10921 tcttcccaca caggcttgat gatataagat ttgcccggtt ccagtttgct gaactgcgaa
    10981 gggtaataag tcgcaggtat tttaatgcct tctctcacca taatctgatt ggaaagtgcc
    11041 ttgcttgtag tgacaaacag agcttcgagt gaatttcccg tatagggaat ggaatacatg
    11101 ttaagcaggg caggtatgaa ataacataac tcacccttat tgtttatgga ttccaccaga
    11161 ttgaatacga agtcaggcct ctcgccggca agcaagggaa tctctctcat aaaatcatcc
    11221 gtgatgcctt tccggtacga tttcatacca agctttccaa gattttcagc gacatattca
    11281 acctgattca gcacgtcagt ttcatccggc agggcattat tccccggctg attatacaga
    11341 atgcagcata ccttcttctt catttgcgaa atattcagct tttttacgcc ccagcttcca
    11401 tgccagattg ttccccgatt cagtttgcca tccggatatt gaagatccca tcaggtaatc
    11461 ggcgccggca gcttcagatt atttctttcc agggctgaac gcattatcat ttcaatcaac
    11521 gactggaaat ttattccgtt catgtttgca aggataggca ggtcggaatg aacgggatgg
    11581 agacctgcaa ggggattcac ttcaatgaaa cacattttgc cgttaacgtc aaccctgaga
    11641 tctaccctgc ctccatcgac tcctccaatg gcattccatg cactgagtgc aagggtcttg
    11701 cattcttctt catacactcc ttccagggga gtatatttac aaaatatctg ataattttcc
    11761 ttggtttcaa ccgaataggg gaggttatcc ctgcattcta tctccattcc tccaattgca
    11821 actgctttac tgcctgatcc aatgacgcct accgtaaact cctttcccgg cagatactcc
    11881 tcgacaagtg ccggctggtt gaatctcgac aggacaaacc cggccctttc cctgagttcc
    11941 ttccaggacc tcacgaggct tttttccgta atgcctttcc ccgttccctc agaaaccggt
    12001 tttataaaca ggggaaactg cagattgcat tttttaatat catccaggca tgacacaagc
    12061 atacccgggc ttacaggtac gcccgcagct gaaacaatta atcggctgag atatttattg
    12121 agagaaaccc ccaggacaac cgggccgctg aacacatagg ggatcctgta ctggtcaagg
    12181 agtgcaggga caacggattc acggccatct ccatacagtc cttcggcaat attgaagacc
    12241 aggtcccagc gtttatccga agacaacgcg ctcataagct gaaaacaatt gcctatctgc
    12301 tccgtctcaa accccatcct gacaagtgca ttgtcaattg catcaattgt ttcctgcttg
    12361 tcaaattcag cggtatcttc catggagtaa ccacgatcga tataccatga tcgaagatca
    12421 tatgtaattc cgactcgcat cagggaatct ctatttttca ggatattcgc acaggtcgcc
    12481 cttatagttg cgcaacacga tgcgttcgct gttgtgttct actacataat cgggaaggag
    12541 agggatcttg cctcctccgc cgggagcgtc gatgacgaag gttggtacgg catatccgct
    12601 tgtatatccc cttaatcctg caataatctc aaggcccttt tttactgttg tcctgaaatg
    12661 gcccgaaccg ggtattaaat cacactgata cagataatac ggtctcaccc tgatctttag
    12721 aagcctgtgc atcagttcct tcatcacagg aacactgtca ttgattcctt ttaacagaac
    12781 tgtctggctt ccgagcggga aaccattgtc ggccagttta atgcaggcat ttgcgcattc
    12841 ttctgtgatc tcatcgggat gagaaaaatg caggctaata taaagaggat ggtatttttt
    12901 caggatgctt accaatttgt cggttatcct ctgaggcaaa acaacaggga ccctggttcc
    12961 gatacggatt atttcaacat gttctatctt cctgatattg tgaagtatat aatccagaag
    13021 ctcatctgac attgtaagcg gatcaccgcc gcttatcaaa acatccctga cctcacgatg
    13081 cgaggcaata tatgagaacg ctttttcaaa atcctgccgg ccaagcttat caagccgccc
    13141 cactgaatgt gacctggtgc aatacctgca gtaatttgag cagacctgtg taacggtaaa
    13201 aagcaccctg tcgggatatc tgtgaactat gcccttaacg ggagaatagg cccgttcatg
    13261 caaaggatcg gattgttcca tcctgccctc cagaagctcc tcaaccactg gcactacatt
    13321 tctccgcagg ggatgggttg gcttcgaacg atatatcagc gatgcaaaat agggagtgat
    13381 gcgcaatggt aaacgcccct ttagattgtt aattgccagt atctccttct gggtcagctt
    13441 catgatcttc ctgagatctt ccaggcttgt aaaagcattg cgaagctgcc atttccaatc
    13501 attccaagat tctaaagttg ttagggggaa gtagcggttc atgaactccg aactccggtt
    13561 gctaactaac tggtttggga ttacgttttg cgaaaaaatc atggttttcg cgccaggctc
    13621 ttcatcagaa gaacaaccca cgaggcggcc cccgccgagg agggcaccaa cctcattatt
    13681 gttagcattc atatttgtcg atgaaaacga tcacctttta cttttaattg tctagttgta
    13741 tggttttagt taaaaataaa tcgggacgaa aatatatatt ctgcgaatac attgttctcc
    13801 ccggtaatta aaaatattaa cacggttttt aaattatcaa cataaaaaca atcattcaaa
    13861 cattattaac tctattatcc tactgtgtat caacatacta caattcagct gcttgtctct
    13921 tccgtctctc aaatttctac ttctcaagca gatgttagtg taattgccaa ctacctgtaa
    13981 atcaatattc caaatctctc aaagaacaat ttcccaaaaa ctgtcaatta caacatttat
    14041 gccattgact ctgacaatat ttcaattcac aataacaatt tccagttgct tttgttccat
    14101 aaaactgcat tataacgtac tttgataata ttaattattt gaaaggtaaa aaaacgattt
    14161 gcctgcatat gataagcacg gcccggtcac gatgtccgtc agcctggcat ggcagatgcg
    14221 gcggatggga gggaattccc ggaaaggagg actggatgca gatagtgaag ggctaattat
    14281 cgtgtacctt gcgtgaagtt tttctggtct ttttcagatc tgctcttttc cgtttttcac
    14341 cgagacgttt ttccacagaa ctcctggttg gtcgggaagg aattcgttca ggttctgttg
    14401 aaagggattc agcaagaatc gcaaacagcc tctctgaagc gagctccctg ttacgtgcct
    14461 gacttcggcc cgactgtgat ctgacactta ttatacccga agcgcttatc ctgttcttaa
    14521 gcctggaaaa aatcagatcc ttttccgcat ctgacaggaa cggtgaatct ttcacattga
    14581 atcttatttc aacttttgtg ttgaccttat tgacattctg ccctccgggt ccgctgctcc
    14641 tggatgtgat catttcaagt tcactcgcgg gtatccgttt tttgagttca tcgggagtca
    14701 cttcattcag aaatttcaaa cagataactg cctgagccag cctcaatttt aacagttgag
    14761 ccctcaagcc tcacattcgc aaggttttta ttcacgctca cgggcctgcc gctttcctta
    14821 atctgctcca ctgactttgc aggcaaattt atagtggctt ttgagttaac gggtatttca
    14881 attctgcata taatttttcc gttcatcctt tcccagcctg atctgattgt accatacatg
    14941 cttacaaatg atgcttttga ataatcgagt ttttctgttg gatggggctg gataagcagg
    15001 tgcctgtagc cgggtgcaag agtttccagt cctgcagaaa ccctgtacat ccagtcgccg
    15061 atagctccat atgcataatg attgaacgaa ttcatacctg catcctggaa ggtgctgtcg
    15121 ggtttttgtc cgtcccatct ttcccagatt gtggtggcac ccatttttac aggataaagc
    15181 caggatgggt attcttcctg gagcagcaga tcgtatgcaa gatcattata gccattttcg
    15241 gtaagtacgt gacacaggta tattgtgccc aggaatccgg ttgaaagatg gtttcccctg
    15301 cttttgatgt cttcggcaag gaaccgggca gcattcgggc gcacatcatc aggaagcaga
    15361 tcgaacatca gcggcagcac ataggaggtc tgggagttgg ttcctattct tccggccggt
    15421 gtaacatatt catggttaaa aacctttttt attttttcga aaatttcact gtaatatttt
    15481 tcgtcttcct tttttccaag tacgcctgca gccttggata gtaaactgga agaatatgca
    15541 aaataggcag ttgcaatgaa atcatgttcg gtatgtccgt cgggttcggg atgattgtta
    15601 accggaggat gataaaacag ccagtcgcca tagatgcttc cgcctttcca tatcattgac
    15661 tcgccggctt tttttgcgat ataatcaacc caggctttca ttcccggata ctgattcccg
    15721 agcagttgcc tgtcgccgta cacaagatac attgcccatg gtgcgatcac tccgacatca
    15781 ccccatccgg cagacgcaga agcggtaggt gatccctgtt tgtcgaggac atcgggaatc
    15841 acgacgggga tttcccctcc cggtcgctga tcggcagaga catctttaag ccatttggtg
    15901 aaaaagggtg caacattata attaaaagca gcggttctgc agaatgcctg tgcatctccg
    15961 gtccagccga gtcgctcatc gcgctgcggg cagtccgtgg gaacatccac aaaattgccc
    16021 ttctgtcccc atcttatatt atgctgcaac tggttcagga gcggattgga actctcatag
    16081 ctgccggtca tgggcatatc tgaatggata accaccccgg tcaggttttc cggggcaagt
    16141 tcacccggaa aaccggtaac ctcaacatac ctgaatccca taaaggtgaa ccgcggttca
    16201 tatacctcct cgccatcacc cgacaaggta taggtcagct gtgccctggc cgaacggagg
    16261 ttatccgtat aaaactcacc atacttgtca agtatctctg cgtgccgcag tattaccgta
    16321 gtacccctgg ggcccttaac cttcagcctg atccatccaa ccatgttctg acccatatca
    16381 gcaaccaggc ttcccttcgg tgtgcgaaat atcctttcag gctttaattc ttcgatttta
    16441 cgtatttcgg caccttcaga agctatgata ttactcagtg aatagtcgcc tgtcctgacg
    16501 gttgtccaat tgccatcatc aaatccggca aggtcccagc ctgtcagcct cctggttgca
    16561 tcataggttt cgccgttata tatatcattc atcacaattg caccgccaat gtggcctttc
    16621 catgattcat cggtaacaat gaccgattcg gtcccgtcgg aatatcttat atgcaattgc
    16681 ataaggagac ccagatgctt cccgtaaatt gcccagttgt tgccccaggc cagggtgccc
    16741 cggtaccagc cgtcgccaag caatgccccg atcgcattct tgcccggaac cagcattgcc
    16801 gtcacatcgt atacctggta ctggagccgt ttcaggtatg aagtccaacc gggggttaat
    16861 acctggtccc cgactttttt cccgttcaga tgcaattcat aaaatccatg tgaggtaaca
    16921 tatattcgtg ccgacttaac tgacctgtca acggaaaatt ccttccggaa atggggcgat
    16981 ggagaatacc tgttggtatc tgcttccagt tcaatccatt tcgctttcca gcctgccgga
    17041 gatagtaatc ctgtctccca ggatcctgct tcgctccatt tcgactccct gcccttattg
    17101 tcccagacct tcacctgcca gaaatatttt tgtccgggct ttaattcatt tccttcgtaa
    17161 ggtatgaaca cagattcgcc ggaattgatt tttcccgtct gccaggtcat tcccgatccc
    17221 gaaaatttcc ggtcagttgc taccctgatt gagtaggcag tttgcattac attgaagccc
    17281 gtactgctga ttttccagga aagccttgga tgcagggcgt ccacgccaac agggtccttt
    17341 ttgtattcac aggaaagatc gtaaacggtc aggtctctct gtgaataggc cgccagtgaa
    17401 aaaagaaccg gaaataggga agcaagaatt gatttcatga tattatgatc aggttattta
    17461 gttaaagata tgaaatttat ccggcttttc agatttctga aaggtaaaat aattaaattt
    17521 atggttttta aagcgcagac ctaatcaaaa ccatataaat gaatccggtt gcaggaattt
    17581 tactgatagc cctggggagc ataggcgcag ccagtttcta tgtccctttc aagaaagtaa
    17641 aggaatgggc atgggaatcg tactggcttg cccagggcat agcagcgtgg ctcatagcac
    17701 catggctttt tgccataata ttcattcccc ggggggaatt gattcccatc ttgcatgagg
    17761 cgccttcgtc agcaaaattt atggctgtga tgttcggcat cctgtggggt ttcggagggt
    17821 tgaccttcgg gcttagcata cgatatctcg gcgttgcact cggtcaaagc attgccctgg
    17881 gactttgcgc agctttcgga acacttatac ctgctgccgt tgccggacaa aacctattct
    17941 catcccgggc cggaattctc accctcacag gcgtagcgct cacagttgca ggaatagcag
    18001 ttataggtta tgcaggagca ctgaaaagca gggaaatgag tgagaaggat aaacgtgcag
    18061 ccgtcaggga attcgccctt aaaaaaggta tcctgattgc tgtttttgcc ggagtgatga
    18121 gtgcctgttt caatttcggg tttgaggcgg gaaaacctat tgaggctgca gcccttgctc
    18181 acgggacaaa tccgttgttc cagaaaaatc caacacttat attcatactt cttggcggat
    18241 ttatcaccaa tctcgtgtat tgtttatatc ttaacataaa aaaccataca tacagggatt
    18301 atttcaggtt ccccggcaat gtcatcctta ataatatcat gtttaccacc ctggcagggt
    18361 tcctctggtt cctgcagttc cacttcttcg gaatgggtgc cagtaaactg ccccagggca
    18421 tggcagtgtt cggatggagt atcctgatgg cccttaatat tgccataagc aatatctggg
    18481 gaataattct cagggaatgg aaaggatcgg gaacaaaaac aattgcaata cttctgattg
    18541 gtatcgctgt gctgatcctg tcaacttttg tcgttaaact tggttaaacc cagcataatg
    18601 aaaagatttg gctttaaaat gaaactttat ccgggcttta aggaagaata caggaaaaga
    18661 cacgacgaaa tatggcccga gcttgctgaa ctgctcagaa aagaaggaat cgggaactac
    18721 tccatctttt tcgatgaaga aaccaataca ttatttgcct accaggaaca aaaaggcggc
    18781 agctcatcgc aaactctcgg caatacggaa attgtaaaaa aatggtggaa atatatgtcc
    18841 gacataatgg aaacaaatcc tgattactca cctgtcacaa cccctcttga gcaggttttt
    18901 ttcatggaat aatcagatca gatttaaatg gacagatata atattcagaa agtaattact
    18961 ttgaaacaaa ccttcaggta ttaacgacat tggcaataac tatttaataa tcatttatat
    19021 gcaggtttct ttagatcaca gataaataaa tattatgaaa cccacatgat acaaaatcac
    19081 aaacaggcat gaaaataaaa ttcatgtgga tcccatgtga acaacttaat cttcagtaat
    19141 atataaattt attttcatat gaaaaaagaa cttatcagga agtcatatca acttgcaaag
    19201 gaacaatatg ctgaacttgg tgtggatact gaaaaagcct tgtcggaaat ggataagctg
    19261 gtaatcagcc ttcactgctg gcagacggat gatgttggcg ggtttgaaag ggaaggtgcg
    19321 gagctgggtg gcgggggcat acaggcaacc ggcaactttc ccggcaaagc caggacaatc
    19381 ggacagatga gagatgatct tgataaggtg atgtctcttc tgccggggaa gcagagactg
    19441 aatcttcatg cgatttacgg ggagtttgaa gggaagaaag ttgacaggga ccagatcgaa
    19501 ttaaagcatt tccagggatg gatcgactgg gcaaaggaaa gaagcatggg ccttgatttt
    19561 aactgtacct gtttttcaca tcccctttcg gatgatggtt ttaccctgtc gagcaaaaat
    19621 cccaaaataa gaaagttctg gattgaacat acaaaaagat gcagggcaat agcagctgaa
    19681 atgggcaggc agctcgggac cccatcggta cacaatatct ggataccgga tggttcaaaa
    19741 gatactccgg tcgacaggta cacactgcga aaaaatctca agaaatctct cgatgagatc
    19801 ttcgagatca gatatcccgt taaatacctg aaggatgcaa ttgaaagcaa gctgtttgga
    19861 attggttcgg aatcgatggt agtcggatcg cacgatttct acctgggcta tgccataaag
    19921 aacaataagc tcatcaccct tgataacggc cattttcacc cgaccgaaca ggttggcgac
    19981 aagatctcat ccatactcct ctttgttgac gagatacttc tccaccttac ccggggcgta
    20041 aggtgggaca gcgaccatgt tgtgacactc aatgatgacc tgatgctcat agctcatgaa
    20101 atagtcaggg caaaagcatt gggcagaata aatgtgggcc tcgacttttt cgatgcaagc
    20161 cttaacaggg taggtgccta tgttgttgga accaggtcgg tccagctggc cttcctgagt
    20221 gcgttcctcg aacctaccgc aacactggtc aaatatgagg aagacggtaa aacctttgag
    20281 aggctttctc ttcttgaact tatgaaaacc atgccgctag gcgccgtttg ggattactac
    20341 tgcatgataa atgacgtccc ggcgggaccg gattatatcg atgagatcag gaaatatgaa
    20401 aaaaatgttt tgctgaaaag ataaactgag tcgaacataa aaaccctttt tcgggagatc
    20461 tattagtcat cttatgaaag aaaaggttat tgcagtattt gatataggaa aaagcaacaa
    20521 aaagctgctt ttatttgatt ataatctcag gcttgtttca gaaaatgaga ccagtttccc
    20581 tgagcaggtt gacgatgacg ggtttgcatg cgataacatc ggtgccattg aaaaatgggt
    20641 cagggattcg gtaagcgccc tctcgctttc cgataaatat gatctcactg ccatgaactt
    20701 tgcaacttat ggtgcaacca ttgcctatct tgacaaagat ggaaaacgga tcacccccat
    20761 atacaattac ctcaaacctg ttgatgaaaa attacctgag aggcttttca gaaggtacgg
    20821 aggacaggat gaattttgcc gcaggaccgc ttcacctgcc cttgggatgc ttaactccgg
    20881 catacagata ttctggctga agtcggaaaa accggatatt ttttcgaaag ttgatcagat
    20941 cctccatttc ccgcaatata tctcatacct gtttacggga aaaatatact ccgaacatac
    21001 ctcagtgggc tgccatacga gcatgtggga tttcgacaac atgaaatatc atccgtggct
    21061 ctcggatcat ggcatcaaac tgcctgaacc agtatctgta atgacctgta acgagattga
    21121 aagcgaaggt aaaaaaatca tgacaggaat aggaattcac gacagttcag cttcccttgc
    21181 gccatatttc accggaaaac gagggaactt cctgctcatg tccacaggaa cctggtgcat
    21241 caacatgaat ccgttcaatc ctgaaaaact taccacaatt caacttgaca atgactgcct
    21301 gtgctacatg agtgtcagta tgcaacccgt aaaatcatcc aggctgttcc ttggcaatat
    21361 gcatgataat ggcgttcgga ttctttccga tcatttcagg acctcaggca attattacaa
    21421 ggatctgaaa tatgacaatg atctgataca aatgctgaaa ggcaaataca gcggtgacag
    21481 gaaagtattt ttcagctcgg aatcagggtc acgtgaattc agggacaata tagatctgtt
    21541 cgagttcagg acatttgaag aaggttacca tcagcttatg attgaactga gcgaactgac
    21601 catagcttct gttgaactga tcctggctga aaatgatgct accgaaaaca tatacattac
    21661 cggcggattt tccaggaaca atattttcct taggctcatg gcccaggcct ttccttcaaa
    21721 aagagtattt acatcagaag taaagaacgg gacggcgctg ggcgccgcac ttgttatact
    21781 gcaggctctg gggtaccctc ttccggaact caaccttgga ctgacggaaa tcagcagctg
    21841 acctcacaac cacattccgc gaatattcta ttatcctttc tgccagcatt cttcggattg
    21901 ccgcctccgg catttccgta cagcatatct tgttgattcc gggaaatgtt cataattact
    21961 ttttatgtta ataataaata ttttttatat ttaaggatca cttttcaaac ctaaaaaccc
    22021 tgataatgga caaggatgaa cagattatcc ttaagattct gatatacata atcatttttc
    22081 tgctgttcgg aattataatc atcacatgaa agataaacac atgttccgga tcaatcagcc
    22141 cgttcccctg gctctttgtg cctccacatt tttcctgatg tcttgcactg attccgccga
    22201 gaaaagagct ttgccaaaca ttgtttttat cctggccgat gatatgggct acggcgacct
    22261 tagctgttac ggtcagcaaa aattctccac tcccaatatc gaccgcctgg ccagccaggg
    22321 cattctgttc acgcaacatt acgctggatg cacagtaagc gctccgtccc gttcaagcct
    22381 tatgaccggc ctgcatacag gccacacacc aataagagga aacaaggaat gggatcctga
    22441 aggacaatgg ccgctcccgg cagcatctgt tacaatagcg gaaatgctga aatcgtgcgg
    22501 ctacactaca ggagctttcg gtaaatgggg actgggattc attggtacgg agggagatcc
    22561 caactctcag ggatttgatg agttttttgg ttataactgt cagagacttg ctcataatta
    22621 ctttcccggt cacctgtggc ataaccggga aaaaatcatc ctccacgaca atgattcagg
    22681 caggaccgga tcatacggac ctgacctcat tcacagggcc gcactggaat ttattaaagc
    22741 taacagggaa aaaccttttt ttcttttcta ccctacaatc attcctcatg cggaacttta
    22801 tgccaaagat caatacatgg cacagttcag gggaaatttc gaacccgaga aagtgtttaa
    22861 aggtactgac agcggcccgg gcttccgcaa aggggcatat ggctcccagc cggaatcaca
    22921 tgcagcattc gcgggaatga taaaacagct cgatgattat gttggcgaaa ttatagcttc
    22981 aatcagcgaa ctcgggcttg aaaaaaatac catcgttatc ttttcgtccg ataacgggcc
    23041 gcacctggaa gggggagccg atcctgatta ttttaatagc aacgggaagt tcaaaggtta
    23101 taaaagggat ctgtatgaag gcggaataag ggtcccgttt atagtgagat ggcctggcaa
    23161 agtgaaacag ggccgcattt cagatcatgt ttcggccttc tgggatataa tgccaacctt
    23221 tgcagaagca gccggtattg atgctccaga agaaactgac ggaatttcat tcctgccgga
    23281 gcttcttggc agaaagcaaa aagatcacga attcctctac tgggaatttc acgaacaggg
    23341 tggccggata gccgtcagaa cagggaactg gaaagcagtt aaattcaaca ctgaattgac
    23401 tgacaccgga caaattgaat tatataactt atctgttgat ccgggtgaaa caaatgatgt
    23461 tgccggatca aaccccgaag tggtaaagga aatgctccgt ataatgaaac gggaacatgt
    23521 gccgtcagat atctttccca tggcagctga aagatgataa gttgagaacc ggacagacaa
    23581 tcggatgcag accattcaac gaattattaa acctgatcac tgaaaaaatg cttccatgag
    23641 actttgtact gaaaagaaga ttctgctgcc agtgctgatg gcttctgtac tgccactctc
    23701 ctcatcgtgc cggaaggccg aaagtcccaa cattatttta atttatgcag acgatctggg
    23761 ttacggggat gtcagctgtt acggtgcaaa gagcatcctg acgccaaaca ttgacaggat
    23821 tgccaggcag gggctgcaat tcagcaatgc gcactgcaca tcagccacaa gtaccccatc
    23881 gcgatattct ctgcttaccg gagagtatgc ctggagaaga cccggtaccg gaatagctcc
    23941 gggtgacgct ccttccgtaa taaaagcagg cagattcacc attgcatcac ttttgaaaag
    24001 cgccggttac acgacagccg taatagggaa atggcatctc ggacttggac ctgaaggcgg
    24061 acctgactgg aacggtgaga taaggcccgg gccaatggac ctgggttttg actataattt
    24121 tatcatgccc gctacaggtg accgcgttcc atgtgtcttt gtcgagaaca ggaatgtgct
    24181 tggtcttgat ccggatgatc cgattcgcgt cagttttaag gaaaaaatag gcagtctgcc
    24241 gaccggaagg gaaaatcctg agcttctaaa aatgcatcca agtcatggtc atgatatgac
    24301 aatcatcaat ggaataagca ggataggata tatgacagga gggacttcgg cactctggac
    24361 cgacgaagat attgcggatg tgattacgga aaaggccgtc agatatataa aggacaacaa
    24421 gaataggcca ttctttttat attttgccac aaatgatatc cacgttccga gagttccgca
    24481 ccccaggttt gcaggaaaga gcggtatggg tccgagggga gatgttatcc tgcagtttga
    24541 ctggtcggta ggtgaaatca tcaggactgt tgaagagctg gatctgtcgc gcaaaaccct
    24601 gatcattgtg acaagtgaca acggccccgt ggtagatgac ggataccgcg accaggctgt
    24661 tgaactgctt ggcgatcaca aacctgccgg cccgcttcgc ggaggaaaat acagcgcttt
    24721 tgacggcggg accaggatca tgtttattgc cagctggaaa ggaaaaatca aacccgggaa
    24781 gtcagatgca ctgttcagcc agatagacct catgtcgtcg tttgcatcac ttaccggaca
    24841 aaccattccg gcaggggaag gacccgacag ctataacaac cttccggtcc tcctgggaaa
    24901 atcccggaaa gacagaagct ggctggttga acagtcgctc aataaccggc tttcgatcat
    24961 taagggtgac tggaaatata ttgaacccgg accaggaatt agaatcatgc ccgaaaccgg
    25021 aaccgaaacc ggcaacgatc cggaacctca gctttacaac ctgcgcaggg atatcggtga
    25081 gaaaaataat gttgcccggg aaaaccagtc aattgttaat gaattaacag aattgttgca
    25141 agaaatcagg aattctgcag aataacctaa ataatgcata atgaaaatta atctgaagcc
    25201 tgcactgggg ataggcgtga tagcctccgt tcctctcgtt tcctgtcaga actcaggaaa
    25261 acaggaaaca tcaaaaccaa acattctgat gatccttctc gacgattacg ggtacagcga
    25321 tgtcggatgc tatggaagca aattctatga gaccccgaat attgacaggc tggccagcca
    25381 gggaattatg tttaccgatg catatgcttc atgtcccgtc tcctcaccta cgagagggtc
    25441 tatactgacc ggcaagtatc cggtaaacac aggtataacg gaatggatac ctggaaggca
    25501 ggccactgat tacggtgctc catatgacaa gcttctgccg ctgcctttca aacttttcct
    25561 tgatcctgag gaagttaccg tggctgaaat gcttaaaacc aaaggttaca caacaatgat
    25621 ttccgggaaa tggcacctgg gcgaagatcc ggcctactgg cccgaaaacc agggctttga
    25681 tgtaaacaaa ggtggatata aggcaggtca gccatttgtc accgccacgg ccaacggata
    25741 tttctctccg tatggaaacc cgcggcttga caacggacct ccgggtgaat atcttaccga
    25801 caggcagacg gatgaagcca tcagattcat tgaaaacaac agggaaaagc cgttttttgt
    25861 cctcctttca tattatgccg tacacacacc tttgcaggca aaggaacagc atataaaaaa
    25921 gtttactgaa aaagccgatt cgatgggact gtcatccatc tctcccttta ccagggacaa
    25981 agactggata aaaccctcca tgccggacga cttcagggaa agaatcattc agagcaatcc
    26041 ggtttatgct gcaatgatat ggagtgtgga tgagaatata ggccgtgtgc tggccaggct
    26101 tgaggaactc ggtattgacg ggaataccgt tgtgatcgtt acctctgaca atggaggact
    26161 tgcgacttcg attggctccc caacatgcaa tgctcctttg agagccggaa aaggatggct
    26221 ttatgagggg ggaatacggg tacctctcat cgtaagatat ccggggctga aatctccggc
    26281 taaagtagtt gattatccgg tttcatccgt tgatttttat cccacctttg cggaaattgc
    26341 cggctatgac tgcagcaatg agaaaattga cggaatcagc ttcctttcat cgctttcggg
    26401 caggaatcct gaagacaggc ctttattctg gcactatccg cactacaata accgtggtgt
    26461 tgaaccaggg agcgcaatca ggctgggcaa atataaactt atagacaatt ttgagagaga
    26521 gaggcaggaa ctgtatgatc ttgaaaacga catatcggaa acaaccgata tttcagcttc
    26581 aaaccctgaa aagaccaggg aactgttcac tttgctgaat gactggaggg ccctgacggg
    26641 tgcaaaaatg atgcttccaa accccgcatg gaaaaaggag tgatcccggc ttgtttttat
    26701 tttgattaca aaaaccatat gccatgttaa aacttaattc cgtccttact gccggggcag
    26761 taacactggc aggcttccag gcccctgccc agaagcctgc agcactaaag gcagccactc
    26821 ccaacattat tattatcctg atggatgata tgggatatgg cgatatcggc acgaccgggg
    26881 caaatcaata tgacacaccc aatctggacc ggctggcaag ccagggaatg cagttcacct
    26941 ggttttacag tccgcaggca gtaagcagcg cttccagggc gggactgttg acaggatgct
    27001 accccaatcg cgttggaatt accggggcac ttatgccatg gtcaaccatt gggatcaatt
    27061 ccgatgaaac aactattgcc gaggttctca aaacaagggg atatcatact gcggcttttg
    27121 gcaaatggca tgtgggccac cagagggagt tccttcccct ccagcacgga ttcgatgaat
    27181 attacgggct gccatattca aatgacatgt ggcctgtcga ttttgacggg gtcccgattc
    27241 atctcaggga tactaccagc agacttatga gatatccggt tctcccgctg atagaaggaa
    27301 atgaaaaaat aggagagatc actgatcttt ccggtcagga taaactgact gccgaatata
    27361 ccaggcgggc tgtggaattc attgaaaggc acaggaaaga acctttcttc ctttatctgc
    27421 ctcattcaat ggtgcatgta cccctcggtg tatctgaaaa attcagggga aaaagcaggc
    27481 aggggctgta cggcgatgtc atgatggaag tggactggtc ggttggggag attgtaaaag
    27541 cggtcgacaa aaacggcctt gcaaaaaaga cactgataat ttttacgagc gataacggac
    27601 cctggcttaa ttttggaaat cacgccggta ctacgggcgg attacgcgaa ggaaagggaa
    27661 caacatggga aggcggccag agggtgccct gtataatgag atggacaggt gtcatcccgg
    27721 caggggaaat ctgtaaccgc cttgccagtt caatcgattt tctgcccacc gttgccgcca
    27781 taaccggggc aaagcttccc gggaagaaaa tcgatggcgt caatatactc ccgttaatgc
    27841 ttggcgataa aaataccaca cctcgccatg aattgtatta ttattacaag accaacagcc
    27901 tggaagccgt tcagcgcgac tactggaaac ttgttctgcc tcacaggaac aatcagtcat
    27961 accgttatgt ggatccgggg aaagatggct ggccgggacc ctatggctca gaaacaatct
    28021 attcacctga actatatgat ctccggcgcg atcccggtga atggtatgac gttgccgccc
    28081 tgtatcctga aaaggtaaaa gagctccagg atcttgcagg ggaagcaagg cgcgacctgg
    28141 gtgatgaaat aacgggcgat ccgggaaccg gacgccggca gcccggaaca attaaagaat
    28201 aaataaagta ttattgtcat aataaaatca ttcaatatga aattttacct cggatacgcc
    28261 gcactggctg gtatgggtgc ggtccttgga caggattcag ccggcccttc accggccaac
    28321 gaaaggccca acataatcta tattatgtcg gacgatcacg cttaccaggc aataagcgca
    28381 tatgaaagca tccttaagga ttatgctcca acgcctaaca tcgacaggat cgccagagac
    28441 ggaatgcgtt ttgaccgatg tcttgttacc aattccttgt caggtccatg cagggctgtt
    28501 atactgacag ggaagtacag ccatgtaaac ggatttatca ctaacgatga tcagaaaccg
    28561 tttgacggat cacagcaaac ctttccaaag ctgcttcaga aggccggata caacactgcc
    28621 atgatcggca aatggcacct ggaatctgaa ccaaccggat ttgatcattg ggaaatcctc
    28681 cccggacagg gggtttacta taatccggat ttcattacca gggaaggcag gcatacagag
    28741 aagggttatg taacggatct gatcggaaaa atgtctcaat tggcttgata atgcaaaaaa
    28801 ctccggaaaa cctttcatgc ttatgatgca tcacaaggcg cctcaccggg aatggcagcc
    28861 cggtcctgat gaactgtccc tttacaagga tgtaattttc ccggagcctg aaacactttt
    28921 tgatgactac gccggccgcg gtaccgccga gaaaacacag gacatgacaa tctctaaaac
    28981 aatgcgtatt gaggaggacc ttaaaatgta caaggatcgg tcgaagatgc ggcggaccgg
    29041 gctcgaccgc atggattccc tgcagatggc agaatggaac aaggtatatg atccgattat
    29101 ctccagattc tatgaatcgg acctttcagg caacgatctt gtgagattca aataccagag
    29161 gtacctgcaa gactatctcg cctgcattgc cgcagttgat aaaagtgtgg gagaaatact
    29221 gaactatctt gaaaaaaacg gactcgataa aaacacagtg gtaatatatg cttcagacca
    29281 gggtttctat ctgggagagc atggctggtt cgacaagaga ttcatgttcg aggaatcata
    29341 tcgcactcca ctggttatca gatggccggg agtcatcaga cccggaagtg taaacaacga
    29401 catggtatcg aatattgacc ttccggagac tttcctggaa atggccggac tggacgttcc
    29461 ttccgatatg cagggaagaa gcatggttcc tgttttaaag ggaaaaaccc cttcagactg
    29521 gagaaaagag cactattatc attattatga actccccggg ccacatagtg tcaaacgtca
    29581 ctacggaata agcaccgacc gctacaaact gatccattat tactatgata ttgatgaatg
    29641 ggagctttac gacaggaaag cagatccatt ggagatgaaa aacgtttaca atgacccgaa
    29701 atacgccaaa atcaggaaag atctccataa gcggctcacc tcgcttatga aaaagttcgg
    29761 ggacagtgat gagcttgcaa ggtcattcct accaaactga ccgcctgcgc atgaaaatta
    29821 catttgaaaa tattctcctc attggttcag ttctgttctt aataagcata atcgcaggca
    29881 aaacttcatt tcggctgggt attccaaccc ttatcttttt ccttatcatt ggaatgctgg
    29941 cgggaactga cggaatcgga aagattgaat tcaacaaccc cagcatagca cagttcatcg
    30001 ggatagtggc cctgaatttc atactgttct caggtggttt tgaaactgac tggaaatcag
    30061 tgaaacctgt ctttggcatg tcattttccc tgtccatact gggagtaatt cttaccgctg
    30121 tttttctggg tatttttaca tggctggtaa ctgactttac actgttcgaa ggattgcttc
    30181 tcggatcaat tgtgtcatct accgatgcag cagcggtatt ttcaatactc cgctcaaaaa
    30241 acgttgcact aaaaggacat atcaggccaa tgctagaatt tgaaagcggc agtaacgacc
    30301 ctatggccta catactgaca attatgttcc tgggcatgat aactggtgaa gtcagttcat
    30361 tctcgctggg cataaaattg tttttgatgc agctgctgat cggaggaggc tgcggcttcc
    30421 ttttcggata tttaagcaaa caagtgatta acaagatcaa actcgattat gatggacttt
    30481 atcctgttct gtcaattact ctgatgttcc tgacctattc cctgacgaat ctggccgggg
    30541 gaaacggatt cctggcagtt tacctgtcgg cactttatct cggaaaccag aacctgctgc
    30601 acaaaaggac aataatgaag atgtttgacg gccttgcctg gttgatgcag atcgtgctgt
    30661 tccttgccct gggcatgctg gtgtttccaa cccacatatt accggtggtt ggcgtagggc
    30721 ttgcaatttc agccttcctg atgattgtcg ccaggccact tgcttgtttc atttgccttg
    30781 ctccgttcaa aataaaaaca agggtaaaat ggtttatttc atttgtcggg ttgcgcgggg
    30841 cggtcccgat tgttttcgcg acattcccgc tcatggcagg aatagataag gccggaatga
    30901 tcttcaacat agtgtttttc atctctcttt cttccgtact gattcagggc acctcaatac
    30961 ccagggttgc caaatggctg cacgttgatc tccccgtaaa tgtcaaatcc gtaacacccg
    31021 ctgatgtact gctcgcggaa tcgttaaatt cacaaatggt ggaactaacc atcgcatcag
    31081 gattcatggc tgtagggaaa aaaattgttg atctgcattt tcccccaaac gtcagaatct
    31141 cattgattaa aaagaaggga atatatgtca tacccgacgg caacactgta atcgactcgg
    31201 gtgatgtttt atttattata ggaacaaacc gggaggcatt ggattccgta acggaaatga
    31261 tctcagagag aagagcttag actattgtat ttaaattttt aatttatgtt ggcttatgaa
    31321 taaatcatcg atcccaataa ttttctttct gttattgact tacatatttg ttgaagcaca
    31381 ggaattcact attccattgt acaatggtcc ggttccgaat tcgaaaaaca ccgggcaggc
    31441 ggaaataatt gagaaaaacg aaattatcag catccgaaat gttcaggtgc ctgaaattgc
    31501 cgtgtatctt ccagccagga agtttgccac cggtcaggca gtaataattt gtcccggagg
    31561 aggatatcat atactttgct atgattatga aggtaccgat attgcccgct acttcaactc
    31621 catcggaata gcaggtattg ttcttaaata caggctgccc atatacggaa ataccctgga
    31681 gccgcacaag gccccgctta tggatgccca gagggcaatc cgtctggttc gcagcaattc
    31741 gtccgcctgg aacatcgatc cggggaaaat aggaataatg ggattttcgg caggcggcca
    31801 cctggcgtca tgcaccggaa cacatttcga ctacggaaga aatgactcag acgatccggt
    31861 cgaaagaata agctgccggc ccgatttcat gatccttgtg tacccggtaa taacattctc
    31921 tgatcctgtt tcgcatcggg gatcgcggga agcattgctt ggaaagaacg ccgaccctga
    31981 acttgtaaaa tattattcat cagaattgca ggttcagaaa gatacacctc aggctttcct
    32041 cgttcacgga gataatgatt cagtcgtccc agtcagtcat tccctcctga tgtatcaggc
    32101 ccttcacaac aggaaaatcc cggcagaact ccatataatc accgaaggtg aacatggttt
    32161 cggacttgcg gttgacaatg accaccccgc agcatggaca aacaatcttg agcagtggct
    32221 gaaatggctg aacaggaaca ggaactagaa ctgaagcatg caggggtgtc ttgaccggct
    32281 gatcagaagt acttcaccaa acagcaattc ttgaatttct ttcccgaacc gcaggggcac
    32341 ggatcgtttc ttcccggcaa ttcaggcctc acaacctgac tttccctgtt ccttatccac
    32401 tgcatcacgt ttgccggagg gcgtttgcct ttcagttcgc ctgccatgaa ctccatgtat
    32461 ggttcaacat gcttgaaaaa tctcctgtat cccctgcaaa ggtaattcag cccgggctta
    32521 ccgtcggggg tcagtatgaa ccggtgtttg ggacattccc catgacaggc atacctcact
    32581 tcgcatctaa ggcaatattc cggaagtgaa tttcgtttgc cgatgccaaa atcaaattgt
    32641 ttctgtgacc tgacaagatc aatcagtccg gtgtcattaa tatttccaag aaaatattcc
    32701 ggatatacaa aatgatcgca tgagaacaga tcgccattat gttccatcac aagtgcatca
    32761 ccgcatgtat cgttgaaaac gcacaatccg ggattctcgc cgacgtaatt ggcaagtgtt
    32821 gcatcaaaaa tctgaacaaa atatcgtgcg acatcatttc tgacccattc atcgaatatg
    32881 gcaatcagga aatcgccaaa atccccggca ccgaccgacc attcagttac ccttgcaaca
    32941 ccggcaaaac cgggcgaaac caggttcagt ccgtcctccc caatatcatc acagcttctt
    33001 tcggccaccg ggaggaactg gatgaatccg ctgccgatcc ttttaaggaa cctgtatgtt
    33061 tccagagcga aatgaacatt ataactgttt accacactga gtgtgttgaa ttccacctta
    33121 tgcctgtgca gcaattcaat tcccttcatt acccttttaa aagtcggtct gcctgaaaag
    33181 gttttccggt aatggtcatg attatgttct tcaccatcta ttgagattcc taccagtatt
    33241 ttgttatcat gaaaaaaact gcaccagtca tcatcaagcc ttgttccgtt agtctgaaaa
    33301 gcgttctcaa tttttttccc gttacggtat ttttcctgta gttctatggc tttcctgaaa
    33361 aaatccaatc ccatgagtgt cggttctccg ccctgccatg tgaaagtaac aacaggtata
    33421 tcatgagctt caatgaattg cctggtgaaa ttctccagga gttcttcgct catatggaaa
    33481 ttcctggtgt ccctgtacag cttccttttc tcaagataat agcaataggt acagttcagg
    33541 ttacaggaag gacctgccgg tttaagcatc acatgaaagg cattgggccg gcgaagctta
    33601 agtgcatctt taaaaaacag gtttgatttc atggaataat attcagatac aaaaataata
    33661 ttatagccag gataaaaaag aattgacaaa gcaatggatt gcaggctaaa aatcataaat
    33721 tggaaaaata ttgtcaaccg gaataaaaaa cacctcctat gaaaagaaga cgatttcttg
    33781 aaacagcttc aatgggcgct gcagcagcaa tgtcatcaat ctggcttccg cccgttactg
    33841 gagaaagcaa gctgaagatc ggcctgatag gtgcaggctg gtatggaatg gtaataacaa
    33901 aggcggcact gaaaaccggc gagacagaag tcctgggagt atgtgatgtg gactccgcac
    33961 atctggaagg cagtgctgat gaaatcgaaa agatccaggg attcaggccc cggacattca
    34021 agtattatga agaactcctg gaattgagag gattgcaggc aatatttatc ggaacccctc
    34081 cgcactggca cgcattacag tttatcgcag cctgcgaaaa aggctttgac atatattgcg
    34141 aaaaaccgct tgcctacgac gtgcaggaag gtcttgccat ggtgactgcg gccaaaaaag
    34201 cagggaacat tgtacaaatt ggctttcagc ggcggcagag cgaggcgttc aggaaaacaa
    34261 aggaactgat agcttcaggg aaaataggca aggtttatca gataaatgcc cagatcaact
    34321 atatacccga tcccgtcgat acaacaattc agcctcctcc gccatcactg gattgggatg
    34381 cctggtgcgg accggccccg aaacttgaat acaggccaag tatcgggcat atgtcctgga
    34441 ggcttgaaaa ggagtacgga aacggtcatc ttgtcgactg gggcattcat catatcgata
    34501 taatcagata tataatggga gaggggatgc ctgctgaatt ctactcatca ggagaaatca
    34561 atgtattaaa gggccggata accacccccg acacacttgt tgcaagaatg acattcagta
    34621 aagctcctgt catctggcag cacagactct ggggcacagg cgatgttatc agggaattca
    34681 ataacgggat catgttttac ggagacaggg gaaccctttt cgctgaggac tcgaaaatca
    34741 tactcttcga caacaacaac ctgcgggagg aaattcctgc agcaacaccc ggcatgcagg
    34801 aaaagcatgt agccaatttc atagaggcgg tcaggacgag aaacatgaat ctgctcacct
    34861 gtaccacgga agaagccttt atgtcaaccg ctgcggttca gctggccatg atctcctgtt
    34921 ataccgactc acttgtcagg tgggatgtca attcagcctc aattaccggg aataccgaag
    34981 catcaagact gttgagaagg gaatatcgca agggatatac acatccgtaa gggctacctg
    35041 tgccggtcgg aacatctgtg ccggccagac atgaaataag caacaggaac ggtaaaagaa
    35101 atgagagaaa aactacctgc gcattatata tgaaatccgg ctgttcctca gggatacaca
    35161 tccggtaggg atccgaggta ctccgcaagt ttcagaagat cgtcacgtga tccgaaactg
    35221 attttttcat ccttaatgaa tttcatgatc tcctgcttat gccccggggc cgatcggatc
    35281 gcattcttgc gagtgaacaa aacaaaaggt cgttcacctg atgagaaata atactcatgt
    35341 gttttctgaa aaacccagtc ctcctccgga cgcagttcag taatgtttcc tgacgtggcc
    35401 agataggtat gcgaatcaac cgccgcactt cgggctgttg tcccaaaagc gccggtcttc
    35461 cgtacctctt tgagtgcaat caattgcttt aaatacaccc tgggtgtgcc gcttctgatt
    35521 aattcgagat atccgttgaa gtaataaaat gtatcaatgc ccagggttat cagcttcaga
    35581 tcttttttgt tcacgatcga aagggtatct ctgtctttta tgaattccat gttaccaagc
    35641 aaaagattat agttgaaacg atcctttgtt acatttccgt tcttcataac agccaaacct
    35701 tcagtaaact cgggataacg atatcttatg gcaggaggaa aaacactttt tataagagtt
    35761 cctgcattta cgacaatcat atcgctttcc tgggaagaaa gctggatggc aaacagaaag
    35821 atcgcgaaaa aaaccagaat tattctattc attttcattt gttattaaaa tcacacaccc
    35881 cggagcataa cctgcatggc cttaaaaccc tgctgcaaca aggtacggag ttttcagttc
    35941 agatgctgaa cacatcagca aattcatata gcccgtaatc cgagcatatg ccatggaatt
    36001 cattgagcag ctcgggactg aatggcactc ccctgtcttt ccgggatatc caggtttcat
    36061 actccttttc tccggccgta tagatacgtg aacgcccggg cattttcctt gagttcctca
    36121 gctcacgcaa tatgtcaccc gctgttctac ggaaatcatc tggctcggtg aatgcactga
    36181 catccaaggc aataaaaaaa tgacccagtg agtaagggac tttcttgctt ccggaaattc
    36241 ctgaaatcat ttttaaaaaa gcaccctgct gcagggccga tgaaagtatt tccacaacag
    36301 ttgcgtatcc gtatcctttg tatccgcctg tttcctcacc gatccctccc agaggcagga
    36361 gggcggcagt tccgtcattc aattcttcca gaatttgctc cgggtcagtc tcagtcattc
    36421 ctctgtcgtt aataaccagt cccctgcgta atttctttcc ttccctcgca tataattcca
    36481 ctttccccct ctgcgtgatt gaagtggcac aatcgagcag aaaaggaaaa ggctcatcag
    36541 tgggaatccc aaatgttaac ggattggtcc ccagcatatt ttcgacacca aaggtgggag
    36601 caactgaagg ccgggcattg gtgcccgtta ttcctatcat attctcacgg acagccataa
    36661 ggggataata accggcaaaa ccataatgcg acgaatttct tacggctacc attcccattc
    36721 cataatttcc tgcttttttt atggccattt ccattgccat ccttgcaatt acatggccca
    36781 ttccgttatg tccgtcaata actgccgttg tatatgtttc cctgatgatt tccggttttg
    36841 taacagggtt cagaatgccg tccctgatgc gatcgagata tacacttttg agtctgttga
    36901 caccatgcga atcaaaaccc agtttgtcag cctggatcag aacatctgcc actatggcgg
    36961 catctgctgc cggaataccg gccctgatca ttgctttaac catgaagtct tccagtatct
    37021 caaattgtat atactttatt ttcatttatt aatgattgtt aggagattag cataaagcat
    37081 cagcagtgtc tcagatgaca tcatgatcat tttccaatgc aaaagttctt aaatatattt
    37141 ccaagtatct catcagtcgt tatttcacct gtaatctcac ccagataatg gattgcatgc
    37201 ctgatgtcaa tggcaataag gtcttcggga agcttgcttt caagtcctgt tattattctt
    37261 ccgatgcacc cggaaatatt ctgcagggct tcataatgcc ttatatttga tataattaca
    37321 tcttcacccg aaaaataatt tgcacctgtt atttccgaca ggtaatcctt aagcttatcg
    37381 attccggtac catattttgc tgaaataaac aacaggtttt cgtctatttc tgtccttatt
    37441 tcgcgcgcaa gcctattaat ccgcgaagga gacgcctcat cagccttgtt taccaatatg
    37501 ataaacctgc actcaggatc attgatcatt tttctgattt catctgccct tttgttaatg
    37561 tctgagcttc tttctcctga ttcatcaacc atcatgacaa ctgaggccct ggcaataatg
    37621 gcaagggttt tcctgattcc cattccttcc accacatctg aagtatcacg taaacccgca
    37681 gtatctataa acctataaat caatccattt atatttacag tgtcttctat tgaatccctt
    37741 gttgttccgg ggatatcagt gacaatggcc ttttcttcat taagcaggaa gttcagcagt
    37801 gttgattttc ccgagtttgg ttttccgaca attgccacag ggattccatt ttttattgcg
    37861 tttcccattc taaaagaggc agagagtctg tcagttagcg ctttagtctg accaatcagc
    37921 gctaataact gattcctgtc agcaaactcc acatcctcct ctccgaaatc aagttcaagt
    37981 tctatcaggg aggcaaattg caaaaattcc gctctcagct tgcttatttc atctgaaaaa
    38041 cctcccctta actggttaat tgcaatccgg tgagccgact tagaagagga tgcaaccaga
    38101 tcagcaacag cttcagcctg cgaaagatcc attttaccgt ttaggaaggc cctttgcgtg
    38161 aattcacccg gacgggcact tgcagcaccg tttcttatta gaagatccag gatccgtgaa
    38221 atgatatatg acgaaccatg acaggaaatc tcagccgtat cctcgccggt ataggaatga
    38281 ggagccctga aaagtgagac aagaacttca tctatcactt cacctccgtc gcggatttcc
    38341 ccatacacaa tggaatatcc tttccggccg gccgtcactg agttcttatc cgcgggataa
    38401 aagaccctgt ccactatttt gagtgcatcc ctgccgctta tcctgatcat tgaaactgcc
    38461 ccgccatatg ctgttgccgg agcacaaatt gtctcttccc ttcctggcat ttaaacgacg
    38521 taaaaactgc aaatttagaa aactttacag cttcagtaca gatgctgaat ataaaatcac
    38581 tgaatttcgt atgtccgtaa atattttaag aactatcttt aaagggaata tgcaaataca
    38641 aaaaaggaaa tccagaaatg aaagaaaaaa tcatttttcc aaccttattt ttatttctta
    38701 tgtttaatca agcttgtcag aaaatgaaaa atgacagatc agaaatcaaa tcggactata
    38761 tcagtgcgac ttttctgctg ccggatgctg agaacggtta ttacagaggt accaggttcg
    38821 actggtcagg cgtaatatcg gacctttcct gctgcggaca cacatatttc ggaaaatggt
    38881 tcgataatta cagcccaaca cttcacgatg ccataatggg tccggtcgag gaattcgcgc
    38941 cccttggata tgacagtgct gatccgggcg gcaattttgt gatgatcggg atcggcttgc
    39001 tgaaaaaatc cgacgatcag gcacacaacc ggttcggata ttaccagata accgaccctg
    39061 gaaaatggaa gatcaaaaca agaggcgatg aaatctcgtt caaccatgtg ctgaaaagtg
    39121 aagtgaattc atatgattat acaaaaacag taagactcgc cggtgataaa cctattatgg
    39181 aaattgacca caagctcagg aacacaggaa aaaccagaat tgtaacaaat gtttacaatc
    39241 atcatttttt tgtaatcgac aaccagccgg tcggtcctga tttcacaata aacttccctt
    39301 ttaaactttc gggtgaaggg cagggtttcg gcagcattgc tgaactgaat gataacagca
    39361 taatcttcaa tcgtgttctg gagaaagatg aaacagtatt ctgtggttcc ctgaccggct
    39421 tcagcagcaa tccggaagac ctggatgtca ggattgagaa caagtcagcc ggggcaggag
    39481 tgaggatcac gggcaacagg ccgctgtcaa agcttatttt ctgggcctgc cgtacaacac
    39541 cgtgtcctga gacctatgtc gatatcaata ttgagcccgg aaaggagttt acctggaaat
    39601 tcatatatga attttattcc ttttagcata aaaaagccgg gaaaacatat cggatttttg
    39661 tgcaggtgtt gtttttatca tcccgtacca ggcaagaagc acccggggaa atggattttt
    39721 ccctgccaca aaagacccca tgttgacttt taaaaaagca ttatttaaaa acctgcaaca
    39781 tggggcgccc gatccaaacc tgcggctgct caagattgaa aatacaggcg atggtggagg
    39841 cacagtcaaa ctgcatcatg ctatcatcaa actgacctcc tttctttatg ttttttcctg
    39901 aaataatgaa gggggtctcc atttccaaca tagtttttcc tccgtgtccc tttccgaccc
    39961 cgccatgatc tgatgtaatg ataataatac tgttttcata aatgcctgct tctttcatag
    40021 ccgcgataat cctgccaata tagccgtcaa gttctttcag gttttcgtaa taggccggag
    40081 tatcatgccc atcgacatgg ccgacatgat cggggttgtc aaaaataatt ccggccagca
    40141 caggcttttt atccctgata tattcgacag ccatatcgca taagccggaa gggttcttgc
    40201 tgtaatcggg agcctgtgcg taataattca gggtcagggt atcagaaaga tactttatcc
    40261 catcccaatc ataaagcatg cctatttctg cgtcaggggc cgcatcacgc aataactgga
    40321 aaatattcgg aaaaattccg ttcctgttca gcacgcgtga aggaagctcc ggtaccctgg
    40381 agccccattc ggtatatccg tgaagttccg gacaggcgcc cataaacatc gatgcccagt
    40441 taactgcact ggatgagggg agtacacttc ttttgctcaa tgtaaatgaa ccattctgca
    40501 tcagttgctt aacatttggc atatcagctt tttccacgct gtatgcgccc cagccatcaa
    40561 ggccgatcag tacaacatgg ccggctttcc agccggtacg tgatttcctg cttccgggtc
    40621 cggcacagaa tgtaaaaaga actgccaggc tgagcgcaat gcattgttta attatggaat
    40681 acttcatgtt acggttattt gcttaatata tcaggaatgg gtgcaagtta acaaattatg
    40741 gcttctgcaa aattattccg ggcatgaatt caccggttga aggccacagg cactccggcc
    40801 cctggctgag tatgaaaacc ctgacaaagg ttaccggttg cacacgcagt tgcatcctgc
    40861 atcaggcaaa tgctgattgc cccggagtgc atgaatcata gtaggggcaa aaacaacagg
    40921 atcgctaccg tgagagattt tcagacatga aggtgttgtc gggaatactg gccgaggtgt
    40981 ttatctgttc aaactgaacc ctcgacgttc tcccatttat atgatttctt gcaaacatat
    41041 cggtcatgat gtaggcttcc ctgctgcccg gccttggtac agttgcattg atttcgattg
    41101 tcttgaaaag cgtaccatcg aaattgtaaa aatcgatctt tttgattttc aggtcattct
    41161 tatcgagagt agtcactttt cttgaatagc cgtattcatc tgccaccttg tcattcaccg
    41221 gtttgctttc gatgacccac agtccgttac tgcctgattc cggcatatgc ctgatattga
    41281 aatcagtatt tgatcccgaa gtcatatcgg cattcgagaa ttccgagctc ataaagcttt
    41341 tgcccttttc attgctgaca atgcgcctgg tttttttcag agccgggaga tatatccaca
    41401 tatcgtcctg ggtattctta ttgtcgataa taagcattgc ggtacccctc acatcggccg
    41461 gctccaggaa tttcatcagt cgcttttcgg tttctccgta tgtttttgag accatattca
    41521 ccttccgggt ccgtactgca ccgtttttct cagtaattgt caatatcacc gttgattgca
    41581 ggtttcccga cattgtaaga tcgcggcttt tgtccatcag ctgcacagga tcagactgct
    41641 gtgctttcaa gggcagcagg aatgctgtca gcattgctgc catgagaaaa aatgttcttg
    41701 tcatatttgt cattgttttt agaagtttta tttaaatcag cctcaataaa tgcaggcctg
    41761 aaccatagca taaaggcagg gatcacaatt atcgcacata gcaggcaaga acctattgag
    41821 atcagtacca ggtaaccgaa atatctgatt gaaagaaatc ctgatataaa ggttgctgaa
    41881 aagccagcca taacactcaa ggcattgatc acaatgctcc tgccggttgt tgtcagggaa
    41941 acggagatgg cctttgcatg actgagactc ctccggaatt cagtattata tctccagagg
    42001 tactgaatgg taaaatcaac tcccacaccg atcataattg aagaaagtaa ggcagttgcc
    42061 gcatcaatgg gtatattggc atatcccatg attccaaact ggatgatgat cgagactccc
    42121 agcggaattg agccaataag gccgcccctt actgaacgga aaattacaga aagtaaaaca
    42181 aacactgtca caagggcaaa gatcagtgag gaaatctgac cgcggatgat tttgtttgca
    42241 aagtcggcca tgattattgc atagcccccg acggtcactt cggcagggaa tccggccgta
    42301 aagctgcgta tatcattcct gagggcactg attacatgat tatcaggttt tgaaagtttg
    42361 accagcagat gggccctggt gttttcaaaa ttcatcagtt gtttccagtc gtcgggatta
    42421 ccgctcatat tatataattc aaacatctgg gcaatggcct cgtatgaatc aggtatttta
    42481 tcatacccgt tttcgccggg atcatagagt gccttgctca tttccctgac tacatcggag
    42541 atggagaaaa cgtttcccac gccatcctgt gacttaagat gagatgtaag ctcatctatc
    42601 tttttcataa ccacgggatc cttgatatct ccgcttacca ttacggatat tgtttgcgat
    42661 ccgccaaatt tcgaattgat tacccttgag gccttccgta ccggatgatt ctgggggaaa
    42721 tagttttcct gattggtatc aattctcagg aaagcaatgc cggtgctaat tacaactgta
    42781 attaccgctg ataccagcag tacacgtccg ggcttaccga caaccaagtc agaaagttgt
    42841 gcaagaccgc ggctgagaag gtctttcgaa aggtcgcgtt cttttttccg cttcagggga
    42901 atcaatgatc gtaccggatc cttccctttt gaaggtcttt ccctcagcca gatgacagaa
    42961 gggataagca gcaggctcat cagcagggcc agcgataccc cggtagcagc cagcacacca
    43021 acctgtcgtg caggaatgat ggagtgggcg agaagtccta gcagtcctgc tatggtagtg
    43081 agtccgctga aaagtatcgg catgttgagt gaaccggtta tctttctgat cagatctctt
    43141 ctcccgggag gagtttcaag gcttttcagc tcctggtgtt tggaaacgaa gtaaatgcca
    43201 tagttgtttg caactgaaat gaggataatc ggcaccagga ggctgagtat ggaaaacttc
    43261 catcccagca ggggtataag tcccatggtt gccgtaactg tcagtacaac cacggaaaac
    43321 gggagcagaa cgctcttcca ttttcccagg ctgagcttaa gcactgaaag catgattatc
    43381 agggcgatgg gaatcagtat cagggcatca tgattaacat cgctgactat gtggcgtctt
    43441 atgtagggaa gccccccggt aagaacctca gcaccgtttt cctgggcctt tgcaatggaa
    43501 tcaacccggt cgagggtcaa atgctcgggt attgccggat tgactgttcc ggtcaccgca
    43561 gcagccgtca tgtcctggga agcaacgata ccgagggcaa agcggttttc aagaatatcg
    43621 cgtctcaaag cttccatctt atcaggagtg tcaggaattt gcctgacaag aggatcgaca
    43681 accatcattc catcctcacc tgtaatcttt cgtgcagtaa aaagagagtt aacggtacct
    43741 atgccctcaa gcctcgagaa accacggtct atttccttca acctttccag gttaccttga
    43801 gtgagaatag aactgtcctc aaggatgaca atgatcatat cctgaacccc gaagtctttt
    43861 tctatggagt cagtatttat tcttgaaggc atagaatgcg gtacgaagtt cctgatctca
    43921 ggatcagtct caaccgtcgg tatcagcaga ccaagcgaca gtgcaaccgc cagcgatact
    43981 gagataatta accacctgta ttttataatg aaatcagcca taatgtcaga aatctatttt
    44041 tattgagaca aaagcggctt tcatgaagtt atccacgata tcgtagagcc ctcccttctt
    44101 ccctgaataa tgttccattc cggctccaat ggtcagtccg tctgccggct taaatctgat
    44161 catggggatc agcagcaggt cttttgaggt gaaattgtaa atggtcatca ccgatggcag
    44221 cagcctgccg taaaaaagct ccgcctcgaa ccgcagcccg gcagagtgat agtattcgta
    44281 cacctgattg ttatacagcc gtccaaatgc ctcagtctgt agccgtgtga actctaccgg
    44341 gtcaaatccg ggagtggcga acaactgcga cagttttaca agatccggtt ccgtccctat
    44401 aacaggatca tatggcgcag gataataatc gagaaccttt ctgcctgaat attcaaaggt
    44461 cattctgaag tttcccggtg tccagtcggc gcctgctacc cagtctatct gaggaaaggg
    44521 tatctcctcg tttccctctt tcttcctcac aggcttgctg tatgccaggg cgcccctgag
    44581 tacgagcggg gataccggaa cttcaaaatc aaggccggta tgattgatga tgtatggcac
    44641 tccggcaatg gcaatctgcg gatttaacag gttagagaaa tcagctgaaa ccagggacag
    44701 cccgggcata aggtccggcc cgtggtacca ttggatgcct gcatcaaagc cgcttaacag
    44761 aagatcgccc ctgaagccca gggaataata gccgttaccc ctgtacagcc cttccggcat
    44821 gctcaggtta atattagggg ggagctcaag cggagctgtc atcagaacag atgggtgcca
    44881 caggggagtg gcaactgcag ttaacttcag gaaagaagcc ggggtaatgg tcagttcccc
    44941 cgccaggttg cccatgtcct tgtcttccag atcgggactc cggtaggtat aatcgagggg
    45001 agagaaacgg gagaggggag agaaaatgtc tgtctttccc catttaatga tcctctttcc
    45061 gagcgaaatg ctaatgaagc ggttataata cattccccat gcttcccgaa ccaccaggga
    45121 atttacattg tccccaaact gctgacccat cctgagtctt atatcaccga aacccttgaa
    45181 actcatattg tcagtggcag tcagtgtaaa tgctgcatcg gcaaaggctg cattcacatc
    45241 attcggataa tctccggtac tgtaaaacat accgcccctg agaaatccac ccggagtaaa
    45301 tactttctcc tgggcagaaa gattaaaaat cagtgccgta aatatcagtg taaaaagaaa
    45361 tctttttgtc atacctgttt ttagggcaaa gatgggcagg attgcacagg caatcgtcct
    45421 gaccgcataa tccggacatt cgtaccgtgc aaaacaggtt ttaaccgagt ggctcgtaca
    45481 ttcactctga cttatgaatc agttccctgt aggcacttgg ggtttttcct gtataggttt
    45541 taaagaagtt gttgaatgtg gattttgagt taaatcctga atcaaatgca attgcgagga
    45601 tggtaaaatg ctgatatctg ggatcgctta tccgtttgat aacctccctg acgcggtatt
    45661 cattgatgaa aagaaagaag ttctttttat acttctcgtt cagaacctgt gtgatgtgat
    45721 gtcttgatat tccggttgcc tcagacagat catgtattgt aagatcacca ttgagaaaag
    45781 gtttttccgt ctccatatag ccgaggagta ttttaaggta ttcatcagcc tgctcatccc
    45841 tgagcccgct cctggaatat ttctcggggt cagctctttc agaaacctca tcccggctgt
    45901 tctgatgtac cacttccccg aaaattgaag gctgctttat tccgtaaaaa ctatatacaa
    45961 aggagaaaat ggcaatgaaa ccaaatatta caaaatacgg atcaaacgga atataattgc
    46021 cgataatgtt caggcctccg agaatgaata ggacgaaata agcaacatag aagctgactg
    46081 tcaatatttt cagccagtta agggtcattt tctgtgaggt gtatgatatc aggttcctga
    46141 gattggactg gtgttttctg atctcaacaa aagcaagaat gctataaact gtaatcgaca
    46201 gaaagaaaac caccgaatat acaatcctca ggggcattag tttatcgggt ttgaaaaatc
    46261 ccctcagatc ccttaccagg ggctctgacc tgaagaccac cgaaattgta aaaaaaacca
    46321 ggaatggaat gaaatggagc aatccggtcc agagaaattt cctttctgga accgtcatga
    46381 aacggatgta aagatacaga agcggaccat aggtaaatgt tatgaaagga aatgaataca
    46441 tctcaataac ccggctgttg accagggcaa agatcatttc aatacaaatc atgaaaagcc
    46501 aggctgccat cagcctgttt gcaaccgtaa ctggtttttt ggttgatatc agcaatcctg
    46561 caaaaaacga ctgggaaata ccaatataga gtaccggttc aatatttacc ataatgcggg
    46621 attgttttgt tccttaattt ttttcgtgat aaattcagtc caaaaagtgt aaaacattaa
    46681 aaagcctgag tttactgtta tatcctgcca taagtttttt atttttggca tgcataggat
    46741 tcgatcggaa atctttttca aagataggtg ttttggcaga catttaaacc tgttatattt
    46801 gttgataacc ggcattctgc caccgggagg ggatgcaggt gaattattct gccggccggt
    46861 ccatgtcaca aggcggcgtg aacagtcctg tgcaggttga cacataggtg ttccgggtaa
    46921 aaatacaggt attaacataa caggctaaaa aaatcctgat actttaacca gctttattca
    46981 atatgggtat tcttgtcagc aggaaatcaa gggtaattat tcagggcttc acggggagtg
    47041 aggggacgtt tcatgcacag cagatgattg attatggaac aaacattgtg ggcgggatca
    47101 cgcctggcaa gggtggaagg ctgcatctgg ggctgcccgt cttcaacacg gttcgcgagg
    47161 cggtaaggtc gaccggggct gatgtatcag tggtattcgt tcctccggcg gccggagcag
    47221 atgcaattat ggaagcggcc gagggtggaa tcaggcttgt ggttgcaatt accgaaggaa
    47281 tacccgtttc cgatatggtt atggttaaag agtatcttaa atcgctcaac tgcagactta
    47341 taggacccaa ctgccctgga attattactc ccggggaagc aaaagcagga ataatgccag
    47401 gattcatcca taaaaaggga catgtgggaa ttatctcaag atcggggaca ctgacctatg
    47461 aagctgttga ccaggtaaca aggcttggcc tgggtcagtc gacctgtatc ggtataggtg
    47521 gtgatcctgt agtaggtaca accacgctcg aagctgtaaa actaatgatg aatgacgatg
    47581 acacacatgc aataataatc atcggggaaa ttggcggatc aatggaatcg gaagcagcag
    47641 aatggatatc cgctaacgga accaaaccgg tggtgggatt cattgcaggc cagactgctc
    47701 caaaaggcag gaggatggga catgcaggtg ccataatcgg aggcagggat gacacagcag
    47761 cagcgaaaat tgaaattatg aaaaaatgcg ggatcacaat cgtcgaatca ccagccgata
    47821 taggatcatc tgttcttaat gttcttgaaa agcggtgata ccactggcac aaaggtgcaa
    47881 aaccggtctc ttcactgtac aagtacaggc gccggacccg cagatgactt tgaaacttgt
    47941 gaataaacat attgaaagac tttatttata tttgcaactg tttaaatctc atgagaaatg
    48001 aaattacttg aaggcagaac cgccctgatc acaggagcat ccaggggaat tggcaaggcc
    48061 attgcccata aatttgcccg tgaaggggca aatatcgcat taaccaatat atatgaggat
    48121 gaggagttcc tgagtaccat taatgagatt aagctttata acgtaatggt aaagggatat
    48181 gtgtcaaatg cctcgaattt tgcggactca caaaaagttg tggatgaaat agtaaaagat
    48241 ttcggtcaga ttgatatcct ggtcaataat gccggaataa caaaggatac cctgttaatg
    48301 agaatgactg aagagcagtg ggacgctgtg attgcagtca acctcaaatc cgtgtttaat
    48361 ttcaccaggg ccgtgattca gcccatgctg aagcagagaa acggctcaat aattaatatg
    48421 agctccgtcg ttggagtttc cggcaatgcc ggccaaagca actattccgc atcaaaggcc
    48481 ggaatactgg gctttactaa aagtgtggca aaagaactgg gatcgaggaa tatcaggtgc
    48541 aatgccattg cacctggatt tattctcacg gaaatgaccg acaatctccc tcccgatgta
    48601 aaagccgaat ggacaaacag gatcccgctg aagaggggag ggacaccgga tgatgttgcc
    48661 aatacagcat tgttcctggc ttctgagctt tcatcatata ttaccggaca aacaattcat
    48721 gtttgcggtg gtatgttaac ataattttat tatatttgta aaaccgcagg gcacctatcc
    48781 ttatagcacg aggaaatgca gcgagacata tttattccac aattatttta aacaggtgcc
    48841 ctgcttcttt ttattttacc cggttaatta atgaataacc ctcttttcct gctcattgtc
    48901 ctgattccgg tttccggctt tttacttgag cgatacctgg ttttcctgaa ctcaaaaatg
    48961 tggtccgaca aacttcctga agaacttaca ggtatatgtg atgaagcaga gtacagaaaa
    49021 acactccatt atgaaaagga taacaggaag ctgtcattct ggtcctcttc attcaatctt
    49081 gcgataatcc tggccatgat cattatggga ggatttgcat tcttcgacaa gctggccgga
    49141 tccatcagca cgaacatgat tgttatttcg ctcatcttct ttgccttcat cggcattggt
    49201 tcggaaataa tcaatatccc gttcaaatgg tatgacacat ttgtcattga aaagaaatat
    49261 ggtttcaata caatgaccgt caggatattt ataacagatc accttaagtc atggtttatt
    49321 accatgatta tagggatccc ggttctggct ttgatcacct ggatatatta caggctggga
    49381 aagaatttct ggctctgggc atggggcctt gttacggtac tttccgtgtt catcaatttt
    49441 ttctattctg aactgatcgt tccgcttttc aacaggcaga cacctctccc ggaaagctcc
    49501 cttcgcaccc gcatcgaaac atttgcccgg gaaaccgggt tcaggctcag agatatatac
    49561 ctgattgacg gttcaaagcg gagcacgaag gcaaatgctt atttctcggg attcggttct
    49621 aaaaaacgta tagtgcttta tgatacgctg ctgaaggaga tgtctgaaga agagattgtt
    49681 gcagtgcttg cccatgaaat tgggcattac aaaaagaaac atgtgttaag aaggcttctg
    49741 tcatcaatat tcataaccgg gttcatgctc tttctttttt ccctggtggt tgacaatccc
    49801 gccctctcgg aggccctggg tgccggaagg cccagttttc acattggact ggttgccttc
    49861 gggatcctgt acagtcccct gtcgcttgta atcggactgg caggcaattt cctgtcgaga
    49921 aggaacgagt ttgaggctga tttgtatgtc cggcaaaact ataaccctga attcctggca
    49981 agtgctttaa aaaaactctc ggttaaaaac ctttcgaaca tgataccgca tcccgcctat
    50041 gttttctttc attattcaca tcccccgttg ctggcacgac tggaaagatt gaaatgattt
    50101 tctatatttg cagtgtttta tgccccctta gctcagccgg tagagcagct cattcgtaat
    50161 gagcaggtcc ggggttcgag tccccgaggt ggctcagggc aatttcagaa attgaattgc
    50221 aattgacaag ttacggataa ttgctatttt tgaacctgga atttccggga ccttataccg
    50281 gagatttcgc gtgggaagtc cgaaatgtca gatgcgggaa tagctcagtt ggcagtcccg
    50341 ggcaatcggg atcccaggcc gagggcctga aaaggaaatg cgggaatagc tcagttggca
    50401 gagcatcagc ttcccaagct gagggtcgcg ggttcgagcc ccgtttcccg ctcaaatcaa
    50461 accggggaat cagtttaagc tgattccctt tgctttcctt aaggagaagc attaattccc
    50521 gggctgatga agccagtatt gttgatgcag caacttctgc ctgaaatcct ctgactcctt
    50581 aaagtatata atccgtaatt attaatattg cataaaccat tggccctgaa caatcgcgga
    50641 ccgaaatttg ttcttcagaa agccggtcct gccggattcc ggacttaccg gttcgccggc
    50701 atatgtggaa ctgaataaaa tcatatggca gaacgtttgg gatactcaaa attgtcagta
    50761 ctaaaaaaca gatatgtatg agctacactc ttaagcctgg ccagaaggcc atcgacttcc
    50821 ggctccctgc aaccgatggc agggaataca gcctttcaga cttcgacaaa tacccgtacc
    50881 tggttctctt tttcacctgt aaccactgtc cttatgtgac cggctccgat gaagtgacaa
    50941 gggcaactgc ggaaaaattc cgcgacaggg gagtggtatt tgccggcatc aattccaaca
    51001 gtgagaatac ctacgaggaa gacagcttcc cgaacatggt caggcgcatg aaacagcata
    51061 acttcccatg gaaatacctt catgacaggg agcagaccgt ggtaaaggct tatggcggcc
    51121 tgagaacccc gcattttttc gtattcaacg ccgaaaggat acttgtttac acgggcaggg
    51181 gcgtcgattc gccccgcgat atatcccgca tgacagttaa tgacctcgac agggcactcg
    51241 aagaacttac ttcaggcagg gaaatctcag taccggttac taaccccata ggatgcaaca
    51301 tcaaatggga aggcatgaat gccaaatgga tgcctccgga ggcctgtgac cttgtctgat
    51361 atcccctgac atacgggtac ttattcaatc agtcatttgc acagcaggca aacaggctgc
    51421 ttaatccggg taattcgctg tgaaattgca aaaatcctca gaatgaacct tataaatatt
    51481 caatattgca aaaccaggat aggtgagctg atactgggtt catatgccgg caggctctgc
    51541 ctgatggatt tccgttacag aaaaatgaga ccggctgttg ataacaggat aaaaaaaggt
    51601 cttgcagctg aattcaggga acagaatgat gagattatag cggaagcaat ccggcagctc
    51661 gaagagtatc tcgccggcca cagaacagca ttcgatatcc ccgttctgat gattggaaca
    51721 gattttcaga aagatgtctg gagggcactg acggaaatac catatgggaa aaccgtcaca
    51781 tatacggaac ttgcaaaggc aataaaacag gaaaatgctg tcagggctgt tgcgggtgca
    51841 aatggagcaa atgccattgc gattatcatt ccctgtcatc gtgtcattgg aagcagcggc
    51901 gagcttaccg gttatggagg cggcctgtca gtcaagaaac ggctgctgaa actggaacgt
    51961 aataacagtg tcggtcaact gcctaaaagt attttttgaa cttttccccg atcttgaagt
    52021 aggtgtacag tcccgcatct tctccagccg cacggacaga atcaccgtcc acctcgtatg
    52081 tgctgtgctc acctgccacc tgtgaaaaat aaacggcaaa tcctttgcct tcctggtcct
    52141 tcatcagtac aagtgcaaga tttttatgga tatcggattt cagtgaggcg ctgcagagag
    52201 gacaataaaa gtcaaccagc tctccctgtc cgaactcaaa ggaaggatgc tttatgcttg
    52261 aataattgcc aatctgcgga ctgagcagga taagcccggt ctctttcctg tcatttttta
    52321 tcttaaagat tatatgatct ccgacccgga gatgcccttt gcacttggga cacatgaaat
    52381 cattcatttt cacctcctgt tttaaattga caatgaataa tattataaaa ttaagaaaat
    52441 gaaaggatat gatcgctgca actgagaaaa accggaaaaa taatttcaga tgccggggat
    52501 gcctgtgcct gaatgctgat tgcgcgccgg cttttttaac tcaactgaac ccgggctgaa
    52561 agaataacct cgcattgaag aaacagatct tattatgaag tttttccacc atcaatattc
    52621 tgccgttaaa ggccgacaac caagcaggct cgtccgacac aacgatattt attccctggt
    52681 aatggtcatg cttattttaa aatctttctg atctgtggtt ctattactta cagggtaggg
    52741 taatacctca tttatcctga atgaatagcc gccgatgctg acaggatttc tggctgcatt
    52801 attaagattc atcatggtat caatgctggc ggaggccagg taaaacttca taaatacatc
    52861 aacatccccg gcccagacgc aaacaacatc cgacgggcac cggtaatccc taatactgtc
    52921 gattgcaaag gcaaggttcg cgtcaacccc gaaacggtga ttgacaatac tgtcaactga
    52981 ccggccggat tctgtttttt cacatccccc tgaaaagagg aagatcagaa aaacaccgga
    53041 aaagaatatc ctgaatttca tattacgtca atcaatatgc attttgcaga cttttcagaa
    53101 tcaaaaatcg taccgcaaat tgccggaagc ctgaaaaatg attaaccttt tcacggtgtt
    53161 atagtcttaa tctttgtccc gggcatcccg aaggctggca gggaattctt ttcccgggat
    53221 cattgttatt tttttccatt acctttgcct cctgttttac acaattttcg gaatgaaagc
    53281 tgaaataatc acaataggcg atgagctcct catcggacag acaatcgaca ccaattcggc
    53341 gtggataggg tgggagctga gttctgccgg tttcgatgta cggagaagga catcaataca
    53401 tgacaacagg gatgagatcc tcaatgccct ttcggaagcc gctgaaagga aacctgaact
    53461 tgtaataata actggcgggc tcgggccaac gagcgacgat attacgaaac aaaccctctg
    53521 tgaattcttt aacactaagc ttgtacctga cagggaagtg ctggaaatga tagaaaaaat
    53581 gctgcggagg aggaattttc cgatgaatga gaataaccgc agacaggcag aggttcccga
    53641 gtcatgcagg gtcctgccaa atgccgcagg caccgccccg ggtatgtggt ttgaaaagga
    53701 tggcaccata ttcatttcaa tgcccggtgt gccgggtgaa atgaaatata taatgcagga
    53761 gcatgttctg cccgatataa agaaacgatt cagatcgcag gttataattc acaaaaacat
    53821 aatgacttac gggatgcctg aagcacggct ggcagagatt cttgaacaat ttgaagcgga
    53881 acttcccgaa aacataaaac ttgcttacct tccatcattc ggggtgatca aactaaggct
    53941 taccggcact gcaggaacac tcaacgaaat tgagccgtta atcggggccc agaccagtaa
    54001 gctctacaat atcattcctg accttatttt cggtgaggac gaacagacac ccgagatggc
    54061 tgttggtcaa ttattgaaaa taaaaaaact gacactttcc ctggcagaaa gctgcactgg
    54121 cggtttcctt gcgcacagga tcaccagtgt ggcaggaagt tctgagtatt ttgccggatc
    54181 tcttgtagca tatagtaaca atgtcaaaac cggcatcctg ggcattgatc ctgcaatcct
    54241 ggcttccgac ggtgcagtaa gcagcagggt ggctgcaggt atggctgaag gagccaggaa
    54301 actttttaaa accgatcttg gtttagcaac caccggcatt gcaggtccct ccggaggaag
    54361 tgctgataag ccagttggaa tggtatggat tgccgtatcg tcagattcgg gaacgataac
    54421 tgaaaaacat atcttcagta ccgacaggat tgccaatatt acacgattct ccctggctgc
    54481 gatgaacctg ctgagattac agataatcaa cagataagac atatataacc cgatacatta
    54541 aaaaaattga aacatttatt tgtttaatca gataaaaatc acgtactttg cgcttcgtta
    54601 aaaaaataaa ggatcaatcg gaataaggaa tcatgtcaaa gatttgtcag gtaaccggaa
    54661 aaaaggcgat ggtgggaaac catgtttccc attcaaaaag aagaacgaaa agaaaattcc
    54721 gtccgaatct ttccgtcaaa aaattctatc ttcccgagga agacagatgg atatccctga
    54781 aggtttcagc tgcaggaata cgccttatca ataaaaaagg actcaacgcc gtactgaagg
    54841 aagccaggga gaagggttat attcttaatc actaattttt gagaaaatgg caaagaaggc
    54901 aaaaggcaac aggctgcagg taattttgga atgcaccgag cataaggaaa gcggtatgcc
    54961 tggcacatca agatatatta cgacaaaaaa caggaaaaac acccccgaca ggcttgaaag
    55021 gaagaaatac aacccgatcc tgaaaaaata caccatacac agagaaataa aataaattag
    55081 cagcgcaatg gcaaagaaag tagttgcaac actcaaaata ggcagtggta aactttttac
    55141 caaatgcatc aggatgtcca gatccgataa aacaggtgca tatcagttca cggaagaaat
    55201 cgtccacaat gaccatgtaa aggaattctt taataagaaa taactccttg catattaaat
    55261 tattacatga gctttcttcc attgaagaaa gcttttttta cttttttaat cctgcaaatt
    55321 tcattcaaga tgggctggtt cggtttcgtc agtaaggata aaaaggagct acttgacaag
    55381 ggcctcgaga aaaccaagga aagtgttttt acaaaactgt cgcgggcagt aatgggaaaa
    55441 tccaggatcg atgacgaagt ccttgacaat ctggaagaag tcctggtttc ctcagacgtt
    55501 ggagtggata ccactctgcg tatcatcgaa cggttggaaa acagggtttc aagagacaaa
    55561 tatctgaaca ctgatgaact caaccatatc cttaaggaag aaattgtatc tcttcttgag
    55621 gcgaatattt cgggaactga atacgatttt gcatcggaac tgcctttcaa accctttgtt
    55681 attcttgtgg taggcgtcaa cggatcggga aaaacaacca ctattgcaaa actggcatat
    55741 cagttcagga atgccgggaa gaaggttctt cttggtgctg ccgatacgtt cagggctgca
    55801 gccatcgatc agctgcagat ctgggccgac agggcagggg tccaggtggt aaagcagcaa
    55861 atgggttcag atcctgcttc cgttgcttat gactcggtac ggtcagcggt tgccggcaaa
    55921 tatgacgtgg ttatcatcga cactgccggc cggcttcaca ataaggtaaa cctgatggcg
    55981 gagctctcaa aaataaagag ggtaaccgaa aaaatacttc ctgaatcccc gcatgaggta
    56041 ctcctggttc ttgacggatc gaccggtcag aatgctttcg tgcaggccag ggaattcaca
    56101 gctgcgacag atgtaacggc aatggcaatt acaaagcttg acggaaccgc aaagggaggg
    56161 gttgtcatag gtatatccga tcagttcaga ataccggtaa aatatatcgg ccttggcgaa
    56221 aagcttgacg atctgcagat cttcaacccc tatgaattcg ttgattccct cttttcagcc
    56281 gccaggtaat caccgaaaac ggttccggtc gcggaatcac tctgccaatg ataaatagaa
    56341 caataaatat agtaaccctt ggctgctcaa agaatctggt tgattcggaa aaactgacgc
    56401 ggcagatcga ggctgccgga tacagggtta tatacgatgg agcggaactt tcctcaaata
    56461 tcgtcattgt aaatacgtgc ggcttcataa atgacgccaa ggaagaaagc attgacacca
    56521 tactccgctt cgtagaggcg ctgaaaaagg gcgacattga aaaactctat gtcacgggat
    56581 gtctttcaga aagatatgcc gcagaattgc ggtcggagat ccctgaggtc agcaggtatt
    56641 tcggggtaaa cagcatggac cagattctca gggaactggg gggacatacc gacaaaaggc
    56701 ttctgctgga acgcaggatc agtgatccgg gtcattatgc atatctcaag gtatcagagg
    56761 gttgcagcag aacctgcgcc ttttgcgcca taccctctat aaggggaaaa tatttatcca
    56821 ggcctgttga ggaagtggtg gaagaagccc gaatccttgc ccgcaacgaa gtaaaggaac
    56881 tgatacttat tgcccaggat ctgagctatt atggcctcga cctgtatcgc acccaaatgc
    56941 tgcctgtcct gctcagggaa ctccttggaa tcgaatcttt cagatggata agactgcatt
    57001 atctttatcc gtccaatttc cccaccggta tcattcccat gatgcaggaa aacagaagga
    57061 tatgccagta tattgatatt ccggtccagc acatatccga caggatcctc aaaatgatga
    57121 aaagatcgca taacggaagc gaaacaaggg aattgctggc aaccctcaga aaagagctgc
    57181 ccggagcagc aatcagaact accctgattg ccggccatcc cggagaaacc caaaaggaat
    57241 tcagcctttt gaaggatttt gtcggtgaat tcaggtttga ccgcctggga gtatttccct
    57301 attctcatga agaaggaaca tattcctatt ccaactacag ggattccatt cccgaaaaga
    57361 taaaaaaaga acgtgtttcc gaactcatgg aactgcaaca atcaatatcg tcagatctca
    57421 atactttaaa gatcaataaa atactcaaag tgatcattga caggagggaa ggagacatgt
    57481 atattggaag aacagagcat gacagccccg atgtcgacca ggaagtattg ataaatgccg
    57541 ttcatgatct gaagcctggc aatttctata acatcctgat cacccggtca actgagtttg
    57601 atctattcgg caccccggta taaaacgggt aagcatcaat tgtaattcct attcagccgg
    57661 gggatcagga attccttcag gcgattcctg attgttctta tggatcttga tccttatctc
    57721 tgatttctgg ctgttgaacc ctatatgtat ggtatcaccg ggtttcagat cggccttgat
    57781 cagcacctcg gccatcggat cttccaggta tttctggatg gcacgcttaa gcggacgtgc
    57841 accataattt gcatcgaatc ccttttcggc aataaaatct ttcgccgccg gaacgatctt
    57901 catcttgtag cccagggcat ttaccctgtc gaacaggccc ttgagctcaa ggtcgattat
    57961 tttgtcaatt tccttttttc caagtgaatt gaacattatg acatcgtcaa tcctgttcag
    58021 gaactcgggt gcaaatgttt tctgaagtgc tttctgcaga atgcttttgg cctgctcgtc
    58081 gcggctggcc tttcttgcac tggtgtcaaa cccgataccc ctgccgaatt cagtaagctg
    58141 cctggttccg atatttgatg taagaataac aattgtgttt ctgaaatcca ccctgcgccc
    58201 cagactgtca gtaagctgtc cttcgtcgag tacctggagc atgatatgga acacgtcggg
    58261 atgagctttc tctatttcat caagcaggac cacagagtaa ggctttctcc tcactttttc
    58321 tgtaagctgc ccgccttcct cataaccaac gtacccggga ggcgccccga caagtctgga
    58381 tacagagaat ttctccatgt actcgctcat gtcgatccgg ataagattgt cggtggtatc
    58441 aaagaggaat ttcgccagga ctttcgcaag ctgtgtcttt cccacgccgg tagggcccag
    58501 gaaaataaat gtaccaatgg gcttgttggg atctttaagg ccggccctgt tgcgctggat
    58561 tgacttcacg atcttctgga ttgcctcatc ctgtccgatc acacttccct tgagctcctc
    58621 ggccatttta agcagtcttg ttccttcagt ctgggcaatc ctctggaccg gcacacccgt
    58681 catcatggca acaacttcag caactttttc agcatcaaca gattcgcgat taacggcaag
    58741 ttccttttcc cacttcttct tttcatgctc aatcatatcg ataagatcct tctccttatc
    58801 acggaaactg gcagcttttt caaaattctg atttttcacc gccatcattt tttccttctt
    58861 ggtatcatcc agctgctttt ccaggttcaa tatccgctcc ggcacgacaa tatttgatat
    58921 gtgcaccctt gatcctgatt catcgagagc atctattgcc ttgtcgggca gatgccggtc
    58981 agatatgtac ctgtttgtca gctttacgca ggcatcgatg gcatcgtcgg tataaatcac
    59041 attatgatgc tcctcgtacc tttcctttat gttgtgaagg atgtttatcg tctcttcaat
    59101 ggaagtgggc tccacaatga tcttctggaa ccttctttcg agtgctccgt ccttttcaat
    59161 atgctgcctg tattcgtcaa gcgtggttgc cccgatgcac tggatctcac ccctggccag
    59221 ggcaggcttg agcatatttg cagcatcgag tgatcctgtg gctccgccgg ctccgacaat
    59281 ggtatggatc tcatcgatga acaggattat gttgtcattt tttgaaagct cattaagaat
    59341 ggccttcatc ctttcctcaa actgtcccct gtatttggtt cccgcaacaa tggaggcgag
    59401 gtcgagggca acaaccctct tgttgaacaa taccctcgaa acgttcttct tggttatcct
    59461 c
//
LOCUS       DAOOYG010000002        58404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_435_length_58404_cov_7.392757, whole genome shotgun sequence.
ACCESSION   DAOOYG010000002 DAOOYG010000000
VERSION     DAOOYG010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 58404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58404
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_435_length_58404_cov_7.392757"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1057
                     /locus_tag="PK288_00285"
     CDS             110..1057
                     /locus_tag="PK288_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPM17383.1"
                     /translation="MNRVPSLDDIRSAHLRILPYINRTPVLTSGQLNRIYGCSLFFKC
                     ENFQKTGSFKFRGAVNAVLQLNEEQKKGGVVTHSSGNHAAALSLAARMNGIGAYIVMP
                     ETSPDVKKKAVAGYGAGITFCKPGQAQREAACRELMNEKKATLIHPYDNFNVICGQGT
                     ASLELLEEVKDLDVVVAPVGGGGLLGGTSCCVKGTDGRIRVLGAEPVNANDAWKSFKT
                     GKLTPSVNPLTIADGLLTSLSELTFNIIRNHSDDIITVSEKSIIESMKLIWERMKIIA
                     EPSSATVLAAVGENPGQFRGKRIGLIISGGNVDLTRLPF"
     gene            complement(1054..2139)
                     /locus_tag="PK288_00290"
     CDS             complement(1054..2139)
                     /locus_tag="PK288_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPM17384.1"
                     /translation="MKKLIYKIVLTVNILFALALLASYISVHIRPDIFAFPALLGLSY
                     PYLLLINILFALLWIIFLKAEAFISIAVIAMGLTHFSNYIRLRKPSGDKTGTFEVLSY
                     NVRLFNYYENKKPVRSERRIMELLKNHQPEIICLQELYFQGDPERKEAALRSALGNRY
                     HSHLKVISTGRNRYYGVATLTRFPIVNRGEIYHPRSSSISIFTDIVIESDTFRIFNNH
                     LESFRLQRRERSFAAELTEASGDKEAVSELRSISRSLRQGYKRRAAQAQILKDRINKT
                     SYPVIVAGDFNDTPVSFTYRKIRKGLNDSFLTSGYGAGFTYRGNYPPNRIDYILYGNG
                     LECRQFDIMKINYSDHYPIIAYFRKKN"
     gene            complement(2253..3143)
                     /locus_tag="PK288_00295"
     CDS             complement(2253..3143)
                     /locus_tag="PK288_00295"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPM17385.1"
                     /translation="MHLFETIRASFRKGTSLTRLIYINIAVFILIAAGSIVSFLLDNP
                     ELQAKIVNILAVPSSFTSLLKRPWTLITYMFTHKDVLHILFNLLWLYWFGLIFLEYLD
                     QKKMVAVYLLGGLSGALVYILSFNIFPVFGKVVDTSVAIGASASVMAIVISIAAWVPD
                     YSVSLLLLGRIRIKYLALAIFVLTSVLDFSVNSGGKLAHMGGALFGYLYAVNLHRGRD
                     LGKWINRVIDFFVTTLRPREKKIRVTNKKPVSDYDYNKRKTERQEKINIILDKISKGG
                     YDSLTKEEKDFLFRESQNKN"
     gene            complement(3143..3886)
                     /locus_tag="PK288_00300"
     CDS             complement(3143..3886)
                     /locus_tag="PK288_00300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPM17386.1"
                     /translation="MNGFGRSFFALPPVVKNLILLNVLMLLANWASKSVFSTDLTMIL
                     GLYFPKSEHFMPIQIITHMFMHGNFLHLFFNMFALFMFGGILENVWGPKRFFIYYIIC
                     GLGAALVHETVIMFQYNKVMGIISPDQLQLVLNDGASYLSQGKVFTNETMKTLQMLLN
                     VPTVGASGAVFGVLLAFGVLFPNTQLMLLFPPIPIRAKYFVLGYGAIELYLAVTQPGS
                     NIAHAAHLGGMLFGYFLIRYWRKTTRTLY"
     gene            complement(3996..5789)
                     /gene="mutL"
                     /locus_tag="PK288_00305"
     CDS             complement(3996..5789)
                     /gene="mutL"
                     /locus_tag="PK288_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPM17387.1"
                     /translation="MADIIRLLPDSVANQIAAGEVIQRPASVVKELMENSVDAGADNI
                     ELIIRDSGKTLIQVIDNGTGMSETDARLSFERHATSKISSANDLFAIKTKGFRGEALA
                     SIAAVSMVELRTRKADTDEGTLIIINGSKVEAQEPCSCMPGSCFSIKNLFFNIPARRK
                     FLKSDNTEMRHIVIEFQKIALAHPEIRFQLLHNNDVIYSLPAGNRRQRIVGVFGRQMG
                     NDLIPLDTSTNLVNVSGYVGKPENARRTYGEQFFFVNNRFMKHPYFHRAVTEACQNIL
                     PAETIPSYFIFMEVDAASIDINIHPTKTEIKFEDERTIWQILMASVKEALGRFNIVPS
                     IDFSSEMLIDIPVSTIPGNTPVPPEIAINRQYDPFAGENSVSHKQQFIHNFEKEHHAH
                     WEKLFPPADLPGRKSPHTEGERRFFQLGGKYIACPVKSGLMIIDQKRAHERILFEKIL
                     KNKGPANLSQALMFPVSINLNPADYFLIREIEEDLQKTGFRFRLADPDMIIITGLPAG
                     LNISDPAPMLEQFLEEYKNSPADPSEGTRNKVAAALAAASSIPHGRILFKDEMEDMFD
                     TLFACSSPNFTPLGKPVLSIIDLAEIEQRLK"
     gene            5941..6819
                     /locus_tag="PK288_00310"
     CDS             5941..6819
                     /locus_tag="PK288_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPM17388.1"
                     /translation="MKKFVLLASLLLILLCGCKRKPGENDLKVITLNVRYDNPGDSMN
                     AWPNRIPLVLEFFRDQKPDLIGLQEVLFHQYELLDSVWKDYSSVGAGRDDGAKSGEMN
                     PVFFRKDKFDLIRSKTFWLSEAPEIPGSMAWGAGLPRIVTWVELADKSTLEHFYFFNT
                     HFAHDSDSARIMSAKLLLSKVDSLAAGFPFIITGDFNMLHSSQGYSILTGPYESVPLL
                     ADSYAITDKRPVGPAYTFHGFSEKSSTGRIDFIFVRNGMKVLEHRVFIRKDHGIYISD
                     HWPVMAVVELKPSRHK"
     gene            6979..7821
                     /locus_tag="PK288_00315"
     CDS             6979..7821
                     /locus_tag="PK288_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPM17389.1"
                     /translation="MKTEGRRTSSNIEDRRGMSTGKMAVGGGIGAIAIAVIVLLLGGD
                     PSTVLETMQSPAVESGSVTSSPEEDAMANFVSVVLAGTEDVWTRVFRESNLTYRQPKL
                     VLFRNQVQSACGYASGASGPFYCPGDEKVYIDLAFFDELRTRFGAYGDFAVAYVVAHE
                     IGHHVQNLLGIINEVNGKRARLDQVSSNKLLVRLELQADFLAGMWAHYDQKMFNSIEA
                     GDIEEAMNAAASVGDDVLQKRYQGRVIPDAFTHGSAAQRKEWFRRGYVTGDFDKGDTF
                     NADI"
     gene            7974..8699
                     /locus_tag="PK288_00320"
     CDS             7974..8699
                     /locus_tag="PK288_00320"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPM17390.1"
                     /translation="MFDRLFIISLLAGMSLLSCRTAASEDSQGSVLVRKCTDFTVTGD
                     GKSENWSSTNWIMLGRQDQGKVDYRTRAKVLYSETGIYFLFDCEDRKLTSTLKADNLD
                     LYTEDVVEVFLWTGENFPVYFEYEISPLDFELPIIIPNNKGTFLGWLPWKYEGDRRVR
                     HATSVKGGMKESGAAVKGWMAEFFIPYRLLTPLGNVPPVSGTKWRANMYRIDYDQGPT
                     HFSWQKTSGSFHEYQKFGTFIFE"
     gene            9059..10378
                     /locus_tag="PK288_00325"
     CDS             9059..10378
                     /locus_tag="PK288_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM17391.1"
                     /translation="MKNLFLSIFIAVLLPGNPMNAQEDNVWSLEECISYALEKNIQVR
                     KSELANQRNQYYADQAKAQRFPSLNASLNHNFSWSKSDGEGQTGYSGINGSNVSVNSG
                     VTLFNASRITNQIKQADLDLQAGQYSLETVRESVSLSILEAYLQVLYAEEQVKNSIRQ
                     IESTSGQVDLAAERLAVQLISQADYAQVKAQLASEKLTLANSENQLAIAKVNLEQLME
                     LPVSESFAIVHPEFGEQVNQHRLPDVMLIYETALGIKPQVKAAEVSRQIASLDEKIAR
                     AGYFPTLSASAGIGTSYSSRLTDPYFSQLDKGLSPSLGFSLTIPVYQKKQVRTGVAVA
                     KAGYRDAELNEIDTRNGLRKNIEQACVDVTSAQVKYEASLEKYNATEVSAALSDEKFR
                     QGLINSVDYLVSKTSLIVAESELLQARYNLIFSYKVLDFYQGVPLVL"
     gene            10409..11680
                     /locus_tag="PK288_00330"
     CDS             10409..11680
                     /locus_tag="PK288_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM17392.1"
                     /translation="MMKKKIVIAALITAVLTIGAVVLPPVIKKKPVYTFDTATVSRGT
                     ITNTVTATGTIEATVTVEVGTQVSGIIEHIYVDFNDNVKQGQVLARLDETALRAQLEQ
                     SQANVDQARAQLNFQEATYNRLKVLFEKNLIAQADYDQALFNYENSKASLSNARSALA
                     RAEVNLAYATIYSPIDGVVLNRAIEEGQTVAASFNTPEMFTIVNDLTQMEVQTNVDEA
                     DIGNVRQGQRVEFTVDAYPDLVFTGSVSQVRLQPVTTNNVVTYVVILNAPNPEKKLMP
                     GMTASATIFIEEKHNTLVVSGKSLRFTPDAGYLSEIMAKASGKVPNKDFPAVPVPAGM
                     AGPAGGAPPAGFAMPGSSRDPKSGIATLWVKSDKNVISPVPVTIGIENGSNVEILDGL
                     AEGAEVVIAMSGGGKKEQENRNRGPRGPFPF"
     gene            11715..12476
                     /locus_tag="PK288_00335"
     CDS             11715..12476
                     /locus_tag="PK288_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM17393.1"
                     /translation="MDNTIIRITNLRKDYFVGEITVHALRGIDMEIKEGEFVAIMGTS
                     GSGKSTMLNILGCLDTPSGGSYHLDSVDVSTMNRDELARLRNLKLGFVFQAYNLLPRT
                     TALENVELPLFYNNRIKAKERRELAMNALESVGLADRLDHMPNQLSGGQQQRVAIARS
                     LVNNPVLILADEPTGNLDTRTSFEIMELFQDLNSKGRTIVFVTHESDIARFATRNIVF
                     RDGRIQREACLTERLLASEILKSLPVEDIELTQPK"
     gene            12487..13707
                     /locus_tag="PK288_00340"
     CDS             12487..13707
                     /locus_tag="PK288_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM17394.1"
                     /translation="MDLSNLFKIAVRALVRNKMRAVLTMLGIIIGVGSVIAMLAIGQG
                     SKQKIRDEMSSMGTNMIMIMPNYMRRGGVSLGASSSMALKYSDVAAIRNEAAAVAAVS
                     PEVRSNNQVIFGNQNTQTTIYGVSEEYLAIRKLEISSGRIFTQAETRSMAKVCLLGRT
                     VIENLFGKDSDPVGMTIRIKNLPFEVIGVLKGKGENGMGMDQDDLIIAPYTTVQRRLA
                     AIDYINGIYASATSEAKSAVAIAEVEEILRRNHKLRESEEDDFRIMSQSELIETFSSI
                     SDVMTYLLGAIAGISLLVGGIGIMNIMFVSVTERTREIGLRMSIGGRGRDILRQFLVE
                     SMLLSIMGGTLGVIFGYIVAKVAGSVMNNPAIVTSHSVILAFAVCFAIGVFFGWYPAK
                     KAAGLNPIEALRYE"
     gene            13717..14823
                     /locus_tag="PK288_00345"
     CDS             13717..14823
                     /locus_tag="PK288_00345"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPM17395.1"
                     /translation="MKKKIEGNKITILLHFLAWVVIIGIPLYYIRRWPFGKDFIWINY
                     LNTLISGMIFYVNYLILVPGLFFRTKRYRYYLSVLALIVLFYFVSDIANKKAFSFVTE
                     KARSGQVDIKPEERGRMMPPPPGGLRLRFPSRHIYLFNYGFNSVFLIFFSLGLRVFER
                     NSKIEKLQKETEQEKLRSELAFLKSQISPHFFFNTLNNIYSLISINAEDSKKAVLKLS
                     KLMRYLLYESEQGTPRLSTEIDFMQNYIDLMKLRMSDKVDLSVDFPEVFDDVSIPPLL
                     FVPYIENAFKHGISYREKSFIAIRLEQEGQLLKFTCKNSIIAPAARGENREGPGIGLE
                     NAAKRLNLLFPSRHELKISKTDKVFEVFVQIDIQ"
     gene            14820..15539
                     /locus_tag="PK288_00350"
     CDS             14820..15539
                     /locus_tag="PK288_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM17396.1"
                     /translation="MRISAIAVDDEPLALQLVIGYIEKTPWLELAGSFDNPVDAMDFV
                     EEHNVDLVFVDIQMPDLNGIEFTRSVKRSPKVIFTTAYDKYAIEGFKLDIVDYLLKPF
                     SYEEFLKAVNKARRLIMLEKGTADKIESSNEFLFLKSEYKIRRIDFNDILYIEGLKDY
                     VKVYLMDQAKPVMSLTTLKLLESKLPASKFMRVHRSFIVNLEKVDTIERGRIIFGKTY
                     IPVGEQYKEKFQGFLDRNFLV"
     gene            complement(15639..16157)
                     /locus_tag="PK288_00355"
     CDS             complement(15639..16157)
                     /locus_tag="PK288_00355"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPM17397.1"
                     /translation="MEKLIITPKTKIYDLLHSYPELEEELIDAAPEFKKLRNPVLRKT
                     IARITNISQAATIGGLKVEDLVNRLRARVGQGNIEQMDEGGAKYITDRPGWFTGDMIV
                     RSIDIREMLNRDEQPVHEVLASIKKLGGNEILEVIAPFIPAPLIDKALSLEYRHWLDK
                     KSEGEYRVYFTR"
     gene            complement(16170..17405)
                     /locus_tag="PK288_00360"
     CDS             complement(16170..17405)
                     /locus_tag="PK288_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HPM17398.1"
                     /translation="MSELINNSKFRKEKLKELILKLHKGEDPQRVREELIATLRQVPY
                     GEVVEVEQELISEGLPETEILKLCDIHGEVLDGHIDQTGAAFIPEGHPVDVFRQENRE
                     LLKVIEKAQMLLGTLYSVPESGFREYSLQILSCFNELMDVDKHYKRKEYLVFPYLERQ
                     EITGPPKVMWGKHDEIREQLKGCIEILRSEDLRKSDLEDATEILFAPVLKAMADMTKK
                     EEDILFPMSMDVLTTDDWWQIHRQTLEFGYTLYDPKADWKPEGVTAENTGEDISYESG
                     TIRLPSGSFTAAEIMAILNTIPFDMTFVDRNDKVKYFTQGKERIFARNRSIINRDVRL
                     CHPPGSVHIIEKIIDDFKTGKASHAPFWIQMRGKFIHIEYFALRDENGEYLGTLEVSQ
                     DLSNARKLEGEKRILSYSE"
     gene            17615..18832
                     /locus_tag="PK288_00365"
     CDS             17615..18832
                     /locus_tag="PK288_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPM17399.1"
                     /translation="MQAASVTHPENLNNLINFAKRLIDGENGRELILMYKHSLESVTA
                     EETMKVLDHLLTCGYSFSTVKNNVGRILNAFFRSLNDHKWKRPAEPHFLYYLMLENRA
                     VEKLIAGIRPSLRLILGRDGTGSSDSLVKVRNFIMSLKEYELHYIKKENILFPYLEKT
                     FTQYRCLQLMWSFHDDFRQGLKTLEEILSEEAVDRERFNREIGKLFFVIFPIIFREEQ
                     IVFPVAMDSLPTGAWNEMLAQSFETGWCYGISPAAKDQTAVMSRNVDRVDLGTGLLKP
                     GQIILMLENLPVDITFVDENDEVCYFSGSKHRIFPRSKAIIGRKVQNCHPHESVHVVN
                     EILEAFRNGSRDQADFWIQSKGRFIHIRYFAMRDAGGSYKGTIEVSQDVTGIRSLQGE
                     KRLLDWGCLTKDK"
     gene            19010..20548
                     /locus_tag="PK288_00370"
     CDS             19010..20548
                     /locus_tag="PK288_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPM17400.1"
                     /translation="MLVRTFASAVTGIDAGTVTIEVNVSVGIRFFLVGLPDVAVKESQ
                     QRIESALRSLGYHWPGRQVVINMAPADVRKEGSSYDLPLAVGILAADEKISRGRLESY
                     VMMGELSLDGRLQPVRGILPIALRAREEGFRGIVVPVRNAREAAVVNGLEVFGMETLG
                     DVVDFFNGKRDFSPVSVNLTDLFNREANKYDVDFSDVRGQENVKRALEVAAAGSHNIL
                     MIGPPGSGKTMLAKRISTIIPPLTLEEALETTKIHSVAGKIDDHTALMIRRPFRSPHH
                     TISDVALVGGGTFPQPGEISLSHNGVLFLDELPEFKRTVLEVMRQPLEDRVITISRAK
                     STVDYPASFMLVASMNPCPCGFHNHPEKDCMCSTGMVQKYLNRISGPLLDRIDIHIEV
                     TPVKFEKLSDQGRIEKSETVRERVMKARMIQARRFNSVNGVYTNAQMSPAQQRKYCQL
                     DEAGGKLLRTAMELRGLSARAYDRILKVARTIADLDESENIAVSHISEAINYRNLDRE
                     GWAG"
     gene            20723..21838
                     /locus_tag="PK288_00375"
     CDS             20723..21838
                     /locus_tag="PK288_00375"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HPM17401.1"
                     /translation="MDFINSIHSRMVNERLLFAYRGDISEENSLPLITLIENEMKTES
                     HRLAVRKKLFMFVLESIQNIIRHGNRDHADMSMVTYARTDDGYSITTGNMIETDNVKE
                     LERRLVQINRSDEREIKSQYMQILNSSELSSKGGAGLGLLEMAIKTGNRLDYDFVPYD
                     DKYTYFVLNKTVNASGKGINRGGRMNGERKGLLDGVEQLMADNRIYMIWSGHFSSDVG
                     GEVLSITDARLTKNDLELRIRKRIFTIQMEILDNLAKYSTGRDSEKKYGLPVIMLGFD
                     GLKFILISGNLIVNSAIPELKAKLDTVNRHSIDELREMFTRSLSVQTTDNDSTGNMGL
                     IDIARKSGSKLIYSFEKINETYSYFFLTVKTGTDNDQ"
     gene            complement(21832..22581)
                     /locus_tag="PK288_00380"
     CDS             complement(21832..22581)
                     /locus_tag="PK288_00380"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPM17402.1"
                     /translation="MIKKRSKIFFASDFHLGLKAGKDPHEREKKVVEWLLKIAPEARE
                     IYLLGDVFDFWWEYKLVVPRGFTRFLGTISALTDSGVVIHFFTGNHDMWIRDYLSTEC
                     GVICHTGPFSTIFGNRRFYLAHGEGLGSNDLGYKILLWIFRNKQLRALYSSIHPRAGV
                     GLGHKWSLHSRLGKGTTLEFMGEEKEDLIRHAKAILEKEDFDYFIFGHRHISMVYKLP
                     NGPEVVFLGEWVKNGSYAEWDGKNLTLIDFH"
     gene            complement(22587..23747)
                     /locus_tag="PK288_00385"
     CDS             complement(22587..23747)
                     /locus_tag="PK288_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM17403.1"
                     /translation="MKRSLTLLLLFLVIAGCQRKDMFTISGTIREKTKDVIYLSRLNV
                     NTLVLTDSSKVNKRGKFHFKARTKFPEFYHIGYSGQEFVTILAGPGEKIRINFNGNSL
                     PDNYSVSGSLGSEQVQMLEQRLAATKIRLDSLQQEYKKVSGQAGFEKTDSALSARYLE
                     LLKDLRRKNIEFIITNLKSMASIMALYQKTDENTYVLYDQRDLQYMKLVSDSLSTIYP
                     NSPNVKALTEDFKNELNRMYTRQIQNIAETKSEVRLDPNLEDINGRRIRLSSLKGKIV
                     LLTFWSAQSRECIEDNLQLKQLYGKYKNRGFEIYQINLDASEEVWRNGVKFDDLPWIS
                     TREDDPGNPANARVFNVRALPANYLFDRSGAIAGSNLFGRQLQIKLDQMLNN"
     gene            complement(23912..25075)
                     /locus_tag="PK288_00390"
     CDS             complement(23912..25075)
                     /locus_tag="PK288_00390"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM17404.1"
                     /translation="MSRKYLIFALAVILTGSCRNNTAEISGKIENPVPGEYLFLDELK
                     SNDLISVDSIMISGDGVFKFTRAVKYPSFYLLKINQKNYLTMLLTPGERIKIRTEHDS
                     LNFPSEISGSEGTELLVSYNRELLKAINRLKSLSDIYDRNIGKPTLPSVIDSLDLLAQ
                     SYLNEINRYTKEFIDRNITSLVSLMAIYQQVAPNVNVIDPSRDIAYFQKVDSSLYSRY
                     PHYDQVMIFHDKVAELTARYGEPGTDTGGPDKRMTAPEISLPSPAGDTIKLSSTRGSV
                     VLLDFWASWCPPCRTENPNLVYVYELYHKKGFQIYQVSLDKSKEAWIRGIREDHLEKW
                     IHVSDVKYWNSVVVPQYNIESIPFNYLLDREGRIIAKNLRGDALQRKLAELFK"
     gene            25303..27255
                     /locus_tag="PK288_00395"
     CDS             25303..27255
                     /locus_tag="PK288_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPM17405.1"
                     /translation="MSYIKFDKEQVVNLEYALSREVIRSNRAGSFSSTTIVGCNTRKY
                     HGLLICPVDAFGGERHVLLSSLDITVVFDDKSFNTGIRKYKGDYFSPKGHKYIEALEM
                     ETLPSRIYRVGSVILKQERMLVHYEEQYLVRITVLEASEPMKLQIRPFLAFRSIHELT
                     HANMACNTRVDYIPNGIKSKMYEGFPELHMQFSTQVEFVHVPDWYLGVEYPEEQKRGY
                     DYSEDLFTPGFFELEANEGDVIVFSASTREEKPSGLKAKFTKTVARKTPRNSFRHCLE
                     TAAQQFIERRNGKTYLIAGFPWFGSWGRDTFIALPGIALAKRNKLELYRDVLDTQVGR
                     MKDGLFPNMGNVGNPAFNSIDAPLWFFWAVQQYLMRGGNDAWLRYGNAMKDVINAYSH
                     GTEFNICMRDNGLIYADAPGKSLTWMDAVVNDVPVTQRKGYACEINALWYNAVCFTLD
                     LARKARDRKFVKEYEKLPELINKSFHEIFCSDEKGYIADYVNDEEGKNFFVRPNMVIA
                     VSMPYSMLSADEMKRILDVADKELVTPRGLRTLSPGNPKYKGTYSGNQEQRDNAYHNG
                     TVWPWLLGPFCEGWLKVYGKQGYQKVRKLIFGLEEVMSEHGVTTISEIYDGDPPHAPN
                     GAISQAWSVGEVLRVIDLLENEYANL"
     gene            27268..28572
                     /locus_tag="PK288_00400"
     CDS             27268..28572
                     /locus_tag="PK288_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM17406.1"
                     /translation="MRVLMFGWEFPPHISGGLGTASYGLTKGMSALEDLEVLFVVPKE
                     WGDETKTRVRLIGANNVPVAFKQIRYKGSKRPVEKIEVSSKIIPYTDPEEYWKKISSE
                     TTEHKFVIQTNDRGTVDFTGRYGSTLMEEIHKYAVVASVIAQENDFDIIHAHDWLAYP
                     AGIAAKEVSDKPLVIHVHATDFDRSGGNVNPEVYRIEKEGMDAASKIITVSNLTRDIV
                     INKYNIHPDKVETVYNAVEPVKMSQDVAVEKGFEDKVVTFLGRITMQKGPEYFIEAAN
                     KLLKVLPNVRFVMAGSGDMMERMMRRAAALKITDRFHFTGFLKGKDVFTMLHMSDVYI
                     MPSVSEPFGISPLEAMQSNVPVIISKQSGVSEILTHAVKTDFWDIDAMADAIYGILTY
                     PALAKMFIKNGKEEVVRLKWDNSARHVRDIYYRVIWQNKNKI"
     gene            28569..29768
                     /locus_tag="PK288_00405"
                     /pseudo
     CDS             28569..29768
                     /locus_tag="PK288_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
     gene            30072..34295
                     /gene="glgP"
                     /locus_tag="PK288_00410"
     CDS             30072..34295
                     /gene="glgP"
                     /locus_tag="PK288_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPM17407.1"
                     /translation="MNVDLVFETSWEVCNKVGGIHTVISTKALNFVNELRDNYILIGP
                     DVWREEATNPEFIPDDSLFMEWRSFAAAEGLVVRTGRWNIAGKPIVFLIDFTPFFGQQ
                     NEIFAGFWETYRLDSITGQWDYVEPALFGYAAGKIIESFTSFYRDRRDVIAQFHEWMT
                     GTGILYLKKYAPWIASVFTTHATVLGRCIAGNNKPLYGKMKEFNPVQISREFNVVAKQ
                     SLEKLSGSEADVFTTVSEITSRECAYFLGKEVDVVTPNGFEDSFVPSVEIFQEKRMEA
                     RKRLKKVAEAVLGYPVADNCVYVANSGRYEYRNKGVDIFIDALAELNTKDNLKKECIA
                     FILIPAYHRGPREDLIDVLNDNGQARGDEKYLTHYLHYPESDPILNNTKHKDITNSRE
                     SRVKIIFVPSYLNGNDGIFNMSYYDLLIGFDLTIFPSYYEPWGYTPLESLMFSIPTVT
                     TSLSGFGLWVKEYFNDPGNGMAVIERTDDNEAEVITSIKDFTKRFINLSDKEIEEARQ
                     KAHEISRIAMWDNLVRFYFSAYENALVHSRERREESREFAQFVETPGLQVRKPHQIPV
                     WKDIYVQSDVPDKLVSLKTLAGNLWWSWNTEAESLFRRMNPSLWEDVGHNPKLLLEKI
                     DYKRLLVLEDDDEFVADLDRVYSEFIRYMERPADPDKPSVAYFSMEFGIHQCLKTYSG
                     GLGILAGDYLKEASDSNYNITGIGLLYRFGYFKQKLGPKGEQLTIFEPEEFSRLPITL
                     IKDHEGRNLTVSIAWPGRTVNVRVWEASVGKVRLLLLDTDFEDNIPEDRSITHYLYGG
                     DHENRLKQELLLGIGGIRALTALGLKPDLYHSNEGHSAFISLERMRTMISENHLTFSE
                     ALEAIRSSTLFTTHTPVPAGHDAFEEDMLRKYISHYHTRFSISWDELMALGRCEGEAD
                     RKFNMSFLAAHMSQELNGVSKLHGQVTQGLFNKLWPGYLKEELYIGYVTNGVHYPTWL
                     APEWQPIYKELTGKVNFDQTDRSQWEKLYNLDDRRIYEIKKALKKKLFINIRKRLQTI
                     MIDKHVSPRTLMNISNHLDEKALTIGFARRFATYKRAYLLFRDLDRLARIVNNPDKPV
                     QFIYAGKAHPHDGGGQDLIKRVYEISQMPQFAGKVVFLENYDIELGKNLVRGVDIWLN
                     TPTRPLEASGTSGEKAVMNGTLHFSVLDGWWVEGYRAYAGWALPKKKTFTNQDLQDDI
                     DAETIYNMLEYEIVPAYYSFNDQGIPVEWISHIKNTMVKIAPEFTMKRMLDDYHEKYY
                     SKLWARNKYLTANNFEKAKEIAVWKKSILEKWDNIEVVNYHFERPEENVYHSGHDYRA
                     EIVLDIKNIPKENVGVEFVFTLLTNDGEYEFVDSQEFKLVSCKNGKCLYRADLIPEKA
                     GTFFYGIRIYPRHQDLPHKQDFYLLRWID"
     gene            complement(34292..36319)
                     /locus_tag="PK288_00415"
     CDS             complement(34292..36319)
                     /locus_tag="PK288_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HPM17408.1"
                     /translation="MNPSKYYLADPWLEPFTGIIESRINKCLEKERHLAGSRSLIQFA
                     MGHFYYGLHFTENSWVFREWAPNATRIFLIGTFSKWKEKQEFRLERINSRGDWEIKLP
                     SGSISHGDLFKLSVHWEGGRGERIPSYATRVIQDDRTKIFSCQVWKPESEYAWEIENF
                     TAPETAPVIYEAHIGMATEEEKLGTYAEFTGNVLPLIIKSGYNTIQLMAIQEHPFYGS
                     FGYHVANFFAASSRFGTPEDLKVLVDKAHQAGIRVIMDLVHSHSVKNINEGLGMFDGT
                     PGQYFHTNHRRNHIAWDSLCFDYGKDAVLHFLLSNCRYWLEEFRFDGFRFDGVTSMIY
                     YNHGLEKNFTSYNDYFDGGQDEDALVYLYLANKMIHEFKHGAITIAEEMSGMPGIACA
                     TDRSGFGFDYRLSMGVPDLWIKLVKDTADENWGMGLLGHELTQRRPEEKIISYCESHD
                     QALVGDKTLIFRMMDAEMYTGMNKPYHSFTVDRGIALHKMIRLITFFTAGGGYLNFMG
                     NEFGHPEWIDFPREGNNWSYKYARRQWQLAFNEDLKYSYLLKFDNSMVMLHKEFRILD
                     DMIVHRIYENDNDKVIAFSRGDFLAVFNFHPVMSFTDYGLPVKGKFRVVLDSDDPEYG
                     GFDRIDRLSDYSSTRKSLKQTLDQPFYLYLYLPSRTALVLRREAIRRATDI"
     gene            36428..37363
                     /locus_tag="PK288_00420"
     CDS             36428..37363
                     /locus_tag="PK288_00420"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPM17409.1"
                     /translation="MIIHTGTEKLDISNAVVTVGIFDGVHRGHRALLDYLVSYARRVN
                     GESVVVTFDKHPRMILSDSKSEIFYLSTLDEKKRLLADTGINHLVILEFNHSLAGMDA
                     GSFVKRILVGKIGIKHLIVGYDHHFGKNREGSYTLIRGYAERSGFNVEQIPGIASDHS
                     VISSTLIREALSNGNLRIASDMLGYEYSLAGKVIRGKGLGRDIGFPTANIEPDDMHKL
                     VPGSGVYAVRVSADDRLFLGMLSIGTNPTVNKDPDIRSIEVNIFDFEGDLYSREIRVY
                     FTERLRDEIRFDSIGQLKEQMKSDRLHALKVFSST"
     gene            37418..38833
                     /gene="ahcY"
                     /locus_tag="PK288_00425"
     CDS             37418..38833
                     /gene="ahcY"
                     /locus_tag="PK288_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPM17410.1"
                     /translation="MSIVAVDLQYKVKDITLADFGRKEIEIAEKEMPGLLAIRRKYSS
                     VKPLKGARITGSLHMTVQTAVLIETLVELGADVRWASCNIFSTQDHAAAAIAAKGIPV
                     FAWKGETLEEYWWCTEQALTFPGGKGPHLIVDDGGDASLLVHLGYKAENNPSILDKKP
                     AGREEGIIFQTLKNLLAKDPDKWHRTVRELTGISEETTTGVHRLYQMMENGELLVPAI
                     NVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVCGYGDVGKGCAKSMRSYG
                     ARVIVTEIDPICALQAAMEGFEVKTVEDALDEGNIFVTATGNRDVIRLDHMMRMKDQS
                     IVCNIGHFDNEIEVDKLNAMPGIVKTNIKPQVDKYTFQGGNEIFILAEGRLVNLGCAT
                     GHPSFVMSNSFSNQTLAQIELWKNRYEIGVYRLPKHLDEEVARLHLAQLGVKLTTLTR
                     EQADYIGVKIEGPYKPDHYRY"
     gene            complement(38856..39332)
                     /locus_tag="PK288_00430"
     CDS             complement(38856..39332)
                     /locus_tag="PK288_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17411.1"
                     /translation="MSTKLKIFFFLVIVTTSFFGRSCRHDENDVIPDVHVDFIIDLQD
                     PDFSNLMVAGSFDTINAKTRNWGQRAAGFNGNGIIVYAGIDGFHAYDRTCPYDYAISN
                     LSVRVKVDYSGAFCPVCGTKYALAAFAVPVSGTKAYPLKSYHTSLENDRYLRVWNK"
     gene            39413..39802
                     /locus_tag="PK288_00435"
     CDS             39413..39802
                     /locus_tag="PK288_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17412.1"
                     /translation="MNVLSGKRIFALVFVMGLAFSVVSGQGNKKYVRNPEKELFGKSL
                     NNKRPKIKEPGSVVRAKKKQEKARKRKEKEYAEYIKRNRARSLEIQTPEVRTRMKQNI
                     KEADTNFNNKRKKVEKESRAAARKYKK"
     gene            39940..40413
                     /gene="greA"
                     /locus_tag="PK288_00440"
     CDS             39940..40413
                     /gene="greA"
                     /locus_tag="PK288_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPM17413.1"
                     /translation="MSDVIYITEEGLNKLKEKLDHLRFVERPQISRQIADARDKGDLS
                     ENSEYAAAKEAQGMLEMKIARLENILTRSRIIDESKIDTSTVRILNKVRIKNLHNNSV
                     MEYQLVPESEANLKLGKIAVNSPIAMGLIGKKVGDLVDIKVPSGIISFEILSVSL"
     gene            40428..40826
                     /locus_tag="PK288_00445"
     CDS             40428..40826
                     /locus_tag="PK288_00445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPM17414.1"
                     /translation="MATIFTRIIRGEIPCHKIAEDERFFAFLDINPLREGHTLVIPKT
                     ETDYFFDLDDDTLAGLIVFSKKVAKAIREVIPCNRIGVAVIGLEVPHVHVHLVPMDSM
                     EDINFRNPKLRLSSDELNSLAEKISSKVNL"
     gene            40857..41558
                     /locus_tag="PK288_00450"
     CDS             40857..41558
                     /locus_tag="PK288_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPM17415.1"
                     /translation="MGYLYDLCDDFISLLFPRLCLGCGDHLLRNEKLICTRCYISIPR
                     TNFHLKSDNPVARLFWGRCHIEKAAAFSFYNRDSRIRKLIHQLKYKGEREIGFELGRI
                     YAFSLKSSGFLDGIDLIIPVPLHPLKERKRGFNQSEIISRGISDVSGIRVDVNSLKRT
                     GASDTQTRKSRYDRWTNVEGIFGMEDRGAVQGRHILLVDDVITTGSTVEACAEVLLRA
                     ENTVVCVVALAFAQV"
     gene            complement(41564..42130)
                     /gene="ruvC"
                     /locus_tag="PK288_00455"
     CDS             complement(41564..42130)
                     /gene="ruvC"
                     /locus_tag="PK288_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPM17416.1"
                     /translation="MKREQSERIILGIDPGTTTTGYGLIRIAGVGPELVAIGLIDLSR
                     FSDHYLKLKHIFDRVTGIIDEYQPCEMAIEAPFYGKNVQSMLKLGRAQGAAIAAALNR
                     SVHVSEYAPRKIKMSITGRGAASKEQVASMLMNILKFGDMEMKLDATDGLAVALCHFY
                     QSGAPSAEKTYHSWKDFLNRNPGRIKSR"
     gene            complement(42149..43459)
                     /locus_tag="PK288_00460"
     CDS             complement(42149..43459)
                     /locus_tag="PK288_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPM17417.1"
                     /translation="MKRPALIISVLLILIASCKKEPPVPPDDNEITPKQARDVLYTIM
                     NANYYWYDLMPSVNKEDYDGPEKLMAAMRYKPLDHWSFVADYDKFNAQMEGEFVGHGI
                     MIGIDDSSRARIALIYKEAPLYKEGVRRGWIVKSVNGTDVAPLIISGNTSEYNRVMGE
                     SKAGVTNTFVFRLPDGNEKTITSTKSSFILNSVLYFDTLQLKSGLAGHIVFDSFIATS
                     VDSLTKAFKFFNEQGINDLILDLRYNPGGFLLGAVALASFIAGNSVTANDLFIKLEFN
                     KKKTNENISYTFKNTPYPLNINHLVVITTSATASASEVVINGLEPYMNVITIGDTTTG
                     KCVGMGGWEYGKKYVFYPVTFKVVNKNNDSDYFDGLPPDIRVSDDITHDFSDRNELCL
                     KEAINYLETGSLGRKGIRPFTRQPHFSERPSWMSNSFLDPEAAK"
     gene            complement(43502..44425)
                     /locus_tag="PK288_00465"
     CDS             complement(43502..44425)
                     /locus_tag="PK288_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM17418.1"
                     /translation="MIRTAFCNDLNRMDRTRQSYIYAALSGLFWATVPTAFKICLGEL
                     PIIPMLTIASVTSLTILFILLLLTGKMNLLLECTVNDLAHSAILGLINPFLYYLILFR
                     AYQLLPAQVAQPLNMIWPIILVFLSVPLLKQKVKAKSYLALLISFTGVYLVSSQGAPL
                     RPGHSDTGGVLLAMGSSVFWALYFILNVKNRKDETVKLFLNFFFGAIYLITVLVITRQ
                     WSGDFSAKGIISAIYIGIFEMGITFWFWLKALKLATTTDKVSGLAYISPFLSLVFVHL
                     IIHEPIYYTTLAGLLLIISGIFVQNRNFGKG"
     gene            complement(44451..45725)
                     /gene="serS"
                     /locus_tag="PK288_00470"
     CDS             complement(44451..45725)
                     /gene="serS"
                     /locus_tag="PK288_00470"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPM17419.1"
                     /translation="MLTLNLIREKREFIIERLKIKNFDAEEIVDRVLALDASRREIQI
                     KTDSLQAEMNRISKQIGVLLKENRKEEAEAAREATYSLKDEIKALQDKAVPVEEELRN
                     EIIRLPNLPHDLVVPGHGAEDNVIVRQGGKTPELTTNALPHWDLIKKYDIIDFELGIK
                     LTGAGFPVYKGKGARLERALISFFLDEGVRAGYTEMMPPVLVNEDSGFGTGQLPDKEG
                     QMYHVGIDNFYLIPTAEVPVTNIYRDMILGSDQLPIKNIAYTPCFRREAGSWGAHVRG
                     LNRLHQFDKVEIVQITHPDHSYESLDEMVSHVESLVKALEVPYRIVRLCGADISFTSA
                     MTYDFEVWSAAQKRWLEVSSVSNFESFQANRLKCRFREKGDKQSRLVHTLNGSALALP
                     RIVAAILENNQEEKGIKMPAVLVPYTGFDYIS"
     gene            complement(45839..46099)
                     /gene="rpmA"
                     /locus_tag="PK288_00475"
     CDS             complement(45839..46099)
                     /gene="rpmA"
                     /locus_tag="PK288_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPM17420.1"
                     /translation="MAHKKGAGSSRNGRDSESKRLGVKLFGGQAVKAGNIIVRQRGTK
                     HNAGLNVGIGRDHTLFALVDGEVEFKKKKDNKIFVSVKPVSE"
     gene            complement(46372..46665)
                     /gene="rplU"
                     /locus_tag="PK288_00480"
                     /pseudo
     CDS             complement(46372..46665)
                     /gene="rplU"
                     /locus_tag="PK288_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            46957..47550
                     /locus_tag="PK288_00485"
     CDS             46957..47550
                     /locus_tag="PK288_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPM17421.1"
                     /translation="MKRVKLKVLGISYSQTQSGAYALILIEENGEKRIPIIIGGFEAQ
                     AIVIKLENLEPPRPLTHDLFKSFADKFDISLIEVMIYKLEEGVFYSRLLCNNGEKELS
                     IDSRTSDAVALALRFGCPIYINEDILDKAGITITSADTPAASESDDDHIYEAGDSKYR
                     NYPDDELQKMIDEAVRTEDYERAAAIRDELEKRKKKK"
     gene            47745..49694
                     /locus_tag="PK288_00490"
     CDS             47745..49694
                     /locus_tag="PK288_00490"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HPM17422.1"
                     /translation="MKIKYLYLLFVLMLPSHVIAGDYKVSSPDGRLVLTINAGTEILW
                     KASYDGKEIISSVKLGMILGDNKRFGENERIKRSITGKIDNILEPVVAVKHSVIPDQC
                     NTLHIIFRSGFAVQFRVYNDGAGYRFETSLKNEITVKEEIFSLAMPPGTHAWYPLEES
                     FMSHNERSYIYSTLDTITPHHLASLPVLFNTGGTNILLTEADIEDYPGLWITGDEKGK
                     LKGVHPGYPETEKLEGDRNLFVTSVRDYIAVTRGTRSYPWRVFVVAPTDRQLVESELV
                     YKLAAPVRLTDTRWIKPGKVAWDWWNATNIYGVDFRSGINTETYKYYIDFAAKNGLEY
                     IILDEGWYRIGGSVLESVPEIDVEELCRYGKEKNVGIILWVVFKTLWDDMDKAFDLYE
                     RWGVKGVKIDFMQRDDQKMVNFYHEAARKAAEHHLLVDFHGSYKPDGFGRTYPNALTR
                     EGVKGMENNKWSRDITPEHDVILPFTRMVAGPMDYTPGAMINKDVVNFNPQFTRPESQ
                     GTRVHQMALYIVFESPLQMLSDSPSNYMKEQECTDFIAGVPVVWDDLKVLDARVGDYI
                     LLARRSGQNWFVGGLTDWESRQMELDLSFLPAGEYMIDVFQDGINADKHAQDYKHLKY
                     NVSPADKIILKLAPGGGWAAKISPR"
     gene            49719..50513
                     /locus_tag="PK288_00495"
     CDS             49719..50513
                     /locus_tag="PK288_00495"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPM17423.1"
                     /translation="MKQYLDLLDHVLKNGIKKSDRTTTGTISVFGYQMRFDLQEGFPL
                     LTTKKLHMKSILHELLWFISGDTNIKYLTDNGVRIWNEWADKDGNLGPVYGYQWRSWP
                     AADGRKIDQLSNVIDSIKNSPDSRRHIVNAWNVGDLDKMALPPCHVLFQFYVAGGRLS
                     CQLYQRSCDIFLGVPFNIASYSLLTMMIAGITGLKPGEFIHTLGDAHIYLNHIEQVKL
                     QLTRKPYKLPSVLINPDVKEITQFRYDDFILVDYIAHPGIKGEISV"
     gene            50515..51027
                     /locus_tag="PK288_00500"
     CDS             50515..51027
                     /locus_tag="PK288_00500"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPM17424.1"
                     /translation="MAIISIIAAVSDDLGIGMKNELLWNIPEDLRRFKKLTMGKCIIM
                     GKRTWESLPRKPLPGRKNIVLTDVPEECIDCSVTAYSIEDALGKCGNDEEIFVIGGGS
                     VYRQFMPYADRLYITHVHRNSPADTWFPKIDMRKWRIFDKQECISGDDNMIPYTYVVY
                     ERKGRSQVTS"
     gene            complement(51148..52362)
                     /gene="clpX"
                     /locus_tag="PK288_00505"
     CDS             complement(51148..52362)
                     /gene="clpX"
                     /locus_tag="PK288_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPM17425.1"
                     /translation="MDKCSFCGRTKKEVSILIAGLEGHICDHCIEQAHSIMNEEVGSR
                     KNISFDTINLLKPAEIKNFLDQYVMGQDMAKKIISVAVYNHYKRLMQKPDADDVEIEK
                     SNIILVGETGTGKTLLARTVAKMLHVPFTIVDATVLTEAGYVGEDIESLLTRLLQNAD
                     YDVKAAEKGIVFIDEIDKIARKGDNPSITRDVSGEGVQQGLLKLLEGSIVNVPPQGGR
                     KHPEQKMIPVDTKNILFICGGAFEGIEKRIAQRLNTQVIGYGASRKRERIDKNNLLQY
                     IAPQDLRAYGMIPEIVGRLPVLSHLDPLDRKTLRDILTEPKNAIVKQYKKLFRMDNID
                     LTFGEGVLDFIVDKSIEYKLGARGLRAICEAIMLDAMFDAPSSGIQELTITTDYAIRK
                     LERIDMKVLKAS"
     gene            complement(52368..53045)
                     /gene="clpP"
                     /locus_tag="PK288_00510"
     CDS             complement(52368..53045)
                     /gene="clpP"
                     /locus_tag="PK288_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPM17426.1"
                     /translation="MNIFDDFGKYARGRYGISSLTMHKYSSILNSYISPTIIEERQLN
                     VASMDVFSRLMMDRIIFLGLPIDDYVANIIEAQLLYLDSSDPGKDIQIYFNTPGGSVN
                     AGLGIYDTMQYISADIATICTGMAASMGAVLLTAGTKGKRSALKHSRIMIHQPMGGFE
                     GQASDIEIQAREILKLKRELFEIIALHSGNPIEKVEKDSDRDHWMTSQEAKEYGMIDE
                     ILHKSEK"
     gene            complement(53109..54461)
                     /gene="tig"
                     /locus_tag="PK288_00515"
     CDS             complement(53109..54461)
                     /gene="tig"
                     /locus_tag="PK288_00515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPM17427.1"
                     /translation="MIISKENIDDLNAVLKVQVEKADYDENVEKVLRDYRRRANLKGF
                     RPGKVPYELIRRMYGRSVRIEEINKAVTENIQKYLADEKIDILGDPMPKTDEHEKIDF
                     DNQESFTFLFDLGLAPDFEIKLNKKNKVIGYEIEADEKMKNDYLETYRRRFGRFEKVD
                     LSEDKDLLRGKIEAIDNTSDPEYKGLFADDTTLGIDLIKDDDIKKEFIGKAPGDSIDF
                     DLRKALPNDYEIAGLLKRKKDEVESIDGRFRFTINEITRFKPAETGQELFDRIYGEGV
                     VGSEEEFRKRIEEEISANLKQETDFKLSSDIRALAIAKTEFSLPEDFLKRWLLKVNES
                     TTEEQIEKDFGSFRQDLKWQLIKNKIAKDNEVRITPEELQQEAENITRAQFRQYGLFY
                     AADEQIANYARETLKRSEDAKRIADKILDEKVLSLLKDLVRIDTKKVSIDEFNKLFEQ
                     "
     gene            complement(54538..54620)
                     /locus_tag="PK288_00520"
     tRNA            complement(54538..54620)
                     /locus_tag="PK288_00520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54584..54586),aa:Leu,seq:tag)
     gene            complement(54675..55400)
                     /gene="lptB"
                     /locus_tag="PK288_00525"
     CDS             complement(54675..55400)
                     /gene="lptB"
                     /locus_tag="PK288_00525"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPM17428.1"
                     /translation="MKLYTENLVKKYNNRTVVDHVSINVEQGEIVGLLGPNGAGKTTS
                     FYMIVGLIRPKEGRIFLDDEDITTLPVYKRARRGIGYLAQEASVFRNLSVEDNIRAVL
                     EMSGMPLSEQNERLETLLTEFGLHKIRRSKGIQLSGGERRRTEIARALALKPKFILLD
                     EPFAGVDPIAVEDIQEIVSKLKNKNIGILITDHNVHETLSITDRAYLLFEGRILMAGT
                     STQLAEDEHVRKVYLGKNFELRR"
     gene            complement(55420..56283)
                     /gene="tatC"
                     /locus_tag="PK288_00530"
     CDS             complement(55420..56283)
                     /gene="tatC"
                     /locus_tag="PK288_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPM17429.1"
                     /translation="MAKKGKSSRNNEGEMSFLEHLEELRWHLIRSILAIIVFMITAFI
                     LKNLIFDKIILAPKNPDFLTNRLLCRLGHLVNVDALCINSKPVNLINIKMAGQLTTHI
                     IVALVSGLIMAFPYIMWEFWQFFKPALHSNEKKYTRGAVVATSLLFFTGVLFGYFALA
                     PLSIHFLTNYQVSSDVVNQINIRSYIGTITSICLATGLVFELPIIAFFLTKIGVITPP
                     FMIKYRRHAIIVIFIVAAIITPPDVFSQMLVAIPLLALYEVGILISKRVMKKKEKEHK
                     EYLSADSEPAK"
     gene            56497..>58404
                     /gene="recQ"
                     /locus_tag="PK288_00535"
     CDS             56497..>58404
                     /gene="recQ"
                     /locus_tag="PK288_00535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HPM17430.1"
                     /translation="MVSDAAIHEYLKKYFGFDTFKGNQESIIKNVLEGRDTFVLMPTG
                     GGKSLCYQLPAVMKPGTAIVISPLIALMKNQVDAMRNFSTDDDVAHFLNSSLTKSESA
                     RVKQDVLSGKAKLLYVAPESLTKEENIDFLKNVDISFYAIDEAHCISEWGHDFRPEYR
                     RIRPIIDSIGRSPIIALTATATPKVQHDIQKNLGILEADVFKSSFNRPNLYYEVKSKQ
                     DVNREIIKYIKNNPGKSGIIYCLSRKKVEEMAENLKVNGIKALPYHAGMDSATRTANQ
                     DKFLMEEADIIVATIAFGMGIDKPDVRFVIHYDIPKSLEGYYQETGRAGRDGGEGNCI
                     AYYSYSDIVKLEKFMQGKPIAEQEIGKQLLLETVSYAESSVCRRKLLLHYFGEEYPKD
                     NCEACDNCLHPKAQFEGSEAVVNVLETVLAVKEKFKADHIAHILSGKITSAIKSYKHH
                     KLEYFGIGEDKDEKFWNMVIRQALIAKLLAKDIENYGLLKVTPKGMEFSEKPTSFMLA
                     EDHDYADPSDEDNAFGARTAAVDEELFSILKDLRKKISKQKEIPPFVIFQDPSLEDMA
                     IQYPINLDELQNISGVGAGKAQRYGKEFVEIIKKYVEEKEIIRPLDMVVKSVVNKSGI
                     KVYIIQSIDMKR"
BASE COUNT        16273 a        12479 c        13686 g        15966 t
ORIGIN      
        1 tacctgccga tgaattacgg tgagctttaa gacgcagggg agggcgttta atacagtctt
       61 tcgtttttcc aggggtcttt tatatattta ttgcttaaat atcccggcaa tgaacagagt
      121 tccgtcgttg gacgatataa gatccgcaca cctcaggata ctgccgtaca taaaccgcac
      181 tcctgttctt acctcaggtc agctgaacag gatttacggg tgcagccttt ttttcaaatg
      241 tgagaatttc cagaagacag gatccttcaa attcagaggt gcggtaaatg ccgttcttca
      301 gttgaatgag gaacagaaga agggaggggt ggttacccat tcttcaggga atcatgctgc
      361 ggccctttcg cttgcagcgc gaatgaatgg catcggcgca tatatcgtga tgccggaaac
      421 ctctcctgat gtgaagaaaa aagcagtggc cggatatggt gccgggatca ccttctgcaa
      481 acccggacag gcacagaggg aggcggcctg cagagaattg atgaatgaaa aaaaagcaac
      541 cctcattcat ccgtatgaca atttcaatgt tatttgcgga cagggtacgg cttcgcttga
      601 actgctggaa gaagtaaagg acctggatgt ggttgttgct ccggtaggag gtggtgggct
      661 gctgggcggc acctcctgct gcgtgaaggg aacggatggc aggatcaggg ttttaggagc
      721 ggagcccgtc aatgcaaatg atgcctggaa atcatttaaa accggaaaac taaccccttc
      781 tgttaatccg ttaacaatag ccgatggttt gctgacatca ttgagtgagc tgacatttaa
      841 tatcatcagg aatcattccg atgatatcat tacagtttcc gaaaagtcga taatagaatc
      901 aatgaaactg atatgggaac ggatgaagat aattgccgaa ccttcatcgg caaccgtact
      961 ggctgccgtc ggggagaacc ccggtcagtt taggggaaaa aggatcgggt tgatcatttc
     1021 aggcggaaat gttgatctga caagactgcc tttttagttt ttcttcctga aatatgcaat
     1081 gatcgggtaa tgatctgaat aattaatttt cattatatca aattgtctgc attcaagtcc
     1141 atttccatag agaatgtagt cgatcctgtt cgggggatag ttgcccctgt aggtgaatcc
     1201 tgcaccgtat cccgaagtca ggaaggaatc attcaaccct tttcggattt ttctgtatgt
     1261 aaatgaaacc ggagtatcat tgaaatcgcc ggcaactata accggataac ttgttttatt
     1321 tatcctgtct ttcaagatct gcgcctgtgc agcacgtctt ttatagcctt gcctcaggct
     1381 cctggagata cttctcagtt cactaacagc ctctttgtct cccgatgctt ctgttagctc
     1441 cgctgcaaac gatctttccc tcctttgcag cctgaaggat tccaggtgat tgttgaatat
     1501 cctgaaagtg tcgctttcga tgactatatc ggtaaaaatg gaaatgcttg acgaacgggg
     1561 atgatagatt tctcccctgt tgacgatcgg aaatcttgtc agagtggcga caccatagta
     1621 tcggttcctc ccggttgaaa taactttcag gtgtgaatgg tacctgttgc caagggccga
     1681 tctgagtgct gcctcttttc tttctggatc accctgaaaa taaagctcct ggagacatat
     1741 tatctccggc tggtggtttt tcagcaattc cattatcctc cgctccgacc tgaccggctt
     1801 tttgttttca taatagttga ataatctgac attgtaactc aaaacttcaa aggtgccggt
     1861 tttatcacct gagggtttgc ggagcctgat atagtttgag aaatgtgtca gacccattgc
     1921 aatgactgcg attgatatga aagcttcagc tttcaggaaa attatccata acagtgcaaa
     1981 caggatgttt ataagcagga ggtaggggta acttaacccc agtaatgccg gaaatgcaaa
     2041 tatgtcgggt ctgatatgca ccgaaatgta tgatgccaga agtgccaggg caaacagaat
     2101 gtttactgtc agcactattt tatatatcag ttttttcact taagcatatt aaatatgcac
     2161 gaaaataccg gattggcatt aaactaaatc aaaaaagctt gtttttttga caaggcagct
     2221 aaaattatgc ctgagcgtat tgaatcaatg gatcagtttt tattctggct ctcccggaaa
     2281 aggaaatctt tctcttcctt tgtgagagaa tcatatcctc cctttgatat cttgtcgagt
     2341 attatgttga tcttctcctg ccgttcagtc ttccttttgt tgtaatcata gtcgctgaca
     2401 ggttttttgt tggttaccct gatctttttt tctcttggtc tgagcgtggt aacaaaaaaa
     2461 tcgatcaccc ggttgatcca tttaccaagg tcccgtccac ggtggagatt aaccgcatac
     2521 aggtaaccaa acaatgctcc acccatatgt gcaagctttc cgcctgaatt cacggaaaaa
     2581 tccaggacag atgtgagtac aaatattgcc agggcaaggt attttatcct tattcttccc
     2641 aggagaagca ggctgacaga atagtcgggg acccaggctg ctattgagat cacgattgcc
     2701 attaccgagg ctgaggctcc gatggcaacg gaagtatcca ccactttgcc gaaaacagga
     2761 aaaatattga atgaaagaat atagaccagg gccccgctca gccctcccag caaatatacc
     2821 gccaccattt tcttctggtc gagatattcg agaaagatca gtccaaacca ataaagccag
     2881 agaagattga agaggatatg gagaacatcc ttgtgggtga acatataggt gatgagggtc
     2941 catggccttt tcaacagcga cgtgaatgag gaggggacag ccagtatgtt tacgattttt
     3001 gcctgcagtt cggggttgtc gaggaggaag gaaactattg atccggcagc gatcaggatg
     3061 aaaacggcga tatttatgta aatgagcctt gtaaggctcg ttccttttct gaatgaagcc
     3121 cttatggttt caaataaatg catcagtaca gggttcttgt tgtttttcgc cagtatctta
     3181 taaggaaata tccgaacagc atcccaccga ggtgagctgc gtgggcaata ttgcttccgg
     3241 gttgtgtgac tgcgaggtat agctcaatgg caccatagcc gagtacgaag tatttggctc
     3301 gtatgggaat cggaggaaaa agaagcatta gttgagtatt gggaaaaagg acaccaaagg
     3361 cgagaagtac accgaaaact gcccccgatg ccccgactgt cggaacatta agcagcatct
     3421 ggagtgtttt cattgtctcg tttgtaaata cctttccctg gctcagatag gatgcaccat
     3481 cattcaaaac cagctggagc tgatcagggc tgatgatacc cattacctta ttatactgga
     3541 acatgatcac cgtttcgtgg acaagagctg ctcccagtcc gcagattata taatagatga
     3601 agaaacgctt tggcccccag acattttcaa gtatgcctcc gaacatgaaa agcgcaaaca
     3661 tattgaaaaa cagatggagg aaattgccgt gcatgaacat gtgtgtaatg atctgtatcg
     3721 gcataaaatg ctccgatttt ggaaaataca acccaagtat catggtcagg tccgtactga
     3781 aaacactttt tgatgcccag ttagcaagca gcatcaggac atttaagagg atgaggtttt
     3841 ttacgaccgg tggtaacgcg aaaaagcttc ttccaaatcc gttcatctgt accagctttt
     3901 gtttgatggt tatttgtcca ggcaaagata ctattctcaa tgtatacggc aatacaggat
     3961 ttcttttctg ccgtctgcgg gaatttccta ataactcatt tcagcctttg ttcaatctcg
     4021 gccagatcga taatactgag aactggtttt cccagcgggg taaagttcgg cgaactgcag
     4081 gcaaagaggg tatcgaacat atcttccatt tcatccttga aaagaatcct tccgtgggga
     4141 attgaagaag ctgcagctaa agctgcggca actttgttcc tggttccttc tgaaggatct
     4201 gcaggactat ttttgtattc ctcgagaaat tgttccagca ttggtgccgg gtcgctgata
     4261 tttaagccgg ctgggagacc tgttataatg atcatgtcgg ggtcagcaag cctgaatctg
     4321 aatcctgtct tctggagatc ttcttcaatc tccctgatca gaaaataatc ggccgggtta
     4381 agatttatac tgacagggaa catcagtgcc tggctgagat ttgcaggacc tttgttctta
     4441 agtatttttt caaaaaggat cctttcgtgg gcccttttct gatcgatgat catcagcccc
     4501 gattttacgg ggcaggcgat gtatttgccg ccaagctgga agaaccttct ttctccttca
     4561 gtgtgaggag acttccgtcc gggaagatct gcggggggaa atagtttttc ccaatgggca
     4621 tgatgttcct tttcgaaatt atggatgaat tgctgcttat ggctgacaga attttcaccg
     4681 gcaaatggat catattgcct gttaattgct atttcaggcg gtacaggagt gtttcccgga
     4741 atggtgctta cggggatatc aatcagcatt tcgctgctga aatcgattga gggcacaatg
     4801 ttgaaccttc caagagcttc cttcacagaa gccataagga tttgccagat ggtccgttca
     4861 tcctcgaatt ttatctcggt ttttgtggga tggatgttaa tgtcgatcga tgcagcgtca
     4921 acttccatga agataaaata tgaagggatg gtttcagccg gcaggatgtt ctggcacgct
     4981 tcggtaacag ccctgtgaaa atagggatgt ttcatgaagc ggttattgac aaaaaagaac
     5041 tgctccccgt atgtgcgtcg tgcattttcg ggcttgccta catatccgct gacatttacc
     5101 agattggttg aagtatcaag cgggataaga tcattaccca tttgtctgcc gaaaactccg
     5161 acaatccgct gtcgccggtt tccggccgga aggctgtata tcacatcgtt gttatgcagc
     5221 agttgaaacc tgatttcagg atgggccagc gcgattttct ggaattcgat cacgatatgc
     5281 cgcatttcgg tgttgtcgga tttgaggaat tttctcctgg caggaatgtt gaaaaagaga
     5341 tttttaatac tgaagcatga gcccggcata cagctgcagg gttcctgtgc ctcaaccttc
     5401 gatccgttga taatgattaa tgttccttcg tcagtatctg ccttcctggt tctgagttca
     5461 accatcgaca cagctgcaat tgaggcaagg gcctctcccc tgaatccctt ggtctttatt
     5521 gcaaaaagat cattcgctga actgattttt gatgtagcgt gcctttcgaa tgacaacctt
     5581 gcatctgttt ccgacattcc tgtaccattg tcaatgacct ggataagagt tttccccgaa
     5641 tccctgatga tcagctctat attatcagcc cctgcatcaa cggagttctc cataagttcc
     5701 ttgactacgg acgcgggcct ctggatcacc tccccggcag caatctggtt tgcaacagaa
     5761 tcgggtaaaa gcctgataat atcagccatc agatggtata gttaatgccg ttgaaaatca
     5821 gcttttcgtt tacagcatac aaatatatat atttgcttaa ttaacactgg ttctcagata
     5881 cctgaacgtc actggtaaat tgctttaatt ttgtatcttt gaataaattc ctcaaataca
     5941 atgaaaaagt ttgtgttgtt agcatccctt ctgttgatcc tcctttgcgg ttgcaagagg
     6001 aaacccggtg aaaatgacct gaaggtgatt accctgaatg tcaggtacga caatcccggc
     6061 gacagtatga atgcatggcc caacaggatt cctttggtat tggaattctt cagggatcag
     6121 aaacctgacc tgatcggcct tcaggaagtt ttattccatc agtatgaatt gctcgattct
     6181 gtatggaaag attacagttc cgttggtgcg ggcagggacg acggtgcaaa gtcaggggag
     6241 atgaatccgg tatttttcag gaaagacaaa ttcgacctta taaggtcaaa aaccttctgg
     6301 ctttctgaag caccggagat tccgggttcc atggcctggg gagcgggcct tccgcggatt
     6361 gttacctggg tggagctggc cgacaaatcg acactcgaac atttttattt tttcaataca
     6421 cattttgcac atgattccga ttcagccagg ataatgagcg ctaaattact gttaagcaag
     6481 gtcgacagcc tggctgcagg attcccattc ataattacgg gcgacttcaa tatgctgcat
     6541 tcaagccaag gatattccat acttaccggt ccgtatgaaa gtgttccgct tttagccgat
     6601 tcttatgcca ttaccgacaa aaggcccgtg ggtccggcct atacctttca tggcttttcg
     6661 gaaaaatcga gtaccggaag gatcgatttc atctttgtca gaaatggtat gaaagtactg
     6721 gaacacaggg tgttcatcag gaaggaccat ggtatttaca tatcagatca ctggccggta
     6781 atggcagtgg tggaacttaa accctccagg cataaatagc cggcctgcct gctttccatt
     6841 tatcctccgg gctgattgtc ccggggacag gatgaaggat ccggaaagaa caattatttt
     6901 tttggtttat atcttgacgg ccaacccgga aagacgttta attttgcaaa gatcaggaaa
     6961 aacaaaaaat agcagattat gaaaacagaa ggcagacgga ccagcagcaa tattgaggac
     7021 cggaggggaa tgtcgaccgg taagatggct gttggcggag gaataggggc tatagccatt
     7081 gccgtgattg tgttgctgct tggaggcgat ccatcaacgg tacttgaaac gatgcaaagt
     7141 ccggctgttg agtcgggatc tgtgacatct tcccccgagg aggatgcaat ggctaatttt
     7201 gtttcggttg tgcttgcagg cacggaggat gtatggaccc gggtattcag ggagtcgaat
     7261 ctcacttacc gtcagcccaa acttgttctt ttcaggaacc aggtgcagtc tgcatgcgga
     7321 tatgcaagcg gtgcaagcgg acccttttat tgcccgggcg atgaaaaggt gtatattgac
     7381 ctggcatttt ttgatgagct cagaaccagg ttcggtgcgt acggcgattt tgccgttgct
     7441 tatgtggtcg ctcatgagat aggacaccat gttcagaacc tgctcggaat aatcaatgaa
     7501 gtgaacggta aacgggcacg acttgatcag gtgagctcaa ataagctatt ggtcaggctt
     7561 gagctgcagg ccgactttct tgcagggatg tgggcccatt atgaccagaa gatgtttaac
     7621 tcaattgagg cgggcgacat tgaagaagcc atgaatgcag ctgcttcggt gggtgatgat
     7681 gttctgcaaa agaggtacca ggggagggtg attccggatg cctttaccca tggctcagca
     7741 gcacagcgca aggagtggtt cagacgggga tacgtcaccg gtgactttga caagggagat
     7801 accttcaatg ccgatatttg aaaattgcat aaaatctgac gaaagccaca tggttggcca
     7861 aaaacatgta tgaatgaata ttgtcccgtt caatcagatg tgatttcctg ccggggcgtc
     7921 agacaggcat ccgaacgata aaaataaatt atatactggg aaaaaagaaa aaaatgtttg
     7981 acaggttatt tataataagc cttttagccg gtatgagcct gctgagttgc cgtacggcag
     8041 catctgagga ttcgcaggga tcggttttag tcaggaaatg tacagatttc actgttacag
     8101 gcgacgggaa gtccgaaaac tggagcagca ccaactggat catgcttggc aggcaggatc
     8161 agggaaaggt tgattaccgg accagggcaa aagtccttta ttcagaaaca gggatctatt
     8221 ttcttttcga ctgtgaagac aggaagctga cttcaacact gaaggcagat aatcttgacc
     8281 tatatactga agatgttgtt gaggtatttc tgtggaccgg ggaaaatttc ccggtgtatt
     8341 ttgaatatga gatttctccg ctggatttcg agcttccgat aataatacct aacaacaagg
     8401 gcacttttct tggatggctt ccctggaagt atgaagggga ccgcagggtc cggcatgcaa
     8461 cttccgtaaa gggaggcatg aaggaaagcg gtgctgctgt gaagggatgg atggctgaat
     8521 ttttcatacc ttacaggctc ctgacacccc ttggaaacgt acctccggta tcgggtacga
     8581 aatggagagc caatatgtac aggattgatt acgaccaggg gccaactcac ttttcatggc
     8641 agaaaacttc agggtcattt catgaatacc agaagttcgg cactttcatt tttgagtgaa
     8701 cctgtgtacc cttcatgtcc agtatgaagt aacaggtcca ggtccatacc atatctcaaa
     8761 ttacttttct ttttcataga aggatcaata tttaccattg gcggatctga cagtatctat
     8821 gttatcctgg attctggaag atcagttctt gtggagagat aaaaaaccgg tttcggtcgg
     8881 taaaatcccc ccgtacttct aataaatttt catgagctta cggcggcacc tatttttacg
     8941 ccaaaagctt atgtgtgaaa tccacatgat ataaaatcac aaacaagcat gaaaataaaa
     9001 ttcatgtggg ttccatgtga atcacttaat cttcagtagt gtataaattt attttcacat
     9061 gaaaaattta tttctttcaa tcttcatagc tgtacttctg ccgggcaatc ccatgaacgc
     9121 ccaggaagac aacgtctggt ctcttgaaga gtgcatcagt tatgcacttg agaagaacat
     9181 acaggtcagg aaaagcgaac ttgcaaatca gcggaaccag tattatgctg accaggccaa
     9241 agcccagaga tttccgtcgt taaacgcatc gctgaaccat aatttctcat ggagcaaaag
     9301 cgatggcgaa gggcaaacag ggtattcggg aattaatggt tcgaatgtct ccgtcaattc
     9361 tggggtcact cttttcaatg cttcacggat aacgaatcag ataaaacagg cagatcttga
     9421 tctccaggct ggtcaatatt ctcttgaaac agtaagggaa tccgttagtc tgagcattct
     9481 ggaggcttat ctgcaggtcc tttatgctga agaacaggta aagaacagta tcaggcaaat
     9541 tgaatcgact tccggccagg ttgacctggc agctgaaagg ctggcggtac agttaataag
     9601 ccaggcagat tatgcccagg taaaggcaca gcttgcgagc gagaaactca cccttgcaaa
     9661 ttcggagaac cagctggcca ttgcaaaggt caatctcgag caattgatgg aattgccggt
     9721 tagtgaaagc tttgccattg tgcatccaga attcggagaa caggttaacc agcacaggtt
     9781 gccggatgta atgttgatat atgaaacggc acttgggata aaaccccagg ttaaagctgc
     9841 agaggtgagc agacagattg cctcacttga cgaaaagatt gcaagggcag gatattttcc
     9901 gactctttca gcaagtgcag gtattggaac aagttattca agccggctga ccgatcccta
     9961 tttcagccag cttgataaag gattatcgcc atctctgggt ttctccctta caataccggt
    10021 ataccagaaa aaacaggtgc gcaccggcgt agctgtcgca aaagccggat accgcgatgc
    10081 tgaactgaat gagattgata ccaggaacgg gcttagaaaa aacattgaac aagcctgtgt
    10141 cgatgttacc tcggcccagg taaaatatga agcgagcctc gaaaaatata atgcaacaga
    10201 ggtatcggct gcattgtcgg atgaaaagtt caggcaggga ctgataaatt ccgtcgatta
    10261 tcttgtttcg aaaaccagtc tcattgtggc agaaagcgag cttttgcaag ccaggtacaa
    10321 tctgatcttc agctataagg tccttgactt ttaccaggga gttccacttg tattataata
    10381 attgttaaac aaatcaaata tcagactgat gatgaaaaag aaaattgtaa tagcagccct
    10441 gattacggcg gtactaacaa ttggagcagt tgttttgccg cctgtaatta aaaagaaacc
    10501 cgtttatacc ttcgatacgg caacggtaag ccgtggaacg atcacgaaca cggttactgc
    10561 aaccggtacc atagaagcca ccgttactgt tgaggtcgga acccaggtat cgggcatcat
    10621 cgaacatatt tatgtcgatt tcaatgataa tgtgaagcag ggccaggttc tggccaggct
    10681 ggatgagaca gcactcaggg ctcagcttga gcagagccag gcgaatgttg atcaggcccg
    10741 cgcccagctg aatttccagg aagccaccta caacaggctg aaagtactgt ttgaaaagaa
    10801 tcttattgca caggctgact atgatcaggc tcttttcaat tatgaaaatt cgaaggcatc
    10861 gttgagcaat gcacgttcag cccttgccag ggccgaggtg aatcttgcat atgcaacaat
    10921 atattcgccc attgacggcg ttgtcctgaa ccgggccata gaagaaggac agacagtggc
    10981 agccagtttt aacacccctg aaatgttcac aattgtcaat gatctcacgc aaatggaagt
    11041 gcagacaaac gttgatgagg ccgatattgg gaatgtaagg cagggccagc gggttgaatt
    11101 caccgttgat gcatatcccg atcttgtgtt cactggttcc gtttcccagg tccggctcca
    11161 gccggtaact acaaacaacg ttgttaccta tgttgtgatt ctgaatgccc cgaatcccga
    11221 gaaaaagctg atgcccggca tgacggcaag tgcaaccatc ttcatcgagg agaagcacaa
    11281 cacactggtt gtttcgggga aatcattgcg ttttactcct gatgccgggt acctgtcgga
    11341 gattatggcg aaggcttccg ggaaggttcc gaacaaggat tttcctgcag ttcctgtacc
    11401 agccgggatg gcaggtccgg caggcggagc gcccccggct ggattcgcca tgccaggatc
    11461 atcccgtgat ccgaaatcag ggatcgcaac attgtgggtg aaatcggaca agaatgtgat
    11521 cagtcccgtg ccggtaacca ttggcataga gaacggctcc aacgttgaaa ttctagatgg
    11581 gctggctgag ggagctgaag ttgtgatagc catgagcgga ggcgggaaaa aagagcagga
    11641 gaaccgtaac aggggcccga gaggtccttt cccgttctga tcattcagca gggtaatatc
    11701 taaaaccaaa gcagatggat aatacaatca tcaggatcac aaatctccgg aaagattatt
    11761 ttgtaggaga gattaccgtt catgcgctca ggggaattga catggagata aaggagggtg
    11821 agtttgttgc aatcatgggc acaagcggtt caggcaagtc gacaatgctg aacatactgg
    11881 gttgccttga tacaccatcg ggtggcagct atcatcttga ttcggtggat gtgagcacga
    11941 tgaataggga tgaacttgca aggcttcgca atcttaagct cggtttcgta tttcaggctt
    12001 acaacctgtt gccgaggaca accgcacttg agaatgttga attgcccctg ttttataata
    12061 accgcataaa ggctaaagaa cgcagggagc ttgccatgaa tgcactcgaa tcggtaggac
    12121 ttgccgacag gcttgatcat atgccgaacc agctttccgg cggacagcag caaagggtgg
    12181 cgatagcccg ttcactggta aacaatcctg tgcttatcct ggcggatgag ccaaccggga
    12241 atcttgatac acggacctca ttcgagataa tggaactttt ccaggacctg aactcaaaag
    12301 gcaggacaat cgtattcgta acacacgaat ccgacatagc caggttcgca acgcgcaaca
    12361 ttgttttcag ggatggcagg atacagcggg aagcctgtct gactgaacgg ctccttgcgt
    12421 ctgaaatact caaaagcctg ccagtggaag atattgagtt aacacaaccg aaataatcaa
    12481 tgaaacatgg atttgtcaaa tctttttaaa atagctgtca gggctcttgt aaggaacaaa
    12541 atgagggccg tgctgaccat gctcggaata atcatcggag tgggatcagt gatcgcaatg
    12601 ctggcaatcg ggcagggatc aaagcagaag ataagggacg aaatgtcatc gatgggtacc
    12661 aacatgatta tgataatgcc gaattatatg cggcggggcg gggtgagcct cggggcatct
    12721 tcctccatgg cactgaaata cagtgatgtt gcagcaatac gtaatgaggc ggctgcagtt
    12781 gccgctgtgt cgcccgaagt acgctcgaac aaccaggtaa tcttcggaaa ccagaatacc
    12841 cagacaacca tatatggggt gagtgaagag tatcttgcga tcaggaaact ggagatcagc
    12901 agcgggagga tctttacaca ggcggaaaca aggagcatgg caaaggtatg cctgcttggc
    12961 cggaccgtta ttgagaatct ttttggaaaa gacagtgatc cggttggcat gaccataagg
    13021 attaaaaacc ttccgtttga agtgataggc gtactcaaag gcaagggaga gaacggaatg
    13081 ggaatggacc aggacgacct gataattgct ccctacacaa ctgttcagcg gcgccttgca
    13141 gcaatagatt atatcaacgg gatctacgcc tcggcaacaa gcgaggccaa aagcgccgtt
    13201 gcaatagcag aggtggagga aatactgaga aggaatcata agctcaggga atcggaagaa
    13261 gacgacttca ggataatgtc gcagtcggag cttattgaaa ctttctcctc aataagcgac
    13321 gtgatgacct atctgctggg tgccattgcc gggatatccc tgctcgtggg agggatagga
    13381 ataatgaata tcatgtttgt ttccgtcacc gaacggacac gggagattgg tttgagaatg
    13441 tcaatcggag gaaggggcag ggatattctt cggcagttcc ttgttgaatc catgttatta
    13501 agtatcatgg gaggtacatt gggcgttatt tttggttata ttgtggcaaa agttgcagga
    13561 tcggtcatga acaacccggc aatcgtgaca tcacattcgg taatactggc atttgcagtg
    13621 tgttttgcca ttggagtatt ttttggctgg tatccggcaa agaaggcagc cggccttaat
    13681 cccatagaag cactcaggta tgaataaata caccttatga agaaaaaaat tgaagggaac
    13741 aaaattacga tcctcctgca tttccttgcc tgggtggtga tcatcgggat ccccctttat
    13801 tacattaggc gctggccttt cggaaaggac ttcatatgga ttaattatct gaataccctc
    13861 atcagcggta tgatattcta tgtgaattat ctgatccttg tgcccggttt gttcttcagg
    13921 acaaaaagat accggtatta tttgtcagta ctggctttga tagtgttgtt ctattttgtg
    13981 tccgacattg caaacaaaaa ggcgttttca tttgtaacgg aaaaagcccg gtcaggccag
    14041 gttgatataa aaccggaaga aaggggcaga atgatgccgc cgccgcccgg cggactgaga
    14101 ttacggtttc cttcacgtca tatatacctt ttcaattacg gattcaattc ggttttcctg
    14161 atttttttct ctcttggtct gagagttttt gaacgcaact ccaaaattga aaaacttcaa
    14221 aaggaaacag agcaggaaaa actaagatcg gagctggctt tcctgaagag tcagatcagc
    14281 cctcattttt ttttcaatac cctcaataat atctactccc tgataagcat caatgccgaa
    14341 gattctaaaa aagccgtgct gaagctttcg aagctgatgc gctatcttct gtacgaatcg
    14401 gaacagggaa cacccaggct gagcaccgag atcgatttta tgcagaacta tatcgacctt
    14461 atgaaactga gaatgagtga taaggtagat ttatcggttg attttccgga agtgtttgat
    14521 gatgtttcaa tcccacccct tctctttgtt ccttatattg agaatgcctt taagcatgga
    14581 ataagttaca gggagaaatc gtttattgcc atcaggcttg agcaggaagg tcagttgctg
    14641 aaattcacct gtaaaaacag catcattgcc cctgctgcca gaggagagaa ccgggagggc
    14701 cccggaatag gacttgagaa tgcggccaaa aggctgaatt tgctgttccc gtcacgacac
    14761 gagctgaaga tcagcaaaac agacaaggta tttgaggtat ttgttcaaat agacattcaa
    14821 tgaggatttc tgcaattgcc gtcgacgatg aaccactcgc cctgcagctt gtgatcggtt
    14881 acattgaaaa aacaccctgg ctcgagctgg caggaagttt tgacaatccg gtcgatgcaa
    14941 tggactttgt ggaggaacat aatgtcgatc tggtttttgt ggatattcag atgcctgatc
    15001 ttaacgggat tgagtttaca agatcggtaa agcgcagtcc gaaagtcatc tttacaacag
    15061 cctacgacaa atatgcaatt gaagggttta agctcgacat agtcgactat cttcttaaac
    15121 ctttcagtta tgaggagttc cttaaggcag tcaataaggc ccgccggctc ataatgctcg
    15181 aaaagggaac cgctgacaag attgaatcga gtaatgaatt ccttttcctt aaatcggaat
    15241 ataagatcag gcgaatagat ttcaatgata tactttacat tgaaggactg aaagattatg
    15301 tgaaagttta cctgatggat caggctaaac cagttatgtc gcttacaacc cttaagttgc
    15361 ttgagtcaaa acttcccgca tcaaaattca tgagggttca caggtctttc atcgttaacc
    15421 tcgaaaaagt ggacacgatt gaaagaggcc ggatcatatt cggtaaaacc tatattcctg
    15481 ttggtgaaca gtacaaagag aagttccagg gctttcttga caggaatttt cttgtttaga
    15541 aacctgtaat catgtagtat accccaaagt ggccggaatg aatgcaggat tttcattact
    15601 gtcctgcagc ctgttcagga acaatgtatt gccggcggtt acctggtgaa gtaaaccctg
    15661 tactcgcctt ctgacttttt atcgagccag tgtctgtact caaggctgag ggctttgtca
    15721 atcaggggag cggggataaa tggtgcaatg acttcaagta tctcatttcc gccgagcttt
    15781 ttgattgatg caagtacttc atgtaccggc tgttcatccc tgttgagcat ttccctgata
    15841 tcaattgatc tcacgatcat gtcgcctgtg aaccacccgg gacgatcggt aatgtatttt
    15901 gcaccgcctt catccatttg ttcgatgttc ccctggccaa cccgtgctct cagccggttg
    15961 acaagatctt caacctttaa ccccccgatt gtggcagcct ggcttatatt ggttatgcgg
    16021 gcaatggtct ttcgcagcac cgggttcctg agctttttaa attccggggc agcatcgata
    16081 agttcttcct ctaattcagg atatgagtgc agcagatcgt aaatttttgt tttaggggta
    16141 attatgagtt tttccatttt ctgttccttt tattcactgt aggataagat ccttttttca
    16201 ccttccagtt ttcgtgcatt ggaaagatcc tgcgatacct ccagggtccc caaatattca
    16261 ccgttctcgt ccctgagggc aaaatactcg atatgaatga atttccccct catctggatc
    16321 cagaatggcg cgtgagaagc ctttccggtt ttaaaatcgt cgatgatctt ttcaattata
    16381 tggacacttc ccggaggatg acagagtcgg acgtcacggt ttattattga ccggttcctt
    16441 gcaaaaatac gttccttacc ctgtgtgaaa tacttcacct tgtcattcct gtcaacgaat
    16501 gtcatgtcga agggtatggt gtttagtatt gccatgatct cggctgcggt aaaactgcct
    16561 gacgggagcc ggattgtgcc tgattcgtac gagatatcct cccctgtatt ttccgctgta
    16621 actccctccg gtttccagtc tgctttcgga tcatagagtg tatagccgaa ttcgagggtc
    16681 tgcctgtgta tctgccacca gtcgtcagtg gtaagaacat ccatgctcat gggaaaaaga
    16741 atatcttctt cctttttggt catgtcagcc atcgctttca gtaccggagc aaaaagaatt
    16801 tcagttgcat cctcaagatc agatttcctg agatcttcgc tcctgaggat ctctatgcag
    16861 cccttcagct gctccctgat ctcatcatgc ttgccccaca tgactttggg agggccggtg
    16921 atttcctgcc tttcaagata tggaaacaca aggtactcct tgcgtttgta gtgtttatcg
    16981 acatccatga gctcattgaa acacgacagt atctgaagag agtattccct gaaaccgctt
    17041 tccggaacag aatataacgt gccgagaagc atctgcgctt tttctattac cttaagcagt
    17101 tccctgtttt cctgcctgaa tacatccaca ggatgccctt cgggaataaa tgcggcgccg
    17161 gtctgatcga tatgcccgtc aagtacttct ccgtgaatgt cgcacagttt cagtatttcc
    17221 gtttcaggga ggccctcaga gatcagttcc tgttcaacct caacaacctc cccatacggg
    17281 acctgtctca atgtggctat aagttcctcc ctgacccttt gaggatcttc acctttatgc
    17341 aatttcagga taagttcctt cagtttttct ttcctgaact tcgaattatt tattaactca
    17401 ctcattttta atatattgtt tgtttaattt tatttacccc acttaatatt gccttctgtt
    17461 aattaattaa caatgcacac gggatattgt ttaaagagat cccttgtcat tgcttcatgc
    17521 tgaaacttgt gcaaaagtag cataagaatc taaggttgcc agtattttaa ttcatttgcc
    17581 tatttttata ctgacaacat aaaattttca tgaaatgcag gcagcttcgg tcactcatcc
    17641 tgagaacctc aacaatctga taaactttgc aaagcgcctg atcgacggcg aaaacggcag
    17701 ggagctcata ttgatgtaca aacattcgct ggaatcggtg acagctgagg aaacgatgaa
    17761 ggtgctggat catcttctga cttgcggata ttctttcagt acagtaaaaa acaatgtagg
    17821 caggatactg aatgcctttt tcagatctct taatgaccac aaatggaaaa ggccggctga
    17881 gcctcatttc ctgtattacc ttatgcttga aaacagggcg gtggagaaat taattgcagg
    17941 gatacgccct tcgttaaggc tgattctcgg tagggatgga acaggcagca gtgattcgct
    18001 cgttaaggtg agaaatttta taatgagctt aaaggaatac gaattacact atataaaaaa
    18061 ggagaatata cttttccctt atctcgaaaa aacattcaca caatatcgct gcctccagtt
    18121 aatgtggtct tttcatgatg atttcaggca gggtctgaaa accctggagg agatattgtc
    18181 agaagaagct gttgacaggg aaaggtttaa cagggaaatc gggaagttgt tttttgtgat
    18241 cttcccgatc atcttccggg aagaacagat tgtttttccg gttgcaatgg attcattacc
    18301 gacaggtgcc tggaatgaga tgctggcgca gagctttgag accggctggt gctacggaat
    18361 cagtccggcc gcgaaggatc agactgcggt tatgtcccgg aatgttgaca gggttgacct
    18421 tggaaccgga ttgctgaaac ccgggcaaat aatcctgatg ctggaaaatt taccggtcga
    18481 tattacattt gtggatgaaa atgatgaggt ttgttatttt tcagggtcaa aacacaggat
    18541 cttcccgcgt tcaaaagcca ttatcggcag aaaggttcaa aactgccatc cgcatgaaag
    18601 tgttcatgtt gtgaatgaga tactggaagc tttcaggaac ggctccaggg accaggccga
    18661 tttctggata cagtcgaagg gcaggtttat ccatatacgt tatttcgcaa tgcgcgatgc
    18721 agggggcagt tataagggga ctatcgaagt gagccaggat gttaccggga tcaggagcct
    18781 gcagggagaa aaacgacttc ttgactgggg ctgcctgact aaggataaat gaattacagt
    18841 actatcccag gtgagtgatc acagtcctgg ggctgcttgt tatccgtcgt gaagcttatt
    18901 gtatttccgc atttcagatt ttctcacttt atcagcgagt ctttattttc cgggtttatt
    18961 ttactatatt tgatagtgtg tttcctgaac gtgctgttaa ttaaaaatta tgttggttcg
    19021 tacatttgca agtgctgtta ccggcattga tgccggtacg gttaccattg aagttaatgt
    19081 gtcggttgga atacgttttt tccttgtcgg gcttccggat gttgcggtaa aggaaagcca
    19141 gcagaggatt gaatcggctc tgcggagcct gggttaccat tggccgggca ggcaggtggt
    19201 gatcaatatg gctccggcag acgtgcgcaa ggaaggctcg tcatatgacc tgccgctggc
    19261 agtgggaatc ctggcggcgg atgaaaagat tagccggggc aggctggagt cgtatgtaat
    19321 gatgggcgaa ctttcgctcg acggcaggct ccagcctgtg agaggcatcc tgccgatagc
    19381 cctgagggcc agggaggaag gattcagggg aattgtggtg cctgtcagaa atgcaaggga
    19441 agctgcggtg gtgaacggac ttgaggtttt tggaatggaa acccttggtg atgtggttga
    19501 cttttttaac gggaagcgtg atttctctcc cgtttcggtg aatctcacgg atctgtttaa
    19561 ccgggaagcg aacaaatatg atgttgattt ttcggatgtc aggggacagg aaaatgttaa
    19621 aagagcactt gaggttgcag cagcgggctc ccataacatt ctgatgatcg gccctccagg
    19681 atcgggaaag acaatgctgg ccaagaggat ttcgacaata attccgccat taacccttga
    19741 ggaagccctt gagaccacga agatccactc ggtcgcaggg aagatagatg atcatacggc
    19801 gctgatgata agaaggccgt tcagatcgcc gcatcataca atatcggatg tggccctggt
    19861 gggcggagga acattccccc agccgggaga aatttccctt agtcataacg gggtactgtt
    19921 tctcgatgag cttcctgaat tcaagcggac tgtgctggaa gtgatgaggc agccgctgga
    19981 agaccgggtg atcacgatat caagggcaaa atcgaccgtt gattatccgg ccagcttcat
    20041 gctcgtggct tcgatgaacc cctgcccgtg cggcttccat aatcatcctg aaaaggattg
    20101 catgtgctca acgggaatgg tacagaaata tcttaacagg atctcaggac cgcttctcga
    20161 caggatagat attcatattg aagttactcc ggtgaagttt gaaaaacttt cggatcaggg
    20221 caggattgaa aaatcagaaa cagtaaggga aagggtgatg aaagcaagga tgatccaggc
    20281 cagacgcttt aattcagtaa atggagttta tacaaacgca cagatgtcac cggcccagca
    20341 gaggaaatac tgccagcttg atgaggccgg aggaaaactg ctcagaacag cgatggaact
    20401 ccgcggcctt tcggccaggg cttacgaccg tatcctgaaa gtggcccgga caattgcgga
    20461 tctcgatgaa tcagaaaaca ttgccgtttc acatatatcc gaagcaataa attaccgtaa
    20521 cctcgacagg gagggatggg caggatgaac cttcatacag gactgacgga gaggacgatt
    20581 atttattctg aagtcagtcg gaaatttcat taatgcggac cgaaacggaa tatgcctgta
    20641 ctaatgtcag ggatttgccg ttatcattgt tgtaaacctt ttttgttcag gtttatattg
    20701 acaattattt atcgatgtat gtatggattt cattaattca atccattcga gaatggtgaa
    20761 tgaacgtctc ttgtttgcct accggggaga catttctgaa gagaattcat tgccgctgat
    20821 caccctcatc gaaaatgaaa tgaaaaccga gtcccacaga cttgctgtac ggaaaaagct
    20881 gttcatgttc gtactcgaaa gtattcagaa cataatcaga catggcaaca gggatcatgc
    20941 agacatgtca atggtaacct atgccaggac agatgatggc tactcaatca ccaccggcaa
    21001 catgatcgaa acggataatg tgaaggagct tgagaggagg ctggttcaga taaacaggtc
    21061 agatgaaagg gaaataaagt cgcaatacat gcagatattg aacagttcag agctgagcag
    21121 caagggcggg gcagggctcg gattgctgga aatggcaatc aagacgggga acaggcttga
    21181 ttatgatttt gttccctacg acgacaaata cacctacttt gttctgaaca aaaccgtcaa
    21241 tgcctctgga aagggaatta acaggggcgg gagaatgaac ggtgaaagaa aaggtttgct
    21301 ggacggagta gaacagttaa tggcagacaa caggatttat atgatctggt cggggcattt
    21361 ttcttccgat gtcggaggcg aggtactttc aattacagac gcgcggttaa ctaaaaatga
    21421 tctggaactc aggatcagaa aacgtatttt caccattcag atggagatac tggataatct
    21481 tgcaaaatat agtaccggcc gtgattcaga gaaaaaatat ggcctgccgg tcattatgct
    21541 aggattcgac ggactgaaat tcatcctgat ttccggcaat cttattgtta attcagcaat
    21601 accggaactg aaagcaaagc ttgataccgt gaaccggcac agtatcgatg aactcaggga
    21661 gatgttcaca cggtcattat ccgttcagac cacggataac gacagcaccg gtaacatggg
    21721 tctgattgac atagccagga aatcgggaag caagctgata tacagctttg aaaagattaa
    21781 tgaaacttat tcctatttct tccttacagt aaagaccgga acggacaatg atcagtgaaa
    21841 gtcaatgagg gtcagatttt ttccatccca ttccgcatag ctgccgtttt ttacccattc
    21901 gcctaaaaaa accacctcag ggccgttggg aagcttgtac accatggaga tatgcctgtg
    21961 gccaaaaatg aaataatcaa aatcctcctt ttccaggatt gctttggcgt ggcgaataag
    22021 atcctccttt tcctccccca taaattccag agtggttccc tttcccagtc gcgaatggag
    22081 cgaccatttg tgccccaatc caacaccggc ccgcgggtgt attgaagaat acagggcccg
    22141 cagctgttta ttgcggaata tccacaggag tattttgtat cctaaatcat ttgaaccgag
    22201 tccctcaccg tgggcgaggt aaaacctgcg gtttccgaat atcgttgaaa aaggcccggt
    22261 gtggcatatt actccacatt ccgtagacag gtaatctctg atccacatat catggttgcc
    22321 ggtaaagaaa tgaattacca cccccgagtc ggtcaaagcc gatattgtgc ctaggaaccg
    22381 ggtaaatcca cgaggtacaa ccagtttgta ttcccaccag aagtcaaaaa catccccaag
    22441 cagatagatc tccctcgctt caggtgctat tttcagtaac cattccacaa ccttcttttc
    22501 cctctcatgc ggatcttttc cagctttgag cccaaggtga aaatcggacg cgaaaaatat
    22561 tttgctgcgt tttttaatca taaggatcag ttatttaaca tctgatccag ctttatctgg
    22621 agctgcctgc caaagaggtt tgaccctgca attgccccgc tccgatcgaa aaggtagttt
    22681 gccggcagag ccctgacatt gaagacccgt gcatttgccg ggttgccggg atcatcctcc
    22741 ctggtgctga tccagggcag atcatcaaat tttaccccgt tcctccatac ctcctcgctt
    22801 gcatcaagat taatctggta tatctcaaat cctctgtttt tatatttgcc ataaagctgt
    22861 ttcagctgaa gattgtcttc tatgcattcc ctggactgtg ccgaccagaa agtgaggaga
    22921 actatctttc ccttcagtga cgataagcgt atcctccttc cgttaatatc ttccaggttc
    22981 ggatcaagcc tcacttccga ttttgtttct gcaatattct ggatctgcct tgtatacatc
    23041 ctgttcagtt cattcttgaa atcttcggtg agggccttga cattgggaga attcggatat
    23101 attgtggaca gtgaatccga aacaagcttc atatattgga gatcacgctg atcataaagc
    23161 acataggtgt tctcgtcggt cttctgataa agagccatta ttgaggccat tgatttgagg
    23221 ttggtaatta tgaattcaat atttttcctg cgcagatctt ttaaaagttc caggtatctc
    23281 gcactcaatg cagaatccgt tttttcaaat cccgcctgcc ccgaaacctt tttatactcc
    23341 tgctgcaggg aatcaagtct gattttcgtt gctgcaagtc tttgttcaag catctgaacc
    23401 tgttccgatc ccaatgatcc gcttaccgaa taattatcag ggagactatt tccgttgaaa
    23461 tttatcctga ttttctctcc gggccctgcc agaattgtca caaactcctg tcctgaatat
    23521 ccaatatggt agaattcagg aaattttgtc ctggctttaa aatgaaattt cccccttttg
    23581 tttactttcg atgaatcagt cagaaccagg gtatttacgt tcagccggct aaggtaaatc
    23641 acgtcttttg tcttttccct gattgttcct gaaatggtaa acatgtcctt cctttggcat
    23701 ccggcaatta caagaaacag caacagaaga gttaaagatc ttttcatccg tatataattt
    23761 tactttaggt ctgatgcccg cctgacccgt cgggcaggga accgcaaatg attgagcgcg
    23821 cctgtataca ttcgtgtttg tgtttttggt caatcatgtg gattccatca taaattaaag
    23881 tttgattgtt tgttgcattt tgattgtggg gctacttgaa tagttcggca agttttcttt
    23941 gcagtgcgtc tcccctcaga tttttggcaa ttatccgtcc ttccctgtcg agaagataat
    24001 tgaatggaat cgattcaata ttgtattgcg gcacaacaac cgaattccag tacttaacat
    24061 cgctcacatg gatccatttt tcgagatgat cttcgcgaat tccgcggatc cacgcctctt
    24121 tcgatttatc aagtgacact tgatagatct ggaatccttt tttgtgataa agttcgtaca
    24181 catatacaag attggggttt tctgttctgc atggcgggca ccacgatgcc cagaaatcca
    24241 gaagaaccac ggatcccctt gttgatgaaa gttttatcgt atcgcccgcg ggtgagggga
    24301 gtgaaatctc aggtgccgtc atacgtttat cgggtccgcc tgtatccgtt cctggttccc
    24361 cgtaccgtgc agtaagctct gcaaccttat cgtgaaaaat cattacctgg tcataatggg
    24421 ggtaccggga atatagtgat gagtcgacct tttgaaaata ggcaatatcc ctggaaggat
    24481 caataacatt cacatttggc gcaacctgtt gataaatagc catcagactg acgagtgaag
    24541 tgatgttccg gtcaatgaat tcctttgtgt atctgtttat ttcattgagg tatgactgtg
    24601 caagaaggtc gagggaatct atgaccgaag gcagtgtggg cttgccgatg ttccggtcat
    24661 aaatatcact gaggcttttt aaacggttga ttgctttgag aagttccctg ttgtagctga
    24721 caagcagttc agtgccttcg gaaccggata tttcagaggg aaaattcaac gaatcatgtt
    24781 ccgtgcggat ttttattctc tcacccggag tcaaaagcat tgtcagatag tttttttgat
    24841 taattttgag caggtaaaaa gaaggatatt ttacagccct tgtgaattta aagaccccat
    24901 cgcctgagat cattatggaa tcgacactaa tgagatcatt tgattttagt tcgtcgagga
    24961 acaggtattc ccccgggact ggattttcaa tctttccgga gatttccgca gtgttgttcc
    25021 tgcatgatcc tgttaaaatt accgccaaag caaaaattaa atactttcgg ctcatttaaa
    25081 aaattttaaa ttaaattgaa caataaatga aatgcaaagt tattcttttt ttatctgcat
    25141 gcttctttaa ataatatatt gatagtatgt cggacgacat tcgcaaaagt gattttcaat
    25201 gttttttcac ccttttgatt cctttgcggt gtcaaataaa aagattatct taaaagactt
    25261 ttagtatctg aagaaattaa tacctgaaca aaaaaatagt ttatgagtta cattaagttt
    25321 gataaggaac aggttgttaa tcttgaatat gccctttcca gggaagtgat ccggtcgaac
    25381 cgggcaggat ccttttcatc cacgacaata gtgggctgca acaccaggaa atatcatggc
    25441 cttctgattt gtccagtgga tgctttcggg ggtgaaaggc atgttcttct ctcatcgctt
    25501 gatataactg tagtttttga cgacaaaagc ttcaatacgg gcatcaggaa atacaagggt
    25561 gattatttca gtccgaaggg tcacaaatac attgaggccc ttgagatgga aacactgcct
    25621 tccaggatct acagggtggg cagcgtaatc ctgaagcagg agcgcatgct tgttcattat
    25681 gaggagcaat acctggtacg gataacggta cttgaagctt cagaacccat gaaacttcag
    25741 atccgtccgt tccttgcatt ccggagcatt catgaactga cccatgccaa catggcatgt
    25801 aacaccaggg ttgattatat tcccaacggg atcaaatcta aaatgtatga gggtttcccg
    25861 gaattgcaca tgcagttttc gacacaagtt gaatttgtgc atgttcctga ctggtatctt
    25921 ggagttgaat atcctgaaga acagaaacgg ggctatgatt attcggaaga tctttttact
    25981 ccgggctttt tcgaacttga agcaaatgaa ggtgatgtga ttgtattctc tgcatcaacc
    26041 agggaagaaa aaccttcagg cctgaaagct aagttcacaa agactgttgc gcggaagaca
    26101 cccaggaaca gtttcaggca ttgtcttgaa acagctgccc agcagtttat tgagagacgg
    26161 aacggaaaaa cctatttaat tgcaggattt ccatggtttg ggtcgtgggg cagggatacc
    26221 tttattgccc ttcccggcat tgcccttgcc aagcgcaaca agcttgaact gtacagggat
    26281 gtactcgata ctcaggttgg aaggatgaaa gacggcttgt tcccgaatat gggaaacgtc
    26341 ggtaatcctg catttaattc catcgatgct ccgctgtggt ttttctgggc ggtgcagcaa
    26401 tatctcatgc gtggcggcaa tgatgcctgg ctgaggtatg gcaatgccat gaaggatgtg
    26461 atcaatgcct acagccatgg caccgagttt aatatttgta tgagggacaa cggtttgatt
    26521 tatgccgatg caccgggtaa atcactgaca tggatggatg cggtagtaaa tgatgtcccg
    26581 gtgacgcaaa ggaaaggata tgcctgcgag atcaatgcat tatggtacaa tgccgtctgc
    26641 tttaccctcg acctggcaag aaaagcgaga gaccggaaat ttgttaagga atatgaaaag
    26701 ctgcctgaac ttattaacaa atcatttcat gagatcttct gcagcgatga aaaaggttat
    26761 atcgccgatt atgtaaatga cgaggaaggg aaaaacttct ttgtcaggcc caatatggtg
    26821 atcgcagtat ctatgcctta ttccatgttg tcggctgatg aaatgaaaag aatactggac
    26881 gttgctgata aggagctggt tactccaagg ggattaagga ccctgtcacc cggtaaccct
    26941 aaatacaagg gaacctattc aggcaaccag gaacagcgcg acaatgccta tcataacggc
    27001 actgtctggc cctggctgtt aggtccgttc tgtgaaggat ggctgaaagt gtacggaaag
    27061 cagggctatc agaaagtcag gaaacttatc ttcggactgg aggaggtaat gagcgaacat
    27121 ggcgttacca ctatttccga aatatatgac ggtgacccgc ctcatgcccc caacggcgct
    27181 atttcccagg catggagtgt tggcgaagtg ctgagggtga ttgatttgct tgaaaatgag
    27241 tatgcgaact tataattcag gaaaattatg cgtgttttaa tgttcggatg ggaatttcct
    27301 ccccatatct ccggcggcct gggcaccgcc agttatggtc tgacaaaagg tatgtcggct
    27361 ctggaagatc tcgaggtact gttcgttgta cccaaggaat ggggtgatga aaccaaaacc
    27421 agggtcaggc tcattggtgc caacaatgtt cccgttgcat tcaagcagat acggtataaa
    27481 ggctccaaac gacctgttga aaaaatcgaa gtatcatcta aaataatacc atatacagat
    27541 ccggaagaat actggaaaaa aattagttct gaaacaaccg aacataagtt tgtaatccag
    27601 accaacgaca ggggtaccgt cgatttcacc ggacgctacg gcagtacgct catggaggaa
    27661 attcataagt acgccgttgt tgcctcggtt attgcccagg aaaacgattt tgacataatt
    27721 catgcccacg actggcttgc ctatccggct gggatagcag caaaggaagt atcggacaaa
    27781 ccactggtaa ttcatgtcca tgccactgat ttcgacagga gcggaggaaa cgtgaatccc
    27841 gaggtgtaca ggatagaaaa ggaaggaatg gatgcggctt caaagatcat caccgtgagt
    27901 aatcttaccc gcgacattgt catcaataaa tacaatattc atcccgataa ggtggaaaca
    27961 gtctataatg ctgtcgaacc ggtgaaaatg tcacaggacg ttgcagtgga gaagggattt
    28021 gaagataagg ttgtaacctt tctgggcagg ataaccatgc agaagggacc ggaatacttc
    28081 attgaagccg caaacaagct gctgaaggtc cttccgaatg tcaggttcgt gatggcagga
    28141 tcgggtgata tgatggaaag aatgatgagg cgggcagccg cacttaagat aaccgacaga
    28201 ttccatttta cgggattcct gaagggaaag gacgtattta caatgctgca tatgagtgat
    28261 gtatacataa tgccttcagt atcagaacca tttgggatct ctccccttga agcaatgcaa
    28321 tcaaacgtcc ctgttataat aagcaagcag tccggagtat ctgaaatcct aacccatgca
    28381 gtaaaaacag atttctggga tattgatgca atggcagatg caatatacgg gatactgacc
    28441 tacccggctc tggcaaaaat gtttatcaaa aacggcaagg aggaagttgt gcgtctgaaa
    28501 tgggacaatt cagccagaca cgtcagagat atttattaca gagttatttg gcaaaacaag
    28561 aacaaaatat gagtgcagtt aataaaaaag caatttgctt ttattttcag gttcatcagc
    28621 ctttccggct taagagatac aggttctttg acctgggaca cgaccattat tattatgatg
    28681 atttctcaaa tgaatcaatc atgagaaagg tggcgaagaa ttgctatctt ccagccaatg
    28741 aagtaatcct ggatctgatt ttaaagaata aaggaaagtt caaggtttcc ttctcgctta
    28801 ccgggctggt aataaaccag tttcgccttt atgccccgga ggtgcttgaa tcattcagga
    28861 aactggccga taccggaatg gttgaattcc ttgcagaaac cgagtctcac tccctggctt
    28921 cgatcgtaac caggaatgag tttgaacggc aggtcaatgt tcacaaggca atgatcaggg
    28981 aatttctggg cgtggaagca acttcattca gaaataccga acttatttac tccgatcaga
    29041 ttggatcatg ggttgccgat atgggattca aatcgatgct tacggaagga gccaagcatg
    29101 ttcttggttg gaagagtccc aactttctgt actgcaataa tataaacccg agactcaaga
    29161 tattgctgcg taattttgta ctgagcgatg atattgcatt caggttctca aataaaaact
    29221 ggaatgaatg gcccctcacc gctgacaagt acgcattatg gctcaacaaa ctggcaccca
    29281 aaagtgaact gatcaatgtt tttatcgatt atgagacctt cggggaacat aacaagaagg
    29341 acaccggaat atttgacttc ctgcagcaca tgccgggagt tgtcatgaaa aaaacaccct
    29401 ataagttcat gacccctacc gaaatttccg attacctgca accagtcgct gcaataagtg
    29461 taccttctcc aatctcatgg gctgatgagg aaagggacat aacagcatgg cacggaaatg
    29521 aacttcaggt tgcggccctg gagaaacttt acagcctgtc tgataagatt aacaggtgcg
    29581 aggacgggca gattcaaaag gactggaaat acctgcaggc cagtgatcat ttttattata
    29641 tggccacaaa attcttctcc gacggtgccg tgcacgccta ttttaatcct tatgatacgc
    29701 cctatgatgc atttatgaac tatatgaatg tactaagtga ctttgaattg cgtttgaata
    29761 gatgtattcc cgatgttgcc gttcaaaatg aaatatcaaa actcaaccag ctgttgaggg
    29821 caaaggaaca gttaattgat cagcaggttg cagaaattga gtcacttaaa caccagcgaa
    29881 tgagaaaaag tccaaaaaag aatattaccg gccatatcga tctggttctt ccaaaagaaa
    29941 cgaaatccgg aaaaacaaga agccggaagc caaaagccaa aagcaattga tgaatcaggc
    30001 acgtgaacag acactcaata cgaatcctgc tcctgcttgc ttttctataa tctgtacaaa
    30061 tataaatcat aatgaatgtt gatctagttt ttgagaccag ttgggaagta tgtaataaag
    30121 ttggtggtat tcatacagta atctcaacta aggcacttaa ttttgtaaat gaactgagag
    30181 ataattatat acttataggc cccgacgtct ggagggaaga agcaaccaat ccggagttta
    30241 ttcccgacga ttccctgttt atggaatgga gatcatttgc tgcagccgaa ggattggtgg
    30301 tgaggaccgg aagatggaac attgccggga agccaattgt gttcctgatt gactttaccc
    30361 ctttctttgg tcagcagaat gagatttttg ccggtttctg ggaaacatac cggcttgaca
    30421 gtataacggg gcaatgggat tatgtggaac cggctctttt cggatatgcc gcaggaaaga
    30481 tcatcgaaag ctttacctca ttttatcgtg acaggcgtga tgttatcgca caatttcacg
    30541 aatggatgac tggtactgga atattgtacc ttaaaaagta tgctccatgg atagccagtg
    30601 tttttaccac tcatgccacg gttcttggaa gatgcattgc cgggaataac aagcctttgt
    30661 atggtaaaat gaaggagttt aatcctgtgc agatatcacg cgaattcaat gtggttgcaa
    30721 aacaatcgct cgagaaactc tcaggttccg aagcagatgt ttttactacg gtaagcgaaa
    30781 tcacatcacg ggaatgcgct tatttcctgg gcaaggaagt tgatgttgta actccaaacg
    30841 gatttgaaga ttcttttgtt ccttccgtgg agatttttca ggaaaaaagg atggaggcca
    30901 ggaaaaggct taagaaagtt gctgaagcag ttctgggata cccggtcgcg gataattgcg
    30961 tttatgtcgc caacagtggc agatatgaat acaggaacaa aggcgtggat attttcatcg
    31021 atgccctggc tgaactgaac acaaaggata accttaaaaa ggagtgcatt gcatttattc
    31081 ttattccggc ctatcacaga ggaccacggg aggatcttat tgatgtcctc aatgataacg
    31141 gacaagccag aggcgacgag aaatatctta cccattatct tcattatccg gaatcagacc
    31201 cgatacttaa caacacaaag cataaggaca ttacaaacag cagggaatca agggtcaaga
    31261 ttattttcgt tccgtcatat cttaatggta atgatggtat attcaatatg tcatattatg
    31321 acctgcttat tggttttgat cttacaatat tcccgtcata ttatgagcca tggggatata
    31381 ctcccctgga aagcctgatg ttctccattc cgactgttac aacttcactt tcgggcttcg
    31441 gactctgggt taaggaatat ttcaatgacc ccggaaatgg aatggctgtg atagaaagaa
    31501 ctgatgacaa tgaagctgaa gttataacct caatcaagga ttttacaaaa agattcatca
    31561 atctttctga caaggagatt gaggaagcca ggcaaaaggc gcacgaaatt tcacgtattg
    31621 caatgtggga taatcttgtt agattttatt tcagcgcata tgagaatgct ttggttcaca
    31681 gcagggaaag aagggaagaa tcgagggagt ttgcgcagtt tgtcgagact ccgggtctcc
    31741 aggtcagaaa gcctcatcag ataccggtgt ggaaagacat atatgtgcaa agtgatgttc
    31801 ctgacaagct ggtctccctg aaaacccttg cgggaaatct gtggtggtca tggaacactg
    31861 aagccgagtc tctttttcgc aggatgaatc cctcattgtg ggaagatgtg ggccataatc
    31921 caaaactgct tcttgaaaag attgattaca agcgtttgct tgttcttgag gatgatgatg
    31981 agtttgttgc tgatcttgac cgggtttact ccgaatttat ccgctatatg gaaagacccg
    32041 ctgatccgga caaaccctcg gttgcctatt tcagcatgga gttcgggatc catcagtgcc
    32101 ttaaaaccta ttcgggcgga cttgggatcc ttgcaggaga ttatcttaag gaagcaagcg
    32161 attcgaatta taatataaca ggtatagggc tactttacag gtttggttat ttcaagcaga
    32221 aacttggacc gaaaggggag cagcttacga tttttgaacc ggaggaattt tcacgtctgc
    32281 ctataaccct gataaaggat catgaaggtc ggaatctgac tgtcagcatt gcctggcccg
    32341 ggagaacagt gaacgtcagg gtctgggaag ccagtgtggg taaagtaaga cttctgcttc
    32401 tcgatacgga ctttgaagac aacattccgg aagatcgttc cataacacat tacctttacg
    32461 gcggagatca tgaaaacagg ctgaaacagg aactgttgct gggcattggc ggcataaggg
    32521 cactgactgc actcggactc aaacccgacc tttatcacag caatgaagga cattctgctt
    32581 tcatcagcct tgaaagaatg aggacaatga tctcggaaaa tcacctgacc ttcagtgagg
    32641 ccctggaagc cataagatca tccaccctct ttacaaccca tacccctgta ccggcgggcc
    32701 atgatgcctt tgaggaagac atgctcagga aatatatttc ccattatcat accaggttct
    32761 cgatatcctg ggatgaactg atggctctcg gccggtgtga gggagaagcc gaccggaagt
    32821 tcaacatgag cttcctcgct gctcatatgt cacaggaatt gaatggtgtc agcaaactgc
    32881 acggacaggt cacccaggga ttgtttaata aactctggcc cggatacctc aaggaggaac
    32941 tttacatagg atatgttaca aatggtgtcc attatccgac ctggctggct cccgaatggc
    33001 agccaatata caaggaactg acaggcaaag tgaatttcga ccagaccgac agaagtcagt
    33061 gggaaaagct ctataatctc gatgacaggc gtatatatga gataaagaaa gcgttgaaaa
    33121 agaagctttt tataaatata cgcaaaaggc ttcagactat tatgattgac aagcatgtca
    33181 gcccccggac attgatgaat atcagtaatc atcttgatga gaaggcgctt acaataggct
    33241 ttgcacgcag gtttgcaaca tataaaaggg cctaccttct tttcagggat ctcgacaggc
    33301 tggcaaggat tgtaaataac cccgataagc cggtccagtt tatttatgcc gggaaggccc
    33361 acccgcacga cggcggtggc caggatctta tcaaaagagt ttatgaaata tcacagatgc
    33421 cccagttcgc aggcaaggta gtcttccttg agaactacga cattgaactc ggtaaaaatc
    33481 ttgtcagggg tgtggatatc tggctcaata ctcctaccag acctctcgaa gcgtcaggta
    33541 caagcggcga aaaagccgtg atgaacggaa ccctccattt cagcgtactc gacggttggt
    33601 gggtggaggg atatcgcgct tatgccggat gggccctgcc caaaaagaaa acgtttacaa
    33661 atcaggacct tcaggatgat atcgatgcgg agacaatata taatatgctt gaatatgaaa
    33721 ttgttcccgc ctattactca ttcaatgacc agggaatccc cgtcgaatgg ataagccata
    33781 tcaagaacac aatggtaaaa atagcaccgg aattcacaat gaaacggatg ctcgatgatt
    33841 atcatgagaa atactattcc aaactatggg caaggaataa atacctgacc gccaataatt
    33901 ttgagaaggc aaaagaaatt gctgtctgga aaaaatccat tcttgagaaa tgggataata
    33961 ttgaagtggt taattatcat ttcgaacgac ccgaagagaa tgtgtatcat tcaggtcatg
    34021 attacagggc cgagatagtt cttgatataa aaaatatccc gaaagaaaat gttggggttg
    34081 agttcgtctt tacactcctg accaatgatg gtgaatatga gtttgtcgac agccaggaat
    34141 tcaaactggt cagttgcaag aacgggaaat gcctgtatcg tgctgacctg ataccggaaa
    34201 aagccggaac attcttctac ggcatcagga tttatccgcg ccatcaggat ctgccgcaca
    34261 agcaggattt ctatcttctg agatggatcg attagatgtc ggtagccctt cttattgctt
    34321 ccctcctgag aaccagggcc gtgcgtgaag gtaaatacag gtacagatag aaaggctgat
    34381 caagagtctg cttcaaggat ttccttgttg aggaataatc cgacaacctg tctatgcgat
    34441 caaaacctcc gtattcagga tcatccgaat caagcacaac cctgaacttg ccttttaccg
    34501 gcaatccgta atccgtgaat gacattacag gatggaagtt gaagactgcc aggaaatccc
    34561 cccttgagaa agcaatcacc ttatcattgt cgttctcata gatcctgtgt acaatcatat
    34621 catcgagtat cctgaattcc ttatgcagca ttaccattga attatcaaat ttcagcagat
    34681 aggaatactt gagatcttcg ttaaatgcca gttgccattg acgccttgca tatttgtaac
    34741 tccagttgtt cccttcacgg gggaagtcaa tccattccgg atgtccgaat tcattgccca
    34801 tgaaattcag gtagcctccg ccagcggtaa agaatgttat aagcctgatc attttatgaa
    34861 gggcaattcc tctgtcaacc gtgaagctgt ggtatggttt gttcatcccg gtatacatct
    34921 cggcatccat cattctgaat ataagggttt tatccccgac aagagcctga tcgtgcgatt
    34981 cacagtatga aattatcttt tcctcaggcc ttcgctgtgt gagttcatgt ccaagcaggc
    35041 ccattcccca gttttcatca gcagtatctt taaccagttt tatccacaaa tccggcactc
    35101 ccatcgaaag ccggtaatcg aatccaaatc cactcctgtc agtcgcacag gcaatacccg
    35161 gcataccact catctcctcg gcgatggtta tcgcaccgtg tttgaactca tggatcatct
    35221 tgttagctag atatagatag acaagggcat cctcatcctg ccctccgtca aaatagtcat
    35281 tatatgaagt aaagtttttt tcaagtccgt gattataata gatcatgctt gttacaccat
    35341 cgaacctgaa accgtcgaac ctgaactcct caagccagta cctgcagttg gaaagtaaaa
    35401 aatgaagcac ggcatccttt ccgtaatcaa agcacaatga atcccaggca atatgatttc
    35461 gtctgtgatt ggtatgaaag tactgccccg gggttccgtc aaacatcccg agcccctcgt
    35521 tgatattttt cactgaatgt gaatgtacaa ggtccattat cacccttatt cctgcctggt
    35581 gagccttgtc aaccagcact ttcaggtctt caggtgttcc aaacctggag gaggctgcaa
    35641 aaaagttggc aacatgatag ccgaatgatc cgtagaaggg atgttcctgg atggccataa
    35701 gctgaatggt attgtaaccc gattttatta tgagcggcag tacattcccg gtgaattcag
    35761 cgtaggttcc aagtttttct tcctccgttg ccattccgat atgtgcttca taaatgacag
    35821 gtgccgtttc tggagccgta aagttttcaa tttcccaggc atattctgat tcgggcttcc
    35881 atacctggca tgaaaatatt ttggtcctgt cgtcctgaat cacccttgtt gcataggagg
    35941 ggatacgttc tccccttcct ccttcccaat ggaccgaaag cttgaacaga tccccgtgac
    36001 tgatcgatcc ggatggcagc tttatctccc agtcaccacg ggagttaatc ctttccagcc
    36061 tgaattcctg cttctccttc catttgctga atgtaccgat aagaaaaatc ctggttgcat
    36121 tgggagccca ctccctgaaa acccagctgt tctcggtgaa atgaaggccg tagtaaaagt
    36181 gtcccattgc aaattgtatc agggacctgc tgcctgcaag atgtctttct ttttcaagac
    36241 atttgtttat gcggctttca atgataccgg taaagggttc cagccatgga tcagctaaat
    36301 agtactttga aggattcata cgggataaga tcttgaatgt cttttcataa aattacaaaa
    36361 acaaaatcat tcccggagtt ttattcgtct tcctttgcta attttgcatc cgcgaacaac
    36421 cagattaatg ataatacata ccggaacgga aaaacttgat atcagcaatg ccgttgtcac
    36481 agtcggcata tttgacgggg tgcacagggg ccacagggca ctgcttgatt atctggtcag
    36541 ctatgcacgc agggttaacg gggaatcagt ggtcgtaaca ttcgataaac atcccaggat
    36601 gatactctct gacagtaaaa gcgaaatatt ctatctgagc accctagatg aaaaaaaaag
    36661 gctgcttgca gacacaggga ttaatcatct ggtcatcctt gaattcaacc attcacttgc
    36721 cggaatggat gcaggcagtt ttgtaaaaag aatcctggtt ggaaaaatag ggataaagca
    36781 tctgattgtc gggtatgatc atcattttgg aaaaaacagg gagggcagtt acaccctgat
    36841 aaggggttac gctgaaagat ccggattcaa tgttgaacag atacccggga ttgcttcaga
    36901 tcattcagta ataagttcaa ccctgatcag ggaagcactg tctaacggca atctgcggat
    36961 agcctcagat atgctgggtt acgagtattc actggcagga aaggtcatca ggggaaaggg
    37021 acttggccgt gatattggtt ttcctacagc caatatcgaa ccggacgaca tgcacaaact
    37081 tgttcccggc agcggagtat atgcagtgag ggtttccgca gatgacaggt tgtttttggg
    37141 tatgttgagt atcggtacaa atccgactgt caataaggat cctgatatca gatcaatcga
    37201 ggtaaatatt ttcgattttg agggagatct ttatagcagg gagatcaggg tttattttac
    37261 tgaaagactt agggatgaga tacgttttga cagcatcggt cagcttaagg aacagatgaa
    37321 gtccgacagg ctccatgccc tgaaggtatt ttcgtcaaca tagttgatga attattgtta
    37381 actttgtaaa actgaatatt ttaaaactgg aagatatatg agtatagtag cggtggattt
    37441 acaatataag gtcaaggata ttactcttgc agattttgga agaaaggaaa ttgaaatagc
    37501 agagaaagag atgcccggac tccttgcgat cagaaggaaa tattcatccg tgaaaccgtt
    37561 gaaaggagca aggataacag ggtcgctgca catgaccgtg caaaccgccg tgctgattga
    37621 aactctggtg gaactgggtg ctgatgtgcg ctgggcaagc tgcaacatct tctccactca
    37681 ggatcatgcc gccgctgcaa ttgctgcaaa gggcattccc gtttttgcat ggaaggggga
    37741 aacccttgaa gagtactggt ggtgtaccga gcaggcgctg acattcccgg gaggcaaggg
    37801 gccgcacctg attgttgacg acggaggcga tgcatcactc cttgtgcatc tggggtacaa
    37861 agcggaaaat aatccttcga tccttgataa aaaacctgca ggcagggagg aaggaataat
    37921 attccagacc cttaaaaatc ttttggcaaa agatcccgat aaatggcaca gaaccgtacg
    37981 tgaacttacg ggaatctctg aagagaccac aaccggggta cacaggctgt accagatgat
    38041 ggaaaacgga gagttgcttg ttccggcaat aaacgtcaat gactccgtta caaaatcgaa
    38101 atttgacaac ctgtacgggt gtcgtgaatc acttgccgac ggtattaaac gggcaactga
    38161 tgtcatgatt gcaggcaagg tggtggtggt ttgcggttac ggcgatgtgg gaaaaggatg
    38221 tgcaaaatca atgagatctt acggagcaag ggttattgtt accgaaattg atcctatctg
    38281 tgcacttcag gctgcaatgg aaggctttga agtcaagacg gtggaggatg cacttgacga
    38341 gggtaatatt tttgtaactg caacaggaaa cagggatgtt atcaggctcg accatatgat
    38401 gagaatgaaa gatcagtcga tcgtttgcaa tataggtcat tttgacaatg aaattgaggt
    38461 tgataaactt aatgcgatgc ccgggatcgt aaagacaaac ataaagccgc aggttgacaa
    38521 atatactttc cagggcggaa atgaaatatt cattcttgcc gaaggaagac tggttaatct
    38581 tggctgtgca accggtcatc cgtcttttgt aatgagcaac tcattcagca atcagaccct
    38641 tgcccagata gagctttgga aaaacaggta tgagattgga gtttacaggc tcccaaaaca
    38701 ccttgacgag gaagtggcaa ggttacatct tgcacagctg ggagtgaagc ttacaacact
    38761 tacccgggaa caggctgatt acatcggagt taaaattgag ggtccctata agcctgatca
    38821 ttacaggtac tgatcccgat ctgctgaagg aaatcctatt tgttccatac cctgagatat
    38881 ctgtcgtttt caagacttgt atgatagctt ttcaaaggat aagcctttgt tcctgataca
    38941 ggtacagcaa aagctgcaag cgcatatttt gttccgcaaa cagggcagaa tgctccggaa
    39001 tagtcaactt taaccctgac actcagattg ctgattgcat aatcgtaggg gcaggtcctg
    39061 tcataggcat gaaaaccgtc aatccccgca taaactatga ttccgttgcc gttgaagccc
    39121 gcggcacgct gtccccagtt acgggttttt gcgtttatgg tgtcaaaact tcctgcaacc
    39181 atcaggtttg agaaatccgg atcctgcaga tcaataatga agtcaacatg cacatccgga
    39241 ataacatcgt tttcatcatg cctgcaggat ctgccaaaaa aggaagttgt cacaataacc
    39301 aaaaagaaga atatttttaa cttagtgctc atcggtgcaa cggaattgat gtaaaattaa
    39361 tatggtatgt agaaaaaaac aattcttgtc aatctaaaga agttatcaga tcatgaatgt
    39421 tttatcagga aaaaggattt ttgcactggt ttttgttatg ggtcttgcct tttctgtcgt
    39481 atcggggcag ggaaacaaga aatatgtcag aaatccggaa aaagaacttt ttggtaaatc
    39541 acttaataac aaaagaccta aaataaagga accgggaagt gttgtgaggg caaaaaagaa
    39601 acaggaaaag gcgcggaaaa ggaaagagaa ggagtatgct gaatatatta aaagaaacag
    39661 ggcaagatcc cttgagatcc agacgccgga agtgcgaacc cggatgaagc agaatattaa
    39721 ggaagctgac acaaatttca acaataaaag aaagaaagtt gagaaagaat ccagggcagc
    39781 agccaggaaa tacaaaaaat aaaccggaat agataatcgc ttgtataata ccacttttat
    39841 agattatttt ttggagaatc aataaaattt tcttaagttt gttctgaaga gttccgggat
    39901 tccccgggat tcttcttatt ttttaatcgg ggagaaaata tgtcagatgt tatttatata
    39961 acagaagaag gactgaataa gcttaaagaa aagcttgatc atctgagatt cgttgaaagg
    40021 ccccagatat ccaggcagat agctgatgcg agagataaag gcgatttatc ggaaaattca
    40081 gagtatgctg cagcaaaaga agcccaggga atgctcgaaa tgaaaattgc ccggcttgag
    40141 aacatcctga ctcgttcacg cataatcgat gagtcaaaaa tagacacatc cacggtacgg
    40201 atactgaaca aggtcagaat aaagaattta cataataata gtgtaatgga atatcagctg
    40261 gtacctgaaa gtgaagcgaa tctgaaactc gggaagatag ccgtcaattc ccccattgcc
    40321 atgggactca tcgggaaaaa agtaggtgat ctagtcgata tcaaagtgcc ttcaggaatc
    40381 atatcctttg aaattctttc tgtttcatta taatctgaat tatcagcatg gccacgattt
    40441 ttacacgaat cataagaggc gagatccctt gccacaagat tgctgaagat gagaggtttt
    40501 ttgccttcct tgacatcaac cccctgcgtg aagggcacac actggtgatt cccaaaacgg
    40561 aaaccgacta tttcttcgac cttgacgacg atacacttgc cggtcttatt gtattttcca
    40621 aaaaagtggc aaaggccatc cgggaagtaa taccctgcaa ccgcataggt gtggctgtaa
    40681 tcggacttga agtgcctcat gttcatgttc acctggtacc gatggattca atggaagata
    40741 ttaatttcag gaacccgaag ctacgcctga gcagcgatga attaaattca ctggcagaaa
    40801 aaataagcag caaggtaaat ctttaatatc tttgcatata taatccttct cgctgcatgg
    40861 gttatctgta cgacctgtgt gatgatttca tcagcctgct ttttccaaga ttgtgccttg
    40921 gttgcggtga tcatttgctc aggaatgaga aactcatatg caccagatgc tatatcagca
    40981 ttcccaggac caattttcac ctgaaatctg acaatcctgt tgcccgtctt ttctggggca
    41041 gatgtcatat tgaaaaagca gcggcctttt ccttctataa cagggacagc aggatcagaa
    41101 aactgatcca ccagctaaaa tacaaaggag aaagggagat aggattcgaa ctgggaagaa
    41161 tatatgcatt ttcattaaaa tcatcggggt ttcttgacgg gatagatctt ataattcctg
    41221 ttccgctaca tcctctcaag gaaaggaaac ggggcttcaa ccagagtgaa ataatctccc
    41281 gtggaatttc cgatgtctca ggaatccgcg ttgatgtcaa ctccctgaaa agaacaggtg
    41341 cttctgatac gcagacgcga aaatcgagat atgacagatg gaccaatgtt gaaggtattt
    41401 tcgggatgga agaccggggg gccgtgcagg gacgccatat tctgctggtg gatgatgtca
    41461 ttaccaccgg ctccacggtt gaagcctgcg cagaggtgtt gctcagggct gaaaatactg
    41521 ttgtgtgtgt tgttgcactg gcctttgcac aggtatgaag ctcctaacga ctttttatcc
    41581 ttccggggtt cctgttcagg aaatccttcc agctgtgata ggttttttcc gcagacgggg
    41641 ctcctgactg gtaaaaatga cataatgcga ccgcaagtcc gtcggtggca tccagcttca
    41701 tttccatgtc cccgaactta agaatgttca taagcatcga tgcaacctgt tcctttgagg
    41761 cagcacccct gcctgtgatt gacattttta tttttcttgg tgcatattcc gaaacatgaa
    41821 ccgagcggtt aagggctgct gcgatagctg caccctgggc cctcccgagt ttcagcatcg
    41881 attggacatt cttgccgtag aaaggagcct ctattgccat ctcacagggt tgatattcat
    41941 cgataatgcc tgttaccctg tcaaatatgt gcttgagctt cagataatgg tcactgaacc
    42001 tggaaagatc gatcagtccg atggcaacaa gttcgggacc aacaccggca atcctgatca
    42061 gtccataacc tgtagtggtt gtccccgggt caatcccaag tatgattctt tcgctttgtt
    42121 cccttttcaa tgcaggtgat taaaccggtt attttgcggc ttcaggatca aggaagctgt
    42181 tactcatcca tgagggtctt tccgaaaaat gaggttgacg ggtaaacggc ctgatacctt
    42241 tccttcccag ggatcctgtt tcaagataat taattgcttc cttcaggcac aattcattcc
    42301 tgtcgctgaa gtcatgggtt atatcatcgg ataccctgat gtcgggagga agtccgtcaa
    42361 aatagtcact atcattgttt ttatttacca ccttgaatgt caccgggtaa aaaacatact
    42421 tcttcccgta ttcccatcct cccattccca cgcacttccc cgtggttgtg tctccaatgg
    42481 ttattacatt catgtagggt tcaagtccgt ttattaccac ctcgcttgcc gaggcggttg
    42541 cagatgttgt tattactacg agatgattga tgttgagcgg gtaaggggta tttttgaatg
    42601 tgtatgatat attttcattt gtcttcttct tgttgaattc aagttttata aacaggtcat
    42661 tggcagttac tgaatttcct gctataaaac ttgccagggc cactgctccc aataaaaaac
    42721 ctccggggtt atacctgaga tcgagaatta gatcattgat tccctgttca ttgaaaaact
    42781 tgaatgcttt tgtcagggaa tcaaccgatg tcgctataaa cgaatcaaat acaatatggc
    42841 cggccagccc tgacttaagt tgaagggtgt caaaatacaa aactgaattg aggatgaagg
    42901 atgatttggt agaggtgatg gtcttttcgt tgccgtcagg tagcctgaac acgaaagtat
    42961 ttgtcacgcc tgctttactc tctcccatta ccctgttata ttcggaagtg ttgccggaaa
    43021 tgataagggg agcgacatcg gtcccgttca ctgattttac aatccatccg cgtcgcacac
    43081 cttccttgta taacggagct tccttgtata tcagggcaat tcttgcccgg ctcgagtcat
    43141 caatacctat cataattcca tgtccgacaa attctccctc catttgcgca ttgaacttat
    43201 cgtagtctgc aacaaaactc cagtggtcaa gaggtttgta ccgcatggca gccatcagtt
    43261 tctccggacc gtcgtagtct tccttgttta cggagggcat aagatcatac cagtagtaat
    43321 tggcattcat gatcgtataa agtacatccc gagcctgctt tggggttatc tcattgtcgt
    43381 caggtggaac aggcggctct tttttgcagg aggcaatcaa tatcagtaat actgaaatta
    43441 tcaatgccgg tcttttcatg tatcaaaatg ataatgtttc acattcagca aagttagcta
    43501 tttatccttt tccgaagttg cggttctgga caaatatccc tgaaatgatc agcaataatc
    43561 ctgccagagt ggtataatat ataggttcat gaataatcaa atgaacaaaa acaagcgaca
    43621 ggaatggtga aatataagcc agtccgctta ctttatcggt agtggttgca agcttcagtg
    43681 ccttcagcca gaaccagaat gtgataccca tttcaaagat cccgatataa attgcggaaa
    43741 taataccctt tgcagagaag tctccggacc attggcgagt gatgaccaga accgtgataa
    43801 gataaattgc tccgaagaag aaattcagga ataatttgac ggtctcatcc ttcctgtttt
    43861 ttacattgag tataaaataa agtgcccaga aaaccgagct tcccattgcc agcagtacgc
    43921 ctccggtatc tgagtggccg ggccgcaggg gagctccctg agatgatact aggtatacac
    43981 cggtaaaact gataagaaga gccaggtaac ttttagcctt aactttctgt ttcagcaggg
    44041 ggaccgacag gaaaacaagt attataggcc agatcatgtt gaggggttgg gcaacctgcg
    44101 ccggcagcag ctggtatgcc ctgaaaagga tcaggtagta aagaaatgga ttaatcagac
    44161 caagaattgc ggagtgagcc aggtcattta ccgtgcattc cagaaggaga ttcattttgc
    44221 ccgtcagcag gagcaatatg aagaggattg tgagggaggt gacggatgca atagtcagca
    44281 tcggaataat cgggagttcg cccaggcaga tcttgaaagc agtggggacc gtagcccaga
    44341 ataagccgga aagggctgca tagatataag attgcctggt tctatccatc ctgttcaagt
    44401 cgttgcagaa agccgtcctg atcatggaag gccttaaagg acagggttgc ttagctgata
    44461 tagtcaaatc cagtataagg aaccagcaca gcaggcatct ttataccttt ttcttcctga
    44521 ttgttttcaa ggatcgcagc cacgatcctg ggaagggcaa gtgcactgcc gttaagtgta
    44581 tggaccagcc gcgactgctt gtcgcctttt tccctgaatc tgcatttaag ccggtttgcc
    44641 tggaatgatt caaagttgga taccgaactt acctcgagcc acctcttctg ggcagctgac
    44701 catacctcga agtcataggt catggctgat gtaaacgaaa tatctgcccc gcagaggcgg
    44761 acgatccggt aaggaacttc cagtgctttc accaggcttt caacatgact taccatttcg
    44821 tcgagcgatt cgtatgaatg gtcgggatga gtgatctgga ctatttccac cttatcgaac
    44881 tggtgtagcc ggttcagccc cctgacatgc gcaccccagg aaccggcttc tctcctgaag
    44941 catggtgtgt aggcaatatt tttaatgggg agctggtcgc tcccaagaat catatccctg
    45001 taaatatttg taaccggcac ttcggcagtg ggaatcagat agaaattgtc aattcccaca
    45061 tggtacatct gaccttcctt gtcaggcagc tgaccggtgc caaatcccga atcctcatta
    45121 accagcacgg gcggcatcat ctccgtatat ccggccctga caccctcgtc aaggaaaaag
    45181 ctgataagtg cgcgttccag tctggcacct ttccccttgt aaacagggaa cccggctcca
    45241 gtcagtttaa tgccgagttc aaaatcgata atatcgtatt tctttatcag gtcccaatgc
    45301 ggcagggcat tggttgtcaa ttcgggagtt ttacctccct gcctcacaat aacattgtct
    45361 tctgccccat gcccgggaac caccagatcg tgcggcaggt tcggcagacg aatgatttcg
    45421 ttcctgagtt cctcctcaac cgggacagcc ttatcctgca gggccttaat ctcgtctttc
    45481 agcgaatagg tcgcctcacg agctgcttct gcttcctctt tccggttctc ttttaagaga
    45541 acaccgatct gtttcgagat cctgttcatc tccgcctgaa gggagtcggt ttttatctgg
    45601 atctcacggc gtgatgcatc aagtgcaaga accctgtcca caatctcttc ggcatcaaaa
    45661 tttttaattt tgagcctttc aattataaat tccctttttt cccggatcag attcagagtc
    45721 agcatagcaa ggtattaaag ttatttcaga acgatcagga acaaaaatac aaaactcccg
    45781 gaaatgcctt tccatcgggg aaaagaaatt cgggagccgt agttttttca gtgtactgtt
    45841 attcggaaac cggtttaacg gatacaaaga tcttattgtc ctttttcttt ttgaactcaa
    45901 cctctccgtc aacaagagcg aaaagtgtat gatcgcggcc aatgccgacg ttcagtccgg
    45961 cattgtgttt cgttccgcgc tgacggacaa taatattgcc agccttaact gcctggccgc
    46021 cgaaaagttt cactccaagc cgtttgcttt cagaatcgcg gccattgcgt gaacttcctg
    46081 ctcctttttt atgtgccatg ataaattatt ttattctgta tctttctatt cctttgattt
    46141 tgaagatttc ttaactgccg gtttcttttc ggccactttc tttgcagtag tcttcttccc
    46201 tttttcctca gctgtcccgg caggtttctc cacagctttt ttggatgtgc ccttcccggc
    46261 ggatgatttc ttttctgtaa cggttatttc ctcagccggc agctctgaag cacgttcttt
    46321 cacttttttc ggcgactgcc tgattaaatt tcccttttct ccgattcctg taatctcaac
    46381 ctgggtgaag ttttgccgat gcccgttttt taccctgtaa cccttctttc tttttttctt
    46441 gaaaacaata accttatctc ccttaacatg atccaataca tggccggcta caatataatc
    46501 cttgataaaa ggttctccga caagtatttt accaacgtct tcaaccagaa gtactttgtc
    46561 aaattcaata tcggcttctt tttcctccgg aagacggtgg acaaaaatcc ttttacctgt
    46621 ttctacttta aactgctgtc cggcaatttc aactatcgca tacattttat ttcttagatt
    46681 atgttattcc gggaggcgca tatgttgatg atcccatatg acttaaaccg ggccccgacg
    46741 gattctgttt gctgaattcg gactgcaaag atataaaaat tcaattactt tcaattaaaa
    46801 ttgcttgttt ttaataggga ttttgactga ctgactgcta tgaaaaagta ttgaaaaaaa
    46861 gaatggcttt tcagcttatc ttgtaactat tattatcttt atatttgtct gagataagat
    46921 gaaacctgat attcatctgg tttttaataa tttgatatga agagagtgaa gcttaaagtg
    46981 ttggggatct catacagtca gactcagtcg ggagcatatg cactcatact gatagaggaa
    47041 aacggagaaa aaaggatccc gataattatt ggaggatttg aagcccaggc aatagtaatc
    47101 aagcttgaaa atcttgagcc tccccggcct cttacacacg acctgtttaa aagcttcgcc
    47161 gacaaatttg acatttccct tatcgaggtg atgatttaca aacttgaaga aggagtgttc
    47221 tactcgaggc ttctctgcaa caatggcgaa aaggagcttt caatcgacag ccggacttcg
    47281 gatgccgttg cactggcact tcggttcgga tgtccaatat acataaatga agatattctc
    47341 gataaggctg gcattaccat aacctcggcc gatactccgg cagcatccga atccgacgat
    47401 gaccacattt acgaggccgg agattcaaag tacaggaatt accccgatga tgaacttcaa
    47461 aaaatgattg atgaagcggt caggactgaa gattatgaac gtgctgcagc catcagggac
    47521 gagctcgaaa agagaaagaa aaagaagtaa ttaccggttg cccggcgttg tggtttagtg
    47581 aaatcacttt ccccggattt cagatgttca tgcagccatt aataaaagtc acatgactga
    47641 taaataaaca caaacacgaa tgaatattat cacattcagt catgcatggt tccctgccag
    47701 gaatatccga caggcatccg tcctttaaaa ataattatat acatatgaaa ataaagtatt
    47761 tatatctgtt gttcgtcctt atgctgcctt cgcatgtaat tgcaggagat tataaggttt
    47821 catcacccga tggcagactg gttctgacga ttaatgccgg cacggagatt ctctggaaag
    47881 cttcctacga cggtaaggaa atcatctcat cggtaaaact gggaatgatc cttggcgaca
    47941 ataaaagatt cggggaaaat gaaaggataa aaaggagcat caccggcaaa attgataata
    48001 tccttgaacc ggttgttgcg gttaagcatt cagtaatccc ggatcagtgc aacacactgc
    48061 acataatttt ccgttccgga tttgcagttc agttcagggt atataatgat ggtgcaggtt
    48121 accggttcga aacatcattg aagaatgaga taactgtgaa ggaagagatc ttcagccttg
    48181 ccatgccacc gggcacccat gcctggtatc ccctggaaga gagtttcatg tcgcacaacg
    48241 aacggagtta tatctattcg acacttgata caataacccc gcaccatctt gcaagccttc
    48301 ccgttctttt caatacaggc gggacaaaca ttttacttac tgaggctgat atagaagatt
    48361 atccgggttt atggataaca ggtgatgaaa agggaaagct gaagggagtt catcccggat
    48421 atcccgaaac tgaaaagctt gaaggcgatc gcaatctgtt tgttacctct gtcagggatt
    48481 atattgcggt tacacgggga acacgaagct atccctggag ggtatttgtg gtggcaccga
    48541 ctgaccggca acttgttgaa agcgaacttg tatacaagct ggctgctcca gtgagactga
    48601 ccgacacaag atggataaag ccgggcaagg tggcctggga ctggtggaat gccaccaata
    48661 tttacggtgt cgatttcagg tcaggcatca atactgaaac gtataaatac tatatcgatt
    48721 tcgccgcgaa aaacgggctt gaatatatca ttcttgatga gggctggtac aggatcggag
    48781 gcagcgttct ggaatcggtt cctgaaattg acgtggagga attgtgccgt tatggcaaag
    48841 agaaaaatgt tgggatcatt ttgtgggttg ttttcaagac cctgtgggat gacatggata
    48901 aggctttcga cttgtacgaa agatggggtg tcaagggggt caagattgat tttatgcaaa
    48961 gggatgacca gaaaatggta aacttttacc atgaagctgc caggaaagcc gcagaacatc
    49021 atttactggt cgattttcac ggatcataca aacctgacgg atttggaagg acctatccta
    49081 atgccctgac cagggaagga gtaaaaggaa tggaaaataa taaatggagc agggatatta
    49141 ctccggaaca tgatgtcatt cttccgttta ccaggatggt tgcgggtccg atggattata
    49201 cgcccggagc aatgatcaat aaggatgtgg tcaattttaa tccgcagttt accaggcctg
    49261 aaagtcaggg aacccgtgtt caccaaatgg ctttgtatat tgtttttgaa agcccgctgc
    49321 aaatgctttc cgactctcct tcgaactata tgaaggaaca ggaatgcacc gacttcattg
    49381 ccggtgttcc cgtggtgtgg gatgatttaa aagtacttga cgccagggtc ggggactata
    49441 ttcttctcgc ccgtcgctcg ggacagaatt ggtttgttgg cggactaact gactgggaga
    49501 gccgtcagat ggaactcgat ctgtctttcc tgccggcagg agagtacatg atcgatgtat
    49561 tccaggacgg cattaatgct gataagcatg cgcaggacta taagcatctg aagtacaatg
    49621 tcagcccggc agataagata attctgaagc tggctcccgg aggcggatgg gcggcaaaaa
    49681 tatctccccg gtgaaaagga ttcttttgtg atacttgtat gaaacaatat ctcgatcttt
    49741 tagatcatgt ccttaaaaac ggaataaaaa aaagcgacag gaccactacc gggacaatca
    49801 gtgtatttgg atatcagatg agatttgacc tgcaagaggg tttcccgctc ctgaccacca
    49861 agaagcttca tatgaagtcc atactgcatg aacttctatg gtttatttca ggcgatacaa
    49921 atattaaata tcttaccgat aacggtgtaa ggatctggaa tgaatgggcc gacaaggatg
    49981 gcaatctggg cccggtttac ggataccagt ggcgctcctg gccggctgca gacggaagga
    50041 agatcgacca gctcagtaac gttattgact cgataaaaaa ctcacctgat tcacggaggc
    50101 atattgtaaa tgcctggaac gttggcgatc tggataaaat ggcacttccg ccatgtcatg
    50161 tcctgttcca gttttatgta gcaggtggaa gactttcatg ccagctgtat cagcgaagct
    50221 gtgatatttt tcttggtgtg ccctttaata ttgcatccta ctcactgctc acaatgatga
    50281 tagcaggaat taccggactg aaaccggggg aattcatcca tacccttggg gatgcacata
    50341 tttacctgaa ccatattgaa caggtcaaac ttcagcttac acgaaaacct tacaaacttc
    50401 cctcggtttt gataaatcct gatgtgaaag aaataacaca attcaggtat gacgatttta
    50461 ttctggttga ctatattgca catcccggca ttaagggaga aatatcagtg tagaatggcg
    50521 ataatatcta taattgcagc tgtttccgac gatcttggga tcgggatgaa aaatgaactt
    50581 ttatggaata tcccggaaga tctcagaaga ttcaaaaagc tcactatggg caaatgtata
    50641 attatgggca aacgaacctg ggaatcgctt cccagaaagc cgttgcccgg acggaaaaat
    50701 atagttctga cagatgttcc ggaagaatgc attgattgtt cggtgacagc ctattcgatc
    50761 gaggacgcat tgggcaagtg cggaaatgat gaggagatat tcgtgatcgg cggcggatcg
    50821 gtatacagac agtttatgcc ctatgcagac cggttgtaca taacccatgt tcacaggaac
    50881 tcacctgctg atacctggtt cccgaagata gacatgagaa aatggcggat tttcgataag
    50941 caggaatgca tatcgggaga tgacaacatg attccataca catatgttgt ttatgagagg
    51001 aaaggcagaa gccaggtgac ttcttaaaag acctcacaaa agattactga taagacgtct
    51061 tttataatct tcttgagaat atgcagcatt ctggcagatg ttatggtttc agtttaaatg
    51121 aggatacccg atgccagacc ggtcagccta tgatgcctta agaaccttca tgtcaatcct
    51181 ttcaagcttc cggattgcat agtctgtagt tatggtaagt tcctgtattc cactcgaggg
    51241 agcatcgaac attgcatcca gcattattgc ttcgcagatt gcccgcagcc cccttgctcc
    51301 aagcttgtat tcaatggact tatcgacaat gaaatcaagg actccttctc cgaatgtaag
    51361 gtcgatattg tccatccgga acaatttttt atactgtttt acaatggcat tctttggttc
    51421 agtgaggatg tccctcaggg ttttcctgtc aagcgggtca agatgtgaga gcaccggaag
    51481 gcgtccgaca atttccggga tcattccgta tgcccgcaga tcctgaggag cgatgtactg
    51541 aaggagattg tttttgtcaa tcctttctct tttccgggag gctccgtagc ctattacctg
    51601 ggtattgagc ctttgtgcga tccttttttc aattccctcg aatgccccgc cgcagataaa
    51661 aagaatattc ttcgtgtcga cgggaatcat tttttgttca ggatgcttgc gtccgccctg
    51721 gggaggtaca tttactatag atccttcaag gagtttcagg agcccctgtt gtactccctc
    51781 tcccgaaaca tcgcgtgtta tggacggatt atcgcctttc ctggcaattt tatcaatctc
    51841 atcgatgaaa acaattcctt tttcagctgc cttcacgtca taatcggcat tctggagcag
    51901 tcgggtaaga aggctttcta tgtcttcccc cacatatcct gcctctgtaa gcaccgtagc
    51961 atccacaatg gtgaaaggaa catgcagcat tttggcaacg gttcttgcaa gaagtgtctt
    52021 gccggtacca gtttccccaa ccaggatgat atttgatttt tcaatctcaa catcatcagc
    52081 atccggcttt tgcataagcc tcttatagtg gttgtaaaca gcaactgata tgattttctt
    52141 ggccatatcc tgccccataa cgtactggtc gagaaaattc tttatttctg caggtttgag
    52201 caggtttatg gtatcgaatg aaatattctt cctggagccg acttcttcat tcattatgct
    52261 gtgagcctgc tcaatgcagt gatcacagat atgtccttca agtccggcaa taagaatgct
    52321 gacctctttt ttggtacgtc cgcaaaatga gcacttatcc attcaggtta tttttcgctt
    52381 ttatggagta tctcatcaat cattccgtat tctttagcct cctgtgaggt catccagtga
    52441 tccctgtccg agtctttttc aactttttcg atcgggttgc cggaatgtaa ggctatgatt
    52501 tcaaacagtt cccgtttaag tttaaggatt tcacgtgcct gtatctcgat atctgaggcc
    52561 tgtccttcga atccgcccat aggctggtga atcattatcc tcgaatgctt aagggccgat
    52621 cttttgccct ttgttccggc tgtaagaagt accgctccca ttgacgcggc catgccggtg
    52681 cagattgttg caatgtcggc agatatgtac tgcatcgtgt cataaatacc aagtccggca
    52741 tttaccgacc ctcccggcgt attgaaataa atctggatgt ccttgcccgg atcggaagag
    52801 tcgaggtaaa gcaactgggc ctcgattata tttgccacat agtcatcaat tggcaggccg
    52861 agaaaaataa tcctgtccat cataagacgc gaaaacacat ccattgatgc cacgttgagc
    52921 tgcctctcct ctatgatggt tggtgaaata taactgttaa ggatcgagga atatttatgc
    52981 attgtcaggc tgcttatacc ataccttccc ctggcatatt ttccgaaatc atcaaaaatg
    53041 ttcatgttga aaatgttaag ttttgcatac aaagatatga aaaatagtaa aatccggtac
    53101 gatggccctt actgctcaaa tagcttgttg aactcgtcaa tggacacttt tttggtgtcg
    53161 attctgacca gatccttcag caggctcaga accttttcgt caagtatttt gtcagcaatt
    53221 ctttttgcat cttcactgcg tttaagggtt tccctggcgt aatttgcaat ctgctcgtct
    53281 gccgcgtaaa agagaccgta ctgcctgaac tgggccctgg taatgttttc agcttcctgc
    53341 tgcaactctt cgggagtgat tctaacttcg ttgtctttgg caatcttatt cttgataagc
    53401 tgccatttaa gatcctgcct gaacgacccg aaatccttct ctatctgttc ctcggtagtt
    53461 gattcattta ccttcagcag ccaccgcttg agaaaatcct ccggcagact gaattctgtc
    53521 tttgcaatgg ccagggccct gatgtctgaa gacagcttga aatctgtttc ctgtttaaga
    53581 tttgcactaa tttcttcctc tatgcgtttc ctgaattctt cctctgagcc gacaactccc
    53641 tcaccgtaaa tcctgtcaaa cagttcctgc cctgtttcag ctggtttgaa gcgggttatt
    53701 tcatttattg taaacctgaa ccggccgtca attgattcca cctcatcctt ctttcttttc
    53761 agaagccctg caatttcata gtcattgggc agggctttcc tgagatcgaa atcgatggaa
    53821 tcacctggag cttttccgat aaactctttt tttatatcat catctttgat cagatcgatt
    53881 cccagggttg tgtcgtcggc aaacaaacct ttgtattccg gatcagatgt attgtcaatg
    53941 gcttcgatct ttcccctcag cagatcttta tcttccgaca gatcaacctt ttcgaatctt
    54001 ccaaacctgc gtctgtaggt ttcgaggtaa tcattcttca ttttttcatc cgcctctatt
    54061 tcataaccga taaccttgtt tttcttgtta agttttattt caaaatcggg tgcgagcccc
    54121 agatcaaaca aaaaagtaaa ggactcctgg ttgtcaaaat caattttttc atgttcatcc
    54181 gttttgggca tgggatcgcc gagtatatcg atcttttcat cggcaaggta tttctgaata
    54241 ttttcggtca cggccttgtt gatctcctca atccttaccg acctgccgta cattcttcta
    54301 atcagttcgt atggtacctt gcccggacgg aaacctttca ggttcgccct cctgcgataa
    54361 tccctcaata ctttttcaac attttcgtca taatcagcct tttcaacctg aactttaaga
    54421 acagcattaa ggtcatcgat gttttccttt gaaataatca ttttatatct gtttatggca
    54481 tttaaaaaaa ccgctgaacg catctcggat gatcgttacg gacggttcgc aaatctgtgc
    54541 ggatgaaggg actcgaaccc ccacgtcact aggacacaag atcctaagtc ttgctcggct
    54601 accaattacg acacatccgc aaaaaacacc gcaaaattac agaaaattct tattataata
    54661 ataagggagt gcaattacct gcgcagttcg aagttcttgc ccaggtagac ttttcttaca
    54721 tgttcatctt cggcaagctg ggttgatgtg cctgccatca ggattcttcc ttcaaacagc
    54781 aggtaagccc ggtcggttat ggaaagggtt tcgtgaacat tgtgatcggt aataagaatc
    54841 ccgatattct tgtttttaag tttggacact atttcctgga tatcttccac tgcaatgggg
    54901 tccacgccgg caaaaggttc atctagcagt atgaactttg gtttaagcgc aagggctctt
    54961 gctatctcag tccggcgtct ttctcctccc gaaagctgaa ttcccttgct tctcctgatt
    55021 ttatgcagac cgaattcggt cagaagcgtc tcaagccttt cattctgttc gcttaaaggc
    55081 attccagaca tttcaagaac ggccctgata ttgtcttcga ccgaaaggtt cctgaaaacc
    55141 gaagcctcct gggccagata cccgataccg cgccttgctc ttttgtaaac gggaagggtt
    55201 gtaatgtcct catcatccag gaatattctc ccctcctttg gcctgatcaa acccacgatc
    55261 atataaaagg aagtggtctt gccagcaccg ttgggcccga gcaaaccaac aatctctccc
    55321 tgctcaacat ttatcgaaac atggtcgaca accgtgcggt tattgtattt ttttaccagg
    55381 ttttcagtat aaagtttcat gacaggaaaa tggaggagat cattttgccg gctcagaatc
    55441 tgcactcaaa tattctttgt gctccttttc tttctttttc atgacccttt tcgaaataag
    55501 tatccccact tcataaaggg caagaagggg tattgccaca agcatctgtg aaaacacatc
    55561 aggaggggtg ataattgcag caacgatgaa tatcactata attgcatgcc ttctgtattt
    55621 gatcatgaac gggggagtta ttacacctat cttggtcagg aagaatgcaa tgatgggcag
    55681 ctcaaaaacc agcccggtgg caaggcagat agaagtgatt gtgcctatgt aggatctgat
    55741 gttgatctgg ttaacgacat cggagctaac ctggtaattt gtcaggaagt ggatcgagag
    55801 aggagcaagg gcaaaatacc cgaataggac acccgtgaaa aaaagcaatg acgttgcaac
    55861 cacagcacct cgggtatatt tcttctcatt gctgtgcagg gcaggcttga aaaactgcca
    55921 gaactcccac attatgtagg gaaaagccat tatcaaaccc gatacaaggg caacaattat
    55981 gtgggttgtc agctgtccgg ccattttgat gttgatcaga ttgaccggtt tgctgtttat
    56041 gcataatgca tctacattga ccaggtgtcc aagcctgcac aataaacggt ttgtcagaaa
    56101 atcaggattt ttcggtgcca gaatgatctt gtcgaaaatc aggttcttca ggatgaacgc
    56161 cgttatcata aaaacgatta tggcaaggat tgaccggatc agatgccatc tcaactcttc
    56221 aagatgttcg aggaatgaca tctctccctc gttgtttctg ctgctcttgc cctttttggc
    56281 catcggctca aaagactaat tccttaaatt ttcagtgtgc aaagatgata aaacttatct
    56341 tgcaaaacaa gttcaattag gtttaacacg aaaatctcag atatggcctg cagatttttt
    56401 aaacaaaatt taatagtatt tgattaattt attatataac tttattaaac tgtaattgac
    56461 agcagattgt gagataagag aaatcaggtg aatgaaatgg taagtgatgc tgctatacat
    56521 gaatatttga aaaaatattt cggatttgat acctttaagg ggaaccagga atccataatc
    56581 aagaatgttc ttgagggtcg tgatacattt gtacttatgc ccaccggagg cgggaaatca
    56641 ttatgctacc agttgccggc agtaatgaaa cccggaactg caattgttat ctctcctctt
    56701 attgccctga tgaagaacca ggtggatgca atgcgcaact tcagcacaga tgatgatgtt
    56761 gctcattttc tcaattcgtc acttaccaaa tcggaaagtg ccagggtaaa acaggatgta
    56821 ctttcaggta aggccaaact tttatatgta gcgcccgaat cactgacaaa ggaggaaaac
    56881 atcgatttcc tgaaaaacgt cgatatatca ttttatgcta tcgacgaagc acattgcatt
    56941 tccgaatggg gtcatgattt ccggcctgaa tacagaagga taaggcccat tatcgactca
    57001 atcggcagat cgcccatcat cgcccttact gcaacggcaa ccccgaaggt gcagcacgat
    57061 atccagaaaa accttggaat acttgaggcg gatgtcttca aatcctcatt caacaggcct
    57121 aatctctatt atgaggtcaa gtcaaaacag gatgttaaca gggaaataat aaaatacata
    57181 aagaacaatc ccgggaaatc gggaataata tactgcctga gccgtaaaaa ggtggaagag
    57241 atggcagaga acctgaaagt gaatggcata aaggccctgc catatcatgc cggcatggat
    57301 tcggcaaccc gtacggcaaa ccaggataag ttcctgatgg aggaagccga cataatcgtt
    57361 gcaaccattg ctttcgggat gggaattgac aagcccgacg tgagatttgt gatacattat
    57421 gatatcccca aaagcctgga aggatactac caggagacag gaagagccgg acgcgatggc
    57481 ggtgagggta attgcattgc ttattacagc tattcggata tagtgaagct tgaaaaattc
    57541 atgcagggaa agccgatcgc tgagcaggag atcggtaaac aactgctgct tgaaactgta
    57601 tcatatgccg aatcctccgt gtgcagaagg aaactgctgc ttcattattt cggggaggag
    57661 tatccgaaag ataattgcga agcctgcgac aactgtctgc atccaaaggc tcagtttgag
    57721 ggaagcgaag ccgtggtgaa cgtgcttgaa acagtgcttg cggtgaagga aaaattcaag
    57781 gccgatcata ttgcacatat cctttcaggt aaaatcacct ctgcaatcaa gtcatacaag
    57841 caccataagc ttgaatattt cgggataggg gaggataagg atgagaaatt ctggaatatg
    57901 gtgatcaggc aggcccttat tgccaagttg ctcgcaaagg atattgaaaa ctacggactg
    57961 ctgaaggtca cacccaaagg aatggagttc tctgaaaagc caacctcatt catgctggcc
    58021 gaagatcatg attatgccga tccctccgat gaggataatg ctttcggtgc aagaactgcc
    58081 gccgttgacg aggaactctt cagcatactg aaggatctga ggaagaaaat ctcaaagcag
    58141 aaagaaattc cgccttttgt aatatttcag gatccctcgc ttgaggacat ggcaatacag
    58201 tatccgataa acctggatga acttcagaat atatcaggag tgggcgcagg caaggcacaa
    58261 aggtatggaa aggagttcgt tgagataatt aaaaaatatg ttgaggaaaa ggaaataatc
    58321 cgccccctcg acatggtggt aaaatcagtg gttaataagt caggaataaa ggtatatatt
    58381 atccagagca tcgacatgaa aagg
//
LOCUS       DAOOYG010000003        56352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_469_length_56352_cov_8.221468, whole genome shotgun sequence.
ACCESSION   DAOOYG010000003 DAOOYG010000000
VERSION     DAOOYG010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 56352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_469_length_56352_cov_8.221468"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK288_00540"
     CDS             complement(<1..350)
                     /locus_tag="PK288_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM17431.1"
                     /translation="MHNNHETTSEKELKKAVLIVAAFAAFLTPFLGSSVNLALPSIGK
                     DLGASAIELGWVISSFILSSAIFLIPFGRLADIVGRKKVFSAGLLLFTLSTTIIIFSR
                     SFAFLIVMRICQGIS"
     gene            complement(382..834)
                     /gene="dtd"
                     /locus_tag="PK288_00545"
     CDS             complement(382..834)
                     /gene="dtd"
                     /locus_tag="PK288_00545"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPM17432.1"
                     /translation="MRAVIQRVSRASVRIGHTEKSSIGHGLLVLLGIEENDSDTDTDW
                     LCKKIVQLRIFNDNEGIMNLSVRDVNGSIMVISQFTLHAKTKKGNRPSYIKAAPPEKA
                     IPLYNSFTEKISELLGKEIATGEFGAMMEVELVNDGPVTIIIDTREKE"
     gene            1184..1747
                     /locus_tag="PK288_00550"
     CDS             1184..1747
                     /locus_tag="PK288_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPM17433.1"
                     /translation="MDLITIVAVAAGLSFDSFAVSLSLGVVRNRILLKEAVSVALVMA
                     LFQASLLVAGYFLGSLLSDVLTAADHWVALLLLTFLGARMIIEGLRSEKEKKQRDYHN
                     PFTIITIAVGTSIDAFAVGISFALLNIRIWCSALVIGIVTFIASMLAIGIGKSAGAHF
                     GRNAEILGGIILILIGIKIFVEHMFSI"
     gene            complement(1903..2688)
                     /locus_tag="PK288_00555"
     CDS             complement(1903..2688)
                     /locus_tag="PK288_00555"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPM17434.1"
                     /translation="MIYKTDYHVHSWFSDGRAAPEDYIAPALEAGLKELGFSEHLTLF
                     EKNLDWSMNARKAYRYLEHMKVFRENNHDITVRTGLEVDYIEGREKEINSFLKKADID
                     YVIGSVHFLGESTVDMGPEFYAGKDIDRLYVVYFEQVASAAASGLFDIIAHPDLIRIY
                     GFTPSFDLEPLYRKLAASFKKHDVAFEINTNGRNRPIADFYPDRRYLKFFREEGVPVC
                     VNSDAHMPSRVAQYFDEAYELLRNTGYTEMAVFKDRERFMIPF"
     gene            2807..3502
                     /locus_tag="PK288_00560"
     CDS             2807..3502
                     /locus_tag="PK288_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPM17435.1"
                     /translation="MAKKDRSCECEKCDFREVVFASLDEQNIQALCEIKEERHFRKGE
                     IINYEGERITDFKYLKTGLVKLFRRSSNGEEQVITITRPFEFVSNMSIFSEERYKYSV
                     SALEESVVCCVKLDFIKELFIKNGQFAINLMSKISVINDKIINQTLDIRQKNLAGRVA
                     YVLLYFTKEIYKSRVFDIPVSRKEIADYIGMSTANVIRTMSDFKKDNIIRVFGKTIEI
                     ADIDKLEIISKRG"
     gene            3565..4797
                     /locus_tag="PK288_00565"
     CDS             3565..4797
                     /locus_tag="PK288_00565"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPM17436.1"
                     /translation="MVSSKKQKNMANIKSTKGLIAILTGGGDVPGLNPAIRAVTIRAN
                     REGYKVIGLRRGWAGITELIRDHKADNSNCYQVLTDDIVNKAGRTGGTFLHTSRTRPS
                     HMSKAAVPDHLKDTYNADMNDLTPEVIKNLEWLGIDYLIPIGGDDTLSYGVHLYKQGV
                     KVVAIPKTMDNDVPGTDYCIGFSTCVTRTIQMTNSLRTSAGSHERFLVLEVFGRYAGF
                     TAMLPTMAGAANRCVIPEYEFDIDLLTKLLVEDRNHNPSRYSVVLVSEGAMFKGGEMV
                     FSGTEKDAYGHAKLGGIGDLVSEKLKEYSPKYNNGKTINIINQKLGYMVRGGDPDALD
                     SIVPMAYGNLALDLILKGMHGRIVVLKNGRYDNVPIDVVTSSKKIVKVSEHYNTERLR
                     PFYHSFEMKPFLLMASDS"
     gene            5030..5956
                     /gene="rsgA"
                     /locus_tag="PK288_00570"
     CDS             5030..5956
                     /gene="rsgA"
                     /locus_tag="PK288_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPM17437.1"
                     /translation="MEKGKIIKSVGSRYKVLLDSGRTIDCLLRGKLRVNEIRSTNPIA
                     VGDNVEIELSGTSGTGIITALLDRKNYILRKASNLSKSSQIIAANIDQVLLMLTIALP
                     QTPLEFIDRFLITAEAYRIPARLVFNKTDLYGKEEMERLDQLVSIYTGIGYGCHRLSL
                     RDGSGFDELKTVMKDKINLISGNSGVGKSTLLNLIDPAFNIRTEEISDFHKQGKHITT
                     FPQMYRLPFGGFAIDTPGLRGFGIADMEKNEIYHFFPEIFRKSKDCRFYNCLHLDEPE
                     CAVRKAVEKEEINYLRYRSYLNIMGDDNAKYR"
     gene            5996..8824
                     /locus_tag="PK288_00575"
     CDS             5996..8824
                     /locus_tag="PK288_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM17438.1"
                     /translation="MKMNKILWAVSAALLLIVLASSLVFSGLYNKQISYIKNLLDREV
                     RIAGSSVDQINNEFSSDLNQILFSVDHSLFFTDPVQQMKIEDKIKFFFTKYESVITGI
                     KFFDNNRNGYTLKKDETGNNWLIHNFVLHAQEEIHSRDELIKGSRGYEYYMPVLKNGT
                     LIGNFIVSIDFNKYFNDLFSGFNAREYQWQWVISDSGSVIYDNSGQSGKYSRIDRLTR
                     LVAEGASENIVHSFAIEGNHKKIITSFYSTQLFQKEIGLMFSAPADKFKNYILKVTLA
                     MALLAIALILGIFWLFVQHIRSLEEKSAKLKSSEEMLFRMIDSIPAGVIIYNGNREIM
                     MANKEAANQFSYQSESEMKGRIYPGNKSTDESAYFLKNLAGTFSHDQFVIIKKEIGEL
                     ILYRNTIPVSFQGEDASLDVLFDVTMMESARKQAATANTAKSEFLARMSYELRTPLNG
                     IIGMADILIKSRLSAENRAIVNLLRQSAEVLLNIVNDILDFSKIESGRMILDEIPFSL
                     REEILYSYDLARTNIDENQIKLTCSVDEEVPDKLIGDPYRLRQILSSLLSHSVDNTAR
                     GSINLKCSLAEKHNGTMNLRFELTDTGKSFDRATIKKIFGEQINIESKVHGDDDGSGF
                     GTILARQLIELMGGKLNAESPSGLDGDKGLKISFTISLNLNETVIKDLQFDDITSFGQ
                     IRTLVISGGHLKDEEILNSLYKLGLTVSVTSYQKSTINQLMANLSFPEKRYHLLVIAD
                     NGEFSGFAVAQDLWDNNLSARFIIMIISSNDARGNLLKCIRLGVDNYIVKPFEIGELY
                     DKIRMKFSGTDRQADRQEAEAGSRNIRILVVEDNKLNQKVLGTMLKSLGYSFDFADDG
                     FAGLIQAKTRKYDVIFMDLLMPRMDGFEAARQILEYDNSQLIVAFTADNLPDSRRKAE
                     LAGIKEFISKPVRIDELREFFRKHFFKN"
     gene            8893..9939
                     /gene="mnmH"
                     /locus_tag="PK288_00580"
     CDS             8893..9939
                     /gene="mnmH"
                     /locus_tag="PK288_00580"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HPM17439.1"
                     /translation="MTFQLGQKLDISLFLERVEHIPVVDVRSPAEFSAGHIPGAFNIP
                     LFDDEERKAVGIRYRKSGRFGAINEGLKLAGPSFHLKLEKARKLSADNKLLVHCWRGG
                     MRSEAMAWLFSLAGIETGILEGGYKAYRQYILNSFSENRRMIILGGFTGSGKTEILRN
                     LRNAGEQVIDLEGLANHKGSAFGSLGQPAQPTSEHFANLLYEQWRRNDIEKPLWVEDE
                     SKNIGTVFMPDEFFLNMQKNPVIVLLIDFRIRLHRLVREYAGYSVADLILAIRKISRR
                     LGGENAMDAISSVECGDFTKAAEIILRYYDKAYLYGLKKKQAEKIVYVESDSDDAAIN
                     AERVLEASGQLCRN"
     gene            complement(9957..11201)
                     /locus_tag="PK288_00585"
     CDS             complement(9957..11201)
                     /locus_tag="PK288_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPM17440.1"
                     /translation="MDRKEIEIMAPAGSYESLIAAIQGGADSVYFGVGQLNMRAASSV
                     NFSLSDLKSIAEICRDNKIRSYLTVNVVVYDNEIEEMRRIVGSAAESGVSAIIASDLS
                     VIDYAFRSGMEVHLSTQLNIANTEALRFYSQWADVVVLARELDLGQVREIYDNIRKQD
                     IRGPGGELVKIEMFVHGALCMAVSGKCYLSLHENNKSANRGTCYQTCRKSYIVTEKES
                     GNQLEIDNQYIMSPKDLCTIGFTDRMIEAGVRVFKIEGRARSPEYVRFVSSCYNEAVN
                     SVLDGTYGSEKIEIWMERLSAVFNRGFWDGYYLGQKSGEWNTRYGSNATMKKVYLGKV
                     VNYFTRLKVAEIKLENGDLEKGDLLLITGPTTGVIEYTADEIRVDLMATEKALRGESC
                     SVRIPDYLRRSDKVYKWVKAGR"
     gene            complement(11235..12359)
                     /locus_tag="PK288_00590"
     CDS             complement(11235..12359)
                     /locus_tag="PK288_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM17441.1"
                     /translation="MFLFLFAILLGVGSLIYTQSLVKNLKSEERKKVQMWAEATRLIN
                     SADSTQNLEFLLSIIENNNTVPVILTDGHDNIIAVRNIDLGKEGDRGSLGVSLEKMKS
                     RNNPIIIDLGGGFINRIYYKDSIILTQLIYYPYVQLGLIIMFILVSYQALSSSRKAEQ
                     NRVWVGMSKETAHQLGTPTTSLSGWIEILQNNYPDIQVTRELALDVKRLEKVTERFSK
                     IGSKPELSEENIISLVQNSVDYLKSRSSNKVLFVLTFSSDDEILAPVNPALFEWVIEN
                     VCKNAIDAIEGSGEVTVRVTEDEKYAVIDISDTGKGMSKTAYKKIFNPGYTTKKRGWG
                     LGLSLSKRIIDEYHNGKIFVKSSEIGKGSCIRIMMRKSGA"
     gene            complement(12421..15042)
                     /locus_tag="PK288_00595"
     CDS             complement(12421..15042)
                     /locus_tag="PK288_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPM17442.1"
                     /translation="MKFPKKILKLAGILVVSAVIVLFALSVALQDKVAEVIIRALNKN
                     LATKIEIGSYHLSLLRKFPKASFELKNIRVYSSPGFEKSGFSDMNTDTLLAAASASID
                     FKMIDLFRGVYSFKAVNVRSGNLNLFTDSAGRYNYSFTKRETQGDAEREVRLNLDRIN
                     VSDVRVLYSDLRADLVIRGLLKNGRIRSRIRGANIDFDGSSDVLFTHFSLKRTVFNRS
                     FNARMDVGLNRNEKGIFFRKSTISFDKQDIIMTGLIIPDDNYLDLSISGNHIDISKAF
                     HYLSGNYKTIVSEYRPYGIFDLDCRIKGKSTRFEDPHYDIAVSLEAGGLHRVRSGLKM
                     NDVRFTASMTNGARNRPETSTLGISDFTAGLGSSVFSGSFSASDFSRPRASLKFRGTL
                     IPSELREFAGMEALGPCSGSVDLDISLSGVIEKKDHYSLSDLAELNSRSEIIFRSFGT
                     EVPGRNFNLRNTNGKLVIAGSTSAEKLEFNLNDQEFSLSGNLDNLPKWLAGKPVNLTG
                     HAYVYSPCLKPEYFFSSPATRDETVKARAPLALPRDVMIDLTVKLDSLKYKKFDAGQL
                     HGEISLKPGMLNFRSLRFKSQDGTVTGNGIVVQNRNKSFTGRGSFSVSNVDVKKSFIS
                     FNNFGQNFLKAENIAGSLSGTITLVLPADSMLNPAMKSLVAEGKYSLSDGALINFDPV
                     KALSKFISLSELENIKFDKLDNDFFIRNNYFYVPVMQIRSSAVDLAVNGRHSFDNAYE
                     YHVEMLLSEILSRKAKKNRKTSSEFDEVEDDGLGRTRVFLKLMGKGEDVDVTYDMKAA
                     GSKIKNDLRNERQMLKTILNEEYGWYPSETEQVKKETASKPRFRISWEGTETTGTEQK
                     TSSEKKDGGIRKFFRKK"
     gene            complement(15115..15822)
                     /gene="pepE"
                     /locus_tag="PK288_00600"
     CDS             complement(15115..15822)
                     /gene="pepE"
                     /locus_tag="PK288_00600"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HPM17443.1"
                     /translation="MRLLLISNSTNAGEPYLDYPKHRIRDFLGRAPVRALFVPYAAVT
                     FSYDDYEKKVQERFREIGHDVISIHHWSDPVRALADAPAIVVGGGNTWMLLKLLRQNG
                     LILPVRKKVLSGTPYIGWSAGSNVACPTIQTTNDMPITRPSSFRSFNLVPFQINPHYL
                     DASPEGHAGETREQRIAEFIEANPDVWVVGLREGTMLHVVDNRINLTGPGKARIFRKD
                     TIPAELGEPDDLSFLLR"
     gene            complement(15891..16664)
                     /locus_tag="PK288_00605"
     CDS             complement(15891..16664)
                     /locus_tag="PK288_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HPM17444.1"
                     /translation="MSDFILTPRELRRYSRQIMLPETGKEGQLKLKMAKVLVIGAGGL
                     GCPVLQYLAAAGVGKIALAEFDTVDEGNLQRQVLYGSDDLGKLKSIIAARRIEQLNCM
                     INTVVINLKIDSRNSLSILRNYDIIVDATDNFRTRYIISDSCVILDKPMVHGAIYKHE
                     GQVAVFNYMGGPTYRCFNPFPPAENGVFSPSETGLLGVLPGITGTIMANEVIKIITGY
                     GEVLSGKILIFSLANYRSGIFTIKANPENRKLTRLYDNY"
     gene            complement(16766..17734)
                     /locus_tag="PK288_00610"
     CDS             complement(16766..17734)
                     /locus_tag="PK288_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17445.1"
                     /translation="MRTFIFIFPALMAFLQAPGIHADDTAKAGRQHSVIEEADGSMNI
                     AVGKDFFSFDENDSSVTVRLGSRGIKILESLEGKKVEFEKYDTESECDEESGTHFRDD
                     DDRSWLLKARRFRGHLAGFEAGLNNYIHEGSMVLPGPIDYMSPETSNSWSFNLNFSQL
                     NIGFGRYSGLVTGMGLNWNNYRFRNHTTIEVGEEGTVTGKLPASSVPVRRSKFSTLYL
                     NVPLLFEIQIPAGYNGRLNLAAGVTGGLKLNAWTKIVYKNGEKSRVNGDYNLNLLRGG
                     FTGRVGYRNFMIYGTYYPTPWFQKNRGPEGMNLEPFEIGLAFTFND"
     gene            complement(17795..18895)
                     /locus_tag="PK288_00615"
     CDS             complement(17795..18895)
                     /locus_tag="PK288_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17446.1"
                     /translation="MKTAPERSDFEILLADWLAGNLDPRQEEVLKTFLEQNPGLAATL
                     PELKQVNLRPPDISYAKKDVLKKSPGSISEEQFVVMCIAGLENDLSAGQLEELDNIIS
                     NDDNRRKTFELIKRLKVRPQPAVFRRKSSVIKLTAGQRIFRYSLAGLGMAAMVALLIT
                     VRLLVSPSAEVIKNHTTSPLTAESGTSAGHNAVNEEKSLITGSRSNQQAETGSKTDEI
                     KIADKKPPADQTVQVTTDESSRAIPASLPAPPAPLAAGITLIPVAPPDPNSQILRKFN
                     PSNLVPEYPVHRSNVDRLLARLVHGFIMNDTLPGDRPVKSFDLAIVSITGINRLLGWD
                     MTLDRITDGKGELRSYYFSSGLLKFNAPAKKP"
     gene            complement(18892..19377)
                     /locus_tag="PK288_00620"
     CDS             complement(18892..19377)
                     /locus_tag="PK288_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM17447.1"
                     /translation="MTVKEYNRSVEEYSDYVYRFILASIRDEEKASDIVQDTYEKLWR
                     NVMEISYPVVKSWLFTTAYHIMIDIIRKESRMSPMEDATGNDLIDESQYSDLNEILHM
                     ALERLPHIQKSLILLRDYEGYSYREIGEITGLTEAQVKINIYRGRMALKNFIGKVETV
                     L"
     gene            complement(19491..21104)
                     /locus_tag="PK288_00625"
     CDS             complement(19491..21104)
                     /locus_tag="PK288_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HPM17448.1"
                     /translation="MASLRLRLGWIPTTSKIEQAEKALIADYEKLQALTRSDLLAQFT
                     GLREKVTSSEFIQKKKDIESLRYRDSEEYNKEKEFLSLQKAKDIVMYFKTSAGNMLKR
                     FKELDGSQKIRDFEALETFIRSNGFKLKKKMKPITFRDTDEYRKFLEYKSLKSDPEIR
                     AYYKASAKGEAVKSKAVEKFEELGAYIKTPEFLAKKNMKPITFKDTEEYKKLLEYKRL
                     KDSIEIREYYNFKSSAEYANFLNTDGSSRLDRYNKLREYLATNEFRDRKNYLLDKKRF
                     EKTDMFREISEFEKLQKNEDIVWYFKMKDSDRFDVLKKKTLTFADEFEGDKLDTDKWL
                     TNYYWGDKLLKDRYSVEGDLQAYTERDNFEIRNSLLRIHTKPQRITGKVWSPDLGFRT
                     REFSYTSGIINSGISFRQKYGIFTAKIKLGNPDARNTFWMLAGEMTPHIDICRTSGGK
                     VWADFSPSPQIKLKTLAGSRYARDFYIFSLEWTSSMLVWKINGEVLFRQTSNIPQEPM
                     YVLLSGGTEKPLSGMSTMEIDWVRIYQFS"
     gene            complement(21281..21898)
                     /locus_tag="PK288_00630"
     CDS             complement(21281..21898)
                     /locus_tag="PK288_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17449.1"
                     /translation="MKRAVYSLIAAAAIILANGCSKEYELNKSIFIPDPDFPELPAYT
                     EWGYNTFGAYYERGLFLYSENAIPAKIIRAGGITSFVLKGQKDGYGYHSWDNRKEMSV
                     TFLLPSVMPDSYADLTILDKKIIDLVNTPGAVKLFLDGDTIKPDILQGELEFRRVQLL
                     YVDSKQTEVILSGVFSFQALIKDEPVSVTQGRFDVGIGEDNFFSY"
     gene            complement(21910..22677)
                     /locus_tag="PK288_00635"
     CDS             complement(21910..22677)
                     /locus_tag="PK288_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17450.1"
                     /translation="MKLKILLSVFLSTIVAVSGLSQDTKTRCLSVDAGIDFISGGPPE
                     KDYIRADRGQFFSDFPTTSLRGLFYRNYVSIKGEKTVLNNKVGLVSGIRYTRTESSIG
                     KESYWSNSSDFFYLLLRQEETRTDYLKVNEITQLAGYLAIPLEIRIYPYRPRAFNVYY
                     KIGGDVNYRLHSRARTVFFNRDMKVFEDEIENIIEKPWVFYSSFHLGVGFRIGMVDKP
                     GVNVEIHIPVTMIRGGKQDFVTPEAGTGIQLNARIPF"
     gene            22901..25054
                     /locus_tag="PK288_00640"
     CDS             22901..25054
                     /locus_tag="PK288_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPM17451.1"
                     /translation="MTDFFDHLMDEFVLPLQNPVLVFSLILFIILISPILLRRLNIPG
                     IIGLIISGVIIGPHGLNILAKNSAIDLFSTIGLLYIMFIAGLELDLNEFKANRNKSLL
                     FGLFTFFIPLAIGYPVCFYLLGYDFNASFLTASMFATHTLVAYPIVSKLGISKNQAVA
                     ITVGGTILTDTAVLIILAVIMGNSQGNLSQEFWIRLGISLAIFTAIMFLIIPRIAKWF
                     FRKLESEKHSHYIFVLSVVFFSAFLAEVAGVEPIIGAFMAGLALNKLIPHSSALMNRI
                     EFIGNSLFIPFFLISVGMLVDVRVILKGPMALIIAGTLSVVALTGKWLAASFTQLVFG
                     YSKNQRQLIFGLSGAHAAATLAVILVGFRAGILDENILNGTIILILITCIVASFATEK
                     AARKMVVEAEEDDAVLATANGVHNEHILLPIANLENIEKLLEFSIYIKDKKSINPVSV
                     LTVVSNNDEAEINILKARKKLEEFVKQASATETKVNVITTIDHNPASGIARIAREIMA
                     DIIVLGWPRRTGLIGKLIGQKVDSILSNTDKSTFICYMKKPLILHKRIIIAVPPFAEH
                     ERGFEFWLLKIAKLAQELSVPIQFYCNESTEKSMRRVLKAAKQSASVSFTRFIDWEDF
                     LILARYIREDDLFVLVSARKGAASYISVLENLPMKLEKHFSLNSRLVIYPQQFNQTYA
                     TDGYEDITADPLNKGIETIQKIGKGISSIFKKGDI"
     gene            complement(25064..26278)
                     /locus_tag="PK288_00645"
     CDS             complement(25064..26278)
                     /locus_tag="PK288_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HPM17452.1"
                     /translation="MKNSFTRTVIFATILILSGCRPGVQTDRQDDRAEKPGYYVAAYI
                     WPSCHDDPLGREMLWPEGTGEWEVIKKGTPRFEGHYQPRQPFWGYEADNDPKVVEKWI
                     EAATSHGVNVFVYDWYWYNEGPFLESALNDGFLGARNNEKMQFYVMWANHDVKYNYWN
                     VHRYKDNTDILWNARVDWKNYRIIVDRLINQYFRRPNYFKINGEPVFSIFSVDKLLES
                     FGNSTEEARRALDYFRDEVKKAGFPGLHIQWNQGGGSIMSEAAAAKFAGNVKAMGFNS
                     VAMYNMGGLDEDYIVYGTNSIRIREQMDSLLDIPVFPCVSIGWDDTPRFPAKGMHDVV
                     HYHNTPRSFAALLARAKRYADSHPGQTRLITINAWNEWVEGSYLLPDMLNGFGYLEAV
                     RDVFIDGKYDRF"
     gene            26905..27483
                     /locus_tag="PK288_00650"
     CDS             26905..27483
                     /locus_tag="PK288_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17453.1"
                     /translation="MMKRILLIFSAVIMTTSAAGQDLEISVAPTVNFPVYKALEGGAG
                     HNPGTGFSITFGYLAAQKQFIAWTIGLGYHFVRIKTDPMFNGVYDPLPYTESCSLISF
                     SIGAVSNLRNNFFIAFGPAMDLQLNQKGQRTIERQTGLGFSLSGGKNISLDNNLGLRI
                     RPEIRSHNLIPFQGWDYPLRLLVAGLNIGLIF"
     gene            27787..28389
                     /locus_tag="PK288_00655"
     CDS             27787..28389
                     /locus_tag="PK288_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17454.1"
                     /translation="MKKQNTTSLIKIFGTVLIITIFFSCSTVNRIYTDNDLTRSSKRF
                     ELKYLVRDRVRRSPLHFFRQTIIKEIGRDNEVNYTAYDVLTLSGTSFTVDDKVFLIID
                     NVPFSMKIDRMEFENERSLSEDTQTFLNSESVPITVMTGYSEDNSKIARFSYKIPPEA
                     IERIRNSNQLLIRYYSGPSMMTVKPGKKSFRKLKQLIDSF"
     gene            28640..28993
                     /locus_tag="PK288_00660"
     CDS             28640..28993
                     /locus_tag="PK288_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17455.1"
                     /translation="MKAVNTGSRRIRVSVFGILGAGIPRTVSNAGNNPAAGELTGYSG
                     NYHPGISAGAGCRFPFGLAESGLSGKAATVKNNERDNNLIEYQSNTYCSFSRTIEGYF
                     GGGPGVFREQYFLKG"
     gene            29062..31269
                     /gene="katG"
                     /locus_tag="PK288_00665"
     CDS             29062..31269
                     /gene="katG"
                     /locus_tag="PK288_00665"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HPM17456.1"
                     /translation="MEKQPKKASKSPVTGAVSNHVAGMAGTKNRDWWPNQLKLNILRQ
                     NSSKSNPMGEGFNYAEEFKSLDLKAVKKDLHRIMTDSQDWWPADFGHYGPLFIRMAWH
                     SAGTYRIGDGRGGAGSGQQRFAPLNSWPDNVNLDKARRLLWPVKQKYGRKISWADLMI
                     LAGNVALESMGFRTYGFAGGREDVWEPEEDVYWGSETKWLDNDKRNLDPEETANPLAA
                     IQMGLIYVNPEGPGGNPDPLAAAKDIRNTFARMAMNDKETVALIAGGHTFGKCHGAGD
                     ASLVGPEPEGAGIEEQGLGWKSSYGKGKGEDTISSGLEVTWTPTPTMWSYYFFDILYK
                     YDWELTKSPAGAYQWVAKTTNKSIPDAHDPSKRHLPSMLTTDLALRFDPVYGKISKRF
                     HENPLEFAEAFARAWFKLTHRDMGPRSRYLGPEVPAEELIWQDPIPVVNHPLIDNEDI
                     AALKSEVLKSGLTVSRLVSTAWASASTFRGSDKRGGANGARIRLAPQKDWEVNDPPSL
                     KKTLGILEEIRDRFNDAQTGGKKVSLADLIVLAGCAGVEKAAEDAGFKINVPFRPGRM
                     DASPEQTDAESFNVLEPLADGFRNYLKARYTVRTEELLIDKAQLLTLTAPEMTVLVGG
                     MRVLNTNYDNSGHGVFTNRPEVLTNDFFVNLLDMNTVWTADDDGRETFKGHDRKTGKL
                     KWTATRADLVFGSSSELRAIAEVYGSADAKEKFVNDFVAAWSKVMDLDRFDLG"
     gene            31612..33069
                     /locus_tag="PK288_00670"
     CDS             31612..33069
                     /locus_tag="PK288_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HPM17457.1"
                     /translation="MDKISRRKFLEKSLLINGSVVLGSAWICPFLESFTLPQAILYHV
                     QIQDDNPAIAWSQDKCKGCRDCLTACAEKQLIQGSYKSVRGHHVCIHCGACIQVCPYG
                     ALTEKYNYREVFSALEDPSKIVIASIAPSVPAGIGEFFGLPSGSYLDGNIAGACKTLG
                     FDYVLNNNFSADLTIMEEAHELQRRMEGNSALPQFTSCCPAWVKYVEMYYPSLVKHLS
                     TTRSPFSMQGALVKSYFARKKKIDPARIVHVAIAPCTAKKYEIGREELTTDGLRSTDI
                     EITTNELAMMLREKNIHIAGQKGAFDSSMGKASGSGIIFGNSGGVMRAALRTAHYNLT
                     GKNPAGDFLELKELQGLTGLKEATVKIGDRSLNVAVVYEMRNAKPILEQVMAGTCKYD
                     FVEVMACQGGCIGGAGQPAKTISDLENRMKALDSADAGAAFRFSHENPEIRTIYRKFL
                     GKPGGKDAERLLHTYYTDKSELLVPAKSRKPAKVS"
     gene            complement(33551..33623)
                     /locus_tag="PK288_00675"
     tRNA            complement(33551..33623)
                     /locus_tag="PK288_00675"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33588..33590),aa:Gln,seq:ttg)
     gene            complement(33745..34173)
                     /locus_tag="PK288_00680"
     CDS             complement(33745..34173)
                     /locus_tag="PK288_00680"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPM17458.1"
                     /translation="MMKKMNKYCVEATKRTPAICLEHGRIIFEGRSITENPGAIYRPV
                     HEWISRYSRICREETQIEFGFEYINTSSTKWIYNIIKELAGIENVGKYARILWYYEQG
                     DDDMCELGLILRSLTECPFFIVETAGLKKKFADTQFLNAL"
     gene            complement(34554..34790)
                     /locus_tag="PK288_00685"
     CDS             complement(34554..34790)
                     /locus_tag="PK288_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17459.1"
                     /translation="MKRNLYKEDSLKRTSRFSVMLNSREARALNIYCARYRIKNRSEF
                     MRETIMKAILKRFEDEHPSLWEKPEPTLFSRMTR"
     gene            complement(35094..36065)
                     /locus_tag="PK288_00690"
     CDS             complement(35094..36065)
                     /locus_tag="PK288_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPM17460.1"
                     /translation="MLHYKELGLVNSRELFKAAMKGGYAIPAFNFNNLEQLQAIISAC
                     VETKSPVILQVSKGARKYANQTLLQYLAKGAVEYAKELGYQIPIVLHLDHGDSFETCK
                     SCIETGFSSVMIDGSHFPYEENVRLTKQVVDLAHQYDVTVEGELGVLAGIEDEVSAEH
                     SSYTRPEEVEDFVKKTGVDSLAISIGTSHGAFKFKVKPGELPPPLRFDILEECEKRIP
                     GFPIVLHGASSVPQDIVEEINKYGGRMENAAGVSEEQLRRAARSAVCKINIDSDGRLA
                     MTAAIRKVFVEKPGEFDPRNYLGPAREKLKELYKHKIVNVLGSAGKA"
     gene            36367..37707
                     /locus_tag="PK288_00695"
     CDS             36367..37707
                     /locus_tag="PK288_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM17461.1"
                     /translation="MKTFPLIIIIFLLISPGKTQAQQEPWSLEKCISYAVDHNIQIKQ
                     QVLQTRVQQNNYNQSKLNLLPVINGSASHDYSFGRALDQNSYEFYNQTVLSDYFYVGG
                     RTNIFSGLQNYNSIQKSRYELLAGQQDLHKISDNVSLTVALAYLQILLNKELVTVNEN
                     QLNITLQQIEKTRKLVDAGSVARGNLLQIEAQAAQEEVNLITVRNLLELSYLDLTQLL
                     ELESPAGFEIEVPTIDIGAISLIPGSVEEIFSLAAQSRPEVKSSELRLAAARIDLKIA
                     RGSRIPSVSLSHSFGTRYSYIYDLPGLQSFGDQLRNNKSYGLGVAVNIPILNGWQVNR
                     NISNTKLAVENSLYSLEGTKKQLYKDIQQAYADASAALKKYNASQKAVNSMEESFRYT
                     EQKFNIGMVTPVDYNASKTQLLKAQSDLAQSKYEFVFKTKVLDFYRGIPLNLDK"
     gene            37731..38993
                     /locus_tag="PK288_00700"
     CDS             37731..38993
                     /locus_tag="PK288_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM17462.1"
                     /translation="MRSNNKILKILLPAVVVLLIFAVIGKKQGWFGQAARVKVAVINA
                     ERRVIVETITANGKIQPEKEVKITPDVSGEIVELTVREGDQVVKGQLLLRIKPDVYIS
                     QKDRSLAAISSARARLAQAEAQFIQAELTFKRTEQLYSEQTISKSEYEQAEASYRVAK
                     AEVDAARFSVVSAEASVKEADENLIKTSIYSPMTGTVSMLLVELGERVAGTGLMAGTE
                     MLRVADLSRMEARVEVNENDIPGVKMGDTASIEVDAYPEYKFRGVVTQIANSAKTTGV
                     SADQVTNFDVKILVLPESYSNIEGSSDTHPLRPGMSTTVDIQTRIRSGVIAVPIESVT
                     TRTDTTKLISSTSDKDMRTLVFITDGIRALARDVKTGIQDNSYIEIISGVSEGERVIS
                     APFSAISKKLSDSSFVEIVKKEDLFSIK"
     gene            39019..39738
                     /gene="tsaB"
                     /locus_tag="PK288_00705"
     CDS             39019..39738
                     /gene="tsaB"
                     /locus_tag="PK288_00705"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPM17463.1"
                     /translation="MIICLETSGTVCSVALCDRQGVVALRESAESKSHASMLTLFIDE
                     LLTDARLSVQELESVAVSKGPGSYTGLRIGVSVAKGLAYAASIPLIAVETTLSMVYNI
                     SDNIRQKYGVDNETLLAPMIDARRMEVYYAVYRSDGMKIKETTAEVVSEKTFNDIPES
                     VKILAFGDGAAKCRQVLKRKNLEFAGEFRISAAGMYKPAYDSFAAGRFEDVAYFEPFY
                     LKDFIATTPREGFLPGKQIAG"
     gene            39763..40542
                     /locus_tag="PK288_00710"
     CDS             39763..40542
                     /locus_tag="PK288_00710"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPM17464.1"
                     /translation="MKQLKVIIFCFLSSLCATGQNLAYMPGEKVDYTIHYGVLQGGVA
                     SLEIRSDTFGGKQVYHSVFLGKTTGLADVIFRVKDIYESYIDPVTQLPVFSIRNIQEG
                     RYRRYNEVIFDHHSRADSAILTSNLTGVHITEQGIHDIISCFYYFRSHHLPRYQSLKK
                     GDMITIMTWFTDELYPIRLRYLGTEEIRTKLGRIKCFKFNPVTEKGRLFKTEDDVSFW
                     FTADKNFLPVKVRFDIFVGSFIAEIAACEGLKYPLEIRKKE"
     gene            40569..41135
                     /gene="efp"
                     /locus_tag="PK288_00715"
     CDS             40569..41135
                     /gene="efp"
                     /locus_tag="PK288_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPM17465.1"
                     /translation="MASTADFRNGLVIEFNDDLYTIVQFQHVKPGKGPAFVRTKLKSI
                     RTGRVIDNTFSAGTKVNLARVERRPYQYLYQDDMGYYFMHMETFEQIHVPEELIGNHE
                     FLKEGQNVEVVVHSDTETVLSVELPQFVVMEVTYTEPGLRGDTATNTLKPAKIETGST
                     VMVPLFINTGDKIRIDTTTRSYIERAKA"
     gene            41292..42524
                     /locus_tag="PK288_00720"
     CDS             41292..42524
                     /locus_tag="PK288_00720"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPM17466.1"
                     /translation="MKNRHASSDRLKMSRFKLDALLDITLSINANLPAGDLLAKYESI
                     LRNNLQIGKIMIFKYSETWECILNGGFPENYRTGINVEKDLLKYGKTVFRDEIQGMEG
                     VEIIIPVYNNNVPLAFILIGDIDEEGRGVSPVIKHLNFVQTISSIILVAIENIRLFSE
                     SLRQEALKKELELAARMQQKLIPDNNLMPADGKLVVTGFYYPHYEVGGDYYDCIQLSD
                     TITGFCIADVSGKGISAAILMSNFQAALRALFTSDIGLVILTEKLNKLTVANTLGDMF
                     ITAFLARYDSSSGILEYVNAGHNPPVLYNTADGGITYLNSQCVAIGMFDEIPYIREDK
                     IIVRDYSKLVCYTDGLSDLKGDDGKEILTNEIVKNISNMEPVRDNIMDMLDQLGIPVG
                     NPGLFDDVSIMAADLFAR"
     gene            complement(42539..43831)
                     /gene="tyrS"
                     /locus_tag="PK288_00725"
     CDS             complement(42539..43831)
                     /gene="tyrS"
                     /locus_tag="PK288_00725"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPM17467.1"
                     /translation="MNFVEELKWRGMLHDIMPGTEELLNREITTGYIGFDPTADSLHI
                     GHLVQVMLLMHFQRAGHVPVALVGGATGMIGDPSGRSEERNLLDETTLRKNETGIKNQ
                     LSRFLDFNPERKNRAIMVNNYDWMKEYSFLGFIRDIGKHITVNYMMAKESVKKRLGSE
                     TDEGLSFTEFSYQLVQGTDFLHLYKTLGCKLQMGGSDQWGNIVTGTELIRRKTGGDAF
                     ALTCPLITKSDGSKFGKTESGNVWLDPDKTSPYHFYQFWLNVSDEDAARYIRIFTMLG
                     RNEIEDLTERHKAAPHERILQKKLAEEITVMVHSREEYESVAGASQILFGKGTTESLA
                     RMSESTFLSVFEGVPVYEISRGLISSGVSITSLCTEHTRVFESKGELRRMVQGGGLSI
                     NKVRILDPEEKITENSLINHKYLLVQKGKKNYYILRVI"
     gene            complement(43876..44412)
                     /locus_tag="PK288_00730"
     CDS             complement(43876..44412)
                     /locus_tag="PK288_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPM17468.1"
                     /translation="MNFIDLIIVVLLLLGAFRGFTDGFIKELASLAALILGIWGAIRF
                     SSFTAGKLYEYFDMTGRYTGIIAFIVTLVLIIILVHFVGVIVDRFVGSIELGFLNRLA
                     GIAFGLFKTALILSVFFVILNAIDARRPFLPKEKLQQSKFYGPVSDFAPALFPVIGEG
                     DFYKSFDRFKKKPEGIPI"
     gene            44536..46029
                     /locus_tag="PK288_00735"
     CDS             44536..46029
                     /locus_tag="PK288_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM17469.1"
                     /translation="MNDPVNSRRDFIRKSAALAAGFTIIPRHAVSGLGYKAPSDKLNI
                     AGVGIGGKGHPNIAAMSSENIVALCDVDWKYADKCFRAFPGARRYRDWRRMFDEHGSS
                     IDAVMVATADHTHAVISATALTLGKHVYCQKPLTHSIYESRLLTRLAERYKTATQMGN
                     QGNSGDGVRQLCEWIWNGEIGEIREVHTWTDRPIWPQGIERPKKVMPVPKTLDWDLFI
                     GPAPMRPYNEIYTPWNWRGWWDFGTGAFGDMACHILDPVYRALKLGYPEKVRGSSTAF
                     NTESAPQAETVEFTFPARDNLPRLALPPVKVYWYDGGLYPVLSDILPEGENLMADGLG
                     GCIFVGSKDTLICGCGGFNPRLASGRIPEVKPWLRRIPGAVGYVDGPHEQDWIRACKE
                     APESRVETSSNFMYAGPFNEMVLLGVLAIRLQSLNKSLRWDGENMRFTNISGNETLKV
                     ITSDEFRIIDGHPNFDTKYATFNASEAANEFIMHTYREGWSLPDMPA"
     gene            complement(46173..47537)
                     /locus_tag="PK288_00740"
     CDS             complement(46173..47537)
                     /locus_tag="PK288_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HPM17470.1"
                     /translation="MKKILLLTGLLVSLLSYSSTSDFPLKISSNGRFFTDQSGKPFFI
                     NADTPWSLFVALDSNETACYLKNRKEKGFNTITVNLLEHWFNGETLAYPEASRNKAGN
                     YPFTRYIEGNIPDFTFPDEEYFRHVDRVLEMALAHGFLVMMTPAYMGYDGEGLQDGWY
                     REVLANGPERCRKYGQFLGKRYARFPNIAWIMDGDRNPDQFSRPLETEIVRGIREFDK
                     IHLFTAHCHPANSSRDHWEGADWLDFNCVYTYAFISDNSTVHAECLRNYLMDPPMPVI
                     LFETTYENEQNANSLQIRSQMYWGWLCSIAGVQLGNLPLWRLGEGWQYAMDWQASYDA
                     SNMKKLVDSRRWWKLVPDLDHSTVTEGYGSKNSYVAAALADNGETAVIYIPSGAGILV
                     DLKRISGKRVIAWWFNPGNGLSKKAGEFTDKRETRFTPPDKRDWVLVLDDGDAGFGPP
                     GKSQ"
     gene            complement(47655..48278)
                     /locus_tag="PK288_00745"
     CDS             complement(47655..48278)
                     /locus_tag="PK288_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPM17471.1"
                     /translation="MNANHVITIGRQLGSGGREIGQKLSELLRFSFYDKELICIASRE
                     SGLNEKYFEKADEKKRFSLFGSISGFRGLDATHTMNYYLTDETLFKIQSDVIRKLAAQ
                     KSCIFVGRCADYVLKDNPGILSLFITANIDDRIKRVAALQNLPENKARTHIERTDRER
                     ARYYNYFSGRDWGAAEHYHLCINSSVLGIDETVEVIGRLAQKRFGLR"
     gene            complement(48301..49653)
                     /locus_tag="PK288_00750"
     CDS             complement(48301..49653)
                     /locus_tag="PK288_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPM17472.1"
                     /translation="MAQMRSPLALGTGNVWNLLLQYAIPSVIAMTAASLYNITDSIFI
                     GHGVGALALSGLAITFPLMNLAIAFGTLVGIGASTLMSLRLGQKDYESANSILGNVFV
                     LNIIIGLSYTMIILLFLDPILYFFGASEDTLPYAHDFMVIISLGNLITHMYFGLNAML
                     RATGNPEKAMYSTISTVIINIILAPLFIFVFGWGIRGAALATVLAQASMLTWQISFFS
                     NRKNFIYLKKESFRLKRKIVTDSMSIGLAPFLMNAVGSIIVIVINQRLIRFGGDLAVG
                     AYGIINRVAMLFAMIVMGLNQGMQPIAGYNFGAGLLDRVGRVLKLTIMVASGIMVVGF
                     LICELFPHGVASVFTTDSELIDLVVPGLRIVMFFFPIVGFQMVTVNFFQSIGMPGKAI
                     FMSLTRQVLFLLPGLIILPLFYGVNGVWYSMPLSDLLSSLISLYMLIVQYRKSVIKSV
                     "
     gene            50072..51094
                     /locus_tag="PK288_00755"
     CDS             50072..51094
                     /locus_tag="PK288_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HPM17473.1"
                     /translation="MKYRKGIFKWVWTFSLTMLVMSGCMKDVQKPEVTTGTVSGVLTT
                     SALIEGNTTGDGGGRVIARGVCFSNYIAEPYILKDNLFYQGSGAGSFNVYLENLTPGT
                     TYYARAFATNAEGTGYGQVVTFTTRGSMTGAITFNPDTVYNTVTDITGNVYKTIRIGG
                     KTWMAENLRTSSLSDGTAIPEVKAGEEWETTQEPAYCWYLNDDKYKDIYGALYNWHTV
                     ASDKLCPEGWHVPKYDEWMALFSHFGNNYEVADKLREAGDSHWVKTGPNVRNESGFTV
                     LPGGWRNIDNINFGDLGYAGTFWAVPQTGGDINTVMFFFMGSWEGIFNARNPGNAGLS
                     VRCVKN"
     gene            51289..51765
                     /locus_tag="PK288_00760"
     CDS             51289..51765
                     /locus_tag="PK288_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17474.1"
                     /translation="MLRYYRKLTRELIERSFPLLKGKKILIGMAPLNFYAFSIWLPPF
                     MRLMVFSTKTRRFDKPAIKGLVVHELCHQERYMRIGALKYLGFSVKYVVSRKARAAEE
                     KSTDRLTIEKGFGWELFRLTEITHNDRKNKKTNDLYMSPGEIRSYSENLGKWNAAS"
     gene            complement(51923..52681)
                     /locus_tag="PK288_00765"
     CDS             complement(51923..52681)
                     /locus_tag="PK288_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17475.1"
                     /translation="MKQIAAICLLAATAFSCQNDQTVVKTHLELANLKGSVRMVDKTV
                     HNAEAKSVCPAADKLEANQSTYVYNRSGNLEESSMIDPNGRVTEVSKYIYNKRGRCIG
                     IDKFSGNTKTGREVPVLDGGKMTELKIYDGDNTNIITYRYIYSGDDISVTRTIDREGN
                     ESSSVTNEYVNGELVSQTERDGNGDIKSISRFVRNPHNDVTEYIISVPKNNEEYRFNF
                     EYEYDEAGNWVRQTQYYDGRIVNIVIRNITYFEV"
     gene            53161..53523
                     /locus_tag="PK288_00770"
     CDS             53161..53523
                     /locus_tag="PK288_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17476.1"
                     /translation="MKIHFEHHTQKVLPFSRFLLRLGKYFVFSSVLIAFSLVLGMAGY
                     HFAGRLNLLDSFHMACMILTGMGPVVELTSPGAKIFSSLYALYSGIAFLSITAVFLSP
                     IIHRILHILHVESENRAR"
     gene            complement(53742..55517)
                     /locus_tag="PK288_00775"
     CDS             complement(53742..55517)
                     /locus_tag="PK288_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM17477.1"
                     /translation="MKLYSPISAGRGALLIILAGYLLFSSCSEDLLNKYPLDQITSES
                     FFSSAEDLELYANRFYPLLTADAGYFFTIMIDQNSDNMAPSAFDPRLAGVRTVPPSGG
                     DWEWSDIRQANYFLEHCNKAQGAESEINHYIGEVRFFRAYLYFNKVKIFGDVPWVSKP
                     LTTNSPELMGPRTRRDIVVDSILADLDVAISYLKSVEEANPFRINKQVAMLLKARICL
                     YEGTWEKYHQNDPFGVQGSDGSALIQKAEATARQLMDMNIYSIYKGPAGDEYRSLFNQ
                     LDYSGNPEVMLWKHFDLSLKIVNNVSRSLSGGGGNTGVTKGLIDSYLCTDGKPISVSP
                     DFHGYGSLLEEFSDRDPRLSQTIYKKGDYQTQNGPGGAFIQFGLPPIDQNGPFRSTTG
                     YSLRKGINNEYTQQTAENIGTKGSIIFRYAEALLIYAEAKAELGTLTQADADISVNLL
                     RDRVNMPHLDINDIATDPRWDFPDLSPVINEVRRERRVEMAFEGTRWDDIARWKADGL
                     IVNKRPRGVKYIGSDLEGAYKDANGNPTITIGVNLFVDEDGFVDPYQAFLPNGYKFNP
                     SRDYLAPIPSNEITLNPNLKQNPGW"
     gene            complement(55530..>56352)
                     /locus_tag="PK288_00780"
     CDS             complement(55530..>56352)
                     /locus_tag="PK288_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17478.1"
                     /translation="EQGEREKLVRFVEFMLADNISKVLDYYNPQGILSAPFYKGKYIG
                     EIWGYKTAGLFQSDQEAQEVDQSYLSKEPWRAGDVHYTDLNSDNKIDIGKNTVDDHGD
                     LMIIGNSTPRYSYSLLLNGSWKGIDLNMLLQGIGKRDLWLSGQQFWGAAGGLWNATAF
                     EEHMDYWSPENPDGYFPTPYLGKGSKNQQVQTRYLQNAAYLRLKSISIGYTLPASLVQ
                     KAFIKNLRIFIAGENLLTFTRLMKNFDPEGTFGYYGSGKSYPIQMTVTSGVNVTF"
BASE COUNT        15268 a        12966 c        12941 g        15177 t
ORIGIN      
        1 gatatcccct ggcatatcct catgacaatg aggaaagcaa aggatctgga aaaaataatt
       61 attgtcgtcg atagtgtgaa caataataat ccggcggaaa acactttctt ccttcccact
      121 atgtcagcaa gcctgccgaa aggtatcagg aaaattgccg acgacaggat aaagctgctt
      181 attacccatc ccagttcgat tgcacttgcg ccaagatcct tcccgattga tggcaaagca
      241 agatttacag atgatccgag gaatggggtc aggaatgctg caaaagctgc cacgatcaga
      301 acagctttct taagctcctt ttccgaagta gtctcatggt tattatgcat cggattcaat
      361 acggacaaat taaaaagaac actattcctt ttccctggtg tcaattataa tagtcaccgg
      421 gccgtcattg acaagttcga cctccatcat agctccaaac tctcctgttg caatctcttt
      481 tccaagcagt tcagatattt tttctgtaaa actgttatac agcgggatcg ccttttcagg
      541 aggggcagct tttatatacg aaggacggtt accctttttt gtttttgcat gaagggtgaa
      601 ctgactgatg accataatgc tgccattaac gtccctgact gaaagattca ttattccctc
      661 attgtcgtta aagatcctga gctgaacaat ttttttgcat agccagtccg tatcagtatc
      721 actgtcgttc tcttcaatcc ccagcagcac aagcagtccg tgaccaatag aggatttctc
      781 agtatggcct attcggaccg atgccctgct tacccgctga attacggctc tcatttttga
      841 tcagttaata aaattatctg gaatttttta tcgaattcag aatgaaatga cagtcccgtc
      901 atacgggttg ttacggctgc aggcaataat tcagcaggcc ggcccggatc agggttaacc
      961 acccggatcc cggatatttt catgtctggt gtgccggtca gattcatccg ttcccggttc
     1021 cggactacag catgaatgcc tgaagttgtc ataaccaacg atattatcag ggccggtact
     1081 gatttaaaac atacccgcaa atcaatttga aggctttaaa tatagtatct gttgaattcc
     1141 gatggaaatg aatatgctat atttacggaa ttatttttca gggatggact taataacaat
     1201 cgtagcagtt gctgccggac tgtcgttcga ttcatttgca gtgtcgctgt ccctaggtgt
     1261 tgtaaggaac aggattttgt tgaaggaggc cgtttccgtt gctcttgtta tggctctttt
     1321 ccaggcaagc ctgctggtcg caggttattt cctgggttcg ctgttaagcg atgtactgac
     1381 tgctgcggat cattgggtgg cacttctgct tctgacattc ctgggagcca ggatgatcat
     1441 tgaaggtctc agatccgaaa aagaaaaaaa acaaagagat taccacaatc cgtttacaat
     1501 aattacaata gcggttggaa caagtattga tgcatttgca gttggcataa gctttgccct
     1561 gcttaatatc cggatctggt gctccgccct ggttattggc atcgtgacat tcattgcgtc
     1621 gatgctggcc atcggaatag gaaaatcggc gggagcccat tttggaagga atgctgaaat
     1681 cctgggaggg attattctga tactgatcgg aatcaagatc tttgtcgagc atatgttcag
     1741 catataaaac aggactataa cgtaacataa tgcaatcaat cccctgacag ggactgttgc
     1801 gacgcggagt tccccctgat cctgatcagc ggcagggatt tccgggtttt gtcagaaagg
     1861 tttgctgacc tggacagggg ttggaacact gatcggcagg tatcagaaag ggatcatgaa
     1921 tctttccctg tctttgaaaa cggccatttc ggtataccct gtatttctga gcagttcata
     1981 ggcttcatcg aagtactggg ccacccttga tggcatatgt gcgtcggagt tgacgcatac
     2041 aggtactcct tcttcccgga aaaactttaa atagcgcctg tcgggataaa aatcggcaat
     2101 cggcctgttc ctgccgtttg tgtttatttc aaaggcaaca tcatgttttt taaaactggc
     2161 agccagcttt ctgtaaaggg gctccaggtc gaatgatggt gtgaaaccat aaatccttat
     2221 caggtcggga tgcgctatta tatcaaagag cccggaagcg gctgcggatg caacctgttc
     2281 aaaataaacc acgtacaatc tgtcgatatc cttgccggca taaaactccg gtcccatgtc
     2341 gacggtggat tcgccgagga aatgaacgga gccaatgaca taatcaatgt cagccttttt
     2401 aagaaacgaa ttgatttcct tttcccttcc ctcgatataa tcaacttcca gtccggttcg
     2461 cacagtaata tcatggttat tttctcgaaa caccttcata tgttccagat atctgtaagc
     2521 tttcctggca ttcatgctcc agtcaagatt tttttcaaaa agggtgaggt gctctgaaaa
     2581 tcctaactct ttcaatcccg cttccagggc cggagctatg taatcctctg gcgcagcccg
     2641 cccgtcgctg aaccacgagt gcacatgata atctgttttg taaatcattt gttaccggat
     2701 ttcgaagttt taacaaagtc aaagccgctc aagatactta ataaaacaaa ttcccggtaa
     2761 gaaatataaa ttggaaaatg ataactttgc gaaaaaattc ttggaagtgg ccaaaaaaga
     2821 cagatcctgc gaatgtgaaa aatgtgattt cagggaggtt gtttttgcca gccttgatga
     2881 acaaaatata caggctcttt gtgaaatcaa ggaggaaagg cattttcgca agggcgagat
     2941 catcaattat gaaggtgaaa ggattacgga tttcaaatac cttaagacgg gacttgtcaa
     3001 gcttttcaga cggtcgtcaa acggcgagga gcaggtaatt acaattacca ggccgtttga
     3061 gtttgtaagc aacatgagta ttttctcgga agaaaggtac aagtactcgg tttctgctct
     3121 tgaagagtca gtggtctgtt gtgttaaact tgatttcatc aaggaattgt tcattaaaaa
     3181 cgggcagttt gcaataaacc tgatgtcaaa aatttcagta atcaatgaca agatcataaa
     3241 tcagaccctt gatatccgcc agaaaaacct ggcaggcagg gttgcctatg ttttgctgta
     3301 tttcaccaaa gagatttata aatcaagggt ttttgacata ccggtttcca ggaaagaaat
     3361 agctgattat atagggatga gtacggccaa tgtcatcaga accatgtctg atttcaagaa
     3421 agataatatt atcagggttt ttggcaaaac cattgaaatt gctgacattg ataagcttga
     3481 aatcatatca aagagaggct gacttttccc catgggaatt ttccgcaaaa tacttatctt
     3541 taaagcctta aatcgaactt aaaaatggta tcaagtaaaa agcagaagaa catggcaaac
     3601 atcaaatcaa caaagggttt aatagctatt cttactggag gcggtgatgt tccgggattg
     3661 aatccggcga tcagggcggt tacgatccgt gcaaacaggg aaggatacaa ggtcattggc
     3721 cttcgcaggg gatgggccgg tataacggaa ttaataaggg atcacaaggc tgacaacagc
     3781 aactgttatc aggttctgac cgacgacatt gtcaacaagg ccggacgtac aggcggaacc
     3841 ttcctccata catcccgtac caggccaagc catatgtcaa aggctgcagt tcccgatcat
     3901 ctgaaggata catacaatgc cgatatgaat gatctgaccc ctgaggtgat caagaatctt
     3961 gaatggcttg gtattgatta cctgattccc atcgggggtg atgataccct tagctacggc
     4021 gttcatctct acaaacaggg agttaaggtt gtggcaatac ccaaaacgat ggacaatgat
     4081 gttcccggga cagattactg catcgggttc agtacctgcg tcacccggac aattcagatg
     4141 accaacagcc tgaggacctc ggcaggctca catgaacgct ttcttgtcct cgaagtattc
     4201 ggaagatacg caggattcac ggcaatgctt ccgaccatgg caggtgcagc caacagatgt
     4261 gtcatccccg agtatgaatt tgatatcgat ttacttacaa aacttcttgt ggaagacagg
     4321 aatcataatc caagcaggta ttccgtagtc ctcgtttcgg aaggcgccat gttcaaggga
     4381 ggcgaaatgg ttttttcggg aactgaaaaa gatgcctacg gacatgcaaa actgggagga
     4441 attggcgacc ttgtttccga aaaactgaaa gagtattctc caaagtacaa taacggaaaa
     4501 acaatcaaca tcattaacca gaaactcggc tacatggtca ggggtggtga tccggacgca
     4561 ctcgattcca ttgtaccgat ggcatatggc aaccttgcac tcgatcttat tctcaaggga
     4621 atgcatggaa ggatagtggt cctgaaaaac ggccggtatg ataatgttcc cattgatgtg
     4681 gttacctcat cgaaaaaaat agttaaagta tcagaacatt acaataccga acgactcagg
     4741 cctttctatc acagcttcga gatgaagcct ttccttttaa tggcttccga cagttagtta
     4801 taaaacaaaa caggaagaaa tatttcagat cagctgcctg tgctgatcag gaatatcgta
     4861 acagtcagtt cagtcagcag gctcgcgaag tcctggcaga ttgttcagta ttgatgtcat
     4921 ggtgcgggac accagcagtt tcggattttt taaatagtgt ataaatcgtt gcatctggca
     4981 gcataagtcc gccgggtata gtacagtatg cagttcaaaa tctgatctct tggaaaaagg
     5041 aaagattatc aagtctgttg gcagccgtta caaggtgctc cttgattccg gacgaacaat
     5101 tgactgcctg ctgagaggaa aactgcgtgt gaatgagatc aggagcacca atcccattgc
     5161 tgtgggcgac aacgttgaaa ttgagctgtc cggcacttcc ggtacgggaa taataaccgc
     5221 ccttcttgac aggaagaact atatcctcag gaaagcatca aatctttcga aatcgtcaca
     5281 gataatcgct gccaatattg accaggtcct tttaatgctg acaattgctc ttccgcagac
     5341 ccccctggaa tttatcgaca ggtttctgat aaccgccgaa gcatatcgga tcccggcacg
     5401 actggtcttt aataaaactg atctttacgg gaaggaagaa atggaacgcc ttgaccaact
     5461 tgtcagtatc tataccggca tcggatacgg gtgccacagg ctttcgctcc gcgacggatc
     5521 cggcttcgac gaactgaaaa ccgtgatgaa ggacaagatt aacctgattt caggaaactc
     5581 aggagttgga aaatcaaccc tgcttaacct gatcgatccg gcctttaata tcagaacgga
     5641 agaaatatcg gattttcaca aacagggaaa acatattaca actttcccgc aaatgtaccg
     5701 tttaccattt ggcggttttg caattgatac ccctgggttg agaggttttg gtattgcaga
     5761 tatggaaaaa aatgaaatat accatttttt tccggagata ttcagaaagt caaaagattg
     5821 cagattctat aactgccttc acctggatga gccggaatgt gctgtcagga aagcagttga
     5881 aaaagaagaa attaattatc tgagatacag aagctatctt aacatcatgg gtgacgacaa
     5941 tgccaagtac cgctgataca actaaaggat tcattctttt gcggcaaaca ggtatatgaa
     6001 gatgaataaa attttatggg ctgtatctgc agcactgctg cttattgttc ttgcatcatc
     6061 cctggttttt tccggcctgt ataacaagca gatcagttat ataaaaaacc ttctcgaccg
     6121 tgaggtcaga attgccggtt catctgtcga ccagatcaat aatgaatttt ccagtgatct
     6181 gaatcagata ttgttctcgg tggaccattc cctctttttt actgatcctg tacagcagat
     6241 gaagatagag gataagatca aatttttttt caccaaatat gaatccgtca ttaccggtat
     6301 caaattcttt gacaacaaca ggaacggcta tactctgaaa aaagatgaaa caggtaataa
     6361 ctggctcatt cacaattttg tcctccatgc ccaggaagaa atacattcaa gggatgagct
     6421 gattaagggt tcaagaggat atgaatatta catgccggtg ttaaaaaacg gcacactgat
     6481 cggaaatttc attgtatcaa tcgatttcaa caaatatttc aatgatctgt tcagcgggtt
     6541 caatgcaagg gaataccaat ggcaatgggt tatcagcgat tcgggatcag taatttacga
     6601 taactccggt caatccggga aatattcccg gattgaccgg cttacacggc ttgtggctga
     6661 gggtgcttcg gaaaatatcg ttcattcatt tgccattgaa ggaaatcata aaaagatcat
     6721 tacttcattt tattcgacac aactgtttca gaaagaaatt ggcctgatgt tctcagcgcc
     6781 ggctgacaaa ttcaaaaatt acatcctgaa agttacactg gcaatggccc ttcttgccat
     6841 agcattgatc ctgggtattt tttggctttt cgtacagcat atcagatcgc tggaggagaa
     6901 atcagccaag cttaagtcgt cggaagaaat gctgttcaga atgattgaca gcattcctgc
     6961 cggagtgata atctataacg ggaacaggga aatcatgatg gccaacaagg aggctgcgaa
     7021 ccagttctca taccaaagcg aatctgaaat gaaaggccgg atttatcccg gaaataaatc
     7081 aactgatgag tcagcctatt ttttaaagaa tctcgcaggt actttcagtc atgatcagtt
     7141 tgttatcata aagaaggaga ttggagaact cattctttac aggaatacca ttccggtttc
     7201 tttccagggg gaggatgcaa gcctggatgt tctttttgat gtgacaatga tggagtcagc
     7261 ccgcaagcag gctgccacgg ccaataccgc caagtcggag ttccttgccc gaatgagcta
     7321 tgaattaaga acaccgctga acggtatcat cggtatggcg gatatcctta ttaaaagcag
     7381 gctttccgct gaaaacaggg caatagtcaa cctgctgaga cagtctgcgg aagtcctgct
     7441 gaatattgta aacgacatat tggatttttc aaagattgaa tcgggcagga tgatactgga
     7501 tgagatcccg ttcagcctaa gggaagaaat cctatattcc tacgacctgg cccggaccaa
     7561 tatagatgaa aatcaaataa agctgacatg ttccgtggat gaggaagtgc ccgacaaatt
     7621 aatcggtgat ccgtacaggc tcaggcagat cctttccagt cttctcagcc attcagttga
     7681 caataccgcg cgcggcagca taaacctgaa atgcagcctt gcagaaaaac ataacgggac
     7741 aatgaatctc aggtttgaac tgaccgacac cggtaaaagc tttgacaggg ctacaataaa
     7801 aaaaatcttt ggggaacaaa taaatattga gtctaaagtt catggcgacg atgacggttc
     7861 gggtttcggt acgatactgg ccaggcagct gatcgagctt atgggcggga aacttaatgc
     7921 tgaaagtccg tcaggtcttg acggagacaa gggattaaaa atcagtttca ccatttccct
     7981 taacctgaat gaaacggtaa ttaaggatct tcagtttgat gatattacct ctttcgggca
     8041 aatcaggaca ctggtgataa gcggagggca tctgaaggat gaggagatcc tcaattccct
     8101 ctacaagctc gggctgactg tcagcgttac ctcctaccag aaatcgacga taaatcagtt
     8161 aatggcaaac ctttcattcc cggaaaaaag ataccatctt ctcgtaatag ctgacaacgg
     8221 ggaattcagc ggttttgcgg ttgcccagga tctgtgggac aataacctgt cggccaggtt
     8281 cattattatg ataataagtt caaatgatgc ccggggaaat ctgctcaaat gcatcaggct
     8341 cggggtggat aattatatcg tcaagccatt cgagatcggg gagctttatg ataagatcag
     8401 aatgaaattc agtgggacag acaggcaggc agacaggcag gaagctgaag ccggcagccg
     8461 caatatccgg atactggttg ttgaagacaa taagctgaat cagaaagttc tgggcacaat
     8521 gcttaaaagc ctcggatatt catttgattt tgcagatgac ggatttgccg ggctgatcca
     8581 ggccaaaacc aggaaatacg atgtaatttt catggatctt ctgatgccaa gaatggatgg
     8641 ttttgaagct gcccggcaga tcctggaata tgacaattca caacttatcg ttgcattcac
     8701 tgccgacaac ctgcccgact ccaggagaaa agccgaattg gcaggaataa aggaattcat
     8761 ctccaaaccg gtccggatag atgaacttcg cgaatttttc aggaagcatt ttttcaaaaa
     8821 ctgatattac atttgcatgc ttccacatga tcccgggtgt ttaagggaaa tgatggaaat
     8881 cattcaccgg atatgacatt tcagctgggg cagaaactgg atatcagcct tttccttgaa
     8941 cgggttgaac acattcctgt cgtggatgtg cgctcgcctg cggaattcag tgcgggtcac
     9001 atcccggggg ccttcaatat accgcttttt gatgacgaag aaagaaaggc agtagggatc
     9061 cgctacagga agagcggccg gttcggggca ataaatgaag ggttgaaact ggccggcccc
     9121 tcgttccatc tgaaactgga aaaggcacgc aagctttcag cagacaacaa actgcttgtt
     9181 cattgctgga ggggcggcat gagatcggaa gccatggcct ggctgttttc actggccggc
     9241 attgaaaccg gcattcttga aggcggttat aaggcataca ggcaatatat cctgaacagc
     9301 ttttcggaaa acaggagaat gattattctt ggcggattta ccgggagcgg gaaaacagag
     9361 atcctgagga acctcaggaa tgcaggggag caggtcatcg atcttgaagg gcttgcaaat
     9421 cacaaagggt ctgcttttgg ttccctcggt cagcctgcac agcccacttc ggagcatttt
     9481 gccaacctgt tgtatgaaca atggaggaga aacgacattg aaaagcctct ctgggtggaa
     9541 gatgaaagca aaaacatagg aacggtattc atgccggacg agtttttcct gaatatgcag
     9601 aaaaaccctg ttatcgtcct tcttatcgat ttcagaatac gccttcacag gcttgtgagg
     9661 gaatatgccg gatactccgt ggctgacctt atcctggcca taagaaaaat atccaggcgg
     9721 ctgggcggcg agaatgcaat ggatgccatt tcatcggttg aatgcggcga ttttacaaaa
     9781 gccgccgaaa taattctcag atattacgac aaagcctatc tgtacggatt aaaaaagaag
     9841 caggctgaaa aaatcgtgta tgttgaatca gactctgacg atgccgcgat caatgctgaa
     9901 agggtgcttg aggcatcggg gcagctgtgc cgtaactgat aactgccgga ggaatttcac
     9961 ctgcctgcct tcacccattt gtataccttg tctgatcttc tgaggtaatc cgggattctg
    10021 acagagcatg attctcccct gagggctttt tctgtggcca tgagatccac cctgatttca
    10081 tctgcagtgt attcaatcac tcctgtcgtt ggacccgtga taagcaggag gtcacctttc
    10141 tcaagatctc cgttctcaag ctttatttcg gcaaccttga gccttgtaaa gtaattgacg
    10201 acttttccaa ggtagacttt tttcattgtg gcattcgatc catagcgggt gttccactca
    10261 cccgacttct gccccaggta atatccatcc cagaagccgc ggttgaaaac agccgaaagc
    10321 ctctccatcc atatttcgat cttttcactg ccgtatgtac cgtcaagcac cgagttgact
    10381 gcttcattgt agcatgaaga aacaaacctg acatactccg gcgaccgtgc ccttccctcg
    10441 atcttgaaaa cccttacccc ggcctcgatc atcctgtcgg taaaaccgat ggtgcacagg
    10501 tctttgggag acatgatgta ctggttgtca atttcaagct gattccctga ctccttttcc
    10561 gttacaatgt atgatttcct gcaggtctgg tagcatgttc cgcggttcgc cgatttgttg
    10621 ttctcgtgca ggctcaggta acatttgcct gaaacagcca tgcaaagcgc accatggacg
    10681 aacatctcta tctttaccag ctctcctccc ggcccgcgga tatcctgttt gcggatattg
    10741 tcataaattt ccctgacctg ccccaggtcc agttccctgg caaggacaac cacatcggcc
    10801 cattgtgaat aaaatctgag tgcctcagtg ttggcaatat tgagctgggt cgaaaggtga
    10861 acctccattc ctgacctgaa ggcataatca ataacagaca ggtcggaagc tattatcgcc
    10921 gaaacaccgc tttcagccgc tgagccgact atcctgcgca tctcttcaat ctcattgtcg
    10981 taaacaacca cgtttacggt gagataactc cttattttgt tgtcgcggca gatctctgca
    11041 atgcttttca gatcgctgag tgaaaaattg acggatgagg cagctctcat attcagctgt
    11101 ccgacgccga agtaaacaga atcagctcct ccctggattg cggcgatgag tgattcatac
    11161 gatccggccg gagccattat ctcgatttcc tttcgatcca tacctggttt aagtttgact
    11221 aaagcgggtg atgatcaggc accgcttttt ctcatcatga tcctgatgca cgaacctttg
    11281 ccgatttcag agcttttgac gaaaatttta ccattgtggt attcatcaat gatccttttt
    11341 gaaagggaaa gcccaagtcc ccatcccctt ttcttggtgg tatatccggg attgaatatt
    11401 ttcttgtatg ccgtctttga catacctttc cccgtgtcag aaatatcgat aacggcgtac
    11461 ttttcatctt cagttaccct gactgtcacc tcgccgctcc cctcaatggc atcgatggcg
    11521 tttttacaga cattctcgat tacccactca aaaagagcag gatttaccgg ggcaagtatt
    11581 tcatcatcgg aagaaaaggt cagtacgaat aaaaccttgt ttgatgatct tgattttaga
    11641 tagtcaacgg aattctgaac aaggcttatg atattttcct ctgaaagttc tggttttgat
    11701 ccgatctttg aaaacctttc cgtaaccttt tcaagccgct tcacatcaag tgccagttcc
    11761 ctcgttacct ggatgtcggg gtagttgttc tgcaggatct ctatccatcc cgacagggat
    11821 gttgtcggtg taccgagctg atgtgccgtt tcttttgaca tcccgaccca taccctgttc
    11881 tgctcggctt ttctcgacga actcagggcc tggtaggaga ccaggatgaa catgatgatc
    11941 aggccaagct gaacgtaggg ataatatata agctgggtga ggatgatgct gtccttatag
    12001 tagatacggt ttatgaatcc gcctccaaga tcaattatta tgggattgtt ccttgacttc
    12061 attttctcaa ggctgacccc caggctgccc ctgtcacctt ccttacctaa gtcaatattc
    12121 ctgacagcaa taatgttgtc atgcccgtcg gtgaggataa ccggcaccgt gttgttgttc
    12181 tcaatgattg aaaggagaaa ctcaagattc tgggttgagt cggcagaatt gatcagacgc
    12241 gtggcttcag cccacatctg gacttttttg cgttcttcgc ttttcagatt ttttacaagc
    12301 gactgagtat agataagtga cccaactccc aggagaattg caaaaaggaa taaaaacacc
    12361 ttccacctgc ttctgttcca gtaaatattc aagagatgaa ttctttaatg taaaataaca
    12421 ctatttcttt ctaaaaaact tcctgattcc tccatctttt ttttctgatg aagttttttg
    12481 ttctgtaccg gttgtttcgg taccttccca ggatatcctg aaacggggct ttgaagccgt
    12541 ttccttcttt acctgctcag tctccgaagg ataccaccca tactcttcat tgagaatggt
    12601 tttcagcatc tgacgctcat tgcgcaggtc attctttatt ttgctgcctg ctgccttcat
    12661 gtcatatgtg acatcaacat cctccccttt gcccataagc ttcaggaaga cccgtgtccg
    12721 gcccagcccg tcatcctcaa cctcatcgaa ttcagatgat gttttcctgt ttttctttgc
    12781 tttcctgctc aggatctctg acagcagcat ttccacatga tactcgtatg cattgtcaaa
    12841 gctgtgcctg ccgtttaccg ccagatcgac cgcggatgac ctgatttgca tcaccggcac
    12901 gtaaaaataa ttgttgcgga tgaagaaatc attgtcaagc ttgtcaaatt taatattctc
    12961 aagttcggat aatgaaatga attttgacag ggcttttaca ggatcaaaat taatgagagc
    13021 gccgtctgaa agactgtatt tcccttcagc caccagcgat ttcattgccg gattgagcat
    13081 tgaatcagcc ggcaggacca gggttattgt cccggaaagt gaaccggcta tgttttcggc
    13141 cttcaggaaa ttctgtccga aattgttgaa ggatatgaag gattttttta catccacatt
    13201 gctcacactg aaactacccc ttccggtaaa ggatttattc ctgttttgca ccactatacc
    13261 atttcctgtt accgtgccgt cctgcgattt aaacctgagt gacctgaaat taagcattcc
    13321 gggtttgagg ctgatctcac cgtgaagctg tccggcatca aatttcttgt atttaaggga
    13381 atcaagttta acggtaagat cgatcatcac atcacgcgga agcgccaggg gagcccttgc
    13441 ttttacagtt tcgtcccttg tggccggact gctgaaaaaa tactcgggct tcaggcaggg
    13501 tgaataaaca taagcatggc cggtaagatt aacaggtttt ccggccagcc atttcggcag
    13561 attgtcgaga ttacccgaca aactgaattc ctggtcgttt aaattgaatt caagcttttc
    13621 tgctgaggtg ctgccggcaa ttaccagctt accgttagtg ttcctgagat tgaaattccg
    13681 gccgggaacc tctgtgccga atgacctgaa aatgatttcc gacctcgaat taagctcggc
    13741 aaggtctgaa agactgtaat gatccttttt ttcaatgaca cctgaaaggc tgatatcaag
    13801 atcaactgat ccgctgcacg gaccgagagc ttccatgccg gcaaactccc ttaattcgga
    13861 cggaataagg gtccccctga atttcagtga agccctgggt ctggagaaat ccgatgctga
    13921 aaatgatccg ctgaaaacgg atgaacccaa gccggctgtg aaatcactga tcccgagcgt
    13981 acttgtttca ggacggttcc tggccccgtt cgtcattgac gctgtaaacc ggacatcatt
    14041 cattttcaga cctgacctga cccgatgcag accgccggct tccagtgaaa cggcaatatc
    14101 gtaatgcgga tcctcaaatc gggttgactt tccctttatc ctgcagtcga gatcaaaaat
    14161 cccgtatggc ctgtactcag acacgatggt tttgtaattt cctgacaggt agtggaaagc
    14221 ttttgaaatg tcgatatgat tgccgctgat gctgagatca agataattgt catcgggaat
    14281 aatgagcccg gtcattatta tgtcctgttt atcaaagctt attgtactct ttctgaaaaa
    14341 gattcccttt tcgttcctgt tcagtccgac atccatccgg gcattgaaac tgcggttgaa
    14401 aactgtccgc ttcaggctga aatgtgtgaa cagaacgtct gagctgccat caaaatcaat
    14461 gtttgcgccc cttatcctgc tcctgatgcg tccgtttttt aaaaggcccc tgatcacaag
    14521 gtcggcccgg agatcactgt acaggaccct gacatcggaa acattgatcc tgtcaaggtt
    14581 caatcgtact tcccgttcag catcaccctg agtctccctt tttgtgaaac tgtagttata
    14641 ccggccggct gaatcggtga aaaggttaag gtttcccgac ctgacattga ctgccttgaa
    14701 ggaatagacc ccgcggaaaa ggtcgatcat tttaaagtca attgatgcag atgctgcagc
    14761 cagaagggta tcggtgttca tatcactgaa gccactcttc tcaaaacccg gcgacgaata
    14821 tactctgata tttttcagct cgaaagatgc tttggggaat tttctcagca gtgatagatg
    14881 atacgaaccg atctcgattt ttgttgcaag atttttgtta agtgccctga taataacttc
    14941 tgcaacctta tcctgcaagg caactgaaag ggcaaaaagc actatcactg ccgatacgac
    15001 aagtattccg gctaacttaa gaatcttttt cggaaatttc atctgcaaaa gaatatcctt
    15061 ccccgatggt aatgttaacg gaggacaaag ttaattattt gatgccagat taagctatcg
    15121 gagaagaaag gataaatcat caggttcacc cagttcggcc ggtattgtgt ctttcctgaa
    15181 tatcctggcc tttccgggac cggtaaggtt tatcctgttg tcaacaacat gcagcatggt
    15241 accttccctc aggccaacga cccatacatc cggattggct tcaatgaatt ctgcgatccg
    15301 ttgttccctt gtttctcctg catgtccttc cggactggca tctagataat gagggtttat
    15361 ctggaaagga acaagattga atgacctgaa agaagacggc cgggttatcg gcatatcgtt
    15421 ggttgtctga attgtcggac atgcaacatt cgatcctgca ctccagccta tgtaaggtgt
    15481 tcctgacagt acctttttcc ttaccggcaa tatcagaccg ttctgccgca acagtttgag
    15541 tagcatccat gtattgccgc cccccacaac tattgccggg gcatcagcta aggcgcgtac
    15601 agggtcggac cagtgatgga ttgaaatcac atcatggccg atttccctga atcgttcctg
    15661 tactttcttt tcataatcat cgtatgaaaa ggttactgca gcgtagggta caaaaagagc
    15721 cctgacgggt gcccggccca ggaaatccct gatccggtgc ttgggataat cgagataggg
    15781 ttcgccggca ttagttgaat tgctgataag caaaagtctc atttcggata atgttctgtt
    15841 aatttgattg actggtcccc tggtctgaag attgggtact tttggccctg ttaatagtta
    15901 tcataaagcc tcgtcagttt gcggttttca ggatttgctt taatggtgaa tatcccggac
    15961 ctgtagtttg caaggctgaa tatcagtatt ttgccggaga gtacctcgcc atagcccgtt
    16021 atgatcttta ttacctcatt tgccatgatc gtgccggtaa ttcccggcag tacacccagc
    16081 agcccggttt cggaaggtga aaaaactcca ttttccgccg gcgggaaagg gttgaagcat
    16141 ctgtatgtcg gccctcccat gtaattaaat acagctacct gtccctcatg tttatagatg
    16201 gctccgtgta ccattggttt gtcaaggata acacaggaat cgctgatgat gtaccttgtc
    16261 ctgaaattat ccgtggcatc tactatgata tcataattcc tgagtatgga caaagaattg
    16321 cgggaatcga ttttcaggtt aataacgact gtatttatca tacagttcag ttgttcaatg
    16381 cgtcttgcag cgataattga tttcagcttt ccaagatcat cggaaccgta aagcacctgg
    16441 cgctgaaggt ttccctcatc aacggtatca aattctgcca gggcaatttt cccaacgcct
    16501 gcagctgcca gatactggag aaccgggcag cccagtcctc cggcgccgat aaccagtact
    16561 tttgccattt tgagcttcag ctggccctcc tttcccgttt caggaagcat tatctgcctc
    16621 gagtagcgcc tgagttcgcg gggtgttaaa atgaaatccg acattgtatt ttacgatccg
    16681 ccctgaatta tttgacagga tcggtaagat acaaaaaata atgacggata ccctggctga
    16741 atatttgttt tctggccgcg gcgggtcagt cgttgaaggt aaatgccagt ccgatttcga
    16801 aaggttcgag gttcatgcct tccggacccc tgtttttctg gaaccagggc gtgggatagt
    16861 atgtaccgta aatcatgaaa ttcctgtatc cgacccggcc ggtgaatcct ccccggagca
    16921 ggttcaggtt ataatcaccg tttacacgcg acttttctcc gtttttatag acgattttcg
    16981 tccaggcatt cagcttgagc cctcctgtta cccctgctgc aagattaagc ctgccgttgt
    17041 aacctgccgg tatctggatc tcaaacaaaa gcggtacatt aagataaaga gttgaaaact
    17101 tcgatcgcct gaccggaacg gaagaggccg gaagttttcc ggttacggtt ccttcctcac
    17161 cgacttctat ggtagtgtga ttcctgaaac ggtaattgtt ccagttgagg cccatgccgg
    17221 taaccagacc cgaatatctg ccaaaaccta tgttcagctg cgaaaaattc agattgaacg
    17281 accatgagtt gcttgtttca ggcgacatat agtcgatcgg gcccggcagg accattgaac
    17341 cttcatgaat atagttattt aagccggcct caaaccccgc aagatgaccc ctgaaacgcc
    17401 ttgccttcag aagccagctg cggtcgtcat catccctgaa atgagtcccg ctttcttcat
    17461 cacactccga ttcagtatcg tatttttcaa attcaacctt tttcccttcc agggattcaa
    17521 ggatctttat ccctctgctt cccagcctta ccgtgacgga tgaatcattt tcatcaaaac
    17581 tgaagaaatc tttacccacg gcaatgttca tgctgccatc cgcctcttca atcactgaat
    17641 gctgcctgcc ggcttttgcc gtgtcatcag catgaatgcc cggagcctga aggaaagcca
    17701 tcagggccgg aaatatgaat atgaatgttc tcatcttgtt tttttttgat ttcagttatg
    17761 ggacgaacag gtattgcaaa tagttacagc tgacttatgg tttttttgca ggagcattga
    17821 atttcaacaa ccctgaagag aaataatagg atcttaattc tccttttcca tcggttattc
    17881 tgtcgagagt catatcccat ccgagaagcc tgttgatgcc tgtgatgctg acaatggcca
    17941 gatcgaagga ttttacaggc cggtcacccg gtaaggtgtc attcattatg aaaccatgaa
    18001 caagccttgc aagaagcctg tcaacattgc ttctgtgaac cggatattca ggaaccaggt
    18061 tcgaaggatt gaattttctg aggatttgtg aattgggatc cggcggagca accggtatta
    18121 aagttatgcc tgcagcaagc ggggccgggg gagcgggcag ggaagccgga attgcccggg
    18181 atgattcatc agttgtcact tgtactgtct ggtctgccgg tggtttttta tccgcaattt
    18241 ttatttcgtc cgtttttgaa ccggtttccg cttgctggtt tgaccggctg cctgtaatca
    18301 ggcttttctc ttcattcact gcgttatggc cggctgatgt gccggattcg gcagttaacg
    18361 gactggttgt atggttttta atgacttcag ccgaagggct gaccaacagg cggaccgtga
    18421 tcaggagagc aaccatagca gccatgccga ggccggcaag tgaatatctg aagatccttt
    18481 gccctgctgt taatttaata acagagcttt ttcgcctgaa aacagctggt tgcggcctga
    18541 ctttaagcct ttttatcagt tcaaaggtct ttctcctgtt atcatcatta ctgatgatat
    18601 tgtcaagttc ttccagctgt ccggccgaca ggtcgttttc caggccggca atgcacataa
    18661 cgacaaactg ttcttcggaa atgctgccgg gggatttttt caggacgtct ttctttgcat
    18721 agcttatatc cggaggcctg aggtttacct gtttcagttc gggcaatgtg gcggcaagac
    18781 cgggattttg ttcgagaaag gtttttaaaa cctcttcctg ccggggatcc agattccctg
    18841 caagccagtc agcaaggagg atctcaaaat cagatctttc aggtgcggtt ttcataagac
    18901 tgtttctact ttcccgataa aattcttcag ggccatcctg cccctgtata tatttatttt
    18961 tacctgtgct tccgtgaggc cggtaatttc tccgatctcc ctgtaactgt agccttcgta
    19021 atcgcgcagg aggatcagtg atttctgtat atgcggaagg cgttcaagag ccatgtgaag
    19081 gatctcgttc agatcggaat actgcgattc atcaatcaga tcatttccgg ttgcgtcttc
    19141 cattggcgac attctgcttt ccttgcggat aatgtcaatc atgatgtggt aggcagtggt
    19201 gaagagccat gatttaacca cgggataact tatttccatg acattccgcc acagtttctc
    19261 atacgtatcc tgtactatgt cactggcctt ttcttcatcc ctgatgctgg ccaggatgaa
    19321 cctgtacacg taatctgaat attcctcaac agatctgtta tattccttta ctgtcatggg
    19381 ttgaaataca tgataaagac ggatgaaaac cggaatagtt acaggcatat taaaaaaaac
    19441 tgacacgcgg tcatggcgtg tcagtgtaat tacagatgac tgatcggatt ttaactgaac
    19501 tggtagatcc ttacccagtc gatttccatg gtgctcattc cgctcagggg tttttcagta
    19561 ccccctgaca ggagtacata catgggctcc tggggaatat ttgatgtctg gcggaataat
    19621 acttccccgt ttattttcca gacaagcatg ctgctggtcc attcaaggga aaaaatataa
    19681 aaatcgcggg catagcgtga accggccagg gttttgagtt taatctgcgg cgaaggtgaa
    19741 aaatcggccc atacctttcc cccggaagtt ctgcatatat cgatatgagg tgtcatttcg
    19801 ccggcaagca tccagaatgt atttctggca tccgggttac caagttttat tttggctgta
    19861 aaaatgccat atttctgacg gaagctgata ccggaattaa tgattccgga agtataactg
    19921 aattccctgg tccggaatcc caggtcggga gaccatactt ttccagtgat ccgctggggt
    19981 ttggtatgga ttctgaggag gctgttacgg atttcaaagt tatccctttc agtgtaggcc
    20041 tgcaggtccc cttcaactga atatctgtct ttcaacagtt tgtcgcccca gtaataattt
    20101 gtcagccatt tatcagtatc aagcttgtct ccttcgaatt catcggcaaa agtcagtgtt
    20161 ttcttcttca gtacatcaaa gcggtccgaa tctttcattt taaaatacca gacaatatcc
    20221 tcatttttct gaagtttttc gaactcactg atttccctga acatatctgt cttctcaaat
    20281 ctttttttat caagaagata gtttttcctg tccctgaact catttgttgc caggtattca
    20341 cgaagcttgt tatacctgtc gagccgtgaa gacccgtcgg tattcaggaa attggcatat
    20401 tcagccgaag atttgaagtt gtagtattcc ctgatctcaa tgctgtcctt taatctttta
    20461 tattcgagaa gcttcttgta ttcttcggta tctttgaatg ttatgggctt catgttcttt
    20521 ttggcaagga actcgggtgt ttttatgtag gctcccagtt cctcgaattt ttcaacagcc
    20581 tttgatttaa ccgcttctcc ctttgccgat gccttatagt aagccctgat ttccggatcg
    20641 gatttgaggg atttgtattc caggaacttc cgatattcat cagtgtccct gaaagtgatg
    20701 ggtttcattt ttttcttgag cttgaaaccg ttggatctta taaaggtttc aagcgcttca
    20761 aaatccctta ttttctgcga tccgtcaagc tccttgaacc gtttgagcat gtttcctgca
    20821 gaggttttga aatacattac gatatccttt gctttctgaa gcgacaaaaa ctccttttcc
    20881 ttgttgtact cctcggaatc cctgtaacgg agtgattcga tatctttctt tttctgtata
    20941 aattcagaag atgtcacttt ctccctgaga ccggtaaatt gagccagaag atctgacctg
    21001 gttaatgcct gcagtttttc gtaatcagca ataagcgcct tttcagcctg ttcaattttt
    21061 gatgttgtgg ggatccagcc caatctgagt ctgagacttg ccatctgcaa aaatttttag
    21121 atacaacctg ttaaaattaa aaatctttcc cttataaacg ttttggccaa tgcaaatatt
    21181 atcaaactga caaatacgta tttatgccgg aatgataatc tttgccgccg gggctgtaaa
    21241 atgcccttca ccggcagaac tgccgcaact gcttttatgt tcaataactg aaaaagttgt
    21301 cttctccaat accaacgtca aaacggccct gtgtaacact aaccggttca tctttaatca
    21361 gggcctgaaa ggaaaacact cccgatagta tcacttcagt ctgctttgaa tccacataga
    21421 gaagctgaac tctcctgaac tcgagttctc cctggagtat atcaggttta atggtatcac
    21481 cgtcgaggaa taatttcacg gcgcccggcg tattcaccag gtcaattatc tttttgtcga
    21541 ggattgtaag atcagcatat gaatcaggca taaccgacgg aagcaggaat gtcaccgaca
    21601 tttccttcct gttgtcccag gaatggtagc cgtagccgtc tttctgacct ttgagcacaa
    21661 acgaggtgat tcccccggca cggattattt tggcagggat cgcattttca ctgtaaagga
    21721 acaatcctct ttcataatat gcgccgaacg tattgtagcc ccattctgta taagcaggaa
    21781 gctcagggaa atcagggtct ggaatgaaaa tcgatttatt gagttcatac tccttgctgc
    21841 atccgtttgc aaggattatg gcagcggctg caatgagtga ataaacggct cttttcatga
    21901 ttgctttttt taaaacggga tccttgcatt taactgtatt cctgttccgg cttcaggagt
    21961 cacaaagtcc tgctttcctc ccctgatcat ggtaacaggt atatgtattt ccacattgac
    22021 ccccggtttg tcaaccattc ctattctgaa ccccacgcca aggtgaaagg atgaataaaa
    22081 gacccacggt ttttcgatta tattctcaat ctcatcttca aatactttca tgtccctgtt
    22141 gaaaaaaacg gtccttgccc tggaatgcag cctgtaattt acatcacctc caattttgta
    22201 gtatacattg aatgcccttg gcctgtaggg atatatcctt atctcaaggg ggatcgcgag
    22261 gtatcctgca agctgggtta tttcattcac tttcaggtaa tcggttctgg tttcctcctg
    22321 gcggagcagt aagtagaaaa aatcagagct gtttgaccaa taggattcct ttccgattga
    22381 actttccgtc cttgtatacc ggataccgct caccaggccg accttgttgt tcagtactgt
    22441 cttctcgccc ttgatgctca cgtaattcct gtagaacagc cccctcaggc tggttgttgg
    22501 aaaatctgaa aaaaactggc ccctgtcagc cctgatataa tccttttccg gcgggccccc
    22561 gctaatgaaa tcgatgccgg cgtcgacgga caggcacctg gtttttgtgt cctgacttaa
    22621 acctgaaacg gcaacaatgg ttgaaagaaa aactgaaagg agtattttta gcttcatggc
    22681 actgcagata gattatcaaa gatagtacat tgagttcaga taacgcggaa gaatcaatta
    22741 tattcggaga tcaggcggaa taatcctgat ttgaaggata atcacgggaa gaaataagcc
    22801 gtataccagg ttgcgggaac gtttttctgg tttatgcatc ggaaacaaga attaagtata
    22861 ttagcgcggt gaaattcatc atccggcaaa cttacaagtg atgacagatt tttttgatca
    22921 tctgatggac gaatttgtac tgccgttgca aaatccggtg ctggtttttt cattgatcct
    22981 gtttataatt cttatctcgc ccatactgct caggagattg aatattccgg ggataatcgg
    23041 gttgataatt tccggtgtta tcattggacc tcatggcctg aatatcctgg caaagaattc
    23101 cgcgattgac cttttctcaa cgataggact tctctacatc atgttcattg ccggactcga
    23161 gctggatctg aatgaattca aagccaacag gaacaaaagc ctcctgttcg ggctgtttac
    23221 ttttttcatc ccgcttgcca tcggataccc ggtatgcttc tatctgctgg gttatgattt
    23281 caatgccagt ttcctgactg ccagcatgtt tgccacacat acgctcgtgg cctatcccat
    23341 tgtaagcaaa cttgggatct caaaaaatca ggctgtcgcc ataacggtcg gcggaacgat
    23401 tttaaccgat acagccgttc ttataattct ggcagttatc atggggaaca gccagggaaa
    23461 cctgagccag gaattctgga tccgcctggg aatatcgctg gcgatcttta ccgctattat
    23521 gttcctgatc attccgagga tagccaagtg gtttttcaga aaactggaaa gtgagaaaca
    23581 ttcgcattat attttcgtac tctcggtagt gtttttttca gctttcctgg cagaagtggc
    23641 cggtgttgaa cctataatcg gggctttcat ggcaggactg gctcttaaca aactgatccc
    23701 gcattcatcg gccctgatga accggattga gttcatcggg aattcactgt ttatcccgtt
    23761 tttcctgata agcgtcggta tgcttgtcga tgtgagggtc atcctgaaag gacccatggc
    23821 actgatcatc gccggaaccc tgtcagtggt agcattaacc gggaaatggt tagctgcttc
    23881 attcacccag ctcgtatttg gatattcaaa aaaccagcgt cagctgatct tcggcctcag
    23941 cggggcccat gcggctgcaa ccctcgctgt tatcctggtg ggattcaggg ccgggatcct
    24001 tgatgagaac atattgaacg ggacaatcat acttatcctt attacctgca tcgttgcatc
    24061 ctttgccact gaaaaagccg ccaggaaaat ggttgttgaa gccgaggaag atgacgcggt
    24121 tctggccact gcaaacgggg ttcacaatga acatatattg ctgccaatcg caaatctgga
    24181 gaacatagaa aaactgctgg agttttcaat atatatcaag gacaaaaaat caatcaatcc
    24241 ggtatcagtc ttgactgtgg tctcgaataa tgacgaagcc gaaatcaaca tcctgaaagc
    24301 cagaaaaaag cttgaagaat tcgttaaaca ggcttctgca accgaaacga aggtgaatgt
    24361 catcaccacc atcgaccaca atccagcgag cggaatagca cgtattgcca gggaaataat
    24421 ggccgacatc atcgtactgg gatggcccag aaggacaggt ttgattggca aacttatcgg
    24481 ccagaaagtt gacagtattc tgagcaatac tgacaagagc acttttatct gctacatgaa
    24541 aaagcctttg atcctgcata agagaataat aattgccgtt cctccttttg ctgagcatga
    24601 aaggggattt gaattctggc tcttaaaaat tgcgaagctg gcacaggaat taagcgtccc
    24661 cattcaattc tactgtaacg aatccacaga gaaatcaatg agaagggttc tcaaggcagc
    24721 aaaacaatca gcttcggttt cttttaccag gttcattgac tgggaggact tcctgatcct
    24781 tgcacgttat atccgcgaag atgacctttt tgtactggtt tccgcccgaa aaggtgctgc
    24841 ctcctatatc tctgtgctcg agaaccttcc catgaaactg gagaagcact tctcacttaa
    24901 cagccggctc gtgatttatc cccagcaatt caatcaaacc tatgccactg acggctatga
    24961 agatataact gctgaccccc tgaataaagg aattgaaacc attcagaaaa taggaaaggg
    25021 aataagcagc atcttcaaaa aaggagatat ctagcgttcc atttcagaat ctgtcgtatt
    25081 ttccatcgat aaacacatcc cttacagctt ccaggtaccc gaatccgttc agcatgtcgg
    25141 gcagcaggta gctgccctcc acccattcgt tccaggcgtt gatggtgatc agcctggtct
    25201 ggccgggatg gctgtcggca tatcttttgg ccctggcaag cagggctgca aagctccggg
    25261 gtgtattgtg ataatgcacc acatcatgca tgccctttgc aggaaagcgc ggcgtgtcgt
    25321 cccagccaat ggaaacacac ggaaatacag ggatatcaag cagggaatcc atctgctccc
    25381 gtattctgat tgagtttgta ccgtaaacga tgtaatcctc atcaagccct cccatattgt
    25441 acattgcaac actgttaaac cccattgcct tcacattgcc ggcaaacttt gcggctgctg
    25501 cttctgacat gatcgaaccg cccccctggt tccactggat gtgcaatccc ggaaaaccgg
    25561 cctttttcac ttcatcgcga aaataatcga gagccctgcg tgcctcttca gtactgttgc
    25621 cgaagctttc aagcagcttg tcgacactga aaatggaaaa taccggttcg ccgttgatct
    25681 tgaaataatt cggacgcctg aaatactggt tgatcagcct gtctactatg atccggtaat
    25741 ttttccagtc aaccctggca ttccagagaa tgtcggtatt atccttgtaa cggtgtacgt
    25801 tccagtagtt gtatttgaca tcatggtttg cccacatgac atagaactgc attttttcat
    25861 tgttccgtgc accaaggaaa ccatcattaa gagcgctttc caggaacgga ccttcattgt
    25921 accagtacca gtcgtaaaca aatacattca cgccatgact ggttgccgct tcgatccatt
    25981 tttccacgac cttcggatca ttgtccgctt cgtaacccca aaagggctgc cggggctgat
    26041 aatgcccctc aaaacgcgga gtaccctttt ttataacttc ccattcaccg gtaccttcgg
    26101 gccagagcat ctccctcccc agcggatcat catgacagga gggccaaata taggcagcta
    26161 cgtaataacc gggtttttca gcccggtcat cctgcctgtc cgtctgtaca ccgggcctgc
    26221 aacctgaaag aataaggatt gttgcgaaaa tgacagttcg cgtaaaagag tttttcattt
    26281 ttttcaaatt aggtgactaa tgatcaatta cttgaattct gtattattgt tcaggatttg
    26341 ttaaaaacgg atccccgggt catacatccg gcaattttct tatggcatgc aggtttaaat
    26401 ttaagaaaat caaaaataac tctatgaaaa aacagtataa ttacacgaaa acagtaaata
    26461 tcaaatatca gtaaaaaaag cctttgacga aacaaatccc ggctaatata ttccataatt
    26521 ccccgggcgt tttattcagg gatttattat tagattcaaa tattaattct cctcagcctt
    26581 actgatggtt tattccatcc caggtcataa tgccccgtac ttaatgaaac acttcagaaa
    26641 atgtacttta tgcatatgaa tcccgcatga tacaaaatca caaacaagca ttaaaataaa
    26701 attcatgggg ttccatgtga accacttaat tttcagcagt ataccaattt attttcagat
    26761 gaaacccaca tgattgacca caatcacaaa cacgataaga atataatttt caaccgagct
    26821 tgcgagctcg acaaagtcaa tcatgtgcgg ttccctgccc gacgggtcag gcgggcatca
    26881 taccattaaa atcaaaatta tattatgatg aaaagaatac tattgatttt ttcagcggta
    26941 ataatgacaa cttcagcggc gggtcaggat cttgagatat ctgttgcacc gactgttaac
    27001 tttcctgtgt acaaggctct tgaaggcgga gcaggtcata atcccggaac agggttttca
    27061 atcactttcg gttatcttgc agcacaaaag cagtttattg cctggaccat cggcctgggc
    27121 tatcattttg tcaggataaa gaccgatccg atgttcaacg gagtatatga tcccttgccc
    27181 tacactgaat catgcagcct gatctcgttc agcattggtg ctgtcagcaa tctcagaaac
    27241 aatttcttca tagctttcgg accggccatg gatcttcagc tcaaccagaa gggccaaagg
    27301 acaatcgaaa ggcaaaccgg actgggtttt tcgctcagcg gggggaaaaa tatatcgctg
    27361 gacaacaatt tgggtctgag gatcagaccg gaaataagga gtcataacct gatcccgttc
    27421 cagggatggg attatccgtt aaggcttctc gtcgccggat tgaatatcgg gttgatcttt
    27481 taaaacagac ggctgcattg cgggctgaaa tccatgattc attcatgatc ggatcacatg
    27541 taccaaatca aactgcgaca acgggttatt tcaataccat ggtacagttt ttgcacggtt
    27601 aaaagcagga ttaaaccgct gatattatga acagaaatta cccgctgttt gcaactgatc
    27661 agctccgcat tgtcagaaac gggtttttcc cgccagaatt caacttaaat gcattcctga
    27721 acatcattgc cggtactttt ttggaaaaat cttccgaacc agttagttcc ctgtaattct
    27781 gaaaaaatga aaaaacaaaa cacaaccagc ctgatcaaaa tcttcggaac ggtcctgatc
    27841 atcactatct tcttcagctg ttccaccgta aacaggatct atacggacaa tgatctgaca
    27901 cgctcctcga agagatttga actgaaatat cttgtcaggg atcgtgtccg ccggtctccc
    27961 ctgcactttt tcaggcaaac aatcatcaag gaaatcggca gggataatga agttaattac
    28021 actgcctatg atgtgctgac attgtctggc acaagcttta cggtcgatga taaggtattc
    28081 ctgattattg ataacgttcc gttctcaatg aagatcgaca ggatggaatt tgaaaatgaa
    28141 agaagtcttt cggaagacac acaaaccttc ttaaattcag aatcggtgcc cataacggtg
    28201 atgaccggct attcggaaga taactccaaa attgcaagat tcagctacaa aataccccct
    28261 gaggccattg aaaggatcag gaattcaaat cagctgctta tacgttacta ttcgggccca
    28321 agcatgatga ccgtaaagcc ggggaagaaa agcttcagga aactgaaaca actgattgat
    28381 tctttctagg accggacact ccgctgatct cttttttcat gccggaatac cctgtgaccg
    28441 ggccggttcc cggcaaaaaa acgaaccgcc cggatacggc ggtcgttcca tcctccccta
    28501 aaccaccgta ccggggcccg cttgaaactg acagcttttc catcagaaaa tttcacacca
    28561 ggtattgact ttgatttata aagtagttat ctttactcat tattaaagaa gaatcattat
    28621 tatttaagaa tatttgtcta tgaaagccgt aaatacaggc agcaggcgga tcagggtgtc
    28681 ggtgtttggc attctgggag ctggcattcc gagaacagta tcaaacgctg gcaataatcc
    28741 tgctgccgga gagctgaccg gatactccgg gaattatcat ccgggtattt cggctggagc
    28801 cggttgccgg tttcctttcg gtttggcaga aagcggcttg tccggaaagg ctgcgacagt
    28861 taaaaacaac gagagggata ataacctcat agaatatcaa agtaatacat attgttcctt
    28921 ttcccgtacg attgaaggtt actttggcgg tgggccgggt gtatttcgtg aacaatattt
    28981 cctgaaaggt tgacggatat aacaacaggt gaaagtccgg taatcaacaa aagagaagaa
    29041 ctaaatttaa cacatgaaca tatggaaaag caaccgaaga aagccagcaa aagtcctgta
    29101 acaggggctg tcagtaatca cgtagccgga atggcaggta caaagaaccg cgactggtgg
    29161 ccaaatcagt taaagctgaa tattctccgc cagaactcat caaaatcaaa ccctatgggt
    29221 gaaggattta actatgcgga agaatttaag agcctggacc tgaaagctgt taaaaaggat
    29281 ttacatcgta taatgactga ttcccaggac tggtggcctg ccgattttgg tcattatggt
    29341 cctcttttta tccgtatggc atggcacagt gcgggcactt accgcattgg cgacggtcgc
    29401 ggcggggccg gctcggggca gcagcgtttt gccccgctga acagctggcc cgataatgtc
    29461 aatctcgaca aggcgcgccg tctgctttgg cctgtaaagc aaaaatacgg aaggaaaata
    29521 tcatgggccg atctgatgat actggccggc aatgtggccc ttgaatcgat gggattcagg
    29581 acctatgggt ttgccggtgg aagagaagat gtatgggagc cggaagaaga tgtatactgg
    29641 ggttctgaaa caaaatggct tgacaatgat aaacggaacc tcgatccgga agaaacagcc
    29701 aatcctcttg cggccattca gatgggactg atatacgtta atcccgaagg gcctggcgga
    29761 aatcccgatc cgctcgcggc agcaaaggat atccgcaaca cttttgcccg catggcaatg
    29821 aatgataagg aaacagttgc ccttattgcc ggcggacaca ccttcggcaa atgtcatgga
    29881 gcgggtgatg cctctcttgt cggacctgaa ccggaaggtg ccgggattga agaacagggc
    29941 ctcggatgga aaagcagtta cggcaaaggc aaaggggaag atacaatatc aagcggactg
    30001 gaggttacct ggacacctac gccgaccatg tggagttatt atttcttcga tattttgtac
    30061 aaatatgact gggaattaac caaaagcccg gcaggtgcat atcagtgggt ggcaaaaaca
    30121 acgaataagt caatccctga tgcccatgat ccatcaaaaa ggcatttacc ttccatgctg
    30181 acgactgacc tggccctgcg cttcgatccg gtttacggga aaatatcaaa gcgatttcat
    30241 gagaatcccc ttgagtttgc agaagctttt gcccgggcat ggtttaagct tacacacagg
    30301 gatatggggc cgcgttcacg ttacctgggg cctgaagtgc ctgctgaaga actgatatgg
    30361 caggatccga tccccgtggt taatcacccg ctgatcgata atgaagacat agcagctttg
    30421 aaatctgaag tgttaaaatc ggggcttact gtttcacggc tggtttcaac agcctgggct
    30481 tcagcttcta ccttccgcgg atcggataag cgcggcggag ccaacggcgc ccgtatccgc
    30541 cttgccccgc agaaagactg ggaggtaaat gatccgccca gcctgaaaaa gacgctgggc
    30601 atacttgaag aaatccggga ccggttcaat gatgcacaga ccggtggtaa aaaagtctcg
    30661 cttgcagatc ttattgtcct ggcaggatgt gcaggtgttg aaaaggcggc tgaagacgcc
    30721 ggctttaaaa taaatgttcc cttcagaccc ggacgcatgg atgcttctcc cgaacagact
    30781 gatgcagaat cattcaatgt attggagcca ttggctgatg gtttccgtaa ctatttaaaa
    30841 gccaggtata cggttagaac cgaagaactg ctgatcgata aggcacagct gctgacgctt
    30901 accgctccgg aaatgactgt acttgtcgga ggcatgaggg tgctcaatac aaattatgac
    30961 aattcgggac acggtgtttt caccaaccgg cccgaagttc tgaccaacga cttctttgtc
    31021 aatctgctcg acatgaatac tgtatggaca gccgatgatg atggcaggga aacattcaag
    31081 ggtcatgacc gtaagacagg caaactcaaa tggactgcca cccgtgccga tcttgtattt
    31141 ggctccagtt cagagctcag ggcaattgcc gaagtatacg ggagcgccga tgcaaaggaa
    31201 aaattcgtta atgactttgt tgctgcctgg tcgaaagtaa tggatctcga tcgcttcgac
    31261 cttggataat tgccggtaaa actgattatc cggagtaatc cggcgacttt ccggttcaat
    31321 gcttactgcc gggggcaggt gtaaggcttc agcccggccg cctgcctgcc gtaagtaccc
    31381 cctttgtcaa aagttaaaaa aaggtaaaaa agtgatgccg taaatcttat ccttccctgt
    31441 atttacgaat taaagtacga taaaacaggg tattacaccc gatatacgct gaaaaatcca
    31501 tttgaacacg ctttggaatc tcatagatta ttatgaattg aaatgttata ttggccttcc
    31561 ctgaattgaa gcagcggata aattgacagt cgctaattaa cacaacgcga aatggataaa
    31621 atcagcagga gaaaattcct tgagaaatcg ttactgatca acgggtcggt ggtactggga
    31681 tcggcatgga tctgcccttt tctggaaagt tttacattgc cgcaggcaat cctgtaccat
    31741 gttcagatac aggatgataa tccggccatt gcctggtcgc aggataaatg caaaggatgc
    31801 cgcgactgtc ttacggcatg tgccgaaaaa caattgatcc aggggtcata caaatcggtc
    31861 aggggacatc atgtatgcat tcattgcggc gcatgcatcc aggtctgccc ttacggggca
    31921 ttaacagaaa agtataatta ccgggaggtt ttcagtgccc tggaagatcc ttcaaagatt
    31981 gtgatagctt cgatcgcacc ttccgtcccg gcaggaattg gagaattttt cggactgccg
    32041 tcaggatcat atcttgacgg taacatagcc ggtgcctgca aaacgctcgg tttcgattat
    32101 gttcttaaca ataatttcag cgccgatctg acaatcatgg aagaggccca tgagctgcag
    32161 aggcgcatgg aagggaattc agccctgccc cagttcacaa gctgctgtcc ggcgtgggtc
    32221 aaatatgttg aaatgtacta tccgtcactg gtgaagcacc tgtcgaccac gcgaagtcct
    32281 ttctcaatgc agggggcatt ggtgaagagt tactttgcaa ggaagaagaa gattgatccg
    32341 gccagaattg ttcatgtagc gatagccccg tgtacggcga aaaaatatga gattggccgc
    32401 gaagaactca ccactgacgg gctgaggagt accgacattg aaatcaccac aaatgaactg
    32461 gcgatgatgc tcagggaaaa gaacattcat attgccggtc agaaaggcgc gtttgattcc
    32521 tcaatgggga aggcatcggg aagcgggatc atattcggaa attcgggagg cgtaatgagg
    32581 gcggctttgc gaacggccca ctataacctg acaggaaaga atcctgccgg cgactttctg
    32641 gagctgaaag aactgcaggg tctcaccgga ttaaaggaag ccactgtgaa gattggtgac
    32701 aggtcgctga atgtggcggt ggtttatgag atgagaaatg caaagccaat actcgagcag
    32761 gtgatggccg gaacctgcaa atatgatttt gtggaggtga tggcgtgcca gggcggatgc
    32821 atcggcgggg ccggacagcc ggcaaagacc atctccgacc ttgagaacag gatgaaagcc
    32881 ctggattcag ctgatgccgg cgctgctttc cggttcagcc atgaaaatcc tgaaatcagg
    32941 accatctaca ggaaattcct tggcaaaccc ggcggaaaag atgccgaacg gctgcttcat
    33001 acttattata ccgacaaatc ggaattgctg gttccggcaa aatcaagaaa accggcaaag
    33061 gtttcatagg gcgggatcgt attgtaatat attcattccc tgcctgagtc agcaacaggg
    33121 ttctgaaaca caaaagagcc cgctgaatta caggctcttt tgctgacccg tccgggctcc
    33181 ccgcctgcct gacagcgtca gcagtcgggc agggaaccag gccaggcgta aagaatcacg
    33241 ccggtggtgt gatttggcga aggagcctgc ctgccgcgct ggtccgggcc caggctttat
    33301 tcaacaacaa aatcccgaat aataaaaaag agcccgctga attacaggct cctttgttga
    33361 cccacctggg ctccccgcct gcctgacagc gtcagcagtc gggcagggaa ccaggccagg
    33421 cgtaaagaat cacgccggta gcgtgatttg tcgaaggaac ctgcctgccg cggcggtccg
    33481 ggcccaggct ttactcaaca acaaaatacc gaataataaa aaagagcctg ctgaattaca
    33541 gactctattg ttgacccacc tgggctcgaa ccaggactct gcggaaccaa aatccgtcgt
    33601 gttgccaatt acaccatggg tcaataccag aaacgatgct gcaaaattaa caaaaaattt
    33661 tattcaggta cccttgtgcc ggacagacag gatggtatga tttatctcag gaccataaaa
    33721 atctgaaaag aggaaaaaaa gcctctaaag ggcattaaga aattgcgtat ctgcaaattt
    33781 cttttttaaa ccggctgtct caacaatgaa aaaagggcac tcagtgagcg acctgaggat
    33841 cagtcccagt tcacacatgt catcatcacc ctgttcataa taccagagga tccgggcgta
    33901 tttcccaaca ttctctatcc ctgcaagctc cttgatgata ttgtaaatcc acttcgtaga
    33961 ggaggtattt atatattcaa atccgaactc gatctgtgtt tcttccctgc atatccttga
    34021 gtacctgctt atccattcat gaaccggacg gtatattgcc cccgggttct cagttattga
    34081 acgaccctcg aaaatgatcc tgccatgctc aagacaaatt gccggagtac gtttggttgc
    34141 ctcaacacag tatttgttca ttttcttcat catgcataaa acgtataacc cccgctttta
    34201 ttctattttt tccttaaaag gttttgttca tgatcggtca ttgtccgcag atggagaaaa
    34261 atcatgcagg agtataattt agatccggct tttgcgaatg gcattccgac cctctaaaac
    34321 agccggaagc tgatcccggg acagcatttc atttctgcat accccgggaa gatcccaaat
    34381 gactatatga ggcaaaactg cgtgccggaa atttgccatt aaccgtatcc ctgtccataa
    34441 atccgaacac tagtgcatcc gaatatgctg cggagcttaa tgatgataag aaaattcaaa
    34501 ggattcggga tgagccagcc tcgaaatggg atccctttaa aaatcaatga ctgctatcgg
    34561 gtcatacggc tgaataatgt cggttcaggt ttttcccaca gggaaggatg ctcatcctca
    34621 aatcttttca ggatggcttt cattattgtt tcccgcatga attccgacct gttttttatc
    34681 ctgtaacggg cacagtaaat attcaatgcc ctggcttccc tgctgttaag cataactgag
    34741 aacctgctgg tcctttttaa actgtcttct ttataaaggt tcctcttcaa aaaaacagaa
    34801 ataatcagct aatatagaaa aacagggctt tccggacttt ccggcttcaa atagttttga
    34861 acattgaata aaaagccagt ctttaactga ctggctttcc gagtgttaat atattaacaa
    34921 cttatccgct cctggaatgg gacacaaatc cgttgaacat caaaatatcc cgggcgccgg
    34981 ggaaccgcct taaaactaac attaaccatt cagtgtggat ggcattatta agccggaact
    35041 catattatcc tgaaccggct acaggagttg ttcctttgcg ggaaattcag ctgctaggct
    35101 tttccggcgc tgccaaggac attgacgatc ttatgcttgt acagctcctt cagcttctcg
    35161 cgtgcagggc caaggtaatt ccttggatca aactcaccgg gcttttctac aaataccttg
    35221 cggatggcag cagtcattgc caggcggccg tcgctgtcga tattgatctt gcagacggct
    35281 gatcgtgcgg ctctccgaag ctgctcttcc gacacacctg ccgcgttttc catcctgccg
    35341 ccgtatttat tgatttcttc aacaatatcc tggggtactg acgaagcacc atgaagaacg
    35401 atcggaaatc ccgggatcct tttttcacat tcttcaagta tatcgaagcg caggggaggg
    35461 ggaagttcgc cgggcttaac cttgaattta aatgccccgt gagaggttcc aatggaaatt
    35521 gcaagtgagt cgaccccggt cttttttaca aaatcctcaa cctcttcggg cctggtgtat
    35581 gaagagtgct cggcagaaac ctcatcctcg atgccggcaa gtactcccag ctctccctca
    35641 acggttacat cgtactggtg cgccaggtcg acaacctgtt tggtcagcct gacattttcc
    35701 tcgtaaggaa aatgtgaacc gtcaatcatt accgatgaaa aaccggtctc aatgcacgac
    35761 ttgcatgtct caaacgaatc tccatgatca agatggagaa caatggggat ctggtaacca
    35821 agttcctttg catattcaac agctcctttt gccagatatt gcaataatgt ctgattggca
    35881 tacttgcgtg cacccttcga aacctggagg attacgggtg actttgtctc aacgcatgcg
    35941 cttatgattg cctgaagctg ttcaagattg ttgaaattga atgccggaat cgcatacccg
    36001 cccttcatgg ctgctttgaa caattccctt gagtttacta atcctaattc tttgtaatga
    36061 agcataattc accgtttttt taatgaccgc caagatagtt cttttagctg ttacagccaa
    36121 aagatttgtt aaaaaatgat ccggctttcg cgggaatgca atattgaaac ctgacagaat
    36181 tcgcgggcaa tgcggaataa taattaagcc tctgaccatt aatattaaac tctaattgat
    36241 tatcttcgta accttatttt taagaatgtt ccgtcaggat aaaatgcagt tgcgatgcag
    36301 catctttaaa aaaagaggct cattatttaa gcattccgac tgattacttt atcaaatcat
    36361 tgaaaaatga aaacctttcc gttgattata atcatcttcc tgttgatttc tccgggaaaa
    36421 actcaagccc agcaggaacc atggtcgctt gaaaaatgta tcagctacgc cgttgatcat
    36481 aatattcaga tcaaacaaca ggtgcttcag accagggttc agcagaataa ttacaatcag
    36541 tcgaaactta atctgctccc ggtgattaac ggttcagcct cgcacgatta ttcattcggc
    36601 agggcgcttg accagaattc ctatgaattc tataaccaga ctgtcctgtc ggattatttc
    36661 tatgtcggag gcaggacaaa catcttcagc ggtttgcaga attacaacag tatacagaaa
    36721 agcagatatg aacttcttgc cggtcagcag gaccttcata agatcagcga caatgtttcc
    36781 ctcacagttg cactggctta tcttcagata cttctaaaca aggagcttgt tactgtcaat
    36841 gagaatcagc ttaacataac actgcagcag attgaaaaga ccaggaagct tgttgatgcc
    36901 ggcagtgtgg ccaggggaaa ccttcttcag atcgaggcac aggcggcaca ggaagaggtt
    36961 aatcttataa ctgtcagaaa tctgcttgaa ctatcctatc tcgacctcac ccagctcctg
    37021 gaacttgaat ctccggcagg ctttgagatt gaggttccca cgatagatat cggggccatt
    37081 tcccttattc ccggaagtgt ggaagaaata ttcagcctcg ccgcacaatc aaggcctgag
    37141 gtgaaaagtt cggagctcag gcttgccgca gcaaggatag atctgaaaat cgcaaggggt
    37201 tccaggatcc cttccgtttc actgagtcat tcattcggaa ccaggtattc gtatatttat
    37261 gatctgcccg gattacagtc gttcggtgat cagctcagga ataacaagag ttacgggctt
    37321 ggcgtagctg taaatattcc aatcctgaac ggctggcagg tgaacaggaa tatttccaac
    37381 acgaagcttg ccgttgaaaa cagcctctat tcactggaag gtacaaaaaa gcagctttac
    37441 aaggatattc agcaggctta tgcagatgca tcggccgcgc tgaagaagta taacgcaagt
    37501 cagaaggcag taaactcaat ggaagaatcg ttcaggtata ccgaacagaa attcaacatc
    37561 gggatggtaa ctccggttga ttacaatgct tccaagaccc agcttctcaa ggcccagtct
    37621 gatctggccc agtcaaaata tgaatttgtc tttaaaacca aggttcttga tttttacagg
    37681 ggtatacctc ttaacctcga caagtaaatg aattattagt cctatcacac atgagatcaa
    37741 acaataagat acttaagatt ctgctgccgg cagtggtggt actcctcatc tttgcagtta
    37801 tcggaaagaa gcagggatgg tttggccagg ctgccagggt aaaagtagcc gtcattaatg
    37861 cagagagaag ggttattgtt gaaacaatca cggcaaacgg gaagatccag cccgaaaagg
    37921 aagtcaagat caccccggat gtgtcgggag aaatagttga acttacagta agggaaggtg
    37981 accaggtggt gaaagggcag ctgctgctca ggattaagcc ggatgtttat atttcccaga
    38041 aagaccgctc actggctgca atcagctccg cacgcgcaag gctggcgcag gcggaagctc
    38101 agttcattca ggcagaactg actttcaaaa gaacggagca gctctactct gaacagacca
    38161 tttcaaaatc ggaatacgaa caggctgagg catcataccg ggttgcaaaa gctgaagtgg
    38221 atgcagcaag gttttccgtc gtgagcgcag aagcttcagt taaggaagcc gatgagaacc
    38281 tgataaagac ttcgatctat tcacccatga ccggcacggt ttcaatgctc ctggttgaac
    38341 ttggtgaaag ggtggccggg accggactga tggcgggaac tgaaatgctg agggttgcgg
    38401 atctttcaag gatggaggca agggttgagg tgaatgagaa tgatattccg ggcgtgaaga
    38461 tgggcgacac cgcctcaata gaggtggatg catatccgga atacaaattc aggggcgttg
    38521 tgacccagat agcaaattcc gcaaaaacga caggcgtctc tgccgaccag gttaccaatt
    38581 ttgatgttaa aatactggtt ctccctgagt cgtacagcaa tattgaggga agctctgata
    38641 cccatcccct caggcccggc atgtcgacaa ccgttgatat tcagacccgg atcagatcag
    38701 gcgttatagc cgttcccatt gaatcggtaa cgacacggac tgacacgacc aaacttattt
    38761 cctcaacatc cgataaagat atgcgtacgc tagtatttat taccgacggg atccgggccc
    38821 ttgcaagaga tgttaaaacc gggatccagg ataacagtta cattgagatt atctccgggg
    38881 tttctgaagg ggaaagagtc atttctgccc cgttcagtgc catcagtaaa aaattgtctg
    38941 acagctcatt tgttgaaatt gtaaagaagg aggatctctt ttcaataaaa tagatcaatt
    39001 tttcccgtaa agatcaagat gataatttgc ctcgaaactt ccggcacagt atgctcggtt
    39061 gctctgtgcg accgccaggg tgttgttgcc ctcagggaaa gtgctgaaag caaatctcat
    39121 gcatcgatgc ttacactttt tattgatgaa ctccttaccg atgcacgact gtcggtacaa
    39181 gagctggaat cagtcgcagt cagcaagggg ccgggatcgt atacgggact gaggatcggt
    39241 gtttcagttg ccaaaggcct ggcatatgcg gcatcaatac cacttattgc tgtggaaacc
    39301 acactctcaa tggtttacaa tatttcagac aatatcaggc aaaaatatgg cgttgataat
    39361 gaaacattgc tggcaccaat gatcgatgcc cggcggatgg aggtatatta tgcggtttac
    39421 aggtctgacg ggatgaaaat taaggaaaca acagccgagg ttgtcagtga aaagaccttc
    39481 aatgacattc cggagtcggt aaaaattctt gctttcggcg acggagccgc aaaatgcaga
    39541 caggttctga aaaggaaaaa cctggaattt gccggtgaat tcagaatatc ggcagcaggt
    39601 atgtataaac cggcatacga ctcctttgcg gcaggcagat ttgaagatgt ggcatatttc
    39661 gaaccatttt accttaagga ttttattgca accacgccca gggaagggtt cctgcccggg
    39721 aaacaaattg ccggttaaag gaaatttata acatcaatat tgatgaagca gctgaaagtc
    39781 ataatatttt gctttttgtc gtctctttgt gcaacagggc agaatctggc atacatgccg
    39841 ggagaaaaag ttgactacac aattcactac ggggttttgc aggggggcgt cgcatcactt
    39901 gaaatcagat ccgacacttt cggcggaaaa caggtctacc attcggtctt ccttggaaaa
    39961 accaccggac tggcagatgt tattttcagg gtgaaggata tctatgaaag ctacatcgac
    40021 ccggtaaccc agctgcctgt tttttccatc aggaatatcc aggaaggccg ttacagaaga
    40081 tataatgagg tcatatttga tcaccattca cgggctgact ctgccattct tacaagcaac
    40141 ctgacgggtg tccatattac cgaacaggga atacacgata ttatttcatg cttctactac
    40201 ttcaggagcc atcacctgcc ccgttatcag agcctgaaaa agggtgatat gataacgatc
    40261 atgacctggt tcaccgatga attgtacccg atccggctca gatacctcgg aactgaagaa
    40321 atcaggacta aactgggaag aataaaatgc ttcaagttca atcctgttac cgagaagggc
    40381 cggctgttca aaactgaaga tgacgtgtcc ttctggttca cggctgataa gaatttttta
    40441 ccggttaagg ttcgatttga tatatttgtg ggctccttta tagctgaaat agcagcctgc
    40501 gagggactga aatatcccct ggagataagg aaaaaagaat aacaaatact aaatttgaat
    40561 aataacttat ggcaagcaca gctgatttca ggaatggcct ggtaatagag ttcaatgatg
    40621 atctttacac aatcgtgcag ttccagcatg taaaacccgg gaaaggcccg gcattcgtga
    40681 gaaccaaact taaaagcatc cggaccggcc gggtaattga caatactttc tcagcaggaa
    40741 caaaggttaa cctggcacgg gtggaaagac gcccatacca gtacctgtac caggatgata
    40801 tgggatatta ttttatgcat atggaaacat ttgaacagat acacgtcccg gaagaactga
    40861 tcggcaacca tgaattcctt aaagaagggc agaatgtgga agttgtggtt cattccgata
    40921 cggaaactgt tctgagtgtt gaacttccgc agttcgtagt catggaagtg acttatactg
    40981 aacccggatt gcgcggggat actgccacaa ataccctgaa acctgcaaaa attgaaacag
    41041 gaagcactgt aatggttcct cttttcataa ataccggtga caaaatcaga attgatacca
    41101 ccaccaggtc atacatagag agggccaagg cctgacgggg atcccgcctg tccttcggca
    41161 cagacaggaa tgcggattgc ggattccgga aggcggattt atagcagttg agcatgacag
    41221 gattgcatga tgaatacaaa ttgtattctg actgttgtca gtaaaggtta aacggaaatt
    41281 gactctgaca aatgaaaaac aggcatgctt cttcagacag gttaaaaatg tccaggttta
    41341 aactggatgc actgctcgat ataacccttt cgataaatgc caatctgccc gcaggagatc
    41401 ttctggcaaa atacgaatcc attctgagga acaatcttca gattggaaaa attatgatat
    41461 tcaaatattc agaaacatgg gagtgtattc tgaacggcgg ctttccggaa aactaccgta
    41521 ccggtataaa cgtggaaaaa gatctcctta aatacgggaa aacggtattt cgtgatgaga
    41581 tacagggtat ggaaggcgtt gagatcatta tcccggtcta caataacaac gtaccccttg
    41641 cattcatact gatcggcgat attgatgaag aaggcagggg agtaagtccg gttataaaac
    41701 acctgaactt cgttcaaacc atttcaagta taatcctggt tgcaattgag aacatccggc
    41761 tcttcagtga gagccttcgc caggaagccc tgaaaaagga actggaactc gctgcaagaa
    41821 tgcagcagaa actgatcccc gacaacaacc tgatgcccgc cgacggcaaa ctggtggtta
    41881 cgggattcta ttaccctcat tatgaggttg gaggtgatta ctacgactgt attcagctgt
    41941 ccgatacaat tacagggttt tgtatagccg atgtgtcggg caaaggtatc tctgctgcaa
    42001 tactgatgtc gaatttccag gcagcactga gagctctctt tacaagtgac atcggacttg
    42061 tgatactgac ggaaaagtta aacaaactta cagttgccaa taccctgggc gatatgttca
    42121 ttactgcatt cctcgccaga tacgactcat catccggcat tctggagtat gtcaacgcgg
    42181 gtcacaaccc tcccgttctg tataatactg ctgacggagg tatcacatat ctgaattctc
    42241 agtgcgtggc aatcggaatg ttcgatgaga taccttatat acgtgaggat aagattattg
    42301 tccgggatta ctcaaagctg gtctgttata ccgacggctt gtccgacctg aaaggtgatg
    42361 acggaaaaga gatacttacc aatgagatag taaaaaatat ttccaacatg gaaccggtaa
    42421 gggacaatat catggatatg cttgatcagc ttgggatacc ggttggcaac cccggtcttt
    42481 ttgatgatgt ttcaattatg gctgccgatc tgtttgcacg ctgacaattg aacatctttc
    42541 agatcactct aagaatataa tagttttttt tacctttctg gacaagcagg tatttatgat
    42601 tgatcaatga attctccgtt attttttcct ccggatccag gatccggacc ttgttaatgc
    42661 ttaatcctcc tccctggacc atccgccgca gttctccctt cgattcaaac actcttgtat
    42721 gttccgtaca gagcgatgta atgctgactc cgctgcttat aagaccgcgg cttatctcat
    42781 aaacgggaac accctcaaaa actgaaagga atgtgctttc actcatccgc gcgagtgact
    42841 ctgtggtacc ctttccgaaa aggatctggg atgccccggc aacgctttca tattcttcgc
    42901 gggaatggac cataaccgtt atttcctcag caagcttttt ctgcagtatc ctctcatggg
    42961 gcgcggcctt gtgtctttct gtgagatctt caatttcatt tcttccaagc atggtaaaaa
    43021 tccttatata tcttgcagca tcttcgtcgg atacattcaa ccagaactgg taaaaatgat
    43081 agggtgatgt cttgtcagga tccagccaga cattgcccga ttcagtcttt ccgaatttcg
    43141 aaccgtccga tttggtaatc agcggacatg tgagtgcaaa ggcatcaccg ccggtcttcc
    43201 tcctgataag ttcggttccc gtcacaatat tgccccattg atcagagccc cccatctgaa
    43261 gtttgcatcc gagagtctta taaaggtgca ggaagtcggt cccctggaca agctgatagg
    43321 aaaattcagt gaatgacagg ccctcatccg tttcagaacc aagccgtttt ttcaccgatt
    43381 cctttgccat catataattg accgtgatat gcttgccaat gtcccttatg aatcccagga
    43441 acgaatattc tttcatccag tcgtaattgt tcaccatgat ggccctgttc tttcgctccg
    43501 gattgaagtc gagaaatctg gaaagctgat ttttgatgcc ggtttcattt ttcctcaggg
    43561 ttgtttcatc gaggagattc ctttcctccg accttcctga aggatctccg atcatcccgg
    43621 ttgctccccc taccagggcg acgggtacat gcccagcccg ctggaaatgc ataagcagca
    43681 tcacctgcac caggtgtcct atgtgcaatg agtcggccgt ggggtcaaaa ccaatgtaac
    43741 cggtggtaat ctccctgttg agcagttctt ccgttcccgg catgatatca tgcaacatgc
    43801 ccctccattt gagttcttcg acaaaattca tggtcctttc tttgtttttt cgaactgcaa
    43861 atatacgaaa gaggcttaaa tgggtattcc ttcgggtttc tttttgaacc tgtcaaaact
    43921 tttgtaaaag tcgccctctc cgataaccgg gaaaagtgca ggggcaaaat cggaaacggg
    43981 accgtaaaat ttcgactgct gcagtttctc cttgggaagg aaagggcgtc tggcatcaat
    44041 ggcattgagg atgacaaaga acacgctcag gatgagggcg gttttaaaaa gcccgaaagc
    44101 gatccctgca agcctgttca ggaagccaag ctcaatcgaa cctacaaacc tgtccactat
    44161 cacgcccaca aaatgaacaa gtattattat caggaccaat gttacaataa aggcaatgat
    44221 cccggtgtat ctgcctgtca tgtcaaaata ttcataaagc ttccctgcag tgaatgaaga
    44281 gaatctgata gccccccata tcccgaggat aagggctgcc agtgaagcaa gctctttaat
    44341 gaacccgtcg gtaaaaccac ggaaagcacc caggagcaga agcacaacta ttatcaggtc
    44401 gataaaattc atcagattga gattttaaaa catgaaatta ggaaaaagat cggggataat
    44461 acaaaaaatg ttttttactt ttggtttaca gacaattccg caataaattt ctgtaactta
    44521 atagcatacc cgattatgaa tgacccggtt aattcacgac gtgatttcat caggaagagc
    44581 gccgccctgg cggccggctt tacaataatc ccgagacatg cagtcagcgg actggggtac
    44641 aaagcaccca gcgacaagct gaatattgca ggtgtcggga tcggaggtaa agggcacccg
    44701 aatattgctg ccatgtcgtc agaaaatatt gttgccttat gtgatgtcga ctggaaatac
    44761 gccgataaat gtttcagggc ttttccggga gccaggagat accgtgactg gcgccggatg
    44821 tttgatgaac acggcagctc catcgatgca gttatggttg caacggctga ccatacgcat
    44881 gccgtaatat ctgcaactgc attgacgctc ggcaagcacg tctattgcca gaagcccctc
    44941 actcattcaa tctatgaatc acgtttgctt accaggcttg cagaaagata taagacggca
    45001 acccagatgg gcaatcaggg aaattcgggt gacggggtac ggcagctctg cgaatggatc
    45061 tggaatggtg agattggcga gataagggaa gttcacacat ggactgaccg tccgatatgg
    45121 ccgcagggga tagaaagacc caaaaaggta atgcctgttc caaagaccct agactgggat
    45181 ctttttatcg gacctgctcc gatgcgcccc tacaatgaaa tatacacccc gtggaactgg
    45241 cggggatggt gggattttgg caccggagct ttcggggaca tggcctgcca catcctcgat
    45301 ccggtttacc gggcactgaa actgggctat cccgaaaaag tcaggggaag ctcgaccgca
    45361 ttcaataccg aatcagcccc acaggccgaa accgtcgaat tcactttccc tgcccgcgac
    45421 aacctgccca ggctggcatt gccccctgtg aaagtttact ggtacgacgg gggattatat
    45481 ccggtgctgt cagatattct tcccgaaggt gagaacctta tggccgacgg gctgggtggt
    45541 tgtattttcg tcggatcaaa ggacaccctg atatgcggtt gcggcggttt caatccccgc
    45601 ctcgcctcgg gtcggatacc cgaggttaag ccatggctgc gccgcatacc tggcgctgtc
    45661 ggttatgtcg acggaccaca cgaacaggac tggataaggg cctgcaagga agcacccgaa
    45721 agcagggtcg aaacgtcctc caactttatg tatgccggcc ctttcaatga aatggtcctc
    45781 cttggagtgc tggcaatacg ccttcagtca cttaataaat ccctcagatg ggacggcgaa
    45841 aacatgagat ttacaaatat ctccggaaat gaaacactga aggtcattac gtccgatgag
    45901 ttcagaataa ttgacggtca tcccaacttt gatacaaagt atgccacttt caatgcttcc
    45961 gaggcagcaa atgaatttat tatgcatact taccgcgagg ggtggagcct tcctgatatg
    46021 ccggcatgat ctgcgtccag catttttact taccacggat tcattactga agcaggggag
    46081 ctggcagaaa ttattctttt tcctgcagaa catccggctt caccgtctgt atcccctgtt
    46141 gttccgggaa taccgttccg aagatccccg gattattgac ttttaccggg gggaccaaat
    46201 ccggcatctc cgtcatccag gacaagcacc cagtcccttt tgtcgggagg agtgaacctg
    46261 gtttcccttt tatcagtgaa ttctccggct tttttcgaca gcccgtttcc cgggttgaac
    46321 caccaggcga ttaccctttt tcccgaaatc cttttaagat caacaagaat accggctccc
    46381 gaagggatgt agatgacggc ggtttctcca ttatctgcca gtgccgccgc aacatatgag
    46441 ttttttgatc cgtagccttc cgttactgtg gagtgatcaa ggtcaggcac aagtttccac
    46501 caccttcttg agtcaacaag cttcttcatg ttcgaagcat cataggaagc ctgccagtcc
    46561 atggcatact gccatccttc ccctaatctc cacaggggaa gattgccaag ctggacccct
    46621 gcgatcgaac agagccatcc ccagtacatc tgcgatctta tctgcaggga atttgcattt
    46681 tgttcattct cataggtggt ttcgaaaaga atcaccggca tgggaggatc catgagatag
    46741 ttcctcagac attccgcgtg aacagtcgaa ttatcagaaa taaaggcata tgtgtaaaca
    46801 cagttgaaat cgagccagtc agcaccttcc caatgatcgc gcgatgagtt tgccggatgg
    46861 cagtgagcag tgaacaggtg aattttatca aattccctga ttccccttac tatttccgtt
    46921 tcaagcggcc gtgagaactg atcgggattc ctgtcaccat ccattatcca ggcaatattt
    46981 ggaaacctgg catatctttt accaagaaac tgtccgtatt tcctgcatct ctcaggacca
    47041 ttggcaagaa cttccctgta ccacccgtcc tgcaggcctt ctccgtcgta acccatatat
    47101 gcgggagtca tcattaccag gaacccgtga gccagtgcca tttcaagcac acggtcaaca
    47161 tgcctgaaat attcttcatc aggaaatgta aaatcaggga tgtttccttc aatatacctg
    47221 gtaaaaggat aattgcctgc tttattcctg ctggcttctg gataggccag tgtttcaccg
    47281 ttgaaccagt gttccagcag gtttacagtg attgtattaa atcccttctc tttcctgttt
    47341 tttaaataac aggcagtttc gttggaatca agtgcaacaa acagcgacca tggtgtgtcg
    47401 gcgttgataa agaaaggttt gcccgactga tcggtaaaaa aacgaccgtt actgcttatt
    47461 ttcaatggaa aatcactggt tgatgaatac gacagcagtg aaacaagcaa accggttaaa
    47521 agtaagatct ttttcatggc tgactggaac ttaatattat cattaacttc actagaattc
    47581 aaacataggc ataaatcagg aaaaatcccg gtaaagtgcc ttttcaatgg ttaatctcgc
    47641 ttcccgtttg taaattacct gaggccgaat cgtttttgcg ccaggcggcc aataacctca
    47701 acagtttcat caatgccgag aacggatgaa ttgatgcaga gatgataatg ttcagcggcc
    47761 ccccagtccc tgccgctgaa atagttgtaa taccttgccc tctccctgtc ggtcctttca
    47821 atatgtgtcc tggctttgtt ctcaggcaga ttctgtaaag cggcgacccg ttttatccgg
    47881 tcatcaatat tggcagtaat aaataaactg agtattcccg gattatcctt gaggacatag
    47941 tctgcacatc ttcccacgaa aatacatgat ttctgtgcgg caagcttacg gataacgtcg
    48001 ctctggatct tgaacaatgt ttcatcggtc agataataat tcatggtatg ggttgcgtcc
    48061 agaccccgga aaccggatat gctgccaaac aggctgaatc gtttcttttc atcggctttt
    48121 tcaaaatact tttcattcag cccgctctca cgcgatgcaa tacatataag ctctttgtca
    48181 taaaaggaaa acctgagaag ttccgacaat ttctgtccta tttcccttcc gccgctcccc
    48241 agctggcgtc cgatggttat aacatggttc gcgttcatta agtaatggca tttaggagaa
    48301 tcatacagat tttatgactg attttctgta ctgtacaata agcatataca acgaaataag
    48361 acttgacaaa aggtcagaca gtggcatact gtaccataca ccgtttacgc cgtagaacag
    48421 tggcagtatg atcaggcccg gcagcaggaa cagaacctgg cgggtgagtg acataaaaat
    48481 ggccttgccg ggcattccga tgctctggaa gaaatttacg gtcaccatct gaaacccgac
    48541 aatcgggaaa aaaaacatta cgatcctcag acccggcacc acaagatcta taagttcgct
    48601 gtcagtggta aacactgatg ccacaccatg cgggaaaagt tcacatatga gaaatccaac
    48661 caccataatt cccgatgcca ccattatggt cagtttcagg acccgaccca ccctgtcaag
    48721 aagaccggcg ccgaaattat atccggctat cggctgcatc ccctggttca gtcccatcac
    48781 tatcattgcg aacagcatgg caaccctgtt tattattcca tatgcaccta cggcaaggtc
    48841 acctccgaac ctgatgagcc tctggtttat gacaataact attatgcttc ctactgcatt
    48901 cataagaaag ggagccaggc caatcgacat tgaatctgta actatcttgc gtttcagcct
    48961 gaatgactcc ttcttcaggt aaatgaaatt cttacggttg ctgaaaaaac tgatctgcca
    49021 tgtaagcatt gaagcctgtg caagaacggt ggccagggca gctccacgga taccccatcc
    49081 aaaaacgaaa ataaacagcg gggccaggat aatatttatt atgacagtgg aaatggttga
    49141 atacatggct ttctccggat tgccggtggc cctcagcatg gcattcagac cgaaatacat
    49201 atgggttatc aggttgccga gcgagataat aaccataaaa tcatgtgcgt atggcagggt
    49261 gtcttcactg gctccaaaaa aatacaggat aggatcaagg aacagaagaa ttatcatcgt
    49321 gtagctcaat ccgatgatga tgttcagcac aaaaacattc cccaggatgc tgttggcgct
    49381 ttcataatcc ttctgaccta gcctgagcga cataagtgtt gaggcaccga tacctaccag
    49441 ggttccgaaa gcaatggcaa gattcatcag cggaaatgtg atggccagcc ccgagagggc
    49501 gagagccccc actccgtgac cgatgaagat gctgtcggtg atattgtaca gcgaagcggc
    49561 cgtcatggca atcaccgaag gaatcgcata ttgaagaaga aggttccata cgttcccggt
    49621 ccctaatgcc agaggcgatc tcatttgtgc cataatatgc aaatatactc catgttgctt
    49681 ttcctgaaca taaaaaacat gactccggtt gcaacagact gaaatgaaag tgaaaaatga
    49741 tatcagcccc tgaatggtcc ggtatctatg gctgccgggt acatcatttg ctgcatggtg
    49801 tggaacgata tttgcggcac aggaatgcct gtttatgata cggggaccgg tatttgacag
    49861 tatccgggcc ctgtgaaaga cattaatggt aaaaggatgt atctttgcac ctctccgtta
    49921 atgagtttct ttataaaggt gagaaataat taagaaaccc gtatgaatgg ccagaaacac
    49981 aggcacggat aaatataatc gctttgaggc atgcctggtt ccatgctccg ctggcagaca
    50041 ggtatctgat cttttaaaaa atgatgtact gatgaaatac agaaagggaa tatttaaatg
    50101 ggtctggact ttcagtctga caatgctggt catgtccggc tgcatgaagg atgtgcaaaa
    50161 acctgaagtt acaacaggta cagtttccgg ggttttgaca acatcggcat tgatagaagg
    50221 gaataccacc ggagacggag gcggccgtgt aatagcccgc ggggtctgtt tcagcaatta
    50281 cattgcggaa ccatatattc ttaaggataa cttgttttac cagggttcgg gcgccggatc
    50341 tttcaatgtt tatcttgaga acctgacgcc cggcacaacc tattatgcaa gggcatttgc
    50401 aacaaatgcc gaagggaccg gatacggaca ggttgttaca ttcaccaccc ggggttcgat
    50461 gacaggtgcg atcacattca atcccgacac tgtatacaat actgtaaccg acattacggg
    50521 taacgtgtac aagaccattc gcataggagg caaaacatgg atggcagaaa atctgagaac
    50581 ctcaagcctc agcgacggaa ctgcgatacc ggaagtaaaa gccggcgaag aatgggaaac
    50641 aacgcaggaa cccgcttact gctggtacct gaatgatgat aagtacaagg atatttacgg
    50701 cgccctttat aactggcaca cagtagcttc agacaaactg tgccccgagg gctggcatgt
    50761 ccccaaatat gatgagtgga tggcactttt cagccatttc ggcaataatt atgaagttgc
    50821 cgacaagctc agggaagccg gcgattcaca ttgggtaaag accggcccca atgtcaggaa
    50881 cgaatcggga ttcactgtcc tgccgggcgg ttggagaaat atcgacaata ttaatttcgg
    50941 cgaccttgga tacgcgggaa cattctgggc cgtcccgcaa acagggggtg atataaatac
    51001 cgtaatgttt ttcttcatgg gatcatggga aggcatattt aatgcacgaa acccgggaaa
    51061 cgcagggtta tcggtaaggt gtgttaaaaa ctgacctgcg cttcaagggt ttcctggtca
    51121 ggcatgaaga aaggaagaac agcctcaggg ataagacggg ccggatcaag tccctggagg
    51181 atccgcaggt tccggataaa acccgtttat gggctgaatt accggggcga tgaaggactt
    51241 taacatggtt tgatgcaatt ttagcatatt attttttaac cgtacaatat gctccgttat
    51301 tacaggaaac taacacggga actgatagaa aggtcattcc ctttgctgaa aggcaaaaag
    51361 attttaatag ggatggcgcc cttgaacttt tatgcctttt ccatctggct gccgccgttt
    51421 atgcgattga tggttttcag tacaaagacc aggaggtttg acaaaccggc aattaaaggt
    51481 cttgtagtcc atgaactgtg ccaccaggaa agatatatga gaatcggtgc attgaaatac
    51541 ctgggttttt cagttaaata tgtggtctca cggaaagcac gagctgccga agaaaaatca
    51601 accgacaggc tgacaatcga aaagggcttt ggatgggaac tgttcagact gactgagata
    51661 acccacaacg acaggaaaaa taaaaaaaca aacgatcttt acatgtcgcc cggggagatc
    51721 cggtcgtatt ctgaaaacct tggaaaatgg aatgccgcat catgaaccat catgacatcg
    51781 gctctattta ttaaaatggc tggaacaggt tacacgcaag gggttattgt gcgattcagg
    51841 cggcaggccg ggattttatc ctgatcagat attttctgat tccacccggc cctgaaaaat
    51901 cacttggtgt atttcccgaa atctaaactt caaagtaggt aatgttccgt attactatgt
    51961 taacgatcct gccgtcataa tactgggttt gtctgaccca gttgccggct tcatcgtatt
    52021 catactcaaa attgaatctg tattcctcgt tattcttcgg aaccgaaatg atgtattccg
    52081 ttacatcatt gtgcggattc ctgacgaacc tgctgatgct ttttatatcg ccgttcccgt
    52141 ccctttcagt ttgtgataca agctcgccgt tgacatattc atttgttacg ctgctactct
    52201 catttccttc cctgtcaatg gtcctggtta ctgaaatatc gtcacccgag taaatgtacc
    52261 tgtaggtaat gatattggta ttatccccat cataaatttt gagttcggtc atctttcctc
    52321 cgtccaggac aggcacctcc ctgcccgttt tcgtgttgcc ggagaattta tcaatcccga
    52381 tgcatctgcc ccttttattg tatatatatt ttgatacttc cgtaaccctg ccattgggat
    52441 ctatcatgga tgattcctcc aggtttccgc ttcggttata gacataggtt gactgattag
    52501 cttccagctt gtcagctgcc gggcaaacgc tcttcgcctc ggcattatga accgttttat
    52561 ccaccatccg gacgctgcct ttcagattgg caagttcaag atgtgtttta acaacggttt
    52621 gatcgttttg acatgagaag gcggttgctg ccagtaggca gattgctgca atttgtttca
    52681 tgatcagtta tttacaatga ataataagat acaggagccg gcaaaccgca ccattggcag
    52741 catatgactg ggatcaggat actttcatgt ctctctgcgt atggaacaaa ttaagccggg
    52801 gtccttagga tcagttgtca gttaaaatat gttaaacgct gaaaagtaga aaagaaaagc
    52861 ccagtcaata cttaagcaat tattaaaaca gacagcgata tgttaaaata aattaagaca
    52921 cctggtccga ctgccggccg gcctcgtatt atacgatccg ggagtcgatg ttttcgtacg
    52981 ggattctgtt actgccgcct tccggtattt tccctgaatt ctttaatttt aacaggtatt
    53041 tcaggatacc cttgtttttc atggaagtcc cgaaatccgg atagtcagcg gaagccgatt
    53101 gctgacccgc agtgcccgtc acacgggaaa attatattta cctggtatcg atctgaattt
    53161 atgaaaatac attttgaaca tcacacgcag aaagtattgc ctttttcaag gtttcttcta
    53221 aggttgggca aatactttgt tttctcatcg gtcctgatcg cattttcact ggtgctggga
    53281 atggcaggat atcattttgc aggcaggtta aacctgctcg acagtttcca tatggcgtgc
    53341 atgattctta ccgggatggg acccgtcgtt gagctgactt cgcccggggc aaagatattt
    53401 tcatcattgt atgcactgta cagcggaata gcatttctga gcattactgc cgtgttcctc
    53461 tcacccataa tccaccggat attgcacatc cttcatgttg agagtgagaa cagggcaaga
    53521 tgaatacttg gtccgggaat ccctggcgac atggtcggta acagtccggt tggtgatatc
    53581 gcgctttaag ctccgggagg tccttttccc caatgaatgg ctggtactgg cagaaactag
    53641 ccggctgccc gtgtgactgt ccatattatt ggacagcaca ctgaacagcc ggacagttca
    53701 ttgatagtct gccctgcctg aactcagatg ccggcccggt tttaccagcc cggattctgt
    53761 ttcaggttag ggttgagggt aatttcattt gacggaatgg gagccaggta atcccgcgaa
    53821 ggattgaatt tgtatccatt gggcaggaat gcctggtagg gatccacaaa accgtcctca
    53881 tccacaaaca gattgacacc tattgtaatt gtcgggttcc cgttggcatc cttataggct
    53941 ccttccaggt cagaaccgat gtatttgact cccctgggcc ttttattcac gatgagcccg
    54001 tcagccttcc agcgggcaat atcatcccat cttgttcctt caaaggccat ttcaaccctt
    54061 ctctcccttc ttacttcatt tataaccggt gacaggtcgg gaaaatccca gcggggatca
    54121 gtggcaatat cgttgatatc caggtgtggc atgtttaccc tgtcgcgaag gaggtttaca
    54181 gatatatctg catctgcctg ggtgagtgtc ccaagttctg cctttgcttc agcgtagatc
    54241 agcagggctt ctgcatagcg gaatatgatt gaccctttgg ttccgatatt ttctgcagtt
    54301 tgctgggtat attcattatt gataccctta cgcagactgt aaccggttgt gctcctgaat
    54361 ggtccgttct gatcaatcgg aggcagtccg aactggatga atgccccccc tggaccgttt
    54421 tgtgtctgat agtccccttt tttgtaaatt gtttgtgaaa gccgcggatc cctatcactg
    54481 aattcttcca ggagcgaacc atagccatga aagtcagggc ttacagaaat cggtttgccg
    54541 tcggtgcaca gataactgtc aatcagccct tttgtaactc ctgtattgcc tccgccccct
    54601 gaaaggcttc gtgaaacatt atttacgatt ttgagcgaca ggtcaaaatg tttccagagc
    54661 attacctcgg ggttcccgga gtaatcaagc tggttgaaaa gggaacggta ttcatccccg
    54721 gcaggccctt tataaatgct gtagatattc atatccatca gctgtctggc ggttgcttca
    54781 gctttctgga tcaatgctga accatctgaa ccctgaaccc cgaaaggatc attctgatga
    54841 tacttttccc aggttccctc gtagaggcat atgcgtgcct tcagaagcat tgccacctgt
    54901 ttgtttatcc tgaacgggtt ggcttcctca acactcttca ggtaactgat cgcgacatca
    54961 agatcggcca ggatcgaatc cacaacgatg tcgcgcctgg tgcggggccc catcagttcc
    55021 ggtgaattgg tggtcagagg cttggacacc catggcacat ccccgaagat cttaaccttg
    55081 ttgaaatata aataggccct gaagaacctt acctctccga tgtaatgatt tatttcactt
    55141 tctgctccct gggccttgtt gcagtgctca agaaaataat tggcctgcct gatgtctgac
    55201 cattcccagt cgcctcccga aggcggaact gtccgtactc ccgctagccg cggatcaaat
    55261 gcggaaggcg ccatattgtc actgttctga tcgatcatta tcgtgaagaa atagcctgca
    55321 tcggcagtaa gaagaggata aaacctgttg gcatatagct ccaggtcttc ggcagaactg
    55381 aaaaatgatt cagatgtgat ctgatccagc ggatatttgt tgagcaaatc ctcactgcat
    55441 gagctgaata gtaaatatcc tgccagtatg atcagtaatg ctcctctgcc ggctgatatt
    55501 ggtgaatata atttcatgat tcaattgatt taaaaggtta cattaacacc tgatgtgacg
    55561 gtcatttgaa tgggatatga tttccctgat ccgtaatagc cgaaagtgcc ctccggatca
    55621 aaattcttca tcagcctggt aaaggttaaa aggttttcac cggcaatgaa tatcctcagg
    55681 tttttgataa aggccttttg caccagcgat gcgggcaggg tgtaacctat gcttatggat
    55741 ttcagtcgca ggtatgctgc gttctgaaga tatcttgtct gtacctgctg gtttttagat
    55801 ccttttccca ggtatggggt ggggaaataa ccgtcgggat tttccggact ccagtaatcc
    55861 atgtgttcct caaaggcggt agcgttccac aagccccctg cagcccccca gaattgctgg
    55921 ccggagagcc acagatccct tttgcctatc ccctgcagca gcatattcag atcgatgcct
    55981 ttccatgagc cgtttaagag aaggctgtat gagtatctag gtgtggaatt cccgataatc
    56041 atcagatcgc cgtgatcatc cacggtgttt ttcccgatgt cgatcttgtt gtcggaattt
    56101 aagtcggtat agtgtacgtc acctgcacgc cagggctcct tcgaaaggta cgactgatca
    56161 acttcctggg cctcctggtc cgactggaac aatccggctg ttttataacc ccatatttcg
    56221 ccaatgtact tacccttgta aaaaggagcg ctgagaatcc cctgggggtt ataataatcc
    56281 agaaccttgc ttatattgtc ggcaagcatg aattcgacga aacgcaccag tttctcccgc
    56341 tccccttgct cc
//
LOCUS       DAOOYG010000004        55429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_483_length_55429_cov_7.794579, whole genome shotgun sequence.
ACCESSION   DAOOYG010000004 DAOOYG010000000
VERSION     DAOOYG010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 55429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_483_length_55429_cov_7.794579"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..1924)
                     /locus_tag="PK288_00785"
     CDS             complement(377..1924)
                     /locus_tag="PK288_00785"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPM17479.1"
                     /translation="MRRRKFVKSVGVLTAATYVYPGKAIEEASGLFHHGKLLEAPSIF
                     FEQDGSNSAGPEDGPNQPMGRALGGNPGRVVWAWNPGATNPDCKNVIENNNWYFNPVN
                     FDQKIIDSMFSESILKLTGESKLNKAWDQLFRYHNLKKHNKDRGYTPGEKIYIKINQG
                     TSDWLLSREDKANGYFVPSYPLKPGARSRGYGAMETGPYIVLELLRELVNECGINQSD
                     ISIGDPHKHIFGHNYSYWHKEFPGIRYTDKYSDAFNRTVVKETEKVLLFYSDKTMTDR
                     LFDVIEDADYMINLANFKPHGSAGISLTTKNHFGSIAGRASRLHYSTVAPSWETSPRQ
                     VGNPNNRGYRKYRAMVDLMGSKYLGRNTMLFIVDGMFGGGSDETKGPVKYFMPPFNGH
                     WSSSIFMSQDQVALESVCFDFLRTEWDGNNKHDPANAVWEWVPNMPGVDDHLHQAADP
                     KNWPDGLIYDPDNSGIPLTSLGVHEHWNNPVDMQYSGNLGKQNGITLVSVGDYNVKIE
                     KRQKSRT"
     gene            2345..3205
                     /locus_tag="PK288_00790"
     CDS             2345..3205
                     /locus_tag="PK288_00790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPM17480.1"
                     /translation="MEKEINALYRAYKDGKDSRRSFLRKLALITGSSAAAVALFPVLE
                     ENDLKAAGTTGRASPELSIGFVKYPAETGDMRAYQARPKKGKKLPGVIVIHENRGLVP
                     HIQDVTRRMAGEGFLALAPDALSPLGGTPDDTSNVGEMFRKLDSEKTTKNFVAAVRYL
                     KTNPLCSGKVGCTGFCWGGAMTNQVAVNAPDLDAAVPYYGRQPAPEDVEKIRAPIMAH
                     YAGNDPGINAGIPAFEEALKKYGKEYQIFMYDGAMHAFNNDSNPERYNEKAAKLAWER
                     TVGFFKEKLK"
     gene            complement(3238..4383)
                     /locus_tag="PK288_00795"
     CDS             complement(3238..4383)
                     /locus_tag="PK288_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HPM17481.1"
                     /translation="MTETQNYLPEFQIFVKPAGARCNLRCRYCYYLDKLSLYHDGSDL
                     LMTDEILGKYIIQHIEATEGPVVFFSWHGGEPLLAGIDFYRKALEFQRRHLPRGKSAI
                     NGIQTNSTLIDRDWTWLFRMENFIVGVSMDGPGELHNHFRRGPSGRTSFSETLRGFEM
                     LMQEGVSAEILCVVNSFNAEYPLAVYDFFRNIGAENITFLPLVEPRPDLPGGVSPASV
                     SADAFGSFLIAIFDEWVEKDIGRIKIQIFEEAARTAFNQEHTLCIFRENCGAVPVIEA
                     NGDFYSCDHFVNSENLVGNIGNNSVAGLLYSDKQKAFGLAKSCSLPYYCKICDVRTMC
                     NGECPKNRFITAPDGEPGLNYLCSGYKAFFRHCLPFVRAVAEGWNRK"
     gene            complement(4556..6130)
                     /locus_tag="PK288_00800"
     CDS             complement(4556..6130)
                     /locus_tag="PK288_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HPM17482.1"
                     /translation="MKFPKVLIVILILIFSWSLCAKAQDKNKKHPSVRIPENRVWYVT
                     DFGAKGDGLTLNTSAIQKAIDACYRAGGGMVRFTRGTFISGTLFLKDNVTVHMDADAV
                     LMGSRNRQDYQPIAGLLTVEGKPSFGAEGSFLIYAENAKHISIEGRGEINGNGDTFWE
                     EEMFTKWVRKPREWRPRSLICFVNCKFIIIRDIQLNNSPCYTLWPLGCDDINIEGITI
                     RNPLDGPNTDGIDIDCCRRVSISNCNIEGGDDAIAIKSDSGRLGHEERPCENIVVTNC
                     ILSSPPACAIRVGYEGDSPIRNATFSNLAMYRSNHGIDIISILPDRGDDFPIIRGTRV
                     ENIQFDNIVMQDVTQPIYLWMGNDKKDIESSCFMRNIRISNLIATSGGTSFIGSTMEK
                     HIENINLSNINIIPSGKVQKDQPLEVTVWNSESSCALYVANVKGLNVDGFSVDFSNAG
                     GFWTHAVYCNNSRDVVLSNILTNSLGTVKLISQIGAVNSSLRVRNCNPEDGIESIYSD
                     DNSRVITTGCNEEKTN"
     gene            complement(6854..7408)
                     /locus_tag="PK288_00805"
     CDS             complement(6854..7408)
                     /locus_tag="PK288_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17483.1"
                     /translation="MYTKTIKREVRIFSEKNTNAGKSVVPKENIEEINCQAIDNLIAE
                     GGSNFIRYLGWLNMLNQKNILLLSPKHHYFFDQTELETPTILILLKRLNQMENLNGFL
                     DGISKTVGPGTSLMGHFFASGSKTDKPEVLSSIYNKLIDILDPGILKRFDSESVADLI
                     ESYGFEVTDLTMIKNSVYFLAVKK"
     gene            complement(7555..8175)
                     /locus_tag="PK288_00810"
     CDS             complement(7555..8175)
                     /locus_tag="PK288_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17484.1"
                     /translation="MKYILASAFLFAAICAYSQDRIKMKSGETVLAKVLEVSRDEVKY
                     RRHSNPDGPVYVLSKQEISEITYQNGETEVFNSVKAPENNNRNDNPPVDPAIVDMFYS
                     MTRRNNRVYITSENDNAVVHATNAINRWGYWTVTEKKYNADFILNFNINFSGAEAYGT
                     ADFINPRNGRIIRSTNQFRVAPTWDLNVKRGLVNVIVDDGIKPMFR"
     gene            complement(8391..8909)
                     /locus_tag="PK288_00815"
     CDS             complement(8391..8909)
                     /locus_tag="PK288_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPM17485.1"
                     /translation="MDYTELIITRRSIRSYDPDRPVPEAVLRKILEAGRLAPSAHNNQ
                     PWKFYLVSSPLLLEKIKMCYKREWIRDAPHILISAGLRREAWTRDYDGYNSMETDVAI
                     AMTQIILAAANEGVGTCWVMAYNPFELKEALDIDDNHQLLAITPLGYPKAGYQVPVEK
                     GRKLLDEIVEFL"
     gene            9202..9852
                     /locus_tag="PK288_00820"
     CDS             9202..9852
                     /locus_tag="PK288_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17486.1"
                     /translation="MKKTVLLFISILLLGTTTVTNAQKDRALSNGFSIHLIGGLPSGN
                     FGLEKDAHIDKKFKLNPLMGFQIGNRWYFTPGERFRAGLMVNWFDLTFAFKTTSTDSF
                     DWSRYVADVAVLELGPVATFKIANNMAVDGYYNLRPSGFATMFVTNLFGEDESDTYSG
                     GGISHAIGGAFRWNILSVGAEYVLGGIKCKNIDPESDDTSSKLLVNSVRIMVGVKF"
     gene            complement(9968..11125)
                     /locus_tag="PK288_00825"
     CDS             complement(9968..11125)
                     /locus_tag="PK288_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPM17487.1"
                     /translation="MTRKERLTATLNGLTVDRPAVSFHEIGGFLIDPDDPDEYNIYNA
                     PSWRPLLDLAEEHTDLIRMVSPVRAQSHLSWDGSTNTEKRRFYVKEERWEGCNSRFAR
                     ITYSINGRSLTSISRRDREIDTVWTIEPLIKTIDDVQIFLDLPDVIFEENIDAEHIRQ
                     EESKLGDRGIIMIDTEDPVCAVASMFRLEDFTLFAMTEKKLCHALMEKHSRYIWERVE
                     KVSGYFPGRLWRIYGPEFITEPFLPPELFEEYVVRHTGPLVEKIRRNDGFVRIHSHGR
                     VRNIMEYIAGMGADAVDPLEPPPQGDVLLKEVREKYGRELVLFGNIEIADIENMSPEK
                     FRETVKRSLSDGTSGEGRGFVLMPTASPYGREISGRTLENYQIMIEEAEKV"
     gene            11404..12240
                     /locus_tag="PK288_00830"
     CDS             11404..12240
                     /locus_tag="PK288_00830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM17488.1"
                     /translation="MRMIKIPILFFLANLFCAVISGQEYLKASDLDKPDFQWPEGKKM
                     ALSLTFDDARLSQVDKGIPLLDRYDVKATFYVSISNMIQRLDGWKQAIRNGHDIGNHS
                     LLHPCTINYGWPQEAALENYTLQRMTKELDSASVMINNILGIRPHSFAFPCGQTFVGR
                     GVNLKSYVPLVASMFESGRLWLSEGPNDPVFCDMAQLTGMELDGKSFGQIKSLIESAS
                     IKGQWLILAGHEMDEQGRQTSLLETIEAICKYASDPANGIWIDNVHNIAAYIKSKRGH
                     DD"
     gene            12544..13233
                     /locus_tag="PK288_00835"
     CDS             12544..13233
                     /locus_tag="PK288_00835"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPM17489.1"
                     /translation="MSKHIGIIACSYEGAALCYRTICNEGSDYLGRHSHPEVSLHNHP
                     LSEYMKYIEKGDWEGVASLMISSARKLAGAGAGILVCPDNTIHQAYEIVRKEIPVPFL
                     HIAEEVAGEAFRNFRFKTGILGTKYLMEGPVYPEKLSVLGIKSEIPSGSRRDRINSII
                     FNELVYGIIEPGSKEYFISVINEFRQSGCDSVILGCTEIPLIVVPEESPLPVLDSTRI
                     LARAALRKAIE"
     gene            13541..16627
                     /locus_tag="PK288_00840"
     CDS             13541..16627
                     /locus_tag="PK288_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM17490.1"
                     /translation="MLRGQFSQRILFILILAFFQIPLFAQQITVRGRVVNSDGTPIPG
                     VTVMEMGTAKGVITDADGIYSLTVSPAASLVFSFIGYNKEEIPVDGRTNIDVTLTESI
                     TNLDEIIVIGYGTQRKSDLTGAVASVKIDELKGRSIINVEQMLQGTVAGVNTVSSSGL
                     PGGNVKINIRGVGTFYNTNPLYVVDGIQTRDISLINPNDIESMEVLKDASTTAIYGSS
                     GANGVILITTKRGAEGRPSVEFNSKLGLAHRGKKIAVMNAPDYVDFVLDLKGNDVNQS
                     FLDNIDWRRVNRTDWQDELFRPAFQQEYNFAVSGGSKDARYSIGAGYVDQDGIIITHN
                     YKRYSLRANTEFSPGERFKVGENFTVSFSNASEAIEGQGNGNMLLCALRIPPYLPVLD
                     PDNTGGYSRVTSARDHNDAYNPIAELRLRDNKTNSLRILGNLFGELEILRNLRYRTSI
                     AVDLERNDVNTYTKARANAAFEFPSILDETYSYGNSYIYENVLSYANKFGLNDFLVLA
                     GNTLSKSSGRYYSLTATDFTNDQIRIIPVGTGKINNGAVGANQSAKLGYFVRLNYSYA
                     DKYLFQANFRADASYNFAPENRWGYFPAFSLGWKLNEENFIKDNLTFVDQLKLRAGWG
                     QSGSDLIPSYGFSSSISSFNPDYIFGSVPVTGAAIVSLWNPLMKWETSTTSNIGIDAA
                     LFKSRLQLTIDYFIKNTRDILIEYPMPSSTGIGLSGGSEGSAWRNAASVVNKGLEMTV
                     NYTKTSTSGFTYNFGANATFLKNKVTDLGDGLPIMAGSFAGNAMTRTEKGHPIGSFYG
                     FKMDKVYSTQAEVDADNERARSLHGPDAMYQENAQAGDIRFVDVNNDGLITDADKTFI
                     GCPIPKLVYGFNLGASYRGWNLSANFSGVSGNQIFDSNQYWLEGMVIPFNASIEIKNR
                     WKKEGDISRIPRAVSPDANQNTRVSDRWIHNGNYLRLKVLSLGYQVPALLLQKLTSNT
                     ASNLRIYVAAENLLTITGYRGFDPEIGEMGDNLVQGVDWGQYPQSKLYMFGLEVKF"
     gene            16640..18169
                     /locus_tag="PK288_00845"
     CDS             16640..18169
                     /locus_tag="PK288_00845"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM17491.1"
                     /translation="MKRIIYLFFGILFFAGCSDDLLNLENPNQYSADTYFKNLEECQR
                     GINAIYGGFYHDGLYAREYYFIFDCLANEAEQNSPMEAPTTDWPNYTHHPGNSELQGL
                     WRSLYRIIFRANVAIENIGKWEPVKESEAQRKETMIGEAYFLKGWAYFELVNIWGRVP
                     IRTFENSTEAKVPRSNTEDVWKTVEDDLKEAIDRLPVSWDNDNIGRATKGAATALLGK
                     AYLYEKKYAAAHAEFAKLASAPYTYDLHPDYQENFLEAGDNNEESVFEVQFRGGATGD
                     AVWYMFGGQESWGIGGLHTGRATEYGFNDWENCHVSNAAVAAFTYKNESGTDYIDPRG
                     AMLFYGDAASGGDTDYAGGEFPYEAKGYMWKKYQTYEENEYEGDPNSGINAKVIRYAD
                     VLLMRAEALIMDNRIGEALPLINKVRARVNAFEYTDLGNQSEAMQKIKLERRLELCGE
                     QVRWFDLIRWGEAMEVINAEKFAAKGVRPFLPKHVLFPIPQEEKDANSLVADDITGDW
                     N"
     gene            complement(18329..19429)
                     /locus_tag="PK288_00850"
     CDS             complement(18329..19429)
                     /locus_tag="PK288_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPM17492.1"
                     /translation="MREKLLSPALLAVLLLVSGCSVQKIDKTRAAETGVKNVILFIGD
                     GMGTSHVYAAMSTSKKPLVLESFPFTGFSKTYSYNGYVTDSGAGGTAIACGVKTNNGM
                     IGTGPDSAAVTSITEIAHRNGLATGVLSTSAVTHATPASFVAHSSGRGNYEDIAKDFL
                     NGTVNVFIGGGEDHFRNRNDNDDLTLKLRQQGYDVVYSLADLKKSESKKIAGLLAKEH
                     MPSVAEGRKGILPEMVKKAIDVLSRDEAGFFLMVEGSMIDWGAHEKNLEYAVSELIDM
                     DEAIGVAKKFAEKNGETLIVVTADHETGGLSLTGGSIPGKLVKGEFIESGSHTGVMVP
                     IFSFGPGAARFSGIHENTFFLNEFVDLLKLKK"
     gene            complement(19484..20953)
                     /locus_tag="PK288_00855"
     CDS             complement(19484..20953)
                     /locus_tag="PK288_00855"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPM17493.1"
                     /translation="MTRKSFFCATCLLLLISSCNNKKPAKDEILSGEYWKTQAMRDII
                     PAWTRYSQNERSGTFHTNLDSLWKPFGSTDIYPSMISRHLFSYSAAYLLSGREEDLEI
                     AGKTAAWLIEKAWDREQGGWYDALDEEGNPVELTKTTFVQVYVITGLAMYYFATRDSV
                     ILSYIERSNDLLEKRVWDMDGGGGYFNIMNRDWSILDSNKSFSSQVTPVSGYLFYLYL
                     ATRDQKYLDQTFKILDVVSKRMVDTEYGWVLEDFDSKWNYLSSRNDDSEINTGHNIET
                     AWMMLRYWFLTPDEGNKNAGLKIADKILRSGVFNENNVWLATAGRTEPSMPGSDTYWW
                     IQAYGNMISLCLYKATGDAKYIDYFIRGARLWDSAFVDRKHGDTFFRIDSAGNVLDRT
                     KAGRFKSSYHNMEHCLLNYLYLNLWVNCEPVVLHFMVSSPVAGEKLYPVPVEDMGARI
                     VKVTSPDRGDSSVEFSGQAVFLPADANLRLEVELRGCGK"
     gene            21142..22227
                     /locus_tag="PK288_00860"
     CDS             21142..22227
                     /locus_tag="PK288_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPM17494.1"
                     /translation="MTHDSHYDLVRKLRPARIILPVIIGVVVVGWLMVREIDTRALSE
                     LNFTWKSAFWIGVAWCCMIGRDLGYMIRIRILAEKDISWLQAFRIIMLWEFASAISPS
                     TVGGTAVAVVFVHKEGISVGRSTSIVLATSFLDELYFVIMFPVILLVVGNDILFTTSL
                     QGTGITLLNNLVIVAVIGYALILAWVIFIGYGLFIDPVKIKKTITYLFSLPLLNRWKG
                     SAEKAGNDIIDSSHELRRKSFGFWIKALTATFLSWTSRYWVINAILVAFFTINDHLLI
                     FARQLVTWIMMIISPTPGGSGFAEVILGRYISDAIPGDPVTTGSLALAIAIIWRIISY
                     YPYLIIGTSILPAWINRKFVKPGPGGN"
     gene            22345..23256
                     /locus_tag="PK288_00865"
     CDS             22345..23256
                     /locus_tag="PK288_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17495.1"
                     /translation="MKVKVLLILLIGLLLHYSCSNSPQKPAGSPAAADSPEHYGSGYS
                     SPESENDFPVNYFSPVEIADLFQKIGVPFSTEYLASPIDPRTLTTSFDKAVTLGILCA
                     DLGYMNMYEMTGSFPDLIASIKTLAEDLGVSQFFDFDAITRLSFNKSNMDSLLFLSLT
                     SYSQADHYLRENDLGHLSVLMMTGVWLEGQYLTVKVFEKYSDPKLKDRIGEQKLFLNH
                     LIETIKNYCGKDTQFERLCSDLRKLNDSYTDVRISYTRGEPVATKKGKGLAIQQTETS
                     NVEMSQEQLARIMEASAGLRNNLIKKN"
     gene            23361..23798
                     /locus_tag="PK288_00870"
     CDS             23361..23798
                     /locus_tag="PK288_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17496.1"
                     /translation="MKKYCIGLILFLASVTCMYGQKNPDKFISNCIANTGPDSKYLKD
                     FRIQLGQSTTSNEFRFKAKIYLWKDTKYRFTMCTAENSKGQLIMNIRDELNKLILESS
                     DSKTGTIYPFIDFNCTRSGLYQICYDFSGMLSGSGAGIISILE"
     gene            complement(23803..24798)
                     /locus_tag="PK288_00875"
     CDS             complement(23803..24798)
                     /locus_tag="PK288_00875"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HPM17497.1"
                     /translation="MLNKFRGVTPDVIILIVLVAILVWMGAFLHPVPQPAPDFSLRPM
                     PLYNLLLSVIGFNPTVNVVAAFLLLLLTAFLLVNFNTSVFFIGERTFLPALIYILFSG
                     LFPEQQQMNPVLPAAVFFIMALRKIIDSYKIQGTAYILYDAGFLMSIGSLFYANFIWF
                     VILLFIGIAQLRPVNLKEIVISMLGLATPWFLVSGFYYVTGKNLTEFSDLVRYNLFEK
                     ESIYVFSRVAITVLIINGMILIISFADLISVLNTKKIRSRKTFILLTWAFLTAIAVMN
                     FSRTVSVEISWLAAIPACYVMSHYFVFAGKKRTVPVIYFLILFIETALIQVLHFI"
     gene            complement(24788..26071)
                     /gene="purD"
                     /locus_tag="PK288_00880"
     CDS             complement(24788..26071)
                     /gene="purD"
                     /locus_tag="PK288_00880"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPM17498.1"
                     /translation="MNLLILGSGGREHALAWKLSGSRKVSRVFIAPGNAGTAECGTNL
                     DISPDNFAGVRQAVIENGIGMVVVGPEAPLVSGIHDFFLEDDILRTVPVIGPVKKAAM
                     LEGSKDFAKAFLTRHNIPTSRYQSFGKSSEKEAISFLGTMDPPYVIKADGLAAGKGVL
                     IIDDIAEAENEIRAILGGKFGNAGNRIVIEQFLRGTELSVFIATDGRSYKILPEAKDY
                     KRIGEGDKGLNTGGMGAVSPVPFAGKVFMEKVRTRIIDPTVNGLIEEGIEYKGFIFFG
                     LINVDGDPYVIEYNARLGDPESEAVIPRIRSDLFELMEGIAQGNLGEREFLTDERFAA
                     TVMLVSGGYPGSFGKGYKIEGLDRTSGCVIFHAGTKNEEDKIITSGGRVLSVSAWGNS
                     MGEALDLCYRNAALISFTGKYCRNDIGFDLNNNAE"
     gene            complement(26097..26984)
                     /locus_tag="PK288_00885"
     CDS             complement(26097..26984)
                     /locus_tag="PK288_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM17499.1"
                     /translation="MSAIPKSMIDLQKPIPSYLNEKSNIVRLILFTSLFALVFINIYF
                     PFGADRWFDLTRPEFFTYSSLVILTGVLIVVISRVIMYYRGRKNIIRLWQYLAWIAGE
                     IMLMALFYALFEKFVLEDSRDFVFLVKLSARNTALVLLLPYSVMWLYLSWRDKNQQIE
                     RMSDMPAVPDGTRDMIPFYDEKSILRFSVKKENLLYIEAAENYVNIFYLNKGKIARYL
                     LRDTLKKMEEKFTDTGIIRCHRSYMVNFEKVRVIRKEKDGLKLELEISPVPDIPVSKT
                     YVDNVMQTFSKYSRMTDNS"
     gene            27184..28314
                     /locus_tag="PK288_00890"
     CDS             27184..28314
                     /locus_tag="PK288_00890"
                     /inference="COORDINATES: protein motif:HMM:NF040309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6051 family protein"
                     /protein_id="HPM17500.1"
                     /translation="MDYITSFSILKSLYSINLKEVTIPGSELKILNIEFRSESNPEGL
                     MNDEDLSITENNFFTYPVFVPPGRVSGKVVLLLHGLNERNWIKYLPWAYHLSVNTGSY
                     VILFPISFHINRSPSSWINPRLMTGPVKERNDKCSDVRMSTYANIALSSRLSQDPMRF
                     FHSGCQTAFDIIKLVQQIRSGNHEVIPAGSGINIFAYSIGAFLAQILVMGNPENLFTD
                     SKLFMFCGGSVFSSMKGTSRLIMDSEAFNRIYNFYLDDFENEIRRKSSKYGSISESQL
                     GMAFRSMIDFGRFRSFRDNLFEKLRGRIRSVALSRDSVIPAEGIVRTILKNRKPGSIV
                     EVWDFPFEYSHENPFPVHIRSDERSVNSWFSRLFSEACLFLA"
     gene            complement(28274..28921)
                     /locus_tag="PK288_00895"
     CDS             complement(28274..28921)
                     /locus_tag="PK288_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM17501.1"
                     /translation="MRQYRPFFLIRKIFTKAIFRIPTDEKLLCLTFDDGPHPDSTVAI
                     LDILDKYQVKATFFLTGANAVKYPGVKDMITAAGHMTGNHGYRHISGWKTSSSRYADN
                     VSRAAGPVPSRFYRPPYGRLTLRQYNQLIREYIVVFWDLMPYDFSGSQSAAKCLSILR
                     KRIRPGSIIVLHDSPDSKVTGFLAEFIEYASGEGYRFVTIDHAMPGTGMLRKTTC"
     gene            complement(28927..31992)
                     /locus_tag="PK288_00900"
     CDS             complement(28927..31992)
                     /locus_tag="PK288_00900"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPM17502.1"
                     /translation="MNSVWGSFRRINMITGWIIFLFSSMIYLLTMEPTVSFWDCGEFI
                     LSAFRLQVGHPPGAPLFLMIGRIATLFAGGDTSKVALMMNAFSAISSGFAIMFLFWTI
                     THLAAKIQGKENEVEKKQIPAVIASGVIGAMAYTFSDTFWFSAVEGELYALSSLVTGL
                     VFWAMLKWEEEADQPTASRWIILTAYIMGLGLGIHRLNLLVIPVLIFVYYFRKYEVTR
                     AGMIKTFLIAAAVLYLMVFILIPGVPKVAGWFELLFVNVLGLPYNSGLLFAVFLIFAV
                     LFYGIWYSIKREKTVLNHVLTVLAVIMIGYSSYAMIMIRSAARPPMNQNNPSDIFSLS
                     YYINMEQYGSSPKLYGNYYSAPVIDVKNKIAGYNKIDGKYKPYFRPDYEYKKEFMTVF
                     PRMYSPDPEHENAYKYWGKITGRRYVVSSGSGNETLICPTFAENLRYFFRYQVGFMYM
                     RYFMWNFVGRQNDFQGNGNPIHGNWISGIDFIDKARLGDLDNLPEDLKNNPGRNTYYF
                     LPLLLGIVGLLWHYRNNVNDFWLVMAFFFMTGIAIIFYLNQYPNQPRERDYAYAGSFY
                     AFSIWIGLGFMPVYSLLKKFLREYLSAAIAFIILLAAVPVLMAVENWDDHDRSGRYTA
                     RDIGANYLKSCAPNSILFTYGDNDSFPVWYIQDVEGVRTDIRVANLSYLQAGWYIDMM
                     RQKAFDSDPMPLSIPAEKYIEGVRNQLPVNSRLEKPVELSQIIQFAIQDDRKYMIDIN
                     GRGNYLNYLPANKFIIDVDPEVVLANGTVKEYYRDRIVSPMIWEYTREDAFKGDLAIM
                     DLLATNKWARPIYMSTTVPSSQYKGLEKFFVQEGMAYRIVPIRTTKPEAGEFGMIDPV
                     VMYDNMMNKFGWGNAADPGVYLDENNRRMLSNFRRIFGSLGNALLSSGDTLRAVEAVK
                     RGLEIVPASKMPNDYFSVTLAEVLLKAGRKEEAEKVIDDILNYSREYLEYSVNLKPEL
                     RFGMDYATGINMQSYLDLYNMAVKLKLDPLLEKIEPEVSSFYNLLYSNH"
     gene            32121..33227
                     /locus_tag="PK288_00905"
     CDS             32121..33227
                     /locus_tag="PK288_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17503.1"
                     /translation="MRKYGVFILVLIFGLQQLHCMNSPDTSSAGSLGMRFRNISFVRN
                     NEYSNPITEGYTLIGYFIQPELVYSPSDRLRLSLGIHLLQFSGTNGFHLVKPVFTSSF
                     NLTGNMVFTMGTLQGSDSHRMFDPHFSKERLYNAFAEDGLELRFLNDHLFNDVWLSWE
                     NFIFRGDNEREIFTAGESFSYSSEKIGRSIRIRIPVQMMFKHYGGQISDYPEKVETYF
                     NLAAGAGLSYDIADRDFGETGIEAVFYYGKSLTGKPSSGIGRGTGAWYKIRYSYRDSE
                     IDAGLWTSDDFFAPNGNYIFGSVSDHFHNYVIHRRRLLTCSASIRLVHKSFLEFFLGF
                     EGFWDTDLRRFDNAVSLHLRFDKLISLARLKDQR"
     gene            complement(33218..33766)
                     /locus_tag="PK288_00910"
     CDS             complement(33218..33766)
                     /locus_tag="PK288_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPM17504.1"
                     /translation="MTWKAKSFIRLMASNKRLGRDFYLRDVLEVAPELLLKTLVMADG
                     KGAMARYVITETEAYRGPEDKACHASKGRTNRTEVMYHDGGRLYVYLVYGIHWMLNIV
                     TGPSENPQAALIRSVKDFTGPGRVTRALGIDRSFNGEDLVSSARIWIEDSGIATGYKT
                     SERIGIDYAGEYWKTRPWRYYL"
     gene            complement(33738..34208)
                     /locus_tag="PK288_00915"
     CDS             complement(33738..34208)
                     /locus_tag="PK288_00915"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPM17505.1"
                     /translation="MDIDRSFIERAITLATESVRSGGGPFGAVITRDDRLVAEAGNRV
                     VLLKDPTAHAEVLAIRKAAKALGTHKLTGCVLYASCEPCPMCLAAIYWSGIRRVVYSS
                     DRNDAAAAGFDDRDIYGEICMDQAERSVSFRQVHVQGAGEPFKLWNDMESKIVY"
     gene            34520..34963
                     /gene="rplM"
                     /locus_tag="PK288_00920"
     CDS             34520..34963
                     /gene="rplM"
                     /locus_tag="PK288_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPM17506.1"
                     /translation="MNTLSYKTVSANKATVNKEWVLIDAEGAVLGRLATVVASTLRGK
                     HKTNFTPHVDCGDNVIVINADKVSLSGTKWSDKEYVRHTGYPGGQKFTTATEMLRKNP
                     ISLVEHAVRGMLPKNRLGRAIFKNMHVYAGASHPHEAQKPKIMEL"
     gene            34982..35368
                     /gene="rpsI"
                     /locus_tag="PK288_00925"
     CDS             34982..35368
                     /gene="rpsI"
                     /locus_tag="PK288_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPM17507.1"
                     /translation="MESVNAIGRRKAAVARVYVSEGKGNITINNRDYREYFGADTLQY
                     VVTQPLMVLNASDKYDIRVNLEGGGVKGQAEALRLGIARALILIDPENKPVLKANGFV
                     TRDPREVERKKPGRPKARKRFQFSKR"
     gene            35482..36372
                     /gene="rpsB"
                     /locus_tag="PK288_00930"
     CDS             35482..36372
                     /gene="rpsB"
                     /locus_tag="PK288_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPM17508.1"
                     /translation="MPRTNFQELLDAGVHFGHLKRKWNPAMAPYIFMERNGIHIIDLE
                     KTIVKLDEAASAMKHIARSGKKILFVATKKQAKEIVAERVKQVNMPYVTERWPGGMLT
                     NFPTIRKAVKKMGSIDKMASDGTFSNLSKREKLQVTRQRAKLEKNLGSIVDLTRLPSA
                     LFVVDVCKEHIAVKEAKRLGIPVFAMVDTNSDPTEIEFPIPANDDASKSISLIVNLLC
                     QAIEEGLNERKLEKDKEPVQKDRKVIRKDLETKDLPETVIAALSDEEDDDEPETEEET
                     DDELETDDETTVEADETDEE"
     gene            36409..37239
                     /gene="tsf"
                     /locus_tag="PK288_00935"
     CDS             36409..37239
                     /gene="tsf"
                     /locus_tag="PK288_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPM17509.1"
                     /translation="MATINAADVAKLRRVTLAGMMDCKKALEESNGDFDKAVEIIRKK
                     GQAVASKRADRDATEGVVLARTSADGKFGAMIVLNCETDFVAKNAEFISLSDKIIDTA
                     LVSKPENLEELKALKIGNITVAEKVLEFLGIIGEKLELSLYSKIEAPHVQAYVHPGNR
                     LATLVGFSREGLDLQVYKDVAMQVAAMNPVSISKDDVPENIIAQEIEIGKEQARRDGK
                     PENMLETIAQGKLGKFFRENTLLGQEFIKDNKITVSQYLQSVDKTLTVTAFKRFSLNL
                     "
     gene            37473..37688
                     /locus_tag="PK288_00940"
     CDS             37473..37688
                     /locus_tag="PK288_00940"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPM17510.1"
                     /translation="MKTNLGNTDKIIRTIIAALFAILYFTGVVKGVIGLILLILAVIL
                     LLTSIIGFCPLYTLLGLSTKGKEPEKK"
     gene            37856..38563
                     /gene="pyrH"
                     /locus_tag="PK288_00945"
     CDS             37856..38563
                     /gene="pyrH"
                     /locus_tag="PK288_00945"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPM17511.1"
                     /translation="MKYSRILLKLSGESLAGEENHGLSDVMLEEYASQIIEVGKKGCQ
                     VAIVVGGGNIFRGLSGTRSGYDRVKGDQMGMLATVINSLALESAITKLGGKAKVFTSV
                     RMEPVGEFYNRDKVVACLNDNIVCILSGGTGNPFFTTDTASALRAVEIGADVLLKGTR
                     VDGVYNTDPEKDKTAVRFERISFDEAIDRQLKVMDSTAFTMCRENNMPVVVFNMNVRG
                     NLMKAVSGDNIGTLVCN"
     gene            38674..39237
                     /gene="frr"
                     /locus_tag="PK288_00950"
     CDS             38674..39237
                     /gene="frr"
                     /locus_tag="PK288_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPM17512.1"
                     /translation="MNEEVEMIIEETRERMQKALDHLEHELARLRAGRATPVLLDGIT
                     VDYYGVNSPLSQVSNINTPDPKTILIQPWEKTMLGAIEKAIMAANIGLTPINNGEIIR
                     INIPPLTEERRHQLVKQVRTEGETAKISIRNARKWANDELKRMLRDGLAEDIEKNAAE
                     TVQELTHEFSGKIDKVMALKEKDVMTV"
     gene            complement(39240..40121)
                     /locus_tag="PK288_00955"
     CDS             complement(39240..40121)
                     /locus_tag="PK288_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HPM17513.1"
                     /translation="MPAAISTELTNHCNLNCPECSSGSGIMGRKRGFMDIGLFRDILA
                     EMKPYLCNINLYFQGEPMLHPLFFSFLESARGIRTTVSTNGHYLTAENSDYLAGSGLG
                     RLIVSVDGMDQAAYASYRKNGELDRVIAGVNNVVAARKKLNSSMTIEIQFLVNRYNEH
                     QIGQVRQFAKERGIRLRLKSMQVINEESIQGWMPEIEKFRRYEKGDGKYIIKSTLPDN
                     CRRIWFNPVVTWDGKVLPCCFDKNADHVMGDLKKTSFRAIWHGAEFDKFRHRILNNRS
                     GIDMCRNCTEGLKGVKT"
     gene            40354..41655
                     /locus_tag="PK288_00960"
     CDS             40354..41655
                     /locus_tag="PK288_00960"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM17514.1"
                     /translation="MKTFRSLSFLPLIIISLLAGQTLNAGVHPGNGKNDQQTVLTFRG
                     KVVDGETGTPLVFASVTVKETNVATITNIDGEFVIKIPESDASKNLEITFLGYRNKII
                     SISELRENGRKNTISLSPAPIPIKEIIVRPLDPEEIVRNAIYRISRNYPNEPNLMTAF
                     YRETIRKNKTYVSIGEAVVEIFKAPYSNDLRFDGARIYKGRKGTDVSRMDTLLFKLQG
                     GPVNTLQLDIVKNTETVLTLEAMKNYDYSIAGVIEIDEKPHYIIQFMQKPYVENPLFM
                     GNFYIEADSYALTEAEFGFNLRNIEAARSIFIKKKPLGVEVTPEVATYRTRYREQDGK
                     WYFAYSRAEVKFKVNWKKKLFNTYYTTMSEIAITDRTTEEVIKIAGKEKIRPSDVFSE
                     QVEAFTDPEFWGDYNVIEPDQSIEAAIRRLSRKLRFSDRKD"
     gene            41677..42213
                     /locus_tag="PK288_00965"
     CDS             41677..42213
                     /locus_tag="PK288_00965"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HPM17515.1"
                     /translation="MIRDSEIIGRIRKGDVSQFESLFRSCYVSLVKYAKTIIKDHDTA
                     EEIVQDLFFRLWKERENLSVKISLNGYLFRAVHNRCIHHIEHEKVVGKYAEEMLHSGS
                     NSPEIASDILIYNDLQTTVARILERLPWKTGRIFCMSRFEGLKYSEIAEKLSVSVKTV
                     ESNMGKALREFRKILAEQ"
     gene            42210..43217
                     /locus_tag="PK288_00970"
     CDS             42210..43217
                     /locus_tag="PK288_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPM17516.1"
                     /translation="MKTKYEMLKYTDKEWAELASMLSDEKTGQGELLSRFVSDDKSGV
                     IKNWKEMQKFSNDKEINVDRAWNNLYGRLSEDKLIGKPVTGKKLIAGNAFLRMAAGFL
                     ILVSLGVASYLIFNHRTTDSAIVVTTEMNQKNLQVTLPDGSTVCLNRNTSVAYGHNFG
                     KTDRKITLSGEAFFNIVPVPDVPFVVDAGKAAVMAKGTSFNVISSNSANAVEVYVLTG
                     KVVLSDNSGNRSIELEPGFIGTMKKETSSKTLNNDPNYLAWNTGILVYDGQTLETVFN
                     DLRKVYNMEIVADDPGILQKTWTSPINNQPQETIIRLICTSFNLNFTKVGNTYHLSAV
                     K"
     gene            43233..44780
                     /locus_tag="PK288_00975"
     CDS             43233..44780
                     /locus_tag="PK288_00975"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM17517.1"
                     /translation="MSAGSGYISKNIPAIFLILFLIPQEITGQNAILDSIFTFNAGKI
                     KTGDALDTISRQTGYSFTYDSRLIDTEKPTDLNFSGISLKSVLKSIFQNDSLNYTIID
                     KFIIISRGIEKKISDRDSTVISEIRSVTGLILDAESGDPMPFATIGLKNEGRGTVSNN
                     NGEFSLKIAPAYLRDTILISYLGYLGKEIPVAEALTNNLRIMMIREFISIPEIIIRNQ
                     IPQEVINRALNLIPKNYGTSPALLTGFYREGVQKRKKLQSYSEAIIRIFKSPYSVTFL
                     NDQIKVLKSRKIENISIKDTLTVRLKAGLSTCLQLDGVKSAFEFISDANMHEYNYRIT
                     DIVSFDDEAAYEIEFEPRGEVDLPKFKGSIFINTSDYAILRADFELSSRFLDEMKEAF
                     ISTASKGFRTWPISVRYSVSYRNVNGRYFLNHVRGDLLFLSREKKRLFNSPFEVFFEM
                     AITSMKTDNVVRFEREELAPIHSVFSRTISNYDSEFWGDQDFLKPEENLLHALRNMKV
                     RLQQFPE"
     gene            complement(44848..45861)
                     /locus_tag="PK288_00980"
     CDS             complement(44848..45861)
                     /locus_tag="PK288_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM17518.1"
                     /translation="MKKTTIPSLACIILLLSAISCVSPKKYRELQITSRQNMMDRDEF
                     KAENIRLQMLNRELDNNASALENEIKEMKYRLSTAESERNRAREDLSIVESRLNDMQN
                     AHQDLIRGNVNETKKLLAELISTQENLRQKENILRELETSLDLKKISLDELTYELEKR
                     NARMAELEKILDAQKKLVQDLKGKVSEALLGFENNGLSIAARDGKVYVSLDEKLLFRS
                     ASWDIDANGRKALRNLAEVLERNPGIQITIEGHTDNVPYNPGNSQLKDNWDLSVKRAT
                     TVVRVLLEGSRIRPERLTAAGKGEYAPLDLNNTPEARQKNRRTEIILTPDLTELYKLI
                     NKY"
     gene            46025..47950
                     /gene="dxs"
                     /locus_tag="PK288_00985"
     CDS             46025..47950
                     /gene="dxs"
                     /locus_tag="PK288_00985"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPM17519.1"
                     /translation="MNGGEHLLWRISTPDDLRKLKPADLVKVSAELRQYIIDVVSNNP
                     GHLGASLGVVELTVALHYVFNTPYDKIIWDVGHQAYGHKILTGRKEKFLTNRKYKGIS
                     GFPKMSESEYDAFGTGHSSTSISAALGMAVASKRKGEKRNVVAVIGDGAMTAGLAFEG
                     LNNAGIAKSDIIVILNDNNIAIDPNVGALKEYLLDIATSKAYNRFRDRIWNMLGKFGR
                     IGPNARVLAKQVQAGFKSTILDRSNLFEGMNFRYFGPIDGHDILHLIQILEDLKRIPG
                     PKLLHCLTVKGKGFKKAEENQTAYHAPGRFDKNTGIIIRSSAKDSLVTYQEIFGRTIL
                     ELAKNNDKIAGITPAMTTGSSLCIMMKEIPDRVYDVGIAEQHAVTFSAGLATQGMIPY
                     CNIYSSFIQRAYDQVIHVVALQKLKVIFCIDRGGLVGSDGATHHGFFDLAFMRSIPNI
                     IVSAPMDGPELRNLLYTAQLEKITGPFSIRYPRGNCMISDWQKPFFEIETGTARLISS
                     GRDIAVLSIGHPGNFVVEAGRILAEEGISVTHYDMRFLAPLDTRALHTVFREFGKIIT
                     VEDGILKGGFGSAVIEFMSDNGYKAEVKRLGIPDYFVEQGTQQELIRECGFDPESIAQ
                     TIREMVMKRVPEHKLKA"
     gene            48044..48826
                     /locus_tag="PK288_00990"
     CDS             48044..48826
                     /locus_tag="PK288_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPM17520.1"
                     /translation="MRNLLVITVILLLISCGTRQQPADQEWIQLFNGKDLNDWSVKIK
                     GYKLNDNSRNIFRVEDGVMKVRYDDFDKFRDEFGHIFYKKPFSRYKLRIEYRFTGDQV
                     PGAPAWAYRNSGVMLHSQSPESMGVDQDFPVSVEGQFLGGDGINERSTGNVCTPGTNI
                     VMDGELITQHCINSSSRTYHGDVWVRAEFVVLGDSVIHHIIEGDTVLTYFKPQVGGAP
                     EDYPVPEGTLLNQGYICLQAESHPVEFRKVELLDLSKGRKQK"
     gene            49041..49124
                     /locus_tag="PK288_00995"
     tRNA            49041..49124
                     /locus_tag="PK288_00995"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:49075..49077,aa:Leu,seq:caa)
     gene            complement(49717..51036)
                     /locus_tag="PK288_01000"
     CDS             complement(49717..51036)
                     /locus_tag="PK288_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM17521.1"
                     /translation="MIKIPAIVCLTIWLLPAGIKADNPENQVVSNDSLSLEILIEEII
                     KNHPSVKEAEETLKNADARISLARTGYYPVIDASAGFSNIGPVLKIDIPQIGEMQLFP
                     ENNYSGAVNYRQIIYDFGRTRQNVKLETGNKTISTQTLELVKQKMSLAAINGYYSILY
                     LQEAVKIKNEQLATLEEHLRYIRAMQATGSATDYQILSTEVRISAAQSQKTDLLYSLT
                     VQQAYMGALIGIEKFKRPVMKDLGIELPLINTGSLQQYALLHRGEILISKENASLAEL
                     QYQLLRTANKPLISMTMAGGVKNGYQPDLNTLRANYSAAFGISIPIFDGMKTRYKLRQ
                     AQSVINNAGYETENIRRSIISEIQEAQSYLESAAQKISQSALQFEQAKKAYELAGISF
                     RAGTITNLEVLDANTAVSESRLILLKSRIDYAAGVYRLKAALGEKLY"
     gene            complement(51038..52660)
                     /locus_tag="PK288_01005"
     CDS             complement(51038..52660)
                     /locus_tag="PK288_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HPM17522.1"
                     /translation="MIKRQPLLHRIRAFFRKPDSVIHPKHLKYKWFILGNVMLGTFMA
                     VLDGTVVNTGLPKIMASFGTGLDKVQWIITAYMLAMAVMLPTSGWLADRFGYKRMYSL
                     GLSGFTIGSFLCSISPNEDILIVSRIIQGLGAGTLQPLGMAIITREFPVKQRGLALGF
                     WGIAAAASISFGPTLGGFIIDNFSWPLIFGINIPVGIIAILFTIVIQSEYKHPAKRKF
                     DLIGFISITIFLPLTLFALSETTAATNTAGWHAPYILLCAAIALIALTVFITAELTAE
                     DPIIDLRLVLNHNFGLALIILVFFSIGMFGSTFLFPVYLQNSLGYTALEAGAVFIPVG
                     IIQGIVAPISGRISDRINPKIPIIIGILLMSFSFYLNSKLSYLSEIRSIMVSLYLRGF
                     GMGLTFTALNTVALLEIPRAKMAQASGISNSIRQLGGSLGVALLATMLTVRTIYHNQQ
                     FGTALNARSQAYNVTTDRLRFHLQHNAGIPPVKVESAASSLILSNLNKQAYIEGINDD
                     FLLSGIFTLIGGIPIFFIRTKKSNAHKNFTQE"
     gene            complement(52662..53669)
                     /locus_tag="PK288_01010"
     CDS             complement(52662..53669)
                     /locus_tag="PK288_01010"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HPM17523.1"
                     /translation="MRQKSNKHNIKIYIPLGLVIVIVLTGAWFWYRDYSSFISTDDAH
                     VDADNSAVSSKIPGRIVSLFADEGDRVKKGQLLAVLDSTDLSAQKVQALAVKNQALVS
                     VSQSGFKLHSDRQGLKVVKISYEKAWEDFERAEQQLEGGVITREQYDHALKASEVAAA
                     QVEAARALLSVSEAQVKSAYAAAVTADAQVKVIETQLGNTRLYSPSDGVISKRWLLPG
                     DIIQPGQPVFTVTDDGNKWISAYLEETKIAEIHPGQSVRFTIDAFPHRKFHGKVFRIG
                     ASTASVFSLIPANNASGNFTKVTQRIPIRISIDSVSNGNELPSDGILSGMSAMVKISR
                     K"
     gene            complement(53699..54316)
                     /locus_tag="PK288_01015"
     CDS             complement(53699..54316)
                     /locus_tag="PK288_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM17524.1"
                     /translation="MKSDDKQKENKAEAIIAAAQKRFALFGIEKTPMREIARDLRISK
                     AALYYYFPDKESLYKSVLTREHDEFLRILHTEIENSNDPVDNLRKFSQSRISYFRKLS
                     NLGRLTLDSYDHLKPVIANLLEIFREEEKRTLVLILEKGIRQDLFRIDDTYKIATLFL
                     DIIRGQIKLILSGKDLLMADDAEFTLLSEKVKDMSDVFIKGLLYK"
     gene            complement(54802..55335)
                     /locus_tag="PK288_01020"
     CDS             complement(54802..55335)
                     /locus_tag="PK288_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17525.1"
                     /translation="MKRVTLSACILSVLFTLFLLNDLNGQTGDKGTVDKPDYGKNIVY
                     TPAPPPETPPEATNLGQQPADMLKQITYLDSQVVPGAYYAECSWLYKAYPDIVWVTEH
                     THDADEVFGLYGSDPDNPNELNGEIELWIDGERHVITKSCLIFVPKGVKHCPLKILRV
                     DKPIFFFTTMPAHKYAF"
BASE COUNT        15550 a        12687 c        12268 g        14924 t
ORIGIN      
        1 cccctaacct ccacttcctc acatttaaaa taaaacccac aggggttccc tgcccgactg
       61 cctaagggca ccaggcaggc ggggattccc ctaacctcca cttcctcaca tttaaaataa
      121 aacccacagg ggttccctgc ccaactgccg aagaaggatt atgatcatta ctgacatcaa
      181 gccctgaacg ggcgcaatat gaactggttc tttattattg cctgcccttg aatatcaggg
      241 aatattttat ccgtaacaga taccgccgag gataatgaca tgacgctcct tcagagctga
      301 aaagcacatg gctgacctgc cccccgccgg cgccttttcg tgatttttta ttctgtcacg
      361 gaattcagat ttttgcttaa gtgcgggatt tttgccgttt ttctatttta acattataat
      421 cacccacact aaccagtgta attccgtttt gcttcccgag gtttcctgaa tattgcatgt
      481 caaccggatt gttccagtgc tcgtggacac caagactggt tagaggtatt ccgctgttgt
      541 cggggtcgta aatcagtccg tccggccagt tcttcggatc agcggcctga tgcaggtgat
      601 catcaactcc cggcatattg ggaacccatt cccaaactgc atttgcggga tcgtgcttgt
      661 tatttccgtc ccattcggtt ctcagaaaat caaagcagac ggattcaagc gccacctgat
      721 cctgcgacat gaatattgaa gaactccagt gaccgttgaa aggaggcata aagtatttaa
      781 cgggaccttt tgtttcatcg gatccgcccc cgaacattcc gtcaacaatg aacagcattg
      841 tattccggcc aagatatttg cttcccatca ggtcaaccat ggccctgtat tttctgtaac
      901 cgcggttatt gggattgcca acctgcctgg gtgatgtttc ccatgagggg gccacggtcg
      961 aatagtgaag acgcgaggcc ctgccggcaa ttgatccgaa atggttcttt gtggtaagag
     1021 atattcctgc cgaaccatgg ggcttgaagt tggccagatt aatcatgtaa tccgcatctt
     1081 caataacatc aaaaagcctg tccgtcattg ttttgtcaga ataaaagaga aggacctttt
     1141 cggtttcctt aaccacggta cggttaaaag catcagagta cttgtcggta tatcttatac
     1201 caggaaattc tttatgccag tatgaataat tatgcccgaa gatatgcttg tgcggatcgc
     1261 cgattgatat atccgactgg tttattccgc attcattgac aagttcccgc aggagttcga
     1321 ggacaatgta gggaccggtt tccatagcgc cgtacccgcg cgatcttgca cccggtttaa
     1381 gcggatagga ggggacaaaa taaccgtttg ccttatcttc cctgctcagc agccagtcgg
     1441 aggttccctg gttgattttt atataaatct tttctccggg ggtgtatccc ctgtctttgt
     1501 tgtgcttttt cagattgtga taacgaaaca gctggtccca tgccttattg agtttactct
     1561 ctcctgtcag cttaagtatg gactccgaga acatactgtc aatgatcttc tgatcgaaat
     1621 tcaccggatt aaaataccag ttgttgtttt ctattacatt cttacaatcg ggatttgtgg
     1681 cgccgggatt ccatgcccag accacccgtc cgggattacc gcccagagcc cggcccatcg
     1741 gctggttggg cccgtcttca ggtcccgcac tgtttgaacc atcctgctca aagaaaatac
     1801 ttggggcttc caacaacttc ccgtgatgaa acagacctga tgcctcttca atagccttgc
     1861 ccggataaac ataagttgcg gcagtcaata cgccaaccga ttttacaaat tttcttctcc
     1921 tcatattatc atgaatagtt aagttatcat attgtcccaa acaagcctgt agccagttat
     1981 caccaaaata agtatttctg aacaatgacc gggccgatag cggacatgtt cctgctgtcg
     2041 gtgcctgact tacttaaaac cctgtctttg tcattttagt ctggtcataa aggtaaaaag
     2101 aatccattca tctgaagtcc ctgtgcgata ataaatgtcc gtttccccgg attttgcatt
     2161 gatatattaa taattccctc tcaaaacagg ctttttgggc aatgcagttc caatgaaaac
     2221 cgggaacaag tccgggaaaa gtcacggatt caggattgaa ttaacctgat ttaacatgaa
     2281 acagcttgtc agttcacagg tttgtaataa cttaataacc ggaaatcaat ttacaacccg
     2341 aaccatggaa aaagaaatta atgcccttta ccgggcttat aaggacggaa aggattcaag
     2401 aaggtctttt ctccgcaagc ttgccctgat cacaggaagc agcgccgcag ccgtggcact
     2461 atttcctgtt ttggaggaga atgatctgaa agccgcagga acaaccggga gagcctctcc
     2521 ggagctttca atcggatttg tgaaatatcc tgcagaaacc ggcgacatga gagcatatca
     2581 agccaggccc aaaaaaggaa agaagctccc gggtgtaatt gttattcatg aaaacagggg
     2641 cctggttccg catatccagg atgtcacaag gagaatggcc ggggaaggat tccttgcact
     2701 cgcgcccgat gcactgtcac cgcttggagg aactcctgac gacacaagta acgttggtga
     2761 gatgttcagg aaactcgaca gtgaaaaaac cacgaaaaac tttgttgctg cagtcagata
     2821 tctgaaaacc aatcctcttt gctccgggaa ggttggatgt acgggattct gctggggcgg
     2881 ggccatgaca aaccaggtag cagtgaatgc acccgatctt gatgccgcgg tgccgtatta
     2941 cggaagacaa ccggctccgg aggatgttga aaagataagg gcacccatta tggcccatta
     3001 tgcagggaat gacccgggaa tcaatgccgg gatccctgcc ttcgaggaag ctctcaaaaa
     3061 atacggcaag gaataccaga ttttcatgta cgatggtgcc atgcacgctt tcaataatga
     3121 ttccaaccct gagagatata atgaaaaagc tgcaaagctt gcatgggaaa gaacggtcgg
     3181 attcttcaaa gaaaaactga aataagagcg ggataagtta ttgttcgtct aagctgttca
     3241 tttcctgttc cacccttctg ccactgccct gacaaaagga aggcagtgcc ggaaaaatgc
     3301 cttatatccg gagcacaggt aattcaatcc cggctctcca tcgggagctg taataaacct
     3361 gttcttcgga cattctccgt tacacatggt ccttacatcg catattttgc aataatacgg
     3421 aagtgaacat gacttggcaa ggccgaaagc tttctgcttg tcactgtaca gaagtccggc
     3481 taccgaatta ttcccgatgt tgcctacgag attttcgctg ttcacaaaat ggtcgcacga
     3541 gtaaaaatcc ccgtttgcct caataaccgg gaccgctccg caattttccc tgaagatgca
     3601 taaggtatgt tcctggttga acgcggttcg cgctgcttct tcgaagatct ggattttgat
     3661 cctcccgatg tccttctcaa cccactcgtc aaatatcgct atcaggaaac tgccaaatgc
     3721 atcggcactg actgatgccg gggaaacacc tcccggcaga tcaggccggg gctcaacaag
     3781 tggcagaaag gtaatatttt cagctccgat attcctgaaa aaatcataga ctgccaatgg
     3841 atactctgca ttgaatgaat ttacaacaca gaggatttcg gctgagacgc cttcctgcat
     3901 aagcatttca aagcctctca gggtctcaga aaatgaagtc cggcccgaag ggccccttct
     3961 gaaatgatta tgcagctcgc ccggaccatc catgctgacg ccaactatga aattttccat
     4021 cctgaataac catgtccaat cccggtcgat caatgtagaa ttagtctgta tcccgtttat
     4081 tgctgacttt ccccggggca aatgtcttct ctgaaattca agggctttcc tgtaaaagtc
     4141 aatacccgcg agcaacggct ctcctccgtg ccaggagaaa aagacaacag gcccctccgt
     4201 ggcctcaata tgctgaataa tatatttccc gagtatctca tcggtcatca gcaaatctga
     4261 tccgtcatgg taaagggata atttatcaag gtaataacaa tatcggcacc ggagattgca
     4321 tctggcacct gcaggtttga cgaaaatctg aaattcaggc aggtaatttt gtgtttcagt
     4381 catcccattc aaatttttcc ggaaagtgtt cagggcagtg tatcccgggt agcagatctg
     4441 cgccgttgtt ctccggatta tttgataatt acagatcgcc ggatataacc attaccggat
     4501 ctggaagtcg cggtcaaatt atcgttttaa ttctttaaat cctgaccgtg gtattctaat
     4561 ttgttttttc ttcattgcag cctgttgtaa taacacggga gttgtcatct gagtatattg
     4621 actcaatccc atcctccgga ttacaattgc gaacccgaag cgaagaattt accgctccta
     4681 tctgcgaaat cagctttacc gtccctaagc tgttggttaa aatattgctt aataccacat
     4741 ccctggaatt gttgcagtaa acagcgtggg tccagaagcc tccggcattg gaaaaatcga
     4801 cggagaatcc atccacgttt aaccctttta cattagcgac ataaagcgca caggagcttt
     4861 cactattcca tacagtaacc tcaagcggct gatctttctg aacttttcct gacggaatta
     4921 tattaatgtt cgacaggttg atattctcaa tatgtttttc catagtactg ccaataaagc
     4981 ttgttccgcc gcttgttgca atcaggtttg aaatccggat gttcctcatg aagcatgatg
     5041 attcaatatc ttttttatca ttgcccatcc acaagtaaat cggttgggtt acatcctgca
     5101 ttacaatgtt gtcaaactga atattttcaa ctctcgtgcc cctgattata ggaaagtcat
     5161 cgcccctgtc gggtaatatg ctgattatat caattccatg attggatctg tacatggcca
     5221 gattgctgaa tgttgcattc cttatgggtg aatcaccttc ataacccacc cgaatggcac
     5281 aggccggggg agagctcaaa atacagtttg tcacaacaat attttcacag ggtcgttcct
     5341 catggcccaa ccttcctgaa tcgcttttta ttgcgattgc atcatcgccg ccttctatgt
     5401 tgcaatttga gattgatacc cggcggcagc aatcaatgtc aattccatcg gtattcggac
     5461 catcaagggg attccggatt gttatgcctt caatgttaat atcatcgcaa cccaagggcc
     5521 agagtgtata gcaaggtgaa ttgttcaact gaatatcgcg gatgattata aatttacagt
     5581 ttacaaaaca aatcaggctc cgtggtcgcc attccctggg ttttcttacc catttagtga
     5641 acatttcctc ttcccagaaa gtatccccgt ttccattaat ttctcccctg ccttctatgg
     5701 aaatatgttt tgcgttttcc gcataaataa ggaatgatcc ttcagctccg aaggaaggtt
     5761 ttccttcaac ggtcagtaat cccgctatgg gctggtagtc ctggcggttc ctgcttccca
     5821 taagtacggc atcagcgtcc atgtgaactg ttacattgtc cttaaggaac aatgtccctg
     5881 aaataaaagt cccccgggta aaccgaacca ttccacctcc tgcccggtaa caggcatcta
     5941 tggctttctg aatggctgac gtgtttaatg tcaaaccatc tcctttggca ccaaagtcag
     6001 ttacatacca cactctgttt tccggtatcc tgacggaggg gtgtttctta tttttatcct
     6061 gtgccttagc gcacaagctc caggaaaaga tcagaattag aattacaatc agtactttgg
     6121 ggaatttcat gttccttaca attattttta cagataactt ttaaatatac gaatgatttt
     6181 ataaagcagg cttccgggca ggcaggatag tcatcctgcc ttcccggaaa tgattcgtgt
     6241 aatcctaaga aaagttgatt ttattaatgc taaaaatgag ccgcaattaa agcaaaacca
     6301 tttttgataa attcacggtc cttcaggatc tcctgctatc tcatagtccg attaaaaagc
     6361 caataaagga ttctttaaaa acaaacatta gtgcccgatt aactacatgt cgctcctgcg
     6421 aagcttaaac aatagtgata tctaagtctt acaaacattt cactcctacg gagttagctc
     6481 tatggaagtc ccgttgggga agtcatgtta atagactttc atcacaaaca ttaaaatgag
     6541 ccccatcggg acggcatgta tttcaatgtc tttaaaataa gtatattagc tcaaaattac
     6601 tcaatttagt cgttcacttt tgaaaaacac taaagaacca ctgcagcttt aaggtctttt
     6661 gcaaggggtt atttggccgg agatttagaa aacccggcaa acaggtaaag ttacatgaat
     6721 atacacccgg atatttcact gtatgtccgc aggtagaact taaactgatc acagcatata
     6781 ttggcacccc ctctgtaact ccaggtacgc agcccccgat tctttacctt gtgcggcgcc
     6841 tgcgaaagga tatttatttt tttaccgcca ggaagtagac cgagttctta atcatggtca
     6901 gatcggtaac ctcgaatccg tacgactcaa taaggtctgc tacgctctcg ctgtcaaatc
     6961 ttttcaggat acccggatcg agaatatcaa ttaatttgtt gtaaattgat gataaaacct
     7021 ccggcttgtc agtcttgctg cctgacgcaa aaaaatgccc catgaggctt gttcccggtc
     7081 cgaccgtttt ggatatgcca tcgagaaacc cgttgagatt ctccatctga ttgagtcttt
     7141 tcagcaatat caggattgtg ggagtttcca gttccgtttg atcgaagaaa taatgatgct
     7201 taggggaaag caataatatg tttttctgat tcaacatatt aagccagcca agatatctta
     7261 tgaagtttga gcctccttct gcaatcaggt tgtctatcgc ctggcaatta atctcttcaa
     7321 tgttctcctt tggaacaacg gacttacctg catttgtatt cttttcagaa aaaattctga
     7381 cttcgcgttt tatggttttg gtatacattg cactaaacta aaaacagccg ctccttcagc
     7441 gggcagccat atattcagga acaatcaatg taatgaaatg ttcagttcgg cttatcagga
     7501 tagcgcagga aggatcaatg gaaaagtaaa ctgaattgat ttggtccata aaaattaccg
     7561 gaacatgggc tttatcccgt catcaacaat cacgtttacc aatcctcttt taacgttcag
     7621 atcccaggtt ggtgccaccc tgaactggtt ggtggatctg atgatcctgc cattgcgcgg
     7681 attaatgaaa tccgctgttc cgtaagcctc tgcacccgaa aaattaatat tgaaattcag
     7741 aatgaaatcc gcattatact ttttttcggt caccgtccag taaccccacc tgttaattgc
     7801 atttgttgcg tgaactacag cattgtcatt ttcacttgta atataaaccc tgttatttct
     7861 tctggtcatt gaataaaaca tatccacaat ggcaggatcg acgggaggat tgtcattcct
     7921 gttattgttt tcaggagctt tcacggaatt aaacacctcc gtctcaccgt tctgataggt
     7981 tatctcactt atttcctgtt tgctcaatac atacacgggt ccgtcgggat tgctgtgccg
     8041 cctgtacttc acttcatccc gactgacttc aagtactttg gccagtactg tttctcctga
     8101 tttcatcttt atcctgtcct gtgaatatgc acagatagct gcaaaaagga atgctgatgc
     8161 cagaatatat ttcatcagta atgtatttat taaatctttc attcaagttt gttccatatg
     8221 aaaaatcagt caaagataag aataattgcc gtttgaccgg catttaccat taagctggtt
     8281 tttatgtcgt tcccgaagct ctgcagtaac aagtgccagc ttccggttaa ataacaggtc
     8341 agcttactaa ctttaaagca atctccgacc ggctgttttt caggagaagt tcagagaaat
     8401 tcaacaatct cgtccagcag cttgcggcct ttttcaaccg gcacctgata tccggctttc
     8461 ggatagccca atggggtgat tgccagtagt tgatgattat catcgatatc aagcgcctct
     8521 ttcagttcaa atggattgta tgccattacc cagcaagtgc caacgccctc atttgcagct
     8581 gcaaggatga tctgtgtcat tgcaattgcc acatccgttt ccattgaatt atatccatca
     8641 taatcccgtg tccaggcttc ccggcgcaat ccggctgaaa tcaggatatg cggtgcatcc
     8701 ctgatccatt cgcgtttgta acacattttg attttttcca gcagtaacgg ggaagacaca
     8761 agataaaact tccatggctg gttgttatgt gccgaaggag cgagcctccc ggcctcgagg
     8821 atcttcctca gaacagcctc cggaaccggc cggtccgggt cataactgcg gatgctcctc
     8881 cgcgtgataa tgagctcggt ataatccatc actttaatat tacatgatta ccctataaag
     8941 ataacaggaa atgccggatt cacattgctc aatcatcggc gctgaaagaa ctgcaattaa
     9001 ccggcactgc cagatgccag aaaatatgtt ttctgaccgg cccggttggt cttgttaatt
     9061 atttaacaca ccgggtttct taacacggct ccggaatagc aaattccgga tgtgtcttac
     9121 tgttaaataa taaaacagac cttattaagt tatttaatta taaatcataa ctatttttat
     9181 tatattttaa aattcaacct gatgaaaaag acagtattat tatttatttc cattctcctt
     9241 ctcggaacga caacagttac aaatgcgcag aaagacaggg ctctcagcaa cggattcagc
     9301 atccatctga tcggaggtct gccttcgggc aatttcggac ttgaaaaaga tgcccatatt
     9361 gacaaaaaat ttaaacttaa cccattgatg ggatttcaga tcgggaaccg ctggtatttc
     9421 actccaggtg aaagattcag ggccggcctg atggttaact ggtttgatct gacatttgca
     9481 tttaaaacta cctcaacaga ttcatttgac tggagcaggt atgttgctga cgtggccgtt
     9541 cttgaattag gacccgtggc cacctttaag attgcaaata atatggcggt agacggttac
     9601 tataatttaa ggccatcagg atttgctaca atgtttgtaa caaacctgtt tggcgaggat
     9661 gagtcggaca cttattccgg gggaggaata tcgcatgcga tcggcggtgc cttcagatgg
     9721 aacattctga gtgttggtgc ggaatatgtt ctgggcggga tcaagtgtaa aaacattgat
     9781 cctgaaagtg atgatacctc gagcaaactt ttggtcaaca gtgtgaggat catggtgggt
     9841 gtaaagtttt agaattctgc ttttttgcag cttaacagga ggcgggtgcc gccggttgcc
     9901 atgtgccttt ttaaagtgaa agagggatct ccttcgcccg ggatgccagg atcaggcttg
     9961 caagacttca tactttctcc gcctcctcga tcattatctg ataattttcc agtgtcctcc
    10021 ctgaaatttc cctgccgtac ggagatgctg tcggcatcag gacaaagccc cttccctcgc
    10081 ctgacgtgcc atcggaaagc gaccttttca cggtttccct gaatttttcc ggcgacatgt
    10141 tttcaatatc tgcaatctca atattgccga acagaaccag ttcccggcca tatttctccc
    10201 tgacttcttt cagcagtaca tctccctggg gaggaggttc aagcggatca acggcatcag
    10261 cgcccatccc tgcaatatat tccatgatat tcctgacccg gccgtgcgaa tggattcgca
    10321 caaaaccgtc gttccttctg atcttttcaa ccagcggccc ggtatgcctg acaacatatt
    10381 cttcaaacaa ttccgggggc agaaaaggct cggtaatgaa ctcgggaccg tatatgcgcc
    10441 aaagccttcc cgggaaatag ccggacactt tctccacacg ctcccagata taccgtgaat
    10501 gtttttccat aagggcatga catagttttt tctcggtcat tgcaaaaaga gtgaaatcct
    10561 caagcctgaa cattgaggca accgcacaga caggatcttc ggtatcgatc atgattatcc
    10621 cccggtcacc cagtttgctt tcttcctgcc tgatgtgctc tgcatcaata ttttcctcga
    10681 atatcacatc gggaagatcc aggaatatct gcacatcatc aattgtcttt atcagcggtt
    10741 caatagtcca taccgtgtca atctccctgt cgcggcgtga aatacttgtc aggctcctcc
    10801 cattgattga atatgtgatc cgtgcgaacc ggctgttaca cccttcccat ctttcttcct
    10861 tcacataaaa ccttcttttt tcggtattcg tactgccgtc ccacgacaga tgcgactgag
    10921 ccctaaccgg gctgaccatg cgtatcagat cggtatgctc ctcggccaga tcaagcaaag
    10981 ggcgccacga gggagcattg taaatattgt attcatcggg gtcatccggg tcaatcagaa
    11041 atcctcctat ttcatggaaa ctgacagcag gacggtcaac ggtcaatccg tttaatgttg
    11101 ctgtaaggcg ttctttcctt gtcattttac gcatcgggac aactaataat atagtaaatt
    11161 tagccaataa taagttaccg gctatcgaaa acgacaaacg gaatatgccg gaattatccg
    11221 gattgaaatc tggtttatat tttatttaat caagatcttt actttgtaca ttattttaaa
    11281 cttttgtaat ttcatagaat atgaaacccg catggtacaa aatcacaaac tagctgaaaa
    11341 taaaattcat ttgggttcca ggtgaatcaa ttaatcttca gtagtaaata attttatttc
    11401 cacatgagaa tgattaaaat cccgatcctg ttttttcttg caaatctttt ttgtgctgtc
    11461 atatcggggc aggagtatct gaaggcctca gacctggaca agcctgactt tcagtggccg
    11521 gagggaaaaa agatggccct cagcctgacg tttgacgacg cacgcctttc acaggttgac
    11581 aaaggtattc cattgctcga caggtatgat gtcaaagcta cattctatgt gtctatcagt
    11641 aacatgatcc agagactgga tgggtggaaa caggcaataa ggaacggtca tgacataggg
    11701 aaccattcac ttcttcatcc ctgcacaata aactatggct ggccgcagga ggcagctctg
    11761 gaaaactata ccctgcagag aatgacgaaa gagcttgact cagcttctgt catgataaac
    11821 aatattcttg gtatcaggcc ccattctttt gcatttccct gcgggcagac ctttgtcggc
    11881 agaggtgtca acctaaaaag ctatgtgccg cttgtggcat ccatgtttga atcaggaaga
    11941 ctctggctca gcgagggacc caacgatccg gttttctgcg atatggccca gcttacgggt
    12001 atggaacttg acggcaaatc cttcgggcag ataaaatcac taattgaatc agcatcaatt
    12061 aaaggacaat ggcttattct tgcaggacac gagatggacg aacagggccg tcagacctca
    12121 ctgcttgaaa ctatagaagc catctgcaaa tatgcatctg acccggctaa cgggatatgg
    12181 atagataatg ttcacaacat agctgcctat ataaaatcaa aaagagggca cgatgactga
    12241 gctgaactta ttttgtacaa agttttctct ttccatgtcc ggcgaaaggc agataagttc
    12301 attacgggaa tcacccggag gaaaaacggg cagataaatc tgattgactg aataggatac
    12361 gaatcggaag ggtaatatag tcaaatggtt tacagtttgt ttaacccagt gatcttttaa
    12421 aggtccgcca tacaactcta aattccggtg aattaatatt atctgttcct gcaacaacgg
    12481 atgaattatt aaaagccccg gtacagcctt gttttataat ttaatgcttc tgatcaagga
    12541 aaaatgtcaa aacatatcgg aataatcgca tgcagctatg aaggtgcagc cttatgctac
    12601 aggacaatct gcaacgaagg ctcagattac ctgggccggc attcacaccc cgaagtcagt
    12661 cttcataatc accctttaag cgaatacatg aagtatattg aaaaaggaga ctgggaaggc
    12721 gttgcctcgc ttatgatctc atcggcacgg aagctggctg gtgccggtgc aggcattctg
    12781 gtgtgccccg ataacacgat tcatcaggca tatgaaattg taaggaagga aataccggtc
    12841 ccatttcttc atattgctga agaggttgca ggtgaagctt tcaggaattt ccgctttaaa
    12901 acaggtatcc tcggaacaaa gtacctgatg gaaggaccag tatatcctga aaaactgagc
    12961 gtacttggga ttaagtcaga aataccatcc ggttctcgga gggaccgcat caacagtatc
    13021 attttcaatg agctcgttta cggaattatt gaacccggtt caaaggaata tttcatcagc
    13081 gtgataaatg aattcaggca atcgggctgc gacagcgtga tactcggatg caccgagata
    13141 ccgctgattg tagtgccgga agagtctcca ctgccagtac ttgattcaac caggatactt
    13201 gccagggcag ccctgagaaa agccattgaa taattctcgc accggaactc taaattccgc
    13261 acttaaatac tgccctcccc ttttccttat ttccggagat atctccctgt ttgctgttac
    13321 tgtccgctat acttttttat accaactttt tttttgtgtg aaaatcattg ttatttaatt
    13381 ttgatcatga ggatccggat cttaaatgga agattgagag ctttcgacca ggaacccttc
    13441 tgattcaccc tttatctccg ccttaacctc aagttgtttt acctttttac taatctaact
    13501 ttaaatcgct atgatgaaaa atcgaagatt tttatgttct atgctgagag gacagttctc
    13561 tcaacgaatt ttattcatcc tgattctggc atttttccag attccgctat ttgcccagca
    13621 gatcacggtc aggggcaggg ttgtaaattc agacgggaca ccgatccccg gtgtgaccgt
    13681 catggaaatg ggtaccgcca aaggtgtcat taccgatgct gacggaattt acagcctcac
    13741 tgtcagcccg gctgcttccc ttgtgttttc tttcatcgga tataacaagg aggaaattcc
    13801 ggttgacggg agaacgaata ttgacgttac cctgactgaa tccattacca accttgatga
    13861 gataatcgtt atcggttacg gcacccagcg aaaaagtgac cttaccgggg cggtggcttc
    13921 tgtgaagatt gacgagctca aaggccggtc gatcattaat gtggaacaga tgcttcaggg
    13981 gaccgtggca ggcgtaaata ccgtatcatc atcaggtttg ccgggaggaa acgtaaagat
    14041 caacatcagg ggtgttggca cattctacaa caccaatccc ctgtatgttg tcgacggtat
    14101 ccagacgcgg gacatctcgc tgatcaatcc caacgatatt gagtccatgg aagtacttaa
    14161 ggatgcatcc acaactgcca tttacggttc aagcggtgcc aacggggtta tccttatcac
    14221 cacaaaaaga ggtgccgaag gaagaccttc cgtggaattc aattcaaaac tcggactggc
    14281 ccaccggggc aagaaaattg ctgtcatgaa tgcccctgat tatgttgatt ttgttcttga
    14341 cctgaaagga aatgatgtta accagtcatt ccttgacaac attgactgga gaagggttaa
    14401 ccggaccgac tggcaggatg agcttttcag gccagcgttc cagcaggagt acaactttgc
    14461 cgtaagcgga ggttcgaagg atgccaggta tagcattggg gcgggatatg tggaccagga
    14521 cgggatcatc ataacccata actacaagag gtattcactg cgtgcaaaca ctgaattttc
    14581 gccgggtgaa cggtttaaag tcggtgagaa cttcaccgtt tcattttcca atgccagtga
    14641 agccattgaa ggacagggca acgggaacat gctcctgtgc gctttgagga taccacccta
    14701 tctgccggtc cttgatcccg acaataccgg gggatattcg agggtaacat ctgccaggga
    14761 tcataacgat gcctataacc ccattgccga attaaggttg cgcgataaca aaacaaattc
    14821 cctgcggata ctcggaaacc ttttcggtga acttgaaata ctcaggaacc tcaggtacag
    14881 gacaagcatt gctgtcgacc tggaaagaaa cgacgtcaac acctacacaa aggcccgtgc
    14941 caatgccgca tttgaatttc cgagcatcct ggatgaaacc tacagctatg gcaattccta
    15001 tatctatgag aatgttcttt cctatgcgaa taagttcggg ctgaatgatt ttctcgtact
    15061 tgcgggaaat actctttcaa aatcatccgg gagatactat tcccttacgg caacggattt
    15121 tacaaatgat cagatccgca taatccccgt gggaaccggg aaaattaaca acggcgcagt
    15181 cggggccaat cagtcagcca agcttggtta ctttgtcagg ctgaattact cctatgccga
    15241 caaatacctg ttccaggcga atttcagggc cgatgcttcc tataattttg cgcctgaaaa
    15301 caggtggggc tacttccctg cattttccct cggatggaaa ctgaacgagg aaaatttcat
    15361 taaggacaac ctcacattcg ttgaccagct taaactcagg gccggatggg gccagagcgg
    15421 aagcgaccta atcccgtcat acggattttc gtcatcaata agctctttca accccgacta
    15481 catctttggg tcggtcccgg ttaccggtgc agcaatagtt tcactgtgga accccctgat
    15541 gaaatgggaa acctcaacca catcaaatat cggtattgat gccgccctgt tcaaaagccg
    15601 tctccagctg accattgact atttcataaa aaatacccgg gacatcctaa tagaataccc
    15661 gatgccgtca tcaacaggta tagggctgtc aggcggatcg gaaggaagtg cctggagaaa
    15721 tgctgcctcg gttgtaaaca agggccttga gatgacggta aactacacaa aaaccagtac
    15781 cagcggcttt acctacaatt tcggcgccaa tgccacattc ctgaagaaca aggtcactga
    15841 cctcggcgac ggattgccaa taatggccgg aagctttgca ggcaatgcca tgacccggac
    15901 tgaaaaggga catccgatag gttctttcta cggatttaag atggacaagg tctattccac
    15961 acaggccgag gtggatgctg ataatgagag agcccgcagt cttcacggcc cggatgccat
    16021 gtaccaggag aatgcacagg caggtgatat ccgcttcgtg gatgtcaaca acgacggcct
    16081 gatcaccgac gctgataaaa cattcattgg atgccccatc cctaaactgg tttatggatt
    16141 caatctcggt gcatcctaca ggggatggaa cctgtcagcc aacttctcag gtgtatccgg
    16201 caaccagatc tttgacagca atcagtactg gctggaaggt atggtcattc ctttcaatgc
    16261 atcgatcgaa atcaaaaaca ggtggaaaaa ggaaggcgat atctcaagaa tacccagggc
    16321 agtgtctccc gatgccaatc agaacacaag ggtatctgac agatggatcc ataacggtaa
    16381 ttaccttaga ttaaaggtac tgagtcttgg ataccaggtt ccggccctct tattgcagaa
    16441 actgacttca aacaccgcat cgaacctgag gatctatgtg gctgcagaga acctgctgac
    16501 tatcacagga tatcgcggat ttgatcctga aatcggagaa atgggcgata acctcgtcca
    16561 gggggttgac tgggggcagt atccgcagtc aaaattatac atgtttggac ttgaagttaa
    16621 attttaattc gtagaaaaaa tgaaaagaat catatactta ttcttcggga tattattttt
    16681 cgccggatgc agtgatgatc tgttgaacct ggaaaatccc aaccagtatt ccgccgacac
    16741 ctattttaaa aacctggaag agtgccagcg aggaataaat gccatttacg gggggtttta
    16801 ccacgacggc ctgtatgcca gggaatacta ttttattttt gattgtcttg ccaacgaagc
    16861 agaacagaat tcgccgatgg aggctcctac aacagactgg cccaattata cccatcatcc
    16921 gggcaattca gagcttcagg gtctgtggag atccctttac cggatcattt tcagggcaaa
    16981 tgtagccatc gaaaatattg gcaaatggga accggttaag gaaagcgagg cccaaagaaa
    17041 ggagaccatg atcggggaag catatttcct gaaaggctgg gcatatttcg aacttgtgaa
    17101 tatctggggc agggtcccca taaggacatt tgagaacagt accgaagcaa aggtaccgcg
    17161 aagcaatacc gaagatgtat ggaaaaccgt tgaggacgac ctgaaggaag ctatcgacag
    17221 gctaccggtt tcatgggata atgataacat tggaagggca acaaagggtg ctgccacagc
    17281 tttgcttggg aaagcttacc tgtatgaaaa gaagtatgcg gcagcccacg ctgaatttgc
    17341 aaaactcgct tcagcaccct atacctatga tctgcatccc gactaccagg agaatttcct
    17401 ggaggccggg gacaacaatg aagaatcggt ttttgaggtc cagttccggg gcggggcaac
    17461 aggtgatgca gtttggtaca tgtttggcgg ccaggaaagc tggggcattg gagggctcca
    17521 cacaggcagg gcaaccgaat acggattcaa cgactgggaa aactgccacg tatcaaatgc
    17581 agctgttgca gcattcactt acaagaatga atccgggacc gattacattg atccccgtgg
    17641 tgccatgctt ttttatggcg acgcagcatc cggaggtgat acggattatg ccgggggaga
    17701 gtttccgtat gaggccaaag gctacatgtg gaaaaagtat cagacctacg aggaaaatga
    17761 atatgaaggt gacccgaaca gcggtatcaa tgcaaaggtg atccgctatg ccgacgttct
    17821 tcttatgaga gccgaagccc tgataatgga taataggatt ggtgaggcac tgcccctgat
    17881 aaacaaagtg agggcccggg ttaacgcctt tgagtatact gacctgggaa atcagtcaga
    17941 ggcaatgcag aagatcaagc tggagagaag gcttgaactg tgcggtgaac aggtgcgatg
    18001 gtttgatctg atccgctggg gagaggctat ggaagtgata aacgccgaga aattcgctgc
    18061 aaaaggtgtc aggcccttcc tgcccaagca tgtcctcttc ccgataccgc aggaggaaaa
    18121 ggatgccaac tcgctggttg cagatgatat tacgggagac tggaattaat tatcccgggt
    18181 aaccggaata atgaaacaag gctgccttaa tagccgtttt taagctataa ggcagccttt
    18241 ttattttttc agacaatcct ttttcttacc ctgaacttgc ttatccgaag ccggtttaac
    18301 ggttgctgaa ccttcttacc gggtccggct atttcttcag cttaagcaga tccacaaact
    18361 cgttcaggaa aaaggtattc tcatggatgc cggaaaacct ggcagcaccg ggtccgaatg
    18421 aaaatatcgg aaccatcaca ccggtatggc tgcccgactc aatgaattcg cctttcacca
    18481 gtttgccggg aatgcttccg cctgtgaggc tcaggccgcc agtttcatga tcggcggtga
    18541 ccacaataag ggtttcgccg tttttttcag caaacttctt tgccactcca attgcttcat
    18601 ccatatctat cagttccgac accgcatact ctaggttttt ctcatgtgcg ccccagtcga
    18661 tcattgatcc ttcaaccata aggaaaaatc cggcttcatc cctgcttaac acatctattg
    18721 cctttttcac catctcggga aggatccctt ttcttccttc agcaactgac ggcatatgct
    18781 cctttgccag aagacctgca atcttttttg actcagactt cttaaggtca gccagcgaat
    18841 aaacaacatc atagccctgc tgcctgagct tcagggtgag atcatcattg tcgttccggt
    18901 tgcggaagtg gtcctccccc cctccgatga acacattaac cgttccgttc aggaaatcct
    18961 ttgcaatgtc ttcataattt cccctgcctg aactatgtgc aacaaatgaa gcgggtgttg
    19021 catgcgtaac cgctgatgtg ctcagcactc ccgtggccag tccattgcga tgggctattt
    19081 cagttattga ggtcaccgcg gcagaatcgg gcccggtacc tatcatgccg ttatttgttt
    19141 taacaccaca ggctattgct gtacctccgg ccccggaatc ggttacatag ccattatatg
    19201 aatatgtttt tgaaaatccc gtaaaaggaa atgattcaag cacaagtggc ttttttgagg
    19261 tactcatggc ggcatagaca tgggacgttc ccataccgtc gccgataaag aggattacat
    19321 ttttcacccc tgtttcggca gcacgcgttt tatcaatttt ctggaccgaa caacccgaaa
    19381 caagcaacaa aactgcaagc aatgctggtg ataataattt ttctctcata atatggtttt
    19441 taaatttcca taaatataat gtctttgaaa aaaagaaaaa taattatttt ccacatcctc
    19501 tgagttccac ctcgagcctg aggtttgcat ctgccggtaa aaaaacagcc tgcccgctga
    19561 attccacgga tgaatctccc cggtcaggac tcgtgacttt cacgatcctg gcacccatgt
    19621 cttcaaccgg aacaggatag agcttctctc cggcaaccgg ggaggatacc atgaagtgta
    19681 aaacaaccgg ttcgcaattt acccaaaggt tcaggtaaag ataattcagc aggcaatgct
    19741 ccatgttgtg gtatgaggat ttaaatcgtc ctgccttggt gcggtcaaga acattcccgg
    19801 ctgaatctat cctgaaaaat gtatccccat gcttcctgtc gacaaatgct gaatcccata
    19861 accgggcacc gcgtatgaaa taatcaatgt atttcgcatc accggttgct ttgtacaggc
    19921 acaggcttat catattgcca taagcctgga tccaccagta agtatccgat ccgggcattg
    19981 aaggttcggt tctgccggca gtggctaacc agacattatt ttcattgaat actcccgatc
    20041 tgaggatctt atctgcaatt ttcaacccgg cattcttatt cccctcatcg ggagtcagaa
    20101 accagtaccg tagcatcatc caggctgttt caatattgtg tccggtattt atttccgaat
    20161 catcattccg tgagctgaga tagttccatt tgctgtcaaa atcttccagc acccagccat
    20221 attcggtgtc aaccattctt tttgaaacaa catccagtat tttgaaggtc tggtcaaggt
    20281 atttctgatc cctggtggcc agatacaggt agaacaggta acccgaaaca ggcgttacct
    20341 gggaactgaa tgatttgtta ctgtccaata tgctccagtc gcggttcatt atattgaaat
    20401 atcctccacc gccgtccata tcccagactc ttttctcaag caggtcgttc gatctctcaa
    20461 tgtagctgag gatcacactg tcgcgggtag caaaataata catggccagc ccggtaataa
    20521 cataaacctg gacgaaagtt gttttggtga gctcgaccgg attaccctcc tcatcaagtg
    20581 catcatacca gcctccctgc tccctgtccc atgctttctc aatgagccat gcggctgttt
    20641 ttcctgcaat ttccagatct tcttccctgc ctgaaaggag ataggcggcg gaatagctga
    20701 acaggtgtct tgagatcatg ctgggataaa tatccgttga cccgaacggt ttccataacg
    20761 aatcaagatt ggtatggaaa gttccggatc tttcattctg agaatatctt gtccaggcag
    20821 gtattatgtc gcgcatagcc tgtgttttcc agtattctcc ggaaagtatt tcgtcttttg
    20881 ccggtttttt attgttgcat gatgatatca ggagcaaaag gcaggtcgca caaaaaaagc
    20941 ttttacgggt cataatatat ctttttcaga atttttgccg ggattaatca aatcatatca
    21001 aagaaaggaa ttattcttca ctgtttaggc attttgaggt catccgatct gaaacaggat
    21061 ttttagatct tctctgccgc attgttatta gaaaatttta tttcatcttt agtccttcaa
    21121 gtaatgccat tatcatttca aatgactcac gacagccatt atgatcttgt gaggaaactc
    21181 aggcccgcca ggatcatttt gcctgtcatt atcggggtcg tggtggtggg ctggttgatg
    21241 gtcagggaga tagacaccag ggcactttct gaactcaact tcacctggaa atcggcattc
    21301 tggattggag ttgcctggtg ctgcatgata ggcagggacc tcggatacat gatcaggata
    21361 aggatactgg ctgaaaaaga tatcagctgg ctgcaggcct tcaggataat aatgctctgg
    21421 gagttcgcat ccgcaatatc tccgtcgact gtcggcggaa cagctgttgc cgtggtgttt
    21481 gtacataaag aaggcatatc tgtcggaaga agcacttcga ttgttcttgc cacatcattc
    21541 cttgatgaat tgtattttgt gatcatgttt cccgttatat tgctcgttgt gggaaatgat
    21601 atacttttca ccacttcgct tcagggcacg gggataacac tgttgaacaa cctggtgata
    21661 gtggcggtaa taggttatgc tttaattctg gcctgggtaa ttttcatcgg ttatggcctg
    21721 ttcattgatc ctgtcaaaat taagaagacg attacatatc ttttcagtct tcccttgttg
    21781 aacagatgga aaggttctgc cgagaaggcc ggaaacgaca taattgacag ttcacatgag
    21841 ttaagaagaa aatcgtttgg tttctggata aaggctttaa ccgcgacatt tctctcatgg
    21901 acatcaagat actgggttat caatgccatc ctggttgcat ttttcaccat aaacgatcat
    21961 ttacttattt ttgcgcgtca gctggtaaca tggataatga tgataatcag tccaactccg
    22021 ggcggcagcg gctttgcgga agtaattctt gggcgttata tctccgatgc cattcccggt
    22081 gatccggtca caaccggaag ccttgccctg gcaattgcaa taatctggag gattatcagt
    22141 tattatccat atctgataat aggcacttca atcctgcctg catggatcaa caggaagttt
    22201 gtaaaaccag ggcccggtgg caattgaact ctgcctgtta agacattata atattttgtt
    22261 gaaaagtgag tgctggcagt gcatgttttt cctttaaatt gtgtcgtttc aaatgtcctt
    22321 gtaccaacca atagtttgag ccggatgaaa gttaaagtgt tattgatatt gctaatagga
    22381 ttgcttctgc actattcctg cagcaacagt cctcaaaaac cggccggaag cccggctgca
    22441 gctgattcgc ctgaacacta cggatcaggc tactcctccc ccgagtcgga gaatgatttt
    22501 ccggtaaatt acttttctcc tgtcgaaata gccgatctgt ttcagaaaat cggcgtgcca
    22561 ttttcaaccg aatatctggc atcgccgatt gatcccagga ctctgacaac ctcttttgac
    22621 aaggctgtta cccttggcat tctgtgtgct gacctgggct acatgaatat gtatgaaatg
    22681 acaggttctt tcccggatct tatagcctca atcaaaaccc tggctgaaga tttaggggtg
    22741 agtcagtttt tcgatttcga tgccataaca aggctatcct tcaataagtc aaacatggac
    22801 tcgttgcttt tcctgtcgct cacctcctac tcacaggccg atcattacct gagggaaaat
    22861 gatctcggac atttgtcggt attgatgatg acgggggtct ggctggaagg tcagtatctt
    22921 actgtaaaag tttttgagaa atattccgat ccgaagctga aggacaggat aggagaacaa
    22981 aagctcttcc ttaaccatct tattgaaacc attaaaaact attgcggtaa ggacactcag
    23041 tttgaaaggc tatgcagtga tctgaggaaa ttgaatgaca gctatacgga tgtcaggatt
    23101 tcatatacca ggggtgaacc ggttgccaca aaaaagggaa agggactggc aatacaacag
    23161 actgaaacca gcaatgttga aatgtcgcag gagcagctgg cacggataat ggaagcatca
    23221 gccggtctca gaaacaatct gataaaaaaa aattaatatg aaactcacat gatacaaaat
    23281 cacaaacaag catgaaatta aattcatgtg agttcaatgt gaaccactta atcttcagca
    23341 gtatataaac ttattttcac atgaaaaaat attgcatcgg attaatcctg ttcctggctt
    23401 cagttacctg catgtatggc cagaaaaatc ccgacaagtt catcagcaac tgtattgcca
    23461 ataccgggcc cgattcaaag tatcttaagg atttcaggat ccagctcgga cagagcacaa
    23521 cctcaaatga attcaggttc aaggcaaaaa tatacctgtg gaaagatacc aagtacaggt
    23581 tcacaatgtg tacggctgag aattcaaagg gacagcttat catgaacata agggatgaac
    23641 tgaacaagct gatactcgaa tcttccgatt caaaaacagg aacaatatat ccgtttatcg
    23701 attttaactg cacccgaagc ggactctatc agatctgcta cgatttcagc ggcatgcttt
    23761 ccggatcagg cgccgggatc atatcaatac tggaatgaac cgctagatga aatgcaaaac
    23821 ctgtattagg gctgtttcaa taaaaaggat gaggaaatag atcaccggaa cggtcctctt
    23881 ttttcctgca aaaacgaaat agtgtgacat gacatagcat gccgggatgg cagcaagcca
    23941 ggatatttca accgacacgg ttcgtgagaa gttcattacg gcaatggctg tcagaaaagc
    24001 ccaggtaagc agtataaaag ttttcctcga gcgtattttc ttggtgttaa gtacggaaat
    24061 tagatcggca aaactgatta ttaggatcat gccgttaatt atcagaaccg taattgcaac
    24121 ccttgagaaa acatagatac tttctttttc aaacaggtta tatctcacca aatcgctgaa
    24181 ttcagtcaag ttttttcctg tcacataata aaagcctgat accaggaacc agggggttgc
    24241 aaggccaagc atggaaataa ctatctcttt caggttcaca ggacggagct gggctatgcc
    24301 gataaacagc agtattacga accaaatgaa atttgcataa aagaggctcc cgatgctcat
    24361 gagaaaacct gcatcataca aaatgtaggc agtgccctga attttgtagg agtcaattat
    24421 tttcctcagc gccattatga aaaaaactgc agcaggtaaa accggattca tctgttgctg
    24481 ctcgggaaac aggccgctga agaggatata aatcaatgcg ggaagaaagg ttcgttcccc
    24541 tataaaaaag acggatgtat tgaaattcac aagcaggaag gctgtaagaa gcaggagcag
    24601 gaaagcagca acaacgttaa ctgtcggatt aaaaccaatt acggaaagca acagattgta
    24661 gagaggcatg ggtctgaggc tgaaatccgg agccggctgc ggcaccggat gcagaaacgc
    24721 acccatccaa accagtatgg cgacgagcac aataagaatg attacatccg gtgttactcc
    24781 cctgaattta ttcagcatta ttgttcagat caaatccgat atcgtttctg cagtatttac
    24841 cagtgaatga tataagggct gcatttctat agcataggtc aagcgcctcc cccattgaat
    24901 tgccccaggc actgactgaa agcacccggc cgcctgaggt tattatttta tcttcttcat
    24961 ttttcgtacc tgcatgaaat atcacgcaac ccgaagttct gtccagaccc tcgattttat
    25021 atcccttccc gaagctgccc ggataacctc cggagacaag cattaccgtg gcggcaaatc
    25081 tctcgtccgt aaggaattcc ctttcgccga gattgccctg ggcaatacct tccatgagct
    25141 caaaaagatc cgaccttatc ctcgggatca cggcctcaga ctcaggatcg cccagccttg
    25201 cattatattc aattacataa ggatcgccgt caacatttat cagtccgaaa aaaataaatc
    25261 ccttgtattc gatcccctcc tcaataaggc cgtttacggt cggatcaatt attctggtcc
    25321 tgactttttc catgaaaacc tttccagcga agggtaccgg cgaaacggcg cccattccac
    25381 ccgtattcaa tcccttgtcg ccttcgccta tcctcttgta atcctttgct tcgggtaaaa
    25441 tcttatagct tctcccgtcg gttgcaataa acaccgaaag ttcggttccc ctgaggaact
    25501 gctcaatgac aatcctgttt ccggcatttc cgaatttacc tccgaggatt gccctgatct
    25561 cattttcagc ttcagcaata tcgtcaataa taagaacccc ctttcctgct gcaagtccat
    25621 cagccttgat cacatatgga ggatccatgg ttcccaggaa acttattgct tctttttccg
    25681 atgattttcc gaaactctgg tacctggaag tcggaatatt gtgtcttgta aggaaggcct
    25741 ttgcaaaatc cttgctgcct tcaagcatgg ccgccttttt aacaggcccg atcacaggga
    25801 ccgtacgcag aatatcatct tcgaggaaga aatcatgaat acccgatacc agcggtgcct
    25861 ccggaccaac aacgaccatc cctatcccat tctcaataac agcctgcctt accccggcaa
    25921 aattatccgg actaatatcc aggtttgtcc cgcattccgc ggtccctgcg ttccccggag
    25981 caataaagac ccggctcacc ttcctgctgc ctgacagttt ccaggccagt gcatgttccc
    26041 ttccccccga tccaagaatc aataaattca taatctgaaa ttaaaaacta tactggttaa
    26101 gaattatccg tcatcctgct gtattttgag aatgtctgca tcacattatc cacatacgtt
    26161 tttgaaaccg ggatatcggg caccggcgat atttccagct ccagcttcaa accatccttc
    26221 tccttcctga taaccctgac cttctcaaaa ttaaccatat atgacctgtg gcacctgatt
    26281 atcccggtgt cagtgaactt ttcttccatt ttcttcaaag tgtcacgtaa aaggtatctt
    26341 gcaatttttc ccttgttgag ataaaatatg ttcacgtaat tttctgctgc ctcaatatag
    26401 aggaggtttt cctttttcac cgaaaacctg agtattgatt tctcgtcgta aaatggtatc
    26461 atgtcccttg tgccatctgg aactgccggc atgtccgaca ttctctctat ctgctggttc
    26521 ttatcacgcc acgagagata aagccacatc accgaatatg gcaacaacaa taccagcgcg
    26581 gtgtttctgg ccgaaagttt tacaagaaag acaaaatcac gtgaatcttc aagcacgaat
    26641 ttctcaaaca aggcataaaa caaagccatc agcattattt cccccgcaat ccaggcaaga
    26701 tactgccata gccggatgat gtttttcctt cccctgtaat acatgatcac cctgcttatc
    26761 accacaataa gcacaccggt aagtatcacc aggcttgagt aggtgaaaaa ttcgggcctg
    26821 gtaaggtcaa accacctgtc ggctccgaac ggaaaataaa tatttatgaa taccagggca
    26881 aacaatgacg tgaaaaggat aagccttaca atattcgact tttcattcag ataagatggt
    26941 atcggcttct gcagatcgat cattgattta ggaatagcac tcacaaaatt aaccttttta
    27001 tgacatcagg gcccgacttt ggaccggctt tcagaaccag ccccgtaaat tttcatccct
    27061 catttctaat ttacgtcccg taatcggtct ttatgtcccg taccgatgcc gcctgcacca
    27121 tttttttgcc agcaaggtca gggcgggttt cctttgcagt aagaatcaaa acagtctggc
    27181 caaatggatt acattacaag tttctccata ctgaagtcgt tatacagcat taacctgaaa
    27241 gaagtgacaa tacccggatc ggaactgaaa atactcaata ttgaattcag gtccgaatcc
    27301 aatccggaag gtttgatgaa tgacgaagat ctatcaataa cggaaaacaa tttcttcaca
    27361 tatccggtat ttgttccgcc gggcagggtg tcggggaagg tggtacttct gcttcacgga
    27421 ttgaatgaaa ggaactggat aaaatacctg ccgtgggcat accatctctc agtcaataca
    27481 ggcagctatg tcatattgtt tccgatctca tttcacataa accgctcgcc ttcgtcatgg
    27541 atcaatccca ggctcatgac gggtcctgta aaggaacgga atgacaaatg cagtgatgtc
    27601 cggatgtcga cctatgccaa cattgccctc agcagcaggc tttctcagga tccgatgaga
    27661 ttttttcatt ccggctgtca gactgcattt gacatcataa agctggttca acaaatcagg
    27721 tccgggaatc acgaagtgat cccggcagga tcgggtatta atatttttgc ctactctatc
    27781 ggagcctttc ttgcgcagat ccttgttatg ggtaatcctg aaaatctttt caccgattcg
    27841 aagcttttca tgttctgcgg cggctcggtt ttcagctcga tgaagggaac ttccaggcta
    27901 ataatggaca gtgaggcatt taacaggatc tataatttct atcttgatga ctttgaaaat
    27961 gagatccgcc gtaaaagctc aaaatacggc agtataagcg aaagccagct tggaatggca
    28021 ttcaggtcaa tgatcgattt tggaaggttc agatcattca gggacaatct attcgaaaag
    28081 ctgcgaggcc ggatcaggtc tgttgcattg tcaagggatt cggtgattcc ggcagaaggt
    28141 atcgtcagga caattttaaa aaacaggaaa cccggcagca ttgtcgaggt ctgggatttt
    28201 ccctttgagt attcccacga gaatcctttc cccgttcata tccggtccga tgaaaggtca
    28261 gtaaattcct ggttcagcag gttgttttcc gaagcatgcc tgttcctggc atagcatgat
    28321 caatcgttac aaaacgataa ccctcaccgg aggcgtattc aatgaattcc gcaagaaatc
    28381 ctgtgacttt tgaatccggg ctgtcatgca aaacaatgat agagccgggt ctgatccttt
    28441 tcctgagtat actcaggcac ttagcggctg actgagatcc ggaaaagtca taaggcatga
    28501 gatcccagaa taccactatg tattcacgga tcagctgatt atactgcctt aaggtcagcc
    28561 tcccataagg aggacggtaa aagcgtgagg gaaccggccc ggctgcccgt gatacattgt
    28621 ctgcatacct cgaagacgat gttttccatc cgctgatatg ccggtaacca tgattaccgg
    28681 tcatgtgtcc cgcggcagtt atcatatctt ttaccccggg atatttcacg gcattggcac
    28741 ccgtaagaaa aaacgttgcc ttaacctggt atttatccag gatatccagt atcgcaacgg
    28801 ttgaatcagg gtggggtccg tcatcgaaag taaggcaaag cagcttttca tcggtcggta
    28861 tccggaaaat tgctttcgta aaaatctttc tgatcagaaa aaaaggcctg tattgcctca
    28921 tcggtatcag tggttcgaat aaagcaggtt gtagaagctg ctgacttcag gttcgatttt
    28981 ttctaatagc ggatccagct tgagcttaac tgccatattg taaagatcga ggtacgactg
    29041 catgttaatg cctgttgcat aatccatccc gaaccggagt tcaggtttga ggtttactga
    29101 gtactcaagg tattccctcg aataattcag aatatcatca ataacctttt cagcttcttc
    29161 tttccttcct gccttaagaa gtacttcggc cagcgtaacc gagaaataat cgttcggcat
    29221 cttggaagcc gggactatct ccagtcccct ttttacagct tcaactgccc tgagcgtatc
    29281 acccgaggaa agcagggcgt ttccgaggct tccgaaaatc ctcctgaaat tgcttagcat
    29341 ccgcctgtta ttctcatcga ggtaaacacc gggatccgcg gcattgcccc atccgaattt
    29401 gttcatcata ttgtcgtaca tcacaaccgg atctatcatt ccaaactcac ccgcttcagg
    29461 ttttgtcgta cgtatcggca caatcctgta tgccattcct tcctggacga agaacttctc
    29521 aagtcccttg tactggctgg aaggaacggt agtcgacatg tatatcggcc ttgcccattt
    29581 gtttgttgcc agaaggtcca taatggccag gtcgcccttg aatgcatctt cccttgtgta
    29641 ttcccatatc attggtgata caatcctgtc cctgtaatac tccttgaccg tgccatttgc
    29701 cagaaccacc tcagggtcaa cgtctataat gaacttgttt gcaggcaggt aattcagata
    29761 attgcctcgt ccgtttatgt ctatcatgta tttcctgtcg tcctggattg caaactgaat
    29821 tatctggcta agctccaccg gcttttcaag cctgctgttt accggaagct gatttctcac
    29881 tccttcaatg tacttctctg ccggtatgga aagcggcatg ggatccgaat cgaatgcctt
    29941 ctgcctcatc atgtcgatgt accagcccgc ctggagatag ctgagattgg ccacacggat
    30001 atctgttctc acaccttcaa catcctgaat ataccagacc ggaaaggaat cattgtcgcc
    30061 gtatgtaaac aggatactgt ttggtgcaca tgatttcagg taattcgcac cgatatccct
    30121 tgccgtatag cgccccgaac gatcgtgatc gtcccagttt tccactgcca taagaacagg
    30181 cactgcagca agcaatatga taaaggcaat agcagctgac aggtactccc tcagaaattt
    30241 cttaagcaat gaataaacgg gcatgaatcc tagccctatc catattgaaa acgcatagaa
    30301 cgagcccgca taggcataat ctctctccct gggctggttg ggatactgat tcagatagaa
    30361 aattatcgct atccctgtca taaaaaagaa agccatcaca agccaaaaat cattcacatt
    30421 gttcctgtaa tgccacaaca aaccaacaat gcccagcaat aatggaagaa aataataagt
    30481 gttcctgccc ggattatttt tcagatcttc gggcaggtta tccagatccc cgagtcttgc
    30541 cttatcaata aagtcaatgc cgcttatcca gttcccgtga attggatttc cgttaccctg
    30601 gaaatcattc tgccttccca caaagttcca catgaaatat ctcatgtaca tgaacccgac
    30661 ctggtacctg aaaaaatacc ggaggttctc agcgaaagta gggcatataa gagtctcatt
    30721 cccggaacct gagcttacca cgtaccttct gcccgtgatc tttccccagt atttatatgc
    30781 attttcatgc tcgggatcag ggctgtacat acgcgggaac actgtcataa attctttttt
    30841 atactcataa tcaggcctga agtatggctt gtatttcccg tcaattttat tgtatcctgc
    30901 tattttgttc tttacatcta tgacgggcgc gctgtagtaa tttccataca gtttcgggga
    30961 actgccgtac tgctccatat ttatgtaata cgacagggaa aaaatatccg agggattatt
    31021 ctggttcatt ggcggccgtg cagctgaccg gatcattatc atggcatacg atgagtaacc
    31081 tatcattata actgccaaca ctgtcaatac atggttaagt accgttttct cccgttttat
    31141 tgaataccag atcccgtaaa aaaggactgc aaagatcagg aataccgcaa agagaaggcc
    31201 gctgttgtac gggagcccaa gcacgttgac aaaaagaagt tcaaaccatc ccgccacctt
    31261 cggaacaccg gggataagga tgaaaaccat aagataaagc actgctgctg caatgagaaa
    31321 agtctttatc ataccggccc gggttacctc atacttcctg aagtaataaa caaatatcag
    31381 gaccggtatt accagaaggt taagccggtg aatacccagc cccagtccca tgatataggc
    31441 agtaaggatt atccaccggc tggctgtggg ctggtcagct tcctcctccc atttcagcat
    31501 tgcccagaat acaaggcctg tcacgagtga cgacagggca tatagttctc cttcaacagc
    31561 cgagaaccag aaggtatcgg aaaacgtata ggccattgcc ccgataacac cactggcaat
    31621 aacagcaggt atctgtttct tttccacctc gttctccttc ccctgaattt tagctgccag
    31681 atgagttatc gtccagaaca ggaacatgat tgcaaatccg ctgcttattg ccgaaaaggc
    31741 attcatcatc agtgcaacct tcgaagtgtc gccaccggca aaaagagtgg caattcttcc
    31801 gatcatcagg aacagtggcg ccccgggggg atgaccaacc tgaagccgga aggctgacag
    31861 tatgaactcg ccgcaatccc agaaactcac ggtgggttcc attgtcagga gataaatcat
    31921 cgatgagaat agaaaaatga tccatccggt tatcatgttt atccggcgga aactgcccca
    31981 tactgaattc atgtcttccc ggttaaatta tcaaaagaca aataacggaa aaaaaccggc
    32041 atctgcgacc acaaaggaaa ctattaaact atatttaacg cagctttaat tttatacttt
    32101 tgtttcaaag agcccggtta atgagaaaat acggagtttt catcctggtg ctgatcttcg
    32161 gcctgcagca gcttcactgc atgaactctc cggatacctc ctctgcagga agcctcggca
    32221 tgagattccg gaatatcagt ttcgtaagga acaatgaata ttcaaatccc attaccgaag
    32281 gttataccct gatcggatat ttcattcagc cggaactcgt ttactccccg tcagacaggt
    32341 taagactgag cctgggaata catctgctgc agttttcggg caccaacggg ttccacctgg
    32401 tcaaaccggt ctttacatct tcattcaatc tcaccgggaa catggttttc accatgggca
    32461 ccctgcaggg aagcgactca cacaggatgt tcgaccctca tttcagcaag gaacggctct
    32521 acaatgcatt tgctgaagac ggacttgaac taaggttctt aaatgatcat cttttcaatg
    32581 acgtgtggct cagctgggaa aattttattt tcaggggcga taatgagcgt gaaattttta
    32641 cggcaggtga atcgttcagc tacagttctg aaaaaatcgg aagatcaatc aggatcagga
    32701 tcccggttca gatgatgttt aaacattacg gcggccagat aagcgattat ccggaaaagg
    32761 ttgaaaccta ttttaatctt gcggccggtg ccggactgag ctacgatata gctgacaggg
    32821 attttggtga aacaggtatt gaggccgtat tttattatgg gaaatcacta acgggcaaac
    32881 cgtcttcggg aatcggcagg ggcaccggcg cctggtacaa gatccgttat tcatacaggg
    32941 attcggaaat tgatgccggt ctttggacat ctgatgattt ctttgcaccc aacggcaatt
    33001 atatcttcgg ttcggtctcg gatcactttc ataattatgt cattcacagg agaaggctgc
    33061 ttacctgttc ggcaagtatc agactggtgc ataaatcatt cctggaattt ttccttggat
    33121 ttgaaggatt ctgggacact gacctcagga ggtttgataa tgccgtttcg cttcacctga
    33181 gatttgataa gctgatcagt ctggccaggt taaaggatca gaggtaatag cgccacggcc
    33241 ttgttttcca gtattcacct gcatagtcaa taccgatcct ttcagaggtt ttgtaaccgg
    33301 tggcaatgcc gctgtcctca atccatatcc ttgcagatga aaccagatcc tctccgttaa
    33361 atgacctgtc aatacccagg gccctggtga cccgtcccgg tcctgtgaaa tctttcacac
    33421 ttcttatcag ggcagcctga ggattttcac ttggaccggt gacgatgttc agcatccagt
    33481 gaatcccgta tacaagatat acataaagcc tgccgccgtc gtgatacatt acttcagtac
    33541 ggttcgttcg gcctttgctc gcatgacagg ccttgtcctc cggacccctg tatgcttcgg
    33601 tttcagtaat gacatacctt gccattgcac ccttgccgtc ggccattacc agggttttta
    33661 aaagaagctc aggagcaacc tcaaggacat ctcttaaata aaaatccctg ccaagtcttt
    33721 tattggaagc catcagtcta ataaacgatt ttgctttcca tgtcattcca gagtttaaaa
    33781 ggctcaccgg caccctgaac atgcacttgc cggaacgaaa ccgacctttc cgcctgatcc
    33841 atgcagatct ccccgtatat atccctgtca tcgaatcctg cagctgccgc atcattcctg
    33901 tcggaagagt aaaccactct tcttatccct gaccagtaaa ttgcagccag gcacatggga
    33961 cagggttcac atgatgcata gaggacacat ccggtaagtt tgtgagttcc aagggccttt
    34021 gcagctttgc gtattgcaag tacttcagca tgtgctgtag gatctttgag aagaactacc
    34081 ctgttgcccg cttctgcgac cagtctgtca tcacgtgtaa tcaccgcccc gaagggtcct
    34141 ccccctgatc gtacagattc tgtggccagc gtgattgctc tttcaataaa actcctgtca
    34201 atatccatta ggaaacagtt tattcctttt taattcaacc ccgggaaaag ccgtttgact
    34261 attgctcaag aacccgggta taattgtcat gcgctgatga accgccgggt tccgcggtgc
    34321 aggaaaacat aatgattttc cctgaatcct ggccgggaat ccctggaaat aacggtcaaa
    34381 tatagaaaat ccacaaattg atacccgtta ataatttatt ttaaatcctt ttttatcagg
    34441 aaaaatattg tacatttgca gtcccaaaat aacaggtttt atatctattt tttgcgtttt
    34501 caaattaaaa ttcattaaag tgaatacctt aagttataag acagtttcgg caaacaaggc
    34561 taccgtaaac aaggagtggg tgctgatcga tgcggaaggt gcggttcttg gcaggctggc
    34621 aacagttgtc gcttcaaccc tcaggggcaa gcataagaca aattttactc cccatgttga
    34681 ttgcggtgac aatgtaattg ttatcaatgc agacaaggtc agtctttccg gtaccaaatg
    34741 gtccgataag gaatacgtca ggcacacagg ctatccgggg gggcagaagt tcaccacggc
    34801 cactgagatg ctcagaaaaa atcccatcag cctggtcgaa catgcagtga ggggaatgct
    34861 tccgaaaaac aggcttggaa gggcaatttt caagaacatg catgtttatg caggagcctc
    34921 ccatccgcat gaggcgcaaa aaccgaagat catggaactt taacccaatc aaggaacaat
    34981 aatggagtca gtaaatgcaa ttggaaggag aaaagcagcc gttgccaggg tctatgtaag
    35041 cgaaggcaag ggaaatataa cgatcaacaa cagggattac agggaatatt tcggtgccga
    35101 cacacttcag tatgttgtga cgcaaccgct tatggtgctg aatgcatcgg acaaatacga
    35161 tatccgtgtc aaccttgagg gaggcggcgt caaaggtcag gctgaagctc tcaggctggg
    35221 aatagcaagg gcactcatac tgattgatcc ggaaaacaaa ccggtgctca aggcaaacgg
    35281 attcgttacc cgcgatcccc gtgaagttga aagaaaaaag cccggcagac cgaaagcacg
    35341 gaaaagattc cagttcagca aacgttagcc gcaaaattat aatcatagat gcaggtttag
    35401 tatctaaatc accaggactc ctgaataacg ggactactta gtgattgact gaaaaagaac
    35461 gtaaacttta aaaaaaagaa aatgccaaga acaaatttcc aggaactgtt agatgccggt
    35521 gttcatttcg ggcacctgaa aagaaaatgg aatcccgcaa tggctcccta catctttatg
    35581 gagcgcaacg ggatccacat tattgacctt gaaaaaacca tagttaaact cgatgaagca
    35641 gcttccgcaa tgaagcacat tgcccggtca ggaaaaaaga tcctgttcgt ggcaaccaaa
    35701 aagcaggcaa aggaaattgt tgcagagcgg gttaagcagg ttaatatgcc atacgtaacc
    35761 gaaagatggc ccggtgggat gctgacaaat ttcccgacca tcaggaaggc agtgaaaaag
    35821 atgggatcaa tagataaaat ggcttctgac ggtacttttt cgaatctttc gaaacgtgaa
    35881 aagctccagg tcaccaggca gagagcaaaa ctggagaaaa accttggcag catcgttgat
    35941 ctgacccgcc tcccttcggc cctttttgtg gtggatgtat gcaaggagca catcgccgtt
    36001 aaggaagcaa aacgacttgg aatcccggtc tttgcaatgg ttgatacaaa ctccgatcca
    36061 acggaaatag aattcccgat accggcaaat gatgacgctt caaaatcaat atccctgatt
    36121 gtcaacctgc tttgccaggc aattgaagaa ggtttgaatg aaagaaagct ggaaaaggac
    36181 aaggaacccg ttcagaaaga cagaaaggtt atcaggaaag atctggaaac aaaagatctg
    36241 cctgaaaccg tgatcgccgc cctgtccgat gaggaagatg acgacgaacc cgaaactgaa
    36301 gaagaaaccg acgatgagct ggaaactgac gacgagacaa cggttgaagc ggatgaaacg
    36361 gatgaagaat aaaaactcat gatatctaaa caataaaagg aaccgaatat ggctacaata
    36421 aatgcagcag atgttgcaaa gctgagaaga gttacactgg ccggaatgat ggattgcaaa
    36481 aaagccctgg aagaatcaaa tggcgatttt gacaaggctg ttgaaataat aaggaaaaaa
    36541 ggacaggccg tcgccagcaa aagggctgac cgtgacgcca ccgagggtgt ggtactggcc
    36601 aggacctctg ctgatggcaa atttggcgca atgattgtgc tcaactgtga aactgatttt
    36661 gttgcaaaaa atgccgagtt catttcactt tcagataaaa taatagatac tgcactggtc
    36721 agcaagcccg aaaatcttga agaactgaaa gccctgaaga tcgggaatat aacagttgcg
    36781 gaaaaggtcc tggaattcct tgggataatc ggtgaaaaac tcgaactttc actctattca
    36841 aagattgaag cacctcatgt tcaggcttat gttcatcccg ggaaccggct ggcaaccctt
    36901 gtcggctttt cacgggaagg acttgatctg caggtttata aggacgtggc aatgcaggtg
    36961 gccgcaatga acccggtgtc gatcagcaag gatgatgttc ctgaaaacat tatcgcccag
    37021 gaaatagaaa taggcaagga acaggctaga cgtgacggga aacctgaaaa catgcttgag
    37081 acaattgctc agggaaaact gggcaaattc ttcagggaaa acacacttct tggtcaggaa
    37141 tttattaagg acaataaaat aaccgtcagt cagtatcttc agtcagttga taaaactctt
    37201 acggtaacag cgttcaagcg tttttccctt aatctgtaag agtaagttca taattcaaaa
    37261 acaaggaggc tgtttcagat catatgaaac agcctctttt ttgtcatgat caattttcac
    37321 gaatgttctg ataagaatgg gcagggaaat gccaatggaa caacatacaa aaaaagaaaa
    37381 gccgcttcta tcgaaactgt cttttttata gcgggatcaa tttatttcat aaatttgtta
    37441 aaagaacttg tgtttaacct aatacgtatg ccatgaagac gaatctgggt aataccgaca
    37501 agattatcag aacaatcatc gccgcccttt ttgctattct gtattttaca ggtgtggtta
    37561 agggagtaat tggtttaata ttgcttatcc tggctgtcat cctgctgctg accagcataa
    37621 taggattttg tccgctttat actctcctgg ggctttctac caaagggaaa gaacccgaaa
    37681 agaaatgatt gtcctgaaac ccctgttaaa ctcaaataca tctttaagga gtattctcca
    37741 tactacagga tagccattaa ctggggaaat tgaaaccaat ttgttatttt cgctgcctga
    37801 acctgaactg cctggcgata gatttactgc ggcaggctta aaaaaaccat gcaaaatgaa
    37861 atattccagg atcctgctta aactgagcgg cgaatcattg gccggagaag aaaatcatgg
    37921 cctgagtgac gtcatgctgg aagaatatgc atcacagatc atcgaagtcg ggaaaaaagg
    37981 atgtcaggtt gcaattgttg ttggaggcgg aaatattttc cgtggcctga gcggaaccag
    38041 atccggatac gacagggtaa aaggtgatca gatgggaatg cttgcaacgg tgataaacag
    38101 tctggccctt gaaagcgcta taacaaaact gggaggaaag gcaaaagttt tcacgtcggt
    38161 aagaatggaa ccagtgggtg aattttacaa cagggacaaa gtggttgcct gcctgaatga
    38221 taacattgtt tgcatccttt cgggaggcac cggcaatcct ttttttacga ccgataccgc
    38281 atcagccctc agggccgtcg agatcggggc cgatgtgctg cttaagggca ccagggttga
    38341 cggtgtttac aataccgatc ctgaaaaaga caaaacagca gtgagatttg aacggatttc
    38401 atttgacgaa gccatagaca ggcagcttaa agtaatggac tccactgcat ttaccatgtg
    38461 cagggagaat aatatgcctg ttgtggtttt caatatgaat gttaggggta atctgatgaa
    38521 ggcagtcagt ggcgataata tcggaacact tgtatgtaat tagccgtatg gttaatctca
    38581 gactaatttt taattttgtt ttcccggaag aaaaacggat atcataagat caaatacagg
    38641 attaaggacg tttacctaat taatctaagc aacatgaacg aagaagtaga aatgataatt
    38701 gaagaaacca gggagagaat gcaaaaagcc cttgatcatc tggagcatga attggcaagg
    38761 cttcgtgccg gaagagcgac tcccgtactc ctcgacggaa taacagtgga ctattacggg
    38821 gtaaactccc ctctttccca ggtttcaaac atcaatactc ccgaccctaa aaccattcta
    38881 atacaaccct gggaaaagac tatgcttggc gctattgaga aagccattat ggctgcgaat
    38941 attggcctta ccccaatcaa caacggggaa atcatcagga tcaacattcc tccccttact
    39001 gaagagagga ggcatcagct tgtaaaacaa gtgaggaccg aaggcgaaac ggcaaaaata
    39061 agcataagga acgcgcgtaa atgggccaac gatgaactga aaagaatgct cagggatggg
    39121 ctggctgaag atattgaaaa gaacgctgct gaaaccgtcc aggaactgac acatgaattc
    39181 tccggcaaaa tcgacaaggt gatggctctt aaggaaaagg atgtgatgac agtatagctt
    39241 caggtcttaa ctcccttcaa accctcggta caattcctgc acatatctat cccgctcctg
    39301 ttattcagta tccggtgccg gaacttatca aactcagcac catgccagat agccctgaag
    39361 gaggtttttt tcaggtcacc cataacatga tccgcattct tgtcaaaaca acatggaaga
    39421 actttcccgt cccaggtaac aaccggatta aaccatattc ttctgcaatt atccggtaaa
    39481 gtgcttttta tgatgtattt tccgtccccc ttctcataac gcctgaattt ttcaatttcc
    39541 ggcatccagc cctgaatgct ttcctcattg ataacctgca tcgattttaa tctcagccgt
    39601 attccccttt cttttgcaaa ctgccttacc tgcccaatct ggtgctcatt gtaccggttt
    39661 accaggaact ggatctcaat agtcattgat gaattcagct tctttctcgc ggctacgaca
    39721 ttgttcaccc cggcaattac cctgtctaat tcgccatttt tcctgtaact cgcatatgca
    39781 gcctggtcca ttccgtcaac agagacaata agccttccca gacctgaccc tgccaggtag
    39841 tcggaatttt cagcagtcag ataatgtcca ttggtggata cggtcgtcct gattcccctg
    39901 gctgactcga gaaagctgaa aaacagtgga tgcagcattg gctcaccctg gaaataaaga
    39961 ttgatgttac acaggtatgg tttcatttca gccagaatat cacggaacag gccgatatcc
    40021 ataaatcctc tctttctacc cataattccg gaacctgaag aacattcagg acaattaagg
    40081 ttgcaatggt ttgtaagttc ggttgaaatg gctgccggca ttccccacac ccccggcttt
    40141 cgtgaaactg agctttgtag gtatgagtaa ccggataaaa gaagattgac cggtttaaca
    40201 aaactaactg ccatgtgttg aattgaaaag gtgaaggtaa aaaaaatact gccatgccat
    40261 agggtaaaag ttaatttcac ggtctttaag taagaaagga agcattacgg tgatgacttt
    40321 aaataaaatg acttaactta ataattttat accatgaaaa cattcagaag tttatctttt
    40381 ctgccgttga tcattatatc cctccttgcc ggacagacat taaatgccgg tgttcatccg
    40441 ggaaacggga aaaatgacca gcaaaccgtc cttactttca ggggaaaggt tgtggacggt
    40501 gaaacaggta cacctttggt ctttgcctca gttacggtaa aggagacaaa tgttgccacc
    40561 attaccaata ttgatggtga attcgttatt aagatcccgg aatccgatgc ctcaaaaaat
    40621 ctggaaatta cctttctcgg ataccggaac aaaatcattt ccataagcga actgcgtgaa
    40681 aacggacgga agaatacaat ctccctgtca ccggcaccga ttccaattaa ggaaataatt
    40741 gtccggccgc ttgaccctga ggaaattgta aggaacgcca tttacaggat cagcagaaac
    40801 taccccaatg aaccaaacct gatgacagcc ttttacaggg aaactatacg caagaacaaa
    40861 acctatgttt caatcggaga ggcagtggtt gagattttta aggcccccta ctcaaacgat
    40921 cttcgttttg atggcgccag gatctataag ggcaggaaag gaacggatgt aagcaggatg
    40981 gatactctcc tgttcaaact tcagggtggt cctgtaaata ccctgcagct tgatattgtt
    41041 aagaacactg aaacagttct cacccttgag gcaatgaaaa actatgatta ctccattgca
    41101 ggggtgatcg agattgatga aaaaccgcat tatattatac agttcatgca aaaaccctat
    41161 gttgaaaatc cgcttttcat ggggaatttt tatattgagg ctgacagcta tgcactgact
    41221 gaagctgaat ttggtttcaa cctccggaac atagaagctg ccaggtctat ttttattaaa
    41281 aagaaacctc tgggggttga ggttacccct gaagttgcca cctacaggac aagataccgt
    41341 gagcaggacg ggaaatggta ttttgcatac tcaagggcag aggtaaaatt caaggtgaac
    41401 tggaaaaaga aactcttcaa tacttactat acaaccatgt cggagattgc cataaccgac
    41461 cgtaccactg aagaagtaat aaaaattgca ggaaaggaga agataaggcc ttctgatgtt
    41521 ttctccgaac aggttgaggc ctttaccgac ccagaattct ggggtgacta taacgtaatt
    41581 gagccggatc agagcattga ggcagccatc cgccgtctga gcaggaagct caggttcagc
    41641 gacaggaagg attaaccggt aatcctgttc cttcaaatga tcagggattc tgaaataata
    41701 ggcagaatta gaaaaggtga tgtaagccag tttgaatcac ttttcagatc ctgttatgtt
    41761 tccctcgtga aatacgcaaa gacaattata aaggaccatg atactgcaga ggaaatagtt
    41821 caggatcttt ttttcaggct ttggaaagaa agggaaaacc tgtctgtaaa gatttccctg
    41881 aatggatacc ttttcagggc tgttcacaac aggtgcattc atcatattga acatgaaaag
    41941 gttgtgggta aatatgccga agaaatgctg cattcaggga gcaacagccc cgaaatcgct
    42001 tccgatatac tgatatacaa tgatctgcaa acaactgttg ccaggatcct tgaaagactt
    42061 ccctggaaaa ccggaaggat tttttgcatg agcaggttcg aagggcttaa atattcagag
    42121 attgctgaaa agctgtcagt ttcggtcaaa acggtcgaat caaatatggg aaaagctctc
    42181 agggaattcc ggaaaatcct cgcagaacaa tgaaaacaaa atacgaaatg ctgaaataca
    42241 ctgataaaga atgggcagaa ctggcttcca tgctctcgga tgaaaaaaca ggacaaggtg
    42301 aacttctcag ccggttcgta tctgatgaca aatccggcgt aattaaaaac tggaaagaaa
    42361 tgcaaaagtt tagcaatgat aaggaaataa atgttgaccg ggcctggaat aatctttatg
    42421 gaaggctgag tgaggataaa ctaattggga aacctgttac cggaaagaaa ctaattgccg
    42481 gtaatgcctt tttaagaatg gcagcaggct ttctgatcct tgtcagtctc ggtgttgcat
    42541 cttacctgat tttcaaccat cgcaccaccg attccgcaat tgtcgttacc acggaaatga
    42601 atcagaaaaa cctccaggtt acactccccg atggcagtac tgtctgcctt aacagaaata
    42661 ccagtgttgc ttacggtcat aatttcggaa aaaccgacag gaaaataacc ctgtcaggag
    42721 aagcattctt caatatagtt cccgttcccg acgtaccttt tgttgttgat gccggcaaag
    42781 ctgccgttat ggcaaagggg acatcattca atgttatatc cagtaattca gccaatgcgg
    42841 tggaggttta tgtactgacc gggaaagtgg ttctgtccga caattcggga aacaggagca
    42901 tcgaactgga gcccggattt attggcacaa tgaaaaagga aacatcatcg aaaaccctga
    42961 acaacgaccc gaactatctt gcctggaaca ccgggatact tgtatatgac gggcaaacac
    43021 ttgaaaccgt tttcaatgac ttaaggaaag tgtataatat ggaaatagtt gctgatgatc
    43081 cgggaatcct gcagaaaaca tggacatcac cgataaataa tcagcctcag gaaactatta
    43141 tcaggttaat ttgtacgagt tttaatctta attttacaaa agttggaaat acatatcatc
    43201 tttctgccgt taaataaacc cctaaaagaa gaatgtcagc aggcagtggc tacatatcta
    43261 aaaatatccc ggccattttt ctgatccttt ttctcattcc acaggaaatc acgggccaga
    43321 atgcaattct cgattcaatt tttaccttta atgcaggaaa aatcaaaacc ggcgatgcac
    43381 tggataccat ttcacggcaa accggatata gtttcaccta cgacagccgt ctgatcgaca
    43441 ccgaaaagcc aactgacctg aacttttccg ggatcagcct gaaatccgtt cttaaatcaa
    43501 tttttcagaa tgactccctc aattacacaa taattgataa attcataatc atctcaaggg
    43561 gaattgagaa aaaaataagc gatcgcgatt caacagtaat atctgaaatc agatcggtca
    43621 ccgggctgat ccttgatgct gagtcgggag atccgatgcc ttttgcaaca atcggactga
    43681 aaaatgaagg caggggtacc gtttccaaca ataacgggga atttagcctt aaaatagctc
    43741 cggcctatct cagggataca atcctgatct catatcttgg ttaccttggt aaagaaatac
    43801 cagtggcaga agctcttaca aacaacctca gaataatgat gatacgggaa ttcatttcga
    43861 tacccgagat catcatcagg aatcagattc cccaggaagt gattaacagg gcattaaatc
    43921 tcataccaaa aaattatggc acaagcccgg ccctgctgac cggtttttac agggaaggag
    43981 ttcagaaaag gaagaaactt caatcatact cggaggcgat aataaggatt tttaaaagtc
    44041 catattcagt cacatttctc aacgaccaga taaaagtact taaaagccgc aagattgaaa
    44101 atatcagtat aaaggacact ttaaccgtca ggctcaaggc cgggctgagt acctgcctgc
    44161 aactcgacgg ggtcaagtcg gcctttgaat tcatatcaga cgcaaacatg catgaataca
    44221 actacaggat aactgatatt gtttccttcg atgatgaagc tgcctatgaa attgaatttg
    44281 aaccaagggg ggaagttgat ttaccaaaat tcaaaggctc gatttttatt aatacttcag
    44341 attacgcaat actccgtgca gacttcgaat taagctccag gttccttgat gagatgaaag
    44401 aagcatttat ttcaactgcg tcaaaaggat tcagaacatg gccgatatcg gtcagatatt
    44461 ctgttagtta tcgcaatgtc aacggcagat attttctgaa tcatgtgaga ggggacctgc
    44521 tattcctgtc aagggagaaa aaaaggcttt tcaattcacc ttttgaagta ttttttgaaa
    44581 tggcaataac aagcatgaag accgataacg ttgtccgctt tgagagagag gaacttgctc
    44641 caatacattc ggttttttcc aggacaattt caaattacga ctcagagttc tggggggatc
    44701 aggatttcct caaacctgaa gagaatcttc ttcacgcact tcggaacatg aaagtcaggc
    44761 tgcagcaatt cccggaatga tcatatttcc ggctccgtaa cggatgcggc tgattccgaa
    44821 ccgacatccg gtgataaacg gaaaggatca atatttgttt ataagcttat agagttcggt
    44881 aagatcgggg gtgagtataa tttccgtcct tctgtttttt tgacgcgctt cgggagtatt
    44941 gttcaggtcc agtggtgcat attcgccttt gcctgcagca gtaagccgtt caggtcttat
    45001 cctcgaacct tcaagcaaaa ccctgacaac cgttgtggcc cttttgacac tcagatccca
    45061 gttgtcctta agctggctgt tgcccgggtt atagggaaca ttgtcagtat gaccctcaat
    45121 ggtaatctga atccccggat tccgctcaag cacttcggcc agattcctaa gggctttcct
    45181 gccgtttgca tcaatatccc agctcgccga cctgaataaa agcttttcat cgagcgatac
    45241 ataaaccttg ccatctcttg cagctatcga aagtccgttg ttctcaaaac ccagaagtgc
    45301 ttcggagacc ttgcccttaa ggtcctgcac aagctttttc tgtgcatcga gtatcttttc
    45361 aagttcggcc atcctggcat ttcttttttc aagctcgtat gtaagctcgt caagcgagat
    45421 cttcttcagg tccagcgatg tttccagttc gcgcaatata ttttcctttt gcctgagatt
    45481 ttcctgtgtg ctaatcagtt ctgccagaag ctttttggtt tcattcacgt taccccttat
    45541 aaggtcctgg tgggcattct gcatgtcatt cagtcgcgat tccacaatac tgagatcttc
    45601 ccgggccctg tttctttccg actcagcagt ggagagcctg tatttcatct cctttatttc
    45661 attctcaagt gccgaggcat tgttatcaag ttcccggttc agcatctgga gtcttatatt
    45721 ctcagctttg aattcatcac ggtccatcat gttctgccgg cttgtaatct gaagctcacg
    45781 gtattttttt ggagaaacac aggatatggc ggagagcagc aatataatac acgccagcga
    45841 ggggattgtg gttttcttca ttttttagta taggttaatg attccggatc agactctttt
    45901 cccggttctg ctccatgcag cccggccatg tcacaaaaat aagaaaaacc ccattaaggc
    45961 ttgcctaaac acaagggaat aataattgct atatttgcac catagcaaat ctttttaaga
    46021 caggatgaac ggtggggaac atctgctgtg gagaatttca actcctgatg acctcaggaa
    46081 gcttaaacca gctgatcttg taaaagtatc tgctgaactc cggcaatata tcatcgatgt
    46141 ggtaagcaat aatcctggtc atctgggagc aagccttgga gtggttgaac taaccgtggc
    46201 tcttcactat gttttcaata caccgtatga caagataata tgggatgtcg gacatcaggc
    46261 ctacggtcac aagatactta ccggaaggaa ggagaaattt ttaaccaaca ggaaatataa
    46321 gggtataagc ggattcccta aaatgtcgga gagtgagtat gatgctttcg ggactggtca
    46381 ttcttcaact tccatatcag ctgccctcgg tatggcggtc gcatcaaaga ggaaaggaga
    46441 aaaaagaaat gtcgttgccg ttattggcga tggagccatg acagcaggac tggcattcga
    46501 aggtcttaac aacgccggca ttgcaaaatc ggatataatt gtgatactca acgataataa
    46561 tatcgcaatc gatcctaatg tcggtgcgtt gaaagagtat ctcctcgaca ttgcaaccag
    46621 taaggcgtat aacaggttcc gggacaggat ttggaacatg ctgggaaaat tcggaagaat
    46681 aggtccgaat gcccgggtac tggccaagca ggtacaggcc ggatttaaaa gtaccatcct
    46741 cgacagaagc aatctgtttg agggaatgaa tttcaggtat ttcggtccca tcgacggcca
    46801 tgacatcctt catctcatac agatccttga ggacctgaaa aggatacccg ggcccaagtt
    46861 gctgcactgc ctgacggtaa agggaaaagg gttcaaaaag gcggaggaga atcagacagc
    46921 atatcatgca ccggggcgat tcgacaaaaa taccggcatc attatcaggt cttcagcaaa
    46981 agattctttg gtcacatacc aggaaatctt cggaaggacc attcttgaac tggcaaaaaa
    47041 taatgacaaa atagccggga tcacacctgc catgacaacc gggagctcac tctgcattat
    47101 gatgaaggaa attcccgaca gggtatatga tgtcggaatc gccgaacagc atgccgttac
    47161 tttttccgcc ggactggcca cgcagggaat gatcccttac tgcaacatat actcttcttt
    47221 catccagagg gcctacgacc aggtaattca tgtcgttgca cttcagaaac taaaggttat
    47281 attttgtatt gacaggggag ggcttgtagg cagtgacggg gcaacgcacc atggattttt
    47341 cgatcttgcc ttcatgcgca gcatccctaa catcatcgtc agtgctccga tggatggacc
    47401 ggaactcagg aacctgcttt atacagccca gcttgagaaa ataaccggcc ctttttcaat
    47461 acgctacccc aggggtaact gtatgatttc tgactggcag aaacccttct tcgaaattga
    47521 gaccggcact gcaaggctga tcagcagtgg ccgtgatatt gctgttttaa gtatcgggca
    47581 tcccgggaat tttgttgttg aggcaggcag gatactggcc gaagaaggta tctccgtcac
    47641 tcattatgat atgaggttcc tcgctcctct ggataccagg gctcttcata cggttttccg
    47701 ggaattcgga aaaatcatca ccgtggaaga cggtatactg aaaggtggat ttggcagtgc
    47761 tgtcattgaa tttatgtctg acaacggcta taaagcagag gtaaaacgcc tgggtattcc
    47821 cgattatttc gttgaacagg gtacgcagca ggaacttatc agggaatgcg gctttgaccc
    47881 ggaaagtatc gcacagacaa taagggaaat ggtgatgaaa agagtaccgg agcataaact
    47941 taaagcctga acagtttacg ggctttttac ctgccacaaa atctcttttt ataacacatt
    48001 attggatact gaaaaaacag gttttatcaa aaactgaatt atcatgagaa atcttttagt
    48061 gattacggtt atcctgctgc tgatttcctg cggtaccaga caacaacccg ctgatcagga
    48121 atggatacag cttttcaacg gaaaagatct taacgactgg agcgtgaaga ttaagggtta
    48181 caagctgaac gacaattcca ggaatatttt cagggtggaa gacggggtca tgaaagtcag
    48241 gtacgatgac tttgataaat tccgggatga attcggacat attttttaca aaaaaccctt
    48301 ttcaaggtac aaactccgga ttgaataccg gttcaccggc gatcaggtac ccggggcacc
    48361 agcatgggcg tacaggaaca gcggcgttat gctgcattcg cagtcaccgg aatcgatggg
    48421 agtggatcag gactttccgg tatcagttga aggtcagttt ttaggtggtg acgggataaa
    48481 cgaacgttca accggaaacg tctgtacacc aggtaccaat atcgtaatgg atggcgagct
    48541 aatcacacag cattgcatca actccagctc ccggacctac catggtgatg tgtgggtcag
    48601 ggctgaattc gtcgttctgg gcgacagtgt tattcatcac attattgaag gtgacaccgt
    48661 cctgacatat ttcaagcctc aggtcggagg tgctcctgag gattatcccg tccccgaagg
    48721 gacactgctg aaccagggct atatctgcct gcaggcagag agtcacccgg tagagttcag
    48781 gaaggttgag ctgctggatt tgtcaaaagg cagaaagcaa aagtaaaaag ataaaagttt
    48841 ttattatttt cgctatcctg ccagtaacgg atcatattgg tttatgcccg gatggcaggg
    48901 ccgagcgaca ggcaaacacc cggtgggtgt ttgcagcgag gaaccgggta tgagcggtga
    48961 ccaattccgc cacccccctc agaaggcaga gttcttttta tagatttgta atccaatatt
    49021 atctaccagg tttttattat gcccggatgg cggaattggt agacgcgctg gtctcaaaca
    49081 ccagtggcag caatgctgtg ccggttcgat cccggctccg ggtacaaaaa cagagtgagt
    49141 gcgagggcga gagcgccctc ttctcttttt tactcaccct gatacacact ctctgttatg
    49201 cccggatggc agggggttgc gttaaaaaaa cacccggtgg gtgtttttag caagcagcca
    49261 gcctgcaggg ccgcgtagac gcgctggtct caaacaccgg gccgagcggc aagcaaacac
    49321 ccggtgggtg tttgaagcga ggagccagtt tgcaggggtg gatgaatgct gtgccggttc
    49381 gatcccggct ccgggtacaa aaacagagtg agtgcgaggg cgagagcgcc ctcttctctt
    49441 ttttactcac cctgatacac actctctgtt atgcccggat ggcagggggt tgcgttaaaa
    49501 aaacacccgg tgggtgtttt tagcaagcag ccagcctgca gggccgcgta gacgcgctgg
    49561 tctcaaacac cgggccgagc ggcaagcaaa cacccggtgg gtgtttgaag cgaggagcca
    49621 gtttgcaggg gtggatgaat gctgtgccgg ttcgctatcc ggtgacgggc agacgtcatg
    49681 acgaattaga actggattca gctgccaggc agaaactcaa tagagtttct caccaagtgc
    49741 agccttaagc ctgtaaaccc cggctgcata atcaattctt gatttgagaa ggatgagcct
    49801 gctttctgat actgcagtat ttgcatcaag cacttcaagg tttgtgattg tacctgcccg
    49861 gaagcttatc ccagcaagtt cataggcttt cttcgcctgt tcaaactgaa gggcagactg
    49921 agaaatcttc tgtgccgctg actccaggta cgattgtgcc tcctgtattt ctgaaataat
    49981 gctccgtctg atgttttctg tttcatatcc ggcattattg attaccgatt gagcttgtct
    50041 cagtttatat cttgtcttca ttccgtcaaa aattggtatt gaaataccaa atgcagccga
    50101 ataattggcc ctcagggtat tgaggtcagg ctgatatccg ttttttactc cacccgccat
    50161 ggtcatactt atgagaggtt tgtttgcagt tcttaacagc tggtattgca gttctgcaag
    50221 agaagcattt tccttactga tgagtatctc gccgcggtga agcaatgcat attgctgtaa
    50281 cgatccggta tttattaagg gaagttcaat gccaagatcc ttcatgaccg gccttttaaa
    50341 tttttctatc ccgattagtg cacccatata cgcctgctgg acagtcagtg aatacagaag
    50401 atcagttttc tgactctggg ctgcagaaat cctgacctcg gttgaaagaa tctggtaatc
    50461 cgttgcggag cctgtggcct gcattgccct gatatacctg agatgttctt caagggtcgc
    50521 cagttgttca ttttttattt ttactgcttc ctgcagatat aaaatcgagt agtaaccatt
    50581 tatggcggca agtgacattt tctgttttac cagttccagg gtctgggtgc ttatggtttt
    50641 attccctgtt tcaagcttaa cattctgcct ggtcctgccg aaatcatata ttatctgcct
    50701 gtaatttaca gcacccgaat aattattttc aggaaataat tgcatttccc caatctgagg
    50761 tatgtcaatt ttcaataccg gtccaatgtt ggaaaatcct gcagaagcgt caataaccgg
    50821 gtaataacct gttcttgcca ggctgattct ggcatctgca tttttcagag tctcttcagc
    50881 ctcttttact gaaggatgat tttttataat ctcttctatt aatatttcca gtgacaatga
    50941 atcattgctg acaacctggt tttcagggtt atcagcttta atgcctgccg gtaaaagcca
    51001 gatggttaaa caaacgattg ccggtatttt tatcatatta ttcctgggta aagtttttgt
    51061 gagcattgct tttttttgtg cgtatgaaaa atataggtat tccgccaatc agcgtaaata
    51121 ttcctgaaag cagaaaatca tcattaattc cttctatata tgcctgttta tttagattgg
    51181 acaagatgag agaagatgca gcagattcaa ctttcacagg agggattccc gcattatgct
    51241 ggagatgaaa tcttaacctg tctgttgtaa cattatatgc ctgggatcgt gcattcagag
    51301 ctgttccaaa ctgctgattg tgatatattg tcctgactgt aagcatagtg gcgagaagtg
    51361 ccaccccaag gctaccgccg agctggcgga ttgaattgga gatacctgat gcctgtgcca
    51421 tctttgcccg cggtatctca agaagagcca cggtattaag agcagtgaaa gtcaggccca
    51481 ttccaaatcc tcttaaatac aatgagacca ttatggatct tatttcgctc agataggaaa
    51541 gtttcgaatt caggtaaaaa ctgaaagaca tcagaagtat tccgataatt attggaattt
    51601 tcggatttat ccggtctgag attctcccgg agattggtgc aacaattccc tgaattatac
    51661 ctaccggtat aaaaaccgct ccggcttcca gcgcagtata acccagggag ttctgcagat
    51721 agaccggaaa aaggaaggta ctcccgaaca ttccgatgct aaaaaataca agaataataa
    51781 gtgctaatcc gaaattatgg tttaacacca gtcttagatc gatgatcgga tcttccgccg
    51841 tcagttctgc agttataaaa acagtaagtg caatcagggc gatggcagcg caaagcagta
    51901 tgtaaggagc atgccaaccg gcagtatttg tagccgcagt ggtctctgac aatgcaaata
    51961 atgtgagagg tagaaatatg gttattgaaa taaatccgat aaggtcaaat ttcctctttg
    52021 caggatgttt gtattcagac tggatgacaa ttgtaaaaag tatggcgatt attcctacgg
    52081 ggatattaat accgaatatc agcggccatg aaaaattgtc gattatgaag cctcccaggg
    52141 ttgggccaaa tgatattgat gcagcggcag caatacccca gaatccaagt gccagacccc
    52201 gttgttttac aggaaactcc ctggttatta tagccatacc gagcggctga agcgtaccgg
    52261 cgcccagacc ctgaataatc ctggaaacga tcagtatatc ttcatttggt gaaatgctgc
    52321 aaagaaatga tccgatggta aagcccgaca gtccgagaga atacatcctt ttgtatccaa
    52381 acctgtcggc cagccatccg gaagtgggaa gcatcaccgc catcgccagc atatatgcgg
    52441 ttattatcca ctgtactttg tcgagcccgg tcccgaatga agccattatt ttcggaagtc
    52501 ctgtattgac gacagtaccg tctaggacag ccatgaatgt tcccaacatg acatttccaa
    52561 ggataaacca tttgtatttt aaatgtttcg gatgaataac tgaatccggc tttcggaaaa
    52621 aggcacgtat cctatgtagc agaggctgtc gctttatcat tctattttct tgatattttg
    52681 accattgcag acattcctga aagtatgccg tctgatggca attcattgcc gttactaaca
    52741 gaatcaattg agatcctgat tggtattctt tgcgttactt ttgtgaaatt acctgaggca
    52801 ttgtttgctg ggatcaggga aaacacagag gcagtacttg caccaatgcg aaagaccttc
    52861 ccatggaact tcctgtgagg aaaagcgtca attgtaaacc tgacgctctg gccgggatgg
    52921 atttctgcaa ttttcgtttc ttcaaggtaa gctgatatcc atttattacc gtcatcagta
    52981 acggtaaaca ccggctgtcc aggctgaatt atatctccgg ggagaagcca tctttttgaa
    53041 atgacaccgt ctgacggtga gtataatctg gtatttccaa gttgggtctc aattacctta
    53101 acctgggcgt cggctgtaac tgcagctgca taagcagatt tgacctgtgc ctccgaaaca
    53161 gacaaaagag ccctggcagc ttcaacctgt gcagctgcca cttcgctcgc tttcagggca
    53221 tgatcatatt gctcccgggt gatcacgccc ccttcgagct gttgttcagc cctttcaaaa
    53281 tcttcccagg ccttttcata gctgatcttc acaactttca gtccctgtcg gtctgaatga
    53341 agcttaaaac ccgactgcga cacgctgaca agagcctgat tcttcactgc cagtgcctgc
    53401 accttctgtg ccgacagatc agtactgtca agtactgcca gaagctgtcc ctttttgaca
    53461 cggtctccct catcggcaaa cagggatact atcctgccgg gaattttgga actcactgct
    53521 gaattatcag catcaacatg tgcatcatca gttgaaatga atgaagaata atctctgtac
    53581 caaaaccagg cgcctgttaa cacaataaca attaccagtc ccaatggaat ataaatcttt
    53641 atattatgtt tattgctttt ctgtctcata tgagtgaaaa ttactgaggt tgttaaaatt
    53701 atttgtataa aagtccttta ataaaaacat cggacatgtc tttcaccttt tcagaaagaa
    53761 gagtaaattc tgcatcatct gccatcagca gatcctttcc tgaaaggatc agcttgatct
    53821 gtcctctaat aatatcaaga aaaagagttg caattttata agtgtcatct atcctgaaaa
    53881 gatcctgcct gatgcctttt tccaatatca ataccagtgt cctcttttcc tcctcccgga
    53941 atatttccag cagattggca ataacaggtt tcagatgatc ataggaatcc agcgtgagtc
    54001 tgcccagatt actaagcttt ctgaaatagg aaatccttga ttgtgaaaat ttcctgagat
    54061 tatccaccgg atcattggaa ttttctattt cagtgtgaag aattctcagg aattcatcat
    54121 gctcccttgt aagaacactc ttataaaggc tttctttatc agggaagtag taatatagag
    54181 ctgcttttga aattctgaga tccctggcta tttcccgcat cggggttttt tcaatgccaa
    54241 aaagcgcaaa tcttttctgt gcagcagcta ttattgcttc agccttattt tctttctgtt
    54301 tatcatcact tttcatttct ccggaaaaga gtcgcaaaaa taacaaataa cctgaccagt
    54361 tcgttcagat ggtcagatga tttttttatt aatatcgtga ttgaggacct actttcgaaa
    54421 aaacctttgt gccggtttcg tcttttcaga cgctgataca gctcaatatt gtgttaacta
    54481 gaatagaaaa tatccctgct atggttgagc cggaagtcgg cagataaaat ctaacccccg
    54541 actttggggc acgggaagcc gttagaaaaa cttggcaact atgaatacag aaccagggat
    54601 ttttgtacgg acgtacgggt ctgcgtcacg tttttccaca gggtgacagc cggaaaaacc
    54661 gatcaggacg cactgaatcc tgtcatggtt gtaatcagtc ttcagattcc ggggaaaaat
    54721 atccccggca gaggtgaaag caggccggag cccctgaaaa ggaagcgccg ttaagttaca
    54781 acttgagatt tattcacgtt cctaaaaagc atatttatgc gcaggcattg tcgtaaagaa
    54841 gaaaatgggt ttgtcaaccc tgagtatctt cagcggacaa tgcttaacac ccttgggaac
    54901 aaaaataaga cagcttttgg taatcacatg acgttctcca tctatccaga gctcaatctc
    54961 accattaagc tcattgggat tgtcgggatc gctgccataa agtccaaaaa cctcatcggc
    55021 atcatgggta tgctccgtta cccacacaat atcaggatag gctttgtaaa gccatgagca
    55081 ttctgcatag taggcacccg gcacaacctg tgaatcaaga taggtaatct gtttcagcat
    55141 atcagcaggc tgctggccga gattggtggc ttccggcggt gtctccggcg ggggcgcagg
    55201 ggtatagaca atgtttttcc cgtaatcagg tttatctacg gttcccttgt caccggtttg
    55261 cccgttaaga tcatttagca ggaacagggt aaacagtacc gacaaaatgc atgcgcttaa
    55321 agttactctt ttcatgtgaa aatagattta tatactactg aagattaagt ggtccacatg
    55381 gaatccacat gaataatatt ttcatccttg tttgtgattt tgtatcatg
//
LOCUS       DAOOYG010000005        48763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_597_length_48763_cov_7.607067, whole genome shotgun sequence.
ACCESSION   DAOOYG010000005 DAOOYG010000000
VERSION     DAOOYG010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 48763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_597_length_48763_cov_7.607067"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PK288_01025"
     CDS             complement(<1..1216)
                     /locus_tag="PK288_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPM17526.1"
                     /translation="MISSIFWIVPVAALTALLFAWIFYRSMKVQEEGTERMKEIAAYV
                     REGAMAYLRRQYSVVTKVFIILVILLAVLAYFGIQNPFVPIAFLTGGFFSGLCGYLGM
                     KTATFASNRTAWAASKSLNKGLKVALRSGAVMGLVVVGFGLLDIALWFLILNYLVFTP
                     EHMETGFKFLGLTLVHPGTTEHIKLIEITAVMLTFGMGASTQALFARVGGGIFTKAAD
                     VGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYAGSILATAALGA
                     ALPLASGMTQIQAVIAPMIVSAIGIFLSIAGVYFVRTKESATPQALLRALLLGTGGSS
                     ALILVVLAIMAATGMITWGVFGSTVAGLLAGVIIGQSTEYFTSDANKPTKGIAKQAQQ
                     GPATVIIEGMAVG"
     gene            complement(1434..2198)
                     /locus_tag="PK288_01030"
     CDS             complement(1434..2198)
                     /locus_tag="PK288_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17527.1"
                     /translation="MSKYVYLTCTPEALVASMLPPEGFGMYLSTGTKKRNKGQTIFLE
                     VDLGQIEDLIDMESLERRCVEKEDGSPKSSVYLSVYRALEIIPLSALKSLYLTTDHGT
                     ALELKKTVYDKTREDPNTLRLYQELCPVTPLVASGLPPSAFLKVLTDGSRPISLPKLL
                     FVELKLGELATNPLYGSAEQLPYSNIGHLRDCLEILRGEYEKHMKTVQRIYSGVLLYR
                     TIETGFYVGSKDEIYFYPYPSMKELEDINYEFFREI"
     gene            2571..3560
                     /locus_tag="PK288_01035"
     CDS             2571..3560
                     /locus_tag="PK288_01035"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HPM17528.1"
                     /translation="MEKIRIGITGYGNLGKGVESGLEHFPDMELKAVFTRRDPAGISI
                     RSHGVPVLRYDEIGSYRDRIDVMILCGGSATDLAEQAPVVAGMFNTVDSFDIHARIPE
                     YYGRVNEAALSSRHACIISVGWDPGLFSVARVYSNCILPNGNDYTFWGPGVSQGHSDA
                     VRRIKGVKDAKQYTVPVESAIEKVRRGENPELTKRQKHTRLCYVVPEEGADLNSIERM
                     IRTMPDYFDEYATTVSFISAETLHREHGRMPHGGFVIRSGATGENGINRHIIEYSLKL
                     DSNPEFTSSVLLAYARAAYRLAMRSDFGAKTVLDIPPAMLSALSGAELREKFL"
     gene            3616..4200
                     /locus_tag="PK288_01040"
     CDS             3616..4200
                     /locus_tag="PK288_01040"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPM17529.1"
                     /translation="MTSRPTAYGYDLKSSLKILSEGGIILFPTDTIWGLGCDATNPDA
                     IKRIFRTKKREDSKSLIILVNGFGMLERHVTFVPEVAINILEVADKPLTIIYPQGKNL
                     APGICAADGSAGIRICLDPFCNELITRFRKPIVSTSANISGSASPSIFREIADEIKAS
                     ADYIVSHRQQETRKNYPSSVLKINSDGTIKIIRP"
     gene            complement(4203..4904)
                     /locus_tag="PK288_01045"
     CDS             complement(4203..4904)
                     /locus_tag="PK288_01045"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPM17530.1"
                     /translation="MKTQLFSVSLLAASLFICEDLCGQTRENRDVSGFSNISFEIPGN
                     LVIRTGPQFRLVLEGPGSNLKDIETKVSGGRLVIRKVNRWSSFRNDNVTVSLTLPQVE
                     GLSVSGSGRAEISGPIKTHQLSLNVSGSGKVITGDLETDRLECSISGSGDIILRSGGS
                     AEKGEITISGSGSFSGENVRVANLDVGVSGSGSCKCHATRTLNAKVSGSGRVIYAGDP
                     EIDARVSGSGRVMSK"
     gene            complement(5005..5529)
                     /gene="rimM"
                     /locus_tag="PK288_01050"
     CDS             complement(5005..5529)
                     /gene="rimM"
                     /locus_tag="PK288_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPM17531.1"
                     /translation="MGSAPYKDKALLGRISKIHGYDGTVIVKTEPGFTENISETEPVF
                     LEVDGIPVPFFIADLGYPGGDILKVRFQDYDSIERISEFLGCRVYVTSAGSGGSNVQN
                     NDLTGFRVFAGKGNYLGTVDTIIHKPGQDLLSVRPEGKKAILIPLHRDLILEIDEKSG
                     IIVMDLPEGLTGLG"
     gene            complement(5556..6143)
                     /locus_tag="PK288_01055"
     CDS             complement(5556..6143)
                     /locus_tag="PK288_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPM17532.1"
                     /translation="MAVKIRLARRGRRKKVFYHIVVADSRSPRDGRYIEKIGIYNPLT
                     NPATIELDFDRALGWLQNGAIPTETCRAILSYKGVLLKKHLLEGVKKGAFDDAEATRR
                     FEEWMKQNLAKIEAKKSGLEKSREDELSKRIAAEKKLNEARAAQLAKKQAEMAERQRA
                     ESQAEQAEDQAEKQAAEAAEETPSAEVDSAAEAEA"
     gene            complement(6274..7620)
                     /locus_tag="PK288_01060"
     CDS             complement(6274..7620)
                     /locus_tag="PK288_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM17533.1"
                     /translation="MKLRHIIFSAIMLLIFPVSCDKMLDLAPHQNVSGTISLGSDQGV
                     RKVLIGAYARFAGPSIYGGCILRNSELLGAGDECRWTGVFTGPRQIFMKQIDPANEDI
                     TSQWLDSYEVVSIANTVLSAIDMVNETDRDRVEGEALFLRSLIFFDLVRFYAQQYRYG
                     ELNSQYGIPLVLTPASRSEKPAGKGRDSVEDVYDRVIADLQEAALKLPNENGAYASSG
                     AATALLARVYLQKGDYLNAGNAANEVILSGKYELLSDFKDAFNNDENSPEDIFATQIT
                     PQDQRSSMTEFFSVPEYGGRDGDIEILERHLSLYSPGDRRLSLFFQGNGAMRTGKWNN
                     PYGVVSLIRLAEMYLIRAECNFRLGTNLGDTPLNDYNVIHTRAGLSPAHSVTLDDILL
                     ERRLELAFEGFRIHDQRRLHQEVGTLPFDAPALVFPVPAREISADPVLAGQQNEGY"
     gene            complement(7642..10716)
                     /locus_tag="PK288_01065"
     CDS             complement(7642..10716)
                     /locus_tag="PK288_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM17534.1"
                     /translation="MNKLYLLIFISCFYPGLLSAHEKIITGAAGTAENEPAVRAVLLS
                     DPGNMMFSETDWMNSIDAGEASGPEFSAEDMKTTGVITGSDDADNAKPVSDQPGQNDT
                     VMTGYGIREKSLVTGSIVRVDGASISSAPVPTVEMALQGRVAGVFIESLSGKVAGSAR
                     MRIRGATSVTAGNDPLFVVDGIPLATESLNQSGAAINPLLPFNINDIESVEILKDGSA
                     AAIYGSRGANGVVLITTKRGISGDTKLNFTLQRGFSQPSARRNFLNSEQYIRYFEEAA
                     YNSDRYEGLDPVNNPADYSGSWLEFARSRFERYSGWAAYNDPNLAVNTDWQDQAFRTG
                     SLLTADFSARGGNDNLRFFASGSYTSREGILISNGWERITGRLNLDNKVNRFLDIGVS
                     LSLSGTNVDQIPADNSFSSPMQLVALAPITPVRDKKGKLYNTPITTYYNSLIDTEDAS
                     RKISNLRTLANSYLNFRILENLVWKNEIGIDLLNLKENCSYGKRTESGEDTGGYGFAN
                     YGEIRNISPRSYIDYSGRSDNFGISGVLGTEYQYTVIDNAWLTGQDFPTDDLRTLSNA
                     GLISGSSSERAEYSYLSWFSRINFDYRYKYIISLSARVDGSSRFGKKSRYGTFPAASA
                     GWVLSREGFLADAAALSLLKLRVSTGLTGNTGIGNYSYIGLFGTDNYNGSSGLIPGQV
                     PNENLKWESTLQTNVGLDFGLFENRISGGIDYYIKNTSDLILNVPVPGTSGNRVQAQN
                     LGSMRNEGFEFVLNTTNTAGALLWSTNVNFSVNRNKITDLGENSLIDEGSSVFMNVAM
                     VGQPLGVFYGAEYAGADPANGDALWYVNPRDAQGNITDHTTTTSDYSKAGFVVLGNPN
                     PPVLFAITNSLSYRNFVLSFMFQGVSGNKIHLAGDSFLASNATWFDNQTTDQLNSWKK
                     PGDQTDIPQARLGYVNGNQSRNSRYVSPGDYIKLRTAMLSYDLPGSFLNRLGLSGARV
                     YVQGHNLLTFSKYRGWDPEVSADFMVNSLRSGSDFYSAPQPRTVLAGISISL"
     gene            11261..14746
                     /gene="dnaE"
                     /locus_tag="PK288_01070"
     CDS             11261..14746
                     /gene="dnaE"
                     /locus_tag="PK288_01070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPM17535.1"
                     /translation="MTAFSHLHVHTQYSILDGASHIPNLIERVKSLGMEAVAITDHGN
                     MFGVKEFHSAATRKGIKPIIGCEVYVAKRSIGETAGKEDRSGDHLILLAKNLTGYKNL
                     VKLVSTSWIKGFYYTPRIDKDLLKKHSEGLIASSACIAGEIQDEILNGSMERAEAVLR
                     DFLDIFGDDFYLEIQRHETNDPEADRTVFPKQQQVIEAFRKLSVKYNVKIIATNDVHF
                     VNAEDAEAHDRLICINTAKDFDDPDRLRYSKQEYLKSEEEMRRIFSDIPEAIDNVGEL
                     VAGIEKYKLDHDPIMPDFQIPDGFSDKNGYLRHLTYAGAAERWGNPTREQTERIDFEL
                     ETIAKMGYPGYFLIVQDLLRAAREMGVSVGPGRGSAAGSAVAYCLRITDIDPIKYGLL
                     FERFLNLDRISMPDIDIDFDEDGREDVLRYVVNKYGHDKVAHIITFGTMAAKMAIRDV
                     ARVQRLPLAEADRLAKLVPERPGVTLSAAYTEVPELAKEKDSPNKLVSQTLKYAEVLE
                     GSIRQTGVHACGIIIGRDALDNYIPLCTAKDTDLYATQYDGSHVESVGLLKMDFLGLK
                     TLSIIKDAVENIKRSRGTVINIEKLPLDDKKTFELFSNGETTGIFQFESTGMKRYLRE
                     LRPNRFEDLIAMNALYRPGPMEYIPKFIRIKHGTEPINFTLPVMERYLNETYGITVYQ
                     EQVMLLSQELAGFTKGEADTLRKAMGKKKKSIMDEMKLKFFEGCIRRGYDETIVNKIW
                     SDWEAFAQYAFNKSHSTCYALIAFQTGYLKANYPAEYMAAVLSRNISDIKKITIFMDE
                     CRRMGMEVLGPDVNESNVKFTVNRDGNIRFGLGAIKGVGENAVLQLIEEREKNGVYKS
                     IYDLAERVNLNSLNKKNLEAMAVAGAFDCFTDISRSQYFAIDTRGVSFIENLIRYGNN
                     AKSIKNSSQQTLFGSTGGFDIIKPDAPSCPDWPKLEKLNKEKEVIGIYLSSHPLDDFK
                     LEINNFTNATLADLQNLSEWYERDVIVAGMVTDSKNGIGKNGKPYGSFTLQDYSDSFR
                     FVMFDKEYIEFSKFFITGYYLLVKGRVQKHKFRKDELEFSIKSINLLSSVKDELVKSV
                     TVKINMEIVNMAMISDLLELMKENRGESELKFLFYDQEDKISLSMFSRTIRIRLNNEL
                     ISFLDDYPGIEYKVN"
     gene            14790..15110
                     /gene="trxA"
                     /locus_tag="PK288_01075"
     CDS             14790..15110
                     /gene="trxA"
                     /locus_tag="PK288_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPM17536.1"
                     /translation="MAQDVNDSNFDEIVINSDKVVLVDFWAEWCGPCRMITPIVEDIS
                     KEYADRVVVAKCDVDESPGVAARFGIRNIPTVLYFKNGAVADKQVGAVPKANFVKKLD
                     ILLQ"
     gene            15313..15888
                     /locus_tag="PK288_01080"
     CDS             15313..15888
                     /locus_tag="PK288_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPM17537.1"
                     /translation="MTVCVFAASSSRIDKKYAEAARELGTLIASSRIQVIYGGGGIGL
                     MGVLADAVISGGGIITGIMPSFMNDEGWGHPRVMDMILTSDMAERKKNMFSRADAVVA
                     LPGGVGTLEELTEAITLKQLGLFRGPVVILNTLGFYKSLIGFFEHMISNHFLRPEHRG
                     IWTVADTPGEVIRALRNNTRWIDDPRRIARI"
     gene            complement(16012..17067)
                     /gene="ribD"
                     /locus_tag="PK288_01085"
     CDS             complement(16012..17067)
                     /gene="ribD"
                     /locus_tag="PK288_01085"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPM17538.1"
                     /translation="MPENNDMKFMRRCLDLASKAEGMTYPNPMVGAVIVHQGNIIGEG
                     YHIKAGGPHAESRAVESVRNKKLLEESTLYVNLEPCSHFGKTPPCADMIVSWKIPKVV
                     VGTIDTSAKVSGKGISRLRDAGCEVVTGVCEDECRRINRRFLTFHEKKRPYITLKWAE
                     SSDGFIDRERDEDYRIEPNWITGRAERILVHKWRASEQAILVGAGTVRADDPRLNVRE
                     WKGSNPIRIILSGSGSLPDDPAVNRSIGSLIVFTFYPEKVVLPGSVIMKLNHREPAAK
                     QISEALHGWGIQSLFIEGGAQVLSHFIKTGYWDEACVFTGTKAFKAGVKAPSVEGKTI
                     MEKTFSNSTLKRLLNNK"
     gene            17094..17957
                     /gene="prmC"
                     /locus_tag="PK288_01090"
     CDS             17094..17957
                     /gene="prmC"
                     /locus_tag="PK288_01090"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPM17539.1"
                     /translation="MAFNLQTIRDIRNHISGELKEIYPEEEIQSLFEIMVLHILGGSK
                     AGLLGNPQRRLTNEESERIMLFCNELKTGRPVQYITGETVFYNCRIRVGPEVLIPRPE
                     TEELVDLVLRENRNFSGTITDACTGSGCIAIALARNLPAAGITGLDNTPAALKTASGN
                     ALLNRVSINFILSDILKNGIADAPKPDIIVSNPPYVRESEKALMHRNVLEFEPHSALF
                     VPDDDPLVFYRAILESGNRVMDRGGKIYFEINEAMGESMVNLMESLNYIGIRVVSDIN
                     GRDRIIKGTLK"
     gene            17954..18421
                     /locus_tag="PK288_01095"
     CDS             17954..18421
                     /locus_tag="PK288_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPM17540.1"
                     /translation="MNKKNGLKTALNRAMALCASREYCSADIRSKLESWGICTTEAEK
                     IISGLIREKFLDDSRYAHAFVRDKFKYNKWGRMKIAAGLRQKKIAEEIIRNALGEIDE
                     ARYMDMIRETLNLHRKSVRAKNQYELRGKLLRYGLSKGFENHFLYDILNDLDP"
     gene            complement(18678..19112)
                     /locus_tag="PK288_01100"
     CDS             complement(18678..19112)
                     /locus_tag="PK288_01100"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HPM17541.1"
                     /translation="MAISRYVPADRDSYLVINLVLATVILLVFIYSGIFSPGKDNYPV
                     VCIHEKVTGQPCISCGLSHSLSLMIRGRIAEARTWNANGPAIFIFFAAQLIMRILFSA
                     CRVKYPSTGKELIIYDISGSTVLFLVAFFPFLINIFSLSGIH"
     gene            complement(19118..19660)
                     /locus_tag="PK288_01105"
     CDS             complement(19118..19660)
                     /locus_tag="PK288_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17542.1"
                     /translation="MEDQINNTGQGSAGSSPGAGYTGSRTGQNLGIAALITAIVTFVF
                     AVIPCVGLIAIIPGVIAIVLAVVGLSRASESESRGVLIAGLVIAVVASIISFSQLFVA
                     GKIIEKAKRGFGGDIENIIRDVQKDVLQDLEDAKISIKVESNGDKVEIKADTGSRSRI
                     EELEELERTDSLNSGTNVKK"
     gene            complement(19682..20263)
                     /locus_tag="PK288_01110"
     CDS             complement(19682..20263)
                     /locus_tag="PK288_01110"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPM17543.1"
                     /translation="MKILPWISAKGLVLFILLLISCGCGTGKKSKGNNGDPGVATQQG
                     TSSTNWAEQSVWLLGYFSPSLLLRPPYSEWYDSEYEGYQPRHDVVEKLSSLGNNDLTI
                     VIVLGIWCPDSRREVPRFMKIIDSIGFPVRQLTFIGVDNEKLSPLGDYGKLGIERVPT
                     FIVYEKKVELGRIIENPVTSLEQDLLNILTGKQ"
     gene            complement(20309..22459)
                     /gene="scpA"
                     /locus_tag="PK288_01115"
     CDS             complement(20309..22459)
                     /gene="scpA"
                     /locus_tag="PK288_01115"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPM17544.1"
                     /translation="MRPEFYFDDLHNCFSSGSADERVMSERKAEPIITAEQIPLKAFY
                     SKADLSGIEHMDYAAGIPPFLRGPYASMYIVRPWTIRQYAGFSTAEDSNAFYRRNLAA
                     GQKGLSVAFDLPTHRGYDSDHERVAGDVGSAGVAVDSVLDMKILFDRIPLDRMSVSMT
                     MNGAVLPIMAFYIVTALEQGARLDELSGTIQNDILKEFMVRNTYIYPPEFSMRIVADI
                     LRYTSEKMPKFNSISISGYHMQEAGASCDLELAYTLANGFEYLRTGIKAGLNIDDFAP
                     RISFFWATGMNHFMEIAKLRAARLLWAKIVNTFGPLNPKSLALRTHCQTSGWSLTAQD
                     PFNNVGRTCIEAMAAVLGNTQSLHTNAFDEALALPNDFSAAIARNTQLYLQRETDICR
                     AIDPWAGSYYVESLTDALARSAWKHIGEIESMGGMTKAIETGLPKMRIEEAATRVQAR
                     IDSGVQTIVGINKYKPDNEEPVEILEVDNSMVRRAQIKRLEMLKAGRNEDECMRSLDA
                     ITECVRSGKGNLLELAVEAAAKRASLGEITFACEKITGRYKAVIRTISGVYSSEYKSD
                     EEFTKARVLVEEFAAKEGRQPRILIAKTGQDGHDRGARVVSTGYADIGFDVDIGPLFQ
                     TPAEAARQAVENDVHVLGVSSLAAGHRTLVPQIILELKKLGREDILVVAGGIIPPGDY
                     QFLYDAGVAAIFGPGTPVSKAARQILEILLHNSD"
     gene            complement(22456..23826)
                     /locus_tag="PK288_01120"
     CDS             complement(22456..23826)
                     /locus_tag="PK288_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPM17545.1"
                     /translation="MKQKKLFEEFPATSTGEWLDRIRAELKGADLNEKLLWKTGEGFD
                     LMPFYRREDMEKLRYTDAFMPLYLGKDGLKCRNSKEDPGQLGKSKGDKAASWLVRQDV
                     LVQSYASANEQALSLLERGVDSLGFLIGDPDTISNENFAMLLRDFNPSCAEINFSSEG
                     KAIEIVSLVSDLLADKEPGRKSVRGAVEADPLVRLMIQGKLCIPVDAGLDYLAALVRK
                     AMDLPYYRSIHIHGTYFRKAGSGPVLELALAIAMAVEYIDQLTSRGIDPGTAASAIRF
                     SFGIGPSYFMEIGRLRAARLLWEVVCRKFGVTDDFPARMEIHSETVLNAAAGDPFLNM
                     IGSQAAAMSAIIGGTDSLTVHPVEYRNGMTGDFPERIARNQQLLLKEEAFFDRVADPS
                     AGSYYIETITSHIAENAWNIFLQIEKKGGFLQALNSGMIHTLLHDRKDHGEKAGDRKG
                     ENYKLS"
     gene            complement(23950..25039)
                     /gene="prfB"
                     /locus_tag="PK288_01125"
     CDS             complement(join(23950..24975,24977..25039))
                     /gene="prfB"
                     /locus_tag="PK288_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_144267993.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPM17546.1"
                     /translation="MLTNEQIKDLSERCEALRRYLDVDGKLLQIAEKEALTRAPGFWN
                     DPRAAEDLLREISRIKSWTTQFEKVAKSLDDLQVMYDFYKEGEASEEDADNQYEQSLG
                     LVEDLEVRNMLRKDEDQLGAILKINSGAGGTESNDWASMLMRMYLRWAERNGYKVREL
                     DFQSGDEVGVKSVTLEIDGERAYGYLKSENGVHRLVRISPFNAQGKRQTTFASVFVSP
                     LVDNNIEIEINPADITWETFRSSGRGGQNVNKVETAVRLRHHPTGIVIENQETRSQLN
                     NKENAMRILRSQLYELELRKLREEQARIEGSKKKIEWGSQIRSYTIHPYKMVKDHRTN
                     KESGDVMAVLDGDLNSFIRSYLMEFGGN"
     gene            complement(25169..27424)
                     /locus_tag="PK288_01130"
     CDS             complement(25169..27424)
                     /locus_tag="PK288_01130"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPM17547.1"
                     /translation="MSDTDTIKEKKETVQKIVKKQYPVTGMSCASCSANVETMLGKQP
                     GVLNVFVNLASEAATVEFDSSMVRPEDLKAVVTSIGYDLITDESETAKEELESLQLKR
                     YRTMRRRTIWAVILAVPLVLISMVFMNIPHAPYIMWALATPIVFLFGRSFFINAGKQI
                     IHGATSMDTLVSLSTGIAYLFSVFNTLFPGFWESRGLQAHIYFEASGVVIALVLLGKF
                     LEERAKANTSSAIKKLMGLQPKTVFKVLPSGEIREFLISKVKPGDIILVRPGEKIPVD
                     GTVTSGGSYVDESMITGEPVAVYKEEGLKVFAGTINQKGTFRFTAEKVGADTLLSSII
                     KYVKEAQGSKAPVQKLADRIAGIFVPVVTVLAVITLAVWWIAGGENGFVQGLLSMITV
                     LVIACPCALGLATPTAIMVGIGKGAENNILIRDAEALELACKMDTLILDKTGTLTEGR
                     PTVENIEWETPGDKEELAAILAGLERLSEHPLAEAIVRHFDNNMPETRFENVKSITGK
                     GIEGLHNGRKYLAGGQGFISENKIPVAPRLSGLAETWEENARTVVMFADETEVLAVLS
                     INDSLKETSADAVGQIKKLGIDVRMVTGDNRKTAAAVAARAGITEFAASMLPAAKAEY
                     VEALQKENRIVGMVGDGINDSQALARADVSIAMGKGSDIAIDAARMTIISNDLAVIPA
                     AIRLSRNTVRTIRQNLFWAFIYNIIGIPIAAGVLYPLNGFLLNPMIAGAAMALSSISV
                     VSNSLRLKYTG"
     gene            complement(27689..28513)
                     /locus_tag="PK288_01135"
     CDS             complement(27689..28513)
                     /locus_tag="PK288_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HPM17548.1"
                     /translation="MLEIPESKTISLQAGKILSGRRITDVFSPTSHHKFAWFSGDPAE
                     YARLLSGKTVKSTFGHGMFVDILCDGESCITIGEGTIMRYHAPSEEPPGKYQLLITFD
                     DNSHVAFNVAMYGGIWAYRGIFENRYHQLSLNSISPLDDAFDEDYFDVILNDSPENIS
                     VKALLATEQRFPGLGNGVLQDILFNAGIHPKRKKSSLSGRDKADLFISVRDTLKIMTE
                     KGGRDTEKDFFGNEGGYKTILSRNTFQHPCRVCGDQIRKEAYLGGAVYFCLTCQRL"
     gene            complement(28769..29656)
                     /locus_tag="PK288_01140"
     CDS             complement(28769..29656)
                     /locus_tag="PK288_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HPM17549.1"
                     /translation="MDNKHQKEKVIPGIDFHLLNLILKGLGQIMLQENALTGLLFLAG
                     IFYGSALMGLAALVAVTSGTLTAVLLKYDTGNLNKGLYGFSASLTGVAMMLFFKPAPV
                     TWMAVVAGSVLATVMQHFFLRMNIPVFTLPFVLVTWLALVLFGRFAPDLLATPGALPV
                     NVANPLSYSIRGFGQVIFQENVISSVLFFLAVFISSPVAALYGIAGALVSGVLATLSG
                     APAGPVGSGLYSFNAVLCAMVFAGKKLKDAIGVFISVLLSLLISFIMHHFNITQLTFP
                     FVAASFLVLSFSKHVNKKP"
     gene            complement(29856..30452)
                     /locus_tag="PK288_01145"
     CDS             complement(29856..30452)
                     /locus_tag="PK288_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17550.1"
                     /translation="MNMKTPPSFSGMRILPVVFLIVLFCSCSKEKEENDQLDKDYKGT
                     LRFEYTRSFPTFSAIIDMDVDLYRSGDIIISQPSSEHFDATDEIPNTVKLRETGDIII
                     TSLSGSYKTIDGSPYVIINANTLIDGTMTVWRWDDDRGWIFPIDIPYTNENPVESPMN
                     FDFHKVTMSEDIIGGTVPAFQGNLTVRWILGLMPALPK"
     gene            complement(30577..31998)
                     /locus_tag="PK288_01150"
     CDS             complement(30577..31998)
                     /locus_tag="PK288_01150"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPM17551.1"
                     /translation="MTIADLYSMMPLIVIAMAPVVMMIVISILRNYEVIYGFSVLAFI
                     VAFASLFFTGSFLPRSIEPLLIVDVYSLFFLGIIIASALLVTILSHDYIGTLGGVKEE
                     YFLTLFTSTLGASLLAVAGHFVIFFLGLELLSVSLYILVAFQRSKDTSVEAGIKYLLL
                     ASVSSAFLLFGMALIYTGTGTMQFAGVASLLKGSDAIAGFVSVGFSMMMVGIGFKLAL
                     VPFHMWTPDVYQGAPAPVSAYIATVSKGAVMAVLIRFFFDIRGYDSRFFFVVISALAI
                     LSMFIGNLLALKQQNIKRLLAYSSIANMGYLLVLLLTGTDKGIQAAVFYLISYFVTTI
                     GAFGVITLLSISREETEKIDDYRGLFWTRPWAALVLTLSMLSLAGIPFTAGFIAKFYL
                     ILEGMKAGLMLLIIVMIINSVIGLYYYLRVITSMFTPGDDAGLPAISLNGNMALVLIT
                     LGILILGIYPGWLINIITRLVSL"
     gene            complement(32002..33618)
                     /locus_tag="PK288_01155"
     CDS             complement(32002..33618)
                     /locus_tag="PK288_01155"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPM17552.1"
                     /translation="MVRDVLYNRYSIDPDIHDIEMILAYLILILLAGSLLALLTGRKG
                     NLWPRVISVAAVSLDLVLLIIFSSSVSGTDGKWLMDLQLEWIPRFGISLHLALDGLSL
                     VMVLLTLFLGIISVVISWKEINSRVGFFHFNLLLILAGIIGVFLSLDLFLFYFFWEMM
                     LVPMYFMIGIWGHENRTAASYKFFLYTQASGLLMFIAILALVFVHESTSGTLTFDYVQ
                     LLGLEMPAAKELLIMSGFLAAFLVKLPVVPLHNWLPDAHSQAPTAGSVILAALLLKTG
                     AYGLIRFVVPLFPSASAAFAPVGMGLGVIGILYGAKLAFAQTDLKRLVAYTSVSHMGF
                     VILGVFAFNVIAYQGVVMQMIAHAISTGALFILAGQLYERIHTRDMDQMGGLWEKTPV
                     MGAIGMIFSLASLGLPGLANFVAELLTLLGTFKASVLMASLACLGLVAATIYSLRIVQ
                     KVFFGKMNHEWEIADLNLREKIVSAVLVVLVVALGLFPRPVLNLAGPAIIKTLDSHPA
                     QLTGLPDSGKSAGNGKTASFNQLSGMFKKH"
     gene            complement(33555..35435)
                     /gene="nuoL"
                     /locus_tag="PK288_01160"
     CDS             complement(33555..35435)
                     /gene="nuoL"
                     /locus_tag="PK288_01160"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPM17553.1"
                     /translation="MMETCIALIPLIPLLGFLILAVAGGKFDRKTAGYIGAGSVGISL
                     VLALIAGALFLRSLPGVSSYSVSLWEWISAGELRADMSFSLDSLSLVFCIVITFVGFL
                     IHVYSIEFMSGDEGFSRFFAYMNLFVGSMLILVLADNILLLYFGWEGVGLCSYLLIGF
                     WYKEPENGYAARKAFIVTRIGDTAMIIGIFILFVNFRTFHLDELMLQVNDKWSVGSPL
                     AVITAALLLFGALGKSAQLPLQTWLPDAMAGPSPVSALIHAATMVTAGVYLIARCHVL
                     FNLAPVVMTAIAIIGALTLLIAGFSALGQHDLKRILAYSTISQIGYMFLALGVGAWSA
                     AIFHFMIHAFFKALLFLAGGVVILVLKEEHDIFRMGGMKKEMPLIFFTFLAGSASLSA
                     LPLVTAGFYSKDPILWYSFSSVTGNFWLWIAAFTGAFLTTLYTFRLVFVVFFGETKLK
                     PSFQPGKLMTVPLAILAFFSIVAGFIELPESFGRFHLFSSLAENTLPAVTVRETGYPE
                     TLFQLLSALIVFAGIYLAYLAYYKKPSMSESFEHGRIHRFFESGFGFDRLYDVLLVNP
                     LVWLSAINRNDIFDWLNIGLARLTVLGNRLFSLTQNGKLRWYVMSFTIGIVLILTYMI
                     LR"
     gene            complement(35440..35757)
                     /gene="nuoK"
                     /locus_tag="PK288_01165"
     CDS             complement(35440..35757)
                     /gene="nuoK"
                     /locus_tag="PK288_01165"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPM17554.1"
                     /translation="MLTVPIETMLIFAGLLFLIGLVGLLTRRNLIFMLMSIEIMFNSA
                     GLVFIIAGRHWKQADGQVMFIFILAVAAVEVAIGLALLLQVYHHYKTLDADSVGKLKD
                     NNQ"
     gene            complement(35761..36276)
                     /gene="nuoJ"
                     /locus_tag="PK288_01170"
     CDS             complement(35761..36276)
                     /gene="nuoJ"
                     /locus_tag="PK288_01170"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPM17555.1"
                     /translation="MNFFFYLAAAIALISTIMAISGRNAIHALLNLIISLLAVSVIFY
                     LLDAPFIAALEVIIYAGAIMVLFIFVTMMLNIGTEKKIEKRWLNPRVWIVPSILAALL
                     LVDFILIINGLQIPEPVDETLNTKQVGISLFSTYVLAVEIAGILLMAGVIGAYHLGSQ
                     KKKVTHRFLKK"
     gene            complement(36289..36804)
                     /gene="nuoI"
                     /locus_tag="PK288_01175"
     CDS             complement(36289..36804)
                     /gene="nuoI"
                     /locus_tag="PK288_01175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017402052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HPM17556.1"
                     /translation="MISILRSIWLTFLHIFHKRETVEYPEVKPYLAPRYRGRIVLTRD
                     PDRNERCVACYLCAAVCPVDCISLQAATGEKGRRHPEFFRINFSRCIFCGFCEEACPT
                     NAIQLIPDFEMGEYIRQNLVYEKEHLLISGEGKYHGYNFYKHAGVAMGVKGKGEGDTE
                     YKPVDVKDLMP"
     gene            complement(36829..37770)
                     /gene="nuoH"
                     /locus_tag="PK288_01180"
     CDS             complement(36829..37770)
                     /gene="nuoH"
                     /locus_tag="PK288_01180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPM17557.1"
                     /translation="MNYLWIILGVIFIALSIAAGLIWVERRLLSVWQDRLGPNRVGPF
                     GLAQVIADTLKLFFKEDWVPPFSDKVVFTIAPGILMVTILLTFAVIPFGPEFCVFDSN
                     IGVLFFLGNSSLGVYSIVLGGWASNNKYSLIGAMRASAQMLTYEVFMGLSLMGVVMMA
                     DTFSFSEIVEAQRGGWFILPQFLGFVVFFIAGIAETHRLPFDIPEAEGELIAGYHSEY
                     SGMKFGMFFVGEYLGITLISAIIATMFFGGWLGPSFLPPVFWFLLKTFIFIGVFILLR
                     ASLPRPRYDQLMKLGWKLLFPIALLNLLVTGAILIFL"
     gene            complement(37781..40504)
                     /gene="nuoG"
                     /locus_tag="PK288_01185"
     CDS             complement(37781..40504)
                     /gene="nuoG"
                     /locus_tag="PK288_01185"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HPM17558.1"
                     /translation="MEMKVVNIRIDGKPYEAHEGKSLLDTALSLGLYIPHFCYHPALG
                     SVGACRLCAVKKFRDTNDTRGRIVMSCMEPVVDGLIVSINDPDAKAFRAAVIESLMTN
                     HPHDCPVCDEGGECHLQDMTVMSGHNYRRFDFRKRTHNDQYLGPFISHEMNRCIQCYR
                     CVRFYRDYAGGRDFNVFATHNNVFFGRHEDGTLQNEFSGNLVEVCPTGVFTDKTFREH
                     YTRKWDLSNAPSVCVHCSVGCNIIAGERYDTLRRIMNRYNGAVNGYFICDRGRFGYEF
                     VNGKDRILSPRTRDSRDLEQAEMPADQVFTALEPLFSKDRKIMGIGSPRASLESNYAL
                     LRLVGEENFFHGISGKEQSLTRAALQFMQNSGIVVPSLKQVEKADAVIILGEDLTNTA
                     PMMALAVRQAARNIQITEALAKGIPFWNDLPVRELAQDRKSPVYIATPVKDSLDDVAE
                     RSFRGTPAGISKLGFNISSVIKRQAIVPGDNDDEIRNLAEQIARTLLKAENPLVIAGI
                     TCLDGNVLNAGMSIASSLLSLNKKVMFIPVLPESNSMGLALMPGKSLDDAADTLSVQG
                     TDTLIILENDLFRRADEDTVANMFGKSGRVVVLDYLNNPTARAADILLPATAFAESQG
                     TIVNNEGRAQRYYKVLAAGEGIKESWQWLNQVSGIRSDNVPVSPGHFDDIVASMVTAL
                     PVFSAIKEYIPDADFRMLNAKVPRQTFRYSGRTAMNAHRAVSEKRVRQDPDSPLAFSM
                     EGRRESPPSSLVPFYWTPGWNSVQAFYNYIDHPNGSMKGGDPGIRLIRQAERVNSGSF
                     EMITGYFESEGDGWLIVPLYRVFGSEELSSAASSLRQLVDDPAILVNRRDAERLNVDD
                     GDSVRLETSGKRLVVKVKIENSLVSGVAGLTVNLPGMNFVDLPASGEILKI"
     gene            complement(40495..41769)
                     /gene="nuoF"
                     /locus_tag="PK288_01190"
     CDS             complement(40495..41769)
                     /gene="nuoF"
                     /locus_tag="PK288_01190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPM17559.1"
                     /translation="MERPLTGHIKKGEAPPGFREYYRAGGYNGTIKALKEMTPAGVTS
                     LVKASNLLGRGGAGFPTGVKWSLVPMEGENIFPKYLVCNADEMEPGTFKDRLLLEGNP
                     HQLIAGMIAAAYAIQAGTAYIFLRWAYKKAERELRKAISEAYREGFLGKNILGTGFNL
                     DIHVHTSAGRYICGEETALLNALEGKRGIPRTKPPFPATSGLFGKPTVVNNVETLSWI
                     SHIVEKGADWFKGLSLTGTGGTKLYGVSGRVKRPGLWELPLGVTIGEIINEHAGGMHD
                     GYAFRGVLPGGASTDFLVEEHLDVKMDFSTVAAAGSRLGTGTMIVLDDRTCPVAFLQN
                     VEKFYALESCGWCTPCREGLPWVEKMLSAIEDGKGKMEYLDILKFHTSYMGPGHTFCA
                     HAPGAAEPLQSGLRFFHDDFKRHIEEKKCPWK"
     gene            complement(41775..42239)
                     /gene="nuoE"
                     /locus_tag="PK288_01195"
     CDS             complement(41775..42239)
                     /gene="nuoE"
                     /locus_tag="PK288_01195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPM17560.1"
                     /translation="MLTEEEKKEILEEAELYPYPAAACIDALKIVQKHRGWVSDESVG
                     DIAEILRMSEDEVDGVATFYSRIYRRPVGRNVILICDSISCMIMGYKTLYKYISEKLE
                     ISFGETTSDGRFTLLPVSCLGDCDNAPAMMINDDHYNNLTVEKIDELLEKYK"
     gene            complement(42257..44011)
                     /gene="nuoC"
                     /locus_tag="PK288_01200"
     CDS             complement(42257..44011)
                     /gene="nuoC"
                     /locus_tag="PK288_01200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF008728.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HPM17561.1"
                     /translation="MSSDNSGISDVLIERFGSRAGFVRQETKDGITTLWTEREFIVPV
                     LKYLKTEVPGPFKMLYDLTAIDERARESNPVQPGGDFTVVYHLTSLDRNEDIRLKVPL
                     KENDLAIPTLTGVWESANWYEREVFDMFGIRFDGHPRLARILMPESWKGHPLRKDHHA
                     RATEQDPFVLTEQKRDLEDENLRFKPEDHGLARSAEDSDFMFLNIGPQHTGTHGLLRL
                     VVQLNGEDIVNIVPEIGFHHRGAEKMGERQSWHTFIPYTDRIDYLGGVMNNLAYLLSV
                     EKLAGIEVPDRAMVIRVMMSELFRIASHLVWLGTFAQDLGQLSPVFYTFNDRERIFDI
                     VIAITGARMHPSWFRIGGVAHDLPDGWQKLMQDFVNYLPARLREFDKVIIKNRIFKAR
                     TVGIGVYTAEEAIEWGLTGSGLRAAGYDWDYRKKRPYSGIDRFDFDIPLGKNGDCYDR
                     AVVRMEEMRQSLRIIQQCIDNMPAGHYKSDHPLVIPPRKEKTMTDIETLITHFVGVSW
                     GPVIPAGEAMVPIEAAKGSNGYYLVSDGSTSSYRSRIRTPSFPHMQMLPFISRGYTIP
                     DLLSILGAMDFVLADLDR"
     gene            complement(44036..44659)
                     /locus_tag="PK288_01205"
     CDS             complement(44036..44659)
                     /locus_tag="PK288_01205"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPM17562.1"
                     /translation="MKKTGAINTGFEKELSGRSLYDSFVFARLDELLAWARKNSIWPF
                     GFGLSCCFVEMATSLTSRYDLARFGGEVIRGTPREADLMVVAGTVFMKMAPIIKRLHE
                     QMMEPRWVISMGSCANSGGMYDIYSVVQGVDKILPVDVYVPGCPPRPDALLEGIVLLR
                     DSIGREERPLSWVIGPNGVYKAEKPCMRDLKKDERSRMTNIRPMNEV"
     gene            complement(44656..45072)
                     /locus_tag="PK288_01210"
     CDS             complement(44656..45072)
                     /locus_tag="PK288_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPM17563.1"
                     /translation="MEQQITFWPFIVFAAIVVALAAVMIGLSHVLGERHSEKKTGEPY
                     ESGIPPTGDARLRFSSAFYIIAMFFVIFDLDAAFIMLWAISFRELGLAGYIGVFIFIV
                     LLIILLVYELRIGALDFGPDGKKILKNIPRPQKDTK"
     gene            complement(45349..45474)
                     /locus_tag="PK288_01215"
     CDS             complement(45349..45474)
                     /locus_tag="PK288_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17564.1"
                     /translation="MKEAPEGANCFLSGGEMIDYVPPAERPEYAFAAVNNHYNPR"
     gene            45653..46252
                     /locus_tag="PK288_01220"
     CDS             45653..46252
                     /locus_tag="PK288_01220"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPM17565.1"
                     /translation="MLKPFLYHNLIEAGCDEAGRGCLAGPVFAAAVILPRNFRHDLLN
                     DSKKLTSLQRFRLRDEIIRSAVAWKVASVSNEEIDEMNILRASIKAMHLAVGGLVRKP
                     QFLLIDGNRFFPYSGIEHRTIIRGDAMFLSIAAASVLAKTFRDEFMEKIHNEFPDYGW
                     DRNKGYPTRQHREAILRLGITRYHRRSFNLLNTQMKLDF"
     gene            46324..48477
                     /locus_tag="PK288_01225"
     CDS             46324..48477
                     /locus_tag="PK288_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPM17566.1"
                     /translation="MKKLLSLMAILVIVAGIRARADEGMWLLPLIEKLNMGKMTELGL
                     KLSAEDIYSLNKPSIKDAIVIFGGGCTGEIVSSRGLLLTNHHCGYSQIQAHSSLENDY
                     LRDGFWAMNMEEELPNPNLSVTFLISIEDVTDKVLADVRPEMNERERNSKISIARNRI
                     ESKATECNDYMATVSSFYGGNYFYLLVYQRYTDVRLVGAPPSSIGKFGFDTDNWEWPR
                     HTGDFSVFRVYTGPDGKPAPYSKDNIPLRPSYWLPVSIKDLNAGDFAMILGYPGRTQR
                     YFTSFEVDELMKITNPNRIRIRGIRQEILMADMQSDPKINIQYASKYSGSSNYWKFSI
                     GQQAGLERLKVMEKKERIEKQFNDWIAADGERKLKYGESLNMIRSAIEERAEYLNAQQ
                     YLNECFRQGCELLNLSTLASMLTPSLKSGDKQKTEGYIARIKSYAESFYRNYNPPTDE
                     KATKAMIELYRQDVPVKFHPDFFGSLIDKKYKGNIDRFTADVFSKSIFANESRFNAFL
                     ANPVLKTLENDPVYIMSNSVSDMSTSVSRNLSRYDEDLATGRRLWIAALREMIPEKTL
                     YPDANSTMRLTYGTISDYDPKDAVTYKFYSTLQGVVEKYKPGDYEFDVPQRLLDLNAR
                     KEFGRYASPKGYMPVCFLTTNDITGGNSGSPVMNARGELIGLAFDGNWEAMSGDVAYE
                     PDLQRTIAVDIRYVLWVMDIYAGAGHLVDEMTLVQ"
BASE COUNT        13119 a        12361 c        11067 g        12216 t
ORIGIN      
        1 taccaacagc cataccttcg ataataactg ttgccggccc ttgctgtgcc tgctttgcaa
       61 tacctttggt gggcttattg gcatcggaag taaagtattc tgtcgactgt ccgatgatca
      121 caccggcaag aagaccggct acggttgatc cgaatacacc ccatgttatc attcctgttg
      181 ctgccattat tgcaagcacc acaaggatga gtgcggaact gcccccggtt ccaaggagca
      241 gtgcccgaag caatgcctgg ggagttgccg attccttggt cctgacaaaa tatacaccgg
      301 ctatcgacag gaaaataccg atagcagaca caatcatcgg agctataaca gcctgtatct
      361 gggtcattcc cgatgccagg ggaagtgcgg ctcccagtgc ggcagtagca agaatcgaac
      421 cggcatatga ttcatagagg tcagcgccca taccggctac gtcgcccaca ttatcgccca
      481 cattgtcggc aattgttgca gggttcctgg gatcatcctc aggaatacct gcttccacct
      541 ttcctaccag gtcggcacct acgtcggcag cctttgtaaa gataccaccg ccaacacggg
      601 caaacagtgc ctgtgtggaa gcacccatgc cgaaggtaag catgacggct gtgatttcta
      661 taagcttgat atgttcagta gtaccaggat gaacaagggt gagtccaagg aacttaaaac
      721 ctgtttccat atgctcggga gtgaaaacca ggtaattaag aataaggaac cacagggcta
      781 tgtcgagaag gccaaaacca acaacaacca gccccatgac tgccccgctc ctcagggcca
      841 ccttgaggcc tttgttaagg gatttggaag cagcccacgc tgttctgttc gatgcaaatg
      901 tggctgtttt cattcccagg taaccgcaaa gacccgaaaa gaatcccccc gtcagaaaag
      961 caatcggtac aaacggattc tgaattccaa agtaggcgag aacggcaagg agaataacaa
     1021 gaataataaa taccttggta acaaccgaat actgtcgcct caagtaagcc attgcacctt
     1081 ccctcacata ggcggctatc tccttcatcc tttccgttcc ttcctcctga actttcatgc
     1141 tccggtaaaa gatccaggca aaaagaagtg cggtaagggc agccacagga acaatccaaa
     1201 aaatactact aatcatgata tctgttttta aatttgaaaa attataggtt tggatgtgaa
     1261 aaattttgca ggtacaaaac taattcagat tttcatttat gcaaatgaaa aaaaagatat
     1321 tcttcaaact gtttgaaagg ggaagaatgc actcagccgc ctcaggtacc gtactgctgt
     1381 ttaatcatat ttgcacaggc atgaaaccac ctgcgtgaaa accaccggta tcctcagatc
     1441 tcccggaaaa attcgtagtt gatatcctca agctccttca tggaaggata tggatagaaa
     1501 tatatttcat cctttgatcc cacatagaat cccgtttcaa tggtcctgta aagcagtacc
     1561 ccggaataaa tcctctgtac ggttttcatg tgcttttcat attctcccct caggatctcg
     1621 agacaatccc ggagatgtcc gatatttgaa tatggcagct gctcggcaga gccgtaaagt
     1681 ggatttgttg ccagttcgcc aagcttgagt tcgacaaaaa gcaacttcgg aagactaatc
     1741 ggtctcgatc cgtcagtgag gactttcagg aaagccgagg gtggaagtcc gctggcaaca
     1801 agcggtgtca cgggacataa ttcctggtaa aggcggaggg tgttgggatc ttccctggtt
     1861 ttgtcgtata ctgtcttctt caattcaagg gcagttccat gatcggtagt caaatacaga
     1921 cttttcagag cagaaagcgg tatgatctca agagccctgt aaactgaaag ataaacagaa
     1981 cttttaggac tgccgtcttc cttctcaacg catcttctct caagactctc catatcgata
     2041 aggtcttcaa tctgaccaag atccacctcc aaaaaaattg tctgtccctt attacgtttt
     2101 ttggtaccgg tcgaaagata cattccaaat ccttcaggag ggagcatcga agccaccagg
     2161 gcttcggggg tacatgtcag gtaaacatat ttgctcatgg taatatattt ttaggaacat
     2221 tcatcagaac aaatcacttc atggaaccag aatctttgcc tatatcaaat ttaggcttct
     2281 tttttcaact ttaaaagtaa ataataacat ccggctgttt attttccctg gtttctgatg
     2341 attttttaaa aggataaaac tgttagttcc caaacaccgt taactcaatc ataaaattcg
     2401 gggaaaaagt tcctggtgcc ggttaaattg aaaatcatac cggagttgcg tttttttcag
     2461 tcgctttact cgccacccga tacaagaata tgaggttttc agttacattt gaggcaaatt
     2521 ctgcagccga aaacagacaa gcagagaata aaaaacaaaa tcccagcaca atggaaaaaa
     2581 taaggatcgg aataacaggg tacgggaacc ttggcaaagg tgttgaatca gggttggagc
     2641 acttccccga tatggaactt aaagctgttt tcacaagacg tgatcccgca gggatcagca
     2701 ttcgttcgca tggcgttccg gtactccggt atgatgaaat cggcagctac cgggacagga
     2761 ttgatgttat gatactctgc ggcgggagtg ccaccgacct cgcagagcag gctccggttg
     2821 tggcagggat gttcaacacc gtagacagct ttgatataca tgccaggata ccggaatact
     2881 acggcagggt aaatgaggcg gcactctcat cgcggcatgc atgcataata tcagttgggt
     2941 gggatcccgg attgttttca gtggcaaggg tgtactcgaa ctgtatcctt cccaatggca
     3001 atgattatac tttctggggc cccggggtga gccagggtca ttccgatgcg gtgagaagaa
     3061 ttaaaggcgt aaaggatgcg aaacaatata cggttcctgt cgaaagtgca attgaaaagg
     3121 taagaagggg agaaaaccct gagcttacaa aaagacagaa gcatacacga ttatgctatg
     3181 ttgtgccgga agaaggtgcc gaccttaatt caatcgaaag gatgataagg accatgcccg
     3241 actatttcga tgaatatgct acaaccgtca gttttatttc agccgaaacc cttcacaggg
     3301 aacatggcag aatgccgcat ggcggcttcg ttatcaggag cggagccacc ggggaaaacg
     3361 gaattaacag gcatatcata gaatactcac tgaaactcga ttcaaatccc gagttcacat
     3421 cttcagtcct gcttgcttat gcgagggctg cttacagact ggcaatgaga tccgattttg
     3481 gtgcaaaaac cgtacttgac atccctcctg caatgctttc agcactctca ggtgcggaat
     3541 tacgtgaaaa attcctctga ccaggtactt aaagcctgaa aaccgtttga ttcagatcat
     3601 ttacaatcag ataaaatgac atcccggccc actgcatacg gctacgacct caagtcatcg
     3661 cttaaaatac tgagtgaagg cggcattatt ttgttcccga ccgataccat ctggggactg
     3721 ggatgcgatg caacaaatcc cgacgcaata aaaaggattt tccggaccaa gaaaagggaa
     3781 gacagcaaaa gccttataat tctcgtaaac ggattcggga tgcttgagag gcacgtaact
     3841 ttcgttcctg aagtcgcgat caatatcctg gaagtggcag acaaaccgct taccatcata
     3901 tatccgcagg gaaagaacct ggcacccggg atatgcgccg cggatggttc ggcaggcatc
     3961 aggatatgcc tggatccctt ctgcaatgaa ctgataacca gattcagaaa gccgattgta
     4021 tcaacctcgg ccaatatcag cggatcggct tctccttcca tattcaggga aatagctgac
     4081 gagataaagg cttcggctga ctatattgtc agccacaggc agcaggaaac cagaaagaat
     4141 tatccttcgt cggtccttaa gattaacagc gacggaacaa taaaaattat ccgcccgtag
     4201 gtttatttcg acattacacg gcctgatcct gaaacccttg catcaatttc cggatcgccc
     4261 gcataaatca cccgcccgct tcccgatacc tttgcattca gtgtccttgt agcatggcac
     4321 ttgcaactgc cgcttcccga tacaccaaca tcaaggttgg caaccctgac attttccccc
     4381 gaaaagcttc ccgacccgga aatggtgatc tctcccttct cagctgatcc tccggatctc
     4441 agaattatat ctcccgaccc tgagatggag cattccagtc tgtcggtttc caggtcaccc
     4501 gttattacct tcccgcttcc gctgacattc aggctgagct gatgagtttt aatcgggccc
     4561 gaaatctcag cccttcccga tccagaaacg cttaaccctt caacctgcgg caacgtgaga
     4621 ctcacagtca cattatcatt cctgaacgat gaccacctgt ttacttttct tattacaagc
     4681 ctgcctcccg aaacttttgt ttcaatgtct ttcagattgc tcccggggcc ctcaaggaca
     4741 agcctgaact gcgggccggt cctgatgaca agattgccgg gaatttcaaa gctaatattg
     4801 ctgaaaccgc ttacatcgcg gttttccctg gtctgaccgc ataaatcttc acaaataaac
     4861 agtgaagcag ccaggaggga aactgaaaat aactgggttt tcatttttaa gccggatttg
     4921 atttctactg tttattccaa aagacgcaaa aaaaatcaaa cagctgcatg tttgaacttt
     4981 ttccctataa acagtggtga cgactcagcc gagtccggta agtccttccg gcagatccat
     5041 tacaatgatc cctgattttt catctatttc caggataaga tccctgtgca acgggatcaa
     5101 tattgccttc ttaccttccg gtctgaccga cagcagatcc tgcccgggct tatggattat
     5161 ggtatcaacc gtacccaggt aatttccttt gccggcaaat accctgaaac ctgttaaatc
     5221 attattctgc acattactgc ctccactgcc ggctgaagta acgtagaccc tgcagcccag
     5281 aaattcggat attctttcaa ttgaatcgta gtcctgaaat ctgaccttca gtatatcgcc
     5341 tccagggtat ccgagatcgg caataaagaa aggaaccggt atcccgtcaa cttcgaggaa
     5401 aaccggttcc gtttcagaaa tattttcagt aaaacccggc tcagtcttaa caatcacggt
     5461 accgtcatat ccgtgaatct ttgatattct gccaagtaat gctttgtctt tatatggtgc
     5521 agaacccatg acagtccagg catttgccgg aattatcagg cttctgcttc ggcagcggaa
     5581 tccacttctg cagacggagt ttcctcagca gcttctgcag cctgcttttc agcctggtct
     5641 tcagcctgct cagcctggga ctctgccctc tgacgttcag ccatctccgc ctgttttttg
     5701 gccagctgtg cggcccttgc ttcattcaat ttcttctccg cagcaattcg cttgcttaac
     5761 tcatcttccc gtgatttttc cagtccggat tttttagctt cgatttttgc caggttctgc
     5821 ttcatccatt cttcaaacct gcgggtagcc tctgcatcgt caaaggcgcc ttttttcacg
     5881 ccttcaagaa gatgcttctt aagaagaaca cccttatagg aaaggatagc cctgcatgtc
     5941 tcagtaggta tggcaccatt ttgcagccaa ccaagggccc tgtcgaaatc aagttcgatt
     6001 gtggccggat tggtaagcgg attgtaaatc ccgatttttt caatatatct gccatcccgt
     6061 ggcgacctgc tatctgcgac cacaatgtgg taaaagacct tcttcctacg gcctcttcta
     6121 gctaatctga ttttaactgc catctaagta ttacacgttt gtttaaccta ctaaaaaaat
     6181 ttgtgggtgc aaagataaat aatttaatta accaaacaat gaatactgaa gatgactgcc
     6241 gggaaatatt caggctgtta tgccttaagg cgcctaatac ccttcgttct gctgcccggc
     6301 aagaaccgga tctgccgaga tctcccttgc cggtaccggg aataccagtg cgggagcatc
     6361 aaatggcagt gttccgactt cctgatgcag ccttcgctgg tcgtggatcc tgaaaccttc
     6421 gaatgccagc tcaagtcttc tttccagaag aatatcgtca agtgtgactg aatgagccgg
     6481 agacagtccc gcccgcgtat gaatgacatt ataatcattc aggggagtgt cgccaaggtt
     6541 tgttcccaat ctgaaattgc actcggccct gatcaggtac atctctgcca gtcgtatcag
     6601 actgacaacc ccataaggat tgttccattt ccccgtcctc attgcgccgt tgccctgaaa
     6661 aaacaggctc agcctcctgt cccccggcga ataaagcgac agatgtcttt cgagaatttc
     6721 aatatccccg tccctgccgc cgtattcggg aactgaaaag aactctgtca ttgaactccg
     6781 ctgatcctgc ggggttatct gtgttgcaaa aatatcctca ggtgagtttt catcattgtt
     6841 gaatgcatcc ttaaaatcgg aaagaagttc atacttcccc gaaaggatca cctcattggc
     6901 cgcgttcccg gcattcaaat aatcgccttt ctggagataa acccgggcca gcagtgcagt
     6961 ggcagccccg ctgctagcat atgccccgtt ctcattggga agctttaagg cagcttcctg
     7021 aagatctgca ataacccgat cataaacatc ttccaccgaa tcccttccct tccctgccgg
     7081 cttttcgctc ctgctggcag gagtaagcac aagagggatc ccatattgtg aatttaactc
     7141 accgtacctg tattgctgtg cataaaaccg cacaagatcg aaaaaaatca ggctgcgcag
     7201 aaacaatgct tcaccctcaa ccctgtcgcg gtctgtttcg ttgaccatat caatagcact
     7261 gagcacagta tttgcaatgc tgacaacttc ataactgtca agccattgac tggtaatatc
     7321 ctcattggca ggatctatct gcttcatgaa aatttgccgc gggcctgtaa aaactcctgt
     7381 ccaccggcat tcgtcacctg ccccgagaag ttccgaattt ctgagaatac agcctccgta
     7441 aatggaagga ccggcgaaac gtgcataagc tccgataagg accttccgta caccctgatc
     7501 tgatcccagg gatatcgttc cgctgacgtt ctgatgaggg gccagatcga gcatcttatc
     7561 gcacgatacc gggaaaatca acagcataat tgctgaaaaa ataatatgcc tcagtttcat
     7621 gactatcagg gtttcatgag tttacagact gatgctgata ccggcaagta ccgtcctggg
     7681 ttggggagcg gaataaaaat cagatcccga tctcaacgaa ttaaccataa aatccgcaga
     7741 cacttccgga tcccagcccc tgtatttgga aaaagtcagg agattatgac cctggacata
     7801 aacccttgcc ccgctgagcc cgagtctgtt caaaaaactg ccaggcagat catagctcag
     7861 cattgcagtc ctgagtttga tgtaatctcc gggtgaaaca tatctgctgt tgcgcgactg
     7921 attcccgtta acatatccaa gacgcgcctg gggtatgtct gtctgatcac ccggtttttt
     7981 ccagctatta agctggtctg ttgtctgatt gtcgaaccat gttgcattgg aggccagaaa
     8041 tgagtctccc gcaagatgaa ttttattgcc gcttacgccc tgaaacatga atgacaacac
     8101 aaaattcctg tagctcaggc tgttggttat tgcaaataaa acgggagggt taggattccc
     8161 cagaacaaca aatccggctt tggaataatc actggttgta gtggtatgat ctgtaatatt
     8221 tccctgggca tccctcgggt tcacatacca taatgcgtcc ccgttagccg ggtctgctcc
     8281 cgcatattct gccccgtaaa acactcccag gggctggcca accatggcca cattcataaa
     8341 taccgaactt ccctcatcga tcagtgagtt ttcgccaaga tcagtgatct tgttcctgtt
     8401 aacggaaaag ttgacgttcg ttgaccagag caatgctccg gcagtattgg tagtgttaag
     8461 gacgaattca aacccttcgt tcctcattga tcccaggttc tgtgcctgaa ccctgtttcc
     8521 tgatgttccg ggaacgggaa cattcaaaat aagatcggat gtatttttta tataataatc
     8581 gatcccgccc gagatcctgt tttcaaataa cccgaaatca agtccgacat tcgtctgaag
     8641 cgtagattcc cattttaaat tctcatttgg tacctgacct ggaatcaatc ctgagcttcc
     8701 attgtaatta tctgtaccaa acaaacctat atagctgtaa ttacctatac cggtattgcc
     8761 cgtaaggcca gtgcttaccc tcagcttcaa taagctcagt gcagccgcat cggcaaggaa
     8821 gccttccctg ctcagcaccc atccggcaga agcagcagga aatgttccgt atctcgactt
     8881 ttttccaaaa cgtgaagatc cgtcgacgcg tgccgacagg cttataatgt atttatacct
     8941 gtaatcgaaa tttatccttg aaaaccagga aagataactg tattccgccc tttcggatga
     9001 gctgccgctt attaaacctg cgtttgaaag agtccggagg tcatcagtcg gaaaatcctg
     9061 gccagtgagc catgcattgt caataacggt atactggtat tccgtaccca ggacaccgct
     9121 tatgccgaaa ttatcagatc tgcccgaata atctatgtat gacctgggtg aaatattccg
     9181 gatttcaccg taatttgcaa atccatatcc gcccgtatct tctcccgatt ctgtcctttt
     9241 cccgtaactg cagttctcct tcagatttaa aagatctatg ccaatctcgt ttttccagac
     9301 aagattctcc agtattctga aattcaggta gctgttcgcc agggttctca ggtttgagat
     9361 tttcctggaa gcatcttccg tatcaatcag gctgttgtaa taagttgtga tcggggtatt
     9421 gtacaatttg ccctttttat cccgcaccgg ggttatcgga gcaagggcaa ccagctgcat
     9481 gggtgatgaa aaggaattat ctgccggtat ctgatcaaca ttcgtacccg aaaggctcag
     9541 ggagactccg atgtcgagga acctgttaac cttgttgtca aggttcagcc ggcccgtaat
     9601 ccgctcccat ccgtttgata tcagtatccc ttccctgctt gtatatgaac cgcttgcaaa
     9661 gaatctcaga ttatcattgc cgccccgggc agaaaaatca gcggtcagga ggcttccagt
     9721 ccggaacgcc tggtcctgcc agtcagtatt tacggcaaga ttcggatcat tgtatgcagc
     9781 ccagccggaa tatcgttcaa accggcttct tgcaaattca agccacgatc cggaatagtc
     9841 ggcaggattg ttcacaggat caaggccctc gtaccggtcg ctgttgtagg cagcctcttc
     9901 aaagtaacgg atatattgtt ccgaattaag aaaattcctc ctggctgaag gctggctgaa
     9961 acctctctgc agtgtgaagt taagttttgt atcaccggaa ataccccttt ttgtggtgat
    10021 cagcacaacc ccgtttgccc ccctcgaacc gtagattgct gctgcagatc cgtctttcag
    10081 aatttcaacc gactctatgt cgttgatatt gaacgggagc aaaggattta tagctgcacc
    10141 tgactgatta agtgattctg tggcaagcgg aataccatca accacaaaca gcggatcatt
    10201 accagcagtg accgaggtgg cacctctgat cctcatcctt gccgaacccg ctaccttccc
    10261 gctcagcgac tcaatgaaga ccccggcaac tcttccctgt aacgccattt caactgtggg
    10321 cacaggcgct gaactgatac tggctccgtc taccctgact atacttccgg taaccagcga
    10381 cttttcccgg atgccgtatc cggtcatcac tgtatcattc tgacctggct ggtctgagac
    10441 aggtttggca ttatctgcat cgtcggatcc ggtgatgaca cctgtggttt tcatatcctc
    10501 agcagaaaac tccggccctg atgcctcccc tgcatcaatg ctgttcatcc aatccgtttc
    10561 agaaaacatc atattgcccg gatcactgag cagaacggcc cgcacagcag gttcattttc
    10621 tgctgtacca gctgctccgg taatgatctt ttcatgagcg gacagcaggc ccggataaaa
    10681 gcacgaaata aaaatcagca gatataactt attcatgatc attttttaag atttgaaatg
    10741 gggaaagatg ccggaacaga tcccggtcac tttcaaaatt ctgcagaaaa ggagccggac
    10801 tgcagcaatc atatattttc cactgctgac atgtctgagg ttacctgttc cgactgccct
    10861 gaaaatacat gacagaaaaa cataaaaaac aagcgccgtg agaatcatag tccggaatat
    10921 cctcctggtg ttttaaatta cctgtgtaat aagttttttc agacaaattc aaaaaagagt
    10981 ctgaagtatt gcaataagaa cgcaaatgaa tcaaaaaaat ttcaatttcg ccattccatt
    11041 cctgctttcc cccttccgta tctcaaggct gttcagccag gcaatatcaa taaccgggcc
    11101 ttgtgtccgg cggaaacttc agcctgatac ctcacggatc ggaagctgaa tactatattt
    11161 atcatatttt acttcccggg ataaaccaat atccctgcag ggataatcca attttgagta
    11221 tttttgtcat tggtcaaatt ctcctattca gcctcatcag atgacagcct tttcacatct
    11281 tcacgttcac acccagtatt ccattcttga cggtgcatcg catattccga acctcattga
    11341 aagggtgaaa tcgctgggca tggaagctgt tgcaatcacc gaccacggta atatgtttgg
    11401 tgtgaaggaa tttcattctg cagcaaccag gaagggaatt aagccgataa taggctgcga
    11461 ggtatatgtt gccaaaagat cgattggtga aacagcagga aaggaggacc ggtcaggtga
    11521 tcatcttatc cttctagcca aaaatctgac aggttacaag aatcttgtga agcttgtttc
    11581 aacatcatgg ataaagggtt tttactacac acctcgcatt gacaaggacc tcctgaagaa
    11641 acacagtgag ggtttaattg cttcatctgc atgcattgca ggcgagatcc aggatgagat
    11701 cctgaacggc agcatggaaa gggccgaagc cgtcctccgt gatttcctgg acatattcgg
    11761 agatgatttc tatcttgaga tacagaggca tgaaaccaat gatccggaag cagacaggac
    11821 ggtttttccc aaacagcagc aggtcattga ggctttcaga aagctgtcgg tcaagtacaa
    11881 tgtgaagatc attgcaacca acgatgttca tttcgtaaat gcagaagatg ccgaagcaca
    11941 cgacaggctg atctgtatta atacagcaaa ggatttcgat gatcccgaca ggctccgcta
    12001 ttcaaagcag gagtatctta agagtgagga ggaaatgcgc agaatattca gcgatattcc
    12061 cgaagcaatc gataacgttg gggagctggt ggccggaatt gagaaataca agctcgatca
    12121 tgatcccata atgcctgatt ttcagatacc agatggattc agtgacaaga acggttacct
    12181 caggcacctg acttatgcag gagcagcgga aagatggggc aatccgacca gggaacagac
    12241 agaaaggatt gattttgaac tggagacaat tgcaaaaatg ggctatccgg gatactttct
    12301 catagtccag gacctgctca gggctgcccg cgaaatggga gtatcggtag gtccgggaag
    12361 aggatccgca gcaggctcgg ccgttgcata ctgtctcagg atcaccgata ttgacccgat
    12421 aaaatacggc ctgctgtttg aaagattcct gaatctcgac cgcatctcca tgcccgatat
    12481 cgacatagat tttgatgaag acggcaggga agatgtttta agatatgttg taaataaata
    12541 tggccacgac aaggttgccc atatcatcac cttcggaaca atggctgcaa aaatggcaat
    12601 cagggatgtt gcacgggtcc agaggctgcc cctggccgaa gccgaccggc ttgccaaact
    12661 agtgccggaa agaccgggag tgaccttatc cgcagcctac actgaagtgc ccgagcttgc
    12721 aaaggaaaaa gactctccca acaaactggt gtcacagacc ctgaaatacg ccgaggtcct
    12781 ggaaggatca atccggcaga ctggcgtcca cgcatgcgga ataataatcg gcagggatgc
    12841 cctcgataat tatatccccc tttgcactgc aaaggatacc gacctgtacg ccacacaata
    12901 cgatggaagt catgtggaat cggtgggtct ccttaaaatg gacttcctgg ggctgaagac
    12961 cctttcaata ataaaagacg ctgttgagaa cataaaaaga tcacggggaa ccgtaatcaa
    13021 tattgaaaaa ctgcctctcg acgataaaaa aacattcgaa cttttcagca atggtgaaac
    13081 cacgggaata ttccagtttg aatcaaccgg aatgaaaagg tatctccggg aactcaggcc
    13141 caaccggttc gaggatctca ttgcaatgaa tgccctttac cgccccggcc cgatggagta
    13201 catccccaag ttcatcagga ttaaacatgg taccgaacca atcaacttca cccttccggt
    13261 gatggagagg tatctgaatg aaacctatgg gatcacagta taccaggaac aggtaatgct
    13321 cctctcccag gaactggcag gattcacaaa gggtgaagcc gataccctga gaaaggctat
    13381 gggaaagaag aaaaagtcca tcatggatga aatgaagctg aagtttttcg agggatgcat
    13441 caggagaggc tatgatgaga cgattgtcaa caagatatgg tccgactggg aagcctttgc
    13501 acaatatgca ttcaataaat cacattccac ctgctatgca ctgatcgcgt tccagaccgg
    13561 atacctgaaa gccaattacc ctgctgaata catggcagct gttctcagcc ggaatataag
    13621 cgacatcaag aaaataacca tcttcatgga tgagtgcaga agaatgggaa tggaagtgct
    13681 gggccccgat gtaaatgaaa gcaatgtcaa gtttaccgtc aaccgcgacg gtaatatcag
    13741 gttcggcctg ggtgccataa agggcgttgg cgaaaatgcc gtcctccagc tgattgagga
    13801 aagggaaaag aacggtgttt ataaaagtat atacgatctg gcagaaaggg tgaacctgaa
    13861 ttccctcaac aagaaaaacc ttgaagcaat ggcagtcgcc ggtgccttcg actgtttcac
    13921 cgacatatca aggtcgcagt attttgccat cgacacccgg ggagtcagct tcattgaaaa
    13981 cctgatcaga tacggcaata atgcaaagag cattaaaaac tcgagccagc agacactttt
    14041 tggatcaacc ggtggattcg acataatcaa gccggatgca ccatcctgcc ccgactggcc
    14101 caaacttgaa aaactgaaca aggaaaagga agtgatagga atctatcttt cctcccaccc
    14161 gctcgacgat ttcaaacttg aaataaacaa cttcaccaat gccaccctgg cagacctcca
    14221 gaacctcagt gaatggtacg agagagatgt aattgttgca gggatggtta ccgattcaaa
    14281 gaacgggata ggaaaaaacg gtaaacccta tgggtcattc accctgcagg attattccga
    14341 ttcattcagg ttcgtgatgt tcgacaagga atacatcgaa ttcagcaagt tcttcattac
    14401 aggatattac cttcttgtaa aaggaagggt gcaaaaacac aaattcagaa aggatgagct
    14461 tgaattcagc attaaatcca ttaaccttct ttccagtgta aaggatgaac ttgtaaagtc
    14521 ggttacggtg aaaatcaaca tggaaatcgt aaatatggca atgatcagcg atcttcttga
    14581 actgatgaaa gagaacaggg gcgaatcaga attgaagttt ttgttttacg accaggaaga
    14641 taaaatatcc ctttcaatgt tctcaagaac aataaggatc aggctgaaca atgaattaat
    14701 ttcatttctc gacgattacc cgggaatcga atataaagtt aattaactat atttgcgtcc
    14761 gtgagaatct gattataaat cataatggca tggcacaaga tgtaaacgac agcaacttcg
    14821 atgaaatagt aattaattcc gataaagtgg tactggtgga tttctgggcg gagtggtgcg
    14881 gaccatgcag gatgattaca cccattgttg aggacatatc aaaggaatat gccgacaggg
    14941 ttgtggttgc caaatgcgat gtggacgaaa gcccgggagt ggctgcaagg ttcgggataa
    15001 ggaatattcc cacggtactt tactttaaaa atggcgcagt ggctgacaag caggtgggtg
    15061 cagttccaaa ggcgaacttt gtaaaaaagc tggatatact tcttcaatag gagattccgg
    15121 atatctctta cagtctagaa gacatctctt tgatgcagga agtcgcctga gcggcgcaaa
    15181 acccatacgg aagtatcagc ggaatacccg cttctgaaat cgcgacataa ttaatccggc
    15241 agattattgc ctgattacag cctcaaaact gacaaaacca actctcacaa gtcctgtttt
    15301 taaaataatt tcatgacagt ctgcgtgttt gctgcttcaa gcagccggat cgacaaaaaa
    15361 tatgcggagg cagccaggga gctgggaact ctcattgcca gttcgcgaat acaggttatt
    15421 tacggcggag gaggcatagg actgatgggc gtgcttgccg acgcggtaat ttccggcggc
    15481 gggataatta cgggtatcat gccttcattc atgaatgatg aaggctgggg acatccccgg
    15541 gtaatggaca tgatacttac atcagatatg gctgaaagaa aaaaaaacat gttctcaagg
    15601 gccgatgcgg ttgtggcact cccgggcgga gtgggaacgc tggaagaact aaccgaggca
    15661 atcaccctga aacagcttgg tctgttcagg ggtcccgtgg ttatcctgaa caccctcggc
    15721 ttctacaaat cacttatcgg tttctttgaa cacatgatca gcaatcattt tctcaggccg
    15781 gaacacaggg gaatatggac agtggcagac acccctggag aggtgataag agcccttcgg
    15841 aacaacacca ggtggattga tgatccgaga agaattgcca ggatctagca ttgagtgaat
    15901 attgtttata ttgagatatg tcaatttact aattgatata tctcaatgtt gcttaaggta
    15961 ttgatcaacc gtatctgctt ccggtaacta tttccctcag ccaggtcaat ttcatttgtt
    16021 gttcagcaac cttttcagtg tactgttgct gaaagttttt tccattatgg tctttccttc
    16081 aaccgaaggg gctttcaccc cggctttgaa ggcctttgta ccggtaaata cacaggcttc
    16141 gtcccagtaa ccggttttta taaaatgact aaggacctgg gccccgcctt cgatgaacaa
    16201 cgactggata ccccagccgt gaagcgcctc agatatctgt tttgcggcag gttcgcgatg
    16261 gttaagcttc ataatcactg agcccggaag taccactttc tccggataga atgtaaaaac
    16321 aatcaatgat ccgattgatc tgttcactgc gggatcatca ggaagtgaac ctgatccgct
    16381 cagaataatg cgaattgggt tgctgccctt ccattccctc acattcagcc ggggatcatc
    16441 ggccctgacg gttcctgccc cgaccaggat tgcctgctcc gaagctctcc acttgtggac
    16501 aagtatcctc tcggcccggc cagtgatcca gtttggctca atcctgtagt cttcatccct
    16561 ttccctgtcg atgaatccgt cgctgctttc ggcccatttc agtgttatat agggtctttt
    16621 cttctcatga aaggtcagaa accttctgtt aattctcctg cattcatcct cgcaaacacc
    16681 ggtaacaacc tcgcatcctg catcgcgaag cctggaaata ccctttccgg acactttggc
    16741 acttgtatcg atggtcccga ctaccacttt tggtatcttc cacgatacta tcatatctgc
    16801 gcacggaggt gttttcccga aatgcgaaca gggttcaaga ttgacataaa gggtcgattc
    16861 ttccagcagc tttttgttac ggactgattc caccgccctg gattcagcat ggggtcctcc
    16921 ggctttaata tgatatcctt cccctatgat gtttccctga tgcacgatca cggccccgac
    16981 catcggatta ggataagtca taccctcagc cttgctggcc aggtcgaggc atctgcgcat
    17041 gaatttcatg tcattatttt caggcatttg cttgaaagtg ctaattttgg ctaatggcct
    17101 tcaatttaca aacaattaga gatatccgga accatatctc gggagaactt aaagaaatat
    17161 atcctgagga agaaatccag tcgctttttg aaattatggt actgcatata ctgggaggga
    17221 gcaaggccgg tctgctcggg aatcctcaaa gacggctgac aaatgaagaa tcggaaagaa
    17281 tcatgttgtt ctgcaatgag ctcaaaaccg gcaggccggt acagtacata acaggggaaa
    17341 cagttttcta caattgccgc atcagggtcg gaccggaggt attgataccc aggcccgaga
    17401 ctgaagagct tgtggatctg gtgttaaggg agaaccggaa tttttcaggt acaataaccg
    17461 atgcctgtac cggttcggga tgcattgcta tcgcgcttgc ccggaacctt ccggcagcag
    17521 ggatcacagg tctggacaat acccccgcgg cgttaaaaac tgcatcggga aatgccctcc
    17581 tgaacagggt cagcattaac ttcatactgt cggatattct gaagaacggt attgccgatg
    17641 ctccgaagcc ggatattatc gtgagcaatc cgccctatgt aagggagagt gagaaggccc
    17701 tgatgcacag gaatgtgctg gagtttgaac cgcacagtgc actgttcgtt cccgatgatg
    17761 atccgctggt cttttatcgt gcaattcttg agtcgggtaa cagggtaatg gatcggggag
    17821 gaaaaatcta tttcgaaatc aacgaagcta tgggtgaatc catggtcaat cttatggaat
    17881 cacttaatta catcggtatc agggtagtaa gtgatattaa cggcagggac cggataataa
    17941 aaggaacact gaaatgaata aaaagaacgg gctgaaaacg gcgctgaaca gagccatggc
    18001 cctgtgcgca tcacgcgaat actgcagtgc agatatccgt tcgaaactgg aatcatgggg
    18061 catctgcacc accgaggccg aaaagataat ctccgggctg atcagggaga agtttcttga
    18121 cgattcccgt tatgcccatg ctttcgtcag ggataagttt aaatataata aatggggcag
    18181 gatgaagatc gcagccggac tgagacagaa aaaaatcgcc gaggaaataa tccgcaatgc
    18241 gcttggggaa attgatgaag cccggtatat ggacatgatc agggaaaccc tgaacttaca
    18301 ccgcaaatct gtcagggcaa agaaccagta tgagctcagg gggaaattat taagatacgg
    18361 actgtcgaaa ggtttcgaga accattttct ttatgacata ctgaatgatc tggatccata
    18421 aatcaggaaa tgcatactgt aaatccgcca tccggaaaga atctcaggat tcttataata
    18481 aaccgtgttc ctgaaataat aatttcctgt ggcacaacca cggatccccg taggagtatt
    18541 ttgcatccgg atgacaatct gccatctgca gacatggatc tgtacggaag aaagctttgt
    18601 cagcgattgt atcaacagtg cagtcaaagg ggttatttac cgggcatgac attcgccggt
    18661 caatagcggg ttcattgcta atgaatgccc gacaggctga agatattgat gaggaatggg
    18721 aaaaatgcca ccaggaacaa cacggttgat ccggatatgt catatatgat cagctctttc
    18781 ccggttgagg gatattttac cctgcaggct gaaaacagta tcctcatgat cagctgggcg
    18841 gcaaagaaga tgaatatggc cggaccattg gcattccatg tacgggcttc cgcaattctt
    18901 ccccttatca tcagggaaag gctgtgcgac aatccgcagg atatacatgg ttgtcccgta
    18961 actttttcat gtatgcagac tacaggataa ttgtcttttc cgggtgaaaa tataccggaa
    19021 taaataaaaa caagcagaat tacggtggcc agaaccaggt ttataacaag ataggaatcc
    19081 ctgtccgcag ggacatatct gctgattgcc accccggtca ttttttaacg tttgtcccgg
    19141 aattcaggga atcggttctt tccagctcct caagctcctc aatccggctt ctgctgcctg
    19201 tatctgcttt tatctccacc ttatccccgt tgctttcgac cttgatcgag atttttgcat
    19261 cctcaaggtc ctgcagtaca tctttctgta catccctgat aatgttctca atatcgccgc
    19321 cgaagcctct ttttgccttt tctataatct ttccggcaac aaacaactgg gaaaatgaga
    19381 ttattgaagc aaccacggca ataaccagtc ctgcgataag cacaccccgc gattcgcttt
    19441 ccgatgcgcg tgaaagaccc accactgcaa ggacaattgc aatcaccccc ggtattattg
    19501 caataagtcc gacacaaggt atcaccgcga acacaaatgt cactatggcg gtaataagtg
    19561 cagcaattcc cagattctgt ccggtccgtg aacctgtata tccagcacca ggtgatgaac
    19621 ctgctgatcc ctgcccggta ttgttaatct gatcttccat gtctacaaga tgttttatgc
    19681 gttattgttt tcctgtcaaa atattcagca aatcctgttc taaagacgtc accggattct
    19741 caatgatgcg tccgagttca actttttttt cataaacgat aaaagtcggc acgcgttcta
    19801 ttccaagctt cccgtaatcc cccaacggtg acagtttttc attgtccaca ccgataaatg
    19861 tcagctgccg gacgggaaaa ccgatcgaat caatgatttt catgaaacgg ggtacctctc
    19921 tcctgctgtc ggggcaccat attccgagca caatcacgat cgtcagatcg ttgttcccca
    19981 gggaagaaag tttttccaca acatcgtgac ggggctgata tccttcatat tcactgtcat
    20041 accactccga atatggtggt cgcagaagga gtgagggact gaagtagccc agaagccaga
    20101 ccgattgttc cgcccagttg gtggaagatg ttccctgctg tgttgccact ccgggatcac
    20161 cattatttcc tttgctcttc tttccggtcc cgcagccaca gctgatcagc agtaaaatga
    20221 aaaggacaag tccctttgct gatatccatg gtaatatttt catatgctaa tcaataagtt
    20281 ccgaagtttc ctgtaccctg taacttcttt aatcgctgtt atggagcaat atttcaagaa
    20341 tctgcctggc agcttttgaa acaggggttc ccggaccaaa aatggctgcc actccagcat
    20401 cgtaaaggaa ctgataatcc cccggaggaa tgattccccc ggcaactaca agaatatctt
    20461 cccttcccag ttttttcaat tcaaggataa tttgcggaac aagggtcctg tgacctgctg
    20521 caaggcttga tacacccaga acatggacat cattttcaac tgcctgtctc gcagcctcgg
    20581 caggagtctg gaacaacggg cctatatcca catcaaatcc gatatcggca tagcctgtcg
    20641 atactaccct ggctccgcgg tcgtgtccat cctgtccggt cttagcgatc aatatcctgg
    20701 gctggcgtcc ctctttcgca gcaaattcct caaccaggac cctggccttg gtgaactctt
    20761 catctgactt atactcagaa gaataaacgc ctgaaattgt tcttatcact gctttgtacc
    20821 tccctgttat tttttcacaa gcaaaagtta tttcgccgag actcgccctt tttgcagcag
    20881 cctcgacggc gagttcgaga aggtttccct tcccgctgcg tacacactct gttatggcat
    20941 cgagcgatct catgcactca tcctcattcc ttcccgcctt gagcatttcg agtctcttaa
    21001 tctgcgcccg gcgtaccatg ctattgtcaa cttcaaggat ctcaacaggt tcttcattgt
    21061 ccggtttgta tttgtttatg ccgacaattg tctgaactcc cgagtcaata cgtgcctgga
    21121 ccctggttgc agcttcttca attctcattt tcggaagccc cgtctcaatt gcctttgtca
    21181 ttccgcccat gctttcaatc tccccgatat gcttccatgc tgagcgggcc agtgcatcag
    21241 taaggctttc aacgtagtat gagcctgccc acggatcaat tgccctgcag atgtcagttt
    21301 cccgctgaag atagagctgg gtattcctgg caattgcggc agaaaaatcg ttcggaaggg
    21361 caagggcttc atcaaaggca tttgtgtgaa gactctgggt attcccgaga actgcagcca
    21421 tggcctcgat acaggtcctt cccacattat tgaaaggatc ctgagccgta aggctccagc
    21481 cggaagtctg gcaatgggta cgcagtgcaa gagacttagg attcagaggt ccaaaagtat
    21541 tgacaatctt tgcccagagc agtctggcgg cccgtaattt ggcaatctcc atgaaatgat
    21601 tcatgcccgt tgcccagaaa aacgagatcc tcggtgcaaa atcatcgata ttgagcccgg
    21661 cctttattcc cgttcgcagg tattcgaatc cgttggccag agtataagcc agttccaggt
    21721 cgcatgaggc accggcctcc tgcatatgat accccgaaat gctgatggaa ttgaatttcg
    21781 gcattttttc ggaagtatag cggaggatat cggcaacgat cctcatggaa aattcaggag
    21841 ggtagatgta ggtgttcctg accatgaatt ccttgaggat atcattctgg attgtcccgg
    21901 aaagctcatc gagcctggct ccctgttcca gggccgttac tatgtagaat gccattatgg
    21961 gaagaacggc gccgttcatt gtcatcgaaa ccgacatcct gtcaaggggg atccggtcga
    22021 aaagaatctt catatccaga accgagtcga cggcaacccc ggcacttccc acatcacctg
    22081 ccacacgttc atgatcggaa tcatatcccc tgtgcgtcgg cagatcaaaa gccaccgaca
    22141 ggcctttctg ccccgcagcc agattcctgc gataaaaggc attcgagtcc tcagccgtgg
    22201 aaaaaccggc atactgcctg atcgtccacg gacgtacgat atacattgaa gcatacggtc
    22261 cgcgcaagaa tggcggaatg ccggcagcat aatccatatg ttcaatccct gaaagatcag
    22321 cttttgagta aaaagccttc agcggtatct gttccgccgt aattatgggc tccgcctttc
    22381 tttctgacat caccctttca tctgcagatc cgcttgaaaa acagttgtgc agatcatcaa
    22441 aatagaattc cggcctcatg ataatttata gttttctccc ttccggtcac cagctttttc
    22501 tccgtgatcc ttccggtcat gaagcaaagt atgtatcata ccggaattca acgcctgaag
    22561 aaatcctccc tttttctcaa tctggaggaa gatgttccac gcattttcag caatgtggga
    22621 agtaatggtc tcaatataat acgatccggc agaaggatcg gcaaccctgt caaaaaaagc
    22681 ctcttctttc agcagcagtt gctgattcct tgcaatccgc tccgggaaat cccctgtcat
    22741 gccattcctg tattcaacgg ggtgaaccgt aagggagtcg gtacctccga taattgccga
    22801 catggcggct gcctgggatc cgatcatatt caggaacgga tctccagcag cagcattcag
    22861 gactgtttca gagtggatct ccatccttgc aggaaaatca tccgtaacgc cgaattttct
    22921 gcaaacaacc tcccagagca gacgggcggc cctcagcctg ccaatttcca taaaataaga
    22981 cggccctatg ccaaaactga aacgaatcgc actggctgcc gtaccaggat caatacccct
    23041 tgatgtcagc tgatcaatgt attcaaccgc cattgcaatg gcaagagcca gttcaagcac
    23101 cggacccgac ccggccttcc tgaagtatgt tccatgtata tggatcgaac ggtaataagg
    23161 aaggtccatg gctttacgga ccagtgcagc gagatagtca agtccggcgt ctacaggaat
    23221 gcaaagtttt ccctgaatca taagccggac caggggatct gcttcaacgg cgcctctcac
    23281 cgatttccga cccggttcct tatcagccag taaatcactg acaagcgaaa ctatttcaat
    23341 cgctttgcct tcggaagaaa aattaatttc tgcacatgaa ggattaaaat ctctgagaag
    23401 catagcaaaa ttttcattgc tgattgtgtc aggatcacct atcagaaatc caagggaatc
    23461 aactcccctt tccaggagag agagggcctg ctcattcgca gacgcataac tctgaaccaa
    23521 gacatcctgc cggacaagcc aggatgccgc tttatcccct ttactttttc ctagttgtcc
    23581 cggatcctcc ttgctattac ggcattttaa cccatccttt ccaagataca acggcataaa
    23641 ggcatctgtg taacgcagct tttccatgtc ttcccgccgg tagaacggca tcagatcaaa
    23701 gccttctccg gttttccata gaagtttctc atttaaatcg gctcctttca gttcggccct
    23761 gatcctatcg agccattcgc ctgtactcgt ggcaggaaat tcttcaaata actttttctg
    23821 tttcacccgg atgctgtttg catttataat aatggatttt cattttcccg ttccgtatca
    23881 tgagagtcga accctcaggg tttcaggtgc gtacaacaac agctattcct gattaagttc
    23941 agtctgctgt cagtttccgc caaattccat caggtatgac ctgataaatg aattcaggtc
    24001 accgtcaaga actgccataa catctcccga ttccttattg gttctgtggt ccttaaccat
    24061 tttgtacgga tgaatggtgt atgaccttat ctgcgaaccc cattcgatct tcttcttgct
    24121 gccctcgatc ctggcctgtt cttccctcag ctttctcaat tcgagttcat aaagctgcga
    24181 ccttagtatc ctcattgcat tttccttgtt gttgagctgg gatcttgtct cctggttctc
    24241 aataacaatt cctgtcggat gatgtcttag cctcacggcg gtttcaacct tgttcacgtt
    24301 ctgtcctcct cttccgctcg accggaatgt ttcccatgtg atatctgcag gattgatttc
    24361 aatttcgatg ttgttgtcga caagcgggga gacaaaaaca gaagcaaagg tggtctggcg
    24421 ttttccctgt gcattgaagg gggagattct cacaagccgg tgcactccgt tctcgctttt
    24481 cagatatccg tatgccctct caccatctat ttcaagtgtt actgacttta cccccacttc
    24541 atcccctgat tgaaaatcaa gctccctgac tttatagccg ttcctttcgg cccatctgag
    24601 atacattctc ataagcatcg acgcccagtc gttgctttca gtgccgccgg cacctgagtt
    24661 gatcttaagg attgccccca gctgatcttc atctttcctc agcatgttgc ggacttccag
    24721 atcctcaacc aatccgaggc tttgctcata ctggttatcc gcatcctcct cactggcctc
    24781 gccttccttg tagaaatcat acatcacctg aagatcgtca aggcttttgg caaccttctc
    24841 aaattgtgta gtccagcttt ttatccgtga aatctcccgg agaagatcct ctgccgccct
    24901 gggatcgttc cagaaaccgg gtgcccgggt aagggcttcc ttttcagcaa tctgcaatag
    24961 tttcccatcc acgtcaaaga tacctcctca acgcctcgca gcgttctgaa agatctttta
    25021 tctgctcatt ggttaacatg atgaattaat tttccgcaaa tttattttaa atcaggttct
    25081 ttgcaaaata aactttcttt cccggaagaa agacaaacaa tggtttgggt atcagatccc
    25141 ggaaactggt ggtgtgcctc cggtttactc acccggtata tttcagtctc aggctgttgc
    25201 ttaccacgct tatactgctg agagccatgg ccgcgcccgc gatcatggga ttgagcagaa
    25261 agccgttcag cgggtataac acgcccgcgg ctatcgggat tccgataatg ttgtaaataa
    25321 aggcccagaa gaggttctgc ctgatggtcc tgaccgtgtt cctgctcagc ctgatggcgg
    25381 cagggattac agccagatcg ttgctgatta tggtcatcct ggcagcgtcg atggcaatat
    25441 ccgagccttt gcccatggct atgcttacat cggccctggc cagggcctgg ctgtcattga
    25501 tcccgtcgcc gaccatcccg acaatcctgt tttccttttg aagagcttca acatattctg
    25561 cctttgcagc cgggagcatt gacgctgcaa actccgttat tcccgccctg gcagcgacag
    25621 ccgcggctgt cttccggttg tcgcctgtaa ccatcctgac atctattcca agctttttaa
    25681 tttgtcccac ggcatcggcg gatgtctctt tcaggctatc gttaatcgat aaaactgcaa
    25741 gcacttcagt ctcgtccgcg aacatgacaa ctgtcctggc gttttcttcc caggtttctg
    25801 ccaggcccga aagccgcggg gcaacaggta tcttattttc actgatgaac ccctgtccgc
    25861 cggccaggta tttcctgccg ttatgtaaac cctcgatccc ttttcccgtg atgcttttaa
    25921 cgttctcaaa ccgggtctcc ggcatgttgt tatcaaaatg ccttacaatt gcttccgcca
    25981 gcggatgttc cgacaaacgt tccaacccgg ccagtatcgc tgcaagctct tccttgtctc
    26041 ccggagtttc ccattcaata ttttcaactg tcggtcggcc ttccgtgagg gttcccgttt
    26101 tatcaagtat cagggtgtcc attttgcagg ccagttcaag cgcttcggca tcacggataa
    26161 gaatattgtt ttcagctccc tttccgatcc ccaccattat ggccgtgggc gttgcaaggc
    26221 ccagggcaca ggggcaggct atcaccagca cggttatcat cgaaaggagt ccctgtacaa
    26281 acccgttttc cccgcccgcg atccaccaga ctgcaagtgt aatcactgcc aggacagtga
    26341 ccaccggcac gaatatacct gcaatccggt cagcaagttt ttgcacggga gccttgctcc
    26401 cctgtgcctc cttaacatat tttataatgc tgctgagcag ggtgtcggca ccaactttct
    26461 cggctgtgaa ccggaaagtt cctttctggt ttatcgtacc cgcgaacacc tttaagcctt
    26521 cctccttgta aacggcaacc ggctcgcccg ttatcatgct ttcatccacg taactgccgc
    26581 ccgaggtgac agtgccgtca accgggatct tttcacccgg cctgacgagg ataatatcgc
    26641 ccggtttgac ttttgaaata agaaactccc ttatctcgcc cgaaggaagg accttgaaca
    26701 ctgttttggg ctgaaggccc atcagctttt ttatggctga agaagtattt gccttcgctc
    26761 tctcttcaag aaatttcccg agcaggacaa gcgctatcac cactcccgag gcttcaaaat
    26821 aaatatgcgc ctggagcccc ctgctttccc agaaccccgg gaaaagcgtg ttgaacacac
    26881 tgaacagata agctatgccg gtgctaaggg ataccaatgt atccattgaa gtggcgccat
    26941 gaatgatctg cttcccggca ttgatgaaaa atgagcgccc gaacaagaag acgatgggag
    27001 ttgccagggc ccacattatg tagggggcat ggggaatatt catgaacacc atgcttatca
    27061 gcaccagggg aacagcaagt ataacggccc atattgtcct gcggcgcatc gtcctatatc
    27121 tttttagctg gagactctca agttcttcct ttgccgtttc cgactcgtcg gttatcaggt
    27181 cgtagccaat cgaggtgacc actgccttca gatcttccgg gcgaaccatg gaactgtcaa
    27241 actccaccgt tgccgcttca gaagcaaggt tcacgaacac attcagcacc ccgggctgct
    27301 tcccaagcat cgtttccacg ttcgctgaac aggaagcaca cgacatgcct gtcacaggat
    27361 attgcttctt tacaatcttc tgaactgtct cttttttttc ttttatagtg tctgtatctg
    27421 acatctttca atcaaaattg aatctcccgt atttctcctg aagccccggt aattaacagt
    27481 tgagccagat tatatcaccc gtcagcaacc gggatgcaaa attaaggata ttctgttgta
    27541 acaatgcaga agcgcttaag tttaccggga tggatggatt tcgctggtta taatgcaggg
    27601 aaattcatgg aaatgattct catcggcatt gacgcgacca cccggcgaaa aaagttgttt
    27661 cgttggtatt gccgccggta aaagctgatc atagcctctg acatgtcagg caaaagtaca
    27721 cggcacctcc caggtaagct tctttcctga tctgatcgcc gcacacccgg cacgggtgct
    27781 gaaatgtgtt cctggagaga atggttttat atcccccttc attcccgaaa aaatccttct
    27841 cggtatccct gcctcctttt tcggtcatta ttttaagtgt atccctgaca cttatgaaca
    27901 agtcggcttt gtccctaccg gagagagacg atttcttccg tttcggatgg attcctgcat
    27961 tgaaaaggat atcctgcaag accccgttcc cgagaccggg aaagcgttgt tcggtcgcta
    28021 gcagggcctt gacagaaata ttttccggcg aatcgttcag gatgacatca aaataatctt
    28081 catcaaatgc atcgtcaagg ggtgaaatgc tgttaagact cagctggtga tacctgtttt
    28141 caaaaatacc tctgtacgcc catatcccgc catacatcgc cacgttaaat gcaacatggc
    28201 tgttatcatc aaatgttatc aggagctggt atttcccggg aggttcttca gaaggagcat
    28261 gatacctcat aatggttcct tccccgattg taatgcagga ttccccgtcg caaaggatgt
    28321 caacgaacat cccgtgtcca aaggttgatt tcacggtttt gcctgagaga agcctggcat
    28381 attcagcggg atcgcctgaa aaccacgcga atttatgatg actggtcgga ctgaaaacat
    28441 ccgttattct cctgccggac agtatttttc cggcctgcag actgatagtt tttgattcgg
    28501 gtatctcgag catacaagtt taattagcaa tttgttaatc atttaacaaa tacttctgtt
    28561 ttaatttgat aaggtccgcc tgccccgggc aggtaatccg cgtgttgaaa atagcattta
    28621 cctatattgg cgaacggggt gtatgggaag ttcataacca attggccctg atgtgattta
    28681 ttcatcctgc ctaacagcaa atttcaacaa ttctccggaa agatacagca gcagcccgaa
    28741 aaaaagaaaa ggatcttccg tgaatgaatt acggcttctt atttacatgc ttactgaaac
    28801 tcagcaccag aaaggatgcc gcgacaaacg ggaaggtaag ctgagtgata ttgaaatgat
    28861 gcattataaa acttataagc agggaaagca ataccgaaat gaataccccg atcgcgtctt
    28921 ttaatttttt accggcgaat accatggcgc ataacacggc attgaaactg tacaaaccgc
    28981 tgccaactgg cccggcagga gctcctgaaa gtgttgcaag gacacccgat accaatgccc
    29041 cggcaatgcc ataaagcgcc gcgaccggtg agcttataaa cactgccagg aagaaaagga
    29101 ccgatgatat tacattctcc tgaaaaataa cctgcccgaa tcccctgatt gaatatgata
    29161 aaggattggc aacattaaca ggcaacgccc ccggggttgc cagcaagtca ggagcaaacc
    29221 tgccaaaaag cacgagagcc agccaggtaa ccaaaacaaa aggtaaggtg aaaacaggga
    29281 tgttcattct aagaaaaaag tgctgcatta ccgttgccag tacggagcct gctaccacag
    29341 ccatccatgt caccggagcc ggtttaaaga aaagcatcat ggccaccccg gtcagggaag
    29401 cactgaagcc gtacaggccc ttgttcaggt ttcccgtatc atatttcagc aaaacggcag
    29461 ttaacgttcc gctggttacg gccaccagtg ctgccagccc catcagtgcc gatccataaa
    29521 atattccagc caggaaaagc aacccggtca atgcattctc ctgaagcatt atctgtccaa
    29581 gccctttcag tatgaggttt aacagatgaa aatcaattcc ggggattacc ttttctttct
    29641 ggtgtttatt atccatacaa gattgagcag ggaaaaacct gaaacagctg acaaagttac
    29701 attaataatc atgtcaggga ttatttactt cctgagaatg aaaaacttta gctgctaaaa
    29761 acagcactcc tcacaaacta aatccctgaa tatacagatt ttatgaaaag gctgcatttc
    29821 cgggacagat gtttgtccac cactattgat acccgttatt tcggaagtgc cggcatgagg
    29881 cccagtatcc atctgacagt caggttaccc tggaaagccg ggacagttcc cccgatgata
    29941 tcctcgctca ttgtaacctt gtgaaaatca aaattcatgg ggctttcaac cggattctca
    30001 tttgtataag gaatatctat cggaaatatc catccacggt catcatccca cctccacaca
    30061 gtcattgtgc catcaatcag ggtgttggca ttgattataa cataaggaga cccatctata
    30121 gttttataac tgccacttaa tgatgttatg attatatcgc cggtttcacg taattttaca
    30181 gtgttaggaa tctcatctgt cgcatcaaaa tgctcagacg aaggctggct gattataata
    30241 tctcccgacc ggtataaatc aacatccata tcaatgatcg ctgaaaaagt cgggaatgag
    30301 cgggtatact caaacctgag ggtgcctttg taatctttat ccagttggtc attttcttct
    30361 ttctcttttg aacaggaaca aaacaaaaca atgagaaaaa ccacgggtag tattctcata
    30421 ccggaaaatg aaggcggtgt tttcatgttc ataatggtta aatttgtcct gattaattca
    30481 taaacataaa attaggcaat aaaattatca aatttaacct taaccagtgg tcttaattct
    30541 ggggagtata ataatgacat ataactccct gttaacctac aggctgacca ggcgggtgat
    30601 gatattaatc agccagcccg ggtagatccc taaaatcagg atacccagcg tgatcaggac
    30661 gagagccatg ttgccgttaa gtgatattgc cggcagcccg gcatcgtcac ccggagtgaa
    30721 catggaagtg ataacccgga ggtaatagta caagccgatc acgctgttta tgatcatcac
    30781 tatgataagc agcataagcc ccgccttcat accctcgagt atgagataaa acttagctat
    30841 gaacccggcg gtgaaaggga tgcctgccag ggagagcatt gaaagggtta atacaagtgc
    30901 tgcccatggc ctggtccaga acaggccccg gtaatcatca atcttttctg tttcctcacg
    30961 tgaaatggaa agaagggtaa taacgccaaa cgcgccaatc gtggttacaa aatatgaaat
    31021 caggtaaaaa accgctgcct gtattccttt atccgttcct gtcagcagga gtaccaggag
    31081 atagcccatg ttggcaatgg aagagtaggc aagcagcctt tttatgtttt gctgcttcag
    31141 ggcgagtaaa ttcccgatga acatcgatag tatggccaat gccgaaatca cgacgaaaaa
    31201 aaagcgggag tcatatcccc tgatatcgaa aaagaacctg atcagtacag ccatgaccgc
    31261 gcccttcgac acggttgcaa tataggcgct cacgggcgcc ggtgctccct ggtatacatc
    31321 aggggtccac atgtgaaaag gaacaagggc cagtttgaac cctataccga ccatcatcat
    31381 tgaaaaaccg accgacacga aaccggctat cgcatcgctt cccttcagca gggaggctac
    31441 gccggcaaac tgcattgtcc cggttccggt gtaaatcaat gccatcccga ataacaggaa
    31501 cgctgatgac accgatgcca gaagaaggta tttaatacct gcctctaccg aagtgtcttt
    31561 tgaccgttga aaagccacca ggatatacag cgagacactc agcagttcaa gccccaggaa
    31621 gaaaatcacg aagtgcccgg cgacggcaag cagtgaagcg ccaagcgtgg aagtaaaaag
    31681 cgtcaggaaa tattcttcct tcactccccc gagagtgccg atatagtcat gggaaagtat
    31741 ggtgacaagc aaagccgaag caataattat acccaggaaa aaaaggctgt aaacgtccac
    31801 gatgagcaag ggttcgatgg aacgcggtaa aaacgagccg gtaaagaaca gggacgcgaa
    31861 agcaacaata aaagccagaa ccgaaaagcc ataaataacc tcgtaattcc tcaggatcga
    31921 aataacgatc atcatcacga ccggcgccat ggcgataaca atgaggggca tcatggaata
    31981 aagatcagct atggtcatat ctcagtgctt tttaaacatt cctgacaact ggttgaacga
    32041 ggctgttttc ccgttcccgg cactcttgcc tgagtctggc agcccggtca gctgagcggg
    32101 atgagaatcc agcgtcttaa taatagccgg accggccagg ttaagtaccg gccgcgggaa
    32161 aagaccgagt gcaacaacca gtacaacgag aacagcagaa acgatcttct ccctcaggtt
    32221 caggtctgca atctcccatt catgattcat cttcccgaaa aacactttct gcacgatccg
    32281 taaggaatag attgtagccg ccacgagtcc gaggcaggcc aggctggcca tcagcacact
    32341 ggctttaaat gttcccagca gggttaaaag ttcggcgaca aaattcgcca ggccgggcag
    32401 cccgagtgat gccagggaga aaatcatgcc tatggctccc atcaccggtg ttttttccca
    32461 gaggcctccc atctggtcca tatccctggt atggatacgt tcatacagct gtccggcaag
    32521 aataaacaat gccccggtgc ttattgcatg agctatcatc tgcatcacga cgccctggta
    32581 cgcgatcacg ttgaaggcaa atactccaag gataacgaaa cccatgtgac tcacgctggt
    32641 ataggctacc agccgcttga ggtccgtctg tgcaaaggca agcttggcgc cgtaaaggat
    32701 accgataacc cccagtccca tccctacagg ggcaaaagca gcggatgccg atggaaacaa
    32761 cgggacgaca aaacgtatca acccgtatgc cccggtttta aggagcaggg ccgcgagtat
    32821 gacgctacca gcagtcgggg cctggctgtg ggcatcaggc agccaattgt gcaggggaac
    32881 aaccggcagt ttaaccagga aagcggcaag gaaacccgac attatcaaca gttccttcgc
    32941 cgccggcatc tcaagcccta aaagctgaac ataatcaaat gttaatgtgc ctgaagtgct
    33001 ttcatgaaca aaaacaagag ccagtatggc aataaacata agaaggccgc tggcctgggt
    33061 atacaggaaa aacttgtatg aagctgctgt cctgttctcg tgaccccata tccctatcat
    33121 gaaatacatg gggaccagca tcatctccca gaaaaaataa aacaggaaca ggtcgaggga
    33181 caggaacact ccgatgatcc ccgccagtat cagaagcagg ttgaaatgaa agaacccgac
    33241 cctggaatta atttccttcc aggaaatcac gaccgatatg ataccaagga acaatgtcag
    33301 caataccatt acaagactta gcccgtcaag cgccaggtga aggctgatgc caaaccgggg
    33361 tatccattcg agttgcaggt ccatcagcca tttgccgtca gtgcctgata cactggaact
    33421 gaaaattatc agcagtacca gatcaaggga aacggcagcg acggaaataa cgcgcggcca
    33481 gaggttaccc tttctcccgg tcaggagcgc gagcagtgac cctgccagca ggataagtat
    33541 aaggtatgcc agtatcatct caatatcatg tatgtcagga tcaatactat accgattgta
    33601 aaggacatca cgtaccatcg cagcttcccg ttctgggtaa ggctgaaaag acggttcccc
    33661 agaacagtca ggcgtgcaag accaatatta agccagtcaa atatgtcatt cctgttaatt
    33721 gccgacagcc acaccagcgg gtttaccagc aacacgtcgt aaaggcggtc gaagccgaac
    33781 ccgctctcga aaaagcgatg aatacggccg tgttcaaatg attccgacat cgagggcttt
    33841 ttgtagtaag ccaaataagc caggtaaatc cctgcaaaca ctatcaatgc tgacaaaagc
    33901 tggaacagtg tctcggggta accggtttcc cttaccgtga ccgctggtaa agtattctcc
    33961 gcgagagagg aaaaaagatg aaagcgcccg aatgattcag gcagctcgat aaaacccgcc
    34021 acgattgaga agaaggccag tattgccagg ggcactgtca tcagtttacc cggctggaat
    34081 gagggtttta attttgtttc accaaaaaac acgacaaaaa ccagcctgaa ggtatagagt
    34141 gttgtgagga aggccccggt gaaagctgct atccacaacc agaaatttcc agtgactgaa
    34201 gaaaacgagt accacagtat aggatcctta ctgtagaacc ctgctgttac caggggaagg
    34261 gcagacaggg acgcggagcc ggcaaggaaa gtaaaaaaga tcagcggcat ttcttttttc
    34321 atcccgccca tcctgaaaat atcatgttct tctttaagca ccagtataac cacgcctccg
    34381 gcgaggaaga ggagtgcctt gaagaaggca tgtatcatga agtggaatat tgccgccgac
    34441 catgctccca ctcccagggc gagaaacatg tacccgatct ggcttatggt ggaatacgcc
    34501 aggattcgtt tcaggtcatg ctgacccagg gcgctgaagc cagcgatcag caatgtcagg
    34561 gcgcctataa tggcgatggc tgtcatcaca accggggcaa ggttgaagag cacgtggcag
    34621 cgtgcaatca ggtacactcc cgctgtcacc atggtggctg catgtataag ggcgctcact
    34681 ggcgacgggc ctgccatggc atcgggcagc caggtttgca gcggcagctg ggccgactta
    34741 cccaatgcac cgaaaagaag caaggcagca gtaataaccg cgaggggtga cccgactgac
    34801 catttatcat tgacctgcag catcagctcg tccaggtgga aagtcctgaa attcacgaac
    34861 agaataaaga tcccgatgat catggccgta tcccctatac gggtaactat gaaagccttc
    34921 cttgccgcgt acccattctc cggctccttg taccagaaac cgatcagcag gtaactgcac
    34981 agtccgacac cctcccatcc aaagtaaagc agcaggatat tatcagcgag gacaaggatc
    35041 agcatggaac ccacgaaaag gttcatatag gcaaagaacc ttgaaaatcc ttcatcaccc
    35101 gacatgaact caatggaata gacatgtatc aggaagccca cgaaagtgat cacgatgcag
    35161 aaaaccagcg aaagggaatc aagactgaat gacatgtctg ccctaagctc cccggcacta
    35221 atccattccc ataagctgac actataggaa cttacccccg gcaatgacct gaggaacaat
    35281 gcgccggcta tcagggcaag taccagtgaa ataccaaccg aacctgcacc gatatacccg
    35341 gcggttttcc tgtcgaactt tcccccggca acagccagga tcagaaaacc aagcaggggt
    35401 ataaggggta tgagggcaat acaagtttcc atcatcgctt tattgattgt tatccttcag
    35461 tttcccgaca gaatcggcat caagggtctt ataatgatga taaacctgca gcagcagtgc
    35521 aaggccaata gccacctcaa ctgcggcgac tgcaaggatg aaaataaaca tgacctgacc
    35581 gtcggcctgc ttccaatgcc gtcctgctat aatgaacacg aggccggcgc tgttgaacat
    35641 gatctctatc gacataagca tgaagatcag gttcctgcgt gtaagtaacc ctaccaggcc
    35701 gataaggaac aggagcccgg caaaaatcaa catcgtttca atcggaacag tcagcatctc
    35761 ctatttcttt aaaaacctgt gagtcacttt tttcttctgg ctgcccaggt gataagcacc
    35821 gatgacgccg gccatcagaa gaatacctgc tatctccacg gccaaaacgt atgtagaaaa
    35881 caaggatatc ccaacctgtt ttgtattaag agtttcatca acaggctccg gaatctgcag
    35941 cccgtttata atcaggatga aatcaaccag gaggagagcg gcaaggatag aaggtacaat
    36001 ccacactcga ggattgagcc atcttttctc tatctttttt tccgttccga tattaagcat
    36061 catggtcacg aaaatgaaca gcaccatgat ggcgcccgcg tatattatca cctccagggc
    36121 agctatgaac ggtgcatcga gaagataaaa gattaccgag actgccagga gcgatatgat
    36181 caggttcaga agggcatgaa tggcattcct tcccgagata gccattatcg ttgatatcag
    36241 ggcgattgcc gcggccaggt aaaagaaaaa gttcatagca tcacgatttt atggcataag
    36301 atcttttaca tcaacaggtt tatactccgt atccccttct ccttttcctt tgacacccat
    36361 ggccaccccc gcgtgtttgt aaaaattgta tccatgatat ttcccttcac cgcttatcag
    36421 caggtgttcc ttttcataaa ccaggttctg cctgatgtat tcacccatct caaaatcagg
    36481 aataagctgg atagcattag tggggcaggc ttcctcgcaa aacccgcaaa aaatgcatct
    36541 ggagaaattt atccggaaaa attcgggatg acgtctcccc ttctcacccg tcgcggcctg
    36601 caacgagata caatccaccg ggcaaaccgc cgcgcaaagg taacaggcca cgcagcgttc
    36661 gttcctatcc gggtcgcggg tcaatactat cctgccacgg tagcgcgggg caagataagg
    36721 cttcacctcg gggtattcca ccgtttcccg tttgtgaaag atgtgaagga atgtcagcca
    36781 tatacttcgg aggatactga tcatatcagg ttcattttat ctttttcatc ataaaaatat
    36841 aagtattgca cctgtaacga gcaggttgag caaagcaatc gggaacagga gcttccatcc
    36901 cagtttcata agctggtcgt atctcggcct cggcagggaa gcccttagaa gaataaaaac
    36961 acctatgaaa atgaacgtct tcagcaaaaa ccagaaaaca gggggcagga atgaagggcc
    37021 cagccatcct ccaaaaaaca tggtggctat tattgccgag atgagtgtta tcccaaggta
    37081 ctcgccaacg aaaaacatcc cgaatttcat acccgagtac tctgagtgat agcctgcaat
    37141 cagctctccc tcggcttcgg ggatatcgaa aggcagcctg tgtgtttcag ctatccctgc
    37201 tataaaaaag accacgaaac caagaaactg gggcaggatg aaccaaccgc ccctttgtgc
    37261 ctcgacgatt tcactgaagc tgaaggtgtc agccatcatc acgactccca tcagcgaaag
    37321 ccccatgaac acttcatagg tcagcatctg ggccgaggcg cgcattgctc ctatcaggga
    37381 atacttgttg ttcgatgccc agccgccaag aacgatgctg tagactccaa gcgatgagtt
    37441 tccaagaaaa aacagcactc cgatattcga gtcaaaaaca caaaactccg ggccgaacgg
    37501 gatcacggca aaggtcagga gtatagtgac catgaggatg ccgggggcaa ttgtaaagac
    37561 cactttgtca gagaacggcg gcacccagtc ttccttgaaa aaaagcttca gggtgtcggc
    37621 tataacctgg gcaaggccaa aagggccaac gcggtttggt ccgagcctgt cctgccagac
    37681 agaaagcagc cttctttcaa cccatatcag cccggccgct attgacaggg ctatgaaaat
    37741 tacccccaaa atgatccaca gataattcat tgtaacccgg ttaaattttc aatatttccc
    37801 cgctggcagg aagatcaacg aagttcatcc caggcaggtt tacagtcagg cctgccaccc
    37861 cgcttacaag gctgttttca attttcactt taacaaccag tcgttttcca ctggtttcaa
    37921 gtcgcaccga atccccgtca tcaacgttta acctttcagc atcccgcctg ttgacgagta
    37981 tggcgggatc atcaaccagt tgcctgaggg aagaggcagc cgaacttaat tcctcgcttc
    38041 cgaatacccg gtagagggga acgatgagcc atccgtctcc ttctgattca aaatacccgg
    38101 ttatcatttc aaagctgccg gaattcaccc gttctgcctg cctgatcagc cgtatcccgg
    38161 gatccccgcc tttcattgac ccattcgggt gatcaatgta attatagaag gcctgcaccg
    38221 aattccaccc gggtgtccag taaaagggca ccagtgaaga aggaggtgac tcccggcggc
    38281 cttccatgct gaatgccagc ggagaatcag gatcctgcct gacacgtttt tcgctgactg
    38341 ccctgtgtgc attcatcgcg gtcctcccgc tatagcggaa agtttgtcgc ggcaccttgg
    38401 cattcagcat cctgaaatca gcatcgggaa tatattcctt aatcgctgaa aacactggca
    38461 atgccgttac cattgaagcc acgatatcat cgaaatgccc cggggacacg ggtacattgt
    38521 cgcttcttat tcctgaaacc tggttcagcc attgccagct ttcctttatc ccttcacctg
    38581 cggcaagaac cttgtaatat ctctgggctc ttccctcgtt gttcacgata gttccctgcg
    38641 attcggcaaa agcagtggcg ggaaggagta tgtcggctgc ccgcgccgtg gggttgttaa
    38701 ggtaatccag gacaaccacc ctgccggatt tcccgaacat gtttgccacg gtatcctcgt
    38761 ctgcccttct gaaaagatca ttctccaaaa tgataagagt gtcggttccc tgaacggaaa
    38821 gggtgtcagc agcatcatcg agtgatttgc caggcatgag agccagtccc atgctgttgg
    38881 attccggaag cacggggatg aacatgactt ttttattcaa cgacagaagg gaagaagcga
    38941 tactcattcc cgcgttaaga acattcccgt cgagacatgt tatacctgca ataaccaacg
    39001 ggttttcagc tttcagcagg gtccgtgcta tttgttcagc caggttccgg atttcgtcat
    39061 cgttatcccc ggggacgatt gcctgtctct ttataacaga gctgatatta aagcccagct
    39121 ttgatatccc ggccggggtg cccctgaatg atctttcagc gacatcatca agggaatcct
    39181 tcacaggggt tgctatataa accgggcttt tcctatcctg cgcgagttcg cgaacaggga
    39241 ggtcgttcca gaacgggatc cctttagcca gggcttcagt gatctgaatg ttcctggctg
    39301 cctgacgtac tgccagagcc atcatgggtg cagtgtttgt cagatcctcg ccaaggataa
    39361 taacggcatc agctttctca acctgtttaa gagaaggtac cacgatgccg ctgttttgca
    39421 taaactgcag ggctgccctt gtaagcgact gttctttccc ggagatcccg tggaagaaat
    39481 tctcttcccc gacgagcctc aacaacgcgt aatttgattc cagcgaagcc cttggagatc
    39541 caattcccat gatcttcctg tcctttgaaa acaagggttc aagggcagta aaaacctggt
    39601 cagcgggcat ctctgcctgt tcaagatccc tgctgtcacg ggtacgcggg gataatatcc
    39661 tgtccttccc attcacgaac tcgtatccga acctgcccct gtcacatata aaataaccgt
    39721 ttaccgcgcc attgtacctg ttcattatcc ttctcagggt atcgtatcgt tcgcctgcaa
    39781 ttatattgca gccaacactg cagtgaacac acacggaagg cgcgtttgac aggtcccact
    39841 tcctggtgta atgttccctg aatgttttgt ctgtaaacac gccagtgggg caaacctcca
    39901 caagattccc gctaaattca ttttgcaggg tgccgtcctc gtggcggcca aaaaacacgt
    39961 tattatgagt tgcaaaaaca ttgaagtcgc ggccgccggc atagtccctg tagaacctga
    40021 cacacctgta acactggatg cagcggttca tctcgtgact gatgaaaggg cccaggtact
    40081 gatcattatg ggtacgtttt ctaaaatcaa accgccggta attatgcccg ctcatcactg
    40141 tcatatcctg cagatgacat tcacctcctt catcacaaac ggggcagtcg tgcgggtggt
    40201 ttgtcatcag gctctcgatc accgcggccc ggaatgcctt tgcatcaggg tcgttgattg
    40261 atacaataag tccatcaact acaggttcca tgcatgacat gacgatcctg cccctggtat
    40321 cattcgtgtc acgaaatttt ttcacggcac ataacctgca ggccccaaca gaaccaagtg
    40381 ccgggtgata acagaaatgt ggtatgtaca gtcccagcga aagagcggta tcaaggaggc
    40441 tcttcccttc gtgagcctcg tacggtttcc cgtcaatccg gatattaacc actttcattt
    40501 ccatgggcat tttttttcct cgatatgtcg tttgaagtca tcatgaaaaa acctcagtcc
    40561 gctctgcagt ggttcagcgg ccccgggagc gtgagcgcaa aacgtgtggc ccggtcccat
    40621 gtatgaagta tggaatttca gaatatccag gtattccatt tttcccttac catcttcaat
    40681 ggctgaaagc attttctcaa cccatggcag gccttccctg catggtgtgc accagccgca
    40741 gctctcgagg gcatagaatt tctcgacgtt ctggaggaag gcgaccgggc aggtcctgtc
    40801 atcaagcact atcattgttc cggttcccag cctgctcccc gccgcggcga cagttgaaaa
    40861 atccatcttc acatcgaggt gttcttccac caggaaatcc gttgaagctc cccctggcag
    40921 gacgcccctg aaagcgtaac catcgtgcat cccgccggca tgctcattga ttatttcgcc
    40981 gatggttacc cccagcggca gttcccacag gccgggcctc ttaaccctgc cgctcacccc
    41041 gtacagtttt gtgcctccag tgccggttag gctcaagccc ttgaaccagt cggctccttt
    41101 ttcaactata tgcgatatcc acgacagtgt ctccacgtta ttgaccactg tcggcttccc
    41161 gaacaaaccg cttgtcgcgg ggaaaggcgg ctttgtccgt ggtatccctc tcttgccttc
    41221 aagagcgttc aggagcgcgg tctcttcacc gcaaatatac ctgccggcac tcgtgtgcac
    41281 gtgtatgtcg aggttgaaac cggtgccaag gatgttcttc ccgaggaaac cttcgcgata
    41341 tgcctccgat attgcttttc tgagctcgcg ttcagctttt ttataggccc agcgcaggaa
    41401 gatgtaagcc gtgcccgcct gtatcgcgta agcagccgcg atcattccgg cgatcagctg
    41461 atgaggatta ccttcaagca gcaggcggtc cttgaaagtg ccgggttcca tctcgtcagc
    41521 attgcacacg aggtacttcg ggaaaatatt ctcgccctcc atcgggacaa ggctccattt
    41581 caccccggtc gggaaacccg cgcctcccct cccaagcagg tttgatgcct taaccaggga
    41641 agtcaccccg gcgggagtca tctctttcag cgctttaatg gttccattgt agccgccggc
    41701 acgataatat tccctgaagc cgggaggagc ttcccctttt ttaatatgtc cggtcagagg
    41761 cctttccatc aggattattt atatttctca agcagttcat caattttttc gaccgtcagg
    41821 ttattataat gatcatcgtt gatcatcatc gcgggggcgt tatcgcaatc gcccaggcag
    41881 gataccggca acagggtgaa acgcccgtcg gaagtcgttt caccaaagct gatctcgagt
    41941 ttttccgaga tatatttata cagcgttttg tatcccatga tcatgcagct tatgctgtcg
    42001 catataagga tcacgtttct gccaaccggc ctgcgataga tccgactgta aaaagttgcc
    42061 acgccatcaa cttcatcttc cgacatccgg agaatttcag cgatatcgcc tactgattca
    42121 tccgagaccc acccgcggtg cttctgaact attttcaagg catctatgca ggccgcggcg
    42181 ggataagggt aaagttctgc ctcctcaagt atttcctttt tttcttcctc agtcagcatc
    42241 tttaaaattt taagtttcac ctgtcgaggt cagcaagcac gaaatccatt gctccaagta
    42301 tcgacagcaa atcggggatc gtgtatcccc tgctgataaa gggcagcatc tgcatgtggg
    42361 ggaaggacgg ggttctgatc ctcgacctgt atgatgacgt gctgccatcg cttaccaggt
    42421 aataaccgtt gctccccttt gctgcctcaa tcggaaccat tgcctcgcct gcaggaatga
    42481 ccggtcccca cgaaacacca acgaaatgag tgatcagggt ttctatatcg gtcatcgttt
    42541 tctctttcct gggaggtatc acgagagggt gatccgactt gtaatggccg gccggcatgt
    42601 tgtcaataca ttgctgaata atgcgcaggc tttgccgcat ctcttccatc cttaccacgg
    42661 cacggtcgta gcagtcgccg ttcttgccga gcgggatatc aaaatcaaac ctgtcgatcc
    42721 cggaatatgg ccgtttcttg cggtaatccc aatcataccc cgcggccctc agacccgagc
    42781 cggtaagtcc ccattcaatg gcttcctcag cggtataaac acctatcccg actgtcctgg
    42841 ccttgaaaat ccggttttta atgatgacct tatcgaattc cctgagacgg gcgggaaggt
    42901 aattgacaaa atcctgcatt agtttctgcc agccatcggg caaatcatgc gccacacctc
    42961 ctatcctgaa ccaggatggg tgcatgcggg ctcctgttat tgcaataaca atgtcaaaaa
    43021 tacgctccct gtcattaaat gtatagaaga ccggtgaaag ctgccccagg tcctgggcaa
    43081 aggtacccag ccacaccagg tgactggcga tccgaaacag ttcactcatc attacccgta
    43141 ttaccatagc cctgtcgggg acctctatgc ctgccagttt ctcgacggac aacaggtagg
    43201 caaggttgtt catcacgcct ccaaggtaat cgatacggtc ggtgtaaggg atgaaagtgt
    43261 gccagctctg cctctcgccc atcttttcag ctccgcggtg atgaaagcct atctctggaa
    43321 cgatgttcac tatatcctcc ccattaagct gaacgacgag tctcaggaga ccatgcgtac
    43381 cggtatgttg gggaccgata ttcaggaaca tgaaatcgct gtcctcagcc gaacgggcaa
    43441 gtccatgatc ctcaggtttg aacctcaggt tttcatcttc cagatcacgt ttctgctctg
    43501 tgagtacaaa aggatcttgc tctgttgccc tggcatggtg atccttcctc agcgggtgtc
    43561 ctttccagct ttcgggcatc agtatccttg caaggcgggg atgcccgtca aaacgaatcc
    43621 cgaacatgtc gaacacctca cgttcatacc agttggccga ttcccagaca ccggtaaggg
    43681 tgggtatcgc aagatcattc tccttcaggg gaacctttag acggatatct tcatttctgt
    43741 ccagggaggt aaggtgataa acaaccgtga aatcgccgcc cggctgtacc ggattacttt
    43801 ccctggctct ttcatcgatg gctgtaagat cgtacaacat cttgaaaggt ccgggcactt
    43861 ctgtcttgag gtattttaaa accggcacga taaattccct ttctgtccaa agggttgtta
    43921 taccgtcctt tgtttcctgc ctgacaaagc cggcccggct gccaaacctt tcaatcaaaa
    43981 catctgatat gcctgaatta tcactgctca ttggctacac tatcctgaac acacgctaaa
    44041 cctcattcat tggtcttatg tttgtcattc ttgacctctc atcttttttc agatccctca
    44101 tgcaaggttt ttctgcctta taaaccccgt tcgggccaat aacccagctc aggggtcgtt
    44161 cttccctgcc tatcgaatcg cgcaacagca cgattccctc aagaagggca tcgggcctgg
    44221 gagggcagcc cgggacatac acatccaccg gcagaatttt atcaactccc tgcaccacgc
    44281 tgtaaatgtc gtacatacct cccgagttgg cacacgatcc catcgagatc acccagcggg
    44341 gttccatcat ctgctcgtgg agtctcttta ttatgggggc cattttcatg aacacggtcc
    44401 cggcaacaac catcaggtca gcttccctgg gagtgccgcg aatcacctcg ccgccaaagc
    44461 gagccaggtc atacctgctg gtgagcgagg tggccatctc gacaaaacag catgacaggc
    44521 cgaaaccgaa gggccagatg gagttttttc ttgcccaggc cagcaattca tcaagcctgg
    44581 caaaaacaaa cgagtcataa aggcttcttc ccgaaagttc tttttcaaaa cccgtattta
    44641 tcgcaccagt ctttttcatt tagtatcttt ttggggtcgg ggaatatttt taagaatctt
    44701 tttcccatcc gggccaaaat ccagtgcccc gatccttagt tcataaacaa gaagaatgat
    44761 aagtaaaacg atgaagatga agaccccgat gtaacccgcc agtccaagct cgcggaacga
    44821 aatggcccac agcattataa aggcggcgtc gaggtcaaag atgacgaaaa acattgctat
    44881 tatgtagaaa gcagaggaga accggagcct ggcatcaccc gtgggaggta tccctgattc
    44941 gtaaggttca cctgttttct tttcactgtg ccgctccccg aggacatgag aaagaccaat
    45001 catcactgcc gccagggcaa caacaatagc tgcaaaaacg ataaagggcc agaaagtgat
    45061 ttgttgctcc aaggatccag gtttataaat agcagacttt gagaacaata atactccgga
    45121 aaattgcccc gcgttgactc ctgccctgca aaccgatcac attaacaaaa atatatattt
    45181 attgtttcta tgcaacagtt tctttattta tatcagtctg acgctttttt aatgctccgg
    45241 caatccctga acggaattgc attaatatga acagccataa ttaaataata acagcatact
    45301 gttgataaga tgtcagtatc gctgttgata agatgtttgt ataatgaatt aacgggggtt
    45361 atagtgattg ttaacagctg caaatgcata ctccggtctt tccgccggcg gtacatagtc
    45421 aatcatttct ccgccagaca ggaagcagtt agcgccttca ggtgcctctt tcatttaaac
    45481 aattccatat gcgttattct aaaacccttt cactaaagaa aagacttcat tatttaacgt
    45541 tatgaatttt atattaacgc cttattccct tacctttaaa aaaaatctca attccgtcca
    45601 attgtttact aattatctgt attcggtcat atggcccctc tgatatctca cgatgctaaa
    45661 accatttctc tatcataatc ttatcgaagc cggctgtgac gaagccggcc gcggttgtct
    45721 tgcaggtccg gtgtttgccg ctgccgttat cctgcccagg aatttcaggc atgatctgct
    45781 gaatgattca aaaaagctta cctcacttca gcggttccgc ctgagggatg aaataatccg
    45841 ttctgcagtc gcctggaaag ttgcttcagt aagcaatgag gaaatagacg agatgaacat
    45901 actgagagca tcgatcaagg caatgcacct tgccgtcggg ggacttgtcc ggaagcctca
    45961 gttcctgctg atcgacggaa acaggttctt tccgtactcc ggcattgaac acaggaccat
    46021 aatcaggggc gacgccatgt tcttatcaat agctgccgca tcggtgctgg caaaaacatt
    46081 ccgcgacgag ttcatggaaa agatccataa cgaattcccc gattacggat gggacaggaa
    46141 caagggttat cccaccaggc agcacaggga agcaatactc aggctcggca tcacacgata
    46201 ccacaggagg tcattcaacc tgctgaatac ccagatgaaa ctggatttct gagcgggccg
    46261 gagaataaaa aactatattt gcatatgatt gtccagaaac aatacacctc aaatacaccg
    46321 catatgaaga aattactatc cctgatggcc atactggtca tcgttgcagg aataagggcc
    46381 agggccgatg aaggtatgtg gctcctgccg cttattgaga aactgaacat gggaaagatg
    46441 accgaactcg gacttaaact gtcagccgaa gatatttaca gcctgaacaa accaagtata
    46501 aaagacgcca tagtgatttt cggaggcggc tgcaccgggg agatcgtctc ttccaggggg
    46561 ctgctcctga caaatcatca ctgtggctac agtcagatcc aggcgcacag ctcacttgaa
    46621 aatgattatc tcagagatgg attctgggcc atgaacatgg aagaagaact gccaaatccg
    46681 aacctgtcgg tcacttttct gatcagtatc gaagatgtca ctgataaggt acttgcagat
    46741 gtcagaccgg aaatgaatga aagggaaagg aattcaaaaa tcagcattgc caggaaccgg
    46801 attgagagca aggctacgga atgcaatgat tacatggcaa ccgtatcttc tttctatgga
    46861 ggcaactatt tttaccttct ggtataccag cgatacactg atgtaagact ggtaggagcg
    46921 cctccttcat caatagggaa attcggcttc gacaccgaca actgggaatg gcccaggcat
    46981 accggtgatt tcagtgtttt cagggtttac accggccccg acggaaaacc cgcaccctac
    47041 tcgaaggata atatacccct caggccgtct tactggctgc ctgtctcaat aaaagaccta
    47101 aacgccggcg attttgccat gatcctcggg tatccgggaa gaacacaacg ctatttcaca
    47161 tcatttgaag tggatgaact gatgaagatc accaatccca acaggataag gatccgcggc
    47221 atcagacagg agatcctgat ggccgacatg cagtctgatc cgaagataaa tattcaatat
    47281 gcttcaaaat attcaggatc atcaaattac tggaaattct caattggtca gcaggccggc
    47341 ctcgagcgac ttaaagtcat ggaaaagaag gaacggatcg aaaaacagtt caacgactgg
    47401 atcgcagctg acggggaaag gaagctgaaa tacggggaaa gcctgaacat gataagatcg
    47461 gccattgaag aaagggcaga gtatctgaac gcacagcaat acctgaatga atgcttccgg
    47521 cagggatgtg aacttttaaa cctgtctacc ctggcttcaa tgcttacccc gtctctcaaa
    47581 tcgggcgaca aacagaaaac agaaggttat atcgcaagga taaaaagcta tgccgaatca
    47641 ttttaccgga attataaccc gccaaccgat gaaaaggcaa caaaggcaat gattgagctc
    47701 tacaggcagg atgttcccgt gaaattccat cctgatttct tcggcagcct gatagataaa
    47761 aaatataagg gcaatatcga cagattcaca gccgatgttt tctcaaaatc aatttttgca
    47821 aacgagtcaa gattcaatgc gttccttgcc aacccggtcc ttaaaaccct ggagaatgac
    47881 cctgtttata tcatgtcaaa ctctgtaagt gacatgagca cttctgtttc caggaacctc
    47941 agccgttacg atgaggatct tgcaaccggc agaagactct ggatcgctgc actgagagaa
    48001 atgatccctg aaaaaaccct ttaccccgac gccaattcca caatgcgcct gacctacggc
    48061 acaatcagtg actatgatcc taaagatgcc gttacatata aattttactc taccctgcag
    48121 ggtgttgttg aaaaatacaa gcccggagac tatgaattcg atgttcccca aaggctcctc
    48181 gatctaaatg cacggaaaga attcggcaga tatgcctcac caaaaggcta tatgccggta
    48241 tgcttcctga caacaaatga cattacgggc ggaaactcag gaagcccggt catgaacgcc
    48301 agaggtgagc tcatcggcct ggccttcgac ggtaactggg aggcaatgag cggcgacgtg
    48361 gcatatgaac cggatcttca aagaaccatt gccgtggata tcaggtatgt cctgtgggtg
    48421 atggatattt atgcaggagc gggccacctg gttgacgaaa tgaccctggt tcagtagaac
    48481 ctgaccgcca tgagaaaccg gtgaataaac aattccagga tatggatctg tttacggttt
    48541 ttgcaggttc tgacactgag gcctggagag aaaagatgct tcctctgagg ttcactggtg
    48601 atccgttttt acaagaactc caatactgaa gttcaggaaa tacttacccg ggcaatcaaa
    48661 tattcggtca ggcaaaatgt tccgcttttg agaatattga ctactgaata atgcgaatta
    48721 agagttgact tagaataagt tattaacata taaaatacag att
//
LOCUS       DAOOYG010000006        45296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_675_length_45296_cov_7.269291, whole genome shotgun sequence.
ACCESSION   DAOOYG010000006 DAOOYG010000000
VERSION     DAOOYG010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 45296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45296
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_675_length_45296_cov_7.269291"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /gene="ccsB"
                     /locus_tag="PK288_01230"
     CDS             <1..1317
                     /gene="ccsB"
                     /locus_tag="PK288_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HPM17567.1"
                     /translation="YKLSDLVNKVYSKAPGERSKLDKEIMKVDERVNIVYMIYTGGFL
                     KIFPKKDGQHDWDSPQQAVRHAAGKEDSLYLHSVMPMFAEALRSGNRTAASAISASIV
                     EYQQRFAGYDLPPVRRINAEILYYKLMIFERLFPFYATIGIIMLVSLLIMVIGGIRTD
                     TFILKALKWLLLAGFILHTFGIGLRWYVSGHSPMSNGYESMIFISWVTLFAGFIFSRK
                     SDFTLSATAVLASMTLMVAHLSFMDPEITNLVPVLKSYWLTLHVSVITGSYGFLGLGA
                     ILGIINLILVALSGERNRERISATVDELTVINYKTLTIGLYLLTIGTFLGAVWANESW
                     GRYWGWDPKETWSLITIVVYTLVTHSRMIPGLKDIYSFNLLSMIGFFSVLMTYFGVNY
                     YLSGLHSYAGGDPVPVPAFVYVSVVILALLSVTAYMRYRKWEKGNG"
     gene            complement(1703..1778)
                     /locus_tag="PK288_01235"
     tRNA            complement(1703..1778)
                     /locus_tag="PK288_01235"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1741..1743),aa:His,seq:gtg)
     gene            1919..3307
                     /locus_tag="PK288_01240"
     CDS             1919..3307
                     /locus_tag="PK288_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPM17568.1"
                     /translation="MSPERPFTVSIPKLRKIKLIDNISGLQLFQLMRFTVFLIISIVF
                     TKSHLTRAEIGSWEMFLFISGLISFFWVTGIIQSLLPLYHRNKTYRKIGENGKGKSPE
                     IFNAFILLCLFSLVFFGMGHSLKNHFSVFNISGNVPYLNLLLLYLLLSNPVCLIEYIY
                     LLNNRSYRIFQYGIYTFSLQLVFVISPILFRKDIIWAVYGLLAITAVRWVWLIILLRR
                     YTEMQISVKFIREHLYLGFPLIITSLLSGSAQYANGVIISAVYRDPGMFAWFRYGAKE
                     FPLVLMLANGLSNAMLPEFSTRSRMKESLTRIKYKSQRLMHLCFPATMLIMLLARWFY
                     PRMFNPEFQKSADVFLVFSLFVIPRLVFPQTVIVGRKKTHITLIAAVIELGITITLSL
                     LMIKWGYNIVGVALASFIGYTVGKIYLSGYLWVKMKIKPDDYIPLKAFLIYSILLVAV
                     FVLIDHRIIDIH"
     gene            3507..4733
                     /locus_tag="PK288_01245"
     CDS             3507..4733
                     /locus_tag="PK288_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17569.1"
                     /translation="MQIIQVTTKRDREEFLNLPRRLYKGDPNWVCQLDSELEALFDPE
                     RNYLFRQGEANRWILKDDSGRTIGRIAAFIDKVRSDAHFQPTGGIGFFEVINDKNAAF
                     MLFDAGREWLTSKGMEAMDGPINFGENDNNWGLLVDGFMQQGYGMPYNKEYYRSFFEE
                     YGFRNYFEQYSYHRDVRTSDNKIVEFPPRIMKIAEWLSKRPGYSFRHFEFARKQKFID
                     DLVEIYNATWKSFKEDFTPLDPELLDALFRKAKPVIDQDIIWFAYFNDKPIAFFILYP
                     DFNQILKSLRGKLHLLNKIRFLYFKLTHKMTRMRAVVGGVLPSYQNSGVESAIFYNLY
                     EVFKRKPWYKELELSWVGDFNPKMIAIYEALGARKAKTHITYRYLINKKLKFTRYKVE
                     MAGIPDSEPDQPGNKK"
     gene            4814..5077
                     /locus_tag="PK288_01250"
     CDS             4814..5077
                     /locus_tag="PK288_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HPM17570.1"
                     /translation="MKNGIHPSNYRLVVFQDMSNGYSFLTRSTAPSRETIKWEDGNEY
                     PLIKLEISNTSHPFYTGKMKLVDTAGRVDKFMNRYKDHVGRKK"
     gene            5264..7675
                     /gene="lon"
                     /locus_tag="PK288_01255"
     CDS             5264..7675
                     /gene="lon"
                     /locus_tag="PK288_01255"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPM17571.1"
                     /translation="MDGTGDIVPIIADGEDGDLEDVEVPETIPVLSLRNTVLFPGVVI
                     PISIGRPKSIQLIKDVYKSDRIMGTVAQRDPDTENPAFPDLHTVGTIGQIVKLLEMPD
                     GSTTAIIQGRKRMVLQELVADDPYFIARVKPIPETRPELPSKDFDAVVGSLRDLSLKI
                     IKINPNISPEASFAIKNIENSTYLINFICSNTDIKVQDKQRLLEINNLRDRGYQLLEY
                     LVQEIQLLELKNELQSKVKSDLDQQQREFLLHQQMKTIQNELGGNPLEKEIEEYRKKG
                     ESKDWPAEVRKTFEKELDKLNRLNPAAAEYSVQVNYIQTLLDLPWNLCTEDNLDLNNA
                     RHVLDKDHFGLEEVKERILEHLAVLKLKGDLKSPILCLHGPPGVGKTSLGKSVAKALG
                     RKYVRMSLGGLHDEAEIRGHRKTYIGAMPGRIVQNLKRAGSSNPVFILDEIDKVGQDF
                     RGDPSSALLEVLDPEQNSTFFDNFLEMEYDLSRVLFIATANNLATISPALRDRMELIE
                     ITGYLVEEKLEIAELHLLPKQIENHGVRPDQISISRKVMETIIEKYTRESGVRELDKK
                     LAKILRVTAKNIASGISYETELSEKKVIEILGPPKYVRDIYAGNEQAGVVTGLAWTAA
                     GGEILFVETSLSRGTGKLTLTGNLGEVMKESAIIALEYLKSNARLLALPDNFSEKWDI
                     HIHIPEGAIPKDGPSAGITMATSIASAFTQRKVKKFLAMTGEITLRGKVLPVGGIKEK
                     ILAAKRASIKEIIISEDNRKDVEDINEIYIRGLKFHYVDTIMEVLELSLLRQKVKNPK
                     KITFE"
     gene            7672..8154
                     /gene="coaD"
                     /locus_tag="PK288_01260"
     CDS             7672..8154
                     /gene="coaD"
                     /locus_tag="PK288_01260"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPM17572.1"
                     /translation="MKKIAVFPGSFDPFTIGHEGIVKRALNLFDEIIIAVGANALKKN
                     YYPVHVRKEMIGRVFQDEPRVKVDHYEGLTVDYCIRHNARYILRGLRTAADFEFERAI
                     AQVNKALAADVESVFFLTIPEHSPINSSIVRDIIRSGGDASRFVPAAIKLKDYKGEEE
                     "
     gene            complement(8170..8781)
                     /locus_tag="PK288_01265"
     CDS             complement(8170..8781)
                     /locus_tag="PK288_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPM17573.1"
                     /translation="MYKVHFEDRFLLISAEPDRLQKYGLFYKFGATKELYKLISDFQA
                     DDSIYSINIFGTNIRHIWRIFRIYFIEVGAAGGLVRHSSGRYLFIERKGRLDLPKGHI
                     ESDEDPETCALREVQEECGITGHKITRFLDSTYHTYTCRGISYLKNTRWYLMDYSGTM
                     DIKPQLEEGITKAEWLTKEEVSRLKAGAWHSLMDLINISILKD"
     gene            8788..9429
                     /gene="pyrE"
                     /locus_tag="PK288_01270"
     CDS             8788..9429
                     /gene="pyrE"
                     /locus_tag="PK288_01270"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPM17574.1"
                     /translation="MEDCATKIADYLLQIKAIKLQPSNPFTWASGWKSPIYCDNRKTL
                     SFPEVRSFISEKFAEYVRNLYPDAEIIAGVATGAIAHGVLTADKLNLPFIYVRSGAKE
                     HGLGNQIEGYFEPGRKVVVIEDLVSTGGSSLGAVKALREAGCDVLGMLAIFTYEFERA
                     ADGFAAQGCKLDTLGNYSTLIETALSSGYIGESELETLKRWRTDPSGWGQKSD"
     gene            9430..9855
                     /locus_tag="PK288_01275"
     CDS             9430..9855
                     /locus_tag="PK288_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17575.1"
                     /translation="MGQSAEYTSREGVLTCTPREIFEFAADMGNMINLLPPDTAKIIE
                     STGESCALSFDPLGSMKIQIIRKTPYSEVVYRAELLDIQNLLLNLEITGGDSEKAKVV
                     IRVNAEMNPFMKMMAEKHIARFLDILILEMEKFRDWKKS"
     gene            complement(9850..10593)
                     /locus_tag="PK288_01280"
     CDS             complement(9850..10593)
                     /locus_tag="PK288_01280"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPM17576.1"
                     /translation="MIIGSKIIFIRNISSTNTFASGLLKKDPVPAEGTVIYTDYQTAG
                     RGQRENKWESEAGKNILCSIILFPGMVAPEDQYVISMTISLGIYDFISEKVHGCKIKW
                     PNDIYVGDSKIAGVLIENSILDGKIGSSIAGIGLNLNQTRFISGAPNPVSLKMLTGEE
                     YDPEICLKQLIKNLDKSYKQLLAADHENIRSRFSSLLYRLNEWHSYRAGDMIFEGKIA
                     AVTGRGNLTVVDRAGKSLEFGTKEIEYIL"
     gene            10733..11113
                     /gene="rsfS"
                     /locus_tag="PK288_01285"
     CDS             10733..11113
                     /gene="rsfS"
                     /locus_tag="PK288_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPM17577.1"
                     /translation="MKKRRDETELLLENIIRGIVEKKGKDILKIDLRGLENRITDFFV
                     ICHGSSVTQVDSITSSIEDTVRTGTGEKPGHIEGLDNCFWVLLDYGNVVVHVFLEEYR
                     SFYSLESLWADASLEAIEDNQINY"
     gene            11117..13231
                     /gene="ftsH"
                     /locus_tag="PK288_01290"
     CDS             11117..13231
                     /gene="ftsH"
                     /locus_tag="PK288_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPM17578.1"
                     /translation="MAEKNNKNNNPGLGNSSDKNAKPRFNSNWIFAILAFSIIAFQFL
                     YGGRSVPKAQTSDLKKMVENRDIERIVVVNKEVAEIYLKKEAIESDRYPNLNKNGSRS
                     IGLSTPKPNFTYNISDISIFYKFLEDEQKKAGYEEKDVIYPESETRRNYIGDILGWTL
                     PFILLIFLWIYMMRRMSGGGAGGAGGIFSVGKSRAQIFDKDTNVKINFNDVAGLEEAK
                     TEVMEIVDFLKNPKKYTQLGGKIPKGALLIGPPGTGKTLLAKAVAGEANVPFFSISGS
                     DFVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAVGRARGRNVGFSSNDERENTLN
                     QLLTEMDGFGTNMGVIILAATNRADILDRALLRAGRFDRQIHVELPDLNEREAIFKVH
                     LRPIKMEKNFDISFLAKQTPGFSGADIANVCNEAALIAARKNKNIVEKQDFLDAIDRI
                     VGGLERKNKIISLEEKKTIAYHEAGHATVSWLLEHASPLLKVTIIPRGRALGAAWYLP
                     EERQITTREQLLDEMAYALGGRASEELVFGKISTGALSDLEKITKQAYAMVSFFGMSE
                     KVGNISFYDSSGQSDFGFTKPYSEKTAELIDREVKLIIEESYQRAKDVLSANMKGLTE
                     LAELLLEKEVIFSEDLERIFGKRKGDKLKAESEAQAKKETEAEPDLVEINKESGENKT
                     GRKKGKTASQKNGDIPDPGKSG"
     gene            13272..14114
                     /locus_tag="PK288_01295"
     CDS             13272..14114
                     /locus_tag="PK288_01295"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPM17579.1"
                     /translation="MSNFFRRTLTAIWIVIFVLGGFWLHPVSFFLTGLIIVACTQYEY
                     YSMIKYTGIRPQVAGGITAGLSAFTVSTMVAAGLLQSRWYLLLIPLILILMIAELFRK
                     TEKPFDSLAHTVFGIIYVALPFSLFPSAAFARTGLGSLIPHQGIIFSPGFIVGFFLVL
                     WANDTGAYITGVSFGRHRLLERISPKKTWEGYLGGLALAIFAAWLLGDWFGVMKKSHW
                     MGIAVIVSVAGTLGDLFESLLKRSVGVKDSGSVMPGHGGFLDRFDSALFAFPLGYLFL
                     SLFG"
     gene            14154..14810
                     /locus_tag="PK288_01300"
     CDS             14154..14810
                     /locus_tag="PK288_01300"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPM17580.1"
                     /translation="MGIHKEGYKILIYGFIMLVFVNVLAGLIWPDMVLLRYTVLIIAF
                     LMYIFLLFFFRLPVRYLEPDPGLIYAPADGKVVVIEETEEKEYFRDMRLQISIFMSPF
                     NMHSNRYPVSGKIKYVMYHPGNYMVAWHPKSSEMNERSSIVIETEKGAEIMIRQIAGA
                     VARRIVTYAKTGAHVTQGDELGFIKFGSRVDVFLPLGTEVEIPILQQVKANKSIIAKI
                     "
     gene            14824..16047
                     /locus_tag="PK288_01305"
     CDS             14824..16047
                     /locus_tag="PK288_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPM17581.1"
                     /translation="MKQKDDKIIDLTEHVKWIGILDYDIRTFDIVMNTEFGTTYNSFF
                     INTERKTLVDVAKEKFSEVWFDKLRALTDPSDISYIVINHTEPDHSGSLGMLLDAAPS
                     ATVVGSGSGIRYLADMIAKPFNSIVVKDGDTLDLGSGKLKFISAPNLHWPDSMFTWFE
                     EDRILFTCDVFGAHYCDSRMFSDIESQDYMKAYKYYFDVIMKPYSRFMIKAIEKIRDL
                     DINLICPGHGPVHSENLRDMVMLSENYAIEYLKQVESKDEKKILVAYVSAYGYTKQAA
                     DLIASGMRETGDFSIDIADIETMPAEELESKLVSSDAILIGSPTINQNTLLPVYRLFS
                     LINPIRDKGKMAGVFGSYGWSGEAPKIISETLRNLKLRVFDETAGFKFAPGNGKKETL
                     TEFGRQFAREFLKYC"
     gene            complement(16184..16609)
                     /locus_tag="PK288_01310"
     CDS             complement(16184..16609)
                     /locus_tag="PK288_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPM17582.1"
                     /translation="MLPIITRRSYRPFFLNGLFDDDFLPATTSLNRSVPAVNVRENEK
                     SFALDVAIPGINKKDIKIDLNDDLLTISHEVKNESEKNGNGYKRREFSYSSFCRSFQV
                     PDSVNKDKIEASYKDGILNITLPKYDEEKHKLTRQVKVS"
     gene            16851..18068
                     /locus_tag="PK288_01315"
     CDS             16851..18068
                     /locus_tag="PK288_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPM17583.1"
                     /translation="MKATLRNFLIFFGVLILLAAVWYFRNIVVYILVSGVLSIMGRPL
                     VDLFCRIRIWKWKFPRVLAALFALIIIWGSILLFLYIFIPLVTKQINHFTGIDSEKIV
                     RLIEGPLEKIEKLFVSLNPSISDTLSLKAYIASKVSEVLSIDMIQDFIGSIIGVLGNV
                     VVAIFSISFITFFFLKDQHLFFESILMWIPDRYVDNVTRALNSIKKLLTRYFIGIVIQ
                     STCIMILIDIGMTIIGIEFHQALVMGLIIGILNVIPYVGPWLGLFIAIIMGVASHINQ
                     DFNTVVIPLVYYMIIVEAVTHTIDNVVFQPVIFSSSVKAHPLEIFVVVLAAGFAAGVP
                     GMILGIPTYTVLRVFAREFFYGFKAVQKITSSLSDENSPEKSRISNVLIEESKRLTTR
                     KRSGKKSPGKAEE"
     gene            18079..18306
                     /locus_tag="PK288_01320"
     CDS             18079..18306
                     /locus_tag="PK288_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HPM17584.1"
                     /translation="MGSSPVLIKRVFRFYYDGFRSMSWWGKRVWIIILIKLFIIFIIL
                     KLFFFRNFLNEKFDNEKQKSEFVLDQLINSK"
     gene            18311..19867
                     /locus_tag="PK288_01325"
     CDS             18311..19867
                     /locus_tag="PK288_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPM17585.1"
                     /translation="MIDVPDLTLVDWSRAQFALTAIYHWLFVPLTLGLSFIVAIMETI
                     YVKTGLEEWKRMTRFWMTLFGINFAIGVATGIILEFEFGTNWSYYSWFVGDIFGAPLA
                     IEGILAFFLESTFVAVMFFGWNKVNKKFHLVSTWLVAVGASLSALWILVANAWMQNPV
                     GMVFNPETARNEMVDFWAVLFNEVAVSKFLHTISSGFLLGSMFVAGISAWYLLQKREH
                     HLATRSLLVAGVFGLLSSLLVVYTGDSSARTLARAQPVKFAAFEALYDGRSDAGLVAA
                     GILKDSDRKIGKKVVKDFSFRIEIPGFLSFMTGGNTRAFVPGINDLILGNPEKGLLPV
                     AEKMRRGKVAQDLLRDYKNARAGNDSVKMSTIRKVFADKQFIEEHFRYFGYASVENPE
                     DAIPNVPLAFYSFHLMVTLGFYFILLFCLVLFLLFRGTIAKQRWFLWILLFSILLPYV
                     AGEMGWVLAEMGRQPWIIQDLMPVSTAVSRIGSGSVITTFVLFAVLFTVLLIAEVSIM
                     IKQIKTGPNH"
     gene            19880..21010
                     /locus_tag="PK288_01330"
     CDS             19880..21010
                     /locus_tag="PK288_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPM17586.1"
                     /translation="MEPLISHLFLQHYWWIIISILAGLLVFLLFVQGGQSMIFSLPED
                     PLQTTILVNALGRKWEFTFTTLVTFGGAFFAAFPLFYSASFGGAYWVWMAILFSFIIQ
                     AVAYEFRSKKGNFLGQRTYDIFLLINGSLGPFLLGVAVATFFTGARFSLNELNRVSWA
                     TGWHGLEALVNGRNVALGLAVLFLARTNGLLYIINSVNDDGLYENAGRKIILNALTFL
                     VFFLFFLVTLLISPGFAYDPHTGNVFIEKYKYLHNLLRMPAVLIIFVAGVVGVLYGIV
                     ITVFRGSRKGIWFTGTGTILAVISLFLLAGFSNTAFYPSTFDLNSSLSIRKASSSYFT
                     LKTMMFVSFIIPFVAAYIWYAWKAINRRRISVEEIQEEGHVY"
     gene            21092..21217
                     /locus_tag="PK288_01335"
     CDS             21092..21217
                     /locus_tag="PK288_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17587.1"
                     /translation="MYFTGLAHYLIWPAFILVSWFIIKGALAVYERKFPGDKEES"
     gene            complement(21318..22058)
                     /gene="pssA"
                     /locus_tag="PK288_01340"
     CDS             complement(21318..22058)
                     /gene="pssA"
                     /locus_tag="PK288_01340"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPM17588.1"
                     /translation="MTLTKHVPNLITCLNLAMGFIAIIFAAGNDLIVSSWLIAAAMVF
                     DFLDGFSARLLKAYSDIGKELDSLADIVSFGIAPAMIVFQLISYGSTAGADSCSYFCN
                     PGTLKYITVLMPVCAALRLAKFNTDTTQTRSFKGLPTPANALAVISVVIAAHYSDAAV
                     FELFLKSPVAVTVYMIVLSLMMVSGIPLISLKMSNLGIKGNEGQYILFGLVIVSLILL
                     GIASAPLFIPFYIIASLISPMFAGKTEG"
     gene            22226..22663
                     /locus_tag="PK288_01345"
     CDS             22226..22663
                     /locus_tag="PK288_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17589.1"
                     /translation="MINFKNVKIAVDFDGTIVDHEYPDIGKEKLFAFQTLKELNKLGA
                     RLILWTFRTGDELEQAVEFCRRNGVEFYAVNKNYPEEVYDESISRKIDADIFIDDKNV
                     GGFPGWSEIWQILVPFELHEKEMERQIAAARKKSIRRLFGRKA"
     gene            22828..23792
                     /locus_tag="PK288_01350"
                     /pseudo
     CDS             22828..23792
                     /locus_tag="PK288_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
     gene            complement(23782..24735)
                     /locus_tag="PK288_01355"
     CDS             complement(23782..24735)
                     /locus_tag="PK288_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPM17590.1"
                     /translation="MKKELTANNWFTIVNPNAGNGKGRKDWDRINDLLAKNAIPVTVR
                     FTEKKGHATEYTREAIGKGFRKFISVGGDGTLNEIVNGAFTQEHCTPSELLLGMIPVG
                     TGNDWGRMFSIPLVYEGAVKVIMEGRQMPHDIGVIDYFNGENRERRYFINIAGLGFES
                     LVVIKTNRQKEKGKSSKAIYFFNLLASLLSWRHRNADIIIDGIKNSCKIFSINIGNGR
                     YCGGGMRQTPDALPDDGLLDITVIREMSRFEVIKSLKLLYDGTILSHPKVDGYRSTNL
                     SVQSDSLLYIEADGESLGHTPAEFRILPGAINIVYGSRIIR"
     gene            24998..25777
                     /locus_tag="PK288_01360"
     CDS             24998..25777
                     /locus_tag="PK288_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM17591.1"
                     /translation="MGRIIAIANQKGGVGKTTTAINLAASLAALEKKVLIVDADPQAN
                     ATSGIGIDTRQIKCTVYDCLIDGRDPSEALLGCGIDNLSVLPSNIDLVGAEIEMLERP
                     EREKILKNILGRISGNFDYIIIDCSPSLGLLTVNALTAANSVIIPVQCEYFALEGLGK
                     LLNTIKIIQNNLNPDLEIEGFLLTMYDSRLRLSNQVAEEVNKHFQQMVFQTIIQRNVK
                     LSEAPSFGQPAILYDADSRGTVNYLNLARELIEKQEVRVNA"
     gene            25813..26694
                     /locus_tag="PK288_01365"
     CDS             25813..26694
                     /locus_tag="PK288_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPM17592.1"
                     /translation="MTKKNALGRGLGALIDGVEKEVLERKVEANLNIPVDLIDGNPFQ
                     PRAHFDEQALGELTASIKKLGLVQPLTVRESGGGRYQLIAGERRLRAARLAGLTHVPA
                     YIRTADDQAMLELALVENIQREDLDAIEVAISFQRLIEECRLTQEQLSDRVGKQRSTI
                     ANYLRLLRLPAEIQLGIRNKHLMMGHARTLVNIENPKIQMEVYYHVVNDDLSVRQTEE
                     LVRQLQSEKIRDPLKDERRKKLNEEFKQLSEHLNRIFSTKVNFKINESGKGRIVIPFD
                     NPEEMERILGIFDRLNY"
     gene            26744..27391
                     /locus_tag="PK288_01370"
     CDS             26744..27391
                     /locus_tag="PK288_01370"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPM17593.1"
                     /translation="MNNLRATLKISLIFIVLQSCLYCNLPAQDTVNVIPAITRKRQFN
                     PVPLKATMLAVAFPGLGQVYNRKYWKIPLVYAGFGALAYSISFYTTHYNDMMKAYQDF
                     IDKVPETASYTRFVRIDPEEYDPVLHPETYKPSTASWVEDGLLSAVDAYKKYRDLSYI
                     GVAAWYLLSILDANVDASLFNFDISRNLDLALYPVMMPAAGTSVAGINLNLSYKF"
     gene            27502..29049
                     /locus_tag="PK288_01375"
     CDS             27502..29049
                     /locus_tag="PK288_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPM17594.1"
                     /translation="MIKLLKTLFLSLAISTYAFSAYSVNDTIILRSEIYAEKIATDLD
                     SLVNSWYVKMALKELEPDFQADSLGQEFPDSVYIERLNKINSVIRIPYNSIIRNHIHV
                     YTIRQRKNFSAVLGLLDYYFPMFEDIFDSMGLPTELKYVSVIESALNPNAVNRHTGAT
                     GLWQFMFSTGRLYGLTINSVVDERRDPVRATYAAARYLKDLYGIYNDWLLVIAAYNCG
                     PGNVNKAIRRSGNKKDYWEIYYRLPRETRGFIPQYVAALYAVNFYREHKIRPLPLNIP
                     VSTDTILVRKDIHLKQISEVMGIPLDELRALNPQYRTGLIPGSTKPFSLTLPMSHLGD
                     FIDLNDTIRKHKPEVYLTKVNRTEDPSRSSYVPADVKGKTKLIYTVKEGDNLGFIAEW
                     YKVPLSDLRYWNNIYRNTIHIGQKIAVYVDPSKAEYFSRVNSLSFAEKQQMTGKPAPA
                     SPVATPVLQGSDDDYIIYTVRQGDTIWDIIKRYDNVSPTDVLSLNNISDPGKIQVGQV
                     LRIKKKS"
     gene            30526..32046
                     /locus_tag="PK288_01380"
     CDS             30526..32046
                     /locus_tag="PK288_01380"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPM17595.1"
                     /translation="MFRTVIASVLTYNPEKPFVFSGPGFWVFFLVILGGYGLVYRRMN
                     TRNLYLFLISLYFYFKTGGLFLILLLLVILIDYSCGILIFNSRTRSKRKFFLLVSILS
                     NIGILAYFKYCGFLIHSFNDIFGTAFQVKDFLADFSNRLMGTRFDVGNIILPVGISFF
                     TFQSLSYTIDVYRGKLEPVRSIIDFGFYVSFFPQLVAGPIVRASEFIPQLRTEFHLDK
                     REFSHALFLVIKGLVKKIIISDFIAVNFVDRVFDIPTAYSGFENLLAIYGYGLQIYCD
                     FSGYTDIAIGLALILGFRLPVNFNSPYKAHGIANFWKRWHISLSRWLKDYLYIPLGGN
                     RKGKVRTNFNLMLTMLLGGLWHGADIRFIIWGGLHGLGLIVDKSLAAVFGSRTGSGRI
                     VKAFSIFLTFQFVSFCWIFFRATDMSHAGIMLNQIFHNFMPSSFGNIFEVYGTVFSLI
                     AVGYLIHFLPETLKESYRGIFIRIPVAVQMVMILAAGILLYHMRAYDVLPFIYFRF"
     gene            complement(32141..33364)
                     /gene="hydF"
                     /locus_tag="PK288_01385"
     CDS             complement(32141..33364)
                     /gene="hydF"
                     /locus_tag="PK288_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPM17596.1"
                     /translation="MAKGRDTKPHIGIYGRRNHGKSSLINKLAGQDIAIVSEQAGTTT
                     DPVRKSFEITGFGPVILVDTAGIDDSGELGRKRVERTIRTLDLIDLALLVVAENQWGK
                     PEDYLVEKFRSQDVPYIIVQNKSDLMKPTGEFREEVRRITGMELIEFSAVDNYNTEEL
                     ISLIRRSIPEHSYKIPGLLGDLVNPGDIVILITPIDVSAPAGRLILPQVQAIRDILDN
                     DAVAVVLKENEAGTFLEKSGLRPALAITDSQVFARADALIPHDIPLTSFSIMLARFKG
                     DFSTYLKGTPKISTLRDGDRVLLLESCTHHVACDDIGRTKIPGWISKFTGKHIEYEIV
                     AGLDVLPRPVSDYALIIQCGGCMITRRQLHNRLQPAIKAEVPVTNYGMAIAYVQGIYE
                     RAIAPFTRALTLNPD"
     gene            complement(33579..34757)
                     /locus_tag="PK288_01390"
     CDS             complement(33579..34757)
                     /locus_tag="PK288_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM17597.1"
                     /translation="MSKILVIDDEKAIRNTLKEVLEYENHEVDLAEDGPSGLEFFNSN
                     SYDIVLCDIKMAKMDGIEVLQKISENSSDVPVIMISGHGNIDTAVEAIKKGAYDFLEK
                     PLDLNRLLITIRNATEKTTLISQTQVLKSKVSKMFEIVGESPAILKVKEMIERVAPTE
                     ARVLITGANGTGKELVAHQIHEKSNRAKGPFVEVNCAAIPSELIESELFGHEKGSFTS
                     AIKQRIGKFEQANGGTLFLDEIGDMSLPAQSKVLRALQENKISRVGSDKDVHVNVRVI
                     AATNKNIRKEIADNTFREDLYHRLSVILIHVPTLNERTEDIPLLAEHFNSMICGEYGM
                     SRKIIRKDAISELQKIQWTGNIREFRNVLERLIILCRDEITGNDVIAYARPVSGMNIL
                     "
     gene            complement(34870..36231)
                     /locus_tag="PK288_01395"
     CDS             complement(34870..36231)
                     /locus_tag="PK288_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM17598.1"
                     /translation="MMNKVSVIIKREYLTRVRKKSFIIMTILAPLLMALLIVVPTLLM
                     MNQEQDYKKIAVIEDGTDLFRGKIGNTKNLEFVYLEGIRISDLKNSFEQAGYYGILYI
                     SPEIITTPNAIQLLSKKQPPIGMLEHIEKSLEKEIEKQKLKAYDIENLDEIMKNVETR
                     VSIQTIKIDDSGNVKETSTGVAMALAYIGGFLMYMLVFMFGAQVMRGVIEEKTSRVVE
                     VIVSSVKPVQLMMGKIIGIALVGLTQFLIWVFLTIAIAGSLKSTVLQKSDLQELSRDA
                     ARSLMAEETRVQTATSIPETDTRISEFGKLFETAMNQPWGLIIISFIFYFITGYLLYA
                     SIFAAIGSAVDNETETQQFMLPVTIPIILGLMVAMGTMQNPESQLSLWCSIIPLTSPI
                     VMIARIPFGVPYWQLALSMGLMVVTFAAFVWMAAKIYRTGILMYGKKTSWKEMWKWLR
                     YSG"
     gene            complement(36256..37164)
                     /locus_tag="PK288_01400"
     CDS             complement(36256..37164)
                     /locus_tag="PK288_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM17599.1"
                     /translation="MALFEAKNVTKQYGTQLALDNVSISVPEKCIFGLLGPNGAGKTT
                     LIRIINQITGPDSGELFLEGKKLKPDDVLRIGYLPEERGLYKKMKVGEQALYFAQLKG
                     LTKAEAMKRLRYWFNKLDIAEWWGKKVEELSKGMQQKVQFITTVLHEPKLIIFDEPFT
                     GFDPVNTNIIKNEILFLREMGSTIIFSTHNMESVEELCDNITLINKAKTILEGNVDDI
                     RRQWAANEYEIVFEGNAVLPEQEKINILKRHDENGRTRIRFRIRDGIDTNDVLGNALV
                     SGRIISFNPALPSMNEIFIRVVESRN"
     gene            complement(37239..38390)
                     /gene="dnaJ"
                     /locus_tag="PK288_01405"
     CDS             complement(37239..38390)
                     /gene="dnaJ"
                     /locus_tag="PK288_01405"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPM17600.1"
                     /translation="MDKRDYYEVLGVSREATKEEIKKAYRKQALKYHPDKNPGDKKAE
                     EHFKEAASAYEVLSNDEKRARYDRFGHEGVSGASGNGFGGAGMTMEDIFASFGDIFGD
                     AFGGFGGFGSSRRSSRRVNKGSNLRVKVKLTLGEIANGCEKKIKVAKYVVCSTCDGTG
                     AADQNSITSCTTCRGSGHVTRITNTMLGQMQTTSVCPSCGGNGKTITKKCPVCYGEGI
                     VQKDDIIKINIPAGVSKGMQMTVSGKGNAARRGGINGDLLVVIDEEDHPELIREGKDL
                     IYNLFISIPDAALGTHVEIPTVENIVKIKIEPGTQPGKILRLRGKGLPEVNGYGRGDL
                     LVNVNVWIPKNLTKEEARTLEKLRDSASFIPRPDKEDKGFFERMRGYFE"
     gene            complement(38442..39086)
                     /locus_tag="PK288_01410"
     CDS             complement(38442..39086)
                     /locus_tag="PK288_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPM17601.1"
                     /translation="MVRKKMSETVHGKETGQDKYTDSKDNKEIPIVNEQEEDKTLIDG
                     AGMGQNQFAGETLQDTPDKVVAEGRSFEEKLAEMQDRYIRIAAEFDNYRKRTLREKME
                     ITKYAGEDLLVKLLPVMDDFERALKHMEVSPDCQSLKDGLDLIYSKFSDFLKQQGIRE
                     IEALNKDFNFDLHEAVAKAPAPGEDSRGKVVEVILKGYFLHDKVVRHTKVVVGE"
     gene            complement(39288..40592)
                     /gene="murA"
                     /locus_tag="PK288_01415"
     CDS             complement(39288..40592)
                     /gene="murA"
                     /locus_tag="PK288_01415"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPM17602.1"
                     /translation="MGSFIVQGGKTLSGDIHPQGAKNEALQVICAVLLTSERITIRNI
                     PDINDVNKLIELLMALGVRVTRKSRGTASFEAVSINLDYLQSQEFRNQSASIRGSIMI
                     VGPLLARFGKAYIPKPGGDKIGRRRVDTHFTGFQKLGAKFEFDTADPFFSVEASGLKG
                     SYIHLDEASVTGTANIIMASVLAKGRTTLYNAACEPYIQQLCRMLVSMGARISGIGSN
                     LLYIDGVRQLGGCSHKILPDMIEIGSFIGLAAMTGSELTIKNVSYDNLGIIPDSFRRL
                     GIRIERISDDIFIPYQEHYEIETFIDGSIMTIADAIWPGLTPDLLSIFLVTATQAKGA
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLDKMMKLRGTTMSSPDIRAGV
                     ALLIAAMSAEGRSIIHNIEQIDRGYQNIDDRLNAIGADIKRI"
     gene            complement(40592..41239)
                     /locus_tag="PK288_01420"
     CDS             complement(40592..41239)
                     /locus_tag="PK288_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HPM17603.1"
                     /translation="MNYDYNTQRKRMALPEYGRNVQKMIDHIKTISDREERNRAAKTI
                     IHIMGNLNPQIRDVTDFKHKLWDHLALIADFELDIDSPYPPPEKFKFEEKPKQIPYKQ
                     GDIRFLHYGRIIELMIDAAAEMEEGEEKEYITNLILNQMKKSYVTWNRAQVSDEVIIS
                     DMKFLSGGKLKLKEGIKLLEVKELVPSAKKKPQQGKPHGKQQNKQFPKKKGHNRH"
     gene            complement(41307..42017)
                     /locus_tag="PK288_01425"
     CDS             complement(41307..42017)
                     /locus_tag="PK288_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPM17604.1"
                     /translation="MDNSITLLSVTAVSIGFIHTVLGPDHYLPFIVLSEAKKWTMKKT
                     MWITFLCGLGHVLSSVILGLTGIIVGISVNRLVEVESFRGNIAGWLFIAFGLIYMLIS
                     IRNLYRKRQHTHSHFHPGTGEHIHEHNHFGDHAHVHENDVVRTTPWILFLIFVFGPCE
                     PLIPILMYPAAQNNIAGSVFVSVLFSLVTIATMMGVVLAFKLGFSRINLKPVEKYSNL
                     IAGGMIFLSGIAIQFIGL"
     gene            complement(42124..43260)
                     /locus_tag="PK288_01430"
     CDS             complement(42124..43260)
                     /locus_tag="PK288_01430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPM17605.1"
                     /translation="MNHKILIPILAVLAVGMSPAQNPADSLTDRLSLLFIGDIMGHDA
                     QIRSAKDPETGSFNYDDVFSFIREEISEADYAIANLEVTLAGSPYKGYPAFSSPAELA
                     AACIKAGIDCFVTANNHSADRGKKGILETLQKLDSLGIPHTGTFASKNERDSLYPLML
                     NIKNNTIALLNYTYGTNGINVPDPVIVNMLNRDLVAEDLEKAVRKNPDLIILFLHWGT
                     EYDTIPSKMQDEMARFFFDRGADLIIGSHPHVLQKMIWTKEDPYGKGRIAVYSLGNFV
                     SNQQRPKTDGGAMVRIEIEKRNDSLKIASAGYYLTWVYTPFENGRKRFYILPCSKYER
                     NSEFFASPSDHIKMMDYVTNSRRFLEVQNINFREYIFTGNEWNR"
     gene            43355..>45296
                     /locus_tag="PK288_01435"
     CDS             43355..>45296
                     /locus_tag="PK288_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HPM17606.1"
                     /translation="MPKAGNNIDINKISAAGLLVTLGIVFGNIGTSPLYTMSAVLNGG
                     KGNFGDLLVYGSLSCIFWTLTLTTTIKHIAITLRADNNGEGGIFALFALMKNKSVWVA
                     VLTMIGGGALLADAIITPSITVTSAIEGLELFNPKIPVVPIVLVIIAALFFIQQFGTH
                     FIGSYFGPVILVWFLMLGLLGLSQIISNPLILGSLNPVYAYRFLSEFPGGFVLLGAVF
                     LCTTGVETIYVDLGHCGRNNIRVSWIFVKISLMLNYMGQGAWLLQNYKGGEDLNPFFE
                     IMPEWLLLPGIVIATTAAIIASQSIISGSFTLVSEAALLNFWPRLRVLHPTLVRGQVY
                     MPFVNMILWIACTIVVLIFKKSGNMDAAYVLAITITEIATTLLLAVYLFQKGVNHRLI
                     SFMVMVYLTVEGSFLIANLFKFSKGGWFTLILGLLFILIMLGWYFGRKIKNRYISFAN
                     LNKYTDLFRDLSQDESVPKTATNLVYIIRANRKDQVESKVVYSIFSKFPKRADKYWLL
                     HIDRVNEPNQFEYQVTQIIPGILIRVDFHIGFKVEPSINLYFREVLEDLSATGEIKLE
                     SGYESLRKYDIPPDFKFILIERIMPRDYKLSNWENFILFLNNIAGKMSISDIKALQLD
                     ATNTVIEHVPIIIDQPSHRRIQR"
BASE COUNT        12160 a         9804 c        10405 g        12927 t
ORIGIN      
        1 tacaagcttt ccgatctggt taacaaggtc tattcaaagg ctcccggaga gagaagcaag
       61 cttgataagg agataatgaa ggtggatgag cgggtaaaca ttgtatatat gatatataca
      121 ggcggattcc tgaagatatt tccgaaaaag gacggacagc atgactggga ctcaccccaa
      181 caggcagtca gacatgctgc cggaaaagaa gactctcttt atcttcatag cgtaatgcca
      241 atgtttgccg aagctttgcg ttccggcaac agaacggccg cttcagccat ttccgcctca
      301 attgtggaat accagcagag gtttgccgga tatgatcttc ctcccgtgcg caggataaac
      361 gccgagatcc tttattacaa gctgatgata tttgaacggc ttttcccctt ttatgccact
      421 ataggtataa ttatgcttgt aagccttctg ataatggtta tcgggggcat ccgaacggac
      481 acctttatcc tgaaagcctt gaaatggctg ttactggctg gtttcatcct tcataccttc
      541 ggtatcggat tacgctggta tgtttcaggc cactcaccga tgagcaacgg ttatgagtcg
      601 atgattttta tttcatgggt taccctgttt gccggtttca ttttcagcag gaaatctgat
      661 ttcacccttt cggcaaccgc tgttctggct tccatgaccc tgatggtcgc ccacctgagc
      721 ttcatggacc ctgaaataac caatctcgta ccagtgctga aatcctactg gctcaccctg
      781 catgtttcag ttatcaccgg cagttacgga ttcctcggac tgggtgcgat ccttggcatt
      841 ataaacctta tcctggttgc actatcaggt gaaaggaaca gggagaggat ttccgccacc
      901 gttgacgagc ttacggttat aaattataaa accctcacca ttggccttta ccttttaacg
      961 attgggactt tcctgggcgc tgtatgggca aatgaatcgt ggggaaggta ctggggctgg
     1021 gatcccaagg aaacctggtc gcttatcaca atagtggtct acacccttgt aacccattca
     1081 aggatgatcc cggggctcaa ggatatttat tcattcaacc tgctttcaat gatcggcttt
     1141 ttttcagtgc tgatgaccta tttcggggta aattattatc tctccgggct gcactcatat
     1201 gcaggaggcg atccggtacc ggtaccggct tttgtatatg tttcggttgt tattcttgcc
     1261 ctgctgtcag taactgcata tatgagatat cggaaatggg aaaagggcaa tggatgatag
     1321 tccgacagaa aaggttttca ttcagtttat cggctcatca tgtgttttgt tcattaggtt
     1381 tgtgagggat tgccgttttc gtcccgccgg tgcgtgactc aaacggcgat ctccggggag
     1441 ggtggtccga cagaaaaggc tttcattcag tttatcggtt catcatgtgt tttgttcatt
     1501 aggtttgtga gggattgccg ttttcgtccc gccggtgcgt gactcaaacg gcgatctccg
     1561 gggagggtgg tccgacagaa aaggctgcgc cccggcacac cggggcttca gccttgtttt
     1621 ttattaccgc ttattggagc aagcttcaaa cgctcataaa aaacaaggct gaaccgcctc
     1681 cggcggccag ccttttctgt cggggtggaa gatgggattc gaacccacga ccctcggaac
     1741 cacaatccgg tactctaacc agctgagcta catccaccgt ttcagggtgc aaagtaagcc
     1801 caaaaaatcc atttgtgcaa aaatttgaat ttgttttatg ccaaatctgt ccaattagta
     1861 tctttgcgcc gcggttcaaa tagaatggac cattcttttc aaatttgtac aatacattat
     1921 gtctccagaa agacctttca cggtcagtat cccgaaacta agaaagataa aactgatcga
     1981 caatatctcc gggctgcagc tgttccagct gatgcgcttt acggtctttc ttataatcag
     2041 cattgtattt acgaaaagcc atcttacaag agccgaaata ggatcgtggg agatgttcct
     2101 gtttatttcg ggactgatca gttttttctg ggttacggga atcattcagt cgttgcttcc
     2161 cctttaccac cgaaacaaaa cataccgaaa gattggtgaa aacgggaagg gcaaatcacc
     2221 cgagatattc aatgcattta tactgctgtg cctgttcagc cttgtatttt tcggtatggg
     2281 gcactcactg aagaatcact tttctgtgtt caacatatca ggtaatgtac cctatctaaa
     2341 ccttctcctt ctgtatcttc tcctgagcaa tcctgtctgc ctgattgaat acatttacct
     2401 gttgaacaac cgctcctaca ggatctttca gtatgggatt tacacatttt cccttcagct
     2461 tgtttttgtg ataagcccga tcttgttcag gaaagatatt atatgggccg tttacgggtt
     2521 gcttgcgatt actgcagtaa gatgggtctg gcttataata cttctgagga ggtatacgga
     2581 aatgcagatc tccgtgaaat tcataaggga gcatctttac ctgggatttc cgcttataat
     2641 tacttccctt ctgagcggtt cggcccagta tgccaacgga gtaatcattt ctgcggtgta
     2701 tcgtgatcca ggtatgtttg catggttccg ctacggtgcg aaggagttcc ctcttgtgct
     2761 tatgcttgca aacggactga gcaatgcaat gctgccggag ttttccacca ggtcaagaat
     2821 gaaggaatcg ctcaccagga taaaatataa atcccagcgt ctgatgcatt tgtgcttccc
     2881 tgccacaatg ctgataatgt tgctggcgag gtggttttat ccccggatgt tcaatcccga
     2941 gttccagaaa agcgccgacg tatttcttgt gttttcatta ttcgtcattc cccggctggt
     3001 gttcccccag accgtgatcg ttggaaggaa aaaaacccat ataaccctga ttgccgcagt
     3061 gattgaactt ggaataacca taacactgag cctgctgatg ataaaatggg gctacaacat
     3121 tgtgggtgtt gcccttgctt cgttcatcgg ctatacggtt ggaaaaatat atctttcagg
     3181 atatctgtgg gttaaaatga aaataaaacc tgatgattat attccactga aagcatttct
     3241 gatttattca attctgctcg ttgccgtgtt tgtacttatt gaccacagga taatcgatat
     3301 tcattgattg caggcagccg aagaattccg gcatagtcct ggctcatgta cagtgatgaa
     3361 ttccacaggg aaatcgcgtt gttgaaaaaa cagcggaagc ggttcccgca accggtttta
     3421 aaaatctttt tatatttgcg gtttcggttg atggccagtt tcttcgtatt ggcaatttgt
     3481 tcaaaccata tgcattaaaa aacattatgc aaattatcca ggttacaact aaaagggaca
     3541 gggaggagtt tctgaatctt ccaaggcggc tgtataaagg ggatcccaac tgggtttgcc
     3601 agctggattc ggaactggag gccctgtttg acccggaaag gaattacctg ttcaggcagg
     3661 gtgaggcaaa caggtggata cttaaagatg acagcggcag gaccattggc cgtattgccg
     3721 ctttcatcga caaggtcaga tcagacgctc atttccagcc tacaggcggg attggttttt
     3781 tcgaggtgat taatgataaa aatgccgcct tcatgctttt cgatgcaggc cgggaatggc
     3841 tgacctcaaa aggaatggaa gccatggacg gacccattaa cttcggggag aatgacaata
     3901 actggggact gcttgttgat ggattcatgc agcagggcta cggaatgcca tataataagg
     3961 agtattacag gagctttttt gaggagtatg gattcaggaa ttattttgaa caatattcat
     4021 atcacaggga tgtaagaaca tccgacaata aaatagtgga gtttccgccc cgaatcatga
     4081 aaatagcaga atggctgtca aaacgtcccg ggtattcttt ccgccatttt gaatttgcca
     4141 ggaagcagaa attcattgat gatcttgttg agatatataa tgcaacatgg aaaagtttca
     4201 aggaggattt tacccccctt gatcccgaat tgctcgatgc tttgttcagg aaggcaaaac
     4261 ctgttattga tcaggatata atctggtttg cttatttcaa cgataaacca atagcctttt
     4321 ttatcctcta tcccgatttt aaccagatcc tgaaaagctt aagagggaaa ctgcatttac
     4381 tgaataagat caggttcctt tacttcaagt tgacccataa gatgacaagg atgagggcag
     4441 ttgtcggagg agtgcttcca tcgtaccaga acagcggggt tgaatctgcc atcttttata
     4501 atctttatga agtcttcaaa agaaaaccat ggtacaagga actggaactt tcgtgggtgg
     4561 gagatttcaa tccgaaaatg atagcaattt atgaagccct gggagccaga aaggctaaaa
     4621 cgcacattac atacagatac cttatcaaca aaaagctcaa atttaccagg tataaggttg
     4681 aaatggcagg cattcccgat tcggaaccgg atcagccggg aaacaaaaaa taaaattgtt
     4741 tttaataaaa atttacgtaa ctttgccaac ctttatttaa aaaaggacag ccggatttta
     4801 aaatgaaaga aagatgaaga acggtataca tcccagcaat tacagactgg ttgttttcca
     4861 ggatatgtcg aacggatatt catttcttac caggtcaaca gcaccatcca gggaaaccat
     4921 taaatgggag gacggaaatg agtacccgtt gatcaagctt gagatctcga atacatcgca
     4981 tccgttttat acgggcaaaa tgaagcttgt tgacactgca ggaagggttg ataagttcat
     5041 gaaccgttat aaggatcatg ttggcaggaa aaaatagaag aaaaaaatcc aggatcccgg
     5101 ctaaacgtgc cgggtttttt tttgaattgc agggaatggc acaatgattg ctctggactt
     5161 tcctgacttc cggtgggaaa ttttttgtaa ctttgacagc attttttcat agatggacaa
     5221 gaaactgagg cgaacggtta agagtttttt tattccggat ctcatggatg gcaccggaga
     5281 tatcgttcct atcattgcag atggggagga tggtgatctt gaggatgttg aggtgccgga
     5341 aaccattccc gtactttcat tgagaaacac agtacttttt ccgggagtgg tgattccgat
     5401 atcaatcgga cgtccgaaat caattcagct gataaaggat gtttataagt ctgacaggat
     5461 aatgggaact gttgcccagc gggaccctga tactgaaaat cctgcttttc ccgatcttca
     5521 taccgtaggg accattggtc agatagttaa gctccttgaa atgcccgacg gctccaccac
     5581 ggcaataatt cagggacgga aaaggatggt attgcaggaa ctcgttgcag acgaccctta
     5641 cttcatagcg cgtgtcaagc ctatccccga gaccaggcca gagctcccga gcaaagattt
     5701 cgatgctgtt gttggatcgc tcagggactt gtctctcaag ataataaaaa tcaatcccaa
     5761 tatctctccc gaggcttcat ttgcaatcaa gaatatcgag aacagcacct atctcataaa
     5821 cttcatctgt tcaaatacag atataaaagt tcaggataag cagcgccttc ttgaaataaa
     5881 caatctccgc gaccggggct atcagttgct ggaatacctg gtgcaggaga tacaactact
     5941 tgagctgaaa aatgaactcc agtcgaaggt gaaaagcgat ctggatcagc aacagcgtga
     6001 attccttctc caccagcaga tgaaaacgat tcagaatgag cttggcggga atccgcttga
     6061 gaaggagata gaggaataca ggaagaaggg agagtcaaag gactggccgg ctgaggtcag
     6121 gaagacattt gaaaaggaac ttgataagct gaacaggctc aatcctgcag ctgcggaata
     6181 ttccgtccag gtgaactata ttcagaccct cctggatctg ccatggaact tatgtacaga
     6241 agacaatctc gacctgaaca atgccaggca tgttcttgac aaagatcatt tcgggctcga
     6301 ggaagtcaag gaaaggattc tggaacatct ggccgtgctt aaactgaaag gagatctgaa
     6361 atcgcccatt ctctgtcttc acggtccgcc gggagtcggg aaaacatcac tcggaaaatc
     6421 agttgccaag gccctcggcc ggaaatatgt aagaatgtca ctcggaggtc ttcacgatga
     6481 ggcagaaata agaggacacc ggaaaacata tatcggtgca atgccgggca ggatagtaca
     6541 gaacctgaaa agagccggat catccaaccc ggttttcatt ctggatgaga tcgacaaggt
     6601 tggacaggat ttccgtggtg atccctcttc agccctgctt gaagtgctcg atccggaaca
     6661 gaacagcacc tttttcgaca atttcctgga gatggaatat gacctttcca gggtgctttt
     6721 cattgccacc gccaataatc tcgccacaat cagtcctgct ttaagggaca ggatggaact
     6781 catagagatc accggatatc ttgtggagga gaaacttgag atagcagaac ttcacctgct
     6841 tccaaaacag attgagaacc acggagtaag accggatcag atttccatca gcagaaaggt
     6901 gatggaaacg attattgaaa aatatacgag ggaatcggga gtgcgggagc ttgacaagaa
     6961 gctggccaag atactcaggg ttacagccaa gaatatcgcg tcgggcatca gctatgaaac
     7021 tgaattgtca gagaaaaagg ttattgagat tctcggaccc ccgaaatatg tacgcgacat
     7081 ctatgcgggc aatgaacagg ccggggtggt tacgggcctt gcctggacag cggcaggcgg
     7141 cgaaatcctg tttgttgaaa cgagccttag ccgcggaacc ggaaaactta cactcaccgg
     7201 caacctggga gaggtaatga aggaatcggc cataattgct ctcgaatacc tgaaatcgaa
     7261 tgcccggctg ctggcccttc ctgacaactt ttccgaaaaa tgggacatac atatacatat
     7321 cccggaagga gccattccca aagacggccc ttcagccggt attaccatgg caacatcaat
     7381 tgcatcggca tttacgcagc gaaaagtgaa aaaattcctt gcaatgacgg gagaaataac
     7441 tctcaggggc aaagtgcttc cggttggggg aattaaggag aagatccttg ccgccaagcg
     7501 ggccagtatt aaggagatca ttatatcgga agacaacagg aaggatgttg aagatatcaa
     7561 tgagatctat atcaggggac ttaaatttca ttatgtagac acgatcatgg aggtgttgga
     7621 actttccctg ctgaggcaga aagttaaaaa tcctaagaaa atcacatttg aatgaaaaaa
     7681 attgccgttt ttcccggttc ttttgacccg ttcactattg gtcatgaagg aattgtaaaa
     7741 agggccctta acctgtttga tgaaataatc attgcggtgg gtgccaacgc cctgaaaaaa
     7801 aactattatc ccgttcatgt gaggaaggaa atgataggaa gggtattcca ggatgaaccc
     7861 agagttaagg ttgatcatta tgagggactt acggttgact actgcataag gcataatgca
     7921 cgctacatcc tcaggggact caggacagcg gcagactttg aatttgaaag agccattgcc
     7981 caggtaaaca aggcactggc agcggatgtt gaatcagtat tttttcttac gatccctgag
     8041 cattcaccca taaattcctc catagtgagg gacattatac gcagtggcgg ggatgcttcc
     8101 cgcttcgtac cggctgctat taaactgaaa gattacaagg gagaggaaga atagtatttt
     8161 ttttaacggt cagtctttca ggatggatat gtttataagg tccatgagcg aatgccatgc
     8221 acctgccttt aaccggctca cttcttcctt tgtcagccac tcagccttgg tgatcccttc
     8281 ctcaagctgg ggtttgatgt ccattgtacc tgaataatcc atcaggtacc acctggtgtt
     8341 tttaaggtac gaaatgccgc ggcatgtata ggtatggtag gtggaatcaa gaaatctggt
     8401 tattttatgt ccggttattc cgcattcttc ctgaacctcc ctcaaggcac atgtctcggg
     8461 atcctcgtca ctttcaatat gaccctttgg cagatccagc cttccctttc tctcaatgaa
     8521 cagatatctg cccgaagaat gccttaccag cccgccagcc gccccgactt cgatgaaata
     8581 tatgcggaaa atcctccaga tatgtcttat gttggttccg aaaatattga tcgaatagat
     8641 tgaatcatcg gcctgaaaat cggaaataag cttatatagt tcctttgtgg caccgaattt
     8701 atagaataac ccgtattttt gtaaccggtc aggttcggca ctgatcaata ggaatctgtc
     8761 ttcgaagtga accttataca ttctgaaatg gaagattgtg caacaaagat cgctgattat
     8821 ctgttacaaa ttaaagcaat aaaattgcaa ccgtcaaatc cgtttacctg ggcgtcggga
     8881 tggaagtcac cgatatattg tgataaccgg aaaacattat cctttccgga agtaagatca
     8941 tttatcagtg agaaattcgc ggaatatgta aggaatctgt atcctgatgc agagattata
     9001 gccggagttg caacaggtgc cattgcccat ggggtcctca cggcggataa actgaacctg
     9061 cccttcatct atgtgcgctc gggcgcaaag gagcatggcc tgggtaatca gattgagggt
     9121 tatttcgaac cgggccggaa agttgtggtt attgaggacc ttgtttccac cgggggcagc
     9181 agcctgggtg cagtcaaagc attaagggaa gcaggatgtg atgttttggg aatgctggca
     9241 attttcacct atgaatttga gagggcagca gacgggtttg ctgcccaggg ttgtaagctt
     9301 gacaccctcg gcaattacag tactttaata gaaacagcac tttcttcggg ttatataggc
     9361 gagtctgaac tggagacact gaaaaggtgg agaacagatc cctcaggctg gggtcagaaa
     9421 tctgattaaa tgggacaatc agcagaatat acaagcaggg aaggagtttt gacatgcact
     9481 cccagggaga tatttgaatt tgcagccgat atgggcaaca tgataaacct gctgccaccg
     9541 gacactgcaa aaataattga atcgaccggc gaatcctgtg ccctttcatt tgacccgctg
     9601 ggcagcatga aaatacaaat aatcaggaaa accccgtatt ctgaggtggt ttacagggct
     9661 gaactgctgg atatccagaa tctcctgctg aacctggaga taaccggagg agattctgaa
     9721 aaagcaaaag tcgtcataag ggtaaatgca gaaatgaatc cgttcatgaa aatgatggca
     9781 gaaaagcata ttgccaggtt tcttgacatc ctgatcctgg aaatggagaa attcagggac
     9841 tggaaaaaat cataaaatat attcaatttc ctttgtgcca aattcaaggc ttttaccggc
     9901 tctgtccacc acggtcagat tacctctgcc agtgaccgca gctatttttc cctcaaagat
     9961 catatcacct gccctgtaac tgtgccattc attcagccgg tacagcaggg aggagaaccg
    10021 ggacctgata ttttcatgat ctgctgccag aagctgcttg taacttttat ccaggttttt
    10081 tatgagctgt ttaaggcata tttccggatc gtattcctcg cccgtaagca ttttaagcga
    10141 aaccgggtta ggggctccgc tgatgaatct tgtctgattc aggttaagtc caatgccggc
    10201 aatgctacta cctatctttc cgtcgagaat cgagttttct atcaatacac ctgcaatctt
    10261 tgaatcaccg acataaatgt cgttgggcca tttgattttg catccatgga ccttctcaga
    10321 aataaaatca tatatgccaa gcgatattgt catggaaatc acgtactgat cttccggggc
    10381 taccatgccg ggaaacagaa taatgctaca caatatgttt tttccggctt cgctttccca
    10441 tttgttttct ctctgacccc ggccggccgt ctgataatca gtatatatta ccgtgccttc
    10501 ggcaggaacc ggatctttct tcaaaagacc tgaggcaaac gtattggtcg atgagatgtt
    10561 ccggataaaa attattttgg agccaattat cattcttgtg atgcttcagg agggtttgac
    10621 gggcttcatt tcttaccggg ctggtactta aattgtaaat tcagggctgc catacaaaaa
    10681 tacggataaa agtttgtact tttgcgactt gatatttaat tgatagttac ggatgaagaa
    10741 aaggcgggac gaaaccgaat tgctgcttga aaatattata agggggatag ttgaaaagaa
    10801 agggaaagat attctgaaaa tagatttaag gggcctggaa aacaggatca ccgacttctt
    10861 tgtgatctgt catggttctt cagtcacaca ggttgactcg ataaccagtt ccatagagga
    10921 cactgtgcga acaggtacgg gggagaagcc cggacatatt gaagggcttg acaactgttt
    10981 ttgggtcctg cttgattacg gaaacgttgt tgttcatgta tttcttgaag aatacaggag
    11041 tttttacagc cttgaatcac tttgggctga tgcttccctg gaggcaattg aagacaatca
    11101 gataaattat taatttatgg ctgaaaagaa taataaaaat aataatccgg gactggggaa
    11161 cagttccgac aagaacgcaa agcccaggtt caattcaaat tggatttttg cgatccttgc
    11221 cttttccata attgcttttc agtttttata tggaggcaga tcggtaccca aggctcagac
    11281 aagcgacctt aagaaaatgg tcgagaacag ggatatagag aggattgttg tcgttaataa
    11341 ggaggttgcg gagatttacc tgaaaaagga ggcaattgag tcggacaggt atccaaatct
    11401 taacaagaac ggcagcagaa gcatcgggct tagtactccc aaacccaatt tcacatataa
    11461 tatttcggat ataagcatct tttacaaatt tctcgaggat gagcaaaaga aagccggata
    11521 tgaggagaaa gatgttattt accctgagtc ggaaaccagg cgcaattata tcggcgatat
    11581 cctgggatgg accctgcctt tcatactgct gatattcctg tggatataca tgatgaggcg
    11641 aatgtcggga ggtggtgccg gcggtgccgg aggcattttt agcgttggaa aatcgagggc
    11701 acagattttc gacaaggata ccaacgttaa gataaacttc aatgatgttg ccggtctgga
    11761 ggaggcaaag acagaggtaa tggaaattgt ggatttcctt aagaatccga agaagtacac
    11821 acagctcgga ggaaaaatac ccaagggagc tcttctcatc ggacctccgg gaacgggaaa
    11881 aactcttctt gcgaaggctg tggccggaga ggcaaatgtg ccgttctttt caatatccgg
    11941 atcggatttt gtggagatgt tcgttggtgt gggagcttcg cgggtcaggg atctgtttaa
    12001 acaggccaag gaaaaggccc catgtattgt gtttatcgat gagatcgatg ctgttggcag
    12061 ggcaaggggt cgtaatgtgg gtttcagttc aaatgacgaa cgcgaaaaca ctttaaacca
    12121 gttgctcacc gaaatggatg gcttcggaac caatatggga gtaattattc ttgcagccac
    12181 caacagggca gacatactcg acagggccct gttgagggca ggaaggtttg accgccagat
    12241 acatgtcgag cttcctgatc tgaatgagcg tgaagcaata ttcaaggttc acctccggcc
    12301 gattaaaatg gagaagaatt tcgatatttc cttccttgcc aagcaaactc cgggcttctc
    12361 gggagcagat attgcaaacg tgtgcaacga ggcggctctt attgctgcaa ggaaaaataa
    12421 gaatatagtt gagaagcagg atttcctgga cgccattgac agaattgtgg gcggactaga
    12481 aaggaaaaac aagatcatct cacttgaaga gaaaaaaacc atagcatacc acgaagccgg
    12541 gcatgctact gtaagctggc ttctggaaca cgccagcccg ctcctgaaag tgacgatcat
    12601 accaagggga agagccctgg gcgcagcctg gtatcttccg gaagaaagac agataaccac
    12661 ccgggaacag ctcctggatg aaatggctta cgcccttggc ggaagagctt cggaggaact
    12721 tgtctttggt aaaatctcaa caggagcact gagcgatctt gagaaaataa ccaagcaggc
    12781 atatgccatg gtctctttct tcggaatgag tgagaaagtg ggaaacatca gcttttatga
    12841 ttcttcagga caatcagatt tcggctttac caaaccatac agcgaaaaaa ctgccgaact
    12901 gatcgaccgg gaagttaaac tgataattga agaatcgtat caacgtgcaa aagatgtcct
    12961 tagtgccaat atgaaaggac tgaccgagct tgccgagctc ctgctggaaa aagaggttat
    13021 cttcagcgaa gacctggaaa ggattttcgg aaaacgcaag ggcgataagc tcaaagccga
    13081 atcagaagcc caggcaaaga aagagacaga agctgaaccg gacctggttg agatcaataa
    13141 ggaatccggc gaaaataaaa ccggcagaaa gaagggaaaa acggcatcac agaagaatgg
    13201 ggacattcct gaccccggaa aatctggcta aaataatcag gaaggcaaga tatcattaac
    13261 accggaccac attgagcaat tttttcagac gtacccttac ggcaatatgg attgtcatct
    13321 ttgttctggg cggattctgg ctgcacccgg tttcattttt tctgaccggc ctgataatag
    13381 ttgcctgtac acaatacgaa tattactcga tgattaaata taccgggatt cggccccagg
    13441 tggcaggagg aattactgcc ggactgtcgg catttacagt gtccaccatg gttgcagccg
    13501 gattgttgca aagtcgctgg tatctgcttt taatacccct gatattgatt ctgatgatag
    13561 ccgaactttt cagaaaaact gaaaagccat tcgattcgct tgcccacact gttttcggca
    13621 ttatatatgt tgcactgcca ttttcacttt tcccttctgc ggcttttgcc cgtacaggcc
    13681 tgggttcgct gataccgcat cagggaataa ttttctcccc gggtttcatt gttggctttt
    13741 ttcttgttct ctgggcaaat gataccggcg cctacattac cggagtgtca ttcgggcgac
    13801 acaggctact ggaaagaatc tcacccaaaa aaacatggga gggttacctg ggaggccttg
    13861 cactggcaat ctttgcggcg tggctgttgg gtgactggtt tggagtgatg aagaaatcac
    13921 actggatggg gattgcagtt attgtatcag ttgccggaac cctgggcgat ctgttcgaat
    13981 ctctgcttaa aagaagtgtc ggagtaaaag actcaggatc agtgatgccc ggtcatggcg
    14041 gtttcctcga ccgtttcgac agcgccctct tcgcatttcc tctcggatat cttttccttt
    14101 ctctttttgg atgattgatg cgtgatgatt agttttgtgg aaattttgct ggaatgggaa
    14161 ttcacaaaga gggatataag atattgattt acggatttat aatgctggta tttgtcaatg
    14221 tccttgcagg attgatatgg cctgatatgg ttcttttgag atatactgtg cttattattg
    14281 cgtttttaat gtatatattt cttctattct tcttccggct ccctgtaagg tatcttgaac
    14341 ccgatcctgg ccttatttat gcacctgccg atggtaaggt tgtggtaatt gaagaaaccg
    14401 aggaaaagga atatttcagg gatatgcgcc tccagatctc tattttcatg tcaccgttca
    14461 atatgcacag caacaggtac cccgtgtcgg gaaagatcaa gtatgtcatg tatcacccgg
    14521 gaaattatat ggttgcctgg catccgaaat catcggagat gaatgagcgg agttcaatag
    14581 tcatagaaac tgaaaaggga gcagaaatca tgataaggca gatcgccggt gcggttgcca
    14641 gaagaattgt gacctatgca aaaacaggag cccatgtcac gcagggcgat gaactcggat
    14701 ttatcaaatt cggttcaagg gtggatgtct ttcttccgtt gggcactgag gtggaaattc
    14761 ccatattgca gcaggttaag gctaacaaaa gcattattgc aaaaatctga acggaattat
    14821 atcatgaaac aaaaagatga caaaataatt gaccttaccg aacatgtcaa atggatcgga
    14881 attcttgatt atgatataag gacatttgat atcgtgatga ataccgaatt cggaacaacc
    14941 tataattcct tttttataaa tactgaaagg aaaacgcttg tggatgttgc taaggagaag
    15001 ttcagcgagg tatggtttga taaactcaga gccctgactg atccatccga catcagttat
    15061 atcgtgatta atcatacgga gcctgaccat tcgggaagcc tgggaatgct gctggacgcg
    15121 gctccttcgg caacggttgt gggaagtggc agcggcataa gatatcttgc agatatgatc
    15181 gccaaaccat tcaattcgat cgttgtaaaa gacggggaca cccttgacct cggatccgga
    15241 aaacttaaat tcatttctgc acccaacctt cattggcccg attccatgtt tacgtggttt
    15301 gaagaagaca ggattctttt tacctgtgac gtatttggtg cacactactg cgacagcaga
    15361 atgttcagcg acattgaatc gcaggattac atgaaagcct acaaatacta ttttgatgtg
    15421 atcatgaaac cttacagcag gtttatgatc aaggccattg agaagatacg cgatctggac
    15481 ataaacctga tatgcccggg tcatggcccg gttcattcgg aaaacctccg ggatatggta
    15541 atgctttccg agaattatgc aatagaatac ctgaagcagg tcgaatctaa agatgaaaag
    15601 aaaatcctcg tagcctacgt ttctgcttat ggttatacga aacaggctgc cgatcttata
    15661 gcatccggta tgcgtgaaac gggtgatttc agtattgata ttgccgatat tgaaaccatg
    15721 ccggcggaag agctggaatc gaagctggtt tcttccgatg cgatcctgat cggatcacct
    15781 acaataaacc agaatacact actaccggtt tacaggttat tttcattgat caatcccata
    15841 cgggataaag gaaaaatggc cggggttttc ggatcatacg ggtggagcgg agaggctccg
    15901 aaaataatat cagaaactct gagaaatctc aaactcaggg tttttgacga aacagcggga
    15961 ttcaagtttg ctcccggaaa tggtaaaaaa gaaaccctga cggaattcgg cagacaattc
    16021 gccagggaat ttctcaaata ttgctagcag gctggttata aaatccgcat cagtttaatt
    16081 tactgctgat tgtaccgatc ggccggccgg tcaatgaccg ctgaacctcc tggttcactt
    16141 gttgacggcc ttcatgaaca tgatgccgtg agtaacttaa tcttcaggaa acttttacct
    16201 gcctggtcag cttatgtttt tcctcgtcat atttgggtag ggtaatgttc aggattccat
    16261 ccttatagct ggcttcaatc ttatccttgt ttacactatc aggaacctgg aaacttctgc
    16321 agaacgatga atagctgaat tcccttcttt tatagccatt gccgtttttt tctgattcat
    16381 tctttacctc atgcgatatt gtcaacaggt catcattgag atctatctta atatcttttt
    16441 tgttaatacc cggtattgcc acgtcaagtg cgaagctttt ttcattctct cttacgttaa
    16501 ctgccggaac tgacctgttg aggcttgtcg ttgcaggtaa aaagtcgtca tcaaacagtc
    16561 cgttcagaaa aaaaggcctg tagcttcttc ttgtaattat tggtaacatg atttggtcct
    16621 ccgttatttt tagttgaaca ttatttacgt tttgctttag tattttcaac ttccgttcca
    16681 taatcaaaaa cacgccatat tgacacgaat aaagtaaata aaccgacata ttgacatgat
    16741 aaataagtca atataaaagt cggattacag taggaacagt tgaacaggaa tgtatgattt
    16801 tagggaattc caaaaaatta gcataggttt gtataggaaa aataaaacca atgaaagcca
    16861 cacttaggaa cttcctcata ttttttggag tattgattct tctggctgca gtctggtact
    16921 ttcgcaatat agttgtttac atactggttt ccggtgttct ttccattatg ggccggcccc
    16981 tcgttgatct tttttgcagg ataaggatct ggaagtggaa attcccgagg gtattggctg
    17041 cccttttcgc attgatcatt atctggggtt caatccttct cttcctgtac atattcatac
    17101 ctcttgttac caagcagata aatcacttta ccggcattga cagtgaaaag atagtcagac
    17161 tgattgaagg cccactggaa aaaattgaaa agttgtttgt cagcctcaat cccagtatat
    17221 ccgacacctt atcgctcaag gcatatattg catcaaaggt atcggaagtt ttgagcatcg
    17281 atatgattca ggattttatt ggatcgatta tcggtgttct cgggaatgtt gttgttgcaa
    17341 tattttcaat aagcttcatc acgttctttt ttctcaagga tcagcatctt ttcttcgaat
    17401 cgattctcat gtggattccc gaccggtatg ttgataatgt gacccgggcg cttaactcga
    17461 taaaaaaact gcttacaagg tatttcattg gaattgtcat tcagagtaca tgtatcatga
    17521 tccttatcga cataggaatg acaattatag ggattgagtt ccatcaggcg ctggttatgg
    17581 gtctgatcat cggaatactg aacgttattc cctatgtggg tccgtggctg ggattattca
    17641 tagcaataat aatgggagtg gcttcacata ttaaccagga tttcaacact gtggtgattc
    17701 cactggtgta ttacatgatt attgttgagg cagttactca cactattgat aatgttgttt
    17761 ttcaacccgt gattttttcg agcagtgtca aggcacatcc gcttgagata tttgtggttg
    17821 tcctggcagc cggttttgct gccggggttc cgggaatgat cctgggcatt cccacctaca
    17881 cagttttgcg ggtttttgcc agggaatttt tctacggatt caaggcggtg cagaaaatta
    17941 cctccagcct gtcggatgag aactctcctg aaaaaagcag gatatcaaat gttctgattg
    18001 aagaatcaaa aagattgacc acgaggaaaa ggtcagggaa gaaatcccct ggtaaggcgg
    18061 aagaataagc tgaatgtaat gggatcatca cctgtgctga taaagagagt gttcagattt
    18121 tactatgacg ggttcagaag catgtcgtgg tggggtaagc gcgtgtggat cataatactg
    18181 ataaagcttt tcattatttt tatcattttg aaactgtttt tcttcaggaa tttccttaat
    18241 gaaaaatttg ataatgagaa acaaaaaagt gagtttgttc tggatcagtt aattaattca
    18301 aaataaacat atgattgatg ttcccgacct gacccttgta gattggtcaa gggcacagtt
    18361 tgccctgacg gcaatttatc actggctttt tgttccgctt acactgggac tttcctttat
    18421 agtggccatt atggaaacta tatatgtaaa gaccggcctt gaagaatgga agcgaatgac
    18481 ccggttctgg atgacgcttt tcggtattaa ttttgcaatc ggcgttgcta ccgggatcat
    18541 ccttgagttt gaatttggca ccaactggtc ttattattcc tggttcgtgg gggatatttt
    18601 cggtgcacca ttggcaatcg agggcattct ggcctttttc ctggaatcaa ctttcgtcgc
    18661 ggtgatgttt ttcggttgga acaaggtaaa caagaagttc cacctggttt caacatggct
    18721 ggtggctgtc ggtgccagcc tgtcagcatt gtggatcctt gttgccaacg catggatgca
    18781 aaatcccgtg ggaatggttt ttaatcctga aactgcaaga aatgaaatgg tcgacttctg
    18841 ggcagtgctt ttcaatgaag ttgcagtcag caagtttctt cataccatat cttctgggtt
    18901 cctcctcgga tcaatgtttg tggcagggat cagtgcatgg taccttcttc agaaaaggga
    18961 acaccacctg gctacaagga gtcttcttgt ggcaggggtt ttcggactgc tatcgtcact
    19021 gcttgttgtt tataccggcg acagttctgc aagaacgctt gcacgtgcac agccagttaa
    19081 atttgcggct ttcgaggccc tctatgatgg cagatcggat gccggacttg ttgccgcagg
    19141 tattctgaag gattccgaca ggaagattgg aaaaaaggtt gtcaaggatt tttctttcag
    19201 gattgaaatt cctggtttcc tctcattcat gaccggcgga aacacaagag catttgttcc
    19261 cggaataaat gacctgatcc tcggaaaccc ggaaaaagga ctacttcctg ttgctgaaaa
    19321 gatgaggagg gggaaggtgg cacaggatct tttgcgcgac tacaagaatg ccagggcggg
    19381 caatgattca gtaaaaatgt cgacgataag gaaagttttc gccgataaac aattcattga
    19441 agaacatttc agatactttg gctatgcttc ggttgaaaat ccggaagatg caatccccaa
    19501 tgtcccgctt gccttctatt catttcatct tatggtcact cttggatttt attttattct
    19561 gcttttttgc cttgtacttt ttttactttt caggggcacc atagcaaaac aaagatggtt
    19621 cctgtggata cttcttttct cgatactgct tccttacgtg gccggagaaa tggggtgggt
    19681 cctggcggaa atgggccgcc agccctggat aatccaggac ctgatgcctg tcagcacggc
    19741 tgtttcacgt attggatcag gatcggttat aaccactttt gtactgtttg ctgtactttt
    19801 cactgtcctg ttgatagccg aagtatcaat aatgataaaa cagataaaga caggaccaaa
    19861 tcattaggag gaaataacca tggaaccatt gatatctcat ctttttctgc agcattactg
    19921 gtggataata atctcaattc tggcgggact gctggttttt ctcctgtttg tccagggtgg
    19981 tcagtcaatg atattttccc ttcctgagga tccccttcag acaacgattc tcgtcaatgc
    20041 tcttggccgg aaatgggaat ttaccttcac aacactcgtt acctttggcg gagccttttt
    20101 tgccgcattt ccccttttct attctgccag cttcggtggt gcgtactggg tctggatggc
    20161 aatcctgttc agtttcatta tccaggctgt tgcctacgaa ttcaggtcaa aaaagggaaa
    20221 tttccttggg caaaggacct atgatatttt cctgcttatc aatggatcac tcggtccttt
    20281 cctgcttgga gtggcagtcg ccacattttt cacaggggca cggttcagtc tgaatgaact
    20341 gaacagggtc agctgggcaa caggctggca tgggcttgag gccctagtga acggacgcaa
    20401 tgtcgccctg gggctcgcag tacttttcct tgcacgaacc aatggcctcc tgtatatcat
    20461 taattcggtg aatgatgatg gactttatga gaatgcaggc aggaaaataa tactgaatgc
    20521 cctgactttc ctcgtattct tcctgttctt tctcgttaca ttgcttatat ctccgggatt
    20581 tgcctatgat ccgcatacgg gaaatgtttt tatcgaaaaa tacaaatatc ttcataacct
    20641 cctcaggatg cctgccgtat tgattatttt tgttgcaggg gttgtcggag tgctttacgg
    20701 gatagtaatt acggttttca gggggagccg taaagggatc tggtttaccg gaacaggaac
    20761 aatacttgca gttatttcgc ttttcctcct tgcgggattc agcaacacag ccttttatcc
    20821 ctccacattt gacctgaaca gctcattgtc gatccgtaag gcctcgtcaa gttatttcac
    20881 actgaagaca atgatgttcg tttcattcat tattcctttc gtggcggctt acatatggta
    20941 tgcatggaaa gctatcaaca ggaggaggat ttccgtggag gagatacagg aagaaggaca
    21001 tgtttactga acaaataaaa gtgaacgata aactattaac gacaaacggt aaaaccctca
    21061 aatcaacagg ttgaataaaa attgcattgt tatgtatttc acaggtctgg ctcactatct
    21121 gatctggccg gcattcatcc tggtcagctg gtttattatt aaaggcgccc tggcagttta
    21181 tgaaaggaaa ttccccgggg ataaggaaga aagctgaccg gccaggtttg ctgatggccg
    21241 tattccctgg atgacatgcc cgtgcaacgt tttcacggac attgaataca aatggatgac
    21301 attttatatg accgccgcta gccttccgtt ttcccggcaa acatgggaga gattagtgag
    21361 gctataatat aaaacggaat aaaaagggga gctgatgcta tccccagaag gattagggat
    21421 actatgacca ggccgaaaag tatgtactgc ccttcattgc cctttatgcc cagattcgac
    21481 attttcagtg atatcagtgg gattcctgat accatcatca atgacaatac aatcatatat
    21541 accgtaacag caacaggaga tttcaggaac aattcaaaaa cagctgcgtc ggaataatga
    21601 gctgcaataa caacagagat cacggcaagg gcatttgcgg gagtgggcag acccttaaaa
    21661 gacctggtct gggttgtgtc cgtattgaat tttgccagtc tgagtgcggc acaaacaggc
    21721 attaagacag ttatatactt cagagttccg ggattgcaaa agtaactgca cgagtcagct
    21781 ccggcagtgg acccgtatga aatcagttga aatacaatca tggcaggggc aatcccgaag
    21841 ctgacaatat cagccaggct gtctagttct ttccctatat cggagtaggc ttttaaaagc
    21901 cttgcagaaa aaccgtccag aaaatcaaaa accatggctg ctgcgataag ccatgaggaa
    21961 actatcagat cgttaccggc cgcgaaaatg atggcgatga atcccatggc cagattcagg
    22021 catgtaatca gattcggaac atgttttgtt aatgtcattt tgtataaggt ttatgacgat
    22081 gacaaaaata ggtatattag gcattacatt ttgatcgtcg cggctgattt gtttcctgat
    22141 ttattctcat ttgttcagga ttatggatgc gtaattcaaa ggttatctct tttttattag
    22201 atttgcaagt attaatgcag gcctaatgat caatttcaag aatgttaaga tagctgttga
    22261 ctttgatgga accattgtag atcatgaata tccggatatt ggtaaagaaa agttatttgc
    22321 tttccagaca ttgaaagagc tcaataaact tggcgcacgg cttatcctgt ggactttcag
    22381 aacaggcgat gaactggaac aggcagttga gttctgcagg cggaacggag ttgaatttta
    22441 tgccgtaaac aaaaattatc ccgaggaggt atatgatgag tcgataagca ggaaaataga
    22501 tgcggatatt ttcattgatg ataagaatgt aggcggtttt ccgggatgga gtgagatctg
    22561 gcagatactg gttccgtttg aacttcatga aaaggaaatg gaacggcaga tagccgcagc
    22621 caggaaaaaa agcattcgca gattgttcgg aaggaaagcc taaaaagcaa cacaatggca
    22681 agggctgcat ttagatatgt ttttattctg attattctgc ctttactgtt gttcgtatat
    22741 tgttccggac gcaccggagg agcaagggaa aacgggaaga aaacaacttc acgatctgcc
    22801 gtcagtcctt ccctgagatt catcaggatg cagaatccgg tcgaaaattc tgaatttcat
    22861 atcggcgaac aatttgagat aacacttgga tcggaaaata agaaccaggt acccgattca
    22921 gtcaggtttt tttacgacgg gcacattgtg gggcagcttt atgccgaacc atgggtttta
    22981 tcaataccgt ctgctgccgt atcagcaaca ggccggaaac ctgttaaggt aacagcctat
    23041 aaaaacgata aagtacagac ggttacgaga tttgtgatta ttctgtcgga tatagtccct
    23101 gaaacaaaga gtttcaggat cgtaaatacc tatccgcatg accgggaagc ttttacccag
    23161 ggcctttttt ttgatggaag tgttttgtat gaaggcacgg gacaggaggc aggatcttca
    23221 ctgcgagagg tggatctcga aaccgggaag gtaatacggc agcataacct tgagcctccc
    23281 tatttggtga gggtatcacc ctttataagg acaggattta ccaggtgaca tggaccagca
    23341 agattggctt cgtttatgac agggcaacat tcaggttgtt gaacaggttt tattatcaga
    23401 cacagggatg gggcctgaca accatgaatg gaatgatagt catgagtgat ggttcaaata
    23461 acctgtattt tcatgaaccg gagatgttca gtttcgtttc cgggatcgaa gtatatgaca
    23521 atgaaaaaaa ggttgatcag cttaacgagc ttgaatatat taatggtgag atctgggcca
    23581 atatctggca gaccgatctg attgcgcgca ttgacccgtc gacgggaaag gtaaattcct
    23641 acgtagatct tgaatcaatt tatcctgaat ggcaaagaag gcaggataat tcggatgtgc
    23701 tcaatggtat agcgtatgat gcacagaata aacggatctt tgttaccggt aaaagatggc
    23761 caaagcttta tgagataagg gttagcgaat gatccgtgat ccgtagacaa tatttatggc
    23821 gccgggaagt atccggaatt ctgcgggagt atgaccgagt gattccccgt cagcttcaat
    23881 atacagcaac gaatctgatt ggaccgacag gttggttgaa cggtaaccgt caactttcgg
    23941 gtggctgaga atggtaccat catagaggag cttcagactt ttaattacct caaacctgct
    24001 catttccctt attacggtta tatccaggag gccgtcgtca ggcagggcat cgggtgtctg
    24061 cctcatcccg cctccgcaat atcttccgtt tccgatattg atcgaaaata ttttacagct
    24121 gttttttata ccgtcgatga tgatatcggc attcctgtgc ctccatgaca gcaggctggc
    24181 cagcagattg aaaaaatata ttgccttgct gctttttccc ttttccttct gcctgttcgt
    24241 ctttattacc acaagtgatt cgaagcccag tccggcaatg tttataaaat accgtctttc
    24301 cctgttctct ccgttaaaat aatcgataac tccaatgtcg tggggcatct gcctgccttc
    24361 cattattacc ttcaccgcgc cttcataaac cagaggtatg gaaaacatgc gtccccagtc
    24421 attgcctgtt cccacgggta tcatgccaag aagcaactca ctcggggtac agtgctcctg
    24481 ggtgaaagcg ccgttaacaa tttcattcag tgttccgtcg ccgcctacag aaataaattt
    24541 cctgaatccc ttcccgattg cctccctggt atattcggtt gcatgaccct ttttttccgt
    24601 gaacctgact gtgacgggga tggcattttt tgccagtaga tcatttatcc tgtcccagtc
    24661 cttccggccc ttgccgtttc ctgcgttggg attgacaatg gtgaaccagt tattggccgt
    24721 taattctttt ttcaatgccg gttaaagtat aaggtgagtt ttaacagcaa atttaaaaaa
    24781 aataccatgg gtgggcatca tgttctatct cctggttttg catgcaatta ccggacaatc
    24841 atgtaagtat gtatttatgg agcaccgacc gatgttaata agatgttgaa aacgacaaat
    24901 aaaatgttta caattacgct gttcacaggc tgagcctgat actcataaat agtaaatttg
    24961 aagctcaaaa agggagctat attatattgg aaaaataatg ggcagaataa tcgcgatagc
    25021 taatcagaag ggtggtgtag gaaagacgac tactgcgatc aatctggcgg caagtctggc
    25081 tgcacttgag aagaaggtac tgattgtcga tgctgaccca caggcaaatg caacatcggg
    25141 tatcggtatt gacacacgtc agataaaatg cactgtatat gactgtctga tagacggacg
    25201 tgatccttcc gaagccttgc ttggctgcgg tatagataat ctttctgtgc ttccgtcgaa
    25261 tatcgacctg gtgggagccg agattgaaat gcttgaaagg cccgagcggg aaaagatatt
    25321 aaagaatatt ctcggcagga tttcgggaaa ctttgattat attatcattg actgttctcc
    25381 ttcgctcggc ctcttaaccg tgaatgcact taccgcagcg aactcggtta tcattcctgt
    25441 tcagtgcgaa tattttgctc ttgaaggtct gggaaagctt ctgaacacta taaagataat
    25501 tcagaacaat cttaatcccg accttgagat agaaggattt cttcttacaa tgtatgattc
    25561 aagactgaga ttatcgaatc aggttgccga ggaggttaac aagcattttc agcagatggt
    25621 tttccagaca atcatccaga ggaatgtcaa gctctcggaa gcgcccagct tcgggcagcc
    25681 tgccatactt tacgatgctg attcaagggg aacggtaaac tatcttaatc ttgcaaggga
    25741 actgattgaa aaacaggaag tccgggttaa tgcctgaatg gccgttgttg tcaaccgtaa
    25801 ggaggattta taatgacaaa aaagaatgca ctgggcaggg gcctcggcgc cctgattgat
    25861 ggtgttgaaa aagaagttct tgaacggaag gttgaggcca atctcaacat ccccgttgat
    25921 cttatagatg gtaatccttt ccagccaagg gctcattttg atgaacaggc tcttggggaa
    25981 cttaccgctt caatcaagaa gcttgggctg gttcagcctc ttaccgtgag ggaatcaggc
    26041 ggaggaagat accagctgat agcaggtgaa agacggctca gggccgcacg gctggccggg
    26101 ctcacacatg tccctgccta tatcaggaca gctgatgacc aggccatgct ggaacttgca
    26161 cttgttgaaa acatacagcg tgaagatctc gatgcaattg aggttgcaat aagttttcag
    26221 cgacttattg aagaatgccg gcttacccag gaacagctga gtgacagggt aggaaagcag
    26281 cgttcaacaa ttgccaatta cctgaggctc ctccgacttc ctgcagaaat ccagcttggt
    26341 ataaggaaca agcacctgat gatggggcat gcccgtactc ttgtgaatat tgaaaacccc
    26401 aaaatccaga tggaggttta ctaccatgtg gtgaatgatg atctttcagt gaggcagaca
    26461 gaggagcttg tcagacaact ccagtccgaa aagatcaggg accccctgaa agatgaaagg
    26521 aggaaaaagc ttaatgaaga gtttaagcag ctttcggaac atctgaaccg tatcttttcc
    26581 acaaaggtaa actttaaaat aaatgaaagc ggtaagggaa gaatcgtcat acccttcgac
    26641 aaccccgaag agatggagcg cattttggga atctttgacc gtcttaacta ttgaaaataa
    26701 ttatatttgt tgctccggtt cacgggtgtg taaataaaca ctgttgaata atttgagagc
    26761 aaccctgaaa atatccctga tattcatcgt gctgcaaagt tgcctttatt gcaatttacc
    26821 cgcccaggat acagttaatg tgattccggc aatcaccaga aaacgccagt tcaatccggt
    26881 tccccttaag gcaacaatgc ttgcagtggc tttccccggc cttggccagg tatacaacag
    26941 aaagtactgg aaaatcccgc ttgtatatgc aggattcggt gccctggcct acagtatcag
    27001 tttttacaca acacattaca acgatatgat gaaagcatat caggacttca ttgataaagt
    27061 gcctgaaaca gccagctaca caagatttgt gagaattgat ccggaagaat atgatcccgt
    27121 gctccatccc gaaacataca aaccttcaac ggcatcgtgg gttgaggacg gattgctgtc
    27181 agcggtggat gcctacaaaa aataccgcga tctttcatac ataggcgttg cggcctggta
    27241 cctgctgtcg atcctcgatg caaatgtaga tgccagctta ttcaatttcg atattagcag
    27301 gaacctcgat ctggccctgt atcctgtcat gatgcccgcc gccggaacat cagtggcagg
    27361 tatcaatctt aatctgtcat acaaatttta aatgacatgt tgctgatgaa cccgaataat
    27421 tgatattaaa atatgacacg gaatgagaaa tgatcaaaaa cgcgtttatt agcaccatct
    27481 cattaaaatc aaaataagat catgatcaaa cttctgaaaa cactgtttct ttccctggct
    27541 atatcaactt atgcatttag tgcttattca gtaaatgata caataattct cagatccgaa
    27601 atttacgccg agaaaattgc cacggatctt gacagcctcg tgaactcatg gtatgtaaaa
    27661 atggcattga aggagctaga accggatttc caggcagatt cgctcggaca ggaatttccg
    27721 gattccgttt acatagagag gctgaacaag ataaattcag ttatccggat accatacaac
    27781 agcattatca gaaaccatat tcatgtatat accataaggc aaagaaaaaa tttcagtgcc
    27841 gttctgggtc tgctcgacta ttatttcccg atgtttgagg atatattcga ttcaatgggc
    27901 cttcccactg agcttaagta tgtttcggta attgaatccg cccttaatcc aaatgctgtt
    27961 aacaggcata ccggagctac cggtttatgg cagtttatgt tcagcacggg aagattatac
    28021 ggactgacca tcaattctgt tgttgacgaa cggagggatc ctgtcagggc aacctacgcg
    28081 gcagccagat acctgaagga tctttatggt atatataacg actggctgct tgtaattgct
    28141 gcctacaact gcggtcctgg taatgttaac aaggcgattc gccgctccgg caacaaaaag
    28201 gattactggg aaatatatta caggttaccc agggaaaccc ggggatttat cccccagtat
    28261 gtggcagcat tatatgccgt gaacttttac agggaacaca agatcaggcc tcttccgctt
    28321 aatattccgg tttcgacgga taccatcctg gtacggaagg atattcatct caagcagata
    28381 tcggaagtaa tgggaatacc cctggatgaa ctcagggctt tgaatcccca gtaccggacc
    28441 ggtcttattc cgggctcaac aaaacctttt tcactgacgc tcccaatgag ccatttggga
    28501 gatttcatcg acctgaatga cacgattaga aaacataagc ccgaggtgta tcttacaaag
    28561 gtaaacagaa ctgaagatcc ttcacgctca tcctatgtcc ccgcggatgt taagggcaag
    28621 accaaactga tctataccgt gaaggaggga gacaatctcg gcttcatcgc tgaatggtac
    28681 aaggtgccct tatcagatct gcgatactgg aataacatat acagaaatac aatacatata
    28741 ggccagaaga ttgccgtgta cgtggatcct tcaaaagctg agtatttttc acgggtcaac
    28801 tcactgtcgt ttgcggaaaa gcagcaaatg accggaaaac cggcccctgc aagccctgtt
    28861 gcaactcctg tcctgcaggg ttcggatgat gattacataa tttacacggt ccgccagggc
    28921 gacacaatct gggatatcat caaacgatat gataatgtaa gccccacaga tgtgctgtca
    28981 ttgaacaata tttcagatcc gggcaaaata caggttggac aggtactgag aattaagaag
    29041 aagagctgac cccttatata gaaaatgcgt cctttccgat cattttcatt attactgatc
    29101 tttctgatct gtttcacagg tctgtctttt ctttttcctg atactctcaa tctccccggg
    29161 cccgaaagcc ttttaccgga agagatcata aaaaaaatcc ttcggagcga ttctgtggat
    29221 gttgctttcc ggcactctga cattcccaac cacgatactg ttcctgccgg tgactctgat
    29281 agcgattttg tcgtcatccc cgctgaacgg ccatcatccg gcccgctgaa ggcgttcctt
    29341 gattccctgc aggatccggc gagccaggta cgtattatgt attttggtga ctcacagatt
    29401 gagggggaca ggctgacatc atatctccgg tctgatctca ggaaaggccg ttcgggaacc
    29461 ggccccggcc tttttcttcc cctgatgcca gtttcgtaca caagatcagt ctttgtcagg
    29521 gcctcatcaa actggaagag atacaattac ttgtcataca aaaaccgtga gatcagccat
    29581 aatggttttg gccccctgat gtccttctgc agatatatgc cagaaaatgc caggcagata
    29641 aatccggtcg gagcctgggt aaggattgtt ccttcgaaac tggccgattc cacaacttcc
    29701 aggtatgact ttctgaaaat actttacagg aacacccagg gtgatgtaaa aattgaagta
    29761 agggggaaca aaatcctgct tgcaactgat acattgaaaa caggaactga aatcagggaa
    29821 tttgtatgtc cgctttataa ctcgagagat attctcatag attttaacgg caggaccagc
    29881 cccgatattt atggaataag tattgaaagc aaaaccggga tcattgttga taatatcccg
    29941 caaagaggca gtgcagggct tgagtttacg atgattgaca gcaataacct gagggaatcc
    30001 cttaaattat tgtctcccga tctgattatt ttacactatg gtcttaatat tgttaagaac
    30061 atcagggacg actatttgta ttacgagaaa ggtttattcc ggcagatctc aaggctgaaa
    30121 agtgttcttc ccggcgtacc cgtggtagtg gttggtgtta ccgatatggc atttggatcg
    30181 ggtgaaacag tgcgctccta tccaaatctc cccgatatta ttgttgcaca aaaaaaagca
    30241 tccgaagatg caggtgctat tttctgggat gcccgggagg caatgggagg aaatgcttcc
    30301 attatcaaat ggttcagaat ggaaccaccc cttgcaaaga aggacatggt tcattttacc
    30361 gatattggaa ccgataccct tgcaaggatg atgatatcgg atctttttac tgtcccggaa
    30421 agggaggtat ttacagcttc agccccggaa tttatttccg actcactggt gcagccggag
    30481 gttcgggatg agctgattca cgatcctgca cagcctgaaa ggagtttgtt tagaactgta
    30541 attgcctctg ttctgactta taatcctgag aagccatttg ttttttccgg cccgggattc
    30601 tgggtattct tcctggtaat acttggcgga tatggcctgg tttacagaag aatgaatacc
    30661 aggaacctct accttttcct tattagtctt tatttctatt ttaaaacggg gggattattt
    30721 ctcattctcc ttctgcttgt aattctgata gattattcct gcgggatcct tattttcaac
    30781 tcccggacaa gatcaaaaag gaaattcttt ctccttgtta gtattctttc aaacattggc
    30841 atactggcct attttaagta ttgtggtttt ttgatccatt ctttcaatga tatatttggc
    30901 acagcttttc aggtaaagga ttttcttgct gatttttcca acaggctcat gggtacccgc
    30961 ttcgatgttg gaaatattat tctgccggtc ggaatctctt tctttacctt ccagtcgttg
    31021 agttatacaa ttgatgtcta caggggcaaa ctggaaccgg taagaagtat aattgacttc
    31081 ggattctatg tttcgttttt cccccagttg gtggccggcc ccatagtaag ggcttctgaa
    31141 tttattccgc agctaagaac cgaattccat cttgacaaga gggagttcag tcatgccttg
    31201 ttcctggtaa taaaaggtct ggtaaaaaag ataattattt ctgactttat tgccgtaaat
    31261 ttcgttgatc gtgtttttga catacccact gcttattccg gatttgagaa tctgcttgca
    31321 atctatggtt atggattaca gatatattgt gatttctcag ggtatactga tattgcaatc
    31381 ggacttgcac tgatcctggg tttcaggctg ccggtgaact tcaattcacc ttacaaggct
    31441 catgggattg caaatttctg gaaaagatgg catatttccc tgtcaaggtg gcttaaggat
    31501 tacctttata taccgttagg cggtaacagg aaaggcaaag tcaggacaaa ttttaacctc
    31561 atgcttacta tgttactggg tggcctgtgg catggcgcgg atatcaggtt tataatctgg
    31621 ggaggattgc atgggcttgg tctcattgtt gacaaatcgc tggctgccgt tttcggtagc
    31681 agaacgggtt caggcagaat agttaaggct ttctcgatct ttctgacctt tcagtttgta
    31741 agcttctgct ggatattttt cagagctacc gatatgagcc acgcaggtat aatgctgaat
    31801 cagatttttc acaatttcat gccttcatcc tttggaaata tttttgaggt gtacggaacc
    31861 gtgttctctc tcattgccgt cggatatctg attcatttcc ttcctgaaac gcttaaggaa
    31921 tcatacaggg ggattttcat aaggatccct gttgcagtac agatggtcat gatcctggca
    31981 gcaggcatct tgttgtatca catgagagcc tatgatgttc taccctttat atatttcagg
    32041 ttctgagtta catgtataag gcttactcag gctgaaaccg gatgtgtcag cctgaattcc
    32101 cgggggtaag actgataatt caggcagact gaaagttccg ttagtcagga ttcagggtta
    32161 atgcacgggt gaaaggggca attgctcttt catagatccc ctggacataa gcgattgcca
    32221 ttccgtaatt tgtaacaggt acttctgcct ttattgcagg ctgaagccgg ttatgcaact
    32281 gccggcgcgt tatcatgcag cctccgcatt gtatgatgag ggcataatca ctgacagggc
    32341 gtggaagtac atcgaggcct gccacaattt catattcaat atgtttgccg gtgaatttgc
    32401 tgatccatcc aggtattttc gtccttccta tgtcatcaca ggcaacgtgg tgagtgcacg
    32461 attccaggag caatacccgg tccccgtcgc gaagcgttga aattttcggc gttcctttca
    32521 ggtaggttga aaaatcacct ttgaacctgg ccagcatgat actgaagctg gtgaggggga
    32581 tatcgtgtgg gatcagggca tcggcccttg cgaaaacctg tgaatcggtt attgcaaggg
    32641 cggggcgcag accgctcttt tccaggaagg tgcctgcctc attctccttt aaaacaacag
    32701 ctacggcatc attgtcaagt atgtccctga tggcctgaac ctgcggcaga atgagacgtc
    32761 cggccggcgc cgagacatcg attggggtaa taaggataac tatatcacca gggttaacca
    32821 gatctcccag aaggccgggg attttatatg aatgctccgg aatgctcctc ctgatcaggc
    32881 tgatgagttc ttcggtatta taattgtcaa cagcagagaa ttcaataagc tccatgccag
    32941 tgattctccg aacctcctca cggaattccc cggttggctt catgaggtca gatttatttt
    33001 ggacaataat atatggaaca tcctgactcc ggaatttctc caccaggtaa tcttcaggct
    33061 ttccccactg gttttctgca acgaccagaa gcgcaagatc gattaaatca agtgttctga
    33121 tggttcgctc aaccctcttc cggcccagtt cgcctgagtc gtcgatgccg gcggtatcta
    33181 caagaattac agggccgaat ccggttattt cgaatgattt tctgacagga tcggtggtgg
    33241 taccggcctg ttcggataca atcgcgatat cctgtcctgc aagcttgttt ataaggctgc
    33301 tctttccatg gttacggcgg ccatagatcc cgatatgcgg ttttgtatcc ctcccttttg
    33361 ccatttgttt agaatatcct gcaaaaataa caataattca tcttaatcgg ggattacgga
    33421 aactctgcat tttcatgagg gatatggctt caggctttga gtcaatatcc cggacggtca
    33481 ggttcctgat aaatccgggt gaatcaattc cggataatgg cagtccggcg gctcattggc
    33541 aacaggctgc catgctataa ctgtcaggtt atccggaatt acaatatatt catgcctgat
    33601 accggccggg cataggcaat tacatcattg cctgtaattt catccctgca aaggatgatc
    33661 agcctttcga ggacattcct gaactcgcgt atattgcctg tccattggat tttctggagt
    33721 tcactgattg catcttttct gattattttc ctgctcatgc cgtactcccc gcagatcatt
    33781 gaattgaaat gttctgccag gagcggtata tcttcggttc gctcgttaag tgttggtacg
    33841 tggatgagga tgacgctcag cctgtggtaa agatcttccc tgaaggtatt gtccgcgatt
    33901 tcctttctta tgttcttgtt ggttgctgca atgacccgga cgtttacatg cacatcctta
    33961 tcggaaccga cccggctgat cttattttcc tggagagccc tcagcacttt tgactgggcc
    34021 ggcaggctca tatcgccaat ctcatcgaga aaaagggttc ctccgtttgc ctgttcaaat
    34081 ttgcctatac gctgcttaat tgccgaggta aacgatcctt tttcatgtcc gaacagttca
    34141 ctttcaataa gttctgaagg tattgcggca caatttactt cgacgaaagg ccctttggcc
    34201 ctgttgctct tttcatgtat ctgatgggct acaagttcct tgccggttcc gttggcaccg
    34261 gttatcagta ccctggcttc cgtgggagcc accctttcga tcatttcctt aactttcaga
    34321 attgctggtg attctcccac gatctcgaac attttgctga ccttgctttt caggacctgg
    34381 gtctgggaaa taagagtcgt tttttcagtg gcatttctta ttgtaataag cagcctgttc
    34441 agatcgaggg gcttttccag aaagtcatag gccccctttt ttattgcctc aacggcagta
    34501 tcgatattgc catgtcccga tatcataatg actgggacgt cggaagagtt ctcgctgatc
    34561 ttctgaagaa cctcgatacc atccatctta gccatcttaa tgtcgcaaag aacaatatcg
    34621 tatgaatttg aattgaagaa ttcaagaccg gaaggaccgt cttcagccag atcaacttca
    34681 tgattctcat attcaaggac ttctttcagg gtgttcctta tggccttttc atcatctatt
    34741 accagaattt ttgacatatc cgtattaagt tagccggata acaccggccc ggattcccgg
    34801 aaccggctat accgttgtgg ttactgttgt ttagccctgt cttttacagt gttaaatttt
    34861 tcacaaatat tatcccgaat atctcagcca tttccacatt tctttccatg aggttttttt
    34921 accgtacatg agtattcctg ttctgtatat ctttgcagcc atccagacaa aggcagcaaa
    34981 tgtaacaacc atgagtccca tgctcaaagc aagctgccag tacggaactc cgaatgggat
    35041 cctggcaatc attacaatcg gagaagtaag gggaattatg gaacaccaca aggaaagctg
    35101 gctctcggga ttctgcattg tgcccatcgc caccataagt cccaggatta tcgggattgt
    35161 tacgggcagc atgaattgct gggtttccgt ctcattgtca accgccgatc cgattgcggc
    35221 aaaaattgag gcataaagca ggtatccggt tataaaataa aaaatgaagc tgattattat
    35281 caatccccat ggctggttca tggcagtctc gaacagcttc ccgaattcag aaatcctagt
    35341 atccgtttca ggaatactgg ttgctgtttg cacccgggtt tcttctgcca tcaggcttct
    35401 tgcggcatcc cgggaaagtt cctgcaggtc ggatttctga agtacagtcg atttaaggct
    35461 tccagcaatg gcgatagtaa ggaataccca gatcagaaac tgggtaaggc ctaccagtgc
    35521 aatgccaata atttttccca tcataagctg taccggtttc actgaagaaa caataacctc
    35581 gaccaccctg ctggtttttt cttcgataac gccccgcatt acctgcgccc cgaacatgaa
    35641 aacaagcata tacataagaa aaccaccgat atacgcaagg gccatggcta ccccggtact
    35701 ggtttcctta acattgcctg aatcatcgat ttttattgtc tggatcgata ccctggtttc
    35761 gacatttttc atgatctcat cgagattctc aatatcgtat gccttcagtt tctgtttttc
    35821 aatctctttc tccagggatt tttcgatatg ttccagcatc ccgatcggag gctgtttttt
    35881 tgaaagcagc tgaatcgcat tgggcgtagt gataatttcc ggtgagatgt acagaatacc
    35941 gtaatagccg gcctgttcaa atgaattttt aagatcgctg atcctgatgc cttcaagata
    36001 tacaaactca agattctttg tattgcctat tttccccctg aaaagatcgg taccatcctc
    36061 aataactgca atctttttgt aatcctgttc ctggttcatc atcagcagtg tggggacaac
    36121 tatcagaaga gccattagca aaggggccag gattgtcatg ataatgaatg attttttcct
    36181 gactcttgtc aggtattccc ttttgattat tacggatacc ttgttcatca ttattggatt
    36241 tttacatctg gatgactaat tcctggattc caccaccctg atgaagattt cattcattga
    36301 gggcagggcc ggattgaatg agattatcct gcccgacacc agtgcattcc caagtacatc
    36361 attggtgtca atcccgtccc taatcctgaa tctgatcctg gttcttccat tttcatcatg
    36421 ccttttgaga atattgattt tttcctgttc aggaagaacc gcattaccct caaatactat
    36481 ctcatattcg ttggctgccc attgtctcct tatgtcgtca acgtttccct ccagtatggt
    36541 ctttgccttg ttgatcaggg taatattatc gcataattcc tcaactgact ccatgttgtg
    36601 cgtcgaaaag atgatggttg accccatttc cctgagaaag aggatttcgt tcttgataat
    36661 atttgtattt acaggatcga agccggtgaa cggttcatcg aaaattatca gcttaggttc
    36721 atgcagcacg gtcgtaatga actgtacttt ctgctgcatg ccttttgaca gctcttctac
    36781 ctttttaccc caccactcgg ctatatcaag cttattaaac cagtatctga gccttttcat
    36841 ggcttcagct tttgtaaggc ccttaagctg ggcaaagtac aaggcctgtt ctccaacttt
    36901 catttttttg taaaggcctc tttcttcggg caggtatccg attctgagga cgtcatcggg
    36961 tttaagcttc tttccttcaa ggaacagctc gccggaatca ggtccggtta tctggtttat
    37021 tattctgata agggttgtct ttccggcgcc gtttggaccg agaagaccaa aaatgcattt
    37081 ttcggggaca gaaatactta cattgtcaag ggccagctgg gtaccatact gttttgtaac
    37141 atttttagct tcaaaaagtg ccatagaatt tcacagattg ggcaaaagtg atgtaaatat
    37201 aagcaactct gtgaaatatc cggaatgata ttcggtatct attcaaaata tcctctcatc
    37261 cgttcgaaga atcccttgtc ttctttatcg ggcctgggta tgaaggatgc tgaatccctg
    37321 agtttttcaa gagttcttgc ttcttctttt gtcagattct tgggaatcca tacgtttacg
    37381 tttacaagca agtcgcctct gccgtatccg ttaacttcag gaaggccttt tcccctgagc
    37441 cgtagtatct tgccaggctg ggtgccgggt tctatcttta tttttactat gttctcaaca
    37501 gttggtattt caacatgagt accaagggca gcatcaggga tgctaatgaa aaggttgtag
    37561 ataagatctt ttccctccct tataagttcg ggatgatcct cctcgtcgat aacgacaagc
    37621 agatcgccat taattcctcc gcgacgggcg gcatttccct ttccgctgac tgtcatctgc
    37681 atacccttac tgacgcctgc cggaatattt atcttaatga tatcatcttt ttgaactatc
    37741 ccttcgccat agcatacagg acatttcttt gtgattgttt ttccgtttcc tccgcacgaa
    37801 ggacatactg atgtggtctg catctgaccg agcatcgtat tggtgatcct ggtcacatgg
    37861 cctgaaccgc ggcatgtggt gcatgatgta atactgttct gatctgccgc gcctgttccg
    37921 tcacaggtac tgcagacaac atatttggcc accttgatct tcttttcaca gccgtttgca
    37981 atttcgccca gggtcagttt taccttaacc ctcagattgc ttcccttgtt cacccttctg
    38041 ctgcttcgcc gggagcttcc aaatccgccg aagccgccaa aagcatcgcc aaaaatatca
    38101 ccgaaagaag caaaaatatc ttccatggtc attcctgccc ctccgaatcc gttcccggaa
    38161 gcaccgctca ccccttcatg tccgaacctg tcgtaacgcg cccgtttttc atcgttgcta
    38221 agtacttcat aggcggaggc agcttcctta aaatgttcct ctgccttttt gtcgcccgga
    38281 tttttgtcag gatggtattt caatgcctgc ttccggtagg cttttttaat ctcctccttc
    38341 gtggcttccc gcgaaacccc aagcacttca taataatctc tcttgtccat cccttaatat
    38401 ttggaactgt tttatcaatt tactgtatgt gatttgaagg cttattcgcc cactaccact
    38461 ttagtatgtc ttaccacctt gtcgtggaga aaataaccct tgaggatcac ttccacaact
    38521 tttcccctgc tgtcctctcc aggcgccggt gctttggcaa cagcctcgtg aagatcaaaa
    38581 ttgaaatcct tgtttaacgc ctcaatctcc ctgataccct gctgtttcag gaaatcagag
    38641 aattttgagt aaatcaggtc aagcccgtct ttcagactct gacagtccgg agagacttcc
    38701 atatgcttaa gagctctttc aaaatcatcc attaccggga gaagtttcac cagcagatct
    38761 tcgcctgcat attttgtaat ttccatcttc tcacgcaggg tgcgtttcct gtaattatcg
    38821 aactcggcgg cgatacggat atacctgtcc tgcatctctg ccagtttttc ctcaaatgac
    38881 ctgccttcag ctacaacttt gtcaggagtg tcctgtaaag tttcacctgc aaactgattc
    38941 tgacccatgc cggctccgtc aattaacgtt ttgtcctcct cctgttcatt tacgatgggt
    39001 atctccttat tgtctttact atcagtatac ttatcttgcc ctgtttcttt gccgtgcacc
    39061 gtttcagaca tttttttcct taccattact tgattgtttt ctgataatta cacttgcgtt
    39121 catcaaaatt tctgccaaca cccgggattt gacaaattgg caggaaaaat cccgatttcc
    39181 gtcaactgga ttaaattatc cttaggtaga agatgataaa tcagaatata taagccggga
    39241 atgggacagt ttaaatggga tttgcagttc aggactgctt tctggagtta aattcttttt
    39301 atatctgctc caatggcgtt aaggcggtca tcaatattct gatagccgcg gtcgatctgc
    39361 tcaatattat gaattatact cctgccctca gccgacattg cagcgatgag cagggccact
    39421 cctgccctta tgtcgggaga ggacattgtt gtacccctga gtttcatcat tttatcaagc
    39481 ccgatgaccg ttgccctgtg aggatcgcat aaaatgatct gggcacccat gtcaataagc
    39541 ttatcaacga agaaaagccg gctttcaaac atcttctggt gaataagaac ggctcccttg
    39601 gcctgggtgg cagtgacaag aaaaatgctt agcagatcgg gagtaaggcc gggccatatt
    39661 gcatctgcaa tcgtcataat tgatccgtca atgaaagtct ctatttcata atgttcctgg
    39721 tatggaataa agatatcgtc cgagattctt tcaattctta ttcccagccg ccggaaggaa
    39781 tccgggataa tccccaggtt atcgtatgag acatttttta ttgtcagttc cgatccagtc
    39841 attgcagcaa gccctatgaa tgaaccgatt tcgatcatgt cgggaaggat cttgtgggaa
    39901 caacccccga gctgccttac gccgtcgata taaagcagat ttgagcctat tccgctgatt
    39961 cttgcgccca ttgagaccag catcctgcat aactgctgga tatagggctc acaggcggcg
    40021 ttatacagtg tggtgcgccc ttttgcaaga acagaagcca ttattatatt ggcagtaccg
    40081 gtaacggatg cttcatctag gtgaatatat gatcccttca gaccggaggc ttcaacgctg
    40141 aaaaaaggat cagccgtatc aaattcaaat tttgctccaa gcttctggaa acctgtgaaa
    40201 tgggtatcca ctcttcttct gccgattttg tccccacccg gtttgggtat ataggccttg
    40261 ccgaacctgg caagaagggg tccgactatc atgatggatc cccggatact ggcactttga
    40321 tttctgaatt cctgtgactg aaggtaatcg agattaatgc tgacagcctc gaaagatgca
    40381 gtgccgcgtg acttcctggt caccctgaca ccaagtgcca tcagaagctc aatgagtttg
    40441 ttcacatcat ttatatcagg gatgttcctg atcgtgatcc tttcggatgt cagcagaacg
    40501 gcacaaataa cctgcagagc ctcattcttg gcaccctggg gatgaatatc cccgctgagt
    40561 gttttgccac cctgaacaat aaagctgccc attaatgacg attgtgacct tttttcttcg
    40621 ggaattgctt gttttgctgt tttccgtgag gtttgccctg ctggggtttc ttttttgcag
    40681 acggaacaag ttcctttact tcaagcagtt tgattccttc tttcagtttc agctttcctc
    40741 cagaaaggaa cttcatatcg cttattatga cctcatcgct tacctgtgcc cggttccagg
    40801 taacatatga ttttttcatc tggttgagga tgagatttgt gatatactct ttttcttcac
    40861 cctcctccat ttcagcagca gcatcgatca tgagctcaat gatccggcca taatgcagaa
    40921 acctgatatc cccctgctta taaggaatct gtttcggttt ttcctcgaat ttgaattttt
    40981 cgggaggagg atacggggaa tctatgtcga gctcaaaatc agcgatcagc gcaagatgat
    41041 cccacaactt gtgtttgaaa tcagtcacat cacgtatctg gggattaaga ttacccatta
    41101 tgtggatgat ggtctttgca gcacgatttc tctcttccct gtcagaaatg gttttgatgt
    41161 gatcgatcat tttctgaaca tttcttccat attcgggcaa tgccatccgt ttgcgctggg
    41221 tattgtagtc gtaattcatc tgctgttaac tgttttctgc aaagctaata attaattgcc
    41281 gaataatggc aaggagatca gagggtctag agcccaatga actggattgc gataccagac
    41341 aggaaaatca tgcctccggc aataagattg ctgtattttt cgaccggctt aagattgatc
    41401 ctgctgaaac cgagtttgaa ggcaagcacc acacccatca tggttgcaat tgtcaccagt
    41461 gaaaacagca cggaaacaaa aactgaaccc gctatattat tttgggctgc cggatacatc
    41521 aatatgggaa taagtggttc acagggcccg aaaacgaata tcaggaacag tatccacgga
    41581 gtggttctga caacgtcatt ttcgtgaaca tgtgcatgat ccccgaaatg gttgtgttca
    41641 tgaatatgct ccccggtacc cggatgaaaa tgtgaatgtg tatgctgcct tttcctgtaa
    41701 agattcctga tgcttatcag catatatatc aggccaaatg caataaagag ccatccggca
    41761 atgttgcctc tgaatgattc cacttccacc agcctgttga ctgatattcc cacaataatg
    41821 cccgtgagcc caaggataac tgaactaaga acatgaccaa gcccgcaaag gaaagtgatc
    41881 cacatggttt ttttcatggt ccatttcttc gcctcgctca ggacaatgaa aggaagataa
    41941 tggtcaggtc ccagtacagt atgaatgaat ccaatggaaa ctgccgtaac cgaaagaagt
    42001 gtgattgaat tatccattat ctgaactttt aggattagaa gtaattattc ctgcaataat
    42061 acccataaaa tcaggattgt cgtgatgcag caggtggaat gacgcatttt gcagatgttt
    42121 gtttcagcga ttccattcat taccggtaaa aatatactcc ctgaaattga tattctgaac
    42181 ctcaaggaac ctacgggagt ttgtgacata gtccatcatt ttgatatggt cggagggact
    42241 ggcaaagaat tcactgttcc tttcatattt cgaacaggga agtatataga accttttcct
    42301 cccgttttca aagggagtgt aaacccatgt caggtaataa ccggcactgg ctattttcag
    42361 ggaatcattt cttttttcaa tttcgattct caccatggca cctccgtcgg tttttggtct
    42421 ttgctggttt gaaacgaaat tgccgagaga atagacggcg atccggccct taccgtatgg
    42481 atcctccttg gtccagatca ttttctgcag aacatgcgga tgtgatccta ttataaggtc
    42541 tgcgcccctg tcgaaaaaaa aacgggccat ctcatcctgc atcttcgatg gtatcgtgtc
    42601 atattccgtt ccccagtgga gaaaaaggat aatcagatcg ggattcttcc tcactgcttt
    42661 ttcgagatcc tccgctacaa gatcccggtt cagcatgttg acgataaccg gatccgggac
    42721 atttatcccg tttgtaccat atgtgtagtt cagcagtgca atggtattgt ttttaatatt
    42781 gagcataagc ggataaaggg aatccctttc gtttttactg gcaaaggttc ctgtatgtgg
    42841 tattccaagg ctgtccagct tttgcagggt ttccagaatg ccttttttac cgcggtctgc
    42901 cgagtgattg ttggcagtaa caaaacaatc tattccggcc ttgatgcagg ctgcggcgag
    42961 ttctgcaggg gagctgaagg ccggatagcc cttgtaagga gatcctgcca gggtaacttc
    43021 aaggttagca atggcatagt ctgcttccga tatttcttcc ctgataaagc tgaatacatc
    43081 atcataatta aaggatccgg tctccgggtc tttggcagac cttatctgtg catcatgacc
    43141 cattatatcg ccaatgaaaa gaagtgatag tctgtctgtc aggctgtcag caggattttg
    43201 tgcaggagac attccaactg cgaggacggc cagtatcgga attaatattt tatgattcat
    43261 aaaatataat tgagcaaaga atccgtaaat ttaataaata ttaaatttgc caccttaaaa
    43321 tacctgaacc ctgtgtggtc tctgctccgt agtaatgcct aaagctggaa acaatattga
    43381 cataaacaaa atttctgcgg ccggtctgct ggttacgctt ggtattgtgt tcggcaacat
    43441 cgggacaagt ccattatata ccatgagtgc tgttctgaac gggggtaaag gcaatttcgg
    43501 tgatcttctt gtttatggtt ccctgtcatg catattctgg accctcacct taacgaccac
    43561 catcaagcat attgcgatta ccctgcgggc cgacaacaac ggagagggag gcatatttgc
    43621 cctttttgcc ctgatgaaaa acaaatcggt ttgggttgca gtgcttacaa tgattggcgg
    43681 aggagctctg cttgcagacg ccataataac tccttcaatc acggtaacct ctgccattga
    43741 ggggctggaa ctgtttaatc cgaaaattcc cgtggtcccc attgttttag tcataatagc
    43801 cgctcttttt tttattcaac agttcgggac acatttcata gggagctatt tcggtccggt
    43861 aatacttgtt tggttcctga tgctgggatt gctgggtctg tcccagataa tttcaaatcc
    43921 tctgatcctg gggtcgctta atccggttta tgcctatcgt tttttaagcg aatttcccgg
    43981 tggctttgtt cttctcggag ccgtgtttct ttgcaccacc ggagttgaga ccatttatgt
    44041 tgatctcggg cattgcggaa gaaataatat cagagtatca tggatatttg taaagatcag
    44101 cctgatgctg aattatatgg gtcagggagc atggctgctg caaaactata aaggcgggga
    44161 agacctgaat ccgttctttg aaataatgcc ggaatggctg ttacttcccg gtatcgttat
    44221 tgccacaacg gctgctatta ttgccagcca gtcgattatc agcggctcat tcaccctcgt
    44281 cagtgaagcc gccctactca atttctggcc aagactgagg gtcctccatc cgacactcgt
    44341 gagaggccag gtttacatgc catttgttaa catgatactc tggattgcct gcaccatcgt
    44401 ggtcctgatc ttcaagaagt caggcaacat ggatgcagct tatgttcttg ccataacaat
    44461 aacggaaatt gcaaccaccc tccttctggc agtttatctg tttcaaaaag gcgtcaatca
    44521 caggttaata tcctttatgg taatggtcta ccttacagtt gagggcagct ttcttatagc
    44581 caatcttttc aagttcagca aggggggctg gttcacgctt atcctcggcc ttctgtttat
    44641 ccttatcatg ctgggatggt attttggcag aaagatcaaa aacaggtata tttcttttgc
    44701 aaacctcaat aaatataccg atctgttcag ggatctgtca caagatgaat cagtacccaa
    44761 aacagcaacc aaccttgtgt atattatccg tgcaaacagg aaggatcagg tcgaatccaa
    44821 ggtcgtctat tcgattttca gcaaatttcc caaaagagcc gataaatact ggctgcttca
    44881 tattgatcgg gttaatgagc cgaaccagtt tgagtatcag gtaacgcaaa taattccggg
    44941 aattctgatc agggtggatt ttcatatcgg attcaaggtc gaacccagta taaatcttta
    45001 tttcagggaa gttctggaag atctttccgc gaccggagag ataaagcttg aaagcgggta
    45061 tgaatcactg aggaaatatg acataccccc cgatttcaaa tttatcctta ttgaaaggat
    45121 tatgccccgt gattacaagc tgtcaaactg ggagaatttt attctttttc tgaacaatat
    45181 tgccggcaaa atgagtattt ccgacattaa ggcccttcag cttgatgcaa caaataccgt
    45241 gattgaacat gtacccataa ttattgacca gccttctcac cggaggatac aacgca
//
LOCUS       DAOOYG010000007        42548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_758_length_42548_cov_7.805309, whole genome shotgun sequence.
ACCESSION   DAOOYG010000007 DAOOYG010000000
VERSION     DAOOYG010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 42548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42548
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_758_length_42548_cov_7.805309"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1750
                     /locus_tag="PK288_01440"
     CDS             173..1750
                     /locus_tag="PK288_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPM17607.1"
                     /translation="MKKIISLAVLLVLLGACTKNFEEINSNPYQITDESLTQDFNHVG
                     AYFQGLLSNLQGHQVEEDLATDSFVRHFGTPTPFVSNRNNTTFYITWNRFWGRIYNSV
                     MSPSRQVIKIAEEGGYDVFAAWAKFIQVMGLSRLTAYHGPLIYSEYGKPTTPILYDSE
                     KTLYDSFFRVIDETQAVFAANKTYPGLKKFDASYGGDITKWIKLMNSMRLRLAIRLSK
                     ADPTLAKQQGEKAIADAGGLILTNADNFNISLYGAKMPLAVICFEWQDTRMGAGMEEF
                     LVGLKDPRIGKYFAPAKDASLYTDHPAFPYKGIASGAYLGAKDDRQLYSEVSEDWKSV
                     TSRRFFTAAEVHFALAEAALRGWAGAGDAKTHYEEGIKMSFQNWGAGGVDTYLADATS
                     TPINYVDPKDSRNSYTTRSTVTVAWDETATKEEKLEKIITQKWIDALTNANEVWCDHR
                     RTGYPKLHYVPKNDSNQDWGIIPADGFLQRMPFVDAERNSNPEGVADATTKLGGPDLI
                     STRLYFAPAGPNFPDEP"
     gene            2201..3682
                     /locus_tag="PK288_01445"
     CDS             2201..3682
                     /locus_tag="PK288_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HPM17608.1"
                     /translation="MKKNLMFLLVLAISTLTVMAQLDLKKTGTQIKKQAGQLSNEKKP
                     AEAPASGGASQAGTSTATSGPSKTLYVSSTGSNRNDGSREKPFKDIQKAIDEAPDGAV
                     ICVTQGNYLGKLDAGYISIKKYISLVGGYSEDFSERDPVRYRTTIQPGPEAGGTNANF
                     GLIDIDIRTNPKGRVLIDGFILDKGQMNRYVGLNANDARFKAPEGVETGHLNPPGKQI
                     GQPSMRGPSTVSNQLIHGYVKGDLIVRNCVFLNGSHFGIQVGNIGGHFEIYNNIFLAS
                     RMAACEIYSMNNTPGEAVLDFHHNTVLFTWRRDWVPGDKDMGYGFRYMTGINANVNNN
                     IIGCSDFSALDRTRIDSDKNREAQRKTSANNNLFFGNIEADLTLPSGGGKFMRIFARQ
                     FEDVDQLAEAEGNREMSEAEIKILSRAIDAAYLKAFLNIEGSSSSTYNPNSSENLFRS
                     AMGMNQRGTETNYVSMYANSYPFDKATDLFGALEGYGAQKIRD"
     gene            3755..4021
                     /locus_tag="PK288_01450"
     CDS             3755..4021
                     /locus_tag="PK288_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17609.1"
                     /translation="MKAFPNELDSGLSFKGNICMIGCGSIGQAFLPVIGRHLGGIYSR
                     LTVLSACESGRRVAQGRGRYFPEKLDVQSPWQLQNIRSRQYHGE"
     gene            4302..5066
                     /locus_tag="PK288_01455"
     CDS             4302..5066
                     /locus_tag="PK288_01455"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPM17610.1"
                     /translation="MRLILKNIIWLTVHGFIMNPAFSQGMTAADIVMKADEKFNGEKS
                     SYSIISMTIVRPSWQRTIDIKTWSMGRDYSLALVTAPPKEAGQSFLKRGSEMWSWIPS
                     ISRLVKLPLSMMSQGWMGSDYTNDDILRESSLVNDYLHEIAGEEIIDGRLCYKISMKA
                     RPDASIVWGRQTRWIDRKDFLVLKAELYDEDGYLVRTETASDIRIMDGRTVTTRIELV
                     PAEEPGNKTILVIRDIKFNLPLGESFFSQQNMKRVR"
     gene            5081..6499
                     /locus_tag="PK288_01460"
     CDS             5081..6499
                     /locus_tag="PK288_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPM17611.1"
                     /translation="MKEEFRLAWRNLWRNRRRTVITAASVFFAVFFTVITRSLQLGSY
                     DHMINNIIQSFTGHLQVQHIKYHDDPLIDNSFAYNDSLISAISAIEKVVSVTPHIESF
                     TLASNGPQTKGVAVIGIDPAREKHFSDPEAKLVRYRITGEAVEGMRTGELPEKVLEKV
                     EKNIGRSYSSAARIELELGLTDEEKNHCLSQILKYSEFKNGYLSPDDKGILLADKLAG
                     FLRAGIGDSIILMGQGYHGVSATGIFPVRGIIKLPSPGLDSRLVIMTIPAAQQLFDAD
                     GKITSLVINLKDRSGRTITKARNKINALLPDTNTSARTWYELNEVLYQQMRGDSQSGK
                     LMLLILYFIIFFGIFGTVLMMISERRREFGVLVAIGMRKGKLKRIVIIEMVMLGIIGL
                     ITGLLAGTPLITYFNHNPVIIKGDLGKMMEDWGYDAMMPTAPVGSYFYWQVVIVILMI
                     LLAAIYPLRKIGKLKETEALKS"
     gene            6513..7937
                     /locus_tag="PK288_01465"
     CDS             6513..7937
                     /locus_tag="PK288_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPM17612.1"
                     /translation="MIWTLAWKNIWRNKKRSLVVIFAVTLGIISGMFLIGFVEGWSRQ
                     RLHDAIYNEVSHLQIHNSEYPGNEETDLTVINPVRLVLHIDSLKELRSWAKRTRIVAM
                     ANTPWGNTGLTLFGIDPEREKLLTEIHDRIVPGGGRYIDSGNPSGILISDKTADLLRL
                     KHYILTDSVMEELRHEKIPVSVLERINQLRNVRFRSLKDFREGLMPLLTGKETARYGF
                     LISGKALNYRLRNRVQVTISDIDGNPVQGIFRVCGVFKTANTGFDQSAAFVNAAELAR
                     QYGSGEIPVHEIAILLNDIDDADMVKNTLNAVAGGNSVMTWKELAPEAALMNEFMFAY
                     YLVFIGIIMLALAFGIINTMLMAVLERTKELGMLMAIGMNRRRIFTMIMLETIFLTFV
                     GAAAGTVAGLALIAVLGKTGIHLGSWREGFEAIGFAATVYPFVTPAFIVFTVIMVTVT
                     AVISSIWPARKALKLNPVEALRTE"
     gene            8023..8712
                     /locus_tag="PK288_01470"
     CDS             8023..8712
                     /locus_tag="PK288_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM17613.1"
                     /translation="MKVLELKNVNKIYNSSEIKVHAVNNVTLDFEEAEFAAIVGPSGS
                     GKTTLLNLIGGLDMPSSGEIIVGGTILSELKPSQLIDFRLHNIGFVFQSFNLIPVLTA
                     RENVELIMVLQKWPENKRNLRTIELLNAVGLGDRLNSRPARLSGGEQQRVAVARALAS
                     KPKFVLADEPTANLDSKSAATLLEIMVNLNREENVTFIFSTHDPRVVKMARRIIILED
                     GKIVSDEKQCQ"
     gene            8693..9874
                     /locus_tag="PK288_01475"
     CDS             8693..9874
                     /locus_tag="PK288_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17614.1"
                     /translation="MKNNVNDFMSINQRIVLCLLAVFIFFPSVSQEKERKIELKGYFS
                     TLQTVIFDSLSGPFANENLLHNRLNFKAYLNDHLTIGAEFRNRLFTGDLTMLGTAYTD
                     LIGMDRGVVDMSWDLLREQSIYLNSRIDRLWIDYNYRKIQVTLGRQRINWGQTLVWNP
                     NDIFNAYSFFDFDYVEKPGSDALRLQYYPSSSSVAELAFKLDNEYDLTAAGLFRFNKF
                     GYDFQFLAGLVNSEDVVMGAGWSGSVGSLSFRGEGSWFSPYEQFPKSRGTILVTSGLE
                     KVFKNNSSAAIQLMYSNNPLGFIDFYSILGGELSSKNLAFSEFTAFGQFTWAITPMLN
                     LTVSSMWFPDLDGYYAGPSLEYSLAENVDFSVIWQHFDAVLAGVRNIINLGFLRFKYS
                     F"
     gene            9918..10655
                     /locus_tag="PK288_01480"
     CDS             9918..10655
                     /locus_tag="PK288_01480"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPM17615.1"
                     /translation="MALVHELEKSGNWLFKRRSWLPLILIAAGIVVLYHTNRQAILFN
                     PVKELIFLGVSLFGEALRVYTVGFAPRNTSGRNTSAGQIADELNTTGIYSVIRHPLYT
                     GNYFMWLGPVLFVRNPVFILFFLLVYWLYYERIMFAEEQFLRRKFGEEYDKWSENVKA
                     VIPCFRKFRKPLMAFSFRSVLRREYHSFVNIFIIFLILDIFRNYFISSRVQVTPMWLF
                     ISIAAGLIWLVLRIIAKKTSWLECDDR"
     gene            complement(10665..11345)
                     /locus_tag="PK288_01485"
     CDS             complement(10665..11345)
                     /locus_tag="PK288_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPM17616.1"
                     /translation="MNIWLIFGVFMILSWIISSVLKSKFRKYSRIPVDNGMSGREVAE
                     RMLRDNGIYDVTVGCVQGELTDHYNPDKKTINLSREVYEGRSIAAAAVAAHETGHAVQ
                     HATAYAWLGLRSKLVPPVTFASKWVQWILLGGIILVNTFPALLLTGIILFALTTLFSF
                     VTLPVEINASQRALAWLSNTGITSTMNHDKARDALKWAAYTYVVAALGSLATLLYYIM
                     IFMGSSRD"
     gene            11548..11778
                     /gene="yidD"
                     /locus_tag="PK288_01490"
                     /pseudo
     CDS             11548..11778
                     /gene="yidD"
                     /locus_tag="PK288_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
     gene            complement(11892..12644)
                     /locus_tag="PK288_01495"
     CDS             complement(11892..12644)
                     /locus_tag="PK288_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPM17617.1"
                     /translation="MEKIRAVIIDDEAPARDLIRHYLTDLEEIEVVAECSDGFAGLKT
                     IVSEKPDLVFLDIQMPRLTGIELVEVMTEKPEIIFTTAYDHFAIRAFELNAIDYLLKP
                     FSKRRFIEAVKKATLKIRAGAGNTGPASLLLSKRPDPDSPVNRIVVRKGNAISIIPVD
                     QIRYVEAQDDYVMIHHSSGKALKQHTMKFYEDNLSKKDFVRIHRSYIVKVEEIKGIEP
                     YGKDNHIAILLSGARLPVSRSGLRNLKEVLDY"
     gene            complement(12712..13779)
                     /locus_tag="PK288_01500"
     CDS             complement(12712..13779)
                     /locus_tag="PK288_01500"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPM17618.1"
                     /translation="MRHPVLSDRVRLIIWWLAWLLLTAGQLFLYYFAYGEITEIFIPD
                     GIISMIVFSGIGISLWYPFRFINAGKSALISVAGNILIGGTLSVALWIIVTRFAVPLF
                     LPVNSGFEAYWQATFPYRAGTGGLIYALIILAYYLFETLYNLSEKNAREARLETLVRD
                     TELKALRSQINPHFLFNSLNSISSLTVTNPDKAREMIIKLSEFMRYTLSKKDEQPVTL
                     RSELENLKLYLEIEKIRFGDKLSTGEEIDENCLDIKLPVMLLQPLYENAVKHGVYEST
                     EIVRINTRVRCSEGFLEIVISNNYDTVPSPVKGTGTGLLNVTRRLDLFYGSKASVRIA
                     RENGIFSVTLVLPANVEDNQK"
     gene            complement(13790..14533)
                     /locus_tag="PK288_01505"
     CDS             complement(13790..14533)
                     /locus_tag="PK288_01505"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPM17619.1"
                     /translation="METHNDFKDQEHRQLGPHRSSNRAVIGVVLILAGLFLVIRNTGF
                     FPDFIDNIIFSWQMLLIVIGLVMTLNATEKTAGIIVMAVGGFFMIPLAFRETLHMYNM
                     FWPAVFIIVGIIFVTTKRRGWGTSTSKGVTGDDYVDYVNVFSGGDRQVTSRNFQGGKI
                     TAIFGGIELDLTKADLVQGVSVLEVACVFGGATIIVPNDWFVTIDVTPVLGGFSDSRK
                     LTPGMTIDKNKQLLIKGAVVFGGGEIKSY"
     gene            complement(14558..14926)
                     /locus_tag="PK288_01510"
     CDS             complement(14558..14926)
                     /locus_tag="PK288_01510"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPM17620.1"
                     /translation="MKNKDHFILRHREHHPQVGFGLFLITLGLALLVATNDLLNLGEI
                     SDYFTWQTGMIFIGVLLILNLQFTGGLLLIAAGTWFLMDKINYEMPEIIKAIYWPAVI
                     ILIGLGFIISSLVKRKTKSI"
     gene            15116..15637
                     /locus_tag="PK288_01515"
     CDS             15116..15637
                     /locus_tag="PK288_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPM17621.1"
                     /translation="MSGLYLIPIGGLKEGYRSFEFEIDNSFFGLFEESEIKEGKLTAV
                     VETVKSPSLIEMLIRISGTVRIGCDRCLEMFDEVLNCENRLVVKFGNTADVADPEIII
                     LPYGENELDMKQFFYEFIHLALPIQRIHPDDENGNSGCNPEMLSKLREHLVNEESLVD
                     PGWDELKKLMNNN"
     gene            15652..15837
                     /gene="rpmF"
                     /locus_tag="PK288_01520"
     CDS             15652..15837
                     /gene="rpmF"
                     /locus_tag="PK288_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPM17622.1"
                     /translation="MAHPKRKHSKARRDKRRTHFKATPPTTGTCSNCGSSVQYHRVCP
                     ECGYYRGKPAVEKKATA"
     gene            15861..16799
                     /gene="plsX"
                     /locus_tag="PK288_01525"
     CDS             15861..16799
                     /gene="plsX"
                     /locus_tag="PK288_01525"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPM17623.1"
                     /translation="MRIGFDVMGGDFAPEAMIEGAVDSISYFGPDEKLVLIGDKREIL
                     KKLEEMNTAPDLFEIVHSSQVIEMGDHPAKAFSQKRDSSIAIGYEMLKQGLLDGFCSA
                     GNTGAMLVGASYTVNVIPGVFRPALATILPCIDGRDSVILDVGLNPDCKPDVLLQYGI
                     LGSIYARFVLGKNNPTVGLLNIGIEETKGTPAIKAAYELMKDYPGLNFSGNVEGNTLF
                     HESVTDVLVCDGFVGNIILKQAEAFFSISEKLKISHPFIDRMNFENIGGTPVVGINAN
                     VVIGHGISRRKAIKNMILQTKAVVGANLARKIIEAI"
     gene            16802..17800
                     /locus_tag="PK288_01530"
     CDS             16802..17800
                     /locus_tag="PK288_01530"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPM17624.1"
                     /translation="MEEVRAVITGIHAWVPEYRLTNHELSKMVDTSDEWIMQRVGIKE
                     RRILKGEGLGSSDLGEKAVKGLLEKTNTSPDEIDLVICATITPDMVFPATANIISDKA
                     GLKNAFSFDLGAACSGFLFALQTGAAYIQTGRFRKVIVIGADKMSSITDYTDRTTCPL
                     FGDAAGAVLLEPGTGDYGIMDYIFRVDGSGRKFLHMKAGGSVKPASHETVNAREHFVY
                     QEGQSVFKFAVVNMADIAVEIMERNNLKPEDIAWLVPHQANLRIIDATGKRMGLPPEK
                     VMVNIENYGNTTAATIPLCLWEYEKKLRKGDKLILAAFGGGFTWGSLYLKWAYDPD"
     gene            17967..18368
                     /locus_tag="PK288_01535"
     CDS             17967..18368
                     /locus_tag="PK288_01535"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPM17625.1"
                     /translation="MGKFNETDNTAINLISNGTEITGDIKSNGDIRVDGLLTGNLTTK
                     GKVVIGPTGKVKGEVYCKNSEVSGTVEGRICVSQLLTLKVDSRILGDIVTFKLSIEPG
                     AKFTGNCKMSESENNEGASFTKTKEPEKSVK"
     gene            18316..18561
                     /locus_tag="PK288_01540"
     CDS             18316..18561
                     /locus_tag="PK288_01540"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPM17626.1"
                     /translation="MKEPASQKQKNPKSPSNKGLHDFAKYSGLAFQMLVIIAAMTWGG
                     VKLDEVLGLSTPVFTIVLSLLGVFAGIYTAIKDFIKR"
     gene            complement(18551..18799)
                     /locus_tag="PK288_01545"
     CDS             complement(18551..18799)
                     /locus_tag="PK288_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17627.1"
                     /translation="MTDSATIRYIVPPSAPFFSPDLEISAAVMPIAENETVSNNRSVK
                     RNGTGMNRDNRYPAASRGRQTHKSPVRYFLKFICEIIV"
     gene            18970..20040
                     /gene="atpB"
                     /locus_tag="PK288_01550"
     CDS             18970..20040
                     /gene="atpB"
                     /locus_tag="PK288_01550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPM17628.1"
                     /translation="MQFVKASLLFLLFTIIPAALTAQEEHGSHAGRAEEEFDASTFIM
                     DHIADSHEWHILTRKNGESVAIYLPVILYSAEKGWDVFSSRQLAHGHEYKGYRLEEEG
                     DNKGRIVSLNEDGTINEEAFLLDLSITKTVVGLLFSVIIGLWLFLSLSRSYKKTGISH
                     PRGIQSFLEPIILFVRDDIAIPNLGEDKYEKYMSYLLTVFFFILLNNLMGLVPFPPPF
                     GANVTGNIAVTMVLALFTFFITQINGSKSHWRHVFATPGVPVWLLPVMIPVEIIGLFS
                     KPFALMVRLFANITAGHIIVLSLVCLIFIFKSLFVAPVSIAFVIFMDCLELLVAFLQT
                     YVFTLLSALFISLAVKGEEEHH"
     gene            20073..20279
                     /gene="atpE"
                     /locus_tag="PK288_01555"
     CDS             20073..20279
                     /gene="atpE"
                     /locus_tag="PK288_01555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPM17629.1"
                     /translation="MTGTLAAVGASLAVIGAGLGIGRIGGSAMDAIARQPEASSKIQT
                     NMIVSAALIEGAALFAIVVALIAK"
     gene            20351..20866
                     /gene="atpF"
                     /locus_tag="PK288_01560"
     CDS             20351..20866
                     /gene="atpF"
                     /locus_tag="PK288_01560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPM17630.1"
                     /translation="MVLLANSLTSPAIGLVFWTTIIFLMFFLILKKFAWKPILKAVKA
                     RDEMIKGSLASAEKARKEMARLQSDNEAILRKAREEREIILREARDIRDKLISEAKGK
                     ASEEAEKVVAKARAGIEREKIAALGEIRKQVATLSIEIASKLIGEKLEKSGEQEKLID
                     NYLKEIDFTKN"
     gene            20877..21419
                     /gene="atpH"
                     /locus_tag="PK288_01565"
     CDS             20877..21419
                     /gene="atpH"
                     /locus_tag="PK288_01565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPM17631.1"
                     /translation="MNQSRISVRYARALFQSALERNELDRIGRDMTLVKDTCKIPEVR
                     EILDNPVIMPSKKIRALRSLFGTELHELTLSLIDLVVRNGREQYLPAIARMFNQETKR
                     HRGITESVLTTAVPVDPGIRKQVEEMIEKMFNTRVELKTNIDESIIGGFILKVEDSYI
                     DASVRNKLRKIKHELISGSL"
     gene            21462..23045
                     /gene="atpA"
                     /locus_tag="PK288_01570"
     CDS             21462..23045
                     /gene="atpA"
                     /locus_tag="PK288_01570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPM17632.1"
                     /translation="MASIKPAEVSKILRQQLEGIQTGVELEEVGTVLEVGDGIARIYG
                     LSNAESNELIQFENGIEAIVLNLEEDNIGAVLLGPTEEINEGDIVKRTGRIASIGVGE
                     GLLGRVITPTGQPLDGKGPIEGELFEMPFERKAPGVIFRQPVKQPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKTAIAIDTIINQRSNFEKGKPVYCIYVAVGQKGSTVANIART
                     LEEHGAMEYTVIILATASDPAASQFYAPFAGAAIGEFFRDTGRDALIIYDDLSKQAVS
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIIESDEVARQMNDLPESIRHMVKG
                     GGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNSGVRPAINVGISVSRVG
                     GNAQVKAMKKIAGTLKVDQAQYRELEAFSKFGSDLDAATLATLDKGAKNVEILKQGQY
                     APVPVDKQIAIIYCGTSGLLRDVPVNRVREFETEFLEYLDLKHRDVLDSLGEGTINDE
                     ITGILDKTARELVLKFKQQ"
     gene            23075..23956
                     /gene="atpG"
                     /locus_tag="PK288_01575"
     CDS             23075..23956
                     /gene="atpG"
                     /locus_tag="PK288_01575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPM17633.1"
                     /translation="MANLKAIRLRITSVRSTRQITSAMKMVAAAKLRKAQDKIVRLRP
                     YAGKLHEILVHLTQSLADADIKNIYSRQESPEKILIVAITSNRGLCGAFNSNVVKEVK
                     RLITETYHEQYKNGNVEFLAIGKKGYDSLRKMKLNIISENNELLNDLTFEKVSALAEN
                     VMNAFVSGDYDRVELVYNHFKNAAIQQLTTEVFLPVETISEEGKAAAHVDYIYEPDKK
                     SIVRELIPKSLKIQFYKAVLDSFVAENGARMTAMHKATDNATEMIRDLTLQYNKARQA
                     AITGQILEVVSGAEALK"
     gene            23990..24964
                     /locus_tag="PK288_01580"
     CDS             23990..24964
                     /locus_tag="PK288_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPM17634.1"
                     /translation="MFDQSALKEIVNNAIINLSFDIGSDRLIDPVKYVLSMGGKRMRP
                     VLSLMACNLFSDKIDHAVIPVTGIEIFHNFTLVHDDIMDNARIRRGFPTVHTKWNINQ
                     AILSGDVMAFITNECFLQSPAHLLPRVLKVFNRAAIEVCAGQQLDMDFERATYVSQAD
                     YIRMIGLKTAALIAASLKIGAIFGGADDKDAGHLYEYGKNLGLAFQIQDDILDVFGDM
                     KVFGKTAGGDIIEGKKTFLYVRANETGSFDQKKKLQDLYASNAFKPEDKIRMVTELYE
                     LLDVKSSSEKIVADYFKDALEHLGKVNISRERKEGLIGLTSSLAGRDQ"
     gene            25032..26540
                     /gene="atpD"
                     /locus_tag="PK288_01585"
     CDS             25032..26540
                     /gene="atpD"
                     /locus_tag="PK288_01585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPM17635.1"
                     /translation="MAKNIGKISQIIGPVVDVTFERQGNEMPDIYDALEIKRNDGTIL
                     VVECQQHIGENTVRTIAMDSTDGLRRGMDVVATGQPITMPIGEQIKGRLMNVTGEAID
                     GIGVLDKEGGYPIHRKPPKFEDLSTEKEVLYTGIKVIDLIEPYSKGGKIGLFGGAGVG
                     KTVVILELINNIAKAYSGLSVFAGVGERTREGNDLLREMLEAGVITYGKEFLKNMEAG
                     GWDLSKVDREALKDSKLALVFGQMNEPPGARARVALSGLSVAEHFRDGDEKSGGRDIL
                     FFMDNVFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGIMQERITSTRHGSITSVQAV
                     YVPADDLTDPAPATTFAHLDATTVLSRKISELGIYPAVDPLDSTSRILTPEIVGEAHY
                     NCAQRVKELLQRYNELQDIIAILGMDELSEEDKLVVHRARRVQRFLSQPFHVAEQFTG
                     IKGALVSIEDTIKGFNMIMDGKVDQYPESAFLLVGTIEDAIEKGEKILAQSK"
     gene            26650..26895
                     /gene="atpC"
                     /locus_tag="PK288_01590"
     CDS             26650..26895
                     /gene="atpC"
                     /locus_tag="PK288_01590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPM17636.1"
                     /translation="MKIEIITPDHTIYEGEIKSVRVPGRKGSFQVLKDHAPIISTLDP
                     GPVYIVEQDNREVTYNIDGGVIEVRANRIILLTESVK"
     gene            26906..28318
                     /locus_tag="PK288_01595"
     CDS             26906..28318
                     /locus_tag="PK288_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM17637.1"
                     /translation="MTADEFRKHAHEVVEWMARYMENVESYPVKSQVRPGEILERIPP
                     VPPAQPEPFSAFMKDLDEIIMPGITHWQSPNFFAYFPANTSPPSILAEMIISTLGAQC
                     MIWETSPAAAELEEKIMIWLRDLIGLPAEFEGVIQDTASTATLAALITAREKASGFRI
                     NEEGAGSTARLRVYCSEQTHSSVEKAVKISGIGRNNLVKIPVKPDFSMDEEKLEKAVS
                     EDMEKGFIPCCAFATIGTTGTTAVDPLRAIGEICSRYDIWLHVDAAMGGTALILPEFQ
                     WMLDGREYIDSFVFNPHKWMFTNFDCSAFFIKHPSLLLKTFEILPEYLKTRTRGLVND
                     YRDWGIPMGRRFRALKLWSVIRMYGVEGLREKIRRHIEFAAELAEMIAREPDFEVAAP
                     VVMNVVCFRYRPAGKSAGEINGINETLNHRLNDTGKIYLTHTVINGHYTLRMVTGQTN
                     VTMNHVRKAWELIKTTAAQL"
     gene            complement(28315..31221)
                     /locus_tag="PK288_01600"
     CDS             complement(28315..31221)
                     /locus_tag="PK288_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17638.1"
                     /translation="MKSARSALLLLLSLALVPAAAQYYQTGQEPSSIKWMQIKTDKFR
                     VIYPESYGSQGIEFTRALEDAYSGLMLLYPEMKFRIPVIIHNHTTTSNGYVAWAPYRM
                     EIYPTPEQNSIPLDPKTQLAIHELTHVFQMKSLDKGFTRVMSYITGQQFNGAVAALLP
                     LWFLEGDAVFSETLLTGSGRGRTPSFQKPLKALMLEKGKIYKYDKIVNGSFRDFVPNH
                     YYSGYQMMAWSYAKYDSALWKKALNLTANLPFTLNPVNLSLRANASLTKKRLFRETFD
                     TLQVLWTAEDSALGSVEYDIVNPPKGKKYINYYSPVRAGKDSIIAVRTSLDHPPSFVL
                     IRPAEGRERRIHTPGYSYPWNISYARGKIVWVESDSDPRWRNRTWSVIKILDLKSGTT
                     RKLSGKTRYMAAAISPDASRIAAVRSSITQESGLVIIDAADGSILGEIPAPPGVWLQK
                     PQWDEKGNIITFVSLSEEGEGIMSFDLRKYAWKIHISPSSNDIQSAFTRNDSLFFVSS
                     ASGTDNVYLLAGDGKISRLTKSRFGVSDLNISGNNIMFADYSTSGNNICQASLRSEEP
                     DTAAFSENPSYLINRFRPYDGSSGESQPRVFLPEPYKKWKNLFRFHSWMPFYASIDEI
                     TSDPALIKPGITLMTQNNLSTLISTIGYEYADNRHMLHARINWLGWYPALESVIDYGY
                     RPVIEKLGENVPDPAEYNQGMAITNTLYLPLYFQSGKFSEHLRISASWKYINDHIYMK
                     NSGYYDPGQSRFTGRIYFSNYYRTAVRDIYPRWAQTMDLSYSAFPFDRELYGDLATLK
                     TAFYFPGLSGNHGLRVRLEKETQNPERFLQKNLISFSRGYDNIISKRADFVSADYYMP
                     LFYPDFNLSSFLYLTRIRADLFFDYTHVTDHYRLMRTDNGMIWFFTEGKMTFRSFGAQ
                     LLGDFFIFRIPYMVSAGVEAAWREPGTFPVIRLLFNMDIYGMSIGRNRTRAYNL"
     gene            31358..34567
                     /locus_tag="PK288_01605"
     CDS             31358..34567
                     /locus_tag="PK288_01605"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPM17639.1"
                     /translation="MKSYRKINNITGWVIFAVSAVTYLLTLEPTASLWDCGEFIASSY
                     KLEIGHPPGNPVFMVIARFFTLFAGGNVSTVAIMVNAMSALASAFTILFLFWTITHLA
                     RKLIIGNGDSTPGRIMAVMAAGATGALAFTFSDSFWFSAVEGEVYATSSFFTAVVFWA
                     ILKWEDVADEPHADKWIILIAYLMGLSIGVHLLNLLTLPGIVLVYYFRKFEFSWKGFL
                     ISLGISAVLLSLLMYGIMPGLVTISAKLDIFSRNTLGLPLNSGLIFHFILLIVTFILA
                     VRMTIISNDRTRISVFAAAAFFLSGIWVISGSAFLNIVLLGSVIFLAWYLAGRYKVVL
                     NTILTAVMVILIGYSAIAIIVIRSSAQPPLNENQPDNPFSLLYFLNREQYGQRPLFRG
                     AYYNAPVIDYEDGKPKYSIEDGKYKITAYNLETIHDTRFITLFPRMWSDQPDHARVYQ
                     EWGKIKGVPVTVTDRSGEKKLVRKPTFRENLRFMFTYQFGYMYFRYFLWNFSGKQNDT
                     QGTGGALNGNWITGIRFLDEPRVGTSDLPPGMKDDTSRNKYFLLPFILGVVGIFYQLN
                     RDIKNWSIILLLFLMTGVAIVFYLNQYPNQPRERDYAYTGSFYFYAVWIGLGVLAIFE
                     AARKIAGEKTAAIACGIVCLLAVPAVMASENWKDHDRSGRYLARDVAFNYLNSCAKDA
                     ILFTNGDNDTFPLWYAQEVEGVRTDIRVCNLMLLNTDWYINQMKWKTNESDPLPVSLP
                     ARKYYDGVNNQVFIVEKTKDPVDIKTVIDFVNSDNKSTKVQVSATELLDIIPARTIRI
                     PVDREKVLASGTVKPEDADKIVPWIDIKLTKSSILKSQLIVMDILANNNWERPIYYVT
                     GYHDDALGLEEYFQYEGLAYRLVPIKSEARSWIDYGRVDPDILYDNVMNKFVWGGANN
                     RKVNLDYNHIRTLMVVKARLVYARLAKELAARGDREMAASVIDRCMEVLPPDRFPYDP
                     YFPELIEAALKAGASDKAEEMTRAFTAYYYARLDYFLKQKPYIISSAEYEIQSAIQYV
                     SKVAGFCIENGQPALGAEIQERIGKYFSDYSSAEGIGIE"
     gene            complement(34572..35000)
                     /locus_tag="PK288_01610"
     CDS             complement(34572..35000)
                     /locus_tag="PK288_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM17640.1"
                     /translation="MSLNFSNLALEGKTILVVEDDVPSLRYYETLIQCSGANVVKLRT
                     GRDFVDYIQKDDAQIDLVIMDFLIPLINGIDCTKIFRKERKHVPVLMITAYSSEQSKA
                     DAFIAGCNEYILKPVYPEAIFTLLEKYLKQDVKEKVINLS"
     gene            complement(35157..35387)
                     /locus_tag="PK288_01615"
     CDS             complement(35157..35387)
                     /locus_tag="PK288_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17641.1"
                     /translation="MRKRIFYGVPMFIMMVFSLTSCEGLTGCKICRQVTYENGAVVQE
                     GREREYCGAELVAIEATEDIVSGNTRITWECR"
     gene            35540..35923
                     /locus_tag="PK288_01620"
     CDS             35540..35923
                     /locus_tag="PK288_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17642.1"
                     /translation="MEATKMKKHVTVVGAIHIGFGILGLIGALGLFFALSFAKGVVGD
                     EEIPSMLLGFLSLSLPILSGFMAIVGIAGGIGLLSFQVWARYLIIVIAALECLLIPVG
                     TLLGVYSIWVLLQDETIRLFTPAKT"
     gene            complement(36050..36916)
                     /locus_tag="PK288_01625"
     CDS             complement(36050..36916)
                     /locus_tag="PK288_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HPM17643.1"
                     /translation="MTGKLMKYAAFFLLPVLIAGCEKYIIFHSPDFEQKLVIVSFLSP
                     ADTDYFVSVTSNQPLRHFADHREDPGNITGTVDDGLYEYPLIKEGEGYHFRPGNMELI
                     PGKTYTLKVTSDKGLTAEASATIPRLRDLMIEIDTESVVHEGNGIYDDWREFIIKTEF
                     SDDPGEINFYSVTGRFTGYKTYEGFDTDVYKERLWFEESFLKDVRKDQNNRIKLETRI
                     SRSYQYYDSAFITVYVMNTEESYYLYHKSLLEYNGSDNPFSEAKPLYSNIRGGLGIFT
                     SYILDSLMFRLK"
     gene            complement(36919..39333)
                     /locus_tag="PK288_01630"
     CDS             complement(36919..39333)
                     /locus_tag="PK288_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM17644.1"
                     /translation="MKSVLYNTFIHPLQYLALAFMIFHGNIISGQQSLRHTVSGFVRE
                     AGSGELLTGVNVYLPDHRTGTVTNTYGFFSLTLPEADSVEITASYVGFSPQTKKISLR
                     EDTELNFDLRPGTLLDEVEISAERAERLSQSARMSTISVPVAQIKNIPSLLGEKDVLK
                     VFQLMPGVQKGSEGSSGLYVRGGGPDQNLIILDDAIVYNASHLFGFFSLFNGDALKSV
                     ELTKGGFPARYGGRLSSVLEMNMKEGNKEKWHGEGGIGLISSRLTLEGPLEKNKSSIL
                     LSGRRTYVDLIMKPIIRAMEETDAGYYFYDLNAKINYDLGRRNKLYLSGYFGKDRFHA
                     RYDENDLKETAGLGWGNATATFRWNHLFSSRLFSNTSFVFSDYKFGVTYKLKSRIENE
                     NFRAEYFSGIRDLTLKFDLDYLPSPKHSVKAGGAMIFHRFTPYVFIEIDEPINLKKSR
                     KKISHGLETGLYAEDTWQLLPDLKLNGGLRFSHFLTGSRQYMFLEPRLSATWKFNGDY
                     ALKASYASMNQYIHLISYTGISLPTDLWVPTTERVGPQQSRQVAFGTVRDLKTPDISL
                     SIEGYYKTMNHVLGYKEGASFFQFDEMGDIREVNWEDNVTAGKAWSYGLEFLVHRKAG
                     RFSGWIGYTLSWTQMQFDSLNYGKKFYARYDRRHDISLVGTYRIKKNITLSGTWVYGT
                     GNAVTMPTSVYYAPDHLDLFPELKPESGLGSPFHNIYDVNEYSRKNNYRMAPYHRLDL
                     GIQFHRQKKWGERIWEFSVYNVYNRKNPFFYRFRYLSSGTTSGALVEQVSLFPVIPSF
                     TYSFKF"
     gene            39499..41181
                     /gene="ettA"
                     /locus_tag="PK288_01635"
     CDS             39499..41181
                     /gene="ettA"
                     /locus_tag="PK288_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPM17645.1"
                     /translation="MTDDKKIIFSMYRVGKTFPPHRQVLKDISLSFFLGAKIGIIGLN
                     GSGKSTLLKIIAGIEKEYQGEVTFLPGYSVGHLAQDPLLDENRTVREIVEEGIKEIAD
                     LVREYEAINERFGDPEVYEDPARMQQLMDRQAELQEKIDYNDGWNIDHKLERAMDALR
                     CPEGSARVNILSGGERRRVALCRLLLRQPDILLLDEPTNHLDAESVQWLETTLKQYHG
                     TVICITHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKAQRLEIEEKGNIKRRKL
                     LERELEWVRMSPKARQAKSRARLGAYEKLLNQDVKAKEEKLEIFIPNGPRLGDKVIVA
                     KKVAKSFGEKLLFDDLGFNLPPNGIVGVIGPNGAGKTTLFRMIIGQEEPDSGVFEIGE
                     TVVTGYVDQAHSEIDPMKTVYEVISGGQNEVPVGNRLVNARAYAARFNFTGADQEKKC
                     GVLSGGERNRLHLALTLKSGANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVIISHD
                     RWFLDRVATHILAFEGESKVVWFEGSYSEYEENYRKRVADTTPVRIKYKKLV"
     gene            41292..42452
                     /locus_tag="PK288_01640"
     CDS             41292..42452
                     /locus_tag="PK288_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17646.1"
                     /translation="MKRLAFHKLLVSFAIMALPAPSLTAHIGSRFPSEKKTVEDKITG
                     AQLTFLTSQGPGDFGICNTGRQWTADGNWIIFRSNRAPGETLAINEKTGDMVQVTEGS
                     YMGVTAVSDKSMKLFFMRMTLRQPGQSGGGPVQIVEVDLERLLADSESGDVRDESEYQ
                     KVRGTIPAGMEAGGSMSIDAGGEWLYFRVGRAEASKHAAQVNPADRGYSQHPAGEILS
                     GISSFNIRTREIRFVLGLPFRVGRIRADQKVSGELYFCGEKDGTNPLQTWSVRSDGTG
                     LRTVCGGPQPKWPAGSGSVLMHHPASSDDRLASGSDAGGNIYLADLHSDKTILLSAGH
                     KTTSADPPCPSVSPDETKVLILSAMLSSDTRRLNLCIIKIPDGWQGSKQTGY"
BASE COUNT        11544 a         9676 c        10341 g        10987 t
ORIGIN      
        1 gctgtttcaa tagtggggca aaacctgttc ttcctttaca aaaaagcgcc ctatgacccc
       61 gaactgacaa tgagcaccgg cctgggaagc cagtcacttg acaacttcaa cgtcccggcc
      121 accaggactc ttggttttaa cattaaactt aatttctaat attgtgataa ttatgaaaaa
      181 aattatatcg ttagcagtgt tgctggtttt acttggggcc tgtacgaaga attttgaaga
      241 gatcaacagc aacccttacc agatcactga tgaatcactg acgcaggatt tcaatcacgt
      301 cggggcatat ttccagggat tgctgtccaa tctccagggc caccaggtcg aggaagacct
      361 tgcaaccgac agtttcgtac gccatttcgg cacaccgacc ccatttgttt cgaacaggaa
      421 caataccacg ttctatatta cctggaaccg tttctgggga cgaatctata acagtgtgat
      481 gtctccttcc aggcaggtga taaagattgc cgaagaaggc ggatatgacg ttttcgcagc
      541 atgggcaaaa ttcatccagg taatggggct ctcgaggctc acagcctacc acggcccgct
      601 tatttactcg gaatatggca agccgacaac tcccatcctg tatgactctg aaaagaccct
      661 gtacgattct tttttcaggg ttattgacga aacccaggct gtgtttgccg caaacaaaac
      721 ctacccgggt ctcaagaaat ttgatgcaag ctacggcggg gacatcacca aatggatcaa
      781 gcttatgaac tcaatgaggc tgaggctcgc catccgtctg agcaaagctg atcctaccct
      841 tgcaaaacag cagggtgaga aggcaattgc tgatgccgga ggcctgatcc ttacaaatgc
      901 cgacaacttc aacatttccc tctatggtgc aaaaatgccc ctggcagtta tctgcttcga
      961 atggcaggac accaggatgg gtgcgggtat ggaagaattc cttgtcgggc tgaaagaccc
     1021 caggatcgga aaatactttg cacccgcaaa ggatgcttca ctgtatactg accatcctgc
     1081 cttcccgtac aaaggaattg caagcggcgc gtatctcgga gcaaaagatg acaggcagct
     1141 gtactcggaa gtcagtgaag actggaagtc cgttacttcc agaagattct tcaccgcagc
     1201 cgaagtgcat ttcgcccttg ccgaagcagc attgagaggc tgggcaggtg caggcgacgc
     1261 caaaacccat tatgaagaag gaattaaaat gtcgttccag aactggggtg caggcggtgt
     1321 tgatacctat cttgcagatg ctaccagcac tcccattaac tacgttgatc ccaaggattc
     1381 aagaaacagc tacactaccc gctctacggt taccgttgcc tgggatgaaa ctgccacaaa
     1441 agaggagaaa ctcgagaaga tcataaccca gaaatggatc gacgcactta ccaatgcaaa
     1501 cgaagtatgg tgcgatcaca ggaggaccgg ttatcccaaa ctgcattatg ttcccaagaa
     1561 cgacagcaac caggactggg gtattatccc ggccgatggt ttccttcaga gaatgccgtt
     1621 tgtcgatgct gaaaggaaca gcaatcctga gggtgttgcc gatgcaacaa ccaagcttgg
     1681 aggccccgac cttatcagca cccggctcta ctttgcaccg gccggaccta acttcccgga
     1741 cgaaccctaa cttccaggat tttagttaat ccgctataat tcagggaggg tatcgcttaa
     1801 acgataccct ttcttttttg atcaaagccc atctttttcc cttcgcctcc gcatatcagg
     1861 atccggaatc cggatttgtt ctcctggcat ccaacatttc cggacataag tccctttgta
     1921 tcctttttcg tggcaagtaa aacacctccc tgatcatttt tttatgtcag aataaatgat
     1981 atttttgctg atgaagtgtg atttttaaaa tatcccggca aaaatatctg ttgccaaaaa
     2041 acggggaaac aatgttttta agccggattt aacaaaatcc gtttagtgca aggattgtat
     2101 aaatatttcc tgtcgtttgc aggccggctg acctatcctg gtaagatacc agtaaccata
     2161 aagatatcac cctagtatta acctgaataa agtagttatc atgaaaaaaa atctgatgtt
     2221 tctgttggtt cttgccattt ccactcttac tgtcatggcg cagcttgatt taaaaaagac
     2281 gggtacccag atcaaaaagc aggccgggca gctcagtaac gagaaaaagc ctgccgaagc
     2341 accggcatca ggcggcgcaa gccaggccgg tacttcaact gccacatccg gacccagcaa
     2401 gacattgtat gtttcttcaa caggaagcaa ccgcaacgat ggcagcaggg aaaaaccatt
     2461 taaagatatt cagaaggcca tcgatgaagc tcccgacggt gctgttattt gtgtaacaca
     2521 gggcaattat cttggcaagc tcgatgcagg ctacatctca attaaaaaat atatttcgct
     2581 ggtgggcggg tattctgaag atttttcgga gcgcgatccc gtcaggtatc gtacaaccat
     2641 acaaccaggg ccggaggcag gcggaacaaa cgcaaacttc ggactgatcg atattgatat
     2701 acgaaccaat cccaaaggca gggtgctaat cgacggattc atccttgaca agggacagat
     2761 gaaccgttat gtcgggctca atgcaaatga tgcaagattc aaggctcccg agggtgttga
     2821 aaccggtcat ctcaaccctc ccggaaaaca gatcggccaa ccgtcaatgc gcgggccaag
     2881 cacagtttcg aaccagctta tccatggcta tgtcaaaggc gatctcattg tgcgtaactg
     2941 tgtcttcctc aacggcagcc attttggtat acaggtagga aacattggcg ggcactttga
     3001 aatttacaac aatatattcc ttgcaagccg aatggcggca tgcgaaatat acagcatgaa
     3061 caatactccc ggggaggctg ttctcgattt ccaccacaac actgtccttt tcacatggcg
     3121 ccgcgactgg gtgccgggag acaaggatat gggctacgga ttccgttaca tgaccggtat
     3181 caatgcgaat gtcaacaaca acattatagg atgcagcgat ttttctgccc tcgaccggac
     3241 ccgtatcgat tctgacaaaa acagggaggc ccagcggaaa acctctgcaa ataacaacct
     3301 gttcttcggc aatattgaag ccgacctgac attgccttcc ggcggcggaa aattcatgcg
     3361 catttttgca aggcagtttg aggatgttga tcagctggcc gaggccgaag gtaacaggga
     3421 aatgagcgaa gctgaaataa aaatcctgag cagggcgatt gatgcagcat acctgaaagc
     3481 atttcttaat atagagggtt cctcatcgtc aacctacaat ccgaactctt ccgaaaacct
     3541 gttccgctca gcgatgggca tgaaccagcg cggcaccgaa acaaactatg tttccatgta
     3601 cgccaattca tatccgttcg ataaggctac cgatcttttt ggtgcccttg aaggctatgg
     3661 agcccaaaag atcagggatt aagaattgat taaggaataa aacgcaggca gggggcatga
     3721 tacttccttt tccggcattt tacccggttt aattatgaag gcttttccaa atgaactgga
     3781 ttcaggcttg tcctttaaag gcaatatttg catgatagga tgcggcagta tcgggcaagc
     3841 cttcctgcct gtcatcggca ggcatcttgg gggaatatac agccgtttga ccgttctctc
     3901 tgcctgcgaa tcagggcgaa gggtggcgca aggccgcggc agatacttcc ctgaaaagct
     3961 cgatgtgcaa agtccctggc agctgcagaa tatccgcagc agacagtatc atggggagtg
     4021 agaaaaacta attgcaggat gatagccttt tttatcacta tcccctttac aaatgaaatc
     4081 aggattccag gtaaccggtc tgcctatatg caccgaaaac tgcagagtcg tgccctgctg
     4141 aacataaagc caggaaataa ttttccttct ctgaaatact gacttaactt tgaccagacg
     4201 ttcgatcatg tgtgattcca tccggacatc tgtcagtatt atccagggca ggcccggatg
     4261 atgcagttaa caggataaca ggatttccca aatgaccagg gatgagattg atcttaaaaa
     4321 atatcatctg gctgactgtt cacggattta ttatgaatcc tgccttttcc cagggcatga
     4381 ctgcagctga tatcgtcatg aaagccgacg aaaaattcaa tggcgagaaa agcagttata
     4441 gtataatatc gatgacaatt gtacggccgt catggcagag gaccattgat atcaagacct
     4501 ggagcatggg aagggattac tcccttgccc tggtgaccgc tccgccgaag gaagcaggac
     4561 agtctttcct gaaacgaggt tcagaaatgt ggagctggat tccttctatc agcaggctcg
     4621 taaaattgcc tctttcaatg atgtcgcagg gctggatggg ttcggattat acaaatgatg
     4681 acattttacg ggaatcatca ctggttaatg attacttgca tgagattgcc ggggaggaaa
     4741 taatcgacgg ccgtttatgc tacaaaatat caatgaaggc caggcctgac gcttcaatag
     4801 tttggggcag gcagacaaga tggattgaca ggaaggattt tcttgttctg aaagctgaat
     4861 tatatgatga agacggctac ctggtaagaa ccgaaactgc atctgatatc cggatcatgg
     4921 atggaagaac cgtaacaacc cgaatagaac ttgtccctgc agaagaaccg ggaaacaaaa
     4981 ccattctggt gattcgtgat attaaattca atttaccact aggcgaaagt tttttttccc
     5041 agcagaatat gaaaagggtg cgctaacgga acggtcaggt atgaaagagg aattcaggct
     5101 ggcatggaga aatctctgga ggaacaggcg ccgcacggtt attactgcgg catctgtatt
     5161 ttttgcagtg ttctttactg ttataacccg atcgcttcag cttggttcct acgatcacat
     5221 gataaataat atcatacaat cgtttacggg gcatctccag gttcagcata tcaaatacca
     5281 cgatgatccg ttgatcgata attccttcgc ctacaatgat tcattgattt cagcgatttc
     5341 cgccattgaa aaggttgttt ccgtcactcc tcatattgag tcatttaccc ttgcctccaa
     5401 cggaccacag acaaaagggg tggcagtgat cggtatcgat cccgcaaggg aaaagcattt
     5461 ttccgatcct gaagcaaagc ttgtcagata caggataacc ggagaagctg tggaaggcat
     5521 gagaaccgga gagttacctg aaaaggtcct ggagaaagtg gaaaagaaca ttggaagatc
     5581 ctattcatcg gctgccagga tcgagcttga acttgggctg accgacgagg aaaaaaatca
     5641 ttgtctttcg cagatcctta aatattctga atttaaaaac ggctatttgt ccccggatga
     5701 caagggaata ctgcttgccg acaagcttgc aggatttctc agggcgggga tcggggattc
     5761 aataatcctg atgggacagg gatatcatgg tgtttctgca accggtatct tcccggtcag
     5821 gggaataatt aaattacctt cccccgggct tgacagccgg cttgtcataa tgacaatacc
     5881 ggctgcccag cagctctttg atgcagatgg aaaaataacc tccctggtga ttaatcttaa
     5941 agaccggtcg ggcaggacca taacaaaagc aaggaataag atcaacgcgc ttctgcctga
     6001 cacgaacaca tcagccagaa catggtacga actcaacgag gtgttatatc agcagatgag
     6061 gggcgacagc cagagcggaa agctgatgct gcttattctt tactttatca tttttttcgg
     6121 catattcggg accgtcctga tgatgatttc cgaaaggagg agggaatttg gtgtactggt
     6181 ggcaataggc atgcggaagg ggaagctgaa aaggattgtt ataattgaaa tggtcatgct
     6241 tggcatcatc ggtctgataa ccggcctgct tgccggcacc ccgttgataa cctacttcaa
     6301 ccacaatccg gtgataatta aaggggatct cgggaagatg atggaagact ggggatatga
     6361 tgcaatgatg cccactgcgc cagtcggatc atacttttac tggcaggttg tgatcgttat
     6421 cctgatgata ttgcttgcag ccatttaccc gttgcggaaa atcggaaaac tgaaggaaac
     6481 agaagcattg aaaagttaat ctgccgtcat ctatgatatg gacactcgca tggaagaata
     6541 tctggcgtaa caagaaaaga agcctggtgg taattttcgc ggttaccctg ggaatcattt
     6601 ccggaatgtt ccttatagga tttgtggaag gctggagcag gcagcgcctg cacgatgcca
     6661 tatacaacga ggtgtcgcat ttacagattc ataacagtga gtaccccgga aatgaggaaa
     6721 ctgaccttac ggttataaat cctgtccggc ttgttctgca tattgattcc ctgaaagagc
     6781 tccgaagctg ggcaaaaagg accaggatcg ttgccatggc taatacaccc tggggaaaca
     6841 cgggtctgac cttattcggt attgatcccg aaagggaaaa actgctgacc gaaatccacg
     6901 acaggattgt tccgggcgga ggcagataca ttgattccgg aaatccttcg gggatcctga
     6961 tcagtgataa aacggctgat ttgctcaggt taaagcatta tatcctcact gacagcgtaa
     7021 tggaagaatt aaggcatgaa aagataccgg tttcagttct tgaaagaatc aatcagctta
     7081 ggaatgtccg cttccggtca ttaaaggatt tcagggaggg tctgatgccg ctactgaccg
     7141 ggaaagagac ggccaggtat ggctttctga tatcaggaaa ggcgctgaat taccggctca
     7201 ggaacagggt ccaggtcaca atttccgata ttgacgggaa cccggttcag ggaattttca
     7261 gggtgtgcgg ggtattcaag actgccaata cgggctttga tcagtcagca gcctttgtaa
     7321 atgccgcgga gcttgcccgg caatacggga gcggggagat tcctgttcat gaaattgcca
     7381 ttttgcttaa tgacattgat gatgctgaca tggtaaaaaa cacgctgaac gccgttgcag
     7441 gaggaaattc agtcatgacc tggaaagagc tggctccgga agcggctctg atgaacgagt
     7501 tcatgtttgc ttattacctg gttttcatcg ggataataat gcttgccctt gcattcggga
     7561 taatcaatac catgctgatg gcagtgcttg aaaggacaaa agagctgggc atgcttatgg
     7621 cgattggcat gaatcgcaga aggatcttta caatgatcat gctggagacc attttcctga
     7681 ccttcgtggg ggcagcggca gggaccgttg cgggcctggc acttatcgct gtccttggaa
     7741 aaacgggaat ccatcttggc agctggcgtg agggatttga agcaataggg tttgctgcaa
     7801 cggtttaccc gtttgttact cccgcgttta ttgtctttac cgtgatcatg gtcacggtca
     7861 cggcagtaat ctcctcaata tggccggcga gaaaggctct gaaactgaac ccggttgagg
     7921 ccctcagaac agaatgaaat atttccggca gacggattca ttgattcccg gatcaatatt
     7981 gcgcaacatt tttcgatttg tttaagctca aaatagtgaa acatgaaagt acttgaactt
     8041 aaaaacgtga ataaaatata caattcatcg gaaattaaag ttcatgcggt gaataatgtg
     8101 actcttgact ttgaagaggc cgaatttgct gccattgtcg ggccttcagg atccggcaag
     8161 accaccctgc tgaatcttat cggagggctc gatatgccca gctcgggtga aataatagta
     8221 ggcggcacca ttctgtcgga gcttaagcca tctcaactca ttgatttcag gctccacaat
     8281 attggttttg tatttcagtc gtttaatctc attcccgtac ttactgccag ggaaaatgtt
     8341 gaacttataa tggtgctcca gaaatggccg gagaataaaa ggaatcttcg tacgattgag
     8401 ctgctgaacg cggtgggtct gggcgacagg ctgaacagcc ggcctgccag gctgtcgggc
     8461 ggagagcagc aaagggtggc agtagccagg gcactggcat caaaaccaaa attcgttctt
     8521 gctgatgaac ctacggcaaa cctcgattca aaatcagcag ctacactcct tgaaataatg
     8581 gtaaacctca accgtgaaga gaatgttaca tttatttttt caactcatga cccgagagtg
     8641 gtgaaaatgg cacggagaat cattattctt gaagacggaa aaatcgtctc ggatgaaaaa
     8701 caatgtcaat gactttatgt cgataaatca gagaatagta ctgtgcctcc tggcagtgtt
     8761 tatattcttc ccgtctgttt cccaggaaaa ggaaaggaag attgaactga aaggatactt
     8821 ttcaaccctt cagactgtga ttttcgattc tctttcaggg ccctttgcca atgagaatct
     8881 gcttcataac aggctcaatt tcaaagcata cctgaatgat catcttacaa ttggcgcaga
     8941 attccggaac agactgttta ccggcgacct gacgatgctc ggaacagctt ataccgatct
     9001 gatcggaatg gaccggggtg ttgtggatat gtcatgggac ttactcaggg aacagtcaat
     9061 ctaccttaac agcaggatag acaggctgtg gatagactat aactacagga aaatccaggt
     9121 taccctcggg cgccagcgga taaactgggg gcaaaccctg gtttggaatc caaacgatat
     9181 tttcaacgca tattcctttt ttgattttga ttacgtggaa aaacccggga gcgacgcact
     9241 acggctgcaa tattacccgt catcctcttc cgtggcagaa ctggcgttta aactcgacaa
     9301 tgaatatgat ctgactgctg caggattgtt caggttcaat aaattcggat atgattttca
     9361 gtttctggcg ggactggtaa acagcgagga tgtggttatg ggcgccggct ggtctggctc
     9421 tgtcgggagt ctgtctttca ggggagaggg ttcatggttc agtccgtatg agcaatttcc
     9481 caaaagcagg ggaaccatac ttgtcacctc gggcctggaa aaggtcttta agaataactc
     9541 atcagctgct attcagttga tgtacagcaa caatcctctt ggtttcattg atttctattc
     9601 catcctggga ggtgagcttt cgtcaaagaa tcttgcattt tccgagttta ctgcttttgg
     9661 tcagttcaca tgggcaatca ctccgatgct caacctgact gtttcctcaa tgtggttccc
     9721 tgatctcgac ggatattatg caggtccgtc gctcgaatat tcactggctg agaatgtaga
     9781 tttttcggtg atctggcagc acttcgacgc cgtccttgcc ggggtaagga acatcataaa
     9841 cctcggattt ctgaggttca aatacagttt ctgaaatttt tatacttttg agccataaaa
     9901 ttttctgaac gaatccgatg gctcttgtcc acgaacttga aaaaagcggc aactggctct
     9961 ttaaaagaag aagctggctt cccctgattc tgatagccgc cggtatcgtg gttttatatc
    10021 atacgaatcg tcaggcaatt ctcttcaatc cggttaagga actgatcttt ctgggtgtaa
    10081 gtctgttcgg tgaggcacta cgggtttata ccgtcggttt tgccccgaga aacacttcag
    10141 gacgaaatac ttctgcgggt cagatagccg acgaacttaa caccacggga atttattcgg
    10201 tgataaggca tcccctttac accggcaatt actttatgtg gctggggccg gttctttttg
    10261 tgagaaatcc tgttttcatt cttttcttcc tgcttgttta ctggctttat tatgaaagaa
    10321 taatgtttgc cgaagaacag ttcctcaggc gtaaattcgg ggaagaatat gacaaatggt
    10381 ccgaaaatgt gaaagctgtt atcccgtgtt tcaggaaatt caggaaaccc ttgatggcat
    10441 tttcattcag atctgttctc aggcgggagt atcacagttt cgtcaatata tttattattt
    10501 tcctgatcct tgacattttc aggaattatt tcatttcgtc aagggtacag gttacgccaa
    10561 tgtggttatt tatttccatc gctgccggcc taatatggct cgttctcagg attatcgcca
    10621 aaaaaacttc ctggcttgag tgcgatgaca ggtaagctcc ggggtcagtc cctgctgctt
    10681 cccatgaaaa tcatgatata gtacagcaat gttgcgagcg accctaatgc tgccacaacg
    10741 taggtatatg cggcccattt cagtgcatcc cgcgctttgt cgtgattcat cgtcgatgtg
    10801 atacccgtat tggagagcca tgccagtgcc ctctggctgg cattgatctc aaccggcagc
    10861 gttacaaagc tgaacagggt tgtaagagca aacagaatga ttcccgtaag cagaagtgcc
    10921 ggaaatgtat taaccaggat tattcctcca agcaggatcc actgaaccca ttttgaagca
    10981 aaggttacgg ggggaacgag cttcgacctt aagcccagcc aggcataagc cgtcgcatgc
    11041 tgcacagcat gcccggtctc atgagccgca acggctgcag cagcgatact cctgccctca
    11101 tacacttcgc ggctcagatt aatggttttt ttgtcgggat tgtaatgatc agtcagctcg
    11161 ccctgaacac atcctaccgt tacatcataa attccattgt cgcgcagcat cctttctgca
    11221 acttcacgac ccgacatccc gttgtcgacc ggaatcctgg aatactttct gaactttgat
    11281 tttaataccg agcttattat ccagctgaga atcatgaaga cgccaaaaat cagccagata
    11341 ttcatatttg catgctgcat ttccataatt gtatagtttg attgttatta tcctctaaaa
    11401 ctcattcaag gttaaaacta ctccaaaagt cctgccagaa catggccgga tagctgaaac
    11461 tggtcatttt gtcagtttta aaacattgac tgttacatct ctgatcataa gcggcaaatt
    11521 tagtatgtca tttgttatct ttgttcaatg ggaaacatgg ctgaaaatat cattgtaagg
    11581 atactttccc ttcctgtgct gttctacaga cattcaatct cccctctgct gcgtccttcc
    11641 tgccgtcatg tgcctacatg cagccagtat tcgctcgatg ccctcagact gcatggtccg
    11701 gttcacggat tgctgctggc cgcaaacaga atattgcgtt gcaggccggg cggaactcat
    11761 ggctatgatc cggttccctt attcagattt aaaaggtacc ggagcttccg cctgtataaa
    11821 aagtgcagca ggctcaggta agccggatat tgctgcctgg cgggctggtg ataatggtaa
    11881 taaggtccgt atcagtaatc gagaacttcc ttcaggttcc tgagtcccga acggcttaca
    11941 ggcagcctgg cacctgaaag gagaatggca atatgattgt cttttccgta aggttcaatg
    12001 ccctttatct cttcaacctt cacaatgtat gacctgtgga tccttacaaa atcctttttt
    12061 gaaaggttat cctcatagaa tttcattgta tgctgcttca gggccttccc cgatgaatga
    12121 tggatcataa cataatcatc ctgggcttca acatatctta tctgatccac cgggatgatg
    12181 ctgattgcgt ttcccttccg caccacgata cgattgaccg gggaatcggg atcgggccgt
    12241 tttgacagaa gcaggctggc cggtcctgtg tttcctgctc ccgctctgat tttcagggtc
    12301 gcttttttca ctgcttcaat aaagcgtctt ttggaaaagg gcttgagaag gtagtcaatc
    12361 gcgttgagtt caaaggccct gatcgcaaaa tgatcgtatg ccgtggtgaa gattatttcg
    12421 ggcttttcgg tcatcacctc aaccagttct attcccgtaa gcctgggcat ttgaatatcg
    12481 aggaacacca ggtcgggctt ctccgaaaca attgttttca ggccggcgaa accatcactg
    12541 cattcagcca ctacttcaat ttcctccagg tccgtaagat aatgcctgat caagtccctt
    12601 gcaggcgctt catcatcaat tattactgct ctgatctttt ccatggcaaa ccaagggtcc
    12661 ggccggatgg cgcagggcag accggatttt atttcaacta tacaaattta gttatttctg
    12721 attatcctca acattggctg gcagaacaag tgttaccgaa aagatgccat tttcccttgc
    12781 tatcctgact gatgctttgc tgccatagaa aagatcgagc cttctggtca cattcagcaa
    12841 tcccgttccg gttcccttaa cgggcgaggg aacagtatca taattgttgc ttataactat
    12901 ttcaagaaat ccttctgagc atctaaccct ggtattgatc ctgacaattt cggtgctttc
    12961 atacacaccg tgcttgactg cattctcgta gaggggctgg agcagcatca ccggcagttt
    13021 tatgtcaagg cagttttcat caatctcctc tccggttgat agtttgtcgc caaagcggat
    13081 tttttcgatc tcaaggtaca atttaaggtt ttcgagttca ctccggagtg tcacgggttg
    13141 ttcatccttt ttggaaagtg tatatctcat gaactcggat aattttatta tcatttccct
    13201 ggctttgtca ggatttgtaa ctgtaaggga gctgattgaa ttaaggctgt tgaagaggaa
    13261 atgtggattg atctgtgatc taagcgcttt cagctcggta tccctgacaa gggtttccag
    13321 gcgggcttcc cgtgcattct tttccgaaag gttataaaga gtttcaaaaa gataataagc
    13381 caggataatc aatgcgtaaa ttaatccccc ggtaccggcc ctgtagggga atgttgcctg
    13441 ccagtaagct tcaaaaccgg aattcacggg aaggaacagg ggtactgcaa accgggtgac
    13501 aatgatccac agggctactg ataatgtgcc gccgatcaga atgttcccgg caactgaaat
    13561 caatgctgat ttacccgcgt ttatgaacct gaaagggtac cagagtgaaa tccctatacc
    13621 cgaaaaaact atcattgaaa taattccgtc gggaataaaa atctcggtta tttctccgta
    13681 tgcaaaatag tagagaaaaa gctggccggc agtaagtaac agccaggcca gccaccatat
    13741 aatcaatcta accctgtcgg aaagtacggg atgtctcatt tgttcaggac tagtagctct
    13801 ttatttcccc tccgccgaaa accacggctc ccttaatcag cagttgtttg ttcttgtcaa
    13861 tggtcattcc gggtgtcagt ttccgggaat cgctgaaccc gccaagaaca ggtgtcacgt
    13921 cgatggttac aaaccagtcg ttgggcacaa ttatcgttgc tccgccaaac acgcatgcga
    13981 cctccagtac tgacactccc tgcaccagat cggctttggt gagatcaagt tctattcctc
    14041 cgaaaatggc ggtgatctta cctccctgga agttccggga agttacctgt ctgtcgcctc
    14101 cgctgaaaac attaacataa tcgacataat catcacctgt aactcccttt gaagttgatg
    14161 taccccatcc ccttcttttg gttgtaacga atattatacc cactataata aaaactgcag
    14221 gccagaacat gttgtacatg tgcagggttt ccctgaatgc cagtggtatc atgaaaaagc
    14281 cgccgactgc cattacaatt attcccgcgg ttttttcagt tgcattcaac gtcatcacaa
    14341 ggccaataac aataaggagc atctgccagc tgaatattat gttatctatg aaatcaggaa
    14401 aaaatcctgt attccggatt acgagaaaca gtccggccaa tataagaacg acaccaatga
    14461 cagccctgtt gctggaacgg tgcggtccaa gctggcggtg ttcctgatcc ttaaaatcat
    14521 tatgtgtttc catggttcta aatatgttgg gattaaatta aatacttttc gtctttcttt
    14581 tcaccaatga cgagataata aagcccagtc cgataaggat aattactgcg ggccagtaga
    14641 ttgctttaat gatttccggc atttcataat taattttatc catcagaaac caggttcccg
    14701 ctgctatcag aagcagcccc ccggtaaact ggaggttcag tatgagcaga acacctataa
    14761 atatcatgcc tgtctgccag gtaaaataat cactgatctc acccaggttc agcagatcat
    14821 tcgtggcaac cagcagggcg aggcccaggg taatcaggaa cagtccgaaa ccaacctgcg
    14881 gatggtgctc cctgtgccgg agaataaaat ggtccttgtt tttcatggca tcctctttta
    14941 ataaatcgtt cctgcgtcaa aaataacatt tgtccggggg agcctgttag cataaatcgg
    15001 tgagttgcgc taaaaaaccg gtaagcagcc cattaaggca ttttttatga gatttttatt
    15061 gataattttt aattttgatg aaggatttat atttttgcaa tccaaattaa aaaggatgtc
    15121 cggattgtat ctgataccga ttggagggtt aaaggaagga tatcgctcat ttgaatttga
    15181 gatagacaac agttttttcg gtctttttga ggaatcggaa attaaagagg gaaaactcac
    15241 tgcagttgtt gaaactgtta aaagtccttc ccttattgag atgctcatca ggatctcggg
    15301 gaccgtaagg ataggctgcg accgttgtct tgaaatgttt gatgaggttc tgaactgtga
    15361 gaacaggctg gtggtaaaat tcgggaacac tgccgatgta gcggatcctg aaattattat
    15421 cttaccctat ggtgaaaatg agctggatat gaagcaattc ttttatgagt tcatccatct
    15481 tgcccttcca atccagagaa tacatcctga tgatgaaaat ggcaacagcg ggtgcaaccc
    15541 ggaaatgctc agtaagttaa gggaacacct tgtcaatgag gaaagcctgg ttgatccggg
    15601 gtgggacgaa ttgaaaaagt taatgaacaa caattaataa agaaataaaa gatggcacat
    15661 cctaaacgaa aacactccaa ggccaggcgg gataaacgca gaacgcattt taaagcaacc
    15721 cctcccacaa ccgggacatg ttcaaactgc gggtcttccg tccagtatca cagggtctgt
    15781 cctgagtgtg gttattacag gggtaaacct gccgtggaga agaaagctac agcctgatct
    15841 tgtcaacaaa aagctgaaga atgaggattg gatttgatgt aatgggtgga gatttcgcac
    15901 cagaggctat gattgaaggt gcagttgact ccattagtta ttttggccct gacgaaaaac
    15961 tcgttcttat cggagataag cgggagatat tgaagaagct tgaagaaatg aacactgctc
    16021 cggatttgtt cgagatcgtc cattcttcac aggtaattga gatgggcgac catcctgcaa
    16081 aagctttttc ccagaaaaga gattcaagta tagcaattgg ctatgaaatg ctgaaacagg
    16141 gattgcttga cgggttttgc agtgcaggta ataccggggc catgcttgtc ggagcaagct
    16201 acacggttaa tgtgattccg ggtgttttca ggcccgctct ggccacaata ctgccctgta
    16261 tcgacggacg cgattccgtc atacttgatg tggggcttaa tcctgattgc aagcccgatg
    16321 tgctgctgca gtatgggatc cttggcagca tttatgccag gttcgttctg ggcaaaaaca
    16381 atcccacggt tggattgcta aacataggta ttgaagaaac aaagggcacc cctgctataa
    16441 aagctgccta tgaactgatg aaggattacc cggggctgaa cttcagcggc aatgtggaag
    16501 gcaatacact atttcatgag agtgtaaccg atgttctggt ctgtgacggc tttgtcggca
    16561 atattatcct caaacaggct gaagcttttt tttcaatatc ggaaaagtta aaaatctccc
    16621 atccctttat tgacaggatg aactttgaaa atataggggg cacaccggtt gtcggcatta
    16681 atgccaatgt tgttatcggc catggtatat ccaggagaaa agcaataaag aacatgattt
    16741 tgcaaacgaa agcggttgtc ggggcaaatc ttgcccggaa aataattgaa gctatctgaa
    16801 tatggaagaa gtacgggctg ttataacagg aattcatgcc tgggttcctg aatacaggct
    16861 gaccaatcac gaactttcaa aaatggttga tacttccgac gaatggataa tgcaacgcgt
    16921 cggaataaag gaaagaagaa ttctgaaggg tgaggggctg ggctcatccg atctcggtga
    16981 gaaggctgtc aagggtcttc tggagaaaac gaacacttca cccgatgaaa ttgatctcgt
    17041 catctgtgca accattactc ccgacatggt attcccggcc acggcaaaca taatttccga
    17101 caaggccggc cttaaaaatg cattcagctt tgatctgggc gctgcatgct caggtttttt
    17161 gtttgcactg cagacaggcg ccgcttacat tcagaccgga aggttcagaa aagtcattgt
    17221 tattggagct gataaaatgt catcgatcac cgattacact gaccggacaa cctgcccctt
    17281 gttcggcgat gctgcagggg ccgttcttct tgagccggga acaggggatt acggtattat
    17341 ggactatatt ttccgtgttg acgggtcggg caggaagttc ctgcacatga aggcaggggg
    17401 ctcggtgaag ccagcctcgc atgagaccgt taatgcccgt gaacactttg tttaccagga
    17461 aggccagagc gtcttcaaat ttgcagtggt caatatggca gacattgctg tcgaaatcat
    17521 ggaacgcaat aacctgaaac cggaagatat cgcgtggctg gtaccccacc aggcaaacct
    17581 caggataatc gacgcaacgg gaaaaaggat ggggctgcca cccgaaaaag ttatggtgaa
    17641 catagaaaat tacggaaaca caacggcagc aactattcct ctgtgtcttt gggaatatga
    17701 aaaaaaactc aggaaaggcg acaagctgat acttgccgct ttcggcgggg ggttcacctg
    17761 gggatccctc tatctcaaat gggcttacga ccctgattaa tgcagtccgg atttctcccg
    17821 gcacatttat actgatccgc ctcccttatc cgcttcgatt ctgccccggg atacctatca
    17881 acagttccgc gaacatcatt ctattttttt acaatagttt ttatatttgt gagttattat
    17941 gtttgtaagt ataattctgg caaatcatgg gaaaattcaa cgaaaccgac aatactgcaa
    18001 tcaatctgat cagcaatggc actgaaatca ccggggatat caaatcaaat ggcgatatca
    18061 gggtcgacgg gcttcttacg ggcaacctta ctacaaaagg gaaagttgta ataggaccaa
    18121 caggcaaggt aaagggagaa gtgtattgca aaaactcgga agtgtcaggg actgttgaag
    18181 gcaggatttg tgtgagtcag ttattgaccc tgaaagtcga ttcaaggatt ttaggggata
    18241 tagttacttt caagctttca attgaacccg gcgccaaatt cacaggcaac tgtaaaatga
    18301 gtgaaagcga aaataatgaa ggagccagct tcacaaaaac aaaagaaccc gaaaagtccg
    18361 tcaaataaag gtttacacga ttttgcaaaa tattccggcc tggccttcca gatgcttgta
    18421 ataattgcag caatgacctg gggcggagtg aaactcgatg aagttctggg attatcgaca
    18481 ccggtgttca ccatagtcct gtctcttctc ggagtatttg caggaattta tactgcaata
    18541 aaggacttta ttaaacgata atctcacaaa tgaatttcag aaaatacctg accggacttt
    18601 tatgggtttg cctgcccctg ctggcagcag ggtatctgtt atcccggttc atcccggtcc
    18661 catttctttt caccgatctg ttgttactca ctgtttcgtt ttcagcaatt ggcataactg
    18721 cggcactgat ctccaggtcg gggctgaaga agggagccga gggaggaaca atgtacctta
    18781 tggttgcact gtcggtcaaa ttgctactgg agatggtcct tgcacttctg tggtttgtag
    18841 ttgttaaaaa aacttatctg tcctcagtta tattgttttt tgtactatat ttggccgttt
    18901 ccctgttttc aattattttt atattgaata cactgaaaac taaaccttta taaaactatt
    18961 cttaatataa tgcaatttgt caaagcaagt ctgttattcc tgctttttac aataattcct
    19021 gctgccctca ccgcacagga ggaacacggc agtcatgcag gaagggcaga agaggagttt
    19081 gatgcaagca cctttatcat ggatcatata gccgattcgc atgaatggca cattctgacc
    19141 aggaagaacg gcgagagcgt tgcaatttat cttcctgtga tcctatactc tgctgaaaag
    19201 ggctgggatg tgttctcttc ccggcagctt gcacacggac atgaatataa aggatacagg
    19261 ctggaagagg aaggtgacaa caaaggaagg atagtgagct taaatgagga tggtacaatt
    19321 aatgaggaag ctttcctgct cgatctgtca ataacaaaaa cagttgtcgg actattgttt
    19381 tccgttataa tcggactctg gctctttctt tcattgtcgc gttcctacaa gaaaaccggg
    19441 atcagccatc ccagggggat tcaaagcttt ctcgaaccaa taatcctttt tgtgcgcgac
    19501 gatatagcga tccccaacct tggggaggat aaatatgaaa aatacatgtc ctacctgctt
    19561 accgttttct tttttatact gttgaacaac ctgatggggc ttgttccctt tcctccgcca
    19621 ttcggtgcca atgtaaccgg caatattgcg gtaaccatgg ttcttgcact gttcactttc
    19681 ttcataaccc agatcaacgg aagcaaatcc cactggaggc atgtttttgc aacaccaggg
    19741 gtgccggtct ggctccttcc cgtaatgatc ccggtggaga tcataggcct gttctcaaag
    19801 ccatttgcac tgatggtccg actcttcgcc aacattactg caggccacat tatcgtcctc
    19861 agcctggtct gcctgatctt tattttcaag tcgctgtttg tggcgccggt atcaatcgca
    19921 ttcgttatat tcatggattg cctcgaactt ctggtggcat tccttcagac atatgttttc
    19981 accctgttgt cggccctttt tataagtttg gccgtcaaag gagaggagga acatcattag
    20041 tttttattgt ttaatttaaa ccagttacta tcatgacagg tacattggca gcagtgggag
    20101 caagcctggc agttatcggc gcaggtcttg gaataggaag gatcggtggt tcagccatgg
    20161 acgctattgc acgtcagccg gaagccagct ccaagatcca gacaaacatg atcgtctctg
    20221 cggctcttat tgaaggagct gcattgttcg ccatcgtggt tgccctcatt gcaaagtaaa
    20281 ggaaacagga gggctgcaac ggttggttgc agcgcctccc tattaaatca aaagacaaaa
    20341 acataaagat atggtcttac tagcaaacag tctcacatcg ccggcaatag gcctggtatt
    20401 ctggacaaca ataattttcc tgatgttttt cctcattctg aaaaagtttg cctggaaacc
    20461 tattttaaaa gcggttaagg ccagggatga gatgataaag ggatctctgg catcggccga
    20521 aaaagcccgg aaagagatgg ccaggctcca gagcgacaat gaagccatcc tcagaaaggc
    20581 cagggaagaa cgcgaaataa tcctgaggga agccagggat atccgcgaca agcttatttc
    20641 cgaagccaag ggaaaagctt ccgaagaagc ggagaaggtt gttgcaaagg cacgggccgg
    20701 cattgaaagg gaaaaaatag ccgcactggg tgagatccgc aaacaggttg ccaccctgtc
    20761 aatagaaatt gcctccaaac tgataggtga aaagcttgaa aaatcagggg aacaggaaaa
    20821 gcttattgac aactacctga aagaaatcga cttcacgaag aactaatttt tttttaatga
    20881 atcaaagcag aatatcggtc aggtacgcaa gagctttgtt ccagtcggcc cttgaaagaa
    20941 atgaactcga caggatcggc agggacatga cgttggtaaa ggatacctgc aaaattccgg
    21001 aggttaggga aatcctcgat aatcccgtaa ttatgccctc gaagaagatc agggcattgc
    21061 gcagcctttt tggtacggag cttcatgaac ttaccctgtc gcttatcgac ctggtggtaa
    21121 ggaacggaag ggaacaatac ctcccggcaa ttgccaggat gttcaaccag gaaacaaaaa
    21181 ggcacagggg tattaccgaa tcggttctga ccacagccgt accagtcgat ccggggatca
    21241 gaaaacaggt ggaggaaatg atagagaaaa tgttcaacac cagggtcgag cttaagacga
    21301 acattgatga gtcaataata ggtggtttta tccttaaggt tgaggacagc tatattgatg
    21361 caagcgtaag aaacaagtta aggaaaatca aacacgaact gataagcgga tcattatgat
    21421 cctgaaaaca ataagacaag tatgatttta taggaacaaa catggcaagt attaaaccag
    21481 cagaagtttc aaaaatcctc aggcagcagc ttgaaggtat ccagaccggg gtcgagcttg
    21541 aagaggttgg caccgtcctt gaagttggag acgggattgc acggatatac ggtttgtcga
    21601 atgccgaatc gaacgagctg atccagtttg agaatggcat tgaagccatt gtgctcaacc
    21661 ttgaggagga caatatcggg gccgtattgc tgggccccac tgaggagatc aatgaaggcg
    21721 acattgtaaa gcggaccggg agaattgctt caatcggtgt aggagaaggg ttgctaggaa
    21781 gggtcattac tccgaccggt cagcctctag acggcaaggg cccgatagaa ggtgaactgt
    21841 ttgaaatgcc gtttgaacgg aaagctccgg gtgttatatt caggcaaccg gtaaaacagc
    21901 cgctccagac gggcataaag gcgatcgatg ccatgatccc gatcggcagg ggtcagcgtg
    21961 agctgataat cggcgatcgc cagaccggaa agacggctat agcaattgat acaatcataa
    22021 accagagaag caattttgaa aagggaaaac cggtatactg catttatgtt gcggtagggc
    22081 agaagggttc aaccgttgca aatatcgcca gaacgctcga ggagcacggc gcaatggaat
    22141 atacggtgat cattcttgca acagcttccg accctgctgc ttcccagttt tatgctccct
    22201 ttgcaggcgc tgcaataggt gaattcttcc gtgataccgg ccgtgacgcc ctgatcattt
    22261 acgatgacct gtcgaaacag gcagtatcgt acagggaggt ttccctcctg ctccgcaggc
    22321 ctccgggccg tgaagcctat ccgggcgacg tcttctatct tcattcacga ctcctggaaa
    22381 gggctgccaa gatcatcgag tcggatgaag ttgcaaggca aatgaacgac ctgccggaat
    22441 cgatccgcca catggtaaag ggaggcggtt cactaaccgc cctgccgata attgaaactc
    22501 aggccggcga cgtttcggcc tatattccga ctaatgttat ttcaataaca gacggacaga
    22561 ttttccttga atccaacctg ttcaactcag gtgtgcgtcc ggcaatcaac gttggtattt
    22621 cggtttccag ggtagggggc aatgcccagg tcaaggccat gaaaaaaatt gccggtaccc
    22681 tgaaagtcga tcaggcccag taccgcgaac tggaagcttt ctccaaattc ggatctgacc
    22741 tggatgcggc aacacttgcc accctggaca aaggtgcaaa gaacgtcgaa atacttaaac
    22801 agggccaata tgctccggtt cccgtcgaca aacagattgc aataatctat tgcggaacca
    22861 gcgggcttct cagggacgtg cctgtaaaca gggtcaggga attcgaaact gaattcctcg
    22921 agtatctcga tctgaaacac agggatgtcc ttgattcact tggtgaggga acaataaatg
    22981 atgaaataac cggtatcctc gataaaacgg cccgtgaact tgtcttgaaa ttcaaacagc
    23041 aataggctgt caaaactgaa taattacaac ttggatggcg aatttaaaag ctataaggtt
    23101 aaggataact tccgtcagat caacccggca gattacttcg gccatgaaaa tggttgctgc
    23161 cgccaagctc cgaaaggccc aggacaagat tgtcagactg aggccctatg caggcaagct
    23221 tcatgaaatc ctggttcacc ttacacaaag ccttgccgat gccgatataa aaaacattta
    23281 cagcaggcag gagtcacccg aaaaaatact gatcgttgca ataacctcaa ataggggact
    23341 ttgtggagca ttcaattcaa atgtcgttaa ggaggttaag cggctgatca cggaaacata
    23401 ccatgaacag tataaaaacg ggaatgttga attccttgca atcgggaaaa aaggctacga
    23461 ctcgctccga aaaatgaagc tgaacataat atcagaaaat aatgagcttc tgaatgattt
    23521 aacttttgaa aaagtttcgg cccttgccga gaacgtcatg aacgcatttg tcagcggtga
    23581 ctacgacagg gttgaactgg tttacaatca ttttaagaat gctgcaattc agcagcttac
    23641 gacagaggtt ttccttccgg ttgaaacaat ttccgaagag ggaaaggccg cggctcatgt
    23701 cgattatatc tatgagcctg acaagaaatc cattgtcagg gaactgatcc ccaaatccct
    23761 gaagatccag ttctataagg cagtcctcga ttcattcgtt gcagaaaacg gagccaggat
    23821 gacggcaatg cataaagcca ccgacaatgc cactgaaatg atccgcgacc tgacccttca
    23881 gtacaataag gcacgtcagg cagcgatcac gggtcagatt cttgaagttg taagcggagc
    23941 cgaggccctg aaataactga aaattattaa ttgaatccat gtagtgaaca tgtttgatca
    24001 atcggctctt aaggaaatcg tcaataacgc aataatcaat ctgtcctttg acatcgggtc
    24061 tgacaggctg attgatcctg ttaaatatgt tctctcgatg ggcggtaaac gcatgaggcc
    24121 ggtgctgtca cttatggcgt gcaatctttt cagtgacaag atcgaccatg ctgtcatacc
    24181 cgtgacggga attgagatat tccacaattt caccctggtc catgacgata taatggataa
    24241 cgccaggatc agaaggggat ttcccacggt acatacaaaa tggaacatca accaggctat
    24301 cctgtcgggt gacgttatgg cattcatcac aaacgaatgc tttcttcagt cacctgccca
    24361 tcttctgccc agggtattga aggtttttaa cagggccgcc attgaggtct gtgcgggtca
    24421 gcagcttgat atggatttcg aaagggctac atatgtgtct caggcagatt atatccggat
    24481 gataggactg aagacagccg ccctgattgc cgcctcatta aagatcggtg ccatttttgg
    24541 gggagccgat gacaaggatg ccggacacct gtacgaatat ggcaagaacc tggggcttgc
    24601 ctttcagata caggatgata tccttgatgt attcggcgac atgaaggttt ttggcaaaac
    24661 agccggcggt gatattattg aagggaaaaa aacattcctg tatgtcaggg caaatgaaac
    24721 cggatcgttt gatcagaaaa aaaagctgca ggatctttac gcctccaatg cattcaaacc
    24781 ggaggataaa atcaggatgg tcacggaatt atatgaactg cttgatgtta aatccagttc
    24841 tgagaagatt gtcgcagatt attttaaaga tgccctggaa catctcggga aagtgaatat
    24901 ttcacgtgaa aggaaggaag ggctcattgg gctgacttct tcacttgccg ggagagatca
    24961 gtgagtaaaa cgaaatgacg aattaatgaa ttggcaaata atttttaaaa ctcaataaat
    25021 aaatcggatt tatggcaaag aatattggga agatcagcca gattataggc ccggtggttg
    25081 acgtgacatt tgaacggcag ggcaatgaaa tgcctgatat ctatgatgct ctggaaataa
    25141 aaaggaacga cggtacgatc ctggttgtgg aatgccagca gcatataggt gaaaacacag
    25201 tcaggacaat agccatggac tcaactgacg gtcttcgcag aggtatggat gtggtggcaa
    25261 ccggacagcc gatcactatg cctatcgggg agcagattaa aggacgcctg atgaacgtta
    25321 ccggtgaggc aattgacgga ataggcgttc ttgataaaga gggcggctat cctattcacc
    25381 gcaagccccc gaaatttgaa gatctttcaa ctgaaaagga ggtgctttac acaggcatca
    25441 aggtaattga cctcattgaa ccgtactcaa agggcgggaa aattggtctt ttcgggggtg
    25501 ccggtgtggg aaagaccgtt gtgatccttg aattgataaa caatattgcc aaggcttatt
    25561 cggggctttc ggtctttgcc ggtgtcggtg aaagaacaag ggaagggaac gaccttttgc
    25621 gcgagatgct cgaagcagga gttatcactt acgggaagga atttctgaaa aacatggaag
    25681 ccggtggatg ggatctctca aaagtcgaca gggaagcgct aaaagattcg aagcttgccc
    25741 tggttttcgg acagatgaac gaaccgcccg gggcaagggc aagagtagcc ctgtcgggtc
    25801 tttcagttgc agaacatttc agggatggtg atgaaaaaag cggcggaagg gatattcttt
    25861 tctttatgga taatgtgttc cgctttaccc aggcaggttc tgaagtttcc gcgctccttg
    25921 gccgcatgcc ttcagcagtg ggataccagc cgactcttgc cactgagatg ggtatcatgc
    25981 aggaacgtat cacatcaacg cgccatggat cgataacgtc ggtacaggca gtgtatgttc
    26041 cggccgacga ccttaccgac cctgcgcccg ccacaacatt cgcccacctc gatgccacaa
    26101 ccgtgctgag ccggaaaata tcagaactcg ggatttaccc cgctgtcgat cctctcgact
    26161 caacatcgag gatactgaca cccgaaatag ttggcgaagc ccattacaat tgcgcccaga
    26221 gggttaagga actactgcag aggtataatg agcttcagga cattattgcc atcctgggaa
    26281 tggatgagct ttccgaagaa gacaaactcg tggttcaccg ggcccgaagg gtgcagagat
    26341 ttctgtcgca gcccttccac gttgccgaac agtttaccgg catcaaggga gccctcgttt
    26401 cgattgagga taccataaag ggattcaaca tgatcatgga cggaaaggtg gatcagtacc
    26461 ctgaatcggc attcctgctt gtcggaacta ttgaagatgc cattgaaaaa ggcgagaaaa
    26521 tacttgccca gtcgaaatga caaccgttga atgtcatatg ttgcagcctg cagcctgtta
    26581 ttgctgcttc ccgtgcatga agcctgcaac acactgtctt caacactcat gtcttactga
    26641 atcactatca tgaaaatcga gataataacc cccgatcaca cgatatatga aggggaaata
    26701 aaatccgtaa gggtgccggg caggaaaggt tctttccagg ttcttaagga ccatgcacca
    26761 atcatatcga ccctcgatcc cgggccggta tacatagttg aacaggataa cagggaggta
    26821 acctataata ttgacggagg cgttatagaa gtcagggcaa acaggatcat actcctgacc
    26881 gagtccgtca aataaaatat gcccgatgac agccgacgaa ttccggaaac acgcccatga
    26941 ggtggtcgaa tggatggcac gttacatgga aaacgtggag agctatcctg tcaaatccca
    27001 ggtcagacca ggcgagatcc ttgaaaggat ccctccagtg cctcctgcac agcccgaacc
    27061 tttcagcgcc ttcatgaagg atctcgatga gataatcatg ccgggaataa ctcactggca
    27121 gagtccgaac ttttttgcat atttcccggc caacacgagt cctccctcaa tacttgccga
    27181 gatgatcata tccacactcg gagcccagtg catgatatgg gaaacatcgc ccgcagcagc
    27241 tgaactggag gagaagatca tgatatggct gagggatctt atcgggctgc ccgctgaatt
    27301 cgagggtgtg atccaggata ccgcatcaac cgcaaccctg gctgctctga tcacggcaag
    27361 ggaaaaagca agcggcttca ggataaatga agaaggggca ggaagtacag cccggttaag
    27421 ggtctactgt tcggaacaga ctcattcctc tgttgagaaa gcggttaaga taagcggaat
    27481 aggcaggaac aacctggtta agatcccagt caaacctgat ttttcgatgg acgaggaaaa
    27541 actggagaaa gctgtttcgg aagacatgga aaaaggtttc attccatgtt gcgcctttgc
    27601 aacaattggc accacgggaa ccactgccgt tgacccttta agagccatcg gagagatctg
    27661 cagcagatac gatatctggc ttcatgttga cgctgcaatg ggcggtacgg cccttattct
    27721 gcccgaattt caatggatgc tcgatggcag ggaatatatc gattcatttg ttttcaatcc
    27781 tcataaatgg atgttcacca atttcgactg ttctgctttt ttcataaagc acccttcgct
    27841 tcttctgaaa acattcgaaa ttttgcccga atatctgaaa acccgcacca gggggctggt
    27901 aaatgattac cgcgactggg gcataccgat gggaagaaga ttcagggccc ttaagctctg
    27961 gagcgttata aggatgtacg gggttgaagg actccgggaa aaaatacggc gtcatattga
    28021 gtttgcagcg gaactggctg aaatgatagc aagggagccg gattttgaag tggcagctcc
    28081 ggtggtgatg aatgtcgtct gcttccgtta caggcccgca ggcaagtcag ccggggagat
    28141 caacggaata aatgagaccc ttaaccacag gctcaacgat accggcaaaa tctatctcac
    28201 ccatacagta atcaacggtc attataccct cagaatggtt acggggcaga caaatgtgac
    28261 catgaatcat gtgaggaagg cctgggagct gattaaaaca acagcggctc agctttaaag
    28321 attgtatgcc cttgtcctgt ttcttcctat actcattccg tatatatcca tgttgaacag
    28381 gagcctgatc acgggaaaag ttccaggctc acgccatgct gcctccactc ctgcagaaac
    28441 catgtacgga atccggaaaa taaaaaaatc accgagcaat tgtgctccaa acgatctgaa
    28501 agtcattttt ccttccgtaa aaaaccagat catgccgtta tccgtccgca taagcctgta
    28561 atggtcggtg acgtgcgtat agtcaaaaaa cagatctgcc cttatccttg tcagataaag
    28621 aaagcttgac agattaaagt cgggataaaa cagcggcatg tagtaatcag ctgagacaaa
    28681 atctgctctt ttcgagataa tattatcata tcctcttgaa aatgaaatta aattcttctg
    28741 caggaaccgt tcaggattct gagtttcctt ttcgagcctg acccttaaac catggttccc
    28801 ggataggccg gggaaataga aggcagtttt cagtgtggcc agatcaccat aaagctccct
    28861 gtcgaaggga aaagccgaat aactcagatc cattgtctgg gcccagcggg gatagatgtc
    28921 cctcaccgcg gtcctgtaat agttggaaaa atagatcctt ccggtaaacc ggctctggcc
    28981 cgggtcgtaa tacccgctgt ttttcatata aatatgatca ttgatatatt tccatgatgc
    29041 tgaaatacgc agatgttccg agaattttcc actctggaaa tacaaaggca gataaagggt
    29101 attcgtaatt gccattccct ggttgtattc agccggatcg ggaacatttt cgccgagttt
    29161 ttcaatcacg ggcctgtatc cgtaatcgat gaccgattcc agtgccggat accagccgag
    29221 ccagtttatc ctggcatgaa gcatgtgccg attatcagca tactcataac caatggtgga
    29281 aatgagggtg ctgaggttgt tctgcgtcat aagggttatt ccgggtttta taagggccgg
    29341 atcggatgtt atttcatcaa tgctggcata aaatggcatc cagctgtgaa atctgaaaag
    29401 gtttttccat tttttgtaag gttccggtaa aaataccctg ggctgacttt cccctgaact
    29461 gccatcatat ggtctgaacc tgttaataag ataggaagga ttctcactga aagccgcagt
    29521 atcaggctct tcactcctga gcgaagcctg gcaaatattg ttgccggaag ttgaatagtc
    29581 ggcaaacatt atattgttcc cgctgatatt cagatcgctt acaccgaatc gcgattttgt
    29641 gagcctgctg atttttccgt cgcctgccag cagatatacg ttatcggttc ccgatgccga
    29701 actgacaaaa aacaatgaat cgttcctggt aaaggcggat tgaatgtcat tggatgacgg
    29761 actgatatgg attttccatg catacttccg taaatcaaat gacataatac cttccccttc
    29821 ttcagaaagg gagacaaagg ttattatgtt gcccttctca tcccattggg gtttttgcag
    29881 ccacacaccc ggcggggccg ggatttcgcc gaggatgctt ccatcggctg cgtcaatgat
    29941 tacgagtccc gattcctgtg ttatggaact ccttaccgca gcaatgcggg aagcgtcggg
    30001 agatattgcc gcggccatgt acctggtctt cccggataac ttccgtgttg tccctgattt
    30061 cagatcaagt atttttatga ctgaccatgt cctgtttctc caccgcggat cggaatcaga
    30121 ctcaacccag acgatcttac ccctagcgta cgatatgttc cagggatagg aatagccggg
    30181 agtgtgtatc ctcctttccc ttccttcggc aggcctgatc agtacgaatg acgggggatg
    30241 gtcaagggat gtccttacgg caattatact atccttgccc gcccttactg gtgaataata
    30301 atttatgtac tttttccctt tcggcggatt gacaatgtca tattcaacac ttccgagggc
    30361 ggagtcttca gccgtccaca ggacctgcag ggtatcgaag gtttccctga acagcctttt
    30421 ttttgtcagg gatgcatttg ccctcaggct gaggttaaca gggttcaggg tgaaaggaag
    30481 gtttgcggta aggttaaggg cttttttcca gagggctgag tcatatttcg catacgacca
    30541 tgccatcatt tggtatcctg aatagtagtg atttggcaca aaatccctga acgatccgtt
    30601 tactatcttg tcatacttgt agattttccc cttttcaagc atcaatgctt tcagaggttt
    30661 ctggaatgaa ggggtccgtc cccttcccga gcctgttagc agggtttccg aaaaaacggc
    30721 atctccctcc aggaaccaca atggcagcag tgctgccacc gctccgttga attgctggcc
    30781 cgtaatgtac gacatcaccc tggtaaaacc cttatcgagc gatttcatct ggaaaacatg
    30841 ggtaagttcg tgtattgcca gctgggtttt cggatcgagc ggtattgaat tctgttcggg
    30901 tgtgggatat atctccatcc tgtatggtgc ccaggcgaca taaccattcg aggttgtggt
    30961 atgattatgg attatcaccg gtattctgaa cttcatttca gggtaaagaa gcatcagtcc
    31021 cgaatacgca tcttcgagtg ccctggtaaa ctcaattccc tggctgccgt agctttcagg
    31081 gtagatcacc ctgaacttat cagtttttat ttgcatccat tttatggacg acggttcctg
    31141 gcccgtctgg taatactggg cagcagccgg taccagggcc agggataaca acagcaacag
    31201 ggctgatctg gctgacttca tcaggaatca tttattcaaa aatagtaaaa tatggcggtg
    31261 agtctgcaaa aggccccggt tgggaaattt taattaattt cgttgtcagt tatgaatggc
    31321 atgatgccgt catcaaactc atcacactga taattgcatg aaaagttaca ggaaaatcaa
    31381 caatatcacc ggatgggtga tttttgccgt ttccgccgtc acctatctgt tgactcttga
    31441 acccacagcc agcctttggg actgcggtga attcattgca tcatcctata agctcgaaat
    31501 agggcatcct ccggggaatc ccgtattcat ggtgattgcc cggtttttca ccctgttcgc
    31561 cgggggaaat gtatcaactg ttgcgatcat ggtaaatgcc atgtctgccc ttgcaagtgc
    31621 atttacgatc ctgtttcttt tctggacgat aacccatctt gcaaggaagc tcattatcgg
    31681 gaacggggat agcactccgg gccggataat ggccgtaatg gccgctggtg ccacaggtgc
    31741 cctggcattc actttttccg attcattctg gttttcggct gttgagggag aagtatatgc
    31801 aacctcttcc tttttcaccg cagtggtatt ctgggcgatc ctgaaatggg aagatgttgc
    31861 ggatgagcct catgccgaca agtggatcat attaatagcc tacctcatgg ggctgtcgat
    31921 cggagtgcac ctgctcaacc ttctcaccct tcccggaatt gtcctggttt attatttcag
    31981 gaaatttgaa ttttcatgga aaggatttct tatctcactt ggcatatcag ccgtactgct
    32041 ttcattgctc atgtacggaa tcatgccggg gctggtcacc atatccgcga agctcgatat
    32101 cttttcccgg aacactctcg ggctgcctct caacagcgga ttgatattcc atttcatcct
    32161 gctgattgtc accttcattc ttgccgtaag gatgacgatc atctcaaatg acaggacaag
    32221 gatatccgtt ttcgccgcag cagccttttt tctgtcgggg atctgggtta tttcaggctc
    32281 tgcatttctc aacatagtcc tgctgggttc agtaatcttc ctggcatggt atctggccgg
    32341 aaggtacaag gtggtactaa acacgatact tactgcagtt atggtgatac tgataggtta
    32401 ttccgcaatt gccataatag ttatccgttc gtcagctcag ccgccgctga atgaaaatca
    32461 gccagacaat cctttcagcc tgctttattt cctcaacagg gaacagtacg gtcagaggcc
    32521 cctgttccgc ggtgcttatt acaatgcccc tgtcattgat tatgaggatg gaaagcccaa
    32581 gtattcaatt gaagacggaa agtacaaaat aactgcctac aacctggaaa ctatccatga
    32641 taccaggttc atcacactct tcccaaggat gtggagcgac cagcccgatc atgcgcgcgt
    32701 ttaccaggaa tggggaaaga tcaagggagt tccggttaca gtgaccgacc gctctggtga
    32761 aaagaaactg gtaagaaagc ccacgttccg cgaaaacctg aggtttatgt tcacctacca
    32821 gtttggctac atgtatttca ggtattttct ctggaacttt tcaggcaagc agaatgacac
    32881 acagggaaca gggggagcct tgaacggaaa ctggataacc ggcatcagat ttctcgacga
    32941 accccgggtc ggtacctccg acctgcctcc gggaatgaag gatgacacct cccggaacaa
    33001 gtactttctg cttcccttca tccttggggt ggtcggtatt ttttaccagc tgaaccgcga
    33061 catcaagaac tggagcatta ttcttctgtt gtttctgatg accggggttg caattgtgtt
    33121 ctatctgaac cagtatccca accagccgag ggaacgtgat tacgcatata ccggatcatt
    33181 ttacttttat gcggtctgga ttgggctggg agtgctggcg atctttgagg cagcaaggaa
    33241 gattgcggga gagaagacag ctgcgattgc ttgcggaata gtttgtctcc tggctgtacc
    33301 ggcggttatg gcctcggaga actggaagga ccatgacagg tcgggtcgtt acctggcccg
    33361 ggatgttgct tttaattacc tgaactcctg tgcaaaggat gccattcttt ttacaaatgg
    33421 cgataatgat acctttcccc tctggtatgc acaggaagtg gaaggagtga gaaccgacat
    33481 ccgggtttgc aatctgatgc ttcttaatac cgactggtat atcaaccaga tgaaatggaa
    33541 aaccaacgag tcggatcccc ttcccgtaag tcttccggcc cggaaatatt atgacggtgt
    33601 caacaatcag gtattcatag tcgaaaagac caaggatccc gtcgacataa aaaccgttat
    33661 cgattttgta aacagcgata acaagtccac caaggttcag gtttcagcga ccgagctgct
    33721 tgacataata cccgcacgca caataaggat acccgtcgac agggaaaaag tgctggcatc
    33781 cggaacggta aagcctgagg atgccgacaa aattgtcccc tggatagaca taaagcttac
    33841 caaaagctcg atactgaaaa gccagctgat tgtgatggat atacttgcca acaacaactg
    33901 ggagcgccca atctactatg taaccggtta tcatgatgat gccctgggcc tggaggagta
    33961 ttttcagtat gaaggactgg catacaggct tgtaccgata aagtcggagg ccaggagctg
    34021 gatcgattac ggaagggtcg atccggatat cctttatgat aatgtgatga acaaatttgt
    34081 atggggcggt gcgaataaca ggaaagtgaa tctcgattac aatcatatac ggacgcttat
    34141 ggtcgtaaag gcccggcttg tctatgcccg tcttgcaaaa gagctggcag caaggggcga
    34201 cagggagatg gccgccagtg tcattgatcg atgcatggag gttctgcccc ccgacaggtt
    34261 cccatatgat ccgtatttcc ctgaactcat tgaggctgcc ttaaaggccg gggcatcaga
    34321 caaagctgag gaaatgacaa gggcgtttac cgcttattac tacgcacgtc tcgattattt
    34381 ccttaagcag aagccgtata ttatcagttc ggctgagtat gagattcagt cagccattca
    34441 gtatgtctcc aaggttgccg gtttctgcat tgaaaacggc cagccagccc tgggagctga
    34501 aatacaggaa aggatcggaa aatacttctc agattattct tcagcggaag ggatagggat
    34561 agaataggat ctcaggagag attgatgact ttttccttaa catcctgctt cagatatttt
    34621 tcaagcaggg tgaatattgc ttcggggtac acgggtttaa gaatgtactc attgcatccg
    34681 gcaatgaaag catctgcctt cgactgttcc gatgaatatg ccgtgatcat cagtaccgga
    34741 acatgttttc tttctttcct gaatattttt gtgcagtcga tgccgttgat aagcgggatg
    34801 agaaaatcca tgatcacaag atcaatctgt gcatcatctt tttgtatgta atcgacaaaa
    34861 tccctgcctg tacgaagctt tactacattt gcacctgaac attgaatgag tgtttcataa
    34921 taccttagtg aaggcacatc atcctcaacg acaagaattg ttttgccttc caatgccaga
    34981 ttgctgaaat tcagggacat tacatgtttt gttttgacca cggtaaagct acaacaagat
    35041 acaaatgact aattaaatag agtgtgcaat tttcaacaaa ttatcaacaa atttaatgcc
    35101 gggaaatgag atttcccgga aatgaatgaa atatgcaggc agaattttcc ggaagcttac
    35161 ctgcattccc atgttatgcg ggtgttgccg ctgactatat cctcagtagc ttcgattgca
    35221 acaagttccg ctccgcagta ctccctttcc ctgccttcct gcaccaccgc tccgttctca
    35281 taagtgacct gcctgcagat cttgcacccg gtaagacctt cacatgatgt gagcgagaaa
    35341 accatcatta tgaacatcgg cactccgtag aaaatcctct ttctcatttt ttcccttttt
    35401 gatccggttt gatccctgag tcagttgatg aataaaattg ttcaatttta cgattttttc
    35461 agtaaattga tgcaacaatt gccgataaca ataatcttta ccggggaatg aaatcataat
    35521 ggacaatatt caataaataa tggaagccac aaaaatgaaa aagcatgtta cagttgtcgg
    35581 agcgatccat atcggttttg gaatacttgg actgatcggg gctctgggtc tgtttttcgc
    35641 acttagtttt gcaaagggag ttgttggtga cgaagaaata ccaagcatgt tgcttggttt
    35701 tctgtcgctc agcctcccca tcctgtcagg attcatggcc attgtcggta ttgccggagg
    35761 catcggactt ttgtcctttc aggtctgggc aagatacctg ataattgtga ttgcagcgct
    35821 tgaatgcctt ctgatacctg tcggaacgct gctgggcgtt tattcgatct gggtgcttct
    35881 ccaggatgag accatccggt tgttcactcc ggctaaaaca taacggctgc agaaggcttt
    35941 tggtcatcag aagcttaaga atccgagccg ttttctttca cttcaaagcc ttggaggccc
    36001 tgcatttatt caggaaatcc cgggtaatgc ccttttagtg atctgtagat catttgagcc
    36061 tgaacatcag tgaatcgagg atatatgatg tgaaaatccc caggcctccc ctgatatttg
    36121 aatacagcgg cttcgcttcc gagaaaggat tatcgctgcc attatattcc agcagcgatt
    36181 tatgatagag gtagtaggat tcttccgtgt tcatcacata aaccgtaatg aatgccgaat
    36241 cgtaatactg atatgaacgg gatattctgg tttccagttt tatgcggtta ttctgatctt
    36301 tccttacatc ctttaaaaat gattcctcaa accacaaccg ctctttatag acatctgtgt
    36361 caaatccttc gtatgtcttg tatcccgtga atctgcccgt gacactgtaa aaattgattt
    36421 cacccggatc atcggagaat tctgtcttta ttatgaattc cctccagtcg tcatagattc
    36481 cattgccttc atgaacaact gattccgtat ctatttcaat cataagatcc ctgagtcgtg
    36541 gaattgttgc tgatgcctct gcggtgagac ccttatcact cgtgacctta agggtatagg
    36601 ttttcccggg tatcagttcc atattcccgg gccggaaatg atatccctcg ccttctttta
    36661 tcaggggata ctcatataaa ccatcatcga ccgtaccggt tatgttcccg gggtcctccc
    36721 tgtggtcggc aaaatgacgg aggggctggt ttgatgttac ggatacaaaa taatcagtgt
    36781 cagcaggcga caggaacgac acgatcacaa gcttttgctc aaagtcaggc gagtgaaata
    36841 ttatatattt ttcacatccg gcaatcagta ccgggagaag gaaaaaagct gcatatttca
    36901 ttaacttgcc agtcatggtc agaacttaaa tgaatatgta aatgaaggga tgacggggaa
    36961 caggctcacc tgttcaacaa gggcaccact ggtcgtgccg gatgaaagat accggaaacg
    37021 gtaaaagaac gggttcttcc tgttatagac attgtaaacc gaaaattccc atatcctttc
    37081 tccccatttt ttttgcctgt gaaactgtat acccagatcg agcctgtggt atggtgccat
    37141 cctgtaatta tttttccggc tgtattcatt tacatcgtaa atattatgga aagggctgcc
    37201 cagtcctgat tctggtttca attccggaaa caggtcgaga tgatcgggag cataatagac
    37261 ggatgtgggc attgtaacag cattgccggt cccgtaaacc catgtccccg acagtgtgat
    37321 attctttttt atcctgtagg ttcccaccag cgagatgtca tgccgtctgt cgtaacgtgc
    37381 atagaatttc ttcccgtagt ttagggaatc gaactgcatc tgcgtccatg acagggtata
    37441 tccgatccat cccgaaaaac gacccgcctt tctgtgaacg aggaattcca gtccgtatga
    37501 ccatgccttt cccgcagtaa cattgtcttc ccagtttact tccctgatgt cacccatctc
    37561 atcaaactga aaaaacgatg cgccctcttt gtatccaagc acatgattca tggttttata
    37621 ataaccttct attgacaatg aaatgtcagg agttttcagg tccctgacag tcccgaaggc
    37681 aacctgccgc gactgctgcg gcccgaccct ttcggtggtc ggtacccaga ggtcggtagg
    37741 caggcttata ccggtgtatg agatcagatg gatatactga ttcattgaag catatgatgc
    37801 cttgagagca taatcgccat tgaacttcca tgttgcagat agccgcggtt caaggaacat
    37861 atattgcctg cttccggtaa gaaaatggct gaaccgcagg cccccgttca gcttcaggtc
    37921 gggcagcaac tgccatgtat cctctgcata caagcccgtt tcgagcccgt gtgatatctt
    37981 cttccggctc tttttcaggt ttatgggttc gtcgatttca atgaacacat agggagtgaa
    38041 cctgtggaaa atcatggcac ctcctgcttt taccgagtgc tttggcgaag gaagataatc
    38101 gagatcaaat ttaagtgtga ggtcccttat tcctgagaaa tactctgcac ggaaattctc
    38161 attttcaatc ctgctcttca gcttgtatgt gacaccgaat ttgtaatcgc tgaatacgaa
    38221 tgaagtattc gaaaacagcc ggctgctgaa aagatggttc cagcggaagg ttgcggtggc
    38281 attgccccag ccgagccctg cagtttcttt cagatcattc tcgtcatacc gggcatgaaa
    38341 tctgtctttg ccaaaatatc cgctcaggta tagtttgttt ctccttccga gatcataatt
    38401 tattttggca ttcagatcgt agaaataata acccgcatct gtttcctcca tggccctgat
    38461 tatgggtttc ataattaaat cgacatatgt tcttctgccc gaaaggagaa ttgaggattt
    38521 attcttttca agaggccctt ctagcgtcag tctcgatgaa atcagcccga tacctccctc
    38581 gccatgccat ttttccttat tcccctcctt catgttcatt tccaggaccg acgaaagacg
    38641 gccgccgtag cgtgccggga atccgccctt ggtaagctcc acactcttaa gggcatcgcc
    38701 gttgaatagc gagaagaagc caaagagatg cgatgcattg tagactatgg catcatcgag
    38761 aatgatgaga ttctgatcag ggccaccgcc tcttacatac aggccgctac tcccttcaga
    38821 acctttctgc accccgggca tcagctgaaa aactttgagc acatctttct caccaaggag
    38881 ggaaggaatg ttttttatct gggccaccgg cacgctaata gtgctcatac gagccgactg
    38941 gctgagtctt tctgcccttt cggccgatat ctcgacctca tcaagcaggg tacccggcct
    39001 caggtcgaaa ttcagttccg tatcctcccg caggctgatt ttttttgtct ggggggagaa
    39061 gcctacatag gatgcggtaa tttcaactga atctgcttcc gggagggtca gggagaaaaa
    39121 accataggtg ttggtcactg tcccggttct gtgatccgga aggtaaacat tcactcccgt
    39181 gagaagttca ccgcttcccg cttccctgac gaatccgctg accgtatggc gcaatgactg
    39241 ctgaccgctg atgatgtttc cgtgaaatat catgaaagcc agcgccaggt attgtaaggg
    39301 atggatgaat gtgttgtaca ggaccgactt cattatttta ccagaacagg gatataaata
    39361 tagcataatt aattcataaa cttaaaaatg cggctgcaga gcatccggcc ggaatttgcc
    39421 cgaagcactt gctgcctgtt ccattttgac cgtgacacat aaatcaattt ttcataaatt
    39481 gtcgtcaaaa ttctgactat gactgacgac aaaaagataa ttttttcaat gtaccgcgtt
    39541 gggaaaacct ttcccccgca ccgccaggtt cttaaggata tttctttatc gtttttcctt
    39601 ggagccaaga taggcatcat aggtttgaac ggatcaggca agtcaaccct gctcaaaata
    39661 attgccggga ttgagaagga gtaccagggt gaagtaacat tcctgcccgg gtattctgtc
    39721 ggtcatcttg cccaggaccc cctgctggat gaaaatcgca ctgtacgcga gatagttgaa
    39781 gagggaataa aggagattgc cgatctcgtc agggagtatg aggcaataaa cgaaaggttc
    39841 ggcgatcctg aagtttatga ggacccggcc aggatgcagc agcttatgga caggcaggct
    39901 gagctgcagg agaagattga ctataacgac ggatggaaca tcgatcataa gcttgagcgc
    39961 gcaatggatg cgctcagatg ccccgagggc agcgccaggg tgaatattct ctcgggaggt
    40021 gaaaggcggc gggttgcact ctgccgtctc cttctccgtc agcccgatat ccttttgctg
    40081 gacgagccca cgaatcatct tgatgccgaa tcggttcagt ggctggaaac aaccctgaaa
    40141 cagtatcacg gtactgttat ttgcataaca cacgaccgat attttctcga caatgttgcg
    40201 ggatggatac tggaactcga caggggcgag gggattccct ggaagggcaa ttattcctcc
    40261 tggctcgagc aaaaggcaca gaggcttgag attgaagaaa aagggaacat caaacgaagg
    40321 aagctgctgg agagggaact ggaatgggta cgaatgtcac cgaaagcacg ccaggccaaa
    40381 tccagggcca ggctcggagc ctacgaaaag ctgcttaacc aggatgtcaa ggcaaaggag
    40441 gagaaacttg agatattcat cccaaacggc ccgaggctgg gcgacaaggt tatagtagcg
    40501 aaaaaggtgg caaaatcatt tggtgaaaag ctgctttttg acgatctcgg gttcaatctg
    40561 cctcccaacg gcatagttgg agtcattggc ccgaacggag ccgggaaaac aacccttttc
    40621 aggatgataa tcgggcagga agaacccgac tcaggggttt tcgagatagg ggaaactgtc
    40681 gtcaccgggt atgtcgacca agcacacagc gagattgatc ccatgaaaac cgtatatgaa
    40741 gtgatctcag gcggtcagaa cgaagtacct gtcgggaaca ggcttgtaaa cgccagggca
    40801 tatgcggcca ggtttaactt cacgggggct gatcaggaaa agaaatgcgg cgtcctttca
    40861 gggggtgaac gcaacaggct tcacctggcc ctcaccctga aatcgggcgc aaatgtcctg
    40921 ctgctcgatg agcctacaaa cgatatagac gtcaatacat tgcgggccct ggaagagggc
    40981 ctggaaaatt ttgccggctg tgctgtgata atatcgcacg accgctggtt cctcgacagg
    41041 gtggcaacgc atatcctggc cttcgaaggt gaatcgaagg tagtctggtt cgagggaagc
    41101 tactccgaat atgaggaaaa ttacagaaaa cgggttgcgg ataccacgcc tgtaagaata
    41161 aagtataaaa agctggtgta gtaattggaa gaaagaggga gatctgtaaa cggcgaacct
    41221 tgaaatcacc ggcacaatca cctggcaggc agcccgggaa aattgatttt aattcactgt
    41281 cacatatccg gatgaagcga ctggcatttc ataaactgct tgtgtcattt gcaatcatgg
    41341 cattgccagc accatcgctt acagcacata taggcagtcg tttcccttcc gaaaagaaaa
    41401 cagttgagga taagattaca ggggcacagc tcacattcct taccagccag ggaccgggtg
    41461 atttcgggat ctgtaacacc ggccggcaat ggacggccga tggcaactgg atcattttcc
    41521 ggtcgaacag ggctcccggc gaaactcttg ccattaatga aaagaccggc gatatggtgc
    41581 aggttacaga ggggagctac atgggtgtga ctgctgtttc ggacaaatcg atgaagcttt
    41641 ttttcatgag gatgaccctt cgtcagccag gtcagtcagg cggcggaccc gttcagatcg
    41701 tggaggttga tcttgaaaga ctcctggccg acagcgaatc gggggatgtt agggatgaaa
    41761 gcgaatacca gaaggtacgt ggtacgatac ctgccgggat ggaagccggt ggaagcatga
    41821 gtatcgatgc cggcggggaa tggttgtatt tcagggtggg aagggctgaa gcttctaagc
    41881 atgccgcgca ggtcaatccg gcagaccggg gttactcaca acatcctgcc ggtgaaatcc
    41941 tttcaggcat cagcagcttc aacatcagga caagggagat aaggttcgtg ctgggactgc
    42001 cctttcgcgt tggccggatc agggccgatc agaaggtgtc cggggaactg tacttctgtg
    42061 gggaaaaaga cggtacgaat ccgctgcaaa cctggtcagt caggtcagat ggtacgggcc
    42121 tcaggaccgt ttgcggcggc ccgcagccga aatggcccgc cggttccggc agtgtcctga
    42181 tgcatcatcc ggcatcgtcc gacgacagac tggcctccgg gagcgatgcc gggggaaaca
    42241 tttacctggc cgacctgcat tcagacaaaa caatccttct ttccgccgga cacaaaacaa
    42301 cttcggctga tccaccatgc ccgtcagtca gccccgacga aacaaaagtc ctgatcctgt
    42361 cggcaatgct ttcttccgac acgcgccgtc tgaacctctg cattataaag attccggatg
    42421 gatggcaggg cagcaaacag acaggatatt gactgatgcg ttgcggatcg cgattcctgt
    42481 aagacaggat cccgcagtat atgattctaa agctggtgtt cgagtgtctc ctttgtcacc
    42541 tcgggagt
//
LOCUS       DAOOYG010000008        41458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_790_length_41458_cov_7.564718, whole genome shotgun sequence.
ACCESSION   DAOOYG010000008 DAOOYG010000000
VERSION     DAOOYG010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 41458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41458
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_41458_cov_7.564718"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..933
                     /locus_tag="PK288_01645"
     CDS             163..933
                     /locus_tag="PK288_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HPM17647.1"
                     /translation="MVRITKELKDFLDEKAARYNNPAFIGLDPVQVPHAYTGKEDIEI
                     SGFLSAVISWGNRASIVKNAKRMMGYMGDSPHDFILNHNDRHLRKMEGFVHRTFNSDD
                     LLAFIGGLRHLYKNMGGLEGIFVKYQTEDSLQPAIHYLKKEFFSVPHLERSRKHLPDP
                     LTGSAAKRINMYLRWMIRKDDKGVDFGIWKSIPPSVLSCPLDVHSGNVARKLGLITRK
                     QNDAKAVAELDTVLRGFDPEDPVKYDFALFGLGVFERY"
     gene            1398..2897
                     /locus_tag="PK288_01650"
     CDS             1398..2897
                     /locus_tag="PK288_01650"
                     /inference="COORDINATES: protein motif:HMM:NF040048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6298 domain-containing protein"
                     /protein_id="HPM17648.1"
                     /translation="MQKKIIILILLFTSYSFVLYSNSPESDANTMNRHFNGPLKTNPN
                     NPNYFIDNSGKAIYLTGSHTWANFQEIGIDTTKRFDYENYLKMMEECHHNFMRLWMFE
                     NSAFASWTPDTIFWHPNIYQRSGKGKAGDGGTKFDLTRFNQYYFDRLRQRIIQAGNRG
                     IYVSVMLFQGWSLDKTGISDPWRAHPWNKNNNINGIGARDSVADNNSLATIHSMRNPK
                     LLEIQEQYIKKVIETVNDLDNVLYEVSNEGGDHDWQEWVINLIREYEKSKNKQHAIGY
                     TTPSQLGHLNKNLYESSADWVSPTMEPLRWAYPGTKYMENYMDDPPANKFGKINILDT
                     DHLWGHGGNWKWVWKSFTRGYNPIFMDPWQPIEYHSDERVSWVFTGGVSKDNRDYPDW
                     HFVRTNMGYARKIADSVNLIDLFPEPDIASTGYCLTDKKNVFIVYLPNGGDVTINMLS
                     CDINANYYIEWFFPILNRSMKINTPVKGGGYKNFTLPFTGDGVLILIKN"
     gene            complement(3498..4157)
                     /locus_tag="PK288_01655"
     CDS             complement(3498..4157)
                     /locus_tag="PK288_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM17649.1"
                     /translation="MIRTSGITKSYGELKVLKGIDIEIRDREVVSIVGASGAGKTTLL
                     QIIGTLDRPDSGMVFYNGTDTNRLKGKALAAFRNTNIGFVFQFHQLLPEFTALENVCI
                     PAYIAGKGRAAAESRALEILGFLNLSERAGHKPSELSGGEQQRVAVARALINSPAVIL
                     ADEPSGNLDSENKNELHKLFFRLRDSFGQTIIIVTHDRQLAGMSDRILQIKDGLIVND
                     A"
     gene            complement(4230..4679)
                     /gene="tatA"
                     /locus_tag="PK288_01660"
     CDS             complement(4230..4679)
                     /gene="tatA"
                     /locus_tag="PK288_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPM17650.1"
                     /translation="MSGQEIFVIIVVALLLFGADKLPEIARTLSKGMRDFRKATDEIR
                     REFESSTADLRRDINEVAGSITRDVTEMSDSITRDVNEVKDNINKEVNEFTSDLSNDV
                     NEIKSSIEKDVNEVRENLEKDLKGESETTNQETTDQSTNPYTDTYTD"
     gene            complement(4740..7709)
                     /gene="secDF"
                     /locus_tag="PK288_01665"
     CDS             complement(4740..7709)
                     /gene="secDF"
                     /locus_tag="PK288_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HPM17651.1"
                     /translation="MQNKTAITILAIALALVCVYQLSFTFATYKVRNDAKAYAKGDLV
                     KQSAYLDSLSSLPKEDWNYFGYTFKEVQKKEMNLGLDLKGGMNVILEVSVEDILRGLA
                     NYSTDKTFNDALVRAKAEYQRQSQIDFLTLFGRAFEEIDPDAKLAAIFSTVGMRDKIN
                     FNSTNADVLRVLDAEISDAMKNAFNILSTRIDRFGVVQPNITQLSTKGRILIELPGQD
                     DPARVRELLQGTANLEFWETYENSEIIGYLSQANTLLREIQANTAKKDSVPVTSPAKP
                     VADTTGGEQSLLDLIKEDTATVTPATREEFNLQNPLFGILSPRVNEQGQPLPSCMVGL
                     AYGIDTAKVNRYLKMNQIRALFPRDLKLVWSQNPYKYDPKHSMYELHAIKVTTRDGRP
                     PLSGDVITSARPSSGITGSEVKVDFSMNAEGAKTWARMTRENIGRCIAVVLDGYVRSY
                     PRVQNEITGGNTEITGDFTIEEANDLANILKSGKMPAPARIVSDTIIGPTLGKEAINA
                     GLISFIISFIIVLGYMIFYYSKSAGAIADVALAGNIFLLLGVSASLGAIITLPGIAGI
                     ILTMGMSVDANILIYERVREELRKGKGIKLAIADGYKGAMSAILDSNITTLLTGIVLY
                     VFGTGPIKGFATTLVIGIFTSLFCSIYITRLIYERLLKKNTNLSFSIRLTANFLKDTR
                     IDFLGLRKIFYIASISVTIIMIISFFTRGLNAGIDFSGGRTFVVRFDEPVKTADVASR
                     LEEPFNEPPQVVTFGSENQVRITTKYKITESNVDDEIETALYEGLKDMVPAGVTKEEF
                     LSKYRVSSETIGPTIASDTKRRAVWAVGLSLVIMFFYILLRFKNWQYGLGAVVALAHD
                     SVIVIGLYSLFWGIMPFSMEIDQSFIAAILTIVGYSVNDTVVVFDRLREFLPLYRKRP
                     VKEVINLAMNDTLSRTINTGMTSILVLIVMFFFAGATIRGFLFAMLVGIIVGTYSSIF
                     IASSIVYDTTRKKGIKF"
     gene            complement(7997..9478)
                     /locus_tag="PK288_01670"
     CDS             complement(7997..9478)
                     /locus_tag="PK288_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM17652.1"
                     /translation="MKKVFFFLAAFYFLSPAPRSMAQELHRLTLEDVIKLSEEQSTQA
                     LMAKHRLRASYWQYRTFVAQYRPSLTLTGTTPNYSTAYERVWNSNTETWDYKSTNTLS
                     NFGSLSLSQNIGLTGGTIYLNSDLTLYNDFENETRKYITVPLSIGLNQPLFRYNDLKW
                     KKRTEPLRYEAARKTFLSNIENVHIAAVNFFFSLALAQINKEIAEMNYSNADTLYRIA
                     KGRYQLGTISEDELLQMELTWLNTETSRQEAAMNLRDREIRLRSFLGFNENVRLELIL
                     PSEVPSLQISPQEVLDLALANNPEMITQELAVLTAESNLAQAKGERGFNASLIASYGI
                     RDQDPDFLMAYHNTAKQQTARIGFTLPILDWGLGRGRYRMAKSSLELAKVQSQQAIID
                     FEQNLLLDVEQFNLQSSQVAVAAKSDTVAMKMFEVTKQRFLIGKIQILELNNADTKKD
                     QNKRAYVQALQNYWNYFYNLRNLALYDFLNRKPLETDYDKLIE"
     gene            complement(9489..10871)
                     /locus_tag="PK288_01675"
     CDS             complement(9489..10871)
                     /locus_tag="PK288_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM17653.1"
                     /translation="MFKFFFRYFHDIEIAVESIVSNKLKSMLTALGIIFGVAAVIAML
                     AIGKGAKQEIMEQMKMVGVNNILINPIIQEKTSSSEENGEKQQNKFSRGLSMMDVETI
                     RETIPSVKRISPEVSFSATAMMNGVKYTAKLVGVSNDYFELYNLPLIEGAFFNDFQAR
                     NGLQVCIIGANIRAKFFSKLNPIDQYIKFNGIWLKVIGVLQKTNVSLTGFEEKGVNVY
                     NDNIYIPIETMLLRFQNRALVNTKLVSAASTNPMMMFGGPGRMFGVSSSSDAGLSGET
                     NYNQLDRIVVQVNETEEITPTTEILSRMFTRRHSGVKDFEITVPELLLKQQQRTKDIF
                     NIVLGAIASISLIVGGIGIMNIMFASVMERIKEIGTRMAIGAKKMDIVVQFLSEAVLI
                     SISGGFVGIFLGVIMAKLIEEIAGIMTLVSFFSVFIAFGVSAAVGVIFGYSPAKRASE
                     RDPIESLRYE"
     gene            complement(10895..12463)
                     /locus_tag="PK288_01680"
     CDS             complement(10895..12463)
                     /locus_tag="PK288_01680"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPM17654.1"
                     /translation="MKRTIIITGIVVVLAIIIMIVIGRITKRKEVANLYVEAKRGQFD
                     IIVSTTGELEAENSEDIMGPDFRQSRSLRAMDIKITDMVPEGTEVKQGDYIATLDRTQ
                     FDNSLKDEREKLIQAEDNLEVKMLDTAVTLSGLRDNIKNLRYSVEEAQITLQESKYEP
                     PTTIRQAEIALDRAQRSLDQTIKTYGLKVEQAKTEMRTIRKNLSDQQQRVNDFETILA
                     KFVITAPSDGMVIYKRDRMNNKRKIGSSISPWDNVVATLPDMSTMISKTYVNEIDVSK
                     VKPGQKVELMVDAFPEKSYTGSVISVANIGEQLPNTDAKVFEVQIKVDGTDPILRPSM
                     TTGNKIITKTIDDVVYIPLESVQAGTDSIPFVYMKNGNRQIVLLGESNENNVIVEQGL
                     KEGDVLYLSTPAKPEGFRLTGGNLIAVNRERAKAKREEEERIRQEAAKSRQKMMPQPG
                     GPMSPEMMQNLRGNFQPGQNGRTGNGGPAGNPQNRAGSQQQPGMNPTASQRNLPERPV
                     GPGQNSPAGSRNPN"
     gene            complement(12641..14023)
                     /gene="glmM"
                     /locus_tag="PK288_01685"
     CDS             complement(12641..14023)
                     /gene="glmM"
                     /locus_tag="PK288_01685"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPM17655.1"
                     /translation="MALIKSISGIRGTIGGKPGEGLTPPDVVKFSAAYGTWLIRRNEG
                     RRLRVVVGRDARRSGPIVSSLVINTLRSLGIDIIDLGLAATPTVELAVTGTKSDGGII
                     VTASHNPAEWNALKLLDGNGIFLSADDGNEVLDIAAGEDFSFSAIDDTGTVVQDDTWN
                     RKHIEMILALELVDPEIIGKADFTVAIDCINSVGGIIVPDLLKALGVKKILELNTKAD
                     GFFAHNPEPLPHNLKDISELTAGGKADLGFVVDPDVDRLAIICEDGSMFGEEYTLVAV
                     SDYVLKHSPGNTVSNLSSTRALKDVTEKYGYRYYSAAVGEINVVEEMIKRGAVIGGEG
                     NGGVIYPELHYGRDALAGIALFLTHLARSGMKVSELRRLYPDYVITKRKIDLPGGTDF
                     TMILDRVREKFRGHQIDERDGLRIEHHEGWVQVRKSNTEPVIRIYAEGRSLEEADGLA
                     EEIIDIVKKS"
     gene            14364..15071
                     /locus_tag="PK288_01690"
                     /pseudo
     CDS             14364..15071
                     /locus_tag="PK288_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363300.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            15081..15959
                     /gene="era"
                     /locus_tag="PK288_01695"
     CDS             15081..15959
                     /gene="era"
                     /locus_tag="PK288_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPM17656.1"
                     /translation="MSHKSGFVNILGNPNVGKSTIMNALVGEKLSIITSKAQTTRHRI
                     MGIVNGDDFQIVYSDTPGIIKPRYKLQESMMRFVNAALTDADVIVFVTDVTEEKGDRN
                     VILEKIRNLHIPVIIVINKIDLGSQAGLEKLAAEWKNDFADSPVIGISAITKFNLNTL
                     LDTIISYLPECPPYFPKDQLTDKYERFFASEIIREKILLNYHREVPYSVEIEIESFRE
                     EEKLLRIKALIHVARVSQKGIIIGHRGERLKKTGTEARKDMEDFFGKKVFLEMFVKVS
                     RDWRDKPAELKRFGYR"
     gene            15979..17283
                     /gene="der"
                     /locus_tag="PK288_01700"
     CDS             15979..17283
                     /gene="der"
                     /locus_tag="PK288_01700"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPM17657.1"
                     /translation="MSGIVAIVGRPNVGKSTLFNRLTESRNAIVDSESGVTRDRIYGQ
                     AVWNGVGFSVIDTGGYVKNSDDIFEGEINKQVLIAIDEADIILMVTDVTAGVHDLDTS
                     VASLLRKSGRKVLLVVNKVDNNERLIDATEFYRLGLGDYFAVSSINGSGTGDLLDAIV
                     AGLPGKVQEEMPDIPRVAIAGRPNVGKSSLLNTLLGEERNIVTPLPGTTRDSIYTRYK
                     KYNHDFLLVDTAGLRRKGKITEDIEFYSVMRAVRTIENSDICLVMIDATRGPEAQDLN
                     IISLVTRNNKGLIVLVNKWDLIEKDNNTMKRYESEIRERTLPFTDYPILFISAINKQR
                     IHNILELVGRVNENRKRKIPTAELNEIMLGITRNNPPPAVKGKQVKIKYVTQLPTYSP
                     SFAFFCNLPQYVKEPYKRFLENRLREKYDFSGVPLRIFFRKK"
     gene            17331..17861
                     /locus_tag="PK288_01705"
     CDS             17331..17861
                     /locus_tag="PK288_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17658.1"
                     /translation="MKINRNIVFIISVLVAYSCIKIQNLPPEPYIEYTSFRVFDTIDT
                     LGNNVKGGELKFYFEDGDGDLGYLKSPEQEEGDSDESDLFFSLLRVNNGIVSPAPADD
                     PLRPSNYRIPYMERQGQNKILKGTISVIFMYLFYSPEDTIKYEFYVKDRAGNVSNTAT
                     TGTIPLYYDGLYTGSD"
     gene            complement(17863..21018)
                     /locus_tag="PK288_01710"
     CDS             complement(17863..21018)
                     /locus_tag="PK288_01710"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPM17659.1"
                     /translation="MRKTLFSTLVCIVFITGLSSGQVFRFRNFGSDNNLPSRVIYTIS
                     QSPDGYLWVGTTEGLARFDGFRFYPVVFPDSVSGRYPTASFTDSRGDTWFGCNDGTVF
                     YLKKRSLRQVSLSNSSAVSAFFEDQGGFVYAVAQRKSVFRINPGKIDEVKEIHVMSDP
                     VMLSACLDPSGELLLGTQENLLVCRITADSILIRDRIEGFDYSSIVTVNKLKGTQDFI
                     IGTDGNGIFHLTKSAGGEYSISRFSGYPELEMLTVKNAMTDTDGNMWLSTSGSGAVKL
                     VFSGPDGSMPGVQFFSRSSGLAGDNVSVIFQDAEKNIWLGFNGDGLSMLNNEAFQFFA
                     PGKERDPKSIIHIGRSGSNYLLGTPAGFYTFNPPDGEAGPFVPFTGLSGKVEIASFFE
                     DDENNIWIGTKGDGLYKRNSAGKTDRFFRSGDSGSDYIANICVAGENIWLSTLNGVII
                     VSKRTGTIKKTYNINTGLPHNSINQILVADDGSGYVATESDRLYRIDPDEGISSSGVP
                     MYGRLLNKIISLARDKENNIWAATLGNGVFRCTPDSVQGISTSNGLLSNYAYSILSDR
                     EDQVWIGHERGFSVYSPGIGTTRTYTTDFAKGGSCNPNAIYESADGKIFIGTTEGLVM
                     YDRSKDRKKETAPVNNINYIVINDVEYQYQPVFSFPYRKRYNIRINYTGISFSNPEEV
                     YYSTFMKNYDDDWSPVSRTREASYSLRDGKYTFNLVSVNEDGLGQSDPLSFEINIKPP
                     FWRAWWFVLSVTALVAGILIVIVRQREKAQKKIQEYLENELAARTRVVIKQKEEIELQ
                     NLEITDSINYARRIQSSILPDINKLREHFREAFIILQPRDIVSGDFYWFDKFDDERFI
                     LVCADSTGHGVPGAFMSMIGSTLLQDIVLRQRISRPSEILAMLDRQVFSTLNQNLETG
                     VSNDGMDIVVCEFSLKNKYIRFASAMRPVIIVMGGEICYIKGNRSSVGGQSVLEKYFD
                     DHEYYLNEGDTIYMFSDGLPDQFGGPEGKKMKIARLKNLLGEIWSLPVKEQEERLLKF
                     YHDWKGDLDQVDDILFMGVKI"
     gene            complement(21046..22047)
                     /locus_tag="PK288_01715"
     CDS             complement(21046..22047)
                     /locus_tag="PK288_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17660.1"
                     /translation="MKVNTAIFRILILSILSVTVLLSDASSQAVPGKDENIPFLVTFG
                     KDGETSWGDDDFFSIFFFTIPKDFSGQFYIKVFDPDVGGENDELQGVWNSKTMFSVYG
                     GKGVDPEKNIESRGLLPGVNYKTGNLLASRVFGDDARYDNKYYAFGPFNPSEGDFNEK
                     WNSYIFKIVCEGISGDDGNLYRYFLSRDANSDLPLEGANAFTYKYTFRMWNDTKSISH
                     IYPYIDTGIVSIKQKNFDWDDDGVILVVSTYKQGISVPVSGEDNWAESKIPVEQAEIG
                     KSLDFQFHKKKESLVRNNNVVISLENQRGDALQFFSSPIGGVPVYQPVIGIRKVQKK"
     gene            complement(22076..23509)
                     /locus_tag="PK288_01720"
     CDS             complement(22076..23509)
                     /locus_tag="PK288_01720"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM17661.1"
                     /translation="MIKRLRLILLVLFPGLLIVNSTAQELNSYEITRLPFSSSGYNDI
                     SPVMYNDGLLFCSDRRLSGIMERTSFDGRRLFNIYFVQRRDSVEWNKPKEIRSERSEL
                     FNNGPLSIAPDGKTVYFTSEIETGGQSKNKKFRNRSGIFIADLNGFQITSIRPFKYNN
                     PDYNIGQPSVSSDGKYLFFSSDMPGGYGGSDIYYCELINNEWSDPVNAGPMVNSTYIE
                     NYPYLHSGGRLYFTSDRPGGAGGLDVYYSEKNDREWGEPVRLPEPVNSASDDFALVAM
                     PDLESGYFASNRQRNDDIYEFIITIIRKATCDTLQENTYCYEFIEENAVKYDTIPFRY
                     EWRFGDGDTASGEIVQHCYAGPGSYLVQLDVVNLVTGEKSLNEKSERLVIQDIEQPFI
                     TSPDTAYTGQEIQLSADKTNLPGWQIAKYYWNFDDETAAIGQNVKKTFTRPGEYNIQL
                     IVSDKAGSGGKIKETCVSRNILIFNRP"
     gene            complement(23511..24470)
                     /locus_tag="PK288_01725"
     CDS             complement(23511..24470)
                     /locus_tag="PK288_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPM17662.1"
                     /translation="MVRTGLNIVVFLFVFCIAAGQSVHDSSRINLGFPVHSQYLQNGL
                     ILNPAYAGTRGVLSSLLSYRIQWIGTKGSPMIQSVSLHSPMKNDKVALGMTAQFMQYG
                     ATKATSIYADYAYHIKLRKGKLSFGLNAGADISKTDYASLLLNDPGDPVFKSNDRTHV
                     MPNVGAGAYYAEDRYFAGLSIPSFLNYRRSSNGSTVPYHSFGQYEFRLTAGSLIPVSD
                     LLKLKPSFLLNYSLSNSKKVSQFDINANFIIADIIWAGASWRISEQVAVGILQFQINN
                     QLMAGFSYDYPYGRMNTYTKGSTEFILRYEFRYKVSAANPRYF"
     gene            complement(24521..28177)
                     /locus_tag="PK288_01730"
     CDS             complement(24521..28177)
                     /locus_tag="PK288_01730"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM17663.1"
                     /translation="MSTLSGSVFQVLNGQEVIGGIVNSYAKVTSIGPGFVIVTDPAQA
                     AQFSPNDYVLLIQMQGVGIQTVQGSYGENVQSVFGTPGGYEFLVVQSVNIVTGRIDFT
                     RNTYVSINGYSTNSNLQLIRVPFYKSPEVASTVTCQPWDPSTGTGGVVAFLAGGKLTL
                     NADIDVTGRGFSGAQGSAGIGECVFTNESANNHDSYPLSWNNAGLKGEGISVHDYTGA
                     LLYPNHAKGQGRNFSGGGGGNGRYSGGGGGSNRGKGGDGGLEKYIPGQCGDDPRDGGF
                     GGMGITGTAVEDGIFAGGGGGASTQSAGSTASAGGNGGGIVIIIADTIDARNHFIRAS
                     GATAQNAVSDAGAGGGGAGGSIALSFQNLFSFLGLAAAGGNGGTNPGGFGQGGGGGGG
                     LIWLSPATLPAAVSSANVAGGTSGAPLAGGDPGEIRANYFPALNGFLFNSIRTAATNN
                     MIDSVCSDTNYGLIIGSQPVGGTPPYTFEWQSSVTSPSGGFTTAPGNSSQKNYTPPSR
                     LTATTWFRRVVRDYGATITDISMPVMVEVHPAIRNNIIGDPGILCYGQIPDILHSVQP
                     LQDGNGKYEFIWESSTDNITYTGTGADDESYQAPSGLTQTTWYRRRVFSGACVSTSDP
                     VRISVLPGISNNTISSDPQEICFGENFTNLQGSVAPILGGGDNNYIFRWERSADGATW
                     VEADGSNNDPDYNPSESAPYFPGQQYFRRVVLSGANNVCVNESAPVLLSSYPPVTNNT
                     ILPFGQTICAGTIPALLAGSLPLNGKGPGTYIYTWQDSTRGRTWRNIDEFTNVTSPDY
                     APPALTDSTFFRRIVHSSVCTSYSEAVSINVHSPVRENNLFFMEEGPSDTIICAGSLP
                     NRLIGSQPSGGTEQAGDFTYQWSSSPDNRNWTDIQASGTSRDYQPQTLTTTTYFRRRA
                     TSGMCSSESGPLLISVLPVISNNSISEDQVVCMADVPGILGQTQGMTLSGGSGVYTFL
                     WEESTDGINWTPAAGTNNNHDGSYQPPVMTGDIRYRRNVASGKNKCCQSVSNIVELVM
                     DKLPDDYIANAGKDTVIFSLDNIFSLSAGPAVEGGSGKWTVLQGSGSFQNDTFEKTEV
                     SGLSPGLNRFLWTVTRGACTVSDIVEINVNELFIPEGFSPNNDPEGYNNTFEIKGLDL
                     DNQIVELTIVNSAGSGVFSASNRNGSEWHAWDGKNSRGRDLPEGTYYYLLRITSTRTS
                     QLFKKSGFVILKRY"
     gene            complement(28290..32000)
                     /locus_tag="PK288_01735"
     CDS             complement(28290..32000)
                     /locus_tag="PK288_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM17664.1"
                     /translation="MKHPRFKYSLAILTAVMIILSIAVDSVYFSDFEYWLRTRKFNKV
                     LREKEKVMDECLNGMEPILAAGESHGSSSEKKLFSLAEKNRITLLEYIGNKLIYWSDN
                     DFDVPGFLVDSALLRPLIFVQNGWFLARSIQAGNERIVGLLRLRNDYGFENDIIKSGF
                     LRDFGITGEVGFSTDKTASEYHVVSADGTFLFSLVYPSEKDGNWYFKIVPMLMWTISF
                     LLFIFLVIRLTEYLVSHKRGTLALFSGPLIFLSVYVIILLTSYPAILTQFGLFSPYSY
                     TMNGFIPSLGHLLVLSVLMAATAFLFFRHPHFVDDFVNKPRQSWLRLTLLLVVAAFLF
                     LGYHLIFSHLILNSNINFETYKVLELNSLSLMGFITVSFLFSVPFLYLLKVLLASKDW
                     PAGSRIIPCVLSLSVFLIIAGNRPFEAISIAAFYIMVIAGIRLTSLFHAGTFNKSVIF
                     SVLFALYSLYIVTSNSERKTEEKINIQLLSYSTENDPTAEHLLLDMWPVIEADSVLAG
                     IMNVEYFEKDDVEMIAEYMRGKYFQGYWGNFNISFIVCTGNDSLRIGETGEIYDDCFR
                     FFNDRILRRGNRLTGSEFYYIDSRQGRANYLGRLFFNYGKGRINGLFIDIYSDINVFQ
                     PGYSELLLDKKYHSYARLRDYSFAKYINGELVLRTGDYPYNKNDTDYLISDSGYRAFV
                     EDSFRHLLYRNGNVTVIISRPALSFKDLIISFAYLFAFIFVFSNLLFVIIGKPVFMRP
                     AGMNFRQKLQMSFIAILLVSFTLVGYIIASLAISRYQDKHYENVKEKLNSVYIELENS
                     LMTERLQAERISDNSYYSLDEILVRLSNIFNTDINLYDLTGNLLATSRPEIFFRNLIS
                     RRMNNIAFMNLANLTKSEYFQNERIGNLYYISAYMPFYNHDNEIVFYLNLPYFRLQSV
                     LARDISDMIVTVMNFTLLLIVAAMSLAVFISGRLTAPLTVLSSRLASVELGKKSEHLL
                     YKGNDEVGDLVRQYNRMVDELEESARKLAVSEREYAWREMARQIAHEIKNPLTPMKLN
                     VQQLLKSWKDGVPGFEKKLERFTRNQIDFIDNLSSIAGAFSSFARLPEPKPSKVDLVE
                     SIRTTLELFKNTGNISFRVNWTHGRKILIFADREHVNSIFSNLIKNAIQAIPSEREGV
                     IRLNMEVEHQKVIVSVADNGTGVPESVQGKMFTPNFTTKSSGAGLGLSIVKRYIESAG
                     GRIWFKSEPEKGTIFYMEFPLFIDDQTPVNQE"
     gene            complement(32019..32492)
                     /locus_tag="PK288_01740"
     CDS             complement(32019..32492)
                     /locus_tag="PK288_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPM17665.1"
                     /translation="MKIVLLQTGKTTEEYIDEGVGNYSARIRKFTPFEIVTLPELKNT
                     RYMPVKEQKDREGAKMLQYLQKEDYIIVLDENGQEYTTHEFAGKLEKLFMLQKKRIVF
                     VIGGAYGLAGDVLDRADLRLSLSKMTFSHQVVRLLFAEQLYRAFTVIKGEPYHHD"
     gene            32673..33077
                     /locus_tag="PK288_01745"
     CDS             32673..33077
                     /locus_tag="PK288_01745"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HPM17666.1"
                     /translation="MKLIRLINVTAAFIITLGISAQEQARIPAGISIAIKSGNATELS
                     KYMNSTVELLLLQKEDFYKRNVAETILRDFFNEYHAKDFVIRHQGARNDAQYAIGNLD
                     TEKGAFRVYFLLKKVGGELLIHQIRIESDNGK"
     gene            33067..33909
                     /gene="nadC"
                     /locus_tag="PK288_01750"
     CDS             33067..33909
                     /gene="nadC"
                     /locus_tag="PK288_01750"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPM17667.1"
                     /translation="MVNDNLRKIILRSLEEDIGDGDHSSRACIPSRAKGKARLLIKEK
                     GILAGCRVALEIFSIVDGDLSAEVLIKDGSPMAPGDIVFYIEGRKRSILKSERLVLNI
                     MQRMSGIATRTREYADRLSGLKTKVLDTRKTTPGLRFLEKEAVRIGGGLNHRMGLYDM
                     ILLKDNHIDYAGGIEKAISRTIDYLNSTGKKLKIEIEARNLEDVRRIIKAGGVDRIML
                     DNFSLGETKAAVELIGGKFETESSGGITLENLRDYAECGVDFISVGALTHQVRSLDMS
                     LKAV"
     gene            34197..35498
                     /locus_tag="PK288_01755"
     CDS             34197..35498
                     /locus_tag="PK288_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17668.1"
                     /translation="MRKWIFFPVFIILFSCKGKVPETYLSHEQAAAYFARIEEICNRD
                     NGKLWGSNLFGPMMFVDRTSRQILANQPDKDGILKLKDGIYTGIYPREKLINITATEF
                     GGTLFGVAPLPPVEDEYRIITRAIHCLFHRFQYLNGIYPAIFNSTNMDEREARIWLKL
                     EWKALRKAITSEGEDKLTAIRDALIFRGTSQISFPGYVDEGNHFETYEGLTSFTFTFL
                     PSASMDEFKKRLFEYLDRIYSFQSYSQSYGGIHGALYATLLYQKGFDFKNLISDKTDL
                     GKLVMELYNIELPEICRDVAGSLAISYNLDQIYREEEQRLRDIRERLCKQVNIFNQKP
                     VVFIGLESPYFDFEPEDVHSLDTLGTLYSHIRVSDNWGKLTVNKHGCLISGNYKQLRI
                     TAKGLKADKNHIYGEGWHMILNEDWVIDEVNDNYYVHRIAP"
     gene            complement(35536..38163)
                     /locus_tag="PK288_01760"
     CDS             complement(35536..38163)
                     /locus_tag="PK288_01760"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPM17669.1"
                     /translation="MDIPSKYEPAKAEDKWYSYWMRHGFFRSIPDDREPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDILVRRARMLGKNACWIPGTDHASIATEAKVVAKLRAEGIEK
                     RNLTREEFLKHAWEWTEKHGGIILEQLKKLGASCDWDRTLFTMDEKRYDSVIKVFVDL
                     YNKGLIYRGVRMVNWDPEAMTAVSDEEVYYTEEKSKLYYVRYKISGEDGYVMIATTRP
                     ETILGDTAVCANPSDERYLHLKGKKVIVPMTGREVPFIFDDYVDMEFGTGCLKITPAH
                     DINDYEIGIRHRLPSIDIFNDDGTISGRAGKFEGTDRFTARKLAEQELKKTGSLVKVV
                     SYNNKIGRSERTDSVIEPKLSTQWFLKMKNISRPALDAVLNGDIHLYPPKFRNLYRHW
                     MENVHDWCISRQLWWGHRIPAWYYGDGETVVAENAEEALNLARKQSGNNSLAPDDLKQ
                     DEDVLDTWFSSWLWPITSFDGINDPGNPDLRYYYPTNDLITAPEILFFWVARMIIAGY
                     EFRGEKPFRNVYLTGLVRDQQRRKMSKSLGNSPEPLDLIKKFGADGVRVGMMLCSPAG
                     NDLLYDDSLPEQGRNFANKIWNAFRLVKSWKTDDRIPQPESSAITVRWMNEILRKSVG
                     EIDANFRKFRISEALMSTYRLFWDEFSGWYLEIIKPEYEKPVDKITYDATIEIFDSLL
                     HLIHPFMPFITEEIWQLLSPRKDGESLMISRMPSSRKHNKELITRFAYARDIVSAIRT
                     VRKELQILNRDPVRLLVRSGREAFDGEFLPVIKKLANISEIEFISDKQNGSVSFIVGT
                     TEFYIPVAGSINIENERNRLKADLEYYRGFLSSVMKKLDNERFVQNAPARVLELERKK
                     KADAELKIRSLEESLEALN"
     gene            38348..39550
                     /locus_tag="PK288_01765"
     CDS             38348..39550
                     /locus_tag="PK288_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPM17670.1"
                     /translation="MPSLSEKGILIPPSPIRKLVPFSEEAKRKGKKVYHLNIGQPDIS
                     TPAIALDALRNLDLRVIEYGHSAGNESYRQKLAEYYKKAGINIDHTQMLITTGASEAI
                     LFAFFACINPGEEIITPEPFYANYSGFAVTAGITITSVTSTIENDFALPPVSEIERKI
                     SPKTKGILLCNPNNPTGYLYSEKELQMIRDIVSRHDLFLFSDEAYREFCYDGMKHISA
                     MNLDGIGDNVVLIDSISKRYSMCGVRIGALISRNSEVISSALKFAQSRLSPPGLGQIA
                     GEASIDTPDEYFLEVNREYTMRRDFMVNALNKIPGVYCPKPMGAFYTVVKLPVDDSEK
                     FAQWILEEFDYKNQTVMIAPASGFYSTPGLGWNEARIAYVLNVEDLKNAVETLEQALK
                     IYPGRTIQ"
     gene            39551..40279
                     /locus_tag="PK288_01770"
     CDS             39551..40279
                     /locus_tag="PK288_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPM17671.1"
                     /translation="MNNVLRCIAIDSDPLVLDMIGEFCTKVYYLDLTGKYTDPFRAYQ
                     ILNENKVDLMFLDVRMPQITGAEFLNSLYNPPMVIFTSSFKEHALEGFECDAVDYLVK
                     PFAFDRFFKAVNKAFQLQKLKKPVDYLQDENSQPAQRFLMVKVEYSTIRVDLREILYI
                     EGLKDYVKIHCGSRLLLTKTTMKNILEKLPQEIFFRVHKSYIVAVEKIDMIENSRIII
                     GNQRIPIGESFRSSFFSLVSKNLV"
     gene            complement(40284..>41458)
                     /locus_tag="PK288_01775"
     CDS             complement(40284..>41458)
                     /locus_tag="PK288_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17672.1"
                     /translation="HMYNANSQSSSSPYSLFGLGSLETSSLGPGKGMGGTSIAFRSAT
                     YLNISNPASFSGLDSLVSIFEIGLFGKYTSYASFTKNQSSIDANFRYVVMGFRITPWL
                     ATSFGFAPYSTIGYNINTKSDVEGSNLKYSKVFSGDGGINRTFLSTSFKITKNLALGV
                     NASWLFGNITHRELSETYYYTLEDVTYLSNFHLNYGLNYRFAINENNFSVGLIFGDSK
                     KLNAKNTTTIKTNTETEVIRGRSSKYSIPRNFGAGLAYERGFFQAGIDFERSLWEGTE
                     FKNSGIRSRNCDRISAGVEFPSFGPNKGTGRMLLYRVGAEYRESYFIINNTPIDYYAL
                     SFGTGIPLKGVLSTVNVSVELGQNGVKKKDLFRENFVTVHLDLSLRDLWFIKRRYL"
BASE COUNT        11166 a         9607 c         9004 g        11681 t
ORIGIN      
        1 atttttccgg atgcttcgca tctgaccttc gcggaaagaa caatttcccc cttatttaaa
       61 ggatcggcgg caaaaaagat aaaaatattc tttcggataa ttaaggtagc gtaggaaacc
      121 cttgtcatta tcagtatttt tgacctgttt aaagaaatat gcatggtacg aatcacaaaa
      181 gaactgaagg attttcttga tgaaaaggcg gccaggtaca acaatccggc attcatcggg
      241 ctcgaccctg ttcaggtacc ccatgcatat accgggaagg aagacattga aatatcgggg
      301 ttcctgtccg cggtcatatc atggggaaac cgtgcctcca ttgtaaagaa tgcaaaacgg
      361 atgatgggat acatgggaga ttcgccccac gacttcatcc ttaaccataa tgaccggcac
      421 ctcagaaaga tggaaggttt cgtgcaccgc accttcaatt cagatgatct cctggcattc
      481 attggaggtc tgaggcacct gtataaaaac atgggcggac tggaaggtat attcgtgaaa
      541 taccagacgg aagattcact ccagccggct attcactatc tgaagaaaga gtttttttcc
      601 gtaccccatc tcgaacggtc gaggaagcat ctgcccgacc cgctgacagg ttctgccgct
      661 aaaaggataa acatgtatct caggtggatg atccgcaagg atgacaaggg cgtggatttc
      721 ggtatctgga aaagcattcc gccctcggta ttgtcatgtc cgcttgatgt tcattcgggg
      781 aacgttgccc ggaagctcgg actgatcacc cgtaaacaga atgatgccaa ggcagttgcc
      841 gagcttgata ctgttcttag aggctttgat ccggaagatc ccgtaaaata tgattttgca
      901 ttgtttggac tgggagtgtt tgaaagatac taatagaaaa atgatcaaat tttcagtctt
      961 cttaccccga ctgggcaact caatgataac atattattca gcgaacgctt tcaagctcca
     1021 tattttctct accaagccgt aatatatcag ccagcgattc tcgccctgat aatctatcaa
     1081 ctttgatagc agctcatctg gatttttctt cataatttgc tcacgttctt tgaaagtcat
     1141 ggttgtaagt tttttgatta gtactttaga actattattc aaccgtgact ttctttttct
     1201 attacttcgc taatccaatt ttacagaaac aaagctacgt agcgtgtaac tttttgatat
     1261 cattaatttg ttcaatttta gattgtagtt ttaggctata ttatatcctg taagttaaac
     1321 ttgactaaaa ggattttggc atttataggt agatattttt atattacaga aaatttttca
     1381 atttaatttt tcatattatg caaaaaaaaa taatcatcct aatccttcta ttcacttcct
     1441 attcttttgt tttgtattca aatagccctg aatcagatgc gaatacaatg aatagacatt
     1501 tcaacggccc attgaaaaca aaccctaata atcctaatta ttttattgat aattcaggaa
     1561 aagccatata cctgacagga tcccatacat gggctaattt tcaagaaatt ggtattgata
     1621 caacaaaaag atttgattat gaaaattatt tgaaaatgat ggaagaatgc catcataatt
     1681 ttatgaggtt atggatgttt gagaattccg cttttgcaag ttggacgcct gatacaatct
     1741 tttggcatcc aaatatttat caacgttcag gaaaaggaaa agctggagac ggaggaacta
     1801 aatttgattt aaccaggttt aatcaatatt attttgacag attacgtcaa cgtattatac
     1861 aggctggcaa tcgaggcatc tatgtttcag tgatgttatt tcaaggttgg agtctggata
     1921 aaactggcat ttctgatcca tggagagcac atccatggaa taaaaataat aatattaatg
     1981 gcattggagc cagggatagt gttgccgata acaactcatt agcaacaatt cacagcatga
     2041 gaaatcctaa attattggaa attcaagaac aatatattaa aaaggttatt gaaacggtaa
     2101 atgatcttga taacgtttta tatgaagtta gcaatgaggg cggtgatcat gattggcagg
     2161 aatgggttat aaatttgata cgtgagtatg aaaaaagtaa aaataaacaa catgcaattg
     2221 gttatacaac cccgagtcag cttggtcatt taaataaaaa tttgtatgaa agcagtgcag
     2281 attgggtgtc gccaacgatg gagccgttaa ggtgggcgta tccgggaaca aaatatatgg
     2341 aaaattacat ggatgaccct ccagctaata aatttggtaa aatcaatatt ctggatacgg
     2401 accacttgtg ggggcatggc ggtaattgga aatgggtatg gaaaagtttc accagaggat
     2461 ataatccaat ttttatggat ccttggcagc caattgagta tcacagcgat gagcgagttt
     2521 catgggtttt cacgggtgga gttagtaaag acaatcgtga ttaccctgat tggcattttg
     2581 tgcgtactaa tatgggatat gccaggaaaa tagcagatag tgttaacttg atagatttgt
     2641 ttccggaacc ggacatcgct tcaaccggat attgtttgac ggataaaaag aatgtattta
     2701 tcgtttattt acctaatggg ggtgatgtta ctataaatat gctatcctgt gatataaatg
     2761 caaattatta tatcgagtgg ttttttccaa ttctcaacag gtctatgaaa attaatacac
     2821 cagtgaaagg aggcgggtat aaaaatttca ctttgccttt tactggtgat ggggtgttaa
     2881 ttttgattaa aaattaaatg taccatttac aatgtacagg tgactgtttt tgtttctgat
     2941 tatcccggat atataggagt acagctgctc cgagtgatcc cggacgaggg aaggaatact
     3001 gtgctttgtc ctgaaaacaa gatggtacag caattgccga tagcttgaca tagcattgat
     3061 gtttttgtta ataatacaac gagacgaaag caagatacta aattccaggg aaaatccggg
     3121 tctgtgtcgc cccgttcggg gctcaaaatc attggtgtga ctttaacccc cggttaacac
     3181 cgggggttat taatattcag tcacctacgt gactctcttg tccggatgct tcgcatctga
     3241 ccttcgcgga agctgatact tcccttcaga agatacctct cttctactgc catccaccgg
     3301 tatgaataat attgaagttg atagctttcc cccgtaacat aactctctgg caaagagatg
     3361 ctccgaagga gcatccgtca accggtttat taaacggaga gccggaggtt tggcatacat
     3421 tggcaaagtg aaagggatcc tgtgtttttc aaatgtcggt taacccgctg aggaggatga
     3481 gctgcatcct tttccgttca ggcatcattt acaatgagcc cgtccttaat ctgcagtatc
     3541 ctgtccgaca tgccggcaag ctgcctgtca tgagttacaa tgattattgt ctgtccgaat
     3601 gagtccctga gcctgaaaaa cagtttatgc agctcattct tgttctcgga atcgagattc
     3661 cccgagggtt catcggcaag gataactgcc gggctgttaa taagggccct tgcaacggct
     3721 acccgctgct gttcgccgcc actcagttcg gagggtttgt gccctgccct ttccgacagg
     3781 ttaaggaaac ccagtatctc gagcgcacgc gattcagctg cagccctgcc ttttccggca
     3841 atataggcgg ggatacagac attttcaagt gcagtgaatt ccggcaggag ctgatgaaac
     3901 tgaaaaacaa atccaatgtt ggtattcctg aatgcagcca gggcttttcc ttttaaacga
     3961 ttggtgtcgg tgccgttgta aaacaccatt cccgaatccg gacggtcaag ggtacctatt
     4021 atctgaagaa gagttgtttt tcctgctccg cttgccccga caatggaaac cacttcccta
     4081 tccctgatct ctatgtcgat ccccttaaga accttaagtt caccgtatga ttttgttata
     4141 cccgaggttc tgatcatttt gtagatgtgg gatgattttg caaatcagga ccggttagct
     4201 gaattcatga atgtatcttt tccgaaatac taatccgtat atgtgtcggt atatggattg
     4261 gtgctttgat ctgtcgtctc ctggtttgtg gtttccgatt cgcctttcag gtctttttca
     4321 agattttccc tgacctcatt cacgtctttt tctatactgc ttttaatttc gttcacatcg
     4381 ttgctgaggt cactggtaaa ttcattaact tccttgttta tattgtcttt cacctcattt
     4441 acatcccgtg tgatgctgtc cgacatttct gtgacatccc tggttatgga accggcaact
     4501 tcatttatat cccttctcag atcggctgta cttgattcaa attctctcct gatttcatcg
     4561 gtggctttcc tgaaatcacg catccctttc gaaagggtcc gcgctatttc aggcagcttg
     4621 tctgcaccaa aaagaagaag ggcaacaaca ataataacaa atatctcctg accgctcatc
     4681 tgccattctc ttctttaaat tcaacgatta cggcaaaagt aggaaaaata tcatgttttt
     4741 cagaatttaa ttcccttttt ccgggtcgta tcgtatacga tggaggaagc aatgaatatt
     4801 gaagaatatg ttcccacaat tataccaacc agcattgcaa aaaggaatcc cctgatggtt
     4861 gctccggcaa agaagaacat aacgatcaga accagtattg aagtcatgcc tgtattgatg
     4921 gtacggctca atgtatcatt cattgcgagg ttgataacct ctttcacagg ccgtttccgg
     4981 tatagcggaa ggaactccct cagcctgtcg aaaacaacaa cggtatcgtt aaccgagtaa
     5041 ccaacgatcg taaggatcgc ggcaatgaat gactggtcga tttccatcga gaacggcatg
     5101 attccccaga agagcgaata aaggcctata actattacgg aatcgtgggc aagggccaca
     5161 actgcgccta acccgtactg ccagtttttg aacctcagta agatataaaa gaacataata
     5221 accagtgaca gtccgacagc ccatacagcc cttctcttgg tatctgatgc aattgtcggt
     5281 ccgatcgttt cagagctgac ccggtatttg gatagaaact cctctttggt cacaccagcc
     5341 ggtaccatat ctttaagtcc ttcgtaaagc gctgtttcaa tctcgtcgtc gacatttgat
     5401 tctgtaatct tgtatttagt cgtgatccta acctgatttt cacttccgaa agtaactacc
     5461 tgcgggggtt cattaaaagg ttcttcaagg cggctggcca catccgcggt ctttaccggt
     5521 tcgtcgaaac gaactacaaa agtcctgcct cctgaaaaat ctatgcctgc attaagtcct
     5581 cttgtgaaaa atgatattat cattataatg gtaacggata ttgaggctat gtagaagatt
     5641 ttcctgagcc caaggaaatc gatccgggta tccttgagaa aatttgcggt cagcctgata
     5701 gaaaatgaca ggtttgtatt tttcttgaga aggcgctcat aaatcagccg tgtgatatag
     5761 attgagcaga acaatgaggt aaagatacct attaccagtg ttgtggcaaa tcctttgatg
     5821 ggacctgttc cgaaaacgta caatacaata cctgtaagca gtgttgtgat attcgagtcg
     5881 agaatagctg acatggcgcc tttgtatccg tcggcaattg ccagttttat ccctttcccc
     5941 tttctcagtt cctcgcggac cctttcataa atcaggatat ttgcatcgac tgacattccc
     6001 attgtaagaa ttatccctgc aatacccggg agtgtaataa ttgcaccaag agaagctgac
     6061 acaccaagga ggaggaatat gttcccggcg agggcaacgt cggcgatggc accggcgctc
     6121 ttgctgtagt agaatatcat gtacccgaga acgataatga aggatattat gaatgatatc
     6181 agtcctgcgt tgattgcttc ctttcccagg gtcggaccaa ttatggtatc ggagacgatc
     6241 cttgccggtg ccggcatttt ccctgattta aggatgtttg ccaggtcatt ggcttcctca
     6301 atggtaaaat cgcccgttat ttcagtgttg ccgccggtga tttcattctg cacccttgga
     6361 tatgatctca catagccatc caggaccacc gcgatgcagc ggccgatatt ttcccgtgtc
     6421 attcttgccc aggtcttggc gccttctgca ttcattgaaa aatccacctt aacctctgat
     6481 cccgtaatac ctgatgaagg tcttgccgaa gtaattacat cgccactgag tggcggtctg
     6541 ccatcgcggg ttgtcacctt gatggcgtgc agttcataca ttgaatgctt tggatcgtac
     6601 ttgtagggat tctggctcca taccagcttg aggtcgcgag ggaagagggc tctgatctga
     6661 ttcattttaa ggtacctgtt aacctttgcc gtatcaatcc cgtaagccag tccaaccata
     6721 catgaaggta atggctggcc ctgttcgttc actctcggac tcaggatccc aaacagagga
     6781 ttctgaagat tgaattcctc cctggttgca ggggttactg ttgcagtatc ctccttaata
     6841 aggtcaagca gcgattgttc cccgcctgtt gtatcagcta caggttttgc cggggaagtt
     6901 accggaacac tatccttttt tgcggtattg gcttgaatct ctctcagaag agtatttgcc
     6961 tgcgaaagat atccaatgat ctcactgttc tcatatgtct cccagaactc caggtttgcc
     7021 gttccctgga gcagttcccg cacccttgcc ggatcatcct gcccgggcag ctcaatcagt
     7081 atgcgtccct tggttgaaag ctgggtaatg ttgggctgta ccacaccaaa cctgtcgatc
     7141 ctggtactca ggatgttgaa cgcgtttttc atagcgtctg atatttcagc gtcaagcacc
     7201 ctcagaacat cagcatttgt cgaattgaaa ttgattttat ccctcattcc aaccgtgctg
     7261 aatattgcag caagctttgc gtcgggatct atttcctcaa atgcccttcc gaacagagtg
     7321 agaaaatcaa tctggctttg cctctgatat tcggcttttg ccctgaccag ggcatcgttg
     7381 aaagtcttgt cggttgaata gtttgcaagg cctctgagga tatcctccac tgaaacttcc
     7441 agtattacgt tcattccacc cttgaggtca agcccgaggt tcatttcttt cttctgcacc
     7501 tccttgaagg tatatccgaa atagttccag tcttctttgg gcagagacga aagggaatcc
     7561 agataagctg attgctttac cagatctccc tttgcatatg cctttgcatc atttctgact
     7621 ttataggtag caaaggtgaa agagagctgg taaacacata ccaaagccag tgcaattgcc
     7681 aggatcgtga tagctgtttt attttgcatt tctgtctttt ttgtatttta tcgatgattt
     7741 tccaagtccg accgattgag gcgcaaatat ataatttttt aagataaatc cgcagaccgg
     7801 atattaaaag aaaagattca aataagacgg ttgttaaaaa ttctttaacc gctatgtgaa
     7861 catatgttat aacccgttca tcaggccggg ccgatgtttt tgcatcccgg gcgggtgcgg
     7921 cattaatctt gtgatatctg cggttacaga tcccggcttt ttaagcattc cctgtgaacc
     7981 agatgaaaaa ggaattttat tcaatcagtt tatcgtaatc agtttcaagc ggttttctgt
     8041 taagaaagtc atacaatgcg aggtttctta aattgtagaa atagttccag taattctgca
     8101 acgcctgaac atatgccctt ttgttctggt ccttctttgt atcggcatta ttcagttcga
     8161 ggatctgtat ttttccgata aggaatctct gtttggtaac ttcgaacatt ttcatggcaa
     8221 ctgtatcgct tttggcagca acggccacct ggctcgactg gagattaaac tgttccacat
     8281 caagcaggag gttttgttca aaatcgatga tggcctgctg cgactgaact ttcgccagct
     8341 caaggctgga ttttgccatc ctgtagcggc cccggcccag gccccagtcg agtatcggca
     8401 gagtgaaacc tatcctggct gtctgctgct ttgcggtatt atgataagcc atcagaaaat
     8461 cagggtcctg atcacggata ccgtaagaag cgatcaggct tgcattgaat cccctctctc
     8521 cttttgcctg tgccaggttg ctttcggcag tcagcaccgc aagctcctgg gttatcattt
     8581 ccggattatt tgcaagtgcc aggtccagaa cctcttgcgg actgatctgc agggaaggaa
     8641 cctcagaagg caatatcagc tcaagcctta cattctcatt gaatccgagg aatgatctaa
     8701 gcctgatttc cctgtccctg agattcatgg ccgcttcctg cctggatgtt tcggtattga
     8761 gccaggtaag ttccatctga agcagttcat cttccgaaat ggtacccagc tgatatcttc
     8821 ccttggctat ccggtaaagt gtgtcggcat tggagtagtt catttcagca atctccttgt
     8881 ttatctgcgc aagtgcaagt gagaagaaaa aattgacggc agctatatga acgttctcaa
     8941 tatttgaaag gaaggtcttt cttgcagctt cgtacctcag gggttccgtc ctttttttcc
     9001 acttgaggtc gttgtatctg aacaacggct ggttcagacc gatgcttaac ggaacggtta
     9061 tatacttcct tgtctcgttt tcaaaatcat tgtaaagggt aagatcggaa ttgagataaa
     9121 tggtacctcc tgtcaatcct atattctgtg aaagtgaaag ggatccgaaa ttactaaggg
     9181 tattggtgga tttatagtcc caggtttcgg tattggagtt ccataccctt tcatacgcgg
     9241 tactgtaatt gggtgtggtt ccggtcaggg tgagtgatgg cctgtactgg gcaacgaagg
     9301 tcctgtattg ccagtaactg gccctgagcc ggtgtttggc cataagagcc tgggtggatt
     9361 gttcttcgga aagtttgatg acatcctcaa gggtcagcct gtgcagttcc tgcgccatgg
     9421 atcgcggagc aggggaaaga aaataaaatg ctgccaggaa aaaaaacact tttttcatta
     9481 tcaatacatt attcatatct caacgattcg atgggatccc tttcagatgc tctctttgca
     9541 ggtgagtacc cgaatatgac cccgacggca gccgatacgc caaaagcaat aaatacggag
     9601 aaaaatgaaa ccagggtcat tattcccgcg atttcctcaa tcagcttggc cattataact
     9661 cccaggaaaa taccgacaaa tccgcctgaa atacttatca gaacagcttc ggaaaggaat
     9721 tgaacgacaa tgtccatctt ctttgcacca atggccatcc tggtccctat ttcctttatt
     9781 ctctccatga cggaagcaaa cattatgttc atgattccta tgccccccac aataagcgat
     9841 atgctggcaa tggctcccag aactatattg aatatatcct tggtcctctg ctgctgtttc
     9901 agaagcaact ccgggactgt aatctcgaaa tccttgacgc ccgaatgccg tcgggtgaac
     9961 atacggctca ggatttctgt ggtaggagtg atctcttcgg tctcattcac ctggactact
    10021 atcctgtcga gctggttata gttggtttcc ccgcttaatc cggcatctga actgcttgaa
    10081 acgccgaaca ttcgcccggg tccgccaaac atcatcatcg gattggtact tgccgcggat
    10141 accagttttg tgttcacaag ggccctgttc tgaaacctca gcagcatggt ttcgatcggg
    10201 atgtaaatat tatcgttgta cacattcact cccttttcct cgaaacccgt gaggctcacg
    10261 tttgtcttct gcaatacccc tattactttc agccagatcc cgttaaactt aatatactgg
    10321 tcaatgggat ttaatttact gaaaaattta gccctgatat tggccccgat aatacagacc
    10381 tggaggccgt tcctggcctg gaaatcgttg aaaaaggcac cctctatgag cggaagattg
    10441 tagagttcaa aatagtcatt tgataccccc acaagctttg cagtatattt cacaccgttc
    10501 atcattgccg ttgcactgaa cgatacttca gggcttattc ttttaaccga gggtatggtt
    10561 tccctgatgg tctcgacatc catcatgctc agtccccgtg agaatttatt ctgctgcttt
    10621 tcaccgtttt cttcggaaga ggatgttttt tcctggatga ttggattaat caggatatta
    10681 tttactccaa ccattttcat ctgctccatt atttcctgct ttgctccttt ccctattgcg
    10741 agcattgcta tgacggcagc aacgccgaag ataatgccca gggcggtaag catcgactta
    10801 agcttgtttg atacaatcga ctccacggct atttcgatat cgtggaaata tctgaaaaag
    10861 aatttaaaca tgcccttaag gttttacgac atttttaatt tggattcctg ctccctgcag
    10921 ggctattctg acccggtccc acaggccgct ccggaagatt cctttgactt gccgtcgggt
    10981 tcattcccgg ctgctgttgt gatccggccc ggttctgtgg atttcctgca ggcccgccat
    11041 tccctgttct gccgttctga cccggctgga aattgcccct cagattctgc atcatttccg
    11101 ggctcatcgg accacctggc tgaggcatca ttttctgcct gctttttgcg gcctcctgcc
    11161 tgatcctttc ctcttcctcc cttttggcct ttgctctttc cctgttaaca gcgatgagat
    11221 tcccgcccgt tagcctgaaa ccctccggtt tcgccggcgt gctcagatac aatacgtcac
    11281 cctctttcag gccctgttcc acaatcacat tgttctcatt ggattctcca agaagaacta
    11341 tctgcctgtt cccgttcttc atatatacaa acgggatact gtcagtacct gcctgaacac
    11401 tttcaagggg aatgtaaacc acatcatcaa tggtcttggt aattatcttg ttccctgttg
    11461 tcatcgaggg tctcaggata ggatccgtac cgtcaacctt tatctgaact tcaaacacct
    11521 tggcatcggt atttggcagc tgctccccga tatttgccac acttatgacc gaccccgtat
    11581 aagacttttc gggaaatgca tctaccatca gttcaacttt ctgacccggt tttaccttgc
    11641 ttacatctat ttcatttaca tacgtttttg agatcattgt tgacatgtcg ggaagagtgg
    11701 caacaacatt atcccagggg gaaatggaag aacctatctt tctcttgttg ttcatcctgt
    11761 cccttttgta aatgaccatc ccatcagaag gtgcagtaat cacaaacttg gcaagtattg
    11821 tttcaaagtc attcactctc tgctgctggt cagagagatt ttttctgatg gtcctcattt
    11881 ctgtttttgc ctgttcgacc ttgagaccat aggttttaat tgtctggtcc aaagagcgct
    11941 gggccctgtc aagagctatt tcagcctgcc tgatggttgt cggcggttcg tatttggatt
    12001 cctggagtgt tatctgcgcc tcctcaacgg aatacctgag attctttata ttatcccgta
    12061 acccgctcag cgtgacagca gtatcaagca ttttcacctc gaggttatct tccgcctgga
    12121 tcagtttctc cctctcatcc ttaagggaat tatcgaactg ggttctgtct agggtggcaa
    12181 tataatctcc ctgtttcacc tcggtcccct cgggaaccat atcagtgatc ttgatgtcca
    12241 tggccctcag gctcctgctc tgcctgaagt caggacccat aatgtcttct gaattctctg
    12301 cttccagttc tcccgttgtt gaaacaatga tatcaaactg acctctcttt gcttcgacat
    12361 agagatttgc cacttctttc cttttggtta ttcttcctat tacaatcatt atgattatag
    12421 caagaactac aacgataccc gtaataataa ttgttctttt catccgaatt atgctgattt
    12481 aaattttcct attgtcatta tgaaccggca aaattaccta aaccaattca cccggccgac
    12541 attttaacaa catttaataa atcaatgttc aacgtcttgc gactaaaaga cattttaagc
    12601 cggtcattta ttccacggaa ccaaagaata agattttata ttatgatttt ttaacaatat
    12661 caattatctc ctcagcaaga ccatcagcct cttcaaggga ccttccctcg gcataaatcc
    12721 ttatgacagg ttcggtattt gatttgcgta cctgcaccca tccttcatga tgttcaatcc
    12781 gaaggccgtc tctttcgtca atctggtgtc cgcggaattt ttcccggacc ctgtcaagta
    12841 tcatagtgaa atcagttccg ccggggaggt caattttcct tttggtaatt acataatcgg
    12901 gatacaaacg cctcagttca ctgactttca tcccgctccg ggccagatgg gttaggaaaa
    12961 gagcgattcc cgccagggca tccctgccat aatgcagttc gggataaatg acccctccgt
    13021 taccctctcc gcctatcacg gctccccttt ttatcatttc ctcaacaaca ttaatctcgc
    13081 caaccgcagc tgaataatac cggtatccgt atttttcggt tacatccttc aaagcccttg
    13141 ttgaagaaag attggataca gtatttcccg gactgtgttt aaggacatag tccgatactg
    13201 caaccagtgt atattcttct ccgaacattg aaccatcctc gcaaattatt gccagcctgt
    13261 caacgtcggg gtcaacgacg aagccaaggt cagcctttcc cccggctgtc agttccgaga
    13321 tgtctttaag gttgtgcggg agaggttccg gattatgcgc aaagaaaccg tcagcttttg
    13381 tgttcagttc caggatcttt ttcacgccca gggctttcag gagatccggt acaataatgc
    13441 cgccgaccga attgatgcag tcaatcgcaa ccgtgaagtc tgcttttcct atgatttcag
    13501 gatcaacaag ctcaagtgcc aggatcattt caatatgttt cctgttccat gtatcatcct
    13561 gaacaacagt acctgtatca tcgattgccg aaaaactgaa gtcctcaccg gcagcaatat
    13621 ccaatacttc gtttccgtca tcagcagaaa ggaagatccc gtttccgtca agcagcttga
    13681 gcgcgttcca ttcggccggg ttatgactgg ccgttacaat tatcccgccg tctgattttg
    13741 tccctgttac ggcaagctcg acggtaggtg tggctgcaag tcccagatcg atgatatcga
    13801 taccgagact cctcagggta ttaataacaa gactgcttac tatcgggccc gaacgccggg
    13861 catcccgtcc gacaacaacc ctgaggcgac ggccttcatt gcgccgtatc agccatgtgc
    13921 catatgctgc agaaaatttc acaacatcag gaggcgtgag tccttcaccc ggtttgcctc
    13981 ctatggttcc cctaattccg gaaatagatt ttatcagcgc catattgtat tgaatttaag
    14041 actgcaaagg tgcaaaaaaa atcctaatga attccccgta ttcaatcatt gattaaagtt
    14101 taatgaagat cggacaaaat ggtatgatac tgaaaagcac ccggcagaac agtgtatccc
    14161 taaaacaatg atcataacta agtgcatggc atttaacaga ctttaaaaca tctaatacgc
    14221 aggaattcgc agcttaggtt ttttaatcgt atctttgccg ccacagaaaa ttcaatgtaa
    14281 tgggttacat tcagcccggg aagaaagaca attcagggaa aaggttacgt gattcagcgg
    14341 cttcacaaaa gggagttgtt ctgatcaggc ttaacagatt cattgccaac agcggtgtct
    14401 gttccaggag ggaagctgat tccctgatca gggggggaca cataacgatc aacggcaagg
    14461 ttgttaccga catgggtgta aaggtttcag tgcatgacat tataaagtac aaaggcaaaa
    14521 ggctctcatc ggagaaaaag gtttatatcc ttctgaacaa accagccggg tacgtaacca
    14581 ccgtcaggga tccccacgct gaaaatacag tgctggacct gattcgcgga tgttgtcctg
    14641 aaagggttta tcccgtggga aggctcgata aggccacaac cggagtactg cttcttacaa
    14701 atgacggcga gctggcaggg aagcttaccc atcccagtca taaaaggaaa aagatctacc
    14761 atgtttttct cgacaggccg gtgataaaaa atgatctgct gcggctcgca catggaattg
    14821 aacttgacgg tgaaattata tcagctgatg ctgtttcata tgccgatcct gatgacagga
    14881 cccaactggg aattgagctt cattcaggcc agaacagggt tgtaagaagg ctcttcgagt
    14941 caatgggata caaagtcaga aaactcgaca gggtatactt tgcaggattg accaagaaga
    15001 atctgcccag ggggaaatgg cgattccttt cagaaaagga aataagcatg ctgaaaaggg
    15061 gattttctta attttcaggg atgagtcaca aatcgggttt tgtaaatatt ctgggaaatc
    15121 ccaatgtagg gaagtcgaca ataatgaatg cactcgtggg tgaaaagctt tcaatcatta
    15181 catcgaaggc acagaccacg aggcacagga tcatggggat tgtcaacggt gatgattttc
    15241 aaatagttta ttcagacacc cccggcatca taaaacccag gtacaagctg caggaatcaa
    15301 tgatgaggtt tgtcaacgca gctctcactg atgccgatgt gatcgtcttt gtgactgatg
    15361 tcaccgagga gaagggagac aggaatgtaa tcctggagaa gatcaggaac ctgcatatac
    15421 cggtaattat tgtcatcaac aagatagatc ttggcagcca ggccggcctg gaaaaacttg
    15481 cagccgaatg gaagaacgat tttgcggatt cgccggtaat cggcatctcc gcgatcacca
    15541 aatttaatct caacactttg ctggatacca taatttcata tctgccggaa tgtcctcctt
    15601 acttcccgaa agatcagctc accgacaaat acgagagatt tttcgcatcg gaaataatcc
    15661 gcgaaaagat attgctgaac tatcacaggg aggttcctta ctctgtggaa attgaaatag
    15721 aaagcttcag ggaagaagaa aagctcctga ggataaaggc cctgatacat gttgcacgtg
    15781 taagccagaa agggataatc atcgggcacc gtggtgaaag gctgaaaaaa acaggtaccg
    15841 aagcccggaa ggatatggag gatttcttcg gtaaaaaggt attccttgaa atgtttgtaa
    15901 aagtcagccg ggactggcgc gacaaacctg cggaacttaa aagatttggt tacaggtgat
    15961 taataattat acgtacagat gagcggaatt gttgcgatag ttggcaggcc gaatgttgga
    16021 aaatccaccc tgttcaacag gcttacagaa tcgcgtaatg ccattgttga cagtgaaagc
    16081 ggagtaacac gcgaccggat atacggacag gcggtctgga acggcgtcgg tttctcggtt
    16141 attgacaccg gcggatacgt gaaaaattcc gatgacatat ttgagggaga gatcaacaag
    16201 caggttctta ttgccattga tgaagccgac ataatactga tggtgaccga tgttacggcc
    16261 ggagtgcatg atctggatac ttccgttgcc agccttttaa gaaagtcagg caggaaagtc
    16321 ctccttgtcg taaacaaggt tgacaacaac gaaaggctga tcgacgctac tgaattttac
    16381 aggttgggcc tgggtgatta ctttgccgtc agttcaataa acggaagcgg aacgggcgat
    16441 ctgctcgatg caattgtggc aggccttcct ggaaaggtac aggaagaaat gcctgatatt
    16501 cccagggttg ccattgcagg gcgtcccaat gtagggaaat catcgcttct taacacactg
    16561 ctcggggagg agaggaatat tgttaccccc ttgcccggaa caacacgtga ttcaatatac
    16621 acaagatata aaaaatacaa tcatgacttt ctgctggttg acaccgcagg tttacgcagg
    16681 aaaggtaaaa taactgaaga tattgaattt tattctgtca tgagggcagt caggacaatt
    16741 gagaattctg atatctgcct tgtaatgata gacgccaccc gcgggccgga ggcccaggac
    16801 ctgaacatca tttcacttgt tacaaggaac aacaagggtc tgattgttct tgtgaacaaa
    16861 tgggatctta tagaaaaaga caacaacacc atgaaaaggt atgaaagtga aatacgcgaa
    16921 agaaccttac cttttaccga ttatcccatt ctgtttatct ccgccataaa caagcagcgt
    16981 atacataaca ttcttgaact cgtcggcagg gtgaatgaga acaggaagag aaagatcccc
    17041 acagcggagc tgaatgagat catgctcggg attacccgga acaatccccc gccagcggtc
    17101 aaaggcaaac aggtaaaaat caaatatgtt acccagcttc ccacatattc gccctcgttt
    17161 gcatttttct gcaatctccc gcaatacgta aaggaaccct acaagaggtt cctggaaaac
    17221 cggctgcggg agaaatatga tttttcgggt gtcccgctca gaatcttttt cagaaaaaaa
    17281 taacttactt tggtgtggtt tttgtgcaag actgatttaa accggtagag atgaaaatca
    17341 accgcaacat agtttttatt atttccgtcc tggtcgctta ttcctgcatc aagatacaga
    17401 atcttccgcc cgagccctat attgagtata ccagcttcag ggttttcgat accatcgata
    17461 cactgggcaa caacgtcaag ggaggtgagc tgaagtttta ctttgaagac ggcgacggcg
    17521 atctcggata tcttaagtct ccggagcagg aggaaggcga ttccgatgaa tcagatcttt
    17581 tcttttccct tctgagggtt aacaacggca ttgtcagtcc cgcacctgca gatgatcccc
    17641 tccgcccttc caattacagg attccctata tggaaagaca gggtcagaat aaaatactga
    17701 agggtacgat ctcggttatt ttcatgtatc tgttttattc cccggaagac accatcaaat
    17761 atgaatttta tgtaaaggac agggcaggta atgtaagcaa tactgccaca accggtacca
    17821 ttccgcttta ttatgatggt ttatatacgg gatcggatta gttcagatct tcacccccat
    17881 aaataaaata tcgtccacct gatccaggtc acccttccag tcatgataaa atttcaggag
    17941 tctttcttcc tgttccttaa cgggaagtga ccatatttca cccagcaggt ttttcaggcg
    18001 ggctattttc atctttttac cctcgggccc gccaaactga tccggaagac cgtcagaaaa
    18061 catatatatt gtgtcgcctt cattaagata atattcatgg tcgtcaaaat acttctccag
    18121 aactgactgc ccccctaccg acgacctgtt tcccttgatg taacagatct cgcctcccat
    18181 tacgattatc acgggcctca tggcagaagc aaacctgatg tacttgtttt taagtgagaa
    18241 ttcacaaacc actatgtcca ttccatcgtt ggagacacct gtttcaaggt tctgattgag
    18301 tgttgaaaag acctgcctgt cgagcatagc caggatttcc gatggcctgg atatcctttg
    18361 ccgcagtact atatcctgga gaagggtaga gccgatcatc gacatgaagg cacccggcac
    18421 tccatgcccg gttgagtcgg cacataccag gataaacctc tcatcatcaa acttgtcgaa
    18481 ccagtaaaaa tcaccgctga ctatgtcgcg cggctgcagg ataataaagg cttccctgaa
    18541 atgttccctc agtttattta tatcaggaag gatgcttgac tggatcctcc ttgcataatt
    18601 gatactgtcg gttatttcca gattctgcag ttctatctct tccttctgct ttatgacaac
    18661 ccgggtcctg gctgccagtt cattctcaag gtattcctgg atctttttct gggccttctc
    18721 cctctggcgg acaatgacga tcaatattcc cgcaaccagc gcggtaactg acaaaacaaa
    18781 ccaccaggcc ctccagaaag gcggttttat gttgatctca aaggaaaggg ggtcactctg
    18841 accaagcccg tcttcattca ctgacaccag attgaatgta tatttaccat ccctgagact
    18901 gtatgaagct tcccttgtcc tggatacagg cgaccagtcg tcgtcatagt tcttcatgaa
    18961 cgtactgtaa taaacttcct cgggatttga gaagctgatg cctgtatagt ttatcctgat
    19021 attgtacctt ttcctgtatg gaaatgaaaa aacaggctga tactgatatt ccacatcatt
    19081 gataacaata tagttgatat tgtttaccgg tgcggtttcc tttttccggt ccttcgacct
    19141 gtcgtacatc acaaggcctt cggtcgtacc aatgaatatt ttcccatcag ccgattcata
    19201 tattgcattc ggattgcagg aaccgccttt tgcaaaatcg gtggtatagg ttcttgtcgt
    19261 ccctataccc ggtgaatata ccgaaaagcc cctttcatgt ccgatccaca cctggtcttc
    19321 gcggtcggaa agtatgctgt aagcgtaatt tgacaatagt ccgtttgagg tactgatacc
    19381 ctgcaccgaa tcgggtgtac atctgaacac tccgttacca agagtagcag cccagatatt
    19441 gttctcctta tccctggcca gggaaatgat cttgttcaga agtcttccgt acattggtac
    19501 tcctgatgaa ctgattcctt catcgggatc aatcctgtaa agcctgtcgc tctcggttgc
    19561 aacataaccg ctgccgtcat ccgcaacaag gatctggttt atactgttat gcggaagacc
    19621 tgtatttata ttgtaggttt ttttaattgt tcccgttctt tttgaaacaa tgatcactcc
    19681 gttcagggtc gacagccata tattttcacc tgcgacacag atattggcaa tgtaatcgga
    19741 tcctgaatcg cccgaccgga agaacctgtc tgttttacct gctgaatttc gtttgtaaag
    19801 tccatctccc ttggttccta tccatatgtt gttttcatca tcctcaaaga atgatgcgat
    19861 ctcaacctta cccgacagcc ctgtaaacgg aacaaagggt ccggcttctc cgtcaggcgg
    19921 gttgaaagta tagaatcctg caggtgttcc cagaagataa ttgctgcccg atctccctat
    19981 gtggattatg cttttgggat ctctttcttt accgggtgcg aaaaactgaa aagcttcgtt
    20041 gttcagcatt gacaaaccat cgccgttgaa gcccagccat atattttttt cggcgtcctg
    20101 aaagataacg cttacattat cgccggcaag tcctgacgat ctggaaaaaa actgcactcc
    20161 gggcattgat ccgtcagggc cggaaaatac aagttttacg gctccggatc cggaggttga
    20221 taaccacata ttgccatctg tgtcagtcat tgcatttttt accgtcagca tttcaagctc
    20281 gggataaccg ctgaaacgtg atattgaata ttcccctcct gcagatttag tgaggtggaa
    20341 tattccgttg ccgtcagttc ctatgatgaa atcctgagtt cctttcaatt tgtttactgt
    20401 cacaatgctt gaataatcaa atccttcaat cctgtccctg atcagtattg aatcagccgt
    20461 tatacggcat accaggagat tttcctgtgt tcccagcaga agctcaccgg agggatccag
    20521 gcaggcggaa agcattacag gatcagacat gacatgaatt tccttcacct catcgatttt
    20581 cccgggattt atcctgaaca ccgatttcct ttgcgcaaca gcataaacga atccgccctg
    20641 atcctcaaaa aaagcactta ctgcagaaga atttgacaaa ctgacctgcc gaagagatct
    20701 ttttttcaga taaaaaaccg ttccgtcatt gcagccaaac caggtatctc ccctgctgtc
    20761 ggtgaatgaa gctgtgggat accgtcctga cactgaatcg ggaaagacga caggataaaa
    20821 acggaatccg tcaaaccggg caaggccttc tgtagtgcca acccacaggt aaccgtcagg
    20881 gctctggctg atggtatata tcacccggct tggaagattg ttatctgatc caaagtttct
    20941 gaacctgaaa acctggccgg aagataaacc cgtaataaag actatgcaca ccagggtgct
    21001 gaacaaggtt ttgcgcataa taaaaagcta agtaaattat ttcccctatt tcttctggac
    21061 ttttcttatt ccgataacgg gctggtaaac cgggacgccg ccgatgggag agctgaagaa
    21121 ctgaagggca tcacctctct gattttcaag ggatataacc acattattgt tcctgaccag
    21181 gctttctttc ttcttgtgaa actggaaatc aagcgatttg ccaatttctg cctgttcaac
    21241 ggggatcttt gattctgccc agttgtcttc gccggaaaca ggtactgaga tcccttgctt
    21301 gtatgtggaa acaacaagta tgactccatc atcatcccag tcgaaatttt tctgttttat
    21361 tgacacgatc ccggtatcga tatacggata aatatgtgag atgctctttg tatcattcca
    21421 catcctgaaa gtatacttgt atgtgaatgc attggcacct tccagcggaa gatcgctgtt
    21481 tgcatcccga ctcagaaaat atctgtaaag gtttccgtca tcgccgctga tcccttcgca
    21541 tactatcttg aaaatataac tgttccactt ttcattgaaa tcgccttccg aagggttgaa
    21601 gggtccaaac gcatagtact tgttgtcgta tctggcatca tctccgaata cccttgaagc
    21661 aagaagattc ccggtcttat agttcactcc ggggagaaga ccccttgact caatgttttt
    21721 ttcaggatcc acaccttttc cgccatacac ggaaaacatg gtttttgaat tccatactcc
    21781 ctgtagttca tcgttttcac caccaacatc aggatcaaag accttaatat aaaactgtcc
    21841 gctgaaatct ttggggatgg taaagaaaaa gatggagaaa aaatcatcat ctccccatga
    21901 ggtttctccg tctttcccga aggtgacaag aaacgggata ttctcatcct tccccggaac
    21961 tgcctgacta gaagcatcag acaataagac tgttacggat agaatagata aaatcagaat
    22021 cctgaatatt gcagtattca ctttcattga tccgcaaaaa taattatttt ataattcatg
    22081 gcctgttaaa tattaaaata ttcctggaaa cacatgtttc ttttatttta cctccggatc
    22141 cggctttgtc tgacactatc agctgaatat tatattctcc cggccttgtg aaagtttttt
    22201 tcacgttctg ccctattgcg gctgtttcat catcaaaatt ccagtaatat tttgcaatct
    22261 gccaacccgg aagatttgtt ttatctgcgc tgagttgaat ctcctgtcct gtatatgcgg
    22321 tatcgggtga ggttataaag ggttgctcaa tatcctgaat gaccagccgc tcagattttt
    22381 cattaaggga tttctccccc gtcaccaggt taacgacatc cagctggacc aggtagcttc
    22441 caggtccggc atagcaatgc tgcacaattt cacccgaagc tgtgtcgccg tcgccaaacc
    22501 gccattcgta cctgaaagga atcgtatcat attttacggc attttcctca atgaactcat
    22561 agcagtaggt attttcctgc agcgtatcgc aggtggcttt tctgataatt gttatgatga
    22621 actcatagat gtcgtcgttc ctctgccggt tggaagcaaa ataaccgctt tccaggtcag
    22681 gcatggccac aagtgcaaaa tcgtccgaag ctgaattgac aggctcgggc agccttacag
    22741 gttcgcccca ctcccggtcg tttttttcag aatagtaaac atcaaggccg ccggcgcctc
    22801 cggggcgatc ggaggtaaaa tagagccttc cgccagaatg aaggtaagga taattctcaa
    22861 tgtatgtgga attcaccatc ggccctgcat ttaccggatc actccattca ttgttaatca
    22921 attcacagta ataaatgtcg gatcccccgt atcctcccgg catgtctgac gaaaagaaaa
    22981 gatactttcc atcgctgctt accgaaggct gacctatatt ataatccgga ttattatatt
    23041 tgaagggacg tatggatgta atctgaaatc cgttcagatc ggcaataaaa atcccgcttc
    23101 tgttcctgaa ctttttgttc ttcgactgcc cgccggtttc tatctcactt gtaaaatata
    23161 ccgtttttcc gtcaggtgct atggaaagag gtccgttatt gaaaagttca cttctctcac
    23221 tcctgatttc ttttggtttg ttccactcaa ccgagtccct gcgttgaaca aaataaatat
    23281 tgaacaatct ccttccgtcg aaggaggttc gctccatgat cccgctcagc cgcctgtcgg
    23341 aacagaacag cagtccatca ttatacatta ccggggagat atcattatat ccggaagaac
    23401 tgaaaggtaa ccttgtgatc tcatatgaat tgagctcctg ggctgttgaa tttacaatta
    23461 acagacccgg aaacagaaca agcaatatga ggcgcaatct tttaatcatg tcagaaatac
    23521 ctggggtttg ccgcactaac cttgtatctg aattcatacc tgagaataaa ctcagttgaa
    23581 ccttttgtat atgtattcat cctgccgtat ggataatcat atgagaaacc tgccatcagc
    23641 tgattattga tctgaaactg taaaatgccg acggccacct gttcagagat acgccaggaa
    23701 gctccggccc agataatatc ggcaataatg aagtttgcat ttatatcaaa ctgtgaaacc
    23761 ttttttgaat tgcttaatga ataattcagc aaaaaagaag gtttcagctt cagcaggtcg
    23821 ctgacaggaa tgagtgaacc tgctgtaagc ctgaattcat actgtccaaa gctgtggtat
    23881 ggcaccgttg agccgttgct gctgcgacgg taattcagga aggaggggat ggagagtcct
    23941 gcaaaatatc tgtcttcagc ataatatgca cctgctccca catttggcat cacatgagtt
    24001 ctgtcatttg acttaaatac aggatctccc ggatcgttca gaagcaggct ggcataatcg
    24061 gtttttgata tatctgctcc ggcattcagc ccgaatgata attttccctt tctaagttta
    24121 atatggtaag cataatcagc ataaatactt gttgcttttg ttgccccgta ctgcataaac
    24181 tgcgccgtca tccccagggc aaccttgtca ttcttcattg gtgagtggag cgaaactgac
    24241 tgaatcatag gggagccctt tgttccgatc cattgaatcc tgtatgaaag aagactgctc
    24301 agcactcccc gtgttccggc ataggcggga ttcagtatta atccgttctg cagatactgg
    24361 ctgtgaacgg gaaatcccag gttgatcctt gatgaatcat ggacactctg ccctgcagca
    24421 atgcagaata caaacaaaaa aacaacaata tttaatcctg tccttaccat aacggtaaaa
    24481 catttttcag cagaaccata taacggagtc tttccataat tcaatacctc ttgagaatca
    24541 caaagccgct ctttttgaaa agctgactgg ttctcgtaga tgttattctg agaagataat
    24601 aatatgtgcc ttcgggaaga tccctgcctc tggagttttt cccatcccat gcatgccatt
    24661 cacttccgtt ccggttcgat gccgagaaaa ccccgctgcc tgcactgttg acaatggtca
    24721 gctcaacaat ctggttgtca agatcaagtc cctttatttc aaaagtattg ttgtatcctt
    24781 cagggtcatt gttcggtgag aatccttccg ggatgaatag ttcgttcaca ttgatttcaa
    24841 caatatcgct taccgtgcag gcgcctcttg ttactgtcca gagaaacctg tttaatcccg
    24901 gggaaagtcc tgaaacctct gttttttcaa atgtgtcatt ctgaaagctt cccgagcctt
    24961 ggagcacggt ccacttgcct gatcctccct ctactgccgg tccggccgac aatgaaaaga
    25021 tgttgtcaag tgaaaatata acagtatctt ttcctgcatt ggcaatgtaa tcatcaggca
    25081 gtttgtccat caccagctca actatatttg aaacactctg gcagcatttg tttttacccg
    25141 acgccacatt tctcctgtat cggatatcgc ctgtcatgac cgggggctgg tacgatccgt
    25201 catgattgtt attggtcccg gctgccggag tccagtttat cccgtccgtg ctctcctccc
    25261 acaggaaggt atacaccccg gagcctccgg aaagggtcat tccctgggtt tgtcccagta
    25321 ttccgggaac atcggccatg caaaccacct ggtcttctga aatactattg ttacttatta
    25381 ccgggagcac tgaaatcagc aggggtccgc tttccgatga acacattccc gaagtcgccc
    25441 tgcgtctgaa atatgtggtt gttgtcaggg tctgaggctg atagtcgcgg gatgttcccg
    25501 atgcctggat atcggtccag ttcctgttgt caggagaaga tgaccactgg taggtaaaat
    25561 cgcctgcctg ttcagtcccg cccgacggct gcgacccgat cagcctgttg ggtaaacttc
    25621 cggcacatat gattgtatct gagggtcctt cttccatgaa aaacagatta ttctccctca
    25681 ccggactatg gacatttatg gaaactgcct cactgtaact ggtacatact gaagaatgca
    25741 ctatccgcct gaaaaatgtt gaatctgtga gagccggtgg agcatagtcc ggcgaagtca
    25801 cattggtaaa ttcgtctata tttctccagg tacggccgcg ggtactgtcc tgccaggtat
    25861 agatgtaggt tccgggacct ttgccattca atggcaatga tccggcaagc agtgccggaa
    25921 ttgtccctgc acagatcgtc tgaccgaaag gcaggatggt attatttgta accggaggat
    25981 atgaactgag caatacggga gcgctttcgt tcacacatac attattggcg cccgacagaa
    26041 ccactctcct gaagtattgc tgccccggga aatagggagc ggattcggac gggttataat
    26101 ccgggtcgtt atttgatccg tcagcttcaa cccatgtggc tccgtcggca ctcctttccc
    26161 acctgaaaat ataattattg tcgccgcctc ccagtatggg tgcaacactg ccctgaaggt
    26221 ttgtgaaatt ctctccaaaa cagatttctt gcggatcgga tgatattgta ttattactta
    26281 tcccgggcag gacgcttatt cttacaggat cgctggtact cacgcatgcg ccggaaaaaa
    26341 ccctgcgcct gtaccaggtt gtctgcgtca gtcctgacgg agcctgatag ctttcgtcat
    26401 cagcaccggt tccggtatag gtaatgttat cggtacttga ttcccaaata aattcgtact
    26461 ttccattccc gtcctgcaag ggctgaaccg agtgtaaaat atcggggatc tgcccgtaac
    26521 acaagatccc gggatcgcct ataatgttgt tcctgattgc aggatgaact tctaccataa
    26581 ccggcatgct gatatcagta attgttgcac cgtagtccct tacgacccgt ctgaaccagg
    26641 ttgtggcggt aagtcttgag ggcggagtat aatttttctg ggaagagttg cccggggcag
    26701 tagtgaaccc tcctgacgga gatgtaacac tgctttgcca ttcgaaggta tatggcggtg
    26761 tgccgccaac gggctgggat ccgataataa gtccgtaatt ggtgtcggag catacggaat
    26821 cgatcatgtt attggttgca gccgtacgga tggaattaaa aaggaacccg ttaagcgcag
    26881 ggaaatagtt ggctctgatt tcacccggat ctccaccggc caaaggtgct cctgatgttc
    26941 cgccggcgac attagcgctg ctgacagctg caggcagggt tgccggactc agccatatta
    27001 gtccgccccc tccgccgcca ccctggccaa acccgccggg attggtacct ccgtttcctc
    27061 ctgctgctgc aagtccgaga aagctgaaga gattttgaaa tgaaagggcg atggaaccgc
    27121 cggcgccgcc tccgccggct cctgcatctg aaacagcatt ctgggctgtg gctccggatg
    27181 ccctgataaa atgatttctt gcgtcgatag tatcagcgat aattatgact attccacccc
    27241 cgtttccgcc agccgatgca gtggaaccgg cagactgggt ggaagcaccg cctccgccac
    27301 cggcaaaaat accgtcttcc acagccgtgc cggttatacc cattcctcca aatcctccgt
    27361 cgcggggatc atcaccgcac tgacccggta tatatttttc aaggccaccg tcaccgcctt
    27421 ttccacgatt tgaaccgcct ccgccacctg aatagcggcc attacccccg ccgcccccgg
    27481 aaaagttcct tccctgtccc tttgcatgat tgggataaag cagcgctcct gtgtaatcat
    27541 gcaccgaaat accctctccc tttagaccag cattgttcca tgacaaagga taagaatcat
    27601 gattattggc tgattcattc gtaaacacac attccccgat cccagcagaa ccctgtgccc
    27661 ctgaaaagcc tcggccggtt acatctatat cagcattcag ggtaagcttt cctcctgcca
    27721 gaaaagcaac tacccccccg gtaccggttg aaggatccca gggctgacag gttacagtcg
    27781 aggcaacttc aggcgatttg tagaaaggta cccggataag ctgaagattg ctgttggtgc
    27841 tgtagccgtt gatgctgaca taagtattcc ttgtaaaatc tattctgccc gtcacaatat
    27901 ttaccgactg tacaacaagg aattcatatc ccccgggagt tccgaaaacc gactgcacat
    27961 tctcaccata cgacccctgc accgtctgaa tgcccacacc ctgcatctgg ataagcagta
    28021 cataatcgtt gggtgaaaat tgtgcggctt gtgcaggatc agtgacaatc acaaaccccg
    28081 gaccaatgga agttaccttt gcataactgt tgacaatgcc tcctataact tcctgcccat
    28141 tgagaacttg aaaaacagaa ccagataagg ttgacaaaac agatatttgc aggataataa
    28201 tattccggat gaaataatgt ttcatcagta tgctgattga caagctaaac taaatcaaat
    28261 ttgttatatc cataaaatct aaaatccggt tactcctggt ttaccggcgt ctgatcgtct
    28321 ataaataacg ggaattccat ataaaagatt gtgccctttt ccggttctga tttaaaccag
    28381 attctgcccc cggcgctttc aatatatctt tttacaatcg aaagtcccaa accggctcct
    28441 gaagatttag ttgtgaaatt aggagtgaac atttttccct gcaccgattc ggggacacct
    28501 gttccgttat ctgcaacgga tactataact ttctggtgct caacttccat attcagcctt
    28561 attactcctt ccctctctga gggtattgcc tgtatggcat tttttataag atttgagaat
    28621 atgctgttca catgttccct gtcggcaaag atcaggattt tccttccatg ggtccagttt
    28681 accctgaatg aaatgttccc ggtattctta aaaagttcca gggtcgttct tattgattcc
    28741 accaggtcta ctttggaagg tttgggttcc ggaagccttg cgaacgatga aaaggcacct
    28801 gcaatggaag aaagattgtc tataaaatca atctggttgc gggtgaacct ctcaagcttt
    28861 ttttcaaagc ctggaacccc gtctttccat gatttaagca gctgctgaac attgagcttc
    28921 atgggagtaa gcggattctt tatctcatgg gcaatctgcc tggccatctc cctccatgca
    28981 tattcgcgtt ccgaaacagc aagctttctc gcgctttctt ccagctcatc aaccatcctg
    29041 ttgtattgcc gtacaaggtc gcccacttca tcatttccct tataaagcag gtgctcactt
    29101 ttctttccca gttcaactga ggcgagcctg ctgctcagaa ccgtcagtgg tgcggtgaga
    29161 cggccgctga taaacacggc aaggctcatc gctgccacaa tcagcagcaa agtaaaattc
    29221 attaccgtca ctatcatgtc ggagatatcc cgggcaagga cactctggag gcggaaatag
    29281 ggaaggttca ggtaaaatac tatctcattg tcgtgattgt agaaaggcat gtaagctgaa
    29341 atataataca gattcccgat cctttcgttc tggaaatatt ccgacttggt caggttggcc
    29401 agattcatga aggcaatatt gttcatccgc ctgcttataa gattcctgaa aaatatctcg
    29461 ggtcgtgagg tagccagcag attcccggtg agatcgtaaa gattgatgtc tgtattgaat
    29521 atattcgata acctgacaag tatctcatca agggaatagt aactattgtc tgaaattcgt
    29581 tccgcctgca gtctttcggt cattaaactg ttttccagtt caatgtaaac cgaattgagt
    29641 ttttccttca cattttcata atgcttatcc tggtaacggc ttattgccag tgatgctata
    29701 atatatccca ccagggtaaa tgagacaagg agaatggcga tgaatgacat ctgaagtttc
    29761 tgacggaagt tcatcccggc aggtctcatg aaaaccggtt ttcctattat gacgaaaagc
    29821 agattggaaa aaacaaagat gaaggcgaac aaataggcaa acgaaattat taaatcttta
    29881 aacgacaggg ccggcctgct gataatcacg gtaacgttcc cgttcctgta cagaaggtgc
    29941 cggaatgaat cctcgacaaa ggccctgtat cctgaatcgg aaataagata atcagtatcg
    30001 tttttattat atggataatc accggttctg agaaccagtt cgccgtttat atatttcgca
    30061 aatgaataat ccctgaggcg ggcatagctg tgatactttt tatcaagaag cagttctgag
    30121 tatcctggct ggaacacatt gatgtcactg taaatatcaa taaaaagacc atttatcctg
    30181 cctttgccat aattaaaaaa cagccggccc agataatttg cccttccctg cctgctgtcg
    30241 atataataaa attctgaacc agtcagcctg ttgccgcgcc tgagtatcct gtcattaaaa
    30301 aacctgaagc aatcatcgta aatttcgcct gtttctccaa tcctcagtga atcattgccg
    30361 gtgcatacaa taaaacttat attgaagtta ccccaatacc cctggaaata cttgccgcgc
    30421 atatattctg cgatcatttc aacgtcgtcc ttttcaaagt actcgacatt cattatccct
    30481 gcgagcaccg aatctgcttc gatgaccggc cacatatcga gcaggagatg ttcggcagtg
    30541 ggatcattct ctgttgagta ggaaagcagc tggatattga tcttttcttc tgtttttctt
    30601 tccgagttgc ttgtaacaat atagagtgaa tataatgcga atagtactga aaatatcact
    30661 gatttgttga atgttccggc atgaaataaa ctggttagcc tgatacctgc gattaccatg
    30721 atataaaaag cagcaattga aatagcttca aaaggcctat ttccggcaat tatcaggaaa
    30781 acggacaggg acagcacaca aggtattatc cgggatccgg caggccagtc ctttgatgca
    30841 agtaaaacct tgaggaggta gagaaaggga actgaaaaca gaaatgaaac tgtgataaag
    30901 cccatcagac tcaggctatt aagttcgagg actttataag tttcgaaatt tatattcgaa
    30961 ttcaggatca gatgactgaa gatcagatga tagccaagga acaggaatgc agcaacaacc
    31021 agcagtaagg taagccgcag ccagctttgc cttggtttat tcacaaaatc atcaacaaaa
    31081 tgcggatgcc tgaaaaaaag aaaagccgtt gcagccatca gaacagaaag gaccagcaga
    31141 tgaccaagcg agggaataaa tccgttcatg gtgtaactgt agggggagaa caatccgaac
    31201 tgggtcagaa tggccggata gctggtgagg agaataatta catagactga caaaaaaata
    31261 agcggacccg aaaagagggc aagagttccg cgcttgtgcg atacaagata ttccgttagc
    31321 cgtattacca ggaaaatgaa cagtaagaat gagattgtcc acatgagcat gggaacaatc
    31381 ttaaaatacc aattgccatc cttttcgctc ggatatacaa gagagaagag aaaagtgcca
    31441 tcggcagaca ccacatggta ttcagaagct gttttgtcag tactgaagcc cacttcgccg
    31501 gtaattccaa aatccctgag aaatccgctt tttattatgt cattctcaaa accataatcg
    31561 ttacgcagcc tgagaagtcc tacaatcctt tcattgccgg cctgaatgct ccgcgccagg
    31621 aaccatccgt tctgaacaaa aatcagcggc cgcaatagtg ccgaatcaac cagaaacccc
    31681 ggaacatcaa aatcattgtc tgaccagtag ataagtttgt tccctatata ttcaagaagt
    31741 gtgatcctgt tcttttcggc aagtgaaaaa agttttttct ccgaggagga accatggctt
    31801 tctcctgctg ccagaatggg ttccatgcca ttcaggcatt catccataac tttttccttt
    31861 tcccttagga ccttgttaaa tttccgcgtc ctgagccagt attcgaaatc gctgaaatag
    31921 accgaatcga cagcaattga caggattatc ataactgcgg tcaggattgc cagggaatat
    31981 ttaaaccggg gatgcttcat ttttgcaggt gacaatgttt aatcatgatg ataaggctct
    32041 ccttttatta cggtgaatgc cctgtaaagc tgttcggcaa acaacaatct cactacctgg
    32101 tgtgaaaagg tcattttgga tagcgacagc ctcagatcgg cccggtccaa tacatcaccg
    32161 gccaatccgt atgccccgcc tataacgaaa acgatccttt tcttctgaag catgaatagt
    32221 ttttcaagct tgccggcaaa ttcatgggtc gtatattcct gtccgttctc gtcaagaacg
    32281 attatatagt cctccttctg caaatattgc agcattttcg caccttccct gtctttctgc
    32341 tccttaacag gcatatacct cgtgttcttc agttcaggaa gagtgactat ctcaaatggt
    32401 gtaaatttgc gaatccgagc cgaataattg ccaaccccct catctatata ttcttccgtg
    32461 gtttttcccg tttgcagtaa aactattttc ataaattggc gcgacttttg tgcaggtaat
    32521 acagtacggc ccagtcgcaa atttaacaat ttaaaatgtg cagatataat tataattatg
    32581 cagattgagt atcggggatt tctacagtag ataaaataat ataaatttgc aggagaaaca
    32641 acctgacggc tgacaagaaa tatattgaat tgatgaaact gatcagatta ataaatgtta
    32701 cggcagcctt tatcattacc ctgggaatat cggcgcagga acaggcaagg atccctgccg
    32761 gaatatccat agccatcaag tcgggaaatg caacggaact gtctaaatat atgaactcca
    32821 ccgttgagct tctgctgctc cagaaggaag atttctacaa gaggaatgtt gcagaaacaa
    32881 ttctcaggga ctttttcaat gaataccatg ccaaggattt tgtcatcagg catcagggtg
    32941 caaggaatga tgcccagtat gccataggca acctggatac tgaaaaggga gccttccggg
    33001 tttattttct tctgaaaaag gttggcggtg aattactgat acaccagata cgtattgaat
    33061 ctgacaatgg taaatgacaa cctgcgcaaa atcattctga gaagtcttga agaagacata
    33121 ggtgacggtg atcattcctc acgggcttgt attccctcaa gggcgaaagg aaaggccaga
    33181 ttgctgataa aagaaaaggg aatcctggca ggatgcaggg tcgccctaga aattttcagc
    33241 attgttgacg gcgatctctc ggccgaagtg ctaattaaag acggaagccc gatggcaccg
    33301 ggagatattg tcttttacat tgagggaaga aagcggtcca ttttaaagtc cgagaggctt
    33361 gtactgaata tcatgcagag aatgagcggc attgccacaa ggacacggga atatgccgac
    33421 aggctttccg gattgaaaac caaggtactc gatacccgta aaacaacccc cggattaaga
    33481 ttccttgaaa aggaggctgt caggataggg ggaggcctta atcaccgaat gggcctttac
    33541 gacatgatcc tgctcaagga taatcatatc gactatgccg gtgggattga aaaagctatt
    33601 tcaagaacga ttgattatct gaatagtacc gggaagaagc taaagatcga aattgaggcc
    33661 cggaatcttg aagatgtcag gagaataatc aaggcaggcg gggtggacag gataatgctc
    33721 gataatttca gcctgggcga aacaaaggca gccgttgaat tgattggagg aaaatttgaa
    33781 actgaatcat caggaggaat aactcttgaa aacttaaggg actacgctga atgcggggtt
    33841 gattttattt ctgtgggagc ccttacacac caggtacgaa gcctcgacat gagtctgaaa
    33901 gcagtctgat ttccggaaat gccggggtac tcagctttct gatatacagg tcttacccgg
    33961 atctcccctg accatgggat attgtttctg taaatccacc ggaaatctta tctgatcatt
    34021 aaatatgcct gtccggaaat gacaccttac cggcatttat catcctggag gcctgttaat
    34081 cgtggggtgt ctataaaata tggctgcatt aagaatgctg ataattctcc atatttatat
    34141 aaattttgtt aaaagcacat taaaaaataa ttacttttgg ctcacaaaca gttaaaatga
    34201 gaaaatggat attttttcct gtttttatta ttctcttcag ctgcaaggga aaagtgccgg
    34261 aaacatatct ttcccacgaa caggcagcgg cttattttgc gaggattgaa gagatttgca
    34321 acagggacaa cgggaaatta tggggaagca atcttttcgg gccaatgatg tttgtcgaca
    34381 ggacaagcag gcagatactg gcaaaccagc ccgacaagga cgggatcctt aaacttaagg
    34441 acggaattta cacaggcata tacccaaggg agaaactgat caacattacc gctactgaat
    34501 ttggaggcac tcttttcggc gttgcaccct tacctccggt agaagatgaa taccgaatca
    34561 taacaagggc catccattgt cttttccaca gattccagta cctgaacggg atttatcctg
    34621 ccattttcaa ctccacaaac atggatgaaa gagaggcgag gatctggctg aagcttgaat
    34681 ggaaagctct gcgcaaggct atcacttcgg aaggcgagga taaactgact gccataaggg
    34741 atgccctgat attcagggga accagccaaa tatcctttcc gggttatgta gatgagggaa
    34801 atcattttga gacatacgaa ggactgacgt catttacctt tacctttctt ccctcggcat
    34861 caatggatga attcaaaaaa aggctttttg aatatctcga caggatctat tcctttcaat
    34921 cctattcaca gtcgtatggc ggtattcacg gtgcgcttta tgccaccctg ctttaccaga
    34981 aaggatttga tttcaaaaat ctcatcagcg ataaaaccga tttgggaaaa cttgtgatgg
    35041 agctgtataa cattgaactc cctgaaatat gcagggacgt tgcgggaagc ctggccataa
    35101 gctacaacct tgatcagatc tacagggagg aggagcaaag gctccgggac atcagggaga
    35161 ggctctgcaa acaagtcaat attttcaacc agaaacccgt ggttttcatt ggcctagaaa
    35221 gtccatattt cgatttcgaa ccggaagatg ttcattccct cgataccctg ggcacgctgt
    35281 acagccatat tagagtctcc gacaactggg ggaagctcac agtaaacaaa catggctgcc
    35341 tcatctcagg caattataaa cagctcagga taactgccaa agggcttaaa gccgataaaa
    35401 atcacattta cggcgaggga tggcacatga tactcaacga ggactgggta atcgatgaag
    35461 tcaatgataa ttattacgtg catcgaatag ccccctgagg aaataatccc ggagctgacc
    35521 gtgacctgca ccggatcaat tcagggcctc aagcgattcc tccagcgatc ggatcttcag
    35581 ttcggcatcg gcttttttct ttctctcaag ttccagcacc ctggcgggag cattctgaac
    35641 aaatctttca ttatcaagct tcttcattac cgaagaaaga aaacccctgt aatactcaag
    35701 atcagccttt aaacggttcc tttcattttc aatattaatg cttcctgcaa caggaatgta
    35761 aaattcagtt gtcccaacaa tgaaggaaac tgatccgttc tgcttgtcag atataaattc
    35821 aatctccgaa atattggcaa gctttttaat tacgggcaag aattcaccat caaaggcctc
    35881 cctgccggat cttacgagca gtcttaccgg atccctgtta agaatctgca gctccttcct
    35941 gacggttctg atggcactga caatatccct ggcataagcg aacctggtga tcagttcttt
    36001 gttatgtttc ctggatgaag gcatcctgct gatcataaga ctttcgccat ccttccgggg
    36061 cgaaagaagc tgccatattt cttcagtgat gaaaggcata aacggatgaa tcagatgaag
    36121 gagggagtcg aaaatctcaa tcgttgcatc ataggtgatt ttatcaactg gcttttcata
    36181 ttccggtttg attatttcaa gataccaccc ggagaactca tcccagaata atctgtaggt
    36241 actcataagg gcttcggata ttctgaattt tctgaagttg gcatcaattt ccccgaccga
    36301 cttccttaat atctcattca tccatctgac cgtgatcgcc gaagattcgg gctgaggtat
    36361 ccggtcatcc gttttccagc tttttacaag cctgaaggca ttccatatct tgttggcaaa
    36421 attcctgccc tgttccggga gcgaatcatc gtaaagcaga tcatttcctg caggggagca
    36481 aagcatcata ccaaccctca caccatcggc cccgaatttt tttatcaggt cgaggggctc
    36541 cggggaattg ccaagcgatt tggacatttt ccgcctctgc tggtcccgga ccagccctgt
    36601 aaggtaaaca ttcctgaaag gtttttctcc cctgaattca tatccggcaa taatcatcct
    36661 ggcaacccag aaaaacagga tctcaggtgc agtaataaga tcattggtgg gataataata
    36721 tctgagatca ggatttcccg gatcgttaat accgtcgaat gatgttatgg gccagagcca
    36781 ggaggagaac caggtgtcaa gtacatcctc atcctgtttc aggtcatctg gtgcaagtga
    36841 attgtttccg ctctgcttcc tggccaggtt gagggcttct tcagcgttct ccgccaccac
    36901 tgtttccccg tctccataat accatgcagg gatccggtgg ccccaccaca gctgcctgct
    36961 gatgcaccag tcatgaacat tttccatcca gtgcctgtac aggttcctga attttggagg
    37021 gtataaatga atatccccat ttaatacggc atcaagtgcc ggtctggaaa tattcttcat
    37081 tttcaggaac cattgtgttg aaagctttgg ctcaattact gaatcggtcc tttccgaacg
    37141 tccgatcttg ttgttgtagg aaacaacctt caccaggctt ccggtttttt tcagctcctg
    37201 ttcagcaagc ttccttgcgg taaacctgtc tgtcccctca aatttgcctg ctcgcccgct
    37261 gattgtccca tcgtcattaa aaatatctat tgatggcaaa cggtgccgta ttcctatctc
    37321 atagtcgttg atatcgtgtg ccggagttat tttaaggcaa ccggttccga actccatatc
    37381 cacatagtca tcaaaaataa acggtacttc cctgcctgtc attggtacaa tgaccttctt
    37441 tccctttaga tgcaggtaac gttcgtcgga aggatttgcg caaacggctg tatcgcccag
    37501 tatcgtttcc ggacgggttg ttgcgatcat tacataaccg tcttccccgg aaattttgta
    37561 tctgacataa taaagtttcg atttttcttc agtgtaataa acctcttcat ccgacactgc
    37621 agtcattgcc tcaggatccc agtttaccat tctgacgccc cggtaaataa gccccttgtt
    37681 gtaaagatcc acgaaaacct taatcacaga gtcgtatctt ttttcatcca ttgtaaagag
    37741 ggtgcggtcc cagtcgcatg aggctccaag ttttttcagc tgctccaata ttatcccacc
    37801 gtgcttttca gtccattccc atgcatgttt caggaattcc tcccttgtga ggttgcgctt
    37861 ttcaattccc tccgccctga gtttggcaac cacctttgct tccgtggcta tggatgcatg
    37921 atcagtgccc ggaatccagc atgcattctt tcctagcatc cttgccctgc gcacaagaat
    37981 atcctggatc gtattgttga gcatatgacc catatggagt actccggtca cattgggagg
    38041 tggaatgact atggtatagg gttcacggtc atcgggaata ctcctgaaaa atccatgcct
    38101 catccagtag ctgtaccatt tatcctcagc tttagcaggt tcatatttgg aaggtatgtc
    38161 aatcatttgc aaagagctga aatttttatt gaaagtgcaa aagtataaaa attcagatca
    38221 ggcggcaagc ttaagctgct taattgaatt atctttatct cctcctgact gcaggaaaaa
    38281 tggcacattc atcttatttt tcttaacttt gtattattag ctaagtaaca actaaatctt
    38341 tctgtttatg ccttcacttt cagaaaaagg tattttgata ccgccgtcac ctataagaaa
    38401 actggtacct ttttcggaag aggccaagag gaaagggaaa aaggtttatc atctaaacat
    38461 tggtcagccc gacatatcca ctccggcaat tgcactggat gcactccgga acctggacct
    38521 aagggtgatt gaatacggtc attcagccgg taatgagagt taccgtcaga aacttgctga
    38581 atactacaaa aaggctggta taaatatcga tcatactcag atgcttataa ccacgggtgc
    38641 ttcagaagcg attctgtttg ccttttttgc ctgtattaat cccggggaag aaattattac
    38701 ccctgaacca ttctatgcca attatagtgg ttttgccgtt actgccggga taacaattac
    38761 atctgttacc tcaaccattg aaaatgactt tgcccttcca ccggtttcgg aaattgaaag
    38821 gaagatttcc ccaaagacaa agggaattct cctgtgcaat cccaataacc cgacgggtta
    38881 cctgtattcg gaaaaggagc ttcaaatgat cagggatatt gtttccaggc atgatctgtt
    38941 cctgttttcc gatgaagcct accgcgaatt ctgttatgat ggaatgaagc atatatcggc
    39001 catgaacctt gacggtattg gagacaatgt agtgctgatc gattccatat caaaaagata
    39061 cagcatgtgc ggagtcagga tcggagcact gatatccagg aatagcgagg taatctcttc
    39121 ggcattgaaa ttcgcacaat cgaggctgtc acctcccgga ctgggacaga ttgctggtga
    39181 ggcttcaatc gacacacccg atgaatattt tctggaagtg aaccgtgaat ataccatgcg
    39241 acgtgatttc atggtcaatg cactgaacaa aatacccgga gtttattgcc ccaaaccgat
    39301 gggtgcgttc tatacggttg tgaaactgcc ggtggatgat tctgagaagt tcgcccaatg
    39361 gatccttgag gaatttgatt acaaaaacca gactgtgatg atagctcctg cttccggttt
    39421 ttactcaaca cccggtctgg gatggaacga agcccggata gcttatgtcc tgaacgtcga
    39481 agatctgaag aacgccgtgg aaacactcga acaggcactt aaaatatatc ccggaagaac
    39541 gatccagtaa atgaacaatg ttctcagatg catagcaatt gacagtgatc cgcttgtcct
    39601 ggatatgatt ggggagttct gcacaaaagt ctattatctc gatcttacgg gcaaatatac
    39661 ggatcctttc agggcatacc agatacttaa cgaaaataag gtcgacctta tgttccttga
    39721 cgtgaggatg ccacagatca caggggctga atttctgaat tcactctaca atccgcccat
    39781 ggtaattttc acatcttcat ttaaggaaca tgctctcgaa ggttttgaat gtgatgctgt
    39841 cgattatctt gtgaaaccct ttgccttcga ccgttttttc aaggcagtca acaaggcttt
    39901 ccagttacag aaactgaaaa agccggttga ttatctccag gatgagaatt ctcagccagc
    39961 tcagcgtttt ctgatggtaa aagttgaata cagcacgatc cgggtcgatc tcagggaaat
    40021 cctgtatatt gaaggtttga aggattatgt aaagatccac tgcggatcaa ggcttctgct
    40081 taccaaaacc acgatgaaaa acatactgga aaagcttccg caggaaatct tcttcagggt
    40141 gcataagtca tacatcgttg ctgttgaaaa gattgacatg attgagaaca gcagaataat
    40201 catcgggaat cagcgaatcc cgatcggtga atctttccgg agctcctttt tcagcctcgt
    40261 aagtaaaaat cttgtctgac cctttacaga tatcttctct ttataaacca caggtcgcgc
    40321 agcgacaggt ccagatgaac ggtcacaaaa ttctctctga agaggtcttt tttcttaacc
    40381 ccgttttgtc ccagttcgac agagacgttc actgtgctca gcactccttt caggggtatt
    40441 ccggtcccga aagaaagagc ataataatca attggtgtat tgttgattat gaaatacgat
    40501 tcccggtatt cggcgcccac cctgtataat aacatcctgc cggtcccctt gttcgggccg
    40561 aaggaaggaa attccacgcc agcggagatc ctgtcacagt tccgggaacg gatcccggag
    40621 ttcttaaatt ccgttccttc ccacaggctc cgctcaaaat cgatacctgc ctggaaaaat
    40681 ccccgttcat aggccagtcc tgctccgaaa ttcctgggga tgctgtattt actgctcctg
    40741 cccctgatca cttccgtttc ggtgtttgtc tttatggtcg tggtattttt ggcgttaagc
    40801 ttctttgaat caccaaagat caggcccaca ctgaaattat tctcatttat ggcaaatctg
    40861 taattaagcc cgtaattcag gtgaaaattg gaaagatagg taacatcctc aagggtataa
    40921 taataggttt ctgacagctc ccggtgagta atgttcccga aaagccagga tgcatttact
    40981 cccagggcaa gatttttggt aatcttgaaa gaggtgctca gaaaagtgcg gttaattccc
    41041 ccatcgcctg aaaatacctt ggaatatttc aggttgcttc cttccacatc actttttgta
    41101 ttgatattgt atcctatcgt actgtatggt gcaaatccaa aacttgtcgc cagccaggga
    41161 gttattctga atcccattac aacgtacctg aaattggcat cgattgacga ctggtttttt
    41221 gtaaaagagg cataagatgt atactttccg aaaagcccga tttcaaatat tgaaaccaga
    41281 ctgtcaagtc cgctgaatga agcagggtta ctgatattaa gatatgtggc agatctgaat
    41341 gcaatactgg tcccgcccat tcccttcccc ggacccaggc tgctcgtttc caaagagccc
    41401 agtccaaaaa gggaataggg tgatgatgaa ctctgtgaat ttgcattata catatgca
//
LOCUS       DAOOYG010000009        41140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_801_length_41140_cov_7.864914, whole genome shotgun sequence.
ACCESSION   DAOOYG010000009 DAOOYG010000000
VERSION     DAOOYG010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 41140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_801_length_41140_cov_7.864914"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1230
                     /locus_tag="PK288_01780"
     CDS             244..1230
                     /locus_tag="PK288_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HPM17673.1"
                     /translation="MKRILGIGNALVDILILIDDDGILEAFDLPKGSMQLVDIKKSEQ
                     IQDNTRNFSQTRSSGGSVANTIHGLAMLGIDAGYIGSTGKDATGDFFENDLKAAGVKT
                     YLTRRNSVTGTALTLISPDSERTFATHLGAAVELHPGDLDPAWFREHDILYVEGYLIT
                     DAALVEKACLLARESNMTIALDLASYNVVEAYRDSFKKIADRYVDILFANEMEAEAFT
                     GVPSMQALDLMGNSVELVVIKAGANGSWLRKGKKTIKIDATPVRCADTTGAGDLYASG
                     FLYGYATGMDLEKCGLIGSMMAEKVIGITGARMSKTEFTEIINNIGKIVQGP"
     gene            complement(1273..2547)
                     /gene="fucP"
                     /locus_tag="PK288_01785"
     CDS             complement(1273..2547)
                     /gene="fucP"
                     /locus_tag="PK288_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HPM17674.1"
                     /translation="MNDDLSVGVNRQKLVYEGFLVPFILISSLFFLWGFAHGCLDVLN
                     KHFQELLNMSKAKSALVQFVFYGGYFFMAIPAGLLMQRVGYKRGIIFGLLVFACGAFL
                     MLPATFIQTFGSFLFCLFVIACGLTCLETAANPYTTVLGPPEYGERRINLSQSFNGLG
                     WIAGPLVGGMLIFSANGNGNSNKFATIALPYMFIGTLVLIVAVFFWRVKFPKLKEDMQ
                     TGDPGQESGGRLKDLFKYPHFVLAVIAQFLYVAAQTGVNSFFINYVTEEIPTLSNQQA
                     AQILGFGGMGLFWLGRFTGSTIFMRLIRPNRLLAFYASANVITMILVVAGLGWTSVVA
                     LFTTYFFMSVMFPTIFALGIKELGPLTKKGSSLLVMSIVGGALIPILMGRIADVTNMA
                     LGFTVPLVCFAFIVYYGISGYRVKTPGLAASS"
     gene            complement(2704..3276)
                     /locus_tag="PK288_01790"
     CDS             complement(2704..3276)
                     /locus_tag="PK288_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPM17675.1"
                     /translation="MKKTITLVIFLSLLAVNLHSMEKWIADKTRTKLEWLGEKVAGQH
                     SGTINLHSGWIAWDGDKITGGEFLIDMASIQDADKSQKLEGHLKSDDFFGVEKYPVSK
                     LVITGSTSFAKGTGVVNGNLTIKDITHPVEFKASLQKSDDGLWYFANIVVDRSKYNVR
                     YGSGSFFDNLGDKIIYDEFKLKVNLLVKKA"
     gene            complement(3362..4141)
                     /locus_tag="PK288_01795"
     CDS             complement(3362..4141)
                     /locus_tag="PK288_01795"
                     /inference="COORDINATES: protein motif:HMM:NF009386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HPM17676.1"
                     /translation="MNGKPVAVITGASRGIGRSVAVALAGEGYDIAAIARSVDSEGMK
                     TLEPEIESRGAQFFPVGLDISCIKYHKEVVSNILERYGRIDILVNNAGVAPLQRHDVL
                     EMSSESYDRVMNINLKGPVFFAQKVAREMIWIKEQIEHYRPIIIFITSVSAVLTSVNR
                     AEYCISKAGLSMASAIFSDRLTSENILVFEIRPGIIQTDMTAATRDKYDRLIGDGLVP
                     QNRWGLPDDVAKGVASIARGDWDFSTGMTFEISGGLNIHRL"
     gene            complement(4205..6196)
                     /locus_tag="PK288_01800"
     CDS             complement(4205..6196)
                     /locus_tag="PK288_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPM17677.1"
                     /translation="MENEVIRLNGFSVNYRSTKTLIIGSGAAALNAAVSLHSMGEPDI
                     IIATAGWGWGTSNNAGSDKQTYYKLSLAGDVPDSVTDLARDLFRGKCMHGDIALCEAQ
                     GSVRAFMNLVNMGVPFPHDKYGSYAGYKTDHDPRARATSAGPYTSKMMFRVLSEEVLR
                     RKITILDRHYVISLLTDREKTRVVGALAVNILENDPVNAFTVFNASNIILGTGGPGDM
                     YETSVYPESQTGSVGMAFKAGASGQNLTESQFGIASLKFRWNLSGSYQQVLPRYYSTA
                     PDGSDPREFLNDFFPDYRSITRAIFLKGYQWPFDVRKISGYGSSLIDLLVFREREKGR
                     RVFIDFMRNLTWDGCQAFKPEELDPEVYNYLAYSDSLKPLPLERLEALNSPAISLYMD
                     HGIDIRAEALEIGICAQHCNGGLKGNIWWESDLRHLFPVGEVNGSHGVYRPGGSSLNS
                     GQVGGHRAAFFITKRYQTPPPEKSVFLSETVPAIEKDFGRAADWLSGVYHGSTPEQAM
                     KVIRKRMTDNGSILRSGAKIMKAVEEARIMYDDFDKLCAATSVKELAECFMGSNSCLA
                     HLVYLEAIRTYIAGGGRSRGSFIVDETKESAGTDPGNDSQFPILCSYDRDIEEKIIEI
                     RYDGEKINTNLAPVRMIPEQNLWFEKVWKEFREDIFLQD"
     gene            complement(6186..7496)
                     /locus_tag="PK288_01805"
     CDS             complement(6186..7496)
                     /locus_tag="PK288_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17678.1"
                     /translation="MHERFHYRTKDELIRKASELGFTLPFSDDVSPLLESAKAGEIII
                     PNRIVVQPMEGYDSDNEGAPSELTERRYLRYSAGGSGTIWFEAVAVNHEGRSNPRQLW
                     INRKSRDKFAFLNERIRKNSGLENRDPFLVIQITHSGRYSKPDGKPAPRIAAPNPLLD
                     RGTPEILTDDDLRRIQDQYIIAARLARDAGFNALDIKACHGYLMIELLAAKNRRNSIY
                     GGEEPEKRFRFFLETFDRIRYEVPGLMVTSRLGISDMYRGGFGVDDMDQPDFTEAMML
                     VGQLMDRGIELLNITMGSPYFNPHISRPYDVPLPGMKVPDEHPLQGVMRMINATSVFQ
                     KKFPDLVIAGTAFSYLRQYAPNVGAALVKSRDVSLAGFGRNSFAYPSMPLDLMRTGRA
                     DPSKTCICCSGCTRLIRNLHIGGCVIRDKEIYGKELKKLIADGK"
     gene            complement(7574..8887)
                     /locus_tag="PK288_01810"
     CDS             complement(7574..8887)
                     /locus_tag="PK288_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17679.1"
                     /translation="MTINKIGKLSVFIILISASLVLEAQDNELQTGPVVVSSDTAATR
                     SNHSREGAVKVFLDCRNCDMNYIRQHVPYVNYVRDVKEAQVYVNVTDQPTGSGGTLYT
                     YTFQGQKNFSGMNDTITFATVPDQTGPVIREKRTTILKIGLMRYIAKTPLIDEITIGH
                     EPGLELEDVKDRWDNWVFELSSSPRYSAEESAKRFQIWNALEISRITPDLKIEANLNQ
                     NINKQRFIDSETDTTYSKSNNSLHLLVVKSLGNHLSTGIRWENRTSTEANINFTTEIM
                     PAIEYDLYPYSEATHKQLRLLYAIGYQYSNYIDSTIYEKLYDNFFKHSLMVAYQIQEK
                     WGSINISLSETNYLSDLSKNRVNLWGYIRLRVLKGLSLSVNGSVSYNNFQPNLRKGEI
                     SEADRLLQLKQLASSYFVQGGVSLSYTFGSIYNNVVNPRFGSGYF"
     gene            complement(9015..10004)
                     /locus_tag="PK288_01815"
     CDS             complement(9015..10004)
                     /locus_tag="PK288_01815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM17680.1"
                     /translation="MIDISVIVVCFRGWARLRKCLDSLAGFRGDNFSYEVIVVDNNSG
                     DQVIHDFVRKYPDFRFILNPVNGGYANGNNLGCRNASGEYVLILNPDTEADENEIARM
                     LEEARAHPEYSIISCRQVNDRGKETTATGDFPEFSNLTGFQRSVARIFRKTAAPVTDN
                     GHVTFPDWISGSVVWMKRDLYLQLGGFYEGFWMYYEDVDICRRINREMGKVVRLNNVT
                     IIHNHGGSSRVNKRIAAITKTEVQISRHLYVSRNIKGAEGVLTQIFLVVNNIISGVIL
                     AAAGLIFFFVPALFLRTRIFLRLLGYYAGAALRQSWIGPRSVLYPGRMLKKAN"
     gene            complement(10063..10761)
                     /locus_tag="PK288_01820"
     CDS             complement(10063..10761)
                     /locus_tag="PK288_01820"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPM17681.1"
                     /translation="MQFPGLNITEGEALLHRKKMLLSMIIPLVIISLMWILKTAEVLF
                     DLDFTHLGILPLSVRGLPGIVLSPFIHSDFEHLFNNTLPLFFLATALFYFYSEIAVKI
                     SLWTWFLTGSFVWLAGRTSWHIGASGLVYGLASFLFFSGIIRKYFRLVALSLLIVFLY
                     GSMVWGIFPDVVRKNISWESHMLGFISGIVLAVAFRNEGPQDPVPVWMDDEEDGSDRI
                     SDTYGPETEDGKKV"
     gene            complement(10801..11784)
                     /locus_tag="PK288_01825"
     CDS             complement(10801..11784)
                     /locus_tag="PK288_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM17682.1"
                     /translation="MEKRYKWGILAPGKMAGKFTNALKHLDNAELYAVGSRDLRRAEK
                     FAADNGFRKFYGSYRELAEDPGVDIVYIASPHSHHREQAILCMKNGKAVLCEKAFAIN
                     EREVEEMISEASRNKVFLMEALWPPFQPFYRKASEILESGIVGRLLHMECFFSFNPPF
                     HPDDRKFNPELGGGSLLDIGIYPVIDALTFAGVPDGIKAYASFGKTGAEESVSAIFRY
                     NDGRMASIYSSFRTNAGIGCVILCERGRISLSRGRDMNQTVILEPDDMEKETYVFNPP
                     AMGYHWEAGEVMRCLDDGLTESPVVPLSFSRNLIRTLDRIRHDAGIRLPQD"
     gene            12322..12912
                     /locus_tag="PK288_01830"
     CDS             12322..12912
                     /locus_tag="PK288_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPM17683.1"
                     /translation="MKITVSINLGGYSFNIDEDAYTELKRYLKSLEFNLAGEESSAEI
                     ISDIEARMAELFRARLTGYKQVITITDVRDVIAVMGNPEDISDKEGMSSGAKFTEPGA
                     HRMYRDPDRRIIGGVCAGIGAFWQIEPWIVRVIFIAVAMAGGLGILVYLILYIVLPEA
                     RTTAQKIEMKGDPVNIHNIKEAVKREFDNVRKNMNL"
     gene            12950..13276
                     /locus_tag="PK288_01835"
     CDS             12950..13276
                     /locus_tag="PK288_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPM17684.1"
                     /translation="MDIENTKAQMRKGILEYCILSVLSKASCYASDIIRELREAELIV
                     VEGTLYPLLTRQKNAGLLSYRWEESQQGPPRKYYELTSEGREYLAGLDKSWDELVESV
                     RLIRNR"
     gene            13306..14910
                     /locus_tag="PK288_01840"
     CDS             13306..14910
                     /locus_tag="PK288_01840"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPM17685.1"
                     /translation="MNRTIKINLGGILFQIEGEAYNMLRGYLNEIDARIGKLPDGAET
                     LEDIEARIAEIFQSQGAAAGVISADNVGEMIKIIGRPEEFETGDGQEGRAREHTRVPR
                     SRTLYRNPGDSIIGGVCGGLGSYLNIDPVWIRVIFIVFAFFAGVGFFVYLALWIALPI
                     ADPETIKRESYMKGADHADFGLEKRSVAGTTASRHPENACPERLAGALNEILRAGGKM
                     LFIALRIFMVLLGLAILLSGFLALLGLIMVFIFRFPGIYSPHAHGLNFSWLFDFLKYA
                     VNPATFPWILTLTFIVMLIPLLAIIYLGIKMIFWFRARNGMISLAGFLIWLVCLAALS
                     VILFNEGIGYAETAKKDAGEVLKKTTGNIYILSGNKINDIRYEREISFPFRFYRMYFT
                     DDLSNPCITPSLVIGRSNDDYIKINVRKRSSGKTRSEAAGRMEQLAYNYSVEGDTIFL
                     DEYFRIPEERKWSFDNIVVSLYIPEGTRICFSRGTENMLRTDWSCNYGCSKPETLREY
                     GAKESGKPESEWIMTEDGLRSRSLGQ"
     gene            15169..17526
                     /locus_tag="PK288_01845"
     CDS             15169..17526
                     /locus_tag="PK288_01845"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPM17686.1"
                     /translation="MKTHKGYLLLPVVAALAIVSCQPGRKAAVPEDLTRYVDPLIGTA
                     HCRWFHVAPGASPFGMAKPGPSTDGHLGNLSGWEAVGYDFRHKSIEGFPNFHEFQVGG
                     VVFMPTTGELRTVPGDVDSASGGYRSFFDKKDEILEPGYYSVILKDYGIKAELTSTPR
                     VSFHRYTFPAGDRSNIIFDIGNRQGESGEVTDARVWLSDDGRVEGFVETYPEYVKKYQ
                     TGASVKMFFSAIADKSPVSWGVFNGPEVRHGEKETSGRGAGLYLTCKTTGNESITIKA
                     GLSYTSVENARLNLETEAKDMGFDEAREKARDAWNSYLGRIRVEGGSAADRIKFYTGL
                     YHALLGRGLASDVNGAYPKHDGSIGQIPAGPDNKPQFNFYNTDAIWGGQWNLIQLWAL
                     AYPEYLTDFVKTHLTVYQDAGWLGDGLACSRYVSGVGTNQVPLAIAAAYQCGIRDFDI
                     QKGYEASLKNEIGSENRPFGAGKADVGKFVKYGYVPYLAKGEGPDERWKFSCSHTLEY
                     SFTSFAVAQMAKSLGREEDYRLLTDLSHGWERIFHPQRKLMWPKLENGKFVENFDAAV
                     PHDGFQEGNAFQYSFYVPHDPEGIVAKIGREEFNSRLDSTFVISRKDIFGGGTEIHAF
                     AGITKPYNHGNQPCLHTSWLFNHSGRPSKTQMWVRAILNDFYGTEGIHGYGYGQDEDQ
                     GQLGAWYVMSAMGLFDVKGLTDTDPSFGIGSPLFDRVTVTLNNRYFKGREFVIETENN
                     SADNPYIQEVSVNGKILSSVFVPFTDITGGGKMVIKLGNTPKDLP"
     gene            17528..17680
                     /locus_tag="PK288_01850"
     CDS             17528..17680
                     /locus_tag="PK288_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17687.1"
                     /translation="MKSGQNYPEPAWARLVSLFCHPFLTIPLFVAIMMFAFNDVWEAA
                     NSIWLH"
     gene            complement(17721..20099)
                     /locus_tag="PK288_01855"
     CDS             complement(17721..20099)
                     /locus_tag="PK288_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPM17688.1"
                     /translation="MKLLSSFSGLIPAGVFFAMMSSALLLEGQELKKPIVFPIPREMQ
                     LTGEEFPLSEEAVIIVPVNASENDMFLARSLSGELSSKYSIAVKTERAATLPENKKVI
                     LMGTAGNPLVKKYCQSGKLELTSRNPGPEGYLLHVDAGLVVVAGWDDAGAFYGLQSLR
                     QLIRNGNGNTVTGIRVRDWPQMPFRGIRLYVPGPDNIPFFNRFIRDFMALYKFNKLIM
                     EVNCMRLDRHPEVNAGWVEFSKYMQYTRSNSTEGPHGEVKNSSHHDAGDGHILEKADI
                     RNIVELSNRNFIEVIPEIPSLTHAYYLLTRHPELAEYPGDIWPDTYCPSNPASYELMF
                     DVYDEYVEVMKPKMVHIGHDEWWGAPLGVCPVCRDKDYSVLFAEDVKKIHGYFTGKGI
                     RTAMWGDYLLESVRDTLFMDWTSSTGIKYRTPGAVRPEVVKELIPKDILIFNWFWDSE
                     KKEKDLNDFGFTQVYGNFTPAITNWEERSGKNNLAGGAPSSWASTNEFNFGKDLLLDF
                     MGCANLLWSPHGISLPELGSVVREAMSTVREDLHGARIPGRDGDPLMPVDISQYANMT
                     AGTEAFNMDLRGLLTDEIKKGPLYFRMVNPAGTYGNMLVAVGSAGTGPVHLPDQVNGI
                     VIDEDVSSLIFLHACALPAANQKAYFNIPNFFDSADLLGWYEIIYEDGFKEIIPVQYG
                     VNILEWNPGGEKSIDRLQGNTGSPQSAYCYMADPVPCSEEGRYPPVTFFAFEWTNTRF
                     GKVIKEINLCGTTNYQTLQQDYVKVVTEPMKSNAIMLAGITKVQKRKPFIPQ"
     gene            20426..22144
                     /locus_tag="PK288_01860"
     CDS             20426..22144
                     /locus_tag="PK288_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17689.1"
                     /translation="MNKSKNILLTLFLAAVCIAVILLARTNSSGRNITILNPFNDALF
                     PAEFPAPAFEWRSQLQDPSPWEVTLSVRKNDYSITDITDKTIWRPEEPAWDSIKKLSE
                     NGKIFFRVRKHGNGGRGKQVVFRISPDSVGATVLYRQMPIPFVLAERELESMNYMLID
                     PGSPSPPHIAMKGFPVCGNCHSFSDDGKLLGLDLDAGRRDKGGYFLTAISDTVVFDSD
                     NFMSWSKHEKRSTFGLFSKISPDGRYVVTTLKDRVVAENPPLNSAENFAYSQLFFPVN
                     GHLAIYDRELKTLNDLPGANLEEFVQSNAFWTPDGKSIVFSRAKALPRSDNVYEITVS
                     DREVLDSFVSGKKPFRYDICIIPFNNGKGGTAVPVKGASGNGKSNYFPTVSPDGKWII
                     YCQANSFMNLMPDSRLYIVPLKGGTPRLLDCNMYAMNSWHSWSPNGKWIIFSSKGLNI
                     YTDLFLSHIDKKGNASIPVLVDRAREYQRVCNYPEFINRNPKNKFSMKYDYVELAHIR
                     YALRDKDPEKARQLFYRLIKQDPFMFSEDYSELSGFLYRMGLKEEAVKYAEIAAKSLK
                     SNIEAD"
     gene            22364..23284
                     /locus_tag="PK288_01865"
     CDS             22364..23284
                     /locus_tag="PK288_01865"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPM17690.1"
                     /translation="MRLIRIISFPAVAAAYFVLTSCSDSGSDKYYKSSVIVPVNTFTI
                     GVEGPAVDRHGNVYAVNFETEGTIGKITPDNQAGLFIRLPEGSIGNGIRFDSRGNMLI
                     ADYTMHNILKVDMATGQISVFANEPLMSQPNDIAIDSRDRIYASDPDWKARKGKFWRI
                     DTDGSTTLLDSLGTANGIEVSPDEKTLYVNAGRTVWAYDLSSGGDVSNKRVLIEFPDF
                     GMDGMRCDIKGNLYIARFGKGVVAIVSPEGNLLREVQLTGKRPTNVAFGGKDGKTLYV
                     TLQDQGNLESFRVETPGREWKMQKNVKPTK"
     gene            23295..24554
                     /locus_tag="PK288_01870"
     CDS             23295..24554
                     /locus_tag="PK288_01870"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HPM17691.1"
                     /translation="MKRVSVLIIMLFFVMAGAMKAQTYNIVIKGGHVIDPKNNINEVM
                     DVAVKDGKIAKLAKNIDAKEGIQVVNAQGLYVTPGLIDIHVHVYQGNYLNQQYMNGPN
                     CVTPDGFTFRVGVTTVVDAGCAGWRTFPDFKRQTIDRSQTRVLAMLNICGEGMRGDPF
                     EQNLKDMDAKLTGEFALKNSKDIVGIKLAHFSGPDWTPTDRAVEAGEIANMPVMIDFG
                     GSNPPLSIEELFFKHLRPGDIFTHCYASLGNSRESVVDEKTGKLKPFVLDAQKRGIVF
                     DIGYGGISFAYSQAIPAIKGGLLPNAISTDLHIGSMNAAMKDMLTTMSKCLAMGMDLQ
                     SVIRASTSDPARIIKREELGNLSEGSEADIAILNLRKGKFGLFDYTGYKIEADRKLEC
                     EMTIRAGRIVYDLNGIADPIVLARRPR"
     gene            24670..25125
                     /locus_tag="PK288_01875"
     CDS             24670..25125
                     /locus_tag="PK288_01875"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPM17692.1"
                     /translation="MKTERRTALKRMFTSLLGITGVGLAARAGTTEKEAGSISGFQQA
                     PLFSGHTKYGNLVFIAGKGAHVAPFDIKNHTEIVLKELEKELIKAGSSMEKVLKVNVY
                     LNDIADYKGMNEVYAGRFGMNPPVRTTVAVAKGGVPGDSLVEMDCIAYI"
     gene            25175..26398
                     /locus_tag="PK288_01880"
     CDS             25175..26398
                     /locus_tag="PK288_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17693.1"
                     /translation="MKRRDAIKNLSVLPVAGAVVGTTLSLESVTAAPAAPPAPKRNLF
                     RELGIRTFLNAAGTYTAMSGCIMQDEVIDTIRATAGEYAMIDEVQDKVGERIAAMCHA
                     EAATVTAGCWSALVLGTAGVLTGTDMEKVARLPHLEGMKSEVLVQKTHNHGYVHALTN
                     TGVRIVEVETADDVEKAAGPDTAMMWFLNYAGPDGKISDKEWVALGKKLNIPTMIDIA
                     ADATPVENLWKFNDMGFDLVCISGGKGLRGPQSAGILMGRKDLIAAARLNAPPRGGNI
                     GRGMKVNKEEILGMYVALEKFINLDHAKLWKTWEDRIAVMENSVKKIPGVSTSVIVPP
                     VANRTPTLIISWDKGRVKVGRDEFINRLRDGSPSVAVMGGKDESITITSWNLLPGQEK
                     LVAARLSEELTKASA"
     gene            26583..27827
                     /locus_tag="PK288_01885"
     CDS             26583..27827
                     /locus_tag="PK288_01885"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HPM17694.1"
                     /translation="MKHKLSLFILLQLLSAGFAAAQTYSIVIKGGHIIDPKNKINEVM
                     DLAIKDGKIAAVARNIDASSGNRVINAAGMFVTPGIIDMHVHVYYGTKMDQGLMNGPT
                     SVQPDAFSFRAGVTTVVDVGSSGWRSFPDFKRQTIDASRTRVLAFLNIAAEGMRGGPF
                     EQTTLDMNPVLTARCARQYPSDIVGIKLAHYNGHEWAPTDSAVRAGELAKLPVMIDFG
                     SATPPLSLEELFMKHLRPGDIFTHPYAYFPASRETVVDEDGKVKPFVFAAVKRGIKFD
                     LGHGGGSFRWSQAIPSVRQGFVADAISSDLHTGSMNSGMKDMANLMSKFLAMGLDIQE
                     VILRSTWNPAMIINRPDLGNLSIGSEADVAVFSLSRGDFGFLDISGEKLKGDKKFVAE
                     LTLRAGNIVWDLNGISAPDKKW"
     gene            27862..29682
                     /locus_tag="PK288_01890"
     CDS             27862..29682
                     /locus_tag="PK288_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPM17695.1"
                     /translation="MTVKNHFICIPGYTRNLLSRFIIPVIMAGAFAFTTAEGQALNDI
                     RIERHKPVPMSDGVKLYADIYLPSAPGRYPVLVTRTPYGVQRDGVHEMMVRFAQHGYA
                     VVVTDVRGRYESEGKWEPFRDEAKDGYETIEWAARQPFSNGKVATQGGSYVGHNQWAA
                     ASLNPPHLVAAFPALASTNIYANWLTMGGAFRLSFNYGWGVVRMPNRIMLPQYWHTEK
                     YMPENLKYDNVLMHLPLHNMDVTFEGAPVQHYRDWLTHESYDNYWKEISDEERFEKIT
                     VPVHTLGGWFDIFLMGTINGFVGMKKHGGSPEARAGARMIIGPWGHGPTRTFGGVDFT
                     PDAFMDMYDLQIRFYDYYLKGINTGLDFEKPVQLFYMGVNRWRSETDWPLPGTEYREM
                     YISSNGSANSVRGDGSLSFTKPVKKASDTYRYDPLNPVPTTGGNNCCGTPTAAGPVDQ
                     RPVERREDVLVYTSEFLKDTLTIAGPVKMKLFAATDGKDTDWMIKLTDVYPDGYSMPI
                     SEGILRARFREGIDKIRLLTPNQVYEYDIELTGTANAFLPGHRIRIDITSSNFPQFDR
                     NPNTGDPLGSSAVTRVAVQTIHHGGTNLSHVLLPVVKGVK"
     gene            complement(29691..31286)
                     /locus_tag="PK288_01895"
     CDS             complement(29691..31286)
                     /locus_tag="PK288_01895"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPM17696.1"
                     /translation="MKRRDFIKVSSAAGALGLVTPGIRVTGSPAGAPAEGFDLHPFIR
                     EHPEAVFINLTSVKEKTDATAIHDAAYKLAGEMFVRTSGGKGLPNSTRITCKPNWTCS
                     TGNKNDPEASLGITTDLNFIEGYLRGVKAKGPQNFFLRECACTFQWKPNGYIAMAERN
                     NFDLRDLTARDYWELGDDIIFRTVNGIVFREVAFMAPMTAEDTFLINLAKFKAHQMGI
                     TGAIKNLQGINAKRLHSFCAGSVDVFKTLDKRYHRFFQPDYLDRIAELHKKHIEAGFP
                     RWNEKIDRPPFNGGFWMEQWAQRMCDSFSVTPTGINIVEGIYGRDGDGFHGGPHNGKP
                     MDFMSNNIIFGKDAFRVDIIAHWLAGHEPGNFGLFHIGIERGLSDVLDPFDIPVYLWK
                     DGKATKARLDSFRRTPLLTYYLRKSSDGIDEPRYHMVDEPFDYKAWKSGKIAAAAQPS
                     IRALGTDAADKVVMEVGVPEKGDVYVDILNGNGELVWRMHADDLEPGVHQVVWDGFSQ
                     PGMYNMYVRGMGWDAEREMVIYT"
     gene            complement(31286..32881)
                     /locus_tag="PK288_01900"
     CDS             complement(31286..32881)
                     /locus_tag="PK288_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPM17697.1"
                     /translation="MKRRDFIRVSSAAGVAGLVGRGFESFSAPFEADGGFDLHPFIKA
                     HPEAVFINLTSVKEKTDSKAKYDAAFKLASEMFVKTKKGKGYPLSTKITCKPNWTASR
                     IDKKDPTANMGITTDIDFIEGYLNGVKAKGLREFYLRECAAPSSWVVNGYTPMAERNN
                     FDLKNLSTKDFWELGDEIIFKKIDGVVFKQAGYMAPMNAPDTFLINIAKFKTHQMGIT
                     GTIKNLQGITGKKFHQFCGGHYDIFKSYDSRYHQFFQPDYLARVAELHKKHVEMGIPR
                     WDTTMDRPPYGGGLWMEQWVQRMLDSYSVTPTGINIVEGIYGRDGDGFAGGPHEGGKA
                     MDIMSNNIIFGKDPFRVDIITHWLAGHEPGNFGLFHIGIERGFSDVLDPNDIPVYLWE
                     NGKAKKIKLESLKRTPLITLYLRRNANGENEDRYHMVDEPFDYNAWKGGKIARTEAPS
                     VKALGTDSNDKVVMEVTVPEKGNVYVDVLNKDGEIIWKLYADDLEPGVHQVVWDGFSS
                     PGLYTTYVKGMGWDAESEMVIYT"
     gene            complement(33086..34693)
                     /locus_tag="PK288_01905"
     CDS             complement(33086..34693)
                     /locus_tag="PK288_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17698.1"
                     /translation="MIRRDLIKNLSVLPLAGTFLPLESVVGPASGPLKTGPNIYESIG
                     VDPVINCVGTYTIIGGSLERPAVVEAAHAAAGHFVQYDELAFGIGRRLAEITGAEWGM
                     VSAGCAAGMKHVTAACVAGGNPEKMVRIPDLTGFDKTEVVIPKSSRNAYDHAIRNIGV
                     RIITVNTLEELGKALSPRTAMIYIMTGADTEPGREFAEDKIAALAKPLNIPFLVDAAA
                     EDLTVPNVHLQRGATVVAYSGGKALCGPQCAGLLLGSKAILMSAWQASSPHHGTGRDN
                     KVGKEEMMGMLAAVEDWVTRDHDEKMRTWHTYLENISKKVTGIKGIKTSIREPHSLNN
                     RSPMLAISWDPEQFNITGPEVGEILATTKPRIAVHSGFGDQEGSTSILISAGQMQPGN
                     DKTVADRIYEILNKKYPKPAPMKAPAADLSGRWAVDIEYYSSKSQHVFILEQDGNWIQ
                     GSHKGDFSLREMTGTIDGDQVKLFSTERMIADNVPFTFSGKLANDVISGEIYLGEYIR
                     ANFTAKRNGYKGTRRQIRYPKGQPLSS"
     gene            complement(34721..35992)
                     /locus_tag="PK288_01910"
     CDS             complement(34721..35992)
                     /locus_tag="PK288_01910"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HPM17699.1"
                     /translation="MLKKVIIPGLGKIIPVFVILLFQVMILHSQEIDILIKGGHVIDP
                     GNKIDGIMDVAVANGKILRVAASIPAGNAKKVIDAGGLYVTPGLIDIHTHVFVGTNNG
                     FADGFMCISPDDITLKAGITTVVDAGTSGWRNFPVFKRQVIDRSVTRVLAFLNIAGSG
                     MTGFPSEEDLNDMDARMTSLVIDQYPEIIVGVKIGHYRGSDWAPFDRALEAAKLSDVP
                     LIVECHMPQYPLEEILGRLRPGDIYTHSYCTAVDRDCLLDENLKIKPFVMEAKKKGVR
                     FDVGHGGGMFHFNIAVPAFRQGLKPDSFGSDLHRSSINSGMKNMLDIVSKFLNIGMSL
                     QDAVYSATWGPAGTLNRKDLGNLGEGAVADIVILSLKEGKFGFIDTRNHRLEGTKKLE
                     AEMTIRNGRIVWDQNGLSAENWLDHQKINQK"
     gene            complement(36390..37679)
                     /locus_tag="PK288_01915"
     CDS             complement(36390..37679)
                     /locus_tag="PK288_01915"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HPM17700.1"
                     /translation="MSGKTRIYAYRAIFLLAAMVFASGPVLSQQISILLKGGHVIDPK
                     NNINSRMDVAIADGKILRVAKDIPASNARRVVDVTGMYVTPGLIDMHVHAFHGTEPGS
                     YIADGWDALPPDGFTFRAGVTTVVDAGSAGWRNFRAFKSQTIDRSRTRILAFLNIVGT
                     GMYGRFEEQDVTDMNPVMTSYMITRLFPEILVGVKSAHYWGPDFTQVDKAVEAGKIAN
                     VPVMVDLGEHHPPLPIEELFMKHLRPGDIWTHTYANAPQDREVPVDENGKVKPFIFEA
                     QKRGIIFDVGHGGGAFHWKQAIPSVQQGFIADVISSDLHIGSMNSGMKDMANLLSKFL
                     NMGLSLNDVILRATWNPAKVIKRTELGHLSEGAPADVAVFTLRKGDFGFLDVRGTKMK
                     GTQKLEAELTIRDGRVVWDLNGIAAPMWDEVKPVSGR"
     gene            38157..39230
                     /locus_tag="PK288_01920"
     CDS             38157..39230
                     /locus_tag="PK288_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPM17701.1"
                     /translation="MNHNKLKLKSLAAGSKTFLSGGILLIILAALGFSSPAGQQAAKE
                     TGISAKKYSTVAVDEKNIKWFVTENGVVSFDGTKWKLHGSNDNLPSDVNHLALAFSAS
                     GPELLVASSNGATVASLPPDPQKGTRVFKASDSPSMGDKVSRVAAGSNLLKWFATDKG
                     IAAYGDGKWMKPEYTSYYPVELFEEFPITSLGTNAAGDTLYVGTYGAGVARIFKNNDL
                     DAISGASEYAIWGPIIMPSDTIQSIYIARDGSKWFGTNLGIAQHTGSNTLDNWTVYTT
                     EEGLVNDDVQAITADLKGNYWFGTKGGISVFNGSSWTTYTRSNGLNSDNILCLGVDKD
                     GVVWIGTDNGVNSFRDGQFTSYR"
     gene            complement(39388..40623)
                     /locus_tag="PK288_01925"
     CDS             complement(39388..40623)
                     /locus_tag="PK288_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPM17702.1"
                     /translation="MKKNIMIILSGLAIFLLAVCCSKENNEEPKLTPKNITLSAAAPI
                     AIQSSNEFGIELFTRVTESENKNLMLSPLSASTALTMLLNGCDGDTYSQLQGTLKYPA
                     EMSISEINEAYKSLVGQLLTVDPRVQLALANAIFYLDGFDVKPPFLATMSDDYKATVK
                     ALDFAAPTALTTINKWASDNTKGKIPEVLSEIPGDEVMFIMNALYFNGDWSCQFDKSM
                     TQELPFLPDGGSATDVPTMKGKVGAKFACGNGFRAIEMPYGQTNFTMVVIVPNETLSA
                     FYPSFTYQVWDKLCSDLDELHEFDKITVIMPKFKFSYEKYLNNQLMSMGMIDAFSSSD
                     ADLSGISDSEIWVDYVKQNTFVEVDEEGTEAAAVTTIAVGVTSPGPFNIYKSFIFAIR
                     ERTTNTLLFIGQVVNPEQD"
     gene            complement(40808..>41140)
                     /locus_tag="PK288_01930"
     CDS             complement(40808..>41140)
                     /locus_tag="PK288_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPM17703.1"
                     /translation="RITLVMDNYNTHGASALYETFRPEEAKRLWDRFEFVYTPKHGSW
                     LNMAEIELHVLNGQCLNRHIPAMDKIKEEVAAWESNRNNKKCKINWQFTNKQARVKLK
                     KLYPSIYD"
BASE COUNT        10648 a         9841 c         9673 g        10978 t
ORIGIN      
        1 ccccagctag gacttgaacc taggaccccc tgattaacag tcaggtgctc taaccgactg
       61 agctactgag gaataaatac ttatctcact ttggtcggac tgatctcgca acaataccgg
      121 gttaccgggt gagccctgat cttccgagac gctgctatcc tgatttttaa aacctgtgcc
      181 gtttcgcgtc gcaaaagtaa gggcttttac tgaattaagc aatatcttta aagaaaaatt
      241 tatatgaagc ggattctggg tattggcaac gcacttgtag atattctgat tctaattgat
      301 gacgacggaa tacttgaggc ctttgatctg ccgaaaggca gcatgcagct tgttgacatt
      361 aagaagtcag agcagataca ggataataca aggaatttca gtcagacacg ctcgtcggga
      421 ggttcggttg caaacaccat ccatgggctt gcaatgttag gaattgatgc cggttacata
      481 ggatccacgg gcaaggatgc cacaggtgat ttctttgaga acgacctgaa agcagccggg
      541 gttaaaacat atctgacaag acggaattca gttaccggaa ctgccctcac tcttatttcc
      601 cccgattcgg aaagaacatt tgcaactcat ctcggagcgg cggttgaact tcatccgggt
      661 gatcttgatc cggcctggtt ccgtgaacac gatattctct atgtcgaggg atatctcatt
      721 actgacgctg ctcttgttga aaaagcctgt ctgctggcca gggaaagcaa catgacaatc
      781 gcacttgatc tggctagtta caatgtagtg gaagcctatc gggattcgtt caaaaaaata
      841 gccgacaggt atgttgacat actttttgcc aatgaaatgg aagccgaggc gtttacgggt
      901 gttccttcaa tgcaggctct tgatctgatg ggcaattcag tggaacttgt ggtcatcaag
      961 gccggagcaa acggatcgtg gctcagaaag ggcaagaaaa caattaagat cgatgcaact
     1021 cctgtcaggt gcgctgatac aacaggagcc ggtgatcttt atgcttcggg gtttctttat
     1081 ggatatgcaa ccgggatgga tcttgaaaaa tgcgggctga tcggctcaat gatggctgaa
     1141 aaagtgatcg ggataacggg tgcacgaatg agcaaaacgg agtttacaga gatcattaat
     1201 aacataggga aaatagttca gggtccctga atcaccgatt tgccgggcta ttgtcataaa
     1261 ccgccccgtt tgtcaggaag atgcagccag tccgggggtt tttaccctgt atccgctgat
     1321 cccgtaatac actatgaagg cgaagcagac cagcgggacg gtaaatccga gggccatgtt
     1381 ggttacatcc gcaatgcgtc ccataagtat aggtatcagc gcccctccga ctatcgacat
     1441 gacgagcagc gatgaacctt ttttcgtaag tgggccaagt tccttaatgc caagggcaaa
     1501 aatagtaggg aacatcaccg acatgaaaaa ataggtggta aaaagcgcaa caaccgatgt
     1561 ccagcccaac cccgcaacga ccaggatcat ggttatcaca ttggctgagg catagaatgc
     1621 aagcagcctg tttggcctta tcaggcgcat gaatatggta cttccggtaa acctgccaag
     1681 ccagaacaat cccatgcctc cgaatccaag gatctgcgct gcctgctggt tgctaagggt
     1741 tggtatctcc tcggtaacgt aatttataaa aaagctgttt actccggttt gcgccgccac
     1801 ataaagaaac tgagctatga cggcgaggac aaaatgggga tatttaaaca gatccttaag
     1861 cctgcctcct gattcctgac caggatcgcc ggtttgcata tcttccttga gttttgggaa
     1921 cttaaccctc cagaaaaaga ctgcaacgat aagtaccagg gtcccgataa acatataggg
     1981 aagggcgatt gttgcaaact tgttggagtt gccgttaccg ttagccgaaa agataagcat
     2041 tcctcccacc agggggccgg caatccatcc gagaccgttg aaggactgtg acagattaat
     2101 ccgtctttct ccgtattcag gtggtcccaa aacagttgtg tagggatttg cagcagtttc
     2161 gaggcaggtg aggccgcagg caatgacaaa caggcagaac aggaaacttc cgaatgtctg
     2221 gatgaatgta gcgggaagca tcaaaaaagc cccgcatgca aaaaccagca atccgaaaat
     2281 tattccgcgt ttgtatccga ccctttgcat taaaaggcca gccggtattg ccatgaagaa
     2341 atatcctccg taaaaaacaa actgaaccag tgctgatttg gctttggaca tgttcaaaag
     2401 ttcctgaaaa tgcttgttaa gcacatccag acagccatga gcaaaccccc agagaaagaa
     2461 taggctggag atcagaataa agggtacaag aaacccttca taaacaagct tctgcctgtt
     2521 tacgccgacg gaaagatcat cattcattta caacttgatt taggcagcac aagattgtca
     2581 tgatttctga cttttccaat attttcctga aaataaaatt cccgctgagc atacagtctg
     2641 actaataaat atatccttca gacagctatg tatagtcagc gggaagagta aaacggtctc
     2701 agatcatgct tttttaacca ggaggttaac cttgagtttg aattcatcgt agatgatctt
     2761 atctccaagg ttgtcgaaaa atgaaccgga cccatacctt acattgtatt ttgaacggtc
     2821 aacaacaata tttgcaaagt accacagtcc atcatctgat ttctgcaggg atgccttgaa
     2881 ttcaaccgga tgggttatat ccttgatggt caggttcccg ttgacaacac cggtcccttt
     2941 ggcaaaagag gtactgcctg ttattacaag ttttgaaaca gggtattttt caaccccgaa
     3001 gaagtcgtct gatttaagat gtccttcaag tttctggctt ttgtctgcat cctgaatgct
     3061 ggccatgtct ataagaaatt cgcctccggt aatcttatca ccatcccatg ctatccagcc
     3121 tgagtgaaga ttaattgttc cggaatgctg cccggcaact ttctcgccga gccattcgag
     3181 ttttgtcctg gtcttatcgg caatccactt ttccattgaa tgaagattca cggcaagcag
     3241 tgataaaaaa ataactagtg tgattgtctt tttcatgttc ttttgtttta aatacatgca
     3301 ttaaacaaaa aacaggaaag actgttcagt taggcattat atgaatggat aataagaggg
     3361 atcagagcct gtgtatattc aggccgccgc tgatttcaaa ggtcatgccc gtggaaaaat
     3421 cccagtcgcc ccgtgcaatc gaggcaacgc cttttgccac atcgtctggc agaccccatc
     3481 tgttttgagg aaccaggcca tctccgataa gcctgtcgta cttgtcgcgg gtagccgcag
     3541 tcatgtctgt ctggattatc cccggccgta tctcaaatac gaggatgttt tcactggtta
     3601 gcctgtcgga gaaaatagca gaagccatgc tcagcccggc cttggaaatg cagtactcgg
     3661 ccctgttcac agatgtaaga acggcggaaa ctgaggttat gaatattatt atcggccggt
     3721 agtgttcaat ctgttctttt atccagatca tttccctggc aactttctgc gcaaagaaga
     3781 ccgggccctt caggtttatg ttcattaccc tgtcataact ttcactgctc atttcaagta
     3841 catcatgccg ttgaagcgga gccaccccgg cattgttcac aagaatatcg atccgcccgt
     3901 atctctcaag gatatttgaa acaacctctt tatgatattt tatacaggaa atatcaaggc
     3961 cgaccggaaa aaactgggct cccctgcttt caatttcagg ctccagtgtt ttcattcctt
     4021 cgctgtcaac cgacctggca atggcagcaa tatcataacc ttctccggca agggcaacag
     4081 caaccgatct tcctatacct ctgctggctc cggttattac ggctactggt ttaccgttca
     4141 tatttgcaag ttaattcagg ttagaagaaa actggtgaca aaggtaacaa tttgctcagt
     4201 ttgtttaatc ctgcaagaaa atatcttcac ggaattcttt ccagactttt tcaaaccaga
     4261 ggttctgttc cggtatcatt ctcaccggtg caaggttggt attgattttc tctccgtcgt
     4321 atctgatctc aattattttt tcctcaatat cacgatcata gctgcaaaga atcggaaact
     4381 gtgaatcatt ccccgggtct gttcctgcag attccttcgt ttcatcaaca ataaatgatc
     4441 cgcggctgcg cccgccgccg gcaatatatg ttctgatcgc ctccaggtaa accaggtgtg
     4501 ccaggcagct gttcgagccc ataaagcatt ctgcaagctc tttcaccgat gtagccgcgc
     4561 agagcttatc gaaatcatca tacattatcc gggcctcttc cacggccttc attatctttg
     4621 ccccgctgcg cagtatactt ccattatccg tcattcgctt tctgatcacc ttcattgcct
     4681 gttccggcgt ggagccgtga tatactcccg agagccagtc agctgccctt ccgaaatcct
     4741 tttcaattgc tggaaccgtt tcgctcagga acacactctt ctccggaggc ggcgtttgat
     4801 atcttttagt gataaagaag gctgcccgat gtcccccaac ctgtcccgaa ttcagggatg
     4861 atccgcccgg gcggtacaca ccgtgggagc cattcacttc gcccacgggg aaaagatgcc
     4921 tgagatctga ctcccaccag atattgcctt tcagtccccc gttgcaatgc tgggcgcaga
     4981 tcccgatttc cagggcttca gctctaatat caataccgtg atccatgtaa aggctgattg
     5041 ccggtgaatt gagggcttca agcctttcaa gcgggagcgg tttcagtgag tcagaatatg
     5101 caagatagtt atacacttca ggatcaagtt cttctggctt gaaagcctgg catccgtccc
     5161 atgtaaggtt tctcatgaaa tcgataaaca ctctcctgcc tttttccctt tccctgaaaa
     5221 ccagaagatc gataagggat gaaccatacc cggatatttt ccgtacatca aacggccact
     5281 gataaccttt cagaaagatg gccctggtga tggacctgta atcggggaag aagtcgttaa
     5341 ggaattcccg cggatcgctg ccatccgggg ctgttgaata ataccgcggc agaacctgct
     5401 gatagctgcc tgacaggttc cagcggaatt tcagcgaagc aatgccgaac tgggattcgg
     5461 taagattctg ccctgatgcg ccggctttaa atgccattcc cacggagcct gtctgcgact
     5521 cggggtacac gcttgtttca tacatgtcgc cggggccccc ggttccaagg atgatatttg
     5581 aagcattaaa cactgtgaat gcattaacag ggtcgttctc caatatattc acggcaagcg
     5641 ctcccacaac cctggttttt tccctgtcgg taagcagtga aatcacataa tgcctgtcga
     5701 ggatcgtgat ttttctcctt aaaacttctt cggaaaggac cctgaacatc attttcgatg
     5761 tgtagggacc agcagaagtg gcccttgcac gcggatcatg gtcagttttg tatccggcgt
     5821 atgaaccgta tttgtcatga gggaacggta cacccatgtt aacaagattc ataaatgccc
     5881 tgaccgatcc ctgggcttca cacagcgcaa tatcgccatg catgcatttt cccctgaaca
     5941 ggtcgcgggc aaggtcagta acggaatcag gaacatcacc tgcaagcgac agcttgtaat
     6001 aagtctgctt gtccgatccg gcattgttgg aagtgcccca gccccacccg gccgttgcaa
     6061 taatgatatc cggctctccc attgaatgca ggcttacggc agcattgagg gctgccgcac
     6121 cgctgccaat gatgagggtt ttagtactgc gatagttaac agaaaaacca ttcagccgta
     6181 taacttcatt ttccatcagc aatcagtttt ttcagctcct ttccatatat ttccttatcg
     6241 cggataacac atccgccaat atgaaggttt cttataagac gagtgcatcc cgagcagcaa
     6301 atgcaggttt tggaagggtc ggccctgccg gttctcataa gatcgagggg cattgatgga
     6361 tatgcaaatg agtttcttcc gaatcctgca agggatacat ccctgctttt aacaagggcc
     6421 gctcccacat tcggtgcata ctgacgcaga taggaaaaag ctgttccggc aataaccaga
     6481 tccggaaatt ttttctgaaa aaccgatgtg gcatttatca ttctcatcac cccctgcaag
     6541 ggatgctcat cggggacctt cattccggga agcggaacat cgtatggcct tgagatgtga
     6601 ggattgaaat aggggcttcc cattgtaatg ttcagcaatt caattcccct gtccattaac
     6661 tgtcccacaa gcatcatagc ctcggtaaaa tcaggctgat ccatatcatc caccccgaag
     6721 ccgcccctgt acatgtcgga aatgccgagc cggctcgtga ccatcagtcc gggaacttca
     6781 tatcttatcc tgtcaaaggt ttccaggaag aacctgaatc tcttttcagg ttcttctccc
     6841 ccgtaaatgc tgttcctcct gtttttagcc gccagaagct ctatcataag ataaccatgg
     6901 catgctttga tatccaaagc attgaaaccg gcatcgcggg caagtcttgc ggcaattata
     6961 tactgatcct ggatccttct gagatcgtcg tcggtcaata tttccggagt tcccctgtcg
     7021 agcaatggat tgggggccgc tatccggggt gcaggttttc catcgggttt gctgtaacgg
     7081 ccggagtgag tgatctgaat aaccaggaat ggatccctgt tttccagtcc gctgttttta
     7141 cggatccttt cgttcagaaa ggcaaactta tccctgcttt tcctgtttat ccagagctgg
     7201 cggggattgg acctgccttc atgatttacc gcaacagctt caaaccagat agttccgctg
     7261 cctccggcag aataccgcaa ataacgtctt tccgtaagtt ccgagggagc tccttcatta
     7321 tcggaatcat atccttccat gggctggacc actatccggt tagggataat aatctctcct
     7381 gcttttgcac tttccagcaa cggggaaacg tcatctgaaa aaggcagggt aaagcccagt
     7441 tcggatgcct tacggataag ctcatctttt gtcctgtaat ggaatctttc gtgcatgatg
     7501 ggagaatggg tgaaaatggt aaaaatacac gaaagaaagt tgtccccaaa ggagaaattg
     7561 atattaacgt cagctaaaag taaccgctgc caaaacgcgg attaaccacg ttgttgtata
     7621 tcgatccgaa tgtataggaa agacttaccc ctccctggac aaaatagctg gaagcaagct
     7681 gtttcagctg caggagacgg tcggcttcag aaatttctcc ctttctgaga ttgggctgaa
     7741 aattattgta ggaaacgctt ccgttaactg aaagggataa tcctttcagc accctcagcc
     7801 tgatatatcc ccaaaggttc accctgtttt tggataagtc ggacagatag ttggtttcag
     7861 aaagagaaat atttattgat ccccattttt cctggatctg gtatgcaacc ataagtgaat
     7921 gcttgaagaa attatcatac agtttctcgt atatggttga atcaatataa ttgctgtact
     7981 gatatccaat cgcatacagt aaccgcaact gtttatgggt ggcctctgaa tacggataaa
     8041 gatcatattc tatggcaggc attatttcgg tcgtaaagtt tatgttcgct tcggttgatg
     8101 tgcggttttc ccagcgtatt cccgtggata agtgatttcc gagacttttc acaaccagca
     8161 gatgcagtga attattactc tttgaataag ttgtatccgt ttccgaatca ataaatcttt
     8221 gcttgttaat gttctgattc agatttgcct ctattttcag atcaggagta atccttgaaa
     8281 tttcaagggc attccagatc tgaaatcgtt ttgcagattc ctcggctgaa tatctcggac
     8341 ttgaagacag ctcgaacacc cagttatccc agcgatcctt tacatcctca agttcaagtc
     8401 ccggttcatg tcctatcgta atttcatcaa tcagcggtgt ctttgcaata taccgcatca
     8461 ggcctatttt gagaatggtt gttctttttt cccttataac cgggccggtc tggtcaggaa
     8521 ctgttgcaaa tgtaatggta tcattcattc ctgaaaaatt cttctgtccc tggaatgtat
     8581 aggtgtacag cgttcctccg cttccggtgg gctgatcggt cacattgaca tatacctggg
     8641 cttcctttac atccctgaca taattcacat atggaacgtg ctgacggata taattcatat
     8701 cgcagttccg gcagtcgaga aataccttaa cggccccttc ccgcgaatga ttactccggg
     8761 ttgcagccgt atcggagctg acaacaaccg gtccggtttg cagttcatta tcctgggcct
     8821 ccagaaccag agaagcactg atcaatatta taaaaaccga taattttcca attttattaa
     8881 tggtcatagt tggtcagttt acatactact ccccgaattc gccggccgga tggaaagatc
     8941 aggcctccca agcagaactg caaatatcat aatatatttt atcagaacaa cttattggta
     9001 tcaccacagg cgaattaatt agcttttttt aacattcgtc ccggataaag cactgatctc
     9061 ggaccaatcc atgattgtct cagggctgct cctgcataat atcccagaag ccttaaaaat
     9121 atcctggtcc ggagaaacag ggcgggaacg aagaaaaaga tgagacctgc cgctgccaga
     9181 atgacgcctg agattatatt gtttacaaca agaaagatct gtgtcagaac tccttcggca
     9241 cctttaatat tccttgaaac atagagatgt cttgagatct gaacctcggt tttggtgatt
     9301 gcggcaattc ttttgtttac ccgggaactt ccgccatgat tgtgaataat cgttacatta
     9361 ttcagcctga caacttttcc catttccctg tttattctgc ggcatatgtc aacatcttca
     9421 taatacatcc agaatccctc gtaaaaacca ccgagctgaa gataaagatc tcgtttcatc
     9481 cataccactg acccggagat ccagtcggga aacgtcacat ggccattatc ggtgacaggt
     9541 gcagcagttt ttctgaaaat cctggcgact gaacgctgga atccggtgag gtttgaaaat
     9601 tcaggaaaat cacccgtagc agtagtttct ttccccctgt catttacctg cctgcatgat
     9661 atgatactgt actcaggatg tgcgcgggct tcttccagca tcctggctat ctcattttcg
     9721 tcagcctcgg tatccgggtt gagaataaga acatactcac ctgaggcgtt tctgcacccg
     9781 agattattac cgttggcgta cccgccattg acagggttta aaatgaacct gaaatcaggg
     9841 tattttctta caaaatcgtg aataacctgg tcacccgaat tattatccac cacaataacc
     9901 tcgtaactaa aattgtctcc cctgaatccc gccagggaat caaggcattt ccgaagcctc
     9961 gcccagcccc ggaagcatac aacgattaca gaaatatcaa tcatattgtg tccgaccttg
    10021 ttcctgataa aacaacattt ctttcatcat ccgggccccg tctcacactt ttttcccgtc
    10081 ttctgtttca ggcccatacg tatctgaaat cctgtcagat ccgtcctcct catcatccat
    10141 ccacacggga accgggtcct gcggtccctc attcctgaag gcaacggcaa gcacaatccc
    10201 cgaaataaag cccagcatat gggattccca cgagatattt tttctgacaa catcgggaaa
    10261 tataccccaa accattgaac cgtaaagaaa tactatcagc agcgacagcg caacgagcct
    10321 gaagtatttc cgtataatac cactgaagaa aaggaatgag gcgagtccgt agacgagtcc
    10381 cgaagcgcct atgtgccacg atgtcctgcc tgccagccat acaaacgatc ctgtcaggaa
    10441 ccaagtccac agagatattt tcactgcaat ctccgaataa aaatagaaca gggcagttgc
    10501 caggaaaaac aggggaaggg tattattgaa cagatgttca aaatcggaat gaatgaaagg
    10561 ggacagcaca atgccgggca agcctctgac ggaaagcggc aggatgccaa gatgggtaaa
    10621 gtcaagatca aaaaggacct cagccgtttt caagatccac atgagggaaa tgataacaag
    10681 tggaattatc atgctcagga gcattttttt cctgtgcagc aaggcttctc cttcagttat
    10741 gttcagcccc gggaattgca ttattgccag taacagtgct gcattatttc tccctgtccg
    10801 ctaatcctgc ggcaatctta ttcctgcatc atgtcgtatc ctgtcgaggg ttctgatcag
    10861 gttgcggctg aatgagaggg gaacaaccgg gctttcagtc aggccatcgt caagacagcg
    10921 catcacttcc ccggcctccc agtgatatcc cattgcaggc gggttgaata catacgtttc
    10981 cttctccata tcgtcaggct ccaggatcac agtctgattc atatcccggc cgcgggaaag
    11041 agaaatcctg cccctttcgc ataaaatcac gcagccgatt cctgcattgg tccggaaaga
    11101 actgtaaatg ctggccatgc gtccgtcatt atatctgaaa atagcactta ccgattcttc
    11161 cgcccccgtc tttccgaatg aagcgtaagc cttgatcccg tcgggaacac cggcaaaagt
    11221 gagagcgtca atcaccggat aaatacctat atcaagaagc gatcctccac caagctccgg
    11281 gttgaacttc ctgtcgtcag gatggaaagg agggttaaag gagaaaaagc attccatatg
    11341 aagaagccgg ccgacaatgc cggattcgag tatctccgaa gctttcctgt aaaagggctg
    11401 gaagggaggc cagagtgcct ccatcaggaa gaccttatta cgggaagcct ctgaaatcat
    11461 ctcttcaacc tcacgctcgt tgatcgcaaa cgccttttca cagagtactg cctttccgtt
    11521 tttcatgcag agtattgcct gctccctatg atgcgaatgc ggtgatgcaa tataaactat
    11581 atcaacccct ggatcctcag ccagttccct gtaactgcca tagaatttcc tgaacccgtt
    11641 gtctgctgca aacttttccg cccgccgaag gtcacgcgaa ccaactgcat acaactccgc
    11701 attatccaga tgcttcaatg cattcgtgaa ttttccggcc attttgcccg gggcaagtat
    11761 tccccattta taccgttttt ccataaccaa agtttgtata aaaatagaca ttctggtcat
    11821 aatggcaact acccggtaaa aaccggggaa ttatcatttt cagtactaac atggaaatca
    11881 gcaacgattt tatgttaagc caatgcttcg gggtgaatca cccgttttcg cggcgatttt
    11941 tcatgtgaaa ataaattcat atactactga agattaagtg gctcacatgg aacccgcatg
    12001 aatcttattt tcctgcttgt ttgtgatctt ttatcctgtg ggtttcatat tattttaaac
    12061 tcaaaattcc agtcatcatt aataatctta attccacatc gcttattatc tgatgtttac
    12121 tgaattattt ttatgtcttt aataattttc agtactatgt attgcatagt atattatttt
    12181 atatatatct ttgcaataaa ctgtagtatg caaattccag tagtggtttt tacaaggttc
    12241 tttaaatatc atcatacctc tacaggagag tatttattaa gttcaatctg aatttgcagt
    12301 caataattta catctcggaa aatgaaaatc acggtcagta ttaatcttgg agggtattcg
    12361 ttcaatattg acgaggatgc ctataccgaa ctcaaaagat atctgaaaag tcttgagttt
    12421 aacctggcag gggaagagag ttccgcagaa atcatatcag atatagaggc aaggatggcg
    12481 gaattgttca gggcaaggct caccgggtac aaacaggtga ttactatcac ggatgtcagg
    12541 gatgtaattg ccgtaatggg gaatccggag gacatatccg acaaggaagg tatgtcgtca
    12601 ggggcgaaat tcacggagcc cggggctcac aggatgtacc gtgatcccga ccggaggatc
    12661 attggcggag tgtgcgcagg gattggagct ttctggcaga tcgaaccgtg gattgtgcgc
    12721 gtaatattta ttgcggtcgc catggccgga ggactcggca tcctggttta tctcattctt
    12781 tacattgttc ttcccgaagc caggaccact gcccagaaga ttgaaatgaa aggggatccg
    12841 gtcaatattc acaatattaa ggaagcggtg aaaagagaat ttgataatgt gagaaagaac
    12901 atgaatctct gatggtcagt tgttcctgaa aacaataaat cttataaata tggatatcga
    12961 aaatacaaaa gcccagatgc gtaaagggat tctcgaatac tgcattttat cggtattatc
    13021 gaaggcatcc tgttatgcaa gcgatataat cagggagctg agggaggcgg aattgatcgt
    13081 tgtggaagga actctctatc ctttattgac aagacagaaa aatgccgggc tgctgagcta
    13141 ccggtgggag gaatcgcaac agggcccgcc caggaaatat tatgaactca cttccgaagg
    13201 cagggagtat ctggccggac ttgacaaatc atgggatgaa cttgttgaat cggtaaggct
    13261 gataagaaac cgttgaatcg ttgctaacct gaaaaactga atataatgaa cagaaccata
    13321 aaaatcaatc ttggcgggat actgtttcag atcgagggag aggcatacaa tatgctgcgc
    13381 ggatatctta atgagattga cgccaggata ggaaaacttc ccgatggtgc cgagaccctg
    13441 gaagacattg aggcccggat tgcagagatc tttcagtcgc agggtgcagc ggccggagtc
    13501 atttccgcag ataatgtcgg ggaaatgata aaaatcatcg gcaggccgga ggagttcgaa
    13561 acaggcgatg gtcaggaggg cagggccagg gaacatacaa gggtgccccg cagccggaca
    13621 ctctacagga atcccggcga ttccataata ggcggtgtct gcgggggact gggctcatat
    13681 cttaatattg acccggtgtg gatcagggtg atcttcatag tctttgcttt tttcgccggg
    13741 gtcgggttct tcgtttatct tgcgctctgg atagcactgc cgatagcaga ccccgaaaca
    13801 attaaaaggg aatcgtacat gaaaggtgct gatcatgcag atttcggctt ggaaaagagg
    13861 tccgtcgccg gcaccaccgc ctcccgtcat cctgaaaatg cctgccctga acgtctggcc
    13921 ggcgcactga atgagattct cagggcaggc gggaaaatgc tcttcattgc gttgaggata
    13981 ttcatggttc tcctggggct ggcaatcctt ctttcaggct tcctcgcact gcttggattg
    14041 atcatggtgt tcatcttcag atttccgggt atttattctc cccatgccca tgggctgaat
    14101 ttctcctggc tattcgattt cctgaaatat gccgtcaatc ccgcaacctt cccatggata
    14161 ctgaccctga cattcattgt gatgctcatt cctctgctgg caataattta cctgggtatt
    14221 aaaatgattt tctggttcag ggccaggaac gggatgattt cactcgcagg gtttctcatc
    14281 tggctggtct gtctggctgc attatcggtc attttattca acgaaggcat aggctatgct
    14341 gaaacagcaa aaaaggacgc cggggaagtt ctgaaaaaaa cgacgggaaa catttacatc
    14401 ttaagcggca ataaaataaa tgatatcaga tatgagaggg aaatctcatt tccattcaga
    14461 ttttaccgca tgtattttac ggatgatctc agcaatccct gcattactcc ttccctggta
    14521 atcggcagat cgaatgatga ttacattaag atcaatgtca ggaaacgttc ttcgggaaag
    14581 accaggtctg aggccgcagg caggatggag cagcttgctt acaattattc cgttgagggc
    14641 gacacgattt tcctcgacga gtatttcaga atcccggaag aaagaaaatg gtcattcgac
    14701 aacattgtgg taagtctgta catccctgaa ggaacaagga tctgcttcag ccggggtacc
    14761 gaaaacatgc tccgcactga ttggagctgc aattacggct gcagcaaacc ggaaacatta
    14821 agggaatacg gagcaaaaga atccgggaag cctgaatctg aatggataat gacggaagac
    14881 ggactgagaa gtaggtccct ggggcagtga tccggataat tacttctctt ttccggtcgt
    14941 gcatttttat gaaagggcgg cggctgctcc ggcaatgatc cccaggttcc ggccggataa
    15001 ccggtttgat atcctgccct gatatccacc tgaaagctca gatatgcaaa ggttaaatga
    15061 ctcattcact gtggtcttct gcacttcacc gggaagtcag tgcaataaag gaaccacacg
    15121 agccgaattt ctattacctt tgagaatgat ctgactaaaa caaatcctat gaaaacgcat
    15181 aaaggttatt tacttctgcc tgttgtagcg gcactggcta tcgtttcctg tcagccgggc
    15241 aggaaagcgg ccgttcccga agatctcacc cgatatgtcg atccgctgat cggcaccgca
    15301 cactgcaggt ggttccatgt tgcccccggt gcaagtccgt tcggaatggc caaacccggt
    15361 ccctcaaccg acgggcacct tggaaattta agcggatggg aggcagttgg ctatgatttc
    15421 aggcacaagt cgattgaagg ttttcccaat ttccatgaat tccaggttgg cggggttgtt
    15481 ttcatgccaa cgacaggtga gttaaggacc gttccgggcg atgttgactc agcttcagga
    15541 ggttatcggt cattttttga caagaaagat gaaatcctgg agccaggtta ctattccgta
    15601 attctgaaag actatggcat aaaggctgag cttacatcaa ctcccagggt cagttttcac
    15661 cgctacacat ttcctgcagg cgaccggtcg aatattattt ttgatatcgg caacaggcag
    15721 ggtgaaagcg gtgaagtgac ggatgcccgg gtctggttaa gtgatgacgg gcgggttgag
    15781 ggatttgttg agacataccc ggaatatgta aagaagtacc agacgggagc cagtgtaaag
    15841 atgttctttt cggccattgc tgataagtcg cccgtgtcgt ggggtgtatt caacggcccg
    15901 gaagtaaggc atggagagaa ggaaacttcg ggccgcggag ccgggttgta tcttacctgc
    15961 aaaaccaccg ggaatgaaag catcacaata aaggcaggat tatcatacac atcagttgag
    16021 aatgcgcgcc tgaatcttga aactgaagca aaggatatgg gttttgatga ggcccgggag
    16081 aaagcccgcg atgcctggaa ctcatatctt ggcaggataa gggttgaagg cggcagtgcc
    16141 gcagacagga tcaagtttta caccgggctt tatcatgctt tgcttggccg ggggctggca
    16201 agcgacgtta acggggcata tccgaagcat gatggaagca ttgggcagat acctgccggg
    16261 ccagacaata agccgcagtt taatttttac aacaccgatg ccatctgggg cggtcagtgg
    16321 aacctgattc agctctgggc ccttgcatat cccgaatatc ttactgattt tgtaaagacg
    16381 catctcaccg tttaccagga tgccggatgg cttggcgacg gtcttgcctg cagcaggtac
    16441 gtatcgggtg ttggcaccaa ccaggtgccc ctggcgattg ctgcggcgta ccagtgcggt
    16501 ataagggatt ttgatattca gaaaggctat gaagcttccc ttaaaaatga aataggttca
    16561 gagaatcgtc ccttcggggc cgggaaggca gatgtgggaa agttcgttaa atacgggtat
    16621 gttccctatc ttgcaaaggg ggagggaccc gatgaacgct ggaaattctc atgttcccat
    16681 acccttgagt attccttcac gtcctttgct gtggcccaga tggcaaaatc actcggcagg
    16741 gaagaagatt acaggcttct taccgatctt tcgcatggct gggaacggat cttccatccg
    16801 cagcggaaac tgatgtggcc gaagcttgag aacggaaagt tcgtcgagaa tttcgatgct
    16861 gcggtgcctc acgacggctt ccaggaaggc aacgccttcc agtattcgtt ctatgtcccg
    16921 cacgatcctg agggtattgt tgccaaaatc ggcagggaag aattcaacag ccgcctcgac
    16981 agtacatttg ttatttcacg aaaagacatt ttcggagggg gtaccgagat acacgcattc
    17041 gccgggatca caaagcctta taatcatggc aaccagccat gcctgcatac ttcctggctt
    17101 ttcaaccact caggaaggcc ctcaaaaaca cagatgtggg taagggcaat actgaatgat
    17161 ttttacggaa cggagggtat ccatggttat ggctacggac aggacgagga ccagggacag
    17221 ctcggggcat ggtatgtcat gtcggcgatg gggctttttg atgttaaggg attaacagat
    17281 accgatcctt cattcggtat aggcagcccg ctgtttgaca gggtgactgt tactctgaac
    17341 aacaggtatt tcaaaggcag ggagttcgtt atcgaaacag aaaacaattc tgctgataac
    17401 ccctatatcc aggaagtttc agtgaatgga aaaatattgt cctcggtttt cgtgcctttc
    17461 accgacatta ccggcggagg gaagatggtg atcaaacttg ggaacactcc aaaagacctg
    17521 ccatagaatg aaatccggcc agaattatcc tgaaccggcc tgggcaagac ttgtttcgct
    17581 tttctgccac ccgtttctta caataccgct ttttgtggca ataatgatgt tcgccttcaa
    17641 tgacgtctgg gaagccgcaa acagcatttg gctccattaa tggtccaacg gaaaacatcc
    17701 cggttatttc tgtcttaatc ttattgcggg atgaagggtt tccttttctg taccttcgtt
    17761 attccggcca gcattatggc attgcttttc atcggttctg taactacctt tacgtaatcc
    17821 tgctgcaggg tctggtaatt ggttgttccg caaaggttta tttctttgat caccttcccg
    17881 aacctggtgt ttgtccattc gaaggcaaaa aaggtaacag ggggataccg gccctcttcc
    17941 gaacatggca ccgggtcggc catatagcaa taggcactct gcggcgagcc tgtattgccc
    18001 tggagccggt caatgctctt ttccccgccc gggttccatt ccaggatatt gaccccgtac
    18061 tgaacaggaa tgatctcctt aaagccatcc tcataaatga tctcatacca tccgaggaga
    18121 tccgccgaat cgaaaaagtt gggaatattg aaataggctt tctggttagc ggcgggcaag
    18181 gcgcatgcat gcaggaatat gaggctgctt acatcctcat ctatgacgat cccgttaacc
    18241 tggtcgggca ggtggacggg accggttccg gcagaaccca ctgcaacgag catattgccg
    18301 taagtgccgg caggattgac catcctgaaa taaagcggac ccttttttat ttcgtctgtc
    18361 aacagcccgc gcagatccat gttgaatgct tctgtgccgg cggtcatgtt ggcatattgc
    18421 gaaatatcca caggcatcag gggatcaccg tcccttcccg gtattctggc accatgcaga
    18481 tcctccctta ccgtactcat cgcctccctt acaaccgacc ccagctccgg caggctgatg
    18541 ccatgcggtg accataacag gttggcgcat cccataaaat caagaagaag atcttttccg
    18601 aaattgaatt cattcgtcga ggcccacgaa gagggagctc ctcctgcaag attatttttc
    18661 cccgaccgtt cttcccagtt ggtgattgcc ggggtaaaat tgccgtaaac ctgtgtgaac
    18721 ccgaaatcat ttaggtcctt ctcctttttt tcactgtccc agaaccagtt aaagataaga
    18781 atatccttgg gtatcaattc tttcaccact tccggcctta ccgctcccgg tgtccggtat
    18841 ttaatgccgg ttgatgaagt ccagtccata aagagtgtat cgcgcacgct ttcgagaagg
    18901 taatcgcccc acattgccgt cctgattccc ttaccggtaa aatatccgtg tatctttttc
    18961 acatcttctg caaacaggac agaataatcc ttatccctgc ataccggaca aactcccaaa
    19021 ggcgctcccc accattcatc atgcccgatg tgcaccattt tcggtttcat cacttcgaca
    19081 tattcatcgt acacgtcaaa cataagttca tatgatgcag gatttgaagg gcagtaggta
    19141 tcgggccaga tatcacccgg atattccgcc agttccggat ggcgtgtaag aaggtaatac
    19201 gcatgggtaa gggatggaat ttcagggata acctcaatga agttacggtt tgaaagttcc
    19261 acaatattcc ttatatcggc tttttcaagt atatggccgt cgccggcgtc atgatgactg
    19321 gagttcttca cctcgccatg agggccctcg gtggaatttg accgggtata ttgcatgtat
    19381 tttgagaatt cgacccatcc ggcattgacc tcagggtgtc tgtcgagcct catgcagtta
    19441 acttccatta tcagtttgtt gaatttgtaa agcgccatga aatccctgat gaacctgtta
    19501 aagaatggga tattatcagg tcccggtaca tatagtctta ttcccctgaa tggcatttgc
    19561 ggccagtccc tcaccctgat gcccgtgacg gtgttcccgt ttccgttcct tatcagctgg
    19621 cgcaggctct gcagcccgta aaatgccccg gcatcgtccc agccggcaac tacgacaaga
    19681 cctgcatcca catgaagcag gtagccttcc ggcccgggat tgcgcgatgt taattcaagc
    19741 tttcctgact gacaatattt tttcaccagc ggattaccgg cggtacccat cagaattacc
    19801 ttcttatttt ccggcaaagt ggctgccctt tcagttttca ccgctatgct gtattttgaa
    19861 cttaattcac ccgaaagtga cctggccagg aacatgtcat tttctgaggc gtttaccggt
    19921 acaataataa ctgcttcctc actgagcggg aattcctctc cggtcagctg catttcccgg
    19981 ggaatcggaa aaactatggg ctttttaagt tcctgcccct ccaataataa agcagatgac
    20041 atcatagcaa aaaagacacc agccggtatc aatcctgaaa aactggataa aagcttcata
    20101 ttttcattgt tttgaattgt cagttttcat cattcattta attacctgtc ctgataccaa
    20161 tccgttaccg cgaaaggaaa cgttcaggtc cggttctgtt ttccaaaaga taaataacct
    20221 gccggagtta tctcctgagg ctcggaacag gttcagggcc gcgagaacaa attaataaaa
    20281 aaaagcggca gttcttacca tccctgaaaa tctttaaaat tatccctcaa ccaccgaaat
    20341 aaacatatcg tattctctaa ataattacat ttgttggttg aggtatccgg ccgggaatgt
    20401 tatatttgaa actgataatt tcaatatgaa caaatcaaag aatattctgc tgacattatt
    20461 ccttgctgct gtttgcattg cagtgattct gcttgcccgg acaaacagtt ccgggaggaa
    20521 tatcacaata ttgaatccgt tcaacgatgc actatttcct gctgaatttc ctgctcctgc
    20581 ctttgaatgg agaagccagc tgcaggatcc gtcgccctgg gaagtcacac tttctgtaag
    20641 gaagaatgat tattcgataa cggacattac ggataaaacc atctggagac ctgaagagcc
    20701 agcatgggac agtattaaaa aattgtcgga gaacgggaag atctttttcc gggtaaggaa
    20761 acacggaaac ggaggacggg gcaaacaggt tgttttcagg atcagtcctg attctgtcgg
    20821 ggccacggtt ctttacaggc agatgccgat accatttgta ctggccgaaa gggagctcga
    20881 atcgatgaat tatatgctga ttgatccggg cagtccctct ccgcctcaca ttgccatgaa
    20941 aggtttcccg gtctgtggga attgtcactc tttttcagat gatggtaaac tcctcggact
    21001 ggatctggat gcaggcagga gggataaagg aggttatttc cttactgcaa tcagtgatac
    21061 ggttgtattt gattccgaca atttcatgtc gtggtccaag catgaaaaaa ggagtacttt
    21121 cgggttgttc tcgaaaattt cacccgacgg aagatatgtg gtcacaacac tcaaggaccg
    21181 tgttgttgcc gaaaacccgc cgctcaactc agctgaaaac tttgcatact cccaattgtt
    21241 ctttccggtt aacggacatc tggcaattta tgacagggaa ttgaaaacac tgaacgatct
    21301 tcccggcgcc aatctggagg aatttgttca aagcaacgcc ttctggacgc ctgacggcaa
    21361 atcaattgta ttctcaaggg caaaggcatt gcccaggagt gacaatgtgt acgaaatcac
    21421 ggtttctgac agggaagtcc tcgattcatt cgtttcaggc aagaagccat tcaggtatga
    21481 catctgcatt attccgttca acaatggtaa gggcggtact gcagttcccg tgaaaggagc
    21541 ctcagggaac gggaaaagca attatttccc gacagtttca cccgacggaa aatggataat
    21601 atattgccag gccaatagtt tcatgaacct gatgcccgac agcaggcttt acatcgtgcc
    21661 tttaaaggga ggcactccgc gattgctcga ttgtaacatg tacgcgatga attcctggca
    21721 ttcgtggtcg ccgaacggga aatggataat attctcatca aaaggactga atatctatac
    21781 cgatctattc ctttctcata ttgataaaaa gggaaatgcc tccattcccg tgcttgttga
    21841 ccgggccagg gagtatcagc gagtctgcaa ttaccctgag ttcattaacc gcaatcctaa
    21901 aaacaagttc agcatgaaat atgattatgt tgaactggct catatcaggt atgccctgag
    21961 ggacaaagac cctgaaaaag caaggcagtt gttttacagg ctgatcaaac aggatccttt
    22021 catgttcagc gaggactatt cagagctcag cggttttctt taccgcatgg gcctgaagga
    22081 agaagcggtt aaatatgctg aaatagccgc caaatccctg aagtcaaata ttgaagccga
    22141 ctgatccttc atatgttacc aggcagatct tgcacggaaa ctgatcaaca caactggttt
    22201 tatcaatgtc ccggccatac ggtcagggcc ggtcaatgtt aaaaaaacct aatttctggg
    22261 tgcgatagat aatttaagta tatttgttca tcaacagttc tttcatgaaa atcgcccctg
    22321 cataacagca tgttatgcag gagaaacctg aaaactaacc aatatgaggc ttataaggat
    22381 catttcgttc ccagcagtgg ctgctgcata ttttgtatta acttcctgca gtgattcagg
    22441 gtctgacaaa tactacaaat cgtctgtaat agttcccgtc aacactttta ccatcggggt
    22501 cgagggtcct gcggttgaca ggcatggaaa tgtatacgcg gtaaattttg aaactgaggg
    22561 cacaatcggc aaaattactc ctgacaatca ggcgggcttg ttcatcaggc tacccgaggg
    22621 aagcattggc aacgggataa gatttgacag caggggcaat atgctgatcg ctgattatac
    22681 catgcataac attctaaaag tggatatggc cacgggtcag ataagcgtat ttgcaaatga
    22741 gccgctgatg agccagccca atgatatagc cattgacagc agggaccgta tttatgccag
    22801 tgatcctgac tggaaagccc ggaaaggaaa gttctggcgc atagataccg atggaagcac
    22861 aaccctcctc gacagtcttg gtacagcaaa cggaattgag gtaagccccg atgaaaaaac
    22921 cctgtatgta aatgcaggcc gcaccgtttg ggcctatgat ctttcttcag ggggtgatgt
    22981 aagcaataag agggttctta tcgaatttcc cgatttcgga atggacggga tgaggtgcga
    23041 cataaagggc aatctgtata tcgccaggtt cggcaaggga gttgtagcca tcgtttcacc
    23101 tgaaggcaat ctgctgcggg aagttcagct gaccgggaaa aggccgacaa atgttgcctt
    23161 tggcgggaaa gacgggaaaa cactgtatgt tactctgcag gatcagggaa acctggaatc
    23221 gttccgtgtg gaaacgccgg gaagggaatg gaaaatgcag aaaaatgtaa aaccaactaa
    23281 ataaaggatc taagatgaaa cgagtatctg ttcttattat catgttattc tttgttatgg
    23341 ccggcgcaat gaaagcccag acatacaata tcgtgataaa gggcggccat gtgatcgacc
    23401 ctaaaaacaa tatcaacgaa gtgatggatg ttgcggttaa agatggcaag attgcgaaac
    23461 tcgcaaaaaa catcgacgca aaggaaggca tccaggttgt caatgcccag ggcttgtacg
    23521 ttacacccgg gctcattgat attcatgtgc atgtttacca gggaaactat ctgaaccagc
    23581 agtacatgaa cggtcccaac tgcgttacac ccgacgggtt caccttcagg gtgggagtga
    23641 caacagttgt tgatgccggc tgcgcgggct ggagaacatt ccccgatttc aagcggcaga
    23701 ccatcgaccg gtcacagacc agggtccttg ccatgctcaa tatctgcgga gagggcatga
    23761 ggggtgatcc tttcgagcag aatctcaagg acatggatgc aaaactcaca ggtgagtttg
    23821 ctttgaaaaa ctcaaaggac attgtcggga tcaagcttgc ccacttcagc gggcccgact
    23881 ggactcccac cgaccgtgcg gttgaagccg gggaaattgc caacatgccc gtaatgatcg
    23941 atttcggagg cagcaatcct cccctttcca ttgaagagct gttttttaaa catctccgcc
    24001 cgggagatat cttcacccac tgttatgcct cacttggcaa cagcagggag tcggtggttg
    24061 atgaaaagac cggcaagctc aagccttttg tactcgatgc acagaaaagg ggtattgtgt
    24121 tcgacatagg gtatggcggt ataagctttg cctactcaca ggcgatccct gcaataaagg
    24181 gcggcctgct accgaatgct atcagcaccg acctgcatat aggcagcatg aatgccgcaa
    24241 tgaaggatat gctcacaacc atgtcgaaat gcctggcaat gggaatggat ctgcagagtg
    24301 tgatcagggc gagcaccagc gatcctgcaa ggatcatcaa aagggaagaa ctgggtaacc
    24361 tttccgaagg ttcagaggct gatatcgcga tcctaaacct tcgcaaggga aaattcggat
    24421 tatttgacta taccggttat aaaattgaag ccgacaggaa actcgaatgc gagatgacca
    24481 tcagggccgg caggatagtt tatgacctca acggcatagc tgatcccatt gttctggcaa
    24541 gaagaccgag ataagcatgt tattcggcgt gccggatcat taatttctgg caggaaatac
    24601 agaaccaatc atttaaaaca gtaaatttag atttacaaaa aagttcattt aaccataatt
    24661 aaaatcacta tgaaaacaga aagaaggaca gcattaaaga gaatgtttac atcattactt
    24721 ggtattaccg gggttgggct ggcagccagg gccggtacaa cggaaaagga agcaggcagc
    24781 atatccggtt ttcaacaggc gcccctgttt tcgggtcata caaaatatgg caatttggtt
    24841 ttcattgccg ggaaaggtgc gcatgttgct ccgtttgaca taaagaatca caccgaaatt
    24901 gttctcaaag agcttgaaaa agagcttatt aaagccggat catcaatgga gaaggttctc
    24961 aaggtgaatg tttatctgaa tgatattgcc gactacaaag gcatgaatga agtttatgcg
    25021 ggccgcttcg gcatgaatcc gccggtcagg accactgtgg ctgttgccaa gggcggtgtt
    25081 cccggagact cgcttgtgga gatggattgc attgcttaca tttaaaagat gcaagtattt
    25141 tgaaggaaaa ctaaatcaat aaatacgatt tttcatgaaa cgcagggacg caataaagaa
    25201 tctgtcggtg ttgcccgtgg ccggggcggt tgtaggtacc acactttccc ttgaatctgt
    25261 caccgcagcc ccggctgcac ctcctgcacc taagcggaac ctgttcaggg aactgggaat
    25321 aaggaccttc ctcaacgctg ccggtaccta tacggcaatg tcgggttgca ttatgcagga
    25381 cgaggtgatt gacaccattc gggcaaccgc aggtgaatat gcaatgatcg atgaggtgca
    25441 ggacaaggtt ggggaaagga tcgcagccat gtgccacgct gaagctgcca cggttacagc
    25501 cggatgctgg tcggctcttg ttctcggcac cgcaggtgtt ctgaccggga cggatatgga
    25561 aaaggttgca cggttgccgc acctggaagg aatgaaatcg gaggttcttg tacaaaagac
    25621 gcataatcac ggatatgtac atgctctcac aaacactggt gtaaggattg ttgaggttga
    25681 gactgccgat gatgttgaga aggccgcagg tcccgacaca gcaatgatgt ggtttctgaa
    25741 ttatgccggg cccgatggca aaatcagcga caaggaatgg gttgccctcg gaaagaaact
    25801 gaacatcccg acaatgatcg acattgctgc cgatgcaact cctgttgaga atctttggaa
    25861 attcaacgac atggggtttg accttgtgtg catctccggc ggaaagggcc tcaggggtcc
    25921 gcagagtgca ggaatactca tgggccggaa agatcttatc gccgcagcca ggctcaacgc
    25981 acctcccagg ggcgggaaca taggccgcgg aatgaaggta aacaaggaag aaatactggg
    26041 tatgtacgtt gctcttgaaa agttcatcaa cctggatcat gcaaaactct ggaaaacctg
    26101 ggaggaccgg attgccgtaa tggagaactc ggtcaaaaag atacccgggg tttcaaccag
    26161 cgtaattgtt cctcctgttg ccaaccgcac tcccacattg atcatttcct gggacaaagg
    26221 cagggtgaag gtgggccggg atgaattcat caaccggctg agagacggaa gtccgtctgt
    26281 tgccgttatg ggaggtaagg acgaaagcat aacaatcaca tcctggaacc tcttgcccgg
    26341 ccaggagaaa ctggtggcag caaggctgtc tgaggaactt accaaagcat ctgcctgatc
    26401 tttccggaat tacaaccaac agactctgaa tacttaaacc acggaggtgc accgggaaac
    26461 gaaggatata acaggtaata aacccgatat atttaactgt tctatccttt gtgaacacag
    26521 tcaagttccg tggtttttcc tttaaatacc aggctattgg catatactaa caacagatat
    26581 ccatgaaaca caaattatcc ttattcattc ttcttcaact gttgtcggcc ggttttgccg
    26641 ccgcacagac gtacagtatt gttattaagg gtggccatat tattgatccg aaaaacaaga
    26701 taaacgaagt catggacctg gccatcaaag acgggaaaat tgctgctgtt gcaaggaata
    26761 tcgatgcctc atcgggcaac agggtaatca atgcagccgg aatgtttgtc actcccggta
    26821 ttatcgatat gcatgtccat gtctattacg gcacaaagat ggaccaggga cttatgaacg
    26881 ggcccacttc agttcagccc gatgcctttt cattccgtgc aggggttaca actgttgtcg
    26941 atgtcggaag ttccggctgg cgctcttttc ctgactttaa aaggcagacc atcgatgctt
    27001 cccgtaccag ggttcttgct tttctcaata ttgcagcaga aggtatgcgc ggtggtcctt
    27061 ttgaacagac aaccctcgac atgaatccgg tgctcacagc cagatgcgcc agacagtatc
    27121 cgtccgacat agtcggaata aaactggctc actataatgg tcatgaatgg gccccgaccg
    27181 acagtgcagt tagggcgggc gagctggcaa aactcccggt gatgattgat ttcggttcgg
    27241 ccacaccgcc tctttccctt gaagaactgt tcatgaaaca tcttcgtccg ggcgatatat
    27301 ttacccatcc ctatgcttat ttccccgctt ccagggaaac cgtggtggat gaggacggca
    27361 aggttaagcc tttcgttttt gcagctgtga aaaggggaat aaaattcgat ctgggccatg
    27421 gcggaggatc tttcagatgg agccaggcaa taccttcggt gaggcagggc tttgtagcgg
    27481 atgcaataag ttccgacctt cataccggca gtatgaacag cggaatgaag gatatggcta
    27541 atcttatgtc gaagtttctg gcaatgggtc ttgatatcca ggaagtaatt ctccggtcga
    27601 cctggaatcc ggcaatgata atcaacaggc ccgatctcgg taatctcagc ataggatcag
    27661 aggcggatgt tgctgttttc agtttaagca gaggtgattt cggatttctc gacatcagtg
    27721 gtgaaaaact gaaaggagac aagaagtttg tagccgaact cactctcagg gccggcaata
    27781 ttgtctggga tctgaacggt atttccgcgc ctgataaaaa gtggtaagct aactttttaa
    27841 tacaaacaaa ttcgtctgat catgacagtc aaaaatcatt tcatttgcat tccgggttat
    27901 acaaggaatc tgttgtccag gttcatcatt ccggtaatta tggcaggtgc ttttgccttt
    27961 accactgctg aaggccaggc actcaacgat atccgtatag aacggcataa gcccgttcca
    28021 atgagcgacg gagttaagtt atatgccgac atttaccttc cttcagctcc gggcaggtat
    28081 cctgttctgg taacaaggac cccgtacggt gttcagaggg atggtgtcca tgaaatgatg
    28141 gtaaggtttg cacagcacgg gtacgcggtt gtggtaactg atgtaagggg gcggtatgag
    28201 agtgagggca aatgggagcc tttcagggat gaggcgaaag acggttatga aacaattgaa
    28261 tgggcagcca ggcagccttt ctcaaacggc aaggtggcca cacagggcgg cagttatgtg
    28321 gggcacaatc aatgggccgc tgccagcctg aatccgccgc atcttgtcgc agcattcccg
    28381 gcactggctt caaccaacat ctatgccaac tggctgacca tgggaggtgc tttcaggctg
    28441 agtttcaact atggctgggg agttgtaagg atgcccaaca ggataatgct tccccagtac
    28501 tggcacactg aaaaatacat gcctgaaaac ctgaaatacg acaatgtcct gatgcatctt
    28561 cccctgcaca acatggatgt tacgtttgaa ggggctccgg ttcagcatta ccgcgactgg
    28621 ctgacccatg aaagctatga caactactgg aaggaaataa gcgatgagga gcgttttgaa
    28681 aagataactg ttcccgttca tactctcgga ggatggtttg acatattcct gatgggaacg
    28741 ataaacggtt ttgtggggat gaagaaacac ggaggatcgc ctgaggccag ggctggtgca
    28801 aggatgatca tcggtccgtg gggacacgga ccaacgcgga ccttcggagg agtggatttc
    28861 acccctgatg ccttcatgga tatgtatgac ctgcagatca ggttttatga ctactatctg
    28921 aaagggataa acaccggact tgattttgag aagccggttc agcttttcta catgggggtc
    28981 aacagatgga ggagtgaaac cgactggccc ctacccggga cagaataccg tgagatgtat
    29041 atcagcagca acggctctgc taactcggtc cgcggtgatg gcagcctttc tttcaccaaa
    29101 ccggtaaaga aagcatctga cacctatcgt tatgatcctc ttaacccagt tcccacaacg
    29161 ggcgggaata actgctgcgg aacgcccact gccgcgggac cggtcgacca gaggcctgtt
    29221 gagcgaaggg aagatgtcct ggtttatacc agcgaatttc ttaaagatac cctgaccatt
    29281 gccggtccgg taaaaatgaa gctgtttgct gcaaccgacg gaaaagatac tgactggatg
    29341 atcaaactca ctgatgtcta tcctgacggc tattcaatgc caatttccga gggaattctc
    29401 cgtgccaggt ttcgcgaagg aattgacaaa atccggctcc ttaccccgaa tcaggtttat
    29461 gaatatgata ttgagcttac cggtacggca aatgcatttt taccgggtca tcgcatcaga
    29521 atagacatta cttcaagcaa cttcccgcag tttgacagga acccgaatac aggggacccg
    29581 ttgggaagca gtgcagtaac cagggttgct gtccagacga ttcatcatgg cggcacaaat
    29641 ctgagccatg ttcttctccc ggtggtaaaa ggagttaaat agtcaggggt tcaggtataa
    29701 atcaccattt cacgttcagc atcccagccc attcccctga catacatatt gtacatgccg
    29761 ggttgtgaga atccatccca caccacctgg tgaacgccgg gttccagatc gtcggcatgc
    29821 attctccata cgagctcgcc gttgccattc aggatatcga cataaacatc acctttctcg
    29881 ggaacaccga cttccattac aactttatct gccgcatcgg tgcccaatgc tcttattgaa
    29941 ggctgagccg cagcggcaat ttttcccgat ttccacgcct tgtagtcgaa tggttcatca
    30001 accatgtgat agcggggttc atcaataccg tcgctgctct ttctcagata atatgtcagc
    30061 agcggtgtac gcctgaatga atcaagcctg gcttttgttg ccttaccgtc cttccagaga
    30121 tagacaggaa tgtcaaaggg atccagtaca tccgacagtc ctctttctat gcctatatgg
    30181 aaaagcccga aattgccggg ttcatggccg gcgagccagt gtgcgataat atcgaccctg
    30241 aatgcatcct tgccaaatat gatgttgttg ctcatgaaat ccatgggctt gccattgtgc
    30301 gggcctccat ggaaaccgtc accgtcgcgg ccgtaaatac cctcaacaat atttattccg
    30361 gtaggggtca cgctgaatga atcgcacatt ctctgggccc attgctccat ccagaagcca
    30421 ccgttaaacg ggggtctgtc aattttctca ttccacctcg ggaatcctgc ctcaatatgt
    30481 tttttatgca gttctgctat ccggtccagg taatccggct gaaaaaatcg gtgatagcgt
    30541 ttgtcaaggg tcttgaacac atccacactt cctgcacaga acgaatgcaa tcttttggca
    30601 ttaattccct gcaggttctt tattgctcct gtaataccca tctgatgggc cttgaatttg
    30661 gccaggttga taagaaatgt atcttcagca gtcattggtg ccataaaggc gacttccctg
    30721 aatactatcc cgttgacggt cctgaatatt atatcatctc cgagttccca gtaatccctg
    30781 gcagtaaggt ccctcagatc gaaattattc ctttccgcca ttgcgatata tccattgggt
    30841 ttccactgga aggtgcatgc gcattccctc aaaaaaaagt tctgagggcc cttcgccttt
    30901 acccccctga ggtatccttc aatgaaattc aggtcggtgg ttatgccaag gcttgcttca
    30961 gggtcattct tatttcccgt gctgcatgtc cagtttggtt tgcaagtaat ccgggttgaa
    31021 ttcggtaaac ccttgcctcc tgatgttctg acaaacattt ctccggccag tttgtaggca
    31081 gcatcatgta ttgccgtggc atcggttttt tccttaaccg aggtaaggtt tatgaagacc
    31141 gcctcaggat gttccctgat aaacggatgc agatcgaatc cttcagccgg tgctcctgct
    31201 ggactgccgg ttaccctgat ccccggagtt acaaggccca gggcgcctgc tgcagaagaa
    31261 accttaatga aatcgcggcg tttcatcagg tatagataac catttcactc tcggcatccc
    31321 agcccatccc cttcacatat gtcgtgtaca aacccggtga tgaaaatccg tcccatacca
    31381 cctggtgaac ccccggttca aggtcatcgg catatagttt ccaaatgatc tctccgtcct
    31441 tgttcagaac gtccacgtaa acgttgcctt tttcaggaac agttacctcc attacgactt
    31501 tatcgtttga atcagtccca agggctttaa cggaaggtgc ttcagtcctg gcaatctttc
    31561 cgcctttcca tgcgttgtaa tcaaaaggct catcaaccat atggtacctg tcttcattct
    31621 cgccgtttgc gttccgccgc aggtacagag ttataagggg agtgcgtttc aggctttcaa
    31681 gcttaatctt ttttgccttt ccgttttccc acaggtacac tggtatatcg ttaggatcaa
    31741 gtacatctga aaatccccgt tcaattccga tatggaacag gccgaaattg ccgggctcat
    31801 gtcctgcgag ccagtgggtg atgatatcaa ccctgaaagg atctttgcca aagatgatat
    31861 tgttgctcat gatatccatg gctttccctc cctcatgagg tccgccggcg aatccgtcgc
    31921 catcgcgtcc gtatattcct tcaacaatat tgataccggt gggggttacg ctgtaggagt
    31981 caagcatacg ctgtacccat tgttccatcc acaggcctcc cccgtagggc gggcggtcca
    32041 ttgttgtgtc ccagcgcggt atgcccattt caacatgttt cttgtgcagt tcggcgaccc
    32101 ttgcaaggta atcgggctgg aagaactggt gataacggct gtcatacgat ttgaagatat
    32161 cataatggcc accgcagaac tgatggaact ttttcccggt tattccctgc aggttcttaa
    32221 ttgttccggt tatgcccatc tgatgcgtct tgaatttcgc aatattgatg agaaaagtat
    32281 cgggggcatt cattggggcc atatatccgg cctgcttaaa cacaaccccg tcgattttct
    32341 tgaaaattat ttcatcgccg agttcccaga aatccttggt tgacaggttc ttaaggtcaa
    32401 aattgttcct ttcagccatg ggtgtatagc cgttgaccac ccaggatgaa ggtgcggcac
    32461 attccctcag gtaaaattcc cggaggccct ttgcctttac cccgttcaga taaccttcga
    32521 taaaatcgat atctgtcgtg atgcccatat tggctgtcgg gtctttctta tcaatccggc
    32581 tggctgtcca gttgggcttg caggttatct ttgtcgagag gggatacccc ttcccttttt
    32641 ttgtttttac aaacatttcg gaagcgagct tgaatgctgc atcgtatttg gcttttgaat
    32701 ctgtcttttc cttaacggag gtaaggttta taaacactgc ctccggatgt gctttaataa
    32761 agggatgaag atcaaatccg ccatccgcct caaacggagc tgagaatgat tcaaatcccc
    32821 tgcctaccag gcctgcaact cctgcagcag aagatactct tataaagtct cgtcttttca
    32881 tatgaaaata aatttatata ctactgaaga ttaagtggtt cacatggaac ccacatgaat
    32941 gttattttca tgctttttta tgattttgta tcatgtgggt ttcatatcat tttttccttc
    33001 ttcatttatt tactgtcgcg taatcttcta tattaacgtt tgaaaaaata ttttgttccc
    33061 ttatctgatt aatgtcagca tattatcagg atgagagcgg ctgacccttg gggtagcgga
    33121 tttgccggcg cgtaccctta tagccgttgc gttttgctgt aaagttagcc ctgatatatt
    33181 cgccaaggta tatttccccc gaaatgacat cattggccag tttgccggaa aatgtaaagg
    33241 gcacattatc tgcaatcatt ctctccgtgc tgaacagttt aacctggtct ccgtcgattg
    33301 ttccggtcat ttcgcgcagt gagaagtcac ccttatgtga tccctgtatc cagtttccgt
    33361 cctgttcaag gataaacaca tgctgtgatt tgctgctgta gtattcgata tccacggccc
    33421 atctgccgct caggtcagct gccggggcct tcatcggggc cggtttcgga tattttttgt
    33481 tcagtatttc atatatgcgg tcagcaacag ttttatcatt cccgggctgc atctggcctg
    33541 cggaaataag gatcgaagtg cttccctcct ggtcgccaaa gccgctatgg acagcgatcc
    33601 tgggctttgt tgttgccagg atttccccaa cctcgggtcc ggttatatta aactgttccg
    33661 gatcccatga tatggcaagc atcgggctcc tgttgttcag gctatgcggt tcacgtattg
    33721 aggttttaat tccttttatc ccggtgactt tttttgagat attttccagg taggtatgcc
    33781 atgttctcat tttctcgtca tgatcgcgtg tcacccaatc ttcgactgca gcgagcatgc
    33841 ccatcatttc ttccttcccg accttattat cgcgtccggt tccatggtgg ggcgagcttg
    33901 cctgccaagc cgacatcagt atagccttgc ttccgagcag cagccctgca cactgcgggc
    33961 cgcacagggc tttgcccccg ctgtaggcca caacagttgc cccgcgctga agatgtacgt
    34021 tgggaactgt gagatcctcc gcagcagcat cgaccaggaa tggaatattg aggggttttg
    34081 ccagggcagc tattttgtct tccgcgaact cccgtccggg ttcggtatcg gcacccgtca
    34141 ttatatatat cattgcggtg cgcgggctga gggcttttcc cagttcctca agcgtattta
    34201 ccgtgatgat cctgacgcct atattgcgga ttgcatgatc ataggcgttt ctggagctct
    34261 tcgggataac cacctcggtt ttgtcaaagc cggtgagatc ggggatccga accattttct
    34321 caggatttcc cccggccaca caggcagctg taacgtgttt cattccggca gcacagcctg
    34381 cggagaccat tccccactct gccccggtta tttcagcaag gcgcctgcct attccgaaag
    34441 ccagttcatc atactggaca aaatgaccgg cagctgcatg agcagcttcg acaacagccg
    34501 ggcgctcaag ggaaccgccg attattgtat atgtgccgac acaattaatt accggatcaa
    34561 ctcctattga ttcatagata ttcggtcctg tcttcagcgg tccgctggca ggtccgacaa
    34621 ctgattccaa tggaagaaat gttccggcca gaggcagaac tgaaagattc ttaataaggt
    34681 ccctgcgtat catggtcata ggttttaaaa tggttcatca ttatttctgg ttaattttct
    34741 ggtgatcgag ccagttttcg gcggaaaggc cattctggtc ccaaacgatc cggccgtttc
    34801 gtattgtcat ttcagcctca agttttttag tgccctcaag cctgtgatta cgggtatcaa
    34861 taaatccgaa ctttccttcc ttcaggctga gaatgacaat gtctgcaact gctccttcgc
    34921 caagattacc aagatcttta cgatttaatg tgcccgccgg gccccaggta gcactatata
    34981 cggcatcctg aaggctcatt ccgatattga ggaatttcga aactatatcg agcatatttt
    35041 tcattcctga attaatgctc gacctgtgaa gatccgatcc gaacgaatcg ggcttcagtc
    35101 cctgcctgaa ggcgggaaca gcgatattga aatggaacat tccgcctccg tgtccaacgt
    35161 caaacctgac acctttcttt ttcgcctcca tcacaaatgg tttgattttc aggttttcat
    35221 caagaagaca gtcgcggtcg accgcggtgc agtagctatg agtgtatata tcgccgggcc
    35281 tgagccgccc gaggatctct tcaagcgggt actggggcat gtggcattcc acaatcagcg
    35341 gaacgtccga cagtttcgcg gcttcaagtg cccggtcgaa aggtgcccag tcgcttccgc
    35401 gatagtgtcc gatcttgaca cccacaataa tttcagggta ctgatctata accagcgacg
    35461 tcatccttgc atccatatcg ttaaggtcct cttcggaagg gaatcctgtc attcccgacc
    35521 cggcaatgtt gagaaatgca agtaccctgg tcactgacct gtcaataacc tgccgcttaa
    35581 aaacaggaaa attcctccag cccgaagtac cagcatccac cacggtggtt attcctgcct
    35641 taagggtaat atcatcggga gaaatgcaca tgaatccgtc ggcaaacccg ttattcgtgc
    35701 cgacaaaaac atgggtgtga atgtcaatca gaccgggcgt cacgtaaagt cccccggcat
    35761 caatgacttt ttttgcattt ccggcaggga tggaagcagc caccctgaga atttttccgt
    35821 tggcaaccgc aacatccatg atgccgtcaa tcttattccc gggatcaatt acatgaccgc
    35881 ctttgatcag aatgtcaatc tcctgggaat gcagaatcat aacctggaac agcaggatga
    35941 caaatacagg aatgattttt cccagacctg gtatgattac ttttttcagc attttttcac
    36001 ctttttttat gatcaccggg tttcccgtcc ctctgttttt tcctccgtgt ccctctgtgt
    36061 tctccgtggt ttatctgtgt ttctccgagc cctctgtggt tttcctccgt gtaccactgt
    36121 gttctccgtg gtttatctcc gtgcttctct gtgccctccg tggttttcct tcgtcctttt
    36181 ccgtgccctc tgtggttctc tccgtgtacc actgtgttct ctgtggttaa tctccatgct
    36241 tctccgtgcc ctctgtggtt ctctctgtgc acctctgtgt tttctgtggt taatctccat
    36301 gcttctccgt gccctccgtg gtttttctct ttgtacctcc gtggttaacg ttccctgatt
    36361 aacccctcgt tcagtttaaa accatcctcc tatctgccgc tgaccggttt gacttcatcc
    36421 cacatcgggg cagcaattcc gttcaggtcc cacacgaccc ttccgtcgcg gatggtcagt
    36481 tctgcttcaa gtttctgggt tcccttcatt ttcgtacccc tgacatcgag aaacccgaaa
    36541 tcgccttttc tgagtgtgaa aacagccaca tcggccggag cgccttcact caggtgcccc
    36601 agctcggttc ttttaataac ctttgccggg ttccaggttg ccctgagaat gacatcgttg
    36661 agtgaaagcc ccatattcag gaatttcgaa agcagattag ccatatcctt cattccgctg
    36721 ttcatgcttc ctatatgcag gtcggaagaa ataacatccg caatgaatcc ctgctgaaca
    36781 gatggtattg cctgtttcca gtgaaaggct cctcctccgt ggcccacgtc gaagattatt
    36841 cctctttttt gagcctcaaa aatgaaaggt ttgaccttgc cgttttcatc aaccggtact
    36901 tccctgtcct gcggcgcatt tgcataggta tgggtccaga tgtcaccggg acgcaggtgt
    36961 ttcataaaca gttcctcgat cggcagggga gggtgatgtt caccaaggtc caccatgacc
    37021 ggcacatttg ctattttccc ggcttccacg gccttgtcaa cctgtgtaaa atcgggtccc
    37081 cagtaatgcg ccgacttcac ccctacaagg atttccggga aaagccttgt tatcatatat
    37141 gaggtcataa ccggattcat gtctgtcaca tcctgctcct caaacctccc gtacatacct
    37201 gtgcccacga tattaaggaa tgcgagtatt cttgttctcg acctgtcaat agtctgcgac
    37261 ttgaaagccc ggaagtttct ccagccagcg gaaccggcat cgaccaccgt tgttacaccg
    37321 gccctgaagg taaatccgtc gggaggcagt gcatcccatc cgtcagcaat gtatgatccc
    37381 ggttctgttc cgtgaaatgc atgaacatgc atatctatta gcccgggtgt tacatacatt
    37441 cccgtaacat ccacaacccg ccttgcgttg cttgcgggaa tatcctttgc aaccctgagg
    37501 atcttgccat cggcaattgc cacatccatc cttgaattga tgttgttttt cggatcgatt
    37561 acatggccgc ctttgagaag gattgagatc tgctgcgaca gcaccggccc tgaagcaaaa
    37621 accatagcgg ccagcaggaa gatggcccta taagcatata tcctggtttt tccactcatt
    37681 tgataaaatt tattattttg aacattaaac acccgtcatg catacagggc aaagcccttt
    37741 attttaacac acggaagcct attcctgtgc aaataattaa caaattcagg tccataataa
    37801 ttcaaattta cacaaattaa tgccgaaatc catgccttcc tcttacctgg acctgaataa
    37861 aaaggcagtg tattacaaca ctacctgcca gttttacttt tcatttctga ccgacatcaa
    37921 tcctcttttc acctgaaata attccgttct gtctcagaat gcgaaagcgg agtaaaatta
    37981 caaattttcc ttagcccctg cagatcaggc gatgctttta ttcttaaaga ttgttaaatt
    38041 ggacaacccg gaaaaaactc atatttttgc atttcttaaa ttcatatttt taccctgaac
    38101 gtgtttgcga aattcacacg gaaaaagaag aaaaaaacaa ataaatcaga aaaatcatga
    38161 atcacaacaa attaaaatta aagagccttg ccgcagggag taaaacattt ctttccggcg
    38221 gcattttatt aataatcctg gcagcattgg ggttttcctc acctgcaggc cagcaggcag
    38281 caaaggagac tggcatctcc gccaaaaaat actcgacggt tgcagttgat gagaaaaaca
    38341 taaagtggtt tgtaactgaa aacggagttg tgagttttga cgggacaaaa tggaagcttc
    38401 acggttccaa tgacaatctt ccctctgatg tgaaccacct ggctctcgct ttttctgctt
    38461 ccgggccgga gttactggtt gcgtcttcaa acggggcaac tgttgcctca ctgcctcccg
    38521 atccccaaaa gggaaccagg gttttcaaag cgtctgattc gccatcaatg ggcgacaagg
    38581 tatcaagggt agccgcagga tcaaatctgc tgaaatggtt tgccacagac aagggtattg
    38641 cagcatacgg cgacggcaaa tggatgaaac ctgagtacac aagttattat cctgttgaat
    38701 tattcgagga atttccaatt acctcgctgg gcacgaacgc tgcaggcgat acactttatg
    38761 ttggtacata cggggccgga gttgcgagga ttttcaagaa caatgacctc gatgccattt
    38821 ctggggcatc tgaatatgcc atatgggggc cgataattat gccgtccgat accattcaaa
    38881 gtatttatat agcaagggat ggttcgaagt ggtttggtac aaacctgggg atcgcacaac
    38941 acaccggcag caacactctc gacaactgga ccgtttacac cactgaagaa ggccttgtaa
    39001 atgatgatgt tcaggccata acagctgacc tgaaaggcaa ttactggttt ggcaccaaag
    39061 gtgggatatc tgttttcaac ggctcttcgt ggaccaccta taccagaagc aacggcctca
    39121 acagtgacaa tatcctgtgc cttggcgtcg acaaggatgg agttgtctgg atcggaaccg
    39181 acaacggggt gaacagcttc agggacggac agtttacaag ttacaggtag ccggatttcc
    39241 gggcctgcgc cggccagggc agtagccgaa gatcatatac ggattctgga tcatcaaaaa
    39301 gatatacttc ccgtagtacc agatcaagcc ccggcggaag agaaagtaca aaagttgcca
    39361 actgttaatc gttaatctgc aactttttta gtcctgttcc ggatttacga cctgcccgat
    39421 gaacagcagg gtgtttgttg tacgctcgcg gatggcaaaa atgaatgact tgtaaatatt
    39481 gaacgggccg ggtgatgtaa ctcctacagc tattgttgtc actgctgcag cttccgttcc
    39541 ttcttcatca acttcaacga aggtgttctg tttcacatag tcgacccaga tctcactgtc
    39601 ggatatgcct gagagatctg cgtcggatga gctgaaagca tcaatcatac ccatgctcat
    39661 cagctggttg ttaaggtatt tctcatagga gaatttaaac ttcggcatta ttactgttat
    39721 tttgtcgaac tcatggagtt catcaaggtc ggagcaaagt ttatcccata cctggtaagt
    39781 aaatgaagga taaaaggcgc tcagggtttc attcggtact attaccacca tggtgaagtt
    39841 ggtctggccg taaggcattt caattgccct gaagccattg ccgcaggcaa acttcgcacc
    39901 caccttgcct ttcattgttg gtacatctgt tgcactgccg ccatcgggaa ggaagggaag
    39961 ctcctgtgtc atggatttgt caaactggca gctccagtct ccgttgaaat agagggcgtt
    40021 catgatgaac attacttcgt ctcctggtat ctcacttaac acttcaggaa tttttccttt
    40081 ggtattgtcg ctggcccatt tattgatcgt ggtgagggca gtaggagcgg caaagtcaag
    40141 tgctttgacg gttgccttgt aatcatcgct catggtggca aggaagggcg gttttacatc
    40201 gaatccatcg aggtagaaaa tcgcgtttgc aagggcgagc tgtaccctgg gatcaactgt
    40261 gagcaactgt ccgacaaggc ttttgtaagc ctcattgatt tcagagatgc tcatttctgc
    40321 cggatatttg agtgtcccct gtaactgcga ataggtatca ccgtcacaac cgttgagaag
    40381 catggtaagg gccgtgcttg cgctgagagg cgataacatg agattcttgt tttccgattc
    40441 agtcaccctg gtgaaaagct ctattccgaa ctcattgctg ctctgaatgg caatgggtgc
    40501 tgctgcagag agcgttatgt ttttcggtgt cagcttcggt tcttcattgt tttcctttga
    40561 acagcagacg gctaggagaa aaattgccaa tcctgacaaa atgatcataa tatttttctt
    40621 cattataata taattttgat tatttataaa ggtatgatgc ctgcctgtcg gcagacatgg
    40681 tatcacacat aattgaaaat tatataaata cgtgtttgtg catttgatat ttgtggggtt
    40741 catgatttcc actttttacg ttgataaatc attgcttttt attaactaat acgaaactaa
    40801 tgttatgtta atcgtaaatt gacggatata atttcttcaa tttcaccctt gcctgcttgt
    40861 ttgtgaactg ccagtttatt ttacatttct tgttattccg gttgctttcc caagccgcta
    40921 cttcctcttt tattttatcc atagcgggaa tatgccggtt caaacactgc ccgtttagta
    40981 catgcagctc tatctcagcc atattgagcc agcttccatg tttaggtgta tatacaaatt
    41041 caaacctgtc ccacaatctt tttgcttctt ctggtctgaa tgtttcatat aaagccgatg
    41101 ctccatgagt attataatta tccatcacta atgttatccg
//
LOCUS       DAOOYG010000010        40574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_817_length_40574_cov_7.354599, whole genome shotgun sequence.
ACCESSION   DAOOYG010000010 DAOOYG010000000
VERSION     DAOOYG010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 40574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_817_length_40574_cov_7.354599"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2057)
                     /locus_tag="PK288_01935"
     CDS             complement(<1..2057)
                     /locus_tag="PK288_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17704.1"
                     /translation="MKITASLFPGILRIALPVFILFVTNGCRNTGNNVREYGWFDFVI
                     PDTDSLSNDIDMSFLNESVAGASGFVTVKDGHFIDGKGERIRFFGTNLTFGSCFPDRE
                     TAVKIAARLRKLGMNVVRFHHMDNQSAPNGIWDKDRKNLDPGQLEKLDWLIYQLKIHG
                     IYTNINTHVSYTYPGMDYKDIEQFNYGKAIDNFYRPYIEMQKVYAEKLLKHVNIYTGN
                     AYSEEPAVAFVEINNENSLLSNWALLPRLNSAHMKNLITQWQLWLRSNPEYSKKEGYN
                     NDLIDIIANYGTKATSTAKEMMWAFLMNTEISYAKEMSEFLRNKLRVRALISESQASY
                     SGAAGILREAESSDYIDMHAYWEHPSFPGRSWSPTDWLIRNTSMVSDRKGGTLLSFDQ
                     HRVKGMPLTISEYDHPAPSFFCAEMFPMINSISAFQDFDGIYHFTFNQPFDEGRINNF
                     FSSAGHPLKQVFVPAGAVLFRMGAVQPGRQTISLNMPPGAVLGELVESGGELRLHGSN
                     MRSVWKKYGAHESLIALHRMDVNMEASNPGLSESVSEPAGPWESDTGEIIWDNNDSSN
                     AVFKVKTPSARVAAGYIGGKAVELGSITVEMDATPFNWATVTITSLDGKPLEVSSRIL
                     LSAAGRAENTDMKWNEGKTTVGSGWGEAPTKVEGIPARLVFRDMDRFRVHSLDPAGNR
                     VTEV"
     gene            complement(2387..3559)
                     /locus_tag="PK288_01940"
     CDS             complement(2387..3559)
                     /locus_tag="PK288_01940"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPM17705.1"
                     /translation="MKMKNTAIPKCNGLIKWSVFLAILILAGACKRSAISGEMTASVI
                     PPEPDPKGYVCYRTASPIKPDGILDEPSWNAAPWSDYFVDIEGDAKPAPAYKTRVKML
                     WDNSNLYIAAELEEPHVWATLRQRDTVIFIDNDFEVFIDPDGDTQNYYELEVNAFGTE
                     WDLLLTKPYRDDGGFPINGWDIKGLRTGTHVDGTINKPDDTDRGWSVEIVLPLAALRE
                     CAGIAGLPEPGDQWRINFSRVEWRAVIENGKYRKEINPETKRPFPEDNWVWSPQGRIN
                     MHMPEMWGYLQFSGLTAGAGTEDFIPDRDLGRKWALRMIYYAEKEYFKKYGKYTSCLK
                     DLGLKISDFPENVPTPRIERTRTTFECYFPKGNEESNLVIYNDGRLTDLKRSSENR"
     gene            3771..4943
                     /locus_tag="PK288_01945"
     CDS             3771..4943
                     /locus_tag="PK288_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HPM17706.1"
                     /translation="MEPSSYSRRKFLSAGSVLAASTVAASAAVPAGPVYFTAAPGMLN
                     AKDFGARGDGKADDTAAIQKALDTAGPVNGAVMIPEGNYLCSELKVPEGIGIYGMPAW
                     SFRRGMGTIITFNGKGRCLINLTGAFGAYLSGLCLNGKNIGENIHGILIDKPDYGTQE
                     DTPRIDTCRVERFTGDGIRLERIWCFCVRHSHLINNKGCGLRVRGWDGFILDNWFSGN
                     GKAGYGSYDENASNTMTGNRIEWNREGGIVIFGGSHYNITGNYIDRSGRYGLALLPGP
                     GGRNCEVITATGNVIYRSGKPEWGRQDDYDSSHVRLESVKGLVFTGNALHSGRDDGGK
                     GSFSPDYGIVLKNLESSIVKDNVLHNGALRELMADLGGHGEGVIIRDNVGSLRKLS"
     gene            4954..7569
                     /locus_tag="PK288_01950"
     CDS             4954..7569
                     /locus_tag="PK288_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPM17707.1"
                     /translation="MNRNILIHIMVFAFSLGTYAQSSTLLLHSNWKAKKAVDVLVDGT
                     MITGTDFTPVGWMDAVVPGTVLTTLLHNDLIPDPFFGMNNDLIPDVYDTGRDHYTYWF
                     YNEFEMPETGEDREVWLNFRGINYFADIYLNGNRVNTTVHAGMYLREKYRITPYLSRG
                     GKNRLAVLVAPPDPVGNAAAGQGGDGTIGRNVTMQFTAGWDWICPVRDRNTGIWDQVS
                     IETTGKVDLKDVFIETKVPGTRVPGAKQDPAFVKVSAELKNTSEVAINGILRVEFKGY
                     VNKVKVILPPSEEKNVTIGSIKVEDPELWWPNGMGDQPLYRMKIDFLQTDGTLLDSET
                     VTFGIRETSNTFDEKIKGQVFKVNGQKVFIKGGNWIASDALMRLSKERYEAEVRMHAG
                     MNMNMIRVWGGGLTERPEFYDACDKYGILVWQDVWISGDCNGEWDDPLKKESQARRKA
                     YPDDHDLFLRSVTDQVKMLRNHPSLYLWCGGNETYPPADILRALKEEIFPRCDPGRFF
                     LDMSTSPALMTNTIGGVGDGPYGIREPEKIFTERSFPFNPETGSIGIPNYDGLVKIIP
                     EDEMTPPGATGRPSKSWTYHKYLPLYDFPDRYGKVKDVRDFCLKAQIVSYEQYRALQE
                     AFNYKMWDWYTGMLVWKNQNPWTALRGFFYDYYLDYTGGYFGYMHGAAPVHIQLNLND
                     SVVCTLNQTARSIKDLEAEVLLSDLHGKQVFSRKSTLDLNAQETKLLDKVELPGEDSE
                     VFFLKLRLRKNNEIIDENLYWLTNKPKSYEKLNDLRNVTLKTDVSRTGQKNATVSVIN
                     QENETAFFIRLKIVDRQGNLVLPSYFSENYFTLLPGDSKQVNIELPAQPPTRKSDGIR
                     LVLEGWNTGPEEISF"
     gene            complement(7669..9231)
                     /locus_tag="PK288_01955"
     CDS             complement(7669..9231)
                     /locus_tag="PK288_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPM17708.1"
                     /translation="MKGRSLPLLQFAKGFNAMMLSCVLLLFAAGCSEDKEAPPVFKPI
                     DLNLFSQTHKGFNLLGKFDAGWSNTGFSEKEFEIIKDLGFNFARLPLDYRTYTKAGNW
                     NEFSEGCSEIDDAVEWGRKYGVHVCICLHRAPGYCVNKTILPANQDLDLWTDPVARAA
                     FVKHWEFFAERYRNVPWTDLSFNLVNEPGDVDESTYFSVMKMAIDRIRQINPDRIIFV
                     DGLNIGRDLVPSFRGMKNIIQAIHVYDPVTLTHYRADWIPGSDSWPLPVWPMTDISWY
                     LYGPVKPEYSSSLIFEGNFPGGTEITVNVHQVSVRSTLQIRLDENEVYKKVFICGPDP
                     GDDWTRIIETQWGYQNISGKDYTAVLPSGGTRLAFSNTDGDWLTVNKITIRFGATDSI
                     VIIPANTTWGARQNTYRITTERKITDTGGNPVVALSSLNNALKMAGEENIPVMVQEFG
                     VFNKTPHSVALAYLSDVVSILNRNKTGYAMWNLIGTMGIINSGRFDCSYEEYRGMLLD
                     REMTTVIQKPDR"
     gene            9475..10101
                     /locus_tag="PK288_01960"
     CDS             9475..10101
                     /locus_tag="PK288_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17709.1"
                     /translation="MKKKISFLVLSLLIIIASCSKTGEKLGGSPSPIGEEGVTFSTSS
                     GTIAGVSNVSASVTELKGGVSSISGSAVVTNPVIKNILSNHPEAQINGNNVTVTDVEF
                     RITTEGVESVYGLQPGIIVKYDAKVGDKYKVGDGITREVVARSTDNDYFWGGMLIKVI
                     QVEENTNKFGVKKITYWANHRFGLVGMEFQFDDNSTAKFPIFASAQNN"
     gene            10132..11403
                     /locus_tag="PK288_01965"
     CDS             10132..11403
                     /locus_tag="PK288_01965"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM17710.1"
                     /translation="MFKYLTINGERKRIPSYPHHKYFVWASYKYFSVRSLLGFLTLEG
                     PLFIPRRIYNNLKARCVVPLVLMIDPTSSCNLRCTGCWAADYQKNSNLSFEKLDETIG
                     EAVRLGVRQIFMTGGEPLMRKGEILELCRRHKGIPFGIFTNGTLIDENLATEAARAGN
                     LNFFISIEGFGEETDMRRGDGTFDKAVRAMDLLKKHDIAFGFSACYHSKNYRSVTSDE
                     FLDFVRSKGAWFGWMFNYFPIGDDADLTLCCKPEERAYAKEKIEAYCKKHNYNIIDFA
                     NSGHKSIGCVAAANDFAHINANGDLEPCAFLHYSDVNINDMPLTQALRSPFFKKFRKH
                     KPFSKNMLRPCPIMDVPEALTKITDLEGVHSTHMRCPETAARLAEKTIPLAEAWKPVA
                     EKMFEGMPAAEKRRFGILNWLLQSGNRMISG"
     gene            11440..11874
                     /locus_tag="PK288_01970"
     CDS             11440..11874
                     /locus_tag="PK288_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17711.1"
                     /translation="MLSFKWKIAGYTIAVLSAILTVLYFAAGFRFEIPVFAIVSSYRE
                     IKFLSTFSTNFADETILLGFISGFALIAFSKERKELDIYREIRVNALIKAVVIYTLFL
                     IISVLFLYGSVFMGIAILNLVLPFVLYILIFNILKFRAQREP"
     gene            complement(11969..12730)
                     /locus_tag="PK288_01975"
     CDS             complement(11969..12730)
                     /locus_tag="PK288_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HPM17712.1"
                     /translation="MLAKQWLENARAVMTRIEETQMDNIRKAAELMADTIECKRWVHT
                     FGCGHATLPIEEMYPRIGGFVGFHPMIELPLTFFTRITGEMGVHQFVFLERVEGYGNE
                     IMKGYDFDKRDIMWLFSHSGINNVNIDIALESRKRGMKVIVFGSAEAAKGKKTRHSCG
                     KTIFELADVVVDTCAPLEDASVILKDHQDKIGPVSTMAFVTCVWMTVTTVAEILAGRG
                     VKLYIHPSHNVPGDGTAKQRLDEALAEYKRRIAGV"
     gene            complement(12849..14012)
                     /locus_tag="PK288_01980"
     CDS             complement(12849..14012)
                     /locus_tag="PK288_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM17713.1"
                     /translation="MYNKKVVFRSACLGIFLFGIALISLGSLAPDLREKFRLDEIASG
                     TLFSILPFGILAGSLVFGPIADRYGYRVLLALSCILLAAGFEGIAFSQGTGLLKVCIF
                     LVGFSGGSINGATNALVSDISDRDKGANLSLLGVFFGIGALGMPMLLGILEKSFSSGQ
                     IIAFIGLLSFATGILFLLIKMPQPKQMQGFPLGKSLRFLNDRFIILVAFFLFFQSSFE
                     GIINNWTTTYLIDQAHIPQGSALFTLSMFVAGMAIMRLVTGTALRNVPVGRILVISFA
                     MIIAGLIIIRTGEILPFLMTGFFLLGAGLAGGFPVMLGFVGEAYADLSGTAFSFVLFI
                     ALLGNMLINYAMGIISKNFGISHLITVAFCETIIMIALLFCILKLKKDKSMPA"
     gene            complement(14029..14985)
                     /locus_tag="PK288_01985"
     CDS             complement(14029..14985)
                     /locus_tag="PK288_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPM17714.1"
                     /translation="MNVLGIDLGGTKLACAIFSENGDLLVKETVAIGKRKGPEVGSLI
                     TGTIVDLIDRSLSQGTDIRSIGISVPGIFSSKTGRVWAPNIEEWTDYPLLSEVSSVSG
                     GIPVAVDSDRACYILGEVWKGNARGCRDAVFLSVGTGIGAGIMVNGEILRGSNDIAGS
                     TGWMALNRPFREEYAACGCFEYHASGSGIAMVAREILSGHKEYDSILGKAAGDEITSR
                     EVFEAYDKGDKVAVMVIKEAVEYWGMAVANFVSLFDPEKIIFGGGVFGPAVSLLPDIA
                     EAAARWAQPVSFARVSIDVSGLGGDAGVYGAGRLALRNAENQ"
     gene            complement(15010..15864)
                     /locus_tag="PK288_01990"
     CDS             complement(15010..15864)
                     /locus_tag="PK288_01990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM17715.1"
                     /translation="MANYPRIFLNLLFLLVLAVSCRNKGNTSDNTNNSKSETSVKTAK
                     QQVRPGFVYESGAVIRGDTTADMIALVFTGDSFADGGDFIAETLREKNIKGSFFFTGN
                     FYRNKMFHPLIKRLVSDGNYLGSHSDRHLLYCDWEKRDSLLVTREEFVRDLEDSYAEM
                     KKFNIDQAGSRYFMPPYEWYNDTIASWTNRMGLVLINFSPGTRSNADYTYPEMGDRYL
                     DSRCILKSVLDYEKFSSNGLKGFILLVHIGTDPRRTDKFYSFLPDLIGTLINRGYVFV
                     RIDELLKN"
     gene            complement(15876..16985)
                     /gene="nagA"
                     /locus_tag="PK288_01995"
     CDS             complement(15876..16985)
                     /gene="nagA"
                     /locus_tag="PK288_01995"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPM17716.1"
                     /translation="MTTAFLNAVTITGEETIEGAALLIEGDRIIAIKEPSHLPPDINI
                     IDCHGHYIAPGLIDLQIAGSGGFLFSETPTAEALRTIAGSIVMTGTTGFLIAIPTNTF
                     AVYNEAAAVIRDNPLPSVLGLHLEGPYLNIKRRGAHFLSCIKKPDRDEIDQLMKDLGG
                     VVRMMTVAPELCDREIIECLNGHNVVVAAGHSNATYNEATRGFMWGIRTTTHLFNAMS
                     QLHHRDPGLPGATFMNGNVMASIIADGIHVDYTMVSIAKKIMNDRLFLVSDAVEESSQ
                     PTNPHIRRKDRFTLPDGTLSGSALTMLTAVRNCVEKTGINLDEAIRMASLYPARLMNL
                     HDRGKIKPGMRADLVSFDKDFNVSTVVLNGEILTN"
     gene            complement(17007..19793)
                     /locus_tag="PK288_02000"
     CDS             complement(17007..19793)
                     /locus_tag="PK288_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPM17717.1"
                     /translation="MKRNVNYLICLMLFFSYQAYSQPGKVKQVKLERFELQSSAVITE
                     TGDKLSVPEYRSVVYWFPVRVPSTVLTGLVANGIYPDPYNGMNNMLIPDASDEFNKRY
                     NLEQYSYLPNEPNPWKKPYWYRTTFDVPATDKGRCFQLIFKGINYRAAVWVNGLQIAD
                     STEMAGMFAQYSLDVSKQIRAGGKNALAVKIYPLDYPGLPDTEQLEALGDFYLNGGPT
                     GDIGKNVTMACSVGWDWVPPVRDRNMGIWLPVYLRTSGNVTIGDPRIDIRFADGQDTS
                     AAHISLSMTLFNHGNNNERGDLTVKISPENFTGRSVQFSKSITVSGGGRNQVELDPGN
                     TRELLIENPALWWPNGYGKPNLYRIRLQFSNESGISDDTTFLFGIRTVSSKPVDVKGS
                     LRRDFYVNGRRIHVTGGAWVPDFMVNRDSLRYDYELRLCRNANINLVRIWGGGVTPRD
                     EFFEAADRYGLLVWSDFWITGDTQGEFKGSPDWPLESDVFKRNVESTILRIRNHPSLL
                     VWTGGNEGHARKELYDFMRNSVIRLDGTRPFIPSSSGFAKIPDGWEGSWPDNQAGGVY
                     SGGPYTWQDPAEYYKKAIEAKDWVFKDETGIPSMPPYDILHKIIPDLVWDRTLPFPLN
                     HTWGYHDAATGNGRWDLYYHEMIRRYGKPVSMEDFCNKMQLMNAIGYQGIFEAAGHKL
                     NDIGGVMLWKLNAAFPSVVWQVYDWYLQTNAGYYFMQNACEPVHIQLNLANNRVTVLN
                     RTYKPAPGLVSCVDLYNMKSELLFHEEKKVSLGPTDVKETSSVGQRLAKMKELVFIVL
                     SLKDASGKVISSNIYWISPDKNYSPMSEMQETPVNVRITDERKTGSDISWTVEVSNPG
                     SRLAFFIRSQIVTGGEEVLPSLWSRNYFSLAPGESTVVNVTCPQAGLKDNNPVLRISG
                     WNVKTVETPAAKGY"
     gene            complement(19854..20345)
                     /locus_tag="PK288_02005"
     CDS             complement(19854..20345)
                     /locus_tag="PK288_02005"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPM17718.1"
                     /translation="MRIKISFLIITLLLSGSCAGTKKNRQISDSRNTENYIPGTTARK
                     TLMPSAPVEEKKPVAPAEGEREVQGIEKKIVPGDDNKINREKYFVIVGSFREPANARN
                     RQSEAKREGFRPEILKTEEGLYRVSVLATDDENEARSEVRRIWLMFPQYSDAWMLINK
                     TLE"
     gene            complement(20414..21787)
                     /locus_tag="PK288_02010"
     CDS             complement(20414..21787)
                     /locus_tag="PK288_02010"
                     /inference="COORDINATES: protein motif:HMM:NF018583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HPM17719.1"
                     /translation="MVTVIIPALNEEKTIGHVIGLVSASSLVDEILVIDDKSSDNTIK
                     KARLPKVKIYTSPKLGKGGSMRDGLLLSKNEINVFLDADILSYPDNVIELLTAPLLND
                     EADFVKSSFSRQAGRVTELVAKPLLSLLFPDLVKFSQPLSGMIACRKSWMEKIIIEND
                     YGADIGILLDMHLLGARIMEVNIGYIENRMQTWEQLGKMSREVSKAILKRVHNVDEKN
                     LETLENISIIRSQMDYAIRESLIGLEKMIIFDMDNTLLEGSFITKAAEEFNFENELIR
                     IVTEYSNAYTRTKSIARLLKNRTFAEILDVVDKIKIIDDAEYVIGELKKKGYICGIIS
                     DSYDIVTNHLKNKLRMDFSVANELEFSQSIATGEVKVPSAFMRNRHSKCNHDFCKSNV
                     LFQLAEKYNIDTKNIISVGDSENDICLVRESGIGIAFRSNNNYLNLVADRVINEKSFL
                     QILDIAY"
     gene            complement(21831..24446)
                     /gene="mutS"
                     /locus_tag="PK288_02015"
     CDS             complement(21831..24446)
                     /gene="mutS"
                     /locus_tag="PK288_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPM17720.1"
                     /translation="MFNPEEQKYAETPLMKQYYNVKSKHPDAILLFRVGDFYETFGED
                     AVKASEILGITLTRRANGSASFVELAGFPYHALDTYLPRLVRAGQRVAICEQLEDPKL
                     AKKIVKRGITELVTPGVSLNENVLSHKENNFLAAVHIDRKAAGVAFLDISTGEFLASE
                     GTMEYIDQMLNNFQPKEVLYEKRKGDLFQEAFGNRFYTYKLDDWIFTSEAANDRLLKQ
                     FETSSLKGFGIQNLTYGIIAAGAILHYIDITQHARLGHITGISRIEEGRYVWLDKFTI
                     RNLELFHSPYEGASTLIDIVDRTISPMGGRLLKRWISLPLKDIQPINDRLDIVAFFVA
                     NPSMMKEVSELVRQIGDLERLISKVAVGKINPREVVQLKRALRAIEPLKELLITSNCP
                     ALATLGDQLNPCRSVADRIDRELTADPPVQILKGQVIASGVSEELDDLRRILYSGKDF
                     LADFQQRESERTGITSLKVSYNNVFGYYIEVRNTHRDKVPPDWIRKQTLVSAERYITE
                     ELKEYENKILGAEEKILGLETRLFNDLVLAILEYIPPVQVNAGLIARIDCLYSFACVS
                     VNNNYVRPVIDDSKRLDIRDGRHPVIERQLPPEEPYIPNDLSLDTDNQQIIILTGPNM
                     SGKSALLRQTALIVLMAQTGCFVPAREANIGIVDKIFTRVGASDNISLGESTFMVEMN
                     ETASILNNLSDRSLVLLDEIGRGTSTYDGISIAWAMVEYLHESRFRAKTLFATHYHEL
                     NEMENSFMRVRNYNVSIKEFNNKVIFLRKLRRGGSEHSFGIHVARMAGMPKSVVTRAG
                     EILKELEQSHEKQELTKPITQLADHREGMQLSFFQLDDPVLKQIRDEILDLDLDNLTP
                     VEALNRLYNIKKYLK"
     gene            complement(24587..25297)
                     /locus_tag="PK288_02020"
     CDS             complement(24587..25297)
                     /locus_tag="PK288_02020"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPM17721.1"
                     /translation="MSNNYFTFRQFTINQERSAFKVGTDGVLLGAFAEVNGRRKILDV
                     GTGTGLIALILAQRSDAEIVAIEPDRGSFTDAAENVLHSRWAGRIQVHNCRLQDFEPE
                     GEKFDLIVTNPPYFIDSLRNPDPVRAITRHNDSLSHRDLLEGADRLLKEEGLLQLILP
                     YPEGNVFIAEAQTHGFYCNHIVKIRPVPTAPIRRLILTLSRKTGKAREQFLTIETGRR
                     HHYTDEYVSLTRDFYLKF"
     gene            25800..27143
                     /locus_tag="PK288_02025"
     CDS             25800..27143
                     /locus_tag="PK288_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM17722.1"
                     /translation="MSNKLSGNPITTIPETGNRPAFRNYRWIIVVLLFFATTINYIDR
                     QIIGLLKPLLDVEFSWTETDFARIVMAFTAAYAIGLLIIGRFIDKTGTKAGYSVIISL
                     WSIAGMLHAVARSVFQFGLARFALGLGESGNFPAGVKSIAEWFPRKERGLATGIFNSG
                     TAVGVVLALVIVPWILRHFNWHGVFWITGALGFVWLIFWLIYYDHPAKQKRLSSGELK
                     HIIEGQANEEQPKAGLKWHRLFAFPQTWALITGKGLIDPIFWFFLFWLPSYFSSAYEL
                     DLKRPSLELIIIYTATTVGSILGGYISSVLVKKGWPVLKARKASLLAFAVMELSILLA
                     QYTTAAWMAVAIISLAVAVHQAWATNVFTMASDMFPSEAVSSVVGIAGMAGAVGGILF
                     PILVGYLLDTYKAAGNLTGGYNLLFTICGLTYLTAWTVMHLLTRKHASVSLGELR"
     gene            27308..30595
                     /locus_tag="PK288_02030"
     CDS             27308..30595
                     /locus_tag="PK288_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPM17723.1"
                     /translation="MKTTIQKIPLILVTLMFTLRVSAAGEARLLRFPDINNNLIAFVY
                     AGDIWTVDSRGGEARRLTSHAGMELFPKISPDGKWIAFSGEYSGTRQVYVIPSEGGSP
                     RQLTWYNPVGVMPPRGGWDNVVLDWTPDSRQILIRANRTPFGDRNGKYFLVSLDGGLE
                     KPLQIVNGGFGVFSPDASKIVFTPVDREFRNWKRYKGGRASDLWIYDLKTDKSEQITD
                     FEGTDQLPTWYGDNIFFASDRDLKLNIWKYNTLTRQTTQVTKHTEFDVMWPSGNGDQL
                     VYENGGFIYRLNLNTGQTEKVSVSISYDNPNLVPYYRNVQENIHSFAVSPTGKRVLFD
                     ARGDIFSVPAEKGTTENLTMTQGIREVYPAWSPDGKYISYYSDQTGEYELYLLENKKE
                     ARPRQLTFNSSAWKYQPVWSPDSRYLLFSDRTLKLKLIEAASGKITDIDHATASELRN
                     YNFSPDSRWITYQKEGPNDKPAVWVYEIPTGRRQQVTSGIFSDDSPVFSLCGNYIFFA
                     SNRDFNLSFSDYDFDYIYNKATRLYAIPLTGKSPRIFRDKNDTEPVKETEKAGTSSPK
                     PKTAKGKSAAAGADTTAGKEIRVDIDFDGINERITLLPGEAGDYRIVGASEDGLVFIN
                     DGRLMKFNLKEEKNEEILDKVMTASLTADGKMAVYRSGKDFGIIRLTPGQKAGAGKIN
                     LDGLEMKIDPRKEWDQIYADAWRIYRDYFYVDNLHNVDWPAIKERYSVLLPYVGHRAD
                     LDYILNEMVAESNTGHSYVDWGDFEKVKRIENGLLGAELTADESAGRYRISSIYSGEN
                     WNPARRSPLTEQGVDVREGDYLIAIDGREITTSDNPYAFLENKAGKTIEIMVNSKPSP
                     AGARTSSVKPVTSELELLYFNWVNERRAMVDRLSGGRIGYIHVPNTSFEGNRELFHGI
                     YTYFDKEALIIDERYNGGGFIPDRMADLLDRHTLVYWHQNGLTPGKSPAVAHDGPKVM
                     LINGYSSSGGDAFPYFFKKLGLGKLIGTRTWGGLVGISGNARFVDGGYLSVPRFGIFD
                     SDKGWIIEGIGVNPDIEVMDRPEQLAKGIDPCIEKAVEVLLQELKDRPAKKVVAPAPP
                     DRSKWHE"
     gene            30685..31443
                     /locus_tag="PK288_02035"
     CDS             30685..31443
                     /locus_tag="PK288_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPM17724.1"
                     /translation="MIIQYASDLHLEFPENREFLKKRSLKPRGDVLILAGDIVPFRLM
                     YDHSDFLDYLSAHFDITYWVPGNHEYYHSDISKRSGSVNEKIRDNVFIVNNTTVNRGK
                     VCFLFSTLWTKIGPGHQVELRNRYSDFRVIKSGGRPYSINHYNLMHEKCLSFLSDELK
                     IQRRGPVIVVSHHVPTFSNYPEEYYGDPMNEAFAVELSDLILSGQPAYWIYGHLHSNK
                     GEFNIGKTILATNQLGYVSYHEQEGFDPGKIIVI"
     gene            31467..32069
                     /locus_tag="PK288_02040"
     CDS             31467..32069
                     /locus_tag="PK288_02040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM17725.1"
                     /translation="MNFPLKAIRYRTFIDPLLAGLRNEVLLKINSRDRVLEIACGTGM
                     LAMAMASKASHVTATDLSEENIAAASQSAGKNGIENLDFGVLDASDLSCYSGREFDAA
                     VISMAIHQFDQELAVKILLELKNIASRIIIADYSHHMPRGWGRKLATAIEWLAGGNHY
                     RNFRNYMAAGGIPYFAAKAGIKPVSRSVRGRGIFLVTVCN"
     assembly_gap    32425..32524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            33476..34729
                     /locus_tag="PK288_02045"
     CDS             33476..34729
                     /locus_tag="PK288_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPM17726.1"
                     /translation="MDDYTDMVVMLGTDTVPYEGSFIMDGNKTLTVTTKQMVLWKYQT
                     EKAVYYSTPAIGDDGTLYFGTGMGGTSDGAFFAISGDGTLKWSFAGQYSFFSPVIDAS
                     GHIYVQDFGYNCYAFYPDGKLKWKLPNTLFPRMVENVGQRCPAISADGTIYICADGLY
                     AVSPTTREILWHFPESRTKSSPAIGPDKTIYVVFRQDRVKAINPDGSLKWENRFDNPW
                     EMSYAAPAVDDRGFVYIASEAFYEGKDYSALYAFRQDNGEIQWRYPVGLFVRASPVIG
                     TNRSVYIATKATETDIARLICLSPEGEKRWEFEVENVHGMPDDIYSTPTLGADGLIYF
                     GAETGFLYAVRASDGVLAWKFDLKMTVNWSSPVILKNGTLYIGGMGGTDYEGYMKAIK
                     TGCPGYAPSPWPRFRHDNKNSGASD"
     gene            complement(35244..35927)
                     /locus_tag="PK288_02050"
     CDS             complement(35244..35927)
                     /locus_tag="PK288_02050"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HPM17727.1"
                     /translation="MKKLLFPLFSILSLYTHSQDIKIKGLISPLCIFDDYSFPTIQHG
                     FELRLTKRLALYSEAGIKFMKTSYEYSDTSFVKSKGYKLKTEIRYYVKNWNNQKGTNE
                     RGLFLGINTFCIRHSFNDLLYYTKDSDPDIGIPDSFSVLKHVWGANLVFGWQQVFIKH
                     IVIESYGGFGLRRRKTENHNLQFNPSMDEIVHSIDPNYEVIMYSINSEAGIRSLFNFT
                     AGFRMGYQF"
     gene            complement(36171..36392)
                     /locus_tag="PK288_02055"
     CDS             complement(36171..36392)
                     /locus_tag="PK288_02055"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HPM17728.1"
                     /translation="MIQTGKILVIAGVVLVIAGLIIWFLGDKLNWLGNLPGDIRIERE
                     NIRFYFPVTTMIVVSVILSLLLWLIRKLF"
     gene            36659..38560
                     /locus_tag="PK288_02060"
     CDS             36659..38560
                     /locus_tag="PK288_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019968694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPM17729.1"
                     /translation="MKNLITSLLAFALMAGLNAQNTDSIWIVNNYTKIERMIPMRDGV
                     KLFTSIYIPRDISEDHPVLMERSPYSCAPYGEGAWRDFWNRYLKYYLFEGYIMVKQDV
                     RGRWMSEGEFADIRPFNPHRKDNSDIDESSDTYDTIEWLLKNIPHNNGKVGVFGISYP
                     GFYSTMAAASNHPALKAVSPQAPVTNWFIGDDFHHNGAFFLLDAFRFYPALGAGFGLP
                     RPKPSTKGPENIRLPYQDNYRFFLETGALPAFARLAGDSVPFWKELYSHPDYDEWWKV
                     RDARNATKNLNPAMLWVGGHFDAEDNWGAWNSYKAAEENNPMKEFNRIVMGPWAHGQW
                     SWPDGSYLGNIGFGSNTSQWYQQNIEIPFFNYYLKGKGSKPEIAEATIFITGRNEWKE
                     FGSWPPAGTVNTDLFLQPSGRLGWQRPGSGPEHSDYVSDPSKPVPYTEDVHFLRTMEY
                     MTDDQRFASRRPDVLVFKTDILEEEITLTGMLTADLFVSLSSTDADFVVKLIDVFPDD
                     PGHLRTEPYSVRMPFSKYPGGGYEMLVHGEIFRGRYRNSFEKPAPFVPGKVERVKFEV
                     GNVAHTFKKGHRIMIQIQSSWFPLADRNPQKFVNIYEARESDFQKATIQIYHSKKYPG
                     KIILPVLTQ"
     gene            38652..39068
                     /locus_tag="PK288_02065"
     CDS             38652..39068
                     /locus_tag="PK288_02065"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HPM17730.1"
                     /translation="MLIYRIIDIFFVLFHTCLIIFNLSGWLWKRTRKLNLVFLLLTGG
                     SWLFLGMLTGTLGYCPLTDWHFRVLEKLGYTNLPASYIKYLCDRLTGIDFNAILIGRL
                     TFYLFLVALILSLLLNLRDFRKSPPEKLAASGKDNC"
     gene            complement(39123..40085)
                     /locus_tag="PK288_02070"
     CDS             complement(39123..40085)
                     /locus_tag="PK288_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM17731.1"
                     /translation="MTNEIKIHDLNAENFITERVNEIVSAVGQGTAINALSGGVDSSV
                     VTLLGHRALGHRLRTVFVQNGLMREGEAEFVADIFSKLGVKVEIVDARKEFLDALKGI
                     TDPEEKREAITQTFYKKVFGRIVRESGAKFLLQGTILTDIDETVAGIKRQHNVFAQLG
                     IDPEETFGYQILEPLIRLRKDGVRVAGKALGLPEEMFNRIPFPGPALAARIIGEVTAE
                     KLDLVKKATSVVERILAGTGAFQYMAILHNDRVTGMREGKRKFGHQIEIRCWDSVDAR
                     TASPTKLPFETLEELASSILENVPEVVSVTYNIASKPPSTLEAI"
BASE COUNT        10764 a         9600 c         9284 g        10826 t
ORIGIN      
        1 acttccgtaa ccctgttacc cgcaggatcg agcgaatgta ccctgaacct atccatatcc
       61 ctgaatacca gcctggcagg gatgccttcc acttttgtgg gagcttcacc ccatccgctg
      121 ccaacagtgg ttttcccttc attccacttc atatctgtgt tttcagccct ccctgctgct
      181 gaaagcaata tccttgaaga aacttccagc ggttttccgt caagtgaagt aattgtaaca
      241 gttgcccagt tgaaaggggt ggcatccatt tcaacagtaa tgctgccgag ttcaacggct
      301 ttgcctccga tgtagccggc ggcaaccctg gctgaaggtg ttttaacctt aaacactgca
      361 tttgatgaat cattattgtc ccatattatt tctccggtat cgctttccca gggacctgca
      421 ggttctgaaa cagactcaga aagaccgggg tttgaagctt ccatatttac gtccatgcga
      481 tgaagtgcta taagcgattc atgcgccccg tattttttcc agacacttct catatttgat
      541 ccgtgaagcc tgagttcacc gcctgattcc accagttcac ccaatacggc acccgggggc
      601 atattcagac tgatggtctg cctgccgggt tgtacggctc ccatcctgaa gagaactgca
      661 cctgcaggca cgaatacctg tttaagcgga tgtccggcac tggaaaagaa attattgatc
      721 ctcccctcgt caaatggttg attaaatgtg aaatgataaa tcccgtcaaa atcctggaag
      781 gcagatattg aattaatcat cgggaacatc tcggcacaga agaaactggg agccggatga
      841 tcgtactcac ttatagtcag gggcattcct ttcaccctgt gctgatcgaa ggaaagcagg
      901 gttcctcctt tcctgtcaga aaccattgat gtgttcctga tcagccagtc tgttggcgac
      961 cagcttctgc ccgggaagga aggatgctcc cagtatgcat gcatatcaat ataatcagat
     1021 gactccgcct ccctcaatat accagcagca ccactgtatg atgcctggga ttcagaaata
     1081 agggccctga ccctgagttt attcctcagg aactccgaca tttccttcgc ataggaaatt
     1141 tcggtattca tcaggaaggc ccacatcatc tcctttgctg ttgatgttgc ctttgtaccg
     1201 taattggcaa taatatcaat cagatcgtta ttgtatccct ccttttttga atattcagga
     1261 ttggatctca gccataactg ccattgggtg ataaggtttt tcatatgggc actgttcagc
     1321 cgcggaagca atgcccagtt ggagaggaga gagttttcgt tgtttatttc cacaaacgca
     1381 acagcaggct cctcggagta ggcattaccg gtataaatat ttacatgttt caataacttt
     1441 tcggcatata ctttttgcat ttcaatgtat ggcctgtaaa aattatcaat cgcttttcca
     1501 taattgaatt gctcaatatc cttataatcc atgcccggat aagtgtagga tacatgagta
     1561 ttgatattgg tgtagatgcc gtggatctta agctgataga taagccagtc aagtttttca
     1621 agctgccccg gatccaggtt tttcctgtcc ttgtcccata ttccgtttgg tgctgactgg
     1681 ttatccatat gatgaaacct gacaacattc attcccagct ttctgagcct agccgcaatt
     1741 tttaccgctg tctcccggtc gggaaagcat gatccgaaag tcagattggt accgaaaaaa
     1801 cggatccttt cacccttgcc gtcaataaag tgcccgtctt taacagtgac aaagccggag
     1861 gcccctgcca cggattcatt cagaaacgac atgtcaatgt cattactaag tgagtcggta
     1921 tcaggaatta caaaatcaaa ccatccgtat tcacggacat tattcccggt attccggcac
     1981 ccgtttgtta caaaaaggat gaacacggga agtgcaattc ttaaaatacc gggaaacaat
     2041 gaagcagtaa ttttcatttt attgattttt aagttatccg gagttctgac aaattaatca
     2101 aagtacaatt taaacaatac atgtggaata aatccattaa aacggatccg aattgcagaa
     2161 atatcagcca aaagaaaact gcatgattta tcccgtgacc ccggaccgca gttgcggttg
     2221 caggaaaggt acggaacaaa gattatgctc cccttttgac tttaattgtg aagcagcatg
     2281 caactatcag aaaaaaataa aatcaggaac cagaacagct tgtggtttta acacaggtca
     2341 tggcaggcag gttcctgttc actgttttcc ggtttgcccg ggtggcttac ctgttttctg
     2401 atgatctttt caggtcggtc agccttccgt cgttgtatat tacaaggttg ctttcctcgt
     2461 tgcctttagg gaaataacac tcaaatgttg tccgtgtccg ttctatccgg ggggttggaa
     2521 cattttccgg aaaatccgat attttcagac ccagatcctt taaacacgaa gtatattttc
     2581 cgtatttctt aaaatattct ttctcggcat aataaatcat cctgagagcc catttccgtc
     2641 cgagatcccg gtcgggaatg aagtcttcgg ttcccgcccc ggcggtcagg cctgaaaact
     2701 ggaggtatcc ccacatctca ggcatatgca tatttatcct tccctgcggc gaccacaccc
     2761 agttatcttc agggaaaggc cgttttgttt ccggattgat ttccttccgg tattttccgt
     2821 tttcgatcac ggcccgccat tcaaccctcg aaaaattaat tctccactga tccccgggct
     2881 ccggtaatcc tgctatcccg gcacattccc gaagggctgc aaggggcaaa acgatctcaa
     2941 cactccatcc cctgtctgta tcatccggct tatttatcgt tccgtccaca tgggttcctg
     3001 tcctgagtcc cttgatatcc cagccattaa tcgggaaccc tccatcgtcg cgatacggtt
     3061 ttgtcagcag aaggtcccat tccgtaccaa aggcatttac ttcaagctca tagtaatttt
     3121 gtgtatcacc atcaggatca atgaacacct caaaatcatt gtcaatgaaa atcaccgtat
     3181 ccctttgcct gagtgttgcc catacatgag gttcctcaag ttcagcggca atgtaaaggt
     3241 tggaattatc ccaaagcatt ttgacacgtg ttttatatgc cggcgcaggt tttgcatcac
     3301 cttcgatatc aacgaaatag tctgaccatg gtgcagcatt ccatgaaggt tcatcaagaa
     3361 taccgtcggg ttttatcgga gatgcggtac ggtagcacac ataccccttg ggatcgggtt
     3421 ccggtggaat gacggaggca gtcatttccc cgcttatggc tgatcttttg catgcccctg
     3481 caagaatcag tattgcaagg aacacggacc atttgataag gccgttgcac ttggggatag
     3541 ctgtattttt cattttcatg tcaggttgtt tatttaagat tgttacagaa ccaggtaaat
     3601 caaatttaat cttttaacat gatattctga ccggggtttg ttaaaaacat tccagatcac
     3661 aattcaatca ggatttttca atatcttgta tgtggcaaga tttcttaatc tgtcttcttg
     3721 cccgacacat tcatcagaaa acctgttcat ctgattaaaa cttaaagtat atggaaccat
     3781 catcttattc aaggcgcaaa ttcctgtctg ccggctcagt tctggcagcg tcaacggttg
     3841 cagcatctgc agccgttccg gcaggcccgg tgtattttac cgccgcaccg ggaatgttaa
     3901 atgcaaaaga cttcggcgca aggggggacg gcaaagccga tgacactgca gcgatacaga
     3961 aagccctgga tactgcaggg ccggtcaatg gtgcagtgat gatccccgaa ggcaactacc
     4021 tgtgttctga gttaaaggtg ccggaaggca ttgggattta tggcatgccg gcttggagct
     4081 tcaggagagg catgggcacc ataatcacgt ttaacggcaa aggcaggtgc cttataaacc
     4141 tgacaggcgc ctttggggcc tacctctctg gcttgtgtct caacggaaaa aacattggag
     4201 aaaatatcca cggtattctt attgacaagc ctgattacgg tacgcaggaa gacacaccga
     4261 ggatagatac atgcagggtt gaaagattca cgggagatgg cataaggctc gagaggatat
     4321 ggtgtttttg cgtaaggcat tcccatctga tcaataataa aggatgcgga ttaagggtac
     4381 gcggatggga tggattcatc ctcgacaact ggttttccgg gaacggcaag gctggctatg
     4441 gctcctatga tgaaaatgca tccaacacca tgactggcaa tcgcattgaa tggaaccggg
     4501 aaggcgggat agtcattttc ggcggatcgc attacaatat aacaggtaat tacattgacc
     4561 gttcaggcag atatggactt gccctgctgc ccggtccggg cgggaggaac tgcgaggtca
     4621 tcactgcaac ggggaatgtt atctacagga gcggaaaacc cgaatggggc agacaggatg
     4681 attatgacag ttcccatgtc cggcttgaat cggtaaaagg tcttgttttc acgggcaatg
     4741 ccctgcattc gggaagggat gacggtggta agggcagctt ttcccccgat tatgggatcg
     4801 tcctgaaaaa tcttgaaagc agtatagtaa aagacaatgt cctgcataac ggggcgctca
     4861 gggaactaat ggccgatctc ggcggccatg gtgaaggggt tatcatcagg gacaatgtcg
     4921 gttcattgag aaaattatcc taacacccgt actatgaaca ggaatattct gatccatata
     4981 atggtatttg ccttttcgct gggtacatat gcacagtcct ctacgctcct gctccacagc
     5041 aactggaaag cgaaaaaggc cgttgacgtc ctggttgacg gaaccatgat aaccgggact
     5101 gatttcaccc cggtaggatg gatggatgca gtggttccgg gaactgtact gaccactctt
     5161 ctccacaatg acctgatccc cgatcctttc ttcggcatga ataacgacct tatccccgat
     5221 gtatacgata ccggcaggga tcattataca tactggttct ataatgaatt tgagatgccg
     5281 gaaacaggtg aggacaggga agtatggctc aatttcaggg gaataaacta tttcgcggat
     5341 atttatctca acgggaacag ggtgaataca acggttcatg caggaatgta tctcagggaa
     5401 aaatacagga tcacgcctta tctttcaagg ggaggaaaaa acaggctggc cgtcctcgtt
     5461 gctccgcctg atccggttgg caacgctgct gccgggcagg gaggggatgg aaccattggc
     5521 cgcaatgtaa cgatgcagtt cactgccgga tgggactgga tctgccctgt aagggaccgc
     5581 aatacgggga tctgggacca ggtaagcatc gagaccactg ggaaggttga tctgaaggat
     5641 gtttttattg aaacgaaagt cccgggaaca agggtgcccg gcgcaaaaca ggatcctgct
     5701 tttgttaaag tgtcagcaga gctgaaaaac acatcggaag ttgcaataaa cggaatatta
     5761 agggttgagt tcaagggata cgtaaacaag gttaaggtta ttcttccgcc ttcggaagaa
     5821 aagaatgtca caataggcag catcaaggtt gaggatccgg aactgtggtg gcctaacggt
     5881 atgggtgacc agccgcttta ccggatgaag atcgattttc tgcaaactga cggcacactg
     5941 ctggattccg aaactgtaac ctttggtata agggaaacct caaacacttt tgacgaaaag
     6001 ataaaaggac aggttttcaa ggtcaacgga cagaaggttt ttatcaaggg aggcaactgg
     6061 atcgcttccg atgctcttat gcgcctttca aaggaaagat atgaggccga ggtccgtatg
     6121 catgccggaa tgaacatgaa tatgataagg gtgtggggag gaggcctcac tgaacgaccg
     6181 gagttttacg atgcatgcga taaatacgga atactggtat ggcaggatgt ctggatatcc
     6241 ggagattgca acggggaatg ggatgaccct ctgaaaaagg aatcacaggc acggagaaaa
     6301 gcctatcccg atgatcatga cctgttcctc agatcggtta ccgaccaggt aaaaatgctg
     6361 agaaatcatc ccagtcttta tctatggtgc ggcggaaatg aaacctaccc tccggccgat
     6421 attctgaggg ccctgaagga agaaatcttt ccccgctgcg atccgggaag attttttctc
     6481 gacatgtcaa catcaccggc cctgatgaca aatacaatag gcggagtggg tgacggaccg
     6541 tacggcataa gggaaccgga aaagatcttc accgaaaggt ccttcccctt caatcctgaa
     6601 acaggatcga taggcatccc gaattatgac ggtctggtaa aaataattcc cgaagatgaa
     6661 atgacacctc cgggagccac cggccggcct tcaaaatcat ggacatatca caaatacctg
     6721 ccattgtatg atttccccga ccgttacggg aaagtgaagg atgtgcgtga tttctgcctt
     6781 aaggcacaga ttgtgagtta tgaacaatac agggccctgc aggaggcatt caactataag
     6841 atgtgggact ggtacacggg aatgctggta tggaaaaacc agaatccctg gaccgcactg
     6901 aggggatttt tctatgatta ctatctcgac tacacagggg gatacttcgg atacatgcat
     6961 ggtgctgcgc ctgtacatat acaactgaat ctcaacgact cggttgtatg caccctcaat
     7021 cagaccgccc gcagcataaa ggatctggaa gcagaggtat tgctttccga cctgcacgga
     7081 aaacaggttt tctcaaggaa aagcacatta gacctcaacg cccaggaaac aaagctgctt
     7141 gacaaggtgg agctgcccgg ggaagacagt gaagtgttct ttcttaaatt acggcttagg
     7201 aaaaacaacg agattattga tgagaacctc tactggctca caaataagcc aaaatcatat
     7261 gaaaagctga atgatctccg aaacgttacc ctgaaaacgg atgtcagcag aaccggtcag
     7321 aagaatgcaa cagtttcagt cattaatcag gaaaatgaga ctgctttctt catcaggctg
     7381 aagattgtcg acaggcaagg caatcttgtc cttccatcct atttttcgga aaattatttt
     7441 acgttgctgc ccggagattc aaaacaggta aatattgagc ttccggcaca accgccgacc
     7501 aggaaaagtg acgggatcag gcttgtgctt gaaggatgga acaccggccc tgaagagatc
     7561 agcttctgat acccgggcta ccgctaatct tcaagcaatc cgggtataca ggcaatcctg
     7621 ccggtggcac tgaggcagct gtatgtcctt aaagcaataa atacaacttt atctgtcagg
     7681 tttctgaata accgtcgtca tctccctgtc aagcaacata cccctgtatt cctcatagga
     7741 acagtcgaaa cgaccgctgt ttattattcc catcgttccg atcaggttcc acattgcata
     7801 acctgttttg ttgcggttca aaattgacac gacatctgaa aggtaggcaa gtgcaacaga
     7861 atgcggtgtc ttattaaata caccaaattc ctgtaccatc acaggtatat tttcttcacc
     7921 ggccatcttc aaggcattgt tgaggctgga aagagcgaca accggattgc ccccggtgtc
     7981 ggttatcttt ctttctgtag tgattctgta ggtattctgc cttgcccccc atgttgtatt
     8041 ggcgggaata atcacgatac tgtctgttgc gccgaacctg attgttattt tattaacagt
     8101 gagccagtcg ccgtcagtat tcgagaaggc cagccttgtc ccgcccgaag gcagcacagc
     8161 cgtataatct tttcctgaaa tattctggta accccactgg gtttctatta tcctggtcca
     8221 gtcatcaccc ggatcgggcc cgcagatgaa cacttttttg tacacttcat tttcatccag
     8281 cctgatctga agtgttgagc gaaccgagac ctgatgtaca ttcacggtaa tttccgtgcc
     8341 ccccgggaaa tttccttcaa aaatcaggga tgaagaatat tcaggtttaa ccggcccgta
     8401 gagataccag gagatatcgg tcattggcca gaccggtagc ggccatgaat cggaacccgg
     8461 aatccagtcg gccctgtaat gggtaagggt tacgggatca taaacatgaa tcgcctgaat
     8521 gatatttttc atgcccctga aggagggaac aaggtcacgc ccgatattca gaccgtcaac
     8581 aaaaatgatc ctgtcgggat tgatttgccg gatcctgtcg atagccattt tcattaccga
     8641 aaaatatgta ctttcatcga cgtcacccgg ctcattgacc aggttgaagc tcagatcggt
     8701 ccagggcaca ttcctgtaac gttccgcaaa aaactcccag tgttttacga atgccgcccg
     8761 ggccacagga tctgtccaca ggtcaagatc ctggtttgca ggcaggattg tcttgtttac
     8821 acaatatccc ggcgccctgt gcaggcatat gcacacatgg acgccgtatt ttcttcccca
     8881 ttcaactgca tcatctattt ctgaacatcc ctccgaaaat tcattccagt ttcctgcctt
     8941 tgtataagtt ctgtaatcga gcggcaacct tgcaaaatta aatccaaggt cctttattat
     9001 ttcaaactcc ttctcactga agcctgtatt tgaccagccg gcatcaaact tgcccaggag
     9061 gttgaatccc ttgtgagtct ggctgaaaag attgagatca attggcttga aaacgggagg
     9121 tgcttcttta tcttcactgc atcctgcggc aaaaagaagc aggacgcatg aaagcatcat
     9181 ggcgttaaag ccttttgcaa attgcagcaa cgggagactc ctgcctttca tgggaccttt
     9241 gccattgatc tctgatccgt tgacaaagat agaaaaaaca ctccggaata ttctctgacg
     9301 aagaacagtt aaagatgcac catcatcatg cagttttgcc gatcattcat atcagggcat
     9361 ttcaacctga tccggctctt ttcatttctc tttaaaaaat tttagaaatc agatatacta
     9421 ttttatcctt aatttaagtt ataattgcat caactttaaa actattttaa tgctatgaaa
     9481 aagaaaattt cctttttagt actatctcta ttgattatta tcgcatcctg cagcaaaacc
     9541 ggggagaagc ttggcggctc accatccccc ataggcgagg aaggtgttac cttcagcaca
     9601 tcatcgggta cgattgcagg agtaagcaac gttagcgctt cggtaacgga actgaaaggc
     9661 ggtgtttctt caataagcgg tagtgctgtc gtaacaaatc ctgtcatcaa aaacattctt
     9721 tcaaatcatc ctgaggctca aatcaacggt aataatgtta cggtcaccga tgtggaattc
     9781 aggatcacaa ctgaaggcgt tgagtctgta tatggccttc agccgggaat aatcgtcaaa
     9841 tacgatgcaa aagtgggaga taaatacaag gttggcgatg gtatcacaag ggaagttgtc
     9901 gcccgatcga ccgacaatga ttatttctgg ggaggaatgc ttatcaaggt cattcaggtt
     9961 gaggaaaata ccaataagtt cggcgtaaaa aagataacct actgggccaa tcaccggttt
    10021 ggccttgtcg gaatggaatt tcagtttgac gacaattcaa ctgcaaaatt cccgatcttt
    10081 gccagcgccc agaacaactg ataaaacaat ttccctggcc gggtagtaat tatgtttaaa
    10141 tatcttacga tcaatggtga aaggaaaagg atcccttcct atccccatca taaatacttc
    10201 gtttgggcaa gttacaaata cttcagtgta aggagtttat tgggctttct tacccttgag
    10261 ggtcctttgt tcattccaag gcggatttac aataacctga aggcacggtg tgttgttccc
    10321 ctggtactga tgatagatcc gacgagttca tgcaatctca gatgtaccgg atgctgggcg
    10381 gccgactatc agaaaaatag caatctttca tttgaaaagc tcgatgaaac tatcggtgag
    10441 gctgtcaggc tgggggttcg ccagatcttc atgaccgggg gggagccgct tatgagaaaa
    10501 ggcgagatcc ttgaactgtg ccgcaggcat aagggcatac ctttcgggat atttacaaac
    10561 ggcactctca tcgatgaaaa tctggcaact gaagctgcca gggccggaaa tctgaatttt
    10621 ttcataagca ttgaaggttt cggcgaggaa accgatatga gaagaggcga tgggacattc
    10681 gacaaggctg tcagggccat ggacctgctg aaaaagcatg atattgcctt tggtttttca
    10741 gcctgttatc attccaaaaa ctaccgctct gttacaagcg atgagttcct cgattttgtg
    10801 cggagcaagg gcgcctggtt tggatggatg ttcaactatt ttccgatagg agatgatgcg
    10861 gacctgacac tatgctgcaa accggaggaa agggcttatg caaaggaaaa aattgaggca
    10921 tactgcaaaa aacacaatta caacattatc gattttgcca acagcggtca taagtcgatc
    10981 ggttgtgttg ccgctgccaa tgatttcgct cacataaatg ccaacggcga ccttgaacca
    11041 tgtgctttcc tgcactattc cgatgtgaat atcaatgaca tgccgcttac ccaggcacta
    11101 aggtcacctt tctttaaaaa attcaggaag cataagccgt tttcgaagaa tatgctccgc
    11161 ccgtgtccta taatggatgt tcctgaggca ctgacaaaga taaccgacct ggaaggggtt
    11221 cattctactc atatgcgctg cccggaaacg gcagccaggc ttgctgaaaa aactatcccc
    11281 ctggcagaag cctggaaacc cgtggccgaa aaaatgtttg agggaatgcc cgcagcagaa
    11341 aaaagacggt tcggcatact gaactggctg ctgcaatcgg gtaacaggat gatttcagga
    11401 taaaaggatt aaatcagaaa ccggggatca tatcacaaga tgctgtcgtt caaatggaaa
    11461 attgccggct atactattgc agtgctgtct gcgatcctga cggttctgta ttttgcggcc
    11521 ggtttcaggt ttgagatacc ggtgtttgcg attgtgtctt catacaggga aataaagttc
    11581 ctttcaacct tcagcaccaa ttttgccgat gaaactatcc tgctgggatt catttctgga
    11641 tttgcactga tcgctttctc taaggaaaga aaggaactgg atatttacag ggaaataagg
    11701 gtaaatgcgc tgattaaggc tgttgtcatt tacaccctgt tccttattat ctctgtcctt
    11761 ttcctgtatg gcagcgtatt catgggaata gctatcctga atctggtatt gccttttgta
    11821 ttatatatac tgattttcaa tattctgaaa ttcagggcac aaagagaacc ataagggagc
    11881 cgggattttc agctgcagca gatcggcaga gatgcctgac ctgagtgaaa ttaccaatca
    11941 atgcgtccgg ccggatttat gccaattgtc atactcctgc aattctgcgc ttgtattcgg
    12001 ccagggcctc atcgagtctt tgctttgcgg tgccatcgcc gggaacattg tgagacggat
    12061 gaatgtaaag tttcactccc ctgccggcaa gtatctcggc aacggttgtg accgtcatcc
    12121 agacgcaggt aacgaatgcc attgtagaaa caggaccgat cttgtcctga tgatctttca
    12181 gtatgactga tgcatcttcg aggggggcac atgtatcgac cacgacatct gcaagttcaa
    12241 aaatggtttt cccgcacgaa tgcctggtct tcttcccttt tgctgcctct gccgatccga
    12301 atactatgac cttcatgcct ctttttcttg attcaagggc aatatcaatg ttcacgttat
    12361 tgatgcctga atggctgaaa agccacataa tatccctctt gtcaaaatca taacccttca
    12421 ttatttcatt gccgtatcct tcaacacgtt caaggaacac aaactgatgc acccccattt
    12481 cgccggttat cctggtaaaa aaggtcagcg gcagttcaat catgggatgg aatcctacaa
    12541 atccgccaat gcggggatac atttcctcaa tcgggagggt tgcatgacca cagccaaaag
    12601 tatgaaccca tcttttacat tcaattgtgt cagccatcag ttcagctgct ttgcggatat
    12661 tgtccatctg tgtttcctcg atccgggtca ttacggccct ggcgttctca agccattgtt
    12721 ttgccagcat agtatattaa agtttaagtg ttgataaaag actaattgaa aatttcaatg
    12781 cgtgaaaata ttttattttt ttctaaaacg gaattcagga aatataattt accctattaa
    12841 ggttcacatc aggccggcat gcttttatct ttcttcagct taaggatgca gaacaaaagg
    12901 gctatcatta ttattgtttc gcaaaatgcg acagtaatca gatgacttat tccgaaattt
    12961 ttcgaaatta tgcccatagc gtaatttata agcatatttc cgagaagggc aataaataat
    13021 acgaagctga aggcagtgcc tgaaagatcg gcgtaggcct ctcctacaaa tccgagcatt
    13081 acgggaaagc ctcctgccag gcccgctccc aaaagaaaaa agcccgtcat caggaaaggc
    13141 aggatctccc ctgtcctgat aatgatcaat cctgcaataa tcattgcaaa tgatatcacc
    13201 aggatcctgc ccacaggaac gtttcgcaat gcggttccgg taacaagacg cattatggcc
    13261 atgcctgcaa cgaacatgga tagggtaaac agtgcgctgc cttgcggaat atgagcctga
    13321 tcaataaggt aggtggtggt ccagttgttt attattccct cgaagctgct ttgaaagaaa
    13381 aggaaaaaag ccacaaggat aataaatctg tcattaagga aacggaggct tttccctagg
    13441 ggaaatccct gcatctgttt aggctggggc atctttatca gcaggaacag tattccggtg
    13501 gcaaatgata acagtccgat gaaggcgatt atctggcccg agctgaatga tttttcaagg
    13561 atgccgagca gcatgggcat gccaagtgcc ccgatcccga agaatactcc cagaaggctg
    13621 agatttgcac ctttgtcgcg gtcacttatg tcggaaacca gtgcatttgt tgcaccgttt
    13681 attgaaccgc cgctgaatcc gacaaggaag atgcagacct taagcaatcc ggtcccctgc
    13741 gaaaaggcaa taccctcgaa cccggcggcc agcagaatac aggataatgc aagaagtacc
    13801 ctgtaaccat acctgtcggc aattggtccg aataccaggg atccggcaag gatgccgaag
    13861 ggaaggattg aaaacagggt tccggaagca atctcatcca gtctgaattt ctccctcagg
    13921 tcgggggcca ggctgccaag tgaaataagg gctatcccga acaaaaatat gccaaggcat
    13981 gctgaccgga aaactacttt tttattatac atgctggtat aatgcgtttt attggttttc
    14041 agcgtttctc agggcaagac ggccagcgcc ataaacacct gcatcacccc ctaggcccga
    14101 aacgtcaata ctgacgcggg caaaactgac aggctgggcc catctggcag ctgcttccgc
    14161 gatgtcgggg aggagtgaaa ctgccggacc aaaaactccg cctccgaaaa ttattttttc
    14221 cggatcaaac aggctcacga aatttgcaac tgccattccc cagtattcca cagcttcttt
    14281 tataaccatc accgcaactt tgtcaccctt atcgtaggcc tcaaagactt cacgggatgt
    14341 gatttcatcc ccggctgctt ttcccaatat gctgtcatat tccttatggc cgctcagaat
    14401 ttccctggcc accatggcga tcccgcttcc cgaagcatgg tattcaaaac atccgcaagc
    14461 tgcatactct tcccggaacg gccggttcaa tgccatccat cccgttgaac cagcaatatc
    14521 attgctgccg cgtaatattt ccccatttac cattatacct gccccgatgc ccgttcccac
    14581 tgacaggaaa acagcatccc tgcaacccct ggcatttccc ttccatacct caccaaggat
    14641 ataacaggcg cggtcactgt cgacggccac cggtatcccc cctgaaactg aacttacctc
    14701 tgacagcagc ggataatcgg tccactcctc aatgttagga gcccataccc tccccgtttt
    14761 cgaactgaag ataccgggaa ctgaaatgcc aatcgacctg atgtcagtac cttgtgataa
    14821 agatctgtca atcagatcaa caatggtacc ggtaataagg cttcccacct ccgggccttt
    14881 tctctttcct attgcaactg tttccttgac cagtaaatcg ccattttccg aaaagatggc
    14941 acaggcaagc ttggttcccc caagatcaat tcccaggaca ttcataaaaa tccgcttttc
    15001 agtgtttaat cagttcttca gaagttcatc tattcttacg aaaacatatc cacggttgat
    15061 caacgtgccg ataaggtcag gcaaaaagga ataaaattta tcggtacgac gcggatcagt
    15121 tcctatgtgg accaggagga taaagccttt taagccgttt gatgagaact tttcatagtc
    15181 gagaacagat ttcaggatac acctgctgtc gaggtacctg tcgcccatct cgggataagt
    15241 gtaatctgca ttgcttctgg tgcccgggct gaaattgatc agcacaagtc ccatccggtt
    15301 cgtccaggat gctatcgtgt cattgtacca ttcgtagggt ggcataaaat accgcgatcc
    15361 ggcctggtca atattgaatt tcttcatttc ggcataggaa tcctcaagat ccctgacaaa
    15421 ctcttccctt gttacaagca ggctgtctct cttttcccag tcgcaataca gcaggtgcct
    15481 gtctgaatgg gatccgagat agtttccgtc gctgaccagt cttttaatca agggatggaa
    15541 catcttattc ctgtaaaagt tgcctgtgaa gaaaaatgac cctttgatat tcttctccct
    15601 tagggtctca gcaatgaaat cacctccgtc ggcgaatgaa tcacccgtga aaacaagtgc
    15661 gatcatgtcc gctgtggtat cgcccctgat cactgctccc gattcataga caaaaccggg
    15721 gcgaacctgt tgcttcgcag ttttcacgga agtttctgat ttactgttat tcgtattgtc
    15781 tgaagtattg cctttgttcc ggcatgaaac cgccagtacc agtaaaaaca ataagttcag
    15841 aaagatccgg ggataatttg ccataatctg tatttttaat tagtcagaat ttctccatta
    15901 agaacaacag tactgacatt aaagtcttta tcgaaggaga ccaggtcggc tctcatgccg
    15961 ggttttattt ttcccctgtc gtggagattc atcagcctgg ccggatacag cgatgccatt
    16021 cttattgctt catcgaggtt tataccagtt ttttcaacac aattccttac agctgtaagc
    16081 attgtaagtg ccgatcccga caaggttccg tcgggaaggg tgaacctgtc ttttctcctt
    16141 atgtgcggat tggtgggctg cgaactttcc tcaacagcat cagaaaccag gaaaaggcga
    16201 tcattcatta tcttcttggc aatggaaacc atggtgtaat caacatgtat tccgtcagca
    16261 atgatgctgg ccataacatt gccgttcatg aaagttgctc cgggcaggcc ggggtcacga
    16321 tggtgcagct gcgacatggc attgaaaaga tgggtggtgg tccggatccc ccacataaac
    16381 ccccgggtgg cctcattgta ggttgcattg ctatgtcctg cagccacaac cacattatgc
    16441 ccgttcaggc attcaattat ctcccggtca cacagctccg gtgccactgt catcattctt
    16501 accacacccc ccagatcctt catcagctga tctatttcgt ccctgtcggg ttttttgatg
    16561 cagctcagga aatgagcgcc gcgccttttt atgttaagat atggtccctc caggtgaagt
    16621 cccagcacgg agggaagagg attatccctg atcacggcag ctgcctcatt gtataccgcg
    16681 aaggtgttcg tgggtattgc tatcaggaat ccggtggttc ctgtcattac gatggaaccg
    16741 gcaatggtcc tgagcgcctc ggcagtggga gtctccgaga acaggaagcc gccgctgccg
    16801 gcaatctgca gatcgatcag tccgggagca atataatgcc catggcagtc aattatattg
    16861 atatctggtg gtaaatgaga gggctctttt attgcgatta tcctgtctcc ttcaatcagg
    16921 agggccgctc cttcaattgt ctcctctccg gtaatagtta ccgcatttaa aaaagcagta
    16981 gtcatcaaaa ctgaaataaa gtgatcttaa tatccttttg cagcaggagt ttcaactgtc
    17041 ttcacattcc atcccgaaat ccttaaaacc ggattgttat ctttcagtcc ggcctgcgga
    17101 caagtcacat taacaacagt gctttccccg ggtgcgaggg aaaaataatt acgcgaccag
    17161 agtgaaggaa gtacctcttc tccgccggta acgatctggc ttcttatgaa aaaggccagc
    17221 cggctgccag gattgctaac ttccacggtc catgaaatat cacttccggt tttcctctca
    17281 tcagttatcc tgacatttac gggtgtttcc tgcatctcgc tcatcgggct gtaattttta
    17341 tcgggagaga tccagtaaat gttactggaa ataactttcc ccgatgcatc tttaagtgaa
    17401 agaacaataa aaacaagttc cttcatcttt gcgagccgtt gtccgacaga cgaggtttct
    17461 tttacatccg tcggcccgag gcttactttt ttctcttcat ggaaaagcag ttccgatttc
    17521 atattgtaaa ggtcaacgca tgaaacaaga cccggagcgg gtttatatgt ccgattgagc
    17581 acggtaaccc tgttgtttgc cagattgagc tgaatatgga ccggctcgca tgcattctgc
    17641 atgaaataat aacctgcgtt tgtctgcagg taccagtcat acacctgcca taccacactg
    17701 gggaatgcag cattcagctt ccagagcatt acaccgccaa tatcattgag tttatgcccg
    17761 gcggcctcga atattccctg gtagcctatg gcattcataa gctgcatctt attgcaaaaa
    17821 tcctccatcg acacgggttt tccgtatctt cttatcatct catgataata caggtcccag
    17881 cggccattgc ccgttgcggc atcgtgatat ccccaggtgt ggttcagggg aaaaggcagt
    17941 gtcctgtccc ataccagatc cgggattatt ttatgcagga tgtcatatgg aggcattgaa
    18001 ggaatcccgg tttcatcctt aaagacccaa tcctttgcct caattgcctt tttgtagtat
    18061 tcagcgggat cttgccatgt gtacggtccg ccgctgtaaa ctccgcccgc ctggttatca
    18121 ggccaggagc cttcccatcc gtcagggatt ttggcaaatc ccgacgaact gggaataaag
    18181 ggcctcgttc cgtcgagcct gatgacactg ttgcgcataa aatcataaag ctcctttctc
    18241 gcatgcccct cattgcctcc ggtccacacc aggagactgg gatggttcct gatccgcagg
    18301 atggtactct caacatttct tttgaataca tcgctttcca atggccagtc aggcgaaccc
    18361 ttgaattcgc cctgggtatc acccgttatc cagaaatcgg accatacaag caatccgtat
    18421 ctgtcggctg cttcaaaaaa ctcgtcacgc ggggtgaccc ctccgcccca tatccttacc
    18481 aggtttatat tggcattgcg gcaaagcctc agttcatagt cataccgaag ggaatcgcgg
    18541 tttaccataa aatcaggaac ccaggctcct ccagtcacat ggatccttct gccattgaca
    18601 taaaaatcac ggcgaaggct cccttttaca tccacaggtt ttgacgacac tgttcttatt
    18661 ccgaaaagga aagtggtgtc atcagatatt cccgactcat ttgaaaactg cagtcgtatc
    18721 ctgtacaggt ttggctttcc gtatccgttc ggccaccaca gtgcggggtt ctcaatcaga
    18781 agttccctgg tgttaccggg atccagttca acctggttcc gtcctccccc gcttaccgta
    18841 atgctttttg agaactgaac ggatcttccc gtgaaatttt ccggcgagat ctttactgtc
    18901 aggtcgcctc tttcattgtt gttcccatga ttgaacaggg tcatactgag ggagatgtgc
    18961 gcagcggagg tatcctgtcc gtcagcaaac cggatatcga tccgcggatc gccaattgtt
    19021 acattgcctg aagtcctgag ataaaccggc agccatatac ccatattcct gtcgcgtacg
    19081 ggaggcaccc agtcccaccc gactgaacag gccatggtta cattttttcc gatatcaccg
    19141 gttggccccc cgttcagata aaagtcgccc agagcctcaa gctgttcggt atcaggcagt
    19201 ccggggtaat ccagcggata gatcttcacc gcaagtgcat tttttccccc ggccctgatc
    19261 tgtttgctga catcgaggga gtattgggca aacatacctg ccatttcggt tgagtctgca
    19321 atctgcaggc catttaccca cactgcagcc cgatagttaa tacctttgaa aattaactgg
    19381 aaacaccttc ccttgtcggt tgccggaaca tcaaacgtgg tcctgtacca atatggcttt
    19441 ttccagggat ttggttcatt cggcaaataa ctgtattgct ccaggttgta tcttttattg
    19501 aactcgtccg acgcatccgg gatcagcata ttgttcatcc cgttatacgg atcggggtaa
    19561 atcccgtttg cgaccagtcc tgtaagaacc gttgaaggaa ccctgacagg gaaccagtaa
    19621 acaacggacc ggtattcagg gaccgataat ttgtccccgg tttcggttat gactgccgac
    19681 gattgcagtt caaaccgttc aagcttaacc tgttttacct ttccaggctg tgaataagcc
    19741 tggtaagaga aaaacagcat caggcagatc agataattta cattcctttt cattatgatt
    19801 tgattttgat ttccggagcg atggttaaat atactgatca acggattatc aggctattcc
    19861 agggttttat tgatcagcat ccatgcatct gaatactggg gaaacatcag ccatatccgt
    19921 ctgacctcac ttcttgcctc gttctcatca tctgttgcca gcacggaaac cctgtataat
    19981 ccttcctcgg ttttaagtat ttccggcctg aatccctccc tttttgcctc tgattgccgg
    20041 ttccgtgcat ttgccggctc acggaagctt ccgacaatta caaaatattt ttcccggttg
    20101 atcttattgt catcacccgg tacaatcttt ttttcaatgc cctgaacttc cctttctccc
    20161 tcagcaggcg caacaggttt tttctcttca acgggtgcag agggcatcag tgtttttctg
    20221 gcagtggttc ccggaatgta attttcagtg ttccggctgt cactgatctg ccggttcttt
    20281 ttggtgcctg cgcacgatcc tgacaacaac agtgtgataa tcagaaagga aatcttgatt
    20341 ctcattttgg taccatttcg aatctgcagt aaaaatgcga aaattattcg agaaaaatct
    20401 gcggcaatcc ctattaataa gcaatatcaa gaatctggag aaagcttttt tcattaatca
    20461 ccctgtcggc aaccaggttc aggtaattgt tgtttgacct gaaagctatc ccaatgccgc
    20521 tctccctcac aaggcagatg tcattttcac tgtcgcccac ggaaatgata ttcttagtgt
    20581 caatattgta tttttcagcc agctgaaaaa gcacgttcga cttgcagaaa tcatgattgc
    20641 atttgctgtg cctgtttctc atgaatgccg acgggacctt tacttctccg gttgcaatgc
    20701 tctgtgaaaa ttcaagttca tttgcaacgg aaaaatccat cctgagttta tttttaagat
    20761 gatttgtgac gatatcgtaa ctatcgctga ttatgccgca gatataacct ttctttttaa
    20821 gctcgccgat aacatattcc gcatcatcaa taatcttgat tttgtcgacc acgtccagta
    20881 tctctgcaaa ggttctgttc ttcagaagcc ttgcaattga ttttgtacgg gtgtaggcat
    20941 tgctgtattc ggtcacgatc ctgatcagtt cattctcgaa attgaattct tcggcagcct
    21001 ttgttataaa actcccttcc aggagggtat tatccatgtc gaatattatc atcttttcga
    21061 gtccgataag gctttccctt attgcataat ccatctgcga ccggatgata ctaatgtttt
    21121 cgagagtctc aaggtttttt tcatcaacgt tatgaacccg tttaaggatc gcctttgaaa
    21181 cctccctcga cattttcccc agctgctccc aggtctgcat cctgttctcg atgtatccga
    21241 tattcacttc cataattctt gccccgagca gatgcatgtc tagcaggatg ccgatatctg
    21301 ccccataatc attttcaata attatttttt ccatccagct tttacgacag gcgatcatcc
    21361 cgctcagcgg ctgcgagaat ttgaccaggt caggaaaaag caggctcagc aatggttttg
    21421 caacaagttc ggtgaccctt cctgcctggc gggaaaagct tgatttaaca aaatcagcct
    21481 cgtcgtttaa caaaggtgca gttaaaagtt caatgacatt atcgggataa ctaaggatat
    21541 cagcatccag gaacacattg atctcatttt ttgaaagaag caagccgtcg cgcatcgacc
    21601 ctccctttcc caattttgga ctggtataga tctttacctt tggcagccgg gcctttttta
    21661 tggtattgtc gcttgacttg tcatcgatta caaggatctc gtccaccagg gatgacgcac
    21721 tgacaagacc gataacatgt ccaattgtct tttcctcatt taatgccgga atgattactg
    21781 taaccatttt ctggtatgct aattagttat tttgtctttt tctcctaagg ctatttcagg
    21841 tatttcttaa tattgtaaag cctgttcaat gcttcaacgg gcgtaagatt gtcgagatca
    21901 agatcaagga tctcatccct gatctgtttc aaaacaggat catccagctg gaaaaagctt
    21961 aactgcatgc cctcacggtg atcggccagc tgtgtgatcg gttttgtaag ttcctgtttt
    22021 tcatgacttt gttccagctc cttcagtatc tccccggctc tggtaacaac gctttttggc
    22081 atgcctgcca tccgtgcaac atgtatcccg aaactatgct cgctgcctcc tcgcctcagt
    22141 ttcctcagaa agatcacctt gttattgaat tccttaattg acacattgta gttcctcacc
    22201 ctcatgaacg aattttccat ctcattaagt tcatgataat gtgtggcaaa cagggttttt
    22261 gccctgaatc tgctttcatg aagatattcc accattgccc atgcaatgga aatcccgtca
    22321 taagtgcttg ttccccgtcc tatttcatcc aggagtacca ggcttctgtc cgataaatta
    22381 ttgagtatac tggctgtttc attcatctcc accatgaaag tggattctcc gaggctgatg
    22441 ttgtcggaag cccccaccct ggtgaaaatc ttgtcgacga taccaatatt tgcctcccgt
    22501 gcagggacaa aacagcctgt ctgcgccata agcactatca acgccgtctg cctgagcaat
    22561 gccgacttac ctgacatatt cgggccggta agaatgataa tctgctggtt atcagtatca
    22621 agactaagat cattgggaat atagggctcc tccggaggca gttgcctctc tattaccgga
    22681 tgtctcccgt ccctgatgtc aagccgcttt gaatcatcaa taacaggacg aacataatta
    22741 ttattgacgg aaacgcaggc aaaggaataa aggcagtcaa ttcgggcaat gaggccggca
    22801 ttaacctgaa ccggaggtat atattcgagt attgccagta ccagatcgtt gaacagcctg
    22861 gtttcaagac ccaggatctt ctcctcggca cctaatatct tgttctcata ctccttgagt
    22921 tcttcggtta tataccgttc cgcactgaca agagtctgtt tccgtatcca gtcaggcggc
    22981 accttgtccc tatgtgtgtt cctgacctcg atgtaatatc cgaaaacatt attatagctt
    23041 accttcaggg aggttattcc ggtcctttca ctttccctct gctgaaaatc tgccaggaaa
    23101 tccttgccgc tataaaggat cctcctgagg tcatcaagtt cttcagagac tccgctggcg
    23161 ataacctgac ctttaaggat ctgtacggga ggatcggcgg tgagttccct atcgatccgg
    23221 tcggcgaccg atctgcaggg attaagctga tccccgagtg ttgccagcgc cgggcaatta
    23281 ctcgtgatca gaagttcctt aagcggttcg atggccctca gggcccgttt cagttgcacc
    23341 acttccctcg gattgatctt tccgaccgcc acctttgaaa tcagcctttc cagatcgcct
    23401 atctgcctta ccagttcaga aacctctttc atcattgaag gattggcaac aaaaaaagct
    23461 acaatatcaa gccggtcgtt aatcggctgg atatccttca gcggcaatga tatccatctc
    23521 ttcaggagcc tgccccccat tggcgaaatc gtccggtcga caatatcaat aagagtactg
    23581 gcgccttcat aaggtgagtg aaagagttca aggttgcgga ttgtgaattt gtcgagccac
    23641 acataccttc cctcttcaat tctggagata cctgtaatat gacccagccg tgcatgctgc
    23701 gtgatatcga tatagtgcaa tatggcaccg gcagcaatta taccgtaggt gagattctgt
    23761 atgccgaagc cttttaagga acttgtttcg aattgtttaa gcaatctgtc gttggcagct
    23821 tcggatgtaa agatccagtc atcaagctta taggtgtaga acctgttgcc aaatgcttcc
    23881 tgaaacaaat cgcctttcct tttctcataa agaacctcct ttggctgaaa attattaagc
    23941 atctgatcta tgtattccat ggtaccttca gatgcaagga actccccggt tgaaatatcc
    24001 aggaaggcca cacctgcagc cttacggtca atatggactg cagccagaaa attattctcc
    24061 ttgtggctca ggacattctc attcagtgaa acgccgggtg tgacaagttc ggtaattccc
    24121 cgctttacta tcttttttgc aagtttggga tcttcaagct gttcgcagat tgcaacccgc
    24181 tggcctgccc tgacaagccg gggcagatag gtatccagcg catgatacgg aaacccggca
    24241 agttcgacga acgatgcgga gccatttgcc ctcctggtaa gggttatgcc gagtatttcc
    24301 gatgctttta cggcatcctc gccaaatgtc tcatagaaat cccctaccct gaaaaggagt
    24361 atcgcgtcag gatgtttcga cttgacattg taatactgct tcatcagcgg tgtttcagca
    24421 tacttctgtt cttcggggtt gaacatctaa agcagggaaa attaatatca ttgatgatga
    24481 acaaagataa ttcatttttt tcaggtgctg aagaattact gccggctccg gcaatttatt
    24541 gattctccgg tgaaagccgt cctgctgtca atgtgttcag tgtgcctcag aacttcaggt
    24601 aaaaatccct ggtaaggctg acatattcat cagtatagtg atgtctcctt ccggtttcga
    24661 ttgtgagaaa ctgttcccgg gcttttcccg ttttcctgct caatgtcagt atcagtcgtc
    24721 ttattggggc agttggtaca ggccttattt taacgatatg attgcaatag aaaccatgcg
    24781 tctgtgcttc ggcaataaaa acatttcctt cagggtatgg aagaataagc tgcagcaacc
    24841 cttcttcctt caatagtctg tccgctcctt caagtaaatc cctgtgcgac aggctgtcgt
    24901 tgtgtcttgt tattgccctg accggatcag gatttctcag tgaatcaata aaatagggag
    24961 ggttggttac aatgagatcg aatttctcac cttccggttc aaaatcctga aggcggcagt
    25021 tatgaacctg tattcttccg gcccatctgc tgtgcaatac attttctgct gcatcggtga
    25081 aggatcccct gtcaggctca attgcaacaa tttcagcatc cgatctctgg gcaaggatca
    25141 gggcgatgag ccctgttccg gttccaacgt caagaatttt ccttctgcca tttacctctg
    25201 caaatgcacc cagcagcact ccgtcggttc ctaccttgaa ggcggacctt tcctgattta
    25261 ttgtaaactg cctgaatgta aaatagttat tggacattct gaagaaactg gtatctgaat
    25321 gatatcaata tgtcagccaa tataatcagt aatgatacga attttaaaga cagaccggca
    25381 tattaatcac cggcactggc agccggcacg tatctccggg aaatcgggaa cgggaatacg
    25441 gcagtcagtt attgaaataa tctgtcgttt ttccagcccc gtttttgata aataattccg
    25501 gaatgattat gtacatttac cggtatcctt ttaaacaccc cgatgaatta cggaaccagg
    25561 gaaatgaaag gccatccgta tgatttacgc gcgtaactct caatccatga aaagcaaccc
    25621 tgaaggaaac gatcctgata taccggtcag agccagttta cggaattata caaaattccg
    25681 gtccggagaa ttacccggaa agaacatcct tgcaattttc cgtaaggcta aatgtcaggc
    25741 tgccggaata ccggggataa aaacaacccg ggaaatgacc ggatcatacc ttactaccaa
    25801 tgtccaataa attatccgga aacccgatca cgacaatacc ggaaaccgga aatcggcccg
    25861 cgttcagaaa ctaccgatgg atcatagttg ttttgttgtt ttttgcaaca accataaatt
    25921 acatcgaccg tcagatcata ggtttgctca agccccttct cgatgttgag ttcagctgga
    25981 cggagaccga ctttgcgcga attgtgatgg catttaccgc agcatatgca attggcttgc
    26041 ttattatagg ccggtttatt gacaaaacag gaacaaaagc gggttactct gtaataatca
    26101 gtctatggag catagcaggg atgcttcatg cagttgccag gagtgtcttt cagttcgggc
    26161 tggccaggtt tgccctgggg ctcggggaat cgggcaactt tccggccggt gtaaaatcaa
    26221 ttgctgagtg gtttccccgg aaggaaaggg gactggccac cgggatattc aattccggaa
    26281 ctgccgtagg cgttgtgctg gccctggtta tcgtcccatg gatcctgcgg catttcaact
    26341 ggcacggggt attctggatc accggggctc tgggattcgt atggctcatt ttctggttga
    26401 tctattacga tcatcccgca aaacagaaac ggctttcttc aggcgaactc aaacatatca
    26461 ttgagggaca ggcaaatgaa gaacaaccga aagcgggttt aaaatggcac agactttttg
    26521 cctttccaca gacctgggct cttataacag ggaaagggct cattgatccg atattctggt
    26581 tttttctttt ctggctgccc tcatatttct cttcggcata tgaacttgat ctgaaaagac
    26641 ccagtcttga actcatcatt atttacactg caacaaccgt cggcagcatc ctgggaggtt
    26701 atatttcatc cgtcctggta aaaaagggat ggcccgtgct gaaagccagg aaggcttcgc
    26761 tcctggcctt tgccgtcatg gagttgtcca tactgctggc acaatacacc acggccgcat
    26821 ggatggcagt cgcgatcata agccttgccg ttgcggttca ccaggcctgg gcaacaaatg
    26881 tgtttacaat ggcttctgac atgttcccca gcgaagctgt cagctcggtg gttggaattg
    26941 caggaatggc aggcgctgtc ggagggatac tgtttcccat ccttgtaggt tatctgctcg
    27001 acacctacaa ggctgcaggc aatctgaccg gaggttacaa ccttcttttc acaatctgcg
    27061 ggctcaccta ccttaccgct tggaccgtca tgcacctcct tacacgcaaa catgcatcag
    27121 tcagtcttgg tgagcttcgg taataaattt ttaaataatc cggtacaata aactatgata
    27181 tgtaaaaatc ttaattttgg aagatgatca tgccgggttc ccggcaaaaa gaatacgctg
    27241 agacgccaaa cggaacgatc catgcatagt tccgccaacg gcgtaaaaca aaaaataaaa
    27301 acaaaccatg aaaacaacaa ttcagaaaat tccattaatt cttgtaaccc tgatgtttac
    27361 ccttcgggtt tcagcagctg gagaagcccg tttgctgaga tttcccgata taaataacaa
    27421 cctcatcgcc tttgtttatg ccggcgatat atggaccgtt gattccaggg gcggagaggc
    27481 ccgccgcctt acatcccatg ccggaatgga actctttccc aaaatatccc ccgacggtaa
    27541 atggattgca ttctccggtg aatactccgg gacgaggcag gtatatgtta tcccctccga
    27601 aggaggatcg ccgcgtcagc tcacctggta caatccggtg ggcgtcatgc ctcccagggg
    27661 cgggtgggac aatgtggtac tggactggac tcccgacagc aggcagatac ttatcagggc
    27721 caacagaaca ccttttggcg acaggaacgg aaaatacttc cttgtcagcc tcgacggcgg
    27781 cctggaaaaa cccctccaga tagttaacgg aggattcggg gtgttttcac cagatgcgtc
    27841 gaaaatagtc ttcacccccg tcgaccgtga attccggaac tggaaaagat acaagggagg
    27901 cagggcaagt gacctctgga tatatgatct taaaacagac aaatcggaac agataactga
    27961 cttcgagggg acagaccagc ttccaacctg gtatggcgac aatatctttt tcgcttcgga
    28021 cagggacctt aaactgaaca tctggaagta caacacactg acccggcaga caacccaggt
    28081 gacaaaacac actgaattcg atgtaatgtg gccctctgga aacggtgatc agcttgttta
    28141 cgagaatgga ggattcattt acaggctgaa cctgaatacg ggccagacag aaaaggtatc
    28201 ggtaagcatc agttacgaca atccaaatct tgttccttat tacaggaacg tacaggagaa
    28261 tattcacagt tttgcagtgt cgccgacggg aaaacgtgtt cttttcgatg caagagggga
    28321 tattttctcg gttcctgcgg agaaagggac cacggagaat cttacaatga cccagggaat
    28381 aagagaggtt tacccggcct ggtcgcctga cggaaaatat atttcctatt attccgacca
    28441 gacaggggaa tatgagctgt atctgctcga aaacaagaaa gaagccaggc cccgtcagct
    28501 gacattcaat tcttcggcat ggaaatacca gcccgtatgg tcacccgaca gccgctacct
    28561 gctgttttcc gacaggacgc ttaagctgaa gctgattgaa gccgccagcg gaaagatcac
    28621 tgacattgac catgccaccg cgagtgaact gagaaattat aacttttcac ccgactcgcg
    28681 ctggataaca tatcagaagg aaggaccaaa cgataagcct gcagtgtggg tgtatgaaat
    28741 cccaacgggg cgcaggcagc aggtcaccag cggcattttc tccgatgaca gcccggtttt
    28801 cagcctttgc gggaactaca tatttttcgc ctccaaccgc gacttcaatc tttcattctc
    28861 cgattatgac tttgattaca tatacaacaa ggcaacccgg ttatatgcca ttccgcttac
    28921 agggaaaagc cccaggatat tcagggacaa aaatgacacc gaaccagtga aggagactga
    28981 aaaggcgggg acatcttcac cgaagccaaa aacagcaaaa ggcaagtcag ccgctgccgg
    29041 agccgatacc acggccggga aggaaatcag ggtcgatata gattttgacg gcatcaacga
    29101 aaggataacc ctgctgcccg gagaagcagg ggattaccgg atcgttggag cttccgaaga
    29161 cggccttgtt ttcatcaacg acggccgttt gatgaaattc aatttaaagg aagaaaagaa
    29221 tgaggagata cttgataagg tgatgacagc atctctaaca gctgacggaa aaatggcagt
    29281 ataccgttcc ggaaaggatt tcggaataat caggcttaca ccgggccaaa aggcaggcgc
    29341 cggaaagata aatcttgacg gacttgagat gaaaatcgat ccccgtaagg aatgggatca
    29401 gatctatgct gatgcctggc ggatctaccg tgattatttc tacgtcgaca atctgcacaa
    29461 cgttgactgg ccggcgatta aggaacgtta cagtgtcctg ctgccatatg tcggtcatcg
    29521 tgccgatctc gattatatac tgaacgaaat ggtggcagag agcaacaccg ggcactccta
    29581 tgttgactgg ggtgattttg agaaggtaaa gagaatagag aatggtctgc tcggggcaga
    29641 gcttactgct gatgaatcag ccggccgcta caggatctca agtatctaca gcggagagaa
    29701 ctggaacccg gcacgccgtt cgccccttac cgagcagggt gtcgatgtca gggaaggaga
    29761 ttacctgatt gcaattgacg gcagggaaat tacaacttcc gacaacccct atgcttttct
    29821 tgagaacaaa gcaggcaaga caattgagat aatggtcaac agcaaacctt cacctgcggg
    29881 agcccggacc tcaagcgtga aaccggtgac cagcgagctg gaactccttt acttcaactg
    29941 ggtgaatgaa agaagggcca tggtcgacag gctgtcaggg ggaaggatcg gatacattca
    30001 tgtacccaat acatcatttg agggaaacag ggaactgttc cacggcattt acacctattt
    30061 cgacaaggag gccctcataa ttgacgaacg ctacaacggg ggcggcttta ttcccgaccg
    30121 tatggccgat cttctcgatc gtcacaccct tgtctactgg caccagaacg gcctgacacc
    30181 aggcaagtct ccagcagtgg cacacgatgg accaaaggta atgctgatta acggatactc
    30241 atcatcggga ggcgatgcgt tcccctactt cttcaaaaaa ctcggactcg ggaagcttat
    30301 cggaacccgc acatggggag gactggtcgg gatctcgggc aatgcaagat ttgtagacgg
    30361 cggatacctt tcagtgccga gattcggcat cttcgacagc gacaagggct ggataatcga
    30421 gggaataggt gttaaccccg atatcgaggt gatggaccgc cccgaacaac ttgcaaaagg
    30481 cattgatcca tgcattgaaa aggcagtaga ggtcctgctt caggaattga aggacagacc
    30541 ggcgaaaaaa gtggttgccc cggctcctcc tgacaggtca aaatggcatg aataaaccag
    30601 caaagcctga gaagacatta taatcgctga taaccgccgc caaagccggc agttaatcgg
    30661 caagcgcatc caacaccgcc aaaaatgata atacagtatg catccgatct gcacctggaa
    30721 tttccggaga acagggaatt cctgaaaaag agatcgctta aaccaagggg tgatgtattg
    30781 atccttgcgg gagacattgt cccgttccgg ctgatgtacg atcattctga ttttttagac
    30841 tacctgtcgg cacactttga tataacatac tgggtaccag gaaaccatga gtattatcat
    30901 tccgatatat caaaaagaag cggctccgta aatgagaaaa taagggacaa tgtgttcatt
    30961 gtgaacaaca caacggtaaa cagggggaaa gtctgctttt tattctcaac tctctggacg
    31021 aagataggcc ccggccacca ggtggaattg cggaacaggt attctgattt ccgggtgata
    31081 aagagcggtg gaaggccgta cagcatcaat cattataatc tcatgcatga aaaatgcctt
    31141 tcattcctga gtgatgaact aaaaatccag agacgcggac cggtgattgt cgtttcgcat
    31201 catgtgccca ctttcagcaa ttatccggag gagtattacg gggatcccat gaatgaggca
    31261 tttgcagtgg agctgtccga tcttatcctg tccggccagc cggcatactg gatctacggc
    31321 catctgcact caaacaaggg agagttcaat atcggcaaaa ccatcctggc aacaaatcag
    31381 ctgggttatg tttcatacca tgagcaggaa ggatttgacc ccgggaaaat tattgtaatt
    31441 tagccggccc gctaaaggag cataaaatga actttccact gaaagccatc cgctaccgga
    31501 cttttattga tcccctgctg gccggtctca ggaatgaggt gcttctcaaa ataaacagcc
    31561 gcgaccgggt tcttgagatc gcctgcggaa ccgggatgct tgccatggca atggcgtcaa
    31621 aagcatcaca tgtaacggca acggaccttt cggaggaaaa tattgccgct gcttcgcaga
    31681 gtgccgggaa aaacgggatt gaaaaccttg atttcggcgt gctcgacgct tccgatcttt
    31741 catgctattc gggccgggaa ttcgacgccg cagtgatatc tatggcaatt catcagtttg
    31801 atcaggagct cgctgtaaaa atacttttgg aactgaaaaa tatcgcctcc aggataataa
    31861 ttgccgatta ctcccatcat atgccccgcg gctggggaag gaagcttgcc actgcaatag
    31921 aatggctggc cgggggaaat cattacagga atttccgtaa ctacatggct gctggcggaa
    31981 ttccgtattt cgcagcaaag gccggcatta aaccagtaag cagatcagta cggggacgag
    32041 ggatatttct tgttaccgtg tgtaactgat ctgtcagatg taaatatcac gttttatgcg
    32101 aggcttccgg gcaaaagcag tgattcccgg ataaccggtg caaaccttgg ttaagggaga
    32161 catattccat tgccccggcc tgttttgatg atttatggtt accccggatc cggagcgtga
    32221 gctctgaaag agcggatata tctataaccc cggctggcgc ccggggctat tcacatagcg
    32281 ccgcattcgt ggcttaagct gcggcctgat ctgtccggcc tggccatcac gcctgtattt
    32341 agcattctgc tatttcgatt tagaaggaaa tcttccagtg tcaaagcctt tatataggac
    32401 ttatggtttc cgggaccact gaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32521 nnnnccggca ccagccgggg gattagtgac cccactgcca atcagctccg aaggagcgtg
    32581 acatcaatat gagtatgtct caccgggggt atctttactt ttattgttga tgtgactttc
    32641 ttttgcatct ccaaaagaaa gtcaccaaag aaaaagagcc cggaaaagac aaccgctcct
    32701 gtttttcgcc gtttgcgcga gggccgtttc ccgctccaaa aaacaggtgc ggttcgcacc
    32761 ttttccggtc tgcctccgca ctgccggatt cagcttgctg ctatgattat tttgaagggt
    32821 atcttccagt gtcaaagcct ttatatagga cttatggttt ccgggaccac tgaaagcaag
    32881 ctctggaaga gctatatatt aataaccccc ggcaccagcc gggggattag tgaccccact
    32941 gccaatcagc tccgaaggag cgttacatat atttcaattg cagcatagtt tgtcggtgaa
    33001 gcaacctggt ttttgtacgg gaaatccttg cttttttatt gtcactttct tttgaccctc
    33061 caaaagaaag tcaccaaaga gaatcataaa aatatgtgaa aggtgaaatc ctatgcattg
    33121 caaagcgtta aggaagatat tccttatcac cgttttgctt atttgaattg agaatgagtc
    33181 tgcggaataa aagttacttt aaaaaagagg gttagaaacg taaaattccg taaattgaga
    33241 gtagcttttt aatatttcag ttgaggatac tgtaatgaaa gccattggat tttttatatc
    33301 agctgttctc ttttttagtt tggcacatac aggctgtaaa aaagataaaa aggaatatca
    33361 ggaatacata agctataccc tgacggttaa agttgataat ggtgctgatg ggggtccaaa
    33421 atcagggtca tatcaaaatc cggccggaag catagtcaat tatggttaca gagtattgga
    33481 tgactatact gacatggttg tcatgcttgg tactgatact gttccttatg aaggcagttt
    33541 cataatggat ggaaataaaa cgctgacggt gaccaccaaa cagatggtgc tgtggaaata
    33601 tcagacagaa aaggcggtat attattcaac tcctgcaatc ggagatgatg gaacgcttta
    33661 ttttggcacc gggatgggtg gaacaagtga cggagccttt tttgccattt caggagatgg
    33721 aacactgaaa tggagctttg caggtcaata ttcttttttt tcaccggtaa ttgatgcatc
    33781 aggacacatt tatgttcagg atttcggata taattgctat gcattttatc ctgacggtaa
    33841 attgaaatgg aaattaccaa atactctttt cccgcgtatg gtggaaaatg ttggccagcg
    33901 ctgtcctgcc atttccgcag acggtacgat ttatatatgt gctgacggcc tttatgcggt
    33961 ttctcctact accagggaaa tcctgtggca tttccccgag tcgcggacca agtcgtcgcc
    34021 tgcaatcggg cctgataaaa ccatttatgt tgtgtttcgt caggacaggg taaaggcaat
    34081 taatcctgac ggatcactaa aatgggaaaa caggtttgac aacccgtggg aaatgtcgta
    34141 tgctgctcct gctgttgacg accgtggttt tgtctatatt gcatctgaag cgttttacga
    34201 agggaaggat tattctgcct tgtatgcttt ccgtcaggat aatggggaaa ttcagtggag
    34261 atatcccgta ggcctgtttg taagagcttc acccgtaatc ggcacaaacc gttccgtata
    34321 tattgctacc aaagcaacag aaacagatat agccagacta atttgtctct cccctgaagg
    34381 agagaaaaga tgggaatttg aagtggaaaa tgttcatggt atgcctgatg atatatattc
    34441 aactccgacc ctcggggctg atggcctgat ctatttcggt gcagaaaccg gatttcttta
    34501 tgctgttcgt gcctcagacg gtgtcttggc ctggaaattt gatctgaaaa tgacggttaa
    34561 ctggtcatca cctgttattc tgaaaaacgg caccctttat atcgggggaa tgggaggtac
    34621 agattatgaa ggttatatga aggcgataaa aaccggctgt ccgggttatg ctccctcacc
    34681 atggccaagg ttcaggcatg ataataaaaa cagcggagct tcagattagt tattgcaata
    34741 ttttcaaagt tgtgaattgt tttcaaaaaa gagttttgac ctaaataaag tgatttaaca
    34801 aaaaaaaagg taacaaacgt caaaatttcc agacgcgggg gcttgccaac ttcttacgat
    34861 atgccgctga aacgcttctg attgccctta tatcaaacaa actgtatcgc agtattttac
    34921 acaaactttt cgcctggcgc ctgaagacag agaaatccaa tattgttttt tcaggcatca
    34981 acaccatgga tatggacaac gacgacgcta agaaacgggg ggttgtgagc cgacctaaag
    35041 agaaagaaag ggtttcaacc tttgcatgtc ttttggagca ctttcctgat cgatgttatt
    35101 ctccggattg ccttcgcgcc gccggattca gattgctgca atcctgacat gcattgcctg
    35161 aatgtaagac tatcgaaggt atattaagga atatgcctca aaaaggagat cctagctttt
    35221 tggggattat tttaaacgcg ggctcagaat tgataaccca tgcggaatcc tgctgtaaag
    35281 ttaaagagag aacgtattcc ggcttcgcta tttatactgt acataattac ttcataattg
    35341 ggatctatag aatgtactat ttcatccatt gatggattga attgcaggtt atggttttca
    35401 gttttcctcc gacggagtcc gaagccgcca taactttcaa ttacaatatg ttttataaat
    35461 acctgttgcc agccaaaaac caaatttgct ccccacacat gctttagaac tgaaaagcta
    35521 tccggaatac ctatatcggg atcactatct ttggtgtagt ataataaatc attgaatgaa
    35581 tgcctgatgc agaaagtgtt tattcccaga aataaacccc gttcattggt tcctttctga
    35641 ttattccagt tcttaacgta atacctgatc tctgttttca gtttatatcc ctttgatttt
    35701 acaaaagatg tatctgaata ttcataagat gttttcataa atttaattcc agcctcagaa
    35761 tataatgcca accttttagt taaccttaat tcaaaaccat gctggatggt aggaaaagaa
    35821 taatcgtcga agatacataa cggtgaaatc aaacctttta tcttaatatc ctgggaatgt
    35881 gtatataaag atagaattga aaacaatgga aaaagaagtt tcttcatctc ggctgttcct
    35941 tagttaattt ctttgtttca tccgggtatc gaggattttg ctatgaattt atgtatttat
    36001 ttttgcatgg tttaaagatc atcaggaata ataagtatca gatagtttta ttttttttcg
    36061 tatgaaatat attatcaggt atttaacgaa gaccgggcct gaaagcttgt ctgtttgcgg
    36121 cttcatgatc aattatcatt gaatcaattc attgtgaata tattaacaca tcagaacagc
    36181 tttcttataa gccacagcag cagggacaaa atcacactga ccactatcat tgtggtaact
    36241 gggaaataaa acctgatatt ctccctttca atcctgatat cgccgggcag attgcccagc
    36301 cagttcagtt tatctcccag gaaccagatt atcagtcctg caatgaccag gaccacccct
    36361 gcaattacca gaattttgcc tgtttggatc atacgctata aatcccggca cttaaaccaa
    36421 tgaaaaacct gccggcggca ttgaattatt ttaaactacc accccaaatt tagaatttaa
    36481 atttaaatta gtatttctgc tcttcactga aaggacaacc ggaagatatc catttagtct
    36541 gaaaactgaa tccttgccgg ccctgtcaga aaagcaggtc cggatgctgt ttaatgaact
    36601 gattggctgc cattatgcct gcattccgaa aaacgatcaa caaccaaatg acaacaagat
    36661 gaaaaacctt atcacttcac tgcttgcttt tgcactcatg gcgggattga atgcccagaa
    36721 caccgacagc atatggatag tgaacaatta caccaagatc gagaggatga taccgatgcg
    36781 cgacggagtg aagctgttca cttctatata tattcccagg gacatatccg aagatcatcc
    36841 ggtacttatg gagaggtcgc cctattcgtg tgcaccgtac ggagagggtg catggaggga
    36901 tttctggaac aggtatctga aatattacct ttttgagggt tacatcatgg ttaagcagga
    36961 tgtccgcgga cgctggatga gtgaagggga gtttgccgat atcaggccgt tcaatcctca
    37021 caggaaggac aacagcgaca ttgatgagtc gagcgacaca tacgatacaa tagaatggct
    37081 tttaaagaat attcctcaca ataacgggaa ggtgggtgtt ttcggaattt catatccggg
    37141 tttctattcc acgatggctg cagccagtaa tcatccggca cttaaggccg tcagtccgca
    37201 ggcgcctgtg acgaactggt tcatcggcga tgattttcac cacaacggag cttttttcct
    37261 gctcgatgca ttcaggtttt atccggccct gggagccggt tttggcctgc cccgtccgaa
    37321 accttcaaca aaagggccgg aaaacatccg tctgccgtat caggataatt acaggttttt
    37381 ccttgaaacg ggtgcattgc ctgcttttgc caggctggca ggagacagcg tacctttctg
    37441 gaaggagctt tattcacatc ccgattatga tgagtggtgg aaagtccgcg acgcaaggaa
    37501 cgccacaaaa aatctcaacc ctgcaatgct gtgggtcggc ggacattttg acgcggaaga
    37561 caactggggt gcctggaact cttacaaggc agctgaagag aataatccca tgaaggaatt
    37621 caacaggatc gtaatgggcc cctgggcaca cgggcaatgg tcgtggcctg acggctcata
    37681 tctggggaac atcggtttcg ggagcaatac ctcccagtgg taccagcaga atattgaaat
    37741 accctttttc aattattatc tcaaaggtaa gggatcgaag cctgaaattg ctgaagccac
    37801 catcttcata accggcagga atgagtggaa ggaattcggc agctggccgc ccgccggaac
    37861 tgtcaacacc gacctgttcc tgcagccgtc agggaggctc ggctggcagc ggcccggatc
    37921 aggaccagag cacagtgatt atgtcagcga tccgtcaaag cctgtgccct ataccgagga
    37981 cgtccatttt ttacggacaa tggaatatat gacggatgac cagcgctttg cttcaagaag
    38041 gcccgatgtt cttgtgttta agacggatat cctggaagag gagatcacgc ttaccggaat
    38101 gctgaccgcc gatctttttg tttccctctc atcaactgat gctgactttg ttgtgaaact
    38161 gattgacgtt ttccctgacg atccgggaca tctcaggaca gagccctatt ccgtaagaat
    38221 gcccttttcc aaatatcctg gcggaggcta cgaaatgctc gttcacggag agatattcag
    38281 gggccgctac cgcaacagct ttgaaaaacc tgctcctttt gtacccggaa aagtggagag
    38341 agtgaaattt gaagtgggca acgttgccca taccttcaag aaggggcaca ggataatgat
    38401 ccagatccag agcagctggt tcccccttgc tgacaggaat ccacagaagt ttgtaaatat
    38461 ttatgaagca agggaatcag attttcagaa agcaaccata caaatttatc acagtaaaaa
    38521 gtacccggga aagataatat tgcccgttct gacacaatga tcagtcggca gatcaacagt
    38581 gttcccttgt ccattctgga ggaggcaccg gtttacggcg acgggaacaa ttctcgagga
    38641 aatatcagga gatgctgata tacagaatta tagatatctt ttttgttttg ttccatacct
    38701 gtcttataat cttcaatctg tcaggctggt tatggaagag aaccaggaag cttaacctgg
    38761 tgtttctgct tctgaccgga ggttcgtggc tgttccttgg gatgctgacg ggtacccttg
    38821 gatattgtcc cctcaccgac tggcatttca gggttcttga aaagcttggg tacactaacc
    38881 tgccggcttc atatatcaag tatctctgcg accggctgac cgggatagat ttcaatgcca
    38941 ttttgattgg ccggctgacg ttttatcttt tcctggtcgc attgatatta tcccttctcc
    39001 ttaacctgag agatttccga aaaagtccac cggaaaagtt agccgcttcc ggcaaggata
    39061 attgctgacc atgcggcaaa ctggcaggga aatcacagga ccaggaaaga tcggcagctc
    39121 cgttaaatgg cctcaagtgt tgaaggcggc tttgaagcta tgttataggt aacggacaca
    39181 acctccggta cattctcaag gatcgaggaa gccagttctt ccagggtttc aaacggcagt
    39241 tttgtaggac ttgctgttct ggcgtcgacg ctgtcccagc aacgtatttc tatctggtgt
    39301 ccgaacttac gttttccttc cctcattccc gtaacccggt cgttgtggag gatggccata
    39361 tactggaagg cacctgtacc ggcaaggatc ctttcaacga cagaagtggc cttttttaca
    39421 agatcgagct tttcagcggt aacttccccg atgatccgtg ccgccagagc cggtccgggg
    39481 aaaggtatcc tgttgaacat ctcttccggc agtccgaggg cttttcctgc aacccgcacc
    39541 ccatcctttc gcagccggat caatggttca aggatctggt atccgaaagt ttcttccgga
    39601 tcgatcccga gctgtgcaaa aacattgtgc tgtcttttta tcccggccac tgtttcgtca
    39661 atatccgtaa ggatggtccc ctggagaagg aattttgcac cgctttccct gacgatgcgt
    39721 ccaaaaacct ttttgtagaa ggtctgggta atggcttcac gtttttcctc agggtcagtt
    39781 atgcctttaa gggcatcaag aaactctttc cgggcatcaa ctatctcaac tttcactccc
    39841 agtttgctga atatgtctgc cacaaattca gcttcgccct cacgcataag tccgttctgg
    39901 acaaaaaccg tccgaagcct gtggcccagg gccctgtggc ccagcaatgt cacaactgaa
    39961 gaatcaactc cccccgagag ggcatttata gcggtgccct gtcctactgc agaaacaatt
    40021 tcatttaccc tttcagttat gaaattttca gcgttcagat cgtgaatttt aatttcgttt
    40081 gtcattgtct taaatatatt ccgtaaactt ataaaatttc cgctttcaca tttttcccgc
    40141 cacacaatta attgagataa tgttgaagtt ggaaactctc cccgatcaga taaccctctg
    40201 gccatgacat gtaagagtga cgatattgat cccgggaact tgctcccgta acataactct
    40261 ctggctatga catgcaggaa cagggcgata ttaataccgg taactccctc ccataacata
    40321 actctctggc agagacatgc tccgaaggag catccgtcaa ccaaagcctt ctcagaatga
    40381 gaagaataac aataacctcc ggtgaaaccg gaggtacggt ccacccctga ctgttcagct
    40441 ccgcaggagc tggacaatac caatgagggt gactcatctc cccccggccg atgtttttgt
    40501 taataataca acgagatgta agcaagatac taaattccag ggaaaatccg ggtttgtgtc
    40561 gccccgttcg gggc
//
LOCUS       DAOOYG010000011        40291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_828_length_40291_cov_7.031763, whole genome shotgun sequence.
ACCESSION   DAOOYG010000011 DAOOYG010000000
VERSION     DAOOYG010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 40291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40291
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_828_length_40291_cov_7.031763"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3100)
                     /locus_tag="PK288_02075"
     CDS             complement(<1..3100)
                     /locus_tag="PK288_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HPM17732.1"
                     /translation="MSWIKDMISPETRQWEEFYRNRWQYDKKVRSTHGVNCTGGCGWH
                     IHVKEGIVVWEFQALDYPMLENSLPPYEPRGCQRGISYSWYLYSPIRVKYPMIRGALI
                     DLFNEEKKMTGDALLAWQNIQNDPVKRREYQKARGKGGFRRATWEEVLEVIGAANIYT
                     AKKYGADRVVGFSPIPAMSMISFAAGSRFLQLFGGVHLSFYDWYCDLPSSFPEIWGEQ
                     TDVGESADWYNAKFIAVMGANLGMTRTPDVHFFSEARHNGTKTVVFSPDFSMVSKYAD
                     QWIPVHAGRDGAFWMAVTHVILKETHSDKQVPYFIDYTRKYTDAPFLVEIIEEGGRMV
                     PGRLLRANQLEKYADIENGDWKFLNIDEGTGEFICPSGSSGHRWQDKKGAWNLKYRDG
                     ETGTEYTPLLTLIEKSDKILQVEFTDFARDTKSKRGVPVRYMQTRRGKIAFTTVYDLI
                     MGQYGVDRGLGGDYPSSYDDAGSAYTPAWQEIFTGISSATVIQFAREWCLTAENTQGK
                     CMIIIGAGINHWFHSNLIYRAATMALMFTGCVGVNGGGMNHYVGQEKLAPFDSWGSIM
                     SGSDWNIPSRFQQSPIWHYIHSDQWRYDGNQADYNSVPKNEFSSRHSADMIVKAVRNG
                     WMPFYPQYDRNNFEIAAGAREAGALDDKAIQDYVIDQLRKGELNYSVADPDNEVNFPR
                     VWYIWRGNALMTSAKGHEYFLKHYLGTHHYEIADQVAKEFVKEIEWREDAPEGKMDLV
                     VDLNFRMDTSALYSDIVLPAASWYEKADLNTTDMHSFINPLAPAVPPVWEAKSDWAIF
                     REIARVTSETARKHFPEPVLDIVNRPIGHDTAGEISQPVIKDWFTGECEPVPGKSMHN
                     IITVERDYTRIYEKFISLGRNARSRLGVHNISFRCDDFYDRMLNDKNHVNRVGDQIYP
                     SIGEDTEAINAILLLSSVTNGTLSGRAFSDIEQKTGIDLTPINPKNNDITLRFDDLQA
                     QPRRLLNSPIWSGLMTNGRAYSGYTLNIEHLIPWRTLTGRQHIYLDHEGYIHFGEHLP
                     TFK"
     gene            complement(3160..3963)
                     /locus_tag="PK288_02080"
     CDS             complement(3160..3963)
                     /locus_tag="PK288_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPM17733.1"
                     /translation="MKKISLFAICLFLFPANHLLQAQSDGKEIFESTCTACHTIGSGR
                     LVGPDLSGIYQIRTNEWLISFIRSSQQFIKSGDSLAVAIFEEYNRVQMPDNRLSDAQI
                     LSIIDFIRESDQDLQAVGGAAAISEDSLAIIYSIEHMPAGRALFYGHTRFANNAPPCI
                     SCHNINDQSVLGGGKMALDLTGSYAKLGAPGIAAIIKNPPFPVMKRAMLNHEINEDES
                     QALISLFKAAGEQPYPAGKRGLIFFGLGLVCALILLGHIYILYDRRRIP"
     gene            complement(4190..4909)
                     /locus_tag="PK288_02085"
     CDS             complement(4190..4909)
                     /locus_tag="PK288_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPM17734.1"
                     /translation="MAKTEPFDQYPEEYEEWFSQNHYAYLSELAAVKKALPDKGRGVE
                     IGIGSGKFALPLGIKEGVEPSKAMREKARERGLSVTDGVAENLPLPDSSYDFVLMVTT
                     ICFVDDLNKALAESYRILKDDGCIILGFVDRESPVGKLYLTIKEQNVFYKDAVFYSTG
                     ELYRYLADSNFEVMETFQTIFGLPEEIRVVQKAEKGYGTGSFVVIKARKISDGNWRTP
                     GSKHHPEAGRDRISSETGHSR"
     gene            5109..5702
                     /locus_tag="PK288_02090"
     CDS             5109..5702
                     /locus_tag="PK288_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008659750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HPM17735.1"
                     /translation="MRIKTCIYLLAGVLLSGLISMNSCNQSRPEKVRVIGQKIDSLNG
                     VYVFYNGAVGNVSGRNTTRDGYNLGLKYQCVEFVKRYYYEYLDHKMPDSYGHAKDFYD
                     KTLGDGQKNKRRNLIQYSNPGRTKPAVRDLVVFDGTAFNKYGHVAIVSNVTKRKVEII
                     QQNPGSNGNSRESFPLREKGGKWEIGDKLILGWLRKE"
     gene            6014..6988
                     /locus_tag="PK288_02095"
     CDS             6014..6988
                     /locus_tag="PK288_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17736.1"
                     /translation="MKPMTLFVLAFALIFSGISAQQVVWQPDVIKRLTPEWKGERYPD
                     GRPKVPDALLERLKNCSFEEIQAYLGMHGYRNVFENFASLNENGWHIIHPDKVMTGRA
                     LTAQFMPLRPDFNNYVQAQAKEEGTHTPVTNYAPIIKLQEGDIYVADSYGKMEGGTLI
                     GSNLGNAIANASKRGVIFNGSLRDYEGLEAIGENFNGWIRGYDPSGIQQMMTAWVNAP
                     IRIGRVTILPGDAILAKKLGIVAIPPHLLLGSVLHSEYITLRDEFSFLCIKTKRFPYI
                     NEAFVVDHDVYEKAFNEWLDTQNLPMTRNELNSYLKEMEELRASSRKK"
     gene            7317..8846
                     /locus_tag="PK288_02100"
     CDS             7317..8846
                     /locus_tag="PK288_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM17737.1"
                     /translation="MKKLLSAALILVLWLLAGPGIKAQGTSLEEELIYLLGSKDRRHA
                     AMLARDVRYVDYTDLHDRSLLMYASANGYGGICRILLKKGADPGNQALDGVTAAMYAA
                     YNGHKRIIRLLLKEGINPDTQAIDGLTALMMATQNGYRPIVKLLLDAGANPNLLTNDG
                     YSSLMLAAQYGYDEIARMLIDKGADVNFRNINNVTALNQASLSGSAEIAKMLLKRGAD
                     PDSRNNEGISALMTASLIGHYEFVKTLVESGADVNLKNKDGVSALFLASLNGHTEIVD
                     FLLARGAKPDTRDTDGTVALMINAREDIAEALIQKIYEGTKDVSKSGDHMIVLLSKTE
                     NGDLEAVKLFMDRRANAAMLASSNKAAAFIHSSGNGHIAIVNLFIKKDARVETNKTAR
                     FTDLMAASLNGHSEIVKLLTEKGAKPNLTGNDGATALILASQNGNAEIVKLLLEKGSD
                     INLQRSDGFSALMAAVKDGHADVAKLLLEKGADPRLKDIMGKTVVDYAEDAEIRKLFV
                     K"
     gene            8912..9802
                     /locus_tag="PK288_02105"
     CDS             8912..9802
                     /locus_tag="PK288_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HPM17738.1"
                     /translation="MKTIRAYTFLILLTGILVIYSCDRKPYILKDGYIYVQKEDRRNE
                     SAEWQLVWEDEFDRGELDTTIWSRIGLFETPKWKVPVEKWREVTNCFRYITATDPRVV
                     QFDEGNIHLRGIVNPDSLSGDPRPYLTGGIYSWRKFAFQYGRIEIRARLDQAYGMWPA
                     IWMLSEKEIYPDQHNGEMDIMEKLNHDDFAYQTTHNHYTIALKQNEPKKHATGKIDTT
                     DYNVYSVSWYPDKLVYAINGVVSYEYPKVPGAGTYQWPFDQPFYLLIDQQMEHEWPGK
                     ITNPGELPISMTVDWVRLYQ"
     gene            complement(9839..10843)
                     /locus_tag="PK288_02110"
     CDS             complement(9839..10843)
                     /locus_tag="PK288_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPM17739.1"
                     /translation="MKSNCFTALMAGLFLLLADIFNPDLVYSERPVTVATIGNTPALT
                     DNDNLQATVDYVISFWEKELKKVLKARPDLIVLPEFADLSIAGVEYMKVRGNQVLDFF
                     SSVARENKCYIAFGMGRQDSMGLWRNSCMVLNRKGAIAGIYDKNYPTISEIQTGIRAS
                     DDIAVIDCDFGKVGVAICFDLNFDELLAKYSSTETDIIIYPSMSHGGVLQNLWALSCN
                     SYFIGSIYRDFPSEVRNPLGKVIATTSKAADYAIANINLDYKTVSLANNLEKLVALKS
                     KYGNTVQISETSRNGRVIVTSSNKSVSSEQMIREFNIEPFNEYLDRARSIRTEDMPAD
                     "
     gene            11576..12706
                     /locus_tag="PK288_02115"
     CDS             11576..12706
                     /locus_tag="PK288_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPM17740.1"
                     /translation="MKKQLILTLILVMSGSLNAFVFPQASPDEVFTVKGFSCTSPRRH
                     EVDRFVQFIDRELAPRGVNTLVLFVCDKDFNYQFESHPELSDPSMLSKEDVKKIVASC
                     RKNKIRVIPQINLLGHQSWREKPNILLEKYPQFDETPHIRLPEKYVWPNDDDLYCKSY
                     CPLHPDLHKVIFALVDEICDVFETDAFHAGMDEVFYLGDDKCPRCSGVDKAELFAGEV
                     NRIRDHLAKKNRELWIWGDRLIDGKTTGIGLWEGSYNYTWRAVDMIPKDVVICDWHYE
                     RADHTPVYFAMKGFRVITCTGNKPETSVAQAEDIVRFRTNTTSEMKPRYYGIMQTVWS
                     STDQFIDAFYGKQPAGPAGRRNPADGFKALYTRINELEQKKP"
     gene            13113..13913
                     /gene="larE"
                     /locus_tag="PK288_02120"
     CDS             13113..13913
                     /gene="larE"
                     /locus_tag="PK288_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPM17741.1"
                     /translation="MGTQDKLDILKHKLGEYKSAVIAFSGGVDSTFLARVAKDVYGDR
                     LMLITASSSTYPFYELEEARSLASMMNIKHKVIVSEEIDIPGYSDNPPDRCYYCKSEL
                     FGKISFIARQEGYEVIFDGSNAEDLKDFRPGLRAKNEKGVISPLAECGFTKAEIRYYS
                     EALNLPTANKQSYACLASRFPYGEKITIEKLDRLSVAELEFRNLGFSQFRIRSHDNMA
                     RLEFRPEEMDRAWECRDVLTKICRNAGFVYVAIDLTGYRTGSMNEALT"
     gene            complement(13995..14690)
                     /locus_tag="PK288_02125"
     CDS             complement(13995..14690)
                     /locus_tag="PK288_02125"
                     /inference="COORDINATES: protein motif:HMM:NF039204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HPM17742.1"
                     /translation="MKIGRVRLRKEYRRKVWSFFITILLLCAIFLPYSNRALILNFFS
                     SKCRNYQQVYSKKLNDRIVDYSDKARAKGIEKCSDAVDLRNRVSARQLRKVSSGRYYR
                     IENMTHSFPYLTSDSKRLLDEIGKRFRKKTEKEGFPGSRFVITSMTRTSENIAALGKA
                     NINASADSPHLYGNAFDISYARFLFRKYKVTDCDKWYMKEALAEIIYELKQENKCWAT
                     YERQQGCFHVVSR"
     gene            15051..16391
                     /gene="mgtE"
                     /locus_tag="PK288_02130"
     CDS             15051..16391
                     /gene="mgtE"
                     /locus_tag="PK288_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPM17743.1"
                     /translation="MVELIDEFGELLENKEWKTLRKRLSEYEDLDIAGLIEELPDEDD
                     IVVFRLLSKKQAKNSFQLLSHDKQEQIIEGLARNTTKLSALLNDLDPDDRTAFFEELP
                     GEVSQKLIQLLSPEERKIAVSLLGYPEESIGRLMTPEFVAVKSHFTVGQTLDHIRIFG
                     HDSETLNVVYVVDDKWKLIDDIKIREIILADPDQKIMDLLDSRYVSLNAYDDQESAIR
                     VFKDHDRVALPVTDSEGILLGIVTFDDIMDIEEEEATEDFHKFGSFQDVIIDPLKARI
                     PFLYQKRIVWLTILVFMNIFSGAAIAGFENVIQSTVSLMFFLPLLIASGGNAGSQSAT
                     LMIRSLAVGDVEMKHWFRLVGKEFIVSFLLGITMAVGVSLIASVRAPEIILVVALTMI
                     LAVMAGSLIGLTIPFVFTKLKLDPATASAPLITSIADIVGVWIYFSIAAWVFGL"
     gene            16570..16989
                     /locus_tag="PK288_02135"
     CDS             16570..16989
                     /locus_tag="PK288_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17744.1"
                     /translation="MKTAKKILMLLITISVTSFLLNGQAVRVHQDTAVMVRQEGQTQE
                     QTGAAERPGRQNQTWSQNHAGQDGRAGNRTKVSAGNSGGSQPREANAGEVKKIRGARP
                     DWSRARGARPASVERPSGSRIPKGAGKPGGAKGPGRR"
     gene            17001..17912
                     /locus_tag="PK288_02140"
     CDS             17001..17912
                     /locus_tag="PK288_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17745.1"
                     /translation="MNVMIRNRIRLYTFAGSLVLQTLAVYAQTQPPSAPPAYKNATDT
                     AAQNRMHSFYAGTGGGNNMLYLGSTMSQGHGFGFASVSYGLMDKIYFSATGYTVNKLS
                     PFTAFYSLGLSFNHTFNSWFDISAGFSRYNVSAPLRDTLFDNFSYADVTLGFDWKILY
                     SKISAGSLFSGDKQFYLQTRHSRYFETPSFAGGKAFFSFDPYINILSGTVVSIETSGD
                     VKETTVSPGFRPWRKKRQGSNPSPVTTYNEYFGLMEIDFGFPAAFSYDFFTIELEPGY
                     VIPLYSLEGSSGTGGFLFMASLFLKIF"
     gene            17922..18602
                     /locus_tag="PK288_02145"
     CDS             17922..18602
                     /locus_tag="PK288_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM17746.1"
                     /translation="MNVLIVEDEKSLANEIAIFLKRENFLCDMAYNGAEASEKIAVNL
                     YDFVLLDLGLPDYDGLDLIREAKKNNADVSFIIITARGAVEDRIKGLDLGADDYLPKP
                     FALPELHARIMAVARRKFSIPSPDITLGEFVMIMAGRKLMHDNHEVEMTKKEFDLLFY
                     LAINRNKVLTRHQLYEHIWGNMLDDQYDSNFIDVHIKNIRKKLNVRAPAPWLETVRGV
                     GYRISTDY"
     gene            18617..19939
                     /locus_tag="PK288_02150"
     CDS             18617..19939
                     /locus_tag="PK288_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM17747.1"
                     /translation="MMKLRTKLALFNLLSKVVFSALFIGFLPFIIERINIRQTDNELV
                     NKREEVVSLISDTGIEPFIVSDTAGGFGSFNILKEEYISLERVDTCEELNFIDVTKRM
                     IDEEIIDYRVIHYSLRIDGECYLLEIGKSLSSISKTGRNIRNIILAFLVLFILVSFIS
                     DILYTARILRPLRIITGRLGKISSHSFDPSGPLITTTDDFARLDRTLREMMKKIDELF
                     RKEKEITVNISHELMTPVSVIRGKLENILLRNNLDPSVEAGIEETLRILYRLKTLVNS
                     MLMIARMESSQYLKEENVELCELIREVTGELSPLAEDGGISISLQCEGTGLFGKVNRS
                     LLFSMLFNVLNNAVKHTPRGGSIVIMAGDNAGRFEVSICDTGRGMSREQLDKLFTRFS
                     NTSGQATESMGLGLAITKSIADFHNIDIKVISEPGKGTQFFFSFPVNS"
     gene            20005..20490
                     /locus_tag="PK288_02155"
     CDS             20005..20490
                     /locus_tag="PK288_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17748.1"
                     /translation="MRKKILFGVLALLISAGVTSQTTEPLQEQTKTKKQLRTETKQQT
                     QIRENIRSDDPDMTRTRTREQIKLQDQSGDPIMTKDQIRLRSKRQLRDQSCDPAMTQQ
                     ARARTSNHGLTVSKTARQTPGGKGKGGIVSAQARQVHRNMSAARGARTGQPIVRNAGR
                     R"
     gene            complement(20566..21741)
                     /locus_tag="PK288_02160"
     CDS             complement(20566..21741)
                     /locus_tag="PK288_02160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 domain-containing metallophosphoesterase
                     family protein"
                     /protein_id="HPM17749.1"
                     /translation="MKRSLLFLPVLLSVSLLAYGQELPFRITHGPWLCDMTETGVTIV
                     WMTNKNSLSWVEVAPDDGSHFYGKERPKYYDTHMGRKQAATNLHKVRIENLSPGTRYR
                     YAVFSKEVLHWENDAKIIFGTTIANPAYSWNSLSFKTFSANDDTVSFIMLNDIHGRAE
                     FMKDLCRDIDFSSIDLVIFNGDMSTWIISEEQIFSDFLDAAADLFAKRVPIVFTRGNH
                     ETRGPYADFLMNYFPVKDGKIYRIVHVGKVAFIILDCGEDKPDSDIEYSGLAAFDAYR
                     DEQAEWLKQAVQHKSFRDAGNRIVVLHMPPGVSNWHGTLHLDETLLPVLNEARIDAVL
                     SGHMHRYSYNPPVPGKTAFPVIVNSNNTYLRGDIINGTIRIRMFGAEGGKPTEHILD"
     gene            complement(21872..22717)
                     /locus_tag="PK288_02165"
     CDS             complement(21872..22717)
                     /locus_tag="PK288_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPM17750.1"
                     /translation="MNKYYNSPGTLRPSDRFRSFCHAFLAMLLFLSFNLPLPAQQIRV
                     MTYNIYHGEQHFNPGKSNLAMVAEVINKYKPDFVALQEVDSMTMRSAKLNNDIPMDLV
                     AELARMTGMHGFFGKAIDYSNGGYGEGLLTRFPAESSNYSLPNPKGGEGRVLLLVSHT
                     FPNGQKIIFAGTHLCHQYAENRLAQAKAVREILEGTKLPVIVGGDFNFTPDSEPYKVI
                     NGGFRDAALIKGNPQITYPPEILRIDFIFLSGGHDWTVRDVEVIPASASDHRPVLVTL
                     ELKTR"
     gene            complement(22943..23839)
                     /locus_tag="PK288_02170"
     CDS             complement(22943..23839)
                     /locus_tag="PK288_02170"
                     /inference="COORDINATES: protein motif:HMM:NF040125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6528 family protein"
                     /protein_id="HPM17751.1"
                     /translation="MRTYMRLAVVTIILIMTGYSCKHRDSQEIIACGDDKVLIIDLDN
                     STGENVKVVWEWTVGDAADLSSGMKKLMVPTDDCKPVDGGKKILVTSSGGGVVLIDRQ
                     TKKILFSAKVPMAHSAEYLPGGLIAVALSTHEDGNSLELYDIKKSDIVLFRDSLYSGH
                     GVVWIPAIQRLFALGFNELREYSLVSDDIGSPGLQLMREWRTPDDGGHDLTAVSASEL
                     LVTTSKSVWRFDIGKEKFTAFEMLEDGADIKSVNLNPATGQLIYTKAEISWWTHHIYC
                     LNPDKVITIPDIDLYKVRTFNR"
     gene            24267..25418
                     /locus_tag="PK288_02175"
     CDS             24267..25418
                     /locus_tag="PK288_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPM17752.1"
                     /translation="MSRKRTVEEINEKIRKGKAVVLTAEQVAEMGRETGSAGVAEKVD
                     VVTTGTFGPMCSSGMFINFGHTDPPIRMEKVTLNNVPVFAGIAAVDAYIGATETALPH
                     DQYGGAHVIEELIAGKDVLLQATAKGTDCYPRTEVKTLISRDSVNEMIMFNPRNAYQN
                     YNAAVNSTLKMKYTYMGTLLPSFGNATFSTSGELSPLLNDPEMRTIGIGTRIFLGGAT
                     GYVAWHGTQFNTGKPLNEHGVPVGNAATLALIGDAKMMSTQFIRAAYFEKYGVSIYIG
                     VGIPIPVLDEDMARQVMVRNSQIETNIVDYGSPGHEILGRTDYHSLFSGEISLNGKKI
                     RTAPLSSLKIAREIAERLRSEILSGNFFLTAPVEGFRKTRGLNSLKIRL"
     gene            25442..25852
                     /locus_tag="PK288_02180"
     CDS             25442..25852
                     /locus_tag="PK288_02180"
                     /inference="COORDINATES: protein motif:HMM:NF020940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HPM17753.1"
                     /translation="MKSRRFVLTFPPETTGEPITYNLIRKFDIMVNIVKADVTPGHIG
                     HLVMEMTAPPGILNEGLEYIRGQNVECVPIDRRITYRKDMCIHCGACSAVCFAGALTM
                     DRTTAELTFEPEKCVVCELCLTACPLKLFSIDLG"
     gene            25890..26603
                     /locus_tag="PK288_02185"
     CDS             25890..26603
                     /locus_tag="PK288_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPM17754.1"
                     /translation="MGNRRWMSFRAAWRETDLWVAIDREHYQHEAKRFTLDRIVYYRD
                     ILENYISLRPEFRDSLTPVSVPDGSHPLIISMSNAARQAGTGPLSAVAGATAEYICGD
                     IIEKFRAEEVIIENGGDIFMKLTAPVTVAVYAGDSPLSEKIALRPAPCNTPLAVCCSS
                     GTVGHSLSFGVADACVIACRDGALADAFATAFGNEVKGKEQLGAVTEKALKVPEIISV
                     VIVKDDQVAMGGSLEIKLV"
     gene            complement(26654..27838)
                     /locus_tag="PK288_02190"
     CDS             complement(26654..27838)
                     /locus_tag="PK288_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17755.1"
                     /translation="MKVNRNDLVRFTAVVFLFSMLAHQSCKSGADPTSYPVIPDKEFG
                     SLFYSDSGGISGADGLFSFLLPDRSSVFLLGDCFLGKVVNGTRDMRTPMLRNAFIIVN
                     RELTGTRAIVRGSTDDPHTFMEPVNEPGDSTYRWYWPGHGFIKNDTVYIFALSLVNDT
                     SVVINIRLPEGADDETGRMAEEIFSFRISHIDLLSFSYPDFRHIETQTVNIDYPSSLI
                     DFGNCVMVDKGWIYIYGTRNLPDMSKIHVARVPFSSNIFKSDWEYYTGSSWDKDIRKS
                     MPLDIDISVSEQFSIFRYGRKYILLTQERAGADIYTYISDSPYRGFHNKKMIYHTTEM
                     ESDPNKRVITYNALAHRQYIENGMLLVSYCVNSLNPIDIFEDVEKYRARFIRVPMKLI
                     LR"
     gene            complement(27835..29079)
                     /locus_tag="PK288_02195"
     CDS             complement(27835..29079)
                     /locus_tag="PK288_02195"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HPM17756.1"
                     /translation="MKTTAFIVTSAAVLAGCCTSCGPGASCGQAGPMDPLCFTSEPAP
                     EWTALMERTSGWFAADGIFSIPLDGVEDQQNKNKRTLFIFSDTYIGEVRNNVPLPGNV
                     MVNNTAAWMTGVIPDESVIVFRYNKDNEGNPVSYFIPDNRNSRDGEYFWLGDGFVNHA
                     KKNSLYIFAYHVHKTGPNVFDFEATNVAILKIREPTPKGLGICEQIPTELGFVHPVHG
                     RVYFGSGIFVNTGKAGAPCPDGYVYIYGIAGEKKSLVAARVRPEHFEETGRWTFWNGE
                     AWVAGKENIFPITDAVSNELSVTPADDGRYLLTFTVMGISDKIGIRVGSGPAGPFGEI
                     NEVYTCPEYQEKGLLPYNAKAHYHLSKPGELLVSYNTITLNFWEDIQKDATIYHPRFI
                     TVRYGPHEKTEEKINTDKAMYK"
     gene            complement(29113..32001)
                     /locus_tag="PK288_02200"
     CDS             complement(29113..32001)
                     /locus_tag="PK288_02200"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPM17757.1"
                     /translation="MKKYILLFALISSAAVLSAQKQSMEPVDYVDPLMGTSSPQWTLF
                     PGASTPSGMVKISPDNQLAGWKAGYDYNIESIAGFCHIHSRTMGGLLVMPVTGRLNIF
                     RGSPEDPDSGYRSRFSHRNEKAQPGYYSVILDDYNIKAELTSTTRAAFQKYTYPESDT
                     ARLLIDLKIPTSHGYEITGAGIRKVSSTEIAGFSIQQSLMGAEANRYTIHFVIRLSKP
                     FKSFNGWIGDRILRNAEEINLSYEDKGIGVFLDFSTKDGEEILVQSGLSLVSVDQARL
                     NLETELGPFAWDFTSVREKSRDEWNRLLKTILVEGGDEENLKLFYTSLYRAFAGQTTW
                     SDVNGMYVDMDEKVQVPGNPDYPVYGCDPPGAGAIALFQLRNLCQPDLSNKCVLSLLE
                     TGQKGGWLPEAAEGVEYPATPVVSYGIPMMASTYQQGIRNYDTSMMYKAVVHDLSVAG
                     APHRGGGFAGVVYLEPYLKTGIAGGTADSAAITCGYSFTDWTAAQMAKRSGNIPDFRT
                     FTKRAFNYKNVPPRENKPLSAFVPHDVNGLLISSGRENFIRKLEESIDSSLKSGCSGM
                     TSPDLIKAFLYSGDYRTEQHPLQAAWLFNYTGQPWLTQKLTRDIMTEYRSGKAGAFNP
                     CIDPAQAGALFVMSAIGLFQTDGGTSAEPFYEIGSPLFEKITIRLDNKYYRGNEFVIK
                     TVNNSAENKYIRRASLNRTVLNKPWFRQADLAAGGMLELVMGPVPNLKWGSNPADSPP
                     SMSTRLTPEETKTIMAYDKYAEDLAAWNQALRAYYYHKKEHFESLPNTPNEIIFLGNS
                     ITDNCEWWELFSNPNIKNRGIGGDDTDGILERLDEVTESNPSKIFIMIGTNDLSNGKS
                     VEYIVGNYRRIIERIRQSSPGTRIYIQSVLPTDDGIHYTRRNTEIIRINDHLKDIATE
                     NGLTYIDLFNIFQTENHKLNPQYSIDGLHLNGRGYLLWKDAIIKYVEE"
     gene            complement(32189..33607)
                     /locus_tag="PK288_02205"
     CDS             complement(32189..33607)
                     /locus_tag="PK288_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17758.1"
                     /translation="MYTVLSKLKLFLYPVKQLLIPGFIFGLAVVPYSIPAQEVVREAM
                     EHFLKFNNVPGGWLAADATISLPLPDNNTLWLFGDSFIGERTGEYSINPGKSRFINNS
                     AILETGNEMKTLYAGTPANPSSFIPGEGKDIFWPEHAVIENDTVKIFAVRIKPGNTGV
                     PGFNFIVGTTYKASFRYPGFEYISTTPFKSVTDSSFRFGACVIKSGDHTYIFGVKDTT
                     SGQYTYPLPYLARVSETIEEPWEFYAGPDTWSRNIADAVPVGNRPMSESFYVYERNGR
                     FYLLMHEIWLVGELWILEADKITGPWNTRAAGGIENRFAVITKPKTNITYNLFAHPQF
                     RENGEILISFNVNTTNFNSIYSDTRNYRGRFFWLDPEAAVAVTKPDTIKLFNTPVAVD
                     PVPDDNTHARIIYHPATGIIHIRNVNTPSMLSIYGLDGRLYLSRMIKADTEIDCSRFP
                     VTLVIARLKWDRGVETRKILLR"
     gene            complement(33619..35958)
                     /locus_tag="PK288_02210"
     CDS             complement(33619..35958)
                     /locus_tag="PK288_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17759.1"
                     /translation="MKKPILIMVLPVVMILATCKSHTNDQSSPYNTIPSLDELSAFWV
                     STDTVEMEPSIRNFRGQALLNRDMTSISWFVSAPFSGGYHSGTLRINGKTPKVNSWRW
                     QPYQGLRKGSHDNLEILSSTRMLFDKDAIMWEVEITNKDTIAREISPELDLIGFVSKF
                     GGDWQWWYPYPSMSGTVTNRNEEVENIREHLGEQVFEKEGLSWELVNGKPMLQKVLMK
                     WPSDEEIMNSAKFTTSVENNVIYIRDTESEAITSFSVVTAPDQISPKKCGATVSWKFR
                     MEPGATRKIRYLMSYGDNHEIIKQNVLEWSAGFEGYFRGIKDSWEEKWKSIFQPGNSF
                     ISGCFPVLETGDLSARKVYYTSPLTMLYMTNTNLPQHSKVFLTGGPRWGASITFFWDI
                     TLWSTLWAVVDPVMMKEHISSWILIDPSKYYGMDNFGGKGVGNGYSANYWALFQMIRD
                     YIAVTGDYAFLDETIGNGTVLQHLDDYAMNWERISLYGTEGCTDDIYKLADFGDDEWN
                     LLECVPTYKHIVPSFNIGYAWMMREAAKLHLHRGERQKADSLNTLATEMIGRILKLYA
                     GNGVWNSLYPGNKTIQVRHCLDFAFFGKYIPDDIPAAIKSEMLEFLYNELITDHWMRA
                     QSLSDPAADKSDRADHGPFGAFDGWPVDIMDALTQMGYPEKALNFYHAVEPVTNEGIW
                     AQAHELWGENKTNKKALVRIAERDWCNRESSSGVAFAQTMLKDFFGFYPDLNGTVIKP
                     PKDFTFDGRMHHVYYRNEYYTIEYKSGKPVMIRERNRKQ"
     gene            complement(36150..37616)
                     /locus_tag="PK288_02215"
     CDS             complement(36150..37616)
                     /locus_tag="PK288_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM17760.1"
                     /translation="MMKKIIIILTVLLSLGSCKGFLEQDLRSELNYDNYFLAEKDLVS
                     FADGMFGGLITWTWTGGGLFFNNYWVLQDLASDNCYETGPSIDMLDLSQFTFDENNAT
                     VYYIWVGCYEVINSTNVLLEESENIQHYSRPEIKDHLRGEAYFLRGMLYFDLVRLFGD
                     IPLQLFSTKTAAETQIPRTPVAEVYESIISDLEKAEQLLRINPFPNRVQGMPTSLAAS
                     ALLAKVYLTRGALTNNAGDFNLAKTSLEKVIGNYTLEPEYADIFKLKNANAGEIIWAV
                     NFSGTLGEGWTTNQFIVRLMPTTVAANGTRNGQGWERPLDILYNSFAPGDKRKAATFI
                     TEFEGEIFNGPYIRKYWDQEAEGGRQNGESDADFIYLRYSDVLLMYAEVLNEINNGPT
                     AEAYNSINAIRNRAGLDDLAAGLDYQAFKDALLQERQWEFVMEGHRWFDLVRMGKLFE
                     RIRIAKPTANIQPHHILFPIPQKERYLNPNLTQNNGYN"
     gene            complement(37901..>40291)
                     /locus_tag="PK288_02220"
     CDS             complement(37901..>40291)
                     /locus_tag="PK288_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM17761.1"
                     /translation="ARGANGVILINTKRGKKGDFQVNLNLSYGYQQLAKKIRLLNARE
                     LAELGNDAVTEARKYYPEIAYGSNFAVPSRFGKGTDWQDQIFRTAPLTNSQISLQGGA
                     GNIKYFLSANLVTNKGIIHNSDFNKGNVRFNLDTDLSEKVRTGINLNMSQSVSHGVTT
                     GVPNVASSVTAMALLFNPGQEVYDSTRVGGYTYESNTINNIPNPVAEINETDIVITTD
                     RLIGDYYVDWDIIKNLQFKFKVGADAFFNKEQQYIPSFIKRGQDKGKGSNVDIKGYTW
                     LMENTLTYKFAADRHHFNVLLGQTAQKFVSERAEIAVERFEDERLTYYNLGMGLDKTI
                     MSGYSSWAMLSGLGRVMYDFDSKYYLTVSGRLDGSSKFGTENKWGFFPSVSAAWRISK
                     ENFFSGVKTINDLKLRLSAGSVGNEGIPSGATLSLMGTHPYFYGEGSNAEVIGTYVYS
                     LRNNDLKWEVTRQYDAGIDLSLLKSRLEFTAEAYLKNTSDLLLFLPVNRSSGFEYVWA
                     NVGDLQNKGVEFSMNTVNLEGDFRWNTRFNISFNRNKVTNLDKSDRIYGVSVLNILDW
                     TMITEGEPIGVIYGYKSNGIIQRNEELSQIPFFPSKIARYGDRKYIDRNNDGKLTVDD
                     YFKLGNANPDFSFGLRNTFSYKNFSLDIYLQGDYGNEIVNFNRFQLESFDGFQNNSAV
                     ALQRWTETNPTNKYPRANASPHGTIMSDVYVEDGSYVRLKQVSLSYTLPQKLLNSIRT
                     RNIQLSLSGYNLYTLTRYSGYDPEVSIFGGSVFGKGADYGAYPTARSLLFSINATF"
BASE COUNT        10441 a         9326 c         9539 g        10985 t
ORIGIN      
        1 gtttgaatgt gggaaggtgt tctccgaaat ggatgtaacc ctcatggtca agatagatgt
       61 gctgacggcc ggtgagagtg cgccatggta tcagatgttc aatgttcagg gtgtatccag
      121 aataggccct gccgtttgtc atcagtcccg accagatggg tgaattgagt agtcttcgcg
      181 gctgtgcctg caaatcatca aagcggagag tgatatcgtt gtttttcggg tttatcgggg
      241 taagatcaat cccggttttc tgctcaatat cggagaaggc ccttccgctc agagtaccgt
      301 ttgtgaccga tgaaagcaga agtatggcat taatggcctc agtatcttcg cctattgagg
      361 gataaatctg atcgccgacc cggtttacat ggtttttatc attcagcatg cggtcataga
      421 agtcatcaca gcggaatgat atgttatgta cccccagcct gctgcgtgca ttcctgccca
      481 gactgataaa tttctcatat atcctggtgt agtctctttc gacggtgatt atgttgtgca
      541 ttgacttccc gggaacaggt tcgcactcgc cggtaaacca gtcttttatg actggctgcg
      601 agatctctcc cgccgtatcg tgcccgatcg gcctgtttac gatatcaaga accggttcgg
      661 ggaaatgttt tctggcagtc tcactggtca ccctggcaat ctcacggaat attgcccagt
      721 cactttttgc ttcccacacc gggggaactg ccggcgccag cgggttgatg aatgaatgca
      781 tgtcggtggt gttcaggtca gccttttcat accatgaagc tgccgggaga acgatatcgg
      841 aatagagtgc cgacgtgtcc atcctgaagt tcaggtccac caccaggtcc atcttgcctt
      901 caggcgcatc ttccctccat tcaatctctt tcacaaactc ttttgccact tggtcggcta
      961 tctcatagtg atgcgtcccg aggtagtgct tgagaaaata ttcatgtcct tttgcgctgg
     1021 tcatcaatgc atttccacgc catatatacc ataccctcgg gaaattcacc tcgttgtcag
     1081 gatcggcaac cgagtagttc agctctccct ttcgcagctg gtcaatcaca taatcctgaa
     1141 ttgccttatc atcaagagca cccgcctctc tcgccccggc agcgatttcg aaattgttcc
     1201 tgtcatactg ggggtaaaat ggcatccatc cgtttctcac tgccttaact atcatgtcgg
     1261 ctgaatgccg ggaggagaac tcattcttcg gaaccgaatt atagtcagcc tggttgccat
     1321 catatcgcca ctggtcagag tggatataat gccatatcgg ggattgctgg aacctggacg
     1381 gaatattcca gtcgcttccc gacatgattg atccccatga atcaaagggt gcaagtttct
     1441 cctgaccgac atagtggttc atacccccgc catttacccc gacgcatcct gtgaacatca
     1501 gggccattgt agctgcccgg tatatcaggt tgctgtggaa ccagtggttt atcccggcac
     1561 ctattattat catgcacttg ccctgcgtgt tctctgccgt cagacaccat tctctggcaa
     1621 actgaatgac ggtagctgag ctaatgccgg taaagatctc ctgccatgcg ggggtatatg
     1681 ccgatccggc atcatcatat gaagagggat agtcgccgcc caggcctctg tcaaccccat
     1741 actgccccat aatcaggtca tagacagtgg taaaggctat tttccctctc ctggtttgca
     1801 tatacctgac aggtacaccg cgttttgatt ttgtatcgcg ggcgaaatct gtaaactcca
     1861 cctgcaggat tttatcactc ttttcaatga gtgtcagcaa tggggtatac tctgttccgg
     1921 tctccccgtc cctgtatttc aggttccatg cccccttctt atcctgccac ctgtggcctg
     1981 agctgcctga ggggcagatg aactctccgg taccctcatc aatgttgagg aatttccagt
     2041 cgccgttctc gatatcggcg tatttctcaa gctgattggc tctcagcaat ctgcccggca
     2101 ccatccggcc gccctcctct attatctcca ccaggaaggg agcatcggta tatttcctgg
     2161 tatagtcaat aaaatagggt acctgtttgt cagaatgagt ctctttcaga ataacgtgtg
     2221 ttaccgccat ccagaaagct ccgtcacgcc cggcatgaac cggtatccac tggtcggcat
     2281 attttgagac catactgaaa tcaggggaga aaaccactgt tttggttccg ttgtggcgtg
     2341 cttcagagaa gaagtggaca tcgggtgtac gggtcatgcc caggtttgca cccattactg
     2401 caatgaattt ggcattatac cagtcggcgc tctccccgac atcagtctgc tcaccccata
     2461 tctcgggaaa tgagctgggc aggtcacagt accagtcgta gaagctgaga tgcaccccgc
     2521 caaacagctg cagaaacctt gacccggccg caaagcttat cattgacatg gctggtatcg
     2581 gcgaaaaccc tactacccgg tctgctccgt attttttggc cgtatagata tttgctgcgc
     2641 ctatcacctc cagcacctct tcccaggtgg ctctcctgaa tccgcctttc ccgcgtgcct
     2701 tctggtattc tcttcttttt acggggtcat tctggatatt ctgccaggcc aggagagcat
     2761 cgcccgtcat tttcttctcc tcattgaaca gatcgatcag ggctcctctt atcatcggat
     2821 atttaaccct gatcgggctg tagagatacc atgagtagga tattcctctc tggcagcccc
     2881 tgggctcata gggggggagt gagttttcaa gcatcgggta atcgagggcc tggaattccc
     2941 agacgacgat tccctccttg acatgaatat gccagccgca tcctcccgtg cagtttaccc
     3001 cgtgagtgct gcgcaccttc ttgtcatatt gccagcggtt acggtaaaac tcttcccatt
     3061 gcctggtctc gggggatatc atatctttta tccaactcat gattcctgtt ttttatcagc
     3121 acactttttt gaatctctga aatagtctca ctgaagattt tacgggatac gtctcctgtc
     3181 ataaagaata tagatgtgcc ccaggagtat cagtgcacat accagtccga gaccgaaaaa
     3241 gatcagtcca cgtttccctg ccggataggg ctgctccccg gctgccttga agagtgatat
     3301 caaggcctga ctctcatcct cgttgatttc atggttgagc attgcccttt tcatgacagg
     3361 gaagggaggg ttcttgataa tggcagcaat acccggtgct cccagtttgg catatgagcc
     3421 ggtaaggtca agtgccattt ttcctcctcc caggacagac tggtcattaa tgttgtggca
     3481 tgagatgcag ggtggtgcat tgtttgcaaa cctggtatgg ccataaaaga gcgctctccc
     3541 ggcaggcata tgttctatgc tgtagattat tgccaggctg tcttctgaga tggcagcggc
     3601 gccaccaaca gcctgaaggt cctggtctga ttccctgata aaatcgatta tgctgaggat
     3661 ctgtgcgtcg gagagccggt tatcgggcat ctgtaccctg ttgtactcct cgaaaatagc
     3721 gactgccagg ctgtcgcctg acttgatgaa ctgttgcgag gagcggataa agcttatcag
     3781 ccactcattg gttctgattt ggtagatgcc gcttaaatcg ggaccaacca gccttccgct
     3841 gcctatggta tggcaagcgg tgcaggttga ttcaaatatt tccttgccgt cggattgagc
     3901 ttgtaagaga tgatttgccg ggaaaagaaa gagacaaatg gcaaacaggg aaatcttctt
     3961 catttctatt ttgataaaat agtgaaccaa gattctgtga ctgaaacagg tcagtcgttt
     4021 tgttgccctc aaatactccc tgctaccatg ctccgccagc ctctcagtta ctctcccggt
     4081 taaccggttc ccacttcatt ttgatactat tcaagggctt cctgacaaag atattttttt
     4141 tttgaaattt tcaaacgaga gtatcataat tatcgtcttt ttttatctgt tatcgggaat
     4201 gacctgtttc tgaggatatt ctgtctcttc cggcttccgg atggtgtttg ctgccggggg
     4261 ttctccagtt tccatcagat atctttcttg cctttatgac tacgaaactg ccggtgccat
     4321 atcccttctc agccttctgg actaccctta tctcttcagg cagaccgaat atggtctgaa
     4381 aggtttccat gacctcgaag ttgctgtcag ccaggtatct gtagagctca ccggtgctat
     4441 agaagacagc atccttatag aatacattct gctctttaat ggtgaggtac agtttgccga
     4501 cagggctctc cctgtcaaca aagccgagaa ttatgcagcc atcatctttt aggatacggt
     4561 aagattcagc cagtgccttg ttcaggtcat ccacaaaaca gattgtggtt accatcagga
     4621 caaagtcata gctgctgtcc ggcaggggaa ggttctctgc tacgccatct gttacagaga
     4681 gccctctctc ccgtgctttt tctctcatgg cttttgacgg ttcaactccc tccttaatcc
     4741 cgagcggcag ggcaaacttg ccgcttccta ttcctatctc gactcctctg cctttgtcag
     4801 gcagggcctt cttaacggct gcaagttccg acaggtacgc atagtggttt tgcgaaaacc
     4861 actcctcata ctcctccgga tattgatcaa agggttctgt ttttgccatg tcagttactc
     4921 tgtttcagtg aattgttgaa tcataaccat taattgactc atgaatttac gaatctttga
     4981 ggtttgaaac aacccgggga ggtttcatcg ctgctgtttg cgatcctgac ttgaacttcc
     5041 ttgttgactt tggggaaata tgttaaattt agtgaagcgg acgcagttca ttttattaac
     5101 gctgataatt gagaattaaa acctgtatat acctgctggc aggagtgctt ctttcagggt
     5161 tgattagcat gaactcatgc aaccagtcaa ggcctgagaa agtccgtgtg atcggacaga
     5221 aaatagacag tctgaatggg gtctatgtct tttataacgg agcggttggc aacgtttcag
     5281 ggcgcaatac aacaagagat gggtataatt taggactgaa ataccaatgt gtggaatttg
     5341 taaagcggta ttactatgaa tacctggacc ataagatgcc tgacagctat ggccatgcaa
     5401 aagattttta tgataaaaca ctgggtgacg ggcagaaaaa caaaaggcgg aatctgatcc
     5461 aatactcaaa tccgggcagg acaaagcctg cagttagaga ccttgttgtt tttgacggca
     5521 ccgcattcaa taaatatggg cacgtggcaa ttgtgtcaaa tgtgacaaaa aggaaggtag
     5581 aaataattca acaaaacccg ggatcaaacg gaaactccag ggaaagtttc ccgctccgtg
     5641 agaaaggtgg aaaatgggaa attggcgata aactaatatt gggatggctc aggaaggagt
     5701 gagcccgacc cgaaaaagat ctgcaggttg aattttgtcc ggataacaat ctttttaatt
     5761 tatgacctat gagcagatac aattacataa tagacaaaaa gatggtatct caaaatgcac
     5821 cctcctgagt ttcccttcaa atctcttatg tcacatagtt aaaatttctt ctgtcagatg
     5881 aagtctttac tattgcactt aaccagtggt ctgaaaattg gggagaattt tatacatatt
     5941 aggctaaaat gttagcttta gggaagatta atgggaaaat atatttcaat gtttcaattt
     6001 aaacaatatg cgtatgaaac caatgacttt gtttgtcctt gcatttgcac tgatcttttc
     6061 aggcataagt gcacagcagg tggtatggca gcccgatgtt atcaaaagac tgaccccgga
     6121 atggaaaggt gaacggtatc ccgatggaag gccgaaagta cctgatgcac tcctggaacg
     6181 gcttaagaac tgttccttcg aagagataca ggcttatctt ggcatgcatg gttatcggaa
     6241 tgtattcgag aattttgcca gcctgaatga aaacgggtgg catataatac accccgacaa
     6301 ggtaatgaca ggtcgtgccc ttacagcaca attcatgccg ctcagacccg atttcaacaa
     6361 ttatgttcag gcgcaggcaa aggaagaagg gacgcatacg ccggtgacca attatgcccc
     6421 tatcattaaa ttgcaggaag gggatattta tgttgccgat tcatatggta aaatggaagg
     6481 aggtaccctt attggctcca acctgggcaa tgcgatagcc aatgcatcaa aaagaggtgt
     6541 gatatttaac gggtcgctga gggattatga gggtcttgaa gcaattggtg agaatttcaa
     6601 cgggtggatt aggggctatg atccctcggg tattcagcag atgatgacgg cctgggttaa
     6661 tgcacccatc cggatcggga gagtaactat tttacccgga gatgccatcc tggcaaagaa
     6721 actcggcatc gtcgcaatcc cgccccatct tttgctgggc tccgtacttc acagtgaata
     6781 tattacgctg cgtgacgaat tcagtttctt atgtattaaa acaaaacgtt ttccctatat
     6841 aaatgaggct tttgtcgtgg accatgatgt ttatgaaaag gcctttaacg aatggctgga
     6901 tacgcagaac ctgcctatga caaggaatga actgaactcc tatcttaagg agatggagga
     6961 attaagggcc tcttcccgca aaaaataatc aggcagcaat ctgtaccggc atttcaaaac
     7021 ctgacactct tctgccaaga accatgaata actgatcagt cgggcaggtt gtaaggcaga
     7081 agttcccggc aacggatcgg gatgatccgg ccgggtgatg tgaaagacca gcattgttat
     7141 caggtttcta aacattttat caggcaggct gttaattgta atgagcataa cagttgactt
     7201 ttaagaatct ttcttctatt tttaaattga gatattttta tagttagggc aattcaggct
     7261 tcatcaggtt gaaggaatat ttgatgtgtt gatttaagat caatttgcgg gatcatatga
     7321 aaaagttatt atcagcagca ttgattttgg tgctatggtt gcttgcaggt cctggaataa
     7381 aggctcaggg aacgtcattg gaggaagaac tgatttacct tctgggttca aaggacagaa
     7441 ggcatgcggc aatgctggca agagatgtca gatatgtaga ctataccgat ttgcatgaca
     7501 ggagtctact catgtatgcg tctgcaaacg ggtatggcgg catatgcagg atattgctca
     7561 aaaagggagc agacccgggc aatcaggccc tggatggtgt cactgccgca atgtatgctg
     7621 cctataacgg acataaaaga ataatcaggc tcttgcttaa agagggaatt aatcctgata
     7681 cacaggcaat tgacggattg accgccctca tgatggctac ccagaacggt tacagaccga
     7741 ttgtaaaatt acttctcgac gcaggtgcca atcccaatct tctgacaaat gacggttatt
     7801 cttcccttat gctggcagcc cagtatggct atgatgaaat tgcccggatg ttgatcgata
     7861 agggcgctga tgtaaatttc aggaatatca acaatgtaac tgcactgaac caggccagcc
     7921 tctcgggatc ggctgaaatt gcaaaaatgc ttttaaaaag gggggcagat cccgattcca
     7981 ggaacaacga agggatatca gcactcatga ctgcctcgct tatcgggcat tacgaatttg
     8041 tgaagacact tgttgaatcg ggggctgatg taaacctgaa gaacaaggac ggagtcagtg
     8101 cccttttcct ggcttccctt aacgggcaca ccgaaatagt tgatttcctg ctcgccaggg
     8161 gagcaaaacc tgacacgcgg gataccgatg gcacagttgc actgatgatc aatgcacggg
     8221 aagacattgc cgaagcattg atacagaaaa tctatgaagg aacaaaggat gtttccaaaa
     8281 gcggggatca tatgatcgtt ttgttgtcaa aaaccgaaaa cggagatctg gaggctgtaa
     8341 aattattcat ggacaggaga gcaaatgcag ccatgctggc cagttccaat aaagctgcgg
     8401 catttatcca ctcttccggc aatggccata tagcaatcgt aaatcttttc atcaagaaag
     8461 atgcccgcgt tgaaacaaat aaaactgccc ggtttaccga tctgatggct gcttccctca
     8521 acggacactc ggagattgta aaacttctta ccgaaaaagg agcaaagccc aaccttaccg
     8581 gcaatgatgg cgccactgca ctgatcctgg catcgcaaaa tggcaatgct gaaatagtaa
     8641 agctgcttct ggaaaaagga tcagatataa accttcagcg gtccgacggg ttctcagcac
     8701 ttatggctgc agtcaaggac ggacatgcgg atgtcgcaaa actgttgctg gaaaaggggg
     8761 ctgatcccag gctaaaggac attatgggta aaactgtcgt ggattatgca gaagatgctg
     8821 aaatcagaaa actgtttgta aagtaggaaa aagatatttg ccgatacccc cggaaagtgc
     8881 aaatcccatt aatacataat gcacctttat catgaaaact atcagagctt acacattttt
     8941 gattttgctg accggcatcc tggttattta ttcctgcgac aggaagcctt atatattaaa
     9001 ggatggttac atatatgttc agaaggagga caggcggaat gagtctgctg aatggcagct
     9061 tgtgtgggaa gatgaattcg acagggggga actggatacg acaatatgga gccgtatcgg
     9121 ccttttcgaa actcctaaat ggaaagtccc tgtggaaaaa tggagggagg ttacaaactg
     9181 tttcagatat attactgcca ccgatccgcg ggtggttcag tttgatgagg gaaacatcca
     9241 tctcagaggc attgtaaatc ccgattccct gtcaggcgac ccccgtcctt acctgaccgg
     9301 cggtatctac tcctggagaa agtttgcttt ccagtacggt cggatagaga tcagggccag
     9361 acttgaccag gcatacggca tgtggcccgc gatatggatg ctgtcggaaa aggaaattta
     9421 tcccgaccag cataatggtg aaatggatat tatggaaaaa ctgaatcatg atgactttgc
     9481 ctaccagacc acacacaatc attacaccat tgcactgaag cagaacgagc cgaagaagca
     9541 tgccaccggg aagattgata caacagatta caatgtttac agtgtaagct ggtatcccga
     9601 caagctcgtt tatgccatca acggcgtcgt atcttacgaa tatccgaagg tcccgggcgc
     9661 gggtacttat caatggccct ttgatcagcc tttctatctc ctgatcgacc agcagatgga
     9721 acatgagtgg cccgggaaaa taacaaatcc aggagaactg ccgatcagca tgaccgtgga
     9781 ttgggtcaga ctgtatcagt aaaccattct tgtctcgtgc agggaaatag cctgaattct
     9841 aatcagcagg catatcctcc gttcttatgc ttctggcacg gtcaagatat tcattgaaag
     9901 gctcaatgtt gaattccctt atcatttgtt ccgaacttac gcttttattg ctgctcgtta
     9961 caataacccg gccatttctt gaggtttcac tgatctggac agtatttccg tatttcgatt
    10021 taagggcgac aagcttttcc agattattgg caagcgacac cgttttataa tcgaggttga
    10081 tattggcaat tgcataatca gcagccttgc tggtagtggc tattaccttt cccaggggat
    10141 ttctcacttc ggaggggaaa tcgcgataga ttgatccgat gaaatatgaa ttgcatgaaa
    10201 gagcccataa attctgcagg acacctccat gcgacatcga cgggtatatt attatatcag
    10261 tttctgtgct cgaatatttg gcgagtagct catcaaagtt aagatcaaaa cagatggcga
    10321 caccgacttt tccaaaatca caatcaatga cagcaatgtc atcacttgcc cttatcccgg
    10381 tttgaatttc gcttattgtg ggatagttct tgtcgtaaat gcctgcaata gctcctttcc
    10441 tgttcaatac catgcatgaa ttgcgccaga ggcccatcga atcctggcga cccatgccaa
    10501 aagcaatgta gcatttattt tccctggcaa ctgaggagaa aaaatcgagg acctgattcc
    10561 cccttacttt catgtactcc acacctgcaa ttgacagatc ggcgaattca ggcagaacga
    10621 taagatccgg ccgggctttc aatacctttt tcagctcctt ctcccagaag gaaatcacat
    10681 agtccaccgt tgcctgcaga ttgtcattgt ccgtaagagc gggagtgttc ccgattgtcg
    10741 ctaccgtaac cggccgttca gagtaaacaa ggtcaggatt aaatatatcc gccagcagca
    10801 ggaaaaggcc agccatcagt gctgtgaaac aattgctttt catcttgcct catgttttgg
    10861 aaggccggat caatatcacc aatagtgatt gaaacatcag tccggcatcg ttttatccaa
    10921 agtctccatg cagattcaca aatatcccaa gaaatgccgg aaagtgttcc ctttgcagtt
    10981 ttaaaagacg ttaatcaatg ttaaataacc gtaattgaat aattcacaac agacagatgc
    11041 tttcatcatt attcaatcac gacccggtcc ggaaatccgt aagatcagat ctgtattgca
    11101 tcctcttccg ggtaatgctg aattccgttt cagtttgacg aaaacaaaga tgcgggatcc
    11161 ataattttct tttatctttt caccagaatt atagccggat gattctgaac caatcctttc
    11221 aggtcagttg tctggccggg agagagcggg aaaaatcagg gcagaccggc agttgcctga
    11281 tctcatcagg atttatccgg cacatgtcag ctgcgattgg cctgtacggc aggccctgcc
    11341 ccggtgaagc gcggatcatg gagccttttc attccgtgga aacggcaccg gatattgtac
    11401 aaccgggatg tgtgaaagaa ataacagttt aacttataat aaggttattt acagaaaatt
    11461 taaacagatc tatgaaaccc tcatgataca aaatcacaaa caggtatgaa aataaaattc
    11521 atgtggcttc catatgaacc atctaatatt cagtagttta taaatttatt ttcagatgaa
    11581 aaagcaacta attctaactt tgattctagt gatgtcggga agcctgaatg catttgtttt
    11641 cccgcaggca tcacctgatg aggtttttac cgttaaggga ttcagctgta cctcaccccg
    11701 acgccatgag gtggaccgtt tcgtgcaatt cattgacagg gaacttgccc cgcgcggcgt
    11761 gaacactcta gttctctttg tttgtgacaa ggatttcaat taccagtttg aaagccatcc
    11821 cgagcttagt gatccttcca tgctttcaaa ggaagacgtg aagaaaatcg ttgcatcctg
    11881 caggaaaaac aagatcaggg tgattccgca gatcaacctg cttggccacc agtcgtggag
    11941 ggaaaaaccc aacatactgc tggaaaagta tccccagttc gatgagacac ctcatatcag
    12001 gctgcctgag aaatatgtgt ggcccaatga tgacgacctt tattgcaaga gctattgccc
    12061 ccttcatccc gatctgcata aggtcatctt cgcacttgta gatgagatat gcgacgtatt
    12121 tgagacagat gcgtttcacg cagggatgga tgaagttttc taccttggcg atgacaaatg
    12181 tccccgttgt tcaggcgttg acaaggcaga gctttttgcc ggggaggtga acaggatccg
    12241 ggatcacctg gctaagaaga acagggaact ctggatatgg ggcgatcgtc ttattgacgg
    12301 aaaaaccacg ggtatcggct tgtgggaagg gagttataat tatacctgga gggccgtcga
    12361 catgatccct aaggatgtag tgatatgcga ctggcattat gaaagggctg atcatactcc
    12421 tgtatatttt gccatgaaag gctttcgcgt tatcacctgc accgggaaca agcctgaaac
    12481 aagcgttgcc caggcagaag atattgtaag gttcaggacg aacacaacca gtgagatgaa
    12541 accaaggtat tacggaataa tgcagaccgt ctggtcatcc accgaccagt ttatagatgc
    12601 tttttacggt aagcagccgg ccggtcctgc cggaagaagg aatccggctg atggtttcaa
    12661 ggctctgtac accaggatca atgagctgga gcagaagaag ccatgaatcc gatcctgata
    12721 gctgactccc tgattaagag atacggcggc ataacagctg ttgacggact ttctttcgag
    12781 atattccgcg gggagatttt tgactttcac cgacccgatt gacagaaaag tcctggcact
    12841 ggcactaaac aggactctca tttagcgttc cggcttttca ggcggaaatg taatgatctg
    12901 gctgaagtgc ccacagtttc cggatccatc cggcaatgat ttttagcgct tcttttttcg
    12961 gcgttccaca tgtgatattt tttaacttga aatgctgttt tttctccagg gcatgtaaga
    13021 ttattgaaca aaaagggctg aaaaatgctt atatgaaaaa cctttgtttt gaaaaggttt
    13081 ttcacatgat ggtttgataa gttttaaaga caatgggaac gcaagataaa cttgatatac
    13141 taaagcataa actgggtgaa tacaagagtg cagtgatagc cttttcaggc ggggtggaca
    13201 gcacattcct ggcacgggtg gcaaaagatg tttacggcga caggctgatg ctgataaccg
    13261 cctcatccag tacatatcct ttttatgagc tggaggaagc cagatcgctt gcatcgatga
    13321 tgaatataaa gcataaggtg atcgtttccg aagagattga tatccccgga tattccgata
    13381 acccgcctga caggtgttat tactgcaaaa gcgaattgtt cgggaagatc tcattcattg
    13441 cccggcagga aggatatgag gtgatattcg acggcagcaa tgccgaagat ctgaaagact
    13501 tcaggccagg gctcagggcg aaaaatgaga aaggtgtaat ttcaccactg gctgaatgcg
    13561 gattcaccaa ggcagaaatc aggtattatt ccgaggccct gaatctgcct acggcaaaca
    13621 agcaatccta tgcctgcctt gcatcacgat tcccttacgg ggagaaaatc accattgaaa
    13681 aactcgacag gctgtcggtg gccgaactgg aattcaggaa tctgggcttc agccagttcc
    13741 gtatcaggag ccacgataat atggccaggc ttgaattcag accagaagaa atggacaggg
    13801 cctgggaatg ccgcgacgtt ctgacgaaga tatgcagaaa cgcaggtttt gtatatgtgg
    13861 ctatagatct gaccggatac cggaccggat caatgaacga agcgttgacc tgaaaacctg
    13921 aatgccggag tcaggttaat caatgcgatg atcctgttca atggcacttt accggggcta
    13981 ttatattcat tttttcatct tgataccacg tggaaacagc cctgctgcct ttcatatgtg
    14041 gcccagcatt tgttttcctg cttcaattca tagattattt ctgcaagagc ttccttcatg
    14101 taccatttat cacaatccgt aaccttatat tttctgaaca ggaaacgcgc ataggaaatg
    14161 tcaaaggcat ttccgtacag atgcgggctg tcagcagagg catttatgtt cgcttttccc
    14221 agggcagcga tattctcaga cgtgcgtgtc attgatgtta tgacgaacct tgatccggga
    14281 aatccttctt tttccgtttt cttcctgaac cttttgccaa tctcatcaag cagcctttta
    14341 ctgtctgatg taagataggg aaagctgtgt gtcatgtttt caatcctgta atacctgccg
    14401 gaactgacct ttctgagctg tcgggctgat accctgttcc gtaaatcaac ggcatcagag
    14461 catttctcta ttccctttgc cctggccttg tctgaataat caacgatacg gtcgttcagc
    14521 tttttactgt aaacctgctg ataattcctg catttactgc tgaaaaaatt cagtatcaat
    14581 gccctgtttg aatatggtaa aaagattgcg cacaatagca agatcgttat gaaaaaagac
    14641 catactttcc gtctgtattc ttttcgcaga cgtactcttc cgattttcat aaaaatgaat
    14701 ttacaggcaa aaataaagat atggccgcgg actgaccccg ggagatattc acagaatccg
    14761 gattgtatta acatcccggt agaacaggct cagggttctg gtcccgtaag aagctgaaat
    14821 ttgtcagatg gcagctgtca ggatcatgag aatggtttgc tgtaacatgt gatgaatcat
    14881 acaataaaga acagcaatat tgatttttcc gcaggaataa aaaggttact tttgttgatt
    14941 cgctgataaa atgccggcgt aatgtttttt ccccggcaga tacgggtttg ggatttccgc
    15001 cgggaccata tacagattac ttgcaggcta atacattatg gaaacaagtc atggtagaat
    15061 taattgatga attcggggaa cttcttgaaa acaaggagtg gaaaacttta cgaaaaagac
    15121 tgtccgaata tgaagatctg gatattgccg gacttattga agaattgcct gacgaggatg
    15181 atattgttgt tttccggtta ttatccaaga agcaggccaa gaacagtttt cagctccttt
    15241 cgcatgataa acaggaacag ataatcgagg gactggcaag gaacaccacc aaactgtcgg
    15301 ccctgctcaa cgaccttgat cctgatgacc ggactgcatt tttcgaggag ctgcccggcg
    15361 aagtttcgca gaaacttatt cagctgctgt cgcccgagga acgcaagata gctgtcagtc
    15421 tgctgggtta tcccgaagaa agcattggga ggctcatgac acccgagttt gttgctgtca
    15481 agtcacattt tacagtgggc cagactcttg atcacatcag gatcttcggc catgactccg
    15541 aaaccctcaa cgtggtttat gtggttgatg acaaatggaa actgatagat gacataaaga
    15601 tcagggaaat aatacttgca gatcctgacc agaaaataat ggatctgctt gacagccgct
    15661 atgtttctct caatgcatat gatgaccagg aatcagccat tcgtgttttt aaggatcatg
    15721 accgggttgc cttgcctgtt actgattctg agggaatcct tcttggcatc gtaactttcg
    15781 atgatatcat ggacatagag gaggaggagg caaccgagga ctttcacaag ttcggttctt
    15841 tccaggatgt gatcattgac cccctgaagg ccaggattcc tttcctgtat cagaagcgga
    15901 tagtctggct gactatactc gtattcatga acattttctc aggagctgca attgccggat
    15961 ttgagaatgt aatacaatca acggtgtcac ttatgttctt cctgccttta ctgattgcca
    16021 gcgggggtaa tgctggctca cagtcggcga ccctgatgat ccgttcgctt gcagtgggag
    16081 acgtcgaaat gaagcattgg ttcagacttg tgggcaagga gttcatcgta tcatttctgc
    16141 tcggcataac catggctgtc ggagtttccc tgattgcaag cgtcagggcc cccgagatca
    16201 tccttgttgt tgcccttacc atgatccttg ccgtgatggc cgggagcctg ataggcctta
    16261 ctattccgtt cgtctttaca aaactgaaac tagaccctgc cacggcaagc gcacccctta
    16321 taacttcaat agccgatata gttggtgtgt ggatttattt ctccattgct gcctgggtat
    16381 ttggcctgtg aaacttttcc ggatctatgg ttccgggata atttaagaac atccgtgagg
    16441 caggatcata tttcatcatt tcttcatgat gtatgtttaa ttttgtggta tttatgatct
    16501 gcggacagat attcaggcag ggctaaaaca tattctgaca caattcgtag tttaaagagc
    16561 agaattacca tgaaaacggc aaaaaagatc cttatgcttc taatcacgat atcggtgacc
    16621 tcatttctac tgaacggaca ggcagtcagg gttcatcagg acacagctgt aatggtccgg
    16681 caagagggtc agactcagga acaaacaggc gcagctgagc gtccgggccg gcaaaatcaa
    16741 acctggtctc agaaccatgc gggacaggat ggccgggccg gcaaccggac aaaggtatcg
    16801 gccggaaata gcggtggatc acagccacgg gaagccaatg ccggagaagt aaagaagatc
    16861 cggggagcca gacctgactg gagcagggcc agaggagccc gtccggcatc ggttgaaaga
    16921 ccatctggtt caagaatccc caaaggcgca ggcaaaccgg gaggtgcaaa aggacccggc
    16981 aggcgatagg tataacagga atgaatgtca tgattagaaa caggattcgg ttatatacct
    17041 ttgccggcag tctcgtactg cagacgcttg ccgtatatgc ccagacacaa cctccgtctg
    17101 ccccgccagc ttataaaaat gcaaccgata ctgcagcaca gaaccgtatg cattccttct
    17161 atgcaggtac aggcggcggc aacaacatgt tgtatctcgg atccaccatg tcacagggtc
    17221 atggtttcgg atttgcctcg gtttcatacg gattgatgga caagatttat ttctctgcta
    17281 caggttatac agtcaacaaa ctctctccat tcacagcctt ttacagcctg ggcctcagtt
    17341 ttaaccatac cttcaattca tggttcgata taagcgcagg cttttcacgg tacaatgtct
    17401 cagcaccact gagagatact ctttttgata atttttccta tgccgatgta accctgggat
    17461 tcgactggaa aatactgtat tccaaaattt cggccggaag tcttttttcc ggcgataaac
    17521 agttttatct gcaaacaaga cattcaagat attttgaaac tccatctttt gcgggaggga
    17581 aagcattctt ctcgtttgac ccttacatca atattttatc ggggaccgtt gttagcattg
    17641 aaacttcagg cgatgttaag gaaaccaccg tttcgccggg attcaggccc tggaggaaaa
    17701 aaagacaggg atcaaatcca tctcctgtaa ccacttacaa cgaatatttt ggtctgatgg
    17761 aaatagattt cggatttcct gcagccttca gctatgactt tttcaccatt gagctggagc
    17821 cgggttatgt tattccgctg tattccctgg aaggttcatc cggtaccgga ggtttcctgt
    17881 ttatggcaag tctcttcctc aaaattttct aactttggat tatgaatgtc ctcatcgttg
    17941 aagatgaaaa aagcctggcc aatgagatcg ctattttcct taaaagggag aattttcttt
    18001 gtgacatggc ttataacggg gctgaagctt ctgaaaaaat cgcggtcaac ctgtatgatt
    18061 tcgtactgct cgatcttggc cttcctgatt atgacggcct tgatcttata agagaagcta
    18121 agaagaacaa tgcagatgtc tcctttataa taatcaccgc ccgtggtgct gttgaagaca
    18181 ggataaaagg gcttgatcta ggtgcagatg actatcttcc aaaacctttc gcactccctg
    18241 agctccatgc ccgcattatg gccgttgcca ggagaaagtt cagtatcccc tctcctgata
    18301 ttactctcgg ggaatttgta atgataatgg caggaagaaa actgatgcac gacaaccacg
    18361 aagttgagat gacaaaaaag gaattcgacc tgctttttta tctggctatc aacaggaaca
    18421 aagtccttac cagacaccag ctttacgaac atatatgggg aaatatgctt gatgatcagt
    18481 acgacagcaa ttttatcgat gttcatatca agaatatccg gaagaaactg aatgtccgcg
    18541 ctcctgctcc ctggctggaa accgtcaggg gagtgggcta caggataagc actgactatt
    18601 aatgaactca gggagtatga tgaaactccg cacaaaactt gcacttttta atctgctctc
    18661 caaagtagtg ttctccgcct tgttcatcgg tttccttcct tttatcatcg aacggataaa
    18721 tatccggcag accgacaatg agctggtaaa caaaagggaa gaagtggtca gccttatctc
    18781 ggatacaggt attgagccct ttatcgtttc tgatactgca ggaggcttcg gcagttttaa
    18841 tattttaaag gaggagtata tcagtctgga aagagttgat acctgtgaag aattgaactt
    18901 tattgacgtc accaaaagga tgattgatga agaaatcatt gattaccggg tgattcacta
    18961 ctctctcagg attgacggag aatgttacct gttggaaatt ggaaagagtt tatcaagtat
    19021 ctccaagacc ggacggaaca tacggaacat cattctggca tttctggtct tattcattct
    19081 tgtcagcttc atttctgata ttttatatac agcacgaata cttcgcccgt tgaggatcat
    19141 taccggcagg ctcggtaaga tctcctctca ctcatttgat ccatccggac cgcttattac
    19201 tacaaccgac gactttgccc ggcttgaccg gaccctcagg gaaatgatga aaaagatcga
    19261 cgaactcttc agaaaggaga aggagataac ggtaaacata tcgcacgagc tgatgactcc
    19321 tgtctcggtg atacgcggca agcttgagaa cattctcttg cggaataatc tcgatccttc
    19381 agttgaagca ggtattgaag aaacactacg aattctctac cggcttaaga ccctggtgaa
    19441 ttcaatgctg atgattgccc gcatggagag cagtcagtat ctgaaagagg agaacgtgga
    19501 actctgtgaa cttatcaggg aagtgaccgg tgaactttct cccctggcgg aagacggagg
    19561 aataagcatc agtcttcagt gtgaggggac aggtttattt ggcaaagtaa acaggtcact
    19621 gcttttttcc atgcttttca atgttttaaa caacgcggta aagcatactc cgcggggagg
    19681 aagtattgtg atcatggcag gggataacgc agggcgtttt gaggtttcca tctgtgatac
    19741 cggaagggga atgtcacgtg aacagcttga taaactcttc acacgtttca gcaacacatc
    19801 cggtcaggct actgaaagta tgggtttggg acttgcaatt accaaatcaa tagccgattt
    19861 ccataatatc gatataaaag ttatatcaga acctggcaaa gggacgcaat tttttttctc
    19921 tttcccggta aattcatgat agcttcattt ccgaggggtt atttttgatc tgtaatttca
    19981 aaataaactt taaaaatgag cataatgaga aagaaaatcc ttttcggcgt tttggcactt
    20041 cttatcagtg ccggtgttac atcacaaacc acagaaccgt tgcaggagca gacaaaaacg
    20101 aaaaaacagc tgaggacgga gacaaaacaa cagactcaaa tccgggaaaa tatccggagc
    20161 gacgatcctg acatgacaag gacaaggaca agggaacaaa taaagttaca ggatcagagc
    20221 ggtgatccga taatgacaaa ggatcagatc cgtctcagat caaaaaggca gttaagggat
    20281 caaagctgtg atccggcaat gacgcagcaa gcccgggcca ggacctcgaa tcatggacta
    20341 acggtaagca aaactgctag acagacaccc ggcggtaaag gcaagggcgg gattgtcagc
    20401 gcgcaggcga gacaagtcca caggaacatg agtgctgcca gaggtgccag gacagggcaa
    20461 ccgatcgtca ggaatgccgg acgaagataa ttgcaggaaa aaaccaggac ggttttttca
    20521 ttttcatgtc ttggcggaag gcgggacgac tgcccgggcg tattgttaat caagaatatg
    20581 ctctgtcggt ttcccgcctt ctgcgccaaa catcctgatc cttatcgtgc cgttgataat
    20641 atcgcccctc agatatgtgt tattgctgtt aacaataacc ggaaaggccg ttttccctgg
    20701 aaccggggga ttgtaggaat acctgtgcat gtgaccgctc agaacggcat caatgcgggc
    20761 ctcgttgagc accggcagca atgtctcatc aagatgcagg gtgccatgcc agttacttac
    20821 ccccgggggc atgtgcagta caacgatcct gttcccggca tccctgaatg acttgtgctg
    20881 aacagcctgt ttaagccatt ccgcctgctc atccctgtaa gcatcaaaag cagcaagacc
    20941 gctgtattca atgtcggaat cgggtttgtc ctctccacaa tccagtatga tgaatgcaac
    21001 ctttccaaca tggacaatcc tgtatatttt cccgtctttg accgggaaat agttcatgag
    21061 aaaatcggca tatggcccgc gggtttcatg atttcccctg gtaaatacta tcggcacccg
    21121 ttttgcaaac aggtcggctg cagcatcaag gaaatcggag aatatttgtt cctcactgat
    21181 gatccaggtt gacatatccc cgttgaagat caccaggtca atcgaactga aatcaatgtc
    21241 cctgcaaaga tccttcatga attctgccct tccgtggata tcgttcagca tgatgaatga
    21301 cacagtatca tcgttagcac tgaatgtttt aaatgacagg gagttccagc tgtatgcagg
    21361 gttggcgatg gtagtgccga aaatgatttt tgcatcattc tcccagtgca ggacttcctt
    21421 tgagaagact gcataacggt acctggtacc ggggctgaga ttctctatcc tgaccttatg
    21481 gagattcgtg gcagcctgtt tgcggcccat atgcgtatcg tagtatttcg gacgttcctt
    21541 tccgtagaaa tgacttccgt catcaggggc aacttcgacc catgaaaggg agttcttatt
    21601 tgtcatccat actattgtga ctcctgtttc agtcatgtcg caaagccagg gcccgtgagt
    21661 tatcctgaaa ggcagctcct ggccgtatgc aagaaggcta actgataaca gcaccggtaa
    21721 aaaaagcaag cttcttttca tgtcttatta ttattattat tcgttttcag actcaggatg
    21781 gaattaatgg tttccagaat tgaggattct gtcggggtat taagttatct gaaatggtaa
    21841 gcaacataac tgacccgata agaaatacaa gctaccgcgt tttcagttca agggttacca
    21901 ggaccggcct atgatccgaa gcagaggcgg gaatgacttc tacatccctg actgtccagt
    21961 catgaccacc cgataggaaa ataaaatcta ttctcagaat ttcaggaggg tatgtgatct
    22021 gggggttccc tttgattaaa gctgcatccc tgaatccgcc gttgattacc ttatagggtt
    22081 cgctgtccgg tgtaaaattg aaatccccgc ctactatcac cggcagcttt gttccctcaa
    22141 gaatttcgcg gacagctttc gcctgtgcaa gtcggttttc agcatactga tggcataaat
    22201 gggtgcctgc aaaaattatt ttctgcccgt ttgggaatgt atggctaacc agcagcaaaa
    22261 cccttccttc tccacctttt gggttcggga ggctgtaatt tgaagattcc gcgggaaagc
    22321 gtgtcagtaa tccttccccg taaccaccgt tgctgtaatc aatggccttt ccgaagaatc
    22381 cgtgcattcc ggtcatccgg gcaagttcgg caaccagatc catggggatg tcattattca
    22441 attttgccga acgcatcgtc atgctgtcca cttcctggag agccacgaag tcgggcttgt
    22501 acttattgat cacctccgca accattgcca ggttgctctt gccgggatta aaatgctgct
    22561 ctccatggta gatattatat gtcattaccc tgatctgctg agcaggcaat ggcagattaa
    22621 aagacagaaa aagcagcatt gcaaggaaag catggcagaa tgatctgaac ctgtcggatg
    22681 gtctcaacgt accaggggaa ttataatatt tgttcatatc agtttttgta tgtttatatt
    22741 actttgaagc taaaataaat aaaaaaacaa ttcagtgtga atccgggatt tcccgatatt
    22801 accggatacc atgcagctcc cgacggcttt ttgctttgac ttgcacagac tgcttccggt
    22861 catggtttca aaggtatgaa tagccatggc ggaatgtgga taatgatgat ttcagccgtg
    22921 aaagtatcat ttccaggcag cttcacctgt tgaatgtccg gaccttgtaa aggtcgatat
    22981 ccggaatggt gatgacttta tccggattaa gacaatagat atggtgtgtc caccagctta
    23041 tttcagcttt tgtgtaaata agctgcccgg tggcaggatt aagattgaca gatttgatat
    23101 cagccccgtc ttcaagcatt tcgaaagctg taaatttctc ttttccaata tcaaagcgcc
    23161 aaacgctttt tgatgttgta acgagaagtt ccgaggccga aacagcggta agatcatgtc
    23221 ctccgtcgtc aggggtgcgc cattcccgca taagctgcaa cccgggcgaa ccgatatcgt
    23281 ctgacacgag cgaatattcc cgtagttcat tgaaacccag tgcaaaaaga cgctgaattg
    23341 ccgggatcca gaccactccg tgcccggaat acaaactgtc cctgaaaaga acaatatcgg
    23401 acttttttat atcatataat tccaggctgt tcccgtcttc atgggtcgag agggcaacag
    23461 ctatcaaccc tcccggaaga tattcggctg aatgtgccat cgggaccttt gcgctgaaca
    23521 gtattttttt tgtctgcctg tcaatcagta caactcctcc gccggaggag gttaccagta
    23581 tcttttttcc tccatccaca ggtttgcaat cgtctgtagg aaccataagt ttcttcattc
    23641 cggaagaaag atctgccgca tctccgacgg tccattccca gactacttta acattttcac
    23701 cggtcgaatt atcaaggtca attatcagaa ccttgtcatc cccgcaggcg attatttcct
    23761 ggctgtcccg gtgtttacag gagtatcctg tcatgatcag gataatcgtt acgacggcta
    23821 aacgcatata tgttctcatc tgtaaatgat ttggattgta aagaaagtca tgatttcata
    23881 tggttgtaaa cgagcagatc ccatacgtat ttgtgcttgt ggtcaatcat acttgtttca
    23941 ttttgtcggt aattatccag tgttcctgag tcagtccggt attgatgctg aactggataa
    24001 acccgggtat tgttaattaa ataacactgg ccagctgaat tgttaccaat ttccggcgcg
    24061 tgcataatat ccgcacaggt ttgtcatgta aataaacaaa aatatgaaga atgcaagaaa
    24121 tctcaccttg cggatttcaa tgacaagcaa acaatcaatg aacattcagg ttaaaaagaa
    24181 atccaaaacc ttgacggcat ttaatgatct aagcatatct ttggaatttg atacggaaag
    24241 gattaatgtt gttttaattt gttgctatgt cgcgtaaacg aactgttgaa gaaataaatg
    24301 agaagatccg caaaggtaag gccgtagttc tcactgcaga gcaggttgcg gagatgggtc
    24361 gcgaaacagg atcagccgga gtggctgaaa aggttgatgt tgttaccacc ggaacgttcg
    24421 ggccgatgtg ctcatctgga atgtttatta acttcggtca tacagatccg cctatccgca
    24481 tggagaaggt tacgctgaac aatgttcccg tctttgcggg tatagctgcc gttgacgcct
    24541 atatcggagc cacggaaact gcgttgcccc acgatcagta cggcggggcc catgtcatcg
    24601 aggaacttat tgccggaaag gatgtattgc ttcaggccac ggccaaggga accgattgtt
    24661 atccgcgtac cgaggtcaag acgcttatca gcagggactc ggtcaacgag atgataatgt
    24721 tcaatccccg caatgcctac cagaactata atgcagcggt gaacagcacc ctgaaaatga
    24781 aatataccta tatgggcacc ctgctgccct cattcggcaa tgccactttc tccacatcgg
    24841 gcgaactcag ccctctcctt aacgatcctg aaatgagaac cattggtatc ggtacccgca
    24901 ttttcctggg gggcgccacc ggatacgttg catggcacgg cactcagttc aacacaggaa
    24961 aacccctcaa cgaacacgga gtgcctgtcg gaaatgcagc cacgctggcc cttattggcg
    25021 atgcaaaaat gatgagcact cagttcatca gggcagcata tttcgaaaag tacggggtct
    25081 ccatttatat aggcgtgggt attccaatac cggtgcttga tgaggatatg gcccggcagg
    25141 taatggtgcg caatagccag attgagacca atatagtcga ttacggctcc ccaggccacg
    25201 aaatactcgg gcgaaccgat taccattcgc tgttctcggg tgagataagc ctcaacggga
    25261 aaaaaatacg cactgccccg ctgtcaagcc tgaaaattgc ccgcgagata gctgaaagac
    25321 ttcgaagtga gatcttatcg ggaaatttct tcctgacagc cccggttgag ggcttcagaa
    25381 agacccgggg attgaatagt ctgaaaataa ggctttaaca aaaaataaat ttacgatcca
    25441 gatgaagagc agaagatttg tgctgacctt tcctcctgaa acaacaggtg agccgataac
    25501 ttacaacctg atcaggaaat tcgatataat ggtcaacata gtgaaggctg atgtgacacc
    25561 gggccatata ggccacctgg tgatggaaat gaccgctccg cccggcatcc tgaatgaagg
    25621 acttgaatat atcaggggtc aaaacgtgga atgtgttccc attgaccgca ggatcaccta
    25681 ccggaaagat atgtgcattc actgcggtgc ctgttcggca gtttgttttg caggagcgct
    25741 taccatggac cggacaacgg cagaacttac atttgaaccc gaaaagtgtg tggtttgcga
    25801 gctatgcctc acagcctgcc ccctgaagct tttttccatc gatcttggct aaggtccatg
    25861 cataccagcc gcgcctttac cgggaaacta tgggtaacag gcgctggatg agcttcaggg
    25921 ctgcctggcg tgaaacggac ctgtgggtgg caattgacag ggagcattat cagcacgaag
    25981 ccaaaagatt taccctggat agaatagtat actaccgtga tatcctggaa aactatattt
    26041 cactccgacc cgagttccgt gactcgctca ccccggtatc ggtgcccgac gggtcacatc
    26101 cgctaattat ctcaatgagc aacgccgccc ggcaggcagg aacaggcccc ttgagcgcag
    26161 ttgccggagc aaccgcggaa tatatatgcg gggacatcat tgagaaattc agggcagaag
    26221 aggtgatcat agagaacgga ggcgacatct tcatgaaatt aactgctccg gttaccgtgg
    26281 ccgtatacgc cggcgattcc cccctgtcgg aaaagatcgc actgcggccg gcaccctgca
    26341 acactcccct tgccgtctgc tgctcttctg gaacggtagg ccactcgctc agcttcgggg
    26401 tggctgatgc atgtgtgatt gcctgtcgtg acggcgcact ggctgacgct ttcgcaactg
    26461 ccttcggaaa tgaagttaaa ggaaaggaac agctgggcgc ggtgacggaa aaggcactga
    26521 aagtgcctga gatcatttca gttgtaatag ttaaggatga ccaggttgca atgggcggct
    26581 cacttgaaat aaaactggta tgaaggagac ccggtaaagg gatactgcct aacttccttt
    26641 atgactaatg aattcatctc agtatcagtt tcatgggaac cctaatgaaa cgcgcccggt
    26701 atttttcaac atcctcgaaa atatcaatcg gatttaatga gtttacacaa tatgaaacca
    26761 ggagcatgcc gttttcaatg tactgcctgt gagcaagagc attatacgta attacacgtt
    26821 tgttcggatc agactccatt tccgttgtgt ggtaaatcat tttcttgtta tggaagcccc
    26881 tgtaaggaga atccgatatg taggtataaa tatcggcacc ggccctttcc tgggtcagca
    26941 gaatgtattt cctgccgtat ctgaatatgc tgaattgttc tgagacggaa atgtcgatat
    27001 cgagaggcat tgatttcctg atgtccttat cccacgatga acctgtatag tattcccagt
    27061 ctgatttaaa aatgttactg ctgaagggaa cgcgggccac atgtattttt gacatgtccg
    27121 gcaggttcct ggtgccatat atgtatatcc atcctttatc caccatcacg cagttaccga
    27181 aatcgatcag gctggaggga taatcaatat ttacggtttg agtttcaata tgcctgaaat
    27241 cgggataact gaatgacagc agatcaatat gggatatccg gaaagagaat atctcctctg
    27301 ccattctgcc tgtttcatca tccgcacctt caggaagcct gatgtttatt acaaccgaag
    27361 tgtcatttac caggctgagg gcaaatatat aaacagtgtc atttttgata aatccgtggc
    27421 ccggccagta ccagcggtat gtggaatctc ccggctcatt tacaggttcc atgaaagtat
    27481 ggggatcatc agtgcttccc ctgactattg cccgggtacc tgtcagttcc ctgtttacaa
    27541 taataaaggc atttcgcagc atgggtgttc tcatatcacg tgtcccgttc actactttcc
    27601 cgaggaagca atcgcccagg aggaaaacgg atgatctatc cggtaaaagg aaggagaaaa
    27661 gaccatccgc accggagatc ccgcctgaat cggaatagaa taaggagccg aattccttat
    27721 cagggattac agggtacgat gtaggatcag caccggattt gcatgactgg tgcgccagca
    27781 tagaaaatag aaatacgacg gctgtgaacc tgacgagatc attcctgtta actttcattt
    27841 gtacattgct ttatctgtat tgatcttttc ttctgttttt tcatgcggtc cgtatctgac
    27901 ggtaatgaac cgcgggtgat aaatagtggc atccttctga atgtcttccc agaaattcag
    27961 tgtgatcgtg ttgtaactga ccagcagttc accgggtttt gaaagatgat aatgagcctt
    28021 ggcattataa ggcagcaaac ctttttcctg gtattcggga catgtatata cttcattgat
    28081 ttctccgaag ggcccggccg gaccagagcc aaccctgatg cctattttat ccgatatacc
    28141 cattacggta aatgtgagca ggtacctgcc gtcatcggcg ggtgtgacac tgagttcgtt
    28201 ggaaacagcg tcggtaatgg ggaagatatt ctcttttcct gcgacccatg cctctccgtt
    28261 ccagaaagtc catcggcctg tctcctcaaa gtgttccggt ctgacccgtg cggcaaccag
    28321 tgattttttc tcccctgcaa ttccgtatat gtaaacatag ccatcggggc agggagcgcc
    28381 ggctttcccg gtattgacaa aaattccgct gccaaagtat acccttccgt gaacaggatg
    28441 tacaaatccg agttcggtgg gtatctgctc acagataccc agacccttcg gggtgggctc
    28501 cctgattttc aggatggcca cattggtggc ctcaaaatca aaaacattag gaccagtttt
    28561 atgaacatga taggcgaaaa tgtaaaggga attctttttt gcatgattga caaatccatc
    28621 cccaagccag aaatactccc cgtcacggct gttcctgttg tcgggaatga aatatgacac
    28681 agggttcccc tcattatcct tgttgtaacg gaaaacaatt actgattcat cgggtatgac
    28741 tccggtcatc catgctgcgg tgttatttac catgacattg ccaggcaggg gcacattgtt
    28801 ccttacttcg ccaatgtatg tatcactgaa gataaagagg gtccgtttgt tcttgttttg
    28861 ctgatcttct acaccatcaa gcgggatgga aaaaataccg tcagccgcga accagccgga
    28921 agtccgctcc atcagggcgg tccattcagg agccggttcg cttgtaaagc aaaggggatc
    28981 catgggccct gcctgaccgc aggaagcgcc ggggccgcat gaagtgcagc agccggccag
    29041 cactgcagct gatgtgacaa tgaaggctgt ggttttcatg attaattcct gctcaaagtc
    29101 attggtttca gtttattcct caacgtactt tattattgca tccttccata acagataacc
    29161 cctgccgttc agatgcagcc cgtcaatgct gtattgcggg ttcagcttgt gattttccgt
    29221 ctggaaaatg ttgaaaagat caatatatgt caatccgttt tcagtcgcaa tatccttaag
    29281 atgatcattg atcctgatta tttccgtgtt ccggcgtgta tagtggatac cgtcatccgt
    29341 gggcagtaca ctctggatat agatcctcgt acccggggac gattgcctta tgcgttcgat
    29401 tattctcctg tagttcccaa caatgtattc aacacttttc ccgttcgaca ggtcattggt
    29461 tccgatcatg atgaatattt tggacgggtt ggattcagtg acttcatcaa gacgttcgag
    29521 gatgccgtcg gtatcgtcgc cgcctatacc cctgttcttg atgttgggat tactgaaaag
    29581 ctcccaccat tcgcagttgt cagtgatgct gtttcccagg aatataatct cgtttggggt
    29641 gtttggcagg ctctcaaaat gttctttttt gtggtagtag tatgccctga gtgcctggtt
    29701 ccaggctgcc aggtcctcgg catatttatc gtaggccata attgttttag tttcctccgg
    29761 ggtcagccgg gtgctcatag agggtggtga gtcggcggga ttgctccccc atttcaggtt
    29821 tggtaccggc cccattacaa gttcaagcat gccccctgcg gccaggtctg cctgcctgaa
    29881 ccatggcttg ttcaggacgg tgcggtttag cgaggccctg cggatatatt tgttttcggc
    29941 agaattattc actgttttga ttacaaattc atttccgcga tagtatttat tatccagacg
    30001 gatggttatt ttctcaaaaa gtggactgcc aatctcataa aaaggctcgg cagaggtacc
    30061 gccatcggtc tgaaaaagcc ctatggcgct cataacaaat aatgctcctg cctgggcggg
    30121 gtcaatgcac ggattgaatg ctcctgcctt tcctgacctg tattccgtca tgatatcacg
    30181 tgtcagtttc tgggtcagcc atggctgacc ggtataattg aaaagccatg cagcctgcag
    30241 aggatgttgt tccgttctgt aatcgcccga atacaggaaa gcttttatca ggtcgggact
    30301 ggtcataccg gagcagccgg acttcaggga actatcgatg ctttcttcca gctttcggat
    30361 gaagttttcg cggccggaag agatcagcag tccattaaca tcatgcggga caaaagcaga
    30421 caagggcttg ttctcccggg gaggtacatt tttataattg aaagctcttt ttgtaaatgt
    30481 cctgaagtcc ggaatattac ctgatctttt cgccatttgt gcagctgtcc agtcagtgaa
    30541 ggaatatccg caggtaatgg cagctgagtc ggcggttcct cctgcaatac ccgttttaag
    30601 ataaggttcg agatacacta caccggcaaa tcccccgccc cggtgaggag ctcccgcgac
    30661 actcaggtca tggacgacag ccttgtacat catgcttgtg tcatagttgc ggataccctg
    30721 ttggtaggtg cttgccatca tcgggatccc gtatgaaacc accggcgttg ccggatattc
    30781 cactccctcc gcagcttcag gcagccatcc tcctttctga ccggtttcca gcaatgaaag
    30841 cacacacttg ttggacagat caggctggca cagattcctg agctggaaaa gggcaatggc
    30901 ccctgcaccc ggagggtcac atccgtaaac aggataatca gggttgccag ggacctgcac
    30961 tttctcatcc atgtcaacat acatgccgtt aacatcgctc catgtggttt gtccggcaaa
    31021 cgcccgatac aatgaggtat agaacagctt caggttctct tcatcaccac cttccaccaa
    31081 tatggttttc agaagcctgt tccattcatc cctgcttttt tccctgactg aagtgaagtc
    31141 ccatgcaaac ggccctagct cggtttcaag gttaagcctg gcctgatcca cactcacaag
    31201 tgacaggccc gactgaacaa gtatctcttc gccatctttg gtactgaaat caagaaatac
    31261 gccaattccc ttgtcttcat acgacaggtt gatctcttcc gcattgcgga ggatcctgtc
    31321 tccaatccat ccattgaatg atttaaaagg tttgctgagg cgaatcacaa aatgaattgt
    31381 atatctgttg gcttctgcac ccatcaggct ttgctggatt gaaaaaccgg ctatctccgt
    31441 gctgcttact ttcctgatac cggctccggt aatctcatac ccatggctgg tagggatctt
    31501 tagatcgatg agcagccggg cagtgtcgct ttcaggatag gtatacttct ggaatgcagc
    31561 ccttgtggtt gatgttagtt cggcttttat attataatca tccagaatga ccgaataata
    31621 gcctggttgt gctttttcgt tccggtgact gaatcttgag cggtaaccgg aatccggatc
    31681 ctccgggctt ccccggaaga tattaagtct gcctgtaaca ggcatgacaa gaagccctcc
    31741 catggtcctt gaatggatat gacagaagcc ggctatactt tctatattat aatcgtatcc
    31801 tgctttccag ccggctaact ggttatccgg actgattttc accattcctg atggcgtgga
    31861 tgctcccggg aaaagcgtcc attgcggaga ggaagtaccc atgagcggat caacatagtc
    31921 aaccggctcc attgattgtt tctgtgctga aagtactgca gccgatgaaa tcaaagcgaa
    31981 cagcaggata tattttttca tctgaaaata aatacttata caactgaaga ttaagtggtt
    32041 cacatggaac ccgcatgaat tttattttca tgcttgtttg tgattttgta tcatgtgggt
    32101 ttcatatgat ttgatttctt taagacgttt cagtgctgca aatccggaga agtcagacct
    32161 gcacatgaga tacgtgaaaa ggcatttttt atcttaacag aatcttcctg gtttcaacac
    32221 ccctgtccca tttcaggcgt gcaatcacca gtgtaacagg gaagcggctg caatctattt
    32281 cggtatcggc tttgatcatg cgagacaggt acagtcttcc gtcaagaccg taaatactta
    32341 gcattgaagg ggtattgacg ttccgtatat gaattattcc tgttgcaggg tgataaataa
    32401 ttctggcatg agtattatcg tctggcacgg ggtcgacagc aaccggggta ttgaaaagct
    32461 taatggtatc aggttttgtc actgcaactg cagcttccgg atcgagccag aagaaacggc
    32521 cccggtaatt cctcgtatca ctgtaaatag aattgaagtt tgtggtgttt acattgaagg
    32581 aaatgaggat ttcgccgttt tcccggaact gcggatgggc aaacagatta taggttatat
    32641 ttgtctttgg cttggtaata accgcaaacc ggttttcaat gccaccggca gccctggtgt
    32701 tccagggtcc ggtgattttg tcggcttcaa ggatccatag ctcgccaaca agccatattt
    32761 catgcatcaa caggtaaaat ctgccgtttc tttcatatac atagaatgat tcactcatcg
    32821 gccggttccc gacaggtacg gcatcggcga tgttacgtga ccaggtgtcg ggccctgcat
    32881 aaaactccca cggttcttca atggtttccg atacccgggc gaggtaagga agcggatagg
    32941 tgtattgccc cgacgttgta tctttcaccc cgaaaatata ggtatgatca ccgcttttaa
    33001 tgacgcaggc gccgaacctg aaagaagagt cagtcacgga tttgaacgga gttgttgaga
    33061 tgtattcaaa accgggatac ctgaacgagg ccttgtatgt tgttcccaca atgaaattga
    33121 agcctggtac cccggtattc ccgggtttta tcctgacagc aaagatctta accgtatcat
    33181 tttcaatgac agcatgctca ggccagaaaa tgtcttttcc ctctccggga atgaacgacg
    33241 acgggtttgc aggagtgcct gcatataagg ttttcatttc atttcccgtc tcaagtatgg
    33301 ctgagttatt tatgaatcgg gatttccccg gattgatgct gtattccccg gtcctttctc
    33361 caataaaact gtcaccgaac agccagaggg tgttattgtc gggcagggga agggaaatgg
    33421 ttgcatcggc tgctaaccag cctcccggca cattgttgaa tttcaagaaa tgctccatag
    33481 cttcccggac cacttcctgt gccgggatac tgtaaggcac aacagcaagg ccgaatatga
    33541 atccgggaat taaaagctgt ttaacagggt aaagaaatag tttcagtttg ctgagcacgg
    33601 tatacattat taattatatt attgcttccg gttcctctcc ctgatcataa ccggttttcc
    33661 cgatttgtat tcaatggtat agtattcatt cctgtaatat acatgatgca tgcgtccgtc
    33721 gaaagtgaag tccttgggtg gtttgatcac cgtaccgttg agatcgggat agaatccgaa
    33781 gaagtccttg agcatggtct gggcaaatgc gacacctgac gatgattccc tgttgcacca
    33841 gtcacgttcg gctattctga ccaatgcctt tttgttggtt ttattttctc cccataattc
    33901 atgggcctgg gcccatattc cttcatttgt gacaggctca acagcgtgat agaagttaag
    33961 tgccttttcc ggataaccca tttgtgtgag agcatccata atatccaccg gccaaccgtc
    34021 gaacgcaccg aacggcccgt gatcagcccg gtccgatttg tcggcggcag gatcactgag
    34081 tgactgtgcc ctcatccaat gatccgtgat cagttcgtta tacaggaatt caagcatctc
    34141 gctctttatg gcggcaggaa tatcatcggg gatgtatttg ccgaaaaagg caaaatcaag
    34201 gcaatgcctg acctggatcg ttttgttccc cgggtaaagg ctgttccaga caccgttgcc
    34261 tgcataaagc ttaagaatgc gcccgatcat ttccgtagcc agggtattca gtgaatctgc
    34321 tttctgccgt tcgccccgat gcagatggag cttggcagcc tcacgcatca tccaggcata
    34381 gccgatattg aatgacggca ctatatgctt gtaggtagga acacattcga gaaggttcca
    34441 ttcatcgtca ccgaaatcgg caagtttgta gatatcatct gtgcaaccct cagtaccata
    34501 gagggagatc ctttcccagt tcattgcgta gtcatcaaga tgttgcagaa cggtcccgtt
    34561 accgatggtt tcatcaagaa aggcataatc accggtgacg gcaatatagt cgcggatcat
    34621 ctggaacagg gcccagtaat ttgcagaata tccatttccc acacccttgc ctccaaaatt
    34681 atccattccg taatatttgc ttggatcgat caatatccat gaggatatgt gctccttcat
    34741 catcacggga tcaacgactg cccatagtgt tgaccacaat gtgatatccc agaaaaaggt
    34801 gatggatgcg ccccagcgcg gcccgcccgt gagaaacacc ttactgtgct gcggaagatt
    34861 ggtatttgtc atgtaaagca tcgtcagggg acttgtataa taaactttcc gggccgacag
    34921 atcgccagtt tccaggacgg gaaagcatcc tgaaataaat gaattgcccg gttggaaaat
    34981 ggatttccat ttttcttccc agctgtcctt gattcccctg aaatagcctt caaaaccggc
    35041 agaccattcc agaacatttt gttttattat ttcatggttg tctccgtaag acatgaggta
    35101 cctgatcttt cgggttgcgc cgggctccat cctgaatttc catgacactg tggcgccgca
    35161 tttcttcggg gagatctgat caggagcggt aacaactgaa aatgaagtta ttgcttctga
    35221 ttctgtgtcc cgaatataaa tgacgttgtt ttcaactgag gttgtgaatt tagcgctgtt
    35281 cattatttcc tcatcgctcg gccatttcat cagaaccttc tgaagcatcg gcttgccgtt
    35341 gaccagctcc catgacagac cctctttttc aaatacctgt tcaccaaggt gctccctgat
    35401 attttcaact tcctcattac ggtttgtgac cgtgcccgac atgcttggat acggatacca
    35461 ccattgccag tctccgccga atttgctgac aaatccgata agatcaagtt cgggactgat
    35521 ttcccgtgcg atggtgtcct tattggttat ctccacttcc cacataattg catccttatc
    35581 aaaaagcatc cgcgtagagc tgaggatctc aagattgtcg tgggaaccct tccttagtcc
    35641 ctggtaaggc tgccagcgcc atgagtttac tttcggtgtc ttgccgttta tcctcagggt
    35701 tccgctgtgg tatccgccgg agaatggcgc ggaaacaaac cagcttatcg atgtcatgtc
    35761 cctgttcagc agagcctgtc cgcgaaagtt tcttatggaa ggctccattt caacggtgtc
    35821 ggttgatacc cagaatgctg acagttcgtc gagcgacggt atggtattgt acggtgaaga
    35881 ctgatcattt gtatgacttt tgcaagttgc aaggatcatt accaccggaa ggaccatgat
    35941 taggatcggt ttcttcatgt cggtcgttaa ttaaggttta tattttttgc aaacattcct
    36001 atttcagttc cgcctgggag gccggttttc cgggatcctg ttcagtacgg agcagtaatg
    36061 tcgcggccgg agatttttcc cgccgttgta ataccgttgc cggatcctgc catgtgaatt
    36121 gaaaccaggt tgaactgctg tcatgatttt taattataac cgttattctg agtaaggttc
    36181 gggttaaggt acctctcttt ctggggtatc gggaaaagga tatgatgtgg ctgtatgttg
    36241 gctgtgggct tagctatgcg gatacgttca aacagctttc ccatccttac caggtcgaac
    36301 catctgtgac cctccatgac aaattcccat tgtctttcct gcagcagggc atccttgaaa
    36361 gcctggtaat ccagtccggc ggcaaggtca tccaatccgg ccctgttgcg gattgcattt
    36421 atggaattat aggcttcagc agtgggcccg ttattaattt cgttaaggac ctccgcatac
    36481 atcagaagca cgtcggaata gcgaagataa ataaagtcgg catcagactc cccgttctgt
    36541 cttcctcctt ctgcttcctg gtcccagtac ttcctgatat agggtccgtt gaaaatctca
    36601 ccttcaaatt ctgttatgaa agttgctgcc tttcttttat cgcctggtgc gaagctgttg
    36661 taaagtatgt ccagcgggcg ctcccatcct tgtccgttgc gggttccgtt tgccgcgacc
    36721 gtggtgggca tgaggcgcac aatgaactgg tttgttgtcc agccttcacc cagtgttccg
    36781 ctgaaattga cggcccagat tatctcaccg gcattagcat ttttcagctt gaaaatgtca
    36841 gcatactcgg gttcaagagt gtaattcccg ataacctttt caaggctggt tttggcaaga
    36901 ttgaaatcac cggcattatt ggtcagtgca ccccgggtca ggtaaacctt tgccagcagg
    36961 gctgatgcag caaggctggt gggcattccc tgaaccctgt tgggaaaagg gttgatcctt
    37021 agcagctgtt ctgctttctc aagatcactt ataatgcttt catagacttc tgccaccggg
    37081 gtcctgggaa tctgggtctc tgcagctgtt ttggttgaaa agagctgcag cgggatatcg
    37141 ccgaacagcc tgacaagatc aaaataaagc attccccgga gaaaatatgc ctctccccgg
    37201 aggtgatcct ttatctccgg cctggaataa tgttggatat tctctgactc ctcgagcaaa
    37261 acattggttg agtttataac ttcatagcag cccacccaga tataatatac cgtagcatta
    37321 ttctcgtcga aggtaaattg tgaaaggtca agcatgtcga tggaaggccc ggtttcatag
    37381 caattgtcgg atgcaaggtc ctgcaatacc caatagttat taaagaacaa accacctccg
    37441 gtccaggtcc aggtgatcag accaccgaac atgccgtcag caaagcttac caggtccttt
    37501 tctgcgagaa aataattgtc atagttcagt tccgaacgca ggtcctgctc aagaaacccc
    37561 ttgcagcttc caagtgaaag cagtactgta agtattataa tgattttttt catcataata
    37621 taattttgat tttaatggta tgatgcccgc ctgacccgtc gggcagggaa ccgcacatga
    37681 ttgactttgt cgagctcgca agctcggttg aaaattatat tcttatcgtg tttgtgattg
    37741 tggtcaatca tgtgggtttc atcagtatat aatttatttt taagaggccg atacctttct
    37801 gacccccggt caggaaacca tacatggttg aacgcaatta tattcattcg tgtttgtgtt
    37861 tttggtcaat caagtggatt tcatggtgct gcctggtatg ttaaaaagtt gcatttatgc
    37921 tgaacaaaag ggaccttgca gtagggtatg cgccgtaatc cgctcccttt ccgaagacac
    37981 ttccgccgaa gattgacact tccggatcgt atcctgaata ccgggtcagt gtgtagaggt
    38041 tgtaaccgct gagcgacagc tggatattgc gtgttcttat gctgttcagc agcttctgcg
    38101 gcagggtgta ggagagggac acctgtttga gcctgacata ggaaccatct tctacataca
    38161 catcggacat gatggttcca tgtggcgatg cattggccct gggatacttg ttcgtcggat
    38221 tggtctcggt ccacctctgc aatgccactg cagagttgtt ctggaaaccg tcaaagcttt
    38281 caagctggaa cctgttgaaa ttgacaatct cgttgccata atcaccctga aggtagatat
    38341 caagcgagaa attcttgtat gagaaggtat tcctcagtcc aaaggaaaaa tcagggttgg
    38401 cgtttcccag cttaaaatag tcatccactg taagcttgcc atcattgttc ctgtcgatgt
    38461 atttcctgtc gccgtacctg gcaatcttgg aagggaagaa gggaatttga gacaattctt
    38521 cgttacgctg gatgatcccg ttcgatttgt agccataaat aacacctatg ggttcccctt
    38581 cagtgatcat tgtccagtca aggatattca atacggaaac cccatagatc ctgtctgact
    38641 tatcgaggtt cgtcactttg ttccggttaa acgagatatt aaaacgggta ttccaacgaa
    38701 agtccccctc gaggttgacg gtgttcattg aaaactcgac acccttgttc tgcaggtcgc
    38761 caacgttggc ccagacatat tcaaatcccg atgacctgtt gacgggcagg aacagcagca
    38821 ggtcggaggt atttttcagg tatgcttccg ctgtgaattc caggcgtgat ttcagaaggc
    38881 tgaggtctat gcctgcatca tactgcctgg taacttccca tttcagatcg ttgttcctga
    38941 gtgaataaac gtatgtacca ataacttcag cgttcgatcc ttccccgtag aagtagggat
    39001 gagtccccat aaggcttaat gttgcaccgg aagggatacc ctcattgccg acgcttccgg
    39061 cactaagccg gagtttcaga tcattaatgg ttttaacacc ggagaaaaaa ttctcttttg
    39121 agatacgcca tgcagccgat actgacggga aaaatcccca tttgttttcc gttccgaact
    39181 ttgacgaacc gtcgagcctg cccgacacag taaggtagta tttgctgtcg aagtcataca
    39241 ttaccctgcc aagtcccgat aacatagccc aggagctgta tcccgacata atggttttgt
    39301 caagccccat acccagattg taatatgtaa gtcgttcatc ctcgaagcgc tcaacggcta
    39361 tctctgctct ctcagagaca aacttctggg cggtctggcc aagaagcaca ttgaaatgat
    39421 gccggtcagc tgcaaacttg taggtcagcg tgttctccat cagccatgta tagcctttga
    39481 tatcgacatt cgaccccttt cccttgtcct ggccgcgttt aataaagcta ggtatgtatt
    39541 gctgttcctt gttgaagaag gcgtctgcac cgaccttgaa cttgaactgc agatttttaa
    39601 taatatccca gtcaacataa taatcgccaa ttagcctgtc agtggttatt acaatgtctg
    39661 tttcatttat ctccgcaacc gggttcggga tattattgat ggtattgctc tcgtatgtat
    39721 aacctcccac tcttgtggag tcataaactt cctgtccggg attgaacaaa agagccattg
    39781 cagtcacgct ggaagccacg ttgggtaccc ctgtagtgac accgtggctg acggactgcg
    39841 acatgttcag gtttatgcct gtcctgacct tttctgacag gtcagtatcg agattgaacc
    39901 tgacatttcc cttgttgaaa tcggaattat gaatgatccc cttattagtt accaggttgg
    39961 cagaaaggaa gtatttgatg tttcccgctc cgccctgtaa agagatttgg gaattggtca
    40021 gcggggcagt gcggaaaatc tgatcctgcc agtcggtgcc tttcccgaaa cggcttggga
    40081 ccgcaaagtt gcttccatag gcgatttcag gataatattt ccgggcttcc gtgacagcat
    40141 cattcccgag ttccgccagt tcccttgcat taaggagacg gattttcttt gccagctgct
    40201 ggtagccgta tgaaaggtta aggttcacct gaaaatctcc tttttttccc ctcttggtat
    40261 ttatgaggat gaccccattg gctcccctgg c
//
LOCUS       DAOOYG010000012        38948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_874_length_38948_cov_6.702363, whole genome shotgun sequence.
ACCESSION   DAOOYG010000012 DAOOYG010000000
VERSION     DAOOYG010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38948
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_874_length_38948_cov_6.702363"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /gene="pnuC"
                     /locus_tag="PK288_02225"
     CDS             <1..344
                     /gene="pnuC"
                     /locus_tag="PK288_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HPM17762.1"
                     /translation="PEIRRIDRKTGAWCLTSAVALWVLLWFLLDKATGSPVALWDGLI
                     ASLSVVATWMLTRKYFELWFVWLAANAIAVVVYLSSQLYPTGLLFLVYFSMSVVGAKE
                     WRRVMTAGSKY"
     gene            371..988
                     /locus_tag="PK288_02230"
     CDS             371..988
                     /locus_tag="PK288_02230"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPM17763.1"
                     /translation="MNIVILANGNYPSHEVPLGYLGRADLIVCCDGAVEKLVTNGLEP
                     GIIIGDLDSVTPALKRKYAGIIVQDLDQDTNDLTKAVNWCVDRNYDNLVILGATGNRE
                     DHTIGNISLLAEYVKKTTVKMITDTGIITPHLTSSRFSSFRGQQISIFSVDPETGITS
                     VGLKYKLENMKLRNWWQATLNEAEGNTFDLIFEGGPLIVFQKFRK"
     gene            complement(1023..1955)
                     /locus_tag="PK288_02235"
     CDS             complement(1023..1955)
                     /locus_tag="PK288_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM17764.1"
                     /translation="MFEYLGYLIFILSVALSAGGIILSSRLRSRNGAGIYSTLLYYQV
                     FIYAFGFYGIWGQVVARMFLSSLIPPDILIRVNNITTLLGLPFLIFAWLMLIRFSGIL
                     SGRKSNNWFIAGFLLSNFIILAVLGYFFMTRLNSRNTVLIIRNYYILMNLVHTFIAAY
                     LIHLPVKGKSLIKEHDRKVIAPAIFLIMIIQCIPLVLIDLSPWVAIIFIFTFFLGNIF
                     LPVYFSYLIISPVIIDRTNNFLTLEQFCKRFDISPRESEVVREICSGLSNREISDKLF
                     ISLQTVKDHTHRIYIKTNVKSRVQLINLVNDMVK"
     gene            2265..3017
                     /locus_tag="PK288_02240"
     CDS             2265..3017
                     /locus_tag="PK288_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17765.1"
                     /translation="METEKNYNLIPTYGNSFRTGWKVMFDNFLRLLLVVFVLALLTAP
                     LKMFELKLEPSDFHGQLDKWSDIWEYDMGKIVTLGTLGMVAAFAGLLALAYTFLAIPV
                     VSYGGNMIFVQAVRRIKPEFDLLIRGFRENYLHIVLSNLLLFALIVLGCFALIIPGII
                     IACRLVFVPYIVMDKKLDPIEAVELSWKLTKGYGWRIFAMGLTSVFIMIFGLILFIVG
                     IFPAIMWISSSFASLYQSVLLASEKPAEIIAA"
     gene            complement(3089..5371)
                     /locus_tag="PK288_02245"
     CDS             complement(3089..5371)
                     /locus_tag="PK288_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPM17766.1"
                     /translation="MTQNYLKELNEAQRQAVVNTEGPALVIAGAGAGKTRVLTYRIVH
                     LLQKGIPAHRILALTFTNKAAREMKDRITRIAGPDITRHLWMGTFHSIFARILRTEGP
                     RLGYKPNFTIYDSSDSKSLVRTIIRELNLDDNIYKAGMISARISTAKNNLITASMYSA
                     NSSLQEHDKMNRMPMLADIYRNYAARCFKSNAMDFDDLLLNTNILLRDFPEVLALYQS
                     RFQYILVDEYQDTNYSQYLIIRKLAEGHKNICVVGDDAQSIYSFRGARIENILEFKND
                     YPDYKLYKLEQNYRSTQTIVNAANSIIANNEGQIRKIVFSENERGEKLQVLQALTDSE
                     EGFNIASDIFDKRFSMRLLWSDFAILYRTNAQSRIFEETLRKKNIPYKIYGGLSFYER
                     KEIKDILAYFRMVINPDDEEALKRTINYPKRGIGDTTVKKIFELAEQFRVTPWQIISG
                     ESGYIQQFNQGAVNKLTAYAELIGNFRSFNDDKDAYTLAREIALATGITRELREGRSP
                     EEVSRYENFEELLNGIKVFSESAEANGEASDLEAYISSVSLLTDQDNEKEEDRDKVTL
                     MTMHSAKGLEFKLVYIAGMEDTLFPSPMSAGNPRELEEERRLFYVAVTRAQKLATLSY
                     ALNRYKWGNLERCAPSRFIREIDRKFLEYPQSGGKTVRARNHGLMENQDTAIEAPRHD
                     SPPRGFRKVSYPAAGREFSRQSFSATEFAEGDIVTHERFGRGEIINIEGEPPNTTATV
                     EFENNGRKKLLLRFARLSKL"
     gene            complement(5670..6020)
                     /locus_tag="PK288_02250"
     CDS             complement(5670..6020)
                     /locus_tag="PK288_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17767.1"
                     /translation="MKKLMFILVLAVLAMTVDAQRTAVKTADLQKSITDWITKDYAGF
                     AITKADKVVANNVTTFEVVITKGSNSETLVFDKDGKFMHKMALKSGSPEKHTAGSSPM
                     AHHKPETKPKTGKK"
     gene            complement(6129..7349)
                     /locus_tag="PK288_02255"
     CDS             complement(6129..7349)
                     /locus_tag="PK288_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM17768.1"
                     /translation="MLGGVLLYFSTRKMVYDQIDNSLLTELEIIRDQIEQTKSIPDFS
                     ARPGHQLEVRLISGKVRPVQVINDTIITDTTGYEHPYRYILYRGNLDENTGFSVVISQ
                     TLREKIVLLEYIGVYIFFLFLSLFFISIFLNYLFSRRLWRPFYITVDQAEKFDIQSDR
                     LPVLPQTNISEFERLNAVIGQMTKKMRDDYLNLKEYHENAAHEIQTPLAVIRSKLEIL
                     AQRKELRKESLNLILSINEAATRLYKINQGLLMISKIDNMYFQDETDISLAETIEKIL
                     LGYREIMNLRKIKVDFLAGDKAMVRMNEMLADTLVSNLISNAVRYNFDGGFIRCQVHK
                     DKLIISNSGPPLKTDTAILFRRFQKAGDNPQSVGLGLSIVKKIVEAYSMKIVYTCVGK
                     VHEITLLFASPAGI"
     gene            complement(7403..8080)
                     /locus_tag="PK288_02260"
     CDS             complement(7403..8080)
                     /locus_tag="PK288_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM17769.1"
                     /translation="MKILIVEDDNSLSAAINDYLRAEGHICELARDFKQALFKVADNR
                     YDCIILDIGLPDRSGLDIIKELKENKVSDGILILSARDSLEDKLTGLRTGADDYLTKP
                     FHLAELSARINSIYRRNNLSGQNEVVFEELRIRTSDNQAFVNNRQLNLTKKEYDLLLF
                     FIINRNRVITKESIVEHLWGDDVILTDSFDFVYTHVKNLRKKIKTEGGRNYIRCIYGF
                     GYKFGDP"
     gene            complement(8238..8789)
                     /locus_tag="PK288_02265"
     CDS             complement(8238..8789)
                     /locus_tag="PK288_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17770.1"
                     /translation="MKKLILSLIGFTAFIFPLLAQVDYDQGENDEIPVENLILKKEQI
                     PAAIVQAVTETFKSGKAFKWGKFPYVLEKYGWIVSKSAGDQKPDRYEAYIKADDGSEI
                     YAIYNPDGTIVQSRIIRKNAELPHLVAEKLAKSKYAGWKIVGDREVIKYYNSPQDIEE
                     HFRITVEKNGLKKTVSFRLNDKA"
     gene            9000..11435
                     /locus_tag="PK288_02270"
     CDS             9000..11435
                     /locus_tag="PK288_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HPM17771.1"
                     /translation="MTKFLPLLFFLIVLDSCSGRNMQRRELRIWFDHPADALVPDVED
                     GWRNDAEWLKALPVGNGFIGAMVFGDVNTERIQLNNKNLWSGSPDDNDNPEASEHLYR
                     IRRLLFEGRYREATELTLRTQVCRGAGSGHANGAGLPFGCYQTLGDLWLDFNRDQGYE
                     KYHRELNLDHGVVSVSYTQDGIKYKREVFASYPDRALVIHISSDTRGSVTFDSWFTRP
                     ERSRTSSSDGHILMSGTLSDGKGGEGMSYAVRVKAVAAGGTVTCSDGRISVNKADEAL
                     LVLVSSTDYKQEYPDYLDGDPVTISLEQLNEASFRPFGELLKRHIADYRSLFGRVSLD
                     LAGREKDTVPTDRRLLNQLEDPDDMILHETYFQFGRYLLISSSREGSLPANLQGIWAN
                     KIQTPWNCDYHTNINVQMNYWPADVGNLSECYSPLSDLILSLVKPGERTAAVQYGAGG
                     WCVHPITNVWGFTAPGEHPGWGLHVVAGGWLCQHLWDHFTFTLDTAYLRKVYPVMLGS
                     ARFYLDWLVEDPVSGELVSGPAGSPENSFIAPDGSTAQISMGPSHDQEVIYDLFSNVL
                     NASAVLNDSDPLLAVIKAARSRLAMPQIGSDGRLMEWREEFREAEPFHRHVSHLYMLH
                     PGNLIDPVTTPQLADAARKSLEARTDIGTGWSLAWKINFWARLHNGNRAYKLLKNLLR
                     PITTYSVNMSDAGGTYQNLFCGHPPFQIDGNFGATAGIAEMLLQSHTKEGERYVIQVL
                     PALPDAWKDGEVRGLKARGGFEADIKWKTGKLVSCTIRSNAGAGLKICYGPRSVVIDS
                     KPGMTYHFNGNLE"
     gene            11688..12911
                     /locus_tag="PK288_02275"
     CDS             11688..12911
                     /locus_tag="PK288_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM17772.1"
                     /translation="MRYYLFTVAILLTVTLEAQLTAPGSNAVRYTSYPSAPSLRHPIF
                     IFCNASGNQKGELNAVSPGGTGPFTFSWSRWNDATRNFTIPVKNETGVFSSSVTNLDE
                     GGYRVNITGASGYSASLTGWIFLDSPHSKAELRHFTCDYVALGGEAAVDTFYYRDINS
                     GSRIILPNRVRFLWSSQPASSIPYPDIELNPQTFNPPLDDVTYNLHVTDSFSCTTESS
                     FFYESIHVKADFTVDPAKGEAPLEVTFTDKSVRALKYKWIFGDDTFSNDISPAPHTYF
                     IPGDYKVRLIVESSLHCIDSMTFDKIVVDKSGLSIPNVFTPNDDGMNDMFMVDKTSLR
                     YISVDIYSRSGIKVYEFYGEGEILKNWEGWNGNIRGTSLKASPGVYYYIIKARGWDNV
                     RYDNKEYRGFVYLYR"
     gene            complement(12947..14074)
                     /locus_tag="PK288_02280"
     CDS             complement(12947..14074)
                     /locus_tag="PK288_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HPM17773.1"
                     /translation="MQSPGSPFTGWYVAGNAGELETPALILYPERIEANIRKMIDVAG
                     SADRLRPHVKTHKIPEIVQLQVRHGIRKVKCSTIAETEMAARAGADDILLAYQPVGPN
                     IQRFFSLPRKFPSKKFSCISDCESILRELSDHASVTGYKAHIWLDINAGMNRTGIEPG
                     EKARELFRMISLLPGLVPAGLHVYDGHINERNVENRIKICDEAFAPVRSLIEDISIMT
                     GAPVKVIAGGTPTFPVHARRENTELSPGTTLLWDYGYGSSFSDMQFLYAAVLLTRVIS
                     KPSGNKICIDLGHKAVASEMPHPRIHIEGMHDYRVTGHSEEHMVIETTEAGKINVGDH
                     LYAIPWHICPTVDRYDTVYVSEGGMVTGQWQVEARKRKINL"
     gene            complement(14076..14543)
                     /locus_tag="PK288_02285"
     CDS             complement(14076..14543)
                     /locus_tag="PK288_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPM17774.1"
                     /translation="MEINPESRLAELGLILPPAPDPAGVYKPVLVVENLLYVSGQAPL
                     RSDGTLIKGRLGADLGLEDGKLAARQVGLAMLATIRKHFGDLSRIRRIVKVLGMVNST
                     QDFDQHPAVINGFSELMADVFGSENGIGVRSAVGMILPMNIAVEIEAVFEIHR"
     gene            complement(14632..15948)
                     /locus_tag="PK288_02290"
     CDS             complement(14632..15948)
                     /locus_tag="PK288_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HPM17775.1"
                     /translation="MSIFWVVIGILFLVLLIIVLKYNPFIAFVITSLFVGVAEGMKAI
                     DVITSIQQGIGDTLGSLVLILGFGAMLGKMVADSGAAQRISTKLTDLFGIGNIQWAMV
                     LTGFMVGIPMFYNVGFVVLIPLVFTVAASTGLPLLYVAIPLLASLSVTHGFLPPHPSP
                     IAICTMFGADIGKTIGFGIIISIPAIIVAGPFFGKTLKKIKASPIKEFVSSRAMSDDE
                     MPGIGISVLTTLMPVILIGAYSVSTIFLTEGNRLRSVLEFIGDPVMAMLISLILSVYL
                     LGTRRGKSMTDIMNSLGNSIGSITMVLLIIAGAGAFKQVLVSSGVSDDIAGLLTKSSM
                     SPLLLAWLIAAVIRICIGSATVAGLTTAGILLPLATGGTVNKELLVLSIGAGSLMLSH
                     INDSGFWMYKEYFNLSVKDTFKTWTVMETIVSVIGLLGVLVADLLI"
     gene            16206..16883
                     /locus_tag="PK288_02295"
     CDS             16206..16883
                     /locus_tag="PK288_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPM17776.1"
                     /translation="MKTKIVYSFLHDLFSGMDYRQRITEEAARLFRQYGIKAVTMDML
                     ANNLGISKRTIYEVFKDKNELLAGVVSHMVIRQKELINRIFNESENVIEAIFRLLETM
                     SEHIRRISPAFRLDMMKFSNDAIMDLKKTGHFPYPYSTPYLIENGIKEGVFRDDIDVE
                     ITNKCLFELIRMSDRGDDPEQDANHRMHVIRDVYINYLRGISTPKGLELINYYETHMI
                     DQKPRHE"
     gene            17224..18561
                     /locus_tag="PK288_02300"
     CDS             17224..18561
                     /locus_tag="PK288_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM17777.1"
                     /translation="MKRRLVIILILSGFLHGYSQQAREGGSELKLSLRDAQDYAILNN
                     KSVLAARADVMASKAAVWETISAALPQLNANGTFADNLKLMTTLLPGEFFGQPGTKIP
                     VTFGSKYNSSASFQANLLIFNAPLFLGIETSKLAYKLNANNLELSELDTKEAVSSAYF
                     LILVSEKSLEILNANIANLNETLRSTRSMYSAGMAESTDVDQMVSNVTMMDNNRSSLL
                     RTIELNYNLLRFQLGVSADTRIILTDNLEGLTNAVNVEALMSQDFDHTKNINYKLVEG
                     QELMSELALKSQKASVLPTLAGFYNYGTSGMGDKMRDLSWFPNSMAGLQVSVPIFASG
                     ERYAKIQKAKYNLNKARITRNMIEDQLLIQEKQLRYNLVNANLQYQSQKENVDVLERV
                     YKSTENKYMQGMASSLELTQANTQYLQAENNYVSALMNLLLTKLALDKLLNNL"
     gene            18592..19656
                     /locus_tag="PK288_02305"
     CDS             18592..19656
                     /locus_tag="PK288_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM17778.1"
                     /translation="MKYIKTIPVLFLSCMLVAACSSRKKSDNSGALAATASPDRTAVP
                     VKILSLAKSTISRTIDYTATILPYEEVNVVPSTPGRIEKINVEVGDRVSKGDELFLMD
                     RTQLYQQKIQLGSLAKDLTRLDTLLRTGSIKQQQYDQLKTQYEVTRSAVEFMEANTHL
                     KAPFSGVVTGKYFEDGEMYSGTPTTQSGRSAVVTLMQVNPLKIDVNISEQYYPLIKKG
                     MKASVVADVYENEVFTGSVFRKAPTINAATRSFVTEIELPNRNDLLKPGMFVRVSMDL
                     GEVEAFVVPSNAVLMQEGTNIRYIFLEENGRAKRVEVTLGKRFDDKLEVLSEFLKEGT
                     GLVTEGQAKLIDGDRIEVIK"
     gene            19682..22840
                     /locus_tag="PK288_02310"
     CDS             19682..22840
                     /locus_tag="PK288_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM17779.1"
                     /translation="MSIYSSSVKRPVTTILIFVGLMVMGLYSLVKLPVDLYPEMELPY
                     VVVYTTYPGASASDIESNVTRPLENSLNSVSKLKEITSRSSDAVSAIFMEFEYGTDLD
                     EASNDIRSAISFTERLLPDGSEKPTIVKFNTTMMPIMFYAITARESYAGLEKILDEKV
                     VNPLNRIEGVGAVSFSGVPGRKIYIDVDPRKMEAYNLTIEQIGNILRAENMNLPVGYI
                     EMGNTDYPLRIQGEFPESDVVKNIVVSSYGGSNIYLKDIAEVRDTIRESKLDTKINGV
                     KGMGLLVQKQSGGNTVQVTRKIEKSLVRLEKSLPPDVKITKLFDSASFIRDSISNLTE
                     TLMYAAIFVILVVLFFLGRWRATFIIILTIPISLVVAFIYLFVSDASINIISLTSLSI
                     AIGMVVDDAIVVLENITKHIERGSRPREAAIYATNEVWLAVIVTTATVVAVFFPLTFV
                     SGLTGVLFKQLGMSVTITIVTSVFAAITLTPTLSALLLKWYPIKEDAPFWTYDGSIRK
                     SLDRFDRFYEKTLKWALRHKTFVVIVSLLVFIGSMSLFFFIKTEFFPQADESRITAKV
                     ELQTGTRVDQTIRTADRLDSLIRAKYPEVRLISTSAGADDQGGFAAMFSNQGTHTITY
                     SIRLVPVKERKRSSMEIADELRTDFAKYPEIIHYTVSTSDNMGSFGGSTVDVEVYGYD
                     IAQTNIVAEDLAARIKNIRGAKDVTVSRDKSKPELQIVFDQNKMMANGLNTAMASAAI
                     RNRIDGLVATRLRQYGEEYDVVVRFKRSSTSTLTDIENIGITNATGQLIRLAEIARIK
                     EYWAPPSIEHKRKERIVTVSFTPFKRSLSELKVEVEKAINSINMPAGVMVQISGSIEE
                     QRESFMDIGFLLVISLILVYLIMASQFESLKMPFIIMFSIPFAFSGVAIALFVTNTAL
                     SVISAIGAVMLIGIVVKNAIVLVDFINLMRERGNELYEAIAISGRSRLRPVFMTSLTT
                     IFGMFPLAVSSGTGSELWSPMGISVIGGLVFSTIVTLVLVPVVYAIFAKHGERNKNLA
                     IYMEMNFMNDNGNEAGDTFTDK"
     gene            22878..23171
                     /locus_tag="PK288_02315"
     CDS             22878..23171
                     /locus_tag="PK288_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPM17780.1"
                     /translation="MKAVMIIYNQAHSEKIEFLLDKVGVKGYTLWENVKGRGSDTGVP
                     HFGTHTWPEINKSVLAIVDDPMVDEILDKVKKIDAINYDVGIRAFVWDILKTA"
     gene            23540..24922
                     /locus_tag="PK288_02320"
     CDS             23540..24922
                     /locus_tag="PK288_02320"
                     /inference="COORDINATES: protein motif:HMM:NF017817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM17781.1"
                     /translation="MKYDVVIVGGGVSGTALLYTLARYTDIRNIAIIEKCCDFGLINS
                     SSRMNSQTLHFGDIETNYSLEKAKRVKRSADMVMNFLETEKAVNGDERLFVKVPKMIL
                     AVGKEETGKLRARYEEIRELFPRLRMIGKEDIARLEPRIVVGRDPDQDLLALFTRDGY
                     TVDFGKLSHAFVDKARQENPDISINLSTRVLKVVRNGNEYDILTDKGSFKSETVVVAA
                     GAHSLLLAKSLGYGKNYTILSMAGSFYFGPAVLGGKVYTMQEPKLPFAAIHGDPEVHD
                     RNTTRFGPTAKPILLLERYNASTFTGYLKTCGLNIRSLISLVKIASDKIVFRFLLKNL
                     IYDIPAIGKKAFLKEIRKIVPGISGDEVKFARKIGGTRPQVVNINTGKLEMGEAKIRG
                     EKIIFNVTPSPGASTCLGNAFEDTLLLMGFLDNKYKFERELFEKELINRNEDVTDQIQ
                     RENLINSPVV"
     gene            24977..27256
                     /locus_tag="PK288_02325"
     CDS             24977..27256
                     /locus_tag="PK288_02325"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPM17782.1"
                     /translation="MDNSYYKDLKLFYRNLPDKISTIRNNLQQPLTLSEKILYSHLFE
                     SKSLVIQKRGVDYVEFAPDRVAMQDATAQMALLQFMMAGRNKSAVPASVHCDHLIVAR
                     SGDVEDLKNARITNREVYDFLSSACLKYEIDFWEPGAGIIHQTILENYAFPGGMMIGT
                     DSHTVNAGGMGMIAIGVGGADAVDVMSGMGWELKFPRIIGVRLSGRLNGWTSAKDVIL
                     KLAGILTVKGGTGCIIEYFGEGSGSLSCTGKATICNMGAEVGATCSIFGFDEKIASYL
                     AATGRKEMADMAYALKENLIADPEVCSDPEKYYDQVIDIDLSELEPYINGPFTPDLAT
                     PVSKMKEAARNAGWPVKVDVGLIGSCTNSSYEDISRAASIARDALDKKLSLKSEFTIT
                     PGSEQIRAITERDGYFDILRKIGGRIFANACGPCIGQWDREGAGKSGVNTIVHSFNRN
                     FARRNDGNPNTHAFVASPEIVTALAIAGELTFNPLTDYLVNDEGVKVRLKEPEGFEIP
                     ISGFGESGRNLHVQPAERESVEIKIDPQSDRLQYLQPFEKWDGSDVMELALLIRVKGK
                     CTTDHISMAGFWLKYRGHLENISENYMIGALNSYNDKTNSVLNQLTGKYETVPKAARH
                     YRDMNMGTIVVGEENFGEGSSREHAAMEPRFLGVKAILVKSFARIHETNLKKQGLLAL
                     TFNNKDDYDKIQEWDKINILGLKKMSPGRPIELILRHRDGSTEKILANHSYNEAQIEW
                     FRAGSALNLIRIRNQDNSI"
     gene            27282..28544
                     /gene="icd"
                     /locus_tag="PK288_02330"
     CDS             27282..28544
                     /gene="icd"
                     /locus_tag="PK288_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPM17783.1"
                     /translation="MTGEIRKEKNNLIVPDFPVIPFIEGDGTGPDIWKASVRVFNAAV
                     EKVYGSEKRIVWKEVLAGEKAFRETGNWLPEETLSTFRHYLVGIKGPLTTPVGEGMRS
                     LNVALRQELDLYTCYRPVRYFDGVPSPVRHPEKVNMYIFRENTEDIYAGIEYLNGTPE
                     AEKIRKFLSDEMGVRKIRFPESSSIGIKPVSVEGTERLVRQAIKFAVDRNLPSVTLVH
                     KGNIMKFTEGAFMKWGYALAEREFSNEVFTWNRYREIADREGAAPADAALAEAVSNRK
                     VIIKDVIADAFLQQILTRPEDYSVIATLNLNGDYISDALAAIVGGIGIAPGANINFES
                     GHAIFEATHGTAPKYADQDKVNPGSVILSGALMFEFIGWKDVADRIYAGLEKTIRSGL
                     VTYDFHRLMNNANLLKTSEFASAIINNL"
     gene            28573..29940
                     /locus_tag="PK288_02335"
     CDS             28573..29940
                     /locus_tag="PK288_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPM17784.1"
                     /translation="MTNDKFFPRLEDSVRESCQIENSLFEKFDVKRGLRNTDGTGVLV
                     GLTNICDVVGYKKEDGKVIAIPGKLLYRGIEIEDITAGFQKEGRHGFDETVYLLLTGN
                     LPTSEELKDFSAHLASLRSLPDDFVKDMILSMKGRDVLNVLARSVLGLYTLDQRPDDI
                     SLPNMIRQSLNLIAKFPTIVAYSYQALRHLYKGKTLSIRHPQPKLTVAENFLYLIKGE
                     KKGYSKLESDLLDLLLVLHAEHGGGNNSTFTMRVTSSSETDIYSSVTAAIGSLKGPLH
                     GGANVRVLEMMENIKSHVKDWKNEAEVEAYLLKILNKKANDHSGKIYGIGHAVYTISD
                     PRAGLLKAKARELAIEKGRLDEFELYEMVERITPEVFRVFKGEKTAKVVCINVDFYSG
                     FVYSCLEISKELYTPLFAIARVAGWCAHRIEELTFSAKRLIRPAFKYVYGQHEYIPMS
                     DRRKF"
     gene            29960..30625
                     /locus_tag="PK288_02340"
     CDS             29960..30625
                     /locus_tag="PK288_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM17785.1"
                     /translation="MNTLPDDRFQLAFDKILEGNLQDAAGILEEEHVKNPGNIAVLLE
                     LGNIYYILCEMSKSIEYYKKILEIKPLSPYVMYRVGVALYQSTHFSEAAEVFNRIIES
                     EKHLPMTYMWLGLCYYHLGQEEKSIASYRKLIEYGPETVMANYYMGVAMKSSGKYHDA
                     IIHFEKLLHQTDQHVSALYHLGRTYMKDYQFEKAKVYFKMAIDLDPENKNASEMYDYL
                     ING"
     gene            complement(30633..32789)
                     /locus_tag="PK288_02345"
     CDS             complement(30633..32789)
                     /locus_tag="PK288_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPM17786.1"
                     /translation="MKTYQTGQIKSIAILGNSGSGKTTLCEAMLFNGGVIERRGTVDA
                     RNTVSDYRPVEHENGNSIYSTVLYSEFRNKKINIIDTPGLDDFSGNVVSSLFATDCAV
                     LTINVQNGVEVGSEIAFRHAEKAHKPLILVVNGLDHEKANFERSIEMMKDRFGSNTNV
                     VQYPVNEGAGFNSIIDVLRMKMLQYPVEGGISKILDIPEDQMEKALELHNALVEKAAE
                     SDESLMEQFFANDSLTEEEIRRGIAKGIVTRGIFPVFCLSAKNNIGVDSLMEFIVSEA
                     PSITDMPPPVNSRGDLVKADPAGPASVYVFKSSIEEHIGEINYFRVFSGKITENMEVV
                     NSNNGVKERLSQLYVCAGKNRVRVQELNTGDIGAFVKLKNTKADHTLNAPGNDWKYAG
                     ISYPEPKYRTAIKAQSESDDEKLGEALGKIHLEDPTIIVEYSKELKQILVHGQGEYHL
                     NIMKWHLDNIYKIPTNFNPPRIPYRETITKAAQAEYRHKKQSGGAGQFGEVHLIIEPY
                     TEGSPELTMMKFGNKEIKLSVRAKEEIDLDWGGRLIYVNCIVGGSIDARFMPAILKGI
                     MEKMEVGPLTGSYARDIRVYVYDGKMHPVDSNEISFRLAGRNAFSMAFKNAGPKILEP
                     IYDVEIMVPADRMGDVISDLQGRRGMVLGMSSEKRFEVVRARVPLAEMNKYSTALSSI
                     TGGRAMYTMKFSEYAQVPGDVQDAVIKAYHEEEEDE"
     gene            33205..33579
                     /gene="rpsL"
                     /locus_tag="PK288_02350"
     CDS             33205..33579
                     /gene="rpsL"
                     /locus_tag="PK288_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPM17787.1"
                     /translation="MPTIQQLVRKGRKKLIDKSKAPALDSCPQRRGVCVRVYTTTPKK
                     PNSAMRKVARVKLTNQKEVNAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHLVRGAL
                     DTSGVDGRTQRRSKYGAKRPKK"
     gene            33599..34075
                     /gene="rpsG"
                     /locus_tag="PK288_02355"
     CDS             33599..34075
                     /gene="rpsG"
                     /locus_tag="PK288_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPM17788.1"
                     /translation="MRKTKPKKRILLPDPKYNDPYVTRFVNNLMYSGKKNLAFKIFYD
                     SLEIVGDKFRNEGKTPLEIFKQALENVTPQVEVKARRVGGATFQVPMEIRADRKISIS
                     MKNMISFARKRTGHSMAERLAAEVMAAYKLEGGAYKKKEDTHRMAEANKAFAHFRF"
     gene            34109..36211
                     /gene="fusA"
                     /locus_tag="PK288_02360"
     CDS             34109..36211
                     /gene="fusA"
                     /locus_tag="PK288_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPM17789.1"
                     /translation="MDSNLKYTRNIGIMAHIDAGKTTTTERILFYTERTHRMGEVHDG
                     NAQMDWMVQEQERGITITSAATTTFWKYRGDNYKINIIDTPGHVDFTVEVERSLRVLD
                     GAVAIFCAVGGVEPQSETVWRQANKYKVPRIAFINKMDRAGADFFGVVQQIREKLGAN
                     PVPIQLPIGSEDDFKGVVDLVSMKTVVYNDNDATGSHFTVGEIPDELIDEANEWRGRL
                     IESVADVDDTLLARYLEDQYSITSDEILDALRKSSVHGLAVPVLCGASFRNKGVQRLL
                     DAIAAFLPSPLDIGPVTGIDPRNDSEVSREPDVNEPLAALAFKIATDPFVGRLVFLRV
                     YSGVLKAGDTVLNTRTGKRERINRLFQMHANKQNPKEVIYAGDICAGVGFKDLKTGDT
                     LCAINKPILLESMDFPDPVIGIAVEPKTQADVDKLSLALNKLAEEDPTFQVRIDPDSG
                     QTIINGMGELHLEILIDRLKREFKIECNQGVPQVAYKEAITTSTEFREIFRKQTGGKG
                     KFADIAVLMMPGENGVRGLQFVNEVRGGNIPKEFIPSVEKGFREAMANGPLAGFPLES
                     LKIVLKDGSYHPVDSDSLSFEIAAKQAFRKYAGKCNPVILEPIMSTEVVTPEEYVGDV
                     IGDFNRRRGKVEGMESKAGSRVIRAKVPLAEKFGYVTVLRTLTSGRATSTMEFSHYEE
                     VPAEIANRIVEITKGKIL"
     gene            36214..36519
                     /gene="rpsJ"
                     /locus_tag="PK288_02365"
     CDS             36214..36519
                     /gene="rpsJ"
                     /locus_tag="PK288_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPM17790.1"
                     /translation="MSQKIRIKLKSYDHNLVDKSAEKIVKTVKSTGAVVSGPIPLPTH
                     KRIYTVLRSPFVNKKAREQFQLSSYKRLLDIYSSTPKTIDALMKLELPSGVEVEIKV"
     gene            36541..37161
                     /gene="rplC"
                     /locus_tag="PK288_02370"
     CDS             36541..37161
                     /gene="rplC"
                     /locus_tag="PK288_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPM17791.1"
                     /translation="MQGLIGKKVGMTSVFDEDGKNIPCTVLQAGPCVVTQVKTREKDG
                     YSAVQLGFDEKKEKHTTKAELGHFRKAGTTPRKKLVEFTGFDEGQVKEGEVLTAEFFT
                     HTDWVDVRGWSKGKGFQGVVKRHGFGGVGDQTHGQHNRGRAPGSLGASSFPSRVLPGM
                     KMAGRTGGNKVISYSMRVVKLMTDKNLIIVRGSVPGPKGGYVIITK"
     gene            37161..37820
                     /gene="rplD"
                     /locus_tag="PK288_02375"
     CDS             37161..37820
                     /gene="rplD"
                     /locus_tag="PK288_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPM17792.1"
                     /translation="MELAILNIDGKETGRTVNLNDSVFGIRPNDHAIYLDTKQFLANQ
                     RQGTHKSKQRGEIAGSTRKIKRQKGTGTARAGSVKNPLFRGGGRIFGPEPRFYGFKLN
                     KKVKQLARKSALSYKAATNNIIVVEDFSFETPKTKEILKVGDSLNIMRKKSLIVLPEQ
                     NKNIYLSSRNVQDIEVVTAEELNTYIIMRASTLVLLESAVDVLQAAFENQKTLSDEYF
                     A"
     gene            37804..38094
                     /gene="rplW"
                     /locus_tag="PK288_02380"
     CDS             37804..38094
                     /gene="rplW"
                     /locus_tag="PK288_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPM17793.1"
                     /translation="MNILLKPIVSEKMTNQGDKYNRYGFYVAKNANKLQIKKAIEELY
                     GVTVDSVNTMRYGGKRKSRNTRSGYLVGKTAATKKAVVTLAEGNKIDFYSNI"
     gene            38103..38927
                     /gene="rplB"
                     /locus_tag="PK288_02385"
     CDS             38103..38927
                     /gene="rplB"
                     /locus_tag="PK288_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPM17794.1"
                     /translation="MAVRKIKPVTPGQRNKIISAFDKITSAVPEKSLLRPVRKTGGRN
                     ASGRQTMRYIGGGHKRMYRIIDFLRQKDDMHATVRSIEYDPNRSSRIALVVYEDGEKR
                     YIVAPAGLEVGQKIISGKNASPEIGNTMFLNDIPLGTVIHNIELKPGKGGALARSAGT
                     YAQLSAREGKYVTIKLPSGETRIVLTSCRATIGAVSNPDHSLEKSGKAGRTRWQGRRP
                     RVRGVVMNPVDHPMGGGEGRASGGHPRSRKGVPAKGYKTRDKKKYSDKYIIERRKK"
BASE COUNT        11126 a         8318 c         9019 g        10485 t
ORIGIN      
        1 tgcccgaaat cagaaggatt gacagaaaga ctggagcatg gtgcctgacg agtgcagtcg
       61 ccctgtgggt attgctttgg ttcctgctcg acaaggcaac cggctcaccg gtagcactgt
      121 gggacggact catagcctcc ctgtccgttg ttgctacttg gatgcttacc agaaaatact
      181 tcgagctttg gtttgtttgg ctggctgcca acgcaatcgc agtggttgta tatctgtctt
      241 cgcaattgta ccctactgga ctgcttttcc ttgtatattt ctcaatgtcc gtagtggggg
      301 caaaggaatg gcgcagagta atgaccgcag gaagtaaata ttgaattatt caggcaaaaa
      361 ttgtacttaa atgaacatag tgatccttgc aaacggcaat tatccgtcgc atgaagtgcc
      421 gcttggatac ctgggaagag ccgacctgat tgtctgctgc gacggagccg tggaaaaact
      481 tgtgaccaac gggctggagc cggggataat catcggtgac ctcgactcgg taacgcccgc
      541 cctgaaaaga aaatatgccg gaataattgt gcaggatctc gatcaggata ccaacgacct
      601 gacaaaggca gtcaactggt gtgttgacag gaattatgac aatcttgtaa tattgggagc
      661 taccggaaat cgtgaagatc atactatcgg caatatctca cttctggctg agtatgtaaa
      721 aaagaccaca gtaaagatga tcaccgatac cggtatcata acaccgcatt tgacgagcag
      781 cagattctca tcattccgcg gacagcagat ctcaattttt tcagttgatc cggagaccgg
      841 aattacttcc gtgggcctga aatataagct tgaaaatatg aaattgcgca actggtggca
      901 ggcaacattg aatgaagcag aaggaaatac cttcgatctc atatttgaag gaggccctct
      961 gatagttttt cagaagttca ggaaataacg acatagatca aagacaaaca tgttcggaat
     1021 tatcatttta ccatgtcatt aaccaggttg atgagctgaa ccctgctttt cacatttgtt
     1081 tttatatata tcctgtgggt gtgatccttt accgtttgca gactgatgaa taatttatcg
     1141 gagatttccc tgttactcag tccgctgcat atctctctca ccacttccga ctcccttggt
     1201 gaaatgtcaa accgcttgca gaactgctca agggtaagga aattattcgt cctgtcaata
     1261 ataaccggag aaatgataag ataactgaaa taaacgggaa gaaagatatt tccgaggaag
     1321 aatgtgaaaa tgaaaataat tgcaacccaa ggacttaaat cgattaatac cagtgggatg
     1381 cattgaatga tcattattaa aaatatagcc ggggcaataa cttttctgtc atgttctttg
     1441 atcagtgatt ttcctttcac gggaaggtga ataaggtatg ccgcaatgaa tgtatgcacc
     1501 aggttcataa gaatataata attccttatg attagcacag tattccttga attcagcctt
     1561 gtcatgaaaa agtaccccag aacagccagt ataatgaaat tggacaaaag aaagccggca
     1621 ataaaccagt tattgctttt ccttcctgac agaattcctg aaaaccttat caacattagc
     1681 catgcaaata tcaggaatgg taatccaagc agcgttgtta tattgtttac cctgataaga
     1741 atatcgggag ggatgaggga tgacagaaac atccttgcga caacctggcc ccatattccg
     1801 taaaatccaa aggcatatat gaaaacctga tagtacagga gggtggaata tatcccggca
     1861 ccgttcctgc tcctcagcct ggaggaaagt atgatccctc ctgccgaaag tgccactgaa
     1921 aggataaaaa taagatatcc caggtattca aacatatgat aaagataaac aaagagtatt
     1981 gattaattac ggaaaaagca agatatagag cctcgccggg gaaaaacgaa ctatccggca
     2041 agatggcgta tcattgctgg caatgctgta aaatgggcgt atttagtgca ttatactact
     2101 taagtattgg tccaatacct aagtagtgaa attgaaaagt cttttctgat cactaccaag
     2161 gtattgacac cgctaaaaaa ggagattgta atttcgatcc gtattccggt tcattatacg
     2221 gcaatttccg gaaatctgat tatacaatta aaataaacac taaaatggaa acagaaaaaa
     2281 attacaatct gatcccaact tacgggaaca gcttcaggac aggatggaaa gtgatgtttg
     2341 acaattttct aaggctgctg cttgttgttt ttgtactggc tttgctgacg gctcctctga
     2401 agatgtttga gttgaaactg gaaccgtccg atttccacgg acagcttgat aaatggagcg
     2461 atatatggga gtatgatatg ggaaaaatcg ttacgctggg aacgctgggc atggttgcgg
     2521 cctttgcagg gcttctggca ctggcataca cttttctggc aattccggtg gtttcgtatg
     2581 gtggaaacat gatttttgtc caggcggtac gaagaataaa acccgaattt gaccttctta
     2641 taaggggatt ccgggagaat tatctgcata tcgtcctttc caatctgctg ttgtttgcac
     2701 tgattgttct gggctgcttt gccctgatca ttccgggtat tatcattgcc tgcaggctgg
     2761 tgtttgtacc atatattgtt atggataaaa agctcgatcc catagaagct gttgagctga
     2821 gctggaaact gacaaaaggg tatggctgga ggatctttgc aatgggactg acatcagtat
     2881 ttattatgat tttcggactt atcctgttta ttgtgggtat tttcccggca ataatgtgga
     2941 tttcatcatc gtttgcctca ctctaccagt cggttctgct tgccagtgaa aaaccggctg
     3001 aaattattgc agcgtaaagc aatccgggag aaggagaact ccggatcctt ttcccatctt
     3061 caacggtctg gctgattcgg gattgttttc ataatttact gagccttgca aaccggagaa
     3121 gcagtttttt tctaccgtta ttttcaaatt ctactgtggc tgttgtattc ggaggttcgc
     3181 cttcaatatt gattatttca ccccggccga atctttcatg cgtaacaata tctccttcgg
     3241 caaattccgt ggctgaaaag gattgacggc tgaattccct gccggcggcg ggatacgata
     3301 ccttcctgaa cccgcgaggt ggtgaatcgt gccggggggc ctcaatcgca gtgtcctgat
     3361 tttccatcag gccatggttc ctggctctga ccgttttgcc gcctgattgc gggtattcaa
     3421 ggaatttcct gtctatttcg cgaataaacc tgctgggtgc gcatctttca aggtttcccc
     3481 atttataccg gttcagtgca tagcttaagg tggccagttt ctgtgccctt gtcacagcaa
     3541 cgtaaaacag gcggcgctct tcttccagtt ccctaggatt tccggccgac atgggtgaag
     3601 gaaaaagagt gtcttccatc ccggcaatgt agacaagctt gaattcaaga ccttttgctg
     3661 aatgcatggt catcagggtc accttgtcgc gatcctcttc cttttcatta tcctggtcgg
     3721 ttaagagaga aacgctggaa atgtatgcct ccagatcaga agcttcccca ttggcttccg
     3781 ccgattctga aaacaccttg ataccgttga gcagttcctc aaaattttca tagcggctta
     3841 cttcctcagg cgatctgccc tcccttagtt ccctggtgat acctgtggca agcgctattt
     3901 cgcgggccag ggtgtaagca tccttatcat cattgaaact cctgaaattc cctatcagtt
     3961 cagcatatgc tgttaattta ttaacagcac cctgattgaa ttgttgtata taccctgatt
     4021 ctccgcttat tatctgccag ggagttactc tgaattgttc cgcaagctcg aaaatcttct
     4081 taacagtagt gtcgccgata cctcttttgg gataattaat ggttcttttc agagcctctt
     4141 catcatcggg attgatcacc atcctgaaat aagccaggat gtcttttatc tcctttcttt
     4201 catagaaaga cagccctcca tagattttgt atggtatgtt ctttttcctc agggtctctt
     4261 caaatatcct cgattgtgca tttgtccggt acaaaatggc aaagtccgac caaagcagcc
     4321 tcatggaaaa tcttttgtca aaaatatccg aggcaatgtt aaatccttct tcagaatctg
     4381 taagggcctg gagtacctgc agcttttcac cccgttcatt ttctgaaaac acaattttcc
     4441 ttatctgacc ctcattattc gcaattattg agttggccgc attaactatc gtctgggtgg
     4501 acctgtaatt ctgctcgagt ttataaagct tataatctgg ataatcgttc ttgaattcca
     4561 ggatgttttc aatccttgcg cccctgaatg aataaatact ctgtgcatca tccccgacaa
     4621 cgcagatgtt tttatggcct tcagccagct ttctgattat aagatattgg gagtaattgg
     4681 tatcctgata ttcgtcaacc agaatgtact gaaacctgga ttggtacaat gccagtactt
     4741 ccggaaaatc cctcagaagg atattggtgt tcagaagcag gtcgtcaaaa tccattgcat
     4801 tggatttgaa gcatcttgct gcatagttcc tgtaaatatc tgccagcatc ggcattctgt
     4861 tcattttgtc atgttcctgt aaggaactgt tggctgaata catgctggcc gtgataagat
     4921 tgttcttggc ggttgatatc cgggctgata tcatacccgc cttgtaaata ttgtcatcga
     4981 ggttcagttc cctgataatg gtgcgtacca ggcttttgga atcgctggaa tcatatattg
     5041 taaaatttgg cttgtaccct agccgggggc cttccgtgcg caatatcctg gcaaaaattg
     5101 aatggaatgt tcccatccag agatgacggg tgatatcggg gccggcgatc ctggtgatcc
     5161 tgtctttcat ttcccttgct gccttattgg taaacgtcag ggccaggatc ctgtgtgcgg
     5221 gaataccctt ctgcagcaga tgcactatcc tgtaagtcag gaccctggtc tttccggctc
     5281 cggcacctgc aatgaccaat gccggtcctt cagtattgac aactgcctgc ctttgagctt
     5341 cgttcagttc tttaagatag ttttgcgtca tcggtcgaac gggcatattt aagcctaaca
     5401 ggtgcggcaa atttaacaat aaggggttca acggtgacgg gggcggttgc taatttatca
     5461 acaatacttt tcagtgagtt aaaaaatgct tgtaaacggg tacccggttc tgttttaaat
     5521 acaatgcggt tacattggtc cgacaggttg aaccgacaat ataaacagat tgcattgcca
     5581 acatattttg atgctaaaac agaaaaaaca aggctgtatc ggaatacctc cgatacagcc
     5641 tcattggaac ttagcttact cctgcataat tattttttgc ctgttttcgg ctttgtctcc
     5701 ggcttatgat gagccatggg agaacttccg gctgtgtgtt tttcaggaga gcccgatttt
     5761 agagccattt tatgcatgaa ctttccatcc ttgtcaaaaa caagggtttc ggaatttgaa
     5821 ccttttgtaa tcacaacttc aaacgttgtg acattgtttg caacgacttt atcggcttta
     5881 gttattgcaa agcctgcata atctttggtt atccagtcgg ttattgattt ctgaagatcc
     5941 gctgttttga ctgcggtacg ctgtgcgtcc accgtcattg ccaacacagc cagcaccagt
     6001 atgaacatta attttttcat tttttgaaaa ttttggttta acggattgaa attattatcc
     6061 gattctgtag tggaactgaa gctgaggaga ttcaggagat attttaacaa ttatcttaca
     6121 ctatgatttc agatcccggc aggagatgca aaaaggagtg ttatttcatg tacctttccc
     6181 acacatgtgt agacaatttt catactgtat gcttcaacta tctttttaac gattgacagt
     6241 ccaagtccaa cagattgcgg attgtcacct gctttttgga accgtctgaa taaaatcgcg
     6301 gtatctgttt ttaatggagg gccgctgttt gagattatga gcttgtcctt gtgcacctgg
     6361 cacctgatga acccgccgtc gaaattatat ctgacggcat tgctgatgag gttcgatacc
     6421 agagtgtcag ccagcatttc gttcattctt accatggcct tgtctccagc caggaaatct
     6481 acttttattt tcctgagatt catgatctcc ctgtaaccaa gcaatatctt ttcaattgtc
     6541 tctgcaagcg agatatcagt ttcgtcctga aaatacatat tatcaatttt cgagatcatc
     6601 agcaggccct ggttgatctt gtacaatctg gttgcagctt cattgatcga caggataagg
     6661 ttcagactct cttttctcag ttcctttctc tgggcaagga tttccagctt tgatctgatt
     6721 acagccagtg gagtctgtat ttcatgtgcg gcattttcat gatattcttt aaggttcagg
     6781 taatcatccc tcattttttt tgtcatctgg ccgattacag cattcagacg ttcgaattca
     6841 ctgatattgg tttggggcag gacaggaagc ctgtccgatt gaatgtcgaa tttctccgcc
     6901 tgatcgacag tgatatagaa agggcgccag agtcgtctgg aaaaaagata attaagaaag
     6961 attgaaatga aaaataatga aaggaaaagg aaaaatatat aaacaccaat atattccaga
     7021 agtactattt tctccctgag cgtttgggat ataactacgg aaaaaccggt attttcatcg
     7081 aggtttcctc tatagagtat atatctgtaa ggatgttcat atcctgttgt atccgtaatg
     7141 attgtatcat tgatgacctg aaccggacgc acctttccgg atatcagccg tacctcaagc
     7201 tggtggcccg gcctggcact gaaatccggt attgattttg tctgctctat ctgatccctg
     7261 ataatctcca gctcagtcag cagcgaatta tctatctggt cataaaccat ctttctggtt
     7321 gaaaaataca agagcacccc accaagcatg atcaggatgc cggaatatgc tagaaaatag
     7381 acagtacttt tagtcagcag tttcacggat caccgaattt gtatccaaag ccgtaaatgc
     7441 accttatata attcctgcca ccttccgttt tgattttttt tcgcaggttc ttgacgtgag
     7501 tgtatacaaa gtcgaatgaa tctgtcaaaa tcacatcgtc gccccagagg tgttccacga
     7561 tggattcctt tgttattacc ctgttcctgt tgattatgaa gaaaagcagc agatcatact
     7621 ctttttttgt cagattaagc tgcctgttgt tgacaaaagc ctggttgtca gaggttctta
     7681 tcctgagctc ttcaaatacg acctcattct ggcccgacag gttatttctg cggtagattg
     7741 aattgatcct tgcgcttagt tcagcaagat gaaatggttt tgtaagataa tcatcagcac
     7801 cggtcctgag gcccgtgagc ttatcctcca gtgaatccct ggcagaaagt atgagtattc
     7861 cgtcagatac cttgttttcc ttcagttctt taattatgtc aagcccgctt ctgtcaggta
     7921 ggccgatatc caggataatg caatcatacc tgttgtcggc gactttaaac agggcctgtt
     7981 tgaaatccct tgccagctca cagatatgtc cctcagcccg gagatagtca ttaatggctg
     8041 cactgagaga attatcgtcc tcaacaatca gtattttcaa accgtttgaa atttctgcaa
     8101 aattattaat tccctgaaat ccggattttc aggagtcgga aaaatattct aaatacggaa
     8161 gccattaaaa ccgacataaa aaatcaggtg aataaatatt cacctgattg taaacaaagt
     8221 tcatgtgatt cctgctctca tgctttatca ttgagcctga atgaaacggt tttcttcaag
     8281 ccgtttttct caaccgtgat cctgaaatgc tcctcaatat cctgcggaga attatagtat
     8341 ttaataactt ccctgtctcc tacaattttc catccggcat acttgctttt tgccagcttc
     8401 tcggcaacaa ggtggggaag ttcagcattt ttcctgataa tcctcgattg aacgatggtg
     8461 ccgtcaggat tataaatagc atatatttca ctgccatcgt ctgcctttat ataagcctca
     8521 tacctgtcgg gtttctggtc acctgccgat ttggaaacaa tccagccata tttctcaaga
     8581 acatatggga acttgcccca tttgaatgct ttaccggatt tgaaagtctc agtgacggcc
     8641 tgtactatcg cggcaggtat ttgttccttt tttaaaatga gattctcaac gggaatttca
     8701 tcgttttcac cctgatcata atctacctgg gcaagtaatg ggaaaatgaa tgcggtgaat
     8761 ccaattagtg ataaaattaa ttttttcata acttcagagt cttttggtta ataactgggt
     8821 cgaaagtatt tttgaattct gaagagattt tgaagacact gctgatttaa caaaacttta
     8881 ggacatcatt ggttttaaag ggcaataatt ggacctggca gtgaagatcc ctgctaatgt
     8941 atgccgggtt actatcccgg attgagttgc agctggttaa ttgaaaaaat aataatacaa
     9001 tgacgaaatt tctcccgctt ttgttttttt tgatcgtgct tgattcctgt tccggaagga
     9061 atatgcagcg acgtgaactg aggatatggt tcgatcatcc tgccgatgca ttggttcctg
     9121 atgtggaaga tggctggcgg aatgatgctg aatggctaaa ggcgcttcct gtcggaaacg
     9181 ggttcatcgg agccatggtg tttggagatg ttaataccga gaggatccag ctgaataata
     9241 aaaacctgtg gtcgggaagc cccgatgaca acgataatcc tgaggcatct gaacatcttt
     9301 acagaatcag gcgcttgttg tttgaaggca ggtatcgcga agcaactgaa cttactctca
     9361 ggacacaggt ttgcagggga gccgggtctg gccacgccaa tggagccggt ttacctttcg
     9421 gctgctacca gaccctgggc gacctttggc tcgattttaa cagggatcag ggttacgaaa
     9481 aataccatcg cgagctgaac ctcgatcatg gtgtggtttc agtcagctat acgcaggatg
     9541 ggataaaata taaacgggag gtttttgcca gctatcccga tcgtgcactt gtgatacaca
     9601 tatcatctga tacccggggt tccgtgacat ttgattcatg gtttacccgg cctgaaagat
     9661 cgcggaccag ctcgtctgat ggccatattc taatgagcgg cactctctcc gacggcaaag
     9721 gcggtgaagg aatgagctat gccgtgaggg taaaggccgt tgctgccgga ggaaccgtta
     9781 cctgttcgga tggtagaatc agtgtaaaca aagccgacga agcgcttctg gtacttgtat
     9841 cttcaacgga ttataagcag gaatatccgg attatcttga cggagatccc gtaactattt
     9901 cactggaaca gcttaatgag gcgtccttcc ggcctttcgg ggaactgttg aagcggcata
     9961 ttgctgacta ccgctcgctg tttggaaggg tgagccttga tcttgcgggc agggagaagg
    10021 ataccgtgcc aaccgacagg agattgctga atcagcttga agatcctgat gatatgatcc
    10081 tccatgaaac ctattttcag ttcgggagat atctgcttat ttcttcgtca cgcgaaggtt
    10141 ccctgcctgc aaatcttcag ggtatctggg caaacaagat acaaacaccc tggaactgcg
    10201 attaccatac taatatcaat gtgcaaatga actactggcc tgcggatgtg ggaaatctgt
    10261 cagaatgcta ttctcctctt tcagacctta tcctttcact ggttaaaccc ggagaaagaa
    10321 ccgccgcggt tcaatacgga gcaggcggat ggtgtgtgca tcccataaca aatgtttggg
    10381 gattcacagc tccgggagag catcccggat ggggcctcca tgtagttgct ggaggctggc
    10441 tgtgccagca tctttgggat catttcacat ttaccctcga tactgcttac ctcagaaaag
    10501 tttatccggt gatgctgggc tcagcccgtt tttatctcga ttggcttgtt gaagatcctg
    10561 tttcggggga gctcgtttcg ggaccggcag gctcaccaga gaattcattc atcgcacctg
    10621 atggctcaac ggcacaaata agtatgggtc cctctcatga tcaggaagtt atctacgact
    10681 tgttttccaa cgtcctgaac gcatcggcag tgttgaatga ttctgacccc ctgcttgccg
    10741 taataaaagc cgcccgcagc aggcttgcaa tgccgcaaat aggatcagat ggccgcctta
    10801 tggaatggag ggaagaattc agggaagcag aaccttttca ccggcacgtg tctcacctct
    10861 atatgcttca ccccgggaat cttatcgacc cggtcactac acctcagctt gccgatgctg
    10921 caaggaaaag cctggaagcc agaaccgata ttggtaccgg ttggtcactg gcatggaaaa
    10981 taaatttctg ggccagactc cataacggga acagggctta taaacttttg aaaaacctgc
    11041 taaggccaat taccacttat tcggttaata tgtcggatgc cggaggtaca tatcaaaacc
    11101 tgttttgtgg tcatcctccc ttccagatag acggaaattt cggagcaact gcaggaattg
    11161 cggaaatgtt gcttcaaagc catacaaagg aaggtgaaag gtatgtgatc caggtgcttc
    11221 ctgcattgcc cgatgcctgg aaagacggtg aggtaagggg actgaaagcg aggggaggtt
    11281 tcgaggccga cattaaatgg aagaccggta aactggtatc atgtacaatc cggtccaatg
    11341 ccggagccgg acttaaaatc tgctatggcc ccagatcggt cgtgatagac tccaaaccgg
    11401 gaatgacgta tcactttaac ggtaatcttg agtgattcag cctgattatg acagatcgtt
    11461 ctgatatgat aacctgagcc gtctgccggg aaaatcatgc tttgccagga ttttgaacct
    11521 ggatcctgcc gccggaacag tgatcgtgat tggccttgta tccaaataat gaaataaaga
    11581 tccttgtaat ataattatcc gaataaatca gggttttatt atacttgcag gattattgat
    11641 accaaaaaag ccattatttc gtttttgcta cgaagatctg aatatccgtg cgttattatt
    11701 tatttaccgt tgctatattg ttgacagtca ccctggaagc ccagctcact gcaccgggca
    11761 gcaatgctgt caggtatact tcctatccgt cagctccttc gctgagacac ccgatattta
    11821 ttttctgtaa cgcctcagga aatcagaaag gagaattgaa tgcagtgagc cccggcggaa
    11881 ccggaccttt cactttttcc tggtccaggt ggaacgatgc gacacggaat tttacaatcc
    11941 cggttaagaa tgaaacaggg gttttcagtt cttcagttac aaacctcgat gaggggggat
    12001 accgggtcaa cataaccggt gcttccggat attctgccag cctgacaggc tggatatttc
    12061 tggactcacc ccattccaaa gccgaacttc gtcattttac ttgtgattat gtggcgctgg
    12121 gaggagaggc agccgttgat acattctact accgcgacat caacagcggc tcacggatca
    12181 ttctgcccaa cagggtcaga ttcctctggt cgtcccagcc ggcttcctcc attccatatc
    12241 ccgacattga acttaacccc cagacattca atccccccct ggatgatgtc acatacaacc
    12301 tgcatgtgac cgacagcttc tcctgtacaa ccgaatcatc cttcttttat gaatccattc
    12361 acgttaaggc tgacttcact gttgatccag ctaaaggcga ggcccccctt gaagtcacat
    12421 ttaccgacaa atcggtaaga gccttgaagt ataaatggat ttttggcgac gatacatttt
    12481 ccaatgatat cagtccagcc ccacacacct actttatccc gggggattat aaggtcaggc
    12541 ttattgtcga aagctcgttg cattgtattg attcgatgac attcgataaa attgttgtcg
    12601 acaaatccgg cctcagtatt ccgaatgtgt tcacgcccaa tgacgacgga atgaatgata
    12661 tgtttatggt tgacaaaact tcactgagat acataagtgt cgatatatat tccaggagcg
    12721 ggataaaagt ctatgagttt tacggcgaag gagagatatt aaagaactgg gaaggatgga
    12781 acggaaatat aagaggaact tcgcttaagg caagccctgg agtttattat tatattataa
    12841 aagccagggg atgggacaat gtcaggtatg acaacaaaga gtacagggga ttcgtctatc
    12901 tctacagatg atcctgttga tgaattgcct gtttcgggta gatatactaa aggttgattt
    12961 ttcgtttcct ggcctcaacc tgccactgcc cggttaccat ccccccttct gatacatata
    13021 ctgtatcata acgatctact gttggacaga tatgccatgg aattgcgtag agatgatcgc
    13081 cgacgtttat ttttcctgct tccgtggttt caattaccat gtgttcttca ctgtggccgg
    13141 tgacccggta atcatgcatt ccttcaatat gtattctcgg gtggggcatc tctgatgcaa
    13201 cggccttgtg tcccagatcg atgcatatct tgttgccgga aggtttgctt ataacccttg
    13261 tcagaagaac ggcagcataa aggaattgca tgtcgctgaa tgatgagccg taaccataat
    13321 cccacagcag ggttgtcccc gggcttaatt cggtgttctc cctgcgggcg tgaaccggaa
    13381 atgtgggggt gcctccggca ataaccttta caggcgctcc tgtcataatt gagatatctt
    13441 caatcagtga ccggaccggt gcaaatgcct catcacatat ttttatcctg ttttcgacat
    13501 ttctttcgtt gatatggcca tcataaacat gcagacctgc aggtacaagc ccgggcagca
    13561 atgagatcat cctgaaaagc tcccgcgcct tttcccccgg ttctatgccg gtcctgttca
    13621 ttcccgcatt gatgtcgagc catatatgag ccttataccc ggtgactgat gcatgatccg
    13681 acaactccct gaggatagac tcacaatcag atatgcatga aaacttcttt gaaggaaatt
    13741 tccttgggag cgaaaaaaat ctctgaatat tgggtcctac aggctggtaa gccagcaaaa
    13801 tatcatcggc tccggccctg gctgccattt cggtttcggc gattgtggag catttcacct
    13861 tcctgattcc gtgcctgacc tggagttgga caatttccgg aattttatgg gtcttgacgt
    13921 gcggacgaag cctgtccgcg ctgcccgcca catcaatcat tttcctgatg ttggcttcta
    13981 tcctttcagg ataaaggatc agagcaggag tttcaagttc tcctgcattt cctgccacat
    14041 accaccctgt aaatggtgat cctggggatt gcatattacc ggtgtatttc gaaaacggcc
    14101 tctatttcca ctgcgatatt cattggaagg atcatgccga cagcacttct gacgccaatc
    14161 ccgttttcgc tgccaaacac atcggccatc agttcactga atccgttgat cacggcagga
    14221 tgctgatcaa aatcctgggt actgtttacc ataccaagta cttttacgat cctcctgatc
    14281 ctggaaagat cgccgaaatg tttcctgatg gtggcaagca tggccaatcc aacctgccgt
    14341 gccgcaagct ttccgtcttc aagcccgaga tccgcgccca gcctgccttt aatcagtgtt
    14401 ccgtcggatc tgagcggtgc ctgccctgat acatacagta aattctctac gacaagcact
    14461 ggtttgtata ctccggcagg atcgggggca ggaggcagaa taagccctaa ttctgccagc
    14521 cttgattcag ggttgatttc catttctatg atttttgcga ttataaatga tgattcgttg
    14581 ttccgctatg ataaaataag taaaaatccg gcaaatatac gaaagacaat tctagatgag
    14641 caggtctgca accagtaccc caagaagccc gatgacacta actattgtct ccatgaccgt
    14701 ccaggtttta aatgtgtctt ttacgcttaa gttgaagtat tccttgtaca tccagaaacc
    14761 gctgtcattt atgtgtgaaa gcatcaggct gcctgctcca atggaaagaa ccaggagttc
    14821 tttgtttacc gttcctccag tagccagcgg cagaaggatc cctgccgttg tcaggccggc
    14881 aacggttgcg gaaccaatgc atatcctgat cactgctgca atgagccacg caagcagaag
    14941 cggtgacatg gaagatttgg tcagtaaacc ggcaatgtcg tcactaacac cgctgcttac
    15001 aagaacctgc ttgaatgcac ccgctcctgc aatgataaga agtaccattg taatgctgcc
    15061 tatcgaattc ccgagagaat tcattatgtc cgtcatactt tttccccgcc tggttccgag
    15121 aaggtatacc gacaggatca gggagatcag cattgccata acaggatcgc ctataaattc
    15181 cagaaccgat cttaacctgt ttccttcggt gagaaatatt gtggaaacag aataggctcc
    15241 tataagaatc accggcatca gggttgttaa cacgctgata ccgataccgg gcatctcatc
    15301 atcactcatt gccctggagc tgacaaattc cttaatgggg ctcgccttga tctttttcag
    15361 ggtttttccg aaaaacggac cggcaacgat aatagccgga atgcttataa taatgccaaa
    15421 accaattgtt ttgccgatat cagcgccaaa catcgtacat atggcaatgg gggaaggatg
    15481 aggcggaagg aatccgtgag tgacggaaag cgatgccagc aatggtattg ctacgtaaag
    15541 cagcggaaga ccagttgatg ccgcaacggt aaaaacaagc ggaataagta caacgaatcc
    15601 tacattgtaa aacattggga tgcccaccat gaaaccagta agtaccattg cccactggat
    15661 gttcccgata ccgaacagat cagtaagttt tgtggatatt cgttgggcag cgccgctgtc
    15721 ggcaaccatc ttgcctagca ttgcaccgaa accgagaatc aataccagcg accccagcgt
    15781 gtcgccgatg ccctgctgaa tggaagtaat aacatcgatc gccttcattc cctcggcgac
    15841 tcccacaaat aaagaagtaa ttacaaaggc aatgaacgga ttgtatttga gaacaataat
    15901 aagcagaacc agaaacagaa taccgataac tacccaaaaa attgacatct gaccaggctt
    15961 ttatttatag tatttcaggc tgtataaaaa tgtcgtggag cagaaaattg cagaggatga
    16021 gctagttaac ctgttcagga tcataaaaaa tgaattttta cagtttgttc gggcttattt
    16081 gagatttctt cctgcacata gtccgggata tcatacattc agactttaat tccatgtctc
    16141 aatcgtcatt tacccgtaaa tatcgctttt ttaccgaatt taaacggaat ttaacaaata
    16201 aaactatgaa aactaaaata gtttattcat ttttgcacga tttattttca ggcatggatt
    16261 acaggcaaag aattacggaa gaggctgcgc gtctgttcag acagtacggt attaaagcag
    16321 tcacaatgga catgctggca aataatcttg gcatatccaa aaggacaata tacgaagttt
    16381 tcaaggataa gaatgagctt ctggcgggtg ttgtcagtca tatggtaata cgacagaagg
    16441 aattgattaa caggattttc aatgaatcag agaacgtgat tgaggccata ttcaggctcc
    16501 tggaaacgat gagtgagcat atccggagaa ttagccccgc attcaggctc gacatgatga
    16561 aatttagcaa tgatgccatt atggatctga aaaaaacggg tcatttccct tatccttatt
    16621 caaccccgta cctgatagaa aatggaatta aggaaggtgt tttccgcgat gacattgatg
    16681 ttgaaattac aaataaatgc ctgtttgaat tgatccggat gtcagacagg ggtgatgatc
    16741 ccgagcagga tgccaatcac aggatgcacg tgatcaggga tgtttatata aattatttga
    16801 ggggaatctc gacccccaag ggccttgaac ttataaacta ctatgaaacc cacatgattg
    16861 accagaagcc gagacacgaa tgagtattac tgcgttcaat cgtgagtggt tccctgcccg
    16921 tgaggcatac aggcatttga tctttaaaca ataaattata aaaggatgaa acccatatga
    16981 tacagaatca caaacaagca tgaaaagaag attcatgtgg gttccatgtg aaccacttaa
    17041 tctccagtag tatataaatt aattttcaca tgataagaag gaaagtgata ttaaagcaaa
    17101 ccagccgtcg gaataaaatc cgggtcatgc acgcaactat cttttattcc ggcagcatct
    17161 tcaccggatg tacaatcatg tttgactgaa agctttccgc aatttaatta aagaggcgaa
    17221 tttatgaaaa gaagactggt aatcatactg atactatccg gctttctgca cggttattcc
    17281 cagcaagccc gggagggagg aagcgaattg aagctttcat taagggatgc tcaggattat
    17341 gccatcctga acaacaaatc agttctggct gcaagggccg atgtcatggc ctcgaaagct
    17401 gctgtgtggg aaaccatatc agccgcactt ccccagttaa atgctaacgg aacatttgca
    17461 gacaacctca agctgatgac aacccttctg ccgggggagt ttttcgggca gccgggtaca
    17521 aaaattccgg taacattcgg atcgaaatat aactcaagcg cttcctttca ggcaaacctg
    17581 ctgattttca atgcgcccct cttcctggga attgaaacat caaaacttgc ctacaaactc
    17641 aacgcgaata atctcgagct ttctgaactg gacaccaagg aagctgtcag ttcagcatat
    17701 tttctcatac ttgtatcaga aaaatccctc gaaatcctta atgccaacat tgccaacctg
    17761 aatgaaacgc tgagatcaac aaggtcaatg tattccgcag gtatggccga atcgactgat
    17821 gtcgatcaga tggtttccaa cgtgacaatg atggacaata acaggagttc actgttgcgg
    17881 acaattgaac taaattataa cctgctccgt tttcagctgg gtgtttcggc cgataccagg
    17941 atcatactga ccgacaatct tgaaggactt acaaatgccg ttaatgtgga agcccttatg
    18001 tctcaggatt ttgatcacac aaagaacatt aattacaagc ttgttgaagg tcaggaactt
    18061 atgtcggaac tggctcttaa atctcaaaaa gcgtcagttc ttcccacact ggctgggttc
    18121 tacaactatg ggaccagcgg gatgggcgac aaaatgaggg atctaagctg gtttcccaac
    18181 tcaatggccg gactgcaggt atcagttccc atttttgcaa gcggcgaacg ctatgccaaa
    18241 atacaaaagg ctaaatataa cctgaacaaa gccaggataa ccaggaatat gattgaagat
    18301 cagcttctta ttcaggagaa gcagctcagg tacaatcttg tgaatgcaaa ccttcagtat
    18361 cagagtcaga aggagaatgt ggatgttctg gaacgggttt acaaaagtac ggagaataaa
    18421 tacatgcagg gcatggcctc cagtctggaa ctaacccagg caaacactca gtacctgcaa
    18481 gctgagaaca actatgtgtc cgctctgatg aatcttcttc tcacaaaact ggcattggat
    18541 aaacttctta ataatctgta aaaatcagtg aaatacataa attacaaaat aatgaaatac
    18601 ataaaaacaa tcccggtact ttttttaagc tgcatgctgg tggcagcatg ttcatccagg
    18661 aaaaaaagtg ataattcagg agctttggca gcaactgctt ccccagatag aaccgcggtt
    18721 cccgtgaaaa tcctgagcct ggctaaaagt acgatctcaa ggaccatcga ttatacggca
    18781 acaatacttc cttacgaaga ggtgaatgtc gttccgtcga ctccgggcag aatcgagaag
    18841 atcaatgtcg aagtggggga ccgagtttcc aaaggcgatg aactgtttct catggaccgg
    18901 acccagcttt accagcagaa gatccagctg ggaagcctgg caaaggatct tacccggctt
    18961 gacacacttc tgagaaccgg aagcattaaa cagcagcaat atgatcagct taaaacacaa
    19021 tatgaggtta cccgctcagc tgtcgaattc atggaggcaa acacgcatct gaaggcacca
    19081 ttcagtgggg ttgtgaccgg caagtatttt gaagacggtg aaatgtattc gggaacaccc
    19141 accacgcaat cgggccggtc cgcagttgtt acactgatgc aggttaatcc gctgaagata
    19201 gatgtgaata tttctgagca atactatccc ctcataaaaa aaggcatgaa agccagtgta
    19261 gttgctgatg tatatgagaa cgaagttttc acaggttcgg ttttcagaaa ggcgcctaca
    19321 ataaatgcag caaccagatc atttgtaaca gagattgaat tacccaacag gaatgatctt
    19381 ctcaagcccg gtatgttcgt aagggtatcc atggaccttg gagaggtaga ggcttttgtt
    19441 gtcccttcca atgccgtatt aatgcaggaa ggcaccaata tcagatatat ttttcttgaa
    19501 gaaaacggca gggcaaaacg cgtcgaggtg accctcggga aaagatttga cgataaactg
    19561 gaggttctat cggaattcct gaaggaagga accggccttg tcaccgaagg tcaggcaaag
    19621 cttatcgacg gtgacagaat tgaagtgata aaatagttat gcaccttaca tctgagataa
    19681 aatgagtatt tacagttctt cggtcaagag acctgttacc acgatcctga tttttgtggg
    19741 attgatggta atgggactct attcactggt taagctgccg gtcgatctgt atccggaaat
    19801 ggaacttccg tatgtggttg tctatacgac atatcccggt gcaagtgcct ctgatattga
    19861 gtcgaatgta acacgccccc ttgaaaattc acttaattcc gtcagtaagc tcaaggagat
    19921 tacatcacgg tcaagcgatg ccgtatctgc tattttcatg gaattcgagt acggaactga
    19981 tctggatgaa gcttcaaatg atatacgcag tgccataagc tttactgaga gactattgcc
    20041 agacggcagt gaaaaaccga ccatcgtcaa gttcaataca acgatgatgc ccataatgtt
    20101 ctacgccatt acagcaaggg aaagttatgc gggactcgag aaaatcctcg acgaaaaggt
    20161 ggtaaatcct ttgaacagga ttgaaggtgt cggcgcagtt tccttttcag gagtcccggg
    20221 caggaaaata tatattgatg tagatccccg gaaaatggag gcctataatc ttaccatcga
    20281 acagattggg aatatactgc gggctgaaaa tatgaatctt cccgtcggtt atatcgagat
    20341 gggcaatact gattatcctc tgaggatcca gggcgaattc ccggaaagtg atgtggttaa
    20401 gaatattgtc gtcagcagct atggcggcag caatatttat ctgaaagata ttgctgaagt
    20461 ccgcgatacg atacgggagt caaaacttga cacgaaaatc aatggtgtta agggaatggg
    20521 actgctggtc cagaaacaat ccggaggcaa cacggtccag gttacgagga aaattgaaaa
    20581 atcacttgtc cgccttgaaa aatcccttcc tcccgacgtg aaaatcacca agctttttga
    20641 cagcgcttcc ttcatcaggg attccattag caatcttacg gaaacactga tgtatgctgc
    20701 gatatttgtt attctggtgg tcctgttctt tctcggaagg tggagagcca ctttcataat
    20761 tatcctgaca atacccattt cccttgttgt ggccttcatc tatctgtttg tttccgacgc
    20821 atctataaat ataatatcgc ttacctcact ttccatagca attggcatgg tggtcgatga
    20881 tgccattgtc gtactcgaaa atatcactaa gcatattgaa agagggagtc gtccccggga
    20941 agccgcaatt tatgccacca atgaagtttg gctggcggtt attgtcacta ccgcaactgt
    21001 ggtggcagtg ttttttccgc tgactttcgt ttcaggactc accggggtgc ttttcaaaca
    21061 gcttggcatg agcgtgacaa taacgattgt aacatcggtt tttgcagcaa ttaccctgac
    21121 accaaccctc agtgcccttc ttttaaaatg gtatccgata aaggaggatg cgcctttctg
    21181 gacctacgac ggaagcatta ggaaaagcct tgaccgcttt gaccggtttt atgaaaaaac
    21241 attgaaatgg gcactacggc acaaaacatt tgtggttata gtatcgctgc tggtttttat
    21301 cggttcaatg agcctgtttt tctttataaa gacagaattc ttccctcagg ccgatgaatc
    21361 gcggattact gcaaaagtag aattgcagac cgggacaaga gttgatcaga caattcgcac
    21421 tgctgacagg ctcgattcac tcatcagggc aaaatacccg gaggtcagac tgatatcaac
    21481 ttctgccggg gcagacgacc agggaggatt tgcagctatg ttttccaacc aggggacaca
    21541 tacaattaca tacagtatcc ggcttgttcc cgtaaaggaa agaaagagat catccatgga
    21601 aattgcagat gagttaagaa ctgactttgc aaaatatcct gaaatcattc attataccgt
    21661 cagcacgtca gacaatatgg gaagctttgg cggaagtacc gttgatgtgg aggtttacgg
    21721 atatgatata gcgcagacga atattgttgc tgaagacctt gcagcacgga ttaaaaatat
    21781 caggggggca aaggacgtca ctgtcagtcg cgacaaatcc aaacccgaac ttcagatagt
    21841 ttttgatcag aacaaaatga tggctaacgg cctgaatacg gcaatggcat ctgctgccat
    21901 cagaaacagg atcgatggac tggttgcaac aagactcaga cagtacggag aggaatacga
    21961 cgttgtggta agatttaaga gaagcagtac cagcactttg accgatattg aaaatattgg
    22021 gataaccaat gcaaccggcc agctgatcag acttgctgaa atagcacgga ttaaggaata
    22081 ctgggcgcct cccagtatag aacataaaag gaaagaacga attgtgacag tatcatttac
    22141 ccctttcaag cgttcactca gcgaattgaa ggtggaggtt gaaaaagcga taaattccat
    22201 caatatgccg gccggagtaa tggttcagat ttcaggctcc atcgaggaac agagagaatc
    22261 attcatggac atcggtttcc tgctggtgat cagtcttata ctcgtgtacc tcataatggc
    22321 ttcacagttc gaatcactca aaatgccgtt tataatcatg ttctccatcc cgtttgcctt
    22381 ctccggagta gcaatagcat tgtttgtaac gaataccgca ctcagcgtta tttcagcaat
    22441 cggagccgtg atgcttatcg gaatagtcgt gaaaaatgcc attgtacttg tcgacttcat
    22501 caatcttatg agggaaaggg gtaatgaact ctatgaggcc attgcaatat caggccgctc
    22561 aaggctcaga ccggtgttta tgacatccct gacaacaatt ttcgggatgt ttccccttgc
    22621 tgtaagcagt ggtaccggtt cagaactctg gagcccgatg ggaatatccg ttataggagg
    22681 acttgtattt tcaacaatcg ttaccctggt gctggtcccg gttgtgtatg caatatttgc
    22741 aaagcatggg gaacggaaca agaatctggc catatacatg gaaatgaatt tcatgaatga
    22801 taacggtaat gaagccggcg acacatttac tgataaatga ccgttatgtg acaatttaaa
    22861 tctaaattat ttcccttatg aaagcggtaa tgataatata caaccaggca cattccgaga
    22921 aaattgaatt tcttcttgac aaggttggcg taaaagggta taccctttgg gaaaatgtta
    22981 aaggaagagg ctcagatacc ggagttccgc atttcgggac ccatacatgg cctgaaataa
    23041 ataaaagcgt tctggctatc gttgacgacc cgatggtgga tgagatactt gataaggtaa
    23101 aaaaaattga tgccataaac tatgatgtcg gtatcagagc ctttgtgtgg gatatcctta
    23161 aaactgcctg aatgcagatc tgaccggatc aaaaagaaca gcatggaata catcgaagca
    23221 tgtttatccc aaaatccgcc cgactcggga aaaggatgaa gcccagataa gtacaacata
    23281 aggctaaaaa gatgaaaata tatcacaaac taccagggca aacgaatacg atttcggatt
    23341 tgtttatccg gcccctatag gattttaatt aaaagagtgt ttttattata caaaaaccaa
    23401 ttatatttgc tatatacata tcatttgtta cagtttggcg gaccttgcta tataattgtt
    23461 aaaataattg tgattttcag atctttcatg atctgataca ggttctgata tataggctat
    23521 tgacttattt ccggtaataa tgaaatatga tgtagtgatt gtgggaggag gtgtgagtgg
    23581 aacagcattg ctgtacacac ttgcacgata tacagatatc aggaatattg ccattattga
    23641 aaaatgctgt gacttcggcc tgattaattc ttcatcacga atgaacagcc agacattgca
    23701 tttcggggat attgaaacca actattccct cgaaaaagcc aaaagggtaa aaaggtcggc
    23761 cgacatggtg atgaactttc ttgaaactga aaaagccgta aatggtgacg agagactttt
    23821 tgtcaaggtt cctaagatga ttttagctgt cgggaaagaa gaaacgggga agttacgggc
    23881 aagatatgag gagatcaggg agctttttcc acggctcaga atgatcggga aggaggatat
    23941 tgccagactt gaaccccgga ttgtggttgg acgcgatcct gaccaggatc tgctggctct
    24001 tttcacaagg gatggttata ccgtggattt cgggaagctt agtcatgctt ttgttgataa
    24061 ggcaaggcag gaaaatcctg atatatcgat taatctttca acgagagtat taaaagttgt
    24121 caggaatggt aatgagtatg atatcctgac cgataaaggc agctttaaat cggaaacggt
    24181 tgttgttgca gcaggtgctc acagcctcct gcttgccaaa tccctgggct atgggaaaaa
    24241 ctatacaatc ctttcaatgg ccggaagctt ttatttcgga cccgctgtcc tgggcggaaa
    24301 agtttataca atgcaggagc ctaaactccc ttttgcagct atccatgggg atccggaagt
    24361 tcacgacaga aacaccacac gattcggacc caccgcaaag ccgattctat tactggaacg
    24421 gtacaatgct tcaaccttta ccgggtattt gaaaacctgc ggattgaata tcagatccct
    24481 tatcagtctc gtgaaaattg cttccgacaa aatcgtcttc aggtttctgc tcaagaattt
    24541 aatttatgat atcccggcga tagggaaaaa agctttcctg aaagagatcc ggaaaatcgt
    24601 tccgggaatc agtggcgatg aagtaaaatt tgcacggaaa atcggaggaa cccggccaca
    24661 ggtagtgaat atcaatacag ggaagctgga gatgggagag gcaaaaatcc ggggcgaaaa
    24721 aattattttt aacgtaacac cctccccggg agcatccaca tgccttggaa atgcttttga
    24781 agatacactg ttactgatgg gttttctgga taataaatat aaatttgaaa gggaattatt
    24841 cgaaaaggaa ttgatcaaca ggaacgaaga tgtaactgac cagatacaaa gagagaattt
    24901 gatcaattcc ccggttgtat aaaagagtgg ttttaaaccg tttaaaaccg gagaatgaaa
    24961 gcgggatttt aaaaatatgg ataacagtta ctataaagat ttaaaattat tctaccggaa
    25021 cctgccggat aaaatatcga cgatcaggaa taacctgcag cagcctctga ctcttagtga
    25081 aaaaattctt tacagtcact tgtttgaaag taaaagctta gttattcaaa aacgtggggt
    25141 tgattatgtt gaatttgctc cggaccgtgt tgccatgcag gatgcaaccg cacagatggc
    25201 cctgctgcag tttatgatgg ccgggaggaa caagtccgcg gttcctgcgt cggttcattg
    25261 tgaccatctg attgtagccc gttcgggtga tgttgaagac ctgaagaatg cccggataac
    25321 aaacagggaa gtatatgact tcctgagctc tgcatgtttg aaatacgaaa tagatttttg
    25381 ggaaccggga gcaggaataa ttcaccagac catacttgaa aactatgctt tccccggggg
    25441 aatgatgata ggtaccgatt cacatactgt aaatgcagga ggcatgggta tgattgcaat
    25501 cggagtggga ggtgccgatg ctgtggatgt gatgtcgggg atgggatggg agctgaagtt
    25561 tccaaggatt atcggggtca ggctcagtgg cagactaaac gggtggacct cggccaagga
    25621 tgtgattctt aaactggctg gaatactgac ggttaaaggg ggaaccggtt gcattattga
    25681 atatttcggc gaaggctccg gatcgctctc ctgtaccggg aaagccacta tttgcaatat
    25741 gggtgccgag gttggtgcca cctgttcgat ctttggcttt gatgagaaaa ttgcttcata
    25801 ccttgctgca acaggaagga aggaaatggc tgatatggct tatgccttga aggaaaacct
    25861 tattgctgat cctgaagtct gttcagatcc tgaaaaatat tatgatcagg ttatcgacat
    25921 tgatctttcg gaattggaac cctacatcaa cggacctttc actcctgacc tggcaactcc
    25981 cgtttcaaaa atgaaagaag ccgcaaggaa tgccggctgg ccggtgaagg tggatgtggg
    26041 cttaatcgga tcatgtacaa attcctcata tgaagatata tcccgggcag cttcaattgc
    26101 cagggacgcc cttgacaaaa agctgtcgct caaatccgaa tttaccatca ccccgggttc
    26161 ggaacagatc agggcaataa ccgaaagaga cggatacttc gacattctcc ggaaaatagg
    26221 aggcagaatc tttgcaaacg cgtgtgggcc gtgcattgga cagtgggata gggaaggagc
    26281 aggaaagagc ggggtgaaca ctattgttca ttccttcaac aggaattttg ccaggcgaaa
    26341 tgacggaaat cccaataccc atgctttcgt tgcctctccg gaaatagtca ccgccctggc
    26401 tattgccggt gagctgacct ttaatcccct gaccgattac ctggtaaacg atgagggtgt
    26461 taaggtcagg cttaaagaac ccgaaggatt tgaaatacca atttccggat ttggtgaatc
    26521 cggcagaaat ctgcatgtgc aacctgcaga acgggaatca gtcgaaataa aaattgatcc
    26581 acagtccgat cgccttcagt atctgcagcc ttttgaaaaa tgggatggca gcgacgtcat
    26641 ggagcttgcg ctcctgatca gggtaaaggg caaatgtaca accgaccata tctcaatggc
    26701 aggattctgg ctcaaataca ggggacatct tgagaatatc tccgagaact atatgattgg
    26761 tgccttaaat tcctacaatg ataagacgaa cagcgttctg aaccagttga caggcaagta
    26821 tgaaacagtg ccgaaggcag ccaggcacta ccgggatatg aatatgggaa caattgtcgt
    26881 cggcgaggaa aatttcggtg aaggatcctc cagggaacat gcagccatgg agccacggtt
    26941 cctgggcgtc aaagcaatac tggtaaaatc ctttgcacgt attcatgaaa cgaatcttaa
    27001 gaaacagggg cttctcgccc tgacttttaa taataaggat gattatgata aaattcagga
    27061 atgggataaa atcaatattc tcggcttaaa gaaaatgagc ccgggtagac cgattgaact
    27121 gatactcaga catcgagacg ggtccaccga aaagatcctt gcaaatcatt cctataatga
    27181 agcacagatc gaatggttcc gggccggcag tgctctcaat cttataagaa tcaggaatca
    27241 ggataattca atttaaatgc tggtcctgaa aaaatgcgaa tatgactggt gaaattagaa
    27301 aagaaaaaaa caatttgatc gtcccggatt ttcctgttat tccgtttatc gaaggagacg
    27361 gaaccggtcc ggatatctgg aaagcctcag taagggtttt caacgctgca gttgaaaaag
    27421 tatacggctc tgaaaaaagg atagtctgga aagaagtcct agctggagaa aaggccttca
    27481 gggaaacggg caactggttg ccggaagaaa cactcagtac cttcagacat tatcttgtcg
    27541 ggatcaaggg ccccctgaca acacccgtgg gcgaagggat gcgatccctc aatgtggccc
    27601 tcaggcagga acttgatctt tatacatgtt acaggcctgt caggtatttc gacggagtac
    27661 ccagtcctgt caggcatccg gaaaaagtca atatgtatat tttcagggag aatactgagg
    27721 atatttatgc cgggattgaa tatttgaacg gtacccccga agcagaaaaa ataaggaaat
    27781 tcctttcaga tgaaatggga gtaaggaaaa taaggtttcc ggaatcatca tctattggaa
    27841 taaaaccggt ttcggtagag ggaaccgaac ggctggtcag gcaggcaata aagtttgcag
    27901 tcgacaggaa tcttccctcg gtaacccttg tacacaaggg caatatcatg aagtttacgg
    27961 agggtgcttt catgaaatgg ggttatgccc ttgctgaaag ggaattcagc aacgaggtgt
    28021 ttacgtggaa ccggtacagg gaaatagctg acagggaagg tgctgctcct gctgatgctg
    28081 cgctggcgga agcggtaagt aacaggaaag taataataaa ggatgtgatt gccgatgctt
    28141 ttcttcagca gatacttacg cgtcccgaag attattccgt aatcgctaca cttaatctga
    28201 acggcgatta tatttccgat gcgcttgctg caattgtcgg ggggatcggg atcgctccgg
    28261 gagcaaatat aaatttcgaa tcgggacatg ccatttttga agccactcat ggtacagctc
    28321 ctaaatatgc tgatcaggat aaggttaatc cagggtcggt aatcctctcg ggagctctga
    28381 tgtttgagtt catcggatgg aaagatgtgg ctgacaggat ttatgccgga ctggaaaaaa
    28441 caatcagaag cgggttggtt acatatgatt tccacaggct catgaataat gcaaatcttc
    28501 tgaagacatc tgagtttgca agtgcgataa taaataattt atgatacatt caaatcactt
    28561 aaatcaatat ccatgaccaa tgataaattc ttccccaggc tggaagattc tgttagggaa
    28621 tcatgtcaga ttgagaattc gttgtttgaa aaattcgatg taaaacgggg gcttcgtaac
    28681 actgatggaa caggcgtcct ggtcgggctg accaatatat gtgatgttgt tggctataaa
    28741 aaagaagacg gaaaagttat agccattccc ggaaaactgc tttacagggg gatcgaaata
    28801 gaggatatta ctgcaggctt tcagaaggaa ggaagacacg gcttcgatga aactgtatac
    28861 ctcctgctca caggaaacct tcctacatct gaagaactga aggatttctc ggcacatctg
    28921 gcctcgctca gatcattgcc tgatgatttt gtgaaagaca tgatcctgtc aatgaagggc
    28981 agagatgttc tcaacgttct cgccagatca gtgcttgggc tttataccct cgaccagaga
    29041 ccggatgata tttccctgcc gaatatgatc cgccagtcat tgaatctgat tgccaagttc
    29101 ccgacaatag tggcttattc ataccaggcg ctgagacatc tttacaaggg caaaactctt
    29161 tcgatacgcc atccccaacc taaacttact gtggctgaaa actttctgta tctgataaaa
    29221 ggggaaaaga aaggatattc aaagcttgaa tctgatcttc tcgatctttt gcttgttctt
    29281 catgccgaac atggaggagg aaataactcg acgtttacga tgcgggttac gagttcatcg
    29341 gaaacagata tctattcttc agttaccgca gcgatcggct ccctcaaggg tccattgcac
    29401 gggggagcca atgtgagggt tctcgaaatg atggagaaca ttaaatcaca tgtaaaagac
    29461 tggaaaaatg aagccgaagt ggaggcttac cttctgaaga tactcaataa gaaagctaat
    29521 gatcattccg ggaagatata cggaataggt catgccgttt atacaatctc agatcccagg
    29581 gccggattgc ttaaggcaaa agccagggaa ctggcaattg agaagggacg cctcgatgaa
    29641 tttgagctgt acgaaatggt agagcgcatc actcctgagg tcttccgggt tttcaagggt
    29701 gaaaagactg caaaggttgt ctgcatcaac gttgacttct attcgggatt tgtttattca
    29761 tgccttgaga tctcaaagga actttataca cctctttttg caattgccag ggttgcagga
    29821 tggtgtgcac acaggatcga agagctgact ttctcggcca agagactgat acggcctgct
    29881 ttcaagtacg tttacggaca gcatgaatat ataccaatgt ctgaccgcag gaaattttaa
    29941 gaacaaaaaa caatactaca tgaacacact acctgatgat cgttttcagc tggcatttga
    30001 taagatcctg gagggaaacc tgcaggatgc agccgggatc ctggaagaag agcatgttaa
    30061 aaatccgggt aatatagctg ttttacttga gctgggaaat atttattata tactctgtga
    30121 aatgtcaaag tctatagagt attataagaa aatccttgaa attaagcccc ttagtcctta
    30181 tgtaatgtac agggtgggtg tggcgctgta ccagtcaaca catttttccg aggctgcgga
    30241 ggttttcaac aggatcattg aatcggaaaa acatctgcca atgacttaca tgtggctcgg
    30301 actttgctat tatcatcttg gccaggaaga aaaaagcatt gcctcttaca ggaaattgat
    30361 tgaatacggg cctgaaaccg ttatggcaaa ttactatatg ggtgttgcaa tgaaatcatc
    30421 gggcaaatac cacgatgcaa taattcattt tgaaaaactc cttcaccaga ctgatcagca
    30481 tgtttctgca ctatatcacc tgggccggac atatatgaag gattatcagt ttgaaaaggc
    30541 caaggtatat ttcaaaatgg ctattgatct tgatcccgaa aacaagaatg cgtcagaaat
    30601 gtatgattat ctgataaacg gatgaggaat tcctattcat cctcctcttc ttcatgatag
    30661 gcttttataa ccgcatcctg aacatctcca ggcacttgtg catactccga gaatttcatg
    30721 gtgtacattg cccgtccgcc tgtaattgaa ctcagggcgg ttgagtattt attcatttct
    30781 gcaagcggga cccttgccct gacaacttca aagcgttttt cgctcgacat gcctagaacc
    30841 atgccgcgcc ttccctgcag atcgcttata acatcaccca tcctgtcagc cggaaccatt
    30901 atctcaacat cgtaaatagg ttcaagtatt ttcggacctg cattcttgaa tgccatgctg
    30961 aaggcatttc ttccggcaag cctgaaagaa atttcattgg aatcaacggg gtgcatttta
    31021 ccgtcataaa catataccct gatatccctc gcataggaac ctgtcagtgg ccctacttcc
    31081 attttttcca taataccttt caggattgca ggcatgaaac gcgcatcgat ggatcccccg
    31141 acaatgcagt taacatatat gagcctgcct ccccagtcga gatcaatttc ttccttggcc
    31201 ctgaccgaca gcttgatctc cttgttcccg aatttcatca tggtcagttc gggagaccct
    31261 tccgtatatg gttcaataat aaggtgaacc tctccgaatt gtcctgcacc ccctgactgc
    31321 tttttatgcc ggtattctgc ctgggctgcc ttggttatgg tttcacggta tggtatcctc
    31381 ggaggattga aatttgtcgg tatcttgtaa atattgtcaa gatgccattt cattatatta
    31441 agatggtatt cgccctgacc gtggaccagt atctgcttta gctccttgga gtattccaca
    31501 attatggtgg gatcttccag gtgtatcttg ccaagggctt cgccaagttt ctcatcgtca
    31561 ctttcactct gcgccttaat ggctgtgcgg tatttgggct ccgggtaact gatccccgca
    31621 tacttccagt cattgcccgg agcattcaaa gtatggtcgg ccttcgtgtt tttcagtttc
    31681 acaaaagccc ctatgtcccc cgtgttaagt tcctggacac gcacccggtt tttgccggca
    31741 caaacgtaca gttgagataa acgctccttt acaccattgt tgctgtttac aacttccatg
    31801 ttttcagtaa tttttcctga aaacaccctg aaatagttta tttcgccgat atgctcctca
    31861 atggatgact tgaaaacata tactgaagcc ggccctgcag gatcagcttt taccaaatct
    31921 cccctgctgt ttacgggagg cggcatgtcc gtgatggaag gtgcctcact gacgatgaat
    31981 tccatcaggc tgtcgacacc aatgttgttt tttgccgata aacagaaaac agggaatatt
    32041 ccccttgtaa caattccttt tgcaatacct ctccggattt cctcttccgt caggctgtca
    32101 tttgcaaaga actgctccat caacgattca tcgctttcag cagccttttc gacaagggca
    32161 ttatgcagtt caagtgcctt ctccatctga tcctcgggga tatcaagtat ttttgatatg
    32221 ccaccctcga ccggatactg aagcattttc atcctgagga catcaataat ggaattgaaa
    32281 ccggcacctt cattgacagg atactgaaca acatttgtat tgctcccgaa cctgtctttc
    32341 atcatttcta tcgacctctc aaagttggct ttttcatgat caagtccatt taccaccaga
    32401 atcagtggtt tgtgagcttt ctcagcatgc ctgaaagcta tttccgaacc tacctcaacc
    32461 ccgttctgta cgtttattgt aagcactgca caatcagtgg cgaagagaga tgaaacaaca
    32521 ttgccgctaa aatcatccag cccgggtgta tctataatat tgatcttttt gttcctgaat
    32581 tcactgtaaa ggacagtcga ataaattgaa ttgccgtttt catgttccac aggacgatag
    32641 tcggatacgg tattcctggc atcaacggtc cctcttcttt cgataacccc tccattaaaa
    32701 agcattgctt cacacaatgt tgtttttcct gatcctgaat tcccgagaat agctatgctt
    32761 tttatctggc cggtctggta tgttttcatg gttttttctg atattttatt ttttactgat
    32821 tgttttcaaa gcctgtttcg gaggggacaa aaataacaaa tttttaaaaa ctgtttaaga
    32881 cagagtgtaa attcaggatt ttttattttc tttgcaccag ttttttgaaa tgcaggagta
    32941 ccgggatttc tttcaaggta agtttataaa aagatttttc tcagggaaat gtcgggaagg
    33001 aacctgttga taacttacat ttttcagtaa gatattctgt tcaaagacgc aataaaggct
    33061 gattattaac agtcattgac acctgtttcg ggtttagctt gtgtttgaaa aaattatgac
    33121 aatagtttgt ggaataataa ataaaaacaa tacctttgca caccaatttt ttaagtagaa
    33181 tttaatgcta aaatcacagg atttatgccg acaatacaac agttagtaag gaaaggcaga
    33241 aaaaagctga tagataaaag taaggcacct gctttggatt cttgtcccca gagaagaggc
    33301 gtttgtgtac gtgtttacac caccactccg aaaaaaccaa actcggcaat gagaaaagta
    33361 gccagggtca agcttaccaa tcagaaggaa gtcaatgcat atattcccgg tgagggtcac
    33421 aatctgcagg aacactctat tgtcctgata aggggaggaa gggttaagga ccttccggga
    33481 gtgcgctatc atctcgtacg gggagccctc gatacatcag gggttgatgg tcgtactcag
    33541 cgaagatcga aatacggtgc aaaacgaccg aaaaaataac acttaaagga ttaagataat
    33601 gagaaaaaca aagccgaaaa agaggatcct tctgccggat ccgaaatata acgatcctta
    33661 tgtgacaaga tttgtgaata atcttatgta cagcggtaag aaaaaccttg cctttaagat
    33721 tttttatgat tcactcgaaa tagttggaga caagttcagg aacgaaggca aaacaccgct
    33781 ggaaatattc aagcaggcac ttgaaaatgt taccccgcag gtggaggtta aggccaggcg
    33841 tgtcggaggg gctaccttcc aggtgccaat ggagatccgc gccgacagaa agataagcat
    33901 cagcatgaaa aacatgattt cgttcgcaag aaaaaggaca ggtcattcaa tggctgaacg
    33961 tcttgctgct gaggttatgg ctgcttacaa actggaaggc ggcgcttata aaaagaaaga
    34021 agatacccac cggatggctg aagcaaacaa ggctttcgct catttcaggt tttaacccct
    34081 ttaaatccat ccacatttca gatacttaat ggattcaaac ctgaaatata ccagaaacat
    34141 cgggatcatg gcccacattg atgccggaaa gaccactacg acggagagga ttctgttcta
    34201 tacagaacgt actcatcgca tgggagaggt tcatgatggc aatgcacaga tggactggat
    34261 ggttcaggaa caggaaagag ggattaccat tacttcagct gccaccacaa ctttctggaa
    34321 atatcgcggg gataattata aaataaacat aattgataca cccggacatg tcgacttcac
    34381 tgttgaggtt gaaaggtcac tcagggtact cgatggtgcc gtggctattt tttgtgccgt
    34441 tggcggtgtg gaaccacagt ctgagaccgt atggcggcag gctaacaaat ataaggtccc
    34501 gagaattgct tttataaata aaatggacag ggccggagct gatttctttg gtgttgtcca
    34561 gcagatcagg gaaaagcttg gggcaaaccc agtgcctatt cagctgccga ttggctctga
    34621 agacgacttt aagggagttg ttgacctggt ttcaatgaaa acagtggttt acaatgacaa
    34681 tgatgccacc ggttctcatt ttactgttgg tgaaataccc gacgaattga ttgatgaagc
    34741 caatgaatgg aggggcagac tgattgaatc cgttgcagat gttgatgata cccttcttgc
    34801 acgatacctc gaggaccagt actcaataac cagtgatgaa atattggatg cactcagaaa
    34861 atcgtctgtc cacggcctgg cggtacctgt attatgcggt gcctctttca ggaacaaggg
    34921 tgttcagcgg cttcttgatg ccattgcggc attcctacct tcaccgcttg atatcggccc
    34981 ggttaccggt atagatccca gaaacgatag tgaagtgagc cgcgagccgg atgtgaacga
    35041 accccttgca gcacttgctt ttaaaattgc tacggatcct tttgtcggac gacttgtttt
    35101 ccttagggtc tactctggcg ttttaaaggc aggcgacacg gttctgaata cgcgcacggg
    35161 aaaacgggaa aggattaacc gcctcttcca gatgcatgcc aacaaacaga accccaagga
    35221 agtaatttat gctggcgata tctgcgccgg ggtcggcttt aaggatctta aaactggcga
    35281 tactttatgt gccatcaaca aaccgatact gctcgaatca atggattttc ccgaccctgt
    35341 gatcgggatt gcggttgaac ccaaaacaca ggctgatgtt gataaactgt cactggcact
    35401 taacaaactg gcagaagagg atcccacttt ccaggtaagg attgacccgg atagcggaca
    35461 gaccataata aacggtatgg gcgagcttca ccttgagatc ctcatagaca ggcttaaaag
    35521 ggaattcaag atcgaatgca accagggtgt accccaggtt gcctataagg aggcgatcac
    35581 aacgagtacc gaattccgtg aaattttcag gaaacaaacg ggcggcaagg gtaagtttgc
    35641 agacatagcg gttttaatga tgcccgggga aaatggtgta agaggactcc agtttgttaa
    35701 tgaagtcagg ggaggcaata ttcccaaaga attcattcca tcagttgaaa agggattccg
    35761 ggaggcaatg gctaacggcc cgctggcagg attccctctc gagagcctga agatagtact
    35821 gaaggatggt tcataccacc cggttgattc agattcactc tcctttgaaa ttgcagcgaa
    35881 acaggcgttc aggaaatatg ccggcaaatg caatccggta attcttgaac ccatcatgtc
    35941 gaccgaggtt gttacccccg aggaatatgt gggcgatgta ataggcgatt tcaacaggag
    36001 aaggggtaag gttgaaggta tggaatcaaa agccggttcg agggttatca gggcaaaggt
    36061 accactggcc gaaaagtttg gttatgttac cgtactgaga acacttactt ccggcagggc
    36121 aacttccaca atggagtttt cccattacga agaagtgcct gcagaaattg caaacagaat
    36181 agttgaaatt accaaaggaa aaattcttta aaaatgagtc agaaaatccg tattaaacta
    36241 aaatcttacg atcacaatct ggtggataag tcagcagaga aaattgttaa gacagttaaa
    36301 tccacggggg cagtcgtaag cggacccatc ccgcttccta cacacaagag aatatacact
    36361 gtccttcgct caccgtttgt aaacaagaag gcgcgggaac agttccagct ttcatcatac
    36421 aaaaggctgc ttgacattta cagttcaacc ccgaaaacga ttgatgctct catgaagctt
    36481 gaacttccca gcggggtgga agtagaaata aaagtgtgaa accggtaaag taatcataaa
    36541 atgcagggat taattggaaa aaaagtgggg atgacatccg ttttcgatga ggacggaaag
    36601 aatattccat gtacggtact gcaggctggt ccgtgtgtag tgactcaggt caaaaccagg
    36661 gagaaggacg gttattctgc cgtgcagctt ggttttgacg agaagaagga gaaacatact
    36721 acaaaagctg aattgggtca tttcaggaag gctggcacaa ctccaaggaa gaagctcgtc
    36781 gaattcacgg gttttgatga agggcaggtt aaggagggtg aggtgcttac tgccgaattt
    36841 tttacccata ctgactgggt cgatgtcagg ggctggtcaa agggaaaagg tttccagggt
    36901 gtggtcaaac gccatggttt cggaggtgta ggtgatcaga cccatggaca gcataacagg
    36961 ggcagggctc ccggttccct gggtgcttca tcttttccgt caagggttct gcctggaatg
    37021 aagatggccg gacggaccgg cggcaacaaa gtgatttctt atagcatgcg ggtagttaaa
    37081 ttaatgactg ataagaattt aattatagtc agaggatcag tccccggccc caaaggtggt
    37141 tacgtaataa ttacaaaata atggaattag cgattcttaa tattgatggt aaggaaaccg
    37201 gacgaacagt gaatctcaat gattctgttt tcggcattag accaaatgac catgccatat
    37261 atctggatac aaaacagttc cttgcaaatc agcggcaggg aacacataaa tccaagcagc
    37321 gtggtgagat tgccggcagt acaagaaaga tcaaaagaca aaaaggtaca ggtactgccc
    37381 gagcaggaag cgtgaaaaat ccgcttttcc ggggaggagg ccgtattttt ggtccggagc
    37441 ccaggttcta cggattcaaa ctgaataaga aagtaaaaca actggcaaga aaatcagcac
    37501 tttcctataa ggcagcaaca aataatatca tagttgtgga agacttctcc ttcgaaacac
    37561 caaaaacaaa ggaaattctg aaagttggag acagcctgaa tatcatgagg aaaaaatccc
    37621 tgattgtttt accggaacaa aataaaaaca tatatttgtc atcccgaaat gtacaagata
    37681 ttgaagttgt aactgctgaa gaattaaata cttacataat aatgagagct tcaactctgg
    37741 tactgttgga gagtgcggtt gacgttttgc aggctgcttt tgaaaatcag aaaactttga
    37801 gtgatgaata ttttgcttaa accgatagtg tcggaaaaga tgacaaacca gggcgacaaa
    37861 tacaatcgct atggttttta cgttgcaaaa aacgctaata aattgcagat aaagaaagct
    37921 attgaagagt tgtacggagt aaccgtcgac tcagtaaata cgatgcgata cggcggaaag
    37981 cgcaaaagca ggaacacccg ttcgggctac ctggttggaa aaactgccgc caccaagaaa
    38041 gctgttgtaa cacttgctga aggtaacaag atcgacttct acagcaacat ttaaaaacta
    38101 tcatggcagt aagaaaaatt aaacctgtaa caccaggtca gagaaacaag atcatcagcg
    38161 cttttgacaa aataaccagc gctgtgccgg agaaatccct cctccgcccg gttagaaaaa
    38221 ccggaggaag gaatgccagc ggcaggcaga ccatgcggta cattggtgga ggccataaaa
    38281 ggatgtacag gataatcgat tttctcaggc aaaaggatga catgcacgca actgtcagat
    38341 caatagaata tgatccaaac cgctcatccc gtattgcgct ggtcgtttat gaggacgggg
    38401 aaaagagata catcgtcgcg cctgccggcc ttgaggtagg acagaaaatt atctcaggta
    38461 aaaacgcttc ccctgaaatt ggtaatacaa tgttcctgaa tgatattccg cttggtacgg
    38521 tgattcataa tattgaactc aaacccggga aagggggcgc acttgccagg agtgcgggaa
    38581 cctatgcaca gctttcagcc agggaaggga aatatgtcac aatcaagctc ccttcagggg
    38641 aaacgagaat tgtactgaca agctgccggg caacaatcgg agctgtttcc aatcctgacc
    38701 acagtctcga aaaatcggga aaggccggac ggacacgctg gcagggacga cgcccgaggg
    38761 tcaggggtgt tgttatgaat ccggttgatc atccaatggg tggtggggaa ggccgcgctt
    38821 caggaggcca tccaagatcg cgcaagggtg ttcctgccaa agggtataaa acacgcgaca
    38881 aaaagaaata ctctgacaaa tatattattg aaagaaggaa aaaataattg atacattatg
    38941 agcagatc
//
LOCUS       DAOOYG010000013        38032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_910_length_38032_cov_6.951708, whole genome shotgun sequence.
ACCESSION   DAOOYG010000013 DAOOYG010000000
VERSION     DAOOYG010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38032
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_910_length_38032_cov_6.951708"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2658)
                     /locus_tag="PK288_02390"
     CDS             complement(<1..2658)
                     /locus_tag="PK288_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM17795.1"
                     /translation="MKINLNNSRSYSRFLPVPCSLALIMIMLAGPFSIVAGQKGGDQQ
                     KKISGTVRDENGQALPGAAILVEGTTSGTNTDAEGRFSIAVPGENSVLVFSFLGYTTQ
                     KVSAKGRNVIEVEMSPDTRTLDDVVVVGYGTQKKVNVSGAVDVVSSKALEKRAVANLS
                     QSLQGISPSLNISVGNSGGEMGARMNLNIRGIGSINGGSPYILVDGLEQDINNLNPDD
                     IESISVLKDAASSAIYGARAAFGVVLVTTKKGRNDGVSISYSNNFSFAAPTIVPHSVD
                     SKRFATYLNVASANDGNPPLFQNIILNFIDQYLAGEIDYWTVPFPLAPQYWLLNQGAW
                     ANTDWYDINYKKWVPNTLHNLSISGGDKRTQYYVSGSVFDQSGLLNFGKDDYGRYTLN
                     TKINTRILDWLRFNLASKFSSVNIERPSYDRNLLYDRLARQWPTNAPYFPNGTPNYEA
                     VQVWLEQGGMYKEKNNELSIIPGLEIEPLKGWIVYSNLRWRMNDWRMSNHEAKVKAWL
                     TDMTMTYLRPENNYSTNNFSGYYISPNLYSTYSGAFGEHSYVLMAGFEQELFTYESSY
                     AKRYDLISDEVPSLGTATGRQEVAGTKGHSATRSYFGRLNYSFNDKYLLELSIRYDGS
                     SKFPKGYRWGIFPSGSLAYLISRENFWKPFSEVVNQLKIRASFGSLGNQNVDNYLYVE
                     RLPVSTNLPYIMGNERPNYVGMAASISPGLTWEKVNTKNAGIDAGFLMNRLNLSFDYF
                     IRNTYDMLGPAESLPAVLGTAVPRSNNATLRTRGFELSLDWRDHYRDFSYGAKIVLSD
                     AVSTVTSYYNPEKLLSANYYEGMKLGEIWGFTTVGLFESDEQAQSVDQSYFSADILRA
                     GDVHYKDINNDGKIDIGRNTVADPGDKSII"
     gene            complement(2765..4846)
                     /locus_tag="PK288_02395"
     CDS             complement(2765..4846)
                     /locus_tag="PK288_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPM17796.1"
                     /translation="MKNSIVRVAVRKMLIPAIFFLLLSGCSRSPEIIWDKWGVPHIYA
                     GDAAGMYYSFGWAQMRSHHDLILELYARSRGRASEYLGSRYLESDKLMLTFRIPELAE
                     AGIEKMDPESRKYLDAFVKGMNDFIDLNRDSVDKELLQVYPVAPADILAHTYRVIWLE
                     FLGGEDVIKALRTMSPGSNAIALAPSKTTTGNAMLVINPHLPWHGFYTWFEAQLNYPG
                     FSCYGIALAGMPVLTLAFNNNLGWAHTVNPIDASDRYELTLKDGGYIFDGAVKQFTQK
                     NYTIRVKQPDGLFSEVAFEVKHSVHGPVTGISGNKALAVRIAGLEEFRIFEQYHKMAA
                     AGNFGEFESAIRMMQNPMFNIVYADRDGNIFYLFNGNIPVRKEGDFHFWNGTIDGTTS
                     GFLWDKYHSYEELPKLLNPETGFVQNCNEPPWFCTYPPVLKQSDYPAYFSSSWMTLRP
                     QRSINLIRSREKFSFEQLVDLKLNTGMEAADRFKKELVDASSASEDPLVREAGRILGD
                     WDGKTDTDSRGAVLFNAWWRLIEPGMFVRQWDPGDPVATPSGLKDPGKAAELLGKAAS
                     DVQNSYGSLSVPWGEVYRFRRGTIDYPANGGPGDLLGIFRTMSFERDSDNKFRAVHGD
                     TFHAVVEFGEEPHAMVLLAYGNSSQPGSRHYGDQIQYLSEKKMRPALLRKEDLRGNTE
                     LTETLKKSKKF"
     gene            complement(4851..6122)
                     /locus_tag="PK288_02400"
     CDS             complement(4851..6122)
                     /locus_tag="PK288_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17797.1"
                     /translation="MKKTEFSGKSLILIMIVGMITAAGCGTAGSNKKTDMTREYTKGT
                     YGYDKIFLEKNNISTIELTDPISGSRVLLAPGLQGRVMTSSADGDSGLSFGWINYKLI
                     ESGEQNTQFNPFGGEERFWLGPEGGPFSIYFRKGDEQVYSNWKVPDVIDTQPFEIVSA
                     GKDKTEFSQNFTVTNSTGTSLDVGVERTVKLLSGTEIMSALNVSLDGSVKYVGYESVN
                     RITNNGTEAWTEKSGFLSIWMLSMFTPSSSGIVFIPFKPGSTAAMGKIVSDDYFGTVP
                     PDRLVVRDDKIFFSIDGKFRSKIGISPERALPFAGSYDPDGKTLTILWFSLPGTPGPY
                     VNSKWGNQDNPLKGDAVNSYNDGPVEDGSIMGPFYEIESSSPAAFLKPGEKMTHVQRI
                     FHFTGSENELNRITEKIFSLKLEQITASLKK"
     gene            complement(6280..7293)
                     /locus_tag="PK288_02405"
     CDS             complement(6280..7293)
                     /locus_tag="PK288_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPM17798.1"
                     /translation="MEKKVSITDIARKVGVSVALVSYVMNGREKEKRVGKETVKKIRR
                     AAEELNYQPNQIARSLRLGSTKTIGLVVADIANPFFGQFARIVEDEAFKAGYTVVFGS
                     SDEDDIKSAFVLDTLLNRQVDGLIVIPSEGTVKQINGLVRKKKPFVLFDRYFKEVNCN
                     HIILDNYKGTNIATRYLVEKGHRKIALVAYKTSLVHMTERIRGYTEALKEAGIDHTKY
                     LAEIKFSHSYKDFENSFRAFFNKHSDIEALICANNGLSINSLYYIRQNCIRIPGDIDF
                     IGFDGGEPFDLYSTPLSFVRQPIEEMGREVVKLLTDLMNGSTKVSHIMLDPELVIREI
                     RNC"
     gene            complement(7606..8157)
                     /locus_tag="PK288_02410"
     CDS             complement(7606..8157)
                     /locus_tag="PK288_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPM17799.1"
                     /translation="MNKKNLFASITACLLIFFIHGCQSGTGGGSGLKVPGERSAAIDY
                     NMDMGGQPWAMNIEEATLGNGNYRAANWTGSNIQLVFMSLKPGEVIDLEVHDDHDQFF
                     RIEQGEARILMGKTRDDLSYDKRVSDDWSVLVPAGYWHKVENVGTNDLKLYTIYGPPE
                     HHKGTVHKTYNDAEEAHHHEHGQ"
     gene            8538..9368
                     /locus_tag="PK288_02415"
     CDS             8538..9368
                     /locus_tag="PK288_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPM17800.1"
                     /translation="MEEKLIYSGKSKNVYEITSGRYSGKYRLVFKDDATGYFENGKAV
                     FDPGYDTVVGQIPGKGAIACRFATHFFKLLQEHGIPSHYIDTVSDNEMIVEPALPLGI
                     PADSPEFPGSAPLQNLEFTWRNNATGSFWRRYPFVRPCRNIHKVVEAWTKGDTDILIT
                     FEALEETGAMTRAEIDYSVELVKKIAEIVTSEFGSKGLHVLDGKFELGRLKYGDRKIV
                     IIDEISPDVVRVCRGYAPDKDGNCTIYKDCTVTNFAEGKRTIKNRNQVKAADFISVFL
                     "
     gene            complement(9619..10416)
                     /locus_tag="PK288_02420"
     CDS             complement(9619..10416)
                     /locus_tag="PK288_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM17801.1"
                     /translation="MKKLLIVIFLISAGLLVYAQTDIKKLMPVTTGSSSALKVYTEAR
                     KYYEDVALDRAIETFKRALSQDPGFFMANYQLALYYLLNREADNFYKYARAAISCRDK
                     LSNAEEILKQALQNLMSGNSDVAYLGKKLIELYPADPESYNNLASFQSLEGDNDGMIV
                     TLSKALEIAPDPAPFYNQLGYAYLATKQNDKAEKAFDKYIELSPGNPNVYDSKGDYYM
                     YMKMYDKAYESYMKAHSIDPSFSRDKADTARRLYEMSEGRKIEIIQM"
     gene            complement(10558..11574)
                     /gene="mgrA"
                     /locus_tag="PK288_02425"
     CDS             complement(10558..11574)
                     /gene="mgrA"
                     /locus_tag="PK288_02425"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HPM17802.1"
                     /translation="MTENHQVSVNRYKNMKYNRCGKSGLLLPAISLGLWHNFGFADNF
                     NNSREIIRTAFDSGITYFDLANNYGPPPGSAEEVFGTILRKDFSAYRDEMVIATKAGY
                     LMWEGPYGEWGSRKYLIASLDQSLKRMGLDYVDIFYSHRFDPETPVEETMMALDHIVR
                     SGKALYAGISNYSPEQTEKAIRILKELGTPCLLHQSRYSMLDRKVESGLLDVIGSGGL
                     GFVAFSPLAQGVLSDRYLKGIPSDSRAAKAHFLKPSFITPELISVIGKLNELALQRGQ
                     TLAQMAIAWLLRDERVTSVIIGVSSASQLAGNIRALENISFTGNELEKIMALVTTVSF
                     NPRG"
     gene            11920..12408
                     /locus_tag="PK288_02430"
     CDS             11920..12408
                     /locus_tag="PK288_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17803.1"
                     /translation="METTSNPAEGSVSYEAYNKLQDLYIELLEKHKELTGEVPFQTAK
                     PKRKNLAVPFSKADWAATLNIGGEERKYDLSPVFRMIENEGLEYFEDTLRAVHENYAV
                     FYITELERVLNTPPETHDWFRGTLKNFQSELSLIKKLADAFRAVREVEPQSQAGTGSD
                     NP"
     gene            complement(12419..12829)
                     /locus_tag="PK288_02435"
     CDS             complement(12419..12829)
                     /locus_tag="PK288_02435"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase"
                     /protein_id="HPM17804.1"
                     /translation="MRNTDKLQGDLYTVQHTAQGKGEGAAAFRIAVNPDHEIFLGHFP
                     GNPVLPGACTIQILKELAEGASGRDLLLSGAQNIKFLSFIDPRKNPVLDIDLLLRETE
                     EGTYACSASVHYGGRVFCSFRGTMMLLPGNRTGA"
     gene            complement(12816..13355)
                     /locus_tag="PK288_02440"
     CDS             complement(12816..13355)
                     /locus_tag="PK288_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17805.1"
                     /translation="MNLVPWFGSERDHYLYNSGIDAFREHYSGLIVIKPLPDGDYRVV
                     FINELGIKIFDMEFFSTGEFLLHHCIAFLNRKFIINTLSKDISLALNNGQMKVIKVFR
                     GPGNDKSGFKARDGNGVKYILVNDADNRAEKIIRRGGLAKNAVVTFFNSGGTEPDSVN
                     ISHRHIKLDIHFSRIDEKH"
     gene            complement(13438..14082)
                     /locus_tag="PK288_02445"
     CDS             complement(13438..14082)
                     /locus_tag="PK288_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPM17806.1"
                     /translation="MNRFVKIISVLIMVLQTCLLAGQENMKPMNDPGSFSDRLIRASA
                     AVNSITSDFTQEKSLSVLNEKILSKGKFAFKKENNIRWEYTEPYRYLIIISNNQMFMR
                     DDKSRKQIDIQSNPMFREMNGFITGCIQGDILRNDNDYSKEYFENEKYYYVKLVPRSD
                     KVRQMLKEVHIWFNRSDLGVEKLKMVESGNDYTQIMFTGKILNPDIPVEKFTFK"
     gene            complement(14075..14848)
                     /locus_tag="PK288_02450"
     CDS             complement(14075..14848)
                     /locus_tag="PK288_02450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM17807.1"
                     /translation="MLKYKKICLLSLFLLMLLLLADQFIFISAWFYIGTVIATLALLA
                     YGSAAVCSGFYCRVICSGSTSERIISLTFDDGPHETLTPAILDILNKYDVKSAFFCVG
                     NRAAENPHLIMRMDKEGHIIGNHSGSHHFLFDLFSRKKIAEDLEENDKIIDRIINKKV
                     SLFRPPYGVTNPAVAAAVRARGYTVIGWSLKSRDTVIKNENLLLERLGQKLKNGGIIL
                     FHDNMDVTARILDRFIQHAAERNFRFERLDHLLKINAYE"
     gene            complement(14842..15906)
                     /locus_tag="PK288_02455"
     CDS             complement(14842..15906)
                     /locus_tag="PK288_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HPM17808.1"
                     /translation="MKIFINGQSAISPQETFTGNSLPDNPVNYSANGFIKCIEPSVRE
                     YIDPMTARRMSRIIRFGVCSAIQCLRNADAGNPGAIITGTGLGCLDDTEKFLRTMHNN
                     EEKLLNPTPFIQSTHNTLSSAIAIIIGCNSYNSTYVHRGGSFENALTDAILLLRENSA
                     ENVLVGGVDELTDTSFNITSRLGFWKREPVESLRLLDYKGRGSLAGEGMTFFLLSNVK
                     NERSFAVTEMPELFHKPADAAASEEKIRSFISRTCQEGSIDLVLMGLNGDISNDEIYH
                     NLSGRLFSGIPRAYYKHLCGEYDTSSAFAMFIAAEIIRNQTVPRVMQLDGGPAGKIRN
                     VLIYNHQRNNIHSLMLLSGC"
     gene            complement(15908..17116)
                     /locus_tag="PK288_02460"
     CDS             complement(15908..17116)
                     /locus_tag="PK288_02460"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPM17809.1"
                     /translation="MAVRVFVTGIGIISSIGFNCEETEDSLKSGKTGIGTMKNLVSVH
                     SGEIPVAEVPASNEELEDLARIRKTGYSSRTTLLGIIAAGEALRCSGLDEDIGSFRTG
                     LISATTVGGMDRSEKFFKSYIQHKSGGRLRYIVTHDCGDSTAAIADTFGIRDHVSTIS
                     TACSSSANAILTGARLIKGGLLDRVIAGGTDALSLFTLNGFNSLMILDKKGCRPFDED
                     RNGLTLGEGSAFIVMESETVTARIGKKLLCEVSGYGNACDAYHQTASSPEGGGAYLSM
                     KQALHCSGLTPDMIDYINAHGTGTKNNDLSEGRAIERLFGYLPVKFSSTKAFTGHTLG
                     AAGAVEAVLGIMAIRNGWIYPNPGFARQMSELRITPEKKLIRNTRIRNLMSNSFGFGG
                     NNTSLIFSEC"
     gene            complement(17186..17449)
                     /locus_tag="PK288_02465"
     CDS             complement(17186..17449)
                     /locus_tag="PK288_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPM17810.1"
                     /translation="MEKLVQTLKEEIIEALNLVEMVPEDINEEAPLFVDGLGLDSIDA
                     LELTVLLEKKYGIKIKTAEEGKKILYSVKSMADCIIAGGKAWD"
     gene            complement(17453..18091)
                     /locus_tag="PK288_02470"
     CDS             complement(17453..18091)
                     /locus_tag="PK288_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17811.1"
                     /translation="MTGYYITGYCIIRNNEASVNGKPVFSAHGKNTREFFTDLYKFLD
                     SDYPKFHKMDNLCKLGFLSSELILKKNGVRTIFSGSDTGVILANSSSSLDTDRMHQVT
                     IINRAAYFPSPAVFVYTLPNIVIGEICIRNKITGEGSFFIQEKLNPVFLTDYIRCLLD
                     CEIINRCIAGWLQVDGDDCDAMMCLIEKTDKPISGFVNFEPSVIRDIYNLRI"
     gene            complement(18088..19227)
                     /locus_tag="PK288_02475"
     CDS             complement(18088..19227)
                     /locus_tag="PK288_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPM17812.1"
                     /translation="MTDVFIVSDNIITSLGFSTGENASNMTAGITGIRLISDESLSPD
                     PFYVSLVDTDRLRGTFSQILDPEEFTRFEQMAILSIKAAVAATKIDFSGERTLFILST
                     TKGNIDLLGKNTGKNDPGLYLWHTAKRLGDFFRFRINPLVISNACTSGLQAVITGSRL
                     IRAGYYDNVVINGTDIISEFVVSGFSSFKSLSTGPCRPFDSTRDGLSLGEGSGTIILT
                     NNKGASKQTFRIRVGMGFSGNDANHISGPSRTGEGLFLVTDRVMKNSPSMPDFISAHG
                     TGTLYNDEMEAIALTRAGLQNIPVNSFKGYWGHTLGAAGVIELAMCVHSMNNNVLFPT
                     KGFREPGLENKLNVIDRVIKTEVNACLKTASGFGGCNSALYLFKT"
     gene            complement(19270..19695)
                     /locus_tag="PK288_02480"
     CDS             complement(19270..19695)
                     /locus_tag="PK288_02480"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPM17813.1"
                     /translation="MKKKMLIAETLCNVRFSDVDSMGIVWHGNYVRYFEDGREDFGRK
                     YGLTYLDFYHKGIFIPIVKVTCDYKKPLLYGDIARITTKFVNCESAKISYEYIIYNNK
                     TNEIAATGSSLQVFLDINRELLLSVPAFFIDWKRENGLF"
     gene            19863..19991
                     /locus_tag="PK288_02485"
     CDS             19863..19991
                     /locus_tag="PK288_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17814.1"
                     /translation="MKQRKNAKRNPEVPVPSERDLHDLQAAQNLKGNKDQIDYSQW"
     gene            complement(20024..20389)
                     /locus_tag="PK288_02490"
     CDS             complement(20024..20389)
                     /locus_tag="PK288_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HPM17815.1"
                     /translation="MRKLLLPLPAVILLTSVELLANKTSVEIKAPSASVKGTEVTVTI
                     NVTHKGNSKMHHTDWVWLKVNGQEVKRWEFSKEHLPAAGDFTLEYKITVTDDLKLEAE
                     GHCNIHGTAGAKSMVIKAE"
     gene            complement(20487..21080)
                     /locus_tag="PK288_02495"
     CDS             complement(20487..21080)
                     /locus_tag="PK288_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17816.1"
                     /translation="MKAKRVIATVLFVMLALGHAWGQDRAKALSLTDCVWFGIDFTGA
                     RFTSVTEDPAVIVGQYLNAINTLILQEPAKFDIKKFFKKTGVVNSIETATERNSKIDP
                     GKLVISQEYQITTDDVSRIISSYRNDDNSGTGLIFVAENLNKITQTGSYYVCFFDIAS
                     HEIIDCRKITGKAAGFGFRNYWAGSVYNAMKNWAAAK"
     gene            complement(21363..22655)
                     /locus_tag="PK288_02500"
     CDS             complement(21363..22655)
                     /locus_tag="PK288_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM17817.1"
                     /translation="MFIPEIEKKPKREVDAFQSGKLAQLLRYLEKNSVFYRDHFRQNK
                     IDPEKIRNINDLVTIPPTRKSDLQERARDFICVRSEKIIDYLTTSGTSGEPVIYVMTE
                     NDLNRLAYNEYLGFSCADCTRNEVFQLMTTIDRRFMAGMAYFLGSRKLKSAMVRVGNG
                     IPELQWDSIKRFSPTALIAVPSFLINIIDYAERNGIDHHAASVKKAVCIGDSLRNPDF
                     TFNTLGRKITERWPEIKLYSTYASTEMAASFTECSYGMGGHHHPELLVVEFLDENDSP
                     VAEGEEGELTITTLGVEGMPLLRFKTGDICQGHYEPCRCGRTSMRVGPVIGRKNQMIK
                     YKGTTLYPPALYDILENIDGVINYVVELYTNTIGTDEILIRIGTNISSESFEKAIKDH
                     FRAKLRVAPSITFESAEYVEKIQFPGMSRKPVKLIDRR"
     gene            complement(22636..24372)
                     /locus_tag="PK288_02505"
     CDS             complement(22636..24372)
                     /locus_tag="PK288_02505"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HPM17818.1"
                     /translation="MSWTRYEMHKNRKIKKGILKGALLMLFLLLTVFIINFYRATVEH
                     PPKAGDLSSLQLVRIETDTNKFFYGNNWLRKSKSGLYEMYLEGGPFERGVAFGKLTRE
                     LLYYQEEAFVEQIRKLVPSETYLKFLKYFVGWFSRDLGKHIPEEYLLEIYGTSFSSSP
                     AFDFIGTGYQRQLNYHAAHDIGHALQALKMTGCTSFSFWGGKSSDSTLITARNFDFYA
                     GQKFAENKIIVFCNPESGLKFMMITWADMIGVVSGMNEKGLTVTINAARSAIPLKATT
                     PVTILAREILQYAGDIAQAVSISEKRKLFVSESLLISSAADGKTVIIEKSPEKFDVVL
                     PQSDQIICANHFLGSAFSDDKTNIENIYGSDSKYRFERVGELLDMVDKVSVRDAAAIL
                     RDRKGRGGTDPGMGNPLAINQLIAHHSVIFSPSDLLVWISAGPYQLGEYVAYDLKKVF
                     SGETAGDPVSREISEPDLSIPADTFLFSADYSCYGKYLKMTGDLDNYTRNHEKLPESF
                     ETAYIETNPMLYLVYSNLGNYFYENRLYEKANEYYQMALSKDVAGEDERNKLLKLTEK
                     VHKKLGNVHSRN"
     gene            complement(24344..25834)
                     /locus_tag="PK288_02510"
     CDS             complement(24344..25834)
                     /locus_tag="PK288_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPM17819.1"
                     /translation="MKYDVIIVGSGLGGLQCAYIMSREGFKVCLIEKNKQLGGCLQTF
                     KRKGSVFDTGMHYIGSVEDGQLLHKFLKYFRLCGKLKIRKMDTNAYEIIRLGDREFSF
                     ANGYDHFSEKLLSYFPGESEAVRNYVAKMQEVSRSVDLLNMDTLPGKRPGYFNYFSTG
                     FNDYLDSLTGNRELKSLLTGNSPLYAGVRDRTPLYTPMIINSTYVDSAYRFVDGGSQV
                     SDLLSGGIVENGGTIMRNARVTKFLFSSGLMKSVVINDSEELSAKWFISNIHPKAMLS
                     LMENAPVRPAYQKRIASIDDTYGMFSLYLAMKENSFEYINSHYYIYRTDDVWHGDRIA
                     PGRWPESYMMHFSPGSKNEKYTGSVIVTAYMNWNEVSPWENTWIEQRGDDYIDFKTGR
                     AEKLLEVVANDFPGIRGKIKSYYTSTPLTYRDYTGTFRGSVYGLQKDFNSPLKTLIIP
                     RTKVPNLFLTGQNTNVHGVVGVTIGSVLTCGEMLGTQYVLDKIRNA"
     gene            26055..27197
                     /locus_tag="PK288_02515"
     CDS             26055..27197
                     /locus_tag="PK288_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPM17820.1"
                     /translation="MFREEDFEDIRPYHDDEINPALRRIISNPVFDTILDFFFPYDEK
                     DKIRKLLSETRSVYSFQKNFVDRLVRAIVSKSSSGLTCSGFENLTPGKAYLFISNHRD
                     IVLDSAILQILLFEHGHNTSEITFGSNLMVNQFVIDLGKSNRMFRVVRGGSSRELLHN
                     SQQLSAYIRYTIVSKGISVWIAQRPGRTKNGNDRTETSLLKMLNMSGRGKPEDSLHEL
                     NIVPCTVSYEYEPCCALKIKELIATSLTGTYQKQPGEDLNSIIKGIMQQKGRIHFAIG
                     SPLDKSLGQLSNESTFNRKINTLAAIIDKEMYRNFRLWPNNYISWDIQNHSGRFREFY
                     TPDDKAKFLEYMENETGGLTGDRNMINDLFLQIYANPVSNYLTLNR"
     gene            complement(27210..27815)
                     /locus_tag="PK288_02520"
     CDS             complement(27210..27815)
                     /locus_tag="PK288_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17821.1"
                     /translation="MKKLLSVILIQVLIAGSISHAQKFTLKKGNLGFLKGEKSLLVTF
                     DYSNMAVGKFDREDDYIAKKVEDYNKSEAGKGEKWKEAWKGDRASRYEPKFEQLFNRD
                     AEKTGLTGTRDVTDARFEMNIHTTFTEPGFNIGITRKPAYIDLVITFRNISSGDETAV
                     IEVRNCPGRDALGYDFDTGYRIEEAYAKLGKSLAAFIAKQR"
     gene            27927..30452
                     /locus_tag="PK288_02525"
     CDS             27927..30452
                     /locus_tag="PK288_02525"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPM17822.1"
                     /translation="MYLKAGKKLFLSIYLLFHSFVFTTEAQTIVRGRVTDLQTFEPVV
                     FANIVFKGTTIGTQSGFDGSYVLSGITGSDSVVISFVGYTTKTIGIIPGSDQDIDIRL
                     SPAIFSIGEVTVRPGLNPAIKLLREVWKNIPENHIDKLQSYQYVNYSRSTLYIRKPGY
                     YKKTGRNLEKPHSERFEKYSVKTGEEDIPAIPSYFTEILSENFHLKSTGQNFIHIIGS
                     NSEGIAFETTSLPAQLITKQENFNFMDNTVLIIDRSFISPLSRSGLFYYKYYLRDTML
                     IDNRYQCFRVDFIPKREEDPVFRGSFWINDTTFALKRISVEVGEKAELNFIRRVKIQQ
                     DYEPAGGGAWFPVSTRFMADAANLFLTNFSRKTEIIVNNPKQPGFYSSELKVSFDVNN
                     RDPGFWEKSRMNSLEKTDSLALQKIGILKENSGISIAAKLIEASIRGYYDFGKFEAGP
                     YLLFYGRNDTEGSRFRIGGRTNSRFSNRLTLEGYTAFGTRDKRMKGSVQAAYLLSEKY
                     WTSVGLQLRDDLEYAGAIDEFYSQNSFLTFASTFGGSDMMARTTIIRPWIGSDITRGL
                     QAKMVLTRKTFEPAGKEYHFAWYAGKDGTGLKDNFMTNELGLILSYQPGAVYVLDGNR
                     RFAVNFNQYPVFSLEYFRGFKNPGKGDFSYDRISAGIDHRFNPGGLGTVDWNARYSKV
                     FGALPYPLLTTFAGNESIFRADRTCNLMNLGEFVADESLELFLSYHMNGLLLGKLPLI
                     KKLEWRTVFSGRVAYGSFNEKSNGFYDPESNPAGILSKLDPAGYPVSEFSTLSWRHPY
                     AEISYGIENIFRFFRIDLIQRLTWLDNPGSRRLAVKISGVFRF"
     gene            complement(30472..34329)
                     /locus_tag="PK288_02530"
     CDS             complement(30472..34329)
                     /locus_tag="PK288_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM17823.1"
                     /translation="MENFFLFLFGYFKSRRILFFTIVTAILLIAALLASRISFEEDIA
                     KMISGVDRKTEITTIFNRSKFLDKIIVNISLADTSAPPAPDRLILVGKKLYDTLQTGD
                     FPQYLSDVTFRIPDNIMEDVMAIVYRNLPLFLEEEDYVALDSLIAPEKIRESLENSYN
                     SLLTPASFAMKRNILMDPAGLTSIVTRKLRAFQNDINYLIIDGSIFSRDKKSLLMFLT
                     TTYSSNVTSKTSRLIKELDGVINAISREAGSQIRIEYFGSAAVAAGNADRLKRDISLT
                     ITITIILLFLIITFSIKKKALIPFIFLPAVFGGLTALAVIYLVQAKISIIALSIGTVI
                     LAITVDYALHITAHYKHKHSVAETIRDVSFPIIVCGFTTAFEFMSLVLVSSESLHELG
                     ILAAISVITAAFFTMIVMPQILDVTRREDDETEEKNYLERILDKVTNYEFDKNKRLLL
                     FMVIATVVSWFYFYKVGFESDMMKMNYMSKKLAIAEENLNRINNYKLNSVYVIAHGED
                     LQEALVNNEKLMAEGKTMMAENRIRTFNSPGALLSSDSLQKKRIERWNLFWNRNKKDS
                     FLKNFRYYSARTGFRQDAFEEFYNLLDRKYTLMDPGDFRKLADHFYSENITYAPGLTA
                     IVSVMKTDARNKDYVEEEIGKIDGVVVIDRKSILSEVVRVLGRDFDFIANVSLSIILV
                     IMIIVFGRFELGFITFIPILVSWFWTLGFMGITGIKFNIFNIIISQFITGLGIDYSIY
                     IMQGLVQGYKTGTRNLLSYKTCILLSVLISISGTGVLILAKHPALKSIALISIVGLLS
                     VVLISYTFEIVVFNWLVRKKGKNRYLPMTISEVLLTVVTLTAGLVLCVLLNILLIAVL
                     LVPVSGKKKRKFMHHALMWCIRLPVYGLTGIRKTILNRENADFSRPSLIISNHQSHLD
                     LPLLLMLNPKIIILTNNWVWNNPLYALVIRYLHFYPVSWGYENLIEKLEKEIGEGYSI
                     LVFPEGTRSPDSEILRFHKGAFLMAEKLGLDITPILIHGAGDCMRKGENHLRKGSVTL
                     KIYPRVAATDTDFGHDYHQRTRTFLRFYREEYKVLRKELETPSYFRNRLVSNYIYKGP
                     VLEWYTRIKLRLERNYEYINSLVPENAQVVDIGCGYGIISYMLGFTSENRNILGIDYD
                     ENKIELANNCISKNDRISFVAADAVTYDYPDADVFLLSDVLHYVPRERQAGLLASCIR
                     RLKPGGKIIIRDADSDLQKRHLGTRFTEFFSTTFGYNKSLDRRLFFFSGRFISELASG
                     YNMQLEVIDNSRLTSNKLFVLSR"
     gene            complement(34330..35496)
                     /locus_tag="PK288_02535"
     CDS             complement(34330..35496)
                     /locus_tag="PK288_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HPM17824.1"
                     /translation="MRYCVIIPTYNNDSTLEKVINGVREFTENIIVVNDGSTDRTAQI
                     LSNVILVNGSLKVIAYQVNRGKGYAIRQGLDLAIKEGYDYVITIDSDGQHDPADIPSF
                     IAAVENSPDSLITGDRNLGVKNISRGSRFANKFSNFWFRFLTGIKLNDTQTGFRLYPL
                     GPLKSMRFFTVKYEFETEILIRAAWKGIKISSVPVRVYYPPAEERVSHYRFFRDFARI
                     SLLNTCLVLAAVFYIIPFSSLRNLNKEKIKEFVNKHILLTRETNLKISLAIAFGIFMG
                     IVPIWGFQLVTALALAHIFRLSKFLVAVAANISIPPMIPLILYLSYATGGIVLGTGSR
                     ISFSGEITLKTFENNLLQYITGAFIFAAVFAAFAGLVSFVVLKLVRRKPVILSM"
     gene            35847..37394
                     /locus_tag="PK288_02540"
     CDS             35847..37394
                     /locus_tag="PK288_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPM17825.1"
                     /translation="MVVIGEHFLTTEDFKKLLYGGEMIEISSEAKGRVSRSFDFLRSF
                     HRDKIIYGINTGLGPMAQYRIEDEDRINLQYNAIRSHASGCGQPVDPVYVRSSLIVLL
                     NNFLQGHSGIHPEVVELISRFINNDISPVVPEHGGVGASGDLVQLAHIALALIGEGDV
                     NFGGRVIPAAEAMRKSGLEPIKVHIREGLSLINGTCFMTGTGMVNIIHARNLVSWALI
                     ASAMINEIVHSCDDSFSKELNEVKKHPGQRDVARVIREILADSNLIRKRENEFYNNNH
                     NHTKKILNDKVQEYYSIRCVPQIAGPVLDTIRESEKVLLNEANSSCDNPMIDDENLNI
                     YHGGNFHGDYVSFEMDKVKIAVAKLSLLIDRQINYLMNDKLNNILPPFVNLGKLGVNF
                     GMQGAQFTSTSTVAENQSVSFPNYLHSIPNNNDNQDIVSMGTNSALLARRVIDNTYQV
                     LAIQMLSLVQAIDYLRIEDNLSSRTRQAYRQIRDIVPVFVEDTVKYPEIRLIKDFLFS
                     RLLSFFD"
     gene            37411..>38032
                     /locus_tag="PK288_02545"
     CDS             37411..>38032
                     /locus_tag="PK288_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM17826.1"
                     /translation="MKYALVTGGSRGIGRAVCTGLVPLGYHVIINYRSNDAEAEKTLA
                     MINQAGGTGELMKFDVSDSRQVDGALNRWNEQHGDDYIEVLVNNAGIRKDMLMMWMEN
                     DSWREVLSTNLDGFFYVTRALLKNMLVRKYGRIINIVSLSGIKGMPGQVNYSAAKAAI
                     IGATKALAQEIGKKNVTVNAVAPGFIRTDMTSELNENELKGLIPLNR"
BASE COUNT         9997 a         8975 c         8302 g        10758 t
ORIGIN      
        1 tattattgat ttgtcgcccg gatctgcaac cgtgtttctt cctatatcga ttttcccgtc
       61 gttgttgata tccttataat gaacgtcgcc tgctctcaat atatccgcag agaaatatga
      121 ctggtcgacc gactgagcct gttcatcaga ctcaaagagg ccgacagtgg taaatcccca
      181 gatctcgccc agtttcattc cttcataata attggcactg aggagttttt cgggattgta
      241 atagcttgtg acagttgaga cggcatctga aaggacgatt tttgcaccat atgaaaaatc
      301 acgataatga tcccgccagt cgagggaaag ttcaaatccc cttgtcctga gggttgcatt
      361 gtttgacctt ggaacagccg tacccagaac agccggtaat gattcagccg gccccagcat
      421 atcgtaggta ttacggataa aataatcaaa gctcagattg agcctgttca tgagaaaacc
      481 tgcatcaata cctgcgttct ttgtatttac tttctcccat gtaagcccgg ggcttattga
      541 agcagccatc cctacgtaat tgggtctctc attacccata atatacggca ggttggttga
      601 aacaggcagt ctttcaacat aaagataatt gtcgacattc tgattcccca gggaaccgaa
      661 tgaagccctt atcttaagct ggtttaccac ttcggaaaaa ggtttccaga aattttccct
      721 cgagatcagg taagccaggg atcctgatgg gaaaattccc caccggtaac cttttggaaa
      781 ttttgaagac ccgtcatacc ttattgataa ctccagcaga tacttatcat tgaaactgta
      841 atttaatcgt ccaaaatagc ttctggttgc tgaatgaccc tttgttcccg ccacttcctg
      901 ccttcccgtg gcagtaccaa gggaaggcac ctcatcactt atgagatcat atctttttgc
      961 atacgacgac tcataagtga aaagctcctg ttcaaaccct gccatcaaaa catagctatg
     1021 ctctccaaaa gctcctgaat aggtactgta aagatttgga gatatatagt acccgctgaa
     1081 gttgttagtt gaataattat tctccggacg caggtaagtc atagtcatat ctgtgagcca
     1141 tgccttcact tttgcttcgt gatttgacat cctccagtcg ttcattctcc atcgaaggtt
     1201 tgaataaaca atccagccct taagtggctc aatttcgagc cctggtatta tactcagctc
     1261 attgttcttt tccttgtaca ttccaccctg ttcaagccat acctgaaccg cctcgtaatt
     1321 aggggtcccg ttagggaaat acggggcatt ggttggccat tgtcttgcta gacggtcgta
     1381 caggagattc cggtcataac tgggtcgttc aatattaacg ctgctgaact tgcttgcaag
     1441 attgaatcgc agccagtcga ggatcctggt attgattttc gtgttcaggg tatacctgcc
     1501 gtaatcgtct tttccgaaat taagcaggcc cgactggtca aaaaccgaac ccgaaacata
     1561 atactgggtt ctcttatccc cgccgctgat gctgagatta tgaagagtat tgggaaccca
     1621 ttttttataa ttgatgtcgt accagtcggt atttgcccag gcgccctggt tcaggagcca
     1681 gtattgcggt gcaagcggaa agggtactgt ccagtaatct atctcacctg caagatactg
     1741 atcaatgaag ttgagaatta tgttctgaaa aagtggaggg ttcccgtcat tcgcggaagc
     1801 cacgttcaga taggtcgcaa atctctttga atcgactgaa tggggtacta ttgtcggtgc
     1861 tgcaaaggaa aaattgtttg aataactgat tgagaccccg tcatttctgc ctttctttgt
     1921 cgtgacaagg acaactccga aagctgccct ggcaccgtaa atagcagatg aagcagcatc
     1981 cttaagaacc gagatgctct caatatcatc cggattaaga ttgtttatat cctgctccag
     2041 cccgtcgaca agtatatagg gggaacctcc gtttattgaa cctatccccc tgatgttaag
     2101 attcatacgg gcacccatct caccccccga attccctacg ctaatgttga ggcttggaga
     2161 gatgccctgg agcgactgcg acaggttggc cactgccctt ttctcaaggg cttttgaact
     2221 aaccacatca accgcacccg atacattgac ctttttctgt gtcccgtatc ccactaccac
     2281 aacatcatca agggtccggg tatcagggct catttcgact tcaatcacat ttctgccttt
     2341 tgcggatact ttttgggttg tgtatccaag aaatgaaaaa accagtaccg agttttcgcc
     2401 aggtaccgca attgagaatc tgccttccgc atcggtattt gtaccggaag tggttccttc
     2461 aaccagaatc gcagcgccgg ggagagcctg accgttctca tcccttaccg tgccgctgat
     2521 ctttttctgc tgatctcctc ctttctgtcc tgccactata ctgaaaggac cggccagcat
     2581 gatcattatc agtgccaggc tgcagggaac aggcagaaaa cggctgtatg aacggctatt
     2641 gttaagattg attttcatgt gaaaataaaa ttatatgcta ctgaagatta agtggttcac
     2701 atggaacccg catgaatttt attttcatgc ttgtttgtga ttttgtatca tgcgggtttc
     2761 ataatcagaa ctttttgctc tttttaaggg tttcagtcag ttcagtattt ccgcggagat
     2821 cttctttcct tagcagtgca ggccgcatct tcttttccga caggtattga atctgatcgc
     2881 cataatgcct gctgccgggc tgcgaagagt taccataagc caataacacc atggcatgag
     2941 gttcctctcc gaattcgacc actgcatgga aagtatcacc atgaactgcc ctgaatttat
     3001 tatccgaatc cctttcaaaa ctcattgtcc tgaagatacc cagcaggtcg ccgggcccgc
     3061 cgtttgcagg ataatcaatg gtaccgcgcc tgaaacgata tacttcgccc cagggaacac
     3121 tcagggaacc atatgaattt tgcacatcgg atgcggcttt cccgaggagt tccgcagcct
     3181 ttcccggatc cttcagtccc gacggggttg caacaggatc gccaggatcc cactgcctga
     3241 cgaacatgcc cggttcaata agtctccacc aggcattgaa caacacagca ccgcgactgt
     3301 cggtgtcagt ttttccgtcc cagtctccca gaatcctgcc agcttcccgg accagggggt
     3361 cttccgaggc cgatgaagca tcaaccagct ccttcttaaa cctgtcggca gcctccatcc
     3421 cggtattcaa cttaagatca accagttgtt caaaggaaaa tttctcccgg ctccttataa
     3481 ggtttattga acgctgaggc ctgagcgtca tccatgagga tgaaaaatag gcaggataat
     3541 ctgactgttt cagcacaggg ggatatgtgc agaaccaggg cggctcgttg cagttctgaa
     3601 caaatccggt ttccggattc agcagctttg gaagttcttc ataactatgg tatttgtccc
     3661 ataaaaaccc cgacgttgta ccgtcaatgg tcccgttcca gaaatggaaa tcgccttcct
     3721 tcctcaccgg gatattacca ttgaaaagat aaaagatatt cccgtccctg tcagcataaa
     3781 caatgttgaa catggggttc tgcatcatgc gtatggccga ctcgaactca ccgaagttcc
     3841 ctgcggcggc cattttgtga tactgttcaa atatcctgaa ttcctccagt ccggcaatcc
     3901 tgacggccaa tgccttgttt ccgctgatcc cggtaaccgg tccgtgcact gaatgcttaa
     3961 cttcaaaagc gacttcggaa aataatccgt ctggttgctt tacgcggatg gtgtaatttt
     4021 tttgtgtgaa ttgtttcaca gctccgtcaa atatatatcc tccgtccttc agtgtaagtt
     4081 catagcggtc ggacgcatcg atgggattga ccgtatgggc ccatcccagg ttgttgttga
     4141 aagccagagt caaaaccggc attcctgcaa gggcaatacc atagcagctg aaaccagggt
     4201 aattcagctg tgcttcaaac caggtataaa agccgtgcca tggaagatgg ggatttatca
     4261 caagcattgc attacccgtt gtggtttttg aaggggcaag tgcaattgca ttcgatcccg
     4321 ggctcatcgt gcgcaaggct ttaattacgt cttccccgcc aagaaattca agccagataa
     4381 ccctgtaagt atgagccagg atatctgccg gggcaacagg ataaacctgc aaaagctcct
     4441 tatctaccga gtcgcggttg agatcaatga aatcattcat tccttttaca aatgcatcca
     4501 gatatttccg ggactccgga tccatttttt ctattccggc ttcggcgagc tcagggatcc
     4561 tgaaagtcag catcaatttg tcgctttcca ggtacctgct ccccaaatac tcggaagcgc
     4621 gacccctcga gcgggcgtaa agctcaagta taagatcgtg gtgtgatctc atctgcgccc
     4681 atccgaatga ataatacatc ccggcagcat cgccggcata tatatgcggc actccccatt
     4741 tgtcccagat aatttcaggg ctgcggctgc aaccggaaag aagaaggaag aaaatggcgg
     4801 gaatcagcat tttccgcact gctacgcgga ctatggagtt tttcattgat ttattttttt
     4861 agtgaggcgg tgatctgctc aagtttgagg ctgaaaatct tttctgttat cctgttcagc
     4921 tcattttcgc tgccggtaaa atggaagatg cgctgcacat gcgtcatttt ttccccgggt
     4981 ttaagaaatg ctgctggaga agagctctca atttcataaa agggacccat tatggagcca
     5041 tcttccacag gcccgtcatt ataggaatta accgcatcgc ccttgagcgg attgtcctga
     5101 tttccccatt ttgagttgac atatggaccg ggagtcccgg gaagggaaaa ccataaaatt
     5161 gtaagagttt tgccgtcggg atcgtagctg ccggcaaaag gcagtgccct ttcgggcgag
     5221 atgccgattt tgcttctgaa tttgccatcg atgctgaaaa agatcttgtc atcccttacc
     5281 acgagcctgt cgggaggaac agtaccgaaa taatcgtcgc tgacaatttt gcccatggcg
     5341 gccgtgctgc ccggtttaaa cggaataaag acaatgccag atgatgaggg cgtgaacatc
     5401 gacaacatcc atattgaaag gaagccggac ttttcggtcc aggcttcggt gccattgttc
     5461 gtgatcctat taaccgactc atatcccaca tatttgacgg aaccgtcgag actgacattg
     5521 agtgccgaca taatttccgt ccctgacaaa agtttgacag tcctttcaac gccgacgtcc
     5581 agggaggttc ccgtactgtt tgtaacagtg aagttctggg aaaattcggt tttgtcctta
     5641 ccggctgaga caatttcaaa tggctgcgta tcgattacat ccggcacttt ccagtttgaa
     5701 tatacctgct catcaccttt ccggaaatag attgagaacg gaccgccttc cgggcccagc
     5761 cagaaccttt cctcaccgcc aaaggggttg aactgggtgt tctgttcacc gctttcaatc
     5821 aatttatagt ttatccagcc aaaacttaag cccgaatctc catctgctga agaggtcatc
     5881 acccttccct gcagtccggg agcaaggaga accctgcttc cgctgatggg atcagtcaat
     5941 tcaattgtgg atatgttatt cttctccagg aagattttgt catatccata ggttcctttt
     6001 gtgtattcgc gtgtcatatc ggttttttta ttactacctg ctgttccgca acctgccgct
     6061 gtgatcattc ccacgatcat gatcaggatg aggctttttc cgctgaattc agtttttttc
     6121 atagaagttg ttttaatccg tttaagtgag cccgggattc cgcctgcatg aaaacacggg
     6181 ttcgttgatc aatgaaatta taatcaattt atattgcagt catcgcgtga tttataatgt
     6241 cagtatgttg actaaacgtt taagcataat ttccagggat cagcagtttc tgatttccct
     6301 gataaccagt tctggatcaa gcatgatatg ggacactttg gttgagccgt tcatgagatc
     6361 cgtcagaagt ttcacaactt cgcgtcccat ttcctcgatg ggctgcctca caaatgaaag
     6421 tggcgtggaa taaaggtcga atggttctcc tccgtcaaag ccgataaaat caatatcccc
     6481 gggtattctg atgcaattct gtctgatgta ataaaggctg ttgatcgaca agccgttatt
     6541 ggcacagata agtgcctcga tatcgctgtg tttgttaaag aatgccctga aactgttctc
     6601 aaaatccttg tacgaatgtg agaattttat ctcagccagg tattttgtat ggtcaattcc
     6661 ggcttctttc agggcttcgg tgtagcccct tattctttct gtcatgtgaa ccagtgatgt
     6721 tttgtaggca acaagcgcta ttttcctgtg gcccttttca acaaggtaac gtgttgcaat
     6781 gttggttccc ttataattat cgagaatgat atgattgcag tttacttcct taaaatacct
     6841 gtcgaacagc acaaagggtt ttttctttct aaccagtccg tttatctgct tgacagttcc
     6901 ttccgagggt atcactatca atccgtcgac ctgccggtta agcagggtgt caaggacaaa
     6961 agccgatttg atatcatctt catccgagct tccaaacaca actgtatacc cggccttgaa
     7021 tgcttcatct tcgacaatcc ttgcgaactg gccgaagaat ggattggcaa tatctgccac
     7081 aacgagtccg attgtttttg ttgaacccag cctgaggctc ctggcgatct gatttggctg
     7141 ataattgagc tcttcagctg ccctgcggat cttttttacc gtctctttcc caacccgctt
     7201 ttccttttcc cggccgttca ttacatatga aaccagggct acggaaactc ccactttcct
     7261 ggcaatatcg gttattgaaa ccttcttctc catatgaaaa caaaattcta cacaaatata
     7321 tgcttaaacg tttaaatata aaaatagttt ttgcttattt caaataaaaa attgcctgtt
     7381 cccttattcc tttcatctgc cggccctgat cctgttccaa accattcgga accgtcatcc
     7441 agataaggga gtggaaaaac gcgtggtata aagaaagact ggcctggcag taaatgctca
     7501 cgaatgatgc aacaactact ataattatcg ccgggtaata tcacgatccc cccgaaggat
     7561 tcaacgggta atcaaaaacc atgaccgatt cgcaacagtt gtttcttact gtccgtgttc
     7621 gtgatgatga gcctcttctg catcattgta ggttttgtga accgttcctt tatgatgctc
     7681 cggaggtcca tatatggtgt aaagctttaa gtcattggtc ccgacattct caaccttgtg
     7741 ccagtagcct gcaggaacca gcacggacca atcgtcggac actcttttat cataactcag
     7801 gtcatccctg gtttttccca tcaggatccg ggcttcgccc tgttctatac gaaaaaactg
     7861 atcatgatca tcgtgtactt ccagatcaat cacttcaccc ggcttcaggg acataaatac
     7921 aagctgtata ttgctgccgg tccagttggc cgccctgtaa ttgccatttc cgagagtagc
     7981 ttcttcaata ttcattgccc agggctgtcc gcccatatcc atattgtaat caattgcagc
     8041 agatcgttca cccgggactt taagtcccga acctccgccc gttccggact gacagccatg
     8101 aatgaaaaaa atcagtaagc aggcagtgat tgaagcaaat aagttctttt tattcataat
     8161 gattgatttt tgatttattg caagttatga aattccgccg cctggcgggc tataaactga
     8221 tatttggcac aagatcggag gtgttttttt gcgcatctat tcccggtatt tctcagggga
     8281 atgctgccgg acattggtta aggcagaaat cgtaccataa aaagtcgcag ggggggaatg
     8341 acccagatta tgattatccg gcctgacatg aaatacccgg ttttgtacgg gtgaaaaaat
     8401 aatttaccca aaattattcc tgattgttct ccctgcatta tctttaccgg tctcgttcaa
     8461 gaacatatac agggcgagaa tgacgattta ataaaataca attgaaacat ccttaaattc
     8521 ttataagatt cagggcaatg gaagaaaaac ttatttacag cggtaaatcc aaaaacgtat
     8581 atgagatcac ttcgggccgt tattcgggta aatacaggct cgttttcaag gatgatgcca
     8641 ccggctattt tgaaaatggc aaggctgttt ttgatccggg ttatgatacc gtggttggcc
     8701 agatcccggg aaaaggagcc attgcatgca gattcgccac ccatttcttc aagctgcttc
     8761 aggaacatgg gatcccgtca cactatattg acacagtcag cgacaatgag atgatagtgg
     8821 aaccagcact ccctctgggg atacctgcgg actcgcctga atttcccggc tcggctcctc
     8881 tgcagaacct tgagtttacc tggagaaaca atgctaccgg aagtttctgg aggagatatc
     8941 cctttgtaag gccctgcagg aatattcaca aggtggtgga agcctggaca aaaggtgata
     9001 ctgatattct catcaccttt gaagcattgg aggaaacagg agcaatgacc agggctgaaa
     9061 ttgactacag cgttgagctt gtgaaaaaaa tagcggaaat tgtaaccagc gaatttggtt
     9121 ccaaaggact gcatgtcctt gacggtaagt tcgagctggg gcgtctgaaa tacggggaca
     9181 ggaaaattgt gatcatagac gagatatcgc ccgatgttgt acgggtatgc aggggatatg
     9241 cccccgacaa ggacggtaat tgtaccatat acaaggattg caccgtaaca aatttcgcag
     9301 agggcaaacg gaccataaaa aacaggaacc aggttaaggc agccgatttt atatctgttt
     9361 tcctgtaatc aggtgcttca tccggaactg atccgcccgc ttggctgaga tcagctttta
     9421 ccggagatta cgccgggaag cctttaaaac tgccgggtaa taccggtatt accaacaacg
     9481 cggaaatact aaaagtctgg caacggactt gtttgttatc attgtcagca aaacccccgg
     9541 ttcatggtca ttattttgat gccaggcacg ggttttttat ggcttcagtg tcttttcacc
     9601 tttgaaattc cggcagaatt acatctgtat tatttcgatt ttcctgcctt ctgacatttc
     9661 atagagcctc ctggccgtat ctgccttgtc acggctgaag gaaggatcga tggaatgagc
     9721 tttcatatac gattcataag ctttgtcgta cattttcata tacatgtaat agtcgccctt
     9781 tgaatcgtat acgtttggat taccgggaga aagttcaata tacttatcga aagccttttc
     9841 cgccttgtcg ttttgtttgg ttgccagata cgcgtatccc agctgattgt agaacggggc
     9901 cggatcgggc gcaatttcca gggcttttga caatgtaact atcataccgt cattgtcgcc
     9961 ctcaagcgac tgaaaggaag caagattatt gtatgattcc ggatcagcgg gatataactc
    10021 aatcagcttc tttccaagat aggccacatc tgaattgccg gacatcaggt tttgcagcgc
    10081 ctgtttcagt atctcctcgg cattgctcag cttgtccctg caagaaattg cagccctggc
    10141 atatttgtag aaattatcag cttcacggtt tagcagataa taaagagcca gctgataatt
    10201 ggccatgaaa aaaccgggat cctgggagag ggcccttttg aaggtctcaa tggccctgtc
    10261 gagtgcaaca tcttcatagt atttcctggc ttctgtgtaa accttcaaag cagagcttga
    10321 acctgttgtc acgggcataa gcttttttat gtcagtctgg gcataaacca gaagtcctgc
    10381 tgaaatcagg aatatgacaa ttaatagctt tttcattttt tttacataat ggttcctata
    10441 aacaattgaa cccagggttc agttcacttt tcccggggtc gtataacatc agcaaatatc
    10501 atgcaattca gacccggtag aacagggaat ttgtcataat ttccaaagca ttgcttatca
    10561 gccgcgcgga ttgaagctga cagttgttac aagtgccatg atcttttcca gttcattacc
    10621 ggtaaaactt atattttcga gtgccctgat gttcccggca agctggcttg cagaactgac
    10681 cccgattata accgaggtta cccgttcatc ccgcagcagc caggcgattg ccatttgtgc
    10741 aagtgtctgg cccctttgca aagctaattc attcagcttt cctataacgg aaattagctc
    10801 gggagtgatg aatgaaggtt tcaggaagtg ggcttttgca gcgcgggagt ctgacggaat
    10861 acctttcaga tacctgtcag ataatacacc ttgtgccagg ggagagaatg ccacaaaccc
    10921 gagtccgcct gatcctataa catccaggag gccgctttca accttcctgt cgagcattga
    10981 atacctcgac tggtgcaaca ggcagggggt accaagctct ttcaggatcc ggattgcctt
    11041 ctctgtttgt tccggggaat aatttgagat accggcatac aatgccttac cgctcctgac
    11101 tatgtgatcg agagccatca ttgtctcttc aacaggggtt tcaggatcaa acctgtggga
    11161 gtagaatata tcgacataat caaggcccat tctcttaaga ctctgatcaa ggctggcaat
    11221 gagatacttt cttgaacccc attcgccata aggtccctcc cacataagat aaccggcctt
    11281 ggtggctatc accatttcgt ccctgtatgc agaaaaatcc ttccttagta tcgttccaaa
    11341 gacctcttcg gccgacccgg gaggaggtcc gtaattgttt gccagatcga aataagttat
    11401 cccgctgtcg aaggcggtcc tgattatctc tcttgaatta ttgaaattat ctgcaaagcc
    11461 gaaattgtgc cacaggccaa gggaaattgc aggtaaaagc aggccgcttt ttccgcatcg
    11521 gttatatttc atgttcttat acctgttgac tgatacctgg tgattctctg tcatacttaa
    11581 ctttttgaga tggatcctgg atggtccaaa tttatgaaaa ttcccaatcc tttctccatc
    11641 ccggatatga tcggggcgac tggcgctggt gacgggcatc accggtaggg atcccgtccg
    11701 aaaaacaaaa aacgcaattc tgccctcaat tcgttacaaa tttctgtttg aactgaagca
    11761 tcgtattcaa ataagtttat ttatatttac cataactaac ctgacatcag tgtgtgtgtg
    11821 tgttttagta ttatgttaaa gcctgaattt taaccttgct tttccatagc tttgtaaata
    11881 ttaattattt caattccagg aggattttta tctatagaca tggaaacaac cagtaaccct
    11941 gctgaaggat ccgtttcgta tgaggcgtac aacaagcttc aggaccttta tatcgaactg
    12001 cttgaaaaac acaaggaact caccggggaa gtgccgttcc aaactgcaaa accaaaaagg
    12061 aagaacctgg cggttccgtt ttcaaaggca gactgggcgg cgacccttaa tattgggggt
    12121 gaagaacgga aatacgatct gagtccggta tttagaatga ttgaaaatga gggtctggaa
    12181 tattttgaag ataccctccg ggctgtacat gagaattatg cggtgtttta tattaccgag
    12241 ctggagcgtg tgctgaacac ccctcccgag actcacgact ggttcagggg gacactgaaa
    12301 aatttccagt ctgaactttc actgatcaag aagctggctg atgccttcag ggcagtcagg
    12361 gaagttgaac cgcaatcaca ggccggcaca ggatccgaca atccctgagc atcaaggatc
    12421 aggccccggt acggttcccc ggcaacagca tcattgttcc ccggaagctg cagaacaccc
    12481 tgcccccgta atgaaccgat gcactgcagg catatgtccc ctcttccgtt tccctcaaca
    12541 ggagatcaat gtcaagtacc ggatttttcc ggggatcaat aaacgaaagg aatttaatgt
    12601 tctgcgcccc ggaaagcagc aaatccctgc ccgatgctcc ttcggcaagt tcctttaata
    12661 tctggatcgt gcaggccccg ggcagaaccg ggttgccggg gaaatgcccc aggaaaatct
    12721 catgatccgg attaacggct atcctgaagg ctgcagcccc ttcccctttc ccctgcgctg
    12781 tgtgctgaac tgtataaagg tcaccctgca atttatcagt gtttctcatc tatcctggaa
    12841 aaatgaatgt cgagttttat atgcctgtga cttatattta ccgaatcagg ttcggttccg
    12901 cctgaattga aaaaagttac aactgcattt tttgccaatc cgccacgtct gattatcttt
    12961 tctgccctgt tgtcagcatc attgacaaga atatacttaa caccgtttcc gtcccttgcc
    13021 ttgaagcccg atttatcgtt gcccggtccg cggaacacct taataacctt catctgcccg
    13081 ttattaagtg caagactgat atccttactg agtgtattga tgatgaactt cctgttcagg
    13141 aatgcaatgc aatgatggag caggaactcc ccggtgctga aaaattccat atcaaatatc
    13201 ttgataccaa gctcattgat aaacaccacc cggtaatccc cgtcaggaag cggctttatt
    13261 acaattaatc cggaatagtg ttccctgaag gcgtctatcc ccgaattata caggtaatga
    13321 tccctttcac tgccgaacca tggtaccaga ttcatctcac ggaaattatc cgtaccggtc
    13381 cggcggaatt ccctgaaaac ggacggagca cagcctgaaa atattataag gcaaatacta
    13441 cttaaaagta aatttctcaa cgggtatgtc tggattgagt attttcccgg taaacattat
    13501 ctgtgtataa tcattgcccg attcaaccat tttcagcttt tccacgccca gatcgctcct
    13561 gttgaaccag atatgcactt ccttcagcat ctgcctcacc ttgtccgacc tgggcacaag
    13621 cttaacatag taatactttt cattctcaaa gtattccttt gagtaatcat tgtcattcct
    13681 gagaatgtcg ccctgaatac aaccggtgat gaatccgttc atctccctga acatcgggtt
    13741 tgactggata tctatttgct tcctgctttt atcatcacgc atgaacatct ggttgttgct
    13801 tatgattatg agatacctgt agggttcggt atattcccat cggatgttgt tttccttttt
    13861 gaatgcaaac tttccctttg aaagtatttt ttcattaagg acactcaggc ttttttcctg
    13921 ggtaaaatca cttgttatgc tgttaacagc tgctgatgcc ctgataagcc ggtctgaaaa
    13981 ggagcccgga tcattcatgg gtttcatgtt ttcctggcct gccagtaagc acgtctgcaa
    14041 caccattatc aatactgaaa ttattttgac aaatctattc ataggcattg atttttaaca
    14101 gatgatcgag cctctcaaac ctgaagttcc gctctgcagc atgctgaatg aacctgtcaa
    14161 gtattcgtgc tgtaacatcc atattgtcat gaaagagaat tattccgcca ttcttcagtt
    14221 tctgtccgag tcgttcaagc agaaggttct cattttttat cacggtatcc cttgatttca
    14281 ggctccatcc aataacggtg tatcctcttg cccgaacggc agcggccacg gctgggtttg
    14341 tgaccccata cggaggtctg aacaaactga cttttttatt aatgatccgg tcgatgatct
    14401 tatcgttttc ttcaagatcc tctgcaattt ttttcctgct gaacaaatca aagaggaaat
    14461 gatgcgaacc ggaatgattt ccaataatat gtccttcctt gtccatcctc attattaaat
    14521 gcgggttctc agctgccctg ttccctacac agaaaaatgc actcttcacg tcgtacttat
    14581 tcaatatatc gagtatagcc ggtgtcagcg tttcatgggg tccgtcatca aaagtaagcg
    14641 agataatcct ttcacttgtg ctgcccgaac aaataaccct acagtaaaaa cccgagcata
    14701 ctgctgctga accgtaagcc agcagggcca gtgttgcaat aactgtacca atataaaacc
    14761 atgcacttat gaatataaac tggtcagcaa gcaggagcag catgagcaga aaaagggaaa
    14821 ggagacatat ttttttgtac ttcaacatcc tgaaagcagc atgagggaat gaatgttgtt
    14881 tctctgatgg ttgtagatca gaacgttccg tatttttccg gcaggtccgc cgtcaagttg
    14941 cattacccgg ggcacagtct ggttcctgat tatttcagcc gcaataaaca ttgcaaaagc
    15001 tgatgaagta tcatattcgc cgcacagatg cttgtagtat gcccggggga tcccggaaaa
    15061 aagtcttcct gagaggttat ggtagatttc atcattagat atatccccgt tcaatcccat
    15121 taagaccagg tcgatactgc cctcctggca tgtccggctg ataaatgatc gtattttttc
    15181 ctcagaagcg gcagcatcag ctggcttatg aaacaattca ggcatttccg ttacggcaaa
    15241 tgatctttca tttttcacgt tgctgagcag aaagaatgtc atcccttcac ctgcaagtga
    15301 tcctctgcct ttataatcaa ggagtctgag gctttcaacc ggttcccttt tccagaatcc
    15361 gagcctgctg gttatattga aagaggtgtc tgtcagttca tcaaccccac ctacaagaac
    15421 gttttcagca gaattttccc gcagcaggag aattgcgtca gtcagagcat tttcaaatga
    15481 accgccgcgg tgcacatatg tgctgttgta gctgttgcac ccgatgatga ttgcaatggc
    15541 ggatgagaga gtattatggg ttgactggat aaacggagtg gggttaagga gtttctcttc
    15601 attattgtgc attgtcctca ggaacttttc cgtatcatcc aggcagccaa ggccggtacc
    15661 ggtaatgatc gcgccgggat tgccggcgtc agcgttcctt agacactgaa tggcagaaca
    15721 tactccgaac cttatgatac ggctcatgcg ccttgccgtc atgggatcga tgtactccct
    15781 tacagatggc tcgatacatt tgataaaccc gttggcagaa taattcaccg gattgtccgg
    15841 aaggctgtta ccggtgaatg tttcctgggg agatattgca ctctgcccgt tgatgaatat
    15901 cttcatgtca gcattctgaa aagatcaggc ttgtgttgtt accgccaaat ccaaatgaat
    15961 ttgacataag gttcctgatc ctggtatttc gtatcagctt tttttcaggt gttatcctta
    16021 gttcgctcat ctgccgtgca aacccgggat ttggatagat ccacccgttc ctgatcgcca
    16081 ttatgcccag aacggcttcg acagctccgg cagcaccgag ggtatgcccg gtgaatgcct
    16141 ttgtggagct gaacttcacc ggcaaatacc caaaaagtct ttcaatagcc cgcccttccg
    16201 acaggtcgtt gttttttgtt cctgtgccgt gagcattgat ataatcaatc atgtcagggg
    16261 taagcccgga gcagtggaga gcctgcttca tcgacaggta tgcacccccg ccttcgggag
    16321 aggaggcggt ctggtgatat gcgtcacatg catttccgta acccgatact tcgcataaaa
    16381 gctttttacc gatcctggcc gtaacggttt ccgactccat tacaatgaat gctgatccct
    16441 cacccagtgt aagtccgttc ctgtcctcat caaacggacg gcagcctttt ttatcaagta
    16501 tcatcaggct gttgaagcca ttaagggtaa aaagtgaaag ggcatccgtc cccccggcaa
    16561 tcaccctgtc gagcaatccg ccttttatca gcctggcacc cgtcagtatg gcgttggcgg
    16621 aggatgagca tgccgtacta atggtcgaga catgatccct gatgccgaag gtatcagcta
    16681 ttgctgcggt gctgtcgccg cagtcatgag tcactatgta tctcagccgc ccacccgatt
    16741 tgtgctgaat atagcttttg aaaaacttct ctgaacgatc cattcctccc acggtcgtag
    16801 cagatatcag gcccgttctg aaactgccga tatcttcatc caggccgctg catctcaatg
    16861 cttcccctgc cgcaatgatg cccagcaggg tggttctcga actgtatccc gttttcctga
    16921 tacgggcaag atcctcaagc tcctcattgg atgcaggcac ctcagcgaca ggtatctccc
    16981 cagaatgcac cgaaaccagg tttttcattg tgccgatccc cgtctttccg cttttcagcg
    17041 aatcctctgt ttcttcacaa ttgaaaccaa tggatgaaat gatcccgata ccggttacaa
    17101 atactctgac tgccacactc aatcaatttg atttccactc tgccgggagc ccctggtcat
    17161 gggcactccg ggaataaagc aggtactaat cccatgcctt cccgcctgca attatacaat
    17221 cggccattga cttaaccgaa taaaggattt ttttgccttc ttcggcagtc tttattttta
    17281 tgccatattt tttttccagc aaaacggtca gttcgagcgc atcaatggaa tccaggccca
    17341 atccgtcaac aaacagcggc gcctcctcat tgatatcttc cggaaccatt tcaaccaggt
    17401 taagggcttc gatgatctct tccttgaggg tctgaacaag cttctccata cgtcaaatcc
    17461 ttaaattata aatatcccgg attactgaag gttcaaaatt aacaaatcct gaaataggtt
    17521 tatctgtttt ttcaattaaa cacatcattg cgtcacaatc atcaccgtcg acctgcagcc
    17581 agcccgcaat acatctgtta attatttcac aatcaagcag acacctgata taatctgtca
    17641 ggaaaacagg attgagcttt tcctgtatga aaaagctgcc ttcaccggtg atcttgtttc
    17701 ttatacagat ctcaccaata acaatatttg gaagggtata aacaaaaacc gccgggctgg
    17761 ggaaataggc tgcacggtta attatggtaa cctgatgcat tctgtctgtg tcgagtgagg
    17821 aggaggaatt ggctagtatt accccggtat cgcttccgct gaaaatggtc cggaccccgt
    17881 ttttcttaag aatcagctcg gaagacagga atccgagttt gcacaggtta tccattttat
    17941 ggaatttcgg ataatcggaa tccaggaatt tgtaaagatc tgtaaaaaat tccctggtgt
    18001 ttttcccatg ggcgctgaac acaggttttc cgttaacact ggcctcattg tttctgataa
    18061 tgcaataacc cgttatataa taccctgtca tgtcttgaat aaatacagtg cagaattgca
    18121 gcccccgaag ccggatgcgg ttttgagaca ggcgttcact tccgttttga tcacccggtc
    18181 aatgacattc agtttatttt ccaatccggg ttcacggaat ccttttgttg gaaaaaggac
    18241 attattattc attgaatgga cacacattgc cagttcaatc acgccggcgg ctccgagggt
    18301 gtgtccccaa tatcccttga aactgttcac agggatattc tgcagtccag ccctggtcag
    18361 tgcaattgcc tccatttcat cgttatataa ggtccctgtt ccgtgggcag aaatgaaatc
    18421 cggcattgaa ggtgaattct tcatgactct gtctgttacc aggaacaatc cctcgcctgt
    18481 ccttgaagga cctgaaatat gatttgcatc attgcccgaa aatcccatac cgacccttat
    18541 ccggaatgtc tgctttgaag cgcccttatt gttggtgagg attatggttc cggagccctc
    18601 acccaatgag agtccgtcgc gggtactgtc aaaaggccgg catggccctg tactgagaga
    18661 tttaaacgag ctgaagccgg atacaacaaa ttctgaaata atatccgtcc cgtttattac
    18721 tacattgtca taataaccgg ccctgataag gcgcgatccg gtaatgacag cctgcaatcc
    18781 tgatgtacag gcgtttgaga tcaccagcgg attaatcctg aatctgaaaa aatcgccgag
    18841 tcgttttgcc gtatgccaga gatacaatcc gggatcattt ttgccggtat ttttcccgag
    18901 cagatctata ttgcccttgg ttgttgaaag gataaaaagt gttctttccc cgctgaagtc
    18961 aattttggtt gcagcgacgg cggctttaat tgacaggatg gccatctgct caaaacgagt
    19021 gaactcctcc ggatcaagta tctgagaaaa tgtacccctt aaccggtcag tatctactag
    19081 agaaacataa aaaggatcag gggagaggct ctcatcactg attaacctga taccggtgat
    19141 cccggccgtc atgttagacg cattctcacc ggtggaaaat cctagggatg taattatatt
    19201 gtctgataca ataaaaacgt cagtcactaa cccgaatata aaattcgctg caaatttaaa
    19261 aattctgttt cagaaaagac cgttctccct tttccagtca ataaagaatg caggaacgct
    19321 cagcagcagc tccctgttaa tgtcgagaaa aacctgcagg cttgatccgg tggctgctat
    19381 ttcattggtt ttattgttat aaataatata ctcataactg atcttagccg attcacaatt
    19441 cacgaatttt gtggttatcc ttgcaatatc gccgtacagc aggggttttt tataatcaca
    19501 cgtcaccttc acaatgggaa tgaatattcc cttatggtaa aaatcgagat aagtaagtcc
    19561 gtattttcgt ccgaaatctt cccgtccatc ttcaaaatac ctgacataat ttccatgcca
    19621 gacgatcccc attgaatcaa catcgctgaa ccttacatta caaagggttt ccgcgatcag
    19681 catttttttc ttcatgaagt cccattgatt tgttattgta cattttccct gccgtgaata
    19741 ataaggatct cctgatctga atcggaggca ggtctgccgg ccattcagtt tcaatcgcag
    19801 gttaatcaga atattccctg cgaagaatct tcaattcata aatagattaa agcaatcgat
    19861 gcatgaagca gcgcaaaaac gccaagcgca atcccgaggt tccggtgcca tctgaaaggg
    19921 atcttcacga tcttcaggcc gcccagaatc tgaagggcaa caaggatcag attgattatt
    19981 cccaatggta aaaccagaaa atacataccg gcaatttttg taattattca gcctttatca
    20041 ccatgctttt tgctccggcg gtcccgtgaa tattgcagtg tccctctgct tcaagcttaa
    20101 ggtcatctgt aacagttatt ttatactcca gtgtgaaatc accggcagcc ggcagatgtt
    20161 ccttactgaa ttcccagcgc ttcacttcct gcccgttaac ctttagccaa acccagtcgg
    20221 tgtgatgcat tttgctgttt cccttatgtg tcacatttat agtaacagta acctccgtcc
    20281 ccttcacgga tgccgaagga gcttttattt ccacactggt tttgtttgcc agcagttcaa
    20341 ctgacgttaa caggataacc gccggaagag gtaacaacaa ctttctcatt ttatgaatgt
    20401 ttttaattta acattattac aaagcaacca tgcaaagaac aaatgtattt cccggtcagt
    20461 ccaggccggt aaacccttat ttcttactat tttgccgccg cccaattctt catggcgttg
    20521 tacacggatc ctgcccagta gttcctgaaa ccaaaaccgg cggccttacc cgttatcttc
    20581 cggcaatcga ttatctcatg cgaggcaatg tcaaagaaac aaacataata gctgccggtc
    20641 tgggtgatct tattcaggtt ctcggcaaca aagatcaatc ctgttcctga attgtcgtca
    20701 tttctgtaac tgcttataat acgactgacg tcatcggttg tgatttgata ctcctggctg
    20761 ataaccagtt tcccgggatc aatttttgaa ttccgttcgg tagctgtttc aatactattt
    20821 acgactccgg tctttttgaa gaatttctta atatcaaatt tcgcgggttc ctgaaggatc
    20881 agtgtattta ttgcattcag atactgaccg acaattacgg ccggatcttc agtaacagaa
    20941 gtaaatctgg caccggtaaa atcaataccg aaccatacac agtcagtaag gctcaaagct
    21001 ttagccctgt cctgtcccca agcatgaccc agggccagca taacgaaaag tactgtcgct
    21061 ataactcgtt ttgctttcat ggtaaaaggt tttaattctg atatctttct tacttttccg
    21121 aattaacggg aaaaaccgta attgcattca aactgattcc attttgcggt taacctgctt
    21181 cattcgcctg gcaggctgaa tttcataccc tattggtatt tcaacatacc aagttacaaa
    21241 attccatgtt tcatggacat ttcacttgta aaaatcatgt tacaggtctt atcctcaatc
    21301 tggcccgggc gcctgtttac ctttcgtccc tgatatcagc ctggtatctg cttacgtcag
    21361 atctatcttc tgtcgatcag tttcaccggt ttacggctca tgcccgggaa ctgtatcttt
    21421 tcgacgtact ctgcagattc aaatgttatt gaaggggcca ctctcagttt tgccctgaaa
    21481 tgatccttta tggccttttc aaagctttcc gaagaaatat ttgtaccgat ccggatgaga
    21541 atttcatcgg tcccaatggt gtttgtgtac agttcaacaa catagttgat caccccgtca
    21601 atattctcca ggatatcata aagtgcaggc gggtagaggg ttgtaccctt atatttgatc
    21661 atctggtttt ttcggcctat taccggtccg accctcatcg acgttctgcc gcacctgcaa
    21721 ggctcatagt gaccctggca gatatctccc gttttgaatc taagcaaagg catgccttcc
    21781 actcccaggg tggtgattgt cagctctccc tcttcacctt ccgccaccgg tgaatcgttc
    21841 tcatcgagaa actcaactac aagcagctcg ggatgatgat gtccccccat tccgtagctg
    21901 cattcggtga atgatgctgc catttccgtg gaagcatatg tggagtaaag ctttatttcg
    21961 ggccatcttt ccgttatttt acggccaagg gtgttgaatg tgaaatcggg atttctcagc
    22021 gaatcaccga tacatactgc ctttttaaca ctggcagcat gatgatcgat accgttcctt
    22081 tccgcataat caataatgtt gataaggaaa gacggtacgg caatcagggc agttggcgag
    22141 aaccttttta tgctgtccca ctgtaattcg gggatcccgt ttcccaccct taccatggct
    22201 gatttcaatt ttctcgaacc caggaaatat gccatgcctg ccataaaccg gcggtcaata
    22261 gtggtcatga gctggaacac ttcattcctt gtacagtcag cacaagagaa accaagatac
    22321 tcgttgtacg ccagccgatt caggtcattt tccgtcatga cataaatgac cggttccccc
    22381 gatgtccctg aagtagtcag ataatctatg atcttttcac ttctgacgca aataaagtca
    22441 cgggctcttt cctgcagatc tgatttcctg gtggggggaa ttgttaccag gtcatttatg
    22501 ttcctgatct tttccgggtc gatcttgttt tgcctgaaat gatccctgta gaacactgaa
    22561 ttcttctcga ggtaccggag caattgagcc agctttccgg actggaaggc atccacctcc
    22621 ctttttggct tcttttcaat ttcgggaatg aacatttcca agctttttat gaactttctc
    22681 agttagtttt aacagtttgt ttctttcatc ttctcctgct acatcctttg acaatgccat
    22741 ctgataatac tcgttggcct tttcatataa gcggttctca taaaagtagt ttccaagatt
    22801 ggaatataca agataaagca tgggattggt ttcgatatat gcagtttcaa aagattccgg
    22861 cagtttttca tggtttcgtg tataattatc aagatcaccg gtcattttaa gatatttccc
    22921 gtaacatgaa tagtcggctg agaacagaaa agtatcagcg ggaattgaca gatcaggttc
    22981 gctgatttcc ctgctgacgg ggtcccctgc agtttctcct gaaaatacct ttttcagatc
    23041 atatgccaca tattcaccca gctggtaagg accggcggat atccatacca gcagatcgga
    23101 tgggctgaaa atcactgaat gatgggcaat aagctgattt atagccagtg gatttcccat
    23161 ccccggatca gttcctcccc tgccctttct gtccctgagt attgctgccg catcgcgcac
    23221 actaaccttg tcaaccatgt caagcagttc ccccaccctt tcaaacctgt atttgctgtc
    23281 tgatccatag atattctcaa tgttggtttt atcatctgaa aaagcactgc ccaggaaatg
    23341 attagcacaa atgatctgat cactctgagg aaggactacg tcgaattttt ccggcgattt
    23401 ttcaatgatc accgtttttc cgtcagctgc agagctgatc aatagcgact ctgatacaaa
    23461 cagtttccgt ttctccgaga tactgactgc ctgggcaata tccccggcat actgtaatat
    23521 ctcccgcgca aggatggtaa ccggggttgt tgccttcagc ggaatagccg accttgcggc
    23581 attgatggtc accgtaaggc ctttttcatt catccccgaa acgactccga tcatatccgc
    23641 ccatgtaatc atcataaatt tcaggccgct ctcaggattg cagaaaacaa ttattttatt
    23701 ctcggcaaat ttctgtccgg catagaaatc aaagttgcgc gccgttatga gggttgaatc
    23761 cgaacttttc ccgccccaga atgaaaagga agtgcagccg gtcattttca aagcctgcaa
    23821 tgcatggcca atgtcgtggg ctgcatgata gttcagctgc ctctggtaac cggtaccaat
    23881 gaagtcaaat gccggagagc tggaaaagga agtaccgtaa atctcaagaa gatattcttc
    23941 ggggatatgc tttccaagat cccttgaaaa ccatcccacg aaatatttca ggaatttaag
    24001 atatgtttct gaagggacca gttttcgtat ctgctctaca aatgcctctt cctggtaata
    24061 aaggagttcg cgggtcagtt ttccgaaagc taccccgcgt tcaaatgggc cgccttcaag
    24121 atacatttca tacaggcccg atttactctt tctcaaccag ttattgccat aaaagaattt
    24181 attggtgtcg gtctcgatcc ttacaagctg caggcttgac aaatcacctg cctttggggg
    24241 gtgttcaaca gtggcacggt aaaagttaat tatgaaaacc gtcaatagta ggaaaagcat
    24301 caaaagcgct cccttcaata ttcccttttt tattttccgg tttttatgca tttcgtatct
    24361 tgtccaagac atattgtgtt cccagcatct cgccgcaggt gagcaccgat ccaattgtga
    24421 cgccgacgac tccgtgtaca ttcgtgttct gccctgtcag aaacagattc ggcaccttgg
    24481 ttctaggtat tatcagggtt tttaaaggac tgttgaaatc tttctgcagg ccatagaccg
    24541 atcccctgaa agtgcctgtg taatctcgat aagtcagcgg tgtggatgta tagtaggatt
    24601 tgatcttgcc cctgatgccc gggaaatcat ttgcaaccac ttcaaggagc ttttctgccc
    24661 tgccggtttt aaaatcaatg taatcatcac ccctttgttc tatccaggta ttttcccatg
    24721 gtgaaacctc gttccagttc atataggctg taacgataac tgaaccggtg tatttttcat
    24781 ttttggatcc tggggaaaaa tgcatcatgt agctttcagg ccatcttccc ggagcgatcc
    24841 tgtcgccgtg ccagacatcg tcggttcggt agatataata atggctgttt atgtattcaa
    24901 atgagttttc cttcattgca aggtacagtg agaacatgcc gtaggtatca tcgatacttg
    24961 caatcctttt ctgataggcg ggtctgacag gtgcattctc catcagggaa agcatcgctt
    25021 taggatggat atttgatata aaccattttg cggagagttc ttccgaatcg tttatcacaa
    25081 cgcttttcat caatcctgag ctgaaaagga attttgtcac ccgcgcattc ctcattattg
    25141 tacctccgtt ctcaactatg ccgcccgaca gaaggtcgga aacctgtgag cccccgtcaa
    25201 caaacctgta ggcgctgtcg acatatgttg agtttatgat cattggtgta tacaacggtg
    25261 tccggtcgcg cactcccgca tacagcggcg agttccctgt cagcaagctt ttcagttcac
    25321 ggttgccggt gagtgaatcg agataatcat tgaacccggt gctgaaataa ttgaaatatc
    25381 ccggccgctt cccggggagg gtatccatgt tgagaagatc gactgacctg cttacctcct
    25441 gcattttcgc aacgtaattc ctgactgcct ctgactcccc cggaaaatac gaaagcagct
    25501 tttccgaaaa atggtcatac ccgttggcaa aactgaattc cctgtcgcct aatctgatga
    25561 tttcataggc atttgtatcc atcttcctga ttttcagctt tccacagagt ctgaaatatt
    25621 tcaggaattt gtgaagcaat tgtccgtctt caacgcttcc tatgtaatgc atgccagtat
    25681 caaatacgga tccttttctt ttaaaagtct gaaggcatcc tcccagctgc ttatttttct
    25741 caatcaggca aaccttaaat ccttcccggc tcattatgta ggcacactga aggcctccga
    25801 gcccgctacc cactataata acatcatact tcatccagac ttattctaat aatatgattc
    25861 aacactgagg cttaactttt gtttcctcgg acattctgct tctgccggtc cctgaaaacc
    25921 tttgcttttc cttaagcact gaatctcaac tgtatattcg cggaacttaa taaaacccgg
    25981 aatattaaaa aacagcctca aagttaaaat aggattatag ataaatactg ctatttttga
    26041 aaaaaatgaa cctgatgttc agagaagaag attttgagga tattcgtcct taccatgatg
    26101 atgaaataaa tccggccctt aggcggataa tatccaatcc ggtttttgat acgatcctgg
    26161 atttcttttt cccctatgat gagaaggaca agatcaggaa attactctct gaaaccagat
    26221 ccgtgtattc gtttcagaaa aattttgtcg accgactggt gagagccatt gtttccaaaa
    26281 gttcttccgg cctgacctgc agcggatttg aaaatctcac gccgggaaaa gcatatcttt
    26341 tcatctcaaa tcacagggat attgtcctcg attcggccat tctccagata ctgttgtttg
    26401 aacacgggca caatacctcc gaaataacct tcggcagcaa ccttatggtc aatcagtttg
    26461 taatcgacct gggaaagtcc aacagaatgt tcagggttgt acgcggtggt tcgagcaggg
    26521 aactcctgca taactcgcag caactttcag cttatatcag gtacaccata gtaagcaaag
    26581 ggatttcagt atggatagcc cagcggcccg gccggacaaa aaacgggaat gacaggacgg
    26641 aaaccagcct gctcaaaatg ttaaacatga gcggccgagg gaagcccgag gattccctgc
    26701 atgaacttaa cattgtacca tgcacggtct catatgaata tgagccctgt tgtgccctga
    26761 aaataaagga gcttattgct acatcgctca ccggaaccta ccaaaagcaa cccggtgagg
    26821 acctcaacag cattataaaa ggaattatgc agcaaaaggg caggattcac tttgcaatag
    26881 gatctcccct tgacaaaagt ctcggtcagc tcagtaatga gtcaaccttc aacaggaaaa
    26941 taaataccct cgcggccata attgacaaag aaatgtacag gaatttcagg ctgtggccca
    27001 acaactacat ttcatgggat attcagaacc attccgggag attccgggag ttttatactc
    27061 cggatgacaa ggcaaaattc ctggaatata tggaaaatga aactggcgga ctgacgggtg
    27121 acagaaacat gataaatgat ctgttcctgc agatctatgc caacccggtc tcaaactatc
    27181 ttactcttaa caggtaaaca gctgccacct cagcgctgtt ttgcgataaa tgcagcaagc
    27241 gatttgccaa gtttggcata ggcctcttca atccggtaac ctgtatcaaa atcataacca
    27301 agggcatccc ttccggggca gttcctgacc tcaatcactg ctgtttcatc gcccgaggaa
    27361 atattcctga acgtaatgac caggtcaata tatgcgggct tccttgttat cccgatattg
    27421 aagccgggtt cggtgaatgt ggtatggata ttcatctcaa accgggcatc tgtaacatcg
    27481 cgggtaccgg tcagaccggt tttttcagca tccctgttga acagctgctc gaatttcggt
    27541 tcatatctcg aagccctgtc acctttccat gcttccttcc atttttcccc ctttcctgcc
    27601 tcggatttgt tgtaatcctc aacctttttt gcaatgtagt catcttccct gtcaaactta
    27661 cctacggcca tattgctgta atcaaacgtg accaggagcg atttctctcc ctttaaaaat
    27721 ccgagattcc ctttctttag ggtgaatttc tgtgcatgtg atattgatcc ggcaatcagg
    27781 acctgaatca ggataacgga aagcaatttt ttcatcttgg tatttattaa ttatataaag
    27841 ataaatattt acatgcttaa aaacattaat ttgccataat tgttcgtcag ctatattttt
    27901 aactatcaac gaaaaaatcc gtgagaatgt atctgaaagc cggtaaaaag ttatttctct
    27961 ccatatacct gcttttccat tctttcgtgt ttacaactga ggcccaaaca attgtcaggg
    28021 gaagggtaac tgatttgcag acttttgaac ccgtggtctt tgccaacatt gttttcaagg
    28081 gaacaaccat cggaactcag tcggggtttg acggaagcta cgttctcagc gggattaccg
    28141 ggagtgactc cgtggttatc tcatttgtgg gatacactac aaaaactatc ggaataatcc
    28201 cgggcagcga tcaggatatt gacatacgtc tcagcccggc catattttca attggtgagg
    28261 taaccgtcag acctggatta aatcctgcaa taaagctgct cagggaggtc tggaaaaaca
    28321 tacctgaaaa ccatatagac aagcttcagt cctatcagta tgtaaattac tcaaggtcaa
    28381 cattatatat aagaaaaccc ggatactaca aaaagaccgg caggaatctt gaaaagcctc
    28441 attcagagag atttgaaaag tactcggtaa aaaccggtga agaggatatt cccgcaatac
    28501 cttcctattt cactgaaata ctgagtgaaa attttcacct gaaatcaaca gggcagaatt
    28561 tcattcatat catagggtcc aattctgaag ggatcgcctt tgagacaacg agtcttccgg
    28621 cccagctgat cacaaagcag gagaatttca atttcatgga taacacggta cttattatcg
    28681 accggtcatt tatttctccc ctgtcccgat cgggcctttt ctattataag tactacctca
    28741 gggataccat gctcatcgac aacagatatc agtgtttcag ggttgatttt attccgaaaa
    28801 gggaagaaga tcctgttttc cggggcagct tctggatcaa tgatacaacc tttgccctga
    28861 aaaggatctc tgttgaggtc ggggagaagg ctgaactgaa tttcatcagg agggtgaaga
    28921 tccagcagga ctacgaaccc gccggtggcg gggcctggtt tcccgtcagc acacgattta
    28981 tggccgatgc agccaacctg tttctcacca atttttcccg gaaaacagaa ataatcgtta
    29041 ataatccgaa gcagcccgga ttttactcgt cggagctcaa ggtcagtttt gatgtcaata
    29101 accgggatcc cggattctgg gaaaaatcgc ggatgaattc acttgaaaaa actgacagcc
    29161 tggcacttca gaagatcggg atactaaaag agaacagcgg gataagcatc gctgcaaaac
    29221 tcattgaagc gtcaataaga ggttattacg atttcgggaa atttgaagcg ggtccctacc
    29281 ttctttttta tggccgcaat gacacggaag gcagcaggtt caggatcggg ggccggacaa
    29341 attcccgctt cagcaatcgc cttacccttg aaggttacac tgctttcggc acaagggaca
    29401 aaaggatgaa aggatccgta caggctgctt acctgttgtc ggagaaatac tggacctctg
    29461 tcggtctgca actgcgtgac gacctcgagt acgccggggc aatcgatgag ttctattcac
    29521 agaacagctt cctgactttt gcttccacct tcggaggatc ggacatgatg gccagaacga
    29581 caataatcag gccatggatc ggctccgaca tcaccagggg actccaggca aagatggtgc
    29641 tgacccgcaa aacattcgag ccggcaggga aggaatatca ttttgcatgg tatgcaggta
    29701 aggacggaac cggcttgaaa gataatttca tgacaaatga actgggactt atactcagct
    29761 atcagcccgg ggcggtatat gtcctggacg gtaacaggag gtttgccgtc aatttcaatc
    29821 agtatcccgt tttcagtctg gaatatttca ggggatttaa aaatccggga aaaggtgatt
    29881 tcagctatga caggatctcg gccggtattg accacaggtt caatcccgga ggactgggaa
    29941 ccgttgactg gaatgcaaga tattcaaagg tctttggagc tctgccctat ccactgctta
    30001 cgacatttgc cgggaacgag tcaatcttca gggccgacag aacctgtaac ctgatgaacc
    30061 ttggcgaatt cgtggccgat gaatctcttg agcttttcct ttcttatcat atgaacgggt
    30121 tgctgcttgg aaaactgccg ctgataaaga agcttgaatg gaggacggtt ttttccggaa
    30181 gggttgcata cggctcattc aatgaaaagt caaatggttt ctatgatcct gaatccaatc
    30241 ctgccggaat actgtcaaag cttgatcctg ccggatatcc tgtcagtgaa ttcagcaccc
    30301 tgtcatggcg gcatccttac gcggaaattt cctatggaat agagaatatt ttcaggtttt
    30361 tcaggatcga cctgatccaa cgcctgacct ggcttgataa cccgggaagc cggaggttgg
    30421 ccgtaaagat aagcggtgtt ttcaggttct gaaaacgatt gttgctccga gtcaccggct
    30481 aaggacaaac agtttatttg atgtaagccg tgaattgtca atcacttcaa gctgcatgtt
    30541 atatcctgat gccagctcac tgatgaatct ccctgaaaag aagaacagtc tcctgtcaag
    30601 gcttttatta tatccgaatg tggttgaaaa gaactctgtg aatcttgttc ccaggtgtcg
    30661 tttctgaagg tcgctgtcgg catcccggat tattattttt cctccgggtt ttagtcggcg
    30721 gatgcagctt gccagcaatc ctgcctgtct ttcgcgggga acatagtgca gaacatcgct
    30781 gaggaggaaa acatcggcat ccggataatc atatgttacg gcgtcggcag ccacaaagct
    30841 tattctgtca ttcttcgaga tgcagttgtt tgccagctct attttgttct cgtcataatc
    30901 gatgccgagg atgttcctgt tttccgatgt gaatcccagc atgtaagata taataccgta
    30961 tccgcagccg atatcgacaa cttgtgcatt ttcgggcacc aggctgttta tatattcata
    31021 gttcctttcc agcctgagtt taatcctggt ataccattca agcaccggcc ccttgtagat
    31081 atagttgctt acaagcctgt tcctgaaata tgatggtgtt tccagttctt tccttagaac
    31141 tttgtattct tccctgtaaa acctgagaaa tgtccttgtg cgctgatggt agtcgtgccc
    31201 aaagtcagta tccgttgctg ccaccctggg gtatattttc aacgtgacgc tgccttttct
    31261 gagatgattt tctccctttc tcatacagtc acctgctccg tgaatgagta tgggagtaat
    31321 gtccagtcca agcttttcag ccatcaaaaa tgctcccttg tgaaaacgaa ggatctccga
    31381 gtcaggtgac cgggttccct cgggaaaaac caggatcgaa taaccttccc cgatttcctt
    31441 ttcaagcttc tcaatcaggt tttcatatcc ccacgatacc gggtagaagt ggagatatct
    31501 gataacaagg gcataaagcg gattgttcca gacccagttg tttgtgagta taattatttt
    31561 cggattgagc atcagcagca gggggaggtc gagatgtgac tgatgattcg atataataag
    31621 cgaaggtctg gaaaaatcgg cattttcacg attcaatatg gttttcctga tgcctgtaag
    31681 tccgtaaacc ggtaatctga tgcaccacat cagcgcatga tgcatgaact ttctcttttt
    31741 cttcccggaa acagggacca ggaggacagc aataagcagg atgttaagaa gaacacacag
    31801 aaccagacct gcggtcagtg taacaactgt cagcagaact tcagaaattg tcatgggaag
    31861 atagcggttc ttaccttttt tcctgaccag ccagttgaaa actactatct caaaagtata
    31921 ggagatcagt accaccgaaa gcaatcccac gatcgatatc agtgctatgg atttcagtgc
    31981 aggatgtttt gcaagtatca atacaccggt tccggaaata gatatcagga cggacagcag
    32041 aatgcatgtc ttgtatgata ggaggttcct tgtcccggtc ttgtatccct ggaccaatcc
    32101 ctgcattatg taaatgctgt aatcgatccc cagcccggtg atgaactggg aaatgatgat
    32161 gttgaatatg ttgaatttga tacctgtgat tcccatgaac ccgagtgtcc agaaccagct
    32221 tacaagaatg ggaatgaagg tgatgaaacc aagctcaaac cggccaaaga ctattatcat
    32281 tattaccagt atgatcgaca ggctgacatt ggctatgaaa tcaaagtctc ttcccagcac
    32341 tcttaccact tccgacaata ttgatttcct gtcaatcaca accactccat caattttgcc
    32401 tatttcctcc tcaacataat ccttgttcct tgcatctgtt ttcattaccg aaactatggc
    32461 ggtcagaccg ggagcataag tgatgttttc ggagtaaaaa tgatcggcca gctttcgaaa
    32521 gtcaccgggg tccatcagcg tatactttct gtcaagcaaa ttgtaaaact cttcaaaagc
    32581 atcctgccgg aagcctgttc ttgcagaata atatctgaaa tttttcagga aagaatcctt
    32641 tttattcctg ttccagaaaa gattccatct ttcaattcgc tttttctgaa gcgaatcgga
    32701 cgataagagg gcgccgggtg aattgaaggt tcttatcctg ttttctgcca tcatggtttt
    32761 tccttctgcc atcagtttct cattgttcac gagggcttcc tgcaggtctt cgccatgagc
    32821 tattacataa accgaattga gcttgtaatt attgatcctg ttcagattct cctcggctat
    32881 ggcaagtttc ttcgacatgt aattcatttt catcatatcg ctttcgaaac ccactttgta
    32941 aaaatagaac cagctgacca ccgttgctat taccatgaag agaagaagtc ttttattctt
    33001 atcgaattca taattggtca ccttgtctag aattcgctcg aggtagtttt tttcctctgt
    33061 ttcatcatcc tctctcctgg taacatccag tatctgcggc attacgatca ttgtaaagaa
    33121 ggctgcggta atcacgctta tggctgcaag gatgcctagc tcatgcaatg actcggagga
    33181 gacaagaaca aggctcatga actcaaaggc agttgtaaaa ccgcagacga ttatcggaaa
    33241 ggatacatcc ctgatcgttt cggccactga atgtttatgc ttgtaatgag ccgtaatatg
    33301 caaggcatag tcgacggtaa ttgcaaggat aaccgtgccg atgctcaggg ctattatgga
    33361 tatttttgcc tgaacgagat aaataactgc aagggcagtg agcccgccaa aaactgcagg
    33421 cagaaaaata aaaggtatca gtgccttctt ttttattgag aaggtaatga tcaggaacag
    33481 aaggattatc gttatggtta ttgtcaggct gatatctctt ttcagcctgt cggcgttgcc
    33541 ggctgccacc gctgccgaac caaaatactc gatcctgatc tgactgcctg cttccctcga
    33601 tattgcattt ataacaccgt caagttcctt gatcagcctg gatgtttttg aagtgacatt
    33661 tgaagagtat gtcgtggtca ggaacatcag aaggctcttt ttatccctcg aaaaaatcga
    33721 tccgtcaatt atcaggtagt tgatgtcatt ttgaaaggct ctgagcttcc tggtgacgat
    33781 acttgtcagc cctgccggat ccatgagtat atttcgtttc atggcgaagg atgcgggtgt
    33841 aagcaggctg ttgtaactgt tctcaagtga ttccctgatc ttttccggtg ctatcagcga
    33901 atcaagcgca acatagtcct cttcctccag gaaaagagga agattcctgt aaacgattgc
    33961 cattacatct tccatgatat tatcaggtat cctgaatgtg acgtcactca ggtactgggg
    34021 aaaatccccg gtctgcaggg tgtcatagag cttttttcct accaggatca gcctatcggg
    34081 agccggcgga gcagaggtat cggcaagcga gatatttact atgatcttgt caagaaattt
    34141 cgaacggttg aatattgttg ttatttcagt tttgcggtca acacccgaga tcatctttgc
    34201 aatgtcttcc tcaaaactga tccttgatgc gagcagtgcg gcaatcagca gtattgccgt
    34261 tacaatcgtg aagaacagta tcctcctgct cttgaaataa ccgaacagaa aaagaaagaa
    34321 gttttccatt tacatactca atatcaccgg tttcctcctc acaagtttca gaaccacgaa
    34381 tgaaaccagt ccggcaaaag cagcaaaaac ggctgcgaat ataaaagcgc ctgtaatata
    34441 ctgcaacaaa ttgttctcaa aagtcttcag cgtgatttct ccgctgaagc tgatccggct
    34501 tcccgttccg agtacgatcc ctcccgttgc atagctcagg tacaggatca gggggatcat
    34561 cgggggtatg ctgatattgg cagcaacggc aaccaggaac ttgctcagcc tgaaaatatg
    34621 ggcaagagcc aatgccgtta caagttgaaa accccagatc ggaacaatac ccataaatat
    34681 tccgaatgca atggcgagcg agattttcag atttgtttcc cgggtaagca ggatatgttt
    34741 attcacaaat tctttaatct tttccttgtt cagattccgt agggatgaaa acggtatgat
    34801 gtagaatacg gccgccagta caagacaggt attcagcagg ctgatccttg caaagtccct
    34861 gaaaaacctg taatgtgaaa cacgttcctc cgcaggaggg tagtaaaccc gcaccggcac
    34921 gctagaaatt tttattcctt tccaggctgc ccttatcagg atctcggttt caaattcata
    34981 cttaacggta aagaatctca ttgatttgag tggcccgagg ggatatagcc tgaatccggt
    35041 ctgagtatca ttcagcttta ttcctgtaag aaatctgaac cagaaattgg agaatttgtt
    35101 tgcaaaccgg cttccgcgtg agatattttt tactccaaga ttacggtcgc ctgtgatcag
    35161 gctgtccggt gaattttcca cggcggcaat aaaggatggt atgtcagccg gatcatgctg
    35221 tccgtcggaa tcgatggtta taacataatc atatccttcc ttaatggcca ggtcaagtcc
    35281 ctgtcgtatg gcataacctt tgcccctgtt aacctggtat gcaataactt tcagggaacc
    35341 attgacgaga atgacattac tgaggatctg tgccgtcctg tcggtcgatc cgtcgttgac
    35401 aacaatgata ttctcagtga attcacgtac tccgtttatt actttttcaa gtgttgaatc
    35461 gttattgtat gtcggaatta taacacaata cctcatccct gcaaactgcg ttttctgaag
    35521 gaatccgcat gagtgatttc agatacggtc cggcagtcgc tatccctttc acccggagcc
    35581 gcgggcccgc ccaaatcaat catgtttgtc cccttcattc cattataagc catgatcata
    35641 ccatggttaa atccgggttg aacctcttct cctgtttgta cagactggtt tcttttttcc
    35701 gggggttgca agttaattaa aaaatcagat tatgatctgg attgttccct ttgaagattt
    35761 ggtttaatag tttgtttttt ttactttgcg caaatttttt caggcccgat aaacctttaa
    35821 ttaagctttc agataaattt ggttttatgg tggtgattgg cgaacatttt cttacgacgg
    35881 aggatttcaa gaagcttttg tacggtggag aaatgattga aatatcctca gaggcaaaag
    35941 gcagggtatc cagaagtttc gatttcctac ggtcatttca cagggataag ataatttatg
    36001 gcataaatac cggattggga cccatggccc agtacaggat agaggatgag gaccggatca
    36061 acctgcagta taatgccata aggagtcatg cctccggatg tggtcagccg gtggatcctg
    36121 tttatgtaag atcgtcactt atcgtgcttc tgaacaactt cctgcagggt cattccggaa
    36181 tccaccccga ggtggttgag ctgatcagcc ggttcataaa caacgatata tcgcccgtgg
    36241 taccggaaca tggcggcgtt ggtgccagcg gcgatctggt acagcttgcc catatagctc
    36301 tggccctgat cggtgaaggt gatgtaaact tcggaggccg ggtcattccc gctgcagagg
    36361 ccatgagaaa aagcggactt gagcccatta aggtccacat cagggaagga ctttccctga
    36421 tcaatggcac ctgtttcatg accggtacgg ggatggtgaa tataatccat gcaaggaatc
    36481 tggtttcatg ggcgctgatc gcaagcgcaa tgattaatga aatagtgcat tcatgcgacg
    36541 acagcttttc aaaggaactg aatgaagtga aaaagcatcc cggccagcgt gatgttgcaa
    36601 gggttatcag ggaaatactg gcagacagca atctcatccg caaacgtgag aatgagtttt
    36661 ataacaataa ccacaatcac acgaaaaaaa ttcttaacga caaggtccag gaatattatt
    36721 caataagatg cgttccccag attgcaggac cggtccttga cacaatcaga gaatcggaaa
    36781 aggtccttct taacgaggca aattcatcat gcgacaatcc gatgattgat gatgaaaacc
    36841 tgaacattta tcacggaggc aacttccatg gtgattatgt gtcgtttgaa atggacaagg
    36901 taaagatagc ggtggcaaaa ttatcgctcc tgatcgacag gcagatcaac tatcttatga
    36961 atgataagct gaataacatt cttcctcctt ttgtcaacct gggaaagctc ggcgttaatt
    37021 tcggcatgca gggtgcccag tttacctcaa catctacggt tgcggaaaac cagtcggttt
    37081 catttccgaa ttatcttcac agcattccca acaacaacga taaccaggat attgtcagca
    37141 tgggaacaaa ctcggccctc ctggccagaa gggtgatcga caatacctac caggtgctcg
    37201 ccattcagat gctgagcctt gtacaggcaa tagattatct caggattgag gataatctgt
    37261 cttcacggac cagacaggct tatcgccaga tacgggatat tgtgcccgtg tttgttgaag
    37321 acactgttaa atacccggaa ataaggctga tcaaggattt tcttttcagc aggctcctga
    37381 gtttttttga ttaaaacctt tataagcagg atgaaatacg cgcttgtaac cggagggtcg
    37441 agaggaattg gaagggcagt gtgtacgggc cttgttccgc tgggatatca tgttatcatc
    37501 aattacagga gcaatgatgc cgaggctgaa aaaacccttg caatgattaa tcaggcagga
    37561 ggcaccgggg agcttatgaa gtttgatgtt tctgactccc gccaggttga cggggcactg
    37621 aacaggtgga atgaacaaca cggcgatgac tatattgaag tgcttgtcaa taatgccggc
    37681 atcaggaagg atatgctcat gatgtggatg gaaaacgaca gctggaggga agtcctttca
    37741 accaatctcg acggattctt ttatgtcacc cgggcattac tgaagaacat gcttgtacgg
    37801 aaatacggaa ggatcataaa catcgtttcc ttatcgggaa taaagggaat gcccgggcag
    37861 gtcaattact ctgcggctaa agcagcaata atcggagcaa caaaggccct tgcgcaggag
    37921 atcgggaaaa agaacgtgac tgtcaatgca gttgcacccg gcttcataag aaccgacatg
    37981 accagtgaac tgaatgaaaa cgaactgaag ggtttaattc ccctgaacag gt
//
LOCUS       DAOOYG010000014        36874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_949_length_36874_cov_7.550667, whole genome shotgun sequence.
ACCESSION   DAOOYG010000014 DAOOYG010000000
VERSION     DAOOYG010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 36874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36874
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_949_length_36874_cov_7.550667"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PK288_02550"
     CDS             complement(<1..969)
                     /locus_tag="PK288_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HPM17827.1"
                     /translation="MGRYYSNILTWLLVIIFAGMQYLFSQEITDPEQEYLRIKEMAFS
                     GNLEEAGSAARKLLKEYPSYGDTRVLLARILAWQKEFAEAEAVIDTLLMTEPQNTDAL
                     EVKRDISQWSKTKPRVSTDLRAGYAFDYFKVPYSRFWQVFRAGAGHRFNWGTAAAYVN
                     IGNIALGEDPVIHATEPQFEAEAYPVLSKKNYAYLDYAYSPGSYFPTHRAAVELWQAL
                     PAGWAVSTGLNYYYFDRNVFIALVSVEKYIRKYWFSGKCYVYFKDNGPTTSFYVNARR
                     YFNDTDYLQLTLGTGTAPDEPFDIQSDLERLSANSIRLAYNFAVSDR"
     gene            complement(970..1332)
                     /locus_tag="PK288_02555"
     CDS             complement(970..1332)
                     /locus_tag="PK288_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM17828.1"
                     /translation="MKILICEDNPLALKTLSVILEKEGFETSVAEDGNRAIELLEGND
                     YDLLLVDIHLPFHSGLELVKYLRSDQGKDTPVLILTAFSDPRMQREAGELGIDGYIVK
                     PFNPSELISKVRSIFKKG"
     gene            complement(1737..2606)
                     /locus_tag="PK288_02560"
     CDS             complement(1737..2606)
                     /locus_tag="PK288_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17829.1"
                     /translation="MNYPAIFLYLITSLLFIGCSKEDEPIPPTAVDAYVVADHNSIRL
                     ASIPSQWISEAKRKLNIAYSHTSHGSQITDGMSGLVSFKGSSFSWSGKKSDDALFIHD
                     YAISGDLGNPDRTTWAARTRTYLAEHPEINVVMWSWCGQVSTASVSDINKYLQLMEEL
                     EKDFPGVTFVYMTGHLDGTGLTGNLHLRNEQIRNYCRNNKKVLYDFADIESYNPDGVY
                     FGNKKPNDACAYDTNGDGTLDGNWAIEWQNSHKEGQDWYKCNSAHSQPVNANMKAYAA
                     WWLWARLAGWDGK"
     gene            complement(2892..3278)
                     /locus_tag="PK288_02565"
     CDS             complement(2892..3278)
                     /locus_tag="PK288_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17830.1"
                     /translation="MSNGVINLSPRQSYDLSRKGAVIVDVRESYMNSFKMFNVEKVIY
                     MPFSGLQKSYPDLPVDKPLIFADVNGSKSRESVLFMYSKGYENVASMAGGMDAWEREG
                     LPLTNGKSVRTPGFRTDGRKTGFRIR"
     gene            complement(3421..3798)
                     /locus_tag="PK288_02570"
     CDS             complement(3421..3798)
                     /locus_tag="PK288_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPM17831.1"
                     /translation="MGKGKKGLFDKADVKLARFARALAHPARIAIIRLLAAREACCFN
                     EISKAIPLADSTVSQHLAELRKAGLINASPDLPRIRYSISNGDLKAARKLLKDIAKTG
                     SKKHEPRMNDVRHNYAAELSQAR"
     gene            complement(3911..4603)
                     /locus_tag="PK288_02575"
     CDS             complement(3911..4603)
                     /locus_tag="PK288_02575"
                     /inference="COORDINATES: protein motif:HMM:NF027590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HPM17832.1"
                     /translation="MLTFADKAILFYGSLKYDGSLPDGISVMNPFTEAEVFRAVSEFY
                     RKFYSDPGPRRMIIGINPGRFGAGLTGIPFTDSVRLKEKCGLSIPGIKSYEISSVFMY
                     EMIDGYGGPGKFYGDYYITSVSPLGFTSRNEKGREVNFNYYDNRNLEESIEEFAVASI
                     RKQLDFGIERNICFCLGAGKNYRYLSRLNQKHHFFDHIEPLEHPRFIMQYKSKHKDAC
                     ISDYLQKLKKSE"
     gene            4709..5173
                     /locus_tag="PK288_02580"
     CDS             4709..5173
                     /locus_tag="PK288_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPM17833.1"
                     /translation="MNDLLRPYGDKQKTFDKRYLQMAQIWAGNSYCIRRQVGALIVKG
                     RMIISDGYNGTPSGFENVCEDENNVTKPYVLHAEANAITKVAKSNNSSEDATLYVTTS
                     PCMECSKLIIQSGIKRVVYCNRYHNTDGLDLLKRAGIELVYIDSENDIYEVQ"
     gene            5160..6764
                     /locus_tag="PK288_02585"
     CDS             5160..6764
                     /locus_tag="PK288_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPM17834.1"
                     /translation="MKYNNTNRSIYLPVLFAVTLIAGILTGIYFPGRNRNQVQPVLRA
                     RHDKINSVLNIIEADYVDSINRNDLVEAAIPAILKKLDPHSVYIPAKDLARANEPLQG
                     NFDGIGISFNLLTDTILIISTIPGGPSEKIGLLPGDKIIYVNDSLVAGKQISEERVMG
                     MLKGPRGTIVNIKVLRKGVGDLLPFEIRRDKIPINSVEVSYMLNSNTGYIKIINFAIT
                     TFDEFQRSMNELRKEGMTRLILDLRQNSGGVMESAIRIADQFLEKGQLIVFTKGRTQP
                     KNEARASGKGEFRSGDLIVLIDEWSASASEILAGAVQDNDRGTIIGRRSFGKGLVQEP
                     VSFADGSGIRLTIARYYTPTGRSIQKPYNNGFDDYFDDLNSRFRHGEFEIPDSIHFAD
                     SLKFITPGGRVVYGGGGIMPDVFVPLDTSGISPYFLAVRPQIYRFALKYTENNREKLQ
                     KFTETTDLEKYLNSQRLMEQFTEFVAGTGVKKDPEGLKISGLLIHTQLKAYIARNILD
                     NKGFYPILDDIDNTLQVAIKYMEKNK"
     gene            complement(6816..7619)
                     /locus_tag="PK288_02590"
     CDS             complement(6816..7619)
                     /locus_tag="PK288_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPM17835.1"
                     /translation="MKYYILHNGGKIYYSDSGMGSPVILIHGHLESSEIWERFAGRLS
                     ALHRVISVDLPGHGRSAVYGDCHTTEFMAEAVKSLLDSLGIRKAFLIGHSMGGYVTLA
                     FLELFPELLTGYCLFHSHPFADTPETIQKRENEIKIVQSGKKYLMYPENISRMFATMN
                     MEKFREALQNSKNIASRIRDEGIIAALNGMMIRPDRVSVMEKGSAGCLWILGRHDNYI
                     PFDQIHKRVKLPANARIAILENSGHLGFIEEEDQSFQIVTEFIRDLQNG"
     gene            7779..8423
                     /locus_tag="PK288_02595"
     CDS             7779..8423
                     /locus_tag="PK288_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPM17836.1"
                     /translation="MNKVLFSTISKKFIMALAGLFLLMFLPVHLAVNLMLLKPDPEPF
                     NKAARFMATFPLIRIMEIVLFAGIVIHITTGLIIQIQNWLARPVGYAGGSKSETSFFS
                     RYMIWTGASIMIFLVLHFFNFYFIRLGLVPGNPDDFYTVAHNLFSIPAYNYIYLSCFL
                     LLGLHLYHAFSSAFQTLGLNHRIWTPVIRIISLIYSIAIPLGFGIISIIIWKFR"
     gene            8438..10357
                     /locus_tag="PK288_02600"
     CDS             8438..10357
                     /locus_tag="PK288_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPM17837.1"
                     /translation="MNTLDSKIPPGPLAQKWTRFKNEVRLVSPANKRKLEIIVVGTGI
                     SGAGAASALGELGYRVKNFCYQDSPRRAHSVAAQGGINAAKNYQNDGDSVYRLFYDMI
                     KGGDFRAREANVYRAAEVSNQVIDHFTALGVPFARDYGGTLDTRSFGGVQVSRTFYSK
                     GQTGQQCLLGCYSALNRQIQKGNVQIFPRREMLDLVVVDGKARGIITRNLITGEIERH
                     SANAVVLATGGYGTVYYLSTLAVNSNASAAWKAHKKGAFFANPSFLQIHPTSVPQLNQ
                     YQSKLTLMSESLRNDGRLWVPLQKGDKRPPNGIPEEERDYFLERRYPAFGNLVPRDVA
                     SRAVKERCDAGYGVSETGLAVYLDFRDALKKQGRKAIENKYGNLFQMYKTITGIDPYE
                     EPMRIYPAGHYTMGGLWVDYELMTTIPGLYAIGEANFSDHGANRLGASSLMQAAGDGF
                     FILPVTISNYLAGEIRSAPVPLSHPEFEAAEKTAVARLNRLISIKGNQTAGSFHRRLG
                     KIMWDHCGMTRNEEGLKKALVLIRELRDEFWQNLNVPGTIYELNQELEKAGRVADYFE
                     LAELLVNDALERKESCGAHFREEYQTPDGEARRNDAEYAYVAAWEYTGENTPHVLHRE
                     ELKFEEVELRERSYK"
     gene            10457..11191
                     /locus_tag="PK288_02605"
     CDS             10457..11191
                     /locus_tag="PK288_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPM17838.1"
                     /translation="MKLTLKIWRQKDARAKGRFVTYELDDVSEKMAFLEMLDSLNRKL
                     VESDQEPVAFDHDCREGICGSCGIYINGHPHGPVPAITTCHLSMRYFNDGDTVWIEPW
                     RAKSFPVIRDLIVDRSAFDKIQAAGGYISVNTGSAPEANSIPIPRKNYDNAFDAAICI
                     GCGACVAACKNASAVLFVSAKITQLALLPQGRVEARDRVRAMVEKMDELGFGNCSNTG
                     ACEAECPKEIKIKSIAAMKREYYKAF"
     gene            11217..12938
                     /locus_tag="PK288_02610"
     CDS             11217..12938
                     /locus_tag="PK288_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM17839.1"
                     /translation="MNRKRFLGIAAASGAGLWINSGNFLKNGRLYASSPKPSVAKNNE
                     LGADLIIAGGGLGGCAAALSALRNGLTVIMTEETDWIGGQLTQQGVPPDEHQWIETQG
                     STKLYRDFRNMIRSYYFRNYPLTEKASANKFLNPGDGAVSRLCHEPRAALAVLNELLA
                     PWISTGRLILITNCKITAADVSGNIVRSLQASMGPGGRNIILSGRFFIDATELGDLLP
                     LTGTEFVTGTESRKDTRELHAPEKGDPDNQQAFTYCFAIDHIEGENHTIDKPEAYDFW
                     KNHVPELTPPWSGKLLDLRYSNPSTLEPKKLGFNPDGSGEGSILNLWNYRRIINRLNF
                     RPGAYASDITLVNWPQNDYMLGNLIGVGTAEHEKHLWNAKQLSLSLLYWLQTEAPRPG
                     KGTGWPGLRLRKDIMGTDDGLAKYPYVRESRRIRGVFTILEEHVGKENRGLITGNKSE
                     VRAAGFFDSVGTGYYHIDLHPSTGGNNYIDFPSLPFQIPLGALLPVRMDNLIPACKNI
                     ATTHITNGCYRLHPVEWTIGESAGMLAAFTLAKKVRPREVREKGQLLEEFQELIRSQG
                     IETHWEE"
     gene            12982..14073
                     /locus_tag="PK288_02615"
     CDS             12982..14073
                     /locus_tag="PK288_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HPM17840.1"
                     /translation="MKSIQTRRDFLGKSTLAGMAALSVPLTSCSDTDRPVEEKVARPP
                     EFLVPAGDAIPITGTFLDEISHDIPHQNWGEKEWDADFLNMKAIGIDTVIMIRSGYRK
                     FITYPSKYLLAKGCYMPSVDLLDMFLRLADKHGMNFYFGLYDSGKYWDTGDLSWEIED
                     NKYVIDEVWKDYGEKYKSFRGWYISGEISRKTKGAIGAFHALGKQCKDVSGGLPTFIS
                     PWIDGKKAVEAASGDITRAKAVSVIEHEREWGEIFDGIHDVVDACAFQDGHIDYDELD
                     AFFSVNKKLADKYGMKCWTNAETFDRDMPIRFFPIKFDKLRLKLEAAKRAGYEKGITF
                     EFSHFMSPQSAYLQAGHLYRRYKEYFGIA"
     gene            complement(14103..15491)
                     /locus_tag="PK288_02620"
     CDS             complement(14103..15491)
                     /locus_tag="PK288_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPM17841.1"
                     /translation="MFRREVYIKRRARLHGLMRSGLALFLGNTDSPMNYPANPYHFRQ
                     DSDFLYFFGLDLPDLAGVMDFDTGKDWIFGNDFDMDDIIWMGSQPSVRELAETAGTNF
                     TGPLSKLEAVIREAISKKRPIHFLPPYRSETRMTLGSLLKENPCQMKASASEKLIRAV
                     VMMRSIKEKVEIEEIEQALAITFEMHTTAMKMCRPGIKEQEIAGIIEGIAWAKGAGPA
                     FPVILSVNGQTLHNHDHGNILRKGRMMVTDAGAETGMHYASDITRTTPVGGKFSQKQR
                     DIYEVVLKANTESIRAAGPDMSNLELHLNACRIIASSLKDLGLMKGDVDEAVEAGAHA
                     LFMPHGLGHLMGLDVHDMESLGEDFVGYNDDVKRSSQFGLAFLRFALPFKNGHVLTVE
                     PGCYFIPQLIDRWKAEGRHKEFINYSKLEPWKDFGGIRIEDNILITEKGNRVLGKAVP
                     KTVKEIETLCSW"
     gene            complement(15624..16169)
                     /locus_tag="PK288_02625"
     CDS             complement(15624..16169)
                     /locus_tag="PK288_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPM17842.1"
                     /translation="MKNSTGNLENDHVHILRLIDVMEIIAGQDEPDASHIEEVVYLIR
                     NFADGLHHVKEEEILFPFLETRGFSSQSGPVAVMLHEHAAGRNFVKGISEGLELYRKG
                     NKAAAGDIARNMKGYSELLRSHISKENNVLFRLADQVMSEDDDSIILSKFNSVAKSRY
                     AEKGGDDFVARIGNLATKYGI"
     gene            16291..16701
                     /locus_tag="PK288_02630"
     CDS             16291..16701
                     /locus_tag="PK288_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPM17843.1"
                     /translation="MFNRETEYALRSLVYIKLQNSKNRRPGAVEIARETGAPHFYIAK
                     ILQRLAREGFIESVKGKGGGFYFDPGKPDLTLKELITFIEGDRSFSGCGFGLKQCDEN
                     NPCPFHEKYAPIRRSLDNLLSEETVQSLANGVRL"
     gene            16819..17697
                     /locus_tag="PK288_02635"
     CDS             16819..17697
                     /locus_tag="PK288_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM17844.1"
                     /translation="MAERFLKGAGWIEIAAISIYGAFVANRMEDPSRARRWRVITWTT
                     FSTVFFLQLLIGISGHEKFLMTGKLHLPVPMMIIGGPVFRGQISFMTILFLSTIILSG
                     PAWCSQLCYFGAFDNLASIGRTTKKPLRNKAALKSTFLILVITAALILRWLNVSIAIT
                     TIIAASFGIAGLAVMVFFSRRRKKMVHCILYCPAGLMVNLLRHINPFRMYIDQSCTHC
                     MLCIKYCKYDALNESDINNKRPGFTCTMCGDCLAGCRHNSIKYKFLSLKPENARNLYI
                     IVTITLHSVFLALARI"
     gene            17887..18621
                     /locus_tag="PK288_02640"
     CDS             17887..18621
                     /locus_tag="PK288_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17845.1"
                     /translation="MPYRRLPNTDAARIRAMKIALEKGRNVHPKQMPFSGKLMVRLHK
                     FLPLFENMIQLQRQSYAAQYNKSRDYSEIVRKARLYLTHFVKVMNMAIYRGEISPEIR
                     SYYGLATNESGVPSLNTENELISWGKRIIEGEEYRLRKGGSPITNPTIAVVKVRYQNF
                     LDAWTFQKTLAKRTMDYVEKNSVLRKEADEIIQQLWNEIEESHSILPDDKRIPANEEY
                     GVVYFYRKNELNDIPAESGSGDKVWK"
     gene            18632..19645
                     /gene="dusB"
                     /locus_tag="PK288_02645"
     CDS             18632..19645
                     /gene="dusB"
                     /locus_tag="PK288_02645"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPM17846.1"
                     /translation="MKIGNITLAERPLFLAPMEDVTDPSFRLVCKKFGADFVYTEFIS
                     SDGLIRDGEKSVKKLEIYDHERPIGIQLYGHLPGAMVEAAVIAEEAGPELIDINFGCP
                     VKKIANRGAGAGMLKNIPLMVEMTRMIVDSVRLPVTVKTRTGWDDSSRNIVEVSEMLQ
                     DAGIKAITIHGRTRAQMYRGTADWSIIGEVKKNPRLRIPVIGNGDINHPVKAKEMFDR
                     YGVDGIMIGRGAVGRPWIFRDIRHFLDKGELLPEPTVSEKAVLALFHFEKSLEFKSGK
                     RAILEMRRHLSNYFKGLPGFRETRLKLLTSLEPSEIKDLIRDIGEKWGDYRTDDRTSV
                     YGE"
     gene            complement(19606..20586)
                     /locus_tag="PK288_02650"
     CDS             complement(19606..20586)
                     /locus_tag="PK288_02650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPM17847.1"
                     /translation="MIHLRSSFAGKNSFWRYIVMFAAVLIISNTLGAIPLLIAYALRA
                     ASDTGVISGIAEHPGDLSILGLDPNLGLILMLFPFVAGLIAFTLLVKPLNGRTLAMTI
                     NGNRNFRWKRFFVSGLVWFILSAVYLVVYLKMDPSNFTLNNTSRTLFFLIIISLVLVP
                     FQAAFEEVLFRGYLMQGFTVLAGNKWVPLVMTSLLFGLLHSFNPEVEKFGFMTMMPQY
                     FMFGIIFGIVTIMDDGIEAAIGAHAANNIFLCIMLTHDSSSLQTEAVFRQSNIYPWAE
                     FSALLISGIVFLVILRILFRWGSFSDLSGRVEKPEPGTHSPYTDVLSSVR"
     gene            complement(20598..22175)
                     /gene="nadB"
                     /locus_tag="PK288_02655"
     CDS             complement(20598..22175)
                     /gene="nadB"
                     /locus_tag="PK288_02655"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPM17848.1"
                     /translation="MRKKTDFLVIGSGIAGLTFAIKAAQYGKVSVITKSVLDDTNTRY
                     AQGGIAAVFSEPDNFEKHIRDTLIAGDGLCNEEVVRLVVQEAPERIKDLIDFGVPFDK
                     KEDGTYDLAREGGHTEPRIFHAKDKTGEAIEETLIDKVRRDPNIEIFENHFAIEILTQ
                     HHLGIEIKKNSAGVKCYGAYVADLVGQRVHTFLSRITVIATGGLGNVYQTTTNPEIAT
                     GDGVAMVYRAKGVVENMEFIQFHPTSLYDPEKKPSFLITEALRGYGAILKNIAGEKFM
                     NRYDSRGSLAPRDIVARAIDNELKIWGEDHVWLDCTHLDPEGLRNHFPNVYEHCLQRG
                     IDITRDMIPVVPCVHYSCGGIKVDIDGQSSIDRLYALGEASSTGLHGANRLASNSLIE
                     AVVFSHRAVMSSAKRIGELDFEDSIPEWDYKGTTHLEEMVLITQSLKEVQTIMSNYVG
                     IVRSDLRLNRAMVRLEILYRETESLYKKSLISKKICELRNLINVGYLIIKMAMNRKES
                     IGLHYSIDYPKKTDRGI"
     gene            22354..23904
                     /locus_tag="PK288_02660"
     CDS             22354..23904
                     /locus_tag="PK288_02660"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPM17849.1"
                     /translation="MAQEDVFRKLVAHCKEYGYVFPSSEIYDGLSAVYDYGQYGVELK
                     NNIRKYWWDSMVLLNDNIVGIDSAIFMHPSVWKASGHVDAFNDPLIDNRDSKKRYRAD
                     MLIEEHVLKMEEKVAREVEKARERFGTAFDEKKFRETNQCVLATQSKIDDLNRRLAKA
                     MNETDLAELRQIIIDNEIVCPVSGSRNWTDVRQFNLMFSTEMGSTLEGSSRIYLRPET
                     AQGIFVNFHNVQKTGRMKIPFGIAQVGKAFRNEIVARQFIFRMREFEQMEMQFFVRPG
                     TELDWFRHWKDIRMRWHTALGFGEANYRFHDHTKLAHYANAATDIEYRFPFGFREVEG
                     IHSRTDYDLKQHQQYSGKKMQYFDPEKGESYIPFVVETSIGLDRMFLQVIAAAYTEEE
                     LRKEDGSADMRILLRLPACLAPVKLAVMPLVKKDGLPEIAEKIVHTLRFDFNCQYDDK
                     DSIGRRYRRQDAIGTPYCITIDHQTVEDDTVTIRYRDSMEQERVNIRSLKDIISEKVS
                     IGALLKKL"
     gene            23906..25177
                     /locus_tag="PK288_02665"
     CDS             23906..25177
                     /locus_tag="PK288_02665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM17850.1"
                     /translation="MPKVLIITYYWPPGSGAGVQRWLKFSKYLPDYGWEPLILTIDPA
                     YATYPAIDHTLDKEVPSPLTVWKTRATDYFRIYRRDKSRIPSSGFATNPDKEFLDRII
                     RFTRGNFFIPDPRKGWNKYAYRKACELIQEHRISYIITTSPPHSTQLIGLRLRNKYQD
                     IRWVADLRDPWTDIYYYHNFYPTKCAKRIDAKYEKEVLENADKLITVGKSLKELFSRK
                     YPGIEQKTGIVPNGYDEEDFAGITESKPDIFTLSYIGTLSDSYPLNGLLAALSELEKK
                     EAAFRLRFAGFVSENQRVLVSSALGHGKVEFIDYTDHRSAIGYMAASSMLILIIPKHS
                     SSRSIVTGKLFEYMASLKPILCLGPADGDAAEILLESGHKSVFKWDDHTGISDFIGRV
                     MEHPGELAIKKPLSYSRKALTGRLSEILSSL"
     gene            25262..26491
                     /locus_tag="PK288_02670"
     CDS             25262..26491
                     /locus_tag="PK288_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPM17851.1"
                     /translation="MKAGFILNGVFVNDPRVLNEARILAGNGYQVYVLNLPPDKNVPR
                     FVQFEDNIFLVHRRIPKKANNVLFALENTFALFDYSWNISIKRFIKKYGIEILHAHDL
                     YMARAAKRASDHYKIPLVLDLHENYPAAIKEYHWANRFPSRLIVRPSRWEKKEKKYLS
                     YADRIIVLSSSYRNHLLAKYPGTDPVQVKIYPNVPDVKRMSSYAIDRDILPPYDRKII
                     FYFGVISQRRGIMTTIEAAEILLRDNQSIHLLLIGPIDKAEKDRFIRLFAGSEISGHL
                     TYYPWKDIGEFPSYVHASAVCISPIIKNPQHESGVANKVFQYMLFGKPILVSDCEPQA
                     DIVSKTGCGLVFKSGDPVDMAEKLSELINDNEKCIIMGERGKQAVMKEYNTVKQGTEI
                     IKAYRELSASGKKSADT"
     gene            26505..27368
                     /locus_tag="PK288_02675"
     CDS             26505..27368
                     /locus_tag="PK288_02675"
                     /inference="COORDINATES: protein motif:HMM:NF022760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM17852.1"
                     /translation="MEHMHFVITQFNLRNKPVSGSAAYDEWLSWTRSRISIFRNYCLP
                     SVLNQTCRDFSWLIFFDSSTPEEFGEFIGFLSSHDLISVCYSDGMDDFYTGYIREVRK
                     KAGPTTKWVITTRVDNDDSLHRDAILTIKENFIAKHRYLISLASGYVLDVNEKKLSHY
                     FYPMSPFLSLIEDTGKEIEGIYSRGHTKWNNLRLFIFRELWLEYFNPKARVSRFILKK
                     PLWMQTVHGENVSNSFYRGFPVLRKRDLSDFSLELITNRMTFGTITRYCHYVTWKRYM
                     KCLVIKFLINK"
     gene            27390..28424
                     /locus_tag="PK288_02680"
     CDS             27390..28424
                     /locus_tag="PK288_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPM17853.1"
                     /translation="MVIRKLFPKTYNFLVYLINGSRKIFTPYSIYITDSLGGSFKTYL
                     SDPVNINRETELKRGLDAESERTINTVLERIKFYPDEKHRKKVSKSQPVAGGLLPVET
                     AVMKKQIENSLSGDRKKYKRIFRHLDESVFYFYHGLHFLPENVLNYLKNKDLIDIGAH
                     SGDSAIALRKFGFRKTYSVEMSLKSIDQYKKNMAQLKISDNSFELINVCVADADNKEP
                     AKFADTGSTGLSLLRNRGKYDWITVEQKTLDTIASEYRISPGLIKADIEGMSFDFVKG
                     AVGTMKTHRPVLAVAIYHNPHELFEVKPFLEKALDNYTFLIRKLSAGIENNLCHSDIF
                     LIGFPDEAAV"
     gene            28482..29990
                     /locus_tag="PK288_02685"
     CDS             28482..29990
                     /locus_tag="PK288_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPM17854.1"
                     /translation="MQKLIKTSFRDSFIYGLGNISVKVIGFLLIPLYTDPRYFSVDEF
                     GIIALLDISGIVLISLMASGLPQSLMRWYWDDEFRDNQKGIFFMSLSNQVIVSISFCI
                     ILIPLAGRFSTLIFGSEGWSNALKILILSTALQAINNIINSLMRIQSKALLFSVANFI
                     KLAVVLILTVYLIVFRQAGVKGIYIAQVAGNLLFIILLSVYVARNCRIFFNLQVFKSM
                     SRYGFPLLISNFAAASLAVIDRYSLNSLALLKYVAIYTMAYKISSVLKLVIVDSIKMA
                     ITPMVMQKMNSPDNKRFYSKTLLYSSFVLMLGIITLSLFSYEIIKIISRSAEFWIAFI
                     AVPVLSLSVFFANMRETSSYGLIINKKTNIVGISVIASSVLNIILNIILIPVWNITGA
                     AIATITTQFFYWFLNYYYSQKEYHIPYELKKIAIMFICGGILSFAGLMLNELHIVPRL
                     LIKTACVISFPFILYLFNFYEPVELQAIRGFISKWSRLRNLRDNLRSLKNIQ"
     gene            30088..32547
                     /locus_tag="PK288_02690"
     CDS             30088..32547
                     /locus_tag="PK288_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17855.1"
                     /translation="MKINQDLRSALPHLLAVIVFIIISFIYFYPVLEGKVLKANDSTV
                     SSINSKEIRDFREKYGREPLWTNSIFSGMPAYLISTRYPGNLMKHVDTALRIFQTPVS
                     VLILAMAGFYLLLIIFGADPLISAVGAFAYGFSSFLFQILAAGHNTQAIALAYMAPMI
                     GSVYYAYKRNALAGAVLTAVFLSLEILANHPQITYYSMICLIVFIITELIFSIRQKTI
                     ARFLKTSALLLTPLIIAIGINFGNLYTVYEYGKFSSRGKSDLSVSDKNVSKGLNRDYI
                     TYWSYGIDETFNLLVPNFKGGSSKPFDRNSETVKALRQNNASSAAGQLVKYWGTQPGT
                     DGPHYMGAIVIFLFIFGLVIVKGPEKWWLLAASVLSVMLAWGKNFMVFTDLFIDFFPG
                     YNKFRAVTMILVIAQFCIPLLGILALKKIFSVNLSKKEIFRGLKIAAGITGGLLIMFL
                     LLPGIAGSFLNPYESDYPDWLRSALVADRKSLLRADSLRSLILIILSAGTITAFLQNK
                     LRREYSIIIIGLLILADLWSAGKRYLDSDKFERPSVIQKSFTPSVADEMILQDTSYHR
                     VLNLSVSTFNDNTPTSYFHKSIGGYHGAKMKRYQELIDTSITVNIESFIEKARNANTI
                     ADLLPVFDNTSAINMLNAKYVIYNPDAPPLVNRRALGNAWFAGKVKFAANANEELSSI
                     NSLDPSSEAVVDTVFRDYVKSLSFPYNEGDTIYLVSYKPNELIYKCSCSGERFAVFSE
                     IYYPAGWKSFIDGKESPHFRVNYVLRAMIVPEGSHEIRFSFEPDSYFAGNRVSLASSL
                     LLILAIAVYAGAELKKKLKSP"
     gene            32549..33439
                     /locus_tag="PK288_02695"
     CDS             32549..33439
                     /locus_tag="PK288_02695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM17856.1"
                     /translation="MNRPGECPLCGAEKVSYYLTCTDHLVSGETYDLFICNACGFVLT
                     HDPPDEEAMGRYYASEDYVSHSDTKAGVINRLYSLARNIMLSRKLRIISAASGLRKGS
                     ILDIGCGTGYFAAKMKANGWNSTGIEPDCKAREFGTRQFGLSILEPEKISSLPGESCD
                     VITMWHSLEHSHDPFNYMQEILRLLKPEGLFISALPNCGSYDAMHFGDSWAAYDVPRH
                     LWHFTPETFELFAAKTGFKVTHVSTLPFDVFYISLLSEKHRNRKLYLLSGLAKGSFFS
                     VRAVFNYRKSSSLVYHLRRR"
     gene            complement(33531..34508)
                     /locus_tag="PK288_02700"
     CDS             complement(33531..34508)
                     /locus_tag="PK288_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPM17857.1"
                     /translation="MKKLTSIVAAFFFWPLCSGQQLPLYSQYLQNKFLINPAVAGSEG
                     YTNINLTAREQWIGYSGAPRTYSFSGHGRILKTKYSIKADRSGNNRFRPKGDGKVGLG
                     GYVFSDRHGLVQRTGFQAAYSYHMWTGRTTQVSLGLAFTGYHFKINEKEINFEDPNEP
                     WLNNNLRRGIFVPDADFGIYVLNPDFSFGFTSSQLFGAVARIGGEGYRNYSMDRHYYL
                     FGSYNFNADVKTEIQPSVLIMMSEQTKPMADIGVTCNYDQKIWAGVSYRTSKALVANL
                     GVRYTNLFIGYSYDFTLSEIQRLTFGTHELSLAVKFGDSARKYRWLDRY"
     gene            complement(34517..>36874)
                     /locus_tag="PK288_02705"
     CDS             complement(34517..>36874)
                     /locus_tag="PK288_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM17858.1"
                     /translation="DNLGCMNETSFNIANLSAKPRIIPLPKSTGHHVTCPGGNDGVAR
                     IYVREGGTPPFRYWLVYNETETLYSGEFQNIYASDDPSTYLLCTDLKAGSYKLIIRDF
                     NGCELTMDAILTEPPPLIINITPSDYSGSNIDCKGYSTGYAVASVSGGNGGYSYYWYP
                     ESGTLPGNNTGSRLDGIPAGKYYVRVTDLSGCEQIESIVLTDPPGMELAGSQVSRSPD
                     GNYGISCYGADDGFIKIDIAGGSGNYDFLWVGPDGFQSRSKDISQLKSGVYTCTVTDI
                     NGCILMPEPIFTLTQPDKLEVSLQSSVSSHGGFNIDCHGGTGTVETTVSGGSIGNYSY
                     SWTTTNGSGIIDGEKDQLSLSAGSYHLRVSDLNGCNAEKDINLTEPPEIQLKLVPTHI
                     TCQSDTYDNGSVDLTVSGGVAPYNYIWSGGQDTQDIANLTEGVYSVTVTDANGCTKEG
                     SVRINLPPPLTFEKILSAYGNFNISCFGRSDGSIHVNPLSGTPPYSYVWQGPDGFSRE
                     GNEISGLKAGEYVLLITDSNYCTGSETVTLIEPPGELTIDITAFEDIRCNGDKSGEIS
                     IEAVNNAGAVTYLWSDGVLGNSRQGLAAGRYRVIITDSNNCRTDSVIIFTEPEPLMVE
                     LDVTLPTCPDMPDGEIKTRVTGGTDGGYSYLWSDNSTDPDLSNATGGLYSIRVTDMNN
                     CSVEKSLILEAKNPLCLEIPNAISPNGDLVNDVWNIHNKELYPAMEVMIFNRWGEMVW
                     KSERGYPRPWDGRSNGRMLPVDSYHYIIDLNNGTRPVIGHVTIVK"
BASE COUNT        10262 a         8302 c         8254 g        10056 t
ORIGIN      
        1 cctgtccgac acggcaaaat tgtatgcaag tcgtatactg ttggcactga ggcgttccag
       61 gtcggactgt atgtcgaagg gttcatcggg tgccgtacct gtccccaggg taagctgcag
      121 gtaatcagta tcattgaaat atcttctggc atttacatag aatgatgttg taggaccgtt
      181 atccttgaaa taaacataac atttgcccga gaaccagtat ttcctgatat acttctcgac
      241 ggaaaccagt gctatgaata cattcctgtc aaaataatag taatttaatc cggtcgaaac
      301 ggcccatcca gccggcaggg cctgccataa ttccactgcg gccctgtgtg tggggaaata
      361 tgatcccgga ctgtaagcat aatcgagata ggcataattc ttttttgaaa gaaccggata
      421 tgcttcggct tcaaattgcg gttccgtggc atgaataacc ggatcttcac caagtgcaat
      481 atttcctatg tttacgtatg ccgctgctgt accccaattg aacctgtggc ctgctccagc
      541 cctgaacacc tgccagaacc gtgaataagg cactttgaaa taatcgaatg catatccggc
      601 acgcaggtcc gtggaaaccc gtggtttggt ttttgaccac tggctaatgt cccttttaac
      661 ttcaagagca tcagtatttt gaggctcagt catcagtaac gtgtcaataa ccgcctccgc
      721 ttcggcaaat tctttctgcc aggccaggat acgtgccagc aacaccctgg tatctccgta
      781 ggaagggtac tctttcagaa gtttccgtgc ggcactgccg gcttcttcaa gatttccgct
      841 gaatgccatt tcttttatcc tgagatactc ctgctcagga tctgttattt cctgtgaaaa
      901 caggtactgc atacccgcaa agataattac cagtagccac gtaagaatat ttgaataata
      961 tctgcccatt cagcctttct taaaaattga cctaactttc gatatcagct ccgacggatt
     1021 gaatggtttc actatataac cgtcgatccc gagctctccc gcctcacgtt gcatccgggg
     1081 atcgctgaaa gctgtcagga tcaagaccgg ggtgtctttg ccctggtcgg acctgaggta
     1141 tttaaccaac tccaggccgc tgtgaaaagg aagatgaata tcgacaagaa ggaggtcgta
     1201 gtcgtttccc tcaagcaatt ctatggccct gtttccgtct tcagcaacag aggtttcaaa
     1261 gccttctttt tcaagaatca ccgacagggt tttcagtgcc aagggattgt cttcgcaaat
     1321 caatattttc acagcccgga attcttaaat atcagcaaat ttaatcatga aatccccctg
     1381 atcaattttg taaatctcta tgatttagtc aaaaatggtg aattttatca gtgcgtcaaa
     1441 ggaattggga tgaaattttg atttccgcat tattttcaac gaccaacgtt atgtttttat
     1501 gtgacgaata aaaagggggc tgttgaaagc agctcaaatt aaaaggaata ttaattcatg
     1561 ataccataaa tttatgattg aattatggtt tcagcagcag gctgtccttc ttaagtacgg
     1621 tcatgaattg cctgataaag cggtcgggtt cctgtcgaaa ttcggttatt attaacggga
     1681 gattgtcaaa acatcacaac ctcccgttca attttcagaa taacgaatgc gatttcttat
     1741 ttgccatccc atcctgcaag tcttgcccag agccaccagg ccgcgtacgc tttcatattt
     1801 gcattgactg gctgtgaatg ggcggagttg catttgtacc agtcctgacc ctccttatgg
     1861 ctgttttgcc attcaattgc ccagttgccg tcaagggtcc cgtctccgtt ggtatcatag
     1921 gcacatgcat cattgggttt cttattcccg aaatacactc cgtcggggtt atatgattca
     1981 atatccgcaa agtcatatag caccttttta ttattccgac agtagttgcg tatttgttca
     2041 tttctcagat gaagattgcc agtcagccct gtaccgtcaa gatgacccgt catatagaca
     2101 aaggtgacac cgggaaaatc cttttcaagt tcctccataa gctgcaggta tttatttata
     2161 tctgataccg aggctgtgga aacctggccg caccacgacc acatcacaac atttatctcg
     2221 ggatgttcag caagataggt cctagttctt gcagcccagg tggtccggtc gggatttcca
     2281 agatcgccgc tgatggcata atcgtgtata aagagtgcat catcggattt ttttccgctc
     2341 cagctgaagg atgatccctt gaatgaaacc aatcccgaca tgccatcagt tatctggcta
     2401 ccgtgggaag tatggctgta ggcaatgtta agtttccgct ttgcctcgga tatccattgg
     2461 gaagggattg aagcaagtct tatggaatta tggtcggcta caacatatgc gtcaacggcc
     2521 gttggtggta ttggttcatc ttccttgctg caaccaatga atagcaggga tgtaatcaga
     2581 tagaggaaaa ttgccggata attcataaca ttgattttta gtttaatact atttcataaa
     2641 acccatgatt ttcaggaatc attttctcat ggatttaacg aattaattgg attattgttg
     2701 tatgaaaatc agtgtctcaa tctgtatttt attacatcaa tgtgactgac aggaggtttg
     2761 tctgccctga tttccggttc cggaaagtcc ggaatatctg agacccataa ttgacagatc
     2821 ccggattggc ttccggtaat tttatcattt aaaaacttaa tggcttcttt tttaagacgg
     2881 aacctgttat ctcacctgat tctgaagcct gttttccggc catctgtacg gaaaccgggt
     2941 gttcttacac tttttccatt ggtgagcggc aagccttccc tctcccaggc atccattcca
     3001 ccggccatac ttgcgacatt ttcatagccc ttcgaataca tgaaaaggac actttccctg
     3061 ctttttgaac cattgacatc agcaaaaata agcggtttat cgaccggcag atcagggtag
     3121 gatttttgca gcccgctgaa aggcatataa attacttttt ccacattgaa catcttaaag
     3181 ctgttcatgt atgactccct gacatcgaca ataacagctc ctttacggga aagatcataa
     3241 ctctgcctgg gtgacaggtt aattacccca tttgacaagt accctgtttt taaccagtta
     3301 tttctcatgt catatactat atttgctttc tatccggaga ggttctgatt ttaaaatcaa
     3361 gatgaatcct gaagggttca ccgaaggcaa atccgtattt tttaaaaagt attcccatct
     3421 ttatctggcc tgactgagct ccgcggcata attatgccgg acgtcattca tgcgtggttc
     3481 atgttttttt gaccccgtct ttgcgatatc ctttaacagc ttcctggctg ctttcaagtc
     3541 accattgctt atgctatatc ttattctggg aaggtccgga cttgcattga tcaatcctgc
     3601 ttttcttaat tcagcaagat gctgtgatac agttgaatca gccagaggga tagcttttga
     3661 aatctcattg aagcaacacg cttcccttgc tgccagaagc cggattatgg caatgcgggc
     3721 cggatgtgcc agtgccctgg caaatcttgc aagcttcaca tcagccttat caaatagtcc
     3781 ttttttaccc ttgcccatcg accataaatt taagaattta ttgcaataaa tccgattttc
     3841 gtgaaataac gtaagtattc attaatcccg gagaatcaat tactgtcagc agctgacagt
     3901 aattgattca ttattcagat ttttttaatt tctgcagata gtcggaaata caggcatctt
     3961 tatgttttga tttgtattgc attatgaacc gcgggtgttc aaggggttca atatggtcaa
     4021 agaagtgatg tttctgattc agccgtgaaa ggtaacggta attcttaccg gcacctaaac
     4081 aaaagcaaat gttgcgttct attccgaaat ccagctgctt tcgaatggaa gcaactgcaa
     4141 attcttctat tgattcttca agattcctgt tgtcgtaata attgaaattt acctcccttc
     4201 ccttttcatt tctggatgta aatccgagcg ggctcacaga ggttatataa taatcaccgt
     4261 aaaatttccc gggacctcca tatccatcaa tcatttcata catgaagact gacgatatct
     4321 catatgattt tattcccgga atgctcaatc cgcatttctc cttaagtctg acactgtcag
     4381 tgaagggaat tcctgtaagg ccggctccga aacgtcccgg attgatcccg attatcatac
     4441 gcctgggccc cggatcggaa taaaacttcc tgtaaaattc cgatactgcc ctgaacactt
     4501 cagcctctgt aaacggattc atcactgaaa tgccatcagg caggctgccg tcatacttaa
     4561 ggcttccgta gaacaatata gctttgtcgg caaacgttaa catattctgt tgtaacaaac
     4621 aggtgtaaaa ataattattt cactatcatt tagtaattta aacattatcg ttttttttta
     4681 actttggtcg ctttaatttc cagatcaggt gaatgattta ttaagaccgt acggggacaa
     4741 gcagaagacg tttgataaga ggtaccttca gatggctcag atctgggcag gtaattccta
     4801 ttgcatccgc aggcaggtcg gggccctgat tgtcaaaggc aggatgataa tatccgatgg
     4861 ctataacgga acaccctcgg gttttgagaa tgtttgtgaa gatgagaata atgtcacaaa
     4921 accctatgtt cttcatgcag aggcgaatgc aatcacaaaa gttgccaaat caaataattc
     4981 cagcgaggat gccacattgt atgttaccac atccccatgc atggaatgct ccaagcttat
     5041 aatccagtcg ggaataaaaa gggttgtata ctgcaacaga tatcataaca ctgatggtct
     5101 tgatcttctg aagcgtgccg ggatagagct ggtttatatt gattctgaaa atgatattta
     5161 tgaagtacaa taataccaac aggagtattt atttacccgt gctgtttgca gttaccctga
     5221 ttgcgggaat acttacaggc atttattttc ccggcaggaa caggaaccag gtacaaccag
     5281 tcctgagggc cagacatgac aaaataaatt ctgttttaaa tatcattgaa gctgattatg
     5341 ttgattcaat aaaccgcaat gatctggttg aggctgccat ccccgccata ctgaaaaaac
     5401 ttgatcctca ttcagtatat attcctgcca aggatcttgc gcgtgccaac gagcccctgc
     5461 agggcaattt tgacggaatt gggatatcct ttaatctcct gacagatacc atattgataa
     5521 taagtaccat acctggcggt ccctccgaaa agatcggtct gcttccgggt gacaagatca
     5581 tttatgtaaa tgattctctt gtggcgggaa aacagatctc ggaagagagg gtgatgggaa
     5641 tgctcaaggg gccacgtggt acaattgtca atataaaagt acttcgcaag ggagtgggtg
     5701 atcttcttcc atttgagatc aggcgcgaca aaattccgat aaacagtgtc gaagtaagct
     5761 acatgcttaa ctccaataca ggatatatta agattataaa tttcgcgatt accacatttg
     5821 atgaattcca aaggagcatg aatgaactca ggaaggaagg catgacaagg cttatcctcg
     5881 atctgaggca gaattccggg ggggtcatgg agtctgcaat aagaattgct gaccagtttc
     5941 tcgaaaaagg acagctgatt gtatttacca aaggccggac acaacctaaa aatgaagcca
     6001 gggcttccgg aaaaggggaa ttcagatcgg gtgatcttat tgtgcttatt gatgagtgga
     6061 gtgcctctgc aagtgagata cttgccggag cagtccagga taacgatcgc ggaacgatta
     6121 tcggaagacg ctctttcggc aagggacttg tgcaggagcc agtctccttt gccgatggtt
     6181 ccggaataag gttgacgatc gcacgttatt acacacctac agggaggtcg atccagaaac
     6241 cctacaataa tggttttgat gattatttcg acgatctgaa ttcaagattc aggcatgggg
     6301 aatttgagat ccctgacagt atacattttg ccgactcact gaaattcatc acacccggtg
     6361 gcagagttgt ctatggcgga ggcggaataa tgcctgatgt atttgttccg cttgatacta
     6421 gcggaatttc cccgtatttc ctggcagtca ggccccagat ttatcgcttc gccctgaaat
     6481 acactgagaa taacagggaa aaactgcaaa aattcactga aaccactgac ctggagaaat
     6541 atctgaacag ccagcgcctg atggaacagt ttactgaatt tgtagccggc accggtgtga
     6601 aaaaagatcc ggaaggattg aaaatctccg gattgctgat tcatacccag cttaaagcat
     6661 acattgcaag aaacatactc gacaataagg gattttatcc tatcctggat gatatagaca
     6721 acactcttca ggttgcaatt aaatatatgg agaagaacaa ataagcttct ttctcaatat
     6781 ttttattcat gtccctgccc cggcggaaag catttttaac cgttttggag atccctgatg
     6841 aattctgtaa ctatctgaaa ggattgatcc tcctcctcga tgaatccgag atgtccagaa
     6901 ttttcaagta tcgctattct ggcatttgca ggaagcttta cccgtttatg tatttgatcg
     6961 aaaggtatgt agttatcatg cctgcccaga atccaaagac agccggcact gcccttttcc
     7021 attaccgata cacggtccgg cctgatcatc atcccgttga gtgcagcaat aataccctca
     7081 tcccttatcc ttgaagcaat attcttggaa ttctgcaggg cttcccggaa tttttccata
     7141 ttcatcgtgg caaacatccg tgaaatattt tcaggataca tcagatattt ctttcccgat
     7201 tgcacaattt ttatttcgtt ttctcttttc tggattgttt caggtgtgtc agcaaatggg
     7261 tgcgaatgaa acagacagta tcctgtaagc agttccggaa acagttcaag aaaggcaagt
     7321 gtaacgtacc cgcccatgga atgaccaata agaaatgcct tccttattcc caggctgtcg
     7381 agaaggctct ttaccgcttc agccatgaac tcggttgtgt ggcaatcacc ataaacggcc
     7441 gaacggccat gacccggaag atccaccgaa attactcgat gtaaggcaga aagcctgccg
     7501 gcaaaccttt cccagatctc tgatgactcg agatgaccat gaatcaaaat taccgggctt
     7561 cccatcccgg aatcggagta atatatctta cctccgttat gaaggatgta gtatttcatt
     7621 gtatgctgta tattaaatga ttaaacggat tactcaaaaa aagaaaaatt atgccgggca
     7681 ccatactaat atagtgaatt gtaacaattg tatatctctt ttaattggat atctttacgc
     7741 ctgattattt tcagtatcct gaaaaaaata ccagcccaat gaacaaagtc ttgttctcta
     7801 caatctccaa aaaattcatt atggcactgg caggactgtt cctgcttatg ttccttccgg
     7861 tgcatctggc cgttaacctg atgcttctga aacccgatcc ggaaccgttc aataaggctg
     7921 cccggttcat ggctactttt cccttgatca gaatcatgga aatagtgctc tttgcgggaa
     7981 ttgttattca tatcacaacc ggcctgatca tacagattca gaactggctc gcacgtccgg
     8041 ttggctatgc aggcgggagc aaatcagaaa cctcattttt ttcaagatac atgatctgga
     8101 caggagcatc cataatgatt ttccttgtcc ttcacttttt caatttctac tttatcagac
     8161 tgggcctggt tcccggaaat cctgatgatt tttacacagt tgcccacaac cttttcagta
     8221 tccctgcata caactatatt tatctcagct gctttttact gctgggcttg catctttacc
     8281 atgcattctc ctcagctttt cagacactgg gtctcaatca caggatctgg acccccgtta
     8341 tcaggatcat ttcactgatc tattccattg cgattcctct cggatttggc attatttcca
     8401 tcattatctg gaaattcaga tagaagcaca tattcttatg aacacacttg attccaaaat
     8461 acccccgggg ccgcttgccc agaaatggac gaggttcaaa aatgaggtca ggcttgtcag
     8521 tcctgccaac aaaaggaaac ttgaaatcat tgttgttggc accgggatat ccggtgcagg
     8581 agctgcatct gcccttggag aactggggta ccgtgtaaag aatttctgtt accaggatag
     8641 cccccggcgg gcgcattcgg tagctgcgca gggcgggatc aatgctgcaa agaattacca
     8701 gaatgacggc gacagtgtgt accgtctttt ctatgatatg ataaaaggtg gcgatttcag
     8761 ggcccgggag gcgaatgtat acagggcggc agaagtgagc aaccaggtta ttgaccattt
     8821 cacggcactg ggggtaccct ttgcacgtga ttacggaggc acactcgaca cccgttcatt
     8881 cggcggtgtc caggtgtcac ggactttcta ctccaaagga cagaccggac agcaatgcct
     8941 gcttggatgt tattctgcgc ttaacaggca gatacaaaaa ggaaatgttc agatcttccc
     9001 caggagggag atgcttgatc tggtggtggt cgacgggaag gccaggggca ttatcacacg
     9061 gaacctcata acgggtgaaa ttgaacgtca ttcggcaaat gcagttgtac ttgcaacagg
     9121 aggttacgga acagtctatt atttgtcaac ccttgctgta aattcaaacg cgtcggcagc
     9181 atggaaagct cataaaaagg gtgcattttt cgcaaatcca agctttctcc agatccatcc
     9241 tacaagcgta ccccagctga accaatacca gtcaaaactg acactcatgt cggaatccct
     9301 gaggaatgac gggcgattgt gggttccctt gcagaaaggc gacaaacgcc ccccgaacgg
     9361 gattcccgag gaagagagag actattttct tgaacgcagg tatcctgctt tcgggaacct
     9421 tgtcccgcgc gacgttgctt cacgggccgt caaggaaaga tgcgatgccg gttacggggt
     9481 gagtgaaacc ggactggcag tttatctcga tttccgggat gcattgaaaa aacaggggag
     9541 gaaagcgatt gagaacaaat atggcaacct cttccagatg tataagacca taacaggcat
     9601 tgatccttac gaggagccga tgaggatcta tccggccggt cattacacaa tgggcggtct
     9661 gtgggttgat tatgagctta tgaccaccat cccgggtttg tatgcaatcg gggaagccaa
     9721 tttttcagat cacggagcca accggcttgg tgccagctct ctgatgcagg cagcaggcga
     9781 cggcttcttc atcctcccgg ttacaatcag caattacctg gcaggagaga tccggtcagc
     9841 accggtgccc ctgtcccatc ctgaattcga ggcagctgaa aaaaccgcag ttgccaggct
     9901 taacaggctt atatcaataa aaggcaacca gactgccgga tcctttcacc gccgacttgg
     9961 aaaaattatg tgggatcatt gcggaatgac acgcaatgaa gagggcctga aaaaggcact
    10021 tgtactgatc agggaactga gggacgaatt ctggcaaaac ctcaacgtcc ccggtacaat
    10081 ttatgaactc aaccaggagc ttgaaaaggc aggtcgcgtt gctgattatt ttgagcttgc
    10141 agaattactt gtgaatgatg ctcttgaaag aaaagaatca tgcggtgccc atttcaggga
    10201 agagtatcag acccccgacg gggaagcccg gcgcaatgat gccgaatatg catatgtggc
    10261 agcctgggaa tatacggggg aaaacactcc gcatgttctt caccgggaag aacttaaatt
    10321 tgaagaggtt gaactcaggg aaagaagcta caaataatgt atcaggcagg tatcaggcat
    10381 aaattaaccc cgggcggtct ggtcaatgtt ggcggaaatg actattaact tgaaaaagga
    10441 acaaaaaaga gatcatatga agcttacttt aaagatatgg cggcagaagg atgcgagggc
    10501 taaaggcaga tttgtaacat atgaactgga tgatgtatcc gagaagatgg cattcctgga
    10561 gatgcttgat tcattgaaca ggaaactcgt ggaatcagat caggaaccgg ttgctttcga
    10621 tcacgattgc agggaaggga tttgcggttc atgtggaata tacatcaacg gtcatccgca
    10681 cggacctgtt ccagcaataa ccacatgcca cctgtcgatg agatatttta acgatggcga
    10741 taccgtctgg atagaacctt ggcgggctaa atccttcccg gtaatcaggg atctgatagt
    10801 ggacaggagt gcctttgaca agatccaggc agcaggcggc tatatatcag taaacaccgg
    10861 gtcggctccg gaggccaact caataccaat acccaggaaa aattacgaca atgcctttga
    10921 tgcggcaatc tgcataggct gtggtgcctg tgttgccgca tgcaagaacg catcagccgt
    10981 tctgtttgtt tcggcaaaaa tcacccagct tgccctgctg ccgcagggcc gggttgaggc
    11041 ccgggacagg gtgcgggcca tggtcgaaaa gatggatgag cttggattcg ggaactgttc
    11101 aaacaccgga gcctgcgagg cagaatgtcc caaagaaatt aaaataaagt caattgcggc
    11161 aatgaaaagg gagtattaca aagctttctg aaatcaattg ccccgagcca cttgccatga
    11221 acaggaaaag atttctcgga attgccgccg catcaggcgc cgggttgtgg attaacagcg
    11281 ggaactttct gaaaaacgga cgcttgtacg cctcatctcc taaaccatcg gtcgcaaaaa
    11341 ataatgaact tggcgccgat ctcataatag cagggggagg cctgggaggt tgtgcggctg
    11401 cactgtcagc actgcgtaac ggactgacgg tgataatgac cgaagaaact gactggatcg
    11461 gcggtcagct gacccagcag ggagttcctc ccgatgagca ccaatggatt gaaacccaag
    11521 gttcaacgaa gttgtatcgc gatttccgaa acatgatacg cagctactac ttcaggaatt
    11581 acccgctgac ggaaaaagca tctgccaaca aattcctgaa tccgggcgac ggcgcggttt
    11641 caaggctgtg ccatgagccc cgtgcagccc ttgcggtact gaatgaactt cttgccccat
    11701 ggataagcac cggccgcctc atcctcatca ccaactgcaa gatcaccgcg gcggatgtct
    11761 caggaaatat tgtcagatca ttacaggcat caatgggacc aggtggcaga aacatcattc
    11821 tttcaggccg ttttttcatt gatgcaaccg aacttggcga tcttcttccc ctgacaggaa
    11881 ctgaattcgt aacgggaact gaatcgcgga aggatacccg cgaactccat gcacctgaga
    11941 agggggatcc ggacaatcaa caggccttta cctattgttt tgccatcgac catattgaag
    12001 gtgaaaacca caccattgac aaacctgaag cctatgattt ctggaagaac catgtgcctg
    12061 agctgactcc gccctggtca ggtaaattac ttgatctccg gtattccaat ccttcaaccc
    12121 ttgaaccgaa aaaactcgga ttcaatcctg acggatcagg tgaaggttca atactgaatc
    12181 tctggaatta caggcggatt atcaacaggc ttaacttcag gccaggagca tatgccagcg
    12241 acattaccct tgtaaactgg ccgcagaatg attatatgct tggcaatctc ataggagtcg
    12301 gcactgctga acacgaaaaa catttatgga atgcgaagca gttaagtctt tcactgctgt
    12361 actggctgca aaccgaagct ccgcggcccg gcaagggaac aggatggccg gggctgagac
    12421 tccggaagga tattatggga accgacgacg gtctggccaa atatccctat gtccgtgaat
    12481 caagaaggat caggggagtg tttacgatat tagaggaaca tgtgggaaaa gaaaaccggg
    12541 gtctgataac aggcaacaaa tcggaagtca gggctgccgg attttttgat tctgtcggaa
    12601 cagggtatta tcacattgat cttcatccct caaccggcgg caacaattat attgattttc
    12661 catcactgcc gttccagatc ccgctgggtg ctcttctgcc cgtaagaatg gataacctga
    12721 taccagcctg caaaaacatt gcgacaacac atattaccaa cggctgctac cggcttcatc
    12781 cggtggaatg gaccatagga gaatcagcgg gcatgcttgc cgcgtttacc ctcgcaaaaa
    12841 aggtccggcc aagggaggta cgggagaaag gacagctgct tgaagaattt caggagctga
    12901 taaggtccca aggaatagaa acacactggg aggaatgaat ctgtcaattg ctaatccaaa
    12961 cacttaatac agaaacaatt catgaaaagc attcagacac gacgtgattt tctcggaaaa
    13021 tcaacacttg caggaatggc ggcactttca gtcccgctaa catcctgttc cgatactgac
    13081 agaccggtag aagaaaaagt agcccggccc ccggaatttt tagtaccagc cggtgatgct
    13141 attccgataa caggcacttt tctcgatgag atatcgcatg acattccaca tcagaactgg
    13201 ggcgaaaagg aatgggatgc tgattttctg aacatgaaag ccattggtat tgatactgtg
    13261 ataatgatca ggtcgggata caggaaattc ataacctatc cgtcaaaata tcttcttgcc
    13321 aaaggttgct atatgccatc ggtcgacttg ctggatatgt ttttgcgtct tgccgacaag
    13381 cacggaatga atttctattt cgggctctac gattcaggga aatattggga cacaggagat
    13441 ctttcatggg aaattgaaga caacaaatat gtgattgatg aggtgtggaa ggattatggg
    13501 gagaaataca aaagcttcag gggatggtac attagcgggg agataagccg aaagaccaag
    13561 ggtgccattg gtgcatttca tgccctcgga aagcaatgca aggatgtgtc gggaggcctt
    13621 ccgactttta tctcgccatg gattgacggg aaaaaagcgg tggaagcagc atcgggtgac
    13681 ataaccaggg caaaagcagt ctccgttatt gaacatgaaa gggaatgggg cgagattttt
    13741 gacgggatcc atgatgttgt tgacgcctgt gctttccagg acggtcatat tgattatgac
    13801 gaacttgatg cttttttttc agttaacaag aaactggctg acaaatacgg gatgaaatgc
    13861 tggacgaatg cagaaacatt tgaccgtgac atgcccatca ggttctttcc catcaagttc
    13921 gacaagcttc gccttaaact ggaagctgcc aaacgtgccg gttatgagaa agggatcaca
    13981 ttcgaattct cgcatttcat gagccctcag tcagcttatc tgcaggccgg ccatctatac
    14041 aggcgataca aggaatattt tggtattgcc tgagaatgat cccggcccgg atccaatgct
    14101 gttcaccagg aacagagcgt ctcgatttcc ttaacagttt ttggaacggc ctttcccaga
    14161 accctgttgc ccttttcggt aataaggatg ttatcctcga tccggatacc cccgaaatcc
    14221 ttccagggct ccagctttga ataattaatg aattccttgt gtcttccctc tgctttccat
    14281 ctgtcaatca gctggggtat aaaatagcag ccaggttcta ccgtcagaac atgcccgttc
    14341 ttaaaaggca gtgcaaaccg tagaaatgcc agtccgaact gactgcttct ttttacgtca
    14401 tcattgtatc ctacgaagtc ctcaccgagg ctttccatat catgtacatc aagtcccatc
    14461 aggtgcccca gcccgtgagg catgaaaagc gcatgggccc ctgcttcaac ggcctcatca
    14521 acatcacctt tcatcagacc aagatccttt agggaagaag ctattatcct gcatgcgttc
    14581 agatgcagtt ccaggttcga catgtcgggc ccagcggccc tgatggattc ggtatttgcc
    14641 ttcaggacaa cctcgtagat atcccgctgc ttctggctga atttccctcc gacaggtgtg
    14701 gttcgggtaa tatctgaagc ataatgcatg cctgtttctg ccccggcatc ggtcaccatc
    14761 atccttcctt ttctcaaaat gttgccatgg tcgtgattat ggagggtctg cccgttaacg
    14821 ctcaggatta cgggaaaagc cggacccgca ccttttgccc atgcaattcc ttctatgata
    14881 cctgctattt cctgttcttt aatacccggc ctgcacattt tcatggccgt ggtatgcatt
    14941 tcgaaggtta tagccaatgc ctgttcaatc tcttcaatct caaccttctc ctttattgaa
    15001 cgcatcatga ccactgccct gattagtttt tctgatgccg atgccttcat ctggcaggga
    15061 ttctccttta ataatgatcc cagtgtcatc ctggtttcgg aacggtacgg tggaagaaaa
    15121 tggatcggac gcttttttga aatcgcttcc ctgataacag cttccagttt tgaaagtgga
    15181 ccagtgaaat tagttccggc agtttcagca agttccctta ctgaaggctg agaacccatc
    15241 cagataatat cgtccatatc aaaatcgttg ccgaatatcc agtcttttcc cgtatcaaaa
    15301 tccatcaccc ctgcaagatc cggaagatca agcccgaaaa agtagaggaa atcactgtcc
    15361 tgcctgaaat gatacggatt tgccgggtag ttcatcgggg aatcagtgtt tcccagaaac
    15421 agcgccagcc ctgatctcat cagaccatgg agccgtgctc tcctttttat ataaacctcg
    15481 cgcctgaaca tatttttaca gaattatatt aatattcccg actcctaagt taacataccc
    15541 tcgcttaagt gcaagccaaa cctggaaaaa gaaaatgtca ggcataattt gaatcaaaag
    15601 gctgcgggcc acttcaaggc aggtcaaata ccgtatttgg tggctaggtt ccctatcctt
    15661 gcaacaaaat catccccgcc tttttctgca taacggcttt ttgcaaccga gttgaatttt
    15721 gataaaatta tactgtcatc atcttccgac ataacctggt cagccagcct gaataacaca
    15781 ttgttttcct ttgaaatatg acttctcagc agctcactgt accccttcat gttacgagca
    15841 atatcccccg ctgcggcttt attgcctttc ctgtaaagtt caagtccttc tgagatccct
    15901 ttcacaaaat tacggcctgc ggcatgctca tggagcatta ctgcaacagg gcccgactgt
    15961 gaggaaaagc cccttgtttc aaggaacgga aacaatattt cttcttcctt gacatgatga
    16021 agaccatcgg caaaattcct gatcagatac acaacctctt caatatggga agcatctggt
    16081 tcatcctgcc cggcaataat ctccataaca tctatgagcc tcaaaatatg aacatgatcg
    16141 ttttccagat ttccagttga atttttcatt tttcgatatt ttttacacgc aaacttatca
    16201 aaatatatat aaagagtaag cgatcttttg gtattttgtc agcttatttt tttacatttg
    16261 tctgcaatca caccattaaa ccgatataca atgtttaaca gggaaacgga atatgccctc
    16321 aggagcctgg tatatattaa gcttcagaac agtaagaaca ggaggcccgg ggcagttgaa
    16381 atagccaggg aaaccggtgc tcctcatttt tatattgcca aaatcctcca gcgtcttgca
    16441 agggagggat ttattgagtc ggtgaaaggg aagggaggcg gattttattt tgatccgggg
    16501 aaacccgatc tcacattaaa agagctgata acttttattg aaggtgacag gtctttttca
    16561 ggatgcggtt tcgggttaaa gcaatgtgat gagaacaatc cctgtccgtt tcatgagaag
    16621 tatgcaccga tcaggagatc acttgataat ctgctttctg aagagacagt tcaaagcctt
    16681 gcaaatggtg tcaggctctg aaatctttaa tgacaaaatg aaatgataaa aggctggtca
    16741 aaattgcccc tcgttgtttt tttgtcaaca gccggtttgc ttacgctggt tcaatgcatg
    16801 gtgaaaaatc caatgctttt ggcagagcgg tttctgaagg gagccggatg gattgagata
    16861 gctgccattt caatttacgg ggcatttgtt gcaaaccgga tggaagatcc gtcacgggcg
    16921 cgcagatgga gggtcataac atggacgact ttttcaacag ttttcttttt acagctgctg
    16981 atcgggattt ccggtcatga aaagttttta atgaccggga agcttcatct ccctgtaccc
    17041 atgatgataa taggagggcc cgttttcagg ggacagatat cattcatgac catcctgttc
    17101 cttagtacca taattctttc aggaccggcc tggtgcagtc agctatgcta tttcggtgct
    17161 ttcgacaacc tcgcatcaat cggaagaaca actaaaaaac cacttcgcaa caaagcagca
    17221 ctgaaatcca cctttcttat ccttgttatc accgctgcat tgatattgag atggctcaat
    17281 gtatccatag ccattactac cataatcgca gcttcatttg gtattgcagg acttgcagta
    17341 atggtttttt tctccaggcg gaggaaaaag atggtgcatt gcatccttta ctgtccggcc
    17401 ggtttaatgg tcaacctgct gcggcacatt aatccgttca ggatgtatat cgatcagagc
    17461 tgcacccact gcatgctatg cattaaatat tgcaaatatg atgcattgaa tgaatctgac
    17521 ataaacaata aaaggcccgg cttcacctgc acgatgtgcg gagattgtct tgctggatgc
    17581 aggcacaact cgatcaaata caaattcctc agcctgaaac cggagaatgc caggaacctt
    17641 tacataattg taacaattac ccttcattcc gtttttcttg ccctcgcaag gatctgaatt
    17701 ctgttgatca cctgtatccc ggcatcattt cagcggaaaa aagatattaa caaatatctt
    17761 acttaaattt ttacagaact ttttaatttt caatttattt tatttgcgaa taatattgat
    17821 tgttaataaa tcgttattat attcgtaaga aagttgtcaa cacattatta acaccagcct
    17881 gccggtatgc cttacagaag attaccgaac actgatgcgg cacgtatcag ggcgatgaag
    17941 attgcgcttg aaaaaggcag aaatgttcat cccaagcaaa tgcctttctc agggaaactg
    18001 atggtacgac ttcacaagtt tctcccactt ttcgaaaata tgatacagct tcagcggcag
    18061 tcatacgctg cccaatataa taaaagccgt gactattccg agattgtaag aaaagcaagg
    18121 ctttacctga ctcattttgt taaggtgatg aacatggcga tttacagggg ggaaatatca
    18181 cctgagatca ggtcctatta cggcctggca acaaatgaat cgggtgtgcc atccctgaac
    18241 accgaaaatg aattgataag ctggggcaag aggatcattg aaggagaaga gtacaggctc
    18301 cgcaagggtg gaagtccgat aacgaatccc acaattgccg tggtgaaggt aagataccag
    18361 aatttccttg atgcttggac atttcagaaa accctggcta aaaggaccat ggattatgtc
    18421 gagaaaaaca gtgttctgag aaaggaagct gacgaaatta ttcagcagct ctggaatgaa
    18481 attgaagaat cgcacagtat attacctgat gataaacgaa ttcctgccaa cgaggaatat
    18541 ggcgttgtgt atttctacag gaaaaatgaa ctcaatgata tccctgccga atcaggatcc
    18601 ggggataagg tctggaaata agtagcacca tttgaaaata gggaatataa cactggcaga
    18661 aaggcccttg tttctcgcac cgatggagga tgtgactgat ccctcattca ggctggtttg
    18721 taaaaagttc ggagctgatt tcgtgtacac tgagttcatc tcatctgatg gtttgataag
    18781 ggacggggag aaaagtgtca aaaaacttga gatttatgat catgaaaggc ccatcgggat
    18841 ccagttatac ggtcaccttc caggggcaat ggttgaggct gccgtcattg ccgaagaagc
    18901 cgggccggag cttattgaca ttaatttcgg ttgtcccgta aaaaagatcg ccaaccgtgg
    18961 agccggtgca gggatgctta aaaatatccc gctcatggtt gaaatgacac ggatgatagt
    19021 tgattccgtc aggcttccgg tgacggttaa aacaaggacc ggctgggacg attcttccag
    19081 gaatattgtt gaggtttccg agatgttaca ggatgccggc ataaaggcaa ttacaattca
    19141 cggacggaca agggcgcaaa tgtaccgggg tacggcagac tggagcataa ttggcgaggt
    19201 taagaaaaac ccaaggctga ggataccggt tatcgggaac ggtgatatta accatcccgt
    19261 aaaggcaaaa gagatgtttg accgttacgg tgttgatggg atcatgatag gccggggagc
    19321 tgtcggcagg ccctggatct tcagggatat caggcacttt ctcgataagg gcgaactgct
    19381 tcctgagcca acggtaagtg aaaaggccgt actggcgttg ttccatttcg aaaaatccct
    19441 ggaattcaag tcaggcaaaa gagccattct tgaaatgcgc cgtcacctgt caaactattt
    19501 caaaggtttg ccaggtttca gggagacaag gcttaaactg ctgacctcgc ttgaaccatc
    19561 tgagataaag gatcttatca gggatatcgg ggaaaaatgg ggcgattacc gcactgatga
    19621 cagaacatcc gtatacggtg agtgagtgcc aggttccggt ttctcaaccc tgcctgaaag
    19681 atccgaaaag ctgccccatc tgaataaaat cctgaggatc acaagaaaca ctatcccgga
    19741 aatgagcaaa gctgaaaatt ccgcccacgg ataaatattg gactgccgga atacagcctc
    19801 cgtctgaagt gatgatgaat cgtgggtcaa cattatgcaa aggaagatat tatttgctgc
    19861 atgtgctccg attgctgcct caattccgtc atccataatc gtgacgatcc cgaaaataat
    19921 gccgaacatg aaatactggg gcatcattgt catgaagcca aatttctcca cctcgggatt
    19981 gaatgaatgt aaaagaccga agagcagtga ggtcataacc agtgggaccc atttgttacc
    20041 ggcaagcact gtaaatccct gcatcaggta tcccctgaaa aggacttctt caaaagcagc
    20101 ctggaacgga acaagtacca gggaaataat gatcaggaaa aacaatgtgc gggatgtatt
    20161 gttgagggtg aagttcgaag gatccatttt caggtaaaca accaggtaaa cagctgagag
    20221 gatgaaccac acaaggcccg acacgaagaa tctcttccac ctgaaattgc ggtttccgtt
    20281 tattgtcatg gcaagggtcc ttccgttcaa tggctttacc agcagggtaa atgcaatcag
    20341 gccggcaaca aaagggaaaa gcatcaggat caggcccaga ttcggatcca ggcccaggat
    20401 gctcagatct cccgggtgtt ctgcaattcc gctgatcacg cctgtgtccg aggcggccct
    20461 gagcgcatat gcaatcagaa gcgggatagc cccaagcgta ttggatatga tcaatactgc
    20521 tgcgaacatt acgatatatc gccagaagct gtttttgcct gcaaaagatg atcggagatg
    20581 aatcatggtc agtttggtta tattccgcgg tcagtctttt tgggataatc tatcgaataa
    20641 tgaagaccaa tgctttcctt ccggttcata gccattttta ttataagata gcccacatta
    20701 atgagatttc taagctcgca tatctttttg gagatcagag actttttata caggctctca
    20761 gtttccctgt agaggatctc aaggcgcacc atggccctgt taagccggag atcggacctg
    20821 acaattccga cgtaattgct cattatcgtc tgcacctcct taaggctctg ggtgataagc
    20881 accatttcct caagatgggt tgtaccctta taatcccatt caggaataga atcttcgaaa
    20941 tcaagttcgc caatcctttt cgcagagctc atcacagccc ggtgggaaaa gaccacagcc
    21001 tcaataaggg aattggaagc cagacggttc gcaccatgaa gaccggtcga agaagcttca
    21061 ccaagagcat aaagcctgtc tattgagctc tgtccgtcaa tgtccacctt aatcccgccg
    21121 cacgaataat gaacacaggg aaccaccggg atcatatctc tggttatgtc tatacctctt
    21181 tgaaggcaat gttcataaac attcggaaaa tggtttctga ggccttcagg atccaggtgg
    21241 gtgcaatcga gccagacatg gtcctctccc cagatcttaa gttcattgtc aatggcccgt
    21301 gcaacaatat ccctcggtgc aagcgatccc ctgctgtcgt acctgttcat gaacttttcc
    21361 cctgcaatgt ttttaagaat agcaccatac cctctaaggg cttccgtgat cagaaaagag
    21421 ggtttttttt caggatcata aagggaagtg ggatgaaact ggatgaattc catattttcc
    21481 actacaccct tggccctata taccatggca acaccatcac ctgtagctat ttccgggttt
    21541 gtggtggtct gatacacgtt ccccagtccg cctgttgcaa tcacggtgat ccttgacaga
    21601 aaggtgtgaa ccctttggcc gacaagatca gcaacatatg ctccgtaaca tttaactccg
    21661 gcagaatttt tcttgatctc gatccccagg tgatgctggg tcaggatctc gattgcgaaa
    21721 tgattctcaa atatttcaat gttgggatct ctccttacct tgtcaatgag tgtctcttca
    21781 atagcctcac ctgttttatc ctttgcatgg aatatccggg gctcggtgtg cccgccttcc
    21841 ctggccagat cgtaagtgcc atcctccttt ttgtcgaagg ggaccccgaa atcaatcaga
    21901 tcctttatcc tttcgggagc ttcctgcacc acaagcctta ccacttcttc attgcagaga
    21961 ccgtcaccgg caatcagggt atccctgatg tgtttctcaa aattgtcggg ttcgctgaaa
    22021 acagcagcaa tcccaccctg ggcatacctt gtatttgtat catctaaaac ggatttggtt
    22081 atcaccgata ctttcccgta ttgagctgct tttatggcaa acgtcagccc ggcaattcct
    22141 gacccgatta cgagaaaatc cgtctttttc cgcatgactg ataaatttgg acagataaag
    22201 atacaaattt atgtttttac tgttttgaac cgttatttcc gccaggttta ataattccgg
    22261 cgcacggaac agtgccgtat tgcccgaaca attattgtca aatcaaacat ttcagttatt
    22321 tttgctcacc tttagacaat aacatctcag tttatggcac aggaggatgt tttcaggaag
    22381 ctggttgccc attgcaagga gtatggttat gttttccctt caagtgaaat ttatgatggc
    22441 ctgagtgccg tttatgacta tggtcagtac ggcgtagaac ttaaaaacaa tatcaggaaa
    22501 tactggtggg acagcatggt cctcctgaac gacaacatcg tggggattga ctcggccatt
    22561 tttatgcatc cgtctgtctg gaaggcatcc ggccatgtgg atgctttcaa tgatcctctt
    22621 atcgacaaca gggattccaa aaaaagatac agggccgaca tgcttatcga agagcatgtg
    22681 ctgaagatgg aagagaaggt agccagagaa gttgaaaaag caagggagag gtttggtacc
    22741 gcattcgatg aaaagaagtt cagggagacc aatcagtgtg tacttgcaac ccagtcgaaa
    22801 atagatgacc tgaacaggcg cctggcgaag gcgatgaatg aaactgacct ggcagagctc
    22861 aggcagatca ttattgataa tgaaattgta tgtcccgtat cgggaagcag gaactggacc
    22921 gacgtgaggc agttcaacct tatgttttca accgaaatgg gatcaactct ggaaggctcc
    22981 agcaggattt atttacggcc cgaaacagcc cagggaatct ttgtgaattt tcataatgtt
    23041 cagaaaaccg ggagaatgaa gattcctttc ggaatagcac aggtaggcaa agcattcagg
    23101 aatgaaatag ttgccaggca gtttattttc aggatgcggg aatttgagca gatggaaatg
    23161 cagttttttg tccgccccgg taccgagctg gactggttcc ggcactggaa ggatataaga
    23221 atgagatggc acaccgcatt ggggtttgga gaagcaaatt acaggtttca cgaccatacg
    23281 aagctcgccc attatgctaa tgcagcaacc gatatagaat acaggttccc tttcgggttc
    23341 agggaggttg aaggaataca ttcaaggacg gattatgatc tgaagcagca ccagcagtac
    23401 agcggaaaga aaatgcagta tttcgatccg gaaaaggggg aatcatacat tccttttgtg
    23461 gttgaaactt caatcggcct tgacaggatg ttcctccagg tgattgcagc cgcatatacc
    23521 gaagaagaac tcaggaagga ggatggttca gccgacatgc ggatcctcct caggctacct
    23581 gcctgtcttg ctccggtaaa gcttgcagta atgccgctgg taaaaaagga cggactgccg
    23641 gaaattgcgg aaaagatcgt tcacacacta aggtttgatt tcaactgcca gtatgatgac
    23701 aaggattcga taggcaggcg ctaccgaagg caggatgcca taggcacacc ctattgtatc
    23761 actatcgatc atcagactgt tgaagatgat actgttacta tcaggtaccg cgattcgatg
    23821 gaacaggagc gggtaaacat ccgaagcctg aaagacatta tctctgaaaa ggtgagtatc
    23881 ggagctttgc ttaagaagct gtgaaatgcc aaaagttctc ataataacct attactggcc
    23941 tcccggttcg ggtgccggcg ttcagagatg gctgaaattt tcaaagtatc tgcccgatta
    24001 cggatgggaa ccactcattc ttacaattga tcccgcttat gccacttatc ctgccattga
    24061 ccacacgctg gataaagagg ttccttctcc actcactgtc tggaaaacca gggcaaccga
    24121 ttatttcagg atttaccggc gcgacaaatc aaggatccct tcctccggtt ttgcaacgaa
    24181 ccccgataag gaatttcttg acaggataat aagattcacc aggggcaact tttttatccc
    24241 tgatccaagg aaaggctgga acaaatacgc ttacagaaaa gcctgtgaac tgatccagga
    24301 gcatcggatc agttatatta ttacaacaag ccctccccat tcaacccagc ttatcggcct
    24361 gagactcaga aataaatatc aggatatcag atgggtagcc gacttacgtg atccctggac
    24421 cgacatctat tactatcaca acttttatcc taccaaatgt gcaaaaagga tcgatgcaaa
    24481 atatgaaaag gaagttctgg aaaacgctga caagctaata acagtaggga aatcccttaa
    24541 ggaacttttc tcacgcaagt atcccggcat tgaacaaaaa acggggatcg tcccaaacgg
    24601 gtatgatgaa gaagactttg cagggattac agaatcaaag cccgatattt ttaccctgtc
    24661 atatataggg acattgtctg actcctatcc gctgaacgga ctgctggcgg cactttccga
    24721 actggaaaaa aaggaagcag cattcaggct cagatttgca ggttttgtct cagaaaatca
    24781 aagggtcctt gtttcgtccg cacttggcca tggcaaagtt gaattcattg attataccga
    24841 ccataggtca gctattggat atatggctgc ttcctcaatg ttaatcctga tcatacccaa
    24901 acattcctca tccagaagta tagttaccgg caagctcttt gagtacatgg cttcgctgaa
    24961 accgatactt tgtctggggc ctgccgatgg tgatgcggcc gaaattcttc tggagtcggg
    25021 gcataaatca gttttcaaat gggacgatca taccggaata agcgatttca tcggcagggt
    25081 aatggaacat cccggcgaac tggcgataaa aaaacccctc agttattcga gaaaagcact
    25141 aaccggcagg ctgtccgaaa tactctcatc actctgatca gacagaactg accgggggct
    25201 gtcatcattc cttcttcaac ttcataaata agtttcataa atttacacca ttacttgtat
    25261 tatgaaagca ggattcatac tgaatggtgt atttgtcaat gatcccaggg ttctaaatga
    25321 agcccgaatc ctggccggca acggatacca ggtctatgtg ctgaatcttc cgcctgacaa
    25381 gaatgttccg cgattcgttc agtttgagga caatatcttt ttggttcaca gacggatccc
    25441 gaaaaaggca aacaatgtct tattcgccct tgagaatacg tttgcactgt ttgattattc
    25501 ctggaatatt tcaataaaaa gattcataaa aaaatacggg atagagattc tgcatgcaca
    25561 cgatctgtac atggcaaggg ctgcaaaacg ggcatcggac cattataaga tccccctggt
    25621 cctcgacctc catgaaaatt accctgcagc aataaaagaa tatcattggg caaaccgctt
    25681 cccctccagg ttgatcgtaa ggccttccag gtgggaaaag aaggaaaaaa aatatctgtc
    25741 gtacgctgac aggataattg ttctcagcag ctcttacagg aatcacctcc tggccaagta
    25801 tcccggaacg gatccggtcc aggtaaaaat ctatcccaat gtccctgatg taaaaaggat
    25861 gtcatcatat gccatagaca gggacatttt acctccatat gacaggaaga tcattttcta
    25921 cttcggggtg atcagtcaga gaaggggtat tatgacaacc attgaggctg cagagatact
    25981 tctcagggac aatcagtcaa ttcatcttct tttgatcggc cccattgaca aggctgagaa
    26041 agaccgtttc atcaggttat tcgccggcag tgagattagc gggcatctca cttattatcc
    26101 ctggaaggat atcggtgaat ttccttcata tgttcacgca tcggcagtct gcatttctcc
    26161 catcattaaa aatccgcagc atgaatcagg ggtagcgaac aaggtgtttc aatatatgct
    26221 gttcgggaag cccatcctgg tttcagactg tgaacctcag gccgatattg tcagtaaaac
    26281 aggttgcggg ctggttttta aaagcggaga tcccgtcgac atggcagaaa aactttccga
    26341 gctcatcaat gataatgaaa aatgcattat catgggcgaa agagggaagc aagctgttat
    26401 gaaagaatac aacacggtaa aacagggtac ggaaataata aaagcataca gggaactgtc
    26461 tgcatctggg aaaaagtctg cggatacctg aaattcagct tcggatggaa catatgcatt
    26521 ttgtgatcac gcaatttaac ctgaggaaca agcctgtttc cggaagtgcc gcttatgatg
    26581 aatggctgag ctggaccagg agcaggatca gtattttccg gaactactgt ctcccatcgg
    26641 tgttgaatca gacatgccgc gatttcagct ggctgatatt ctttgattct tccacacctg
    26701 aagaattcgg ggagttcata ggttttcttt cttcgcatga cctaatctca gtctgctaca
    26761 gtgatggaat ggatgatttt tataccggtt atatcaggga ggtaagaaaa aaagccggac
    26821 caaccactaa atgggtaatt actaccaggg tcgacaatga tgatagtctt cacagggatg
    26881 caatactaac cattaaggaa aacttcattg caaagcatcg ctatcttatc tcccttgctt
    26941 ccggatatgt tctggatgtt aatgaaaaga aactttctca ttatttttat ccgatgtctc
    27001 catttctaag cctgattgaa gataccggaa aagaaattga ggggatttat tcaaggggtc
    27061 acacgaaatg gaataatctg cgcctgttta tattcaggga attgtggctt gaatacttta
    27121 atcccaaagc acgcgtttca agatttatac tgaagaaacc attatggatg caaactgtgc
    27181 atggggaaaa tgtcagcaat agcttttaca ggggcttccc ggttttgaga aaaagggatc
    27241 tcagcgattt ttcactggaa ctgatcacga acaggatgac tttcggaacg attacaagat
    27301 attgtcacta tgtaacctgg aagcgataca tgaaatgtct cgttatcaaa ttcctcataa
    27361 ataaatgaac aaggatgaag aatcgggtaa tggtcatacg aaaacttttt ccgaaaacct
    27421 ataattttct ggtttatctt attaacggat ccaggaagat cttcacacca tattccatat
    27481 acattaccga ttccctcggc ggcagtttca agacatatct ttcagatcct gtaaacataa
    27541 accgggaaac ggaactgaag cgcggccttg atgcagagtc ggaaagaaca ataaacaccg
    27601 tactcgaaag gataaagttt tatcccgacg aaaagcacag gaaaaaagta tcgaagagcc
    27661 agcctgtcgc aggcggtctg ttgcctgttg aaactgcagt gatgaaaaaa cagattgaga
    27721 attccctttc gggcgaccgg aaaaaataca aacggatatt ccgtcatctt gatgaatcgg
    27781 tattctattt ttatcatggt ttgcattttt taccggaaaa cgtccttaac tacctgaaaa
    27841 ataaggatct tatcgatata ggcgctcata gcggagactc agccatcgcc ttgcggaaat
    27901 tcggcttcag aaaaacctac tctgtcgaaa tgtcgctgaa atccattgat caatataaaa
    27961 agaatatggc tcaactgaag atcagtgaca acagttttga gctgatcaac gtctgcgttg
    28021 cagacgcgga taacaaggaa cctgcgaaat ttgccgatac cggatccacg gggctgtcac
    28081 ttctgaggaa caggggaaag tatgactgga tcaccgttga acagaaaacc cttgacacga
    28141 tagcttctga ataccgtatt tcgccgggat tgatcaaagc agatattgag ggcatgagct
    28201 tcgatttcgt aaagggcgct gtcgggacaa tgaaaaccca ccgtcccgta ctggctgttg
    28261 ctatatatca taatccccat gaactttttg aagtaaaacc cttcctggag aaagcacttg
    28321 ataactatac ttttttaatc aggaagcttt cggctgggat agaaaacaat ttatgccatt
    28381 cagatatatt tttaataggt tttcccgacg aagccgcagt gtaaacaatg tacacaaatc
    28441 accctcatga ccactcatta acgattcagt aaatccatct gatgcaaaaa cttataaaaa
    28501 catctttcag ggacagtttt atttacggac tgggaaatat atccgtgaag gtaataggtt
    28561 ttttgctgat acctctttat accgatccaa ggtatttttc agttgatgag ttcgggatta
    28621 ttgccctgct cgatatctcg ggaatcgtac ttatatcgct gatggcctcg ggtctcccgc
    28681 aaagccttat gagatggtac tgggatgatg agttcaggga taatcagaaa ggtatatttt
    28741 tcatgtcgct cagcaaccag gttattgtaa gcatctcctt ctgcattatt cttataccgt
    28801 tagcaggcag attttcgaca ctgatatttg gaagcgaagg gtggagcaat gcactgaaga
    28861 tcctgatatt gtccaccgcg ctgcaggcaa taaacaacat tatcaattcc ctgatgagga
    28921 ttcagtcaaa ggctttgctg ttttccgttg ccaacttcat caagcttgca gttgtgctca
    28981 tccttacagt ctatctgatt gttttccggc aggccggggt aaaaggtatc tatatcgccc
    29041 aggttgcagg aaacctgctt tttataatcc tgctctcggt ttatgtggca aggaattgca
    29101 ggatattttt taacctgcag gtattcaaat cgatgagtcg ttatggattt ccactgctta
    29161 tttcaaactt tgccgccgca tcacttgctg tcatcgacag atattcactt aattcccttg
    29221 cactgttgaa atatgtggcc atttatacca tggcatacaa aatctcatcg gttttgaaac
    29281 tggtaattgt cgattcgatc aaaatggcaa tcacaccaat ggtaatgcaa aaaatgaatt
    29341 ctcccgataa taaaagattc tattcaaaga cattgttgta ttcatccttc gtattaatgc
    29401 tgggtatcat tacattatca ctattttcat atgaaataat aaagattatt tcaagatcgg
    29461 cggagttctg gattgctttt attgccgtcc cggttctttc tctgtccgtt ttttttgcaa
    29521 atatgaggga aacaagcagt tatggcctga ttatcaacaa aaaaacaaat attgtcggga
    29581 tttctgtcat tgcttcaagt gttctgaaca tcattctcaa tattatcctt ataccggtat
    29641 ggaacattac aggagcggca attgctacaa tcaccactca gttcttttac tggtttctaa
    29701 actattacta ttcacagaag gaatatcaca ttccgtatga actgaaaaaa atcgcaatca
    29761 tgttcatatg cggcggcatc ctgtcctttg ccggtcttat gctgaatgaa ttgcacatcg
    29821 ttccacgcct cctcataaaa actgcctgtg taatcagttt ccccttcatc ctttacctgt
    29881 tcaatttcta tgaacccgtg gaattacagg caatcagggg attcatttca aaatggtcga
    29941 ggctcaggaa cttaagggac aacctcaggt cattgaaaaa tattcagtag aggatttggt
    30001 tgatttaaat ccatttagct gttttcgttt gaggtaattc aatacttttg cattgaattg
    30061 aaaaaacgga tctcaaaatc gtttcccatg aaaataaacc aggatctccg atcggcatta
    30121 cctcatttgc tggcagttat tgtatttatt atcatttctt tcatttattt ctaccccgtt
    30181 ctggaaggca aggtcctgaa agcaaatgat tccacagttt catcaataaa ttccaaggag
    30241 atacgtgatt tcagggaaaa atatggcagg gaaccccttt ggaccaattc gattttcagc
    30301 ggaatgcctg cttacctaat atccacaagg tacccgggca atctcatgaa gcatgtcgac
    30361 accgccctca ggatatttca gacacctgta tccgttttga tcctggcaat ggcaggtttt
    30421 tatcttctgc taataatttt tggggccgat cctcttattt cggcggtggg tgcatttgca
    30481 tatggtttct cctcatttct cttccagatc cttgcagcag ggcataacac gcaggcgata
    30541 gccctggcat atatggctcc gatgatagga tcggtttatt atgcatataa acggaatgcc
    30601 ctggcgggag cagttctgac agcagttttc ctgtcgctcg agatcctggc aaatcatcca
    30661 cagataacct actattccat gatatgcctg atagtcttta taattactga actcattttt
    30721 tcaatcaggc aaaaaacaat cgccagattt cttaaaacct ccgccctgct tttaacgccg
    30781 ctaattattg caatcggcat aaatttcgga aatctttaca ccgtatatga atatggaaag
    30841 ttctcaagca ggggaaaatc ggatctgtcc gtgagcgaca aaaatgtctc gaaaggtctg
    30901 aacagggatt atataaccta ctggagctat ggtattgatg agacatttaa ccttcttgtt
    30961 ccgaacttta aaggcggatc aagcaagccc tttgacagga attccgagac ggtaaaagcg
    31021 ctcaggcaaa acaatgcttc atctgcagcc ggacagttgg tcaaatactg gggaacccag
    31081 ccgggaacgg acggaccaca ttatatgggg gcaattgtga tattcctgtt catatttggc
    31141 ctggtgatcg taaaaggacc tgagaaatgg tggcttctcg ctgcctcggt tctttccgta
    31201 atgctcgcct ggggtaagaa ttttatggta ttcactgatc ttttcatcga ttttttcccc
    31261 ggctacaata aattcagggc agtaaccatg atattggtca tcgcccagtt ctgcataccg
    31321 ctgctcggaa tccttgccct gaaaaagatt ttttccgtga atctgtcaaa aaaagagatc
    31381 ttcagaggat tgaaaattgc cgccggaata acgggagggc ttttgattat gttcctgttg
    31441 ttacccggaa ttgccggatc atttctgaat ccctacgaat ccgactatcc cgactggctg
    31501 agatcagctc ttgttgcaga tcgcaagagt cttctgagag ccgacagcct gagatcgctg
    31561 atcctcatca tcctgtcagc cggcacgata actgcatttc ttcagaataa actccgcagg
    31621 gagtattcaa ttataattat aggcctgtta atccttgccg atctctggag tgccggaaaa
    31681 cgttatctcg attcggacaa atttgagagg ccctcggtca ttcagaaatc attcactcct
    31741 tcagttgccg atgaaatgat cctgcaggat acttcatacc acagggtgct aaacctttcc
    31801 gtttccacct tcaacgataa tacacccacc tcctattttc acaaatcaat aggaggatat
    31861 cacggagcta aaatgaaaag ataccaggag ctcatcgaca cttcaataac ggttaacatt
    31921 gaaagtttca ttgaaaaagc aaggaatgca aatacaatcg ctgacctgtt gccggtattt
    31981 gataacacca gtgcaatcaa tatgctaaat gccaaatatg tgatttacaa tccggatgct
    32041 ccgcccctgg taaacaggag ggcgcttgga aatgcctggt ttgccggaaa ggtgaaattt
    32101 gcagccaatg ccaacgagga actgtcgtca attaattctc tcgatccttc ctcggaagcc
    32161 gtggttgata ccgtgttcag ggattatgtg aagtctctct cctttcctta taatgaaggt
    32221 gacactattt accttgtatc atataaacca aatgaactga tatataaatg ctcctgttcg
    32281 ggcgaacggt ttgccgtgtt ttccgagata tactatcctg caggatggaa atcgtttatt
    32341 gacgggaagg aatcacccca tttcagggtc aattatgtac tgagggcgat gattgtgcct
    32401 gaaggcagcc acgaaataag gttcagtttt gagcccgatt catattttgc aggtaacagg
    32461 gtttcacttg caagttcatt gctgctgata ctggctattg cagtatacgc tggtgcggaa
    32521 cttaagaaaa agctaaaatc tccctgaaat gaacagacca ggtgaatgtc cgctatgtgg
    32581 tgcagaaaag gtcagttatt atctcacctg taccgatcat ctggtcagtg gggaaaccta
    32641 cgacttgttt atctgtaatg cctgcggttt tgtcctcacc catgatccgc ctgatgaaga
    32701 agcaatggga agatattatg catctgaaga ttatgtttca cacagcgaca caaaagccgg
    32761 agtgataaac cggctttatt cactggctcg aaatataatg ctttccagga agctgagaat
    32821 catttcagca gcttccggat taaggaaagg cagtatcctg gatattggct gcggcacagg
    32881 ttatttcgct gcaaaaatga aagcaaacgg atggaattca acaggtattg aacctgactg
    32941 caaagcgagg gaatttggca ccaggcaatt cgggctgagt atactggaac ctgaaaagat
    33001 ctcatcactt ccgggtgaaa gctgtgatgt gattaccatg tggcattccc ttgaacattc
    33061 tcacgatccc ttcaactata tgcaggaaat cctgcggctt cttaaaccgg aaggtttatt
    33121 catatcagca ttacccaatt gcggatcgta tgatgcaatg cattttggtg attcgtgggc
    33181 tgcttatgat gtcccccggc atctgtggca tttcactccc gaaacctttg aattattcgc
    33241 tgcaaagaca ggttttaaag tgacgcatgt aagcaccctt ccctttgatg ttttttatat
    33301 ttcattgtta agtgagaaac acaggaatag aaaattgtat cttctctccg gcctggcaaa
    33361 aggatccttt ttttcagtca gggctgtttt taattacagg aaaagcagtt cccttgttta
    33421 tcatctccgg aggagatgac gataatgcat tactttaact gtagcctgca gggttatcga
    33481 agcataatta aatatccctg attatgggac acgttttttt cagaaattgc tcaatagcgg
    33541 tcgagccagc ggtattttct ggcactgtca ccgaatttga cagccaatga cagttcatgg
    33601 gtcccgaatg tcaatctttg aatttcagaa agggtaaaat cataggaata gccaatgaac
    33661 agattcgtat atctgacccc gaggtttgca accaatgctt tgcttgtcct gtaggaaaca
    33721 cctgcccata ttttctgatc atagttgcag gttactccaa tatcagccat tggtttggtc
    33781 tgttctgaca tcatgataag caccgaaggc tggatctcgg ttttaacatc agcattgaaa
    33841 ttgtaagatc caaagaggta atagtgtctg tccattgaat agttacgata accttcccct
    33901 cctattctgg cgacggcccc gaacaactgg gaacttgtaa aaccgaaact gaagtccgga
    33961 ttaagcacat aaatgccaaa atctgcatca gggacaaaaa tacctcttct caggttattg
    34021 ttaagccatg gttcatttgg atcttcgaaa ttgatttctt tttcattgat cttgaaatga
    34081 tatcccgtga atgccagccc caaagatacc tgtgtagttc ttcctgtcca catatgatag
    34141 gagtatgcag cctggaatcc ggttctctgg accaatccgt gcctgtcact gaatacatag
    34201 cctcccagcc cgactttacc gtcccctttg ggcctgaacc tgttatttcc tgaacggtca
    34261 gcctttatgg aatattttgt tttcagaata cgtccatgtc cgctgaaaga ataggttcta
    34321 ggggcgccgg aataacctat ccactgctcc cttgccgtaa gattaatgtt tgtatagcct
    34381 tcgctgccgg caaccgccgg atttataagg aatttattct gcagatactg actgtataat
    34441 ggcagctgct gccccgaaca taagggccag aagaaaaagg cagccactat tgaagtcagt
    34501 tttttcatgc ggtaaattat ttgacaattg ttacatggcc tattacagga cgtgttccgt
    34561 tgttaagatc aattatgtaa tgatatgaat cgaccggaag cattcttccg ttgcttcttc
    34621 cgtcccatgg cctcggatat cctcgttccg acttccagac catttctccc cagcggttaa
    34681 agatcattac ttccatcgcg ggatacagct ctttattatg aatgttccac acatcattta
    34741 caaggtcgcc gttcggagat attgcatttg gtatctccag acacaatgga ttttttgcct
    34801 caaggatcaa cgatttttcg acggaacaat tgttcatgtc agtaaccctt atgctgtaaa
    34861 ggccgcccgt tgcattggaa agatcaggat ccgttgaatt gtcggaccac aggtaggaat
    34921 atccgccatc tgttcctccg gtcacccgtg ttttgatctc tccgtcgggc atgtcaggac
    34981 aggttggaag agttacatcc aattcaacca ttaatggctc gggctcggta aagatgataa
    35041 ctgaatcagt cctacagttg tttgaatccg ttattatcac cctgtatctt cctgctgcca
    35101 gtccttgcct gctattaccg agtacgccgt cggaccagag ataggtaacc gctcccgcat
    35161 tattgaccgc ctcgatactt atttctcccg atttgtcgcc attgcatctt atatcttcaa
    35221 atgcagtaat atcgattgtg agttcaccag gcggttcaat gagagtcact gtttcgctgc
    35281 ctgtacagta gttgctatcg gtaataagta aaacatactc acccgccttc agtcccgaga
    35341 tttcattccc ttcccgtgaa aaaccatccg gaccctgcca gacgtatgaa tacggaggag
    35401 tgccgctgag gggattgaca tgaattgatc cgtcggacct gccgaaacag cttatattga
    35461 aatttccgta agctgaaagt attttctcaa aggtaagggg tggaggcagg tttatgcgaa
    35521 ccgatccttc ctttgtacag ccattggcat cagtaacggt tacggaataa actccctcag
    35581 tcaggtttgc tatatcctgg gtatcctgac ctcctgacca gatgtaatta tacggagcaa
    35641 ctcccccgga aaccgtgaga tcaactgagc cattatcata tgtgtccgac tggcatgtta
    35701 tatgtgtcgg aacaagcttc agctgaatct ccggaggttc cgtaaggtta atatcttttt
    35761 ctgcattgca gccattaaga tcggataccc gcaaatgata ggaaccggca gaaagggata
    35821 gctgatcttt ctcaccatcg attattcctg atccgttggt ggtggtccat gaataagaat
    35881 aattcccgat acttccgccg ctaaccgttg tttcgaccgt tccggttcct ccatgacagt
    35941 cgatattaaa tcctccgtgg gatgacaccg aactttgcag cgatacctca agtttatcag
    36001 gctgggtaag cgtgaaaatc ggctcaggca taaggataca accattaata tctgtcactg
    36061 tacatgtata aacgcctgac tttaattgcg atatatcttt tgaccgggat tggaaaccgt
    36121 ccggacccac ccacaggaag tcgtaattac ccgaacctcc tgcaatatca attttgatga
    36181 aaccatcgtc ggctccgtaa catgagatcc cgtaattacc atcagggctc ctggacacct
    36241 ggctgccagc aagttccatg cccggggggt cggtaagaac gatactctcg atttgttcac
    36301 aaccagataa atcagtaact cttacataat atttcccggc aggaatgcca tcaagcctgc
    36361 ttccggtgtt attgcccggc aatgtacccg attcgggata ccagtaatat gaatatcctc
    36421 cgttaccacc gctaaccgag gctacggcat aaccagtact ataacccttg cagtcgatat
    36481 tcgaaccgct gtaatcactg ggggtaatgt taataatcaa tggaggaggc tctgtcagta
    36541 tggcgtccat agttaattcg catccattga aatcccttat tatcaattta tagcttcctg
    36601 ctttcagatc tgtacaaagc agataggttg aaggatcatc agaagcataa atattctgga
    36661 attccccgct gtacagggtc tcggtttcat tgtaaaccag ccagtatctg aacggaggag
    36721 tgcctccttc cctgacatat atcctggcga ctccgtcatt gcccccgggg catgtcacat
    36781 gatgaccggt cgatttcgga agggggataa ttcttggttt ggcactcaag tttgctatgt
    36841 tgaatgatgt ttcattcatg cagcccaggt tgtc
//
LOCUS       DAOOYG010000015        33918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1082_length_33918_cov_6.599150, whole genome shotgun sequence.
ACCESSION   DAOOYG010000015 DAOOYG010000000
VERSION     DAOOYG010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1082_length_33918_cov_6.599150"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..453
                     /locus_tag="PK288_02710"
     CDS             178..453
                     /locus_tag="PK288_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17859.1"
                     /translation="MLSRMSDQMREENLARFEANGEVYDRVSRQFSDNILGIDRYYDP
                     HEGREVELPSGYNYAWSNNNGEYIVTDSPDLNPNVGSNLHWELMPRK"
     gene            complement(472..1044)
                     /locus_tag="PK288_02715"
     CDS             complement(472..1044)
                     /locus_tag="PK288_02715"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HPM17860.1"
                     /translation="MKTINKNTGNLFLLTALLLISVTGYSQDFGNEKLSRKEKKQLRE
                     AELNANYNLLNDLVTSRSFVVEADFLENKYGEKVPVSSSINFIMVNADEGVLQTGSNF
                     RIGYNGVGGVTAEGTIGDYQVVKNPKNLSYNVKFHIVTNLGSYDIFLTVFSDFRARAT
                     ISGVTSAKLTYTGRFEDLSRSKVYKGMRTI"
     gene            complement(1108..1965)
                     /locus_tag="PK288_02720"
     CDS             complement(1108..1965)
                     /locus_tag="PK288_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPM17861.1"
                     /translation="MKQLSFFLIVMITGGLLSAQESRVYDQLTMQSKILKMERKFAIY
                     LPAGYETSQRDYPVLYLLHGAGDDQTGWVQFGEVRHIADKAIKDGVSTPVIIVMPDAN
                     TGRRGYSNDVRNEWRYEDFFFEEFMPFIESTYRIRKDKEFRAVAGLSMGGNGTLIYAL
                     HHPELFGSACPLSAGAGPVNLANVKRSMGADSSKIKKEQYDAYFQKYDIPTLINRIPD
                     DRKKDVRWYIDCGDDDFLYEGNAMLHIYMRKKEIPHEFRVRDGAHNWTYWRESLPEVL
                     HFISAGFHR"
     gene            complement(2122..4023)
                     /locus_tag="PK288_02725"
     CDS             complement(2122..4023)
                     /locus_tag="PK288_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPM17862.1"
                     /translation="MKRILLLFVSLFWAFVSHSREYHVSVSGNDSYDGSPAKPFRTIN
                     AAVQAAYPGDTVTVHAGTYREWINPVRGGESDSRRILYRAAPGERVEIKGSEEVRGWT
                     KVNRNGVWKVVVPNSVFGSYNPYQDSVFGDWFNDRGRIHHTGELFLNGKSFYEKETLD
                     KILDPVPDTMIMDREGSVYTWYCESNRDYTTIWANFHKFNPNRELTEISVRPTCIYPG
                     KPGINYITIRGFIISQAATQWGAPTAGQVGMIATHWNKGWIIENNVIRDSKCSGITLG
                     KEHGTGHNVWSADKGNIYNDGNIHYIEVTFRVLRNGWNKETTGSHIVRNNVIFNCEQT
                     GICGSMGAAFSIIENNHIYNIWTKRQFAGAEIGGIKFHAAIDAIIRNNRIHSCGRGLW
                     LDWMTQGTRVSANLFYDNDLEDIFLEVNHGPFIVDNNVFLSRAAIKTQSEGGAFVNNL
                     IAGTVHNWPEPNRFTPYFLPHSTDMAGLTTIYGGDDRYYNNIFIGPGTEKTGENENYG
                     LKGYGNARLPVRIDGNIYCNGATPSQKDENFLFFPSHGQGVNLFEEDGHGYVILSYDS
                     SFTSHRVKTVTTEMLGKAKIPKADFDNPDGTVLVIDRDFFGDKRPPDGNLSGPFINVL
                     PGENKFRLW"
     gene            4194..5627
                     /locus_tag="PK288_02730"
     CDS             4194..5627
                     /locus_tag="PK288_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM17863.1"
                     /translation="MRPTRRNFLKLSGLAGISLAASNALKAIVPQTEDRKSGKNSIPG
                     SSSEISAQTFNMCGYAAPRLDKVRIGFIGLGGRGRLHESIMSIIEGVEIKALSDILPD
                     RAAKAREIAVKAGFNPAMYTDSPDAWKKLCERNDIDLVYICTPWNLHTPMAVYAMEHG
                     KHAAIEVPAADTLEECWQLVETSEKTRKHCMMMENCCYDFFELLTLNMARQGFFGELV
                     HCEGAYIHNLLKSNFNKDGYWNMWRLRENASRRGNLYPTHGLGPVCQILDINRGDKMD
                     LLVAMMSRDFAMNETARKFAATDSFFRPFVDNPYRGNMTTTTILTNKGRTIMIQHDVT
                     SPNVYSRIHKVSGTRAAALKYPLPPKISVEDEWVTDEEFRNIEEKYTPPIIRKVGELA
                     KKVGGHGGMDFLEDWRLIDCLRNGLPLDQDVYDAALWSAVAPLSEWSVANKCNSVEVP
                     DFTRGAWKTNAPVDISLSQGGNTGVRG"
     gene            complement(5645..6553)
                     /locus_tag="PK288_02735"
     CDS             complement(5645..6553)
                     /locus_tag="PK288_02735"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPM17864.1"
                     /translation="MKTSRRTFIRAGAMAVVAASVRPSSLFASSKAKGIIGLQLYSIR
                     EDMARDPLGSLTQVAKMGYEYVEHANYVNGKFYGYSPGEFRKVLDGLGLKMISGHTVM
                     GKQHWDEARKDFSDSWKQTVNDAAVLGQEYVVSPSMDMTMRNNYDDFRKYMDVFNKCG
                     DLCKRHGMKFGYHNHDFEFSEKLRGEKLFDIMMKSIDPDKVVIQLDIGNMYNGGAVAL
                     DVVKQYPGRFENLHVKDEILSSGGPEKYESTIIGKGIVNAREVVDLATRIGGTRVYII
                     EQESYQGMSPMDCVKEDLRIMKEWGY"
     gene            complement(6845..7339)
                     /locus_tag="PK288_02740"
     CDS             complement(6845..7339)
                     /locus_tag="PK288_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17865.1"
                     /translation="MVREAPEKKPVAVVRPGIISAIVLKSLFVLVLLFSTAGESVAPS
                     RNYLVIHKPGGINPFEPLMHAIGMVETMGNTMAFNEYEGAAGIFQIRQVRIDEYNRQT
                     NSCFRLSDMFDYENSRKVFLHFASKIGPYNFEKIAKSWNGSGPMTEFYWRRIRSQLQK
                     IQPA"
     gene            7921..11124
                     /gene="carB"
                     /locus_tag="PK288_02745"
     CDS             7921..11124
                     /gene="carB"
                     /locus_tag="PK288_02745"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPM17866.1"
                     /translation="MSKHQDINKVLIIGSGPIVIGQACEFDYSGTQACKALRSLGYKI
                     VLVNSNPATIMTDPEIADITYIEPLNEYALTEIIKKERPDALLPNLGGQTGLNLSSLL
                     AKKGILKKYNVRVIGVNIDAIERGEDRIAFKETMNRLGIGTPRSKAVNSVEAAENAAA
                     ELGYPVVVRPAYTMGGTGGGLVFNVEELRTIATRGLSASLVNQVLIEESVLGWEELEL
                     EVVRDSKNQMITVCFIENVDAMGVHTGDSICTAPMLTISEELQKRLQKYAYDIVEAIE
                     VIGGTNVQFAHDPVTDRVVVIEINPRTSRSSALASKATGFPIAFVSALLAGGLTLDEI
                     PYWKEGSLDKYTPSGDYVVVKFPRWTFEKFEGLEDRLGTQMKAVGEVMSIGKTYKEAF
                     QKAIRSLETGRYGLGFAKDLNRKSLEELMNMLNHPSSERQFIMYEALRKGAEIDELYK
                     RTFIKRWFIQQMKELVEEEEEILSWKGQMLPDDMLVQAKKNGFSDRYLSALLGVGEKE
                     IRTRRISLGLTGAWEAVPVSGVKNASYYFSTYNASGKIPVSSKKKIMVLGGGPNRIGQ
                     GIEFDYCCVHAAFAIRDAGLESIMVNCNPETVSTDYDTSDKLYFEPLTVEDVLSIYNN
                     EKPEGVIVQFGGQTPLNLANELAEAGVRILGTTPETIDLAEDRDRFRQVINRLGIPQP
                     ESGMASNLQEALRIAERIGYPLMIRPSYVLGGRAMKIILDEQMLEEYVAKAAGVSPDR
                     PLLLDKFLEDAVEAEADAISDGVDAFVPAIMQHIEYAGIHSGDSACVIPPVIISRKHK
                     KTIYDYTKKIATELKVVGLMNIQYAIYDDTVYILEANPRASRTVPLVSKVCNISMARI
                     ATQILLGQKLREFNLKNKTFRHYGVKEAVFPFNMFPSTDPLLGPEMRSTGEVLGMADS
                     FGMAFFKSQEAAQSSLPVRGTVLITIADRDKQRIIEAARNFRDMGFRILSTTGTKAFL
                     EQNGIPAELALKVHQGRPNIVDAIKNGEIDLLINTPAGRLSEYDDSYIRKNAIKYKLP
                     YITTTSAALAATKGIRERQNEAYFVRSLQDYHSSIGE"
     gene            complement(11235..11573)
                     /locus_tag="PK288_02750"
     CDS             complement(11235..11573)
                     /locus_tag="PK288_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17867.1"
                     /translation="MGSLIIFQIMSVTAAVILIIALLVVAGLIGYLTAWFYAKSVYTP
                     VIRKLEDEKVQLGREISELKEDIGKLEKKISGLNEKISGLEKEVADKEKEISDKVNEI
                     QELKKKQVKE"
     gene            complement(11578..12087)
                     /locus_tag="PK288_02755"
     CDS             complement(11578..12087)
                     /locus_tag="PK288_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM17868.1"
                     /translation="MRILISGLIAFVIWSFFSTWLYTDVLRHAARKPEPVQAVPVPKI
                     SVADSLLEINAMMPEDLVICFEFDKFKLKPTPQFESGLTEFKDWLDKYPESVLLVTGH
                     TDLIGTREYNQKLGMERARATQKYLETKGFPSSRMIVESRGEDEPVAGYIKAEDRAKN
                     RRTVISIKK"
     gene            12371..13039
                     /locus_tag="PK288_02760"
     CDS             12371..13039
                     /locus_tag="PK288_02760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM17869.1"
                     /translation="MKKIPKSILFCFFICLINIPAQTQSGKADPSLDEKVKKFLNEHA
                     RHWYDMNVPWVDGQLLHDLVLKGNYKSALEIGTSTGHSAIWIAWALSKTGGRLITIDI
                     DERRHRTALENFRQAGLSDYIDARLANAHDLVKELKGPFDFIFSDADKDWYKNYFVIL
                     DPKLKVGGCFTAHNISERVRGSYGSYSGTAVFLDYVKSLPNYRTTVNSAGGGVSISYK
                     TAEK"
     gene            13048..14403
                     /locus_tag="PK288_02765"
     CDS             13048..14403
                     /locus_tag="PK288_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPM17870.1"
                     /translation="MMTGNALERRLGLFPATNIVIANMIGAGIFTTSGLLMSGLHDPV
                     IMVLLWAAGGFIALCGAFSYGELGAAMPGAGGEYLFLSRLYHPLFGFLSGWVSFVVGF
                     SAPIAASALGFSEYFSRALPGFTLWMQDSAMIQPEMTRKILSVSVILVFTLIHYTGIR
                     YGARIQNVLTILKILLIVILLTAGFASGQGEFFNFSSGGTIPQGLAGWKTMGLSLMWI
                     MFAYSGWNASTYLGAEIRNPETTLPRSLIFGTGIVTLLYIGINILYVYAISPAEMKGV
                     ISVGGLAMGNLFGKPAEVLFSVLIAFALFSSLSAFIIIGPRVYYSMAKDGLFFPFAAR
                     IHKKFHVPSNSILLQGLIAVILAFSGTFEQVLTYMGFALGIFPLMAVAGVWKLRRNNS
                     GAIRLKGFPFTQVVYLTAGSLILMLSFMERPVESSVAILTVLAGIPAYYLFRKNSGAA
                     G"
     gene            complement(14367..15149)
                     /locus_tag="PK288_02770"
     CDS             complement(14367..15149)
                     /locus_tag="PK288_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPM17871.1"
                     /translation="MKPYKENWQSATELLIGREKLDRLKASSVLIVGLGGVGAYAAEM
                     ICRAGTGSITIVDGDTIHPRNRNRQLIALTSTEGSPKAVLMGNRLREINPELDLTVIQ
                     EYIRDERMCEILDRGFDYVVDAIDTLSPKVYLIYNSLKRGLPVVSSMGAGGRSDPLRI
                     MINDISETKGCNLARMLRKRLHRLGINSGFTAVYSPEPVDKSRVMPVSGERNKASVVG
                     TISYMPPAFGICCASVVIRDLAGIPRQYSGTQPAAPEFLRKR"
     gene            complement(15228..15911)
                     /locus_tag="PK288_02775"
     CDS             complement(15228..15911)
                     /locus_tag="PK288_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPM17872.1"
                     /translation="MNSPRPGDFIDIHTHGASSSPGIFRVEVLMAHEAVRPVEQRGIA
                     YAAGIHPWHLNEADRSRLTEFMRSVAEHPLVIAIGEAGFDRLRGPSMELQREVFDEQA
                     EMAEKLGKPMVIHCVKAWDEFLSAHKRLKPTANWLIHGFRGKKELASQLLSRGFYLSI
                     WFEFALRPESAGLIRFIPADRLFLETDGADTDIRDIYRKAAADLGVSTDELKITMYNN
                     YKKFLGVRS"
     gene            complement(15908..17431)
                     /gene="gltX"
                     /locus_tag="PK288_02780"
     CDS             complement(15908..17431)
                     /gene="gltX"
                     /locus_tag="PK288_02780"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPM17873.1"
                     /translation="MTMNGIRVRFAPSPTGPLHIGGVRTALYNYLFARKHGGTFILRI
                     EDTDQSRYVDGAEDYIIRALEWCRIDVDEGINEGGSFGPYRQSERKHLYRQYADMLVD
                     RGHAYYAFDTPGELERMRIESEKKGETFIYNSAVRDKLVNSISLDPVDWNARLDRGDP
                     FVIRFKMPRDEEIHFDDLIRGHIVVNSDTLDDKVLFKSDGMPTYHLANVVDDYLMKIS
                     HVIRGEEWLPSLPLHVMLYRAFGWEAPLFAHLPLLLKPDGRGKLSKRDGDKLGFPVFP
                     LYWPYGETARGYREDGYYPEAFVNMLALLGWNPGTDQEIFSMSELINSFTLERVGKSG
                     MRFDPEKARWFNHVYLQKRSNNQLALEFREFLRARNYHTDIVRLEVLIGLVKERVSFV
                     KDLWDQTDFFFRAPENYDQEAVSKRWTESTPAMLRELIAVLLSTADFSPSGTEASVSS
                     WIKSSGYNTGTVMNAFRLVIVGALRGPRVYDIISWIGREETIKRIEKGISIIGSKKK"
     gene            17651..18676
                     /gene="tdh"
                     /locus_tag="PK288_02785"
     CDS             17651..18676
                     /gene="tdh"
                     /locus_tag="PK288_02785"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPM17874.1"
                     /translation="MKALVKALPEKGLWMQEVPIPEPGVNDVIIKVRKSAICGTDLHI
                     YNWDQWAARTIKTPMIIGHEYMGYIESTGAGVRIFKPGDRVTGEGHLACGHCRNCRRG
                     KEHVCENTLGIGVNIDGSFAEYVRIPSSNIVPLDPRIPDDWAAIMDPFGNATHTALSF
                     PLLGEDVLITGAGLIGSMAVAIAKFSGARFIVASDLSDYRLEIAKKMGATLTVNPSKG
                     EKISDAVKKLGMRGFDVGLEMSGSPKAFIEMIGNMYNGSSISLLGILPSNFEVPWSDI
                     IFKAITLKGIYGREMWETWYKMEQMIIGGIDLNPVITHHFSIDDFQKGFDVMEEGNCG
                     KVILDWD"
     gene            18854..20044
                     /gene="kbl"
                     /locus_tag="PK288_02790"
     CDS             18854..20044
                     /gene="kbl"
                     /locus_tag="PK288_02790"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPM17875.1"
                     /translation="MYENFQEHLKATLDDIRESGLYKTERIIVSPQGAEIVLDTGQKV
                     LNFCANNYLGLSNNRLLVEAARSALESHGYGMSSVRFICGTTDLHKRLENMIAGFFGT
                     EDTILYAACFDANGGVFEPLLTDRDAIISDALNHASIIDGVRLCKAQRFRYENADMDD
                     LEKQLKLARDLRFRLIVTDGVFSMDGNVAPLDRIFELAERYDAMIMVDESHSAGVVGN
                     TGRGVTELFNLRGKIEIITGTLGKAFGGAIGGFTTGRKEIIELLRQRSRPYLFSNSIP
                     PLVAAAGIKMIEMMTGTNELQDRLHNNSRHFISRITGLGFDIKPTMSAICAVMLYDAK
                     LSQDFAALLLEEGIYVTGFSYPVVPKGQARIRVQLSAAHETEHIEKAIAAFEKTGRKL
                     GVIK"
     gene            20133..21020
                     /locus_tag="PK288_02795"
     CDS             20133..21020
                     /locus_tag="PK288_02795"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPM17876.1"
                     /translation="MNRILFTIYRIAVPKPLRTILLKRKIRRKILDQFEALPEISLND
                     DQREVVNYLRENQVSIFPYPFQKKYRPEEIRVFSDPDLGMKYVIHEGKQLYFRRRWNE
                     KRIRISYSELLKEQDPESPHRYITSDFSPGPGDVVADIGAAEGNFSLSVIDKVKRICI
                     FEYDRGWVEALNATFAGWKGKVEIIPKYVAGYDDERHIRLDTFFMTHNDITFLKIDVD
                     GYEKRVLEGCRSLLESKIPLRIAICTYHKNDDEAEFTRLLQDHGFSVSASRGYMINYY
                     DKKLKAPWLRRGLIRAVRN"
     gene            complement(21023..23527)
                     /locus_tag="PK288_02800"
     CDS             complement(21023..23527)
                     /locus_tag="PK288_02800"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPM17877.1"
                     /translation="MVKYTHDEAVEASIRYFNGDELAARVWANKYALKDSFGNLYEKT
                     PDDMHRRLAREIHRIEKKYKNPLPEELIYNVLKDFRYIVPQGGPMTGIGNNFQIASLS
                     NCFVIGNEGESDSYGGIMKIDQEQVQLMKRRGGVGHDLSHIRPKGTPVLNSALTSTGV
                     VPFMERYSNSTREVAQDGRRGALMLSISIKHPDSGKFIDAKLESGKVTGANVSVKLTD
                     DFMRAVENKTPFRQQYPINSDNPKFIKEVDAVELWNKIIHNAWKSAEPGVLFWDTIIR
                     ESVPDCYADLGYATASTNPCGEIPLCPYDSCRLLAINLYSYVEKPFTKEASFNFDLYR
                     EHVGLAQRMMDDIIDLELEKIDSILEKIESDPESDELKLVEKNLWKNIRNKSEEGRRT
                     GIGITAEGDMLAALGLKYGSDEATDFSVEVHKTLALEAYKSSTYLAKERGPFLIYDAE
                     REKNNPFILRMKEADPVMYSNMVKYGRRNIALLTIAPTGTTSLMTQTTSGIEPVFATC
                     YKRRRKVNPNDKGVKVTFRDEVGDSWEEFNVFHHKFVDWLRLNGYDPDVVTGMTDEEI
                     SAIIAKSPYHKATANDVDWIAKVRMQGAIQKWVDHSISVTINLPTDVKEELVSELYLT
                     AWKSGCKGATVYRDGSRSGVLIAGRNEIRTERPKRPKTLDCDVIRFNFNEEKWVAFVG
                     LREGRPYEIFTGLADEEIFPIPKTIIKGKIIKVKLDDGKTRYDFQYTDKYGYRKTIEG
                     LSHMFKPEFWNYAKLISGVLRHEMPIQDVVNLVQSLKLDSESINNWKNGVERALKKYI
                     PNGTKAKGKCAECGSDNLVYEEGCLICKDCGSSKCG"
     gene            complement(24147..27407)
                     /locus_tag="PK288_02805"
     CDS             complement(24147..27407)
                     /locus_tag="PK288_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPM17878.1"
                     /translation="MKRIIKTVFQVVLFLMIPAFLYGQGATTSGLNGRVTDGSGQPLP
                     GAAIVAVHVPSGTQYATVADNAGNYRIQNMRVGGPYSIRVTFIGFSTSNYTDIYLRLG
                     ESYVQNAELSEATTTLQEVVVTSGLRNSILSSERTGTQTNVSSRDLANLPSINRSITD
                     FSKYTPQSQGNSIAGRDGRFNTITVDGSAFNNNFGLSSNPLPGGSAQPISLDALEEIS
                     VNVAPYDVRMSQFTGASINAVTRSGDNTFKASVYTYQRPKSFTGNTVDGNEVANANTR
                     QLQNYGFRVGGPVIKNKLFFFVSGEYEKEIIPGVSWNPSQDGVANSNLKISRTRESDM
                     AMVRNYLMSNYNYDPGKYKNFDPFMNKNTKILVRLDWNINKNHKFTLRYNDVIGTSDS
                     ETNATSGPPNNSRGSGRISNQSLAFSNSFYGFRNTVRSVTGELNSTLGPKVSNKFLAS
                     YTYIEDKRTSPSKFFPFVDIWQDGDQYMSFGYELFTYNNRVANKTLSLTDNVTINLNK
                     HTVTSGLAYDNLYFYNSYIREGTSYYRYPSVDAFINGEDPTGFGVTYGYKGNDAPGAE
                     LTFGMAAVYLQDEWQVTPKLKTTLGFRVELPVYLNKLDNNPAISALTFIDGRRMDVGA
                     WPEVKPLLSPRFGFNYDVMGDRSLQIRGGSGVFTGMLPFVWFTNQPTNSGMIQSPEIG
                     WGPGNPNLAGFQFNPDYKALIAANPGLFPQTPGVLPTGASLAQVGKDFRLPQIWRSNL
                     GVDVELPWNMVLTGEAIFSKDINAITQININEAAPAGKMTGADNRPYWTDSRVNSTIG
                     TAMELSNTSKGYQLSLTAMLTKNFSHGLSGMFAYTYTLAKDVTANPGSAAASAWVGNT
                     AVGSLNDPGLSYSNFATPHKLVGNISYRIEYAKNLATTVSLVYQGYQTGRWSYIYSND
                     LNGDGNTADLMYIPATADGIQFATYREMTPEAQMNAFWEYVNSNKYLREHKGRYVERY
                     GDLRPWIHRFDLKFLQDIFSNFGTGRRYTLQFSLDILNFGNMLNDSWGTYVYNPLASY
                     DNVRPLAVVTKGTATTAPVYRLNASSLDDFREKTTLSKDISTSSTWGCLLGARLIF"
     gene            complement(27585..28814)
                     /locus_tag="PK288_02810"
     CDS             complement(27585..28814)
                     /locus_tag="PK288_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPM17879.1"
                     /translation="MRLLYNLGILGYGAAAGLMSLFNEKAGQWVNGRKGWKAYLRQKI
                     AAGEKYIWIHCASLGEFEQGRPVIEEFGKSMPEYRILLTFFSPSGYEIRKNYPGADIV
                     CYLPLDTPWNAASFVSIVSPSMAVFVKYEFWNNFIRVLNKKAIPLYLVSGIFRAGQHF
                     FRWYGAFFRKILFRFSHIFVQDERSFKLLRGIGLQNVSLAGDTRFDRVVEIAGSAGEI
                     PLLDNFRGGEKLFLAGSSWKPDEEIIARYVNRDPMKMKWVFAPHEIDESNIARLEALF
                     STTVVRFSRLTKENSGSRVLIIDNIGMLSSAYRYAFIAAIGGGFGKGIHNILEAACWG
                     VPVMFGPNYTNFREAVDLIGRNGAACFRTFEEFSDLADRWLSDDDYYLKSSGTASDYV
                     RQNAGATQVIMSKIRVK"
     gene            29037..29984
                     /locus_tag="PK288_02815"
     CDS             29037..29984
                     /locus_tag="PK288_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPM17880.1"
                     /translation="MVRLIALVISIILQIAAAIIALRFVKLTKYRLSWILLSLSFVFM
                     AVRKIIQFFEFFRGTPSYTWQMIDEWIGVLISFMIIAGVILIRELFFFLKRAEIDRAR
                     AEKRVINAIIITEENERKRFANDLHDGLGPLLSTIKMSMSALGERIKDKPGNTILNNT
                     NHLINEAINTIKDISNNLSPHVLSNFGLSSALSAFTMKINQTRVIEVDFKSNMDKYRL
                     ENEKEVVLYRAACELINNSIRHSGASRIEIELNKHEKFITLQFNDNGRGFDTSSLNQE
                     DGKGMGLSNIDTRVKSIDGVFILESTHGKGTSALIKIIE"
     gene            30008..30649
                     /locus_tag="PK288_02820"
     CDS             30008..30649
                     /locus_tag="PK288_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM17881.1"
                     /translation="MDKIRIIIADDHQLFRNGLKILLNAFPDFEVAGEASNGQEFLNI
                     IKDTRADVALMDINMPEMDGIEATRKGLKQRPDMDIIALSMYGEEEYYYKMVDAGAKG
                     FILKDSDISEVKEAILTVRKGGSYFSQELLYHVIQKIKHRENESKTANLSKREKEILI
                     KICEGLSNQEIAEALFISKRTVDKHRANLLGKTNSKNTASLILYAIKNKLVEI"
     gene            30674..31039
                     /locus_tag="PK288_02825"
     CDS             30674..31039
                     /locus_tag="PK288_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPM17882.1"
                     /translation="MADSHNLGKDGEQQAAELLKKSGYTIRHRNWKSGKTELDIVAEN
                     NDFIVFVEVKTRTSDIFLKAADAVTPQKQKTIIFAADNYIRKYGIEKESRFDIITVTN
                     SGDRSEIEHIENAFYPTLR"
     gene            31146..32186
                     /locus_tag="PK288_02830"
     CDS             31146..32186
                     /locus_tag="PK288_02830"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM17883.1"
                     /translation="MKHGFGIIGAGGIADVHARAIKAINGTGPSGVYDTDRAKCVSFG
                     QKYDCKAFESPDDLCRDPDIDIVCICTPSGHHLEPALMATGEGKHCIIEKPLEITLER
                     CDKIIEAGIRNNVIISGIFPMRFSDVNKELRKAIDAGRFGKFVLGDIYIKWYRSQAYY
                     DEVKWRGSMKDGGGGAVMNQGIHSIDLLQWFMGKVETVCAFTSSPGHADIEIEDTAVA
                     ALKFSNGALGVIEASTAVNPGFFRKIEILGTKGSVVLEEEIIKTWQFSEEEPDDQLIR
                     DRFSGIQTSGGGVSDPKAISDEGHIRQFLDITEAIETNRKPAIDAVEARKAVEIVLAV
                     YDSAANKRIIHL"
     gene            complement(32244..33233)
                     /locus_tag="PK288_02835"
     CDS             complement(32244..33233)
                     /locus_tag="PK288_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPM17884.1"
                     /translation="MKFDQPYRIFSIKNREEFRRTALEIFQYQAANNHVYSLFLKNLG
                     RDYRHITHVEEIPFLPAGLFRSQKIIAENADVEAVFESSTTTGSLPARHYVHDLGIYE
                     RSFLKAFSLFYGDPSRYMISALLPSYLERGNSSLVFMMDRIIRESNHPLSGFYKDDIP
                     RLLASLDKIKAEGRNAILAGVSFALLDLAEKYSPDLSGIIVMETGGMKGRRKEMTREE
                     LHGILKRSFNIGTVHSEYGMTELMSQAYSAGNGIFQSPPWMKIMIRDPRDPLSVIDEE
                     MITGGLNIIDLANVYSCSFIATDDLGRLCGDGGFEVLGRFDGSDIRGCNLMVD"
     gene            complement(33230..33766)
                     /locus_tag="PK288_02840"
     CDS             complement(33230..33766)
                     /locus_tag="PK288_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17885.1"
                     /translation="MRRFLAISVIVLSFFTGCGKEEGSKSSGTATIYNTLYGTGPYYA
                     LGFSFSLGREVSTLGNPKPDITLIADEDIDGTIRRLLLQTENFKNSFYRYGQYSDAAE
                     ASAAFRNLVSASVPAWEESGENIAANQIWIFRTGSEHYAKFRIINTVAEKRSGKAYAE
                     CTFEWVYQPDGTLTFPAE"
BASE COUNT         9277 a         7926 c         7527 g         9188 t
ORIGIN      
        1 acatcataac atattaacaa tcaatcagat aatatttttt gtcatgtact aagattccag
       61 actatcgttt cttcatttaa gattaacccc agatggtacg ccaaatacag taacactatt
      121 gtttacctgg ctcagcaaca gataacaagc atcaggaaca tcggtgaatt cagccgaatg
      181 ctcagcagga tgagcgacca gatgcgggaa gaaaacctcg ccaggtttga agcaaacggc
      241 gaggtttatg acagggtttc ccgtcaattc agtgacaata tcctcggaat tgacaggtac
      301 tacgatccgc atgagggccg tgaagtggaa ctgccaagcg gatacaacta tgcatggtca
      361 aacaataatg gtgagtatat tgttaccgat agccctgacc tgaatccaaa tgtgggatca
      421 aaccttcact gggaactgat gccaaggaaa taacattgtt catccgtcat cctagatggt
      481 tctcattccc ttgtaaacct ttgatctcga caaatcctca aagcggccgg tataggtgag
      541 ctttgcggat gtcacgcccg agatcgttgc cctggccctg aaatccgaaa agaccgtcag
      601 gaagatatca taagaaccca gatttgtaac tatatgaaat ttaacattgt aactcagatt
      661 cttggggttc ttcacgacct ggtaatcacc gatggttcct tcagcggtaa ctccgcccac
      721 accgttatat cctatcctga agtttgaacc cgtctgcaat actccttcat cggcatttac
      781 cataataaaa ttgatcgacg aagaaacagg cactttttca ccatatttgt tttcaaggaa
      841 atcggcttca acaacgaaac tccttgaggt cactagatcg ttcagcaaat tgtaatttgc
      901 gttcagctca gcttccctga gctgtttctt ttccttccgg ctcagcttct cgttcccgaa
      961 atcctgcgaa taaccggtga cggaaatcaa taataatgct gtcagcagga aaagatttcc
     1021 ggtattttta tttattgttt tcattgtttt attgttttat tattcctgcg gtaataaaac
     1081 aaaatatatg ccagtattcg aggaggtcta cctgtgaaag cctgccgaga tgaagtgcaa
     1141 cacctccggc aacgactccc tccagtaagt ccagttgtgt gcaccgtcgc gaaccctgaa
     1201 ttcatggggt atttccttct tccgcatata gatgtgaagc atagcatttc cttcatacag
     1261 gaaatcgtca tccccgcaat cgatatacca gcggacatcc tttttcctgt catcaggtat
     1321 ccggttaatc agggtcggaa tatcatattt ctgaaaataa gcatcatact gctctttttt
     1381 aatctttgag gaatcagcac ccatcgatct cttcacattg gcaagattga cggggccggc
     1441 gccggcactc aggggacagg cagatccgaa aagctcggga tgatgaaggg catagatcag
     1501 tgttccgttg ccacccatcg ataaccctgc aacagcccgg aattccttgt ctttcctgat
     1561 ccgataggtc gactcaatga aaggcatgaa ttcttcaaag aaaaaatcct catacctcca
     1621 ctcattcctt acatcgttgg aataacccct tctgccggta tttgcatccg gcataacgat
     1681 aataacaggg gttgaaaccc catccttaat cgctttgtcg gcaatatgtc tgacttcccc
     1741 gaattgcacc cagcctgtct ggtcatctcc tgctccatga agaagataca gaacaggata
     1801 atccctttga gaagtttcat atcccgcagg caggtagatc gcaaatttcc tttccatttt
     1861 cagtattttg ctttgcatgg taagctggtc ataaaccctg ctttcctggg ctgaaaggag
     1921 cccgccagtg atcatgacaa tcaaaaaaaa tgatagttgt ttcatgtgaa aataaattta
     1981 tatactactg aagattaagt ggttcatatt gaatccacat gaattttatt ttcatgcttg
     2041 tttgtgattt tgtatcatgc gggtttcata acttcctgtt aatagtaaaa ttatctcata
     2101 aaatcaccaa actaataatt tttaccatag cctgaatttg ttttccccgg gcaggacatt
     2161 tataaacggt cctgaaaggt tgccgtccgg aggtcgctta tcaccaaaaa agtcgcggtc
     2221 aatgaccagt acggtcccgt cagggttgtc aaaatcagcc ttcggaattt ttgctttccc
     2281 aagcatttct gtggttactg ttttgacccg gtgactggtg aaagaactgt catatgacaa
     2341 aatgacatat ccgtggccat cttcttcaaa aagattaacc ccctggccgt gagatgggaa
     2401 gaagagaaaa ttttcatcct tctgcgaagg agtcgctccg ttgcaataaa tgttgccgtc
     2461 gatccggacc ggaagtctcg cgttcccgta tcctttaaga ccataatttt cgttttcccc
     2521 tgtcttttca gtgccgggac caatgaaaat attgttgtaa tacctgtcat cgcccccata
     2581 aatggtggta agccctgcca tatcggttga atgcggcagg aaatatggtg tgaacctgtt
     2641 tggttcgggc cagttatgaa cagttccagc aataagatta ttaacgaatg ctccgccctc
     2701 cgactgggtt tttatggccg cacgtgaaag gaatacatta ttgtccacaa tgaaaggtcc
     2761 gtgattgact tcaaggaaaa tatcttccag gtcattgtca tagaaaaggt tggccgaaac
     2821 tcttgttccc tgggtcatcc agtcgagcca caggcccctt ccgcaactgt ggatccggtt
     2881 atttctgatt atggcatcaa ttgccgcatg aaattttatt ccccctattt cggctccggc
     2941 aaactgccgt ttagtccaga tattgtaaat atggttattc tcaattatgc tgaaggctgc
     3001 gcccatgctc ccgcatattc cagtctgttc gcagttgaat ataacattgt tcctaacaat
     3061 atgcgaaccg gtagtttcct tgttccaacc gttccgcagc acccggaaag ttacttcaat
     3121 ataatgtata ttgccatcat tgtaaatatt tcccttgtcg gccgaccaaa cattatgtcc
     3181 cgttccgtgt tctttcccga gagttatgcc cgaacacttt gaatctctta ttacattgtt
     3241 ctcaatgatc caccctttgt tccagtgggt ggcaatcatc ccgacctgtc cggcggttgg
     3301 cgccccccat tgggtagctg cctggcttat aatgaacccc cttattgtaa tgtaattaat
     3361 tcccggcttc ccgggataga tacaggttgg tctgacgctt atctcggtaa gctcccggtt
     3421 cggattgaat ttatggaaat tcgcccagat ggtggtataa tcccggttgc tctcacaata
     3481 ccatgtataa accgatcctt cacgatccat gatcatggtg tcgggaacag gatccagtat
     3541 tttgtccaag gtctcctttt catagaagga tttaccattc agaaatagct ctcccgtatg
     3601 atgtatccgc cccctgtcgt taaaccagtc accaaaaaca gaatcctgat acggattata
     3661 tgatccgaag acagagttgg gtacaacaac tttccagaca ccatttctgt ttaccttagt
     3721 ccagccccga acttcttcgg agcctttgat ttcaacgcgc tcaccggggg cagccctgta
     3781 caaaatcctc ctgctgtcgc tttcccctcc ccttaccggg ttgatccatt ccctgtaagt
     3841 gccggcatgt acggtgacgg tatcacccgg atatgccgcc tgcacggctg catttattgt
     3901 cctgaacggc ttcgcaggcg aaccgtcgta gctgtcattt ccgctaactg acacatggta
     3961 ttcacgcgaa tgcgaaacaa acgcccaaaa caggcttaca aataataaca ggatcctttt
     4021 catcttactc tattattaag ccttctacaa gtttaatcaa taaaaacgga tctggaacat
     4081 cttttttttt ctctttattt caccatctcg tttcactaaa catattctat aaaattattt
     4141 ttataacatt gaggaacgat tgtttcttaa ccaaaccata aaaacacaat aaaatgagac
     4201 caacacggag gaatttcctg aagctttcgg gattggccgg gatcagcctg gcagcatcaa
     4261 atgctctaaa agccattgtt ccgcaaacgg aggatcgcaa atccgggaaa aacagtatcc
     4321 cgggaagttc gtcagaaata tcagcccaaa ctttcaatat gtgcggttat gcagctccaa
     4381 ggctcgataa ggtaaggata gggttcatcg gtcttggagg caggggccgc cttcatgaat
     4441 cgataatgag cattattgag ggcgtagaga taaaggccct gagcgatatt ctgcccgaca
     4501 gggctgcaaa agccagggaa attgccgtga aagccggttt caatcctgca atgtacaccg
     4561 actctcccga tgcatggaaa aaactgtgtg agaggaacga cattgacctt gtttatatat
     4621 gcaccccatg gaatcttcat actccgatgg ctgtgtacgc catggaacat ggcaagcatg
     4681 cagcaattga agtgccggca gccgacaccc tggaagagtg ctggcagctt gtggaaactt
     4741 ccgaaaaaac caggaagcac tgcatgatga tggaaaactg ctgttatgac tttttcgagc
     4801 ttctcaccct gaatatggcc cgccagggat ttttcggcga gcttgttcac tgcgaagggg
     4861 catatattca caacctgctt aaatcgaatt tcaacaagga cggttactgg aacatgtggc
     4921 gtctcaggga gaatgccagc aggaggggca atctctatcc cacgcacggc cttggaccgg
     4981 tctgccagat cctggatata aacaggggcg acaagatgga tctcctggtg gcaatgatga
     5041 gcagggattt tgcaatgaat gaaactgcaa gaaagtttgc agccaccgac agtttcttca
     5101 gaccgtttgt tgacaacccg taccggggta atatgacaac cacaaccatt cttacaaata
     5161 agggtagaac cataatgatc cagcatgatg tcacttctcc gaatgtatat tcaaggatcc
     5221 acaaagtaag cgggacaagg gccgccgcat tgaaatatcc gcttccgcca aagatatctg
     5281 ttgaggatga atgggttacc gatgaggaat tcagaaatat tgaggaaaaa tacactccgc
     5341 cgataatcag gaaagtcggg gagcttgcca aaaaggtagg cggtcacggg ggcatggatt
     5401 ttcttgagga ctggcgcctc atcgactgcc tgcgaaacgg tttgcccctg gatcaggacg
     5461 tttatgatgc tgccctctgg agtgccgttg ccccgctgag cgaatggtca gttgcaaaca
     5521 aatgcaattc tgtagaggtt cccgatttca cacgcggagc atggaagaca aatgctcctg
     5581 ttgacatatc cctaagccag ggaggaaata caggagtaag aggttaaaaa ggacggggca
     5641 tccatcaata gccccattcc ttcattatcc tgagatcttc tttcacacag tccatcgggg
     5701 acataccctg gtaggactcc tgttctatta tgtaaaccct ggtgccacct atccttgtag
     5761 ccaggtcaac aacctcacgg gcattgacaa ttccctttcc gataatggtg ctttcatatt
     5821 tttcaggacc gccggaggaa aggatctcat ctttcacatg aaggttctcg aaccttccgg
     5881 gatactgttt taccacatcc agcgccactg cgcccccatt gtacatgttg ccgatatcaa
     5941 gctgtatgac caccttatca ggatcaatgc tcttcatcat aatgtcgaag agtttttctc
     6001 cccgcagctt ttcactgaac tcaaaatcat gattatgata accaaacttc atgccgtgcc
     6061 gcttgcacag atccccgcac ttgttgaaaa catccatgta tttcctgaaa tcatcataat
     6121 tgttcctcat ggtcatatcc attgaggggc tcacaacata ttcctgccca agtaccgcgg
     6181 catcattgac agtctgcttc cagctgtcgg aaaagtcctt cctggcttca tcccagtgct
     6241 gcttccccat cacggtgtgc ccgcttatca ttttgagccc cagtccgtca agtactttcc
     6301 tgaattctcc cggtgaataa ccatagaact tcccgttcac ataatttgca tgttcaacat
     6361 attcataccc cattttcgca acctgggtga gtgaacccag cggatccctg gccatatcct
     6421 cacgtattga atacaattgc aatccgataa ttccctttgc ctttgatgaa gcaaataatg
     6481 atgacggtct gaccgatgca gctacaacag ccatagcacc tgccctgatg aatgtcctgc
     6541 gtgatgtttt cattaatata ggtttttaaa aacgttcgac tttaagttat tctaaagata
     6601 gaaaatccgg tataaaaaac aaaacaaaca ggtcagcggg cgaactcctg caaaactgaa
     6661 gacttgccgg ctcattccta tgaggagtca gattaatcaa ccggagagat gactgcaata
     6721 accgaaaaat cttcccggga aattgcaagt cccgtttttc agctgattat ctgcccaagg
     6781 tgattttcgc ggctacggcg gctaaatccg gaaaatatgg ttcctgctga ctatgagatt
     6841 gtgttcaggc tggctggatc ttctgtaact gcgaccgtat ccttctccag taaaattcag
     6901 tcatgggtcc ggaaccgttc catgattttg caatcttctc aaaattgtaa gggccgattt
     6961 ttgaggcaaa atgcaaaaac accttcctgg agttctcata atcaaacata tccgacaatc
     7021 tgaaacaact gttggtctgc ctgttgtatt catctattct tacctgccgg atctggaata
     7081 tgcctgcagc tccttcatat tcattaaatg ccattgtatt tcccattgtt tccaccatgc
     7141 cgattgcatg catcaggggc tcaaagggat taatgccccc gggcttgtga atgacaaggt
     7201 aattcctgct tggtgcaaca gattctcctg ctgtggaaaa aagcaggacg agtacaaaca
     7261 aagattttaa tactatggca cttattattc cgggtcttac aaccgctacc ggtttctttt
     7321 ccggggcttc ccttaccata aagatccggt ttaacatcct gagagttaaa tccggaagta
     7381 ttgaaactat gttaattaat tttttcaaaa gtgttgtttt aattttttaa ctgaataatg
     7441 attttttctt aatatatttt agataaaatt ttgtgattaa attatggttt ttcttattcc
     7501 ataaaagtca tgaatttact aagttattaa caatcaccag ttaaaaatca accccggtta
     7561 atatgctatg caaccttata acaacattca tgtaaatatg tttaggcagt cctataacca
     7621 gccttggaga ttttactaaa tcatgttttt ggaataacta actactacag gtatcgtgtt
     7681 cttataataa tcctattaag ttacgagata ataatttcat ttcctaaatt gcgggcggaa
     7741 atttttaatt tatcaccctg agttgcatgc aaattgtttg ttcgtgaaag gaattgacct
     7801 gcatgaattt aaatcacctg aagtttggtc gtccaatatg tggattgcct gcgctgagac
     7861 tcagtatcat ttttccaaac ctcatttcga aaacagaaaa acaaaaaatt cctgtgtgac
     7921 atgtccaaac accaagacat caataaagtt ctcatcatag gatcgggacc gatagtaata
     7981 ggacaggcct gtgaatttga ttactcggga actcaggcct gcaaagcatt aaggtcgtta
     8041 gggtataaga tcgtactggt gaattccaat cccgcaacaa tcatgaccga tccggagatt
     8101 gccgatatta catatatcga gccgctcaac gaatatgcac tgacggaaat cataaaaaag
     8161 gaacgtcccg acgcccttct tccgaacctg ggcggtcaga ccgggctgaa tctctcatct
     8221 ctcctggcaa aaaagggtat tcttaagaaa tacaatgtaa gggttattgg agttaacata
     8281 gatgcaattg aaaggggtga ggacaggatc gcattcaagg agacaatgaa caggctcggc
     8341 ataggaacac ccagaagcaa ggcggtgaac agtgtcgaag ctgctgaaaa tgctgctgcc
     8401 gaacttggtt atccggttgt tgtccgtcct gcctatacaa tgggaggcac aggcggcggt
     8461 ttggtattca atgttgagga actgcggaca attgccacca ggggattgtc agcaagcctt
     8521 gtgaatcagg ttctgatcga agaatccgtt ctgggatggg aggagcttga acttgaagtg
     8581 gtgagggatt cgaaaaacca gatgataaca gtatgcttta ttgaaaatgt ggatgccatg
     8641 ggagtccata ccggtgactc aatctgtact gctcccatgc ttaccatcag cgaggagctg
     8701 cagaaaaggc ttcagaaata tgcctatgat attgttgagg ccatcgaagt gatcggggga
     8761 acgaatgtac agtttgccca tgatccggta acagacaggg tggttgtgat tgagatcaat
     8821 ccacgcacct caaggtcgtc ggcactggct tccaaggcca cagggttccc aattgcattt
     8881 gtttctgcac ttctggccgg cggattaaca ctcgatgaga tcccatactg gaaggaagga
     8941 tcacttgata agtatacccc atcgggtgac tatgtggtag ttaaatttcc gagatggaca
     9001 tttgaaaaat tcgagggcct tgaagacagg ctcggtacac agatgaaggc cgtgggagag
     9061 gttatgagca ttggaaaaac ctataaggag gctttccaga aagcaataag gtcacttgaa
     9121 acaggaaggt acggccttgg atttgcaaag gatctcaacc gcaaatccct agaagaactt
     9181 atgaacatgc tcaatcatcc ttcaagtgaa aggcagttca tcatgtatga agccttgcgc
     9241 aaaggagctg aaatcgacga actttacaag cggactttta ttaaacgctg gttcattcaa
     9301 cagatgaaag agcttgttga ggaagaagaa gagattcttt cctggaaagg acagatgctc
     9361 cccgacgata tgctggtaca agccaagaag aatggttttt ctgacaggta cctttctgcg
     9421 ttgctgggtg taggtgaaaa ggaaataagg acacgcagga tcagccttgg ccttaccggg
     9481 gcatgggaag cggttcccgt gagcggggtt aagaacgcat catattattt ctcaacatat
     9541 aatgcatccg gcaaaatacc ggtgagcagc aaaaaaaaga tcatggttct cggcggagga
     9601 ccaaacagga taggacaggg cattgagttc gactactgtt gcgtacatgc cgcatttgca
     9661 atcagggatg caggtttgga atcgataatg gtaaactgca atcctgaaac agtatccaca
     9721 gattatgata catccgacaa gctgtatttt gagcccctga cagttgagga tgtgctgagc
     9781 atctacaata atgaaaaacc ggaaggcgtc attgtgcagt tcggcggaca gactcccctg
     9841 aaccttgcaa atgagctggc tgaagccggg gtcaggatcc tgggaacaac acctgaaacg
     9901 atagaccttg cagaggaccg cgacaggttc aggcaggtta taaacaggct tggaatcccg
     9961 caaccggaat cgggaatggc aagcaacctg caggaagccc tcaggattgc tgaaagaatc
    10021 ggctatccgc ttatgatcag accttcatat gttcttgggg gacgcgcaat gaaaataatt
    10081 cttgacgagc agatgctcga ggaatatgtg gcaaaagcag ccggagtttc gcccgacaga
    10141 cccctgcttc ttgataaatt cctcgaagat gcagttgaag ctgaagctga tgccatctcc
    10201 gacggggtcg atgccttcgt tccggcaatc atgcagcata ttgaatatgc cgggattcac
    10261 tcgggtgatt cagcctgcgt cataccaccc gtaataatct caaggaagca taaaaaaaca
    10321 atttatgact atacaaaaaa gatagcgacg gagctgaaag tggttggcct gatgaacatt
    10381 cagtatgcca tatatgacga tacggtttat atacttgaag ctaatccgag ggcatcgaga
    10441 actgtccctc tcgtatcgaa agtatgcaat atttcaatgg ccaggatcgc aacccaaatc
    10501 ctcctgggac aaaagctcag ggaattcaat ctgaaaaata aaactttcag gcactatgga
    10561 gtgaaggaag ctgtttttcc gttcaatatg tttccttcaa ccgatcccct gctggggccg
    10621 gagatgcgct caaccggcga ggtattggga atggccgact ctttcggcat ggcctttttc
    10681 aaatcccagg aagctgcaca atcatctttg cctgtaaggg gaacagtgct gatcacaata
    10741 gcagatcgcg acaaacagcg gataattgaa gcagccagga acttcaggga tatgggattc
    10801 agaatacttt caaccacagg aacaaaagcc ttccttgaac aaaacgggat cccggcagaa
    10861 cttgccctga aggttcacca gggcaggccg aatattgtgg atgcaataaa gaacggtgaa
    10921 attgatcttt taatcaatac acctgcagga agactcagtg aatatgatga ctcgtatatc
    10981 aggaaaaatg ccataaaata caaactgccc tatataacca caacttcggc agcccttgcc
    11041 gcaaccaaag ggatcaggga acggcaaaat gaagcttatt tcgtcagatc gctccaggat
    11101 tatcattctt caatcgggga gtaaataaaa agcgggaacc aatcactgaa actccgccgg
    11161 aacataataa cccgtattat tgattccaat tcattgctcc ccgtttcttt cctggtatcc
    11221 ggagtttctt gcagttattc cttcacctgc ttttttttca gttcctgtat ctcattaacc
    11281 ttgtcggaga tctctttttc cttgtcggct acttcctttt ccaatccgga gatcttttca
    11341 ttaagcccgg atatcttttt ttcgagtttg ccgatatctt cctttaattc tgaaatttcc
    11401 ctgccgagct gaactttctc atcttccagc ttccttataa ccggggtata gacggacttg
    11461 gcataaaacc aggctgtaag gtagcctatc aggccggcaa caaccagcaa tgctataatt
    11521 agaattactg ctgcagtaac cgacataatc tgaaatatta ttaatgatcc catgatgtta
    11581 ttttttaatt gaaattactg ttcttctgtt tttcgcacgg tcttcggctt ttatataacc
    11641 ggctacaggc tcatcttcgc ctcttgattc gacaatcatc ctggaagatg gaaagccttt
    11701 tgtctcaaga tatttctgag tagccctggc cctttccatg ccaagcttct ggttgtactc
    11761 ccttgtaccg ataaggtccg tatgcccggt tacaagaagc actgattcgg ggtatttgtc
    11821 cagccagtcc ttgaattcag taagacctga ttcaaactgg ggagtgggtt ttagtttgaa
    11881 tttatcaaac tcaaagcaga tcacaagatc ttcaggcatc atggcattaa tctcgagcaa
    11941 tgagtctgcc acactgattt tcggaactgg gactgcctgc actggttccg gttttcttgc
    12001 agcatgcctt aatacatcag tataaagcca agttgaaaag aatgaccata taacgaatgc
    12061 aatcaatccc gaaataagga ttctcataat ctcacttgtt tggttaatta tcaggtaatt
    12121 atccctacaa gttaggcaat tttaaaagac cagtcaacaa attgactata attattaagt
    12181 tctgtggcga taaaaattat atccgatgtt taaaaattac ggttgaaaaa tgcgatttat
    12241 taatagcttt ggtaaaattt cccttctgag aaaggtgata caggaacttt cagttgcttt
    12301 cgttcagtga gaaatggttt aattcacaac tgattttctg atctttcaaa gaatttcaaa
    12361 taaggttcaa atgaaaaaaa taccgaaatc aattctgttc tgttttttca tctgcctgat
    12421 aaacatcccg gcacaaaccc agtcaggaaa agctgacccg tcgcttgacg aaaaagtaaa
    12481 gaaattcctc aatgaacatg cacggcactg gtatgatatg aatgtcccct gggtggatgg
    12541 tcagctgctt catgacctcg tgctaaaggg aaattacaaa tcagccctcg aaatcgggac
    12601 atcgaccgga cactcggcaa tatggatcgc ctgggcgctc agcaaaaccg gtggcaggct
    12661 catcacgatc gatattgatg agagaaggca ccgaaccgcc cttgaaaact tcagacaggc
    12721 aggactctcg gactatattg atgcaaggct tgccaatgcc catgacctgg ttaaggagct
    12781 gaagggcccg tttgatttta ttttcagcga tgccgacaag gactggtata agaattactt
    12841 tgtcatttta gatcccaaac tcaaggttgg cggttgtttt actgcacata atatttccga
    12901 aagggttcgg ggaagttacg gctcatacag cggcactgcc gtttttctcg attacgtaaa
    12961 gagccttccg aattaccgga ctactgtcaa cagtgcaggc ggtggagtgt caataagtta
    13021 caaaacagcc gagaaataaa taatctgatg atgacgggaa atgcgcttga gagaaggctt
    13081 ggactgttcc ctgccactaa tatcgtcata gccaacatga tcggtgccgg aattttcaca
    13141 acatccgggc tgcttatgtc gggtttgcac gatccagtaa taatggttct gctatgggca
    13201 gccggaggtt tcattgcact gtgcggcgct ttctcatatg gtgaacttgg cgcagcaatg
    13261 cctggagcag gtggcgagta tctttttctt tcgaggcttt accatccgct gttcggattt
    13321 ctcagcggat gggtatcgtt cgtcgtcgga ttttcagcgc caattgcagc ctcagccctg
    13381 ggattcagcg agtatttttc aagggctctc cccggcttta ccctctggat gcaggattcc
    13441 gccatgattc agccggaaat gaccagaaaa atactgtcgg tatctgttat cctggttttt
    13501 acccttatcc attacacagg aatcagatac ggggcccgga tacagaatgt ccttacaatc
    13561 ctgaaaatac tgcttattgt tattttatta acagcagggt tcgcatccgg ccagggcgaa
    13621 tttttcaact tctccagcgg aggaaccatc ccacagggac ttgcaggatg gaaaacaatg
    13681 ggactctccc taatgtggat aatgttcgct tacagcggat ggaatgcttc tacatacctg
    13741 ggcgccgaga tcaggaaccc cgaaacgaca ctgccccgct ccctgatttt cggtaccgga
    13801 atcgtgaccc tcctgtatat cgggataaat atcctctatg tttatgcgat cagccctgct
    13861 gaaatgaaag gagtcatctc cgtgggaggg cttgcaatgg gtaatctttt tggcaaaccg
    13921 gccgaggtac tgttctcggt actgattgcc tttgcattgt tttcatccct cagtgccttt
    13981 attatcatag gtccgcgggt ctattactca atggcaaaag acggcctctt cttcccgttc
    14041 gcagcccgga tacataaaaa gttccatgtg ccgtcaaact cgatcttact ccagggactt
    14101 attgctgtaa tacttgcctt ctcggggact tttgaacagg tccttacata catgggattt
    14161 gcgctgggta ttttcccgct tatggcggta gccggggtct ggaaactgag aagaaacaat
    14221 tccggggcaa tccggctgaa gggatttcct tttacccagg tagtttatct aacggcagga
    14281 tcactgatcc ttatgctttc atttatggaa aggccggtgg aatcatcagt agccattctg
    14341 acggtactgg ctggaattcc cgcctattat cttttccgca aaaattcagg ggcagccggc
    14401 tgagttccgg aatactggcg cggaatcccg gccagatcgc gtatcactac cgaggcgcag
    14461 catatgccaa aagccggtgg catatatgat atcgtaccaa caactgatgc tttattcctt
    14521 tcgccgctaa caggcataac tcgtgactta tcaacaggtt caggcgaata aactgcagta
    14581 aatccgctgt ttataccaag gcggtgaaga cgtttcctga gcatccgggc aagattgcaa
    14641 cctttggtct ccgatatgtc atttatcatt atcctcaaag gatccgacct gccgcccgcc
    14701 cccatggagc ttacaacggg caagcctctt ttcagtgaat tgtatataag ataaaccttg
    14761 ggcgaaaggg tgtcgatggc atccacaaca taatcaaatc cgcgatcaag gatttcacac
    14821 atcctttcat ccctgatata ttcctggatt actgtcaggt caagttccgg atttatctcc
    14881 ctgagcctgt ttcccatcaa tactgcctta ggtgagcctt cggtgctggt aagcgcgatg
    14941 agctgcctgt tccggtttct gggatgtatt gtgtctccgt caactatggt aatactgcct
    15001 gttcctgccc tgcaaatcat ttcagcagca taggctccga cccctccaag gccgacaatc
    15061 agcacgctgg aggctttcag cctgtcaagc ttctcccgac caatcaacag ttcagttgct
    15121 gactgccagt tttccttgta tggtttcatc attattattc aaaataattt tgcagggtaa
    15181 atatattgcc ggttccggct gtttcatccg ccatgaaaaa tttgtgatca gcttctgaca
    15241 cccaggaatt tcttgtaatt gttatacatt gtgattttca gttcatccgt ggatacgccc
    15301 agatcggccg cggccttacg gtagatatcc ctgatgtcgg tatcggcacc atccgtttca
    15361 aggaacagcc ggtcagcagg aataaacctg atcagtccgg ctgattcggg acgcaatgcg
    15421 aactcaaacc agattgataa ataaaatccc cttgaaagca gttgtgaggc aagttccttc
    15481 ttgcccctga atccatggat cagccagttt gctgtgggtt tgagtctttt gtgagccgac
    15541 aggaattcat cccaggcctt tacgcaatgt atcaccatcg gcttgcccag cttttctgcc
    15601 atctccgcct gctcatcaaa cacttcacgc tgaagttcca ttgacggacc cctaagcctg
    15661 tcaaatcccg cttcaccgat tgcaattacc agcgggtgtt cagccacgct tcgcatgaat
    15721 tcagtaagcc tgcttctgtc agcttcattc agatgccagg gatgtattcc tgccgcatag
    15781 gcaattcctc tctgttcaac aggtcggacc gcttcatgag ccataagtac ctccaccctg
    15841 aatattcccg gtgaagagga agctccatgg gtatgaatat caataaagtc gccgggacgg
    15901 ggagaattca tttcttcttg ctgccaatta tggaaattcc cttctctatc cttttaatcg
    15961 tttcctccct gcctatccag cttatgatat cgtatacccg ggggccacga agggctccga
    16021 caatcacgag ccgaaaagca ttcatcacag ttcccgtatt gtatccactg ctttttatcc
    16081 agctgctgac agatgcttcg gtcccggaag gcgaaaaatc agcagtggaa agtagcacgg
    16141 caattaattc cctgagcatc gcgggtgtgc tttcagtcca tcttttgctg acagcttcct
    16201 gatcataatt ctcgggagcc ctgaagaaaa aatctgtctg gtcccacaaa tccttcacaa
    16261 agcttaccct ttccttgacc aggccaatca gtacctcaag cctgacaata tcggtatgat
    16321 aattcctggc cctcagaaac tccctgaatt ccagggccag ctggttgttt gaccttttct
    16381 ggaggtaaac atggttgaac catctggctt tttccgggtc aaatctcata cccgattttc
    16441 cgactctttc aagagtaaac gaattgataa gttcgctcat tgagaatatt tcctggtcag
    16501 tacccggatt ccaccccagg agggccagca tattgacaaa tgcctcaggg taatacccgt
    16561 cttccctgta acctctggcc gtttcaccgt atggccagta gagcggaaaa acaggaaatc
    16621 ccagcttatc gccatcgcgc ttgctgagtt tacccctgcc gtcgggtttg agaagcaagg
    16681 gcagatgtgc aaacaacgga gcttcccagc caaaagccct gtaaagcata acatggagcg
    16741 gaagggacgg aagccattcc tcgcccctga tgacgtgact tatcttcatc aggtaatcat
    16801 cgacgacatt ggcaagatga taggtcggca taccgtccga tttaaacaat accttatcgt
    16861 cgagggtatc tgaatttact actatatgac cccttatcag atcatcaaaa tgtatttcct
    16921 catcccgggg cattttaaac cggatgacga aaggatctcc cctgtcaagc ctggcattcc
    16981 aatcaacggg atcaagtgaa attgaattaa caagcttgtc ccttaccgcc gaattgtata
    17041 taaatgtctc accctttttt tcagattcaa tacgcatcct ttcaagctcc ccgggtgtgt
    17101 caaatgcata atatgcatgt cctctatcga caagcatatc ggcatactgc ctgtacaggt
    17161 gctttcgttc actctgccgg taaggtccga aactgccgcc ttcatttata ccttcatcta
    17221 catctatcct gcaccactcg agtgcccgga tgatgtaatc ctccgctccg tcaacataac
    17281 gcgactggtc ggtgtcttca atccggagga tgaaggtccc gccgtgtttt ctcgcaaaca
    17341 gataattgta gagggctgtt cttacacctc cgatgtgcag aggtcccgtg gggctgggtg
    17401 caaaccgaac ccgtatccca ttcattgtca taaatattca ttttaacctg taaagataac
    17461 taaaccacat aaaataacgg tatcagtaaa atcagctttt acaagacaga aatttactaa
    17521 tttagtaact gccaatcatt cgtgcatgat taaactgctc tgattcctga agtacaggca
    17581 ggaacgctga tttcagatca gggctgttat ttctgaccag taaaaattaa atgacttgca
    17641 gatgaaaaca atgaaagctt tagtaaaggc cttgccggag aagggtttat ggatgcagga
    17701 agtacccatt ccggaacccg gtgtaaacga tgtgatcata aaagtcagaa aatcggcaat
    17761 ctgcggtacg gatcttcata tctacaactg ggatcagtgg gccgccagga caattaaaac
    17821 accgatgatc atcggccatg aatatatggg atacattgaa agtacgggcg caggtgtcag
    17881 gatattcaaa cccggcgaca gggtgactgg cgagggccat cttgcatgcg ggcattgcag
    17941 gaactgcagg cgcgggaaag agcacgtttg cgaaaatact ctgggcattg gggtcaatat
    18001 cgacggttcg ttcgccgaat atgtcagaat cccttcctct aacattgtcc ctctcgatcc
    18061 gaggatcccc gacgactggg cagcaataat ggatcctttc ggcaatgcca cccatactgc
    18121 cctttccttc cccctgctgg gagaggatgt actgataaca ggagccggac tgattggcag
    18181 catggctgta gccattgcaa aattctcagg tgcacggttc attgtggcaa gcgacctgag
    18241 cgattaccgc ctcgaaatag caaaaaaaat gggagctacc ctgactgtca atccatcaaa
    18301 gggagaaaaa atatccgatg ccgtaaaaaa gcttggaatg cggggatttg atgtcggcct
    18361 cgaaatgtcg ggatcgccga aagcatttat tgaaatgatc ggtaacatgt acaacggttc
    18421 aagcatttca cttcttggca ttctgccttc aaatttcgag gtgccctgga gcgacattat
    18481 tttcaaggcc attacactca agggaattta cggaagggaa atgtgggaga catggtacaa
    18541 aatggaacag atgataatcg gcggaattga tctgaacccg gtaataacac atcatttcag
    18601 cattgacgat tttcagaagg gtttcgatgt gatggaagaa ggcaactgcg gcaaagtgat
    18661 acttgactgg gattaatccc tgaaaatgat ttcctttgag ccagttttaa aaaccagatt
    18721 atccctggag gctgccggac agatatttcc cactgaaaca aggaattaca atatgctgaa
    18781 atgatttatc aacggataac accaggattg gcagtcaaac ataaacagga gtatttaaca
    18841 aactgacaaa aacatgtacg agaatttcca ggaacattta aaggccactc ttgatgacat
    18901 cagggaatcg ggcctttaca aaaccgaacg catcatagta tctccccagg gtgccgaaat
    18961 agttctggac acggggcaaa aggtccttaa tttctgtgcc aacaattatc ttggtctgtc
    19021 aaacaacagg ctgcttgtgg aggctgccag gtcagccctt gaaagtcatg gctacgggat
    19081 gtcatcggtt aggttcatct gcggaaccac cgaccttcac aaaaggctgg aaaatatgat
    19141 agcaggattt ttcggaaccg aggataccat tctgtatgca gcctgctttg atgccaacgg
    19201 gggtgttttt gaaccccttc ttaccgaccg ggatgcaata atctcggatg cattgaacca
    19261 tgcttcaatc atcgacgggg ttcgactctg caaagcccag agattccggt atgagaatgc
    19321 cgacatggat gatctcgaga aacagctgaa gctcgcgaga gatctgcgat tcagacttat
    19381 tgtcaccgac ggtgtatttt ccatggatgg caacgttgcg ccgctcgacc gtattttcga
    19441 actggctgaa aggtacgatg ccatgataat ggtcgatgag tcgcactcgg caggggttgt
    19501 gggaaatacc ggacgcgggg tgacggaact tttcaacctc agaggtaaga ttgaaatcat
    19561 aaccgggacc ctcggaaaag cgttcggagg ggcaatagga ggcttcacga ccggcaggaa
    19621 ggagataata gagctcctga gacaaagatc gcgcccctat cttttttcca attccattcc
    19681 tcccctagtt gccgcagcag gcataaaaat gattgaaatg atgaccggaa cgaatgaact
    19741 ccaggaccgg cttcacaaca actcccggca ttttatcagc aggataaccg gcctgggatt
    19801 cgatatcaaa cccacaatgt cagcaatatg tgcagtaatg ctttatgacg caaaactttc
    19861 gcaggatttt gcagccttat tgctggagga gggaatctat gtgaccggtt tttcatatcc
    19921 tgtcgttcct aaaggacagg caaggatacg ggttcagctg tcagcggcac atgaaaccga
    19981 acatattgaa aaagcaatag cagctttcga aaagacagga aggaagcttg gtgtcataaa
    20041 atgaattaat tgcaggaata tccggtatcc gggggaattt acctgccccc ccctttgccc
    20101 cggaattcag acagatgctg aaaacatacc tgatgaacag aattcttttc acaatttacc
    20161 gtattgcagt tcccaaaccg ttacggacca tcctcctgaa aaggaagatc agaaggaaaa
    20221 tccttgatca gtttgaagcc ctccccgaaa ttagcctgaa cgacgaccag agggaggtgg
    20281 taaactatct cagggagaat caggtttcaa ttttccccta tccttttcag aaaaaatacc
    20341 ggcctgagga gataagggtc ttttctgatc cggaccttgg aatgaaatat gtcatccatg
    20401 aaggtaagca actgtatttc aggaggagat ggaatgaaaa aagaatccgg ataagttatt
    20461 ccgagctttt aaaagaacag gacccggaaa gcccgcacag gtatatcaca tcggatttca
    20521 gtccgggtcc cggcgatgtt gttgccgata taggcgcagc cgaaggcaat ttctccctgt
    20581 cggtcattga taaggtaaaa aggatctgta ttttcgagta cgaccgcgga tgggttgaag
    20641 ctttaaacgc cacctttgcc ggctggaaag ggaaagttga aattatcccg aagtatgttg
    20701 ccggatatga tgatgaaagg catatcaggc ttgatacttt tttcatgaca cacaatgaca
    20761 tcacattttt aaagatcgat gttgacggtt atgaaaaaag ggtgctggaa ggctgccgtt
    20821 cgctcctcga atcaaaaatt cccctcagga tcgcaatatg cacctatcat aaaaatgatg
    20881 acgaggcgga atttacccgg ctgctgcagg atcacggatt cagtgtttcg gcatcaaggg
    20941 gatatatgat aaactattat gacaagaaat taaaagcccc ctggctcagg agggggctta
    21001 taagagcagt cagaaactaa tgtcatccgc attttgagga tccgcagtcc ttgcagatca
    21061 ggcagccttc ttcatatacc aggttatccg atccgcattc agcgcattta cccttggctt
    21121 ttgttccgtt tggaatatat tttttcaggg ctctctcaac tccgttcttc cagttgttga
    21181 ttgactcgct gtcgagcttg agcgactgca ccaggttgac cacatcctgg atgggcattt
    21241 cgtgccgcag aacaccggag ataagcttgg cataattcca gaattccggt ttgaacatgt
    21301 gggaaaggcc ttcgatagtt ttcctgtacc cgtatttgtc ggtgtactgg aagtcataac
    21361 gtgtcttgcc gtcatcaagc ttgaccttga ttattttacc cttgattatg gttttcggga
    21421 tagggaatat ctcttcgtcg gcaagaccgg taaatatttc atatggcctg ccctccctga
    21481 gaccgacaaa tgcgacccat ttctcttcgt tgaagttaaa tcttataaca tcacagtcga
    21541 gtgttttcgg gcgcttcggc ctttctgtgc gtatctcgtt ccttccggca atgagcaccc
    21601 cgctgcgtga tccgtcgcga taaacagtgg cccccttgca tcccgatttc caggctgtca
    21661 gatacaactc gctgaccagc tcctctttca catcggtagg aaggttaatt gtaacactta
    21721 tagaatggtc gacccatttc tgaattgcac cctgcatcct gacctttgca atccaatcaa
    21781 catcattagc ggttgccttg tggtaaggtg atttagctat tattgccgat atttcctcat
    21841 cggtcattcc ggttacgaca tcaggatcat acccgttaag cctgagccag tcaacgaatt
    21901 tatgatgaaa gacattgaat tcctcccatg aatcgccaac ctcatccctg aaggtgactt
    21961 ttactccctt gtcattgggg ttgactttcc gcctgcgctt gtaacaggtt gcaaaaacag
    22021 gctcaattcc tgaggtggtc tgggtcataa ggctcgtggt gcctgtcggt gctatggtta
    22081 gaagggcaat atttctgcgt ccgtatttaa ccatgttgct gtacatcaca gggtcggcct
    22141 ctttcattct caggataaaa ggattattct tttccctttc ggcatcgtaa ataaggaaag
    22201 gacctctttc ctttgcaaga tatgtggatg atttgtatgc ttcaagagcg agggttttat
    22261 gaacttccac agagaaatcc gttgcctcat cgctgccgta tttcagtccg agggctgcta
    22321 gcatatctcc ttcggcagtt atcccgatcc ctgttctcct gccttcctcg gatttgttcc
    22381 tgatgttttt ccagaggttc ttttcgacaa gcttcagctc atccgattcc gggtctgatt
    22441 ctattttttc gagtattgag tcgatctttt caagctccag atcaattata tcatccatca
    22501 tcctttgcgc aaggcccaca tgttccctgt acaggtcgaa gttgaatgat gcctctttgg
    22561 tgaaaggctt ttcaacataa ctgtatagat ttattgcaag gagtctgcat gaatcatacg
    22621 ggcaaagcgg aatttcgccg catggattgg ttgaagctgt ggcgtagccc agatccgcgt
    22681 agcaatcggg aacgctttcc ctgatgattg tatcccagaa cagcaccccg ggttcagcag
    22741 atttccaggc attgtgtatt attttattcc acagttctac agcatcaact tccttgataa
    22801 atttcggatt gtctgaattg attggatatt gctgccggaa aggggtttta ttttcgactg
    22861 cccgcatgaa gtcatcggta agctttaccg atacatttgc accggtcact tttccgcttt
    22921 cgagttttgc atctatgaat ttaccgctgt cgggatgctt tatcgatatc gaaagcataa
    22981 gcgcacccct gcggccgtcc tgtgccacct cccgtgttga gttagaatat ctttccatga
    23041 acgggacaac tcctgtcgag gtcagggcgc tgttgaggac aggtgttccc tttggccgta
    23101 tatgcgacaa atcgtgacct acccctcccc tgcgcttcat aagctgaacc tgttcctggt
    23161 caatcttcat tatgcctccg taggagtctg attctccttc gttgcctatt acaaaacaat
    23221 tagacaggga ggcaatctga aaattattgc ctataccagt catcggaccg ccctggggaa
    23281 ctatgtatct gaaatcctta agaacattgt agataagttc ctcaggaaga ggattcttgt
    23341 atttcttttc tatcctgtgt atctcccttg cgagccttct gtgcatatca tcgggagttt
    23401 tttcataaag gttcccgaaa gaatccttca atgcatattt attggcccag acccttgcag
    23461 caagctcatc cccgttaaaa tacctgatac tggcttcaac tgcctcatca tgagtgtatt
    23521 taaccattac agctttcttt tctgataact tttccttgtc ctttgccacg tcattttttc
    23581 ttggttctgt aacaggctct tcttttaact tttcggatac tactgctggc tcccggataa
    23641 ataactctcc cataatagat aataaaattc ttttaacctt tgattctgtt gcgattaaat
    23701 gtccactgat tttttaagcg ggtattcgct aaattagaca agcataattc tcgaagcaag
    23761 acaatatttt taactttatc aacatttggc gagggagtta ttaacttcgc aattgtacgc
    23821 tgattgtgag atacttacgt agaaagcctt ccttcgctcg ctaaaaacct ttatatatct
    23881 gaaggttacc ggtgaggtat tccggcaggg gctgaaacca gcatctgaac agtcagggtc
    23941 tggacggtat cacatggtcg tacccggaat ttcattgaaa gggaaaaaag aggaagagaa
    24001 agaagtaaac caataccggc ttttataaaa aagagtgcaa aggctgagtc tcacaaactc
    24061 cctttgcact catctcatat ttatttcccg cctttccggc gatccgggaa tttatatatt
    24121 gtcttcttca ggcaaccggg tcctgctcag aaaataagtc ttgctccgag aaggcagccc
    24181 catgtgctgg aggtgcttat atccttcgac agggttgttt tttccctgaa gtcatcaagt
    24241 gaacttgcat taagcctgta gacaggtgcc gttgttgctg taccctttgt taccacggcc
    24301 aggggccgga cattgtcata gcttgcaaga ggattataaa catacgtccc ccatgaatcg
    24361 ttcaacatgt ttccaaaatt cagaatatca aggctgaact gcagggtata tctcctgccg
    24421 gtgccaaaat tggaaaagat atcctgaaga aatttgaggt cgaaacgatg gatccagggc
    24481 ctcaggtcgc catacctttc aacgtaacgt cccttgtgtt cccttaaata cttgttgctg
    24541 ttcacatatt cccagaaagc gttcatctgt gcttcaggag tcatttccct gtaagttgcg
    24601 aattggatcc cgtcggccgt tgccgggatg tacatcaggt cggccgtatt tccgtcgccg
    24661 ttcaggtcat tggaataaat atacgaccac ctgccggtct gatatccctg ataaaccagc
    24721 gacaccgtgg ttgcaagatt tttggcatat tcgatcctgt aggaaatatt tcccacaagc
    24781 ttatggggag tggcaaagtt agagtaacta agccccggat cgttaaggct gcccactgca
    24841 gtatttccga cccaggccga ggcggcagca gaaccgggat tggcagttac gtccttggca
    24901 agggtataag tataggcaaa cattcctgaa aggccgtgac tgaagttttt agtcagcatg
    24961 gccgttaatg acagctgata ccccttgctg gtatttgata gctccatggc tgttccgatc
    25021 gtgctgttca ccctggaatc ggtccagtag ggcctgttat cggcacctgt catttttcct
    25081 gccggggctg cctcattgat attgatctgc gtaattgcat tgatatcctt tgagaatatt
    25141 gcctctccgg tcaacaccat attccatgga agttcaacat ccacacccag gttcgatctc
    25201 cagatctggg gcaacctgaa atccttcccc acctgtgcaa gtgatgcacc ggtcggaaga
    25261 acaccgggag tctgagggaa caaaccgggg ttggcggcaa tcagagcctt gtaatcaggg
    25321 ttgaactgaa atccggcaag attgggatta cccggccccc agccgatttc gggagactgg
    25381 atcattcccg aattggtcgg ctggtttgta aaccacacaa aaggaagcat acccgtgaat
    25441 actcccgaac cacccctgat ctgtagtgaa cggtcgccca tcacatcata gttgaatcca
    25501 aaacggggag aaagcagcgg tttgacttca ggccacgctc ccacgtccat tctacggcca
    25561 tctataaagg taagtgctga aattgccgga ttattatcca gtttattgag gtacaccggg
    25621 agctccacgc ggaaacccaa tgttgttttc agttttggtg ttacctgcca ttcatcctgg
    25681 agatagaccg cggccatacc gaaagtaagt tcagcacccg gtgcatcatt tcccttataa
    25741 ccataggtaa ccccgaaacc tgtcgggtcc tcaccgttaa tgaacgcatc aaccgaagga
    25801 taacggtaat aactcgtccc ttcacgtatg taggaattat aaaaatacag gttgtcgtag
    25861 gcaagtccgg aagtgacggt atgcttattc aggttaatgg tgacattgtc agtcaggctt
    25921 agtgttttgt tggctaccct gttattgtag gtgaacagtt catatccgaa cgacatgtat
    25981 tggtctccat cctgccagat atcgacaaac gggaaaaatt ttgaagggct tgtacgctta
    26041 tcctcaatat aagtgtagct tgcaagaaac ttgtttgaaa ctttcggccc gagggtgctg
    26101 ttgagttctc ccgtgactga acggactgtg ttcctgaatc catagaagga atttgaaaat
    26161 gcaagtgact ggttgcttat tcttcccgat cccctggaat tatttggcgg accactggtg
    26221 gcattggtct ctgaatcgga tgttcctatt acgtcattgt aacgtaatgt aaacttgtga
    26281 ttcttgttaa tgttccagtc gagtcttaca aggatctttg tattcttgtt catgaaagga
    26341 tcaaagttct tgtattttcc agggtcataa ttatagtttg acatcaggta gttccttacc
    26401 atcgccatat ctgattcccg tgtacgggag attttcagat ttgagtttgc aaccccgtcc
    26461 tggctcggat tccagcttac cccgggtata atttcctttt catattcacc gcttacaaag
    26521 aagaaaagct tattcttaat caccgggccg cctaccctga atccatagtt ctgaagctgc
    26581 ctggtattgg cattggcaac ttcattgccg tcaactgtat tcccggtaaa tgatttcgga
    26641 cgctggtagg tgtatactga agccttgaaa gtattgtcgc cgctgcgggt aactgcattt
    26701 atgcttgcac cggtaaactg tgacatcctc acatcgtagg gagcgacatt tactgaaatt
    26761 tcctcaagcg catcaaggga tataggctgg gcactgcctc cggggagagg attgctgctt
    26821 aatccgaagt tattgttgaa ggcagaacca tcaaccgtta tggtattgaa gcgcccgtcg
    26881 cgaccagcta tcgagttacc ctgcgattgc ggggtatatt ttgaaaaatc ggtgatactc
    26941 ctgttgatcg agggcaggtt tgccagatcg cggctgctga catttgtctg ggtgccggtc
    27001 cgctccgaac tcaggatgga atttcgaagc cctgacgtaa caacaacttc ctgcaatgtt
    27061 gttgtcgctt cggaaagttc cgcattctga acataacttt cacccagcct gagatatata
    27121 tcagtataat tgctggtgga aaagccgata aaggttaccc tgattgaata ggggccgcca
    27181 acacgcatgt tctgtatcct gtagttaccg gcattgtcgg caacggttgc atactgtgtc
    27241 cctgatggta catgaacagc aacaatggct gcgcccggca gaggctggcc ggagccgtct
    27301 gttacccggc cgttaagtcc ggatgtcgtc gccccctgac catatagaaa tgcgggtatc
    27361 atcaggaaga ggacaacctg aaaaactgtt tttataatcc ttttcataaa tcgagattta
    27421 aggtttggaa ataacattac aagactactg aattttatta tcttactgat ctgatattta
    27481 attaaatatt gttaaacaca tagtcgaaat acgatttttg cgggcaatgt ttttcaaatg
    27541 gtgccggcat gatgattaaa caagcaggaa acgttattgt taatttattt taccctgatt
    27601 tttgacatta taacctgtgt cgcacctgca ttctgcctga cataatcaga tgcagttccc
    27661 gacgacttca gataataatc atcatccgac agccatctgt cagctaaatc cgaaaattcc
    27721 tcaaaggtcc ggaagcaggc tgccccgttc ctgccgatca gatcaacagc ctccctgaaa
    27781 tttgtgtaat tgggaccaaa catcaccgga actccccagc aggccgcctc gagaatatta
    27841 tgaatacctt ttccgaatcc gcctccgatc gctgcaatga aagcatatct gtatgctgaa
    27901 gacaacatac ctatattatc tattatcaat accctggaac ctgaattttc tttcgtcagc
    27961 cttgaaaatc tcacaacagt tgtactgaat aaagcttcaa gacgggcaat atttgattca
    28021 tcaatctcat ggggagcaaa aacccacttc attttcatgg gatcccggtt gacatacctt
    28081 gcgattatct cttcatctgg tttccaactg ctgcctgcca gaaacaattt ctcacctccc
    28141 ctgaaattat ccagcagagg tatctccccg gctgaaccag cgatttccac gaccctgtcg
    28201 aacctggtat cacctgcaag ggaaacattc tgaagaccaa taccccgcag taacttgaac
    28261 gatctttcat cctgtacaaa aatatgggaa aatctgaaaa ggatctttct gaaaaatgct
    28321 ccgtaccatc tgaaaaaatg ctgaccggcc ctgaatattc ccgacacgag gtaaagggga
    28381 atagctttct tgttcagtac gcggatgaag ttgttccaga actcgtactt tacaaaaact
    28441 gccatcgaag gagaaacgat cgatacaaag gaagctgcat tccacggagt gtccaggggc
    28501 agatagcata caatatccgc tccggggtaa ttctttctta tctcatatcc cgaaggagag
    28561 aaaaaggtca gaaggatcct gtattcaggc atgctctttc cgaactcctc aattaccggt
    28621 ctaccctgtt caaactcacc cagtgatgca caatgaatcc agatatactt ttcacccgca
    28681 gctatcttct gcctcagata agctttccag cccttcctgc cattaaccca ttgccctgcc
    28741 ttctcattaa ataatgacat caatccggca gctgctccat atccgagaat acccagatta
    28801 tataataagc gcataactta aattaaacag taaaggtaat aaaaaggtgg ttctacaaag
    28861 ctgattcaaa tcatagcagt ttaaatcctg aattatattc ccggtgagta atggcccatg
    28921 gtaattcctg agacaactgc atggatcaga atgctttttt ccgggcatgc ctgatactga
    28981 catgcacaaa tttaaaatta cttctacttt tgtgtaataa tcgataatta taagcaatgg
    29041 taagacttat agcactggta atctcaataa ttctgcagat agcagctgca ataattgccc
    29101 tgagattcgt aaagcttaca aaataccgac tttcctggat tcttctttcc ctgtcgttcg
    29161 tatttatggc cgtaaggaaa atcattcagt ttttcgagtt tttcagaggt acaccatcat
    29221 atacctggca gatgattgac gaatggattg gtgtgctgat ctcttttatg ataattgccg
    29281 gtgttattct tatccgtgaa ttgttttttt tcctgaagcg tgccgagatt gaccgagcaa
    29341 gggctgagaa aagggttata aatgcaatta ttatcaccga ggaaaatgaa aggaagcgtt
    29401 ttgcaaacga cctccacgac ggtctgggcc cattgctttc caccataaaa atgtcgatgt
    29461 cggccctggg cgaaaggatt aaggacaaac ccggaaatac cattttgaac aacaccaacc
    29521 atctgataaa tgaagctatc aatacgataa aggacatctc gaacaacctg agtccccatg
    29581 ttctttcaaa tttcggactg tcaagcgccc tcagtgcttt cacaatgaaa ataaaccaga
    29641 ccagggtgat tgaagtcgat ttcaaatcaa acatggacaa ataccggctt gaaaatgaga
    29701 aggaagtggt gctctaccgg gccgcatgtg aacttataaa taattctatc cgtcattcgg
    29761 gtgcctcaag gatagaaatc gagctgaaca aacatgaaaa atttattaca ttgcagttta
    29821 atgacaatgg ccggggtttc gataccagct ccttaaatca ggaagacggg aagggtatgg
    29881 ggctgtcgaa catagacacc cgtgtcaaat cgatcgatgg agtttttatt cttgaaagca
    29941 cacacggaaa aggcaccagc gctcttatca agataattga ataaggctgc acataagaac
    30001 ttaatacatg gacaagatca gaattatcat tgcggatgac catcagctgt tcaggaacgg
    30061 tttgaagatc cttttaaatg cctttcccga tttcgaggtt gccggagaag cttcaaacgg
    30121 ccaggaattc ctcaatatca taaaagacac ccgggccgat gttgccctga tggatatcaa
    30181 catgcccgaa atggatggta tcgaagccac caggaaaggg ctgaagcagc gtcccgacat
    30241 ggatataata gccctttcaa tgtacggcga agaagagtat tattacaaaa tggtggatgc
    30301 gggggcaaaa ggcttcatac tcaaggattc cgatatttcc gaggtcaagg aagccatcct
    30361 caccgtaaga aagggaggca gttatttctc acaggaactt ctttaccatg ttatccagaa
    30421 aatcaagcat cgtgagaacg agagcaaaac tgccaatctt tcaaaaaggg aaaaagagat
    30481 ccttattaaa atatgcgagg gactttcaaa ccaggagata gcagaggctc ttttcatcag
    30541 caaacggaca gtcgataaac acagggcaaa tcttcttgga aaaaccaatt cgaagaatac
    30601 tgccagcctg attctttatg ccataaagaa caagcttgtt gaaatataaa taaaaagcta
    30661 aaaaagtcct gtaatggcag actcacacaa cctgggaaaa gacggcgaac agcaggcggc
    30721 agaacttctg aaaaaatcag gatataccat acggcaccgc aactggaagt caggtaaaac
    30781 tgagcttgac attgttgccg agaacaatga tttcattgta tttgtggagg ttaaaacacg
    30841 tacaagcgat atttttctca aagcggccga tgctgtcacc ccgcagaagc aaaaaacgat
    30901 aatattcgcc gccgacaact atatcaggaa atacggtatc gaaaaagaga gccgtttcga
    30961 tataataaca gttacgaaca gtggagacag atctgagata gaacatattg aaaatgcatt
    31021 ttatcctacc ctgcgatagg ctggcaaaat gtttcatcac tgaccgtgat tcagtcaggg
    31081 ataataaaaa atgacaaatc cccgatgagg ttcaactgat ttcagggctt ttaactaaca
    31141 tgcaaatgaa acatggtttt ggtataattg gtgccggcgg gattgctgat gtccacgcca
    31201 gggccataaa ggcaataaac ggtaccggcc cttcaggggt atatgatacc gatcgggcaa
    31261 aatgcgtatc attcggacaa aaatatgact gcaaggcgtt tgaaagccct gatgatcttt
    31321 gccgtgatcc tgacatcgat atcgtatgta tatgcacgcc ttcgggtcat catctcgaac
    31381 cagcgctcat ggcaacaggg gaagggaaac actgtattat tgagaagccc cttgaaataa
    31441 ccctggaacg ttgtgacaag atcattgaag cggggatcag gaacaatgtc attatctcgg
    31501 gtatttttcc catgcgattc tcagatgtga ataaggaact gcggaaggca atcgacgccg
    31561 gacgattcgg caaatttgtg cttggagaca tatatattaa atggtacagg agccaggctt
    31621 actacgatga ggtaaaatgg cgcggcagca tgaaggatgg gggcgggggt gccgttatga
    31681 accagggcat acattccatc gatctgcttc aatggttcat gggtaaagtg gaaacggtat
    31741 gtgcatttac ctcctccccg gggcatgcgg atatagagat cgaagacact gcagttgcag
    31801 ccctgaaatt cagcaacgga gctctcggtg ttatagaggc ctcaacggca gtaaatccgg
    31861 gattcttcag aaagattgaa atacttggaa caaagggctc cgttgttctg gaagaggaaa
    31921 ttatcaaaac ctggcagttt tctgaagaag aaccagacga tcagctgata agagatcgct
    31981 tttcggggat tcagacctct ggcggaggag tatccgatcc caaagcgatc agcgacgaag
    32041 gacacatcag acaattcctc gatatcaccg aagcaattga aacaaaccgc aaacccgcta
    32101 ttgatgccgt tgaggcccgg aaagcagttg aaattgttct ggctgtttac gatagtgcag
    32161 ccaataaaag gatcattcat ttataacctg ttgacagcag cctgacaagg taagtttagt
    32221 gttaatttat tcacaatcag ccgttaatct accatcaggt tgcagcctcg tatgtcactc
    32281 ccatcgaacc ttcccagtac ctcgaagcct ccgtctccgc agagccgtcc aagatcgtcc
    32341 gttgcaataa aggaacacga atacacattt gcaagatcaa taatattaag tccgccagtg
    32401 atcatttctt catcaatgac ggaaagcgga tcacggggat cccttatcat gatcttcatc
    32461 cagggaggcg actggaaaat accattaccc gccgaataag cctggctcat caactcagtc
    32521 attccatatt ccgaatgaac cgtaccgata ttgaaactgc gcttcagtat cccgtgaagt
    32581 tcttccctgg tcatctcttt tcttcttccc ttcattccgc cggtttccat gacaattata
    32641 cccgacagat cgggagaata tttctctgcg agatcaagca gggcaaaact gactccggca
    32701 agtattgcgt tccggccttc agccttgatc ttatcaaggc tcgccagcaa ccggggtata
    32761 tcatccttgt aaaagccgct cagcggatga ttgctttctc ttatgattct gtccatcata
    32821 aagacaagcg acgaattgcc ccgttcaaga tacgatggga gaagtgcaga tatcatgtat
    32881 cgggaagggt ctccgtaaaa caggctgaaa gctttcaaga agcttctttc atatatgccg
    32941 agatcatgca cataatgtcg tgcaggcaat gatcctgttg tggtactgct ttcaaaaacc
    33001 gcttcaacat ctgcattttc agcgataatt ttctggctgc gaaaaagccc tgcaggcaaa
    33061 aagggaattt cttccacatg cgtgatatgc ctgtaatccc ttcccagatt tttcaggaaa
    33121 aggctatata catgattatt tgctgcctga tattgaaata tttccagggc cgtacgacgg
    33181 aattcctccc tgttttttat actgaagatc ctataaggtt gatcaaattt cattcagcgg
    33241 ggaaagtaag ggtcccgtca ggctgatata cccattcaaa agtacattca gcataagctt
    33301 ttccactccg cttttcggca actgtgttta tgatcctgaa ctttgcataa tgctccgagc
    33361 ctgtcctgaa gatccagatc tgatttgctg caatattttc ccccgactct tcccatgcag
    33421 ggacggaagc tgagacgaga ttccggaaag cagccgatgc ttcggctgca tctgaatact
    33481 gcccatacct gtaaaaggaa tttttaaaat tttcagtctg aagcagaagc ctgcggatcg
    33541 ttccatcaat gtcttcatca gcaataagag tgatatcagg tttgggattg ccaagggtcg
    33601 atacctccct gcccagggag aatgaaaaac ccagagcata atagggccct gtaccatata
    33661 gggtattata tatggtggcg gtcccggaac tttttgatcc ttcttccttc ccgcatcccg
    33721 taaaaaaaga cagaacgatc actgatattg ccagaaacct tctcataatt attgcaaata
    33781 taacccaaac aaaagacttg gcggccacta atttccggga atatcaaatc cataatttat
    33841 gggtcgcaca attcgctgca cttataaaag catatcagcc cggttgaatg gcactgacac
    33901 ataccgtacc atgactgg
//
LOCUS       DAOOYG010000016        33453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1101_length_33453_cov_7.346817, whole genome shotgun sequence.
ACCESSION   DAOOYG010000016 DAOOYG010000000
VERSION     DAOOYG010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33453
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1101_length_33453_cov_7.346817"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PK288_02845"
     CDS             complement(<1..548)
                     /locus_tag="PK288_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPM17886.1"
                     /translation="MVFFCFLISCGTSAERKEGFEFRQNEQGLDLYESGDPVFHYQRE
                     PGVIGNQYICNNYLHPLYSLDGDTLTEEAPEDHPYHRGIYWAWHQIYINDQSIADGWI
                     MENISQEVIRLTTWTDESTAVLEADVLWKSPLWHDRSPFVREHTVITTHRKEELLRII
                     DFEIGLRALTNGVSIGGSDDEKG"
     gene            complement(1105..3306)
                     /locus_tag="PK288_02850"
     CDS             complement(1105..3306)
                     /locus_tag="PK288_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17887.1"
                     /translation="MKKVHLMLVGLLLCPQLLPQYSPVAHRYLTPGEIPVSSPGSYAG
                     DGRRFVLVNDISAEGSALFLGNNVTLDLNGYTLRYAAGGYEHIPNSGFEEGSSGWDLS
                     KAPGAEIRNTEEVHVFMGSKLMSLQPGDEIVSQYVNLPVAGRSYFAMCGITGRYYHDM
                     KKYPDDEMKVSIFVEDAQGRNVVCSTKYGDGTRISCPVEKKSPRLGGGFVFAHLHGLP
                     AGKYRVRIKADTDCLVDEIDIRPAMDAGISIIGNTTPLAHYDHIIAESYPPVTPAFYD
                     FTGDFERNLPLPSLPHISGSGVITIKNGIIEAGTPGIQSWGIQSSAPDVMVVLENVKI
                     VTAGISCGSADILQTKIKNCRFEADVPFLIQRHVNHCSVLIRGTNPSEVSNSEFFGGQ
                     GCLSVTGKYSVVHDNLFVNDQWVTNHYSIMGTGDSSKIFNNRFEPKQGSGIYVSRYTE
                     VFNNLFKIETSKPTCEYGREEYSTAAIRLGDYNAAPGSRNASAGNRIHDNRIFITARD
                     FGPAEYIPMCWGIYYSASGGENYVYDNEFAVEKTDPSSRVITAALYICGGPRYYGGQF
                     YGNVIRTNVPAAWIASMYGGAANSDIYCNTVIATGNDPFKTFRMGSSGCDDCVAKLIK
                     FRSNFVRGQEFGIDATDQDHSFTVYWTLNAVLMDKPGRPGKNRDVTIFDNTGKIAVQG
                     KTNNQGKFSAELPEYQANGKDRIFFTPYTIVSGSSSITTDLNRNTDVILTY"
     gene            3769..5616
                     /locus_tag="PK288_02855"
     CDS             3769..5616
                     /locus_tag="PK288_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPM17888.1"
                     /translation="MTLLTAAVLYAASSQVLYAQEDMMHFSKVFNRAKPYRIFLPADY
                     HKLDRHYPVIYYFHGNTGDHKLDIPGISGLVNANGVILVAWNGRSEESDLRPYNIGNH
                     SNIKYNIQFRDYFPELVDYIDKTYRTIPDRSARGVIGHSMGGIMSFFLAGQYPDLICA
                     AFSSKGSPEFFIGTPARHTLYQVRNMFKNLIGIKTGFATSTECELYYLNNEVINGALN
                     EKDLDFRLFRYEGSHDITPKQFSDAFEFVAEAFRNPEPAPERWHHSDIYPDFRIWGYE
                     VESNLNEPGFIDMKGVTRGGFGISTKSWEPDGKPLKGVNIRVKTAPLYSPNTVYTILD
                     YSHSTGSAKICQLTSDKSGRITVDTDNENHQIGIFRKKDPPEIVFIKHNVNDKGSFLD
                     QKDENSLNLTFLNRGGSKAEKVLVTLSTDVPGVSIANPVIELGSLPSCELKALPQGFK
                     ITARNKPPSDGSPFMIRFNLAIRDGKNRVWKDEFYVPVFFDVPEFTHLGIDDGDSEVF
                     GSGNGNNIAEPGETVMIYEISNGSKRLRLYYDDPYIDGERLYDEIQPDKWGDGYSLSS
                     LIHISDVCPPGHKIRFLACYEVKDWQKIRRDVTWGTFTLTVGGELHGKQ"
     gene            5640..7553
                     /locus_tag="PK288_02860"
     CDS             5640..7553
                     /locus_tag="PK288_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17889.1"
                     /translation="MQKLKNKRPERILILFALAIISMSVHVTKASVPADPAAKRADDR
                     GKVFIVNAPIRDIAEFRKLVIQAARLKPYGEVQVNISALAGGGFHEVPEGNNFWHQYA
                     SYNATLFKFFPDPAIAPFIPADFVKRNREYLLARARILREYGINAEFWCNEPNFLPDA
                     FFDRYPGLRGPRVDHPRRGNHPAFSLCMDLEQSRKMVAGMIEELLKNVPEIRTFVFKT
                     NDAGSGICWSDWQYSGPNGPSHCRGISMGRRVASLMELFNEGAKKTGNELTIYLAESM
                     FSDEEMTDILKYLPANCHALGTDSDNSISIGSNISGYYPVRGLLDPVDLLRKIDQMKD
                     DRYKTVFINFKSYYDRGYELMPVIEKVIDLITDNMGQPAVEREIARMLQVEDLCRKWA
                     GDRESKKLFDAIMLLEDAKKYKNSAVGNVTGIYWGVSTRHINRPLVFAPQLLTREEEA
                     YFLPYVFNPSTEEARMDYTDIHGSHNSVPSGVIPRYVSMIVRAAGLMEGIGKDIPESM
                     FIQTWARALRLHAGIMRSAGNFAEAQAIRDRNADKLSRPPHRPGKEPTWDGDPDLQAF
                     NTVMRDELDNAGELIELLSGGGTDILCHASDPAQEDTFLLGPGLIRQLKLKQKIMLAH
                     WTDIEGYLATPFK"
     gene            7558..8541
                     /locus_tag="PK288_02865"
     CDS             7558..8541
                     /locus_tag="PK288_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17890.1"
                     /translation="MKINIFAASNNHVEMKTRLILAAVLIILISSSCGQRKRGDKTAV
                     KGDPFPKEMVEFVPYENNPVFTGTGQDTWDRTIRERGFILNENGTYKLWYTGYNGPDT
                     VTKYLGYATSPDGINWTRYEGNPVYNKRWTEDMFVFREDNNYVMYAEGRNDVAHILIS
                     EDGINWQDQGDIKILTTKGDTIPGPYGTPTVWHENNLWYLFYERNDDAIWLAVSNNQK
                     TWVNFQDEPVLERGPENYDSGAVAVNQILKFRGRYYMFYHATSDPSWIEPGNSAVWTS
                     NVAMSEDLYHWTKYPGNPIVEGDHSSPVLVPDGDKFRLYTMHPGIWLYFSK"
     gene            complement(8641..9789)
                     /locus_tag="PK288_02870"
     CDS             complement(8641..9789)
                     /locus_tag="PK288_02870"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPM17891.1"
                     /translation="MISQPKSLPVSRLTGFPALLLLCLALGSCNRINEKTTVNYVITG
                     KPVIASMNSDDKWTRCFRNFDGSVYFKNFISHDAGRSLSELTDTVFRDVNGAPERAVF
                     VKDGLFYALDGPTSFIEPGVYKGKAWRSTDNLKTITTEEPVFNIPGGCMPDNGIDNWY
                     GIFVYRTILEISPDLWLMTMYGNMETDTVLPSNRDAMKELEYMMRTIIVKSGDKGRTW
                     NYLSTVAVPHAGEPVGEGFVEPAITKLNDGRLLCVMRSGHHFPLYSSWSADTGKTWTP
                     PLYTGFDRGCDPCLITLHDGRIALSWGKRFPEGWSIISDAGDKGLFEYPGKGYTSLSI
                     SNDGGQTWKTDKIIRNAGSCYTTIFEIEPDIIFMQCDKWYCRIRLKKN"
     gene            complement(9881..11155)
                     /locus_tag="PK288_02875"
     CDS             complement(9881..11155)
                     /locus_tag="PK288_02875"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPM17892.1"
                     /translation="MEKNKDITKGSMEEVFRKVLKAGKTLSSLDQSAIRDVILKVAER
                     AIESTDMILEANRKDLDLMEKSDPKYDRLLLSPERIKNIAGDMRNVASLPDPLGRILS
                     EEVRPNGLIVKRVSVPFGVVGVIYEARPNVTFDVFSLCFKSGNACILKGGSDAINSNT
                     AIISIIRKVLAENGIDENAAFLLPAGREETIQLLNARDYVDLIIPRGGRDLIDYVRNN
                     SSIPVIETGAGVCHTYFDEFGNRETGRAVVHNAKTRRVSVCNALDCLIIHEKRLDDLG
                     YIVELCAGNNVVIYADEKAYRALEGRYPAKLLKKAEKDSFGTEFLSYKMAVKTAGSFE
                     EAVAHIDEFGSKHSEAIISENSERINRFFKLVDASSVYSNASTAFTDGAEFGLGAEIG
                     IATQKLHARGPMALNELTTYKWIIEGNGQIRK"
     gene            complement(11190..12305)
                     /gene="proB"
                     /locus_tag="PK288_02880"
     CDS             complement(11190..12305)
                     /gene="proB"
                     /locus_tag="PK288_02880"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPM17893.1"
                     /translation="METELSKFERITVKVGSNVITQKDGSLNVSRILRIVEDISVLSK
                     QGKKVILVTSGAVAAGRSEVVPSKRTNIVGAKQIWASIGQVKLMANYQFLFGKYGMQV
                     GQLLATKESFMDRRHYLNMKNCITAMLDSNVLPILNENDSISIDELMFTDNDELSGLI
                     SAMMDCHTLFLLTNVDGIYNSEPNSPGAELIREVYPDSHNVSGGISAIKSGFGRGGML
                     SKYSTARKISNLGIDVYIANGNRDSIITDIIRGRDVPFTHFHASVNKKSGVKKWLNHS
                     DTFAKGAVFINNGAKAAILSEKASLLFIGITGVEGYFQSGDIVRVLDEEGKYIGLGQT
                     KFDSEKTTKLIGQKHSKPFIHCDFLVISQEIKGNVTV"
     gene            complement(12564..15320)
                     /locus_tag="PK288_02885"
     CDS             complement(12564..15320)
                     /locus_tag="PK288_02885"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPM17894.1"
                     /translation="MRFIFLFLLCLLQYCAGPDCGITPANLTCEYLRDPPVTDVPEPR
                     LSWVNEQGPDARGQLQTAWEIRVAGSPGLLRSGRADLWNSGKVVSDQSCNVRYKGKPL
                     KSRQTCYWQVRVWDRDGNVSAWSNPASWSMGILDQCEWKAKWIGAPWQGEEPLPKPSG
                     PGAKLPDLLPPPAPEFRKEFEVSKQVANAVAYVTGLGYFELYLNGKKVGDDVLVPNQT
                     NYGKRPYLNKENIPLEDNFKGYKVFYLAYDVTGSLRQGKNALGALVGNGFYNPAKYWA
                     GGYGTPRLILQMHISYTDGSEDVILTDETWKVARSPILMDMVFYGEHYDARLEQDGWC
                     EPGFNDSGWENAVLRKAPEGALRAHMAYPDRVMEILKPVKLEKLSEGRFKVDFGQEIS
                     GWVHITGLTGERGRKIEIRYLHDYVPSGDNSYILKGSRNESYRARFNWFVFREAEILN
                     WPGDLTAEQISAEAVYTKVETTGSFRCSNQLFEDINRIWWRSQTDNMHGGIASDCPNR
                     ERSPYTGDGQVACVTVMHNFDARAFYNKWIHDIIDAQDTVSGYVPNGAPWQPGCGGGV
                     AWGAAISIMPWEFYVHYGDTAILKESYSGMKGYVNYMLKFTNSEGIMHSRSAGAGSRP
                     NPWMNLGEWSPPYEFPPEEMVHTFYLWRCADITARVASVLGNENESLAFHSIAGKTRQ
                     AFMTHFWDEIAGSYGKYGGNIFALRMGVPEDKKSRVLKALASDIMANDGHLDTGIFGT
                     QFFFEVLAENGLNELACEAMNKRTVPSYGYWIEKGATTSWEEWGRGVSGNHPMFGGGL
                     TWLYRNLAGFKSDEANPGYRHIIFRPQPAGDINEVSYYNETPLGRGGITWKRKGLKFS
                     MDITVPAGATATVYIPATGPESVTGNGLPLDKLEEITMVKQEAGYTVCKTGSGNYSFE
                     SDIR"
     gene            complement(15504..16415)
                     /locus_tag="PK288_02890"
     CDS             complement(15504..16415)
                     /locus_tag="PK288_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPM17895.1"
                     /translation="MKLGFVSAIVPELSFEELIDFASAMEFQCVEVCCWPLGKAERRY
                     AGVTHIDVDTLDDQRCAVINNHLKVKGVEISALGYYPNPLDPDPARRNFYVSHIRKVI
                     DASALLGINRINTFVGRDKNKSVEENFEIFQEVWKPVIQYAETKGVKVGIENCPMLFT
                     ADEWPGGNNLATTPAIWRRMFNMIPSASFGLNYDPSHLLWQQMDYVRPLYEFREKIFH
                     VHFKDAKVYTDKLDEVGIMATPLEFHSPKLPGLGDIEWGRFISALNDIRYKGFACIEV
                     EDKSFENSLEERKHAVVLSKRYLSSYM"
     gene            16985..19147
                     /locus_tag="PK288_02895"
     CDS             16985..19147
                     /locus_tag="PK288_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17896.1"
                     /translation="MKSKIAVTGICLLLSLNLAAQREIQSLNGIWEVEESIDAGKVPA
                     RFGHKVRVPGLTNTATPSFEGVDRFYGREYYTNRWVQPTLMKLDIDADTMKIGYSLQK
                     RNYFWYRKTFETKPGFEAVILKINKAQFGSMVLVNGKKAGEYDGCFTSRQYDITPYLK
                     KKGTNVVIVRIGAHPAVLPPSVPAGNDFEKRHWTPGIYDDVSVIFTNYPYIETVQVAP
                     DIHKGEILVSARVITRPGTETIKLTGKIREWKSGTEVSGLTKDFPTGGDSRATVTFSI
                     PLRNARLWAPWDPFLYTLNLSTGSDDMDVRFGMREFRFDTPTKRAYLNDKLIFLRGSN
                     ITLHRFFDDSLCVNHPWEEEWVRKLLTGLPGKYNWNCFRFCIGPVPDKWFDIADEAGL
                     LIQNEYFIWSYRPYWDRNLLKTHLQDWILDCCNHPSVAWWDINNETREPILTELISEI
                     RSLDLSGRAWDNGYNLPEGDNDPVEDHNYKFYSSRPGTNGWHQQKFMNGMAEKTTNSP
                     HPTAHACVLNEYGWLWLKRNGQKTLLTAPIYDSIAKGYTSQQRQELYAYLLAAETEYF
                     RAHRNYAGVLHFDYLTGDFHGAITGDIFRDPEKLEPVEVYDEYFSEIFKPLGVYVNYF
                     PENVIAGTETEIPVMLVNDEYHRISGNIEIRILDESGEEVANSSGPFSIAELSQYTGK
                     IKIRMPEKEGAYRIHTYAVQPDGSKTLCRRKINITSEK"
     gene            19422..20282
                     /locus_tag="PK288_02900"
     CDS             19422..20282
                     /locus_tag="PK288_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM17897.1"
                     /translation="MKTKSAIFISIWLLIFSNTYSQDGKEILLLIRGDDIGSSHTANI
                     ACIDSYRDGIMRSVELMVPGPWFPEAVRLLNENPGLDVGVHLVVTSEWNDIKWRPLTC
                     CPSLVDKDGYFYPMIWQREGFPQGTALKDANWKIEEIEAEFRAQIEMARRYVPRISHI
                     NCHMVCESCAPEIADLIDRLAREYGLNIDPHERGYESISLWGRNDTTAETRIASAVKT
                     LQNLKPGKYFFIDHPGYDTPEMRAIWHIGYENVAADRDGVTKVFTSSKVKDVISERNI
                     KLVSYRDLTK"
     gene            complement(20355..21077)
                     /gene="deoC"
                     /locus_tag="PK288_02905"
     CDS             complement(20355..21077)
                     /gene="deoC"
                     /locus_tag="PK288_02905"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPM17898.1"
                     /translation="MIEVSELAKMIDHSILHPSYTDSDLEEQCRIALRYNVASVCVKP
                     YAVSRASKLLKGTGVKVGAVAGFPHGNSSTEVKVFEATRACLDGAEEIDMVINIGKVL
                     GGDWDYVEQEIRSVTSACHQNGAIIKVIFETDLVTRDEDKIRLCELCTLAGADFVKTS
                     TGYGFVRQPDGNYNYKGATIADVELMRKHSGPNVQVKCAGGVRTLDDLLKMRAAGASR
                     SGATATAAILEEARKRFSGSAG"
     gene            21535..22464
                     /locus_tag="PK288_02910"
     CDS             21535..22464
                     /locus_tag="PK288_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HPM17899.1"
                     /translation="MKRSNFLKSALFVPAMVSGHNGFFQAAGESSGIRILGADRSFTL
                     WKPEMDPAYHVGLNEVVLVETSHGMPGLVTRDGVFREPGPESVINPQTGPIFVDGIEP
                     GDMLAIDILDISVGDWGYCSGQIFELKDGYVIFSKSLKLPMMPMLGSIGISPSEGTLD
                     TKAPGETGGNMDCREVRSGSTVVFKARVKGALAGMGDAHALQGDGEVTGQGVETDAKA
                     IVRFRKLTGFSCDRPVIIRNDSISTVGAHRDLEEAAWQATDDMVKLVQQMTGRNDKES
                     YLLVSLTGMLKINQIVDPAKGARMEVPAWVFGI"
     gene            22478..24592
                     /locus_tag="PK288_02915"
     CDS             22478..24592
                     /locus_tag="PK288_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17900.1"
                     /translation="MKILIILFYILASCVIPLESGLPDADLAYCDFTKVKPGKFGGFA
                     ARGFPEYHYVPRHFTDGWSIVNNRGPEEWYIIESDGKHSLQYLGYNSTVWTNEFTYPL
                     LCYGDILWQDYNLEVTLTPFDRSDLNGIVFRYRDGRHYYLFALDREDKITLRYRDGEK
                     GFREDGWHEIASSVFRTDPEKTYKFRVEVAGNQIRCFIDGREIFNVNDNRYPGGKVGL
                     FATCPVAFHEVRVTASDAARQKYLRERSEEEARVEALRADNPRPKLWKKIDTREFGVA
                     RALRMGDLNGDGRTDFLLIQNMPFFGENYNQITCLTAIDLDGTILWQYGKPDPENAWL
                     SYDVAVQIHDIDGDGKTEVIMAQGQWIKILDGRTGTEKSRYPVPSSAILPGEKSWLEY
                     KHYYRRDLLPYLNVDCFAFGDLRGTGKPHDVIIKDRHTRMWAYTNKFELLWTASANLG
                     HFPFFHDGNNDGRDEVYIGYSKFGPDGTLIWSLDSLMGEHSDGICVGDFSLAGGPDRI
                     FIGGSDDGVLFLDAGGKILKHHRVGHAQTPSIGQYRPDIPGLEFCNINFWGEPGLITL
                     YNCSGEEIISFELIHAGSPVQPVNWRGDGTEFIFLSPNIEEGGLVDGWGRRVVMFPDD
                     GHPDLAWTVRDLTGDPRDELIVWDTESIWIYTQDRPFKGDRIYKPVRPAEYNESNYAT
                     MYSWPGWEKFRREQPRGNTESR"
     gene            24638..26449
                     /locus_tag="PK288_02920"
     CDS             24638..26449
                     /locus_tag="PK288_02920"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HPM17901.1"
                     /translation="MIRRNFIKTAGGAIALSPLEAFSQRKREPKNPKVSAKAEYYPSR
                     IEVKDDKIYADTMTLSAIFENGFLISLRNKKSGEEFIREFSREIHSPLQLVYTRDEIM
                     EFSDKNFTNLRINQVSDKRVEIIFSCWHGDGVVSIFTDDETGDLIVEPSAYSSRPGVL
                     ACRWSVPGIKPEMELVAPLFQGIKIKLNDELIHNSRWAWPSYWEAGLAILHSGTGGFY
                     VHSRDHKYRYKALKTGSENDPYVLSFDSEAYGPVDNNLAAGNLAWRINVYDGDWHVPA
                     ARYRDWLWEAFSLKKEEEKRKAWINDVRFAVSWCPGETEILDALSKKISPSKVLLHYP
                     EWRTDIYDQNYPEYKASEKGRNFTKRCKEYGFRVMPHFNANDMDPSNPVYNLIRDFQY
                     RDIETKKILGWSWIDGRSIGVPESNDSRVHNRKNNVMVKVHPGLSMWRSVLGEQIKKA
                     CDDLGLDFVFIDVTLVMHNLYNSLVESTTSAEGMKLLIDHIGRLGNGLVVGGEGLNEI
                     SMQGLSFAQAHLFKSWHSSVEGLERTGGCDLNQFLFGRLCKIIGYSFLSGKNKDEETR
                     MQVHLEHGGIPTVTITSADEINNPNPAVKRMLDIANS"
     gene            26845..29019
                     /locus_tag="PK288_02925"
     CDS             26845..29019
                     /locus_tag="PK288_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPM17902.1"
                     /translation="MKTYGLICLLFSMVLTGSISVSAQENYRYDGKNVVLSNGLITRT
                     IHTRGDSIILKGLTLKGSDLNYVGHISPEFGFLLNDIPVTSLSGWKVIGVDPAKDAGK
                     GSGAALRLKGTGPAEGLDLKITYLLYGDLPLIRKYIEFVNRSGSDMKLEAVDSELMKL
                     GFDYVSSWVYHNYARMKHLGPFTGNWDDPVVVIHNTVLRAGIAIGNEAPGVLKRTVYH
                     TTDRNAEAGLTHPGQDFPFRKWLRPGESWQSPPTFIALYNGTDDGYEVLNTVVGEYVR
                     NHMGTRISAIRNKPLFIYNTWNPFRTFVNDSLIREVAKAASECGIQEFIIDDGWQYNA
                     GAETTKKGWGSNYGDWLVDRNKFPGDLSATFDYIRSLGMKPGLWMSIGSATEDASVFQ
                     SHPEWFVIDRNGRTGNLHSGQEAGGFYTSCFGTDWFDYIKSVIVNMVRKHGLAYAKLD
                     FSVATSAYVNDPGISGCYATDHPYHRDHAESFIVIYQNVLRLFDELHAEAPDLFIDCT
                     FETAGKLQLQDYAFAKHAEGNWLSNIENPFPTGALRMRQMAWWRSPALPAGSLVIGNL
                     PLDSKNFEVDIKSLIGTLPIILGDPRKLSPEKRASIKKWAEWMTRMQDRYDYMSFRQD
                     LPGFGEPREGSWDGWSRINTDNRKGGIIGVFRHGSNEKSRTVCIERLDPEARYTVLLA
                     PEGKEIAVLTGSQLSSEGFRVDLPEKYDGDIYEISLITDSRQ"
     gene            29140..29307
                     /locus_tag="PK288_02930"
     CDS             29140..29307
                     /locus_tag="PK288_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17903.1"
                     /translation="MGRAAAIHVHIPNIAARCGESMLIRDETTGKFTNSEMANEYITP
                     EYRKPWALPVI"
     gene            29632..31032
                     /locus_tag="PK288_02935"
     CDS             29632..31032
                     /locus_tag="PK288_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HPM17904.1"
                     /translation="MKKRLFVMLIAVLASYPALSQANPWKGASVDFSHGRLKVSENKR
                     FLVHEDGTPFFYLGDTAWELFHRLTREEAEKYLENRRAKGFTVIQAVVLAELDGLNTP
                     NTDGNRPLLNNDPQKPNEKYFEHVDWVIKKAKEKGLYIGLLPTWGDKVDKKWGVGPVI
                     FNRDNAFGYGQWIGNRYKDYPNIIWINGGDREGGGSNYEVWDALGRGIKSVDANHLMT
                     FHPLGESSSSVWFHNSPWLDFNMCQTSHAQRSYSIYRKLIEHDYNLRPVKPCMDGEPR
                     YEDHPVNWKPEELGWFDESDVRQALYWDLFSGSFGHTYGCNPVWQMKTPEHEPVGFAR
                     NNWYDVLDLPGASELIHARRLMESRPFLSRVPDQSLIVPADYSDTDYIAATRGDGYAF
                     VYFPTGKSAVINLQMLGAEGIHSFWYDPRTGESTLIETIDEIRPFRFKPPSGGKGNDW
                     ILILDDVSKGFFLPGQ"
     gene            complement(31185..32678)
                     /locus_tag="PK288_02940"
     CDS             complement(31185..32678)
                     /locus_tag="PK288_02940"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPM17905.1"
                     /translation="MEHYMGIDIGTSGCKAVVFDESGNQVSKSYREYDIISPSPGMAE
                     LNTDEVISACFEVIREAAGRVIPSTLKGIGISSQGEAFTLIGSDGKALCNALVSSDSR
                     ASEMIDPWVEEFGEERLYRITGHTPHPMFSLFKLLWIKKNMPGIWEKTRRIMCFEDLL
                     QYRLGIANPSMGWPLAGRTMLFDVIRHEWNNEILEKSGIKREMMSLPLQSGSLAGIVD
                     SSIARNLNLPENVFVVTGGHDQPCSGLGAGAIMPGVAVYSSGTVECITPAFAKPVMSE
                     ILRKGNLCTYDHAAPGLYATVAFSLTGGNILKWFRDEFGSEEKYLAEKTGRDAYELLL
                     EIMPDEPSRLLVLPYFTPSGTPYFDVTARGTIMGLDLTVKREEIIRALLEGVAFEIRL
                     NLEILRQSGCPVDELRIMGGGARSLKHVQLKADVTGMPITVLDVPEAGCMGVAMHARS
                     YHTKGHIGQIVQEWVKPLARIEPSETDHYNRKFLQYRELYPKLKTLY"
     gene            complement(32687..>33453)
                     /locus_tag="PK288_02945"
     CDS             complement(32687..>33453)
                     /locus_tag="PK288_02945"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPM17906.1"
                     /translation="IGLKLFLSDIEVLTAPGSPFEKLRVMIHLDHVQWDADKPLLEWD
                     MNRFSMIMYDASSLPLDDNIEFTARFVENFGSKIVIEGACDEIVDATGDVRSELTTPA
                     QAEKYLGQTGVDLIVANLGTEHRASAADLKYHGDLARKISSITGPKLVLHGTSSVSKE
                     QITGLFDDGIVKVNIWTTLERDSSPVLFEDMVRNASGVAGIEKTEHMVEKGLLGRNAG
                     RHSQASLSHYTTTYRQEIIFNEMKRIVAGYFDLWYK"
BASE COUNT         9132 a         7835 c         7766 g         8720 t
ORIGIN      
        1 cccttttcgt cgtcagaccc gccgatgctc actccattgg taagtgccct taatccaatc
       61 tcaaaatcaa ttatcctcaa taactcctct ttcctgtgag ttgtgatgac ggtatgctcc
      121 ctgacaaacg gggagcggtc gtgccataag ggtgatttcc acaacacatc ggcttccagt
      181 acagcagtag attcatctgt ccaggttgtc agccggatta cctcctgcga aatattctcc
      241 attatccatc cgtcggcaat actctgatca tttatataaa tctggtgcca cgcccagtag
      301 atgcccctgt gataaggatg gtcctccgga gcttcttccg tgagggtatc cccgtcgaga
      361 gaatagagcg ggtgcaggta attgttgcag atgtactggt tgccgataac cccgggttcc
      421 ctctgataat ggaacaccgg atcccctgat tcgtaaagat caagcccctg ttcattctgc
      481 ctgaactcaa agccttcctt tctttctgcc gaagtgccgc atgaaatcag aaaacagaaa
      541 aataccatca atatgccgga agtgtctttc aaaaggaaat aatctgacta aaataataaa
      601 tacctgataa ctgatgtgaa tccttcatga tcatgtgtca ctttcatgat gttcttcact
      661 gctgtaacat gaatattcat actgtgctga agattaagga tcagcatatc acgacaagtt
      721 acaaaaagaa agtcagtata aaaacaattc tgattttttt gtgatatccg gaaagagtgg
      781 caggaacttt tatgttatgc ccggctgtga gccggatagc gccatgggaa tgatcggggc
      841 aggagccgca ggataaagga agatattaaa ggcattttcc tgtattccaa acattttcac
      901 tttaaatgaa aaaagcaggg ctgtgataat ggacagcaag atcatattga catatattag
      961 tgtaataatt aatatatatc aactacttga ttgatatata ttaattaatc caatatctgt
     1021 ttttatcttt caggccagtc aattcattag gtttgtcaaa cacaggctgc agcagtcatg
     1081 gttgccggaa ccggatcacg atactcagta cgtaagaatt acatccgtat tcctgttcag
     1141 gtcagtcgtg attgagctgc ttcctgatac aatggtgtag ggtgtgaaaa aaatcctgtc
     1201 ctttccgttc gcctgatatt ccggaagttc agcactgaat tttccctgat tgtttgtttt
     1261 gccctgcacg gcaattttcc cggtattgtc aaatatagtc acatcccggt ttttcccagg
     1321 tctgcccggt ttatccatca gaacagcatt cagggtccag tacacggtaa atgaatgatc
     1381 ctggtcggtg gcatcaatac cgaactcctg ccctctgaca aaatttgacc tgaattttat
     1441 aagctttgcc acacaatcat cacagcctga gcttcccatg cggaatgttt taaagggatc
     1501 attgccggtt gcgatcaccg tgttgcaata aatatctgaa ttggcggcgc ctccgtacat
     1561 tgaagctatc caggctgcag gcacattggt tcttatgaca ttgccgtaaa actgtcctcc
     1621 atagtatctg ggcccgccgc agatatacag ggccgctgta attaccctgg aggaaggatc
     1681 cgtcttttca acggcgaatt cattgtcata cacgtaattc tccccgccgc ttgcactgta
     1741 atatattccc cagcacatgg gaatatattc tgccggccca aaatcccttg ccgttatgaa
     1801 gatccggtta tcatgtatcc tgttgccggc agatgcattc cgggaacctg gtgcggcatt
     1861 gtaatcacca agcctgattg cagcggtact gtattcttcc cttccgtatt cgcatgtcgg
     1921 tttcgatgtc tcaattttga aaagattgtt gaaaacttca gtatatctcg atacataaat
     1981 acccgaaccc tgttttggct cgaacctgtt attgaagatc ttagagctgt cgcccgtacc
     2041 catgatgctg taatgatttg taacccactg gtcgtttaca aaaagattat catgcacaac
     2101 agagtatttt cctgttaccg acaggcatcc ctggcctccg aagaactccg aattggacac
     2161 ttccgatggg tttgtgcccc ggatcagaac cgaacaatgg ttaacatgcc gctgaatgag
     2221 aaagggaaca tccgcctcaa acctgcagtt ttttatcttt gtctgcaata tgtcggcgct
     2281 tccgcagctg atacccgcag tcacaatttt gacgttttcg aggacaacca ttacgtcggg
     2341 tgcggatgac tggatacccc acgactggat tcccggggta cctgcttcaa ttatcccgtt
     2401 tttaatcgtg ataacccctg aaccgctgat atgcggcagg gatggcaagg gcaggttccg
     2461 ctcaaagtcg ccggtaaaat catagaaggc aggtgtgacc ggaggatatg attccgcgat
     2521 aatatgatcg taatgagcca gtggagtcgt atttccgata atgcttatac ccgcatccat
     2581 tgccggacga atatcaatct cgtcaaccag gcagtcggtg tctgccttaa tcctgaccct
     2641 gtattttcct gcaggcaatc catgcagatg agcaaataca aatccgcctc ccagacgggg
     2701 agatttcttc tcaaccgggc agctgatcct cgtgccgtcg ccgtattttg tggagcatac
     2761 cacgttccgg ccctgcgcat cctcaacaaa aatgctcact ttcatttcat catccgggta
     2821 tttcttcata tcatgataat acctgccagt aatcccgcac attgcaaaat acgatctgcc
     2881 ggcaaccggc agattcacat attgcgacac gatttcatca ccgggctgca ggctcataag
     2941 cttactgccc atgaaaacat gaacctcttc agtgttacgg atctcagccc cgggtgcctt
     3001 tgaaagatcc cagccggacg atccttcttc aaaccccgaa ttcggaatat gttcatagcc
     3061 gccggcagca tatctgagag tatatccgtt caggtcgagg gtaacattat ttccaaggaa
     3121 caatgcgctt ccttctgcac tgatatcatt cacaagtacg aatctcctgc cgtctccggc
     3181 atagctcccc ggagaactga cgggaatctc accgggcgtg agataccggt gtgcaacggg
     3241 ggaatactgc ggaagaagct gcgggcataa caataacccg acaagcatta aatgcacctt
     3301 tttcatatga tattaaattt atatactact aaatattaaa tgattcacat ggaacccgca
     3361 tgaattatat tttcatgctt gtttttgatt ctgtatcatg tggggttcat atctgtctgc
     3421 tttatattat ccgctgaccc gataatgacc ttgttctgca tatcccaggc tgaccccgct
     3481 gaaaccatcc tgacatgatt aagtccttct tgccttcatc atttattttc cgtattcaaa
     3541 caaagaactg cacggggaca gacatacctg ggccaatctg ccggttcaaa cttaaaatca
     3601 ttcaacactt tgtaggagca tattaaatga aagcttttat aagatgtata ttatcaaaca
     3661 aaaataatca tttcgcatta ataaggtttt gaaataatca ttaaatttgt catcctgtca
     3721 actattttcc ttgcaaatta aatgagaagc tttttccaga actttcaaat gactctcctg
     3781 acggcagcgg tactgtatgc tgcatcatca caggtgttgt atgcccagga agacatgatg
     3841 catttcagca aggttttcaa cagggcaaaa ccatacagga tcttcctgcc tgcagattac
     3901 cacaagcttg acaggcatta cccggttatc tattattttc atggcaacac cggtgatcac
     3961 aaactcgaca ttccgggaat aagcgggctt gtcaatgcca acggtgttat actggttgcc
     4021 tggaacggca ggtctgagga gtcagatctg aggccataca atataggcaa ccactcaaac
     4081 atcaaataca atatacagtt cagggattat tttcctgaac tggttgatta tatcgacaaa
     4141 acctaccgta ccattcctga cagatcagcc aggggtgtta tcgggcacag tatgggcgga
     4201 ataatgtcat tcttccttgc gggtcagtat cctgatctca tctgcgctgc tttcagctca
     4261 aaagggtcac ccgaattttt catcggaacc ccggcgcgcc acactctgta tcaggtcagg
     4321 aacatgttta aaaacctcat cggcataaag acaggatttg cgacaagcac ggaatgcgaa
     4381 ctgtattatc ttaacaatga agttattaat ggtgcactga acgagaaaga tcttgatttc
     4441 agacttttca ggtatgaagg gagccatgat atcactccca aacagttcag cgatgcattt
     4501 gagtttgtgg ctgaggcttt caggaatcct gaaccggcac ctgagcgctg gcatcactca
     4561 gatatatacc cggatttcag gatatggggt tatgaggtgg aaagtaatct aaacgaacct
     4621 ggtttcatcg acatgaaagg agttaccagg ggcggattcg gaatttccac aaaatcatgg
     4681 gagcctgacg gcaagccgct aaagggagtg aacataaggg tgaaaacagc tccgttgtat
     4741 tcacccaata ccgtatatac aattctcgac tacagtcatt caaccggttc tgcaaagatt
     4801 tgtcaactta ctagcgacaa atcgggaagg atcaccgttg atacagacaa tgagaatcac
     4861 cagatcggaa ttttcaggaa aaaggatcct ccagaaattg tatttatcaa acataatgta
     4921 aatgacaaag gatcatttct cgatcagaaa gatgaaaatt cactgaatct gacttttctc
     4981 aacaggggag ggagcaaggc agagaaggtc ttagttacac tgagcaccga tgttccggga
     5041 gtttccatcg ccaatccggt tattgaactg ggctctcttc cttcatgtga gctgaaagcc
     5101 ttgccgcagg gatttaaaat tactgcccgc aacaaaccgc cctccgacgg ttctcctttc
     5161 atgataagat tcaaccttgc gatcagggac ggaaaaaaca gggtgtggaa ggatgaattt
     5221 tatgttcctg tcttttttga tgttcctgaa tttacgcatc ttgggatcga cgatggcgac
     5281 agcgaggttt tcggcagcgg caacgggaac aacatcgctg aaccgggaga aaccgttatg
     5341 atttatgaga tatcaaacgg aagcaaaaga ctgaggctat attatgatga cccatacata
     5401 gacggggaac ggttatatga tgagatacag cccgacaaat ggggtgatgg atattccctg
     5461 tcatcactta ttcatatttc tgacgtctgt cctccggggc ataaaataag gtttcttgcc
     5521 tgctatgaag taaaggactg gcaaaagatc aggagagatg taacatgggg taccttcaca
     5581 ctaaccgttg gcggtgaatt gcacggaaaa caataatcaa acccaataat ccaggaaaca
     5641 tgcaaaaact gaaaaacaaa cggccggaaa ggatcctgat cctttttgca ctggcaatta
     5701 tatcaatgtc cgttcatgtg acaaaagctt cagttccggc tgatccggca gccaaacggg
     5761 ctgatgacag ggggaaggtt tttatagtga atgctccgat acgtgatatt gcagagtttc
     5821 gcaaactggt tatccaggct gcacgcctga agccgtacgg ggaagttcag gtgaacatca
     5881 gcgcacttgc cggcggcgga tttcacgagg ttcctgaggg gaacaatttc tggcaccagt
     5941 atgccagtta caatgcaact cttttcaaat tctttcccga tcccgcaatt gcacccttca
     6001 taccggctga ttttgtcaaa agaaaccgtg aatacctgct tgccagggcc agaatactga
     6061 gggaatatgg cattaatgcc gagttctggt gcaatgaacc aaactttctg ccagatgctt
     6121 tttttgacag atatcccggt ctcagggggc cgagggttga tcatccgcgg cggggtaacc
     6181 atcctgcatt ttctctttgc atggaccttg aacaaagcag gaaaatggtt gccggtatga
     6241 ttgaggaact gctaaaaaac gttcctgaga tcagaacctt tgttttcaag accaatgatg
     6301 cggggtcagg gatatgctgg tccgactggc agtattccgg tccgaacggg ccctcacact
     6361 gcaggggaat atccatgggc aggagagttg cttcgctgat ggagctgttc aacgagggag
     6421 caaaaaagac cggaaatgaa ttgacgatat atcttgccga atctatgttt tccgatgagg
     6481 aaatgactga tatactgaaa tatcttcctg caaactgtca tgcactcgga acggattccg
     6541 acaactcaat atccatcgga agcaatattt caggttatta tcccgtcaga gggcttcttg
     6601 atccggttga tctgttaagg aaaatcgatc agatgaaaga tgacaggtac aaaaccgttt
     6661 ttatcaattt caaatcgtat tatgacaggg gatacgaact gatgccggtc attgaaaagg
     6721 tgattgatct gatcactgac aatatgggac aaccagctgt tgaacgggaa attgcaagga
     6781 tgctgcaggt tgaggatctg tgcaggaaat gggccggtga cagggaatct aaaaagcttt
     6841 ttgatgcaat tatgctcctg gaagatgcaa aaaaatataa gaactcagcg gtcggcaatg
     6901 taacaggcat ttactggggt gtttccacga ggcatatcaa cagaccgctg gttttcgctc
     6961 cccagctgct gaccagggaa gaggaagcct acttcctccc ctatgtattc aatccatcca
     7021 ctgaagaggc aaggatggat tacacggata ttcacggaag ccacaacagt gttccttccg
     7081 gggttattcc acgctatgtt tcgatgattg ttagagccgc cgggttaatg gagggcattg
     7141 gaaaggacat ccctgaatcc atgttcatac aaacatgggc cagggcattg agacttcatg
     7201 caggcattat gcggagcgcc ggaaattttg ccgaggccca ggccatcaga gaccgcaatg
     7261 ctgataagct cagccggcct ccccacaggc ccggcaagga gccgacctgg gacggagatc
     7321 ccgacctaca ggcgtttaac accgtaatgc gcgatgaact ggataatgcc ggggaactca
     7381 ttgaactcct cagcggcgga ggtacggaca tcctctgtca tgcttccgac cctgcacagg
     7441 aggatacttt cctgctcgga cccggactga tacgccagct gaaactaaag caaaaaatca
     7501 tgctggctca ctggacggat attgaaggtt atctggccac cccgttcaaa taagttaatg
     7561 aaaataaata tatttgcagc atcaaacaac cacgttgaaa tgaaaacacg attgatcctt
     7621 gcagctgttt taattatact gatctcttct tcatgcggac aaagaaagag aggcgacaaa
     7681 actgcggtga agggagatcc gtttccgaaa gagatggtgg aattcgtgcc gtatgaaaac
     7741 aatcctgtgt tcacagggac agggcaggac acctgggaca ggacaatcag ggaaagagga
     7801 ttcatactta atgagaacgg cacctacaag ttgtggtaca ccggttacaa cgggcctgat
     7861 acggtgacaa aataccttgg atatgcaacg tcacctgacg gaataaactg gaccagatat
     7921 gaggggaatc ctgtttacaa caagcgatgg accgaagata tgtttgtttt cagggaagac
     7981 aataattatg tcatgtatgc cgaaggcaga aatgatgttg cacatatcct gatttctgaa
     8041 gatggcatca actggcagga ccagggtgat attaaaattc tcaccacaaa gggcgataca
     8101 atacccggac cgtacggtac tcccactgta tggcatgaaa ataacctctg gtatttattc
     8161 tacgaaagga atgatgatgc catctggctg gcagtatcca acaatcagaa aacatgggtc
     8221 aatttccagg atgagccggt gcttgaaaga ggaccggaaa actacgattc gggtgctgtg
     8281 gcagtaaacc agatcctgaa attcagggga aggtattata tgttctatca tgccacctcc
     8341 gatccttcat ggatagaacc cgggaattct gccgtatgga cttccaatgt ggcaatgtca
     8401 gaagatctgt atcactggac aaaatatccc ggaaatccga tagttgaggg agatcattca
     8461 agcccagtgc ttgtgcctga cggcgataaa ttcaggcttt atacaatgca tcccgggatc
     8521 tggctgtatt tctcaaaatg atactgccat gcatcagggc tgaagaatgc ttatccaacc
     8581 tttgccagcc tgacagtcat attcagattc agccagctgg tttccaggca aagtcagttc
     8641 ttaattcttc ttcagtctga ttctgcagta ccacttgtcg cattgcataa aaataatgtc
     8701 aggttctatc tcgaaaatag tggtatagca tgatcccgca ttccggatga tcttatccgt
     8761 tttccatgtt tgtcctccgt cattgctgat tgacaggctt gtatagcctt tccccggata
     8821 ttcaaataat cctttgtctc ctgcatctga tattatcgac catccttcag gaaatctttt
     8881 cccccagctt aaagcaatgc ggccgtcgtg aagtgtgatg agacaggggt cgcatccccg
     8941 gtcaaatcct gtatacaggg gaggcgtcca ggtttttccc gtatcggcac tccacgatga
     9001 ataaagagga aaatggtgtc ccgagcgcat gacgcaaaga agtcgtccgt cgtttaattt
     9061 tgttattgcg ggttcaacaa atccttcccc gaccggttct ccggcgtgag gaacagcaac
     9121 agttgaaaga taattccagg tgcgtccctt gtcccccgac ttcacaataa ttgtccgcat
     9181 catgtattca agttctttca ttgcatccct gttcgagggc agaacagtat cagtttccat
     9241 gttcccatac attgtcataa gccacagatc aggagatatt tcaagaatgg ttctgtaaac
     9301 gaatatgccg taccaattgt caatgccatt atccggcatg cagcctccag ggatattgaa
     9361 aaccggttcc tccgttgtta ttgtcttcag gttgtcagtg gaacgccagg ctttgccctt
     9421 gtatacaccg ggttcaataa aggaagtagg gccgtcaaga gcataaaaca ggccgtcttt
     9481 tacaaaaaca gctctttccg gagctccgtt cacatctctg aatacagtat ccgtgagttc
     9541 tgataaggat ctgcctgcat cgtggcttat aaaatttttg aaatagacag atccgtcaaa
     9601 gtttctgaag cagcgtgtcc atttatcatc cgagttcatt gaggcaatca cgggtttgcc
     9661 tgtgataaca taattaacgg ttgttttttc attgatacga ttacagcttc ccaaagccag
     9721 acataacagg agcagagccg ggaaaccagt caggcgactg accggcaggc tttttggttg
     9781 tgatatcatg gtaatgtttt ttatacggaa acggggatca gatttagtca ggacagatct
     9841 ttttttattg cttttacggc tgaccccggt cccggatgca ttattttctt atttgtccgt
     9901 tcccttctat gatccactta tatgtagtaa gctcattgag agccatggga ccccttgcat
     9961 ggagtttctg ggtcgcaatg cctatttcgg ctcccaggcc gaattcagct ccgtcggtaa
    10021 aagcggtaga tgcattcgaa tagaccgaag aagcgtcaac aagcttaaag aaacggttta
    10081 tcctttcgct gttttcggaa attattgctt cgctgtgctt tgaaccaaac tcatcaatgt
    10141 gggcaacagc ctcttcgaat gatcctgctg ttttaactgc cattttatag gaaaggaact
    10201 cggttccgaa tgaatctttc tcagcttttt tgagaagttt cgccggatac ctgccttcga
    10261 gtgcacggta agctttttca tcagcatata taaccacatt gttgccggca cataattcca
    10321 caatataacc caggtcgtca agccgctttt cgtgaattat caggcaatca agggcattgc
    10381 atacacttac cctcctggtt tttgcattgt gaacaactgc cctgcctgtt tccctgtttc
    10441 caaactcatc aaaataggta tggcacacac cggctccggt ttcaattacc gggatggatg
    10501 aattgttcct tacatagtca ataagatcac gacctccgcg gggaatgatc agatcaacat
    10561 aatccctcgc attcagaagc tgaattgttt cttcccttcc ggccggaagg aggaaggcag
    10621 cgttctcgtc aatcccgttt tctgcaagga ccttccggat aattgagata atggcggtat
    10681 tggaatttat tgcgtcgctt cctcccttaa ggatacaggc attgcctgat ttaaagcaga
    10741 gtgagaacac gtcaaaggtt acgttgggtc gtgcctcata aataacgccc acaactccga
    10801 aaggtaccga tacccttttt acaatgagcc cgttggggcg gacttcctcc gacaggatcc
    10861 ggcccagcgg atcgggcaga ctggccacgt tcctcatatc accggctatg ttttttatcc
    10921 tttcgggaga aaggagcaac cgatcatact ttggatccga cttttccatc aggtcgagat
    10981 ccttcctgtt ggcttccagg atcatatccg tactttcaat cgctctttcc gctactttca
    11041 ggataacgtc cctgattgca gactgatcca gggaagacag ggttttgccg gctttcagca
    11101 ctttcctgaa cacctcttcc atggaacctt ttgtgatgtc tttattcttt tccataacac
    11161 tcatccccat cattgattaa ttttcagctt taaaccgtta cattgccttt tatctcctgg
    11221 ctgataacaa ggaaatcaca atgaataaaa ggtttgctat gtttctgtcc gataagtttt
    11281 gtagttttct cagaatcaaa tttcgtctgc cccaaaccaa tatacttacc ttcctcatcc
    11341 agtacccgca caatatcacc gctttggaaa taaccttcaa ccccggtaat tcctataaac
    11401 agcagacttg ccttttcact cagtatggcg gcttttgcgc cattgtttat aaatacagcg
    11461 cctttggcaa aagtatcgga atgattgagc cattttttca cgccgctctt tttatttaca
    11521 ctggcatgaa aatgggtaaa aggtacatcc ctgcccctga ttatatccgt tattatcgaa
    11581 tccctgttgc catttgcaat atagacatca atacccaggt tggagatctt cctcgcagtt
    11641 gaatacttcg atagcatccc gcccctgcca aaacctgatt tgatggctga tatgccgccc
    11701 gatacattat gagaatcagg atatacttcc cttataagtt cagctcccgg actgttgggt
    11761 tccgaattgt aaataccgtc aacattggta aggagaaaca gagtatggca gtccatcatc
    11821 gcagaaatca gacccgacag ctcatcattg tcggtgaaca taagttcgtc aatggaaatt
    11881 gaatcatttt cattcagtat gggcagaaca ttgctgtcga gcattgcggt aatgcaattc
    11941 ttcatattca gataatgacg tctgtccatg aaactttcct ttgtagccag aagctggcct
    12001 acctgcattc cgtatttccc gaacaggaac tggtaatttg ccataagctt aacctgtccg
    12061 atggaagccc atatctgttt tgcacctaca atatttgtcc ttttcgacgg tacaacctcg
    12121 ctcctgccgg cagcaaccgc tcccgacgtt acgaggatca cttttttacc ctgcttgctc
    12181 aatacggaaa tatcctctac aatccgaagg attctgctta cattaagcga tccgtccttc
    12241 tgggtaataa cattgctccc gaccttaacc gttatccttt caaattttga taattcagtt
    12301 tccaatttat aagctatgct actgtttttc ttaaattttc ccgcaaaaat aactgttttt
    12361 atgattaaat gacagtaatc acaaataaat taactgttta tgaacggccg caaagtactg
    12421 tttcggggaa tttccagggt atcctgccaa agcttcaaca attcaggcat acttcagtat
    12481 tatcaggata tacaaatgtt cagtaaacag gttcatccac tttggaaaac ttaattcata
    12541 tgagccgggc ctgtcttcag ctcctatctt atatcacttt caaaactgta attcccggat
    12601 ccggttttac agacggtata gcctgcttcc tgtttaacca tggttatttc ttcaagctta
    12661 tccaacggaa gtccgttccc ggtaactgat tcagggccgg ttgcgggaat atacacggtt
    12721 gcagtggcgc cggcaggaac agtaatatcc atgctgaatt tcagtccttt tcttttccag
    12781 gtaattccgc ctcttccaag cggggtttcg ttatagtatg aaacctcatt tatgtcgcct
    12841 gcaggctggg gcctgaaaat gatatgacga taccccggat tcgcttcatc tgacttaaag
    12901 ccggccaggt tcctgtaaag ccatgtcagc ccgcctccaa acatcggatg gtttccggaa
    12961 acgcccctcc cccactcttc ccatgatgtt gttgcacctt tttcgatcca gtatccgtaa
    13021 gacggtacgg ttcgtttgtt cattgcctca caggcgagtt cattaagtcc gttttcagcc
    13081 aggacctcaa aaaagaactg ggtgccgaag atacctgtat caagatgccc gtcatttgcc
    13141 atgatatcgg aagccaacgc tttcagcacc ctggattttt tgtcttcagg aactcccatc
    13201 cgcagggcaa agatatttcc gccatattta ccgtaacttc ccgcaatctc atcccagaag
    13261 tgcgtcataa atgcctgcct tgtttttccg gctatcgaat ggaatgccag ggattcgttc
    13321 tcattgccaa gtactgatgc cacccgggca gtaatatccg cacatcgcca cagataaaaa
    13381 gtatgtacca tctcttctgg aggaaactca tagggtggtg accattcccc gaggttcatc
    13441 cagggattgg gcctgctccc ggcgccggca gacctggaat gcatgatacc ttcgctgttt
    13501 gtgaacttaa gcatgtaatt aacatagcct ttcattccgc tgtagctttc cttaagaatg
    13561 gcagtatcgc cgtagtggac atagaattcc cagggcatga tactgatcgc ggcgccccag
    13621 gccacacctc ctccgcaccc gggctgccag ggagccccgt tgggtacata gcccgacact
    13681 gtgtcctgtg catcgattat gtcatgaatc catttgttat agaatgccct tgcgtcaaaa
    13741 ttatgcatca cggtaacaca ggctacctgt ccgtcgccgg tatatggtga acgttccctg
    13801 ttgggacaat cgctggcaat tcccccgtgc atattgtcgg tctggctcct ccaccatatt
    13861 cggttgatat cctcaaagag ctggttggag catctgaaac tgcccgttgt ttcaaccttt
    13921 gtataaaccg cctcggcact gatctgttct gccgtcagat cgcccggcca gttaagtatt
    13981 tccgcttccc tgaatacaaa ccagttgaac cgggccctgt aggattcatt tctgctcccc
    14041 ttcaggatgt atgaattatc acccgaaggc acataatcgt gcagatacct gatctctatc
    14101 tttctgcccc tttcacctgt aagcccggta atatgaaccc atcctgatat ctcctgtccg
    14161 aaatcaacct tgaagcggcc ttccgaaagc ttttcgagtt taaccggttt caggatctcc
    14221 attaccctgt cggggtatgc catatgggcc cttaaggcgc cctccggtgc ttttctaaga
    14281 acggcattct cccagcctga gtcgttgaaa ccaggctcgc accatccatc ctgctccaga
    14341 cgggcgtcat aatgttcccc gtaaaacacc atgtccataa ggatagggct tctggcaacc
    14401 ttccatgttt catcagtcaa aatcacatct tcagaaccgt cggtatagct tatgtgcatc
    14461 tgaagaataa ggcggggtgt accatatcct cccgcccagt atttggccgg attatagaat
    14521 ccgttgccaa ccagtgcccc aagggcattt tttccctgcc tgaggctgcc ggtaacatca
    14581 taggcgaggt aaaaaacttt ataccccttg aaattatcct ccaggggtat attttcctta
    14641 ttcaggtaag gacgttttcc ataattggtc tgattcggga ccagaacatc atcacccact
    14701 tttttcccgt tcagatacag ttcgaaatat cccaggccag taacatacgc aacagcgttc
    14761 gcaacttgtt tgctgacttc aaactctttc cggaattccg gtgccggcgg aggcagtaag
    14821 tcgggaagct ttgctcccgg gccggatggt ttaggaagag gttcctctcc ctgccatgga
    14881 gcgccaatcc attttgcctt ccattcacac tggtcaagaa tacccattga ccaggaagca
    14941 gggttgctcc acgcggaaac atttccatcc ctgtcccaga cccttacctg ccagtaacag
    15001 gtctgacggg atttcaatgg tttacccttg taacggacgt tgcatgactg gtccgaaacg
    15061 accttgccgc tgttccagag gtcagccctt cctgaccgca aaaggccggg ggagcctgcc
    15121 accctgatct cccaggcagt ctggagctgt ccgcgggcat cagggccctg ttcatttacc
    15181 catgagagcc tgggctccgg tacatccgtc acaggcggat ccctgaggta ttcacatgtc
    15241 aggttcgcgg gggtaattcc gcagtcgggc cctgcacaat actgcaacag gcaaaggaga
    15301 aaaaggaaaa taaacctcat tatgataagt ttgtgatata caatggattc tatgatttgt
    15361 cagaacagtt cacatatgac tgcccgaaat gagaatatca gaaaaccagg atagcatacc
    15421 ggcatgataa tgtcaataat atgggtctgg accgggagga tcagcggaat tacaatccgc
    15481 attattacaa ctgttccgcc ggcctacata taggatgata aatatctttt actgagcaca
    15541 acggcatgtt ttctttcttc tagagaattt tcaaagcttt tgtcttccac ctcaatgcag
    15601 gcaaaaccct tgtatcttat atcatttagt gcggaaatga accgtcccca ttctatatcg
    15661 cccagtccgg gaagcttagg tgaatgaaac tcaagcggag ttgccataat gccgacttca
    15721 tcgagcttgt ccgtgtaaac ctttgcatcc ttgaaatgga cgtggaagat cttttccctg
    15781 aattcataaa ggggtctgac ataatccatc tgctgccata gcaggtgtga cggatcgtaa
    15841 ttcaggccaa aactggctga aggtatcata ttgaacatcc ggcgccagat ggcaggagtt
    15901 gtggcaagat tattgcctcc cggccactca tctgccgtaa acagcatcgg gcagttttca
    15961 atccccacct ttacgccttt ggtttcggca tattgaataa caggcttcca cacttcctga
    16021 aaaatctcaa aattctcttc aactgatttg ttcttatccc ttccgacaaa ggtatttatc
    16081 cggttaatgc ccagtaatgc cgatgcatca atcaccttcc tgatatggga aacatagaaa
    16141 tttctcctgg caggatccgg atcaaggggg ttcggataat atcccagagc cgatatctca
    16201 actcctttta ccttcagatg gttattaata acggcacatc gctggtcgtc gagggtatca
    16261 acatcgatgt gagtaacgcc tgcataccgc ctttcggctt tgcccagcgg ccagcagcag
    16321 acttcaacac attgaaattc cattgcagat gcaaaatcaa tcagttcctc aaagcttaat
    16381 tcaggaacaa tggcgctgac aaatcctagt ttcatattct taatttttcc tgtttgtgtt
    16441 ctcctgcttt attgttggac ctctgtacgt ctccaaatta gtcccttgta aaaacaaaga
    16501 tataccttaa cttcatttaa ataaaacttt cataaagact aatgcaataa gggatggaat
    16561 atcttcctga cccggggtat gcaggttcaa tgaaaactga gcccggggac ggctcgcagc
    16621 ggtaaaagaa ttgattatca gctcccggat actggcggcg gttgttgtac ggaaaacggg
    16681 agtatcccgg aaagtgctgc tgacgaaggg aatatcctgc cggtaaccct caattatctt
    16741 taactgagtc tgtccgctct acggctcagc cggaaaaagt tccctgtatt tccgttaatt
    16801 atgacgcaat cagtatattt attgtatatt tcatccttag gtagtttatt tattaaaggt
    16861 cggcatcccg cgtgatttaa cccgattaaa ttcattagta tttatgtttc aggtcagtca
    16921 tgcggatttc atctcatgcg tagtttctta atgcagcctg aagttaatgt taaaccataa
    16981 acaaatgaaa tcaaagattg cagttaccgg aatatgcctc ctcctgtccc tgaacctggc
    17041 ggcacaaagg gaaatacaga gcctgaatgg tatctgggaa gttgaagaaa gcattgatgc
    17101 aggtaaagta cctgcaagat tcggacacaa agtcagggtt cccgggctta ccaatactgc
    17161 cactccttca tttgaaggag ttgacaggtt ctatggcagg gagtattata ctaaccgatg
    17221 ggtgcagcca accctcatga agctggatat cgatgccgac accatgaaga tcggatacag
    17281 tcttcagaaa aggaattatt tctggtacag gaaaactttt gaaacgaagc ccggattcga
    17341 agctgtgatc ctgaaaatca acaaggctca gttcggatcg atggtgctgg ttaacggcaa
    17401 aaaggcaggt gaatatgatg gttgttttac atcccgccag tatgacatta ccccttatct
    17461 caaaaagaaa gggaccaatg ttgtaatagt aagaatcggg gcgcatccgg ctgttttgcc
    17521 gccctccgta cctgccggca acgattttga aaaaagacac tggaccccgg gtatttatga
    17581 cgatgtctcg gtcattttta ccaattatcc atatattgaa actgttcagg ttgcccccga
    17641 catacataaa ggagagatac tggtcagtgc aagggtgata acccgcccgg gcacagaaac
    17701 cataaaactg acaggaaaga taagggaatg gaaatccgga accgaggttt cgggattaac
    17761 caaggacttt ccaaccgggg gagattcaag ggcaactgtt acattcagca ttcctctccg
    17821 taatgcaaga ttatgggctc catgggatcc gttcctgtac actctgaatc tttcgacggg
    17881 aagcgacgat atggatgtac gtttcggaat gcgtgaattc aggttcgaca ccccgaccaa
    17941 aagagcctac ctgaacgata aactgatttt tctcagggga tcgaatatta ccctgcacag
    18001 gtttttcgat gattcacttt gcgtaaatca tccctgggaa gaggagtggg taagaaagct
    18061 gctcaccggt ctgccaggaa aatacaactg gaactgtttc aggttttgta tcggtccggt
    18121 ccccgacaaa tggtttgaca tagctgacga ggccggcctg ctgatacaga atgagtattt
    18181 tatctggagt taccggccct actgggacag gaatctgttg aaaacccatc tgcaggactg
    18241 gatacttgac tgctgcaatc atccctctgt agcctggtgg gacataaata acgaaacccg
    18301 tgaacctata ctgactgagt tgatctcgga aatcaggtca cttgatttgt caggacgtgc
    18361 ctgggataat ggatacaatc ttcccgaagg tgataacgat ccggttgaag atcataatta
    18421 caagttttat tcttcacggc cggggactaa tggctggcac cagcagaaat tcatgaacgg
    18481 catggcagaa aaaactacaa actctccaca tcccactgct cacgcttgtg tgctgaatga
    18541 atacggatgg ctgtggctta aaaggaacgg ccagaaaact ctgcttaccg ctccgatcta
    18601 tgattccatt gccaaaggat atacaagcca gcagcgccag gagctgtacg cttatcttct
    18661 ggcagccgaa accgaatact tcagggcaca ccgaaattac gcaggggtcc ttcatttcga
    18721 ttatcttaca ggtgatttcc atggtgcaat cacaggcgat atattcagag atcccgaaaa
    18781 actggaaccc gttgaagttt atgatgaata tttttccgaa atattcaagc ctcttggggt
    18841 atacgtcaat tacttcccgg aaaatgtaat tgcaggcacc gaaaccgaaa taccagtcat
    18901 gcttgtaaac gatgagtatc atcgtatctc cggcaatatt gaaatcagga tcctggatga
    18961 atcaggtgaa gaagttgcaa attcatctgg tcccttcagc attgcggaac tgtcacagta
    19021 cacaggcaaa ataaagatca ggatgccgga gaaggaaggt gcttaccgga ttcataccta
    19081 tgcagttcag cctgacgggt caaagacgct ctgcaggaga aaaataaata ttacatcgga
    19141 aaaataaact cacaggcgcg gccatcatgt ttttcttgtt taacggagga tcatcgtgcc
    19201 aggaaaagaa ggacagaatt aaatacaccg gatggcccaa ccggttaagg agtgaggacc
    19261 ggggcgcttt gcccccggga atgcctcagg ggaatacgct gaaataacgg tttgaggatg
    19321 aaaaaccaca ggctgatccg gtttctgaag aaggagtaag ctgtattaat acttttattt
    19381 aataacttca catcggatca aaaataaaac cgaaaggaaa aatgaaaaca aaatctgcaa
    19441 tatttatttc aatctggctg ctgatatttt caaatactta ttcccaggat ggcaaagaaa
    19501 tattactgct aatccgcggc gatgatatag gctcctccca cacggcaaac attgcctgca
    19561 tcgactcgta cagggatgga ataatgcggt cggttgaact gatggtcccc ggtccatggt
    19621 ttcctgaagc agttcgtcta ctgaacgaaa atccggggct tgacgttggc gtacatctcg
    19681 ttgtgacaag tgagtggaac gacattaaat ggcggccact gacgtgttgc cctagcctgg
    19741 tagacaaaga cggttatttc tacccgatga tatggcagag ggaaggcttt ccccagggca
    19801 ctgcactgaa ggatgcgaac tggaagatcg aagagatcga ggctgaattc agagcccaga
    19861 ttgaaatggc cagaaggtat gtcccccgca taagtcatat caactgccac atggtatgcg
    19921 aaagctgtgc accggagatt gcagatctga tcgaccggct ggccagggaa tacgggctga
    19981 acattgatcc tcatgaaagg ggctacgaaa gcatttccct gtggggcagg aatgacacaa
    20041 ctgctgaaac aagaatagcc tctgcagtaa aaacccttca aaacctcaaa cccggcaaat
    20101 atttctttat tgatcatccc ggatacgaca cccccgaaat gcgtgcaata tggcacatag
    20161 gatatgagaa tgtggctgcc gaccgcgatg gggtcactaa ggttttcaca agcagcaaag
    20221 tgaaagatgt tatatcggaa agaaatatta agctggtatc atacagggat ctgacaaaat
    20281 aatattttaa tgattggaac ccctggttcg gtccattcct gatttattac atcccggttc
    20341 aaccgggagc taaatcatcc ggcagatccc gaaaatcttt ttcttgcttc ttccagtatg
    20401 gctgcggtag ctgttgcacc cgaacgggaa gctcctgcag cccgcatctt cagcagatca
    20461 tcaagagtgc gtactcctcc ggcgcattta acctgaacat ttggtcccga gtgcttacgc
    20521 atgagttcca catcggctat ggttgcccct ttgtagttat aattcccgtc cggctgcctg
    20581 acaaaaccat aaccggttga tgtctttaca aaatcagctc cagccagggt gcaaagttcg
    20641 cacagcctga tcttatcctc atcacgggtt accaggtccg tttcgaagat cacttttatt
    20701 attgccccat tctggtgaca ggcacttgtt acgcttctga tctcctgctc cacataatcc
    20761 cagtctccac caaggacctt gcctatattg attaccatgt caatttcctc agcgccatca
    20821 aggcaggccc tcgttgcttc aaaaaccttc acttctgtcg aactgttacc atggggaaac
    20881 cctgcaaccg cccctacctt taccccggtt cctttcaaca gtttcgaagc ccggctcact
    20941 gcatacggtt ttacgcaaac cgaggccaca ttgtagcgta aggctatcct gcattgttct
    21001 tcaagatcac tgtcagtata cgaaggatga agaatggaat ggtcgatcat ttttgccagt
    21061 tctgaaactt ctatcatgta ataaattttt atgaattaaa taattaaata ctgcatccga
    21121 ccccctgtcc aaacctggca caataaacca tttccaggaa acaaagactc tgcagcctgg
    21181 gaaatcttac accctgtaat ccagttaagt ccattcgtca gcacaagtcc atgggaaatt
    21241 cgtgatcatt atttaaatgc cgggttttgc cagatatttt tattcttcta tattgcaata
    21301 tataaagcat taggtttatg aaacccaaat gtatgacact ctcaaacact tgcaaaaata
    21361 attttcagca tgaggtttcc agcatatcat gttaatcaga attaaaatat tataaagccc
    21421 gtacggttga tcaaaaacat aaacactaat gaataaaaac gcgttcaatc atatgtggtt
    21481 ttcaggtctg cggggcatac aggcatccca cctttaaaaa taaattattt gctaatgaaa
    21541 aggagtaatt tcttaaagtc agctttgttt gtgccggcaa tggtttcggg ccacaatggt
    21601 ttttttcagg ctgctggtga aagttcaggt atcaggatac tcggggcaga caggagtttc
    21661 acgctatgga aacctgagat ggatccggca taccatgtag gtctgaatga ggtggttctc
    21721 gtagaaacga gtcacgggat gccggggctt gttacacgcg acggcgtctt cagggaaccc
    21781 ggtccggaaa gtgtcataaa tccgcaaacg ggaccgatct ttgttgacgg tatagagccc
    21841 ggcgatatgc ttgcaataga catccttgac ataagtgtcg gtgattgggg ttattgcagc
    21901 gggcagatat tcgaactgaa ggacggatat gttatctttt ccaaatccct gaaactcccg
    21961 atgatgccga tgctgggatc gattggcatt tcgccgtctg aaggcacact cgatacaaag
    22021 gcaccgggcg aaaccggcgg gaacatggat tgcagggaag tacgttcggg cagcactgtg
    22081 gtattcaagg cgagggtaaa aggggctctg gcaggcatgg gagatgcaca tgccctccag
    22141 ggagatggtg aagtaacggg acagggtgtt gaaactgatg ctaaagctat tgtcagattc
    22201 cgtaaattaa cgggattctc ctgcgatcgt cctgttataa tcaggaatga ttcgatttca
    22261 acagtaggtg ctcacaggga tcttgaagag gcagcgtggc aggcaactga tgatatggtt
    22321 aagctggttc agcagatgac aggcaggaac gacaaagagt catacctgct tgtgagtctt
    22381 accggaatgc tgaagataaa ccagatagtg gatcctgcga agggcgcaag aatggaggtc
    22441 cctgcatggg tattcggaat ataaaataac gctaaaaatg aaaatactga taattctgtt
    22501 ctatatcctg gcatcatgtg tcattccact tgaatccggg ctaccagatg cagacctggc
    22561 atattgtgac tttaccaaag taaaaccggg gaaattcggc gggtttgccg ccagggggtt
    22621 tcccgaatat cattatgtac cccggcattt caccgacgga tggtcaatag tcaacaacag
    22681 ggggccggag gagtggtaca taatcgagtc ggacggtaag cattcactgc agtatctggg
    22741 ctataattcc accgtctgga ccaatgaatt tacctatcct ctactatgtt acggcgacat
    22801 cctctggcag gactataacc ttgaagtaac cctgacgcct ttcgacagaa gtgatctgaa
    22861 cggtattgtt ttccgctacc gtgacggtcg tcattattac ctgtttgccc tagacaggga
    22921 ggacaaaata acactcaggt accgcgacgg agagaaaggg ttcagggagg acggatggca
    22981 cgaaattgcc agttcagttt tcaggacaga tcctgaaaaa acatacaagt tccgggttga
    23041 ggttgcgggc aatcaaatcc ggtgctttat cgacggacgt gaaatattca atgtcaacga
    23101 caaccggtat ccaggcggaa aggtgggact ctttgcaacc tgtcctgttg catttcatga
    23161 ggtaagggta acggcctcgg atgcagccag gcagaaatat ctcagggaaa gatccgaaga
    23221 agaagccagg gtagaggcgc tgcgtgctga taatcccagg cctaagctgt ggaaaaagat
    23281 cgataccagg gaattcggag ttgcccgggc cctgcggatg ggtgacctga acggcgatgg
    23341 ccgtacagat tttctgctta tacagaacat gccatttttc ggagaaaact ataaccagat
    23401 cacctgcctt acagccattg accttgacgg aacgatactt tggcagtacg ggaagcctga
    23461 ccctgaaaat gcctggcttt cctatgacgt ggcagttcag attcatgata tcgacggaga
    23521 tggcaaaacc gaggttataa tggcccaggg acaatggata aaaatactcg atggcagaac
    23581 gggtacggaa aaatcacgct atcccgtacc ttcttctgca attctgccgg gagaaaagtc
    23641 ctggcttgaa tataaacatt attacagaag ggaccttctt ccgtatctta atgtcgactg
    23701 tttcgctttc ggagatttaa ggggtacggg taaaccgcat gatgttataa tcaaggaccg
    23761 gcatacaagg atgtgggcat acacgaacaa gttcgagctg ctttggaccg catcggccaa
    23821 tctgggacat tttccgtttt tccatgatgg caacaacgac ggcagggatg aggtgtatat
    23881 tggctattca aaattcgggc ctgacggtac cctgatctgg tcgcttgatt cgctgatggg
    23941 tgaacattcg gacggtatct gcgtaggaga tttttcactg gcaggcggac ccgacaggat
    24001 tttcatcggg ggcagcgacg acggagtatt gttccttgac gccggaggga aaatattgaa
    24061 gcatcacagg gtcggtcacg cccagactcc cagtattggt cagtatcggc cggatattcc
    24121 cggccttgaa ttctgcaata taaatttctg gggcgaaccg ggtctgataa ccctctataa
    24181 ttgctcaggc gaggagataa tcagtttcga gctcattcat gcaggctccc ctgttcagcc
    24241 agtgaactgg aggggtgacg gtaccgagtt catctttctt tcgcccaata ttgaagaagg
    24301 cggacttgtc gacggctggg gaagaagagt ggtgatgttc cccgatgatg gtcatccaga
    24361 tcttgcctgg acagtcaggg atcttaccgg cgatccgcgc gacgaactga tagtatggga
    24421 tacggagtca atatggattt acacccagga caggccattc aaaggtgaca ggatatataa
    24481 acctgtccgg cctgcggaat acaatgaatc aaactatgca acaatgtatt catggcccgg
    24541 atgggaaaaa ttcagacgtg aacagccacg ggggaacacg gaaagcagat gaaacagccc
    24601 gatacaatct caatggttca tcaaaaacaa ccggaatatg ataagacgaa actttattaa
    24661 aacggctggc ggggccatag ccttgagccc tcttgaagct ttcagccaaa ggaaaaggga
    24721 accgaaaaat cccaaagtgt ccgcgaaagc tgaatattat ccttccagaa ttgaagtaaa
    24781 ggatgataaa atctatgctg ataccatgac cttgtcagcc attttcgaaa acggattcct
    24841 catttcactg agaaacaaga aaagcgggga ggaatttatc cgggaattca gccgggaaat
    24901 tcattctcct ctgcagctcg tttatacccg tgatgagatt atggagttct cggataagaa
    24961 tttcacgaat ctaaggatca atcaggtttc tgacaaacgg gtggaaataa tctttagctg
    25021 ctggcatgga gacggtgttg tttccatctt caccgacgat gagaccggtg accttatcgt
    25081 tgaaccgtcg gcttattcct caaggcccgg agtactggcc tgcaggtggt ccgttcccgg
    25141 tattaagcct gaaatggaac ttgttgcacc attgttccag ggtataaaga ttaaacttaa
    25201 tgatgaactc atccataact cacgctgggc ctggccatcg tactgggaag ccggcctggc
    25261 tatccttcat tcaggaacgg gaggatttta tgtccattca cgggatcaca aatacagata
    25321 caaagccctt aaaaccggat ctgaaaatga tccgtatgtt ctcagttttg attctgaagc
    25381 ttacggccct gttgacaaca acctggctgc cggaaacctg gcatggcgga taaatgtata
    25441 tgatggcgac tggcacgttc cggcggcccg ctaccgcgac tggctgtggg aggccttttc
    25501 cctgaagaag gaagaggaaa aacggaaagc ctggatcaat gatgtcagat ttgccgtaag
    25561 ctggtgcccc ggtgaaactg aaatcctcga tgccctttca aaaaaaatct ccccgtcaaa
    25621 ggtactttta cactaccccg agtggcgcac tgacatctac gaccagaatt atcctgaata
    25681 caaagcaagt gaaaagggca ggaattttac taagagatgc aaggaatacg gattccgggt
    25741 aatgccccat ttcaatgcca acgatatgga cccgtcaaat cctgtttata acctgatcag
    25801 ggatttccag tacagggata tcgaaacaaa gaagatactt ggctggagct ggatcgacgg
    25861 gcggtcaatc ggcgtgcctg aatcaaacga cagcagggta cacaacagga aaaacaatgt
    25921 gatggtcaag gtccatccgg gattaagcat gtggagatcg gtcctcggag agcagatcaa
    25981 aaaagcctgt gatgatctgg gactggattt tgtattcatt gacgtcaccc ttgtcatgca
    26041 caatctttat aacagcctgg tggaatcaac aacatctgca gagggaatga aactcctcat
    26101 cgaccatatt ggaagacttg gcaatggcct cgtggtcgga ggagaggggc tgaatgaaat
    26161 ttccatgcag ggcctctcat ttgcccaggc acatcttttt aaaagttggc atagctcggt
    26221 tgaaggcctt gaacggacag gtggctgtga cctgaaccag ttccttttcg gaaggctttg
    26281 caaaataata ggctacagct tcctaagcgg gaaaaacaag gacgaggaga cgcgaatgca
    26341 ggtgcatctc gaacacggtg gaatacctac cgtgacaatc acgtcggctg atgaaataaa
    26401 taacccaaat cccgcagtga aaagaatgct ggacattgca aacagctgat acaaggacgt
    26461 cagcattcgt taaaattacg gctgacaaag caggacacat gacccatggt gcaatactcg
    26521 ggatgcaaaa tgtcaggaat cactgcagac aataccagga agatcccctc ccgggagggg
    26581 ccaggggtgg atttatttaa agaacccgga cgcaggctat gcacatcgca gctccactgg
    26641 aatctgtagc tacagtgaag gaggtgtgaa gtagtatgca catttcttcc gaagcttaag
    26701 cggagaatga ggttacagaa taaataaaag atgatctctt ttatatgagt taaccgaatt
    26761 atctgattga cttatatcat tatattcatt gatatatatc attatctgga agaatatttt
    26821 tcatctgtca aatgatcaaa gatcatgaaa acttacggtc ttatttgttt gctttttagc
    26881 atggtcctta ccggatcaat ttcagtgtca gcccaggaaa attacaggta tgacggcaaa
    26941 aatgttgttt tatctaacgg gctgataaca agaactatac acacaagagg cgattcaata
    27001 atcttaaagg gactgaccct gaagggatcg gacctgaact atgtcggaca catttcgcct
    27061 gagtttgggt ttctactgaa tgacataccc gttaccagcc tgtcggggtg gaaagttatt
    27121 ggtgttgatc ctgcaaagga tgccggcaaa ggcagcggcg cggctctgag attgaaaggc
    27181 acggggcctg ctgaaggact tgatctgaaa ataacatatc tgctgtacgg cgatcttccg
    27241 cttatccgca agtacatcga gtttgtcaac agatctggat cggacatgaa gcttgaagcc
    27301 gttgacagcg aattgatgaa acttggtttt gattatgtat catcatgggt atatcacaat
    27361 tatgcccgca tgaagcacct tgggcctttc accgggaact gggatgaccc cgtggttgtg
    27421 attcacaata ccgtgttgcg tgcaggcatt gccattggga atgaggcccc tggcgtttta
    27481 aaaaggactg tttatcatac gactgacaga aatgcagagg caggactgac acaccccggg
    27541 caggattttc cgttcaggaa atggctcagg cccggcgaat catggcaatc gcctccgacc
    27601 ttcatagcgc tttacaatgg gacagatgat ggatatgaag tgttgaacac ggttgtgggc
    27661 gaatatgtca ggaaccatat ggggacccgt atctcagcaa tcaggaataa gccgctgttt
    27721 atttacaaca catggaatcc cttcagaaca ttcgtaaatg actcactgat tcgggaggta
    27781 gctaaggctg catctgagtg cggcatccag gaattcataa ttgacgacgg ttggcaatat
    27841 aatgcaggag cagaaacaac aaaaaaagga tggggcagta attatggcga ctggcttgtt
    27901 gacaggaaca agttccccgg cgacctttcc gccacctttg attatatcag gtcgcttggc
    27961 atgaaaccag gtctgtggat gagcatcggt tccgcaacag aggatgccag tgtatttcag
    28021 tcacatccgg aatggtttgt aattgacagg aatggcagaa ccggcaatct gcactccggc
    28081 caggaagccg gcggctttta caccagctgt tttggtaccg actggttcga ttacatcaag
    28141 tcggtcattg ttaacatggt caggaagcat ggcctggctt atgcaaagct tgatttttca
    28201 gtcgctacca gcgcatatgt aaatgatccg ggtatttcag ggtgttatgc tacggaccat
    28261 ccatatcaca gggaccatgc tgaatcattt attgtgatct accagaatgt tctcaggctt
    28321 tttgatgaac ttcatgccga agctccggat ctatttattg attgcacctt tgaaacagca
    28381 ggcaagcttc agctccagga ttatgcattt gcaaaacatg ccgaagggaa ctggctttct
    28441 aacattgaaa atccgtttcc caccggagcc ctgagaatga ggcaaatggc atggtggaga
    28501 tcgccggctc tgcctgccgg atcacttgtc ataggcaacc tgccgcttga cagtaaaaat
    28561 tttgaggtgg atatcaaatc tctcatcggt actttaccta ttatactggg ggatccgagg
    28621 aagctttccc cggaaaaaag ggcctcaata aaaaaatggg ccgaatggat gacaaggatg
    28681 caggacaggt atgattacat gtcattccgg caggatctcc cgggctttgg cgaacctcgt
    28741 gaaggctcat gggacggctg gtcaagaatc aacaccgaca accggaaggg tggcataata
    28801 ggtgttttca ggcacggatc aaacgaaaaa tcaagaaccg tttgtattga aaggcttgat
    28861 ccggaagcac gctataccgt cctgctcgct cctgaaggaa aggagattgc tgttttaacc
    28921 ggcagccagc tttcatccga aggtttcagg gttgacctgc ctgaaaaata cgatggcgat
    28981 atttatgaga tcagtctaat taccgacagt agacaatgac ggaagtgtcc agatatcgaa
    29041 atgttttgtg aacggccgga aatcgccggc aggtctgtct gtttcagaaa atcaggattt
    29101 ggaggatttt gaagctactg cccctaatct cagaccggaa tgggcagagc cgccgcaatc
    29161 catgtgcata tacccaatat tgcagccagg tgcggagaat caatgctgat ccgggatgaa
    29221 acaacgggaa agtttacaaa cagtgaaatg gccaatgaat atattacacc cgagtatcgt
    29281 aaaccatggg ccttacccgt aatttgatgt gtaaccacga gtcgcacgga gttagtgacg
    29341 gaatgaagac catgaataac taaaactccg taagaccccc gttaaaccct gtgaaatccg
    29401 ggcttaaatc ataagaacct ctaaataaac cttatagcag aatcaaatta aaataaatca
    29461 tggtaacggg aagaaaattt atacctgaat cagcaatggc tacagttgct gcgctggtat
    29521 caacggatgt tttttcctcc agccatatta actacaatga caactgattg gttttccgga
    29581 taaatattaa taaattacag tctaagattg ttcaatataa acacgattga aatgaaaaaa
    29641 agattatttg tcatgctgat cgctgttctg gcttcatatc cagcattatc ccaggcgaat
    29701 ccctggaagg gagcatcagt cgatttttcc catggcagac tgaaagtatc agaaaacaaa
    29761 cggtttctcg ttcatgagga cggaactcca ttcttttacc tgggagacac tgcatgggag
    29821 ttgtttcaca ggcttaccag ggaagaggct gaaaaatatc ttgagaaccg ccgtgcaaaa
    29881 ggtttcacgg taatacaggc tgtggtactg gctgaactcg acggattgaa taccccgaat
    29941 actgacggga acaggccttt gctcaataat gatcctcaga agcccaatga gaagtatttt
    30001 gaacatgtcg actgggtaat aaaaaaggca aaggaaaagg gcctgtacat tggattgctt
    30061 cccacctggg gcgacaaggt ggacaagaaa tggggtgtgg gccccgtgat cttcaacagg
    30121 gataatgcat tcggttacgg gcaatggata ggcaaccgtt acaaggatta tccaaatatt
    30181 atctggataa acggaggaga cagggaaggc ggcggcagca attatgaagt atgggacgcg
    30241 cttggcagag ggatcaaatc ggttgatgca aatcacctaa tgacatttca tccattggga
    30301 gaaagcagtt cttcagtatg gttccacaac agcccatggc tcgatttcaa catgtgccag
    30361 acgagtcatg cccaaagatc atattcaatt tacaggaaac tcattgaaca cgattacaat
    30421 ctccggccgg taaagccatg catggatggc gaaccaagat atgaggatca tccggtaaac
    30481 tggaaaccgg aagaactggg atggttcgac gaatcggacg taaggcaggc gctgtactgg
    30541 gatctgttca gcggatcatt cggtcatacc tatggctgca atcctgtatg gcagatgaaa
    30601 actcccgaac atgaaccggt tggcttcgcc aggaataact ggtatgacgt gcttgacctg
    30661 ccgggtgcat ctgaacttat tcatgccagg cgactgatgg aatcgcgtcc ttttctttca
    30721 agggtccccg atcagtcgct gatcgttccc gccgactatt cggataccga ctatatcgca
    30781 gcaacaaggg gagacggtta tgcctttgta tatttcccca caggaaagag cgcagtcata
    30841 aatcttcaaa tgctcggagc cgagggaata cattcattct ggtacgatcc aaggacgggg
    30901 gaatcgaccc tgatagaaac aatcgatgag atcaggcctt tcaggtttaa gcctccttca
    30961 ggcggaaagg gtaatgactg gatactgatc cttgatgacg tgtcaaaggg attttttctt
    31021 ccggggcaat gaaacaggca ttgactgtcg tcatgacata agcaggtgcg gcaattattc
    31081 cgccgtgaat aaaaggccat tgattatatc ccgttatatg cacgtcagcc aatcagcctt
    31141 aagctgaaac agacggttca gcaaccaccg gagagtcacg gtgatcagta taaggtttta
    31201 agcttcggat acagttccct gtattgcagg aatttccggt tataatgatc tgtttccgac
    31261 ggttcaatcc tggccagcgg tttcacccat tcctgtacaa tctgtccgat atgacccttt
    31321 gtatgatatg acctggcatg cattgcgaca cccatgcacc cggcttcggg aacatcaagc
    31381 accgtaatgg gcatgcccgt tacatcagct ttcagctgaa catgcttgag cgatctggcc
    31441 ccccctccca taattctaag ttcatcgacc gggcaacccg attgtctgag gatctcaagg
    31501 ttgagcctga tttcaaaggc aacaccctcc aggagggccc ggattatttc ttcccgcttc
    31561 acggtcaggt caaggcccat aattgttccc ctggccgtga catcaaaata tggtgttccc
    31621 gacggggtga aatagggcag taccagaagg cgcgaaggct catcaggcat aatttcaaga
    31681 agcagttcat aggcatcgcg gcccgttttt tcagcaaggt acttttcttc actgccgaac
    31741 tcatccctga accatttcag aatattgccc ccggtcaggc tgaatgcaac agtggcgtat
    31801 aatcctgggg ctgcatgatc ataggtgcag agatttcctt ttctgaggat ttcagacatt
    31861 acgggttttg cgaaagcggg ggtaatgcat tccacggtcc cggatgaata gacggcgaca
    31921 ccgggcatga ttgcacctgc acccaggcct gagcagggct ggtcatgtcc cccggtgacg
    31981 acaaatacat tttctggaag gttaagatta cgggctatgc tgctgtccac aatgccggca
    32041 agggatcctg actgcagggg taatgacatc atttcccgtt taatcccgct tttttccaga
    32101 atctcattgt tccattcatg cctgatcacg tcaaacagca tcgttcttcc tgccagaggc
    32161 catcccatcg aaggattggc aataccgagc ctgtactgga gcagatcctc gaaacacatt
    32221 atccggcggg ttttttccca gatcccgggc atattctttt ttatccagag cagtttaaac
    32281 agggaaaaca tcggatgagg ggtatgaccg gttattctgt aaagccgctc ctctccgaac
    32341 tcttcaaccc aggggtcaat catctcactg gccctcgaat cggaggaaac aagcgcattg
    32401 caaagagctt ttccatcact tccgatcagt gtaaaagcct cgccctgaga tgaaatccct
    32461 atgcctttga gagtggacgg gatcacccgt cccgcagctt cccggataac ttcaaaacat
    32521 gctgaaatga cttcatccgt attcaactcg gccatgcccg gagaaggtga tataatatca
    32581 tactccctgt aggatttcga gacctggttc ccggactcat caaaaacaac agccttgcaa
    32641 ccgctggtcc cgatatctat tcccatgtaa tgttccatat gccggttcat ttgtaccaga
    32701 gatcaaaata acccgcaact atccgcttca tttcattaaa aatgatttcc tgccggtaag
    32761 ttgtggtgta atgagaaagg gaagcttgtg aatgtctgcc ggcatttctc ccgaggaggc
    32821 ctttttcaac catgtgctct gttttttcga tcccggccac tccagatgca tttctgacca
    32881 tatcttcaaa aaggacgggg ctgctgtcgc gttcaagggt agtccagata ttcactttga
    32941 caattccatc gtcaaaaaga cctgttatct gttccttact gacagacgat gtaccatgca
    33001 gtaccagttt gggtccggta atactgctta ttttccttgc aaggtcacca tgatatttca
    33061 ggtcagctgc acttgccctg tgctctgtcc cgagattggc cacaataaga tcaacccctg
    33121 tctgccccag gtatttctca gcctgagcgg gggttgtgag ttcactcctg acatcaccgg
    33181 ttgcatcaac tatctcgtca caggcacctt caatcactat cttactgccg aagttttcca
    33241 caaacctggc agtaaattca atattgtcat caaggggcaa tgaggatgca tcatacatta
    33301 tcatcgaaaa cctgttcata tcccattcca ggaggggttt atcggcatcc cactgcacat
    33361 gatcgagatg gatcattacc ctgagctttt caaaaggcga accgggggca gtaagcacct
    33421 cgatatcgga aaggaacagc ttaaggccga tat
//
LOCUS       DAOOYG010000017        33343 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1108_length_33343_cov_7.815850, whole genome shotgun sequence.
ACCESSION   DAOOYG010000017 DAOOYG010000000
VERSION     DAOOYG010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33343
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1108_length_33343_cov_7.815850"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..433
                     /locus_tag="PK288_02950"
                     /pseudo
     CDS             176..433
                     /locus_tag="PK288_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539871.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            584..1507
                     /locus_tag="PK288_02955"
     CDS             584..1507
                     /locus_tag="PK288_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPM17907.1"
                     /translation="MKTDTKQIRVSGTITFCLILFLSVFSLKGQNNDEMPLKVGMVSD
                     IQYADIDNSGTRHYRASVGKFSEAVSKFREEDVDMIVSLGDFIDNDFKSYKPLIKITR
                     GINIPVYFVLGNHDFSVRENFKRKIPGMLNVRSRYYSLTRDNWRFIFLDGTDISTFSR
                     RKKDPEYNRARSILDSLKSVGAPNAHSWNGGIGKAQIEWLEQEIDNSHKAGQNIFIFC
                     HFPVYPEDQSENLWNADKIRKIVESGNGKIVFMTGHTHRSNYCFNNGVHYVSLRGMVE
                     TDENSFAIAEIYDDSIMIKGYGAEKDTVIKW"
     gene            1667..1831
                     /locus_tag="PK288_02960"
     CDS             1667..1831
                     /locus_tag="PK288_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17908.1"
                     /translation="MTVHKLQYLSNATGNRNLLQHDGILIEALQYYREGFHPDHFTGL
                     PEWNHQAIQP"
     gene            2523..3833
                     /locus_tag="PK288_02965"
     CDS             2523..3833
                     /locus_tag="PK288_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM17909.1"
                     /translation="MKPVTRRNFIRTGAAAALGISAVPAAAFAADVDAQPVKVGVVGM
                     GNRGTSHVNCLLTIGGVEIVALCDLFENRAVNAAEICVKAGRKRPSVYHGDMNTYRQM
                     LDKEPMDAVIIATYWDSHTPIALHALKNGIYPGIEVPAALTVDDTWNLVETSENTGVP
                     CMMLENWSFRQDNLALLNMVRLGMFGEIVHCHCAHSHDCIDHWFFDSKTGEQRWGAEY
                     LLRYNRDQYPTHSVGPVISWLDINRGDIFTEITSTATASKGINAYFKRRFGDSHPNAN
                     LKYSQGDIVTSVLKTKMGKTLVINYDMQLPRPYSNRWMLQGTKGVYFEEKPAIYLVDV
                     SPEYHQWEPWKPYEQKFNHKWWQGDYSALSHGGTDYVMLSQFISAVRAKGQTPVDVYD
                     SAVMTAIVELSGISIANNAPVAFPDFTKGKWQSNKPYFAMDRTL"
     gene            3943..5055
                     /locus_tag="PK288_02970"
     CDS             3943..5055
                     /locus_tag="PK288_02970"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17910.1"
                     /translation="MRYVFLITTVAASLFLSGCGSTNKSDEPEAIKAYCIDYNWGPGG
                     PNGFAAPGLWADADPAEHVKWYADLGCNVIQSFAVSCNGYAWYKNGIVPEQPGLKHDF
                     LSEQVKLAHEKNIKVFAYFCAGSNTRWGMENPDYSYGIPALPHIPYTRKYLDFLCESI
                     RDAIIKTGIDGVFIDWLWNPGAVMEPYPPLKWIPCEQEMYQELMGEPFPGIDNVTPER
                     ELEFRRKAIDRAWKRIRETVKNTDPGCIIWLTCAQLTSRDIAGSDMLKEVDWVLNEAG
                     DTATTASSRSMLGPETRMVTCLANWNGQDAAIVIPDAIKKSIGLYGFTKPVNGSAMPP
                     VDHYLSVHPDTLEGDARNIALLARSYNNLPFDYVKK"
     gene            5189..7090
                     /locus_tag="PK288_02975"
     CDS             5189..7090
                     /locus_tag="PK288_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPM17911.1"
                     /translation="MNTIIRTITFFVIIIFLSHCRRISDTNESDSGLKVIPKPVSTTL
                     LPGEFRLRKHTVIHFDDDSASAVAEYLAVRIRDISGYEIRTTQGNGRGINLTIVEDLQ
                     KSVGDEGYKLSVKPGEIVISAPKRNGLFYGIQTLLQMIPVKEENCAGVSSDIIRIPCA
                     EITDFPRFKWRGLLLDVSRHFFTTDEVKRMIDQMVRYKFNVLQLHLTDDQGWRIEIKS
                     LPGLTSTGAWRVPRTGLWWDRECPQEGEAPTYGGYYTHEQIKDLVKYAAFRNVDILPE
                     IEAPGHSLAAIASYPHLSSTGIQYRVNPGCRFYTIEDNSICPGKESSYDYMDKVLTEV
                     AQLFPFEYIHIGGDECYKGFWMKCPDCRKKMRENGLKTYEELQSYFIRRMEKIIESKG
                     KKLIGWDEILEGGLAPNAAVMSWRGMQGGITAARSQHHVVMSPVDYAYLDFYQGDPAI
                     EPPTYAMLRLKKVYEFEPVPEGVDPEYILGGQGNLWTESVPTFRHAEYMLWPRAFALS
                     EVLWTPAGERQWNDFVKRTEYNLKRLDQSGINYARSFYDAIIIPSRDDNGNLLIALDS
                     EVDDIDLYYTFDNTFPDDHSPAYINGERLTIPKDADTFRVISYRDGKPIGKMITVSLT
                     DLAKRIPTG"
     gene            7422..10010
                     /locus_tag="PK288_02980"
     CDS             7422..10010
                     /locus_tag="PK288_02980"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HPM17912.1"
                     /translation="MLMIIAGCGDKSVSNISTSEAYTKYERKTESKSLVAFSESRENP
                     LKWFDSIPERRLLDAFEGRQLKLTANPGEIFVYQIGVWATNMDLNDLQISFSDLKGKA
                     GKISSSKMTCYNLGGTDIRGIAFRKQLSIPGGRVQALWIGMDLDSVVSGTYRGSVTVV
                     SGSGKQVLPVTLKVTGPRVLNNGYDEGARLSRLNWLNSRKGINNEVTKGFIPVKVEGN
                     SLSILGRNLAIGGNGLPASIVSFFKSSNQHIDEKGEYVVNSPFRFIIEKQHGGVISLV
                     PGEIVFSDKSDAGISWKVTNSSEEADLEVSGRLEFDGFVDYAMKLTAKRTLKIRDIRL
                     EIPVVKDKAEYMMGLGHEGGLRIHGWKWKWDVSKNQDMLWIGSVNGGIRLKLKAENYV
                     RPLVNVYYKFCPLKMPVSWGNGGNGGVDITDGETGVVIKAYSGGRTVETGEALNYDFE
                     LLLTPFRTIDRNTKFNDRYYHGGGASTTFKIDSAKKYGANIINIHHAEDIYPFINYPY
                     LDANVAELKRLVSEAHDNGIRMKLYYTTRELTKNLPEFPAFYSLDGEIIFPGPGNDSR
                     TVINPKGPNEWLVKNLREKYIPAWYNEIEEGKFRGETDLSVITTPDSRLNNFYVAGLE
                     WMVKNIGIDGVYIDDSALDRFTVQRARKVIDEYRPEGRMDLHSWNHFNEWAGYTNCLN
                     LYMDMLPYFDLIWIGEGRDYNRDPDHWLIEVSGIPFGLPGQMLEGGGNPWRGMVYGIT
                     NRAGWVKNPPHYLWKFFDDYKFSEREMLGYWDRNCPVRTDNPKIIASAFKGSEDLVFA
                     IANWSDKEQACRLIIDRKALETDLSSTVLEVPFIENFQESGSVTPGDQIMIEGGKGRL
                     LVVKTK"
     gene            10248..11594
                     /locus_tag="PK288_02985"
     CDS             10248..11594
                     /locus_tag="PK288_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HPM17913.1"
                     /translation="MISKTSASSRPQQAMWLTIGTIGLFFFIFGFVTWLNGILIPYLK
                     ISCELNHFQSYLVAFSFYLAYVIMAIPASWVLKRTGLKTGMMIGLFIMAGGALFFVPA
                     AATRTYGIFLTGLFVMGTGLALLQTASNPYITILGPIESAARRISIMGICNKSAGIIA
                     TLVFGVMALSDGDALVAQLKTLEGPEKNLMLDQLAARVIVPYLGMAAILFAIGLMIKL
                     SRLPEIDPNENNAGNIKDEFSNRKSIFQFPFLLLGVVAIFCDVGLEIIAGDTIISYGI
                     SQGIPIALARHFTSFTMIASIIGYLVGIITIPKYISQHNALKICAILGTVLTIAAIVS
                     PRMISVLFIASLGMANALIWPAVWPLAIEGLGRFTGIGSSLLIMGNLGGALLPLLYGR
                     LADIMDPRTAYFILIPCYFFVLYYASRGYKVGRYNPRHDIHALDENLLVKSDNFIK"
     gene            11591..12808
                     /locus_tag="PK288_02990"
     CDS             11591..12808
                     /locus_tag="PK288_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM17914.1"
                     /translation="MNKELSKDPVRIGVVGTGKRGQKLIGYLLQLDDVEITAVCDGSV
                     SEAEIAAGMCTSSGKKKPSVFGGKKDSYRRALDSGLLDAVIIATYWDSHAEIALLAME
                     NGIYPGIDVPAAQTVEQAWKLVETSEKTGIPCMMLENWSFRKDNLAVLNMKRLGMFGE
                     IIHCHCAHSHNCIDHWFFDSETGEPKWQAEYLMKHNRDQYPTHAVGPVISWMDINRGD
                     IFTEIFTMTSASRGINAYFADKFGADHPNAVTRFRQGDIVTSLLRTSSGKTLVVNNDL
                     LLPRPYSNRWMLQGTKGIYDEEKSSIYLAGHSPQYQKWEPWQPYEDKYNHRWYQQDLS
                     SLRGRRADFVMLRRFVDAVAVRGPVPLDVYDSAVMSAIVELSEISARENRPVPFPDFT
                     RGRWKTNEPYFAV"
     gene            12829..13908
                     /locus_tag="PK288_02995"
     CDS             12829..13908
                     /locus_tag="PK288_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPM17915.1"
                     /translation="MIKIQASVSKGWLHKHGGFDFTDRYYLDPYFRMKQDEACHNFIK
                     KRFPDYPLYNMEDNLVQAEYVVPDMMLVGAIQPNMIIAAALGSEFVFAPDRDSDVRGF
                     PLKDIKDLKELPPVAEIPELEFILNLINQFLEAKEKYPWCRIIPPFFWDTSGRATIHG
                     IITTSMKFIGQDIFTMMMIYPDLVKGIHAWIADVYIEVIKLFSGLGDLPVTSVHVGEC
                     SGTMLDGASYLEFVTPYINRLGREFGAIRLHSCGNADHVMPSICAIDNLSVIDTGSDT
                     SLARIREMKGRDFEINVFPHVDYLSESARHKDIQDWLARTLKDNDGGNLKIEYHLESD
                     YNLENCMYIHDELDRLGLVKKGRLY"
     gene            14116..15138
                     /locus_tag="PK288_03000"
     CDS             14116..15138
                     /locus_tag="PK288_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPM17916.1"
                     /translation="MNGYQRIVAALKGERPDKIPVMLHNFMVAAEELNISMGRFRESP
                     TLIAEAFINSVEKYDLDGVLVDIDTVTLAGSLGVPVDLPEHEPGRSPGGCLASMEDVR
                     SLKPVNVEDYKYIQIWLEAVRRLKDYFGDEVFVRGNCDQLPFSLAGMMRGTGNWMMDL
                     YVAEHSLLSELLEYCTEATSQFIRLMIQTGADMVSNGDSPAGPDLVPPEIYEKYAFPY
                     EKRIVDEAHRGGAFYTLHICGDTKPILEKMLLTGSDAFEIDYKTDAQFACDTLRDKAA
                     FFGNVDPSGVLALGTPELVRKKTEELLRIFSDNNRFILNAGCALPSTTPEENLRTFVA
                     TARNFR"
     gene            15365..16774
                     /locus_tag="PK288_03005"
     CDS             15365..16774
                     /locus_tag="PK288_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM17917.1"
                     /translation="MTKRREFIKKSVLGSAGIAVGATGFSAKSYNSIAGSNERINLAV
                     IGIRNQGSVHIDSWCSLAKSHNVFLKTLCDTDEQLFGSRIKMVMDKAGHKPVTEWDLR
                     KVLDDKEIHAVSLAVPNHWHALATIWACQAGKHVYVEKPASHNIREGRKMVEAARKTG
                     LMVQCGLNNRSSSNVREAIKFLHDGGIGELFMARALCFKARDSYGMARDSKPPAGFHY
                     DMWLGPAPWRPYNVKRSHYVWHWYWDTGNGDTGNTGPHMLDIARWGLNKNEHPVSVYS
                     TGGIYGLNRDETRPENRTKGVMVYGDVEAYGRDKTTQETPNTQTCAYKYADGTLLEFE
                     CRGRYTNHEGSDGSEVTNVFYGTEGWLQIMGDTWKAFRGRERTPFAGSKPVTETSPAP
                     NYANFIDAIRADNIDVLNCEIYEGFLSSTLPHLANISYRVGRSLRFIGESEKFADDSE
                     ADALLTRIYRVPYVVPDTV"
     gene            17181..18890
                     /locus_tag="PK288_03010"
     CDS             17181..18890
                     /locus_tag="PK288_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HPM17918.1"
                     /translation="MNLTMIKKFFTGQAVYSNYLILSIFIFSLLVAGCQSGNKPARQA
                     KSAPLVAPPANDDFFQEISREIGLDFVHWIGGDELINIVMSDGGGAAFLDFDQDGYMD
                     IYVCTGTWIENFSKSKKPDVLPVNRLYRNLGNGTFEDVTRKAGVGGPWYTMGVSVADY
                     NNDGYPDLYLSNYGPNVLLKNNGNGKFTDVTKRAGVAGGNECSIGATWLDFDNDGYLD
                     VYVGNFLEFDPEYKYYYAPDGFPGPMAYDAQKDVLYRNNGDGTFEDVTDKMGIVDLDG
                     RAMGVGSADIDDDGWVDIYVANDHTMNYLWHNEGGKGFTDWGIRSGTAFSQGGEAAVS
                     MSVDYADFNDDGLLDMFVSDDTYCALYENLGNGLFADRGVPSGISMASAQFVGWSSSF
                     LDYDNDSYVDIFKVNGELKHLYGHEDQVFRNEGNGKFRDVSLELGEYFEQELVGRGAA
                     MGDYDNDGDIDIFIVNLNDHCAFLRNNKGNQNNWITLKLTGTTSNRDAVGARIRVTAG
                     GKTQTAVRKSTTGYLSQNDPRVHFGLAKSEIIDRIEIRWPSGKIQVLENISPNQILDV
                     KEP"
     gene            19002..20336
                     /locus_tag="PK288_03015"
     CDS             19002..20336
                     /locus_tag="PK288_03015"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17919.1"
                     /translation="MIILTESPELLRSPDYSEGTVWRYLPGARITAVNPDKPEKIIVL
                     TEEFSSACFPEISYDGKCMLFTARKNENDSWQIWEMNLRNLKSRKITSFDENCTDPVY
                     LPGNRMAFSRQTINDTVGNAHCIFTCRLDGSGVQQVTFSPASSFANTVLKDGRIITVN
                     TVLNPQEADPVLQIMRPDGTKADLYYKAPAGCRLISSVSETNDRHLIFAERNAGNPGK
                     DELISISYNRPLHTRINLTSSIDGDFLFALPVATDRFLVSYRKPGSESYGICEFDPSE
                     KKLGRQIYSDPRYNVVDAVIAVRHDRPKNLPSEVDMHVKTGLIMCQNINVFNAELPRS
                     FHETHKARRIEVVGVDTTYGVVDVEEDGSFYLKVIADTPFRIKTIDDNGNLISGPCSW
                     LWLRPNERRGCVGCHEDPELVPRNLLSIAVTKDPVIIPVHIGEIKEKIVELE"
     gene            20358..22406
                     /locus_tag="PK288_03020"
     CDS             20358..22406
                     /locus_tag="PK288_03020"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HPM17920.1"
                     /translation="MKKIYWKLVLIIGVIGLLIILIYPPFHRWARAHVNYQTAVEDHP
                     LNCLSCHLYTQKTGLISKLINADYYSPFNLALSNDGKRLYVVAEESNTLLVVDTEKRK
                     VTNKIKVGYRPHTVILDKDEKYGYVSNQWEDNIYVIDLGNNIVIDTLDTGNGPAGIAL
                     SSDGKFIYAVNSYTNDISVIDLSSGREWKRLAAGNNPTGIGMSPDGRRLLVTSRRANL
                     VPYGDPVVCELTVIDEDTKMVIDRQDMNSAYMLENIAFTPDGELALIPLIRPKNLVPS
                     IQVEKGFMMTNGMAVIEQKPDGRVIQLLLDEPNEYFADPFDVAITPDGKRAFVSHAGV
                     NRISVIDMDSIRSFISHTPTEMLEYYSNHLGISSKYLVKRIVTGANPKGLVLSSDGRY
                     LYVAEHLEDRIAVISTESLETVNTIDLGGPKRITVARQGRRILNNASGTFQTQYACYT
                     CHPDAHEDGLVYNMASKDMGRNLANTQSLRDIGDTPPFKWNGKNQTIYKQDGMRFSTV
                     LTRNEAFNYKDLDALVAYIVTGIPYPPNLLYNPTGELNAMQLRGKALFERTIDNHGNE
                     IPEKQRCITCHPPPYFTNKNFEDVGSLLASDDSMLFDTPHLNNIYASPPYLHHGRALT
                     LEEIWTVYAEEDTHGAVNDFTKIELNELIEYLKSIREPEYYINESKKNKVLQAKIKNH
                     "
     gene            22418..23614
                     /locus_tag="PK288_03025"
     CDS             22418..23614
                     /locus_tag="PK288_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17921.1"
                     /translation="MKRIFSNKLFRYSLLVLIIAGAGVLGLSKSRKPNSGAMKPKKAE
                     MADFSTIESREKEARPGNISEGIPIYGNWKTFTEKDGLPSDKCYAVRIDKERNRVLVG
                     THHGLAVYQDGKWTTYTKEDGLAHNGIVSIDISVLTGDVWIGTLGGLNRWSGGKFETF
                     TQFNSGMPNDLVYNVFCYGKDVWCATGGGAGHYDTQTRQWEIFTEMNAPMHEPWTYAL
                     SAGDGKVWIAAWGGGVIEYNTVTKQFRDYVDPDGFMEIDLQPDDGVVHDITTATSYSD
                     GILWISSYFGMSRYDGKNWKGYFDHDSGIASNFINFLRAERNVVFVCCDEGLSCTDGT
                     TWVTYHKFDNSTDGKAIVKEGNTLIKEVPLSPSISHNFIIGVDAEDDVLWVATSKGVC
                     RGELLN"
     gene            23711..25021
                     /locus_tag="PK288_03030"
     CDS             23711..25021
                     /locus_tag="PK288_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPM17922.1"
                     /translation="MKNLRVIFTGLLVSVSISMASGQQLPVNYGNIPDELVAFDKYQK
                     AYKYHFIEPMEFYGAGRELPFPTDLKEVRIGFLGPLEGSIISNLGQQMLQGSVLAIEE
                     ANKKGGYKGIPFKLMIHNDVGLWGAAANELVKMDDEGVWAWLGSIDDIVSHVAIRATL
                     KLEIPNINTGDPDPTFTETNIPWVLRTIPDDRQSSYALVDKIFVKDKHTRVAVIRANN
                     RYGRVGILHFNRTSTRIGYPLIIEERFMDNETDFRPQLNRIKKTSPDAIQIWGNAKES
                     ALILQQIRELGMNQPVYASDRVVSKEFLEIAGSLAENIITTCQYNPEANDPKLKAFKA
                     NYVKRFGQEPDVFAAHAYDGMNIIIEAIRKAGLNRVLIRDLLTDLKTFQGYQGVTGPI
                     HFDGTWNNIRPIFMAQVKNGKFEFYPAPAYQKDNTIYKKTSMGY"
     gene            25039..25926
                     /gene="aroF"
                     /locus_tag="PK288_03035"
     CDS             25039..25926
                     /gene="aroF"
                     /locus_tag="PK288_03035"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPM17923.1"
                     /translation="MMISNQSGISGTTGGCSVPIRVNRNTRTDPTMVNVAGIMFGPNE
                     IGIIAGPCAVESKEQLIETAKKVRNSGGAVLRGGAFKPRSSPYSFQGLGEEGVKLLCE
                     ARKETGLPFVTEVMDTRQVEYIAEHADMLQVGSRNMHNYPLLKEVGMTKKPVLLKRGM
                     MATIEELLHSAEYILNQGNDQVILCERGIRTFETSTRNTLDLNAVPMLKHLSHLPVIV
                     DPSHGTGHRWMVPAMARAAIAAGADGLIMEIHYNPDKALCDGHQSLSPEEFDRLMNDL
                     VKIARSIGREILLPQPSII"
     gene            26006..27073
                     /locus_tag="PK288_03040"
     CDS             26006..27073
                     /locus_tag="PK288_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPM17924.1"
                     /translation="MVYPGLYSQENPGNTIKIGLLIPERESTAALHGAQMAVNAANGT
                     GGPDGRNFSLVVRSMEGPWGTGSKQAVDLIFNEKVWALLGSHDGRNAHLVEQAATKSI
                     VTMLSAWSADPTLTQAFVPWFFNCVPNDLQQAETIINETFDKRKQKNLVVIYDQDYDS
                     RQALTSLLREMKGRNKPSPVQLSYEAYSSDPGSLPKRITQSNADCIALFCSPKVASGL
                     YALLRKSKINLPVFGSLSVLNEDQLTESELNKFEGSFYVTSANWNSEKCRNFIRDYRE
                     KYGSRPGLVAAYAYDATGLIIEAIRRAGSADRTKIQKALAELTYSGVTGTIRFDERGN
                     RSGNYNMVKIRNGLPAEFATD"
     gene            27202..30891
                     /locus_tag="PK288_03045"
     CDS             27202..30891
                     /locus_tag="PK288_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPM17925.1"
                     /translation="MEVCEKKVLRQHAMNLPVKAAGCFSFFRGRNYKILSMLLFLTAV
                     CQSCNYGQTKDQAKSQELISVRTLGLAYLEEFKLAEAEKEFLKFIKLAPKEKLGYANL
                     GLTYLRMGKYPEAEKQLIKAIKIDPKDPDIRLILATVYRMTDSRDKAIAELQEGLKSS
                     PAHVKTLYDISELYSLGTDKASAEQRELYTRKLVGSAPANVVPRLNLIDILIRKGNYD
                     EVISHLEILKKQYPEFSREAAEYYNKTMTALRSPSKENALNSFIIFHNYLKISAPYQA
                     GMVDLKGPGGSLVGFPLISFDEDSFTQTVASKAGAAVVKFTNATSSAGLDIVNSARKS
                     GSSGAKSHAFVVVSDYDGDGDMDLYVAITDPGSQISRHYLLNNELGRFKDVASRAGIK
                     HTGNETSAIFADYDNDGYQDLLIVRDEGNIMYRNNGKGGFDNTTDKTKLGDKPADRSK
                     GNIPLFVDLDHDGDLDLFVGRTSANRIYRNNADGTFLEQASAMGFDSANENTRGAVFG
                     DFDEDGDIDIFMVNENAGNRLYLNQRNGKFIESAERSGLKGEGASSSVAAGDYDNDGY
                     LDLFVTSVKPGNHVLYRNLRDGTFEKDKRPAALLKELGKTIVHDARFIDFDNDGMLDI
                     IVAGESSEKDGRGVFLFRNEGNGTFTNVSNILPADLKSGRQIAIMDYNDDGDLDILLA
                     GTDGTISLLRNDGGNAGHFINMKLVGLRTGSAKNNFFGIGAKVELRSGDLYQTMVVTQ
                     PNIHFGIGTKSRADVIRITWTNGVPQNIFFPETDQSFIETQMLKGSCPFLYTWNGNEF
                     VFAKDIMWRSGLGMPLGIMGGTTTYGFADASDDYIKIPGELLREKDGMYPIQITAELW
                     ETMYIDKVELVAVDHPESVDIFVDEKFSGPPFPELKIYTVGEKHFPVSATDSEGNDVL
                     SCITGDDDVYLGGFNPEKYQGITEMRDLILDLGEFDPGTGCYLFLKGWIFPTDASINF
                     ALSQTESLKVINPSLEVVDKNGNWTMAIENIGFPMGKDKTVIANLTGKFPTSDHRIRI
                     RTNMEIYWDQIFWSDCNVNYPLKTYFMDPVKADLHYRGFSRGFRKGGRYGPHWFDYSV
                     VDTTWKWRDLTGYYTRFGDVLPLLTGADNKYIITNAGDETSVWFSAEGLPAPEKGWKR
                     DFLIRSVGWVKDGDMNTAHGSTVLPLPFHGMKSYPPGETDVYPNDPDLEKYNREYNTR
                     RIDREDYLKAFRNKNFGLQGIRISE"
     gene            30925..32586
                     /locus_tag="PK288_03050"
     CDS             30925..32586
                     /locus_tag="PK288_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HPM17926.1"
                     /translation="MKKIFASLAAAVIAVLLFVVFKPGEKILTYTDVTGPAGIDFRYN
                     FGDYTYQSIIESSGSGITVFDYNNDGLMDIYMMNGTYLEGISDPAGSVFRDTPNKLYK
                     NNGDGTFTEVGHEAGVDDRNWSMAACAIDYDNDGDEDLFLLNYGPNVFFRNNGDGTFT
                     DITDQTGLRGPEKLNGFEKWSIGAVFFDYNGDNRLDVMVGNFLAFDPSIVSPVSREAQ
                     PHPSQYKGQASMLYEQQPDGSFRDVTKQVNLYYPDSKCMGLTVFDFDNDGVLDIFQAN
                     DHHFNYMFKSVDGKYQEIAIARGLAANSKGIGAGSMHGTIGDINGDGLPDILVSDTDY
                     GALYLNLGNGMFQDVTHSSGIASNLAGKGTWGTALFDFDNDGDLDIFAANGNAEELIL
                     QLQLLLENDGKGHFTDVGRRHSDYFLKRRSGRGMAVCDYDNDGDLDIIVSHVDLIATP
                     VLLRNDGGNRNNWLGLTLKSHAGLAPAISAKVTVFSGSLKQVLINQWITGYLSSNDPR
                     LHIGLGKHKTIDSLEIAWSDGQTETYRNIRANRYITIMQGEGVVE"
BASE COUNT         9589 a         7108 c         8199 g         8447 t
ORIGIN      
        1 tgggaatcat aattatgata ttttttggaa ttatatcata atatattgat tcccagggtt
       61 atgatcaaat attatacatt taatttattg acaatctgta ttttatatgt taattactta
      121 ttttaagtca actcttaatt cgcattatta aatttgaaat atcggaagcc gatagctttc
      181 tgaatgggat ggaagggaat ggaggctgat acggaggaat cagtttgtgg aagtcaccgg
      241 tcccgggaga ttatacggaa acaaaaatcc ggaaaccgat ccaatatggg ccaccggttg
      301 ggataataaa tcactgatcc tgggggttag ggataaaggt aaatggagct tctaccgcat
      361 gcccaaagca agccatacat acgacggtgc acacggatgg aacacggaat ggccccggat
      421 aaggaatgtt ggggaacggg acagagatga tctatgctca gatctctgtg gctgaaggag
      481 aagtgtttag gtacgttttc ccggagtgtt ttcaggctcg gtggatacga tttgtgcctg
      541 acaaaaactg caaggctaca gcatggctta aatacgaata aaaatgaaaa ctgacaccaa
      601 acaaatccgg gtatccggaa caatcacctt ctgcctgatc ttatttctga gtgttttcag
      661 tctgaaaggt cagaataatg atgaaatgcc tctgaaagtg ggcatggtat cagacattca
      721 gtatgccgat atcgacaatt ccggtacacg tcattacagg gcttctgtag gaaaattttc
      781 tgaggctgtc agtaaattca gggaagaaga tgtagacatg attgtcagcc ttggtgattt
      841 tattgacaat gattttaaaa gttacaaacc cctgatcaaa attaccaggg gaataaacat
      901 cccagtttat tttgtgcttg gcaatcatga tttttcagta cgggaaaact tcaagagaaa
      961 aatacctggt atgctgaatg tcagatcaag atattactct ttaaccaggg ataactggag
     1021 atttattttt ctcgacggta ctgatatcag taccttctca cggaggaaaa aagatcctga
     1081 gtataacagg gcgcgatcaa tccttgattc actaaaatcg gtcggagctc ccaatgcaca
     1141 ttcatggaac ggtggaattg gaaaagcaca gattgaatgg ttggagcagg agatcgacaa
     1201 ttcacataaa gcaggtcaga atatttttat attttgtcat tttcctgtat acccggaaga
     1261 ccagtcagag aacctctgga atgcagataa gatcagaaaa atagttgaat ccgggaacgg
     1321 gaaaattgtc ttcatgaccg gacataccca caggagcaac tactgtttta ataatggtgt
     1381 tcattatgtt tcgctcagag gcatggtcga gacggacgaa aactcctttg caatcgctga
     1441 aatatacgac gattcgataa tgatcaaggg ctatggtgct gaaaaggaca ccgtgataaa
     1501 atggtaattg atgatttaaa tattctgtat aatagagaga atctctcatt tcgctgattc
     1561 tacgcaggat aaagatcgtc tcattccccc agccggcctg atgtaccgtg tcattccgaa
     1621 tcccgggaaa tggttagagt aatctctttt atgaaccggt taacccatga cagttcacaa
     1681 gcttcaatat ctttcaaatg ccacaggaaa taggaatctc cttcagcatg acggtatact
     1741 cattgaggct ctccagtatt acagggaggg atttcatcca gatcatttta caggtttgcc
     1801 ggaatggaac catcaggcca tacagccctg agggctggaa gcccaatatc catggtcaca
     1861 tccatggtag ggcagtgcgg tatgctgtca acgccggggg cgttgaataa cacctcgtaa
     1921 ttcagatctg atattcagct ccttcaaagc tgaggcattt ttgaggtcgt tatccgcggg
     1981 attgtaccgc tattgttaat attgtgccac ttcacggcac tggctgcgca aaaatatgcg
     2041 gcttaagagc tatttccggg attactcaat tcgaagggag atccttaatt tggatgatcc
     2101 tttgcatgaa aaggatcgtt tctggggaag tatttttgtt ctcagcctga cgacagggag
     2161 atccctcatt cccccttagc caaccagatg taccgtgtca ttccgatcac accttagcgt
     2221 gagaggaatc tccttcagca gtacggtttg atataatagt tgtaaagtgt tattggtaat
     2281 ccaataaaaa tggataaggg atctccatct ttgtggtaat gttcaatcat cttgcatgaa
     2341 taatgatttt tcatcttaaa tgttgttaaa actgacaatt caattcaaat cggcactcaa
     2401 taaaaatgat atgactccgg gatattttaa aattagagaa caatatctca tttttggtgg
     2461 gcactaattt ttaaatgcct aattttaata ggatcttaga ataattttaa ccttatacta
     2521 taatgaaacc agttacaagg aggaatttca tcaggaccgg cgctgccgcc gcattgggca
     2581 taagtgccgt tccggctgcc gcttttgcgg ctgatgttga tgcacaaccg gttaaagttg
     2641 gtgtcgtagg tatgggcaac cggggtacga gccacgttaa ctgccttttg acgataggag
     2701 gagtcgagat cgtggcatta tgcgatcttt ttgaaaacag ggccgtcaat gctgcggaaa
     2761 tctgcgtgaa agcaggaagg aaacggccct ctgtttatca tggtgacatg aacacctaca
     2821 ggcagatgct tgacaaagag ccaatggacg cagtaataat tgccacctac tgggattcac
     2881 acactccgat agccctgcat gcattgaaaa acggcattta tcccggaatt gaagttcctg
     2941 cagcacttac cgtcgacgat acctggaacc ttgtggaaac atcagaaaat accggtgtcc
     3001 cttgcatgat gctggaaaac tggagcttca gacaggataa cctggcattg ctgaacatgg
     3061 tgcggttagg aatgttcggg gaaatagtcc attgccattg cgcacattca cacgattgca
     3121 ttgatcactg gttcttcgac agcaaaaccg gtgagcagcg ctggggagcg gaataccttc
     3181 tcagatacaa cagggaccag tatcccaccc attcggtggg acctgtcatc agctggctgg
     3241 atatcaacag gggagatatt tttacagaaa taacctcaac agccacggca tcaaaaggca
     3301 tcaatgcata tttcaaaaga agattcgggg acagtcatcc caatgcaaac ctcaaataca
     3361 gtcagggtga tattgtaaca tcggtcctta agaccaaaat gggaaaaacc cttgttataa
     3421 attacgatat gcagctgcca aggccctatt ccaacaggtg gatgctccag ggaacaaagg
     3481 gagtctattt tgaggaaaag ccggcgattt acctcgttga tgtaagtccc gagtaccatc
     3541 agtgggagcc atggaaaccc tacgaacaga agttcaatca caaatggtgg cagggtgact
     3601 attcagccct ttcgcacggc ggcaccgatt atgtcatgct cagccagttt atcagcgccg
     3661 tgagggcaaa gggacaaaca ccggtagatg tttatgactc ggcagtgatg acagccattg
     3721 ttgaactttc gggaatctcg atagccaaca atgctcctgt cgcatttccc gacttcacca
     3781 aaggaaagtg gcagtcgaac aaaccttatt ttgctatgga cagaacactt taaagggaaa
     3841 tattaattta atactcagcc agccttgtta tctgcgtaaa tctcaggatc agcgggaaaa
     3901 acattgttaa atgtggtaaa caacattcaa aaagaactcc tgatgcgata cgtcttttta
     3961 attacaacag ttgcagcttc actatttctt tcaggttgcg gcagcacaaa caaaagcgat
     4021 gagcctgagg ccataaaggc ttactgcatt gattacaact gggggcctgg cggtccgaac
     4081 ggttttgcag ctccggggct gtgggctgat gccgatcctg cagaacatgt aaagtggtac
     4141 gcagacctcg gatgcaatgt aatccagtca tttgcagtct cctgcaatgg ttatgcgtgg
     4201 tataagaatg gaattgtacc agaacagccc ggactgaagc atgatttcct ttcagaacag
     4261 gtaaaactcg ctcatgaaaa aaatattaag gtattcgcat atttttgcgc aggatccaat
     4321 acccgctggg gaatggaaaa ccccgattac agctatggca tacctgcatt gcctcacatc
     4381 ccttacaccc ggaaatacct cgacttcctg tgcgaatcaa tcagggatgc aataataaag
     4441 acgggcatcg atggcgtatt tatcgactgg ctctggaatc ccggtgccgt tatggagcct
     4501 tatcctccgc tcaaatggat cccatgcgaa caggaaatgt atcaggagct tatgggagag
     4561 ccattcccgg ggattgataa tgtaacacct gaaagggaac ttgaatttcg tcgcaaagcc
     4621 atcgatcgcg cctggaagag gataagggaa accgttaaaa atactgatcc cggctgtatt
     4681 atctggctta cctgtgcaca gcttaccagc agggatatcg cgggatccga tatgctgaaa
     4741 gaggttgact gggtgcttaa tgaggcaggc gataccgcca caacggcatc atcccggtca
     4801 atgctcggcc cggaaacaag aatggttacc tgcctggcca actggaacgg tcaggatgca
     4861 gcaattgtca tccccgatgc cattaagaaa tccattgggc tttacggatt cacaaaacct
     4921 gtgaatggtt cagctatgcc acctgtagac cactatctgt cggtacatcc cgacactctt
     4981 gaaggtgatg cccgaaacat tgccctgctt gcacgttcgt acaataatct gccatttgat
     5041 tatgtgaaaa agtaggcgac agatgccggg aaagatccgg gcagggcgga aaaatagcag
     5101 gtatttgtat ccgcacagga tccggtcaat tgtacaaatg gaacagagaa agctattttc
     5161 aatgttattt aatttcaaat cgagatttat gaacacgatc atcagaacaa ttacattctt
     5221 cgtgattatc atttttctgt cacattgccg aagaatttca gacacgaatg agagtgatag
     5281 cgggctcaag gttattccaa aacctgtaag caccacactt ttaccggggg aattccggct
     5341 caggaagcat accgttatcc atttcgatga cgattctgca tctgccgtgg cagaatatct
     5401 ggctgtccgg ataagagata tttcgggcta tgagatccgt acgacgcagg gaaacggtag
     5461 gggaataaat ctgaccatag tggaagatct tcagaaatcg gtgggcgatg aaggatacaa
     5521 gcttagtgtg aagccaggtg agatagtgat ctcagctcca aaaagaaatg gtctgttcta
     5581 tggtattcag actctccttc agatgatccc cgtcaaggaa gagaattgtg caggagtcag
     5641 tagtgatatt atcaggatcc cttgtgcaga aatcactgat tttccaagat tcaaatggag
     5701 agggcttctt cttgatgtca gccggcattt ctttaccacc gatgaagtta aaaggatgat
     5761 tgatcagatg gtcaggtaca agttcaatgt tcttcagctt catctcacag atgaccaggg
     5821 ctggaggatt gagatcaaaa gccttcccgg actgacctca accggtgcat ggagagtacc
     5881 gcgtaccgga ctatggtggg atcgtgaatg tccgcaggaa ggagaagctc ctacatacgg
     5941 aggctattac actcatgaac agattaaaga tctcgtgaaa tatgcagctt tcaggaatgt
     6001 ggacatactt cccgagatcg aagctcccgg gcacagcctt gctgcaattg cttcttatcc
     6061 gcatctttca tcaacgggaa ttcagtatag ggtaaatccg ggctgcaggt tctatacaat
     6121 cgaggacaac tcaatctgcc cgggaaaaga gtcttcatac gattacatgg ataaggtgct
     6181 cacagaggtg gctcagctct ttcccttcga atatatacac ataggcggag acgaatgtta
     6241 caagggcttc tggatgaagt gcccggactg ccggaagaaa atgagggaaa acgggctcaa
     6301 gacatatgag gagctgcaga gctattttat caggaggatg gaaaaaataa tcgaatcgaa
     6361 gggaaaaaaa cttataggtt gggatgaaat acttgaaggc ggacttgctc cgaatgctgc
     6421 tgttatgagc tggaggggca tgcagggagg cattactgcg gcaaggtcgc agcatcacgt
     6481 ggtaatgtcc ccggttgatt atgcatatct cgatttctac cagggcgatc ctgcaattga
     6541 acctcctacc tatgcaatgc tgcgtcttaa aaaggtttat gaattcgagc cggtgcctga
     6601 aggtgttgat ccggagtata ttctgggagg acagggtaat ctttggaccg aaagcgttcc
     6661 cacattccgt catgccgaat acatgctatg gccgcgggcc tttgccctgt ccgaagtgct
     6721 ttggacgcct gccggtgaac ggcagtggaa cgatttcgta aaacgcaccg aatacaatct
     6781 aaaacggctc gatcagtcag gtataaacta tgcaaggagc ttttacgatg ctataattat
     6841 tccctccagg gatgacaatg gaaacctgtt gattgcactt gattcagaag tagatgacat
     6901 cgatctttat tataccttcg acaatacttt tcccgatgat cattctcctg catacattaa
     6961 cggagaaaga ctcacgatac caaaggatgc tgatactttc agggttattt catacaggga
     7021 tggaaagccg atcggaaaga tgattacggt gtcactgacc gatctggcaa aacgtattcc
     7081 taccggataa ttgctattta acctgggtta ttcatttaca ttgtgatcct cgtgtgtact
     7141 taattcccct ttggggtaaa acaaagtcat tatttttata actgagtcac gaagggcagt
     7201 ataagtaaaa aattcataat attccggtgt aaaatctatc aggaagattc attcattttc
     7261 aaaacctgaa cccgatatta aatcggcgaa aaagaaaagg tatatttgtg cggcaggaga
     7321 cctgctgcta cagcgttcgg taatattctc atttatctgt tttagtcatg gatgcaatac
     7381 tgctgtttcg taaaacccgg aaaatcattg ttctaatcac tatgttgatg ataattgccg
     7441 gatgcggtga caagtcagtg agtaatatat caacatcaga agcttatact aaatatgaaa
     7501 ggaagacaga aagcaaatca ttagttgctt tttcagagtc cagggagaac cctttgaaat
     7561 ggtttgattc cattcctgag agaagattac ttgacgcttt tgaaggcagg cagcttaagc
     7621 tgactgccaa tcccggtgag atttttgtgt atcagatcgg agtctgggca accaacatgg
     7681 atttaaatga tctgcagatc agtttttcag atctgaaggg aaaggccggg aaaatcagtt
     7741 cttcaaaaat gacctgttat aatctgggag gtacagatat ccggggaatt gctttcagaa
     7801 agcaattaag tattccgggg ggccgagtac aggccctgtg gataggtatg gatcttgaca
     7861 gtgttgtttc aggaacatac aggggatcag tcactgttgt ttcaggaagc gggaaacagg
     7921 ttttgcctgt cacactcaaa gtaacgggtc cccgggtttt aaataatggc tatgatgaag
     7981 gtgccaggct ttcccggctc aactggctaa attcccgtaa aggaataaac aatgaggtta
     8041 caaaaggctt catcccggtt aaagttgagg gcaacagttt aagcatcctc ggccgtaatc
     8101 ttgctatcgg cggcaatggc cttccggctt caattgtcag tttttttaaa tcctcaaatc
     8161 aacatataga tgagaagggt gagtatgttg tcaattctcc tttcagattc ataattgaaa
     8221 aacaacatgg tggagtcatc agccttgttc ccggtgagat tgttttttct gataaatccg
     8281 atgccggtat aagctggaaa gtcaccaact catcggaaga ggctgacctt gaggtttccg
     8341 ggcgcctcga atttgacgga tttgtggatt atgccatgaa gctgactgca aaaagaacac
     8401 tgaaaataag ggatatccgc ctggagattc ctgtagttaa ggataaagca gagtatatga
     8461 tgggcctggg ccatgaaggg ggactgcgta ttcacggatg gaagtggaaa tgggatgttt
     8521 caaaaaacca ggacatgctg tggatcggat cagtgaacgg cggaataagg ttaaaactta
     8581 aagcggagaa ttatgtcagg cctctggtaa atgtatatta taaattctgc cctctcaaaa
     8641 tgccggtatc gtgggggaac ggaggcaatg ggggtgttga tattaccgat ggggaaaccg
     8701 gcgtcgtaat aaaagcatac tcgggaggac gtacggtcga aacaggagag gcccttaatt
     8761 atgactttga gctgcttctt acccctttca ggaccatcga caggaatact aaattcaacg
     8821 accggtacta tcacggaggc ggggcatcaa ccaccttcaa gatcgacagc gcaaagaaat
     8881 atggtgcgaa cattataaat attcatcatg ctgaggatat ctatcctttc ataaactatc
     8941 cgtacctcga tgcaaatgtt gcggaactga aaaggcttgt gtcagaggcc catgataacg
     9001 gcatcaggat gaagttatac tatacgacaa gggaactgac caaaaacctg ccggaattcc
     9061 cggcctttta cagcctggat ggtgaaatca ttttcccggg gcccggcaat gattccagaa
     9121 ccgtgatcaa tcccaaagga cccaatgaat ggctggttaa aaacctgagg gagaaatata
     9181 tccctgcctg gtataatgag atagaagagg gtaaattcag gggagaaact gatctttcgg
     9241 taatcacaac tcccgacagc aggctgaata atttttatgt ggcaggtctg gaatggatgg
     9301 taaagaatat cggcatcgac ggtgtttata ttgacgattc ggcccttgac cggtttacag
     9361 tgcagagggc ccggaaagtg attgatgagt atcgtcctga gggaagaatg gatcttcaca
     9421 gctggaacca tttcaacgaa tgggccggat atacaaattg cctcaacctg tacatggata
     9481 tgcttcccta tttcgacctg atctggatag gggaaggccg tgactataac cgggatcccg
     9541 atcactggct gatagaggtt tccggtattc ccttcggcct gccgggccag atgcttgagg
     9601 gaggcggcaa tccctggaga ggcatggttt acggtattac caaccgtgcc ggatgggtga
     9661 aaaatccacc tcactacctt tggaaattct ttgatgatta taaattttca gaaagggaaa
     9721 tgctcggtta ctgggacagg aactgtcctg taagaacaga taatccgaaa atcattgcat
     9781 cagctttcaa aggatctgag gatcttgttt ttgcgatagc caattggtct gataaagaac
     9841 aggcatgcag gcttatcatc gaccggaaag cattggaaac agatctcagc tcaactgtcc
     9901 tggaagttcc ttttattgaa aattttcagg aatccgggag cgttaccccc ggtgatcaga
     9961 taatgataga gggtgggaag ggtcgtcttt tggtcgtgaa aaccaaataa atattaatta
    10021 attaggtgat atacatcagt agatcgatag agatatatca ttaaatataa tgacatatat
    10081 caattactgc taatggcgac aattgtcaaa tattcttcgt aaatagttcc cggttttcaa
    10141 acggatcact tttttcttat ttttaaaaaa cttaaaccac aatactcaat cgggaatggc
    10201 cctgaatcct gcgttgcaac agtcatcaac aattttatga gcattatatg atttcaaaga
    10261 catccgcctc gtcccgtcca cagcaggcta tgtggctgac aattggtacc atcggcctgt
    10321 ttttctttat tttcgggttt gttacctggc ttaacggcat actgataccc taccttaaga
    10381 tatcgtgtga acttaatcat ttccagtcat acctggtggc cttttcattt tacctggcct
    10441 acgtgataat ggcaatacct gcctcgtggg tactgaaaag gacaggtctc aagaccggca
    10501 tgatgatcgg tctgttcata atggcgggtg gagcattgtt ttttgtacct gcagccgcga
    10561 cccggacata tggtatattc ctgacgggcc tttttgtgat ggggaccggc ctggcccttc
    10621 tgcagacagc gtcgaatccc tacataacca tactggggcc cattgaaagc gccgcccggc
    10681 gaatcagcat aatggggatc tgcaataaat ccgcagggat aatagccact cttgttttcg
    10741 gggtaatggc gcttagtgac ggagatgcac tggtagccca gcttaaaacg cttgaaggac
    10801 cggagaagaa cctgatgctc gatcagcttg cagctagggt catagtccca tacctgggaa
    10861 tggcagcgat cctgtttgcc atcgggctaa tgattaaatt atcccgcctt cccgagattg
    10921 atccgaatga aaacaatgcc gggaacatta aggatgagtt cagcaacagg aaatcgatct
    10981 ttcagttccc gttccttctg ctcggagttg tagcaatttt ctgcgacgtg ggtctcgaaa
    11041 tcattgcagg cgatacaata atcagttacg ggataagcca gggtatcccg attgcacttg
    11101 cccggcattt tacctcattt acaatgattg cttccattat aggatacctg gtgggaataa
    11161 tcacaatacc aaaatacatt tcgcagcaca atgctctgaa aatatgtgca atcctgggaa
    11221 cggtactgac cattgctgcc attgtatcac ccaggatgat ctcggtactt ttcattgctt
    11281 cactgggcat ggccaatgct ttgatatggc cggcggtgtg gccgctggca atagaggggc
    11341 tcggacgatt tacaggaata ggctcatccc tgcttatcat gggaaacctg ggcggtgcgc
    11401 ttcttccact gctttatggc cggcttgctg atatcatgga tccgcgaact gcctatttca
    11461 ttctgatccc atgctacttc tttgtgcttt attatgcctc aaggggttat aaggttggca
    11521 ggtataatcc ccgccatgat attcacgcgt tggatgaaaa tcttttggtt aaatcagata
    11581 atttcatcaa gtgaataagg aattgtcaaa agatcccgtt cggatcggcg tggttgggac
    11641 gggcaaacgg ggacagaaac tgattggtta tcttctgcag ctggacgatg ttgaaataac
    11701 tgcagtttgt gacggatcag tttccgaggc ggagattgcg gcagggatgt gcaccagttc
    11761 agggaaaaaa aagccctcgg tattcggcgg aaagaaggat tcatacagaa gggcgctgga
    11821 ttcgggctta ctggatgctg taataattgc cacctattgg gattcgcatg ctgagattgc
    11881 cctgctggca atggaaaatg gcatatatcc cggcattgat gttcctgctg cccagacggt
    11941 tgaacaggca tggaaactgg tggaaacatc ggagaagacc ggtattccat gtatgatgct
    12001 tgaaaactgg agtttcagga aagataatct tgcagtactg aacatgaaaa ggctgggtat
    12061 gttcggcgaa attatacact gccattgtgc ccattctcat aactgtatag atcactggtt
    12121 ttttgacagt gaaaccgggg aaccgaaatg gcaggctgaa tacctgatga aacataacag
    12181 ggatcaatat ccaacccatg ccgtggggcc tgtaatcagc tggatggaca tcaaccgggg
    12241 cgatattttt accgagattt tcaccatgac ttccgcttcc aggggtatca acgcctactt
    12301 tgcggataaa ttcggcgcgg atcatcccaa tgccgttaca aggttccggc agggcgacat
    12361 tgtaacttcc ctgttgcgta ccagctcggg aaaaacactt gtggttaaca atgatcttct
    12421 gctgcccaga ccctattcaa accggtggat gctccagggc acgaagggga tttatgatga
    12481 ggagaaaagt tccatatatc tggccggcca cagtccccaa taccagaaat gggagccgtg
    12541 gcagccatat gaagataaat acaatcacag gtggtatcag caggatcttt cttcactccg
    12601 gggaagaaga gctgattttg tgatgctccg caggtttgtt gacgctgtag cggtcagggg
    12661 gccggtaccg ctcgatgttt atgattcagc cgtaatgtcc gctattgttg agctttcgga
    12721 aatatcggca agagaaaacc gccccgtacc ctttcctgat tttacccgcg gcagatggaa
    12781 aaccaatgaa ccttatttcg ctgtttgacc aataaaaaat ttaagatcat gatcaagatt
    12841 caggcaagtg tttcaaaagg ctggctgcac aagcatggag gatttgattt caccgacagg
    12901 tactatctgg atccgtattt caggatgaaa caggatgagg catgtcataa ctttataaag
    12961 aaaaggttcc ctgattatcc tctgtacaat atggaagaca atcttgtcca ggcggaatat
    13021 gtggtaccgg acatgatgct ggtaggtgca atccagccga atatgataat tgcggcagcg
    13081 ctgggatctg aattcgtttt cgcacccgac agggattcgg atgtgagggg atttccgctg
    13141 aaagatataa aggacctgaa ggaacttccc cctgtagctg aaataccgga actggagttt
    13201 attctcaacc tgataaacca gtttcttgag gcgaaggaaa aatatccctg gtgcaggata
    13261 attccacctt tcttctggga tacctctggc agggcaacga tacatggaat aatcaccaca
    13321 tcgatgaaat tcatcgggca ggatattttt accatgatga tgatataccc cgatcttgtc
    13381 aagggaattc atgcctggat cgcggatgtt tacatcgagg tcataaaact cttttcaggg
    13441 cttggcgatc tgcctgtgac atccgttcat gtgggagagt gctcgggtac gatgctcgac
    13501 ggtgcatctt acctggagtt tgtcacccca tatattaacc gcctcgggag ggagtttgga
    13561 gccatcaggc ttcacagctg cggaaatgct gatcatgtaa tgccttccat ttgtgccatt
    13621 gacaacctca gcgttattga tacaggttca gacacctcac ttgcccggat cagggagatg
    13681 aaaggcaggg atttcgagat caatgttttt ccccatgtcg attacctttc cgaaagtgcc
    13741 aggcataaag atattcagga ttggcttgca cgcacactga aggataacga cgggggtaat
    13801 ctgaagatcg aataccacct ggaatccgat tacaatcttg aaaactgcat gtatatacat
    13861 gatgaactcg acaggctggg actggttaag aagggccggc tttactgaat tatgaagccc
    13921 gcatgatacg aaatcacaaa caagcatgaa aataaaattc atgtgggttc catgtgaacc
    13981 acttaatctt cagtagtata taaaattatt ttcacatgaa aatagtatcg gttgtgtgtt
    14041 actgaattga actgacggaa ataaaagacg gaataaatga atttcaggag taacgtgtgg
    14101 aaaaaataaa caaagatgaa cggatatcag aggatagttg ctgcactaaa gggagaacgg
    14161 ccggacaaaa ttccggtgat gctgcataat ttcatggttg cagccgagga actgaacatc
    14221 agcatgggca gattcaggga aagtccgacc ctgattgccg aggctttcat taattcagtg
    14281 gaaaaatatg accttgacgg agttcttgtc gacattgata ctgtgactct tgccggcagc
    14341 ctgggtgttc ctgtggatct ccctgagcat gaacccggaa gatcacccgg gggatgcctg
    14401 gcatcgatgg aggatgtcag atcactgaaa cccgtgaatg tagaggatta caaatatatc
    14461 cagatatggc tcgaagcggt aagaaggctt aaggattatt tcggcgatga ggttttcgtc
    14521 aggggcaact gcgatcagct gcctttttca cttgcaggaa tgatgcgagg gacaggcaac
    14581 tggatgatgg atctgtatgt tgccgaacat tctcttttga gtgaattgct tgaatactgc
    14641 actgaagcaa caagccagtt cataaggttg atgatccaga cgggagcgga tatggtatca
    14701 aacggcgaca gtccggccgg tcccgacctg gtgccgcctg aaatttatga aaaatatgcc
    14761 ttcccgtatg agaaaaggat tgttgatgag gcccacaggg gaggagcctt ctacaccctg
    14821 catatctgcg gcgacaccaa acccatcctc gaaaaaatgc tcctgaccgg ttcagatgca
    14881 tttgaaattg actacaagac agatgcgcaa ttcgcatgcg ataccctcag ggataaagct
    14941 gccttctttg gaaatgtcga tcccagcggt gtcctggccc tgggaacgcc tgagcttgtt
    15001 cgaaaaaaaa cggaagaact tctccggata ttttcggata acaacaggtt tatcctgaat
    15061 gcgggctgtg cactgccgtc aacaacacct gaagagaatc tcagaacatt cgtggcaacg
    15121 gcaagaaatt tcagatgaga aaccgggcag cataatttcc catatctgcg gtgaccgtga
    15181 ccggagcagg tctgatctgc ccgagaggcc cggcagggtt cattctgatt actgcaatac
    15241 ttatcaggtt gctgaataga aattaatgta ggtgaggact gataaataag aaattgtttt
    15301 taacttacta tcctgaaaaa tcaagccgga attacctgaa aaaataattt aaatcatctg
    15361 aaaaatgaca aaacggagag aattcatcaa gaaaagtgtt ctgggaagtg ccgggatagc
    15421 cgtcggcgcc actgggtttt ctgcaaaatc ctataattcc attgccggtt caaacgaaag
    15481 gatcaatctt gccgtgatag gtatcaggaa ccagggaagc gtgcatattg acagctggtg
    15541 cagcctggct aaaagccata acgtgtttct gaaaactctt tgcgacaccg atgagcagct
    15601 tttcggatca cgaattaaga tggtaatgga taaggccggt cataaaccgg ttacagaatg
    15661 ggatcttaga aaggttctgg atgataagga aattcatgct gtttcgctgg ctgtgccaaa
    15721 tcactggcat gcgcttgcaa caatatgggc ttgccaggca ggcaagcatg tctacgttga
    15781 gaaacctgca tcacacaata tccgcgaagg gcggaaaatg gtggaggctg cgcggaaaac
    15841 cggacttatg gtccaatgcg gactgaataa ccgttcctcg tcaaatgtaa gggaggcaat
    15901 caaattcctt catgatggcg gtatcggtga gcttttcatg gccagggcac tttgcttcaa
    15961 ggccagggat tcatacggga tggccaggga cagcaaacca cctgccggct ttcactatga
    16021 tatgtggctc ggccctgccc cctggcgtcc gtataacgtc aaaagaagcc actatgtatg
    16081 gcactggtac tgggatacag ggaacggcga cacgggaaac accgggcccc atatgctcga
    16141 tatagcccgt tgggggctga ataagaacga acatcctgtt tcagtttatt ccacaggagg
    16201 tatttatggc ctgaacaggg atgaaaccag acccgaaaac cggacgaaag gagtgatggt
    16261 ctatggagat gttgaagcct atggcaggga taaaaccact caggaaactc ccaacaccca
    16321 gacctgcgca tacaagtatg ccgacggcac tctcctggaa tttgaatgca ggggacgtta
    16381 caccaatcat gaaggatctg acggatcgga ggttacaaat gttttttacg gaacagaagg
    16441 atggctgcag atcatggggg atacatggaa ggctttccgc ggaagggaac ggaccccttt
    16501 tgcaggatca aaaccagtga ctgaaaccag tcctgcaccc aattatgcaa acttcattga
    16561 tgcgatcagg gcagacaaca ttgatgtcct taactgcgaa atctatgaag ggtttctttc
    16621 ctcaaccctg ccccatcttg ccaacatatc ataccgggtt ggcagaagcc tgaggtttat
    16681 aggcgaatcg gaaaaatttg cggatgattc ggaagctgat gcccttctta caaggatata
    16741 cagggttccc tatgtggttc cggatactgt ttaacaattt ggcaggacac gcatgccgga
    16801 taggccgcaa tgccagcccc acgggaaaaa ccccgtactg gtacgtcttg tcaacatgtt
    16861 gtaccggatt gtttcactat taacgactaa ttgttaaaat cacttcggga gttaacaaat
    16921 actgcctgaa attatttccc atgtccgtta caaattttcc ggaaacatga tctgcattcc
    16981 ccggaaaaga caggcaagtg aactttttta acattttaaa tcccctttaa atggggattt
    17041 aatcttgaaa atgttaaaat attttatatt ttgttaacta atcatgcatt aatgttaaat
    17101 tattattaaa gatgtgttaa atgagtatgg cttaaaacaa aaggatgaca tatattacat
    17161 aatttcgtta aacctaacct atgaatctaa ccatgataaa gaaattcttt actgggcagg
    17221 ctgtctattc taactatctt attcttagta tttttatttt ttcgctgttg gttgccggat
    17281 gtcagtcggg caataaacct gcacgacagg caaaaagtgc gccccttgta gcaccccctg
    17341 ccaatgatga ttttttccag gagatatcaa gggagatagg actcgacttt gtccattgga
    17401 tcggaggaga tgagcttatc aatatagtaa tgtccgacgg gggaggagct gcattcctgg
    17461 attttgacca ggacggttat atggatatat atgtctgtac cggaacgtgg attgagaatt
    17521 tcagcaagtc gaagaaaccc gatgttttgc ctgttaaccg gctttacagg aaccttggga
    17581 acgggacatt cgaggatgtc accaggaaag ccggtgtagg agggccatgg tacactatgg
    17641 gtgtgtcagt tgctgattac aacaatgacg gataccctga tctttacctg agtaattacg
    17701 gccccaatgt tcttctgaag aataacggca acggaaaatt taccgacgtt acaaagcgtg
    17761 ccggggtggc gggcggcaat gagtgcagca taggagcaac ctggctggat tttgacaatg
    17821 acggttacct tgatgtttat gttggcaatt tcctggaatt tgatccggag tacaaatatt
    17881 attatgcccc tgatggcttt cccgggccta tggcctatga cgcccagaag gacgttcttt
    17941 accgcaacaa cggtgacggg acgtttgaag atgtaactga taaaatggga atcgttgatt
    18001 tggacggcag agccatgggc gtgggatcgg ccgatatcga cgatgacggg tgggtcgaca
    18061 tttatgtggc caacgaccat acaatgaatt atctctggca caacgagggc gggaagggtt
    18121 tcacagactg gggaatcaga tctggaaccg cattcagcca ggggggtgag gcagctgtca
    18181 gcatgtcagt cgattatgct gatttcaacg acgacggcct cctggacatg tttgtgtcgg
    18241 atgataccta ttgtgccctt tatgagaatc tgggaaacgg actgtttgct gacaggggcg
    18301 tgccgtcggg tatctcaatg gcatctgcac agtttgttgg ctggtcatcc tctttcctcg
    18361 attatgacaa cgacagctac gttgatattt tcaaggttaa cggagagctt aagcatcttt
    18421 acggacatga ggaccaggtc ttcaggaatg agggaaacgg aaaattcagg gatgtttccc
    18481 tggagctggg tgaatatttt gaacaggagc tcgtcggacg tggtgcagcc atgggggatt
    18541 acgacaacga cggtgatatt gacatattca ttgttaatct taacgatcat tgtgctttcc
    18601 tgcgaaacaa caaggggaat cagaacaact ggataaccct gaaactgacg ggcacgacca
    18661 gcaacaggga tgctgttggc gcaaggatca gagtgacggc cggcggaaag acccagacag
    18721 cagtcagaaa aagtaccacc ggatatcttt cccagaatga tccgcgtgtc catttcggac
    18781 tggctaaaag tgaaattatc gaccggatcg aaatcaggtg gccttccggt aaaattcagg
    18841 tactggagaa tattagtcca aatcagattc ttgatgtgaa agagccttaa ataatttatt
    18901 atatgagtgg agtcgggttg aagtttttcc ggggatttcc tgcgttttta ataatttatg
    18961 ttttcctggt ttcctgcacg ggaaaatcag taggggatgg aatgatcata cttactgagt
    19021 ctcctgaatt attgcgatcg ccggattatt cagaagggac ggtgtggaga tatcttccgg
    19081 gcgcaagaat aactgccgtt aacccggata agccggaaaa aatcattgtg cttacagaag
    19141 aattcagttc ggcatgtttt cccgaaatat catacgatgg taaatgcatg ctctttactg
    19201 ccaggaaaaa tgaaaatgat tcatggcaga tatgggaaat gaacctccgg aatcttaaaa
    19261 gccgaaaaat cacctcattt gatgaaaact gcaccgaccc tgtctatctt cccggaaaca
    19321 ggatggcctt cagcagacaa accataaatg ataccgtcgg gaatgctcat tgcatattca
    19381 cctgccggct tgacggatcg ggagtgcagc aggtaacgtt cagtcccgcc tcaagctttg
    19441 caaatactgt actgaaagac ggccggatta ttacggtcaa tactgtcctg aacccgcagg
    19501 aagctgatcc tgttctgcag attatgagac ccgacggaac aaaggctgac ctgtattaca
    19561 aggctcctgc cggttgccgg ctaataagca gtgtaagtga aaccaatgac agacacctga
    19621 tatttgcgga aagaaatgcc ggaaatcctg ggaaggatga acttatttca ataagctata
    19681 atcgacccct tcatacaaga atcaatctta cttcatcaat tgacggtgac ttcctgtttg
    19741 cactgccggt tgcaactgac cgcttcctgg tttcttacag aaaacccgga tccgaatcat
    19801 acggaatctg cgagtttgat ccgtctgaga agaaacttgg ccggcagata tactccgacc
    19861 cgcgatacaa tgtagttgac gcagtgattg ctgtccggca tgacaggccc aaaaacctgc
    19921 ccagcgaggt ggatatgcat gttaagaccg gtttgatcat gtgccagaat atcaatgttt
    19981 tcaatgcaga gctgccacgg tcgttccatg aaacgcacaa agcccgcagg attgaagtgg
    20041 tcggggtgga tactacatac ggagtggttg atgttgagga agacggttct ttttacctta
    20101 aggtgatagc tgatactcct ttccggatta aaacaatcga tgataacgga aatttgatca
    20161 gcggtccctg ttcctggctg tggctcaggc caaatgaaag acggggatgt gtgggttgcc
    20221 atgaagaccc tgaactggtg ccccgaaacc tcctgtcaat tgcagttaca aaagatcctg
    20281 tcatcatacc tgttcacata ggtgagataa aagaaaaaat agttgagctg gagtgattta
    20341 acaaagaggt tggcaaaatg aaaaagattt actggaagct ggttttaatc attggtgtca
    20401 tcgggctgct cattattctg atataccctc ccttccacag gtgggccagg gcacatgtta
    20461 attatcagac tgcagttgag gatcaccccc taaattgcct gtcgtgtcat ctttatacac
    20521 agaaaacggg actgatctcc aaactcatta atgctgatta ttattctcct tttaatcttg
    20581 cattgtcaaa tgatggtaag agattgtatg ttgtggcgga ggaatccaat acattgcttg
    20641 tagttgatac agagaaaaga aaagtgacca ataagattaa ggtcggatac aggccccata
    20701 cggtgatact tgataaagat gaaaaatatg gctatgtgag caatcaatgg gaggataata
    20761 tttatgtgat tgatctcggg aataatattg taattgatac tcttgatacc ggaaatggtc
    20821 ctgccggaat tgcattgagt tctgatggca aattcattta tgcggtaaac tcttacacaa
    20881 atgatatttc cgtaatcgat ctgagttcag gaagggaatg gaaacgactc gccgccggta
    20941 ataacccgac ggggatcggg atgtcacctg acggcaggag attgcttgtg acaagcagga
    21001 gggccaatct tgttccttat ggagaccctg tggtctgtga actcacggta atcgatgagg
    21061 ataccaaaat ggttatcgac cggcaggata tgaactccgc ctatatgctt gaaaacatcg
    21121 cgtttacacc cgacggagaa cttgccctga tcccccttat ccggcccaaa aacctggtac
    21181 cgtcaatcca ggtggaaaag ggctttatga tgaccaacgg gatggccgtt attgaacaga
    21241 aaccggatgg aagagtaatc cagcttctac tcgatgagcc aaacgagtat tttgccgatc
    21301 ctttcgacgt ggccattaca cctgacggta aaagagcttt cgtgtcgcac gcaggggtaa
    21361 acaggatcag cgttattgac atggattcaa ttcgttcatt tatcagtcac accccgaccg
    21421 aaatgcttga gtactattcc aatcatcttg ggataagcag caaatacctg gtaaaacgta
    21481 ttgttacggg cgccaatccc aaaggactgg tcttatcatc cgacggcagg tatctctatg
    21541 tggccgaaca tcttgaggac aggatagcgg ttataagcac cgaaagcctg gaaacggtga
    21601 atacaatcga cctcggcgga ccgaagagaa tcacagttgc acgacaaggc cggcgaatac
    21661 tcaacaatgc cagcggtacc ttccagaccc agtacgcctg ctatacctgt catcctgacg
    21721 cacatgaaga cggactggtc tacaatatgg catccaagga catgggtcgc aaccttgcaa
    21781 atacacagtc gctgcgcgat atcggcgaca ctccgccatt caagtggaac gggaaaaacc
    21841 agacaatcta taaacaggac ggaatgagat tctcaaccgt gctgacgcgg aacgaggcct
    21901 tcaactataa ggatctcgat gccctggtag cttatatagt taccggcata ccctatcctc
    21961 cgaaccttct ttataatccg acaggtgaac ttaatgcaat gcagctaagg ggaaaggcat
    22021 tgtttgaacg caccatcgac aatcatggca acgagatacc cgaaaaacaa cggtgtatta
    22081 cctgtcatcc gcctccctac tttacaaata aaaatttcga agatgttgga tctctccttg
    22141 caagcgatga ttcgatgctg ttcgacactc cccacctgaa caacatttat gcatctccgc
    22201 cctatctcca ccacggccgt gcccttaccc tggaagaaat atggacggtg tatgcagagg
    22261 aagataccca cggagctgtt aatgatttca caaagatcga gcttaatgaa ctgattgagt
    22321 atctgaaatc aatccgtgaa ccggagtatt atataaatga aagcaagaaa aataaagttt
    22381 tgcaggcaaa aattaaaaat cattaatcct gacttccatg aaaagaattt ttagcaacaa
    22441 gttattcaga tacagtctgt tggtcttgat tattgccggc gctggcgtcc tgggcctaag
    22501 taaaagcagg aaaccaaact ccggagcaat gaaacccaaa aaggccgaaa tggcagattt
    22561 ttcaacgatt gaatcgcggg aaaaggaagc cagacccggt aatatttctg aaggaattcc
    22621 aatttacggc aactggaaga cattcactga aaaagacggg cttccatccg ataaatgcta
    22681 tgcggtgagg attgataagg agaggaaccg tgttcttgta ggcacccatc atggccttgc
    22741 ggtttaccag gacggaaagt ggaccaccta caccaaagag gacggccttg ctcataacgg
    22801 catagtttcg attgatataa gcgttctaac gggcgacgta tggatcggaa cgcttggtgg
    22861 ccttaacagg tggtcgggcg gtaagttcga gacatttacc cagtttaaca gcggtatgcc
    22921 gaatgatctg gtgtacaatg tgttctgtta cgggaaagat gtttggtgtg ccaccggggg
    22981 cggagcaggc cactatgaca cccagacccg ccagtgggag atcttcaccg agatgaatgc
    23041 tcccatgcac gaaccatgga catatgccct ttcggcaggt gatgggaaag tgtggatcgc
    23101 agcctggggc gggggcgtga tcgaatacaa cacagtgaca aaacagttcc gcgattatgt
    23161 cgaccctgat ggtttcatgg aaatagacct gcagcccgat gacggtgttg ttcatgacat
    23221 aaccaccgcc acatcctatt cggacggcat cctgtggata tcatcctatt tcggaatgag
    23281 ccgctatgac ggaaagaact ggaagggcta ttttgatcac gacagcggta ttgcaagcaa
    23341 tttcataaac ttcctcaggg ccgaacgaaa tgtggtattc gtttgctgtg atgagggact
    23401 gagctgtact gacggcacga catgggtaac ctaccacaag ttcgacaaca gcaccgatgg
    23461 aaaggcaata gttaaagagg gaaatacgct tataaaggaa gttcccctgt caccttcgat
    23521 atcacataac tttatcatag gggttgacgc cgaagatgat gtgctctggg ttgccacatc
    23581 caagggtgtg tgcaggggag agcttctgaa ttaaccccct gaacaggttg tgaatattgt
    23641 ttaattatat gatatatgta gtaatcaaac ccggaactga ataaaccttt aaaatcaaaa
    23701 ccacctccat atgaaaaatc tacgtgtaat tttcacaggt ttactggtct ccgtctcaat
    23761 atcaatggcg tcaggacaac aattgccggt aaattacggc aatattccgg atgagcttgt
    23821 agcattcgat aaataccaga aagcctataa ataccatttc atagagccaa tggagttcta
    23881 tggagcgggt cgtgagctgc cgtttccgac tgatctcaag gaagtgagga ttggattcct
    23941 gggccccctc gaaggatcca ttataagcaa tttgggtcag caaatgcttc agggatcggt
    24001 tctggcaatt gaagaggcaa ataaaaaagg cggttacaag ggaattccct ttaagcttat
    24061 gattcataat gacgtcggcc tctggggagc cgctgcaaat gaacttgtta aaatggacga
    24121 tgagggtgtc tgggcatggc tgggatcaat tgacgacata gtgtcgcatg ttgcaataag
    24181 ggcaaccctt aagcttgaaa ttcccaatat caatacaggc gaccctgatc ctactttcac
    24241 cgaaaccaat atcccctggg tactgcgaac cattcctgac gaccgtcaga gcagctacgc
    24301 ccttgtcgac aaaatattcg tgaaagacaa gcacacacgg gtggctgtaa ttcgcgccaa
    24361 taaccggtac gggcgggttg gtattcttca ttttaacagg acctctacac ggataggtta
    24421 tcccttgata atcgaagaac gtttcatgga caacgaaact gattttaggc cacagctgaa
    24481 caggataaaa aagacctcac ccgatgccat ccagatctgg gggaatgcaa aagagtcggc
    24541 actgatcctc cagcagatac gggaactggg catgaaccag cccgtctatg cttccgaccg
    24601 tgttgtaagc aaagagtttc ttgaaattgc cggaagcctt gccgaaaaca tcataacgac
    24661 ctgtcagtat aatcccgagg ccaatgatcc caagctgaaa gcatttaaag caaactatgt
    24721 taaaaggttc ggacaggagc ccgatgtttt tgctgctcat gcatatgatg gaatgaacat
    24781 tatcattgag gctataagaa aagcagggct gaacagggtg ttgatacgcg acttgctcac
    24841 cgatctgaaa acttttcagg gataccaggg ggttacaggt ccgatccatt ttgacggaac
    24901 ctggaacaac atacgaccca ttttcatggc gcaggtaaaa aatggcaaat tcgagtttta
    24961 tccggctcct gcataccaaa aggataatac gatctataag aaaaccagta tgggatatta
    25021 acagaaagga gggacgtaat gatgatatct aaccagtctg gcatttccgg aaccaccggg
    25081 ggttgctccg ttccgatcag ggtcaaccgg aacacacgga ctgatcctac catggtgaat
    25141 gtggcaggca ttatgttcgg acccaatgag atcggtatta ttgccggtcc gtgtgcagtt
    25201 gagagcaagg agcagcttat tgaaacggca aaaaaggtca ggaactcggg aggtgctgtg
    25261 ctgagaggcg gtgcattcaa accccgttcc tcaccataca gttttcaggg gctgggagaa
    25321 gagggagtaa agctgttgtg cgaggccagg aaggaaacgg gattgccttt cgtaaccgaa
    25381 gttatggata caaggcaggt tgaatacatt gctgaacatg ccgacatgct tcaggtagga
    25441 tcaaggaaca tgcacaatta tcccctgctg aaggaagtgg gaatgacaaa aaagcctgtc
    25501 ctcctcaaac ggggtatgat ggcaacaata gaggagttac tccattctgc cgaatacata
    25561 ctcaatcagg ggaacgatca ggtgatcctt tgcgagagag gaatccggac tttcgaaaca
    25621 tccacaagaa acacccttga ccttaatgca gtcccgatgc ttaagcatct gagccacctg
    25681 cctgtgattg tcgatccgag ccatggcacg ggtcacagat ggatggtgcc tgccatggcc
    25741 agggctgcca ttgcagccgg agcagacggc cttattatgg aaatacacta caatccggat
    25801 aaggccctgt gcgacggaca ccagtcacta agcccggagg agtttgacag actcatgaac
    25861 gatcttgtta aaattgcccg gagcattggc cgggagatcc ttctgcccca gccttcgatt
    25921 atttgagggt tgatatatcc ggcagaatat gtctgaaaat agatctataa taatccggac
    25981 cggtcttatt gttagtctct ttttaatggt atatccggga ctttattccc aggaaaatcc
    26041 gggaaatacc attaaaatcg gattgcttat acctgaaagg gaatcaacag cagcattgca
    26101 cggagcccag atggccgtaa acgcggccaa cggaacagga gggcctgacg gcaggaattt
    26161 cagcctggtg gtccgatcaa tggagggtcc ctggggaacg ggatcaaaac aggcagtgga
    26221 cctgatcttt aacgaaaaag tatgggccct gcttggatcc catgacggaa gaaacgccca
    26281 tcttgttgag caggcagcaa caaaatccat tgttaccatg ctatccgcat ggtcagccga
    26341 tccgaccctt acacaggcat ttgtcccatg gtttttcaat tgtgtcccta acgatctcca
    26401 gcaggctgaa acgatcataa atgaaacatt tgacaaaagg aaacagaaaa atctcgttgt
    26461 aatttacgac caggattatg actcccgcca ggcccttacc agcctgctgc gtgaaatgaa
    26521 aggcagaaac aagccctccc cggttcagct gagttatgaa gcatactcgt ctgatccggg
    26581 cagcctgccc aaaaggataa cacaatcaaa cgcagactgt attgccttgt tttgcagccc
    26641 aaaggtggca tccggcctgt acgccctgtt acggaaatcg aaaataaacc ttcctgtatt
    26701 cggatctttg tctgttctga atgaagacca gctgaccgaa tccgaactga ataaatttga
    26761 aggaagtttt tatgtcacct ctgcgaactg gaattctgag aaatgccgga atttcatcag
    26821 ggattacagg gaaaaatacg gcagccggcc aggtttggtc gcagcctatg cctacgacgc
    26881 cacgggcctg atcattgaag ctatcaggcg ggcaggctcg gctgatcgta cgaaaataca
    26941 aaaagctttg gccgaattaa cttattcggg agttaccggc acaatcagat tcgatgaaag
    27001 aggaaacagg tccggtaatt acaatatggt aaaaatcagg aacggtcttc ccgcagaatt
    27061 tgcaacagac tgaaatatcc ggatggaatc tgacgggaat gactgaaaag gttatatcgg
    27121 ggattggcgg agcgccggat tatcattacc tttaactttt gcttttacga aaaagtttca
    27181 tttagtcata aaataacagg aatggaagtt tgtgaaaaaa aagtattaag gcagcatgca
    27241 atgaacctgc cggttaaagc agcaggttgt ttctcttttt tccggggcag aaactataaa
    27301 atcctgtcaa tgctgctgtt cctgactgca gtctgtcaga gctgcaatta cggacagaca
    27361 aaggatcagg caaaatcgca ggaacttata tcagtcagaa cactcggtct tgcatatctt
    27421 gaagagttca agcttgcaga ggctgaaaag gaattcctga agtttataaa acttgcacct
    27481 aaggaaaaac tcggctatgc caacctcgga cttacctatc tccgaatggg taaatatcct
    27541 gaagctgaaa aacaactaat aaaggccata aaaattgatc cgaaggatcc tgacatcagg
    27601 ctgatactgg caaccgtata ccggatgact gattcacgcg acaaagctat tgcagagctc
    27661 caggaagggc ttaaatcatc tccagcccat gtgaaaacct tgtatgatat ctcagaatta
    27721 tactcacttg gaacggataa ggcttctgcc gaacagagag aactctatac ccgcaaactg
    27781 gtcggaagcg ccccggcaaa tgtggttccg aggctgaatc tgatagatat acttatcaga
    27841 aagggaaatt atgatgaggt tatctctcat ctcgagatcc tgaaaaaaca atatcccgaa
    27901 ttttccaggg aggccgcaga atattacaac aagacaatga ctgcactgag atcaccgtca
    27961 aaagagaatg cccttaattc tttcattata tttcacaact acctgaaaat atccgcccca
    28021 taccaggcag gaatggttga tctgaaaggt ccgggcggat cgcttgtggg ctttccgctc
    28081 atctcattcg atgaggatag ttttacacaa accgttgctt ctaaagcggg tgctgcagtc
    28141 gtgaaattta ccaatgccac ttcgtcggca ggtctggata tcgttaattc tgcccggaaa
    28201 tcaggttcat ccggtgcaaa atcccatgca tttgtggttg tcagcgacta tgacggtgac
    28261 ggggatatgg acctttatgt cgccatcacc gatcccggct cacagatatc aagacactat
    28321 cttctcaata atgaactcgg gaggtttaag gatgtagcat cgagggcagg cattaaacat
    28381 accgggaacg aaacttcggc gatctttgcg gattatgaca atgacgggta ccaggacctt
    28441 cttatagtaa gggatgaagg aaacatcatg tacaggaaca acggaaaggg cggcttcgat
    28501 aatacgactg ataagacaaa actaggcgat aagcctgcag acaggtcaaa aggaaacatt
    28561 cccctgtttg ttgaccttga tcacgacggc gacctggatc tgtttgtcgg ccggacctcc
    28621 gcaaacagga tatacaggaa taatgccgac ggtacattcc ttgaacaggc atcggcaatg
    28681 ggatttgatt cagcaaatga gaatacccgt ggtgctgttt tcggcgattt tgatgaagac
    28741 ggtgatatcg acatttttat ggtgaatgaa aatgccggga acagattata tttaaaccag
    28801 agaaacggga aattcattga gtcggccgaa cgcagcgggt tgaaaggtga aggcgcttca
    28861 tcctcggtgg ctgcaggtga ttatgacaat gacggctatc tcgacctctt tgtgacttct
    28921 gtgaaacccg ggaatcacgt tctttaccgg aacctgaggg acgggacttt cgaaaaagat
    28981 aagcggccag cagccttact gaaagaactg ggaaaaacaa ttgttcatga tgccaggttc
    29041 atcgatttcg ataatgatgg catgctggat atcattgttg caggggaatc ttcagaaaaa
    29101 gacggccggg gtgtattcct tttcaggaat gagggcaacg gcacttttac caacgtaagc
    29161 aatattcttc cggctgatct gaaatcgggc agacagattg caataatgga ttataatgac
    29221 gacggtgacc tggatatcct tctggccggc accgacggca ctatttccct ccttcgcaat
    29281 gacggcggga atgccgggca ttttataaac atgaaacttg tcgggctaag gacaggcagt
    29341 gcaaaaaata attttttcgg aatcggagcc aaggtggagc tgaggtcagg tgacctttat
    29401 cagacaatgg ttgtcactca gcccaatatc catttcggca ttggaacgaa atcgagggcg
    29461 gatgtaatac ggatcacctg gacaaacggg gtgccgcaga atattttctt tcccgaaacc
    29521 gatcagtcat ttatagaaac ccagatgctt aaaggctcct gtcctttcct ttatacatgg
    29581 aacggaaatg aatttgtttt tgccaaggat ataatgtggc gaagcggcct tggaatgccc
    29641 ctcggaataa tgggtggtac cacaacttac ggatttgccg atgcttccga cgactacatc
    29701 aagatacccg gcgaactgct aagggaaaag gacggcatgt atccgatcca gataactgcc
    29761 gaactctggg aaacaatgta tatcgacaag gttgagcttg ttgcagttga tcatcctgaa
    29821 tcagttgata ttttcgtgga tgagaaattt tcgggccctc ctttcccgga attgaaaata
    29881 tacaccgtag gtgaaaaaca ttttccggtt tctgcaactg attccgaggg gaacgacgtg
    29941 ctttcctgca tcaccggtga tgatgacgtt tatcttggcg gattcaatcc ggaaaaatac
    30001 cagggtatta ccgaaatgag ggatctgatc ctcgacctgg gagaatttga tccgggtacc
    30061 ggttgctatc ttttcctcaa gggctggatt ttcccgactg atgccagcat taatttcgca
    30121 ttgtcgcaga ctgaatccct gaaagtgatt aatccctcgc tcgaggttgt cgacaaaaac
    30181 gggaactgga ccatggccat cgaaaatata ggctttccga tgggtaagga caaaacggtc
    30241 attgccaacc ttacgggaaa attcccgacc agcgatcatc gtataaggat caggacaaac
    30301 atggaaatat actgggatca gatcttctgg tcagactgca atgtcaatta tccgttaaaa
    30361 acatatttca tggatccggt taaggcggat ctccattaca ggggattctc cagggggttc
    30421 aggaaaggcg gcagatacgg accccactgg tttgattact ccgtggttga cactacctgg
    30481 aaatggagag accttacggg ttattataca aggttcgggg acgtactgcc tttgcttacc
    30541 ggggcagaca acaaatatat aataacaaat gccggagacg aaacctcagt ctggttcagt
    30601 gccgaaggat tacctgcccc ggaaaaggga tggaaaagag atttccttat caggagcgtg
    30661 ggctgggtaa aagacggtga tatgaacacc gcacatggaa gcactgttct tcctctgcca
    30721 tttcatggca tgaagagcta tccgcccgga gaaacagatg tttatcccaa cgatcctgat
    30781 cttgagaagt ataatcgtga atacaatacg aggcggatcg atcgcgaaga ttatctcaag
    30841 gctttcagga acaaaaattt tggactccag ggaatcagga tttctgaata atcgggttat
    30901 atttgcattt ctcatttttt caggatgaaa aagatatttg catctttggc ggcagccgtt
    30961 atagcagtat tgctgttcgt ggtgttcaag cccggagaaa aaatactgac ctatactgat
    31021 gttaccggcc ccgcagggat cgacttcaga tataacttcg gggattatac ttaccagagc
    31081 ataattgaaa gcagcgggtc aggcataacc gtgtttgatt acaataacga cggcctgatg
    31141 gatatttata tgatgaacgg gacctatctc gaaggcatat ccgatcccgc gggaagtgta
    31201 ttcagggata cacccaacaa gttgtacaag aacaacggtg acggaacttt caccgaggtc
    31261 gggcatgagg ccggcgttga cgacaggaac tggagcatgg ccgcctgtgc catcgattat
    31321 gataatgacg gtgacgagga cctgttcctg cttaattacg ggcctaatgt ttttttcagg
    31381 aacaatggtg acgggacctt tactgatatc accgaccaga caggactcag gggcccagag
    31441 aaactgaatg gttttgaaaa atggagcata ggagcggtat ttttcgatta taatggagat
    31501 aacaggctcg atgtaatggt aggtaatttc ctggcatttg acccgtcaat tgtatcaccc
    31561 gtctcccgcg aggcccaacc tcatccgtca caatacaagg gtcaggcttc aatgctttat
    31621 gaacagcagc ccgacggatc cttccgtgat gtgaccaaac aggtgaacct gtattatccc
    31681 gattccaaat gcatgggact tactgtgttt gattttgaca atgacggcgt cctcgatatt
    31741 tttcaggcta atgatcatca tttcaattac atgttcaaat cggtcgatgg gaaataccag
    31801 gaaattgcga ttgcgagagg cctggcggca aacagcaagg ggattggcgc cggatcaatg
    31861 catggtacaa ttggtgatat aaatggtgac gggttacccg atattcttgt atctgacaca
    31921 gattacgggg cgctctatct caacctgggc aatggaatgt tccaggatgt gacccattca
    31981 agcggtattg caagcaatct ggcagggaaa ggtacctggg gtaccgccct ttttgatttt
    32041 gacaatgacg gcgatctcga tatattcgct gcaaacggga atgcagagga actgattctc
    32101 cagctgcagc ttcttctgga aaatgacgga aagggccatt ttacagatgt gggacgacga
    32161 cacagcgact atttcctcaa gcgccggtca gggcggggca tggcagtgtg cgattatgac
    32221 aatgacggtg acctggacat aatagtttca catgtcgatc tgattgccac gcctgtcctt
    32281 ttaaggaacg acgggggaaa caggaacaac tggctgggcc tgacactgaa aagtcatgcc
    32341 ggcctggctc cagctatcag tgcaaaagtg accgtttttt caggatcact taaacaggtc
    32401 ctgataaatc aatggataac cggttatctt tccagcaatg atccccgcct ccatataggt
    32461 ctcgggaaac acaaaacaat agattccctt gaaatagcct ggtcagacgg acaaaccgaa
    32521 acttacagga atatcagggc taaccgctat atcaccataa tgcagggtga gggagtcgtg
    32581 gagtgaaaaa tataacacat cacttcctta gacaccatga aaactgatgt ttgattaaat
    32641 cacctatacc cgctctgcat aaatcctcct gtttaatcag aaatgaatat ccaataagca
    32701 cggatccggg ctgacaaatg cagttaattc ccggcattcc ggtctcaacg gaaactgaaa
    32761 gatcaatgta ttgatttact gatttgatcg atatcagccg tggattctgc ggacaaatga
    32821 ccggatttcc ttatcaggcc tgtaaatcct gcctgaatgt aaaattcact gattaatgaa
    32881 taggtgtatc ttgttggacg tggatattac atacatggaa aagcaagagt tgggcaagat
    32941 tcattctgat cgggaaattc aataagacta tcggcatctg atgtgtttaa gcgtttgtaa
    33001 ttgagccatc aaaaaaaatc aaatgaggag aattccgtgc atgcgaaaag aggcatatcc
    33061 ggatattttt tcgaaggata tgcctgcagg gacatataac aaatcattct cttcataaga
    33121 ggattttttt gacagataaa attcagatat cccggaatat tattcttcag atgacggagg
    33181 aaaaagaatc tgtcgggtat gatgctttta tccgcaagaa ccggaaaccg atgaaaaaga
    33241 gactgcagtt tacttaaatt aatttaaccc caaaaaacct taaagattat gaaacgaaag
    33301 atccagttca ttccttttat tttgctgctg ttgcttttgc cgg
//
LOCUS       DAOOYG010000018        31316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1232_length_31316_cov_8.030421, whole genome shotgun sequence.
ACCESSION   DAOOYG010000018 DAOOYG010000000
VERSION     DAOOYG010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31316
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1232_length_31316_cov_8.030421"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PK288_03055"
     CDS             complement(<1..592)
                     /locus_tag="PK288_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17927.1"
                     /translation="MKRILFLIILVSLSAETIKSQCIALSSGPGTDNQTVCINTSIAA
                     IVYRVIPGVTGVTPTNLPPGVTGLFNPTALTYTITGTPATAGDYTYNLQTLGGCIGVI
                     SGSIRILPDQVITLTSPPTPTPQAVCVNTPITSIIYSVSGSATGATVTGLPAGVTGSY
                     NAGVFTISGTPTVSGVFNYTVTTTGQCKPATATGTIT"
     gene            complement(899..3079)
                     /locus_tag="PK288_03060"
     CDS             complement(899..3079)
                     /locus_tag="PK288_03060"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPM17928.1"
                     /translation="MDVKVFAATVIALMLPCKPVGAQEDLNVVSNNWLEFSDARNSLY
                     HYLSERAYELLEERASRIAGIRTLDQWQERQNFIRTKLNELIGPFPAKTDLNARVTRV
                     IGKTGYRIEHIIYESQPGFYVTSSLFIPAGVSKGKKAPAIIYCSGHTVEGYRSPVYIH
                     VIQNLVKKGFIVFAFDPVGQGERLEYYDPSTGKSMVGGPTKEHSYPGAQAFLTGSSQA
                     FHMIWDGIRAVDYLLTRKEVDPSRIGITGRSGGGTQSAYISAVDDRIYAAAPENYITN
                     YSRLFQSIGPQDAEQNIPRLIISGLDHPDFLIIRAPKPTLLITTTRDIFSIQGARETR
                     NEVSAIFESYGAGESFNMVEDDAPHESTVKNREAMYAFFQKFLNNPGSPADFETEVLT
                     EEELRVTAAGQVSESFAGETVYSLNLKRLSRLTAELNIRRKDPGSFYPEVIRSAERLS
                     GYIKPEKPLDPVFTGRIKKDDYSIEKYFIELKEGYIIPYLIFKPSVPGNRVMLFLDPE
                     GKNHAFGNDGSLEMIVKEGITILAPDLLGVGEMGSGDFKGDADFNGASHNLWYASILT
                     GRSILGIHAGDIARLVRLILKDNPEAEIIGFARGAMSPALLHAAAFNNEIRSIILEGP
                     YSSFRSIANCRFYLPLFILSTVSEALKEYDLPDLAASLAPRRLILIDPVDGNGKREHP
                     GEVNRDIDLIKAGFQYYKADSQLTVISSADNPSTLPDLLRAMDF"
     gene            3358..3972
                     /locus_tag="PK288_03065"
     CDS             3358..3972
                     /locus_tag="PK288_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17929.1"
                     /translation="MEKIKINMLRFAGVVVLGFGLRSFVVCQAPVQDILPKSTLRDQM
                     NYVQEKTRIYDNYRAIREDVFQQIKRNSLDSLQVSKTEITRLKVLAESKNREIDSLNS
                     TLSAREKELQEMTTFRNSIRLFGIEVDKRTYKGIMWLVIAILSIILISGFLSYKRNRI
                     VTHNTRKEFEDLKKEFEAYRKASREAREKMSMAHFNELKKLRTG"
     gene            complement(3998..5083)
                     /locus_tag="PK288_03070"
     CDS             complement(3998..5083)
                     /locus_tag="PK288_03070"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPM17930.1"
                     /translation="MKVSDLLRTFTVLFLAAFLVISCSSAGGDKNQAVDFIKKEAERK
                     VDVLIGGKLVTSFCWPVNVYKPIFYPVYTISGTEVTRGFPLRPRAGERNDHIHQVGIW
                     FNYGDVNGVDFWGNGSEGHKSESGGEIRLLKIDNVEGGNGKGTMNISASWQDPSGKEL
                     LSERTEFNFFARGKYYIIDRITVLKANDIPVSMKDTKEGMYAIRVARQLEMPSTDDDL
                     LLDQSGSPGAEKAGENIGVTGNYRSSEGKEGDAVWGTRARWMNLTGLINNDEISIVMI
                     DHPDNVSYPTYWHARGYGLFSANPLGAVDFTQGNERVDFSVPAGGEVIFRYRMVINSG
                     MHLTDDIINSIADEFAAEYRQEKGDGR"
     gene            complement(5232..6269)
                     /locus_tag="PK288_03075"
     CDS             complement(5232..6269)
                     /locus_tag="PK288_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM17931.1"
                     /translation="MERRNFIRSVAAGSVGLTMTGNGFSAGHLKGSSGKRIGIIGLDT
                     SHSVAFTQALNEPSAGPEFGGWKVVAAYPRGSNDIKSSVERIPGYTEDVKKLGVEITG
                     SIDELLRQVDAVLLETNDGRIHLEQALPVFRAGKRMFIDKPIAASLPDAIAIFNASGY
                     YRVPVFSSSSLRYLPGIKEIKNGSAGKVIGADTYSPATIEKTHPDLFWYGIHGVEALF
                     TVMGTGCKSVTRIFTEGTDLVTGLWNNERIGTFRGIRAGRGDYGGTAFCEKAVVDLGK
                     YSGYNPLLEQVVKFFDTGIPPVSQEETIEIFAFMAAADESRKNGGRPVEISTILQKAR
                     DDAGKIRIQGS"
     gene            complement(6318..7688)
                     /locus_tag="PK288_03080"
     CDS             complement(6318..7688)
                     /locus_tag="PK288_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM17932.1"
                     /translation="MTKRRDFIRKSAIGSAGMVIGGMGFGTKAYSSIIGSNERINIAV
                     IGIRGQGGGHINNWCTLKDNRNVRLKAICDVDEQYFEAAAKTILERTGEKVVTPWDMR
                     KIFDDRDIDAVSFATPNHWHALGTIWACQAGKHVYVEKPASHNIFEGRKMVEAARKYN
                     VRVQTGFQNRSIKNVVNAIRFLHEGGIGDVFMAKGLCYKPRDSFGIAPDSAAPASLHY
                     DMWLGPAPYRPYNEKRVHYNWHWFWDTGNGDTGNQGPHQFDIARWGLNKNEHPVSVFS
                     VGGLFGIDPSECAQETPNTQTSLFSYSDGKILEFETRGRYTNDEASLGIRIGNIFYGT
                     EGYLELDGSTWKAFRKRETEPFAGSAKDEPRVTETGYLAAPGGTEHYANFIDAIRSGR
                     DQDLNCPIVEGFLSSTLPSLANISYRLGRKLTFDGQREKFVNDREADLLLTRDYRKPY
                     VVSDVI"
     gene            complement(7725..8609)
                     /locus_tag="PK288_03085"
     CDS             complement(7725..8609)
                     /locus_tag="PK288_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPM17933.1"
                     /translation="MKNRMTRRSFVRNSVISGAGLAVLGSVQGCSGRNPGKMKFGLVT
                     YQWGRDWDLPTLIANCAEAGYEGVELRTQHAHGVETSLSSSRRLDVRKMFEDSPVKCV
                     GYGSNFEYHSPDPDQLRENIEQTKEYIKLCRDIGATGIKVKPNYLPPGIPREKTIAQI
                     AGSLNEVGRFARDFDQLIRVEVHGEITQELPVMKAIFDQVTEDNVKICWNCNDQDLLP
                     PGLEGNFNMVKKWFGDTIHVRELDSTDYPYQQLFDLLSGIRYEEWILLEARTEPADRV
                     GAMKEQLKIFNRLAGYSV"
     gene            complement(8958..9833)
                     /locus_tag="PK288_03090"
     CDS             complement(8958..9833)
                     /locus_tag="PK288_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPM17934.1"
                     /translation="MKKPSLFSLLVVLAFFGLVSLPFNSCREAPKKNIGLQLYSIRDS
                     IRRDVPGAIAKVAAMGYTFVEPAGYRDGQFYGMEPVAFRNLCATNGLGLISSHTGQVL
                     PDEASRETTMAWWDACIDAHAAAGVSYLVQPFMGASAYRSLDTLKMYCDYFNLIGEKC
                     RAKGIRFGYHNHDAEFSTQLDGTTVYDFMLENTDPSKVIFQLDLYWCVVGGKNPVDYF
                     NKYPGRFELWHIKDKEEVGASGMMDFQAIWAAAEKSGLKYGIVEVEEYNFDQFTSCAK
                     SLEFLNAANYVVMPR"
     gene            complement(9924..11144)
                     /locus_tag="PK288_03095"
     CDS             complement(9924..11144)
                     /locus_tag="PK288_03095"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17935.1"
                     /translation="MIPTKSRFQVDNRKIVIFGAGKIGRSFIGQLFGTAGYKVVFVDT
                     DRLLTALINERKCYRVVAKSEREETIVVRNVSAIDAADVPAVGEAVSTAGIVAVSVGK
                     NAVEKVVPMIADGLKLRHDRFPGQPLDIIIAENMISGRDFMEENLKKFLPPEFPLNEM
                     TGLVETSIGKMVPIMSQSDLEKDPLQVFAEPYNILILDKKGFRTPIPQIKGLAPKENI
                     KAWVDRKAFIHNLGHATAAYYGYYLHPESAYMYEVLNDAKVSGFTREVMMQAAEILIE
                     AYPGEFTATDLAEHIEDLLSRFRNRFLRDTVFRVGHDLGRKLGSGDRFMGAVMLAVRH
                     GKPYQKILKAMSYGFVFKATDESGSNFESDLQLIDALQSDFENTLVNLLRFNPESDSA
                     IIRDLKNYMNQISE"
     gene            complement(11113..12300)
                     /locus_tag="PK288_03100"
     CDS             complement(11113..12300)
                     /locus_tag="PK288_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPM17936.1"
                     /translation="MPKKIIVSGAGCCLVDLLYNDIDFTGQSIAPFLSEKRGDGGLVP
                     GNLVFTEEFEKFTGIPVGNAVGKITGGRKHDKISIGGPSIVSLINVTQIVDKHQCEVR
                     YYGLGGNDDMGRFLISSLGRTGIKLRNYRLIDRSTPSTIVLSDPLFEGGHGERIFINS
                     IGSAWEFGRDNLDDDFFSSHIVVFGGTALVPRLHDDLSVLLEKAKNNGCITVVNTVFD
                     FRSEKADPSRKWPLGKDDRSYGYIDLLIMDREEALRLSGENSIDDAVTYFESLKVSSI
                     IVTNGPRNITLLSDGRLFAHTRRMELPASARVIDELKQSGGGDTTGCGDNFAGGVIAS
                     LVNQMIRGTPQPDLVEACCWGIVSGGFACFYIGGTYFEEYEGEKLSRMKPYYDSYKEQ
                     ISG"
     gene            complement(12436..13110)
                     /locus_tag="PK288_03105"
     CDS             complement(12436..13110)
                     /locus_tag="PK288_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HPM17937.1"
                     /translation="MKRSEINSIIESFAGFLADNRFFLPQWASWGPADWKGKYNICSE
                     IVDNKLGWDITDFGSGDFFRTGLCLFTIRNGSWDKKDKLYCEKIMMAREEQETPMHFH
                     RNKSEDIINRGGGNLVMELYESTSGSKLSDAPLTVSIDGVLTTVNAGRALILRPGQSI
                     CLKPGVYHRFYGQKGKGSVLIGEVSLVNDDAGDNHFYEETGRFPEIIEDVMPSHLLVN
                     DYEKFL"
     gene            13404..14441
                     /gene="asnA"
                     /locus_tag="PK288_03110"
     CDS             13404..14441
                     /gene="asnA"
                     /locus_tag="PK288_03110"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HPM17938.1"
                     /translation="MIFHAATAQTATRTDVLLTEEMIDFVKSDFGNNLARNLNLVKVS
                     SPLVVPDGTGINDDLNGIEKPVRFPVKSLDNTNAVIIQSLAKWKRIRLEYFNIGAGRG
                     ILTDMRALRPDEKLSPIHSLYVDQWDWEKHIRPDQRTLNYLKSTIEDIYKTFRETEEK
                     LYRKYEWLVPQLPEKITFIHSEELLQQYPYLNPKQRENAIARVHGAVFIIGIGSKLSN
                     GEPHDGRAPDYDDWSSVNEDGYYGLNGDIIFWNQTLDSAFEISSMGIRVDKSSMKRQL
                     RLCGCQEREKLMFHSMLLNDQLPLSIGGGIGQSRLCMFFLRKSHIGEVQSSIWPDDIM
                     TGAMENGLKLL"
     gene            14775..16607
                     /gene="ilvD"
                     /locus_tag="PK288_03115"
     CDS             14775..16607
                     /gene="ilvD"
                     /locus_tag="PK288_03115"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPM17939.1"
                     /translation="MKTKINLRSSKTTTGRNMAGARALWRANGMKEDQFGKPVIAIVN
                     SFTQFVPGHVHLHDIGQHLKQQIEKSGFFAAEFNTIAIDDGIAMGHEGMLYSLPSREI
                     IADSVEYMCNAHQADAMICISNCDKITPGMLLATMRLNIPTVFVSGGPMEAGYNKGRF
                     LDLIDAMVAAADNSVTDDEIREIEMSACPTCGSCSGMFTANSMNCLVEAIGLGLPGNG
                     TIIATHTNRLRLFEKAASLLVDIVKKYYYEGDTSVLPRSIATRQAFMNAMALDIAMGG
                     STNTVLHLLAMANEAGVDFSMKDIDELSRKIPNICKVAPSSSYHVQDVNRAGGILSIM
                     GELDRNGLIDPDVRRIDYPTLRNALRDWDIMSEKPLDEALDVYHSAPSMRRNLVLASN
                     DARYEALDTDREKGCIRNIENAYNKEGGLAVLYGNIAPKGSILKTAGIDRSCFYFRGP
                     VVLFESQEDAVDGILGGKVKEGDAVVIRYEGPKGGPGMQEMLYPTSYLKSRLLDKKCA
                     LITDGRFSGGTSGLSIGHVSPEAAAGGAMALLRDGDMIEIDVNNRKMNVLLSDDELAA
                     RRRTEESKGEKAFRPATRNRTISASLRVYASMVSSADLGAVRII"
     gene            16680..18425
                     /gene="ilvB"
                     /locus_tag="PK288_03120"
     CDS             16680..18425
                     /gene="ilvB"
                     /locus_tag="PK288_03120"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPM17940.1"
                     /translation="MKTEKEPITVIEAKNGNGTISGAEALLKCLIEEGVDTIFGYPGG
                     AIMPVYDKMLDFSSNIRHYLSRHEQGAAHAAQAYAMVTGKPGVCFSTSGPGATNLVTG
                     IANAFLDSIPVVFITAQVASNLIGTDAFQETDILGISMPVVKWNAQVKKAQDIPETIA
                     KAFYIAKTGRPGPVLIDITKDAQVDTLNFTYKKCNYIRSYNPVIALNTRSVESAAIMI
                     NHAEKPLILAGHGILISGAEKELLQFAEKTGIPVAVTLLGLSAFPSGHRLFAGFLGMH
                     GNYGPNLLTNEADLIIAIGMRFDDRVTGNIKKYAKNARIIHIEIDEAEINKNVLVDVG
                     INSDARVALKHLTPLVQEKSYEKWIREFRICDKIEYDKVIREETRPEKGKLRMGEVVR
                     IVSEITKGEAIVVTDVGQHQMSVARYYKFTEPNSLVTSGGMGTMGFGLPAAIGAKIGR
                     PEKQVVAFIGDGGFQMTIQELGTIMHFAIPVKLIILNNNFLGMVRQWQEMFFERRYAA
                     TELENPDFITIASGYGIPAKRVDKREDLESSIAEMLESDGPFLLEVTIDKEGNVLPMV
                     APGSAVSDITLTYKR"
     gene            18540..19100
                     /gene="ilvN"
                     /locus_tag="PK288_03125"
     CDS             18540..19100
                     /gene="ilvN"
                     /locus_tag="PK288_03125"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPM17941.1"
                     /translation="MKQEFTISLFTEDHIGIMSRITIILTRRQINIESITASESAVKG
                     VQMLTIVVRTTAEMVQKVAKQIQKLIDVLKVFVHTPDEIIYQELALYKVATKGLMSGN
                     MIEHIVRANHARILEISPEYVVIEKTGHKAEISELLHQLEPCGLLQFVRSGRVAITKQ
                     VKELNAYLAEMDEANKYTSEILESFN"
     gene            19207..20256
                     /gene="ilvC"
                     /locus_tag="PK288_03130"
     CDS             19207..20256
                     /gene="ilvC"
                     /locus_tag="PK288_03130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPM17942.1"
                     /translation="MAKIDFGGILEDVVTRDEFTIEKTREILKNEVVAVIGYGVQGPA
                     QALNMKDNGIDVIIGQAPEFKADWDKAVADGFVPGKTLFDIEEAASKGTIIQYLVSDA
                     AQRMLWPRIKPQLKEGDALYFSHGFSITYKEHTGVVPPSNIDVILCAPKGSGTSVRRN
                     FLAGAGINSSYSVFQDYTGKATDRILAIGIAIGSGFLFPTTFEKEVYSDLTGERGVLM
                     GALAGIMDAQYQVLRENGHSPSEAFNETVEELTQSLIRLVDEKGMDWMYANCSATAQR
                     GALDWRPRFKEAVMPVFKELYKRVKSGEECVRVLQSTGAPNYKELLNAELNELGNSEL
                     WKAGAAVRKLRPGNR"
     gene            20286..21779
                     /locus_tag="PK288_03135"
     CDS             20286..21779
                     /locus_tag="PK288_03135"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005826189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPM17943.1"
                     /translation="MEEKVIIFDTTLRDGEQVPGCQLNTIEKIEVAQALEALGVDVIE
                     AGFPISSPGDFNSVVEISKAISNPVICALTRAVKKDIEVAADALQYAKNKRIHTGIGV
                     SPVHIKYKIKTTPEEIIKRAVDAVKYAKKFVDDVEFYAEDAGRSENEYLARVVEAVIK
                     AGATVVNIPDTTGYCLPEEYGAKIRYLKENVPNIHKAIISTHCHNDLGMATANTMMGV
                     INGARQVEVTMNGIGERAGNTSLEEVAMIIKSHHSLNLKTNINSRLIFSTSRLVSNLM
                     SMPVQANKAIVGRNAFAHSSGIHQDGVLKYRENYEIIDPHEVGIKDSSIILTARSGRA
                     ALSHRLEMLGFAFGKDELDDIYHRFLLLADKKKEINDEDLRILSGEVFSGEKSLKLES
                     LQVICGKSALPVATVGIKIKGEIHVSTAAGNGPIDAAFTAVKQLISKTVHLEEFLIQA
                     ITKGSDDIGKVHIQIDHQGKIYYGFSANTDIVTASVEAFIDAISKIK"
     gene            21893..23296
                     /gene="leuC"
                     /locus_tag="PK288_03140"
     CDS             21893..23296
                     /gene="leuC"
                     /locus_tag="PK288_03140"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPM17944.1"
                     /translation="MEPKTLFDKIWDSHVVRSIKGGPDVLYIDRHYIHEVTSPQAFNG
                     LKKRGIPVFRPARTLATADHNIPTINQDQPIRDLLSRKQVEKLAENCTEHGIEFYGLN
                     HQRNGIVHVIGPELGFTRPGMTIVCGDSHTSTHGAFGTIAFGIGTSEVEMVLATQCIL
                     QLKPKRMRITINGTLQKGVTSKDLIMYIISQISTSGATGYFVEYAGQAIRKLSMEARM
                     TVCNMSIEMGARGGLIAPDEETLRYYKNSGAFPENFDWNQMTLQWKDLKSDPDAVFDR
                     ELEFNADLISPMITFGTNPGMGVAITESIPSIDKINGDASASFLKSLDYMGFKPGMKL
                     LGHPVDYVFLGSCTNGRIEDLRAFANIVRGRKKAPNVTVLIVPGSKMVERQAREEGLE
                     AILEDAGFRLREPGCSSCLAMNEDKIPPRKYAVSTSNRNFEGRQGPGARTLLASPLTA
                     AAAAITGKITDPREFID"
     gene            23320..23916
                     /gene="leuD"
                     /locus_tag="PK288_03145"
     CDS             23320..23916
                     /gene="leuD"
                     /locus_tag="PK288_03145"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPM17945.1"
                     /translation="MSKEKFEILESTCVPLPLQNIDTDQIVPARFLSATSREGFGENL
                     FRDWRFDKAGNLNPQFVLNNPEFGGKILVAGKNFGSGSSREHAVWAIYDYGFRAVVSS
                     FFADIFKNNALNNGLLPVVVPEEFLSELIDLVRKKPQTIVRIDLMNQVISIPSENRSA
                     GFDINPYRKDCLLRGLDDIDYLLSIKDEIIAFEEKNKK"
     gene            24033..25577
                     /locus_tag="PK288_03150"
     CDS             24033..25577
                     /locus_tag="PK288_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009293007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPM17946.1"
                     /translation="MKIEIMDTTLRDGEQTQGVSYSPLEKLQIASILLKDVKVDRIEV
                     ASARVSSGEFEALRKITEWARQNNIQRKVEVLGFVDGNVSLNWIHDAGGKVINLLCKG
                     SLRHLEKQLRKTPEQHVNDIREIIARAEKLDIQVNIYLEDWSNGMIHSEDYVYYMIDN
                     LKNETIARFMLPDTLGILNPELTYSFIGKMVERYPGQHFDFHSHNDYDLAVANVFSAV
                     KAGARGVHTTVNGLGERAGNAPLSSVIGVLHDQLNIETNINESEIIKISKIVETFSGV
                     RIPVNKPVVGENVFTQTSGVHADGDNKANLYFNNLLPERFGRKRKYALGKQSGKATII
                     KNLEEFGLNLSPESIAKITKRVIELGDKKENVTTEDLPFIISDVLGSDRSEFKIFIKN
                     YSLSLSYGLKPSASVLIDIDGKLYQETSPGDGQYNAFMKALRKIYDKLGKELPVLTDY
                     EVSIPPGGKTDALVETLISWNFRGREFRTRGLDADQTESAIKATEKMLNIVEGINGTE
                     KNHLSA"
     gene            25637..26698
                     /locus_tag="PK288_03155"
     CDS             25637..26698
                     /locus_tag="PK288_03155"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPM17947.1"
                     /translation="MARTYNIAVIPGDGTGPEVVREGKKALSAAALKFGFGINFTEFD
                     FGGDRYLKTGEILPDSAVAELSKFDSIFLGAIGHPDVKPGILEKGILLKLRFELDQYV
                     NLRPIKLYPGVETPLKNKGPEHIDYVVVRENTGDVYTGSGGVTMKGTPNEVAMQNMVY
                     TRFLVERCLRYAFEYTRKRNRKKTLALCGKTNVLTYVFDLWERAFNEIGSAEFPDIKR
                     EYYHIDATCMWMVKNPEWFDVIVTTNMFGDIITDLGAITQGGMGIAAGGNINPDGVSM
                     FEPIGGSAPKYTGKNVINPLASIACGGMMLEHLGEETAARAIEIAIMDVTANKLKDMA
                     AGKMGYSTSEVGDLVAERI"
     gene            27006..27863
                     /gene="hisG"
                     /locus_tag="PK288_03160"
     CDS             27006..27863
                     /gene="hisG"
                     /locus_tag="PK288_03160"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPM17948.1"
                     /translation="MKKLRIAIQKTGRLSENSQKLLEECGIKFSNGANVLKTSAVNFP
                     VEILFLRDDDIPQYVEQQVADIGILGENMVLEKDKDVEILEQLGFAHCRLSLAIPAQH
                     DFTGPEYLMNRKIATSYPKLLTDYLKENNITAEIEELSGSVEIAPGIGLADAVCDIVS
                     SGSTLMTNGLKEVVTILKSQAVLIANKNLDPERIGIVNKLLFRIRSVKNAKENKYILL
                     NAPEPAIAEICKILPGMKSPTVLPLAEKGWCSLHSVVKEDEFWERISQLREAGAEGIL
                     VIPIEKMIL"
     gene            27863..29155
                     /gene="hisD"
                     /locus_tag="PK288_03165"
     CDS             27863..29155
                     /gene="hisD"
                     /locus_tag="PK288_03165"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPM17949.1"
                     /translation="MRTIVNPPSSAWNDLCRRPVKDQSQLAVQVGKILSDVKNNGDRA
                     LLKYTLKFDGAATDDLRISEKEISEAEKEIPDELKSAIAVAGSNIERFHRTQLPDDGI
                     IVETLRGVRCWRKSVAVEKAGIYVPGGSAPLFSTVLMLAIPARIAGCREIIMCTPPDK
                     NGKVNQLILYTARISGVTSFFRVGGAQAIAAMAYGTETIPKADRIFGPGNQYVTKAKE
                     MVQLDGVPIDMPAGPSEVLVIADDSAEPSFVASDLIAQAEHGTDSQVLLVTTSTALLS
                     EVSKEIDKQLPALPRKAIAEKALANSLLISFDSIEKCMEFSNLYAPEHLIISVSDPAA
                     ATECVTNAGSVFIGKYSCESAGDYASGTNHTLPTNGFARNYSGVSVDSFMKKITFQEL
                     SGEGLLNLGPVIQKMAAAELLEGHRNAVTIRLNSLKNA"
     gene            29148..30179
                     /gene="hisC"
                     /locus_tag="PK288_03170"
     CDS             29148..30179
                     /gene="hisC"
                     /locus_tag="PK288_03170"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPM17950.1"
                     /translation="MLDINLLVRDNVRNLIPYSSARDEFRGRHGIFLDANENPYGKLN
                     RYPDPYQQELKLQISRIKQMPAENIFLGNGSDEIIDLAFRTFCRPGIDKVLTFPPTYG
                     MYEVSAGINDIPVIKVPLTDKFQINMEKTVPLLDDPHLKLIFICSPNNPTGNLLNSSD
                     IKYIIEHSRGIVLIDEAYNDFSGKQSFNTMIARYPNLIVMQTFSKASGLAAARIGMAF
                     TDQKTVQYFNKLKPPYNISSLNQKAAAGQMTHYEKTLDQVRKIISERDRLKTVLGSLK
                     IVEEIYPSDANFLLVRVKDPDYIYKSLAASNIIVRNRSRVVPGCLRITIGTKPENNRL
                     IAALKRISI"
     gene            30373..>31316
                     /gene="hisB"
                     /locus_tag="PK288_03175"
     CDS             30373..>31316
                     /gene="hisB"
                     /locus_tag="PK288_03175"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HPM17951.1"
                     /translation="MKKVLFIDRDGTIITEPLDEQVDTFEDLRFLPGVITALSKIAAE
                     TDFELVMVTNQDGLGTASFPEESFWPPHNKVIQILEGEGVRFAEVFIDRTFPEDNAPT
                     RKPGTAMLTKYLASGIDLESSYVIGDRVTDIQLAKNLGCKAIFIGDPNQPDAVLCTRD
                     WNDIYRFLKDIPRTAIIERKTNETSVSVYVNLDGTGKSSVSTGLGFLDHMLDQVARHG
                     TVDLTIRAAGDLHVDEHHTVEDVAIALGQCVSKALGRKKGIERYGFVIPMDDSLAEAA
                     IDFGGRPWLAWNVSFSREKTGDVPTEMFPHFFRSFCDSA"
BASE COUNT         9103 a         7248 c         6968 g         7997 t
ORIGIN      
        1 ccgttatcgt gcctgttgca gtggcaggct tacactgacc tgtggttgtt accgtataat
       61 taaacacccc ggaaacggtt ggcgttccgc taatggtaaa aactcccgcg ttgtatgatc
      121 ccgtgactcc tgcaggcagg ccggtaaccg ttgcaccggt tgcacttccg gaaacagaat
      181 agataatact ggtaatcggt gtgttaacgc atacggcctg aggtgtagga gttggcggtg
      241 aggttaaagt tataacctgg tcaggcaata ttcttattga tccgcttatc acgcctatac
      301 agcctcctaa ggtttgtaaa ttataagtat agtcacctgc tgtcgcaggt gtacctgtta
      361 ttgtatatgt cagcgcagtt ggattaaata aacctgtcac cccggggggg agattagttg
      421 gggttacacc tgttaccccg ggaataactc gataaactat tgctgcaatg gaggtattga
      481 tgcataccgt ctgattatct gttcctggcc cggaactcag tgcaatacat tgagacttaa
      541 tggtctcagc tgacaaagag acaagtatga ttaaaaataa tattcttttc attaggcttg
      601 atgtttttcc tgtttcaatc tgcagaaaat tttgtgcttt tacttaaatg gtttttgttg
      661 aacattaaag ggtaaaaggt cagcatcccc tatagttaac aaataatgat cctggtaagt
      721 tgtattttta atatctgatt taacatatgg ataacactga cttcagacac caatcataaa
      781 caaaggttat gccgaattaa atccaaactt tatgctaatt taactaataa tccacttatc
      841 cggctattta atttgaccaa ttagacgaaa tcgtacataa acgggaagta tgcaacaatt
      901 aaaaatccat agcccgaagc aggtcaggca gggttgaggg attatctgca gaggagataa
      961 cagttaactg ggaatcagct ttataatatt gaaatcctgc ctttatcaga tcaatatccc
     1021 gattgacttc ccccggatgt tccctttttc cgtttccatc aaccgggtca atcagtatga
     1081 gccttcgggg agccagggaa gcagccaggt cagggaggtc atattctttc agggcttccg
     1141 atactgtact gagaataaat agtggaagat aaaaccggca gtttgcgatg gaccggaaag
     1201 atgaataagg cccttcgaga ataatgcttc ttatttcatt gttgaaagct gcggcatgca
     1261 gaagtgccgg ggacattgct cccctggcaa aaccaatgat ttcagcttcc ggattatcct
     1321 tcagaatcag tctgaccagc ctggcaatat ctcctgcatg tattcccaat atgcttctcc
     1381 ctgtcaggat tgatgcatac caaaggttat gagaggcgcc gttaaaatcg gcatccccct
     1441 tgaaatcgcc cgatcccatt tctccaacac ccagcagatc aggagccaga atagtaattc
     1501 cttcctttac aatcatttca aggctcccgt cattaccgaa agcgtggttc tttccttccg
     1561 gatccaggaa taacattacc ctgttgccag gtactgaagg tttgaagatg agataaggaa
     1621 taatataccc ctccttaagt tcaataaaat atttttcaat cgaatagtca tcttttttta
     1681 tacgtccggt aaagacaggg tcaagtggct tctccggttt aatgtaacct gacaggcgct
     1741 cagctgatct gataacctcc ggataaaagc ttcccggatc ttttctcctg atatttagct
     1801 ccgcagtcaa tcgggataag cgcttgagat taagcgaata aaccgtttca cccgcaaaag
     1861 attcagacac ctggcctgcc gcagtgaccc gcagttcctc ctctgtaaga acttccgttt
     1921 caaaatcggc agggctgccg ggattattga gaaacttctg aaaaaaagcg tacattgctt
     1981 ctctgttctt tacagttgat tcatgcggcg cgtcatcctc gaccatgtta aaactttcgc
     2041 ctgctccata tgattcaaaa atcgcggaaa cttcattacg cgtctccctc gctccctgaa
     2101 tactgaaaat atccctggtg gttgttatca gcaaagtcgg ttttggagcc ctgataatca
     2161 gaaaatccgg atgatccaat ccgcttataa tcagtctagg gatattctgt tctgcatcct
     2221 gggggccgat cgactgaaaa agcctgctat agttggtaat gtagttttct ggtgctgcag
     2281 cgtaaatacg atcatcaaca gctgaaatat atgctgactg ggtccctcct cctgaacggc
     2341 cagtaatccc aatccgtgaa ggatcaacct cctttcttgt gagaaggtag tcaactgccc
     2401 ttattccatc ccagatcata tggaaagcct gcgaactgcc tgtcaagaac gcctgtgccc
     2461 cggggtatga atgctccttt gtaggccctc cgaccataga tttcccggtg gaaggatcat
     2521 aatattccag cctttcaccc tgcccgacag gatcaaaagc aaagacaata aatccttttt
     2581 taacaagatt ctgaataaca tggatatata caggacttcg ataaccctcg actgtatgac
     2641 cgctgcaata aataattgcc ggagctttct ttccttttga aacccccgca ggaataaaaa
     2701 gtgatgaagt tacataaaat cctggctgtg attcatatat aatatgttca atcctgtagc
     2761 ctgttttccc gatcaccctg gtcacccttg catttaaatc agttttggca ggaaatggcc
     2821 caatgagctc attcaatttc gtacggataa aattctgcct ttcctgccac tggtccaatg
     2881 ttctgatccc ggcaatgcgg gatgcacgtt cctcgagcag ctcgtaggcc ctttctgaca
     2941 ggtaatgata aagggaattc cttgcatcac tgaattccag ccaattattg ctcacaacat
     3001 tcagatcctc ctgtgccccg acgggtttac aaggcagcat tagtgcaatt accgtggcag
     3061 caaatacttt tacatccaaa cattgccaac ttccattttt cttccccgat tccataataa
     3121 tcagctattt tattaccaaa ccttcattgc ggcctcccgg tcagctgaaa tacatttgcc
     3181 tgacagaggc tgtaatatta tcagatttaa ttcttttccg ggacatgttt tagtattaaa
     3241 aagcaatccg ttgaggaaat attttcagat gaacaaacat ttgcctgacc taaaaataat
     3301 taatagttaa ttacgtttaa gaaatattga agatttcttg aaatgaaagt tacgtcaatg
     3361 gaaaaaatca aaatcaatat gctgaggttt gcgggtgttg ttgttttggg ttttgggctg
     3421 cggtcatttg ttgtatgtca ggcacctgta caagacatac tccccaagag taccctgagg
     3481 gatcagatga attatgtcca ggagaagacc aggatttatg acaattaccg ggctataagg
     3541 gaagatgttt ttcagcagat aaagagaaat tcacttgatt cactgcaggt atcgaaaaca
     3601 gagattaccc gtttaaaagt gcttgctgag tcaaagaaca gagagattga ctccctgaat
     3661 tctaccctgt cagccaggga aaaggaactg caggagatga caaccttcag gaacagtatc
     3721 cggttatttg gtattgaggt ggacaaacgg acctacaagg ggataatgtg gctggtgatc
     3781 gcaatactgt caatcatact tatcagtggc ttcctctcat ataaaaggaa tcgtattgta
     3841 acgcacaata ccaggaagga attcgaggat cttaaaaaag aatttgaagc ttaccgcaaa
     3901 gcctcgcgtg aagctcgtga aaagatgtcg atggcgcatt tcaacgaact gaagaaactg
     3961 cgaaccggct gacagcagat tatcacattt ttatcgctta tcggccatcc cctttttcct
     4021 gccggtattc tgcagcaaat tcgtcagcta ttgaattgat aatatcatcc gtcaggtgca
     4081 ttccggaatt aataaccatc ctgtacctga aaatcacttc ccctcctgca ggaaccgaga
     4141 aatcgaccct ttcatttccc tgagtaaaat caacagcacc aagcggattg gctgaaaaca
     4201 atccataacc gcgtgcatgc cagtaagtgg gataagatac attatccgga tgatcaatca
     4261 tcactattga aatctcatca ttgtttataa gtcctgtcaa attcatccat ctggccctgg
     4321 ttccccaaac cgcatctccc tccttccctt cgctactcct gtaattaccg gttactccaa
     4381 tattttcacc ggctttctcg gctcctgggc tgccagactg atccagcagc aaatcgtcat
     4441 ccgttgaagg catttccagc tggcgggcaa cccgtatcgc atacatgcct tccttggtat
     4501 ccttcatgct gacagggata tcatttgctt tcagaacggt tatccggtcg attatatagt
     4561 atttaccccg ggcgaaaaag ttaaattccg ttctttcgga taataattcc tttcctgacg
     4621 gatcctgcca gcttgccgaa atattcatcg tccctttgcc atttcctccc tccacattat
     4681 caattttcag cagcctgatt tctccaccgc tttcactttt atgtccttct gacccattac
     4741 cccagaagtc aactccgtta acatcaccgt agttgaacca tatacccacc tggtgaatat
     4801 ggtcattacg ctctccggca cgcggcctca aagggaaacc gcgggtaacc tccgtgccgc
     4861 tgattgtata aaccggatag aaaatcggct tataaacatt gaccggccag caaaaagatg
     4921 taacaagctt tcccccgata agaacatcaa ctttacgttc tgcctccttt tttataaaat
     4981 caacagcctg atttttatct cctccggctg atgagcagga aatcaccaaa aaagcggcaa
     5041 gaaaaaggac ggtaaaggtt ctcaacaaat ctgatacttt catacacgat taatatttta
     5101 aaatttacta ctgatgattt aaacgattct ctatttctga acctttcact ggatacctgc
     5161 atgcattgcc cgggtacatc aaaccaattt cattgcagat tacatatctg accggaatgc
     5221 aattacccga ctcaacttcc ctggattctg atctttccgg cgtcatccct tgctttctga
     5281 agtatcgtgc tgatctcaac gggccggccg ccgtttttcc tgctttcatc agctgcagcc
     5341 ataaaggcaa atatctcaat tgtttcttcc tgactcacgg gcggaatccc ggtatcgaaa
     5401 aatttcacaa cttgttcaag caggggatta tatcccgaat attttccaag atccacgacg
     5461 gctttttcac aaaaggccgt tcccccgtaa tcacccctgc ctgccctgat ccccctgaag
     5521 gtcccaatcc gttcattgtt ccataatccg gtcaccaaat ctgttccttc agtaaatatc
     5581 cttgtaacag atttgcatcc tgttcccatc actgtaaaca gtgcctccac cccgtgaatt
     5641 ccataccaga acagatcggg gtgggttttt tcgattgtag ccggactgta agtgtcggca
     5701 cctatcactt ttccagcaga gccattttta atttctttta taccaggcag atatctcagg
     5761 gaggatgaag aaaaaaccgg caccctataa taccctgagg cattaaaaat tgcaattgcg
     5821 tctggcaatg aggcagctat cggcttatcg ataaacatcc tttttccagc cctgaaaacc
     5881 ggcaaagcct gttcaaggtg gatcctgccg tcatttgttt ccagtaaaac ggcatcaacc
     5941 tgtcttaata attcgtcgat cgatcctgta atttcgacac caagtttttt cacgtcttcc
     6001 gtatatcccg ggatcctttc aacgctcgat ttgatatcat tgcttcccct tggataggcg
     6061 gccacaacct tccagcctcc gaattccggc cccgctgaag gttcgttaag agcttgtgta
     6121 aaagcaacac tgtgtgatgt gtcaagacct attataccga tcctctttcc ggaagaacct
     6181 ttaagatgtc ctgctgaaaa accatttccc gtcatggtca gtccgacact tcctgctgca
     6241 accgacctga taaaattcct gcgttccatt aaatctcata ttaaacaaaa ttcatatcct
     6301 gatattttcc ttagcaatca tatcacatcg ctgacgacat atggtttcct gtaatcccgg
     6361 gtcagcagta ggtcggcctc cctgtcattg acaaatttct ccctttgccc gtcaaatgtg
     6421 agttttctgc caagcctgta ggatatgttt gcaaggcttg gaagggttga tgataaaaat
     6481 ccctccacaa tgggacaatt aagatcctga tccctgccgg aacggatcgc gtcaataaaa
     6541 ttggcataat gttcagtacc gcctggtgcc gccagatagc cggtttccgt taccctgggt
     6601 tcatctttgg cagaaccggc aaaaggttcc gtttcacgtt tcctgaatgc tttccacgta
     6661 ctcccgtcaa gctcaaggta accttccgta ccgtaaaaaa tatttcctat tctgattcca
     6721 aggctggctt cgtcattcgt ataacgaccc ctggtttcaa actcaagaat tttcccatcg
     6781 ctgtaactga aaagcgatgt ctgcgtattc ggagtctcct gagcgcactc cgaaggatcg
     6841 ataccgaaca gtcctcccac ggaaaatact gaaacgggat gctcattttt attaagaccc
     6901 cacctcgcaa tatcgaactg gtgagggccc tggtttccgg tatcaccgtt tccggtatcc
     6961 cagaaccaat gccaattgta atgaacccgt ttttcattat acggccggta aggagccggt
     7021 cccaaccaca tgtcataatg cagtgaagca ggggcagcgc tgtcaggggc aatgccgaac
     7081 gaatccctgg gtttgtagca aagtcctttg gccatgaaaa catcacctat tcctccttca
     7141 tgaagaaacc tgatagcatt cacaacattt ttgatagaac ggttctggaa acctgtctga
     7201 accctaacat tgtacttgcg ggcagcttcc accattttac gtccttcaaa aatattatgg
     7261 ctggccggct tctccacata aacatgcttc ccggcctgac aggcccagat tgttccaagg
     7321 gcatgccaat gattgggagt agcaaacgaa acagcatcaa tatccctgtc gtcgaagatt
     7381 ttcctcatgt cccacggggt aacgactttt tctccggttc gttcgagaat tgtttttgca
     7441 gccgcttcaa aatattgttc atcaacatca cagatggctt tcagccttac attacggtta
     7501 tccttaaggg tacaccagtt gttgatatgg cctccgccct gtcccctgat cccgataaca
     7561 gcaatattga ttctttcatt tgatccgatt atcgaactat aggctttagt gccaaacccc
     7621 atgccgccta ttaccatacc ggctgaacct attgcgcttt tcctgataaa atctcttctt
     7681 tttgtcatta tcattattta ttaattatac aagcaaccag taaactaaac tgaatatcct
     7741 gccagcctgt taaagatctt cagctgctcc ttcatagccc caaccctgtc agcgggttca
     7801 gtacgtgcct ccagcaggat ccactcctcg tacctgatac ccgacaaaag atcaaaaagc
     7861 tgctggtaag gataatccgt tgaatcgagc tccctgacat ggatagtgtc accaaaccat
     7921 ttcttcacca tgttgaagtt cccttcaagt ccagggggta aaagatcctg gtcattgcag
     7981 ttccagcata tttttacgtt atcctccgta acctggtcga aaattgcctt catcaccggc
     8041 agttcctgtg taatttcacc atgaacctca actcttataa gctgatcaaa atcacgagca
     8101 aaccggccta cttcattaag agatccggca atctgggcta tcgttttttc acggggaatc
     8161 ccgggtggaa gataattggg ctttaccttt attcccgttg cacctatgtc tctgcatagt
     8221 ttaatatatt ctttcgtctg ttcaatgttt tccctcaact gatccggatc aggactgtga
     8281 tactcaaaat ttgaaccgta gccaacgcat ttaacagggc tgtcttcaaa cattttcctg
     8341 acatccagcc gacgggatga gctaagactg gtttcgaccc cgtgagcatg ctgtgtcctg
     8401 agctcaacac cttcataacc cgcttctgcg caatttgcta tcagggtcgg aagatcccag
     8461 tcccttcccc attgatatgt caccagccca aatttcattt taccggggtt tctgcctgaa
     8521 catccctgca ctgaacccaa gactgccaat cctgctcctg aaataacact gttccttacg
     8581 aaacttcgtc tagtcattcg atttttcatt cgtatatttt ttatggtcga tgcctgcatg
     8641 ctcctccgag gcagggaacc acatatgagt gaaagcgaac attgtcaatc acgcttgtgt
     8701 tcctcgtcag tcatgcggga ttcatcattt catcctttta tgaaacatat ttaaattatc
     8761 ggatcccctg atctcatttc ccggtaatac ccctccggca tataccggcc cggatttatt
     8821 caacaggtta ctggttaaaa accactgatt aaaacggcaa tcaggcccgg tgctgctgct
     8881 ttgttttttt ctctttattt catcctgagc cagtgtctgt atacagttgc cggaataaaa
     8941 gcacctggtt aaaatgctca cctgggcata accacgtaat tggctgcatt taaaaattca
     9001 aggctttttg cacaacttgt aaactggtca aaattatatt cttccacctc aactattccg
     9061 tacttgagcc ccgatttttc agctgcggcc catattgcct gaaaatccat cattccgctt
     9121 gctcccacct cttccttatc cttgatatgc cataattcaa aacgtccggg atatttgttg
     9181 aaataatcaa ccggattttt acctcctacc acgcaccagt aaaggtcgag ctggaaaata
     9241 accttggacg gatcggtgtt ttcaagcatg aaatcatata ccgtggtgcc atcgagctgt
     9301 gttgaaaact ccgcatcatg attatgatac ccgaaccgga tcccctttgc ccggcatttt
     9361 tctccgatca gattgaaata atcgcaatac attttcagtg tgtcaagcga cctgtaggcg
     9421 ctggcaccca taaagggctg tacaaggtag ctgacacctg cggcagcatg tgcatcaatg
     9481 caggcatccc accaggccat ggttgtttcg cggctggctt catccggcag tacctgaccg
     9541 gtatgggagc ttatcaggcc caggccgtta gttgcacata aattcctgaa tgccactggt
     9601 tccataccgt aaaactgtcc gtccctgtaa cctgcaggct ccacgaaagt gtaacccatt
     9661 gctgctactt ttgcaatggc cccgggaaca tccctgcgta ttgaatcgcg tatagaatat
     9721 aactgtaatc cgatattctt tttaggagct tccctgcagg aattaaacgg cagggaaaca
     9781 agaccgaaaa aggcaaggac aacaagaagt gaaaacaggc ttggtttttt cattttttcc
     9841 ggtttttaag ttgaaattat aaaataatca ggtctgcctt tccataatga gttctaaaga
     9901 tacaacttat cagtaacata taatcattcg cttatttgat tcatataatt tttcagatcc
     9961 ctgattatgg cagaatcaga ttccggattg aatctgagta aattaaccaa tgtattctca
    10021 aagtctgact gtaaggcatc aataagctgg agatcgcttt caaaattgct tccgctttcg
    10081 tccgtggctt taaaaacaaa accatacgac attgccttca gtatcttctg gtatggttta
    10141 ccatgtctga ctgcaagcat tacggctccc atgaaccggt caccagagcc gagtttcctg
    10201 cccagatcat gacctactct gaaaacggtg tctctgagaa acctgttccg gaatctcgac
    10261 agcaaatctt caatgtgctc cgcaagatct gttgccgtaa attctccggg gtatgcttcg
    10321 ataaggattt ctgccgcctg catcatgact tcgcgtgtaa accctgacac ctttgcgtca
    10381 ttaagcactt cgtacatgta agctgattca gggtgcaggt aataaccata atatgccgca
    10441 gtggcatgtc caagattatg gatgaaagct tttctgtcga cccatgcctt aatattttcc
    10501 tttggggcga gtcctttaat ttgggggatg ggagtcctaa accctttttt atcgagaata
    10561 aggatgttgt agggctcggc aaaaacctgg agaggatctt tttcaagatc cgactggctc
    10621 atgatcggaa ccattttgcc aatgcttgtt tcaacaaggc ctgtcatttc atttaatgga
    10681 aattccggag gcagaaactt cttcaggttc tcctccataa aatcccttcc agaaatcata
    10741 ttttcagcga taattatgtc gaggggctgg ccgggaaacc tgtcatgcct cagcttcaga
    10801 ccatcagcaa tcatgggaac aactttttca acggcattct ttcccacgct tacggcgacg
    10861 atgccggcag tggaaactgc ttcccctacg gccgggacat cagcggcatc aattgcgctg
    10921 acatttctca caactattgt ttcttccctt tccgatttgg caaccaccct gtaacatttc
    10981 ctttcattga taagggctgt cagcagccgg tcagtatcga caaaaactac cttgtacccg
    11041 gccgttccga atagctgccc tatgaaggaa cgaccaattt tccccgcgcc gaaaattact
    11101 attttccgat tgtcaacctg aaatctgctc tttgtaggaa tcatagtagg gtttcattct
    11161 ggaaagtttt tctccttcgt attcttcaaa gtaggtccct ccaatataaa aacaggcaaa
    11221 tccccctgaa acaattcccc agcagcaggc ttcaacaaga tctggttgtg gcgtgcccct
    11281 gatcatctgg ttaaccaggg atgcaataac ccctcctgca aaattatccc cgcaacctgt
    11341 tgtatctcct ccgccggact gtttaagctc gtcaatcaca cgggcagagg ccggaagctc
    11401 cattctccgc gtgtgggcaa ataatctgcc gtcggagagt aatgtaatat tcctgggccc
    11461 attagttaca atgatcgatg atactttcag gctttcaaaa tacgtcactg catcatcgat
    11521 gctgttttca ccgctcagcc gcagagcttc ttccctgtcc attatcagga gatcaatgta
    11581 cccgtagctc ctgtcatctt tccccaatgg ccatttccgt gaaggatcag ccttctcgct
    11641 cctgaagtcg aaaacagtat tcaccactgt gatacagcca ttgttcttag ccttttccag
    11701 aagaacggaa agatcatcat gaagacgggg aacgagtgcc gttcctccga atacaacaat
    11761 gtgtgagctg aaaaaatcat catccagatt gtcccggcca aactcccagg ctgagcctat
    11821 tgaatttata aatatcctct cgccgtgccc gccttcaaaa agaggatcag acaatactat
    11881 cgttgaaggc gtgctgcgat caatcagtct gtaattacga agcttaatgc ctgtcctgcc
    11941 aagggaggag atcagaaagc ggcccatgtc atcatttcct ccgagcccat aatatctcac
    12001 ttcacattga tgtttatcaa ctatttgtgt gacatttatc aacgatacaa ttgaaggacc
    12061 cccgatactg attttatcat gtttcctccc tcccgtaatc tttccgacag cgtttccaac
    12121 aggaatgccg gtaaatttct caaactcttc ggtaaaaacc aggtttcccg gcacaagtcc
    12181 gccatctcct cgcttttcag acaagaaagg ggcaattgac tggccggtga aatcaatatc
    12241 attataaagc aaatcaacca ggcagcaacc cgcacctgag actatgattt ttttcggcat
    12301 aatatgttac agtttagaaa gacagaccag aaaccggata atccgtagca tctgaaatat
    12361 cctttcccag aggattacag gctcaggcat catgattcag catgtgaaat tgccggaagc
    12421 gaaaaatgaa aagggtcaga ggaatttttc atagtcattc acaagcagat gtgaaggcat
    12481 cacatcttca attatttcag ggaatcttcc cgtttcctca taaaaatggt tatcaccggc
    12541 atcatcattc acaaggctga cctcacctat cagcacactg cccttacctt tctggccata
    12601 aaacctgtga taaacaccgg gcttcaggca tatgctctgg cccggcctga ggataagggc
    12661 cctgccggca tttaccgtgg taagtactcc gtcgatactg accgtcaacg gtgcgtctga
    12721 aagcttacta ccggaagttg attcatacag ttccattacc aggttacccc cgcccctgtt
    12781 aattatatct tccgatttat tccggtgaaa atgcatcggt gtttcctgct cctccctggc
    12841 catcattatc ttttcacagt acagtttatc ctttttatcc cagcttccgt tccttatcgt
    12901 gaaaaggcaa aggcctgtcc tgaaaaagtc gccgctgcca aaatcagtaa tatcccagcc
    12961 cagcttattg tcaacgatct ctgaacagat attatatttc cccttccagt cggcgggacc
    13021 ccaggatgcc cattgcggaa gaaaaaacct gttgtccgca agaaagccgg caaaagattc
    13081 aattatactg ttaatctcac ttcttttcat tatcagatca gtatttttta ttatacccta
    13141 ttccaaatat aaatattttc cctgtgatgt gcagcagaga aaaatcaaaa cctctgacaa
    13201 atatcatctt cacgaatggc aatggtaaaa aagggtgccg accgtaagga ccttcccgga
    13261 aaccggcacc cggataataa aaatcatctt tattattgga tttaataata ttttttctac
    13321 atttgtatgt tttttgtata ttattgtaat tattttattt attttgcttc aaaatttaaa
    13381 ttcaactgat aataaataaa accatgatat ttcatgcagc caccgcgcaa accgcaacaa
    13441 gaactgatgt tcttctcaca gaggagatga ttgatttcgt caaatctgat ttcgggaaca
    13501 acctggcacg caatctgaat ctggtgaagg tatcctcacc gctggtcgtg ccggacggta
    13561 cgggaattaa tgatgatctc aacggaatag aaaaacctgt aagattcccg gttaagtcgc
    13621 tggataacac aaatgccgtt attatccagt cacttgcaaa atggaaaagg atcaggctgg
    13681 agtattttaa tatcggggcc ggcaggggga tccttacgga catgcgcgcc ctgaggccgg
    13741 atgagaaact aagtcccata cattccctgt atgttgatca gtgggactgg gagaagcaca
    13801 ttcgtccgga tcagcgaacc ctgaattatc tgaagtccac tattgaagat atttataaaa
    13861 cattcaggga aacggaagag aagttgtacc ggaaatatga atggctggtt ccccagcttc
    13921 ccgaaaagat cacttttatc cattccgagg aactgttgca gcaatatccc tatctgaatc
    13981 caaagcagcg cgaaaatgcc atagcccgtg ttcatggcgc tgtatttatt ataggtatag
    14041 gcagcaaact cagcaacggt gagccgcacg acggacgggc tcctgactac gacgactgga
    14101 gctcagtaaa tgaagacggt tattatggcc tgaacggcga tattattttc tggaaccaaa
    14161 cgcttgattc ggctttcgaa atatcttcaa tgggaatccg tgtcgacaaa agctcgatga
    14221 aacgccagtt acggttatgc ggctgccagg aaagggagaa actgatgttt cacagcatgc
    14281 tcctgaatga tcagcttccc ctcagcattg gcggaggcat aggacaatcg aggctttgta
    14341 tgtttttcct caggaaatca cacatcgggg aagttcagtc gagcatttgg cccgacgata
    14401 tcatgacagg tgcgatggaa aacggtttaa agcttctctg aatacttagg atcgtcacgg
    14461 ggcaacggct ggttggtgcc attaaccggt agaagcaacc tttttcagcc gcggattcat
    14521 tccctgctca ttgtgttatt atttattttt cccaaaaaac aaaaaaaaga gaaattttat
    14581 ttgatttatt aaaataatat atacttttgt cgttcaaatt atctcaaaaa gtgcataaag
    14641 ctattatata tattattgtc ctcgtactta ttctcacagg aagtgaatga tccgggaata
    14701 atatgtatag gagattaatg tacagaagct ttccggatca accggaaagc ttttttttaa
    14761 catatttctg aagcatgaaa acaaaaatca atttgagaag ctcaaagaca accaccggaa
    14821 gaaacatggc cggggcgcgg gctctgtggc gtgccaacgg catgaaggag gaccagttcg
    14881 ggaaaccggt gattgcgatt gtcaattcct ttactcagtt tgttcccggg catgtgcatc
    14941 ttcacgacat tggccagcat cttaaacaac agattgagaa atccggattt tttgctgctg
    15001 aattcaatac aattgccatt gatgacggta ttgccatggg ccatgagggc atgctttact
    15061 ccctgccctc aagggagata atagccgaca gcgtggaata catgtgcaat gcacaccagg
    15121 ctgatgcaat gatctgcatc agtaactgcg acaagattac cccggggatg cttctcgcaa
    15181 caatgaggct gaatattcca actgtgtttg tttcgggagg accgatggaa gcaggctata
    15241 acaagggaag attcctcgac cttattgacg caatggtagc tgctgccgat aattcagtca
    15301 cggatgatga gatacgggag attgaaatgt ctgcatgccc cacatgcgga tcatgttcag
    15361 gaatgttcac agccaattca atgaattgcc tcgttgaggc tatcgggctt gggctgccgg
    15421 gcaacggtac aattattgcc actcatacga accgcctccg gctttttgaa aaagccgcat
    15481 ccctgctggt tgatatagta aagaaatact attatgaggg cgatacctca gtgctcccga
    15541 gatcaatcgc aacaaggcag gcatttatga atgccatggc tctcgatatc gccatgggcg
    15601 gatcaacaaa taccgtactg caccttctgg ccatggcaaa tgaggctggt gtggatttca
    15661 gcatgaaaga catagatgaa ctttccagga aaatacccaa tatctgcaag gttgcgccca
    15721 gttcctcata tcatgttcag gatgttaacc gggcaggcgg cattctgtcc atcatgggag
    15781 aacttgaccg gaacggcctt attgatccgg atgtcaggcg cattgattac ccgactctcc
    15841 gcaatgcctt gcgcgactgg gatataatga gtgagaaacc cctggacgaa gccctcgatg
    15901 tgtaccacag tgccccttcc atgagaagga acctggtact ggcttccaac gacgcaaggt
    15961 atgaagccct tgacaccgac agagaaaaag gatgcataag gaatattgaa aacgcctata
    16021 acaaggaggg tggacttgct gtgctttacg gaaatattgc accaaaggga tctattctga
    16081 aaacggcagg cattgaccgg tcatgctttt acttcagggg gccggtcgtg ctgttcgaat
    16141 cgcaggaaga tgccgttgac ggcattctgg gcggcaaagt gaaagaagga gatgctgtgg
    16201 ttatcaggta tgaagggccg aagggaggac cgggaatgca ggaaatgctt tatcccacat
    16261 cctatttgaa atccaggctg cttgacaaga aatgtgccct gattaccgac ggccgctttt
    16321 caggcggcac gtcaggcctt tcgatcggac atgtatcacc cgaagcagca gcaggcggcg
    16381 caatggccct cctcagggat ggagatatga tagaaattga tgttaacaac cggaaaatga
    16441 atgttttgct ttcagacgac gagctggcag caaggcgcag gacggaagaa tcaaaagggg
    16501 aaaaggcttt caggccggca accaggaaca gaaccatttc cgcatcgctg agggtgtatg
    16561 cctcaatggt cagctcagcc gatctcggag ctgtcagaat aatttagagt gcattaaacc
    16621 atacaaattg caaagggcaa agattggaaa aaattaacaa tattattgtt ttggaatcta
    16681 tgaaaacaga aaaagaaccc attacggtta ttgaagcaaa gaacggaaat ggaacgatat
    16741 caggggccga agccctgctg aaatgcctga tcgaagaagg cgttgacacc atcttcggtt
    16801 atccgggagg tgccataatg cctgtatatg acaaaatgct cgacttcagc agtaatataa
    16861 ggcattacct ctcaagacat gagcagggag cagcacacgc ggcacaggct tacgcaatgg
    16921 ttaccggaaa gccgggcgta tgtttttcaa catcaggacc cggagcaaca aaccttgtaa
    16981 ccggtattgc aaatgccttc ctcgattcca tcccggttgt tttcattact gcccaggttg
    17041 catctaacct gataggcacc gatgcattcc aggaaaccga tattcttggc atctcaatgc
    17101 ctgtcgtgaa atggaatgca caggttaaaa aagcccagga tattcctgaa accattgcca
    17161 aagcttttta tattgcaaaa accgggcgac cgggccccgt attgattgat ataaccaaag
    17221 acgcccaggt tgacactctg aactttacat ataagaaatg caactacatc agaagttaca
    17281 atcctgttat cgcactgaat accagatctg tggaatctgc tgcaataatg ataaaccatg
    17341 cagaaaaacc gcttatcctg gccggacatg gtatcctgat ttcgggagct gaaaaggaac
    17401 ttctgcagtt cgcagaaaaa accggaatac cagttgccgt aaccctcctc ggcctctctg
    17461 ccttcccgtc ggggcaccgg ctgtttgcgg gattccttgg aatgcacggc aattacggac
    17521 caaacctgct gacaaatgaa gctgatctta ttattgcaat aggaatgcgg tttgatgacc
    17581 gtgttacagg aaatattaaa aagtatgcaa aaaacgcccg gattatccat attgagattg
    17641 acgaggctga gatcaacaag aatgttcttg ttgatgtcgg aattaacagt gatgccaggg
    17701 tggcactgaa gcatctcact ccgctggtgc aggaaaaatc atacgaaaaa tggatacgtg
    17761 aattcaggat atgtgacaaa attgaatacg acaaggtaat cagggaagag accaggcctg
    17821 aaaaaggaaa gcttcgcatg ggtgaggttg tgcgtatcgt atctgaaatt acaaagggag
    17881 aagcaattgt tgttacggat gtcggccagc atcagatgtc cgtggcaaga tattacaaat
    17941 ttaccgaacc aaactctctt gtcacttccg gtggaatggg cacaatggga ttcgggctgc
    18001 cagcagcaat tggagccaag ataggaagac cagagaaaca ggttgttgcc tttatcggtg
    18061 acggcggctt ccagatgaca atccaggagc ttggtaccat tatgcatttt gcgatacccg
    18121 taaaactgat cattctcaac aataatttcc ttggaatggt ccgtcagtgg caggaaatgt
    18181 tcttcgagag aaggtatgca gcaacggaac tggaaaatcc cgactttatc acaatagcca
    18241 gcggatacgg gataccggca aaaagggttg ataaaagaga ggacctggaa agctcaattg
    18301 ccgaaatgct tgagtcagat ggaccgttcc tgctcgaggt taccattgat aaggaaggaa
    18361 atgttctgcc aatggttgca cccggatcag ccgtctctga cattaccctg acatataagc
    18421 ggtgaaccac caggtaagac ctgagagccg aagggaacgg cccgggtaaa acatatccgg
    18481 gcagcaatat gaacaaaaag tataaaaacg aacacgacat ctgagaaaaa caaatattca
    18541 tgaaacaaga atttacaatt tccctgttca ctgaagatca cattggaata atgagccgga
    18601 ttacaattat ccttacccgc cgccagataa acatcgaaag cataactgct tcggaatccg
    18661 ccgtcaaggg agttcagatg cttaccattg tggtcaggac cactgctgaa atggtccaga
    18721 aagtggcaaa acagatacag aagctcatcg atgttttaaa ggtattcgtc catactccgg
    18781 atgaaatcat ctatcaggag ctggcacttt acaaggttgc gacaaaaggt ctcatgtcag
    18841 gtaatatgat cgagcatatt gtccgggcca accatgccag gattcttgaa atttcacccg
    18901 aatacgttgt aattgaaaaa acagggcaca aagccgagat aagcgaactc ctgcatcagc
    18961 ttgagccatg cggcctgctt cagtttgtaa gatcaggaag agtggccatc accaagcagg
    19021 tcaaggaact caacgcttac ctggcagaaa tggatgaagc caataaatat acatcggaga
    19081 tcctggaatc ttttaattga accaacaggc aaccaggcca ttcacaggga tcaatgcccg
    19141 gggccgtctg cctgagaaaa atcatgaaac cagttaataa cagacttaaa aaccaatctt
    19201 aaaatcatgg caaaaataga ttttggcggc atcctggaag atgttgtgac cagagatgaa
    19261 tttacaattg agaagacacg cgaaattttg aaaaacgaag tagttgctgt aatcggatac
    19321 ggggttcaag gtcccgccca ggcactgaac atgaaagaca acggaataga tgtcataata
    19381 gggcaggctc ctgaattcaa ggctgactgg gacaaggcag tggcagatgg ttttgtgccc
    19441 ggcaagacat tgtttgatat tgaagaagct gcatcaaagg gcacaattat tcagtacctg
    19501 gtttcagatg cagcacagcg catgctctgg cccagaataa aacctcagct caaagaaggg
    19561 gacgccttgt atttttctca tggattttca ataacctaca aggaacatac cggagtggta
    19621 ccgccttcga acattgacgt gatcctgtgt gccccgaagg gctccggaac aagtgtcaga
    19681 aggaactttc ttgccggcgc aggcatcaat tcaagctatt cggtatttca ggactatacc
    19741 gggaaagcca ctgaccggat actggctatt ggaatagcaa tcgggtcagg atttcttttt
    19801 ccgacaacat ttgaaaagga agtttacagt gatctgactg gtgaacgggg ggttctgatg
    19861 ggagccctgg ccggcataat ggatgcacaa taccaggtgc tgcgcgaaaa tggccattcc
    19921 ccctctgaag cattcaatga gacagttgaa gagctcaccc agagccttat caggcttgta
    19981 gatgaaaaag gcatggactg gatgtatgcc aactgcagcg ccaccgcaca gcgcggtgcg
    20041 cttgactggc gtccaagatt caaggaggct gtaatgccgg tgttcaagga actttacaaa
    20101 agggtaaagt caggggagga atgtgtaagg gtactgcagt ccacaggcgc tccgaattac
    20161 aaggaactcc ttaatgcaga gctcaatgaa cttggaaatt cagagctctg gaaagccggt
    20221 gcagcagtaa ggaaactccg cccgggaaac aggtaacagt taacagaaaa atgatagaag
    20281 acaaaatgga agaaaaagtc attattttcg acacaactct cagagatgga gaacaggtcc
    20341 cgggatgcca gctgaataca atcgaaaaaa tcgaagtggc ccaggcgctt gaagcactgg
    20401 gagttgatgt aatcgaggca ggcttcccca tttccagccc cggcgatttc aacagcgtcg
    20461 ttgaaatatc aaaggctatt tccaatccgg tgatctgcgc acttaccagg gctgtgaaaa
    20521 aagatattga agttgctgcc gatgctcttc agtacgcaaa gaataaaagg atccatactg
    20581 gaataggcgt ttcacctgta catatcaagt ataaaatcaa aaccactccc gaagaaatta
    20641 tcaaaagggc tgttgatgca gtaaaatatg caaaaaagtt tgtggacgac gttgaatttt
    20701 atgccgaaga cgccggaaga agtgaaaatg aatatcttgc aagggtcgtt gaagcggtta
    20761 tcaaagccgg agcaaccgtg gtaaacatac ctgacacaac aggatactgt ttacccgagg
    20821 aatatggcgc aaagatcaga tacctgaaag aaaatgtacc gaacatccac aaggcaatca
    20881 tctcaactca ttgccataat gatcttggaa tggcgacagc caacacaatg atgggtgtaa
    20941 ttaacggggc caggcaggtt gaggtgacaa tgaacggaat cggggaacgg gccggaaaca
    21001 cttcacttga agaagtggca atgatcataa aaagtcatca tagcctgaat ctgaaaacca
    21061 atatcaactc caggctcatt ttcagtacaa gcaggcttgt atcgaacctc atgagcatgc
    21121 ccgttcaggc caacaaagcc attgtgggaa gaaatgcctt tgcccattca tcgggcatcc
    21181 accaggatgg agtcctgaaa tacagggaaa attatgagat aattgatccc catgaagtgg
    21241 gaataaaaga ttcttcaatc atcctgactg cccgcagcgg aagggcggca ctgagccacc
    21301 ggcttgaaat gcttggtttc gctttcggga aagatgaact ggacgatatc tatcacaggt
    21361 tcctgctgct tgcagataag aagaaggaaa taaatgacga ggacctgagg atcctttcag
    21421 gtgaagtatt ttccggggaa aaatcactca aacttgagtc tttgcaggta atttgcggta
    21481 aatcggccct gcccgttgcc accgtgggga ttaaaataaa gggtgagatt catgtctcaa
    21541 ctgctgccgg caacggtccc atcgatgctg cattcactgc agtaaaacag ctgataagca
    21601 aaaccgtcca tctcgaggag tttctgatcc aggcaatcac caaaggcagt gacgatatcg
    21661 gcaaggtcca tatccagatt gaccaccagg gcaaaattta ttatggattt tcagcaaata
    21721 ccgatatagt cacagcttcg gttgaagcat tcatcgatgc aatctcaaag ataaaatagg
    21781 tgaatccctg tcataaaatg attcagccag gacgttttgc atacttaatt atcaggagaa
    21841 taaaaacgtt gaagaacagc tttttatgtg ttaaatattt cacaggtact taatggaacc
    21901 aaaaacactt tttgacaaaa tatgggactc tcatgtcgta cgttccataa agggaggtcc
    21961 cgacgtactg tatatcgaca ggcattacat acatgaggtt accagtccgc aggcattcaa
    22021 tggtctgaaa aaaagaggga ttccggtatt ccggcccgcg aggaccctgg cgactgccga
    22081 tcacaacata ccgacaataa atcaggatca gccgattagg gatctgcttt cgagaaagca
    22141 ggtggaaaag cttgccgaaa actgtacaga acatggtata gaattttacg gactcaatca
    22201 tcaacggaac ggtatcgtgc acgtcatcgg gccggagctt ggatttacaa ggccgggaat
    22261 gacaattgtt tgcggcgaca gtcatacatc cacccacggg gcattcggta caatagcttt
    22321 cggcatcggt acaagcgagg ttgaaatggt tcttgcaacc caatgcattc ttcagcttaa
    22381 accgaaaagg atgcgcatca caatcaacgg tacattgcag aagggagtta cctctaagga
    22441 tctgattatg tacattatat cgcagatctc aaccagcggg gcaacaggat attttgttga
    22501 atatgccgga caggccataa ggaagctcag catggaagcc agaatgaccg tatgcaacat
    22561 gagtattgaa atgggtgccc ggggcggatt gattgccccc gatgaagaaa cattgcgcta
    22621 ttataaaaat tcaggggcat ttcctgaaaa ctttgactgg aaccagatga ctctgcagtg
    22681 gaaagacctg aaaagcgatc cggatgccgt atttgaccgc gaactggaat ttaatgctga
    22741 tctcatcagt cccatgatta cctttggaac aaatccggga atgggtgttg caataacgga
    22801 atcaattcca tcaattgaca aaataaacgg tgatgcttct gcatcattcc tgaaatcgct
    22861 cgattatatg ggattcaaac caggcatgaa actgttggga catccggttg attatgtatt
    22921 ccttggaagc tgcacaaacg gaagaataga agatctcagg gctttcgcca atattgtcag
    22981 gggcagaaag aaggccccaa atgtcaccgt actcatcgtt cctggttcca agatggttga
    23041 aaggcaggcc agggaagaag gccttgaagc catacttgaa gacgcaggtt tcaggctgcg
    23101 cgaaccggga tgctcttcat gcctcgccat gaacgaggat aaaatacccc cgcggaaata
    23161 tgcggtttca acatcaaacc ggaattttga aggacgccag gggcccggtg cgaggaccct
    23221 gctcgcaagc cctcttacag ctgctgctgc cgcaattacg ggaaaaataa ccgatccaag
    23281 ggaatttatc gactgaatgt tttgagtaat aaataagaaa tgtcaaagga aaaattcgaa
    23341 atactggaat caacttgtgt cccattgcca ctgcaaaaca ttgacaccga tcagattgtg
    23401 ccggccagat tcctgagtgc aacttcaagg gaaggattcg gcgaaaatct gttccgtgac
    23461 tggagatttg acaaggcagg taacctaaat cctcaattcg ttctcaacaa tccggagttc
    23521 ggcggtaaga ttcttgttgc cggcaagaat ttcggcagcg gatccagccg agaacatgct
    23581 gtctgggcaa tttatgatta tggattcagg gcggttgttt caagcttctt tgcagatata
    23641 tttaagaaca acgcccttaa taacggactc ctccctgtag tcgttcccga agaattcctc
    23701 agtgaactga tcgatctggt ccggaaaaaa ccacaaacca tagtaaggat cgatctcatg
    23761 aaccaggtta tttccatacc gtcagaaaac aggtcagccg gtttcgatat aaatccctac
    23821 aggaaagatt gcctgctgag aggactggat gatatcgatt acctcctgag tataaaggac
    23881 gagatcattg cattcgaaga aaagaataaa aaatgaaacc ttcatgattg accataaaca
    23941 ataacacaaa tgaatacagt cgcgttcagt catgtgcggt tccctgcccg aagggtcaga
    24001 cgggcatccg aaatttaaaa aattacatac ggatgaaaat agaaataatg gataccaccc
    24061 tgagggatgg tgagcagacg caaggtgtat cttattcacc gcttgaaaag cttcagattg
    24121 cctcgattct cctcaaggat gtgaaggttg accgcattga agtggcttct gcacgcgtgt
    24181 cctctggcga gttcgaggct cttagaaaga taacggaatg ggccaggcag aacaatatac
    24241 agagaaaggt agaggtactt ggctttgtcg acggaaatgt ttcattgaac tggatacacg
    24301 atgcaggcgg caaggtaata aaccttctct gcaaaggatc gctgcgtcac cttgagaaac
    24361 aattgcggaa aacccctgaa cagcatgtaa atgacataag ggagatcata gcaagggctg
    24421 aaaagctcga tatccaggtg aacatttatc tcgaggactg gtcaaacggc atgatccatt
    24481 cggaggatta tgtatattac atgatcgaca accttaaaaa tgaaacaatt gcaagattca
    24541 tgcttcccga taccctggga atacttaatc cggaactgac ttacagcttc attggaaaaa
    24601 tggttgaacg ttatcccgga cagcattttg atttccattc ccacaatgat tacgatcttg
    24661 cagttgcaaa tgtgttttct gccgttaaag ctggtgccag gggcgtgcat accacagtca
    24721 acgggctcgg ggaaagagcc gggaatgcac cgctttccag cgttatcggg gtcttgcacg
    24781 atcagctgaa tattgagacc aatatcaatg agtcggaaat catcaaaata agcaagattg
    24841 tcgaaacttt cagcggggtc aggatccctg tcaacaagcc tgttgtgggc gaaaatgtat
    24901 ttacacagac ttcaggggtg catgcagacg gagacaataa agccaatctt tatttcaata
    24961 accttcttcc tgaacgattc ggaagaaaaa ggaaatacgc cctcggaaaa caatcaggca
    25021 aagccacaat aataaagaat ctcgaagagt tcggactgaa cctcagtccc gaatccattg
    25081 ccaaaattac aaaaagggtg atcgaactgg gtgacaaaaa agaaaatgta acaaccgagg
    25141 atctcccttt tattatttca gacgtacttg gaagcgacag gtcggaattc aaaatattca
    25201 ttaagaatta ctccctgtcc ctgtcctacg ggttaaagcc ctcagcaagc gtattgatcg
    25261 atattgatgg caagctttac caggaaacct ccccgggaga cggacaatac aatgctttca
    25321 tgaaggcgct caggaagatc tatgacaagc tgggcaaaga acttcccgta ttgaccgact
    25381 atgaagtttc aataccaccg ggaggcaaaa ccgacgctct cgttgagact ttaatatcct
    25441 ggaacttcag gggcagggaa ttcagaacca gggggcttga tgccgatcaa accgaatcgg
    25501 caataaaagc aaccgagaaa atgcttaaca ttgttgaagg aatcaacgga acggaaaaga
    25561 atcacttatc agcctgagtc ctggcaataa acaggaacct gttcagaaaa gtcaatcatt
    25621 aacaaataaa caatctatgg ccagaaccta caatattgca gtaatccccg gtgacggaac
    25681 gggtccggaa gtggtgaggg agggaaaaaa ggccctttcg gctgccgctt tgaaattcgg
    25741 attcggaata aatttcactg agtttgattt cggaggtgac agatacctta agacaggtga
    25801 gattttaccc gattcagctg ttgcagaact cagtaaattt gattccatct tcctgggagc
    25861 aatcggtcat cctgatgtga agccggggat ccttgaaaaa gggatcctgc ttaaactcag
    25921 gttcgaactt gaccagtacg taaatcttcg cccgataaag ctctaccccg gagttgaaac
    25981 accattaaaa aacaagggtc cggagcacat tgattatgta gtggtgaggg aaaatacagg
    26041 cgacgtgtat accggtagcg gcggggtcac catgaagggg acccctaatg aggttgccat
    26101 gcagaatatg gtttataccc gtttcctggt ggaaagatgt ctaaggtatg catttgaata
    26161 caccaggaaa agaaacagga aaaaaaccct tgcactctgc ggcaagacaa acgtactcac
    26221 ttatgtgttt gatctatggg aaagagcctt caatgagatc ggcagtgcag aattccctga
    26281 tatcaaaagg gaatattacc atattgatgc aacctgtatg tggatggtca agaatcccga
    26341 atggttcgat gttattgtca ccacaaacat gttcggcgac atcataaccg atttgggagc
    26401 cataacacag ggcggcatgg gaatagcagc aggaggcaac atcaatcctg acggggtctc
    26461 aatgttcgag cccattggcg gatcagcacc aaaatacacc ggaaagaatg tgataaaccc
    26521 cctcgcatcc attgcatgcg gcggaatgat gcttgagcac cttggggaag aaaccgcagc
    26581 ccgtgcaatt gaaatcgcca tcatggatgt tacagcaaat aaactgaaag acatggccgc
    26641 aggaaaaatg ggttattcaa ccagcgaggt gggtgacctt gttgcagaaa ggatctgatc
    26701 actgccgcct gaatccctgc taacctgaaa agattcaacc ccacaggtag atccgtttca
    26761 aatgaaacaa acctaggctg aagaaaaggt ttccggggtt tattgatgat aattattaaa
    26821 aaatttctat aattgcatca ttatttttaa aaaatggatc tgattaaact gcatcatcat
    26881 tttcttactt gctctcaggc gagattgaaa tgatatgtca gtgcatcaaa aatatcatga
    26941 acccgtctga gaaaacaggc gggttttttt ctgccattcc cggccagatt agtaaaacat
    27001 ggagtatgaa gaaacttaga attgcaatcc agaaaaccgg aagactgagt gaaaactcac
    27061 aaaagctcct ggaagaatgc ggtattaaat tctcaaacgg agcaaatgta ctgaaaactt
    27121 cagccgtgaa tttccctgtc gagattttgt tcctgagaga tgatgacata ccgcagtacg
    27181 ttgaacagca ggttgccgat attggaatcc tcggggagaa catggttctt gaaaaagaca
    27241 aggatgtgga aattcttgaa cagctgggat ttgcccattg ccgcctcagt ctggcaatac
    27301 cggcgcagca tgatttcacc gggccggaat atcttatgaa caggaagata gccaccagtt
    27361 atcccaagct tctgaccgat tacctgaagg aaaacaatat tactgccgaa attgaagagc
    27421 tgagcggcag cgttgaaatc gcccctggaa tcggactggc agatgcggtt tgcgatattg
    27481 tcagctcggg aagcaccctg atgacaaacg gactgaagga ggtggtgaca attttaaaaa
    27541 gccaggcggt tttgattgca aacaaaaatc tggatcccga aaggatcggc atcgtgaaca
    27601 agctgctgtt caggatcagg tctgtaaaaa acgcgaagga aaataaatac atacttctga
    27661 atgctcctga accggcaatt gcggaaatct gcaaaatcct tccgggcatg aagagcccga
    27721 cggttctgcc gctggctgaa aaaggatggt gcagcctcca ctcagtggtc aaggaagatg
    27781 agttctggga aaggataagc cagctgaggg aggccggagc cgaagggata cttgtgatac
    27841 caatcgaaaa aatgatactc tgatgagaac gattgtaaat cctccctcct cagcctggaa
    27901 cgatctttgc agaaggcctg tcaaagatca gtcacaactg gccgtgcagg ttgggaaaat
    27961 cctgtctgat gtcaaaaaca acggagacag agccctgctg aaatacaccc tgaagtttga
    28021 cggggcagca acagatgacc tgagaatctc tgaaaaagag atatcggaag ctgaaaaaga
    28081 gattcccgat gaactgaaat ccgcaatagc cgttgccggt tcaaatattg aaagattcca
    28141 cagaacgcag cttccggatg atggtattat cgttgaaacc ctcagggggg tcagatgctg
    28201 gagaaaaagt gtagctgtag aaaaagccgg aatatatgtc cccggaggat cggctccgct
    28261 gttttccact gtcctgatgc tggccattcc ggcaagaatt gccggatgca gggaaataat
    28321 aatgtgcact ccgcccgaca aaaatggcaa agtaaaccag ttgatcctgt atactgcccg
    28381 gatctccgga gtaacttcat ttttcagggt tggcggagcc caggcgattg cagccatggc
    28441 ctacggaaca gaaacaatac caaaagccga caggatcttc ggcccgggaa accagtacgt
    28501 cacaaaagca aaggaaatgg tgcagctcga cggtgtcccg attgatatgc ctgcaggtcc
    28561 cagtgaagtt cttgtcatag ccgatgattc cgcggaaccg tcatttgtag cttccgacct
    28621 gatcgcccag gccgaacacg gaactgacag ccaggttctc cttgtaacca caagcactgc
    28681 acttttgtct gaagtcagta aggaaatcga caaacagctt ccggcacttc ccagaaaagc
    28741 gattgcagaa aaagcacttg caaacagctt gcttatatct tttgattcta ttgaaaaatg
    28801 tatggaattc agtaacctgt acgccccgga acatctaatc atctcagttt ctgatcccgc
    28861 tgctgcaaca gaatgcgtaa ccaatgccgg ttcggtattc atcggaaaat acagctgtga
    28921 aagtgcaggt gattatgcca gcggaacgaa ccataccctt cccacaaatg gcttcgcaag
    28981 aaactatagc ggtgtctcgg ttgacagttt catgaaaaaa ataacgttcc aggaactttc
    29041 cggggaagga ttattaaacc tgggaccggt cattcagaaa atggcagccg ctgaattact
    29101 tgaaggacac aggaatgctg ttacaatccg tcttaattcg cttaaaaatg cttgatatca
    29161 acttacttgt cagggacaat gtcaggaacc tgatcccata ctcatccgca agggatgaat
    29221 tccggggcag acatggcata ttcctggatg ccaatgaaaa tccctacgga aaattaaaca
    29281 ggtatcccga tccgtaccag caggaactga aattgcagat cagcaggata aaacagatgc
    29341 cagccgaaaa tatattcctg gggaacggca gtgacgaaat aattgatctg gctttcagaa
    29401 ccttctgcag gccgggtatt gacaaggtgc tcacatttcc tcccacctat ggtatgtatg
    29461 aggtctcggc aggaattaac gatattcctg tcattaaagt accgctgaca gacaaattcc
    29521 agataaacat ggaaaagact gttcctttac ttgatgaccc gcatctgaaa ctgatattca
    29581 tttgcagtcc gaataatccg accggcaacc tgcttaattc cagcgatata aaatatatca
    29641 ttgaacacag caggggtatt gtgctgatcg atgaagctta taatgatttc tcaggaaaac
    29701 aatcgttcaa tactatgata gccaggtatc ccaaccttat cgtaatgcag actttcagca
    29761 aggcatcagg actggctgct gcaaggatcg gaatggcttt tacagatcag aaaacagttc
    29821 agtatttcaa taaacttaaa cctccctaca atataagttc cctgaaccag aaagctgctg
    29881 ccggtcagat gactcattat gaaaaaacac ttgaccaggt aaggaaaata atcagcgaga
    29941 gggacagact gaagacggta ctgggcagcc tgaaaattgt tgaggaaata tatccctctg
    30001 atgcaaattt ccttcttgtc agggtaaaag atcctgatta tatttataag tcacttgcag
    30061 ccagtaatat aattgtgcgt aacagaagcc gggtcgttcc gggctgtctg aggataacca
    30121 taggtacaaa acctgagaac aacaggctga ttgctgcact gaagcgtatc agcatatgac
    30181 cctgctactg ccttaaatga aaaaaggaaa aaatgagact aacaggactt acatgcaccg
    30241 ggtggttcgt aagacaagat gaatatctat gaaatccaca tgatacaaaa tcacaaacaa
    30301 gcatgaaaat aaaattcatg tggattccat gtgaaccact taatctacag tagtatataa
    30361 attaattttc acatgaaaaa ggtattgttt atcgacaggg acggaacaat aatcaccgaa
    30421 ccccttgacg aacaggttga cacttttgaa gatctgaggt ttttacccgg agtgatcacg
    30481 gcattgtcga aaatagcagc cgaaacagac ttcgaactgg taatggttac caatcaggat
    30541 ggcctgggaa cagcatcatt cccggaggaa agtttctggc cgcctcacaa caaagtcatt
    30601 cagatccttg aaggagaagg tgtcaggttt gcagaggttt ttatcgacag gacatttccc
    30661 gaagacaatg ccccgaccag gaagcccgga accgcgatgc ttacaaaata tctcgcctcg
    30721 ggaatcgacc tggaatcatc atacgttatc ggcgaccggg tgacagatat ccagcttgca
    30781 aagaacctgg gatgcaaggc aatatttata ggcgacccaa atcaacctga cgcagtcctg
    30841 tgtaccaggg attggaatga tatctacagg ttcctgaaag atattccgag aactgcgatc
    30901 atcgaaagaa agacaaatga aacctcagta agcgtttacg tcaatcttga cggtaccggg
    30961 aaaagttcag taagtacggg gctcggcttc cttgaccaca tgcttgatca ggtagccagg
    31021 cacggtacgg ttgatctaac cataagggcc gcaggcgatc ttcatgtgga tgaacatcac
    31081 actgttgaag atgttgcaat agccctcggg caatgtgttt caaaggccct tggcaggaaa
    31141 aaaggcattg aaaggtacgg attcgtcatt ccaatggacg attcccttgc cgaggcagcc
    31201 attgatttcg ggggaaggcc ctggcttgcc tggaatgtca gcttcagccg cgaaaagacc
    31261 ggtgatgttc ctacagagat gtttccccat ttcttcagat cattctgcga cagtgc
//
LOCUS       DAOOYG010000019        31174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1237_length_31174_cov_6.511906, whole genome shotgun sequence.
ACCESSION   DAOOYG010000019 DAOOYG010000000
VERSION     DAOOYG010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 31174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31174
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1237_length_31174_cov_6.511906"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="PK288_03180"
     CDS             <1..108
                     /locus_tag="PK288_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HPM17952.1"
                     /translation="YRFIQGDGILVFILAPGAFIALGYFIALVNRVKNK"
     gene            121..696
                     /gene="rsxA"
                     /locus_tag="PK288_03185"
     CDS             121..696
                     /gene="rsxA"
                     /locus_tag="PK288_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HPM17953.1"
                     /translation="MEYILIIISAVFVNNIVLAQFLGICPFLGVSNKINTAVGMSGAV
                     TFVIVLATIVTYLIQVFILDKLGIGFMQTITFILIIAALVQMVEIILKKISQPLYQAL
                     GIFLPLITTNCAVLGVAILVIRKNYNLLEGVVYSAATAVGFGLALVILAGIREQYDLL
                     DIPKGMKGVPISLVTAGILALAFMGFTGIVK"
     gene            797..1393
                     /locus_tag="PK288_03190"
     CDS             797..1393
                     /locus_tag="PK288_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPM17954.1"
                     /translation="MTYLIMGLSEYYEILGCPVDSSVAEIKRTYRRKARQYHPDVNHE
                     PGAIDKFIKLTEAYDFIIANHDKIPWGDQEFERIMDEWRKFRQERSRERARYYARSSY
                     TRFKNSKYYKSTKILNASSVIFGFCISLLVLVYTIAGYIFRVRHPLDGIKNPSVLTFI
                     ILLIFSIVLIIVSSIYLKAYIEINRKQKRKQADGRNSP"
     gene            complement(1475..1864)
                     /locus_tag="PK288_03195"
     CDS             complement(1475..1864)
                     /locus_tag="PK288_03195"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPM17955.1"
                     /translation="MKEYRVAATNTSPEIVFDPGGIFRISGRSIHENMSAFYKPAEDW
                     LDEYINDPADITCLEINLEYFNSASSKLLVRLMQKISGVELRKKKFIVNWYYEDGDED
                     ILERGEYFSSVLKKPFNFIKSSGSWHG"
     gene            complement(1908..2942)
                     /locus_tag="PK288_03200"
     CDS             complement(1908..2942)
                     /locus_tag="PK288_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HPM17956.1"
                     /translation="MKSHDLSFLVLGAGAVGGITAALMKVKGYNVEVICKYDDYASLI
                     SGQGINVKGVRGNFTVQLPAYSSAVKLKEKKDVILHATKATDMTQAAMEAMPLLKDEG
                     CFVSMQNGICEDELIRVAGNNRVIGCVTGWGATMESPGNLIMTSSGDFILGYPDREPD
                     EFLNLVAEALSSVVPVRMTENIMGHLYSKLIINSCITSLGAICGLYLGKMLSMEKVRR
                     IFIEIIRESVLVADSMKIRIETFGGKLDFKKFIAGTGPLADLKRHLMIRLIGFRYRRL
                     KSSSLQSLERGKQSEIEYLNGYIVRNAEKYNIEVPVNKAVVRLIHEIEAKKREISVNN
                     FSDPAFERFN"
     gene            complement(2960..3973)
                     /gene="tsaD"
                     /locus_tag="PK288_03205"
     CDS             complement(2960..3973)
                     /gene="tsaD"
                     /locus_tag="PK288_03205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPM17957.1"
                     /translation="MAVIILAIESSCDDTSVAVIRDGYILSNLIAGQEVHMMYGGVVP
                     ELASRAHQANIVPVTDVAIRQAGISRKDINAVAFTRGPGLLGSLLVGTSFAKGVALAL
                     GIPLIEVNHLQAHVLAHFILEEQAGHQQPSFPFLCLLVSGGHSMLIVLKDYLEMEIIG
                     NTIDDAAGEAFDKCGKLMGLPYPAGPAIDRQAKQGNRTAFRFAKPFVEGLDFSFSGLK
                     TSFLYFLRDRTKEDPDFISRNIPDLCASLQATIVDILIGKLVRASLQTGIREIGIAGG
                     VSANSELREALTREGIKRGWNTYVPPLGYTTDNAAMIGITGYYKYLRGEYVGHDVTPM
                     ARL"
     gene            4037..8506
                     /locus_tag="PK288_03210"
     CDS             4037..8506
                     /locus_tag="PK288_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPM17958.1"
                     /translation="MLKSIKKSFKYLVILAGIIMLVPTSTYLLLQIPEVQTYIVKRIT
                     AHFSDQIKSTITVGRFEYAFFNKLILENVLIKDKNLDTLLYSGKVTAGIRKLDLRNSN
                     IRFGRVRFDRPLVALITDTTGLMNLNWYLDMLASSDTSSAGKKSRISIDQAELNDARF
                     NLIDRRSRPGKSLIDFGNLHVGRISAEVRNFTVKDDSVNFRIARLSFIEKNGFAVENF
                     AGRVTLADHDILFNDIQIKTGKTDLKADNVYLTADSAGSFARFNERVRLDILLRRSIV
                     SSADLQYFLPFLEGTDETGEVSGRVTGFVEELKGRNINVRYGAGTLLDCDFDFSGLSS
                     IQDAFIHLGINTLVTSASDIERISLPGKGKGKIRLPEVMHKLGQITFDGSFTGFVSDF
                     VTYGKIGTEKGHIRTDLSVRPWQGSRFKIKGLIAGSGIELGDLTGNRDLLGKMSMETS
                     IDGFASSMEKIEGDLTGVIDSIQINNYLYRNVAINGLFTEKTWDGSISISDRNIRMDL
                     LGLFDFRNALPEFDFTLNLAESNLYELNIDKSDSTSALSMLVTANFRGNNIDNLYGEI
                     KLLNSTLNKFGNKLELYNFSLKAFTEANRPAISLRTDFIDADLRGYYNFGDLNNVIKS
                     ALASLMPARFPVPYAGKVQIKNDFNFSVNFKNTDKINAFFRTGVFIADKSTITGEIHP
                     DNNLRLGIMSKQLAIKNYSLSNLTFDADCQEGKLNAELKSTVLSLLGETELKDFTSTL
                     STLPDNFLFNLHWDNKDRIKNKGNITAVGNFDKNENQRPVMHISIDSSELYTRDNLWK
                     IKRSEILIDTTSVDFNRIMIVNGNNYYLVDGTISEDPTDTLRLEFVGIELDPLNHLLS
                     GSNDGKENLDYGFRGTLNGGIFLTNILKNPLLEGDLKIAGFSVLGSEYGDLSLEATWN
                     SERKVADISASNNLRGEKNLDVKGIYDPGDKLFNISAKASKLPVDALNPLLDFFASDI
                     RGTASGKVNLRGEKGNLFLTGALMAENTSLMVDYLRTRYRLNDTIRFDRNGIVFRNIK
                     LTDERGNLATISGSVNHKSFKDFTADLIINMNDCMVLNTLAKDNDLFYGTAYARGVTT
                     IKLRPDLLSFDISARTGKNTRFFIPLNSGMSVSESSFITFMASDTSRLNDHHRPDRIL
                     PSAAAGADLELNIDLDVTPEAEVQLLIDPKAGDVIRGRGTGQLNINLNRKKEFRISGD
                     YIIEEGDYLFTLGNVLNKRFDVENGGKITFNGDVENAEIDLKAKYRNLKTSLYPILGD
                     ERYNERIPVEPQLNLSGRLFNPVVGLNIYLPNADEETRSLLQNSITTEEELSRQFLYL
                     LVMNSFYNDPSMGISSVSTTSTGTSAMAVTTTEMLSNQLSNWLSQIYNDFDIGFVYRP
                     GNKDINSQELQVALSTQLLNDRIVVNGNFDIRGANNSYGNPITGDFDIEYKITEKIRF
                     RAFNRFNNPYTGRGAPYTQGLGLFYKQDFNKLFRPKTKSQTSDMKKEDEIVVR"
     gene            8656..9348
                     /locus_tag="PK288_03215"
     CDS             8656..9348
                     /locus_tag="PK288_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPM17959.1"
                     /translation="MIFLQNVMQDVPGTVQMMSETKMTLLDFAIKGGWVMIPIVLLSL
                     VAAYIFFERYYVINRASREDTNFMNRIKDYIHDGKVESALALCKSTEGPSARMIEKGI
                     SRLGRPLQDISTAIENVGKLEISKLERGFPTLATITGAEPMLGFLGTVIGMVQSFYAM
                     SQAGNNIEVSLLSNGIYTALITTVAGLIVGILGYFAYNTLVVRVEKVVFNLEATLTEF
                     MDILNEPLKTGR"
     gene            9350..9742
                     /locus_tag="PK288_03220"
     CDS             9350..9742
                     /locus_tag="PK288_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPM17960.1"
                     /translation="MAISSRHKISINFSSTGMTDVVFNLLIFFMLTSTLVHPTALKLL
                     LPKGSTQTSAKPQTTVSITADQKYYVEQQPVTLEALESVLKQKLGANPETYISLHADK
                     NVPFETVVAVLNIAQANNYKLIIATTPR"
     gene            9723..10616
                     /locus_tag="PK288_03225"
     CDS             9723..10616
                     /locus_tag="PK288_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPM17961.1"
                     /translation="MQPHPDNRLRSIPHEKKKGFIGTLIIHVVLLVLLLVVAFIPPKP
                     PELEEGILVNFGTDETGMGQIEPSAPAAQPETTPPPPATLPKVQADEPLLTQNNEEAP
                     EVKKVDPEAEKKRLEKIEADRKLREQMEAERKRREQEEAERKRIEAEQKRQAEIMNKT
                     REALAGSKNAGTMAKSEGDAGGSGNQGDPGGDPGSRNRGQGSGLGDRGISFFLEGRGF
                     QSLPSPRYDYQGEGKVVVEVSVDRSGNVTQATPGVKGSTTLDEYLLRVAREAALKARF
                     DPKPDAPVTQKGTITYIFKLN"
     gene            complement(10626..11909)
                     /gene="glyA"
                     /locus_tag="PK288_03230"
     CDS             complement(10626..11909)
                     /gene="glyA"
                     /locus_tag="PK288_03230"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPM17962.1"
                     /translation="MERDNTVFDLIEKERQRQINGIELIASENFVSPQVLEAMGSVLT
                     NKYAEGYPGNRYYGGCEIVDQTEMLAIARLKKLFNAEYVNVQPHSGAQANMAVFLAVM
                     KPGDTFLGLNLSHGGHLSHGSPVNSSGILYRPVAYSVREDTGLVDYDMMEELAMKEKP
                     KMIIGGASAYSREWDYARMRKIADSAGAILMVDMAHPAGLIAAGLLKNPLEYAHIVTS
                     TTHKTLRGPRGGIILMGRDFVNPWGLTTPKGEPRMMSAILNSAVFPGIQGGPLEHVIA
                     SKAVAFGEALKPEFKDYQVRVQRNARVMAEAFMNRGYKVVSGGTDNHLMLIDLRTRFP
                     ELSGKKAENTLVLSHITVNKNMVPFDSRSPFLTSGIRLGTPAITTRGLKEEHMGIIVE
                     MIDEVLSNIDDESVIRRVGDRVVSMMKSFPLYSAG"
     gene            complement(12032..13813)
                     /locus_tag="PK288_03235"
     CDS             complement(12032..13813)
                     /locus_tag="PK288_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPM17963.1"
                     /translation="MFHGNFFEKLKNLELFRIDQVKIIYSLSLVVGLISALAAAFLKN
                     AIHYTNRIVTEGLTQQSTGHLYLLFPVCGMLLTMLFVKYIVRDNIGHGVSRVLYAISK
                     KKSRIKKHNTWTSLVATTLTIGSGGSVGSEAPIVLTGSAIGSAIGEYFKLNYRNITLM
                     LGCGAAGAISGIFKAPIAGIVFTLEVLMLDLTISSIVPLLISSVTAATVAYFLMGNEV
                     LFSFSLTDVFMIRYIPWYILLGVVSGFVSLFFSKMTLLLERRYEKIRNDYVRLIAGGT
                     TLAALIYLFPAFYGEGYDTIRLLLQGKTADVFGNSILYRFSDNYLVFILFFFCMVMLK
                     VVATSSTNGGGGVGGIFAPTLFIGGVNGFFIAGILEKYIGVDISENTFILAGMAGVMA
                     GVMHAPLTAIFLIAEMTGGYSILITLIITSTVAFITTRSFEKHSIYHVQLAESGDLIT
                     HDKDKAVQTIMDWKKEIERDLIKVRNSDSLGDLVKVIARSKRNLFPVVDRYNILEGVV
                     LLDDVREIMFNTGLYDKVSVKEIMSMPPSYIDISEGMDTVMKTFSETGAWNLPVLDKG
                     YYVGFISKSRIFSAYRDLLAQFSDDAG"
     gene            13906..15150
                     /locus_tag="PK288_03240"
     CDS             13906..15150
                     /locus_tag="PK288_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPM17964.1"
                     /translation="MIDFVKFSLRNGLRILVHEDRSTPLSALNLLYGVGSRDEDPEMT
                     GIAHLLEHLMFCGTENVPVFDRPLQMAGGENNAFTNNDITNYYLTVPADNIETGFWLE
                     SDRMNGINFSRDALDVQKKVVIEEFKQRYLNQPYGDSQLLLRPLAYTTHHYKWPTIGK
                     SISHIESIEPEHARDFFYRHYAPGNAILTVSGNISPDRVFKLADKWFGPLEKRTTRPQ
                     VIPAEPFQTEERNLIVERDVPVDALYKAWHICGRKEDDFFTFDLLSDLLAGGESGRLH
                     SKLVRDKNLFSDINAYISSDIDPGLLVIHGKLMKGVTITEAEENVRMVVDELKSGIIS
                     DEEMEKVKNRFESSHVISNTSILNKAMNISYYELLGNPGLINLEVERYRAVSREMVRD
                     AAIKYLNNNNSSTLHYLSKNRN"
     gene            15153..16427
                     /locus_tag="PK288_03245"
     CDS             15153..16427
                     /locus_tag="PK288_03245"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPM17965.1"
                     /translation="MSAEKMTAPSINPINPGSLAPVKSIVLGNGIPVHIIEGGTEDVM
                     RIEFILRAGTVMENIPLQASSTNMMLTEGSEKYSSEEIKALLDGYGVFYQLSAERDSA
                     GLIIYLLNRHAEKAIELIAEILFNSMFPEKEFVIMMKKRLRRFMISREKVQTLASEKF
                     FETIFGNNHPYGKMVSENDFEALNTDHLKNFYASYYTPANLSVIVSGKINENIQEIMD
                     KYLGSKLPGTPLQAGNESDFQKCPQKRIHVPKKGALQSAIRIGSATINKRHPDYPGLK
                     VLNTILGGYFGSRLMKNIREEKGYTYGIRSVAASLNLSGYKIISAEVDNQSVREAIAE
                     IYREISKLQKEPVAYDELELVRNYMAGEMVRMFDGPFSMAESFRAIWEFNLEADYYRK
                     LAHTVRTITPDELLHLANKYYNIDELYEIVAG"
     gene            16399..17910
                     /locus_tag="PK288_03250"
     CDS             16399..17910
                     /locus_tag="PK288_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM17966.1"
                     /translation="MNYMKSLPDNRLSTVTGLMILLMSSVLFTVNCQEDTGPQPPVFS
                     LVSINQTTGNTEMSWTPSLSPGVRGYIIYLYQNGDGFKIDSIFNPLQTTYSVYRAWSS
                     VRSESFGIAAIADTLSPISNILKTIFCEAAADSCKKNIKVTWNKYISFPTAVTGYDVL
                     RSVNGGPFSIAGQTQNSDTIFVTDDYENGTEYCFAVRARLSDGKSSYSNKACVTVRIT
                     RGPDWINADYATVTESDVISLSFTIDPSSETDLFTLERKTGPSGSFKEIASIRTSVNT
                     VTYIDDDGNPNHNNYYRLSAINSCNLPDKTSNIASNILLKYERGPDENIILKWNSYYK
                     WNGVIGSYILYMDQGSGFFQHSLLSPSDTTCLISIPEVMFGAYGEKICFYIVAGETAN
                     PYGINGESRSNTTCFKLEETITVPNIFTPNGDARNDLFAPVLTFTPLEYHLVISDRHG
                     RVIFETRDFTESWDGSDRNNPPREGVYIWSLRVLTNNGNKISKTGTVTIYKNK"
     gene            18003..20264
                     /locus_tag="PK288_03255"
     CDS             18003..20264
                     /locus_tag="PK288_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPM17967.1"
                     /translation="MGNKDIFDEEDIKRIQDEYDSLINNLSKCNKPEDRELIEKAFQV
                     ANKAHWNMRRKSGEPYILHPIAVAKIVNQEMGLGTRSIVTSLLHDVVEDTEYSIEDIS
                     REFGPKIASLIDGLTKISGTYNKGSSSSMQAENFRKMLMTLSDDIRVILIKIADRLHN
                     MRTLDSMPENKKLKIAGETIFLYAPLANRLGLYAIKSELEDLSFKFRQPQVYEEIATK
                     LKHGEQKYLALINKFSLPIIDKLTESGLQFEISGRPKSIFSIWKKMQAKNVPFEEIYD
                     VLAVRVVFEPIPGIPERTTCWSIYSAITDVYLPKPDRLRDWISRPKPNGYEALHVTVM
                     GPEGRWVEVQIRSRRMDEIAERGYAAHYKYKGDNSPETELDKWIRKIRSMLENPLEDP
                     IEFLDEFKMNLFSSEIMVFTPKGALVSLPKGASALDFAYEIHTEIGNKAIGAKINYKL
                     NPINAILMSGDQVEIITSDIAKPEKEWLSFVRTSKAKEAIKNQLRTETTNRIQKGKNI
                     LASKLSELGVVPNSEIVRKLMLSYDLFNKDELYSGIELGTISLDNLKKIIRKTPAKNV
                     IKYWELKLLGSMKGKKEEEHEHPEKIDDGAPFLLRENVEDAGQSYEIAKCCNPIPGDE
                     VIGYRSPKGAIIIHKPKCPVAIRLMANEGNRIIAVKWAIHKLVSFLARISMTGVDRIG
                     FVNDITNIISSELKVNMTNINISVKDGILEGTIDLYVHNTQDINNLILKISNVRGIVS
                     IKRVEDFSEQDAN"
     gene            20315..20761
                     /locus_tag="PK288_03260"
     CDS             20315..20761
                     /locus_tag="PK288_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008653150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPM17968.1"
                     /translation="MADDETKSTVKQIFTEYLEKKGHRKTPERYAILDEIYSQSGHFD
                     IESLYVFMKNKNYRVSRATLYNTIELLLDCNLVIRHKFGRNLAQFERAYQCSQHDHLI
                     DVETGKVVEFCDPRIRNIIKTASTLHNFEPAYHSLYIYGSFKEKNK"
     gene            complement(20843..21496)
                     /locus_tag="PK288_03265"
     CDS             complement(20843..21496)
                     /locus_tag="PK288_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17969.1"
                     /translation="MKDLKVFLIPVLILLFTANNLSAQKSEGEKDFRFTIKTNPLSAL
                     GGPFWVIVVPLTGEYKAFFEAKVSEKTSIQVGAGYIGPSVLLNLDKITSQDESEGIQG
                     IKTGGFRVQGMFKYFLSRDLSAPEGFYIGPHASYASVEIKNKQNDVEKIKASKLNLNG
                     IFGYQLITSGGFALDVYTGLGFVSRNWDLQGSDWDEDVFKNRIGVNVPFGFTFGYAF"
     gene            21688..22950
                     /locus_tag="PK288_03270"
     CDS             21688..22950
                     /locus_tag="PK288_03270"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPM17970.1"
                     /translation="MKIDILLGLQWGDEGKGKIVDALSPGYDVIARFQGGPNAGHSLE
                     INNVKHVLHLIPSGIFHPGKLNIIGNGVVLDPAVFRQEIEHLGIPLDELRKRLIISSR
                     THLILPTHRILDAAYENRKGNHKIGSTLKGIGPAYTDKASRNGIRTGDLLREDFTRLY
                     EEHVNCHIELLKNLDHSFDLKAYEQGWFEGIELLRSFRIDNTEYLINELASKGKKILA
                     EGAQGTMLDLDFGSYPFVTSSNTTSGGACTGLGISPRLTGEVFGIFKAYCTRVGSGPF
                     PTELSDSTGERLRERGHEYGATTGRPRRCGWLDLPALKYAIMINGVTRLFMTKSDVLS
                     GFGTIKVCRSYIRGNKESMEVLADSNIETGLIYEEFDGWSEDISRIREFSQLPASLRR
                     YIEYIEQETGIPVSLVSVGPGRNETIFR"
     gene            22992..24905
                     /locus_tag="PK288_03275"
     CDS             22992..24905
                     /locus_tag="PK288_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM17971.1"
                     /translation="MKSLIQFFEESVDKFNVNVYLWEKPVDRYEGTTYGEARKQVYEF
                     AAGLLKLGIKKGDRLSLISEGRNSWVIGELGILYTGAVNVPLSVKLSPEEIKFRINHS
                     GSRMILTSSIQSQKVKQIINECPSLEKIIHFDALEEYEENEIHFNEVRKLGREWLESP
                     ENKSLFDEISKNIKPSDFANICYTSGTTADPKGIILTHGNYVTNVYQAYSLMDIPSFY
                     KTLLILPWDHSFGHTCGIFAFMGKGASIASVKTGKTPLETLRNLPSCLREIKPNLLMS
                     VPAIAKNFRKNIEKGIKEKGRLAEMLFNHALRISYKYNKEGWNKAKGLQKLKKPLINL
                     YDKILFSKIRESFGGELDYFIGGGALLDIELQRFFYAIGIPMYQGYGLSEASPVISSN
                     STARHRLGSSGVLVNNMDLKICDNDGKEVPLGEKGEIVIRGGNVMHGYWKNETATRET
                     IRDGWLYTGDMGYMTKEGYLYVLGRFKSLLIADDGEKFSPESIEEALTEQSKYIDQCM
                     LYNNQKPYTVALIVPNQHALKVYLEGRDLTADTDEGKRAVLTLIEDEVDEYRTHGKYG
                     NMFPQRWLPVSLGILEEGFTEENGLMNSTMKIVRGKIMDRYQELIEYLYTPDAKIITN
                     ERNIEEVEKMKLG"
     gene            complement(25227..25388)
                     /locus_tag="PK288_03280"
     CDS             complement(25227..25388)
                     /locus_tag="PK288_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPM17972.1"
                     /translation="MEKYKCSVCGYVYDPEEGDCISDIRPGIAFSDLPDEYVCPVCGA
                     GKEEFYALD"
     gene            complement(25544..25957)
                     /gene="mce"
                     /locus_tag="PK288_03285"
     CDS             complement(25544..25957)
                     /gene="mce"
                     /locus_tag="PK288_03285"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPM17973.1"
                     /translation="MKPTNIEHIGIAVRSLEESIRLYEAILGLKCQKIEEVSDQKVRT
                     AFFKIGTTKIELLESTDPEGPISRFIEKRGEGLHHIAFKVNSLKDKLSATKNAGIRLI
                     DEEPRPGADGLNIAFLHPASASGVLIEFCENRENR"
     gene            complement(26068..27627)
                     /locus_tag="PK288_03290"
     CDS             complement(26068..27627)
                     /locus_tag="PK288_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM17974.1"
                     /translation="MTSAFLRRLRIVFSAVVLVCFIYIFAEFRTLIQPEYIKYLLYLQ
                     FIPSIIKFIDIKSLAAGGFLIVLLLTLVTGRTYCSFLCPLGIGQDVFSRIGGTIRKKF
                     RRFGYKRPHTVVRYSVLVLVIAGTFIWGMYFINLLDPYSIFGRSILFFIKPVILVLNN
                     FLAGILGRFDNYALHHLEIKGYNLMAYAIPAGFFILTGLLSLTKGRLYCNMICPVGTF
                     LGLVSRLSLFRIKADENKCTRCGRCSVVCKSSCIDFLNHEIDVSRCVNCFDCIYSCPD
                     KALSYGFIGNTREEKKSDESRRRFIAGSIMLFLGLSGSGRAQERGIPVPRRESTVKEE
                     KNCPVSPPGAGSISDFNNRCTACGLCISVCPNDVLQPAIMQYGLAGYMQPVMDYHKGF
                     CQYNCTLCTEICPTFALKPLLPEAKKLTQIGKVIFIKDNCIVKTEKTACGACSEACPT
                     KAVFMIPYEGSLVIPDTNQEICIGCGHCEFSCPTVPYKAIYVNGNPVHQASKKPDIGK
                     PGVSVPDDFPF"
     gene            complement(27634..28554)
                     /locus_tag="PK288_03295"
     CDS             complement(27634..28554)
                     /locus_tag="PK288_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPM17975.1"
                     /translation="MKRRGFFKTSLGAGIAAGAALSLGRYNRVWASNGTGAKYDLVAV
                     MGGDPDEMFDLGIQELGGMGNFVSKGQRVLIKPNIGWDVTPDLAANTNPLLVKRIIEH
                     CLKAGAREVYIFDHTIDNWINTYRNSGIEKAAKMAGAKVAPANSEKYYHEITIPTAVK
                     LKNAKVHELLLETDVFINVPVLKDHSSTRITCAMKNAMGIVWNRGYWHANNLHQCIAD
                     YALFKKKPALNVIDCYNVIAKHGPQGVSKEDVVQMKSQILTTDWVAGDAAAARMLGIA
                     PEKIDYILIAHKMGIGNIKLESLNIKRIKM"
     gene            28721..29899
                     /gene="larC"
                     /locus_tag="PK288_03300"
     CDS             28721..29899
                     /gene="larC"
                     /locus_tag="PK288_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HPM17976.1"
                     /translation="MKVLCYDCFSGISGDMNLGAMLALGIEESYLIGELKKLNLDGWE
                     LLVEKDQRHGIAGIKATVRQKGHDHSHRHLSDIEKIISDSGLGEDVKKLSMRIFMKVA
                     EAEAHVHGIPVDRVHFHEVGAIDSIIDIAGAAICFSALKVDAVYVSEVELGGGFVVCE
                     HGTLPVPAPATAEIIKGIPVRKGGLDFEATTPTGAAILAALGTDFSKTHSLKILKTAY
                     GIGQKEHNDVPNLLRVSLGETTDSENTGHNAVHLECNIDDMNPEFYDYISEKLFSAGA
                     SDVFFTNIIMKKGRPGIILNVICETGHEDNLKELIFKESTTVGIRTVQVRKDTLVREF
                     TRLNTKFGEVTVKHSYLKGSEVSVKPEFDDVRRIAAENGIPVKDVYSMILSMISANRK
                     "
     gene            29903..30697
                     /gene="larE"
                     /locus_tag="PK288_03305"
     CDS             29903..30697
                     /gene="larE"
                     /locus_tag="PK288_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPM17977.1"
                     /translation="MFNNLLDKLDQILTELESFVVAYSGGVDSSFLLYRAHSIKKADV
                     IGVTIRTPYIPDYEINDAVEFVKIHGINHRIIDLSFPEIIRNNPVERCYSCKKTLFSA
                     MITFAAENGFRYVVDGSNADDINDFRPGLKALRELNIRSPLLEAGLTKKDIREIAFKH
                     KLPIWDKPAMACLLTRIPYNTEVNTETLRMIERAESFLFENGYPGARVRVHGDLARIE
                     CLPGFFERIIRSPEKELITENLKRIGFRYISLDLEGYRSGSSNPEK"
     gene            30702..>31174
                     /locus_tag="PK288_03310"
     CDS             30702..>31174
                     /locus_tag="PK288_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-amino-4-imidazole-
                     carboxylate carboxylase"
                     /protein_id="HPM17978.1"
                     /translation="MTRKEIEILLREVSKGEKSVDQAMEILRDFPYTDLGYARIDHQR
                     EMRTGYPEIVYCAGKTIEQVAGIFRVMSEKENNVVGTRANPEMYEAVRLLIPSAIYYP
                     EARIISVQKKKPVCPETRIAVITAGTSDIPVAEEAAVTAELLGNNVFRTYDAGVAG"
BASE COUNT         9295 a         6984 c         6835 g         8060 t
ORIGIN      
        1 tacaggttca ttcaaggtga cggcattctt gtgtttattc tcgccccagg tgcctttatc
       61 gcactcggat attttattgc actcgtaaac agggtaaaaa acaaataaaa gacaacagga
      121 atggaatata tactcataat tatttcggcc gtattcgtca acaacattgt cctggcgcaa
      181 ttcctcggca tctgtccctt cctgggggta tccaacaaaa tcaatactgc cgtaggcatg
      241 agcggcgctg tcacattcgt catcgtgctt gcaaccattg ttacctatct catccaggtc
      301 tttatcctgg ataagctggg tattggtttc atgcaaacca ttacattcat actgatcata
      361 gcagccctgg tacagatggt tgaaataata cttaagaaaa taagccagcc gttgtatcag
      421 gcccttggca ttttccttcc cctgatcaca accaactgtg ccgtcctggg ggttgcaata
      481 cttgttatcc ggaaaaatta caacctgctt gaaggcgttg tctacagtgc agctacagca
      541 gttggcttcg ggctcgcact cgttatcctg gccggaataa gggagcagta tgaccttctg
      601 gatatcccaa agggcatgaa aggagtaccg atctccctcg ttactgccgg aatacttgcc
      661 ctggcattta tgggattcac aggtatcgta aaatagcagt cgggactggc cggaatctcc
      721 cgcggaaatt gttttgcact atcttccgtg atcatgccgg aaaccggaaa atatttcgtt
      781 aattgtaggt aatttaatga cttatctgat aatggggttg tcagagtact atgagatcct
      841 gggttgtcct gtggattcct cagttgccga aataaaaagg acttacaggc gtaaggcacg
      901 gcaatatcat cctgatgtca atcatgaacc cggggcaatt gacaagttca tcaaattaac
      961 cgaagcctat gattttatca ttgcaaacca cgacaaaatc ccctggggcg accaggaatt
     1021 tgaaagaatt atggatgaat ggaggaaatt caggcaggaa agatcaaggg aaagagcccg
     1081 gtattacgca agaagttcat atacccgttt caaaaacagc aaatattaca agtccacaaa
     1141 gatattgaac gcatcttcag ttatattcgg cttttgcatt tcactcctgg tactcgtcta
     1201 tacaattgca ggttatatct tcagggtcag gcacccgctt gacggcatca agaatccttc
     1261 ggtgctgacc tttataattc tccttatttt cagcatagtg ctcataatag tgtcatctat
     1321 ttatctgaag gcatatattg aaataaaccg taaacaaaaa aggaaacagg cggacggacg
     1381 gaattctccc tgaaaccgaa agccacggat caatgtccgt tcaggataaa tgccagtgaa
     1441 cagtccgcag caggttattt cctgctcagg gttttcaacc gtgccaggat ccgctgctct
     1501 taataaagtt gaatggtttc ttcagcactg aggaaaagta ctcaccacgt tcaaggatat
     1561 cttcatcacc gtcctcgtaa taccagttga caatgaattt ttttttcctt agttccacac
     1621 ctgatatctt ttgcatcagc ctgaccagta gtttggagct tgcactgtta aaatattcaa
     1681 ggtttatttc aaggcaggta atatccgcag gatcattaat atattcatcc agccagtctt
     1741 cggccggctt gtaaaaggcc gacatattct catgtattga gcgtccgctg atcctgaaaa
     1801 tacctcccgg atcaaacacg atttcaggag aagtatttgt tgctgccacc ctgtactctt
     1861 tcattttcca aactgtattg gaactctaaa gtatcaatat tttactgtca attgaagcgt
     1921 tcaaatgccg gatcagagaa attattaacg gagatctctc tttttttcgc ttcaatttcg
     1981 tgaattaagc gcacaacagc cttgttaacg ggaacctcaa tattgtactt ctcagcattt
     2041 cttacaatgt agccgttcag atattcaatt tcagactgtt tacctctttc aagcgactga
     2101 agactcgaag atttgagccg cctgtatctg aatcctatca gtcttatcat caggtgccgt
     2161 ttgagatcag ctagcggacc cgtgcctgca atgaattttt tgaaatcgag tttccctccg
     2221 aaagtttcga tccttatctt catgctgtcg gcaacaagca ccgactccct gattatctcg
     2281 atgaagatcc tcctgacctt ttccattgaa agcattttcc cgagataaag gccacaaatt
     2341 gcccccagcg atgtgatgca agaatttatg atcagctttg aatacagatg acccattata
     2401 ttttccgtca tccgtacagg aacaaccgaa gacagagcct cggcaaccag attgaggaat
     2461 tcatccggtt ccctgtcggg atatccgagg ataaaatcgc cagaggatgt cattatcaga
     2521 tttccgggtg actccattgt tgcgccccag cccgttacgc agccaataac ccggttattg
     2581 cctgctaccc tgatcagctc atcttcgcag ataccgtttt gcattgaaac gaaacaacct
     2641 tcatctttaa gcaatggcat tgcttccatg gcggcttggg tcatgtctgt tgcctttgtt
     2701 gcatgaagta tcacatcctt cttttctttc agctttactg ccgatgaata tgcaggcagt
     2761 tgtacggtaa agtttccgcg tacaccttta acgtttattc cttgtccgga tatcagcgat
     2821 gcataatcat catatttgca gatcacttca acattatatc ctttgacttt catgagggca
     2881 gccgtaatac cgcctacagc acccgcacca agtacgagaa atgacagatc gtgacttttc
     2941 ataatctatg ttcatgaaat cataaccggg ccatgggcgt tacatcatgg ccgacatatt
     3001 ctcctctaag gtacttataa taaccggtaa ttccaatcat tgcggcattg tcggttgtat
     3061 agccgagcgg cggaacatag gtgttccatc ccctttttat gccttccctt gtcagggctt
     3121 cccgcagttc tgaatttgcc gatacgcctc ctgcaattcc tatttccctg atgcctgtct
     3181 gtaaggaagc cctgacaagc ttgccaatca atatatctac gatggtagcc tgcagtgaag
     3241 cgcaaaggtc gggaatattc ctgctgataa aatccggatc ctcttttgtc ctgtccctga
     3301 ggaaatagag aaaactggtt ttcaggccgc tgaaactgaa atccagacct tccacaaacg
     3361 gttttgcgaa cctgaatgca gtcctgttcc cctgttttgc ctgcctgtcg atggcaggtc
     3421 cggctggata tggcagtccc ataagcttcc cgcatttatc aaaagcctct ccggcagcat
     3481 cgtcgatggt atttccaatg atttccattt caagataatc cttaagtact ataagcatac
     3541 tatggccgcc cgaaacgagc aggcatagaa acggaaacga cggctgctgg tgtccggcct
     3601 gctcttccag gataaaatgg gcaagaacat gagcctgcag gtgattcacc tcaatgagtg
     3661 gtattccaag ggcaagtgca acccctttgg caaaagaggt tccgacaaga agggaaccga
     3721 gaagtccggg cccgcgcgta aatgccaccg cattaatatc cttcctgctg atcccggcct
     3781 gcctgatagc aacatccgtt acagggacaa tattggcctg atgagccctt gaagccagct
     3841 caggcacaac acccccgtac atcatgtgca cctcctggcc ggctataaga ttggacaata
     3901 tatagccatc cctgatcacg gcaacggagg tatcgtcgca ggatgattct attgccaata
     3961 tgatgactgc catctttact ttgaatttcc gtatatttta ggttattttg cctatgattt
     4021 ttaatacatt acattaatgc tcaaaagtat taaaaaatca tttaagtatc ttgtcatact
     4081 ggccggtatc attatgctgg tgccgacatc tacttacctc ctgctccaga tccctgaggt
     4141 ccagacatat attgttaaaa gaataacagc acatttttct gaccagataa aatcgacaat
     4201 cacggtcgga cgttttgaat acgctttctt caataagctc atcctcgaaa atgtcctcat
     4261 caaggacaag aaccttgaca cccttttata ctccggaaag gttaccgcag ggatccgtaa
     4321 acttgattta cgcaacagca atatcaggtt cggaagggtc aggttcgacc ggccccttgt
     4381 agcactcata accgatacca ccgggctgat gaatctgaac tggtatctcg acatgctggc
     4441 ctcctccgat acatcttcgg cagggaaaaa gagcagaata tcaatcgatc aggctgaact
     4501 aaacgatgcc cgcttcaacc tgatcgacag gagaagcagg ccgggaaaaa gcctgatcga
     4561 tttcgggaac cttcatgtcg gcaggatcag tgctgaagtc cggaatttta cggttaaaga
     4621 cgattcagtg aatttcagga tagccaggct gagcttcatt gaaaaaaacg gttttgccgt
     4681 ggaaaacttc gcaggcaggg taaccctggc agatcatgac atacttttca acgatatcca
     4741 aatcaaaaca gggaaaaccg atttaaaagc tgataacgtt tatcttactg cggactccgc
     4801 aggatcattc gcaaggttca acgaaagagt ccgcctggat attctgcttc gccggtcaat
     4861 tgtttcttca gctgatcttc agtattttct ccctttcctt gaaggtactg acgaaacagg
     4921 tgaagtttcg gggagagtaa ctggtttcgt ggaagaactg aagggaagga acataaatgt
     4981 ccggtacggc gccggaacac ttctcgattg tgattttgac ttttcggggc tctcatccat
     5041 ccaggatgcg tttattcatt tagggatcaa cacactcgta acaagcgcat ccgatattga
     5101 aaggatttcc ctgccgggaa agggaaaggg aaagatccgg ttgcctgaag taatgcacaa
     5161 gcttgggcaa ataacctttg acggaagttt cactggtttt gtttctgatt tcgttaccta
     5221 tggaaaaatc ggaacagaga aaggacatat caggaccgac ctttcagtaa gaccatggca
     5281 gggcagcagg ttcaaaataa aaggccttat tgcaggcagc gggattgagc ttggcgatct
     5341 gacagggaac agggatttgc tcggaaaaat gagtatggaa acctccattg acggctttgc
     5401 ctcttccatg gagaaaattg agggcgacct gaccggtgta attgacagca ttcagatcaa
     5461 taactactta taccggaatg tggccatcaa cggactgttc accgaaaaga catgggatgg
     5521 aagcatcagt atttctgaca ggaacatcag gatggatctt ctgggattgt ttgatttccg
     5581 aaatgccctt cctgagtttg attttacact gaatctcgcc gaatccaatc tttatgaact
     5641 gaacatcgac aaatcagatt caacttcggc gctttctatg cttgttactg ccaatttcag
     5701 gggaaataat attgacaatc tgtatggaga gatcaagctg ctcaattcaa cactgaacaa
     5761 gttcggaaac aaactggaac tctacaactt ctccctgaaa gcatttacag aagccaacag
     5821 gcctgcaata tcgctgagaa cggatttcat tgatgctgac ctcaggggct attataattt
     5881 cggcgacctg aacaatgtaa taaaatcagc gcttgcatcg ctgatgcccg cacggttccc
     5941 ggtaccctat gcgggaaagg tgcagataaa aaatgatttc aatttttcag tgaactttaa
     6001 aaataccgac aagatcaatg cctttttcag aacaggagta tttattgctg acaaaagcac
     6061 aattaccgga gaaatacatc ccgacaacaa cctccgcctg ggaataatgt caaaacagct
     6121 ggcgataaag aactattctt taagcaatct gacatttgat gcggattgcc aggaaggaaa
     6181 actaaatgcg gagctgaaaa gtaccgtatt gtcactcctg ggtgagactg agctgaaaga
     6241 ctttacatca accctcagca ccctgcctga taattttttg ttcaaccttc actgggacaa
     6301 caaggatcgt ataaaaaaca aaggcaatat cacggcagtt ggcaattttg ataaaaatga
     6361 aaatcaacgc cccgtgatgc atatcagcat agactcgtct gaactgtata cacgcgataa
     6421 tctttggaag atcaaacgtt ccgaaatctt gattgacaca acttctgtcg atttcaaccg
     6481 aataatgata gtgaacggaa ataattatta tctcgttgac ggaacaatct cggaggatcc
     6541 gacagatacc ctcaggctcg aattcgtggg aattgaactg gatcctctta atcacctcct
     6601 ctccggttca aatgacggaa aagagaatct cgattatggt ttcaggggaa cccttaacgg
     6661 gggtatcttt ctgaccaaca tattaaagaa cccgttactt gagggtgatc ttaaaatagc
     6721 cggtttttca gtgctgggaa gcgaatatgg agatctttcc cttgaggcaa catggaactc
     6781 tgaacgaaag gtggcggata tcagcgcctc aaataatctc aggggtgaaa aaaatctgga
     6841 tgtcaaaggg atatatgatc cgggggataa gctgtttaat ataagtgcga aggcttcaaa
     6901 gcttccggta gacgcactga atccgctgct ggattttttt gcctcggata tcaggggaac
     6961 tgcatcagga aaagttaatc tcaggggaga aaaaggcaac ctgtttctga caggagccct
     7021 tatggctgag aacacttcgc tgatggtaga ctacctgcgg accaggtaca ggttaaatga
     7081 caccataagg tttgacagga acggaatagt gttcaggaat ataaaactca cggatgagag
     7141 gggcaatctt gcaaccatat cgggatcagt taatcacaag tccttcaagg actttacggc
     7201 cgatctaatc atcaatatga acgactgcat ggtacttaac accctggcaa aagacaatga
     7261 cttgttttac ggtacagcct atgcccgggg ggttacaaca ataaaactca gacccgacct
     7321 gctatctttc gatatttctg cccggaccgg taaaaataca aggtttttca ttcctctgaa
     7381 ttccggaatg tctgtctctg aaagttcatt cataacgttt atggcatcag atacctcaag
     7441 gctgaatgac caccacaggc ccgaccggat attgccatcc gctgctgcgg gagcagatct
     7501 ggagctgaat atagatcttg acgttactcc tgaagctgaa gttcagcttc ttattgaccc
     7561 aaaggccggc gatgtcataa gggggcgcgg aacaggacag cttaatatca accttaaccg
     7621 aaaaaaggag ttcaggatat cgggtgacta tatcatcgag gaaggtgact atctttttac
     7681 gctcgggaat gtcctgaaca agagattcga cgtggagaac ggcgggaaaa tcacatttaa
     7741 cggggatgtg gaaaatgcag aaatagatct taaagccaag taccgtaacc tgaaaacttc
     7801 cctttatcct attcttggtg acgaacgtta caacgaaaga attcccgtcg agccccagct
     7861 taacctctcc ggaagactgt tcaatcccgt tgtaggtctg aacatttacc ttccgaatgc
     7921 tgatgaggag accagaagcc tgcttcagaa ttcaataacc actgaagaag aattaagccg
     7981 gcaattcctg tatctcctcg tcatgaacag tttttacaac gatccgtcaa tggggatatc
     8041 ctcggtcagc accacctcaa caggtacatc ggcaatggct gttactacca ccgaaatgct
     8101 gtcaaatcag cttagcaact ggctttccca gatttataat gattttgaca tcggttttgt
     8161 ttacagacca ggcaataagg atatcaattc acaggaactg caggttgccc tttcaaccca
     8221 gcttctcaac gacaggattg tggtgaacgg caactttgac atcaggggtg caaacaattc
     8281 atacggaaac ccgattacag gggattttga tatcgaatac aaaataaccg agaagatcag
     8341 attcagggct ttcaacaggt tcaataaccc gtataccgga aggggtgcgc catacacaca
     8401 gggcctggga ctcttctata aacaggattt caataaactg ttcaggccga aaacaaaaag
     8461 tcaaacgtcc gacatgaaaa aggaagacga aattgtcgta aggtaaaaga gttaataact
     8521 attcccataa tgttattaac catggataat agactgtatg gtttttccgt tttttaataa
     8581 cttcgtccgt gagaaaatat aagagtattt ttaatgtaat ttactgtttt aaaataaatt
     8641 aaataaagca cagagatgat ctttttacaa aatgtcatgc aggatgttcc tggcactgtc
     8701 cagatgatga gcgaaaccaa aatgaccctg ctggattttg caataaaggg aggctgggta
     8761 atgatcccga ttgttctgct ctcactggtt gctgcctata tcttttttga aagatattat
     8821 gtcataaaca gggcatccag ggaagacacg aatttcatga acaggataaa agactacatc
     8881 cacgacggca aggtggaatc ggctctcgcc ctttgcaaat caaccgaagg cccttcggca
     8941 agaatgatag aaaaagggat aagcaggctg ggaaggccgt tgcaggacat cagcaccgca
     9001 attgaaaatg tgggaaagct ggaaatttca aaactcgaaa gaggatttcc cacactggct
     9061 acaataaccg gggcagaacc catgctgggt ttcctgggaa ccgtaatcgg aatggtacag
     9121 tcgttttatg ctatgtcgca ggccggaaac aatattgaag tatctttgct ttcaaatggt
     9181 atatacaccg cgcttataac tacggttgca gggctcattg tcggcatact tggttatttc
     9241 gcatataaca cactggtggt cagggtagaa aaggttgttt tcaatctgga ggccaccttg
     9301 acggagttta tggatatcct taacgaacct cttaaaaccg gacgttgaaa tggcaatctc
     9361 ctcaagacac aagatcagca taaacttcag ctcaaccgga atgaccgacg tggtgttcaa
     9421 tcttctcatt ttctttatgc tgacatccac actcgttcat ccgaccgcgc tgaaactcct
     9481 tttaccaaaa ggcagtaccc agacttccgc caagccgcag acaactgttt caatcacagc
     9541 cgatcagaaa tactacgtgg aacagcaacc ggttaccctt gaagctcttg aaagtgtact
     9601 taaacagaaa ctgggcgcaa accctgaaac atatatttcg cttcatgctg ataaaaatgt
     9661 acctttcgag acagttgtag cagtattgaa tatagcacag gccaataatt acaaactaat
     9721 aattgcaacc acacccagat aacaggctca gaagcatccc ccacgaaaag aaaaaagggt
     9781 tcataggtac actgatcatt catgttgtgc ttttggtact ccttttggtg gttgccttca
     9841 tccccccgaa acctccggag cttgaggaag gcatactggt aaatttcggt actgatgaaa
     9901 ccgggatggg ccagattgaa ccttctgctc ccgcagccca gcccgagact acgcccccgc
     9961 cgcctgcaac cctgccaaaa gtgcaggctg atgagccgct cctcacacag aataacgaag
    10021 aggccccgga agtaaaaaaa gttgacccgg aagccgaaaa gaaaaggctg gaaaaaatcg
    10081 aagccgaccg caagctccgt gaacagatgg aagcagaaag aaaaaggagg gaacaggaag
    10141 aggcagaaag gaaaagaatt gaggctgaac aaaaaagaca ggctgaaata atgaataaga
    10201 cccgggaggc cctggcgggt tcaaaaaatg caggtaccat ggcaaaaagc gaaggtgacg
    10261 cgggaggaag cggtaaccag ggtgatccgg gaggcgaccc cggttcacgg aacaggggcc
    10321 agggaagcgg cctgggcgac aggggaattt ccttttttct cgaaggccgc ggtttccagt
    10381 cgctgccgtc acccagatat gattaccagg gagaaggaaa ggtggttgtc gaggtaagtg
    10441 tagacaggtc gggaaatgtc actcaggcga cgcccggagt caaaggctca acaacgcttg
    10501 acgaatacct tctaagggtt gccagggaag ccgccctgaa agcacgtttt gacccaaaac
    10561 ccgatgcccc ggtcacccag aagggaacaa taacctatat cttcaagctg aactgaaccc
    10621 tcttcctatc cggctgagta aaggggaaaa gacttcatca ttgatacaac cctgtctccc
    10681 acccttctga tcacagactc atcatcaata tttgacagta cctcgtcgat catttcaaca
    10741 atgattccca tgtgttcttc ctttaatccc ctggtggtaa tggccggggt tcccagcctt
    10801 atccccgagg tcaggaatgg agagcggctg tcgaaaggca ccatgttctt gttaacggta
    10861 atatgtgaca aaacaagcgt attctcggcc tttttccccg agagctcggg aaacctggtt
    10921 ctgaggtcaa taagcatcag gtgattatca gtgccgcctg acaccacctt gtatcccctg
    10981 ttcataaatg cttctgccat caccctggca ttcctttgta cccggacctg gtaatctttg
    11041 aattccggtt taagcgcctc tccaaaggca acagccttgg aggcaattac atgttcaagc
    11101 ggtccgccct ggatcccggg aaaaacagcc gaattaagaa ttgccgacat catcctgggc
    11161 tcgcctttgg gggttgtgag accccaagga tttacaaaat cacgtcccat caggataata
    11221 ccgccacgcg gacccctgag tgtcttatgg gtcgttgagg tgactatatg agcatactca
    11281 aggggattct tcagcagacc tgcagcaatc aggcctgccg gatgagccat gtcaaccatt
    11341 aatatggccc cggccgaatc agcaatcttt ctcatccttg cgtaatccca ttcccgcgaa
    11401 tacgccgaag ccccgcctat gatcatcttt ggcttttcct tcatggccag ctcttccatc
    11461 atatcatagt caacaaggcc tgtgtcttcc ctgaccgaat atgcgacagg tcggtaaagg
    11521 atgcccgatg aattcaccgg cgatccatgt gacaaatggc ctccatggct cagattcagt
    11581 cccagaaaag tgtcacccgg tttcatcact gcaagaaaaa cagccatatt tgcctgggca
    11641 ccggaatgag gctgaacatt tacatactct gcgttgaaca gctttttcag ccgtgcaatt
    11701 gccagcatct ctgtctgatc aacaatttca caaccaccat aatatcggtt gcccgggtaa
    11761 ccttccgcat atttgtttgt tagtacggat cccatggctt ccagtacctg gggacttaca
    11821 aaattctcgg aggcaatcag ttcaattcca ttgatctgac gctggcgttc cttttcaata
    11881 aggtcaaata cagtattatc tctttccatt tcctgagttt atagatttca catgataatg
    11941 aacaagctga aaattttgtc agattgccta ttagcctgtt ttttatcttt ataacaaaga
    12001 taatgttata gtgtttaaat ataataaaac attatcccgc atcatccgaa aattgtgcga
    12061 gaaggtccct gtatgccgag aaaatccttg atttggatat aaatcccaca taatagcctt
    12121 tatcgagcac cggaagattc catgctcctg tttcactgaa tgttttcatg acagtgtcca
    12181 tgccttcgga gatatcgata tatgacgggg gcatcgacat tatttctttt accgagactt
    12241 tgtcataaag cccggtgttg aacattattt ccctcacatc atccagcagc acaacacctt
    12301 caagaatatt gtagcggtcg acaacaggga acagatttct ttttgaccgg gcaatgactt
    12361 ttaccaggtc cccaagactg tcagagttcc tgactttaat cagatcccgt tcaatttcct
    12421 tcttccagtc catgattgtc tgaactgcct tatctttatc atgtgtgatg agatcgccgc
    12481 tttcagcaag ctgaacatga taaatggaat gcttttcaaa actccttgtg gttataaatg
    12541 caaccgtgga tgttattatc agagttatca gaattgaata cccgcctgtc atttccgcaa
    12601 tcaggaatat agctgtaagg ggcgcatgca tcacacctgc cattacaccg gccattcctg
    12661 caagtataaa cgtgttctct gagatatcaa cacctatata cttctcgaga atgccggcga
    12721 tgaaaaatcc gttcacgccg ccaatgaaca gcgtgggggc aaagatgcct ccaaccccgc
    12781 ctccgccatt ggttgaactg gtggccacaa ccttaagcat gaccatgcag aagaagaata
    12841 aaataaagac cagatagttg tctgagaatc tgtaaaggat actgttgcca aacacatccg
    12901 cggtttttcc ctgaagcaac agcctgatgg tgtcatatcc ttccccataa aatgccggga
    12961 agagataaat aagagcggcc agggtggttc cgcctgcaat cagcctaaca tagtcattcc
    13021 tgatcttttc gtatcttctt tcgagcagga gtgtcatctt agagaaaaag agggatacaa
    13081 atcccgaaac aacgcccagc agtatatacc acggtatata acggatcatg aatacatcag
    13141 tcagactgaa ggaaaacagg acctcgttgc ccatgaggaa atatgccaca gtggcggcag
    13201 taaccgatga gatcaggaga ggcactattg aagatatggt aagatcaagc atcagaacct
    13261 caagggtaaa aactataccg gctataggcg ccttaaatat gcctgaaatt gccccggctg
    13321 ctccgcaacc cagcataagg gtaatgttac ggtaattcag tttgaagtat tcacctattg
    13381 ccgagcctat tgctgaaccc gtaagtacga tgggagcctc tgagcccact gatccgcccg
    13441 atccgatggt gagagttgtc gcaacaagag atgtccacgt attatgcttt ttaatcctgc
    13501 tttttttctt ggaaatggca tataaaaccc tgcttacacc atgacctata ttatccctga
    13561 caatatattt cacaaaaagc atggtcagca gcataccgca aaccggaaat aaaagataca
    13621 gatgccctgt cgactgctgg gtaagtcctt cggttacaat cctgtttgta taatgtatgg
    13681 cattcttgag aaaagctgct gccagtgcgc taataagtcc cacaacaagg ctcagggaat
    13741 atatgatctt tacctggtcg atcctgaaca gttcaaggtt ctttaatttt tcgaaaaaat
    13801 tgccatgaaa cattataatg caaatataaa aactataaca gaccttgagt tttttagtta
    13861 agtttgtcat acaatttatt tcggaatcaa atccgatcct taaaaatgat cgacttcgtt
    13921 aagttcagcc tgaggaacgg cctgagaata cttgtgcatg aggacaggtc aacccctctt
    13981 tcagctctta atctgcttta tggagtcgga tcaagggatg aagatcctga aatgacagga
    14041 attgcccatc tccttgaaca tcttatgttc tgcggcactg aaaatgttcc tgtttttgac
    14101 cgtccgctgc aaatggccgg cggtgagaac aatgccttta caaacaatga tataacaaat
    14161 tactatctta ctgtcccggc tgataatatt gagaccggat tctggctcga gtcggacagg
    14221 atgaacggaa tcaatttttc ccgtgacgct ctcgatgtac agaaaaaagt ggtgattgag
    14281 gaattcaagc aacgctatct caatcaacca tacggtgact cacagctttt actccgcccg
    14341 ctggcctaca ccactcacca ttacaaatgg ccgacaatcg gtaaaagcat ttcccatatt
    14401 gagagtattg agcctgaaca tgcccgggac ttcttttacc ggcattacgc ccccggaaat
    14461 gcaatcctga ctgtatctgg aaatatttca cccgacaggg tatttaaact tgcagataag
    14521 tggttcggac ctctggaaaa gagaacaacc cgtccgcagg tcattcctgc agaacctttc
    14581 cagactgaag aaagaaatct gattgttgaa agagatgtgc ccgtcgatgc cctttacaaa
    14641 gcctggcata tctgcggcag gaaagaagat gatttcttta ccttcgatct tctttccgac
    14701 cttcttgcgg gaggtgaatc gggcagactg cattctaagc ttgtcaggga taaaaacctc
    14761 ttcagcgata tcaatgcgta catctcatcg gacattgacc cgggtctgct ggtcattcat
    14821 ggaaaactga tgaaaggtgt gaccattacc gaagctgaag aaaatgtaag aatggttgtt
    14881 gatgagctta aaagcggcat tatttcggat gaggaaatgg aaaaggtcaa aaacaggttt
    14941 gagtcgtcac atgtgatttc caatacatcc atcctcaaca aagccatgaa catatcatat
    15001 tatgagttac ttggcaatcc cgggcttatt aaccttgaag ttgaaagata cagggccgtc
    15061 agccgggaaa tggttaggga tgccgcaatc aaatatctga acaataacaa ttccagcacg
    15121 cttcattacc tttctaaaaa caggaactga gaatgtcagc agaaaaaatg acggcaccga
    15181 gcatcaatcc catcaatccc gggtcacttg cacctgtgaa aagcattgtt ctgggaaacg
    15241 ggataccggt ccatatcatt gaaggtggaa ccgaagatgt aatgaggatt gaatttattc
    15301 tgagggcggg aaccgtgatg gaaaatattc ctcttcaggc ttcatcaacc aacatgatgc
    15361 tgaccgaagg ttctgaaaaa tactcttccg aagaaataaa ggccctcctc gacggttatg
    15421 gcgtatttta ccagctgtct gctgaaaggg attcagccgg cctgatcatt tatctcctta
    15481 acaggcatgc ggagaaagcc attgaactga tcgcagagat tttattcaat tccatgttcc
    15541 ctgaaaagga atttgtgata atgatgaaaa aacgcctcag gcggttcatg atcagccgcg
    15601 aaaaggtaca aacactggct tcggaaaaat tctttgaaac catttttgga aataatcatc
    15661 cttacggaaa aatggtatca gaaaatgact ttgaagcttt aaatactgat catctgaaga
    15721 atttctacgc cagttactat acgccggcaa atctttcagt aattgtttca ggtaaaatca
    15781 atgaaaacat acaggagatt atggataagt acctgggtag taaactcccg ggaacgccgc
    15841 tgcaagcggg aaatgaatca gattttcaga aatgcccaca aaaaaggatc catgttccaa
    15901 aaaagggggc attgcaatca gccatacgga ttggttcagc cacaataaac aagcgccatc
    15961 ccgattatcc cggactaaag gtgcttaata ccattctggg aggctatttc ggttcccggt
    16021 tgatgaaaaa catcagggaa gagaaaggat atacctacgg gatccgctca gtggcagctt
    16081 cacttaacct gagtggttat aaaatcatat cggcagaagt agataaccag tctgtccggg
    16141 aagctattgc cgagatctac agggaaatca gtaaacttca gaaagaaccg gtagcttatg
    16201 acgaactgga actggtcagg aattatatgg caggtgagat ggtccggatg tttgacggtc
    16261 cgttttcaat ggcagaaagc ttcagggcaa tatgggaatt caatctcgag gccgactatt
    16321 acagaaaact tgcccatact gtcaggacaa taacgcccga cgaactcctg cacctggcaa
    16381 ataaatacta taatattgat gaattatatg aaatcgttgc cggataacag acttagtaca
    16441 gtaacaggcc tcatgatact gctgatgtcg tcagtcctgt tcactgtcaa ctgtcaggag
    16501 gatactggtc cccaaccccc ggtattcagc cttgtttcaa tcaaccagac gacaggaaat
    16561 accgaaatgt cgtggactcc cagcctttct cccggggtaa ggggatacat tatatatctc
    16621 tatcagaatg gcgacggatt taagattgac agtatattca atcctttgca gacaacttat
    16681 tcagtttacc gtgcctggtc atccgttaga agcgagtcat ttggaatagc agcaattgcc
    16741 gacaccctga gccctatttc aaatatttta aagacgatct tttgtgaagc tgcagccgac
    16801 tcgtgcaaaa aaaacataaa ggtcacatgg aacaaataca tctcatttcc gacagctgta
    16861 acgggatacg atgtgctgag atccgtgaac ggaggcccgt ttagtattgc cggtcaaaca
    16921 caaaattccg ataccatctt tgtaactgat gattacgaaa acggcactga atactgtttt
    16981 gcagtaaggg caaggctttc agacggaaag tcatcatatt caaacaaagc atgtgtgact
    17041 gtacggataa cacgcggccc ggactggatc aatgcagatt atgcaacagt cacagagtca
    17101 gatgtcattt cactttcttt taccatcgat ccctcctctg aaacggatct tttcaccctt
    17161 gagcgcaaga caggcccttc aggttcattc aaagaaatcg catcgataag aacttctgtc
    17221 aatacggtta catatattga cgacgacgga aatcccaacc acaataacta ctacaggcta
    17281 tcagcaatta acagctgcaa tctgccggat aaaacatcaa atattgcatc gaatattctt
    17341 ttaaaatatg aacgcggtcc tgatgaaaac atcatattga aatggaacag ctactataag
    17401 tggaacggcg ttatcggttc gtacattctg tatatggacc agggcagcgg attctttcag
    17461 cattccttat taagtccttc tgacacaacc tgtttaataa gcattcctga agttatgttt
    17521 ggagcttatg gagagaagat atgcttctat attgttgccg gagaaactgc aaatccttat
    17581 ggcataaacg gggaaagcag atcaaacaca acctgtttca aactggaaga aacaataacc
    17641 gtacccaata ttttcacccc taacggggac gccagaaatg accttttcgc cccggtactc
    17701 acattcaccc cattggaata ccaccttgta atttccgacc gacatggcag ggttattttt
    17761 gaaacaaggg attttaccga atcctgggac ggatcagacc gaaacaatcc acccagggaa
    17821 ggtgtttata tctggtcgct cagagtcctt acaaataacg ggaataagat ctcaaaaaca
    17881 ggtacggtaa ccatctataa aaacaaataa tattttcgtg cagccattca aggggcatgc
    17941 tggcagattt cacttaaaaa gctatctttg tagttaccac aaaaggaagt gagagaatct
    18001 ttatggggaa taaggatatt ttcgatgagg aagatattaa acggattcag gatgaatatg
    18061 attctctgat aaataatctt tcaaagtgca ataaacccga ggacagggag cttatagaaa
    18121 aggcattcca agttgccaac aaagctcact ggaacatgcg gaggaaatcc ggtgaacctt
    18181 acattctcca tcccatagct gttgcaaaaa ttgtcaatca ggaaatgggg ctcggaaccc
    18241 gatcaattgt cacatcgtta ttgcatgacg tagttgagga caccgaatat tcgatcgaag
    18301 atatcagcag ggaatttggt cctaaaatag cctccctgat cgatggtctt acaaagattt
    18361 ccggaaccta taacaagggt tcatcatcat caatgcaggc agagaatttc agaaagatgc
    18421 tgatgaccct ttctgatgac atcagggtga tcctcatcaa gatagctgac cggcttcaca
    18481 acatgaggac gctggattcc atgccggaaa acaagaaact gaaaattgcc ggggaaacaa
    18541 tattccttta tgcgcccctg gcaaacaggc tgggattgta tgccatcaag agtgaacttg
    18601 aggatctgag ttttaaattc cggcaacccc aggtctatga agaaatagca acaaagctca
    18661 aacatggcga acagaaatac ctcgccctga taaacaagtt cagccttccc attattgaca
    18721 agctaaccga atcaggcctg cagtttgaga tcagcggacg cccaaaatcc atattttcca
    18781 tctggaagaa aatgcaggca aaaaatgttc cgttcgaaga aatatacgat gtcctggcag
    18841 tcagagtggt ttttgagcct ataccgggta tcccggagcg tacaacctgc tggagcattt
    18901 actcagccat tacggatgtt tatcttccaa aacctgacag attgcgtgac tggataagca
    18961 ggcccaaacc caatggttat gaagccctgc acgttaccgt tatgggccct gaaggcagat
    19021 gggtagaggt tcagatcaga agtcgaagaa tggatgaaat tgctgaaaga ggatatgccg
    19081 cccactacaa atataaaggg gataacagcc cggaaaccga actggacaaa tggataagga
    19141 agatcagaag catgcttgaa aatcccctgg aggatccgat tgagtttctt gatgaattca
    19201 agatgaacct gttctcatca gagatcatgg tcttcactcc caagggcgca cttgtaagcc
    19261 ttcccaaagg tgcctctgcc ctggatttcg cctatgaaat ccatacagaa ataggcaata
    19321 aggcgatagg cgccaaaatt aactataagc tcaatccgat caatgccatc ctgatgagtg
    19381 gtgaccaggt ggaaatcatt acttcagaca ttgcaaaacc tgaaaaggaa tggctgtcat
    19441 tcgtaaggac ctcaaaggcc aaggaggcca ttaagaatca gctgcgaact gaaaccacaa
    19501 acaggataca aaaagggaaa aatattcttg cgtcaaaact ttcagaactg ggagttgtac
    19561 ccaattccga aatagtaaga aaacttatgc tcagttatga cctgtttaac aaggacgaac
    19621 tatattcggg tattgagctg ggtaccatca gtctcgataa tctgaaaaag ataataagga
    19681 aaactccggc taaaaatgtt ataaaatact gggaattgaa gctgctgggc tcgatgaagg
    19741 ggaaaaaaga agaagaacat gaacatcctg aaaagattga tgacggtgct ccattcctgc
    19801 tcagggaaaa tgttgaagac gccggtcaat cctacgaaat agccaaatgc tgcaatccca
    19861 tacccggaga tgaagtgatc ggataccggt ctcccaaggg agcgatcatc attcataagc
    19921 ccaaatgccc cgtggcaatc aggttaatgg caaacgaagg aaacaggatc attgcagtga
    19981 aatgggcaat acataaactt gtatcattcc tggccaggat aagcatgacc ggagttgacc
    20041 ggattggttt tgttaacgat ataacaaata ttatatcgtc ggagctgaaa gtcaatatga
    20101 ccaatataaa cataagtgtg aaggacggga tcctggaagg aacaattgat ttgtatgttc
    20161 ataacaccca ggacatcaac aatctgatac tgaaaatctc caatgtcagg ggaattgtca
    20221 gcatcaagag ggttgaagat ttttcagaac aggatgcaaa ctgatccgta tggaagatta
    20281 tttgattttt aagattattt aaagcagtcg ggaaatggca gatgacgaaa caaaatccac
    20341 tgtcaaacaa atatttaccg aatatctcga gaagaaggga cacaggaaaa ccccggaaag
    20401 atatgccatt cttgatgaga tatattcaca gtcgggacat tttgatatag agtcactcta
    20461 tgttttcatg aaaaacaaga attacagggt cagccgtgca acactataca acaccatcga
    20521 attattgctc gactgcaatc ttgttatcag gcataaattc ggaagaaacc ttgcccagtt
    20581 cgagagagca tatcaatgca gccagcacga tcaccttatt gatgtggaaa cgggcaaggt
    20641 agttgaattc tgtgatccac gaatccggaa tataatcaaa actgccagca cccttcataa
    20701 ttttgagccg gcttatcact cactgtatat ctacggatcc ttcaaagaaa agaataaatg
    20761 atcttatccg gatctgcaat accagtatac cggaaatgac aacatttccg ggtcaaaatg
    20821 attatccgga gataatcagt ggctaaaatg cataaccgaa agtgaaaccg aaggggacat
    20881 tcactcctat cctgttttta aacacatcct catcccagtc cgacccctgc aaatcccagt
    20941 tccttgaaac aaatcccagg ccggtgtaaa catccagggc gaagcctcct gaagtaatga
    21001 gctgataacc gaatattccg ttcagattca actttgatgc cttaattttt tcgacatcat
    21061 tctgcttgtt ctttatctca acactggcat atgatgcatg agggcctatg tagaatcctt
    21121 ccggtgcaga aagatcacgg ctgagaaagt atttaaacat tccctggacc ctgaaaccgc
    21181 ccgtttttat gccttgtatc ccttcgcttt catcctggct tgtgatttta tcgagattaa
    21241 gaagaacact cggcccgata tatccggcac caacctggat agacgttttt tcagaaacct
    21301 ttgcttcaaa aaacgctttg tattcaccgg taagcgggac tactattacc cagaatggtc
    21361 cgccaagagc tgacagggga ttggttttga tcgtgaacct gaaatccttt tctccttcgg
    21421 atttctgagc tgatagatta tttgccgtga acagcagaat cagcacaggg ataagaaaca
    21481 cttttaaatc tttcattttg aacgcaatta aattaaaaat aaatttttct ataaaaatac
    21541 tttatttcaa tcaattacat cagataacgt aaacctgtta ccgacaattg ttccgtttta
    21601 tcaacaatat cagattcaac gctttctcac gagcaaatat tttgtacttt cggaattgtt
    21661 tcatattttt ctgtttaaac ctgaacaatg aagatcgata tcttgctcgg attacaatgg
    21721 ggcgatgagg gtaagggcaa aattgtagat gcccttagtc ctggctatga tgtgattgcc
    21781 cggttccagg gcggaccaaa tgcaggtcat tcactggaaa tcaataatgt taaacatgtt
    21841 ctgcacctga taccctctgg tatttttcat ccaggcaaac ttaacatcat aggcaacgga
    21901 gttgtgcttg atcctgccgt attcaggcag gagatagaac accttggcat accgcttgat
    21961 gaactgagaa aaagactgat aatatcctca aggacacacc ttattctgcc aacccacagg
    22021 atcctggatg cagcttatga aaacaggaaa ggcaaccata agattggatc cacccttaaa
    22081 ggcattggac cagcctacac ggataaagca agccggaacg gtataaggac gggcgatttg
    22141 cttagggagg actttacccg cctgtatgag gaacatgtaa actgtcacat tgaattattg
    22201 aaaaacctgg atcacagttt tgacctgaaa gcctatgaac aggggtggtt cgaaggcatt
    22261 gaacttttaa gatcattcag aattgacaat actgaatacc ttattaacga actcgcatca
    22321 aaggggaaga aaattcttgc cgagggtgca cagggaacga tgctagatct tgacttcgga
    22381 tcatatccgt ttgtaacatc ttcaaataca acgagcggag gtgcatgtac cggcctgggc
    22441 atatctccgc gtctcacggg cgaggtattt ggaattttca aagcctattg taccagagtt
    22501 ggcagcggac cgtttccaac tgaattgtcc gactcaacag gtgaaagatt aagggaaagg
    22561 ggacatgaat acggagcaac gaccgggaga cccagaagat gcggatggct tgatcttccg
    22621 gcacttaagt atgccattat gataaatggc gtaacgaggc ttttcatgac aaaatccgac
    22681 gtcctttccg gctttggaac cataaaggtc tgcagatcat acatcagagg caacaaggaa
    22741 agcatggagg tccttgccga cagtaatatt gaaaccgggc tgatttacga ggaatttgac
    22801 ggatggagcg aagatatctc ccggatcagg gaattcagtc agctgccggc cagcctcagg
    22861 cgctatattg aatatattga acaggaaaca ggcatacctg tatcacttgt ttctgttggt
    22921 cccggcagaa atgaaaccat tttcagataa acgaaccaga cacatctaaa caactaaaaa
    22981 tcagatatcg gatgaaatca ttaatccagt tttttgaaga aagtgttgac aaattcaatg
    23041 tcaatgtcta cctatgggaa aaaccggttg acagatatga aggtacaact tacggcgaag
    23101 ccagaaaaca ggtttacgag tttgcagcgg ggctgcttaa acttggcata aaaaaaggag
    23161 acagactgag tctgatatct gagggaagga acagctgggt aatcggagaa ctgggaatac
    23221 tttataccgg ggcggttaat gtaccattat cagtgaaact aagccctgag gaaataaagt
    23281 tcaggattaa ccattccgga tcaagaatga tcctgacgtc ttcaatacaa tcacaaaagg
    23341 tcaaacagat cataaacgaa tgtcccagcc tggaaaaaat aatacatttt gatgccctgg
    23401 aagaatatga agaaaatgag atccatttca atgaggtaag gaagcttgga agggaatggc
    23461 tggaatctcc tgagaacaaa tcgctctttg atgagatcag taaaaacatc aaaccatcgg
    23521 attttgccaa tatttgctac acctcaggaa ctaccgctga tcccaaaggc attatactta
    23581 cccacggcaa ctatgtcacc aatgtttatc aggcctacag cctgatggat atcccctcgt
    23641 tctacaagac tcttctgatt ttgccatggg atcattcctt cgggcatacc tgcggaattt
    23701 ttgccttcat gggaaaaggg gccagcattg cttcagtcaa aacgggcaaa acgccccttg
    23761 aaacgctcag gaacctgcca tcctgcctca gggaaattaa gcccaatctg ctgatgagtg
    23821 ttccggccat tgcaaagaat ttcaggaaga atattgaaaa aggcattaag gaaaagggaa
    23881 ggctggcaga aatgctcttc aaccatgcac tcaggatctc ctacaagtac aataaggaag
    23941 gctggaacaa ggcgaaagga ctgcagaagc ttaaaaaacc gctgattaat ctctatgata
    24001 aaatattgtt cagcaaaatt cgcgaaagtt tcggaggcga gcttgattat tttatcggtg
    24061 gcggtgccct cctggatatt gagctccaga ggttcttcta tgctatcgga atacccatgt
    24121 accagggcta tggtttgagt gaagcttcac ccgtaatctc atcaaattcc actgcacggc
    24181 acaggcttgg atcatcagga gtgctcgtga acaacatgga tcttaagatc tgtgataatg
    24241 atggtaagga agttcccctt ggagaaaagg gagaaatagt aatcaggggc ggcaacgtta
    24301 tgcacggcta ctggaaaaat gagacagcca ccagggaaac catcagggac ggatggctgt
    24361 atacaggaga tatgggatat atgacaaagg aaggatacct gtatgttctc ggcagattta
    24421 aaagtctact tattgccgac gacggtgaaa aattcagtcc tgaaagcatc gaagaagcgc
    24481 tgactgaaca gtcaaaatac attgatcagt gtatgctgta caataatcag aaaccatata
    24541 cggtagccct tatcgtcccg aatcagcatg ccctcaaggt ttaccttgaa ggaagggatc
    24601 tgacggctga tactgatgaa ggaaaaaggg cagtgctgac actcatcgaa gatgaagttg
    24661 acgaataccg cactcatgga aaatacggca atatgttccc acagagatgg cttcctgttt
    24721 cccttggaat ccttgaagag gggtttactg aagaaaacgg tctgatgaac tcaaccatga
    24781 aaatagtcag aggaaaaata atggaccgtt accaggagct gatcgaatat ctttatacgc
    24841 ccgatgccaa aattataacc aacgaaagaa acatagaaga ggtggaaaaa atgaaactgg
    24901 gttaatgggg aaaatcagca cgggatatgg gattgtttcc tgcagcacct ctctccttat
    24961 gtggtcctac cctgaatgaa gatatatgga acccggagca gttgaaccgg aacataattc
    25021 atgagaccaa ccggttagcg gcttagagga ttttagtcat gaataccggt ctctgatttt
    25081 ggaaatgtgt ttcccgtaac ccgttaaatg ggatcacaag ctgataaaac aacaccggaa
    25141 accggaacgt tgccggtatc gcgtagggac gctaatatag tcagagctgt ttgaccggat
    25201 ttgtcgtatg cttttctcca atcctgctaa tccagagcat aaaactcctc ttttccggcc
    25261 ccgcagaccg gacaaacata ttcgtcagga agatcgctga atgcaattcc cggcctgata
    25321 tccgaaatgc agtcaccctc ttcgggatca taaacatatc cgcaaaccga acatttatat
    25381 ttttccatat atgatattaa agtttgtgtt tcccattgat cataataccg ccggttttct
    25441 acgttatttg ttcctacaaa agacctgaca tccggtaaca ttcatgtctc ttttttcccg
    25501 gaagcaggct ggaatctgtg cgctttccgg catatcgata tttttacctg ttttccctgt
    25561 tttcacaaaa ctcgatcaga acgccggaag cggatgcagg atgcaggaag gcaatgttca
    25621 aaccgtccgc tcccggacgg ggttcttcat caataagcct tattcccgca ttttttgttg
    25681 cactcagttt atccttaagg ctgtttacct taaaagcgat atggtgcagg ccttcgcccc
    25741 gcttttcaat aaacctgctg atcggacctt ccggatcagt tgattccagc aattcaattt
    25801 tagttgtccc gatcttaaaa aaggcagttc taactttctg gtcacttact tcctctattt
    25861 tctgacattt cagcccaagg atggcttcat aaagccggat tgattcctca aggctcctga
    25921 cagctattcc aatgtgttca atattggttg gtttcattta tgtctgtatt tatttgcgcc
    25981 agcattccgt aatttcacct tccccgattg ccataaaagg catcattcat gaccagatct
    26041 gatctgttca tctgcctccg gtaccagtca gaacgggaaa tcatcgggga cactcacgcc
    26101 gggctttcca atatccggtt tttttgatgc ctggtgaaca ggatttccat tcacatagat
    26161 tgccttgtat ggaacggtag ggcatgaaaa ctcgcaatgt ccgcatccaa tgcatatttc
    26221 ctgattggta tccgggataa cgaggcttcc ttcgtaagga atcatgaaaa ctgcctttgt
    26281 cgggcaagct tcggaacaag ccccgcacgc agttttttcc gttttaacaa tgcaattatc
    26341 cttgatgaat ataacctttc cgatctgtgt aagctttttt gcctcaggaa gcaatggctt
    26401 cagggcaaat gtgggacata tttcagtgca cagtgtgcaa ttatactggc agaaaccctt
    26461 atggtaatcc attacaggct gcatataacc ggcaagacca tattgcatta ttgcaggttg
    26521 aagaacatca ttgggacaaa cggaaataca caggccgcat gccgtgcaac gattgttgaa
    26581 atcactgatg gatcctgcgc cgggaggact gaccggacaa ttcttttctt ccttcaccgt
    26641 cgactctctc ctgggaaccg ggatccccct ttcctgggcc cggcctgaac ctgaaagacc
    26701 caaaaacagc attatggatc cggcaatgaa ccggcgcctg ctttcatcac tcttcttttc
    26761 ctcgcgggta tttccgataa agccgtagga aagcgcttta tcggggcagg aatatataca
    26821 gtcaaaacaa ttcacacaac gtgaaacgtc aatctcatgg ttcagaaaat caatgcacga
    26881 tgatttgcat acaacagaac acctgccgca gcgtgtacat ttgttttcat cagcttttat
    26941 cctgaaaagt gaaagcctgg aaacaagacc cagaaaagta ccaaccggac agatcatgtt
    27001 gcaataaagc ctgccctttg tcaatgacaa caatcctgtg agaataaaaa agcccgcggg
    27061 aatggcatat gccataaggt tatatccttt gatttccaaa tgatgcagcg cataattgtc
    27121 gaatctcccc aggattcccg caagaaaatt attcaacacc aggataacgg gtttgataaa
    27181 aaaaagaatt gacctgccga atatgctgta cggatcaaga agattgataa aatacattcc
    27241 ccaaataaac gtaccggcaa taaccaacac caggactgaa tatcgtacaa ccgtatgcgg
    27301 ccgtttgtac ccgaatcttc tgaatttctt tcttatggta ccccctatcc tgctgaatac
    27361 atcctgacca attccgaggg ggcataggaa cgagcagtag gtcctgcccg taaccagtgt
    27421 cagtaaaagg actatcagaa atccgccggc agcaagtgac tttatatcaa tgaatttaat
    27481 aatggatgga ataaactgca gatataaaag atacttgata tattccggct ggatcaaggt
    27541 tctgaattca gcgaaaatat aaatgaagca gaccagaaca actgcagaga atacaatacg
    27601 taatcgtctg agaaaggccg aggtcattta aaactacatt tttatacgtt tgatattcag
    27661 ggattccagt ttaatgttcc ctattcccat cttgtgtgca atcagaatat aatctatctt
    27721 ttcaggtgca attccaagca tccttgctgc agctgcatct ccggcaaccc agtcagtggt
    27781 aaggatctgt gatttcatct gcaccacatc ttctttcgaa acaccctgcg ggccatgctt
    27841 tgcaattaca ttatagcaat caattacatt gagggccggt ttttttttga acaaggcata
    27901 atcggcaata cactgatgga gattgttggc gtgccagtat cccctgttcc agacaatgcc
    27961 catggcattt ttcatggcac aggtgatcct tgttgagcta tgatccttca gaacaggcac
    28021 gttgatgaaa acatccgttt caagcagcaa ttcatgtacc ttggcgtttt ttagctttac
    28081 ggcggtggga attgtaattt catgataata tttttcagaa tttgccggag ccaccttggc
    28141 acccgccatc ttagcggctt tctcaatgcc ggagttcctg taggtattga tccagttatc
    28201 tatggtatga tcaaaaatat acacttccct ggcaccggcc ttaagacaat gttcaatgat
    28261 gcgctttacc aggagcggat tggtatttgc agcaagatcg ggagttacat cccagccaat
    28321 attcggttta atgagtaccc tctgcccctt gctgacaaaa ttacccatgc caccaagctc
    28381 ctggattccg agatcgaaca tttcatccgg atcacctccc atcactgcaa ccagatcgta
    28441 tttcgctcct gtaccgttgg aagcccatac cctgttgtat cttccaagac tcaaggcagc
    28501 cccggctgca attcccgcac ccagagatgt tttaaaaaaa cctcttcttt tcatttttta
    28561 agaatttgca ttgcaatccc gaaaaggttt cgaaaaatac tacttttttc ttataattcc
    28621 ataatgaatg taaattgtat cttagccata cggttgattt attacttaga tttgccgtaa
    28681 cattaatccg gattaattgt tacacttaat attatatttt atgaaggttt tatgttatga
    28741 ttgtttttca gggatcagcg gcgacatgaa tcttggcgca atgcttgctc tgggaattga
    28801 ggaatcttat ctgatcggcg agcttaaaaa gctgaatctt gacggatggg aacttctggt
    28861 ggaaaaggat cagcgtcatg gaattgcagg gataaaagct acagtcaggc agaaaggtca
    28921 tgatcacagc cacaggcatc tctctgatat tgaaaaaatc atatcagatt ccgggctcgg
    28981 ggaagatgtt aaaaaactca gcatgcgcat tttcatgaag gtggctgaag ctgaggccca
    29041 tgttcacggt atcccggttg acagggttca ttttcatgaa gtgggcgcaa tagactcaat
    29101 tatcgacatt gcaggtgcgg ctatttgctt cagtgcactg aaggttgatg ccgtatatgt
    29161 gtcggaagtt gaacttggag gaggatttgt cgtatgcgaa cacggaacgc tgccggtgcc
    29221 agcccctgca acagccgaaa taattaaagg cattcctgta agaaaaggag ggcttgattt
    29281 tgaagccaca acacccaccg gcgccgcaat acttgcggcc cttggtactg atttcagtaa
    29341 aacccattcc ctgaaaatcc ttaaaacagc atacggaatc ggacaaaaag aacacaatga
    29401 tgttccgaac ctcctgaggg tgtctttggg tgagacaact gattctgaaa ataccgggca
    29461 taatgccgtc catctcgaat gcaatatcga cgacatgaat cctgaatttt atgactacat
    29521 ctccgaaaag ctcttcagtg ccggagcttc cgatgttttc tttacgaata tcataatgaa
    29581 gaagggcaga ccggggataa tactcaacgt tatttgtgaa acagggcatg aagacaatct
    29641 gaaggagctg attttcaaag aatcaacaac cgttggcatc agaaccgttc aggtaaggaa
    29701 agacaccctg gtaagagaat tcacaaggct taatacaaaa ttcggtgaag ttactgtcaa
    29761 acattcgtac ctgaaaggat ctgaagtttc ggttaaaccg gaatttgatg atgtcagaag
    29821 gattgcagcc gaaaacggga tcccggtcaa ggatgtttac agtatgatat tatcgatgat
    29881 aagcgccaac aggaaataat ttatgtttaa caatctgctc gataagcttg atcagatact
    29941 taccgaactg gagtctttcg tcgttgcata ttccggcgga gtcgacagct ctttcctcct
    30001 ttacagggcc cattcaatca aaaaagcgga tgttattggc gtaacaatcc gaactccgta
    30061 tattccggac tatgaaataa atgatgcagt tgaatttgta aagattcacg gcatcaatca
    30121 cagaataatc gatctttcat ttccggaaat tatccggaat aatcctgtag aaagatgtta
    30181 ttcctgtaaa aagaccctgt tttcggcgat gatcaccttt gcggccgaaa acggcttcag
    30241 gtatgttgtc gatggttcga atgcagatga cattaatgat ttcaggccag gcctgaaagc
    30301 cctcagggaa ttgaatatca gaagtcctct tctcgaagcc gggttgacaa aaaaagatat
    30361 cagggaaata gccttcaaac acaagcttcc tatctgggat aaacccgcaa tggcctgcct
    30421 gcttaccaga attccctata atacagaagt gaataccgag accctgagga tgattgaaag
    30481 agctgagtcg ttcctgtttg aaaacggtta ccccggtgca agagtgaggg ttcatggcga
    30541 tcttgcacgt atagaatgcc tcccgggttt ttttgaaagg attattcgtt cacccgaaaa
    30601 agaacttata acggaaaatc tgaagagaat cgggttcaga tatatatctt tagatcttga
    30661 aggctacaga tcaggcagtt caaatcctga aaaataaaca tatgacgaga aaagaaattg
    30721 aaatacttct cagggaagtc agcaagggtg aaaaaagcgt tgatcaggca atggagatat
    30781 taagggattt tccatacact gacctgggtt atgccaggat cgaccaccag agggaaatgc
    30841 ggaccgggta tcctgaaatt gtatactgcg ctggaaaaac cattgaacag gtagccggga
    30901 tcttcagggt catgtcggaa aaggaaaata atgttgtagg aacccgggct aacccggaaa
    30961 tgtatgaagc tgtcaggctg ctgattcctt ctgcgatata ttatccggaa gccagaatta
    31021 tcagtgttca gaaaaagaaa cctgtgtgtc ccgaaacccg gattgctgtc atcacggcag
    31081 gtacctcgga tatcccggtg gcagaagaag cagccgtaac cgctgaactg ctcggcaata
    31141 atgtgttcag gacctatgat gcaggtgttg ctgg
//
LOCUS       DAOOYG010000020        30938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1252_length_30938_cov_7.294110, whole genome shotgun sequence.
ACCESSION   DAOOYG010000020 DAOOYG010000000
VERSION     DAOOYG010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 30938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30938
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1252_length_30938_cov_7.294110"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK288_03315"
     CDS             <1..214
                     /locus_tag="PK288_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPM17979.1"
                     /translation="SNRNKLMTREDILTEIWGEDDYFLGRSMDVFISRLRKYISGDRN
                     IDIRTIRGTGFILEEIRPGEPDGERF"
     gene            complement(224..1330)
                     /locus_tag="PK288_03320"
     CDS             complement(224..1330)
                     /locus_tag="PK288_03320"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPM17980.1"
                     /translation="MKKAYAFLISAIIVASCSSGPQYKITGKIEGADNVTFLLQKRAD
                     GKLVTIDSAVSKKGNFKMQGGPVKYPEMVLLTVKDSRMRTTFYLENSNIQIRGRLDSL
                     FNAKITGSKTQDEYRRFVDSNKALNDQYSRAYNDYQSAFQENDTARLRILENEILSLQ
                     ESMINLQKDFVKNNPSSFVSPSILAGLSREMEAAELETLINAFDTALKNTPAVKELTE
                     LVAMMKPVSIGQKAPDFTLNDPRGNPVSLSSKIGSRLLLVDFWAGWCGPCRRENPNVV
                     RIYNEFHRRGFDILGVSLDRDREEWLKAIADDKLTWTHVSDLQYWNNAAARLYVVNSI
                     PANFLLDDNGIIIARNLTGNDLYNKVKEILDSRQ"
     gene            complement(1383..2480)
                     /gene="meaB"
                     /locus_tag="PK288_03325"
     CDS             complement(1383..2480)
                     /gene="meaB"
                     /locus_tag="PK288_03325"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPM17981.1"
                     /translation="MKNKEDFKNAAKVQPAVNYPPEVNPVAFSPSGKKKKRKYSADEY
                     VEGILSGNRTILSQAITFIESTLPDNAEIARQIIDKCFPLSSRSVRIGITGAPGVGKS
                     NFIESFGLHLTRLGRKIAVLAIDPSSAQTKGSILGDKTRMEKLSIQENAYIRPSPSAG
                     TLGGVADRTRETIILCEAAGFDTIIVETVGVGQSETAVRTMVDFFLLLLSASAGDELQ
                     GIKRGIMEMADGIAVTKADGPNRLNAERARISFQNAMNLFPLPTSGWKPAVMTCSAHQ
                     NSGIAELWDNILEYVRHTTSGGYFEKLRKEQEVTLMHAIISESLTSSFYSDKKIKPLL
                     KSLEKQLYEGKITSHNAAFALLDKYHIKRAP"
     gene            complement(2572..3666)
                     /locus_tag="PK288_03330"
     CDS             complement(2572..3666)
                     /locus_tag="PK288_03330"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPM17982.1"
                     /translation="MKKLFLFFALAFCGLLSWGQPVMKISGTEHDFGKFREEAGRQKY
                     DFVVMNTGNQPLVIQNIVASCGCTLPEWTKTPIPPKGTGTITAIYDPANRPGPFNKTL
                     SVYSNSKPEVVVILLKGEVIPREKTLEEIFTFPVGSVRFESNHLAFTNVKKTEKKIRV
                     MQLINTSNSPVKVEFEGVPDYLSLKVKPETLKAGEKGIVEGTYDATKNQTWGNVSSMV
                     RVKTNGVLHNNVYYYVSANLVEDFSGLSKEELANAPVFKPEKTTIDLGKIAGSTQNEV
                     EYKFTNLGKRDLVIRHIRSSCGCTAVQQGIQGQGIKPGESGSIKATFNSGGYKGKVTK
                     AIYVYTNDPKNSEVMLMVTADVAQEVSASK"
     gene            4082..7357
                     /locus_tag="PK288_03335"
     CDS             4082..7357
                     /locus_tag="PK288_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17983.1"
                     /translation="MKNTRRLIFILAAMLLFFRAEGQFYNGLQMPFGKNRVQYYNYYW
                     SFYRFEDFDCYFNEFGNELAQYTAAYATKKLDEIEGYFDYNLEKRIIFIIHNKANEYR
                     QSNIGLLTFDEDSYNTGGYTRIIKNKVMLYYEGDHISYQKQIAESITEVIINEMLYNA
                     DFRDRISSSSLITMPDWFLKGLTRYIAFGWDYETENKVKDGIKSGKFRKINHLESEDA
                     LVAGQSFWRFIARKYGDAIIPNILYLTKIYKNVDDGFLYVLGIKLKDLLKEWKQYYTD
                     EYSSGNNLPEDDISTIRKSRKEQIYQQVRVSPDGKYLAYVTNDWGRKKIFLYDQATGK
                     NRAIFRIEPKYEHKTDNTYPVIGWHPSGRILTFINEEKAGMVIYFYRIDEKKTEERNL
                     LYFDKVMSFSYSPDGSRLVLSGVKDGMTDIFVHTIASGVNDQITRDVADDLDPSFIGD
                     KPGEIIFSSNRLSDTLINDGNPFEKTGLVFDLFTYNVDKKNNILTRLTEGQYASRYEP
                     EPVAGNRITYLGDNTGIMNRYTARFDSTVSFIDTTTHYRYFMRSTPVTNYDRNILNHS
                     FSDASDIYGEVVYKNRKFNLRRKPLSASPPVPQNEITLTPFRKEQNRLFHKADSIETI
                     RKWLLAEDKKRRDTLKKPLYEYYIKDEPIDINHYIFEKEKQNYYEQLWRKDYMDIDLD
                     TGRLNLPLIRIYETSFYNNYIANQIDFTFLNNSYQVFNGGAPYFNPGINLLTRFGILD
                     LFEDYRLTAGFRFSGNFDSNEYLLSFESLKGKFDKQITFHRQAYFSTDNRDLFKTHTH
                     NLYLSLAKPITPVLALKGSLLYRYDRHVTLARDLPSLSSETISKHWASLKGEIIFDNT
                     RKRGINIYYGTRFKVFAEYYRQLTSRKSDMFVLGADFRKYTKIHRELIWANRFAASTS
                     FGPTKLVYYLGGVDNWMGYLFNKGEMFDKSIPVSNLENYGFQALATNMRGFRQNIRNG
                     TNFAVINSEIRWPFVRYFAGHPLRSNFLNSLQIVGFGDAGTAWTGADPWSGMNSWDKE
                     TYKSGPITVVLDSNRQPIVYGVGVGLRAQIFGYFIRADWARGIENGYLLPRIFYLSFS
                     LDF"
     gene            7534..7704
                     /locus_tag="PK288_03340"
     CDS             7534..7704
                     /locus_tag="PK288_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM17984.1"
                     /translation="MAYVINDDCTACGTCIDECPVEAISEGDIYKIDPDICTDCGACA
                     DVCPVEAIHPAE"
     gene            complement(7755..8774)
                     /locus_tag="PK288_03345"
     CDS             complement(7755..8774)
                     /locus_tag="PK288_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17985.1"
                     /translation="MRFIRLILITGLICVSGLSCRKNPYRVNISGIKTEIGIKRLEDD
                     LFTLDPDSIQAKVPVLKQKYGHFLQLFSYVINTGDIDSPAFPDFLFRFCTDKQNYDVY
                     EMTRQLYPDVADIEKGLKGAFRHYLYYFPGRIVPAVFTCITGFNNSIITGDSVLGIGL
                     DRYLGPDCKYYPRLEIYSYMAARMTPENIVPDCMYGWASSEWDFESAGYETDNVLAEI
                     LHEGKLKYFEKCMLPDLPDETVFRFTPGQMKFCRNNEARMWQYLVENDLLFKTDRFVV
                     RKLTGEAPFTSYFTNQSPGRAAVWIGFRIIESYMRKNPAVSLKDLMTETDVQKILEKA
                     RYSPQ"
     gene            complement(8899..9804)
                     /locus_tag="PK288_03350"
     CDS             complement(8899..9804)
                     /locus_tag="PK288_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPM17986.1"
                     /translation="MRKIIGNIILALFIVFQSAYPKSNPERIVFIALDGISIEGLQKA
                     RTPNIDRLMHEGSYSLKTRVVMPSVTLPNWTSHLTGSGPEQHGVTDNGWTIDKHVLPA
                     VEKDDKGYYPSVFKILKDQVRGIQIGFYYNWENLIYPYNRSYLDEVSFLEDDAYTGNF
                     DRAFEFIRKNREKPTFVFLYTVHTDANGHNHGWMSPEYIRSIEEADTEIGRLIAKMEA
                     EGIYNGTYFIFTSDHGGVNKGHGGVTPTEMEVPWSLTGPRVIKNREITTPNNTVNSAF
                     VIAHIFRCNTPECWTGKMIPTLFGK"
     gene            10245..10796
                     /locus_tag="PK288_03355"
     CDS             10245..10796
                     /locus_tag="PK288_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HPM17987.1"
                     /translation="MKKTSLAIVVLMCFISYNAKAQFVAGGTLGLQAPVGYFGDWAKV
                     GVGINLSGQYMLKENMAVGLNIGFNRFGGQDFGFGDGDHNYSYSMIPITGLFEYHFTV
                     SSIKPYAGLELGFYHMGFRVKYGREVERDGEFYFGLAPTAGVLYSINDNLILCGNLKL
                     HNVFAEGDNLTWIGINAGVRIPF"
     gene            11100..12752
                     /locus_tag="PK288_03360"
     CDS             11100..12752
                     /locus_tag="PK288_03360"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPM17988.1"
                     /translation="MKISIAQLNYHIGNFEKNKGLIIKAIDKARSEGSDLVVFSELCV
                     PGYPPLDLLDRIDFIEKCNRTVNEITDHCKGIAAVIGTPVYNKEPGGRKLYNSALVLS
                     DGMTIFSANKSLLPTYDVFDEYRYFEPGKEFRLLSLKGINIALTICEDLWDDQPHGNW
                     PGSAGIYPVAPMEELAVFNPDLVINISASPFSYQAVETRRNIFVAKALKHRIPVISVN
                     QTGANTGLIFDGASLAVNRNGQIFRQLGYFEEAVETFSLEDLDAGFNFPDIGRAETAA
                     LIHKALITGIQDYFAKTGFRKAILGLSGGLDSAVCLCLAAEALGPDNVLALLMPSRYS
                     PGSSVNDSVVLAEKLGIKYQLISIEEPMRAFEKTLKGPFKGLKPDVTEENIQARIRAI
                     LLMAYSNKFGYMLLNTSNKSETAVGYSTLYGDMAGGLSVIGDVYKSDVYKLASYINRN
                     GETIPEAIIRKPPSAELKPGQLDTDSLPPYDILDPILYRYIELQKPPDEIVAEGWDRG
                     IVEKVIRMINVSEYKRFQAPPVLRISSKSFGDGWKMPLAARY"
     gene            complement(12825..15290)
                     /locus_tag="PK288_03365"
     CDS             complement(12825..15290)
                     /locus_tag="PK288_03365"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HPM17989.1"
                     /translation="MFCISTSDIARITGGTLYGPSDTTITDISTDSRQVSFTSDVAFI
                     AIIGKNHDGHLFIEGLSKKGIRVFLVSRLPENIGNYTGTSFILLNDTVDGLQKLASFK
                     RSLYNFPVIAVTGSTGKTIVKEWLADILGRTEAVVRSPRSYNSQIGVPLSVWKMDERF
                     RYAVFEAGISMPGEMEKLRNVIQPGIGIITNIGEAHSSNFNDNRAKAREKLGLFTDCS
                     MIIFCADHEVIRELVCEDNNLSGKNLVSWSFHDEGATVFVKSRLNETGLTSLEVSYKA
                     GTEVFEIPFSDRASVENAVTVASACLALGIDRDIIRKGLSQLQAVAMRMEIKGGINNC
                     LLIEDYYNSDPGSLGMALEFMKSQNGRTSILILSDFVQSGRNEEELYGETAELIRKTG
                     INRLIGIGPSLMRCRRLFEPGSGFYASTDDFERHFSSRDFRDSVILIKGARMFEFERI
                     ARLLEQQAHITVLEVSLDAITHNLNEIRRLLNPGTRIMAMVKAFAYGAGSAEIAGILE
                     YNRISYLAVAYPDEGAELRNAGVSLPIMVMNPDPASYDVLIRYNLEPEIYSLSALEKF
                     RESASRHGLFQYPVHIKIDTGMHRLGFLVSETDRLISVLKQADCLKVASVFSHLSGSE
                     DPALDEFTHHQASLFEQVSGKINTALGYSFLRHLLNTSGIIRFPRYQYDMVRPGIGIY
                     GAGNYEGMNLRPAGRFMTAISQVKYISPGEPVGYGCASVSDRERKIAILPVGYADGLN
                     RRLGNGKGSVFIKDRRYPLTGNICMDMCMADITGSDIEEGCAAEIFGENISINEVARI
                     CGTIPYEILTSVPARVKRVFYRE"
     gene            complement(15292..15879)
                     /locus_tag="PK288_03370"
     CDS             complement(15292..15879)
                     /locus_tag="PK288_03370"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005828444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPM17990.1"
                     /translation="MMEFLENSVRMKDRRGSVEVITGSMFSGKTEELIRRLRRAQFAG
                     LRVEIFKPSIDKRYSDTRVVSHDDRSILSTLTDNASSILLLSGNADVIGIDEAQFFDM
                     SIVDVCNTLANNGKRVIVAGLDMDFMGNPFGPMPALLAIAEYVTKLHAICMRCGNLAQ
                     YSFRKSDESRVVVLGEKDKYEPLCRSCYNMSLKRN"
     gene            15991..16719
                     /locus_tag="PK288_03375"
     CDS             15991..16719
                     /locus_tag="PK288_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM17991.1"
                     /translation="MDLKTTFDIIIKDLNDIREIIDDLKQYQGVPAIEIELAKSKCRS
                     VSEIISFLRNSDLSHVGRKQDISEKREESRSHQKDPVLSAKKPVEIQVEKPAFHEPVK
                     VDAPQDKPISPVVVTERPEPVPVPKAAEYTIIADQFANRPESFHEKLGGMKPEDDVLE
                     IIKTKPVSSISEAIGINDKYLFIKEIFNGDQETYNRVLSELESAGSFDDAKSIIMDRA
                     GINKENEAFRMFISILKRKFPSNE"
     gene            16712..17386
                     /gene="rsmI"
                     /locus_tag="PK288_03380"
     CDS             16712..17386
                     /gene="rsmI"
                     /locus_tag="PK288_03380"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPM17992.1"
                     /translation="MSKLILVPTPIGNLRDITYRAVEILKGVDLILAEDTRQTGKLLS
                     FYNIRTPIQSHHMFNEHRSVEAISSRILSGTTVALVSDAGTPGISDPGFLLVRKCIEK
                     GIGVESLPGPTALIPALLNSGLPSDRFCFEGFLPPRKGRNKKLEVLAREPRTMVFYES
                     PFRLIKTLDDLALFCGPDREASVSRELTKIHEETIRGSLSFLAEHFREKTPKGEIVIV
                     VAGRKL"
     gene            17383..20097
                     /locus_tag="PK288_03385"
     CDS             17383..20097
                     /locus_tag="PK288_03385"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 and carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPM17993.1"
                     /translation="MKQKILCLTLKARKIFIAAILTGVPFISIHTQILKGRITDRSGE
                     PVQYATVYFQELKHGTTSNPRGDYEIRLPDGKYSVIYQSLGFEPFISEVTIKGDTIVR
                     NVVLVEQYYQIPEVLITEKGEDPAYYIMRRVIGLAPYHLNHVSYYKAEVYLKGNLHIK
                     KIPRVVQRSMRMGSRNRASEPPDMQVKIREGDSFFLESFNEIDFKAPDNYVQRVISFK
                     STFPGQDDNITPMDYIKASFYQPVIARIAISPLSPSAFSYYRFKYLGSSLQGDHSVSK
                     IEVTPKHRSPQLFQGTLFVVDDVWCLHSVDLTNENMAGKIHIRELHIPVQDEIWMPVS
                     HYFEMNMGIMGFKADVNYTSSVKYMTVEANTALKKPDPLTGTYSDQVTAADTLMTKSR
                     GKIEEILRKEKMSNRDMIRLADLMNRESSASLRDSLPKSLEIKDKTQYIIEKDAGKKD
                     SSFWAGIRPIPLSEAELKTLGTKPGTGSLIKTAGDSSAMAEQRKRTPFIRTLRHIAFG
                     HTWADTTGWRISGGDLVNTRCSGFNTVDGFILGTDIRITKEFRNKWSFSLYPEIRYAF
                     SREKLMGRANMNINSGGMKPVRFFMQSGITSRDISTGGGIPPLLNSATSLLLKKNYLK
                     LYESRYLNLGLGFEIKNGFNVELKSGIDNRHILENTTDFSLFRLSREYTDNIPDNRYL
                     NLEENSPSRYPFNDQKQISFSAKLTVVPYQKYRIIRGTKVPEGSQWPTFRFFWEHFMN
                     SVSPGEAKYAHFNMLKFEASQKLSPGAYREFKWLFRTGGYTDNRGISFFDFFHFNNQS
                     FPLLLYDHDDAFMIPRYYTLSTPEFFAEAHLKYTSPYLLMKYLPVFNRTLMRENLVFS
                     YLGSRYSNSYTEIGYSVSEIFLIAELGVYAGFENLSYKFTGIKISFRF"
     gene            complement(20176..21702)
                     /gene="dnaB"
                     /locus_tag="PK288_03390"
     CDS             complement(20176..21702)
                     /gene="dnaB"
                     /locus_tag="PK288_03390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPM17994.1"
                     /translation="MAKSKTVSGPAIISLPDYGRVPPQAIDMEEAVLGAIMLEKEAVI
                     TILDILKPESFYKEAHQKIFKAVSDLSMREHPVDLYTVTEELRATNELDSVGGPVYLT
                     QLTSKVVSAAHVDYHARIVAQKYIQRELIRVSTEIQVRSYDDSYDVTDLLDYSENALF
                     QIAEGNIKREVSPINLVIKEAIREIEEAGKREDALVGIPSGFTKLDRLTSGWQKSELI
                     IIAARPSMGKTAFALSMARNMAIDHGKKVAIFSCEMSSIQLVNRLIIAETDIPGEKIK
                     NGRLSEEEWKQLDSRIKKLVQAPVFIDDTPAISVFELRAKCRRLMAQQKLDIVIVDYL
                     QLMSGPDNAGSREQEVSSISRSLKSIAKELNVPILALSQLNRSVEIRGGSKRPLLSDL
                     RESGAIEQDADMVVFIHRQEKFGMLTFEDGSSTRGIAEIILAKNRNGPVDDVRLRFRE
                     EKAQFVDIEDFETDLITDIREGSQSFTIGSKMNHDISRNRSFNDFEHETDLLNEPPFM
                     "
     gene            21914..22732
                     /gene="ispE"
                     /locus_tag="PK288_03395"
     CDS             21914..22732
                     /gene="ispE"
                     /locus_tag="PK288_03395"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPM17995.1"
                     /translation="MVIFPKAKINLGLRITARRPDGFHDIETIFYPVGLCDILEMTVN
                     DDKKGKDIFSLSGLGLDGDQDGNLVIKAVRKLREAYDLPPLKIHLHKIIPPGTGLGGG
                     SSDAAGALRTMNRVFELALSAQELKTIALDLGSDCPFFIDCQPAFATGRGENLRQVDA
                     VLEGLYIVLVNPGIPISTREAYLKCSPSKTGVNLSELIHYPVAEWKSHIINDFEKTVF
                     EQYPQIGIIKRVLYNNGALYSSMSGSGSAVYGIYADKPEIPEILRDYVIYKGIL"
     gene            complement(22797..23126)
                     /locus_tag="PK288_03400"
     CDS             complement(22797..23126)
                     /locus_tag="PK288_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPM17996.1"
                     /translation="MKQTTDKDKRLGILNINSTDYKTRLSTRFLNRKPYQPSDPGTIL
                     SFIPGTIVDILVKRGQKVKKGDDLIILEAMKMQNRLKCPADGTIKKIGVKKGDKVAKG
                     SVLIELK"
     gene            complement(23147..24691)
                     /locus_tag="PK288_03405"
     CDS             complement(23147..24691)
                     /locus_tag="PK288_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPM17997.1"
                     /translation="MAVEKKIRELQEKRSKILLGGGVQAIEKQKAMGKRTARERIMAL
                     LDKNSFNEYDLFVEHDARDFDMENKSLPSDGVIIGSGTIYGAPVAVFAQDFTVAGGSL
                     GLMHSRKITKIMDHALKMRMPLIGINDSGGARIQEGVNSLAGYGEIFFRNTISSGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVDNISKMFITGPEVIKTVLGEEISMEELGGARV
                     HSEVTGNAHFFAKSEDECFGQIKRLISFIPRNNSLRAKVSETHEPLNEYHLDKIVPDD
                     PTLPYDVRDVIMAIVDKSDFFEIMEHYARNIVIGFGRMGGRTTGFVANQPLEMAGVLD
                     VDSSDKAARFIRYCDAFNIPIITLVDLPGYLPGIDQEHAGVIRHGAKVLYAYSEATVP
                     KLTVILRKAYGGGYIAMSSRHLRADFVFAWPTAEIAVMGPEGAANIIFRKEIMSSDDP
                     DKTRKQKVEEYKAKFANPYVAASRGYIDAVIEPSETRRFLLHAMQVSENKTVTLPDKK
                     HGLPPF"
     gene            complement(24874..26013)
                     /locus_tag="PK288_03410"
     CDS             complement(24874..26013)
                     /locus_tag="PK288_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPM17998.1"
                     /translation="MEKRNDKKAFSAVIEFFSIKIIDRYIFRKFLGTFLFCLFLIITI
                     AVVFDFAEKIDNFMEREAPVRAIIFDYYLNYIPYFSTLFAPLFVFIAVIFFTSKMAAN
                     TEIIAILNSGMSFRRMMYPYFLASLIIAIFIFLLTNFVIPQANSKRMVFEDRYYRPSA
                     ARRMPVMNLHRQVFRNVYIYLESFNPISLTGRNFTLEQFDDSGRLQSKLNGNMVRYDT
                     ATNKWSVMTYYIRKIDGNEETIIKGDRIDTTLTIKPNDFSRVPGFEGTMTYRELNDYI
                     DQLQLQGSDELKVFLIEKHKRIANPFAVFILTLIGVSLSSRKVRGGIGKNIGIGLGLS
                     FSYILFMQFASQFSIKGNLGPMLAMWIPNILYACIAVFLYRVAPK"
     gene            complement(25971..27104)
                     /gene="tgt"
                     /locus_tag="PK288_03415"
     CDS             complement(25971..27104)
                     /gene="tgt"
                     /locus_tag="PK288_03415"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPM17999.1"
                     /translation="MVLFEIQSRDRGTNARAGILRTSRGDVQTPVFMPVGTAGTVKGI
                     LQQDLVEEIGAEIILGNTYHLYLRPGTDILKSCRGLHEFMGWEKPILTDSGGYQIFSL
                     ARNRKLSEEGAIFRSHIDGSKHVFTPENTIDIQRIIGADIMMALDECTPFPCDYRYAR
                     KSMELTHKWLDRAVLRYNQSEPLWGFDQYLFPIVQGSIFDDLRKLSAEKIASLSLAGN
                     AIGGLSVGEPAEEMYRILEIVNGILPEDKPRYLMGVGTPANILEAIERGVDMFDCVMP
                     TRNGRNGMLFTGEGVINIRNRKWMDDFNPIDSFGPSRISRHYSKAYLRHLIISGEILG
                     AQIASIHNLAFYMKLVKEARIRIQEGNFREWKNAMIKKLSARL"
     gene            27263..28390
                     /locus_tag="PK288_03420"
     CDS             27263..28390
                     /locus_tag="PK288_03420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM18000.1"
                     /translation="MLIRENLIFNIAFTVFLLSAAIQLFYYFFFFLSAGLSGKAAQSH
                     DYKPVSVIICARNEEKNLRLYLTSVLEQDYPDFEVIVVNDCSEDNSYNVLGELLPAYP
                     HLRISNIARDPKFTHNKKFAQFIGIKAAKNELLLFTDADCHPESDNWIRGMVDGFDEK
                     TDFVLGYGGYSSEKGLLNKYIRFDTMMIALQYFGMALRGMPYMGVGRNLAYRRSVFFK
                     NKGFGQHSHLLSGDDDLFVNKHANRQNTAVETSRETHTRSVPSRTFAEFFRQKRRHLT
                     TAPHYRLRDRIILIAEPATRLLFYISFIILSFSRFWPYAVAIFAGRMIVQSVTFKFAV
                     KKFNEPGIFKYSLLFDIFSPFLNLAFYLSNYLQSPGKNQWK"
     gene            28381..28980
                     /locus_tag="PK288_03425"
     CDS             28381..28980
                     /locus_tag="PK288_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM18001.1"
                     /translation="MEIASNELSEKAKNDLLFVNMARNGDEKAFATLMNRYRDSIYYM
                     LLKMVNNIPDAEDLTIEAFGKAFRNLDSYTPEFAFSTWLFKIATNNCIDFIRRKHAFP
                     SAIQQSQDDLESITRNIQSDLPDPEETLINDQKIAVLRSVVNQLKPRYRQLIELRYYK
                     EYSYEEIASELNLPLGTVKAQLFRAKNLLYNIFKKTGNR"
     gene            28983..29606
                     /gene="rsmG"
                     /locus_tag="PK288_03430"
     CDS             28983..29606
                     /gene="rsmG"
                     /locus_tag="PK288_03430"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009752102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPM18002.1"
                     /translation="MRDIIRYFPFVTAVQTDRFGQLREIYEYWNSRINLVSRKDMDNF
                     YIHHVLHSLSIAKIIRFLDGTSILDVGTGGGFPGIPLAIMFPGCKFFLLDSIGKKIKA
                     VAGVAETLGLENVTVVRKRAEDETERFDFAVSRALMPIPGFVNLVTKNITPAGRNTLE
                     NGIIYLKGGDIDEEIRPFRNKVKIWNISNYFDEPYFETKKVIYIPVF"
     gene            29695..29850
                     /gene="rpmH"
                     /locus_tag="PK288_03435"
     CDS             29695..29850
                     /gene="rpmH"
                     /locus_tag="PK288_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPM18003.1"
                     /translation="MKRTYQPSRRKRANKHGFRERMSTPNGRRVLAARRAKGRKKLTV
                     SSELKLK"
     gene            29999..>30938
                     /locus_tag="PK288_03440"
     CDS             29999..>30938
                     /locus_tag="PK288_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HPM18004.1"
                     /translation="MINPEKYISDPALLKIAEKITDEKRITPEEGLTLYQNAGLPLLG
                     LLAGIVRRRINGENAFFIRNFHIEPTNRCIYNCRFCSYRKPPKHPESWDLSHEQMLET
                     VKRFDGRQIEEVHIVGGVHPEHDLHYYGELIRQIKQYRPGLHIKAFSAIELDYMIRKS
                     GYSLEEGLGILKDYGLDSIPGGGAEIFDEQIRKIICPEKASSDMWLRIHETAHLAGIP
                     SNATMLYGHMETYSHRIDHMERLRLLQDKTQGFNAFIPLKYKRENNPMSHLGEVSMIE
                     DLRNYAVSRIYLDNIPHLKAYWPMTGKEVAQLSLSFG"
BASE COUNT         8661 a         7125 c         6634 g         8518 t
ORIGIN      
        1 cagcaacagg aacaagctga tgacaaggga ggatatcctc accgagatat ggggagagga
       61 tgattacttc cttggaagga gcatggatgt ctttataagt cggctgagga aatacatatc
      121 cggcgacaga aacatcgaca ttcgcacaat acgcggtacg ggttttatcc tggaagaaat
      181 tcgtccgggg gaacctgatg gagaaaggtt ttagaaaaaa cccttactgt ctgctgtcaa
      241 ggatttcttt taccttgtta tacagatcat tcccggtaag gttcctggca atgattattc
      301 cgttgtcgtc gagcaggaaa tttgcaggga ttgaatttac cacgtatagc ctggcagcag
      361 cgttgttcca gtattgaaga tcggaaacat gcgtccaggt gagcttatca tcggcaatgg
      421 ccttcagcca ttcttccctg tcgcggtcga gcgacacacc aaggatgtca aatccccttc
      481 tgtggaattc attgtagatt ctgacaacat ttggattttc cctccgacag gggccgcacc
      541 atcctgccca gaaatcaacg agaagaagtc tggagccgat ttttgacgaa agactcacgg
      601 ggttccccct ggggtcgtta agtgtaaaat caggggcctt ctgtccgatt gaaaccggtt
      661 tcatcatggc aaccagttct gttaattcct taacagcagg agtattcttt aaagcagtgt
      721 cgaaggcatt tatcagtgtc tcaagctcag ctgcctccat ctcacggctc agccctgcca
      781 ggattgaagg agacacaaag gaagaaggat tgttcttaac gaaatctttc tgcagattga
      841 tcatcgattc ctgaagcgac agtatttcat tctcgaggat cctcagtctt gcagtgtcat
      901 tttcctggaa cgcgctctgg taatcattgt atgccctgga atattgatca ttcagggcct
      961 tgtttgaatc aacaaacctc ctgtactcat cctgtgtctt tgatccggtg atttttgcat
     1021 tgaacagcga atcgagcctt cccctgatct gaatatttga attctcaaga tagaatgtag
     1081 tcctcatcct gctgtctttt actgtaagca gtaccatctc aggatattta accggacctc
     1141 cctgcatttt gaaattccct ttttttgata cagccgaatc gattgtcaca agttttccat
     1201 cggctctttt ttgaagaagg aaagtaacat tatcggcacc ttctatcttt ccggtaatct
     1261 tatattgagg gccggatgaa caggaggcca caatgatcgc tgaaatcagg aatgcatatg
     1321 cttttttcat tttcttgtct tttgtcagtt tctaaaaatt gagcaaagat agacaataat
     1381 gtttatggtg ctctttttat gtgatattta tctaacaggg caaatgccgc attatgagat
     1441 gtgattttcc cttcataaag ctgcttttcc agacttttca ggagtggctt tattttcttg
     1501 tcggaataaa aagaactggt gagactttca gagattattg catgcatcag tgtcacttcc
     1561 tgttctttgc ggagtttctc aaaatatccc ccggacgtgg tgtgtctgac atattccagg
     1621 atattgtccc ataactcagc aattcccgag ttttggtgag cggagcaggt cattactgca
     1681 ggtttccaac ccgatgtcgg taaaggaaaa agattcattg cattctgaaa agaaatcctg
     1741 gcacgctcag cgttcagcct gttgggtccg tcagctttcg ttaccgcaat accatcggcc
     1801 atttccatta ttcctctctt gattccctgg agttcatccc ctgcagaagc agataaaagc
     1861 aacaggaaaa aatcgaccat tgtccgtacg gctgtttcag attgtccgac tcccaccgtt
     1921 tcaacaataa ttgtatcaaa accggctgct tcgcacagga ttattgtttc ccttgttctg
     1981 tcagcaactc ctccgagggt ccctgccgaa ggagatggcc tgatatatgc attttcctgt
     2041 atacttagtt tttccattct ggttttgtcg ccgagaatgc tgccttttgt ctgggcactg
     2101 ctcggatcaa tggcaaggac agcaatcttt ctgcccaatc gtgtcagatg caatccgaac
     2161 gattcaataa aattactttt acccacacca ggggctcctg ttattccaat ccttaccgac
     2221 cgcgaactca ggggaaagca tttgtctatt atctgcctgg cgatttcagc attatcgggc
     2281 agcgtactct caataaatgt tatagcctgg ctcaggatgg tcctgttgcc cgaaaggatg
     2341 ccctcaacat attcgtctgc cgaatatttt ctttttttct ttttccccga gggagaaaag
     2401 gcaacaggat taacttccgg cggataatta actgcaggtt gaacttttgc agcattcttg
     2461 aaatcctctt tgtttttcat ctgtctgaaa ctcttcatgc aaaattaaaa aaagggtgcc
     2521 cctcttcagc cggcacccat ccagaagtca aatatctttc agtgtgattt tctatttgct
     2581 cgcagagact tcctgggcca catctgctgt gaccatcagc attacctccg aatttttggg
     2641 atcattggta tatacatata ttgcctttgt aacttttccc ttgtagccac ccgagttaaa
     2701 ggtagctttg atcgagcctg attcgccggg cttgataccc tgtccctgta ttccctgctg
     2761 aactgccgta caaccgcatg atgacctgat atgtcttatg acaagatccc ttttccccag
     2821 gttggtgaac ttgtattcaa cctcattttg cgtggaacct gctatctttc caagatcaat
     2881 cgtcgttttt tcaggtttaa ataccggtgc attggccagt tcctcctttg acaagccgga
     2941 aaaatcctcc acgagattgg ctgatacata gtaatataca ttattatgaa gtaccccgtt
     3001 agtcttaacc cttaccatgc tgcttacatt accccaggtc tggttctttg tggcatcata
     3061 agtaccctca actatcccct tctcgcctgc cttgagagtt tcaggcttaa ccttaaggga
     3121 aagataatca ggaactcctt cgaattcaac tttaacagga gaatttgatg tattgatcag
     3181 ttgcattacc ctgatctttt tttctgtctt tttgacattg gtaaatgcca ggtgattgct
     3241 ttcaaatctc actgatccga caggaaaagt aaaaatctct tcaagtgttt tttcccgcgg
     3301 gatcacttcc cctttcagca aaatgaccac cacttcgggc tttgaatttg aataaaccga
     3361 cagtgtctta ttgaagggac ccggtctgtt tgccggatca taaatggcag taatggtacc
     3421 tgtacctttc ggaggtatgg gtgtttttgt ccattcaggc aatgtacatc cgcaggaagc
     3481 aacaatattc tgaattacca gcggctggtt tccagtattc atcacaacga aatcatactt
     3541 ctgccgtcct gcttcttccc tgaactttcc aaagtcatgc tctgtaccgg aaattttcat
     3601 tacaggctgg ccccacgata aaagaccgca aaatgccagg gcaaaaaata aaaataactt
     3661 tttcatatat gaaaactatt ttgatgtaaa tgttcctttg actgtatccc gaaatgggaa
     3721 gcctgctgtg agtttaagat tctccgttcc cgctgaattc cttcaatcct gcccgtcggg
     3781 cagcatctca aaaacatgat ggtttcattt cggagattaa ttaacctggt tttcactgtt
     3841 aaagatcaaa caatgagttc aaatatctga attattttaa aactgacatt ccagcaattt
     3901 tattgcccaa aataacaaaa accagaccaa attcgggaaa agaattctct cagtgtaaaa
     3961 ttaataaagt ttgatattaa tcttaatttt aatgaagaaa atcttagttt tgtcctctgc
     4021 aggaagtcat ttggaataaa atttgaagcg gaaaccgttt tgcggagcga gaattttgga
     4081 gatgaaaaat acccggagac ttatttttat ccttgcagcc atgctccttt tcttcagggc
     4141 ggaaggacag ttctataacg gactccagat gcccttcggg aaaaacaggg tacagtatta
     4201 taattattac tggtcatttt acagattcga ggattttgac tgctatttca atgaattcgg
     4261 caacgaactt gcccagtata ccgccgccta cgccacaaaa aagcttgacg agatagaagg
     4321 ttattttgat tataaccttg aaaagagaat catcttcatc attcacaata aggccaacga
     4381 gtacaggcaa tccaatattg gtctcctgac ctttgacgag gacagctaca acaccggagg
     4441 atatacccgt ataatcaaga acaaggtaat gctttactat gagggagatc atattagcta
     4501 ccaaaaacag atcgccgaat caataacgga agtcataatc aatgaaatgc tgtacaatgc
     4561 tgattttcgc gatcgtattt ccagctcttc ccttatcact atgcccgatt ggttcctgaa
     4621 aggcctgaca cgctatattg cattcggatg ggactatgaa accgaaaaca aggtcaagga
     4681 tgggatcaaa tccggaaaat tccggaaaat aaaccatctt gagtcggaag acgcacttgt
     4741 tgcaggccag tccttctgga gattcattgc caggaaatac ggcgatgcca ttatccccaa
     4801 cattttatat ctcacgaaaa tttataagaa cgttgatgac ggcttcctgt atgtcctggg
     4861 cattaaactc aaagatctgc tgaaggaatg gaaacaatat tatacagatg agtattcatc
     4921 cggcaacaat ctccctgaag acgacatttc caccatcagg aaatcccgga aagaacagat
     4981 ctaccagcag gttagggtca gccctgacgg aaagtacctg gcatatgtaa ccaacgactg
     5041 gggacggaaa aaaattttcc tgtatgatca ggctaccgga aagaacaggg ccattttcag
     5101 aatcgaacca aaatacgagc acaagaccga caatacttat cctgtaatcg gctggcaccc
     5161 ctcggggagg atacttacat tcatcaatga ggaaaaggca gggatggtaa tttattttta
     5221 ccgcattgat gagaaaaaga cagaagagag gaatcttctt tattttgaca aggtcatgag
     5281 cttttcatat tctcccgacg gttcccgcct tgtattatcg ggtgttaagg atggcatgac
     5341 tgatatcttt gtgcacacta ttgcctcagg agtgaatgac cagataacca gggatgttgc
     5401 cgatgacctt gatccttcat tcatcggaga caaaccgggg gaaatcatat tctcatcgaa
     5461 ccgcctgtcg gacactctga taaatgacgg caatccattt gaaaaaacag gactggtgtt
     5521 tgacctgttc acatacaacg tggataagaa gaataatatt cttacccggt tgacggaagg
     5581 acagtatgca agcagatacg agcctgaacc agtcgccgga aacaggatta catacctggg
     5641 cgacaacacc ggaataatga acaggtatac tgccagattt gacagtacgg taagttttat
     5701 cgacacaaca acccattaca ggtacttcat gagatcaacg cctgtgacaa actatgacag
     5761 gaacatactc aatcactcat tttcagatgc ttctgacata tatggagaag ttgtgtataa
     5821 aaacaggaaa ttcaatctga gaaggaaacc gctatcggct tcgccgcctg tgccgcaaaa
     5881 cgaaatcacg ctgacaccat tcaggaaaga gcagaacagg ttatttcaca aggctgacag
     5941 cattgaaaca ataagaaaat ggctcctggc tgaagacaag aaaaggaggg ataccctgaa
     6001 aaagcctttg tacgaatatt atatcaagga cgagccaata gatatcaatc attatatatt
     6061 cgaaaaggaa aaacagaatt attacgaaca gctctggcgc aaggactaca tggacataga
     6121 cctcgacacc ggcaggctga acctgcctct cataaggatc tatgaaacat ccttttacaa
     6181 taattacatt gccaatcaaa ttgattttac ctttttaaac aactcttacc aggtctttaa
     6241 cggaggtgca ccctatttca atccagggat aaatctcctt acccgtttcg ggatacttga
     6301 cctgttcgaa gattacaggc tcacagccgg tttcaggttc tcgggcaatt tcgacagcaa
     6361 tgagtatctg ctcagctttg aaagcctgaa gggcaaattc gacaaacaga ttactttcca
     6421 ccggcaagcc tatttcagca ccgacaatag agatctgttc aaaacccata cacacaatct
     6481 ttatctttcc cttgccaaac ccataacacc ggtgctcgcc ttaaagggca gcctcctgta
     6541 cagatacgac aggcatgtaa cactagccag ggatcttcct tccctgagct ctgaaacaat
     6601 ctccaaacac tgggccagcc tcaaggggga aattatcttc gacaataccc gtaaaagagg
     6661 aatcaatatc tactacggaa ccagattcaa ggtctttgca gagtattaca ggcagcttac
     6721 cagcaggaaa tcagatatgt ttgtacttgg agccgatttc aggaaatata caaaaataca
     6781 cagggagctg atctgggcaa accgctttgc agcaagcacc tcattcggac cgacaaaact
     6841 tgtctattat cttggaggtg tggataactg gatgggatat cttttcaaca aaggtgagat
     6901 gttcgacaaa agtatcccgg taagcaatct tgaaaattac gggttccagg cgcttgccac
     6961 caacatgagg ggattcaggc aaaatatcag gaacgggacc aattttgccg tcatcaacag
     7021 tgagattcgc tggccctttg taaggtattt tgccggtcac ccgctccggt caaattttct
     7081 gaacagcctt cagattgtag ggttcggcga cgccggaaca gcctggaccg gggctgatcc
     7141 ctggtcaggc atgaactcgt gggacaagga aacttacaag agcggaccaa ttacagttgt
     7201 tcttgattca aacaggcaac ccattgtcta tggtgtcggc gtcggactca gggcccagat
     7261 cttcggatac tttatcaggg ccgactgggc caggggaatt gaaaacggct acctcctccc
     7321 aagaatattc tatctgtcat tcagcctgga tttctgattt gccgcagaag caacatactg
     7381 attacctgcc tggaaaaaac aactccggcc aatagcatta tcggtaacag cttaaagaaa
     7441 atccgcaatt attgcaaaga tccttcttca tttggaaaat ttggaatact tttgcagcgc
     7501 ttatgttttt attataacca aaaaagtata aagatggctt acgtaatcaa tgatgattgt
     7561 acagcttgcg gaacatgtat tgatgagtgc ccggtcgaag cgatatctga aggtgatatc
     7621 tacaaaattg atcctgatat ctgtacggat tgcggtgcat gtgccgatgt atgcccggta
     7681 gaggcaattc atcctgctga atagctttac aatcagaaag ttttaaaaaa aggtgcctct
     7741 ctgaggcacc ttttttattg aggactgtac ctggcttttt caagaatctt ctgaacatct
     7801 gtctctgtca taagatcttt cagcgatacc gcaggatttt tccgcatata cgattcaatg
     7861 atcctgaatc cgatccagac agccgccctt cccggtgact gatttgtaaa atagctcgta
     7921 aaaggagctt ctcctgtgag cttccttacc acaaatctgt cggttttgaa cagcagatca
     7981 ttttcaacaa gatattgcca catacgggcc tcgttgttcc ggcagaattt catctgcccc
     8041 ggggtaaacc tgaaaacagt ttcatccggc aaatctggca gcatgcattt ttcaaaatat
     8101 ttcagcttac cttcatgcaa tatttcggcg agaacattat ccgtttcgta accggctgat
     8161 tcaaaatccc attccgacga agcccagcca tacatacaat cgggaactat attttcgggt
     8221 gtcattcttg ccgccatgta actatatatc tcaagccgtg gataatactt gcaatcgggc
     8281 cccaggtacc tgtcaagtcc tatcccgaga acggaatcgc cggtaataat actgttgttg
     8341 aatccggtaa tacaggtaaa taccgcaggc acaattctgc ccgggaaata ataaaggtaa
     8401 tgcctgaatg ctccctttag tcccttctct atatcagcaa catcggggta aagctgcctg
     8461 gtcatttcgt aaacatcgta gttctgttta tcggtacaaa accggaaaag aaaatcagga
     8521 aaagccggac tgtcaatatc tccggtattg atgacatagc tgaaaagctg aagaaaatga
     8581 ccatacttct gtttaagtac gggaaccttc gcctggatcg aatcaggatc gagtgtgaaa
     8641 agatcatctt cgagacgttt aatccctatc tcagttttga tacctgaaat attcaccctg
     8701 taaggatttt tcctgcacga caaacctgaa acgcaaatca ggcctgtgat caggatcagt
     8761 ctaatgaatc tcattgttgt tgagtctgaa atctagcatt aaacgtgccg ggaatatatt
     8821 cccgccacgg gttccaaaat taatgaaccg gaaatcagta aacaatatat ttttgcatga
     8881 aaggatgtaa cccctgtcct actttccgaa gagtgtaggg atcattttgc ccgtccagca
     8941 ttccggtgta ttgcacctga agatatgtgc tatcacaaat gccgaattta ccgtattgtt
     9001 cggggtggtt atttcacggt tctttattac ccgggggccg gtaaggctcc agggaacctc
     9061 catttcagta ggtgtgacgc ctccatgacc tttgtttaca cctccatggt cagaagtaaa
     9121 tataaaatat gtgccgttgt atatcccttc agcttccatt tttgcaatca gcctgccaat
     9181 ctcagtatcg gcctcctcaa tcgatctgat gtattcgggc gacatccagc catgattgtg
     9241 cccgtttgca tccgtgtgaa ccgtatacag gaatacaaaa gtcggttttt ccctgttttt
     9301 cctgataaac tcaaaagccc ggtcaaaatt accggtatag gcatcatcct caaggaagct
     9361 cacttcatcg agatagctac ggttgtaagg ataaatgaga ttctcccagt tatagtaaaa
     9421 accaatttgt atcccgcgaa cctgatcctt cagtatttta aatactgatg gataatatcc
     9481 cttgtcgtct ttttccaccg cgggcaaaac atgcttgtca atcgtccatc cgttatctgt
     9541 tacaccatgc tgttccggac cactgcccgt cagatgactt gtccagtttg gaagggttac
     9601 ggagggcatt accacccttg tttttaatga ataggacccc tcatgcatta atctgtcaat
     9661 gttgggtgtg cgggccttct gaagcccttc aatgctaatg ccgtccaatg caataaatac
     9721 tatacgctca ggattgcttt tagggtacgc actttgaaat acgataaaaa gagcaagaat
     9781 aatgtttcct atgattttcc gcatgatcgt taaaggtttt aatttatact gactgaatga
     9841 caataatcaa taaaattcca gctatttatt tgatgatcaa actattatct gaacaactca
     9901 acacgttttc gtcatttaca atatacaaat ttgttcctgt caggaaagct ttcgtcatag
     9961 aaatctgcac cgaaaggtta aaaattcggc aaaaaaataa agtcaaatca gaaaattccc
    10021 aaatagctgc ccgggtttaa ccgggtattc ttatccggac tgcccggttc aggggggatt
    10081 aggatatcct ggacaggaaa taactataat atttccggct tatggtccgt ttgcctaaca
    10141 ctattccgta tgcaagtaga taagggagga atctgaaaat cctgaaaaga gtaagatgtg
    10201 gccgctgaaa aacacaagag taagcaatat ttaattcttt tcctatgaaa aaaacttcat
    10261 tagctatcgt tgttctgatg tgtttcattt cgtacaatgc caaagcgcag tttgttgccg
    10321 gcggcaccct gggcctccag gcacccgtcg gttattttgg tgactgggct aaggtaggag
    10381 tcggcataaa tttgtcaggt caatacatgc tgaaggaaaa tatggcagtc ggcctgaata
    10441 ttggcttcaa ccggttcggc ggacaggatt tcggttttgg cgatggggat cataattact
    10501 cctattcgat gattccgatc acaggacttt ttgagtatca ttttacagtc agcagtatta
    10561 aaccctatgc cgggctggag ctgggttttt accatatggg cttcagggta aaatacggca
    10621 gagaggttga aagagacggc gaattctatt ttggactggc tcccacagct ggagttttat
    10681 acagcattaa cgataatctt atcctttgcg gaaacctgaa attgcataat gtatttgccg
    10741 agggagataa tcttacctgg ataggcatca atgccggagt cagaatacct ttttaactaa
    10801 cttaatttca gttttccgga taaggccgaa gctcttgcgg catccggaag aagcggtatt
    10861 ggagcggagg ccagcaggcc gccgctcttt tatttgatta agtacctctc gagatcgaac
    10921 ctggcagatc ccttcggatc ttttctcaat cagaataatt gcctgtagtt ttccgccgga
    10981 atacaaccct catatacatc tcccaatatt ttttaatttt gaattatgca gaacaggccc
    11041 ctttgtggtt tttactgtga aagttgccgg aatcaggggg tcaaaagtat aaatagcgta
    11101 tgaaaatctc cattgcacaa ctgaattatc atattggcaa tttcgaaaag aataaaggat
    11161 tgatcataaa ggcaatagac aaagccagga gtgagggctc cgatctggtg gtcttctctg
    11221 aactttgcgt tcccggatat ccgcctctcg atcttctcga tcgtattgat tttattgaaa
    11281 aatgcaaccg cactgttaat gaaataacag atcattgcaa aggcatagct gcagttatcg
    11341 gaaccccggt ttacaataaa gagccaggag gcaggaagct ctacaactcg gctttggttc
    11401 tctcagatgg aatgacgatt ttttctgcaa ataaatccct cctccccacc tatgatgttt
    11461 ttgacgaata caggtatttt gaacccggaa aagaattccg gctcctgagt ctcaagggga
    11521 taaatattgc tcttaccata tgcgaggatc tgtgggatga tcagcctcat ggtaactggc
    11581 ccggctcagc gggtatttat ccggttgctc caatggaaga gcttgcagtc ttcaatcccg
    11641 atttggttat aaatatttcg gcctcgccat tttcatacca ggctgttgaa accagaagga
    11701 atatttttgt tgccaaggca cttaaacaca ggattccggt catttcggtc aaccagacag
    11761 gtgcaaatac cggtctgatc tttgacgggg cttcactggc cgttaaccgc aacgggcaga
    11821 tattccgcca gctgggttac tttgaggagg ctgtggagac attttcgttg gaggatcttg
    11881 atgccggatt taactttccg gatattggca gggctgaaac tgcagctctt atacacaaag
    11941 ccctgataac aggaattcag gattatttcg caaagaccgg cttcagaaaa gccatactgg
    12001 gtttgtcggg gggacttgat tctgccgtat gtctctgcct ggcagcagaa gcgcttggcc
    12061 cggacaacgt actggccctg ctgatgccct cacgatattc gcctggcagt tccgtgaatg
    12121 attctgttgt tcttgcggaa aaactgggta ttaaatacca gcttatcagt atagaggaac
    12181 caatgcgtgc atttgagaaa accctgaaag gtccattcaa aggattgaaa cccgatgtta
    12241 ccgaggaaaa catccaggca aggatcaggg caatcctgct gatggcttat tcaaacaagt
    12301 tcgggtacat gctcctgaac acatccaata aaagtgaaac agctgtcgga tacagtacac
    12361 tatatggtga tatggcgggg ggattgtcgg tgataggtga cgtttacaaa tcggatgttt
    12421 acaaactagc atcctatata aacagaaatg gcgaaacaat tcctgaagcc attataagaa
    12481 agcctccctc agcagagctt aagcccgggc agcttgatac ggattcattg ccgccatatg
    12541 atattctcga tccgatcctc taccggtaca ttgaacttca gaagcctcct gatgagatcg
    12601 tggctgaggg atgggaccgc ggcattgttg aaaaagtgat caggatgatc aatgtaagtg
    12661 aatacaagcg ttttcaggct cctcccgtat tgcgtatctc atctaaatct ttcggggatg
    12721 gatggaaaat gcccctcgcg gccagatatt gaggatcaca ggatcggcat gccggaagct
    12781 tcaatacata aagcaacatt ttaatcatac cgatgtttgt atttttactc cctgtaaaat
    12841 acccttttca ccctggcagg tactgaggta aggatctcat atgggattgt accgcaaatc
    12901 cgggcaacct cgtttattga gatattctca ccaaagatct ccgctgcaca tccttcttct
    12961 atatcggaac ctgttatgtc ggccatgcac atgtccatgc agatattccc tgtcagcgga
    13021 tatctcctgt ctttgataaa aacgcttcct ttcccgttgc ccagtctcct gttaagacca
    13081 tcggcatacc ctacggggag gatcgcaatt tttctctccc tgtcagaaac cgacgcgcat
    13141 ccatagccga ccggctctcc cggggaaatg tatttgacct gcgatattgc cgtcatgaaa
    13201 cggccggctg gcctcagatt catcccttca taattgcctg caccgtaaat gccgatgcca
    13261 ggcctgacca tatcatactg gtaacgggga aaccttatga ttcctgaagt atttaaaaga
    13321 tgtctcagga acgaataacc gagggccgta tttattttcc ccgacacctg ttcaaaaaga
    13381 cttgcctggt gatgtgtaaa ttcgtcgagc gctggatctt cagaaccgga cagatgtgaa
    13441 aaaacggacg ctaccttaag acagtccgcc tgcttcagga cagaaatcag ccggtcagtt
    13501 tctgaaacca ggaatccaag gcgatgcatt cctgtatcta tctttatatg aacgggatac
    13561 tggaacagac cgtgtcgtga agcagattcc ctgaattttt ccagtgcaga gaggctgtat
    13621 atctccggtt ccagattgta cctgatcaat acatcataag atgcaggatc ggggttcatc
    13681 accatgatcg ggagggatac tccggcattt cgaagttcgg ctccttcgtc cggatatgct
    13741 actgccaggt agcttatcct gttatattca aggatcccgg caatctcggc tgaacctgca
    13801 ccatatgcaa acgccttcac catcgccatt atccgtgtcc ccgggttgag caatctcctg
    13861 atctcattca ggttatgagt aattgcatcc agactgacct caagaacggt aatatgggcc
    13921 tgttgttcca ggagtcgggc aatcctttcg aattcaaaca tcctggctcc cttgataagg
    13981 atcactgagt ccctgaaatc gcggctgctg aaatgtcttt caaagtcatc cgtcgaagca
    14041 taaaaccccg acccgggttc aaacaacctc cggcagcgca ttagcgacgg accgataccg
    14101 atcagcctgt tgattcctgt ttttctgatc agttcagcag tctccccgta cagttcttcc
    14161 tcgttgcggc cgctttgaac gaaatcggac aggatcagga ttgatgtcct gccattctgc
    14221 gacttcatga attcaagagc cattcccagg gaaccaggat ctgaattata atagtcttcg
    14281 attagcaggc agttatttat tccgcctttg atttccatcc tcattgccac cgcttgaagt
    14341 tgggaaaggc ctttcctgat gatatcccgg tcaatgccca gagcaaggca ggcggaggca
    14401 accgtaaccg cattctcaac cgaggcacga tcgctgaagg ggatctcaaa aacttccgtg
    14461 cctgctttat aactgacctc cagggatgtg agccccgttt cattaagtct gctcttcacg
    14521 aaaaccgttg caccttcatc atgaaatgac cagcttacca gatttttccc cgataaatta
    14581 ttgtcttcgc acaccagttc cctgatcact tcatggtcag cacaaaatat tatcattgag
    14641 cagtcagtga aaagcccgag tttttcccgg gcctttgcgc ggttgtcatt gaaattactg
    14701 ctgtgggcct ctccgatgtt tgtaatgata ccaattcctg gctgtatgac atttctgagt
    14761 ttctccattt ctccaggcat ggaaatcccc gcctcaaaaa ccgcatacct gaatctttcg
    14821 tccatcttcc agacagaaag cggcaccccg atctgtgaat tatagcttct ggggctcctt
    14881 accactgctt cagtccggcc aagaatatcg gccagccatt cttttacaat cgttttcccg
    14941 gtgctgccgg ttaccgcaat aaccggaaag ttgtacaggg atcttttaaa tgaagccagc
    15001 ttctgaagtc cgtcaacagt atcattaagc aatatgaatg aggttccggt ataatttcca
    15061 atgttttcag ggagcctgct tacaagaaag actctgatcc ctttttttga aaggccttca
    15121 atgaacaggt gcccgtcgtg atttttccct atgatggcaa taaaggcaac atcactggta
    15181 aaactaacct gcctgctgtc agtggaaata tcagttatag tggtatcgga tggaccgtag
    15241 agagtccctc ctgtaattcg tgcgatatct gatgtggaaa tgcagaacac tttaatttct
    15301 tttcaggctc atgttgtaac aacttctgca aaggggttca tatttatcct tttcacccag
    15361 caccaccacc cgggattcat ctgatttccg gaatgaatac tgcgcgaggt tcccgcatcg
    15421 catacatatt gcatgcagtt ttgtaacata ttccgcgatt gcaaggagtg ccggcatcgg
    15481 accaaaggga tttcccatga aatccatatc gaggcctgcc acaataaccc tctttccatt
    15541 gttggctaat gtattgcata catcgacaat tgacatatca aaaaactggg cctcatcgat
    15601 cccgatcaca tcggcattac ctgacagcag gagtatcgaa gaagcattgt cggtcagggt
    15661 cgataaaata gatctgtcat catgtgaaac aaccctggta tctgaatacc ttttgtcaat
    15721 tgaaggtttg aatatttcaa cccgtaatcc ggcaaactgc gcccgacgga gccgcctgat
    15781 cagttcctcg gtctttcccg agaacattga accggtaatc acctcaacag acccccgcct
    15841 gtctttcatc ctgactgaat tttcaaggaa ttccataatc gtttacctgt ttccggaagc
    15901 taaatgtaac tcaattgcgt taagacttat ggaataatac ttacttttgc aaagtaaaaa
    15961 aattggttca ttaaaataac tgttgaaagc atggatttaa agaccacctt tgatataatt
    16021 atcaaggatc tgaatgatat acgggagatt attgacgatc tgaagcaata tcagggggtt
    16081 ccggcaatag aaattgagct tgcaaagtca aaatgcagaa gtgtaagcga aattatctct
    16141 tttctgagga acagtgatct ttcgcatgtc gggcggaaac aggacatatc tgaaaaaagg
    16201 gaagaatcca ggtcacatca gaaagaccct gttctgtctg caaagaaacc agttgaaatt
    16261 caggtggaaa aacctgcttt tcatgaaccc gtgaaggtcg acgccccgca ggataaacct
    16321 atatcccctg ttgtagtcac tgaacgtccg gaaccggttc cggttcccaa agctgcggaa
    16381 tatacaatta ttgccgacca gtttgccaac agaccggaaa gcttccatga aaagctcggt
    16441 ggtatgaagc cggaagatga tgttctggaa ataataaaga ccaaacctgt ttcaagcatt
    16501 tcagaagcta tagggataaa tgacaaatac ctttttataa aagaaatatt caacggcgac
    16561 caggaaacct ataaccgggt actttcagaa cttgaatcag ccgggagttt cgatgatgca
    16621 aaatccatta taatggatcg tgccgggatt aacaaggaaa atgaggcatt caggatgttc
    16681 atcagcatat tgaaacgcaa gttcccttcc aatgagtaag ctcatcctgg tccctactcc
    16741 gatcggtaat ctcagggata taacatacag ggctgtcgaa atcctgaagg gagttgatct
    16801 tatactggca gaagatacga ggcagacagg aaaactttta tccttctaca atatcaggac
    16861 accgattcaa tcgcatcata tgttcaacga gcacaggagc gtggaggcaa taagcagcag
    16921 gatattgtcg ggtacgactg ttgcccttgt cagtgacgcc ggtactcccg ggatctccga
    16981 ccccggattt ctgcttgtga gaaagtgcat tgaaaaaggg atcggggtgg aatcccttcc
    17041 cggccccacg gctcttatcc cggcgcttct gaattccggg ctcccctccg acaggttctg
    17101 ttttgagggc tttcttccgc ccaggaaggg aaggaacaaa aagcttgaag tgctggcccg
    17161 ggaaccaaga acaatggtgt tttatgaatc cccgttccgc ctcataaaaa cactggacga
    17221 cctggcatta ttctgcggtc ctgatcgcga agcttcggtg tcacgtgaat taacaaagat
    17281 ccatgaagaa accataagag gttcattatc attccttgct gaacatttca gggagaagac
    17341 accaaaaggg gaaatagtta tagttgttgc cggcaggaag ctatgaaaca aaagatacta
    17401 tgcctgacgc tgaaagccag gaagatattt atcgccgcca ttctgactgg cgttccattt
    17461 atttcaatcc atacccagat actgaagggc aggatcactg acagatcagg ggagccggtc
    17521 cagtatgcaa ccgtatattt ccaggaactg aaacacggga ccacatcaaa tcccaggggc
    17581 gattatgaga tcagactccc tgacggcaag tattcagtta tataccagag tcttggtttt
    17641 gagcctttta tttcagaggt aaccataaag ggagatacca ttgtaaggaa cgttgtgctg
    17701 gtggaacagt attatcagat acctgaagtg ctgatcactg aaaaaggcga ggatccggcc
    17761 tattatatca tgagaagagt aataggtctt gccccgtatc atctgaatca cgtaagttat
    17821 tacaaagcag aagtatacct taaggggaac ctgcatataa aaaagatacc aagggtcgtt
    17881 cagagatcca tgaggatggg atcacgcaac agggcatcag aacctcctga catgcaagtg
    17941 aagatcaggg agggtgattc cttctttctc gagtcattca atgagattga tttcaaggca
    18001 cccgataatt atgttcaaag agttatatct ttcaaatcaa ccttcccggg tcaggacgac
    18061 aatataacgc cgatggatta catcaaggca agtttctatc agccggtcat cgcccgtatt
    18121 gcgatttcgc ccctgtcgcc atcggcattc tcatattaca ggttcaaata cctgggatct
    18181 tctcttcagg gcgatcacag cgtgagcaaa atcgaagtga caccgaaaca ccgaagcccg
    18241 cagcttttcc agggaaccct gttcgtcgtt gatgatgtct ggtgccttca cagcgtggat
    18301 cttaccaatg aaaacatggc aggaaagatt catataaggg aattgcacat tccagtgcag
    18361 gatgagatct ggatgccggt gagtcactac tttgaaatga acatggggat aatgggcttc
    18421 aaagccgatg ttaattacac aagctccgtc aagtacatga ccgtggaggc aaacactgcc
    18481 cttaaaaagc ctgatccgct gacagggaca tattcagacc aggtcactgc cgccgataca
    18541 ctgatgacga aatcacgggg aaaaatcgaa gagatcctcc ggaaggaaaa aatgtcgaat
    18601 cgggatatga tccggcttgc tgacctgatg aacagggaat cttctgcaag tttgagggat
    18661 tcacttccaa agagtctgga aataaaggat aagacacaat atatcattga aaaggatgcc
    18721 ggtaagaagg acagctcatt ctgggccggc atcaggccca ttcccctgtc ggaagctgaa
    18781 ctgaagacgt tgggcacaaa acccggcacc ggcagtctga taaaaactgc cggcgattca
    18841 tccgcaatgg cggaacaaag aaaaagaacc ccttttatac gcaccctgag gcatattgca
    18901 tttggtcata cctgggcaga taccaccggc tggcggatct ccggtggcga cctggtaaat
    18961 acaagatgct cgggttttaa cactgtcgac gggtttatcc ttggaaccga tatcagaata
    19021 acgaaagaat tcaggaataa atggagtttt tcactttatc ccgaaatcag atacgcattc
    19081 agcagggaga aactgatggg aagagccaac atgaatatta attcgggagg gatgaaacct
    19141 gtgagatttt ttatgcagtc gggaattaca agcagggaca tcagcaccgg agggggaatt
    19201 ccacctttgc tgaacagtgc aacatcgctg ctgcttaaaa aaaattacct gaagctttat
    19261 gaaagccgtt atcttaatct cggccttggt ttcgaaataa aaaacggatt caatgttgag
    19321 ctgaagtctg gcatcgataa ccggcatata cttgaaaaca ctaccgactt ctcacttttc
    19381 aggttgtcgc gcgaatatac cgacaatatt cccgacaaca ggtatcttaa tcttgaagaa
    19441 aactctccgt cccgataccc gttcaacgat caaaaacaga tcagcttttc tgcaaagctc
    19501 acagttgtac cataccagaa atacaggatt atcaggggaa ccaaggttcc tgaaggctcg
    19561 caatggccca cattcaggtt cttctgggaa cattttatga attctgtttc gccgggagaa
    19621 gcgaaatatg cccatttcaa tatgttgaaa ttcgaagcat cccagaaact tagtcccgga
    19681 gcttacaggg aatttaaatg gttgttcagg acaggcggtt acactgacaa caggggaata
    19741 tccttttttg acttcttcca ttttaacaat cagtcatttc ccctgctgct atatgaccat
    19801 gatgacgcgt tcatgatacc aaggtattat acccttagca caccggaatt tttcgccgaa
    19861 gctcacttga aatatacttc gccctatctt ctgatgaaat atttgcctgt attcaacagg
    19921 acgctcatga gagaaaacct ggtattttct tatctcggat cgcgctacag caacagctat
    19981 actgaaatcg gctacagcgt gagcgagatc ttcctcattg ccgagctggg agtttatgcc
    20041 ggattcgaga atctgagcta caaatttacc gggataaaga tatccttcag gttttaattg
    20101 atatgagaag gaggacggcc gggtaactgt catcttgagt acatcctccc gtcccgggaa
    20161 aagattttca ccttcctaca tgaaaggagg ttcattcaga aggtcggttt catgttcaaa
    20221 atcattgaag gatcggttcc tgctgatgtc atgattcatc tttgatccga ttgtaaagct
    20281 ttgtgatccc tctctgatat cggtgatcag atcggtttca aaatcctcga tatcaacaaa
    20341 ctgtgccttc tcttctcgga atctgagcct tacatcgtcc accgggccgt tccggttttt
    20401 tgccagtatg atctctgcta ttcccctggt tgatgaaccg tcttcgaaag tgagcattcc
    20461 gaatttctcc tgcctgtgta taaaaaccac catgtcggca tcctgttcga tggcacccga
    20521 ttcacgcaga tcggaaagaa ggggtcgctt cgagccgccc cttatctcca ccgaacggtt
    20581 aagctgtgaa agggcaagaa taggcacatt gagttctttt gcaatacttt tcaacgacct
    20641 tgaaatactg ctgacttcct gttccctgct tcctgcatta tccggccccg acataagctg
    20701 caggtaatca acaataacaa tatcaagctt ctgctgtgcc ataagcctcc ggcattttgc
    20761 cctgagttcg aaaactgaaa tggctggagt atcatctata aaaacggggg cctgaaccag
    20821 tttctttatc ctgctgtcga gctgcttcca ttcctcttcc gaaaggcgac cattcttgat
    20881 cttctctccc ggaatatctg tttcagcaat tatcagcctg ttaaccagtt gtatcgatga
    20941 catctcacag gaaaagattg ctactttctt accatggtca attgccatgt ttctcgccat
    21001 cgacagggca aatgcagttt ttcccattga gggacgggcg gcaatgataa taagttcgga
    21061 tttctgccag ccggaagtaa gcctgtcaag cttggtaaac cctgaaggta tccctaccag
    21121 ggcatcttcc cgctttccgg cttcttcaat ctcccggatc gcctctttga tcaccaggtt
    21181 gatcggcgat acttcacgct tgatgttgcc ttctgctatc tggaaaaggg cattttccga
    21241 ataatcaagg agatcggtta catcatacga atcatcgtat gagcgtacct gaatctctgt
    21301 cgacacccgg ataagttcgc gctggatgta tttttgtgcc acgatccttg catgataatc
    21361 cacatgtgcg gctgaaacaa ccttcgatgt gagctgtgtg agatatacgg gcccgcccac
    21421 gctgtccagc tcgtttgtgg cccttaattc ctccgtaact gtgtaaaggt cgacaggatg
    21481 ttccctcatg gacaggtctg aaactgcctt gaaaatcttc tggtgagcct ccttataaaa
    21541 actttccggt ttcagaatat caagtatggt tataactgcc tccttttcaa gcatgattgc
    21601 gccaagaaca gcctcttcca tatctattgc ctggggagga acccttccat aatcgggtaa
    21661 tgagatgatt gcaggtccgg atacggtctt cgattttgcc atttatgcta ttgtgctgat
    21721 taataaatac atggatggct ttaacttatt gttttcaggg tagtccaaag gtaaatataa
    21781 ctatgcatcc tagcctgccg aacgtttttt tcttacaaac attttcctgt taaaaacttg
    21841 ttgaaaagca aaaaaagtaa ggttctgcac attattccaa accatatggt atttttgtag
    21901 taaatatcct gaaatggtca tttttcctaa agcaaaaatc aatctgggac taaggatcac
    21961 tgcccggagg ccggatggtt ttcatgatat tgaaaccatt ttttatcccg ttggtctttg
    22021 cgatatcctg gagatgaccg taaacgatga taaaaagggg aaggatattt tctcgctcag
    22081 cgggttgggt ctcgacggag atcaggacgg gaacctggta atcaaagctg tcaggaagtt
    22141 aagggaagct tacgaccttc ctccgcttaa gatacatctt cacaagatca ttcccccggg
    22201 cacaggcctt ggaggaggat cctccgatgc agcaggtgcg ctcaggacaa tgaacagggt
    22261 attcgaactt gccttaagtg cacaggaact taaaaccatc gcactggatc tgggtagtga
    22321 ctgtccgttc tttatcgatt gtcagccggc atttgccact ggaaggggag agaacctgcg
    22381 acaggtggat gccgtccttg aagggctcta cattgtactg gttaatcccg gaattccgat
    22441 cagtaccagg gaagcatacc tgaaatgcag tccttcaaag accggagtta acctttccga
    22501 actgattcat tacccggttg cagaatggaa atcacatata ataaacgatt ttgagaagac
    22561 tgtctttgag caatatccgc aaatcggcat aatcaaaaga gttctgtata ataacggggc
    22621 gctttacagt tcaatgtcag gaagcggatc agctgtctac ggcatttatg cggacaaacc
    22681 cgagatcccg gaaatactca gggattatgt aatctataaa ggcatcctgt agaagtcacc
    22741 tgatttgctg tcggacaatg acgctaatcc gttctgaacg gaaaaaatgt ttccttttat
    22801 ttcaattcaa tcaatacact gccttttgca accttatcac ctttttttac tcctattttt
    22861 tttattgtgc catcagccgg gcacttcagt ctgttctgca tcttcatggc ttcaagtatg
    22921 atcaggtcgt cgcctttttt aactttttgt cctcttttaa cgaggatatc aacaatggtt
    22981 ccgggaataa agctgagtat tgtcccggga tccgaaggct gatatggttt tctgttcagg
    23041 aacctggtgc taagcctggt tttgtagtct gttgagttta tattgagtat tcccagtctt
    23101 ttatccttat ccgttgtttg tttcatattt attaccggta tttctttcaa aaaggcggca
    23161 ggccgtgttt tttatcagga agagtcacag tcttgttttc cgaaacctgc attgcatgca
    23221 aaagaaaacg ccttgtttca gagggctcaa ttacagcatc aatgtatccc ctcgaggctg
    23281 caacatacgg gttggcaaac ttggccttgt attcctcgac tttttgtttt cttgttttgt
    23341 cgggatcatc cgacgacatg atctcttttc tgaaaataat attggctgca ccctcagggc
    23401 ccataacagc tatttcagca gtgggccatg cgaatacaaa gtcggccctg aggtgccggg
    23461 aactcatggc aatgtacccc cctccgtaag ctttcctcag tatcaccgta agtttgggga
    23521 cagtagcttc actatatgca tacaatactt ttgctccgtg acggatcaca ccagcatgtt
    23581 cctgatctat ccccggaaga tatccgggaa gatcgaccag cgtaatgatt ggaatattga
    23641 aggcatcaca gtagcggatg aaccttgccg ccttgtcaga tgaatcaaca tccaggaccc
    23701 cggccatctc aagcggctga tttgccacaa atcctgttgt cctgcccccc atccgaccga
    23761 aaccgatcac aatgttcctt gcataatgtt ccatgatttc aaagaagtcg gatttgtcga
    23821 caatcgccat gattacatcc ctgacgtcat atggcagggt tggatcatcc ggcactattt
    23881 tatcaagatg atattcattg aggggttcat gagtttcaga aacttttgcc ctcaggctgt
    23941 tgtttcgggg aatgaaggat ataaggcgtt ttatctggcc aaagcattca tcttcacttt
    24001 tggcaaaaaa atgtgcattg cctgtaactt cgctatgcac ccttgcccct cccagctctt
    24061 ccatggatat ctcttcacca agaactgttt tgatcacttc aggtccggta ataaacattt
    24121 tggaaatatt gtcaactacg aaaacaaaat ccgtcagagc cggggaatac actgcaccgc
    24181 ccgcacaagg gcccaggatc accgagatct gcggtattac accgcttgaa atagtattcc
    24241 ggaaaaaaat ctcgccatat cctgccaggg aatttactcc ctcctggatc ctggccccgc
    24301 ccgaatcatt gatcccgata agtggcatcc tcattttcag cgcatgatcc attatcttgg
    24361 tgatcttacg tgaatgcatc agtccgaggg accctccggc caccgtgaaa tcctgtgcaa
    24421 aaactgcaac aggagccccg taaatagtcc ccgagccgat tatcacgcca tcgctgggaa
    24481 gggatttgtt ttccatgtca aagtcgcgcg catcatgctc cacgaaaaga tcatattcat
    24541 tgaaggagtt tttgtccagt agagccatga tccgttctct ggccgtacgc ttccccatgg
    24601 ctttctgctt ttcaatagcc tgtacacctc ctcccaggag tatcttactg cgcttctcct
    24661 gcaattccct gatcttcttt tcaactgcca tagaaataat aattttttaa aactgcctgc
    24721 tgctgctttc taataataag caaagtacaa aaataacaga ttactgccgg gttttgcaat
    24781 aatgattggt cgttatttcc tcacaatcat caatattttc agattcttac aacatgtatg
    24841 caaagtgccg gcatggatga caatcaatca gtcttatttg ggagccaccc tgtacagaaa
    24901 gacagcgata catgcatata aaatgttcgg gatccacatg gcaagcatag gccccaggtt
    24961 gccttttatc gaaaactgag atgcaaactg cataaaaagg atatatgaaa agctcagccc
    25021 aaggcctatt ccgatattct ttcctatccc tcccctgacc ttgcgcgagg aaagcgaaac
    25081 gccaatcagt gtcaggatga acaccgcaaa aggattggca atccttttat gcttttcgat
    25141 gagaaacact ttcagttcat cagaaccctg aagctgcagc tgatcaatgt aatcattcag
    25201 ttccctgtag gtcatggtgc cttcaaatcc tggcaccctt gaaaaatcgt taggttttat
    25261 tgtcagtgtg gtgtcaatcc tgtcaccttt aataatcgtt tcctcgtttc cgtcaatttt
    25321 ccttatatag tatgtcatta cgctccattt gtttgttgcc gtatcgtatc tgaccatatt
    25381 tccgttcagc ttggattgga gcctgccgga atcatcaaac tgttcaaggg tgaaattgcg
    25441 gcctgtgagg cttatcgggt taaacgactc aaggtatata tatacattcc tgaatacctg
    25501 cctgtgaaga ttcattacgg gcattcttct ggcagcagaa ggcctgtaat agcggtcttc
    25561 gaaaaccatg cgttttgaat ttgcctgagg aataacaaaa ttggtaagga ggaaaatgaa
    25621 aatggcaatt atgagtgatg ccaggaaata aggatacatc attcttctga aactcatccc
    25681 gctgtttagg atagcaatga tttcagtatt tgccgccatt tttgaagtga agaaaataac
    25741 cgcaataaat acgaaaagcg gagcgaagag agtggagaaa tatggaatat agttaagata
    25801 ataatcgaaa ataatggcac gcacaggcgc ctctctttcc atgaaattat cgatcttttc
    25861 tgcaaaatca aacactacgg ctattgtaat tataaggaag aggcaaaaaa ggaaggttcc
    25921 caggaacttc ctgaaaatat agcgatcaat gatttttata ctgaaaaatt ctataaccgc
    25981 gctgaaagct tttttatcat tgcgtttttc cattctctga aattaccttc ctgaattctt
    26041 attctggctt cctttaccag tttcatgtaa aaggcaagat tgtgaatact tgcgatctgg
    26101 gctccaagaa tttctcctga aataatgaga tggcgcagat aggccttcga ataatgacgg
    26161 cttatcctgc tgggaccgaa actgtcgatg gggttgaaat catccatcca tttcctgttc
    26221 cgtatgttaa tcacaccctc cccggtgaaa agcattccgt tgcggccatt acgggtgggc
    26281 atcacgcagt caaacatatc gacccccctt tcaattgcct caagaatgtt tgcaggagtg
    26341 cccacaccca tcagatatct gggtttatcc tccggaagga tcccattgac aatttccaga
    26401 atcctgtaca tctcttcagc cggttctcct actgaaagtc ctcctattgc gtttcccgcc
    26461 agtgataatg atgctatctt ttcggcagaa agctttctca gatcgtcaaa gatgctcccc
    26521 tgcacaatcg ggaaaagata ctgatcgaat ccccacaggg gttcagactg attgtaacgc
    26581 aatactgccc ggtcgagcca tttgtgagta agctccattg atttccttgc gtaccggtaa
    26641 tcgcagggaa acggggtgca ctcatcgagg gccatcatta tatccgcacc tataatccgt
    26701 tgaatatcaa tcgtattttc gggtgtgaaa acatgctttg acccatcaat atgcgacctg
    26761 aaaatcgcac cttcctcact aagcttcctg ttccttgcaa gcgaaaagat ctggtaaccg
    26821 ccgctgtcag tcaggatagg cttttcccat cccatgaact catgaagtcc gcggcagctt
    26881 tttaaaatat ccgtcccggg cctgagataa agatgatagg tatttcccag gattatttct
    26941 gctcctatct cctcaaccag gtcctgctgc aatattcctt ttacggtccc ggctgttccc
    27001 accggcataa acacgggtgt ctgaacatct ccgcgggaag ttctgaggat tcctgcgcgc
    27061 gcatttgtac ccctgtccct gctctgtatt tcaaacaata ccatcctgct gttttggaac
    27121 gacaaaggta attcattagt tatggcctgt aaaattaata ttaacaattt ttgaaactgg
    27181 tcaatgtttt tagctaaatt aagaggctca ctttttgcat tcagattatc ttgctaatat
    27241 tgtcccaata tttgaaaacc agatgctgat cagggaaaac ttaatcttca acatcgcttt
    27301 tactgttttt cttctatctg ccgctatcca gttgttttat tatttctttt ttttcctcag
    27361 tgcaggactg tccgggaaag ctgctcaatc ccatgattac aaacccgtat ctgtcatcat
    27421 ctgcgccagg aacgaagaaa aaaatctgcg attatatctc acatcggtac ttgaacagga
    27481 ttatcccgat ttcgaagtta ttgtggtaaa tgactgcagt gaagataatt cctacaacgt
    27541 cctgggagaa ttgcttccgg cttatcccca tctcaggatc agcaatatag ccagagaccc
    27601 caaatttacc cacaacaaga aatttgcaca gttcatcggc ataaaagctg caaaaaatga
    27661 gcttcttcta tttactgatg ccgattgcca tcccgaatct gacaactgga tcaggggcat
    27721 ggttgatggt tttgatgaaa aaactgattt tgtactcggt tatgggggct attcatcgga
    27781 aaaagggcta ctcaataagt acatcaggtt tgataccatg atgattgccc tccagtattt
    27841 cggaatggcc ctccgcggta tgccatacat gggagtgggc cggaacctgg cttacagacg
    27901 atcggtgttt tttaaaaaca agggcttcgg acaacattcc cacctgctgt caggtgacga
    27961 tgaccttttt gtaaacaaac atgccaacag gcagaatact gccgttgaaa ccagccgcga
    28021 aacacatacc agatccgttc cttcccggac atttgccgaa ttcttccgcc agaaaagaag
    28081 acacttaaca acggctcctc attacaggct ccgcgacaga atcattctca ttgctgaacc
    28141 tgcaacccgg ttgctttttt acatttcatt cataatcctc tccttttccc gcttctggcc
    28201 ctatgcggtt gcaatctttg cgggaaggat gattgtgcaa tcagtaacat ttaaatttgc
    28261 tgtgaagaag tttaatgaac caggtatttt caaatattcc ttacttttcg atatattttc
    28321 tccatttctg aacttagcat tttatttaag caattacctg cagagccccg gaaaaaacca
    28381 atggaaatag cttcaaacga attatcggaa aaggcaaaaa atgatctcct tttcgtcaat
    28441 atggcaagaa acggcgatga aaaggctttt gcaaccctga tgaaccgcta ccgcgattca
    28501 atctactata tgctcctgaa gatggttaac aatatccctg atgctgaaga tcttactatt
    28561 gaagctttcg gcaaggcatt tcgtaacctg gattcctata ctcccgaatt tgccttcagc
    28621 acatggctgt ttaagatagc caccaacaac tgcatcgatt tcataagaag gaagcatgct
    28681 tttccgtccg ccattcaaca gtcacaggat gatcttgaat caatcaccag gaacatacaa
    28741 tcggatcttc ccgacccgga agagactctg ataaacgatc agaagatagc cgtcctgcgc
    28801 agcgtcgtca accagctgaa acccaggtac aggcagctta ttgaactaag atactacaag
    28861 gaatattcat acgaggaaat tgcttccgaa ctgaatcttc cgctgggaac tgtcaaagcc
    28921 cagcttttca gggccaaaaa cctgctttac aacattttca aaaaaaccgg caacagatag
    28981 tgatccggga tataatccga tatttcccct ttgtcacagc agtccagaca gacaggttcg
    29041 gccagcttcg ggaaatatat gaatactgga acagcaggat aaacctggta tcacgcaagg
    29101 atatggataa tttctatatc catcatgttc tgcattccct ttcaatagcg aaaataatcc
    29161 ggttcctgga tggaaccagt atcctggatg taggaaccgg aggaggattt cctgggatcc
    29221 cactggcaat aatgttcccc ggttgtaaat tctttttgct tgattccatt ggaaaaaaga
    29281 tcaaagccgt ggctggggtg gctgaaacac tcggcctgga aaatgttacc gtcgtaagga
    29341 aacgtgccga agacgaaacg gaacgatttg attttgcagt gagcagggca ttaatgccaa
    29401 ttccgggatt tgttaacctt gtaaccaaga atatcacccc ggccggcaga aacacccttg
    29461 aaaacggtat aatctatctt aagggaggtg atatagatga agagatcaga ccattcagga
    29521 ataaggttaa gatttggaac ataagtaact atttcgacga gccatatttt gagacgaaaa
    29581 aggtcattta tatcccggta ttttaatgca ataatactcc cgtcgttgca tgattaaaaa
    29641 aaatgtctaa atttgcgctt tcaaattact gtttaaaatt taacaataag agtcatgaaa
    29701 aggacgtatc agccttccag gagaaaaaga gcaaacaagc atggtttcag ggaaagaatg
    29761 tccacaccaa atggaaggag ggtattggct gcccgcaggg ctaaaggcag aaaaaaacta
    29821 accgtttctt cagaactgaa attgaaataa cccggaataa aatcgcttaa taaaagaggt
    29881 gtccgtcggg gacgcctttt ttatttttct tccgggccgt cattaaaaat gaatatatga
    29941 tcatgatgtt accgcagccg aaaaagaaat tgctaatttt gcaccatccg aaaagcatat
    30001 gataaatcct gaaaaatata taagtgatcc agcactcctg aaaattgcag aaaagattac
    30061 agatgaaaaa cgcatcactc ccgaagaagg acttacgctt tatcaaaatg ccggcttgcc
    30121 cctgctgggt cttcttgccg gaatagtcag gagacgaata aatggtgaaa atgccttttt
    30181 catcaggaat ttccatatag aacccacaaa cagatgcatt tataattgca ggttctgctc
    30241 ctaccgcaag ccgccaaaac atcctgagag ctgggattta agccatgaac agatgcttga
    30301 gacagtaaaa aggtttgacg gcagacagat tgaggaagtt catattgtag gaggcgtaca
    30361 tcctgaacat gatcttcatt actacggaga gctgatcagg caaatcaaac aataccgccc
    30421 ggggcttcat attaaagcat tctcggccat tgaactggat tatatgataa ggaaaagcgg
    30481 atattccctt gaagaaggac tcggaatcct taaagattac gggttggatt caataccggg
    30541 aggcggagcc gaaatatttg acgaacaaat cagaaagatc atctgtcccg aaaaagcctc
    30601 ctccgacatg tggctcagga ttcatgaaac tgcccacctg gcagggatcc catcaaacgc
    30661 gacaatgctt tacgggcaca tggaaacata ttcccatcgt atcgaccata tggaacggct
    30721 tcgtctatta caggataaaa cacagggatt caatgcattc attcccttga aatacaaaag
    30781 ggagaataac cctatgtcgc acctgggcga ggtgagcatg attgaagatc tgagaaatta
    30841 tgccgtttcc aggatctacc tcgacaatat tccgcacctg aaagcctact ggcccatgac
    30901 aggaaaagaa gttgcgcagc tcagcctttc attcggca
//
LOCUS       DAOOYG010000021        29423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1350_length_29423_cov_6.827840, whole genome shotgun sequence.
ACCESSION   DAOOYG010000021 DAOOYG010000000
VERSION     DAOOYG010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29423
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1350_length_29423_cov_6.827840"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PK288_03445"
     CDS             complement(<1..580)
                     /locus_tag="PK288_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18005.1"
                     /translation="MKAFHCISGVVLLTLASCSSGLYVGTEYDDLYFQSADKPVVRIH
                     SATNQAIVERDLRSDNYYDNIYAADTLVSGEFSNAANYDNAVINNNYYFNDYSYSGRL
                     NRFYGNYFYPYWRDPFYLMPSFGYSYYGLPYYSPYYYDPFYYDPFYYGYGGFYSSFYF
                     GYGGLYNRFYYPYYYGYYSPFWGGCFWYDKDYG"
     gene            1207..2682
                     /gene="proS"
                     /locus_tag="PK288_03450"
     CDS             1207..2682
                     /gene="proS"
                     /locus_tag="PK288_03450"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPM18006.1"
                     /translation="MAKFLTNRDENYAQWYNDLVIKADLAENSAVRGCMVIKPYGYAI
                     WEKIQAELDRMFKATGHVNAYFPLFIPKSFFSREAAHVEGFAKECAVVTHYRLKTDET
                     GKGVVVDPEAKLEEELIIRPTSETIIWNSYKNWIQSYRDLPILINQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHATREEAVEETERMINVYSEFAENFMALPVYKGYKTASERFA
                     GALDTYTIEALMQDGKSLQAGTSHFLGQNFAKAFDVQFTDRNGRLEYVWATSWGLSTR
                     MVGALIMAHSDNHGLILPPKLAPLQVVIVPIYKSADELSSISEKAEALKAELERSGLS
                     VKYDNRETNKPGWKFADYELKGVPVRIAIGPRDLKNGTAEIARRDTLAKETVPMESII
                     NRIHELLNQIQKNIFERALRFRNDNTFRLDKWSDFVEVLNSSGGFISAHWDETAETEE
                     KIKEETKATIRCIPFDSAAEEGKCIYSGKPSGRRVLFARSY"
     gene            2829..3539
                     /locus_tag="PK288_03455"
     CDS             2829..3539
                     /locus_tag="PK288_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18007.1"
                     /translation="MPETEDKKQIIVGTLKQKSLLKQKVYDITLEWFSVIKDILKNLA
                     KDVNANLGNIDPRIRLEYTDRSNFDAQLKVAGDILLFSMHSNVFQFDRDHPAWKTPYI
                     QKNRLNAYSGIINIYNFLFDSFRYSRLDDLGYLIARIFINHENQYFVEGKRQMGMLFS
                     NYGNESVSRDSLQTIISTAIQYSLEFDLLVPPYDTVKIATVGQAEAKIQHSRVITGKR
                     LGFQFNSDDVLINKTHET"
     gene            3529..5478
                     /locus_tag="PK288_03460"
     CDS             3529..5478
                     /locus_tag="PK288_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18008.1"
                     /translation="MKHDIPKTGYFRIFHISILVPVFLFLPLQALPQTIQYPDSIMQN
                     PPGVPVMNTGHIRLLLLDPGPRLPDTLIPLSGLRYDRTMIFLDSLKSRAAKTLVTRKL
                     YDFLVIQPHSPSGRTISSTSEFNYVIHSGKRIRSIKIRELSVFGSDINNPEVFAPNGL
                     ERLLNSTHINTSERIIRKNLLFTPGDTISPIQLSDNERLLRDLPFIADARIMVIPVSD
                     NEADIMVLTKDIYSLGASANIGSLKKGTVSVFEKNIFGIGHEFRVEVPYDSRYAESPG
                     IGIQYLANNINRSFINLGVYYYDGPGKKTYGFDLGRRFVSSTTKYAGGISLRQMSTTE
                     DLDSLTIPAPVKYNLQDYWLSRSFLIDPENVVRLVIGGRYTNNNVFDRPFILPDSYRN
                     LQKYRIILGSMMLSVQKYYKTNLIYSYGRTEDVPYGVLADITAGWETSEFKNRLYMGT
                     GFSFGQSVNKLGYFYGSAGFSVFLNNSQTEQGMVMLRTRYISNLLYAGRSRVRNFVNI
                     DYTRGFDRYYDEFLVFNRENGFSGFRNDSIGGTQRLSFNLESVVFSPVSFYGFKFAFF
                     GFADFGFLFGTNETPASGDFLSAIGLGIRIRNENMVFNTFQMRLAFFPNPPDYSRLNN
                     LLITGEQLLRPRNFEPDIPSLLPYK"
     gene            5484..6821
                     /gene="tilS"
                     /locus_tag="PK288_03465"
     CDS             5484..6821
                     /gene="tilS"
                     /locus_tag="PK288_03465"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPM18009.1"
                     /translation="MNDMLADFLEYIEKNNLCRTDDRILLGVSGGIDSMVMTRLFITG
                     GFKTAMAHCNFCLRGTESDNDEVLVAKTAEAYNVPFFVKKFDTSGFARENGISVQMAA
                     RELRYQWFEEIRSAGNYDLIAVGHNLNDNIETLLINLVRGTGLKGLTGMKPISNRIIR
                     PLLFATRAQIEEYSKQEKIPFREDRSNLDIKYTRNKIRHVLIPLLKEINPGIETILND
                     TAERLSETSDIVSRYLAIVGKETAFPEKGIVQIKIKNLIPYIENRTILFELLKPYGIT
                     GPLITDLICLIRGTSGSRITTSSHSIIRNRDYLIVKPLVESHKTVWCEINNIEELSVS
                     AGIESAEIISMAPGFLIPDDRNIACLDFSKISFPLVIRNWKPGDSFVPLGMTHRKKLS
                     DYFTDRKFSIVKKEEILLLESEGRIAWIIGERIDDRFKITPATERILKVRAKK"
     gene            complement(6873..7868)
                     /locus_tag="PK288_03470"
     CDS             complement(6873..7868)
                     /locus_tag="PK288_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPM18010.1"
                     /translation="MNSFGEVFRVSIFGESHGNSIGAVIDGCPPGIPVTADDFVHDLS
                     RRRSGRTGTTKRHEPDKPEILSGIFNDHTTGSPITVITRNSDKISSDYDDFRNIPRPG
                     HADFTAGIKYSGFSDMRGSGHFSGRITWGLVAAGVIAKKILQPAEISARLISAGGNPD
                     IESAIEKAVLENDSIGGIIECKVTNPRKAIGEPFFYSVESAISHIVFSIPAIKGVEFG
                     SGFSAASMKGSVHNDPIIDGTGRTLTNNAGGINGGITNGNEILFRVAVKPTSSTGAEQ
                     RTYDFSTGQLTALKVKGRHDTCIALRIPVIVEAAAAIALADLLLLDRGIHGSRSL"
     gene            complement(7888..8925)
                     /gene="aroB"
                     /locus_tag="PK288_03475"
     CDS             complement(7888..8925)
                     /gene="aroB"
                     /locus_tag="PK288_03475"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPM18011.1"
                     /translation="MEKITVNIPGSVSEIFVGREWETAGKLIPQSGVLIITDQNVHSI
                     YGNRFPEFPVLIVQPGEQSKKLEVIGQLAERLLDAGIDRSGFLLGIGGGVVCDITGFL
                     ASVYMRGIRFGYVSTTLLSQVDASTGGKNGVNLGDSKNILGCFRQPEFVICDPVMLYT
                     LSDEEYFSGLAELIKTGIIGDRLLFEKIEQNYNAIIGRDPELLSSLVSMAVNFKAAVV
                     TEDERESGLRRILNFGHTFGHAIELSSSLAHGYAVASGMELAARFSCNKGLISVTDLE
                     RITGMLKSFNLLRDYHIPGDQVGRLILHDKKKAGGDIYFVLTEGIGKAVVEKMPAGTI
                     VDFYLKYKAGK"
     gene            9069..9710
                     /gene="nth"
                     /locus_tag="PK288_03480"
     CDS             9069..9710
                     /gene="nth"
                     /locus_tag="PK288_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPM18012.1"
                     /translation="MTMKERYRRTIEYFNATIPVAETELVYSDPYSLIVAVILSAQCT
                     DKRVNMITPPLLEKYPDIKSMAEARPEDIFEFIKSCSYPNSKTKYLSGMAKMLINEFN
                     GLVPSEPELLRRLPGVGRKTAHVIASVAFRKPVMAVDTHVMRVSKRIGLVINAKTPLE
                     VEKQLTANIPAHMLYKAHHWLILHGRYVCKARNPLCSECGLREFCKYYHSEKT"
     gene            9783..11912
                     /locus_tag="PK288_03485"
     CDS             9783..11912
                     /locus_tag="PK288_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM18013.1"
                     /translation="MYLIDQMKSLMVDYREKYEEQEALNKKLSDELESLRGKNDRGKL
                     LLDNLLDSIPAAIVIVDLQGNISGINREFTSLFGYTADEARGMKLEDLIVPDDLLRDA
                     PGIHDLTIENNRIEKKIIRRRKNGKQINVSLIATSIIINGEIAAYLGIYRDISTDIKN
                     QLIQEILYNISTAALNQMDVKDIYPIIVHELNKIWDTTNFFIALYDKKTETLSMPFFA
                     DEKDSFDKVPARKTITRWVIGMKRSVLLKEEDLKNLENSGDIALVGTPCKIWMGVPLK
                     AGNEIIGIMALQDYHDENKFSQDDVNVLEFIANQIAIAIQRRTMLDNMIQASLKAEEA
                     ARSKQQFMSTMSHEIRTPLNEVIGIANLLMQGNPREDQMDFIRTLRFSGNHLLTLVND
                     VLDFNKMESGKIVFEQVQFNLGEFLQDIMRTYSFRSKAKNLDFDIVSRDNLPAEVIGD
                     PIRLNQILSNLLSNALKFTSSGSIHVILNELSRTGNNIRIEFRVQDTGLGIPKDRQKI
                     IFDSYTQASADTTRQYGGTGLGLAICKKLVELQGGTIDVESEPGAGSTFRFFLTFGIP
                     EKTETAGKTEKPESYSGLEGKRILMAEDNKVNFFVANKFLVSWGVQVTHAENGKIAVD
                     LLNREDFDLVIMDLHMPVMDGIEATRIIRESPDMRIRSIPVIALTAAIMSETHDKIED
                     LAINDYVLKPFKPRDLFDKIYKNIRVE"
     gene            complement(11926..13125)
                     /locus_tag="PK288_03490"
     CDS             complement(11926..13125)
                     /locus_tag="PK288_03490"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM18014.1"
                     /translation="MKKASIFILLFLPLFLLGQEKAYLLMKARALTESGKPDEAITLL
                     TGQGTEGLDVPVLLQRADAYISAGNYSGATDDFNSANKLSPGSGEYGLARIYGMKGDA
                     STAVYHLERCLGSPFKRSEKEIMLDQAFASVENKTEWRQFWKKEWYTQLEKGISEIEY
                     YVSTGNIDEAKNLYSGLAGQYSGKPALLYGNALINFSSSRYGEAVKILTSLLEEDRQN
                     DKYLRLLAKAQEASGNYAGASDTYTRLIHSGNSDPELLVSRSECYRKTGETGKAVDDI
                     EKYLEFYPSSTRALSIAGRLETARGDNLKALEYFNRNVRLHPSDPQCYIDRANSYFLS
                     KSWDWAIKDYSMSLDLQPDNSETWLNKGISLLNSGKVDDACFDFRKSLSLGNRKATEY
                     ISRYCIR"
     gene            complement(13145..15769)
                     /locus_tag="PK288_03495"
     CDS             complement(13145..15769)
                     /locus_tag="PK288_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM18015.1"
                     /translation="MKNLIRGIFVAAVLFITCSLKISATHNRAGEITYVQLSDLTFEI
                     TITTFTYTLSFADRQQLEVNWGDNSTSIAERNSILYLPNFYKRNIYTTTHTYPGPGVY
                     KIVVQDPNRNADVRNIPNSVNVVFSISTTLNINPAIGRNNTPVLYNPPYDKAARGYVF
                     IHNPGAYDSDGDSLSYKLTVCTREDGKPIENYTLPPATKFIRVDSISGDLIWNTPADT
                     GKYNVAMEISEWRRGKKIGMVVRDMQIEVYDTKNRPPVNGPLKNMCIEAGKPVDFLVT
                     ATDEDKDMISLKATSGVFGLANCPASFVKVDSVAGFASARFRWTPCFEAVRNQPYDVL
                     IKSDDYSDELKLSDIDNFQIKVLGPSPALLNGLPEGKFIRLRWDDYGSDYISGFNIYR
                     REGPSQFSPDSCTAGIPATTGFVKIGFAAGGSATSFVDTDGGHGLQYGKEYTYRIVAV
                     FPNGTESKASNEISSSLVSGIPVITNVSIRTTSVTNGSVYISWMKPGRLDTIPANGPY
                     EYLIYRAEGITGTDYQQIRSIRTATLNETSIIDTLINTRDKGYIYRIELYNNAPGNRF
                     LIGEPGYASSLFLTASPGDEKVRFVINRNVPWLNSRYDFYRLNSSTMRYDSIGSTNQL
                     TYTDLGLENGKEYCYRIRSVGGYVPDDLPKNLINYSQIVCATPEDNEPPCQPVITVTS
                     QCDSLYNTIRWRFDDPECIDDVAGYYIYFKPYYEGSLSKINTIDNSNTFVYKHYPGDV
                     VSGCYAVSAFDRVGNEGEKSYMVCIDTCDFYEIPNVFTPNGDNINDRLIARTSGQVEK
                     VDMKIFNRYGILVFQTDEPRINWDGTYKGKIVSPGVYFYQCEVFIRRITGLERSHLHG
                     FVHVITEKGAGKNVEILK"
     gene            complement(15884..17311)
                     /gene="pyk"
                     /locus_tag="PK288_03500"
     CDS             complement(15884..17311)
                     /gene="pyk"
                     /locus_tag="PK288_03500"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPM18016.1"
                     /translation="MNKRTKIVATISDKRCDPEFIAELYREGVNVVRINSAHLNLEGA
                     LKIIGNVKKVSDKIGILIDTKGPEIRTTHCDSPIELMKGGIILMMGDPDKNSSERAIY
                     VNHRNIAEDVPVGSSILIDDGDIELKVIKKDDDYLECRIENDGLLGSRKSVNIPGVKI
                     CLPSLTAKDRIFIRMAVEQGLDFIAHSFVRTARDILDVQAVLDEYESNIKIIAKIENH
                     EGVENFDEILEHAYGIMVARGDLAIEIPYEKIPAIQRMMISKCIQKRKPVIVATQMLH
                     SMITNPRPTRAEVSDIASAIYSQTDAIMLSGETAAGKYPVEAVKTMTRVAIEAENDKE
                     KFIDHPALNSSGNISHYLAKVAVKTSVRIDARVIIADTVRGRSIRDMAGYRGFKLILA
                     QCYLTSTMRQLALSYGVYASYQERKKSIDDFLKIALTELTGRHDLKSDDVIVVLAGNF
                     SGSSGFSFIEVGTVDYLKDRVDITD"
     gene            complement(17308..17748)
                     /gene="aroQ"
                     /locus_tag="PK288_03505"
     CDS             complement(17308..17748)
                     /gene="aroQ"
                     /locus_tag="PK288_03505"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPM18017.1"
                     /translation="MKITIINGPNLNLLGRREPEKYGTVTFESFLLSLKERYPLINFD
                     YFQSNIEGELVGRIQEAGFSADGIILNPGGYTHTSVAIGDAVSSVAAPVVEVHITNIQ
                     AREEFRHESLVGRFCAGSILGFGLDGYRLAVEALIAKAKGKQNL"
     gene            18198..19298
                     /locus_tag="PK288_03510"
     CDS             18198..19298
                     /locus_tag="PK288_03510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPM18018.1"
                     /translation="MKQLIIAAVMMITGLSVHGRDQETKNGNNIRFYDQNHFLIEGIA
                     FADSVRESPYDRFPLSMKSKVREPVWSLSRNSAGVSIRFISNTTAIRVKWELLEDLRM
                     NHMAETGIKGIDLYFMGKAGWQYVNTARPEGKSNDFLLISNMIPERREYRMYLPLYDG
                     VTRIEVGIDSLAYISKPEKQKVRPIVFYGTSITQGGCASRPGMVHTSIISRKLGIECM
                     NFGFSGNGRMEKPVVDLIAGIDALFYVIECSENMTPEEIKANSGPLLEIIRQKHPITP
                     VLFVNNANYEISSLSESAMKYIIAKNKMFREVFMDLIEAGNKNIYLIDKEKSLGHDHE
                     ATVDGEHLTDLGFMRYAEFLLGRFRELGLPAE"
     gene            complement(19381..20184)
                     /locus_tag="PK288_03515"
     CDS             complement(19381..20184)
                     /locus_tag="PK288_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HPM18019.1"
                     /translation="MKQTLIDCLKMSGGLLLKKLGRELEVSLKENQSSIVTQADFKSE
                     KLIIENIRKRYPYHNIISEESGFIDSGSDFTWVVDPLDGTSNFASALPWFGVLITLFE
                     ADFPVMGGAYLPASDTLYLAEKGKGLYRNGKMLLMKKQHELRNALFAFSVDYTDDEKL
                     FSNCIGIYKKVLKTTRSIRSTNSLVDFLSVAEGKFGGCINLFTKIWDISGLGLIISEA
                     GGVVKNIDGNDLQFKLGSGSCENNYAIMAGSPAIVEEIKKHILNGSIPV"
     gene            20421..21320
                     /locus_tag="PK288_03520"
     CDS             20421..21320
                     /locus_tag="PK288_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HPM18020.1"
                     /translation="MGLVWKEALRNFSIYLRLEKSMSENSIEAYLGDIRKLEVFFTGE
                     GGAIEPADVTHNGLKKFLNHISEGGISPRSQARLISGIRAFYRFLLMDGEISENPSSL
                     LESPRTGLRLPEVLTVGEIDRMVGIIDLSKPEGHRNKAIIETIYGCGLRVSELVNLRL
                     TDIHRNEGFVKITGKGNKQRLVPIGHRTLREIDLYKIDRNRLPVIHDENIVFLNRRGR
                     KLTRSMIFAITRDLAERAGIKKNISPHTLRHSFATHMIEAGADLRAVQEMLGHESIIT
                     TEIYTHIDRSFLRDTLIMFHPRA"
     gene            21415..24141
                     /gene="ppdK"
                     /locus_tag="PK288_03525"
     CDS             21415..24141
                     /gene="ppdK"
                     /locus_tag="PK288_03525"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPM18021.1"
                     /translation="MSKIKRVYSFGRKTAEGKADMKNLLGGKGANLAEMSLLGLPVPA
                     GFTITTEACNEYYSNNCTLPEGLMAEVWEAMKVTEETMGMKYGDPENALLVSCRSGAR
                     SSMPGMMDTVLNIGLCTATIPGFLKKTNNQRFVWDSYRRLIMMYADVVMEKAEGIEPG
                     EGKGIRKQLDEMLDEFKHKKGYQSDTDITADELKELSEKFRERVKQVLGKPFPDDARE
                     QLEGSIKAVFKSWNGKKAVSYRRIEGIPDDWGTAVNVQAMVFGNMGDHSATGVAFTRN
                     PATGENLFYGEWLVNAQGEDVVAGIRTPSPLNNETKNEQNRHLQSMEEAMPGTYRELC
                     QIREILEKSFRDMLDIEFTIQEGTLYMLQCRAGKRTGTAALNMAMDMLRENLIDEKTA
                     VLRVDPVQLDELLHPICDPEAEKKVRPVVKGLPAGPGAAVGKIVFSAEDAVVWARKGN
                     KVILVREETNPEDVEGMRAAEGILTARGGMTSHAALVARGWGKCCIVGAGSLHVDAAA
                     KKAKVNGTDIVLKEGDVITLNGTKGNVYLGELDLMDATENPQFKSFMTMVDKYRKMGV
                     RTNAETVEDAKIARDFGAEGIGLFRTEHMFYGKNSEQPLFLLRKMILSANEAERRSAL
                     NELFIYIKEDMKETLEAMDGLPVTFRLLDPPLHEFVPQGAEKQAELAKALGIKESEVV
                     KRSEALHESNPMMGHRGVRLGITYPEITETQVRAMFEASAELIRAKKHPKPELMVPVT
                     CDVSELDVTKKIVDAVYAEVLKKTGLEKIDYKYGTMIEIPRATLLSDRMAKSAEFFSF
                     GTNDLTQMTYGFSRDDIGGFLHDYLDKGMLSADPFQTIDLDGVGQLIKMSVEKGRATR
                     PDLKVGICGEQGGDPASVEFCFKTGLTYVSCSPFRVPIARLAAAQASIRYGK"
     gene            24507..26126
                     /gene="pckA"
                     /locus_tag="PK288_03530"
     CDS             24507..26126
                     /gene="pckA"
                     /locus_tag="PK288_03530"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HPM18022.1"
                     /translation="MTEKELAEYGIMSPLEIIYNPSYDKLYEEETRPDLKGYEKGTVT
                     TTGAVAVDTGVFTGRSPSDKYVVLDDTTKNTVWWKSEINKISDNKPITQEIWEHCRNL
                     AAGQLSGKRVFVVDCFCGTNKDTRLSVRFVMEIAWQAHFVKNMFIRPDTGELNNFVPE
                     FVVLNASRVINPDWKKQGLNSENFVFFNLTSKMAVIGGTWYGGEMKKGIFSVMNYYLP
                     LKGIASMHCSANVGKKGDVAIFFGLSGTGKTTLSADPNRALIGDDEHGWDEEGIFNFE
                     GGCYAKCINLSKKNEPDIYNAIKRDALLENLVILPDGTIDFNDDSKTENTRVSYPIYH
                     IQNIVKPVSRAGHASKVIFLTADTFGVLPAVSRLTEEQTEYYFLSGYTAKVAGTERGI
                     LEPVPSFSACFGAAFLMLDPIIYAKELTKKMKEHGAEAWLVNTGWISGSYNTASRIDI
                     PVTRKIINSILDNSISKYPFTSLPVFNLNIPSRIDDIDPNLLDPQKAWDSAVKWRIAA
                     TDLALKFINNFNKFSGNDETMSLVAHGPRVI"
     gene            26138..26782
                     /gene="plsY"
                     /locus_tag="PK288_03535"
     CDS             26138..26782
                     /gene="plsY"
                     /locus_tag="PK288_03535"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPM18023.1"
                     /translation="MHVLKYTGVFILSYLIGSAATAVWAGRIFHKTDVRKHGSGNAGA
                     TNVIRVLGWKTGIPVLLIDIFKGYLAASLPVLFKLAGPGTAQLINLQILTGLLAVCGH
                     IFPVYAGFRGGKGVAAIFGVLLAIHPLLTLSCIGVFLLVFLATGYVSLGSLSAGVAFP
                     VFLFLFFDTPSVIFKVFSVVIAIMLIITHRKNISRLLKGEESKLLRKNTGTGKN"
     gene            complement(26789..27730)
                     /locus_tag="PK288_03540"
     CDS             complement(26789..27730)
                     /locus_tag="PK288_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPM18024.1"
                     /translation="MKYFGLQSQIRKNNVNSVLLLIMFPLVFYALTWLFFFFMSMVSG
                     NEQVSLFSINHSFLGTVPWITAGVAIWFVIAWFSHTAIINSATNSRPLERKQNKRVYN
                     LVENLCISSGMKMPKVNIIEDDSLNAFASGVNPSTYTVSLSRGIINKLEDDELEAVIA
                     HELTHIRNRDVRLLIISIVFVGIFGFIAEMLFRSLRFGRSGRGRKEGAGILIALVLAL
                     IGYLLAMLFRFALSRKREYLADAGSAELTRRPLSLASALRKISEDPVIEAVKRKDVAQ
                     MFIENPGLKEKESTFSFSGLFATHPPIKKRIALLEQY"
     gene            complement(27731..28294)
                     /locus_tag="PK288_03545"
     CDS             complement(27731..28294)
                     /locus_tag="PK288_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPM18025.1"
                     /translation="MGVLLVILGIIVAFILMAISLYNRLVRLRNSREQAFADVDVQLR
                     QRHDLIPQLVEAVKGYMVHERGVLTEITEARANAMKASTINDKIAAEARLSSALDGLR
                     VSVEAYPDLKASQNFMDLQNEISDVENKIAAARRFFNSATKELNVATEVFPSNIVATM
                     FNFKREPMFDLGDKRTVIEEPPQIKFQ"
     gene            28424..29002
                     /locus_tag="PK288_03550"
     CDS             28424..29002
                     /locus_tag="PK288_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPM18026.1"
                     /translation="MEKSLKGTKTEQNLLKAFAGESQAKNRYEFAAKVAKEEGYEQIA
                     GIFLETALQEQQHAKRFFNFLEGGMVEITACYPAGKTGTTKDNLNAAAEGEHEEWSEL
                     YPAFAEVAEEEGFKNIATAFRAIAAAEKGHEERYRKLLENLMKNKVFERDDKVFWYCR
                     KCGYIHFGTKPLKNCPACLHPMGYFEVKADNY"
     gene            29006..>29423
                     /locus_tag="PK288_03555"
     CDS             29006..>29423
                     /locus_tag="PK288_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPM18027.1"
                     /translation="MHDGKEMLGLILRRQSERKYSNKPVEPDRLERIIEAGRMAPSAC
                     NAQPWKFVVATDAEVIAGLAKAASAKLLGMNGFVGQANVIVVIVREKPNITSRVGGVV
                     KSKDYSSFDVGIAVNSMILQAAVEGIGSCIIGWFDEK"
BASE COUNT         8486 a         6633 c         6652 g         7652 t
ORIGIN      
        1 tcccgtaatc tttatcgtac cagaaacaac caccccaaaa aggtgagtaa tatccgtaat
       61 aatacgggta ataaaacctg ttatataacc ccccgtatcc gaaatagaaa ctgctgtaaa
      121 atccgccgta accatagtag aacggatcgt agtaaaaggg atcgtaataa tacggactat
      181 agtatggcag gccgtaatag ctgtagccga atgaaggcat caggtagaag ggatctctcc
      241 agtaaggata gaagtaattc ccgtaaaacc tgtttaacct gcccgaatat gaataatcat
      301 taaagtaata attattattg attactgcat tatcgtaatt ggctgcattg gaaaactccc
      361 ctgaaacgag tgtatctgcc gcataaatat tgtcgtaata attatccgac ctgagatccc
      421 tttcaactat tgcctggttt gttgcagagt gtatgcgaac aacaggtttg tcggcacttt
      481 gaaagtaaag atcatcgtat tcagttccca cataaagacc tgaggaacag gaggccagtg
      541 tgagaagaac aactccggat atgcagtgaa atgctttcat ctttttcctt tttaatcatt
      601 gtcattcatc tgtccgtaaa caaatactgt accaaattat ttgtttctca gcaggatctt
      661 gtcaggtaag gcaaatttaa tacttttcac gaagttacaa tttatttaca atcaaaaccc
      721 gccgatggta aaaaactcta ttctgcctca cggaatgctc caattgcctt atcagccaac
      781 cagattagcg gtatctgcaa ccggatgatg cagggcctaa tacatggcag gtttataagg
      841 cttcatcgct tcatgccata ttcatacggg ttatccggat gcccggtata cgtaatagta
      901 atatcaacga agcagctttt gcttttattg ttcgtgccgc ttgtatacaa caaacgtatc
      961 tgctgataat cagtcattta ctatcatttt gaaaaatctg ttcattaagc ccggtcatcc
     1021 gtggcaccgg gtacctgtaa ccgccgctgc cggtactgtc tggccaggac tcggatgtgc
     1081 cgctaaatga tgcatcagag ataaccttaa ttaacaggtc tcatggtgta tccctggttt
     1141 gttaattatc aagttttata tctattttta cggacttaat ttccaaagac aaatattaac
     1201 cgaacaatgg cgaaatttct tacaaacagg gatgaaaatt atgcacaatg gtacaatgat
     1261 ctggttataa aggctgatct ggctgaaaat tcagcagtca ggggatgcat ggtaataaaa
     1321 ccatacgggt atgccatatg ggaaaagatc caggccgagc tcgacaggat gttcaaggca
     1381 acgggtcatg tcaatgcgta tttcccgctg ttcattccaa aatccttttt cagcagggaa
     1441 gctgcgcatg ttgaaggatt cgctaaggaa tgtgcggtgg taactcatta ccgcctgaaa
     1501 actgacgaga ccggcaaagg ggtagtggta gatccggagg caaaacttga ggaagaactg
     1561 atcatccggc cgacctctga aacaataatc tggaactcat acaagaactg gatacaatcg
     1621 tatcgtgatc tgcctattct gatcaatcag tgggccaatg tggtaagatg ggagatgcgg
     1681 acaaggcttt ttctcagaac tgctgaattt ctctggcagg agggtcatac agcccacgcc
     1741 acccgggaag aagctgttga ggaaactgaa aggatgataa atgtctattc ggaatttgca
     1801 gagaatttta tggcccttcc tgtgtataaa ggatataaaa ctgccagtga aaggtttgcg
     1861 ggagcgcttg acacctacac cattgaggct ttaatgcagg atggaaaatc actgcaggcc
     1921 ggaaccagtc attttctcgg ccagaatttt gcaaaggcat ttgatgttca gttcactgac
     1981 aggaacggcc gacttgaata cgtctgggcc acatcgtggg gactgtcgac aaggatggtc
     2041 ggtgcactga taatggcaca ctctgacaat cacgggctga tactgcctcc taaactggct
     2101 ccccttcagg ttgtaattgt tccaatttac aaatcggccg atgaactcag cagcatcagt
     2161 gaaaaagcgg aagcattgaa ggcagaactt gaaagatcgg gattatcagt gaaatatgac
     2221 aacagggaaa caaataaacc gggatggaaa tttgccgatt atgaactgaa aggcgtgcct
     2281 gtaagaattg ccataggccc aagggatctt aagaacggaa ctgcagaaat agccaggagg
     2341 gatactctgg ccaaggagac cgttcccatg gaaagtatca taaaccggat acatgaactg
     2401 ttgaaccaga tccagaaaaa tatctttgaa agggccctca ggttcaggaa tgacaacaca
     2461 ttcaggctcg acaaatggag cgacttcgtt gaagtgctca acagcagcgg gggattcatt
     2521 tccgcacact gggacgaaac agctgaaaca gaagaaaaaa tcaaggaaga aacaaaggca
     2581 accatccgat gcataccgtt tgattctgca gctgaagaag ggaaatgcat ctattcggga
     2641 aaaccttccg gaagaagagt tctttttgca agatcatact gaccggattt cctcgccaga
     2701 ctcgtgctgc caaggctgac ttcatgcggc cgtcccggtc ttcatcctga cataccacaa
     2761 aaatagtgga tatcggttta tattaaattt actaaattag atgccagtta atactatata
     2821 cattcatcat gcctgaaact gaagataaaa agcagattat cgtcgggaca ctgaagcaaa
     2881 aatccctgct gaaacaaaaa gtatatgaca ttaccctgga gtggttcagc gtgatcaagg
     2941 atatacttaa aaacctggca aaggacgtga atgcgaatct tgggaatata gatccaagaa
     3001 taaggctgga gtataccgat cgcagcaatt ttgatgcaca gcttaaggta gcaggtgaca
     3061 ttctcctttt cagcatgcac tcgaatgtat ttcagttcga cagggatcat cctgcctgga
     3121 aaacacctta tatacaaaag aacaggctga atgcctattc cggaataatt aacatctaca
     3181 acttcctttt tgattctttc aggtacagca ggctggatga tcttggatat cttattgcca
     3241 ggatatttat aaaccatgaa aatcaatact ttgtggaagg caagcgtcag atgggaatgc
     3301 tgttttcaaa ttacgggaat gaatctgtca gcagggactc attacagacg ataatctcaa
     3361 ctgctattca atattcactc gaatttgatc tgctggtacc cccttacgac actgtcaaga
     3421 tagctacagt gggacaggca gaggccaaaa tccagcattc ccgggtaatc actggcaaac
     3481 gtcttggatt ccagtttaat tcagatgacg ttttgattaa taaaactcat gaaacatgat
     3541 atcccaaaaa cgggttattt ccggatattc catatttcaa ttcttgttcc ggtatttctg
     3601 tttctccctc tgcaggcact accgcaaaca attcagtatc ctgattcaat aatgcaaaat
     3661 cctcccgggg taccggtaat gaataccggc catattaggc tattgctgct ggaccccgga
     3721 ccccggctgc ctgacactct catccccctg tcagggctta ggtatgaccg cacaatgatt
     3781 tttcttgact cacttaaaag ccgcgcagca aaaacgcttg taacccgcaa actgtacgat
     3841 tttttagtaa ttcagcctca ttctccttcc ggcaggacaa ttagcagtac cagcgaattt
     3901 aattatgtga tccattcagg aaaaagaatt aggagcataa agatcaggga actcagcgtg
     3961 ttcggatcag acatcaacaa ccccgaggtg ttcgcaccga acggtctgga gaggctgctc
     4021 aattccaccc acatcaacac cagtgaacgg ataataagaa aaaacctcct ttttactccg
     4081 ggcgacacga tctcacccat tcagctaagt gataatgagc ggctcctgag agacctgcca
     4141 tttattgccg atgccaggat catggtgatt ccggtgtctg acaatgaagc cgatataatg
     4201 gtcctgacga aggatatcta ttcacttgga gcatctgcca atatcgggag cttaaaaaag
     4261 ggaacggtat ctgtattcga aaagaatatt tttggaatag gtcatgaatt ccgcgtggag
     4321 gtcccatacg attccaggta tgccgaatct cccggtattg gaattcaata tcttgcaaac
     4381 aacataaaca ggtcgttcat aaacctcgga gtatattatt atgacggacc agggaagaaa
     4441 acatacggtt tcgacctggg cagaagattt gtaagctcaa ccaccaaata tgcaggagga
     4501 atctcactca ggcagatgtc aacaacagag gatcttgact cactgaccat acctgcgccg
     4561 gtgaaataca atctccagga ttactggctg tccagatcgt tcctgattga tccggagaat
     4621 gttgtacgcc tggtaatcgg aggaagatat accaataaca atgttttcga caggccgttt
     4681 atcctccccg attcgtacag gaatcttcag aagtacagga taatcctcgg ttcaatgatg
     4741 ctttccgtgc agaaatatta caagacaaat cttatataca gctatggaag aactgaggat
     4801 gtgccctatg gagtactggc cgacatcacg gcaggatggg aaacaagcga attcaagaac
     4861 cggctttata tgggaacagg cttttcattc ggccagtcag taaataagct gggatatttt
     4921 tatggttctg ccggattctc ggtcttcctc aataacagcc agactgaaca gggaatggta
     4981 atgctgagaa caagatacat ttcaaacctg ttatatgcag gacgatctcg tgtaaggaac
     5041 tttgtgaaca tcgattacac ccggggattc gacaggtatt acgatgaatt cctggttttt
     5101 aaccgtgaaa acggattctc gggtttcaga aatgactcaa tcggaggaac ccagaggctg
     5161 tcgttcaacc tcgagtcagt ggtgttcagt cctgtcagct tttatggttt caagttcgcg
     5221 ttttttggct tcgctgattt cggattcctt tttggcacaa atgagactcc ggccagcggt
     5281 gatttccttt cagccattgg cctgggtata agaataagaa atgagaatat ggtattcaat
     5341 acattccaga tgcgactggc tttctttcct aatcctcccg actattcgag gcttaataac
     5401 ctgctaataa caggagaaca gttgctccgg cccaggaatt ttgaaccgga tataccgtcc
     5461 ctgctgccat acaaataggt taaatgaacg acatgcttgc ggattttctt gaatatattg
     5521 aaaaaaacaa cttatgcagg accgatgaca ggatcctcct tggggtgagc ggaggcatcg
     5581 attcgatggt aatgacccgt ctttttatca ccggaggttt caaaacggcc atggcccatt
     5641 gtaatttttg cctgagaggc acggaatcag acaacgatga ggtactggtg gcaaaaacag
     5701 ctgaagccta taatgtgcct ttttttgtga agaaatttga cacttcagga tttgctaggg
     5761 aaaatggtat ttcggttcag atggcggccc gcgaattacg ctatcaatgg tttgaagaaa
     5821 tcaggtcagc cggtaattat gatctcattg cagtcggcca caaccttaat gacaacatcg
     5881 aaactcttct gataaacctt gtaagaggga cagggctaaa gggacttacc gggatgaagc
     5941 ccataagcaa caggattatc cggcccctcc tttttgcaac acgtgcacag attgaagaat
     6001 acagcaaaca ggaaaagatc ccgttcaggg aagacagatc aaaccttgac ataaaatata
     6061 ccaggaacaa gataagacat gttctcatcc cgctgctgaa ggagatcaac ccggggattg
     6121 agacaatcct caacgacacg gctgaaaggc tttccgaaac aagtgacatt gtcagtcgtt
     6181 acctggcaat cgtgggaaaa gaaactgctt ttccggaaaa ggggatcgtg caaataaaaa
     6241 tcaaaaatct catcccgtat attgaaaaca gaaccatctt gttcgaattg ctgaaacctt
     6301 atggaatcac cggcccgctg ataaccgatc ttatttgttt gatacggggg accagcggca
     6361 gcagaattac aacaagttca cattcaataa tcagaaacag ggactacctg attgtgaaac
     6421 cgctggtgga aagccataag accgtctggt gtgaaataaa taacattgaa gagctttcag
     6481 tttcggcggg gatcgaatca gctgaaataa tctctatggc acccggcttt cttattcctg
     6541 atgacaggaa tatcgcctgc cttgactttt caaaaatcag ctttcccctg gttatccgga
     6601 actggaaacc gggcgatagt tttgtacccc tggggatgac acacaggaaa aaactcagcg
     6661 attacttcac cgacaggaaa ttctcaatcg ttaagaaaga ggagattctg cttcttgaat
     6721 cagaaggtag aatagcatgg atcatcgggg aaaggatcga tgacagattc aaaatcactc
     6781 ctgcaactga aaggattctg aaagtcaggg ccaaaaaatg attcccccgg aaagggattc
     6841 cattcagtga ccttttaaat agtcagattg ttttaaagac tccttgaacc atgaattccc
     6901 ctgtcgagga gcagcaggtc agccagggcg attgcagcgg cagcttcaac aatcaccggt
     6961 atccgcagcg cgatgcatgt atcatgccgc cctttgactt taagagccgt aagttgaccg
     7021 gtgctgaaat catatgttct ctgctccgcg cccgttgatg aagtcggctt aactgccaca
     7081 cggaacaaaa tctcatttcc gttggtaatc cctccgttaa tgccgccggc attattggtg
     7141 agcgttcgtc ctgtcccgtc aataatcgga tcattatgca ccgagccttt cattgatgca
     7201 gcagaaaatc ctgaaccaaa ttcaacaccc ttgattgcag gaattgaaaa aacaatatgg
     7261 ctaatggccg attcaaccga atagaaaaaa ggttctccga ttgccttgcg tggattcgtg
     7321 accttgcatt cgattattcc tcctatggaa tcattctcca aaacagcttt ttcaatagcc
     7381 gactcaatat cagggttgcc gcctgccgaa attagtctgg cggatatttc ggcaggttgc
     7441 agaatctttt ttgcaatcac gcccgcagca acgagacccc atgttatccg tccggaaaaa
     7501 tgaccgctcc cgcgcatgtc ggaaaatccc gagtatttta tacctgcggt aaagtctgca
     7561 tgacccggcc gcgggatgtt tctgaaatca tcataatctg aggatatctt gtcactgttc
     7621 ctggtaataa cggttatcgg cgatccggtg gtgtggtcat tgaaaatacc gctgagtatt
     7681 tcaggcttat caggttcatg acgctttgtg gtgcccgtcc ttccgctccg cctgcgcgaa
     7741 agatcatgaa caaaatcatc agcagtgacg ggaataccgg gcgggcagcc atcaatgaca
     7801 gcaccaatcg aattgccatg cgattcacca aaaatagaca ccctgaatac ttctccaaat
     7861 gagttcataa ccgatccctt tttaatatta cttaccagcc ttgtatttca aataaaaatc
     7921 aactattgtc cctgccggca ttttttcaac aacagctttt cctatacctt cggtaagcac
     7981 gaaataaata tctccgcctg cctttttctt atcgtgcagt ataagcctcc cgacctgatc
     8041 gccgggtata tgatagtccc tgagaagatt gaacgatttc agcattcccg tgatcctttc
     8101 aagatccgtc actgatatca ggcctttatt gcaggaaaac cttgccgcca gttccatgcc
     8161 tgaagccact gcatagccgt gagccagcga actgctgagc tcgatggcat ggccgaatgt
     8221 atggccaaaa ttaagtatgc gccttaagcc cgattccctt tcatcctcgg tgaccacagc
     8281 agccttaaaa ttaacggcca ttgaaaccag cgacgacagc aactcaggat ccctgccgat
     8341 tatggcatta taattttgtt caatcttctc aaataataac ctgtccccta taatacccgt
     8401 ttttatcagt tccgcaaggc ccgaaaaata ttcctcatcg gagagggtat acagcataac
     8461 cggatcgcat attacaaatt caggctgcct gaaacatcca agtatgtttt ttgaatcgcc
     8521 gagatttacg ccgtttttgc cgcccgtgct ggcgtcgacc tgcgaaagaa gggttgtgga
     8581 aacatagcca aaccgtattc cgcgcatata gaccgatgca aggaatccgg ttatgtcaca
     8641 aaccacaccg cctcctatcc cgagcagaaa acccgagcgg tcaatcccgg cgtccagaag
     8701 cctttctgca agttgtccta tcacctcaag ttttttgctc tgttcacctg gctgaactat
     8761 cagcacggga aactcaggga acctgttccc atatattgaa tgtacattct gatcggtgat
     8821 tatcaatact ccgctttgcg gaataagctt cccagctgtt tcccattccc tgccaacaaa
     8881 gatctctgaa accgatccgg ggatatttac ggtaattttt tccaaattaa aagaatttcc
     8941 ggccggtaaa gataacaaat ccggagatca aactgtttat cgggatattt caaaataaat
     9001 tgaatctccg gtcagatcat gatccccaaa atacaaaaag tattatcttt catggctgtt
     9061 ttgctatcat gaccatgaaa gaaagataca ggaggacaat cgaatatttc aatgctacta
     9121 tacctgttgc agaaaccgaa cttgtttaca gcgatcctta cagcctgatt gtggcagtga
     9181 ttctgagtgc gcaatgcacc gacaaaaggg tcaacatgat cactcccccc ttacttgaaa
     9241 aataccccga cattaaatcg atggctgaag ccagaccgga agatattttt gaattcataa
     9301 aatcatgctc ctaccccaac agcaagacga agtatctttc tggtatggcc aaaatgctga
     9361 tcaatgagtt taacgggctc gttccttcag aaccggagct tctccggcga ctgcccggtg
     9421 taggcaggaa aactgcacat gtcattgcat cggtggcatt cagaaaaccg gtcatggcag
     9481 tcgatacgca tgttatgaga gtttcaaaaa ggattggact agtcataaat gcaaaaacac
     9541 cccttgaagt agaaaaacaa ctgacggcaa atatccctgc ccatatgctg tataaggcgc
     9601 atcactggct catcctgcac ggccggtatg tctgcaaggc aaggaatccg ctatgcagcg
     9661 aatgcggcct gagggaattc tgcaaatatt atcattccga aaaaacataa cacctttatc
     9721 cggcagataa tttttcaggg aaattggagt tttatcctta ccaacgcaaa cagtttgcca
     9781 ttatgtattt gattgatcag atgaaaagcc taatggtcga ttacagggaa aaatatgaag
     9841 aacaagaagc tctgaacaaa aaacttagtg atgaactgga atcactcagg ggaaaaaacg
     9901 atcggggaaa gcttcttctt gacaacctcc tggattcaat acctgccgcc attgtcattg
     9961 tggatctcca gggcaacata tcaggtataa acagggagtt cacgagcctg tttggctata
    10021 cggccgatga ggcccgggga atgaagcttg aagatcttat tgttccggat gaccttttaa
    10081 gggatgcccc cgggatccac gaccttacaa ttgaaaacaa caggattgaa aagaaaataa
    10141 taagacgcag gaaaaacgga aaacagatca acgtttctct tattgcaacc agcattatca
    10201 taaacggcga gattgcagca tatctgggaa tctaccggga tatttccacc gacattaaaa
    10261 atcagcttat ccaggagatc ctttacaata tttcaactgc agctctcaat cagatggatg
    10321 ttaaggatat atatccaata atcgtacatg aattaaacaa aatctgggat actaccaatt
    10381 ttttcattgc tctttatgat aaaaaaaccg aaaccctgtc gatgccgttt tttgcagatg
    10441 aaaaagacag tttcgacaag gttccggcta gaaagacaat caccaggtgg gtcattggca
    10501 tgaaaagatc cgttttgcta aaagaagagg acctgaagaa cctggagaat tctggcgaca
    10561 ttgcgctggt agggactccc tgcaagatct ggatgggagt tccgttgaaa gcaggcaatg
    10621 aaatcatagg gataatggcg ctccaggatt atcacgacga aaacaaattc tcgcaggatg
    10681 atgtaaatgt cctcgagttt attgcgaacc agattgccat tgcaattcaa aggagaacaa
    10741 tgctggataa tatgattcag gccagcctta aggccgagga ggcagcccgg tcaaagcaac
    10801 agttcatgtc aaccatgagc catgaaatca ggactccgct taatgaggta attggcattg
    10861 caaatctcct gatgcaggga aatccccgcg aggaccagat ggattttata aggacactca
    10921 ggttctcggg caatcacctt ctcaccctgg tcaatgacgt ccttgatttc aacaagatgg
    10981 aatcgggcaa gatcgtgttt gaacaagtcc agttcaatct cggggaattc ctgcaggata
    11041 taatgcgaac ctattctttc cgctccaagg caaaaaatct tgattttgat atcgtcagca
    11101 gggataatct ccctgcagag gtaataggtg acccgataag gctgaaccag atcttgtcga
    11161 atcttctgtc gaatgcccta aaattcacaa gttcgggaag tatccatgtg atcctcaacg
    11221 agttgtcaag aaccggcaat aatatcagga ttgaattcag ggttcaggat acaggactgg
    11281 gcattccaaa agaccggcag aaaattatct tcgacagcta tacccaggct tcagccgata
    11341 caacgcggca atatggagga acaggtttgg gactggccat ctgcaaaaag cttgtcgaac
    11401 tccagggagg cactattgac gtggaaagcg agcctggtgc cgggtcaaca ttcagattct
    11461 tccttacttt cgggattcct gaaaagacag aaacggccgg gaaaactgaa aaaccggaat
    11521 catattcggg cctggaaggc aagagaatac tgatggctga agacaacaag gttaatttct
    11581 ttgtcgcaaa caaattcctg gtaagctggg gtgtgcaggt cacccatgca gaaaacggga
    11641 aaatagccgt ggatcttctg aacagggaag atttcgacct cgtaataatg gatcttcata
    11701 tgccggtcat ggacggtata gaagccacaa gaatcataag ggaatctccc gatatgagga
    11761 tcagatcgat tcccgtgatt gcccttactg cggccataat gtcagagaca catgataaaa
    11821 ttgaggacct tgccataaat gattatgtcc tgaaaccttt taaacccagg gatctttttg
    11881 acaaaatata taagaacatc agggtagaat aacatgatca tttgatcacc tgatgcaata
    11941 gcggctgatg tattcagtgg cttttctgtt tcccaggctc agtgattttc tgaaatcaaa
    12001 acatgcatca tcaactttcc cggagttgag aagtgaaatt cccttgttga gccatgtttc
    12061 agaattatcg ggctgaaggt cgagcgacat gctgtaatcc ttgatggccc agtcccatga
    12121 ttttgacaga aagtacgaat tagcccggtc tatataacac tgtggatcag aagggtgaag
    12181 cctgacgtta cggttgaagt attccagtgc cttcagatta tcacccctgg ccgtttcaag
    12241 tcgtccggcg atactcagtg ccctggtact ggaaggataa aactcaagat atttctctat
    12301 atcgtcaaca gcctttccgg tttcccctgt tttcctgtag cattccgacc tggataccag
    12361 aagttccgga tcggaatttc cggaatgtat cagccttgta tatgtatccg aagcaccggc
    12421 atagttgccc gatgcttcct gggcttttgc aagaagcctg agatacttat cattctgtcg
    12481 gtcctcctca agcagggagg ttaggatttt tacagcttca ccataccttg acgatgaaaa
    12541 atttatcaaa gcattcccgt aaagcagggc cggcttgcct gaatactgcc cggcaagccc
    12601 ggaatagagg ttctttgctt cgtctatgtt acctgttgaa acatagtact caatttccga
    12661 aattcctttt tccagctgtg tgtaccattc ctttttccag aactgccgcc attcagtctt
    12721 attctccacg gaagcgaatg cctgatcgag cataatttct ttttcgctcc ttttaaaagg
    12781 cgatcccagg catcgttcaa gatgatatac tgcagttgaa gcatcacctt tcatcccgta
    12841 tattcttgcc agaccgtatt cacctgatcc cgggctaagc ttattggccg aattaaaatc
    12901 atccgttgcg ccggaataat tgcccgcaga aatatatgca tcagcacgct gaagaagaac
    12961 agggacatcc aaaccttccg ttccctgtcc tgtaagcagg gtgattgcct cgtccggttt
    13021 tcccgattca gtcagggcac gggctttcat aagcaggtag gctttttcct gacccaggag
    13081 aaacaggggt aagaacagta aaataaagat tgatgccttt ttcattatgt tttgacccgg
    13141 tttttcattt taatatttca acattcttac cggccccttt ctcagttatt acatggacaa
    13201 aaccatggag atgactccgt tcaaggccgg ttatccgtcg tataaacacc tcgcactgat
    13261 aaaagtagac tccgggtgaa acaatctttc ccttgtatgt tccgtcccag ttaatccgcg
    13321 gctcatcggt ctggaaaacc agaattccat accggttgaa tatcttcatg tcaaccttct
    13381 caacctgtcc ggaagttctt gcaatcagcc tgtcgttaat gttatcccca ttgggagtga
    13441 acacattcgg aatttcatag aaatcgcatg tatcgatgca aaccatgtat gacttctctc
    13501 cctcgttccc gacccggtca aaggcggaca cagcgtaaca gcccgacacg acatcacccg
    13561 gataatgctt gtaaacaaag gtattactat tgtcgattgt gtttattttt gaaaggcttc
    13621 cctcgtaata tggtttaaaa taaatataat aaccggcaac gtcatcaatg cattccggat
    13681 catcaaatcg ccatctgatt gtattataaa ggctgtcgca ctggcttgtg acagtgatta
    13741 ccggctgaca gggaggctca ttatcctcag gtgtcgcaca tacgatctgc gaataattaa
    13801 taaggttctt cggcaaatca tcgggtacat atcccccaac cgatctgatc ctgtaacaat
    13861 actcttttcc gttctcaaga ccaagatcag tataggtaag ctgattggtg gaaccaatgg
    13921 aatcatacct cattgttgag ctgttaagcc tgtagaaatc atatcgtgag ttcagccacg
    13981 gaacattgcg gtttatgaca aacctcacct tctcatctcc cggactggcg gtcagaaaca
    14041 aggaggaggc ataacccggc tcgccgatca ggaatctgtt tccgggagca ttgttataca
    14101 attcaattct ataaatatac cctttgtccc tggtattgat gagcgtgtcg atgattgatg
    14161 tctcattcag ggttgcagtc cgtatggatc tgatctgctg gtaatcagtc cctgtaattc
    14221 cttccgccct gtaaatgagg tactcataag gtccgtttgc agggatcgta tcaagcctgc
    14281 caggcttcat ccagcttatg tacacagatc cgtttgttac cgaggtggtt ctgatactga
    14341 cgtttgttat tacaggtatg ccagatacaa gcgatgagga gatctcattg gaagccttac
    14401 tttccgtacc attgggaaac accgcaacga tcctgtaggt atattccttt ccatattgaa
    14461 gtccatgacc gccatctgta tcaacaaatg atgttgccga gcccccggca gcaaacccga
    14521 tcttaacaaa acctgtggtg gcaggaattc ctgctgtgca tgagtcgggg ctgaactgtg
    14581 aaggtccctc gcgccggtaa atattgaagc cggaaatata atcactgcca taatcatccc
    14641 atctcagacg gataaatttg ccttcgggca gtccattcag aagtgcaggt gatggtccca
    14701 gaacctttat ctgaaaatta tcgatgtccg aaagcttgag ctcgtcgctg taatcatccg
    14761 atttaataag gacatcatag ggctggtttc tcacagcttc aaagcatggt gtccaccgga
    14821 atcttgcgga tgcaaatccg gcaacagaat caaccttaac aaaacttgca ggacagttgg
    14881 ccagaccgaa aacaccagaa gtcgctttta atgatatcat atccttgtct tcatccgtgg
    14941 ctgttacgag aaaatcaacc ggttttcctg cctcgatgca catattttta agcggaccgt
    15001 tcaccggggg cctgtttttc gtgtcgtata cctcaatctg catatctctt acaaccattc
    15061 cgattttttt gccgcgccgc cattccgata tttccatcgc aacattgtat tttcccgtat
    15121 cggcaggggt gttccagata agatcgccgc taatggaatc aactcttatg aattttgtgg
    15181 cggggggcag ggtgtagttt tcaatcggct taccgtcctc cctggtacac actgtaagtt
    15241 tgtatgaaag gctgtcgccg tcggaatcat aggcaccggg attatgtata aacacgtaac
    15301 ccctggcagc tttgtcgtat ggcggattat aaaggactgg cgtgttgttc cgtcctattg
    15361 caggattaat gttaagcgtt gttgaaattg agaagacaac attcactgaa ttcggaatat
    15421 tcctgacatc ggcatttctg ttcggatcct gtaccacgat cttgtacacc ccgggacccg
    15481 gataagtatg tgtcgttgta taaatattcc tcttgtaaaa attggggaga taaagaatgc
    15541 tgttgcgttc agctattgaa gttgaattat cgccccaatt cacctcaagc tgctgacgat
    15601 cagcaaaact gagcgtgtat gtgaaagtgg ttatggttat ttcaaatgta agatcggaga
    15661 gctgaacata ggtaatctct cctgccctgt tgtgtgtggc actgatcttc aggctgcacg
    15721 tgataaacaa aacggctgca acaaaaattc ccctgataag attcttcacc cggataatta
    15781 ttttaatttc ttaatatatc agcctgaccc agctcctgcc ctggtaagaa taacatcgtc
    15841 cgtatcatca atctacctca aaaatcagac caagcatttt gattcagtct gtaatatcca
    15901 ccctgtcctt taaataatca acggttccaa cctcaatgaa agaaaatccg gaactgcctg
    15961 aaaaattccc tgcaagcaca acaattacgt catcactctt aagatcatgg cggccggtca
    16021 gctccgtaag cgcaatctta aggaaatcat ctattgattt tttcctttcc tgataacttg
    16081 cataaacacc gtatgaaagc gccagctgcc tcatggtact cgtcagataa cactgggcaa
    16141 ggatgagctt aaaccccctg tatccggcca tatcccggat tgaccttccc ctcactgtat
    16201 cggcaataat caccctggca tctatcctga ccgatgtttt aactgctacc tttgcaaggt
    16261 agtgcgatat atttcccgat gaattcaatg caggatggtc aatgaatttt tctttatcat
    16321 tctctgcctc aatggcaacc ctggtcattg tctttactgc ttcaacgggg tattttcctg
    16381 cagcggtttc accgctcagc atgatggcat cggtttgtga ataaatggca ctggcaatat
    16441 cgctcacttc agccctggtc ggtctgggat tcgtaatcat tgaatgcagc atctgtgtgg
    16501 cgacaataac cggttttcgc ttctgaatgc attttgaaat catcatgcgc tgaatggccg
    16561 gtatcttttc atatgggatt tcaatggcaa ggtcgcctct tgcaaccatt attccgtagg
    16621 catgttccag gatctcgtca aagttctcaa ctccttcgtg attctctatc ttcgcgatta
    16681 tcttgatgtt gctttcatac tcatccaaca ccgcctgaac atcaagtatg tcacgggcag
    16741 tccgtacaaa tgaatgggcg ataaaatcaa gcccctgttc aacagccatc ctgataaaga
    16801 tcctgtcttt cgctgtcagt gacggcaggc atattttcac ccccggaata ttgacactct
    16861 ttctcgaacc aagcaggccg tcattctcaa tacggcattc aagataatca tcatcctttt
    16921 ttatcacttt gagctctatg tccccgtcat caataagtat tgaagagccc acaggcacat
    16981 cctcggcaat atttctgtga tttacatata tagccctctc tgagctgttc ttgtccggat
    17041 cacccatcat gagaatgatc ccgcccttca tcagctcgat gggagaatca caatgcgtgg
    17101 ttcgtatctc gggacctttg gtatcgatca gaatgcctat tttgtcggag acttttttca
    17161 cgttgccaat gatcttcaat gctccctcaa ggttaagatg agctgaattg atcctgacta
    17221 cgttaactcc ttcccggtat agctctgcaa taaattccgg atcacaacgc ttgtcagaaa
    17281 tggttgcaac gatcttggtc ctcttgttca taaattctgt tttcctttgg ccttcgctat
    17341 aagtgcctcg accgcgagtc tgtaaccatc aagcccgaac cccaggatgc ttccggcaca
    17401 aaacctgcct acaagacttt catgacggaa ttcctcacgc gcctgaatat tcgtgatatg
    17461 cacttcaacg acaggtgcag ctaccgaaga aaccgcatcc ccgattgcaa ctgaagtgtg
    17521 agtgtaaccg cccggattga gaatgatgcc atcggctgag aatccggcct cctgtatcct
    17581 gccgacaagt tcaccttcga tatttgactg gaaataatcg aagttgatca gcggatatct
    17641 ctctttcagg gaaagcaaaa agctctcgaa ggtgacggta ccatattttt ccggctcccg
    17701 cctgccgaga agattcaggt tcgggccgtt tataattgtt attttcattg ctcgccagac
    17761 taaaccggtt caggaggtaa aaatagataa aaaatagcaa agtgcgtcaa taatagcggg
    17821 aggagccttt gatgatgcat aaatcctgca gaataactga aatcgcctta ttgataattt
    17881 taaaatagtt ttttgttact tttatgctat cagcagaaaa ctccggggat atccggctaa
    17941 tccaatcagt ctgaaatgac gggcatatac agtttaaata caaacagatg cgggaataat
    18001 atgtcaaatg accgtgtcag acatatatgt catttaactg caggaattgt cagatcttcc
    18061 ggcaaaactg caaagtgtca tttatcagta cgatttcatc gcaaattaca tttacccgtt
    18121 tcgggtacgg ttgcgtcatc cgaagttaat aaaaacctct gctcatctca gaactttaaa
    18181 atccaaacca gtaatttatg aaacaactga tcatcgcagc tgtaatgatg atcaccggct
    18241 tatctgtcca tggccgggat caggaaacta aaaacgggaa taacatcaga ttctacgatc
    18301 agaatcattt tctgatcgag gggattgcct ttgccgactc ggtaagggaa tccccgtatg
    18361 acaggtttcc gctttccatg aaaagcaaag tcagggaacc cgtatggtca ttatccagga
    18421 attctgccgg agtcagtata agattcattt cgaacacgac cgcgatccgg gtaaaatggg
    18481 aactcctgga agacctgagg atgaaccaca tggccgaaac gggtataaaa gggatcgatc
    18541 tttactttat gggaaaggcc ggctggcaat atgtcaatac tgcgcggccc gaaggtaaat
    18601 cgaatgattt tctccttatc agcaatatga tacccgaaag aagggaatac aggatgtatc
    18661 tcccccttta tgatggtgta accaggatag aagtagggat tgacagtctg gcatatatct
    18721 caaagcctga gaaacaaaag gtcagaccca tagtttttta tggaacaagc ataacccagg
    18781 gaggatgtgc ttcgcgtccg gggatggttc atacaagtat aatctcaaga aaacttggca
    18841 ttgagtgcat gaatttcggg ttcagtggca atggcaggat ggaaaagccg gtcgttgatc
    18901 tgatcgcagg gatcgatgcc ctgttctacg ttattgaatg ttcggagaat atgacgcctg
    18961 aggaaatcaa agcaaactca ggtcctcttc ttgaaattat caggcagaag catcctatta
    19021 cccctgttct tttcgtgaac aacgcgaact atgaaatctc atcactcagt gaatcggcca
    19081 tgaaatacat tattgccaag aataaaatgt tcagagaagt cttcatggat ctgatcgaag
    19141 ccggtaacaa gaatatttat cttatcgata aagaaaagtc gttgggccat gatcatgaag
    19201 caactgtgga cggcgagcat cttaccgacc ttggatttat gcgatatgct gaattcctcc
    19261 tggggagatt cagagaactt ggattgcctg ctgaatgaac gggagacggc agtaggatcc
    19321 gcattcagaa ccaatgtacc cttcatggaa tcaggcaaat cagggagggc aggcagaatc
    19381 tcataccggg atgctgccat taagtatatg tttttttatc tcttcaacta ttgcgggcga
    19441 accggccatt atggcataat tgttctcaca gctgcctgaa cccagtttga attgaagatc
    19501 attgccgtca atgtttttaa caacacctcc ggcttccgaa attatcaggc ccagtccgga
    19561 aatatcccag atcttcgtga acaggttaat gcagcctccg aatttccctt ctgccacact
    19621 cagaaaatca acaagtgaat tggtggaacg aatactcctg gtggttttta acactttctt
    19681 ataaatgccg atacagttgc tgaataattt ttcatcatca gtataatcaa cggaaaaagc
    19741 gaacagggca tttctcagtt catgctgttt tttcattaac agcatttttc cgttcctgta
    19801 aagtcctttt cctttctcgg cgaggtaaag ggtatctgat gcaggaagat aagcccctcc
    19861 cattacggga aaatccgcct caaacaaggt aatgagaacc ccgaaccatg gaagggcaga
    19921 ggcaaaattg gaagttccgt caagcgggtc cacaacccag gtaaaatcag accctgagtc
    19981 gatgaatccg ctctcctcgg agatgatatt gtggtaagga tatctctttc ttatgttttc
    20041 gataatcagc ttttcacttt taaaatcagc ctgtgtgact atactgctct ggttttcctt
    20101 taagctgacc tcaagctcac ggcccagctt ctttaacagc aacccgcccg acattttcag
    20161 gcaatcgatc agtgtttgtt tcattttatg gtgttttcct gaagttaaaa agaccgcctg
    20221 caatttcccg ggtgatggat gaacggtcat taatataata tttgaaaata ccaaatatag
    20281 ggaaatgccg gaatataagg ggcctgagtc cgaatgttat cagcgggccg catttcatta
    20341 catcctgaat tccgggctga tatttcccca aaaaatagtt agcttcgcat aggctgatcc
    20401 ggttaaaaac agagggcaga atgggtcttg tatggaaaga ggcactaaga aatttcagta
    20461 tctatctcag gcttgaaaaa tcgatgtctg agaatagcat agaagcatat ctgggtgata
    20521 tccgcaagct tgaggtattt ttcaccggtg aaggcggtgc cattgaacct gccgatgtta
    20581 cgcataacgg actgaagaag tttctaaatc atatctctga gggaggaatc agtccgcgtt
    20641 cgcaggccag gttaatatcc ggtatcaggg cgttttacag gtttctgctg atggatggag
    20701 agatcagtga aaatccatcg tcactgctgg aatcacccag gaccggatta aggctgcccg
    20761 aagtcctgac agtaggcgaa atcgacagaa tggtagggat aatcgattta agcaagcctg
    20821 aaggacacag gaacaaggca ataattgaaa ctatttatgg ctgcgggctt agggtttctg
    20881 agctcgtcaa cctaaggctc accgacatcc accgcaatga gggttttgta aagataaccg
    20941 gtaagggtaa taaacagagg ctggtaccga ttggccacag gacactcagg gaaatagacc
    21001 tttacaaaat cgacaggaac cggctgcccg ttattcacga tgaaaatatc gtttttctca
    21061 acagaagggg ccggaagctt acaagatcaa tgatatttgc gattaccagg gatctggcag
    21121 aaagagcagg aataaaaaag aatatcagtc cgcatacact ccgccattca tttgcaacac
    21181 acatgataga ggccggtgca gatctcaggg cggtgcagga aatgctgggc cacgagtcga
    21241 tcatcaccac ggaaatatat acccacattg accggagctt tcttcgcgac acgcttataa
    21301 tgtttcatcc aagagcctga ttatttttat cttttctgaa atccaatttt aattttatct
    21361 ttgcaactta tttcgaaaat ccaatattta agttaatctc aaattcataa aagtatgtcg
    21421 aaaataaaaa gagtttattc ctttggcagg aagacagcgg aaggtaaagc tgacatgaaa
    21481 aaccttcttg ggggtaaagg tgccaatctt gccgagatga gtctgcttgg tctgccggtt
    21541 cctgccggtt ttacgatcac cacggaagca tgtaatgagt attatagcaa caactgtact
    21601 cttcccgaag gtctgatggc tgaagtgtgg gaagcaatga aagttacgga agagacaatg
    21661 gggatgaagt atggtgatcc cgagaatgct ttgcttgtat catgccgctc aggggcacgc
    21721 agttcaatgc cggggatgat ggacactgtg ctcaatattg ggctttgtac cgccacgatt
    21781 cctggtttcc tcaaaaagac aaataatcag cgttttgtat gggattccta ccgccgcctt
    21841 attatgatgt atgccgatgt tgttatggaa aaggcagaag ggattgaacc cggagagggc
    21901 aagggcatcc gtaagcagct cgacgaaatg cttgatgaat ttaaacataa gaaaggatat
    21961 caatctgata ctgacattac tgccgatgaa ctcaaagaac tttccgagaa attcagggaa
    22021 agggttaaac aagtgctcgg caagcccttc cctgatgatg caagggaaca gctggaaggt
    22081 agtattaagg ctgttttcaa aagctggaac gggaagaaag cagtatcata ccgccgtatt
    22141 gaaggaattc ccgacgactg gggtaccgca gtgaatgtcc aggccatggt gtttggcaac
    22201 atgggcgacc actctgccac aggggttgca tttacccgca atccggcaac cggtgagaat
    22261 ctgttctacg gcgaatggct ggtgaatgcc cagggggaag acgttgttgc aggcatcagg
    22321 acgccgagtc cgcttaacaa tgagaccaag aacgagcaga acaggcacct tcaaagcatg
    22381 gaagaagcca tgcccggaac atacagggaa ctttgccaga taagggagat tcttgaaaaa
    22441 tcattccgtg acatgctcga catcgagttc accatccagg aaggcacact ttatatgctg
    22501 caatgccgcg ccggtaaaag aaccggtact gctgccctga atatggctat ggatatgctc
    22561 agggaaaacc ttattgacga aaaaacggca gtgttgcgcg ttgatcccgt ccagcttgat
    22621 gagcttcttc atcccatctg tgatccggag gctgagaaaa aagtcagacc tgtggtcaag
    22681 ggccttccgg ccggacctgg tgctgctgtc gggaaaatag tattctctgc cgaggatgca
    22741 gttgtctggg cccgtaaagg gaacaaggtc atactcgtcc gcgaagaaac gaatccggaa
    22801 gacgttgagg ggatgagagc cgcagaaggc atactgaccg cacgcggagg catgacatcg
    22861 catgccgcac ttgttgcacg cggatgggga aagtgctgca tcgtgggggc aggcagcctg
    22921 cacgttgacg cagcagcaaa gaaggcaaag gtcaacggaa ccgatattgt ccttaaggag
    22981 ggtgatgtca ttactctcaa cggtaccaaa gggaacgtgt accttggcga actggatctg
    23041 atggatgcga ctgaaaatcc gcagttcaaa agtttcatga ccatggttga caaataccgg
    23101 aaaatgggag tccgcaccaa tgctgaaacc gttgaagatg caaaaattgc ccgcgatttc
    23161 ggagctgaag ggatcggact tttccggaca gagcatatgt tctatggcaa gaattccgag
    23221 cagccgttgt tccttctccg caaaatgatc ctgagtgcca atgaagcaga aaggcgcagt
    23281 gcccttaatg aactcttcat ttacataaag gaagacatga aggaaactct tgaagcaatg
    23341 gatggcctgc ctgtaacttt ccggctgctt gatccgcctc ttcatgagtt tgttccccag
    23401 ggtgcagaaa aacaggctga actggcaaaa gcacttggaa ttaaggaatc agaagtggtt
    23461 aaaagaagtg aagcccttca tgaatcaaat cccatgatgg gacaccgggg tgtcagactg
    23521 ggtattacct atccggaaat aacggaaaca caggtccgtg caatgtttga agcctcggca
    23581 gagctgatca gggcaaaaaa acatcccaag ccggagctca tggttcccgt cacctgcgat
    23641 gtgtcggaac ttgatgtcac caaaaagata gtggacgccg tatatgctga ggtcctgaaa
    23701 aaaacgggtc ttgaaaagat agattacaag tatgggacga tgatcgaaat acccagggct
    23761 acccttctgt ctgaccgtat ggcaaaatcc gccgaatttt tctcattcgg aaccaacgac
    23821 cttacccaga tgacatacgg attcagccgc gatgatatcg gaggattcct ccacgactat
    23881 ctcgacaagg gaatgctcag cgccgacccg ttccagacca ttgacctgga tggagtcgga
    23941 cagctgatta aaatgtcggt cgaaaaggga cgtgccacca ggcccgacct gaaagtcgga
    24001 atttgcggag aacagggagg cgaccctgca tccgttgaat tctgctttaa aaccggactt
    24061 acatatgtaa gctgctcgcc tttcagggtt cccattgcaa ggctcgcagc agcacaggca
    24121 tcgataaggt acgggaaata aataaccgga actttactga ctgaaacaga ggctgtctca
    24181 aagatcattt ttaaggtgaa tgcctgttaa gcagcaaagg ggaacaaaag gaataattat
    24241 attcattcga tcccggggtg cggcttcaaa cgggtttacg ggaaaatggt acaaaggcat
    24301 taaaccacct ttctttgaga catcctcttt tgttttttct gacaggcctg acggcggtaa
    24361 tctaaataaa ataattatca tcccgtattt taatgtttta acactatctt tgcaattaat
    24421 tagaaaaaat ataccgcata aaggtttaaa ttttatttat ttctatcttt gggatgaact
    24481 gactttcact aacaaattat acaatgatca ctgaaaaaga attggcagaa tacggcatta
    24541 tgagccctct tgagattatt tataatccgt catacgataa gctctacgag gaagagacac
    24601 ggccggatct gaaaggttac gaaaagggaa cggtaaccac taccggtgcc gtggcggtcg
    24661 ataccggtgt ttttacaggc cggtcgccaa gtgacaagta tgttgttctg gatgatacaa
    24721 ccaaaaatac cgtatggtgg aaatcagaaa taaacaagat ctccgacaat aagcccatca
    24781 cacaggaaat atgggaacac tgccgtaacc tggctgccgg acaattgtca ggaaaaagag
    24841 tattcgtggt tgactgcttt tgcgggacca acaaggacac ccggttgagt gtgaggtttg
    24901 ttatggagat tgcctggcag gcacactttg taaagaacat gtttatacgt cctgataccg
    24961 gggagctgaa taactttgtt cccgagtttg ttgtacttaa tgcatccagg gtaatcaatc
    25021 ctgactggaa gaagcaggga ttaaactctg aaaacttcgt ttttttcaat ctgacttcca
    25081 aaatggccgt tattggagga acctggtacg gaggtgaaat gaaaaaaggg attttttcgg
    25141 taatgaacta ttatcttcct ctcaaaggca ttgcttcaat gcattgttcc gcaaatgtcg
    25201 ggaagaaagg tgatgtggca atctttttcg ggctctccgg caccgggaaa accaccctgt
    25261 cggccgatcc caacagggca ctgataggcg atgatgaaca tggctgggat gaggagggta
    25321 tattcaattt tgaagggggc tgctatgcaa agtgtattaa tttaagcaaa aaaaatgagc
    25381 ccgatattta caatgccatt aaacgtgacg ccctgcttga aaatcttgtg atcctccccg
    25441 atgggacaat tgacttcaac gacgattcca aaacggaaaa taccagggta tcgtacccga
    25501 tttatcatat acagaacatt gtaaagccgg tatccagggc tggtcatgca agcaaggtaa
    25561 tctttcttac cgctgacact ttcggcgtat tgccggcagt gagccgcctt accgaggagc
    25621 agactgaata ttacttccta agcggataca ctgcaaaggt ggcaggaacg gaaaggggta
    25681 tcctggaacc cgtaccatcc ttctcggcat gttttggagc agcatttttg atgctcgatc
    25741 cgataatata tgcaaaggaa cttacgaaga agatgaagga acacggagcc gaggcctggc
    25801 tggtaaacac aggatggatc agcggttcat acaatacggc ttcgagaatt gatatacccg
    25861 tcaccaggaa aataatcaat tcaatacttg ataactcaat cagcaaatat ccgtttacaa
    25921 gtcttcctgt gttcaacctt aacataccat cccgtatcga tgatatcgat ccgaacctcc
    25981 tcgatcccca gaaagcctgg gattcagcgg ttaaatggag gattgctgca accgacctgg
    26041 ccctgaaatt cataaacaat ttcaataagt tctcaggaaa tgacgaaaca atgagcctgg
    26101 tcgctcatgg cccgagggtc atttgaaatc tgccggaatg catgtcctga aatataccgg
    26161 agttttcatt ctttcctatc ttatcggttc agcagcaaca gctgtatggg caggccggat
    26221 attccacaaa acggatgtaa ggaagcatgg gagcggcaat gccggagcaa caaacgttat
    26281 aagggtttta ggttggaaaa ccgggattcc cgtacttttg attgatattt tcaagggata
    26341 cctggctgca tccctgccgg ttttatttaa acttgccgga cccgggactg cccagttgat
    26401 caacctccag atattgacgg gtctgcttgc ggtttgcggc catatttttc ccgtttatgc
    26461 cggattcaga ggcggtaaag gagtcgcagc aatcttcggg gtcctgctgg ccattcatcc
    26521 gctgctcacc ctttcatgca tcggagtatt tctcctggta tttcttgcaa ccggatatgt
    26581 ttcacttgga tcattgagcg ccggcgttgc ctttcccgtt ttcctcttcc tctttttcga
    26641 taccccttcg gtgattttca aagttttttc agttgtaatt gcaattatgc tgatcatcac
    26701 tcacaggaaa aacatttcaa ggcttttaaa aggcgaagaa tcaaagcttc taagaaaaaa
    26761 taccggtacc ggaaagaact agatcctgtc agtactgctc aagcagcgct attcttttct
    26821 tgatgggagg atgggtggca aacaggccgg aaaatgaaaa ggtgctttct ttctctttca
    26881 ggccgggatt ttctataaac atctgtgcaa catcctttct ctttactgct tcgatcacag
    26941 gatcttccga tatcttcctc aatgccgagg ccagcgaaag gggcctcctg gtcagttcag
    27001 ccgatcctgc atcggcaagg tattcccttt tccttgaaag agcaaatctg aacagcattg
    27061 caagcaggta gccaatcagg gccaggacca gtgctatcag tattccggcc ccctctttcc
    27121 tgcctcttcc tgatcttccg aaacgaaggg aacggaacag catttcagca atgaatccga
    27181 atattcccac aaaaacaatt gaaataataa gcagccttac atcccggttc cggatatggg
    27241 taagttcatg cgcaataact gcctcaagtt catcatcctc cagcttattg atgatcccgc
    27301 gcgaaagcga caccgtatat gttgacggat tgaccccgct tgcaaaggca ttgagggaat
    27361 catcttcgat aatattcact ttaggcattt tcatccctga tgaaatacaa agattctcca
    27421 ccaggttgta gactcttttg ttttgctttc tctcgagcgg ccgggaattc gtagctgaat
    27481 tgataatcgc tgtgtgcgaa aaccatgcaa tgacaaacca gattgcaact cctgctgtaa
    27541 tccaggggac cgttccaaga aatgagtggt ttatgctgaa cagactgacc tgctcatttc
    27601 cactcaccat cgacataaaa aagaaaaaca gccaggtcag ggcataaaaa acaagcggga
    27661 acatgatcag aagcaatacg gagttcacgt tatttttcct gatctggctc tgaagtccaa
    27721 aatacttcat ttactggaac ttaatttgcg gaggttcctc aatcacagta cgtttatcac
    27781 ccaggtcaaa cataggttca cgtttgaaat tgaacatcgt ggcaacaata tttgacggga
    27841 agacctcggt ggccacgtta agctcttttg tagccgagtt aaagaatctg cgtgcagctg
    27901 ctatcttgtt ttcaacatcg gaaatctcat tctgcaaatc catgaaattc tggctggctt
    27961 taaggtcggg atatgcttca accgaaaccc ttaaaccatc gagtgcactc gaaagacggg
    28021 cttcggcagc tattttatcg tttatcgttg acgctttcat ggcatttgcc ctggcctcag
    28081 taatctccgt aaggacaccc ctttcatgaa ccatatatcc tttaacggcc tcaaccagct
    28141 gcggaatgag atcatgccgc tggcgcagct gaacatcaac atcggcaaat gcctgttccc
    28201 tgctattcct cagtcttaca agcctgttat aaagagagat cgccatcagg atgaaagcga
    28261 cgatgattcc taaaataacc agtaatactc ccattgttgt ttttattaga ttaataatta
    28321 tatttaattc agataaaatt ataaataaac ccggatatta ttacaaaagg aaaatcaata
    28381 acctgaaaaa gggtaaatta ataaccagtt caacctgaat attatggaaa agagtttaaa
    28441 aggaacaaag actgaacaga atttgctgaa agcatttgca ggtgaatcgc aggcaaaaaa
    28501 cagatatgaa tttgcagcca aggttgcgaa ggaagaagga tacgagcaga tagccggtat
    28561 ctttcttgaa acggcgcttc aggagcagca gcatgccaaa agatttttca acttcctcga
    28621 aggcggaatg gttgagataa cagcctgcta tccggccggt aaaacaggaa caacaaagga
    28681 taatctgaat gcagcggcag aaggcgagca tgaggaatgg agcgagctgt atcccgcatt
    28741 tgcagaggtt gcggaagaag agggttttaa gaatattgcc acagccttca gggcaattgc
    28801 agcggcggaa aaaggacatg aggagcgata caggaaactg ctggaaaacc tcatgaaaaa
    28861 caaggttttt gaacgtgatg ataaagtatt ctggtattgc aggaaatgcg gttacattca
    28921 tttcggcaca aaacccctga agaactgccc ggcatgcctg cacccgatgg gctactttga
    28981 agtgaaagcc gacaactact agcagatgca tgacggaaaa gagatgctcg ggctgatact
    29041 caggcgccag agtgaaagga aatacagcaa caagccggtt gaacccgaca ggcttgaaag
    29101 gatcatagaa gcaggaagaa tggcaccttc agcctgcaat gcccagccat ggaaatttgt
    29161 ggtggccacg gatgctgaag tgattgccgg actggcaaaa gccgcatctg caaagcttct
    29221 gggcatgaac ggctttgtcg gtcaggccaa tgtcattgtt gtcattgtca gggaaaaacc
    29281 caatattact tcacgcgtgg gcggggtggt caaaagcaag gattattcaa gcttcgatgt
    29341 agggattgcc gtcaacagca tgatccttca ggctgctgta gaaggcattg gttcatgcat
    29401 cattggctgg ttcgatgaaa agg
//
LOCUS       DAOOYG010000022        28042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1473_length_28042_cov_7.643049, whole genome shotgun sequence.
ACCESSION   DAOOYG010000022 DAOOYG010000000
VERSION     DAOOYG010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28042
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1473_length_28042_cov_7.643049"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PK288_03560"
     CDS             complement(<1..758)
                     /locus_tag="PK288_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPM18028.1"
                     /translation="MRKSLIQTIKFLIFLVIGIILLWLAFRNVETRKLIAGLQDADYK
                     WVILSVIFGLFAYLSRTRRWVLLIKPLGYNPSFKNSFFSLMTGYLANLALPRLGEITR
                     CVALGKKEKIPVDQLFGTVVIERTIDLISLLSITVALVLQRGEQINQFLKVSILIPLQ
                     QKVFSMFGFTWLVWLVLLIVMILALFLLVKFRHRLRKIRFFAKIIDTVKGFVNGLKTI
                     IHLKNKWEFIFHTVFIWFNYTLMTWIVVYAIDSTS"
     gene            complement(760..1110)
                     /locus_tag="PK288_03565"
     CDS             complement(760..1110)
                     /locus_tag="PK288_03565"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPM18029.1"
                     /translation="MVIEVLKSKIHKVTVTEANLHYVGSVSIDEDLMDASGIIENEKV
                     QVLNVNNGERLETYAIRGERGSGQICLNGPAARKVVVGDVVIIMSYAFMEPGEAKSFR
                     PAIIFPDTKTNKLI"
     gene            complement(1161..2015)
                     /gene="panC"
                     /locus_tag="PK288_03570"
     CDS             complement(1161..2015)
                     /gene="panC"
                     /locus_tag="PK288_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPM18030.1"
                     /translation="MKVISKAEELEKAIGGTLLSPVGFVPTMGALHAGHLSLVNRATD
                     ETPLVVTSIYVNPTQFNDPKDLENYPRTLEEDLALLKNHLRTDDIVFTPSDREIYPEK
                     DTRQFHFGNLDNVMEALHRPGHFNGVAQVVSRLFAIVRPDIAYFGQKDFQQLAVIREL
                     VRQTGNRVRIVGCPIIREADGLAMSSRNRLLDPEIRKNAATIFKALNLASERFKSKEI
                     AELKKDVKDMIEKIPGFTVEYFEIADDIELIPLKNKSEVREGCRYFGCIAVMAGKIRL
                     IDNIEIQV"
     gene            2185..2991
                     /locus_tag="PK288_03575"
     CDS             2185..2991
                     /locus_tag="PK288_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPM18031.1"
                     /translation="MESKKVLFISQEITPYLAETKLSKIGRYLPQHIQEKGKEIRTFM
                     PKFGSINERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQSARMQVYFIDNDDYFQR
                     KYTVADSTGKEFEDNDERALFFARGVIETVKKLRWAPDIVHCHGWMTSLVPVYLRSIY
                     SDDPLFHDYKIVYSVYNNGFKTPFRPTFADKLRYDGVDGENLKLVKNPDFINVSKLAI
                     KYSDAIIIGSEEINSELYRHIKSVNKPYLDYHDESTYVDAYNDFYDKILS"
     gene            3241..5241
                     /locus_tag="PK288_03580"
     CDS             3241..5241
                     /locus_tag="PK288_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPM18032.1"
                     /translation="MNVSDDYRSLSVPEAEQLKANGCKSSDWAKIQVREGFDPSRCVN
                     VVFSGYNRLGSFRGSCTDESGIEYKCGIYNATIHNCTIGSDSHINNIGDCLANYRIDD
                     RVIIRNCGKLNTEGVTSFGNGTEVAVLNETGGRSVKIWDRLSAHEAYIIALYRHRIKA
                     VRKIEEMVSEYSRSVSSDTGYIGYGSRILNCQTITNVKIGPHSRIEGVIRLHDGSVNS
                     CSEDPVFIGPGVIMEHFIVCSGSIVTESTLIDKCFIGQGCVLGKHYSAENSLFFANCG
                     GYHGEACSIFAGPYTVTHHKSTLLIAGIFSFMNAGSGSNQSNHMYKLGPIHHGIMERG
                     SKTTSDSYLLWPARIGPYTLVMGRHYRNMDTSSLPFSYLIEKNDESILVPGINLRSVG
                     TIRDAQKWPLRDRRKDPVKLDQVNFNLLSPYTIRKMIEGKNLLTRLRTVSGSVSASYT
                     YNNMMIKEASLERGIELYQTGIDKFLGNSIISRLEDGEITSNEELRRRLTPDQETGRG
                     EWVDLAGLIAPKQVVSELLDDIESDKINSIESVVEVFREMHRSYYDWEWTWACERIEE
                     EVGKPVASFTSEDVIRLVERWKKSVVSLDRLLYEDARKEFTISSMTGFGIDGDEEVRK
                     LDFEQVRGDFDSNTVVAAIQEHIKQKSSLGDKLIERMRRISS"
     gene            complement(5248..6309)
                     /gene="rfbB"
                     /locus_tag="PK288_03585"
     CDS             complement(5248..6309)
                     /gene="rfbB"
                     /locus_tag="PK288_03585"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPM18033.1"
                     /translation="MKMGKSILITGGAGFIGSHVVRRFVTKYPHYRIVNADKLTYAGN
                     LDNLSDIEKCSNYIFEKADIVDKKTIAALFEKYGFDGVIHLAAESHVDRSISSPDEFV
                     HTNIVGTVNLLNAAMATWKNDHSGKLFYHISTDEVYGSLGKEGMFTEETPYDPRSPYS
                     ASKASSDHLVRAYWHTFGIPVVVSNCSNNYGPNQFPEKLIPLAINNIRNNKPVPVYGK
                     GENIRDWLYVEDHASAIDLIFHRGTPGETYNIGGNNEWRNIDLIKLLCRIMDQKLGRP
                     EGTSEKLITYVKDRPGHDLRYAIDSSKLQREFGWSPIPDFSEGLEKTVDWYLSNTLWL
                     ERVLSGDYEKYYDRMYSGR"
     gene            complement(6306..7334)
                     /gene="galE"
                     /locus_tag="PK288_03590"
     CDS             complement(6306..7334)
                     /gene="galE"
                     /locus_tag="PK288_03590"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPM18034.1"
                     /translation="MKEKILVTGGTGYIGSHTVVELINEGFGVVIVDNLYNSEAEVVD
                     RIAQITGVKPQLEVFDLCDREKLNSFFGKYENLSAIIHFAAYKAVGESVKKPLDYYRN
                     NLLSLINLLDLMKQYQIPHFVFSSSCTVYGQPEKLPVTEDAPMQPAVSPYGNTKQICE
                     EIIRDTVVSGVGIKAIALRYFNPIGAHPSALIGELPRGIPENLVPYITQTAYGLRDEL
                     KVFGDDYDTPDGSCIRDYLHVVDLAKAHVTAVRRLIEEKNKSGYEVFNLGTGKGVSVF
                     EAINSFERATGVKVKYRVTGRRPGDIVKIWADPSYANKELGWKTMSSLDESMETAWKW
                     EKYIRNKK"
     gene            complement(7797..8759)
                     /locus_tag="PK288_03595"
     CDS             complement(7797..8759)
                     /locus_tag="PK288_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18035.1"
                     /translation="MDSSSKNFGLIGVAGFVAVRHLKAIKDTGNNLLASLDKFDCVGL
                     LDGYFPQSDFFVEFERFDRHLDKLKRAGTKIDYVSICSPNYLHDSHIRFALRHQAVAI
                     CEKPLVLNPWNIDALQEIEKETGRKIYTVLQLRLHPKIAELREKIRNGPKDKIYDIDL
                     SYITSRGNWYHISWKGDIQKSGGVATNIGIHFFDMLGWIFGDTTKIIVHVSEPNKAAG
                     YIELEKARIRWFLSIDYNDLPAEAKKSGKRTYRSITVEGEEIEFSEGFGDLHTATYRE
                     ILAGRGFGLNESRQSIITAFTIRNSKPVGLVGEYHPLLQAHNNL"
     gene            complement(8763..9368)
                     /locus_tag="PK288_03600"
     CDS             complement(8763..9368)
                     /locus_tag="PK288_03600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPM18036.1"
                     /translation="MEKEYFAHETAVIDEGCKIGRGTKIWHFSHVMSGSEIGDNCNIG
                     QNVVISPGVKLGRNVKVQNNISVYTGVICEDDVFLGPSAVFTNIVNPRSAVARKDQYV
                     KTIVEKGATIGANATIVCGNRIGRFAFIGAGAVVTRDVAPYALVMGNPARQKGWMSEY
                     GHRLDFDNTGTASCPESGEKYILKDGMVTKIIEQAPSAEIK"
     gene            complement(9371..10504)
                     /locus_tag="PK288_03605"
     CDS             complement(9371..10504)
                     /locus_tag="PK288_03605"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM18037.1"
                     /translation="MREIQMVDLRAQYENLKDEIDRAIQAVLQSTAFIKGPEVGMFED
                     ELKEYSGARHVVSCANGTDALQIAMMSLGLNHGDEVITTNFTFVATIEVLALLGLKPV
                     IVDPDPGTFNISPDSIRKAITPRTRAIIPVHLFGQCADMESILSIAAGHNLFVVEDAA
                     QATGARFIFSNGNVKKAGTIGTIGTTSFFPSKNLGCYGDGGALFTDDDELAGKIRSIA
                     NHGMKIRYHYNYVGINSRLDTLQAAILRVKLRHLDSYNEARRHAADYYDKAFKECQDI
                     MVPLRSSFSSHIFHQYTLKVKGGKRDELKRYLGERGIPSMIYYPGPLHMQNAYRYLGY
                     GENDFPVTNSLCREVLSLPVHTEMDREQLEYITGCVLEFFNNN"
     gene            10699..11688
                     /locus_tag="PK288_03610"
     CDS             10699..11688
                     /locus_tag="PK288_03610"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18038.1"
                     /translation="MDIKAFIRELLFSYDCVIVPGFGGFIGNYSPARIDRTMGTFSPP
                     VRQITFNRNLDHNDGLLVGKISMAAGLNYGDSRRNVEDFVRNVRSRLAKGETVVFDHL
                     GTFSLNHENSIQFEPEANLNYYLGSYGLESFHMGHYKGYDVRKRVSRHIDRDPVRRTS
                     VVKNLVRAAIMIPIVALLVIVPLKDQYSRKSGVESVTLNPLVTAEFENNRNAIDNDHS
                     ANTPAVEEISANITPDAPVPETGPANDEIPEVETGQNVYYIITGSFKSEENALSHIDL
                     LREEGFDPEIIRASNGFFRVNAMKCSNIGIAISKKDSLSRKFPGVWITKKRPT"
     gene            complement(11681..13714)
                     /gene="metG"
                     /locus_tag="PK288_03615"
     CDS             complement(11681..13714)
                     /gene="metG"
                     /locus_tag="PK288_03615"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPM18039.1"
                     /translation="MKSFKRHLITSALPYANGPLHIGHLAGVYVPSDIYTRYLRLKGQ
                     DVLSICASDEHGVPITLKARTEGITPKEVVDRYHELNKKAFEDFGIAFDIYSRTSNKV
                     HYETASDFFLTLYNKGEFTEKVTEQYYDEEAGCFLADRYIVGTCPQCGNENAYGDQCE
                     KCGTSLSPTELINPHSTISGSKPVLRETLHWYLPLDKYEGWLREWILEGHKEWKTNVY
                     GQCKSWLEQGLQPRAVSRDLDWGIPVPVDGAGGKVLYVWFDAPIGYISATKELTPEWE
                     KYWKDPDTKLIHFIGKDNIVFHCIIFPAMLKAEGSFILPENVPANEFLNLENDKISTS
                     RNWAVWLHEYLEEFPGKQDVLRYVLCTNMPETKDSDFMWKDFQAKNNNELVAILGNFV
                     NRSLVLTNNYFGGIAPARSKTDDYDEKVLSEIERIRNNIEPSLESFRFRDALREAMNL
                     ARLGNKYLADTEPWKIHKSDPEKVKTIINIALQITANLTIILEPFLPFTTKKLRGWLN
                     CGKFSWAEAGRSDLIPPGHAIKKPGLLFEKIEDDVIEKQINKLRASLKTNNDTGTTVI
                     PPKETVSFDLFSRIDIRTATVLEAEKVPRTTKLLKLTIDTGIDTRTIVSGIAEYFQPD
                     DLVGKQISIVANLEPRKIKGIESKGMILMAEDSGGKLVLVSPAEKTGNGSIIK"
     gene            13920..14828
                     /locus_tag="PK288_03620"
     CDS             13920..14828
                     /locus_tag="PK288_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPM18040.1"
                     /translation="MNKIEPLLLKKGDKVGLISPAWAIDEDKAGKAVAFLEEWGLKVV
                     PGRNLLKRWGPFAGTDEERASDLQIMIDDPAVKAVFCSRGGYGTLRIIGKTDFAPLKR
                     SPKWFVGFSDITVIHMWLNEVCGIVSVHGEMPLNYYESGKTDETFVSLHNVLFGGNTT
                     ISWTGRTYRFREMTGEVTGGNLSLIYSLIGTRAEPRTRGRILFIEDTGEYLYHLDRMI
                     SSLKLAGKLGRIEGLIVGGLDRMEDTKISWGITPEEVIKDAVREFSFPVVFDFPAGHI
                     PDNRAFYIGRNARITSDGKRVSMTFI"
     gene            complement(14925..16148)
                     /locus_tag="PK288_03625"
     CDS             complement(14925..16148)
                     /locus_tag="PK288_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPM18041.1"
                     /translation="MMNIKNKSGRISFGNASLMPKEEQLETAVRHRRITIGIPSDRKN
                     DEKRVALTPEAVNILVENDNEVIVQKGAGTGANYSDKDYSENGAVITDSPERVFSADA
                     VIKVAPFTLQEVDYLKGDQLVMSFLNVLALSEEILEKLIRKKVTAVALEKIKDSNDVM
                     PVVESMSEINGVTSILVASDYLSNHLGGKGVLLGGITGVTPTEVVIIGANTAGEYAAR
                     AALGLGSQVRIFDKSLFNLKKFQNKIGQRLQTSVLHPQVLKKVLKSADVLIGAIELED
                     LRPRYYITEDMVKTMKKGAVVIDLSIDRGGCIETTECRPLRDPVYEKHGVIHFSAWNL
                     PSRMARTASIALSNIFNPLFQNFAESGGITQLLKNDPGIRNGVYLFNGILTNETLGQK
                     FGMISKDLNLLISAF"
     gene            complement(16210..16623)
                     /gene="tsaE"
                     /locus_tag="PK288_03630"
     CDS             complement(16210..16623)
                     /gene="tsaE"
                     /locus_tag="PK288_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPM18042.1"
                     /translation="MRIIIESRRQLQSAAKKLLEYTKGKRILAFYGPMGAGKTTIIKA
                     VCRSLNATDQITSPTFTLINEYKTTSGNPLYHMDFYRIKKTGEAYDFGIEEYFYSGAY
                     CFIEWPEQIEEILPADTARIKITVGENEQRILELT"
     gene            complement(16642..18198)
                     /locus_tag="PK288_03635"
     CDS             complement(16642..18198)
                     /locus_tag="PK288_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HPM18043.1"
                     /translation="MRKIRILWTDDEIEALKPHIFFLKDKGYEIDTCSNGTDTIDLVK
                     QNPYDLIFLDENMPGISGIETLRLIKEIRTDIPVVMITKHEEEGIMEAAIGSEIADYL
                     IKPVKPNQILLAIKRITEQKRLVTEKTTINYRQEFSRIGNMISSAQTHTDWAELYRKI
                     TFWESELEKSTDPGMSEILRMQENDANNAFIKYITSNYLSWLDPEASNKPVLSPMLFT
                     KKIFPNVEQGRPLFFILIDNLRFDQWKTISAELSGLFRIIDEDIYFSILPSATQFSRN
                     AIFAGLMPSAIAAILPDFWVDDDQDEGKNNYEEELFKYQLTRKGFRYKWSYNKISSNL
                     EGRRVNDKLRQLMDNDINILVYNFVDMLSHARTEIGFIKELANDERAYRSLTRSWFVH
                     SPLFELLVTLSSQNVRIIFSTDHGTIRVQNPIKVIGDRKTSFNLRYKMGRNLDYNPRE
                     VFEVRFPEKAGLPKTNITSKYIFALNKDYLVYQNNFNYYAGYYKDTFQHGGISMQEIM
                     LPVACLEPVS"
     gene            complement(18195..20810)
                     /locus_tag="PK288_03640"
     CDS             complement(18195..20810)
                     /locus_tag="PK288_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM18044.1"
                     /translation="MILTGCSVEKNTATTRFYQGLTSRYNIYFNGYESFKAGLAKINR
                     GYKDDFSELLKVFEFSDPATVQLCSSDMDRAIQKASKLISLKSITARPELGNKRNLSE
                     SEKSLLEKKEFNEWVDDSYFLIAKARFYKQEYNEAASVFNYCIAEANDPGIKTESAIW
                     LARIGNETGDYNNSLRLLREIEITPSSPKSLKSLYYSTLADLYVKQKNYSEAVSPLSE
                     SIKNSSGKTQKYRLTYLLAQLYERLGDGKKATSYYRAVVKMNPPYDVEFNARINIAGV
                     FDVESGNQQEIGKELEKMLKDAKNTDFHDQIYYALGNLKMKQGNEAEAIKAYRKSAAS
                     NTGNNNQKGKSYLALADYFFGKSDFIPAGKYYDSAVFFLDQKYPDYRELMTKSRDLDA
                     LVAELTVIRTEDSLQNVAALPQAQRDALIAGIIEKINLAEREGRSSEYSDRYNMGQFY
                     ENQRRFEDNINQEGRWYFYNQAALTFGRTEFRRRWGDRRLEDNWRRMNKARVNVQQMA
                     SGQEDNDLKKTDTTMAASDYHKPEFYLKDLPLNDSLLSISNQKIANAYFNSGRIFMER
                     IADRPHSVQSFETLISRFPDHELVPETLYELYRINKDENSQKSETYRQRLLEKYPGSE
                     FARILSDPDYYNNKLAGIKEAERLYGNAFDAYTLENYALAVSTCDSILKKYPEDDLAP
                     KAMLLRAYSTARISDEKTFRTNLSIIVKTWPGSPESKKAEELMTHLNQERPELKLEED
                     KEIATELYVRDTISILTFALVISNPAFNLNQATFDVISHNIDNFTNENYRTEGTLVGN
                     AYLMITVSGFRNNNEARMYFNAFDAASIIRNIASSRFMTFLINSTNLKILGEDKDPGR
                     YLIFFRDNYSGGKEQ"
     gene            complement(20937..21176)
                     /locus_tag="PK288_03645"
     CDS             complement(20937..21176)
                     /locus_tag="PK288_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPM18045.1"
                     /translation="MENSNATRERIVRALNRVRPYLQNDGGDIELVEITDDMVVKVRL
                     KGACHGCPFSLQTLKAGVEQAIMQEVPEVKKVISA"
     gene            complement(21270..22370)
                     /locus_tag="PK288_03650"
     CDS             complement(21270..22370)
                     /locus_tag="PK288_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPM18046.1"
                     /translation="MFRKELILKALTKVIHPEKGKDIVALGIVAEIESGNEGISITLK
                     PEKSNDPFISSVKSSIVRAIKDDLGKDAVVSNIIIQPRTIVHETEKQSDSLLPGVRNI
                     VAVSSGKGGVGKTTIAVNLAISVARKGFSVGLLDADVFGPSVPKMINAENYKPEVFRR
                     NDTDHIVPLTKYGVKILSTGFFVDPSDALVWRGPMASNFLKQLMAQGEWGSLDFLFID
                     LPPGTSDIHLTMVQEVAVTGAIVITTPQEVALADAIKGITMFRSARINVPVLGLVENM
                     SWFTPAELPDNKYYIFGKDGGRKLAEKMNVPLLGQIPLVQSICEGSDSGDPVALGETI
                     SAQAFMKLAEQVVEKVEKRNRDQKPTVKVSVT"
     gene            complement(22445..22798)
                     /locus_tag="PK288_03655"
     CDS             complement(22445..22798)
                     /locus_tag="PK288_03655"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPM18047.1"
                     /translation="MSNNKQGNNDFFSGVYEVTKLIPPGRVTTYGAIARFLATGRSAR
                     MVGWALNASGGRSEFIPAHRVVNRKGMLTGKHHFGNSSTMRQLLENEGMIIENDIILN
                     FNEKFWDPSSELTDF"
     gene            complement(22788..23579)
                     /gene="trmB"
                     /locus_tag="PK288_03660"
     CDS             complement(22788..23579)
                     /gene="trmB"
                     /locus_tag="PK288_03660"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPM18048.1"
                     /translation="MKRSVNLIDDVGKNKLARWAELETFGNVIQAGAGHESGEDHPLK
                     GKWRDKIFGNNNPVVLELACGKGEYTLGLATIFPEKNYIGIDIKGARMWRGAKTASEK
                     GLKNTAFLRTRIEFINSFFTKDEVDEIWIVFPDPHNRKRNSNKRLTSPWFLNKYRLLM
                     KDGGLIHLKTDNQELFRYTEKLVKNNGLEILRSTDDMYVGNPDVSVPFFSFLPGIGVY
                     GTDEMIPERIFSLRTHYEKMFLDKGLKICYLAFKLNKNTIVKDEQ"
     gene            complement(23554..24036)
                     /locus_tag="PK288_03665"
     CDS             complement(23554..24036)
                     /locus_tag="PK288_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPM18049.1"
                     /translation="MPGNKLFFAGAILLLIITSSCTGDIVFTDSSKMPSGTWDLENVS
                     EFDVPVNDTITANNIFFTIRTGTAYPYRNIYLFVTTTSPDEKRITDTLQYYLADEKGK
                     WYGKGFGDIKELLLPYKTNVLFPVKGTYHIRVSHGMRVRDLRSVYDFGIRIEKIRKPD
                     "
     gene            complement(24023..25171)
                     /gene="ricT"
                     /locus_tag="PK288_03670"
     CDS             complement(24023..25171)
                     /gene="ricT"
                     /locus_tag="PK288_03670"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPM18050.1"
                     /translation="MTESEELSGQHGEEIHLPDTKYKPGCNKLDSFNWLKDIPYNQAE
                     YDLVEVRFKNTRKGFYKNINNLRLEIGDIVAVEASPGHDIGRVSMVGPLIIEQLKKLK
                     ILPSTDDLKKVYRKAKPVDILKWEEAKQLEIPTMLRSRQISEELKMNMKIGDVEYQGD
                     KTKAIFYYIADERVDFRQLIKVLAEEFRVRIEMRQIGARQEAGRIGGIGACGRELCCS
                     THITNFMSVTTTHAKYQELTLNPQKLAGQCSKLKCCLNFEFDCYLDAQKHFPPRDIPL
                     ELANSKAYYQKMEVHKNIYWYSSDPHSPVDLTAVPISRVREIQMMNRNGKKPDKLLIP
                     DEFHNPDIHSKDLLMDNSITRFDPSETKNTHRRQSARKRKNKKPNARK"
     gene            complement(25243..26373)
                     /locus_tag="PK288_03675"
     CDS             complement(25243..26373)
                     /locus_tag="PK288_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPM18051.1"
                     /translation="MRFSDIPGQQEIKAGLVKTVDQGRVSHAQLFAGPEGCGSFAVSL
                     AYAQFISCENRLPSDSCGKCKSCLKYEKLIHPDLHFVFPVIKDKKTKEQVSDNYISEW
                     REFVGQSPFFSLNNWLDFIEVGNAQGLIFASEASEIIRKLMLKTFESDYKILILWLPE
                     KMHIATANKLLKMIEEPPEKTLFLLVSEEPDKIIPTIASRCQMVKVPSFRLSDIAGYL
                     QSNFGIGDKQANDIARVANGNIVRAVEMCKNQDTTVLNLDRFKNLMRFAWKRDIISII
                     SWSEEMASIGREPQKNFLSFALRLVRENFMLTLGQGKNNIVYLTDDETAFSSKFHPFI
                     NQKNVHGFSEELNLAHAHVEANGNAKIIFLDLALKLTRLIRQ"
     gene            26716..27978
                     /locus_tag="PK288_03680"
     CDS             26716..27978
                     /locus_tag="PK288_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPM18052.1"
                     /translation="MKMIQDFDFKGVKAIVRVDFNVPLDKKTLVVTDDTRIRGAIPTL
                     RKIIGDGGSVILMSHLGRPEGRMEKYSLKPVLPVLEKHLGQSVKFADDCLGESAVSMS
                     AALKPGEVLLLENVRFYPEEEGKPVLPETATDEEKKAAKAELKLKQKEMARKLAGYAD
                     VYVNDAFGTAHRAHGSTAVIADFFPKDKIMFGFLINSELAAMDKVLNNAQRPFTAIMG
                     GAKVSDKILLIENLLNRVDNLIIGGGMTYTFVKAKGGKIGKSLCEEDKLDLALKIIEK
                     AGEKNVNLYLPVDSVNGDKFDNEANIRVTAVDAVDDGWMGLDIGEKTIEEFSGVIGKS
                     KTILWNGPMGVFEMEKFSKGTTAIAKAIASATSKGAFSLIGGGDSVAAINRNGLADKV
                     SYVSTGGGAMLEYMEGKKLPGIVAIRGE"
BASE COUNT         7195 a         6171 c         6257 g         8419 t
ORIGIN      
        1 gacgttgagt cgattgcata gacgacaatc catgtcataa gcgtgtaatt gaaccatata
       61 aagacagtat ggaaaatgaa ttcccatttg tttttaaggt gtattatggt tttcaatccg
      121 ttaacaaagc cttttaccgt atcgataatt tttgcaaaga atctgatctt ccgtaatctg
      181 tgtctgaatt ttaccagtag aaaaagtgca aggatcatga caatcaataa aaccagccag
      241 accagccatg taaatccaaa cattgaaaag accttctgct gaagcggtat aaggatgctt
      301 accttgagga actggtttat ctgctcacct ctctgcagga caagggcaac cgttatggaa
      361 aggagtgaaa taaggtcgat ggtacgttcg attacaacag ttccgaaaag ctggtcaact
      421 ggtatctttt cttttttgcc aagggcaacg caccgtgtta tttctccgag acggggcaac
      481 gcaagatttg ccagataacc tgtcatgagg gaaaaaaaac tgtttttaaa tgatgggttg
      541 taaccaagcg gttttataag gagtacccac cttctggtcc tgcttaggta ggcgaacaaa
      601 ccgaatatga ccgaaaggat cacccatttg taatcggcat cctgcagacc ggcaataagc
      661 ttccgagttt caacattacg gaatgccagc cataatagga ttatcccgat aacaagaaat
      721 ataaggaact tgatggtttg aattaaggat tttctcaatt cagatcagtt tattggtttt
      781 agtgtcaggg aatatgattg ctggcctgaa agactttgcc tccccgggtt ccataaatgc
      841 atatgacatt attatcacca cgtcgcccac aactaccttt ctggccgctg ggccattgag
      901 acagatctga cctgaccccc tttcccccct gatggcatac gtttcgaggc gttccccgtt
      961 gttgacgttg agaacctgaa ccttttcatt ttcaataatc cccgaggcat ccatcagatc
     1021 ttcatcaatc gacacacttc cgacgtaatg cagatttgct tccgttactg ttactttatg
     1081 aatttttgat ttcagaacct ctataaccat attttcggtg attggattag tgcgtaaatt
     1141 taattatctt tttcttgtga ttatacctgg atttcaatat tatcaatgag cctgatcttt
     1201 ccggccatca cggctatgca accaaaataa cggcatcctt ccctgacctc tgatttgttt
     1261 tttagcggaa tcagttcaat gtcgtcggca atctcaaaat attccacggt aaatcccggg
     1321 attttttcta tcatgtcctt cacgtctttt tttaattcag cgatttcctt gcttttgaac
     1381 ctttccgaag ctaagttcag tgctttgaaa atagtcgctg cattttttct tatttccgga
     1441 tccagcaggc ggtttcggct gctcatggca agcccgtcag cttccctgat tatagggcag
     1501 cccactatcc tgaccctgtt tccggtctgt cggaccagtt cccttattac tgcaagctgc
     1561 tggaaatcct tctgcccgaa ataggcaatg tcgggcctga caatggcaaa gagcctgctt
     1621 acaacctgag caacaccatt gaaatgacca ggcctgtgca aggcctccat aacgttgtcg
     1681 aggttgccga agtgaaactg ccgggtatct ttttcggggt aaatctccct gtcggaagga
     1741 gtaaaaacaa tatcgtcagt tctgagatga tttttaagaa gagcaaggtc ctcttccaag
     1801 gtcctggggt aattttcaag atctttcgga tcattgaact gggtcggatt gacataaatg
     1861 cttgtaacta caagaggagt ttcatctgtt gccctgttta caagtgacag gtgccccgca
     1921 tgaagggctc ccattgtagg aacaaatcca acaggggaaa gcaatgtacc gcctattgct
     1981 ttctccaact cctctgcctt gcttattacc ttcataaact gtgaatatta tgcaaaccta
     2041 agttaaaatt gacaattatc tgcccttttt aacattatta aaaataatcc ggggctttgt
     2101 acccgacaaa gcatgttgat aattttgtca ttttttatta gtatctttgc aaattggata
     2161 cgataggtat atcgaggcag gtaaatggaa agcaagaagg tattattcat ttcgcaggag
     2221 atcactccat acttggctga aacaaaatta tcaaaaatcg gaaggtatct tccccagcac
     2281 attcaggaaa aaggtaagga gatacggact tttatgccga agttcggaag tattaatgag
     2341 cgcaggaacc agcttcatga ggtgatccgg ctttcgggaa tgaaccttat aattgatgat
     2401 accgatcacc cgcttataat taaagttgcc tcgatacaat ctgcaagaat gcaggtctat
     2461 tttattgaca atgacgatta tttccagcgc aaatatactg ttgccgattc aactggcaag
     2521 gagttcgagg ataatgatga aagggcactt ttttttgcca ggggcgttat agaaactgta
     2581 aaaaaacttc ggtgggcacc cgacatcgtg cattgccacg ggtggatgac atccctggtc
     2641 cccgtatacc tgagaagcat ttacagcgat gatcctttat ttcatgatta caagatagtt
     2701 tattcggttt ataataatgg ttttaaaact ccgttcagac cgacatttgc agataaactt
     2761 agatatgacg gcgtggacgg ggaaaacctg aaactggtga agaaccctga tttcattaac
     2821 gtctcgaaac ttgcaataaa atactcagat gcaataatta tcggaagcga agagatcaat
     2881 tccgaactgt accggcatat aaaatcagta aacaaaccct atctggatta tcacgatgaa
     2941 tccacctatg tcgacgcata caatgatttt tatgataaga tactctcctg attttatcat
     3001 ttaattcggg aatccggttt ccattcacaa tatttttcaa atttccttgt tcattattca
     3061 attagttctt agggaaataa gtcaggtata aacagtcctg ggtgatcatt gatccggccc
     3121 ggattcttat actccctgat aaattgaata tagttgtccg gaactttggc cacgcgaggc
     3181 tgatcagccg gggtaccgtg taaggccggg ggccgttgaa tgatattaaa acctttaaaa
     3241 atgaatgtgt ctgacgatta caggagcctc tcggtcccgg aggccgagca gctgaaggca
     3301 aacggatgca aaagcagcga ctgggcaaag atacaggtca gggaaggatt cgatccgtca
     3361 agatgtgtca atgttgtctt ttccgggtac aacaggctgg ggtccttcag gggatcctgt
     3421 acggatgaat cgggcattga gtacaaatgc ggtatttata atgcaactat ccataactgt
     3481 acaatagggt cagattccca tataaacaat atcggagatt gcctggcaaa ctacaggatt
     3541 gatgacaggg tcataatcag aaactgcgga aagctgaata ctgaaggcgt cacatccttt
     3601 ggcaacggaa ctgaagtagc agttttgaat gaaacggggg ggcgttcggt gaagatttgg
     3661 gacaggcttt ctgcccatga agcctatata atagcactct acaggcatag aataaaggct
     3721 gtcagaaaaa ttgaggagat ggtctcggaa tattcccggt cagtcagctc cgacacggga
     3781 tatatcggct atggttcaag aattcttaat tgccagacca taaccaacgt gaagatcgga
     3841 ccgcatagcc gcattgaagg tgttataagg ctgcatgacg gatctgtcaa cagttgcagt
     3901 gaggatccgg tttttatagg cccgggggtt atcatggaac attttatcgt gtgttcagga
     3961 tcgattgtta ccgaatcaac ccttatcgat aaatgtttca ttggtcaggg ctgcgtactg
     4021 gggaaacact attcggccga aaattcactt tttttcgcca actgcggggg ctatcatggt
     4081 gaagcatgct caatatttgc cggtccgtat actgttacac accataaatc aaccctgctg
     4141 atcgcgggaa ttttttcctt catgaatgcc gggagcggat ctaaccaaag caaccacatg
     4201 tacaagcttg gtcccattca ccatggtata atggaaaggg gttccaaaac aaccagtgat
     4261 tcctatctgc tctggccggc acgtatcggg ccttataccc ttgtgatggg caggcattac
     4321 aggaacatgg atacttcatc actgccattt tcatatctta ttgaaaaaaa cgatgaaagc
     4381 atccttgttc cggggatcaa tctgcgaagc gtggggacga taagggatgc acaaaaatgg
     4441 cctttgcgcg atagaagaaa agatccggta aagctcgatc aggtgaattt taatcttcta
     4501 agtccctaca ctatcaggaa aatgatcgaa ggaaaaaatc ttctgaccag gctcagaaca
     4561 gtctccggat cagtttctgc atcatatacc tataacaaca tgatgataaa ggaagcttcc
     4621 cttgaaaggg ggatagaatt gtatcaaacc ggtatagata aattcctggg aaattcgata
     4681 atcagcaggc ttgaggacgg tgaaatcaca tcgaatgagg agctccggag aagactgact
     4741 cccgatcagg agacaggcag gggtgaatgg gttgaccttg ccgggttaat agctccaaaa
     4801 caggtagtct cggaactgct ggatgatatt gaatcggaca aaataaactc cattgagtct
     4861 gttgtggaag ttttcaggga aatgcacaga tcctattatg actgggaatg gacctgggca
     4921 tgtgaacgga ttgaagagga agtaggcaaa cctgtggctt catttacctc agaggatgtg
     4981 atacgacttg ttgaacgatg gaagaaaagt gtcgtttccc tcgacaggct tttgtatgaa
     5041 gatgcaagga aggagtttac aatttcttcc atgactggtt tcgggataga cggtgacgag
     5101 gaagtcagaa aactcgattt cgaacaggtg cgtggcgatt ttgactcaaa caccgtggtt
     5161 gcagccatac aggagcatat taagcagaag agctcccttg gcgataagct gattgagcgt
     5221 atgaggagga tcagcagcta attggggcta tcttcccgaa tacatcctgt cgtagtattt
     5281 ttcataatca cccgacaata ccctttcaag ccacaaagtg tttgaaagat accagtcaac
     5341 cgtcttttca agtccttctg aaaaatcagg gatgggcgac cagccaaatt cacgctgaag
     5401 tttggatgaa tcgatggcat acctgagatc atggcccggt ctgtccttga cataggttat
     5461 cagcttttcc gaagttcctt caggccttcc cagcttttga tccatgatcc tgcatagcag
     5521 ttttatcaga tcaatgtttc tccattcatt gttgccgcca atattatatg tttccccggg
     5581 agtgccacga tgaaaaatca gatcgattgc tgaggcatga tcttcaacat aaagccagtc
     5641 gcggatattc tcgccctttc catagacggg aaccggtttg ttgtttctga tattgttgat
     5701 ggccagcgga atgagttttt caggaaactg gttgggtccg taattatttg aacagttgga
     5761 gactaccacg gggatgccaa aagtatgcca gtatgccctg acaagatggt cagaactggc
     5821 ttttgatgcg gaatagggac ttcttggatc atagggagtc tcttccgtga acattccctc
     5881 ttttccaaga gaaccgtata cttcgtcggt ggagatgtga tagaacaatt ttccgctatg
     5941 gtcatttttc catgtggcca tcgcggcatt gagaaggttg acagttccga cgatattggt
     6001 atgaacaaat tcgtcgggtg aggatattga tctgtcgaca tgcgattcag ccgcaaggtg
     6061 aatgacgccg tcaaatccgt atttttcaaa aagggccgcg atagtctttt tatcaactat
     6121 atcggccttt tcaaaaatgt agttcgaaca cttttcaata tctgaaagat tatcaagatt
     6181 tcccgcatat gtcagcttgt cggcattgac tatcctgtaa tgaggatatt ttgttacgaa
     6241 tcttctgacg acgtgcgagc ctataaaacc tgcaccaccg gtaataagta tgctttttcc
     6301 cattttcatt tcttgtttct tatatatttt tcccatttcc aggcagtctc cattgattca
     6361 tcaagggaac tcatggtttt ccatcccagt tccttgttgg catatgatgg gtcggcccat
     6421 attttcacga tatcacccgg ccttctgccg gtgactctgt atttcacttt gacacccgtg
     6481 gctctttcaa aggaatttat tgcttcgaaa accgatactc ccttacctgt tccaaggttg
     6541 aagacttcat agccggattt gtttttttct tcaatcagtc ttctgaccgc agtcacatgt
     6601 gctttggcca gatcaacaac atggaggtaa tcacgaatgc atgaaccatc gggcgtgtca
     6661 taatcatcgc cgaagacctt cagctcatcc cttaatccat aggcggtttg ggtaatgtag
     6721 ggtaccaggt tttcaggaat gccccggggc agctcaccta ttagggcgga aggatgtgcc
     6781 ccgatggggt taaagtatct taatgcaatg gctttgattc cgactcccga taccacagtg
     6841 tccctgatga tctcttcaca tatctgcttt gtgttaccat aaggggatac ggcaggctgc
     6901 attggagcat cctctgtaac aggaagcttt tccggctgtc cgtaaacagt gcatgacgat
     6961 gaaaagacaa aatgggggat ctggtattgt ttcatcaggt caaggagatt gatgagtgaa
     7021 agcaggttgt tcctgtaata atccaggggt tttttcactg attcaccaac agccttgtat
     7081 gcagcaaaat ggataattgc cgagagattt tcgtatttcc cgaaaaagga attcagcttt
     7141 tccctgtcgc acagatcaaa aacctctagt tggggcttta caccggttat ctgtgcaatt
     7201 ctgtcaacga cttcagcttc ggaattgtac agattgtcga caatgactac cccgaatccc
     7261 tcattgatca gttcaacgac agtatgggac cctatatatc cggttccccc ggtgactaaa
     7321 attttttctt tcattaactg aaggtgcaag taagtatgat tttcaattct ctccttgagt
     7381 atactctttt attgtcaact actattccat tttcgttaaa cgggaatgat gcatacatat
     7441 tttatatatt ccttgcggtt cacagccagg ataaccaata atatttaccc tcgaaagcta
     7501 tttccagatc aggtccgttt tctccttcat ctcacgcgtc cagacatttg gatccattat
     7561 gtcaatatct gttccggaat catccatttc cttgattcag tcatacatct ccagactcct
     7621 tatgttgata atatcttctt tgaaaaaact tccattttca gagctaatga atgatgtgtt
     7681 ctcctttccc gtgtttaccc tcttgcatgt aatgaacctg ctcaaataat tttatggaaa
     7741 gcggcccaaa cctatcagag tgtgttttgt ctctttttta tagtttatcg tacattttaa
     7801 agattattat gtgcttgcaa taatggatga tattctccta caagacctac aggttttgag
     7861 ttacgtattg taaaggctgt tattatcgac tgtcgcgact cattaaggcc gaagcctctg
     7921 ccggcaagaa tttccctgta agttgccgta tgcagatcac caaatccttc actgaactct
     7981 atttcctcac cttcaaccgt tattgatctg taagtccgct ttcctgactt ttttgcctcc
     8041 gcgggcaaat cattatagtc gatactcaga aaccatctta tccgtgcttt ttccagctca
     8101 atatagccgg cagccttatt tggctcggaa acatgcacaa ttattttcgt ggtatcacca
     8161 aagatccagc caagcatatc gaagaaatga attccgatat ttgtggccac tcccccggat
     8221 ttttgaatat ctcctttcca tgagatgtgg taccagttac cgcggctcgt gatatacgac
     8281 agatcaatgt cgtaaatctt gtctttcggg ccgttccgga tcttctcccg aagctcggct
     8341 attttcggat gcagacgtaa ctgcagtact gtgtatattt tgcggccggt ttctttttca
     8401 atttcctgga gggcatcaat gttccatgga ttcaggacaa ggggcttctc acagatggca
     8461 actgcctgat gccggagtgc aaaacggata tgggaatcat gcaggtaatt gggagagcat
     8521 atacttacat aatcaatttt tgtacctgct ctttttagtt tgtccagatg cctgtcaaat
     8581 ctttcaaatt ccacaaaaaa atcgctctgc gggaaatatc cgtcaagaag tcctacacaa
     8641 tcgaatttgt ccagactggc gagaagattg ttaccagtat ccttgatagc tttcaggtgc
     8701 cggacagcta caaatcctgc cacaccaatg agtccaaaat ttttcgaaga tgagtccatc
     8761 agttatttta tttccgcaga gggtgcctgt tcaataattt ttgttaccat tccatccttc
     8821 agaatgtatt tctcaccgct ttcgggacat gaagcagtgc cggtgttatc aaaatccagc
     8881 ctgtgtccgt attcactcat ccagcctttt tgtcttgcag gattgcccat aacgagtgcg
     8941 tagggtgcaa catccctggt tactacggcg ccggccccta taaatgcaaa cctgccgatt
     9001 ctgtttccgc agacaatggt tgcattggcc cctattgttg cccccttttc cacaattgtt
     9061 ttaacatact gatcctttct tgcaactgca ctcctggggt ttactatgtt tgtgaagacc
     9121 gccgatggtc cgagaaatac atcatcttca caaataaccc cggtatatac ggaaatattg
     9181 ttctgtacct ttacatttct tccaagcttt accccgggtg aaatgaccac gttctggcct
     9241 atattgcaat tgtctcctat ttccgaaccc gacataacat gactgaaatg ccagatctta
     9301 gttcccctgc caatcttgca gccttcatca attacggctg tctcatgggc aaaatattct
     9361 ttttccatat tcagttattg ttaaaaaact ccagcacaca tccggtaata tattcaagct
     9421 gttcacggtc catttcagtg tgtaccggta gggaaagaac ttccctgcaa agggagtttg
     9481 ttaccggaaa atcattttca ccgtaaccca ggtagcggta agcattctgc atgtgtagcg
     9541 gccccggata atatatcatc gatggtattc ccctttcacc gaggtatctt ttcagttcat
     9601 cccttttacc gccctttact tttagggtat actggtggaa tatatgggag gaaaagctgc
     9661 ttctcagggg aaccatgatg tcctggcact ccttgaaagc tttgtcatag taatcggctg
     9721 catgcctcct ggcttcatta taactgtcaa gatgcctcag cttcaccctt aatattgcag
     9781 cctgaagcgt atccagcctg gaattgattc caacataatt gtaatgatac ctgattttca
     9841 tgccatggtt ggcaatgctt cttatttttc ctgccagttc atcatcatcg gtaaacaggg
     9901 caccgccatc gccgtaacaa cccaggtttt tggaagggaa gaaggaggtt gtgcctatgg
     9961 ttccgatcgt tcctgctttt ttcacattcc cgttgctgaa aataaaacgg gcgcctgttg
    10021 cctgtgcagc atcttcaact acaaaaagat tatggccggc tgcaatgctc aatatacttt
    10081 ccatatcggc gcattgtcca aaaagatgaa ctggaataat tgccctcgtt cggggagtga
    10141 tggctttcct gattgagtca ggtgaaatat tgaaggttcc gggatcagga tcaacaatga
    10201 ccggtttcag gccgagaaga gcaagtacct caattgttgc tacaaacgta aaattagtgg
    10261 taataacttc atccccgtga ttcagcccca gcgacatcat ggcaatctga agtgcatcgg
    10321 tcccgttggc acaggaaaca acatgccggg ccccggaata ttctttcaac tcatcctcaa
    10381 acattccgac ttcaggccct tttataaaag ctgttgattg cagaactgcc tgtatggccc
    10441 tgtcaatttc atcctttaga ttttcatact gggctctcag gtcgaccatc tgaatttcac
    10501 gcatcggaaa cagtttttag ctacgaatat aatatttttc accggctttc ggatgaaaca
    10561 gtttctcact ttcattgccg gataatgttt gaaaactcaa tgatgaatgg ttcaaactgt
    10621 gttaataaca tgcaggcttt ttattaggat gatcatgggt attagtctta attttatcca
    10681 cgttgaaaac atgaacaatt ggatataaaa gcattcataa gggaattgtt gttcagttac
    10741 gattgcgtca ttgttccggg cttcggagga tttataggca attattcgcc tgccaggatc
    10801 gaccggacca tgggtacatt cagcccccct gtaaggcaga taacctttaa cagaaatctt
    10861 gatcacaatg acggacttct ggtaggtaag atttcaatgg ctgccggatt gaattacggt
    10921 gactccaggc ggaatgttga ggattttgtc aggaatgtca ggagcaggct ggcaaaagga
    10981 gaaacggtgg tatttgatca tcttgggacg ttttccctta atcacgaaaa cagtatccag
    11041 tttgaacctg aggccaatct gaactattat ctcggctcct atggccttga atcgtttcac
    11101 atgggccatt ataaaggata tgatgtaagg aaacgcgtca gcaggcatat tgacagggat
    11161 cctgttagaa gaacttcagt tgtgaaaaat cttgtgcgtg cagccattat gattccgata
    11221 gttgcactcc tggtcatcgt acctcttaaa gatcagtaca gcaggaaaag cggggttgaa
    11281 tctgtaacac tgaatcccct ggttactgcc gagtttgaaa ataacagaaa tgccatagac
    11341 aacgaccatt cagcaaatac ccctgcagtt gaagaaatct ctgcaaatat cacaccggac
    11401 gctccggttc ctgagactgg gcctgcaaat gatgaaatcc cggaagttga aacaggacag
    11461 aatgtttatt acataattac gggcagtttc aaatcggaag agaatgcact ttcccatatt
    11521 gatttgctaa gggaggaagg gttcgatccg gagattatac gggcttccaa cggctttttc
    11581 agggtcaacg caatgaaatg cagcaatatt ggaatagcga tatcgaaaaa agacagtctt
    11641 tcaagaaaat tccctggggt ctggataact aaaaagaggc ctacttaata atacttccgt
    11701 taccggtttt ctcagccggt gataccagaa ccaatttccc cccgctgtct tcggccatca
    11761 gtatcattcc tttggattca attcctttta ttttcctggg ctcaaggttt gcaacgatag
    11821 agatctgttt tcctacaaga tcatcgggct gaaaatattc agctattccc gaaacaattg
    11881 tacgggtatc gattccggta tcaatggtga gttttagcaa cttagtcgta cggggtactt
    11941 tttcggcttc caggaccgtg gccgttctta tgtcgatacg cgagaaaaga tcaaatgata
    12001 ccgtttcctt tggaggaatt actgtagtac cggtatcgtt attggtcttc agtgaagccc
    12061 tgagcttgtt tatctgcttt tcaataacgt catcttcaat tttctcaaaa agaagcccgg
    12121 gtttttttat tgcatgtccg ggaggaataa ggtcacttct gcccgcttct gcccagctga
    12181 attttccgca gttcagccat cctctcagct tcttagtggt aaaaggaaga aatggttcga
    12241 ggatgatggt cagattggca gttatctgga gggcaatatt gataatggtt ttaactttct
    12301 caggatccga cttatggatc ttccatggtt ctgtatcagc caggtatttg ttccccagcc
    12361 tggccagatt cattgcttcc ctgagtgcat ccctgaatct gaatgattcg aggctgggtt
    12421 cgatattatt cctgatccgc tcaatttccg aaagtacctt ctcatcgtaa tcgtctgttt
    12481 tgcttcgagc cggtgcgatg cctccgaaat aattattcgt caggacaagg gagcggttta
    12541 caaagttacc cagtatggca accagttcat tgttgttttt ggcctgaaag tccttccaca
    12601 tgaaatcact atcttttgtt tcaggcatgt ttgtgcagag aacgtaacga agtacatcct
    12661 gtttgcccgg aaattcctca agatattcat ggagccatac tgcccagttg cgtgaagttg
    12721 aaatcttgtc attttccaga ttaagaaact cgtttgctgg tacgttttcc ggcagaataa
    12781 acgatccctc cgccttgagc atggccggga atatgataca gtggaataca atattgtctt
    12841 taccgataaa atggatcagt tttgtatcag gatctttcca gtatttttcc cattcagggg
    12901 taagctcctt ggttgcagag atataaccaa tgggcgcatc gaaccatacg taaaggactt
    12961 ttccaccggc tccgtcaacc ggcacgggaa tgccccagtc gagatcacgg ctcaccgccc
    13021 ttggctgaag gccctgttcg agccacgact tgcactgccc gtaaacattg gtcttccatt
    13081 ccttgtgtcc ctcaagtatc cactccctga gccaaccctc atacttgtcg agaggaaggt
    13141 accagtggag agtttccctg agaacgggtt tgcttccgct tattgtggag tgagggttga
    13201 taagttccgt tggactcagg gacgtaccgc atttttcaca ctgatctcca taggcgtttt
    13261 cattaccgca ttgcgggcag gttcccacaa tatacctgtc ggccaggaaa cagcctgctt
    13321 cctcgtcgta atattgctcc gttacttttt cggtaaattc gcctttattg tacagagtga
    13381 gaaaaaagtc cgaagctgtt tcatagtgta ccttgtttga tgtacgggaa taaatgtcaa
    13441 acgcgattcc aaaatcctca aaagccttct tgttgagttc atggtacctg tctacaacct
    13501 ccttcggcgt tattccttcc gtacgggcct taagtgttat gggtacacca tgttcatccg
    13561 acgcacatat tgaaagaaca tcctgacctt ttagcctgag ataccgggta taaatgtcag
    13621 aaggtacata aacacccgcc agatgaccta tgtgtaaggg gccgttggca tagggcaggg
    13681 cagaagtaat caggtgcctt ttaaaacttt tcatgggtga ggagaattgt taattgttaa
    13741 acggaacctg tgttttaaaa aagtaatcct gttttttcat tcttaaaacc tgtcaggctt
    13801 catcatccga atctgattta tcgtttcaaa aaaattgttc aaagataatg tttatttcag
    13861 gacttggatc aggtagcatt atgagttatt tttacatgag ataagatttt attatgccaa
    13921 tgaataagat tgaacctctg ctgctgaaga agggtgataa ggttggtctc atatcgcctg
    13981 catgggccat tgatgaggac aaagccggca aggcagttgc cttcctggaa gaatggggac
    14041 tgaaggttgt tccgggcagg aatttgctta aaagatgggg gccctttgcc ggcacggatg
    14101 aagaacgagc cagtgacctc cagattatga ttgatgatcc ggcggttaag gcagttttct
    14161 gttccagggg aggttacggc acattaagaa tcatcgggaa aacggatttc gcaccgttga
    14221 aaagatcccc caaatggttt gtcggtttca gcgatataac agttattcac atgtggctta
    14281 atgaagtatg cggcattgtc tcggttcatg gagagatgcc gctcaattat tacgaaagcg
    14341 gtaagacaga tgagactttt gtttcactgc acaatgtttt attcggaggc aatacaacga
    14401 tcagctggac gggaaggact tacaggttca gggaaatgac cggtgaagtg accgggggta
    14461 acttatcatt gatttacagt cttatcggca ccagggcaga gcccagaacc aggggcagga
    14521 tactttttat tgaggatacg ggagagtatc tgtatcatct cgacaggatg atttcatcgc
    14581 tgaaacttgc aggcaagctt ggccgtattg aaggtctgat tgtcggcggt ctcgacagga
    14641 tggaagatac aaaaatatca tggggcatta ctccggaaga agtaataaag gatgctgttc
    14701 gtgagttctc ttttccggtt gtttttgatt tccctgccgg tcatattccc gataacaggg
    14761 cgttttacat aggaaggaat gccagaatca cttccgacgg aaaaagagtg tcaatgacat
    14821 ttatctgacc atgggaagct gtttatgatt atccttgagg caggaatttg gctattcgcc
    14881 ccggaggttg acttatctgg ttttattcag gcacggcttt tttctcagaa ggccgaaata
    14941 agtaaattaa ggtccttgga tatcatcccg aatttctgac ccagtgtttc atttgtaaga
    15001 atgccattga aaagatagac tccgttgcgg atccccgggt cattttttag cagttgggtt
    15061 atgcccccgg actctgcaaa attctgaaag agcggattga agatattgct tagtgcgatt
    15121 gatgccgtac gtgccattct tgaaggcagg ttccaggcgg aaaaatggat tacgccatgt
    15181 ttttcataca ccggatcgcg aagtggcctg cattcggttg tttcgataca gccgccgcga
    15241 tcgatgctga gatcgataac tactgctcct tttttcatgg ttttcaccat atcttcggtg
    15301 atgtaatatc tcggacgcag atcttccagt tctatggcgc ctataagaac atcggccgat
    15361 tttaatacct ttttcagaac ctggggatgc agaactgagg tttgaagcct ttggccgatc
    15421 ttgttctgga attttttcag gttaaaaagt gatttgtcga agatcctgac ctgggagccc
    15481 agacccaggg ccgcacgggc agcatattcg ccggcggtat tggctccgat tatgacaacc
    15541 tctgtcggag tgacgccggt tattccaccc agcaggactc ccttgcctcc aaggtggttg
    15601 ctgaggtaat ccgatgcaac cagaatggag gttactccgt ttatctcgct catcgattcc
    15661 acaacaggca taacgtcatt actgtccttg attttttcca gggcgacagc tgtaaccttt
    15721 ttccggataa gcttttccag tatctcctca ctgagcgcga gaacattcag gaacgacata
    15781 accagctgat cacctttaag ataatcgact tcctgaagag tgaaaggtgc tactttgatc
    15841 actgcgtcag cgctgaagac tcgttcaggg gaatctgtaa ttacagctcc gttttcgctg
    15901 taatccttat cagaataatt tgcaccggta ccggcacctt tctgcacaat gacttcattg
    15961 tcgttttcca cgagaatatt cacggcttca ggtgtcaggg ccactctttt ctcatcattc
    16021 ttcctgtcgg acggtatacc tatcgttatc cgcctgtgcc tgacagccgt ttcaagctgt
    16081 tcctccttcg gcattaagga agcgtttccg aaactgattc tgccgctctt gtttttaata
    16141 ttcatcatga gaaaagggtt atttcatcaa agttaaaaaa attgaaatta gaagcaaaat
    16201 cagttcattt catgtaagtt caagtatcct ctgttcattt tctccgacag ttatcttaat
    16261 ccttgcggta tcggcaggaa ggatttcttc tatctgttcg ggccattcaa taaagcagta
    16321 ggctccgctg taaaaatact cttctatccc gaaatcgtat gcctcccctg ttttctttat
    16381 cctgtagaaa tccatgtggt acaggggatt gcccgatgtg gttttatact catttatcag
    16441 ggtgaatgtc gggctcgtaa tctgatcggt tgcattaagt gagcggcaca cggcctttat
    16501 tattgtagtc ttacccgcac ccattggtcc gtagaatgca aggattcttt ttccctttgt
    16561 gtattcaaga agtttttttg cagcactctg aagttgcctc ctgctttcaa tgattattct
    16621 catcactggc ccccccggct gttatgacac tggctccagg caggctacgg gaagcattat
    16681 ctcctgcatt gagatgccgc cgtgctggaa agtatcctta tagtaacctg cataatagtt
    16741 gaagttgttc tggtaaacca ggtaatcctt gttaagggca aaaatatatt ttgatgtaat
    16801 gtttgttttt ggtaatccgg ccttttcagg aaatctcacc tcaaaaactt cccggggatt
    16861 gtaatccaga ttccttccca ttttataccg tagattgaat gaggttttac ggtcacctat
    16921 gacctttatg gggttctgta ccctgatggt gccatgatcg gtactgaata tgatcctgac
    16981 attctgtgag gaaagggtaa caagaagttc aaaaagaggg gaatgtacaa accacgacct
    17041 tgtcaggctg cgataagctc tttcatcatt tgccagttcc ttgataaaac ctatctctgt
    17101 cctggcatgg gagagcatgt cgacgaagtt atagacaaga atatttatat cattgtccat
    17161 aagttgcctg agcttgtcat ttacccgtct tccctcgaga ttgctgctta tcttgttgta
    17221 tgaccatttg tacctgaatc ctttcctggt aagctgatat ttgaaaagct cctcctcata
    17281 attgtttttt ccttcatcct gatcatcgtc tacccagaaa tccggtaata tggcagctat
    17341 tgccgaaggc atcagtccgg caaaaatggc attccggctg aactgggtgg cggagggaag
    17401 gatgctgaaa tatatatcct catcaataat cctgaataat cccgataatt cggcggaaat
    17461 ggttttccac tggtcgaaac ggagattgtc aatcaatatg aaaaaaagag gccttccctg
    17521 ttcgacattt gggaagatct tttttgtaaa caacatgggt gaaaggaccg gtttgttact
    17581 cgcctccggg tcaagccacg acaggtagtt actggttatg tatttaataa atgcattatt
    17641 tgcatcattc tcctgcatcc ggaggatttc actcataccg ggatctgttg atttctccag
    17701 ttcagattcc cagaatgtaa ttttcctgta cagctcggcc cagtcggtat gtgtctgcgc
    17761 tgaggatatc atgttcccga tccggctgaa ttcctgacgg tagttgatag ttgtcttttc
    17821 cgtaaccagc cttttttgtt cggtgatcct ttttattgca agcaggatct gattcggctt
    17881 tacgggtttg ataagataat cggcaatctc ggatccgatt gcggcctcca ttataccttc
    17941 ctcctcgtgc ttcgtgatca taaccacagg tatatcggtg cggatttcct taatgagcct
    18001 cagcgtctct atcccgctga taccgggcat attctcatcg aggaaaatga ggtcataagg
    18061 gttttgcttt acaagatcga tggtatcggt accgtttgag caggtatcaa tctcatagcc
    18121 cttgtcctta agaaagaaga tatgcggctt taacgcttca atttcatcgt ctgtccacaa
    18181 aattcggatc tttctcattg ctctttccct ccggaataat tatctctgaa gaagatcagg
    18241 tatcgcccgg ggtccttgtc ttctccaagt atctttaggt ttgtactgtt tatcaggaag
    18301 gtcatgaacc ggctcgaagc aatgtttctg atgatcgatg cagcatcaaa cgcattgaaa
    18361 tacatccggg cctcattgtt gttcctgaaa cccgataccg taatcatcag gtaagcatta
    18421 ccgaccagtg tcccttctgt cctgtaattc tcatttgtaa aattatcaat attatggctt
    18481 ataacatcaa aagttgcctg atttaaatta aaagccggat ttgagataac aagggcgaat
    18541 gtcaggatgg aaattgtatc acggacatac agttcggttg ctatttcctt gtcttcttcc
    18601 agtttgagtt ccgggcgttc ctggttcaga tgagtcatga gctcttcggc ttttttgctt
    18661 tcgggtgaac ccggccatgt tttcacgatt atgctgagat tcgtccggaa agttttttcg
    18721 tcacttattc ttgccgttga ataggcccgg aggagcatag ccttgggagc caggtcatct
    18781 tcgggatatt ttttcaagat ggaatcgcat gtgctgacag ccagggcgta attttcaagg
    18841 gtataggcat caaaggcatt cccgtagagc ctttccgcct ctttgatgcc cgcgagtttg
    18901 ttgttgtaat aatcagggtc ggaaaggatc ctcgcaaatt cgctcccagg atatttttcc
    18961 agtagtcgtt gccggtatgt ttctgatttc tggctgtttt catccttgtt tatcctgtaa
    19021 agctcgtaga gtgtttccgg aacaagttca tgatcgggga accgggagat cagcgtttcg
    19081 aatgattgta cagaatgggg cctgtcggct atcctttcca tgaagatacg tccggaatta
    19141 aaataggcat ttgctatttt ctggttggat atggaaagga gagaatcgtt aagcggaaga
    19201 tccttaagat agaactcagg tttatggtaa tcgctggcgg ccatggttgt gtcagttttt
    19261 ttgaggtcgt tgtcttcctg gcctgatgcc atttgttgta cgttcacccg tgccttgttc
    19321 atcctccgcc agttatcttc gagcctgcgg tcgccccatc ttctccggaa ttcggttctg
    19381 ccaaaggtaa gggcagcctg attgtagaag taccatctgc cttcctggtt tatgttatct
    19441 tcaaatcttc tctggttctc ataaaactgc cccatattat acctgtcaga atattccgat
    19501 gatctgcctt ccctttcagc taaatttatt ttctcaatta tgccggcaat gagggcatcc
    19561 cgctgcgcct ggggcagggc tgcaacgttc tggagactgt cttcggtgcg gataactgtt
    19621 agttctgcaa caagagcatc cagatccctt gattttgtca tcagttccct gtagtcgggg
    19681 tatttctggt caaggaaaaa tacagcactg tcatagtatt ttcctgcagg tataaaatct
    19741 gatttcccga aaaaataatc ggccagggca agataggatt tacctttctg gttattgttg
    19801 ccggtatttg atgctgcaga tttcctgtaa gccttgattg cttcggcttc attgccctgt
    19861 ttcatcttca ggttgccgag tgcataatag atctggtcgt gaaaatcagt attcttggca
    19921 tccttcagca tcttttcaag ctccttgcct atttcctgct gattgccgga ttcaacatca
    19981 aacactccgg caatgttgat gcgggcatta aattccacat catatggcgg attcatctta
    20041 acaacggccc tgtaatatga agtggccttt ttcccgtcac caagtcgttc atagagctgg
    20101 gctagcagat aggtaagcct gtatttctgc gttttgccgg acgagttttt tatggattca
    20161 ctaagcggag aaacagcttc ggagtagttt ttttgtttga cgtacaggtc tgccagtgtt
    20221 gaataataca gggactttaa cgattttggc gaagaaggtg tgatttcgat ctccctgagg
    20281 agcctcagcg agttgttgta atcgccggtt tcattgccta ttcttgcaag ccagattgct
    20341 gattctgttt tgatcccggg atcgttggct tcggcaatgc agtagttaaa gacggaagca
    20401 gcttcgttgt attcctgttt gtagaaacgg gcttttgcga tcaggaagta gctgtcgtca
    20461 acccattcgt tgaattcctt cttttcgagc agtgattttt cgctttccga caggtttctc
    20521 ttgttgccca gttcgggtct ggccgttatc gacttcaggg agatcagttt ggatgctttc
    20581 tgtatagccc ggtccatatc cgaggaacaa agctgtactg ttgccggatc gctgaattca
    20641 aaaactttaa ggagttcgga aaaatcatct ttatagcctc tgttgatctt tgccaggccg
    20701 gccttaaagc tttcgtagcc gttgaaatat atgttgtacc gcgaagtaag gccctggtag
    20761 aatcttgttg tggctgtgtt tttttcaacg gaacaacccg ttaggatcat aaacaatcct
    20821 gacaatacca gatatttata tgaacttctt cccggataca atgactttta acacttaaac
    20881 tgatggtaac cgtgtatagt atccaaaggt agttaaaatt ggaatgatcc tgatggtcag
    20941 gctgaaataa ccttcttaac ttccggaacc tcctgcatta ttgcctgttc aacaccggcc
    21001 ttgagagtct ggaggctgaa ggggcatcca tggcatgctc ctttcagcct gacttttaca
    21061 accatatcat cggtgatttc cacaagctcg atgtccccgc cgtcattctg aagatatggt
    21121 cttactctgt ttaaggctct gactatacgt tccctggttg catttgaatt ttccatttga
    21181 cagtattgtt aatttaagat atctgattgg ttgatggtca tctccgctgc tggtatgtca
    21241 cctcatttat tacctgttca ggcctggcgt caggtgacac tcacttttac cgtcggtttc
    21301 tggtccctgt ttctcttttc cactttttcc acaacctgtt ctgccagctt catgaatgcc
    21361 tgagcggaaa tggtttctcc gagggcaaca ggatcgccgc tgtcactacc ctcacatata
    21421 ctttgtacaa gtggtatctg acccagaagc ggaacattca ttttttcggc aagttttctg
    21481 cccccatcct tcccgaaaat atagtattta ttatccggga gttctgcggg tgtgaaccac
    21541 gacatgtttt cgacaagtcc gaggaccggg acattgatcc gtgcactgcg gaacatcgtg
    21601 atccctttga tggcatcggc aagggctacc tcctgggggg tggttattac gattgcacct
    21661 gttacagcca cttcctgtac catggtcagg tgaatgtcac ttgtaccggg gggcaggtca
    21721 ataaacagga aatcgaggct gccccattct ccctgtgcca tcagctgttt aaggaaattc
    21781 gatgccatcg ggccccgcca gacaagtgca tcggacgggt caacaaaaaa tccggtcgac
    21841 aggattttca caccatactt ggtgagcggc acaatatgat ccgtgtcatt ccttctgaag
    21901 acttcgggct tgtaattctc ggcattgatc atttttggta ccgagggtcc gaaaacatca
    21961 gcatcaagca ggccaacgct gaatcctttt ctcgcaaccg atattgccag attgaccgca
    22021 atggttgtct tgcccacacc gcctttaccg gaggaaactg caacaatgtt cctgactccc
    22081 ggcagaagac tgtcactttg tttttcagtc tcatgaacga ttgtcctcgg ctggatgata
    22141 atattgctta caaccgcatc cttcccgaga tcatccttga ttgccctcac aatcgagctt
    22201 ttgacggaag aaataaaagg atcatttgat ttttccggtt tgagggtaat ggagatacct
    22261 tcattgccgc tttcaatttc agcgactatt ccaagcgcca caatatcctt gcctttttcc
    22321 gggtggatca cttttgtgag cgcttttaga atcagctctt ttctgaacat attattattg
    22381 atctgttatt tttatttaag tcaaattaaa ataaggtgca attttacgat ttttccgggg
    22441 gatttcaaaa atcggttagc tcagatgacg gatcccagaa tttttcattg aaattcagga
    22501 taatatcatt ttcaatgatc attccttcat tctcaagtaa ctgtctcatt gttgatgaat
    22561 tgccgaagtg atgttttccg gtcagcattc ctttacgatt gactacgcgg tgggccggaa
    22621 taaattccga ccttccccct gaagcattca gtgcccatcc taccatccgg gcagatctcc
    22681 cggtcgccag gaaccgtgca attgcaccat aggtcgttac cctcccaggt ggaatgagtt
    22741 tggttacctc gtaaacgccg gagaagaaat cattatttcc ttgtttgtta ttgctcatcc
    22801 ttcactattg tatttttatt cagtttgaaa gccagataac atatcttcaa acctttatcc
    22861 agaaacatct tttcataatg agttctcagc gaaaaaatcc tttcaggaat catttcatcc
    22921 gttccataaa ctccaatgcc aggcaggaaa ctgaaaaacg ggacgctaac atcaggattt
    22981 ccaacataca tatcatctgt ggatctgaga atctccaatc cgttgttttt cacaagtttt
    23041 tcagtgtacc ggaacagttc ctggttatcg gtttttagat ggatcaggcc tccgtctttc
    23101 atgagaagcc ggtacttatt taggaaccac ggacttgtta gccgtttgtt tgagtttctt
    23161 ttcctattgt gcggatcagg gaacactatc catatttcat caacttcatc tttggtgaag
    23221 aatgaattaa tgaactcaat tcttgttctg agaaaggcag tatttttcag tcctttttca
    23281 cttgcggttt ttgcaccccg ccacatcctg gctcccttaa tgtcgattcc gatgtaattt
    23341 ttttcaggga agatcgtggc cagacccagg gtgtattcac ccttgccgca tgccaattcg
    23401 agtacaacag gattattgtt gccaaatatt ttgtcgcgcc acttgccctt taaaggatga
    23461 tcttcaccgc tttcatgtcc ggccccggct tggatcacgt tcccgaatgt ttccagttca
    23521 gcccatcttg cgagtttatt tttgcccacg tcatcaatca ggtttacgga tcttttcaat
    23581 ccttatgcca aagtcataaa cactcctgag gtccctgaca cgcattccat gactgaccct
    23641 gatatggtag gtgcctttta cggggaataa tacgttggtt ttgtatggca gcaggagttc
    23701 ctttatgtca ccgaatcctt ttccatacca tttccccttc tcatcggcca gataatattg
    23761 cagtgtgtct gtgatgcgtt tttcatcggg agaggttgtt gtaacaaaga gataaatgtt
    23821 cctgtacgga tatgcggtac cggtccggat ggtaaaaaag atattgtttg ccgtaattgt
    23881 atcattaaca ggcacatcaa attcggatac attttccaga tcccatgttc ccgagggcat
    23941 ttttgaggaa tcggtaaaaa cgatatcacc tgtgcaggat gaggttatga tcagaagcag
    24001 tatggctccg gcaaagaaaa gtttatttcc tggcatttgg ttttttgttt tttctttttc
    24061 gcgcggactg tcgccgatgg gtatttttgg tttcggaagg atcaaacctt gtaatgctgt
    24121 tatccataag gagatctttt gaatggatat ccggattgtg gaattcatca gggatcagca
    24181 gtttgtcggg cttctttcca ttcctgttca tcatttgtat ttcccttacc cttgatatgg
    24241 gaactgcagt caggtctacc ggggaatgcg gatctgatga ataccagtaa atattcttat
    24301 ggacttccat tttctggtag tatgcctttg aatttgccag ttcaagagga atatccctgg
    24361 gcgggaaatg cttctgggca tcgagataac agtcgaattc aaagttcaga cagcatttca
    24421 gtttactgca ttgtccggca agcttttgtg ggttgagggt aagttcctgg tatttggcat
    24481 gggttgttgt aaccgacatg aaattcgtaa tatgcgttga acagcacagt tccctgccgc
    24541 atgccccgat gccgccgatt ctgccggctt cctgccgggc gccgatctga cgcatttcga
    24601 tccgtaccct gaactcctcc gccagtacct ttataagttg cctgaagtca accctttcat
    24661 cagcaatgta gtaaaatata gcttttgtct tgtcaccctg atactcaaca tcaccgatct
    24721 tcatgttcat tttgagctct tctgagatct gcctcgatct gagcattgtg ggtatttcaa
    24781 gttgttttgc ttcttcccat ttcaggatat caacaggttt tgctttacgg taaacttttt
    24841 tcagatcgtc agttgacgga aggatcttaa gctttttaag ctgctcaatt atcagcggtc
    24901 caaccattga cacccttccg atatcgtgtc ccggtgatgc ttcaactgca acaatgtcac
    24961 caatctctag cctgaggtta ttaatgtttt tataaaaacc ctttcttgtg ttcttgaacc
    25021 tcacctcaac cagatcatat tccgcctggt tgtagggaat atccttaagc cagttgaatg
    25081 agtcaagttt attgcaaccg ggcttgtatt tcgtgtccgg cagatggatc tcttcaccat
    25141 gctgtccgga tagttcttcg ctttccgtca ttgggatggg ttaaatagaa taattcaatc
    25201 cggcaaaatt agtaaatccc gcagtttttt caaatacagt tattactgtc tgattagacg
    25261 tgtcagctta agtgcaaggt caaggaatat tatttttgca tttccgtttg cctccacatg
    25321 tgcatgtgcc aggttcagtt cctctgaaaa gccatgtaca tttttctggt ttatgaaggg
    25381 atggaacttt gatgaaaaag ccgtctcatc atccgtcaga tatacaatgt tatttttccc
    25441 ctgaccaagg gtaagcataa aattctccct gaccagtctc agggcgaagg ataggaaatt
    25501 cttttggggc tcccggccta ttgatgccat ttcctcagac caggaaatga tagagatgat
    25561 gtctcttttc catgcaaatc tcataaggtt tttaaagcga tccaggttga gaacagttgt
    25621 gtcctggttt ttacacatct cgaccgctct gacgatattt ccgtttgcaa cccttgcaat
    25681 atcgtttgcc tgtttatccc cgatcccgaa attggactga aggtaaccgg caatatcgga
    25741 caacctgaat gatggcacct taaccatctg gcatcgtgaa gcaattgtcg gaatgatctt
    25801 atcgggttct tcggaaacca gtaaaaacag tgttttctca ggaggctcct cgatcatttt
    25861 tagcagcttg tttgccgtgg cgatatgcat tttctcaggc aaccagagaa tgaggatttt
    25921 ataatccgat tcaaaggttt taagcattaa ctttctgata atttctgaag cttcagacgc
    25981 aaaaatcaat ccctgcgcat ttccgacctc aatgaaatcc agccagttgt taagcgagaa
    26041 aaagggtgac tgcccgacaa attccctcca ttcagaaata taattgtcgc ttacctgctc
    26101 ttttgtcttt ttatccttta taacgggaaa cacaaaatga aggtcgggat ggatcagttt
    26161 ttcatatttt aagcaggact tgcattttcc gcatgaatcg gaaggcaacc tgttttcgca
    26221 gctgatgaac tgggcataag ccagggaaac agcaaaactg ccgcaacctt ccggacctgc
    26281 aaagagttgt gcatggctca ccctgccctg atccaccgtt ttaaccagtc cggccttgat
    26341 ttcctgctgc ccgggaatat ctgaaaatct cataatcagt tttattcttt tgaatgcagt
    26401 aatgtgagaa caaaatttcc ccgtgatggg aacctgcctg attaaatttg ccggcttctc
    26461 aatgtaatcc gggttattgc atatggctca aatttaatga atcttttaat accatgaaat
    26521 gtggtaccgg agcatcggtt caatgcatgg aaatgcttat attcccgtga ttggcggttg
    26581 acggatttac ggatttataa aatcagggga cctgattgtt tttaattctg caataacttt
    26641 ttacacgcgc aaaagatatt tccaaaaaaa atgtaggttt gcatcaggat attacaaaat
    26701 ttaaatactc tatttatgaa aatgattcag gactttgatt ttaaaggggt aaaggcaatt
    26761 gtaagggttg acttcaatgt tcctcttgac aagaagactc ttgttgttac cgacgatacc
    26821 aggataaggg gagctattcc gacactaagg aagattatcg gtgacggggg ctccgttata
    26881 cttatgtcgc atctgggcag acccgagggc aggatggaaa agtattccct gaagcctgtt
    26941 cttccggttc ttgagaagca tctcgggcaa agcgttaaat tcgctgatga ctgcctgggt
    27001 gaatcagctg tttcaatgtc ggccgctctg aaaccgggag aagtcctgct ccttgagaat
    27061 gtaaggtttt atcccgagga agaaggaaag cctgtattgc ctgagacggc tactgatgaa
    27121 gagaagaaag cggccaaggc tgagcttaag ctgaaacaga aggaaatggc caggaaactt
    27181 gccggctatg ccgatgtgta tgtaaatgat gcttttggca cagcacacag ggctcacggc
    27241 tcaactgctg tcattgccga ctttttcccg aaagataaga tcatgtttgg atttctcatc
    27301 aacagcgagc ttgctgcgat ggacaaggtg cttaataatg cccagcgccc tttcacggcc
    27361 ataatgggag gggcaaaggt ttcggacaag atcctgctta ttgaaaatct gcttaacagg
    27421 gtggataatc tgattatagg aggcggaatg acatatactt ttgtcaaggc aaagggtggg
    27481 aaaataggaa aatccctgtg cgaggaggac aagcttgatc tggcattgaa gatcattgaa
    27541 aaagcggggg aaaagaatgt gaacctttac ctgcctgtgg acagtgttaa cggggataag
    27601 tttgacaatg aagccaatat aagggttacg gcggttgatg ccgttgatga cggctggatg
    27661 ggtctggaca ttggagagaa gaccattgaa gaattcagcg gggttatcgg gaaatcgaag
    27721 acaattctgt ggaacggccc tatgggagtg tttgaaatgg agaagttttc gaaaggcaca
    27781 acagcaattg caaaggcaat tgcttcagca acttccaaag gggcattcag ccttatcgga
    27841 ggcggagact ctgttgcagc gataaacagg aacgggcttg ccgacaaagt cagctatgta
    27901 tccaccggcg gcggagccat gcttgaatac atggaaggta agaaactccc ggggattgtg
    27961 gcaatcaggg gagaatagcc ccgggtacca cctgagcaaa tcaattcagt ccggatccgg
    28021 actgaattga tttgctcagg tg
//
LOCUS       DAOOYG010000023        27857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1489_length_27857_cov_6.346126, whole genome shotgun sequence.
ACCESSION   DAOOYG010000023 DAOOYG010000000
VERSION     DAOOYG010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27857
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1489_length_27857_cov_6.346126"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PK288_03685"
     CDS             complement(<1..275)
                     /locus_tag="PK288_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPM18053.1"
                     /translation="MRYFIIAGEKSGDLHGANLVRELIKADPAAEVECWGGDLMEASG
                     AKLLMHYKNTAYMGFVEVLKNLRGIRKNISLCKSQIISSGPDLTFAQ"
     gene            complement(272..571)
                     /locus_tag="PK288_03690"
     CDS             complement(272..571)
                     /locus_tag="PK288_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18054.1"
                     /translation="MTGTGKYDKTPVGALAGFILPLVAAFLFWFFSPGDLSIPSYFRK
                     IIRADILTHIMSLSVTCNLVLFLVFNRLDMLQASRGVLGMTIAWAVLVFTVKFLL"
     gene            complement(568..1350)
                     /gene="surE"
                     /locus_tag="PK288_03695"
     CDS             complement(568..1350)
                     /gene="surE"
                     /locus_tag="PK288_03695"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPM18055.1"
                     /translation="MVVEKGDRLILLTNDDGLYAPGLRALLEVMEEFGKVILISTVEG
                     MSGMSQALTVKTPLRVKLLEETGSRKVYACNGTPTDSIKLAVNQLADKKPDWVVSGIN
                     HGSNASVSVLYSGTMAAALEGCLYGIPSVGFSLSSFSKTADFSPSKKFIRIIMMHLVN
                     ESLPDGICLNVNIPALPEDQIRGIRVCRQARGNWKEEFEKRRDPMGKTYYWLTGMFNN
                     HEPDATDTDEWNLSNGYVSVVPVSVDMTAYGYIDTLKEKFGK"
     gene            1526..3982
                     /gene="pheT"
                     /locus_tag="PK288_03700"
     CDS             1526..3982
                     /gene="pheT"
                     /locus_tag="PK288_03700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPM18056.1"
                     /translation="MKISYNWLKDYLATDLAPQKVAEILTDIGLEIESIEEWESVRGG
                     MKKIVVGEVLTCTRHPGADRLFLTTVDVGSGEPLRIVCGAPNVATGQKVAVATEGAVI
                     YQDEKVIEIKKSKIRGEISEGMICAEDELGLGNDHTGIVVLDINTKPGTPAAEYFNVI
                     SDTVFVIGLTPNRIDSGSHYGVARDLAAYLSLNTGKTFKARLPSVDDFKPDNTNNTYD
                     IIVENPIDCPRYTGITISDIRVGESPEWLKNRLKAIGLNPINNIVDITNFVQNEIGQS
                     LHAFDADQIRGKKVIIKNLPDKTKFVTLDETERSLSSRDLMICDIAEGMCIAGVFGGI
                     KSGITSESRNVFLESAYFNPVTIRKTSKRHGLQTDASFRFERGADPNITDWALKRAAM
                     LIRDIAGGNISSEIVDIYPNKIQNVLIEVNYSNINRLIGKKIEKSTVKRILELLEIIV
                     RNEENDSLLLEIPAYKVDVKCEADVVEELLRIYGYNNVQFSSHVNSTLTYIEKPDKEK
                     VVNTISDLLSDNGFTEIMCNSLNPASWYENNPDFNSDTLVRLANPLSSDLNAMRQSLL
                     FGGLNSVVWNIHRQNPDLKLYEFGNCYFRKDNIHGFPEADDYIEKTSLDLFITGNFRR
                     ENWNQKTNPSDFFHIKSATEMILIRLGIKPEMLTAGESDKSYFSESASYFFNNMLVAE
                     TGMISRNYLTYFDIAQDVYYTHFEWDMIMELIRNSRIVFRELPRYPTARRDLALLVDK
                     EIRFAQIRELAFKTEQRLLKEVGLFDVYENESLGHNKKSYAVSFILRDDDQTLTDRSI
                     EKVMNNLIRVYEKDLNAKIR"
     gene            4021..4902
                     /locus_tag="PK288_03705"
     CDS             4021..4902
                     /locus_tag="PK288_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HPM18057.1"
                     /translation="MENIIKRIATYLSLVKFSHTVFAMPFALIGFALAVSDRNHVFSV
                     KLLVLIILCMIFARSAAMGFNRLVDRKYDSLNPRTLGREIPAGKVSPSSATVFVVANS
                     LLFIITAGFINRLTLILAPLALTIILGYSLTKRITWLCHFILGLGLSLAPIGAYISVT
                     GQFSLVPLIYSLIVITWVSGFDIIYALPDEEFDRENRLFSIPAAAGKKKALVISSLIH
                     FATFILVIFAGLKSMGGIFYWIGAATFTALLAYQHMIVRPDDLSRVTHAFQTTNGYAS
                     ILFATFTILDLIIRHPA"
     gene            4932..6068
                     /locus_tag="PK288_03710"
     CDS             4932..6068
                     /locus_tag="PK288_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18058.1"
                     /translation="MLVNMDHIIVREVLSRSELKKFISLPHKIHKTEKNWLPTMYTDD
                     RKLFSKSRNRYFRHYHSILLLAYRDRIPAGRVVALVRKDPDAETDRSCRFCYMDAYDD
                     PQVVHALVERIEIWAREKGADRIIGPLGFSIKDPHGFQVEGFGYPPIIATANNSPWMP
                     RLIENEGFEKDIDLVNYVIDIPEEYPPFYRKAFERILGNRKIRIVEFRNRKDIKPYII
                     QGLELMNETYSEHYGYISMTDEDKQEFAREYLPVLDPEFIKIVEYDGEIVSFIIGLPD
                     FNQGIIASKGRLLPLGFIRILRSFKRSKKLIFVLGGIKKSFRGMGLDILMAVRMFESG
                     LKHKMEFIDSHLIVESNQKMRAEFEIFNGKLIKRFRIYKKTIRT"
     gene            complement(6058..7812)
                     /locus_tag="PK288_03715"
     CDS             complement(6058..7812)
                     /locus_tag="PK288_03715"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPM18059.1"
                     /translation="MRGKKLLPLFVLICVTCIPPALPQVKNIVDNTDLRIIDSLNARG
                     LLMVSDTGRREISLSYDQALRFLQKSYQPHLWKNIRDPFRSAIGQVIFEASNPPFDSA
                     RTYLMKYPYDSIRIKRDEFYIWEPVRFRVPVKVSPDISAEQPEETDTVRLAAKAETVV
                     MKDTTLMVVVDTLDYVTSSHRDFPFRYYRYPFQGDSLKAAVSVLLDAIEARDSSIVSI
                     TGMYSGKTNIWLNSKSDLMVRYWLKNDLNDSVTVWIGSPGRNTIGLYLEQGVIFRRPP
                     MQGNISGARVNVEQQDKTKLQDIRKIVTKAQLWKYRTEAILALNQGLLSNWVKGGESN
                     ISTAFDVTWYADYLNKPYLVSSNNFVRLKYGLIASTHNGVRTNIDLFETNSKLNHKAF
                     GKFDFSATALFKTQITKGYDYPNDSIPVSKFLNPATLTLGLGLDYKPNKLTSINLSPL
                     SYKGTFVPDTSMIDQTKYGVAKDRRSKHEPGASLLVSHEFKPLKTVSVKNRLQLFTNY
                     IHNPQNIDVDWEMIVVASLNWFTDIRLNTHLIFDDDTRTLLFTRGKDPVLGPDGLQKK
                     TARIQFKELLGLSLAFRF"
     gene            8120..9466
                     /locus_tag="PK288_03720"
     CDS             8120..9466
                     /locus_tag="PK288_03720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPM18060.1"
                     /translation="MHDLTTGKEGKLIFQFAAPMLLGNIFQQLFSVVDSIVVGNYIGK
                     TALAAIGASFPVIFMMISMIIGLVMGTTIVISQYYGAGDLVKVKRAIDTMYIYSFIAG
                     TIATVAGITLAEPVLRLLALPEEIMPQAKQYLRIYFCGMLIFFGYNGTSAVLRGLGDS
                     KTPLYFLIIGTIANIILVILFVGIFRWGVAGAAYSTLLANGLAFLLAIIWLNKTHKLI
                     RIAIRGLHFDREVFRQSLRIGLPTGLQQTLIAFGNLAVLGIVNTFGTNVVAAFSVAYR
                     IDSLAIIPAMSFAAAFSTFVGQNIGANKTDRIRTGLISAIKMSGSVTIVTTILIIVFG
                     NLLMNLFTSDQEVIKIGNQYLTIVSSFYLVFALMFIYQGAMRGAGETMIPMLFSLFSL
                     WLIRIPLAYFLSRKTGATGIWWATPVGWIFGLAFSYAYYKTGRWKKKAVVKYDQNL"
     gene            9492..10367
                     /locus_tag="PK288_03725"
     CDS             9492..10367
                     /locus_tag="PK288_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPM18061.1"
                     /translation="MIRSMTGFGKAIAETPQKKITIEIKSLNSRQLDLSTRLPMIYRE
                     KEPEIRNLVSQKLDRGKIEFSIYLDILGDESIPLISKSTVGNYYKQLREISSELDINA
                     GEQLLPVIMRLPEVLQTERAELSEEEWELAKKQIVESLEMVNSYRIEEGKSIEKDIRG
                     CLARILESLEMIGDFEPDRITRVREKLLSILEGNLLTENIDRNRFEQELIFYLEKFDI
                     NEEKVRLRKHCEYFVQTIDEDAPNGRKLNFIAQEIGREINTIGSKANDASIQKLVVMM
                     KDELEKIKEHTNNIL"
     gene            10385..10951
                     /gene="gmk"
                     /locus_tag="PK288_03730"
     CDS             10385..10951
                     /gene="gmk"
                     /locus_tag="PK288_03730"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPM18062.1"
                     /translation="MKGKVVIISAPSGAGKTSVVNHLLGMDLNLEFSISATTRDPRGS
                     EQNGKDYYFMPEHEFRLRIKNDEFLEWEEVYKGQFYGTLKSEIERIWSHGKHVLFDVD
                     VSGGINLKNFFGTNAISIFIMPPSVAELEKRLHIRGTDEKAKIRIRVEKALEEIRLAD
                     RFDNIVVNMNLGVAQQEVYAIVQSFLDN"
     gene            10958..11554
                     /gene="nadD"
                     /locus_tag="PK288_03735"
     CDS             10958..11554
                     /gene="nadD"
                     /locus_tag="PK288_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPM18063.1"
                     /translation="MSKIGLFFGSFNPVHIGHLAIAGYMKEFTDIDQVWLVISPQNPL
                     KKKGTLLADHHRLYLAELAIGDNDALKPSDIEFRRQGPSYTIDTMALLSEKYPRHRFV
                     LIMGEDNLYTLHKWKNARELVKRYPILVYPRPGISRKENIQLDEILSMATIRRVNAPL
                     MDISGSFIRNGIRRGKNMSYYLHPAVWQYIVYMHFYEK"
     gene            complement(11639..12205)
                     /locus_tag="PK288_03740"
     CDS             complement(11639..12205)
                     /locus_tag="PK288_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPM18064.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKIMAYAGVLQDMEVSWMPSYGPEMR
                     GGTANVIVILSTERISSPIIRRFDTGIILNQQSLDKFEGSIKPGGVLVYDGNGISRHP
                     VRNDISIYRIDATEEAARLNSPKTFNMIILGGFLEIKPVLSVENVVNGLKKSLPERHH
                     RLIPENAKALQRGGEMMREVLRIREQDK"
     gene            complement(12222..13013)
                     /locus_tag="PK288_03745"
     CDS             complement(12222..13013)
                     /locus_tag="PK288_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPM18065.1"
                     /translation="MAEVTGIKEIIKPENLVYKRTGIMTDTVLSYCPGCGHGTAHRIL
                     AELIEEEGLQSKAIGVSPVGCSVLLYNFLDIDWQEAAHGRAPAVATAIKRLLPDRFVF
                     TYQGDGDLAAIGTAETIHACNRGENFLIIFINNGIYGMTGGQMAPTTIPGMKSSTSPF
                     GRDVRMMGNPLKMTDIVATLPGTYYVTRQSVHTPANVKKTKRALRKAIQYQFHNKGTC
                     FVEIVSNCPSGWKMTPVESIRWMEENMLPFYPLGDMKVPDEKTGK"
     gene            complement(13017..14087)
                     /locus_tag="PK288_03750"
     CDS             complement(13017..14087)
                     /locus_tag="PK288_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPM18066.1"
                     /translation="MERELRLMKGNEAVAEAAIRAGADGYFGYPITPQSEILEYLMEA
                     KPYETTGMVVLQAESELAAINMVYGGAACGKKVMTTSSSPGISLKQEGITYIAGAELP
                     CLIVNVVRGGPGLGTIQPAQSDYFQAVKGGGHGDYHLIVLAPASVQEMADFVELGFDL
                     AFRYRNPVMILSDGAIGQMMEKVYLNPHRERSKDVPEWATTGKPPTRSRNIITSLDLD
                     PEAQERFNLKLVAKYNEIREKEVRYEEFQCDDADYLLVAYGTSARVCQKSVQLARMEG
                     IRVGLLRPITLFPFPSERISVLADRVKGILSVEMSAGQMVEDVLLAVNGKVTVKHYGR
                     MGGMIPTPEEVVENLRSKIIGG"
     gene            complement(14095..14319)
                     /locus_tag="PK288_03755"
     CDS             complement(14095..14319)
                     /locus_tag="PK288_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM18067.1"
                     /translation="MAKVRGSIVVNIDRCKGCEICMISCPTGTIAMAEEVNSKGYNFA
                     YPANPDLCIGCANCAIVCPDGVITVYKTKI"
     gene            complement(14340..15656)
                     /locus_tag="PK288_03760"
     CDS             complement(14340..15656)
                     /locus_tag="PK288_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPM18068.1"
                     /translation="MNDIPISDNIIRPIMDNMGLKLLGNASIREIVALVNLIESETGI
                     KFIRMEMGVPGLPAPSVGIEAEIRALKEGVAAIYPDITGLPELKKEASRFIKLFLNVD
                     VDPLGCVPTVGSMLGSMLCFLVANRNDRTKEGTLFIDPGFPVQKQQVKMLGHGYFSFD
                     VYNFRGKKLRAKLESYLETGKVSSLLYSNPNNPAWICLNEDELAIIGELSRKYDVIVI
                     EDLAYFGMDFRKNYSNPGEPPYQPTVARYTDDYVILISSSKIFSYAGQRIGIMAISDH
                     LFNRSYPDLLRYFSSDKFGHSAIFGALYGLSAGVAHSVQYGLAALLKAVNDGTLRFGE
                     MLSEYEEKAKIMKRLYTENGFNIVYDKDLDELIGDGFYFTISYPGMSGEQLLSELLHY
                     GISAITLDTTGSERLEGLRACVSKVPMSLLGDLELRLKRFRADHQV"
     gene            complement(15653..16966)
                     /locus_tag="PK288_03765"
     CDS             complement(15653..16966)
                     /locus_tag="PK288_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPM18069.1"
                     /translation="MEIWNRHFECMDRNELRKVQGERLIETVERTYFNVPYYRHRMQE
                     LGIGPESIRSVEDITRLPFTTKQDLRDNYPYGLFAVPLSEIVRIHASSGTTGKPTVVG
                     YTRNDIATWSEVMARTLTCAGADKNDFIQIAYGYGLFTGGLGLHYGGEKIGASVIPIS
                     GGNTLRQIQLMHDFGSTVLACTPSYALYLAEAIIESGISRDEFRLRVGVFGAEPWTDN
                     MRREIEDKLRIKAIDIYGLSEVIGPGVASECLFQEGLHINEDHFYPEIIDPETLQVLP
                     EGSTGELVFTTITKHGLPLIRYRTRDLTRLNYGRCKCGRTFVRMDKCVGRSDDMLIIR
                     GVNLFPSQVESILLEMGGITPHYQLIVDRINNLDSLELKVEVDEAFFLDRISQLEGLR
                     SKIQGTLESYLGLGIKVTLVEPKALERSEGKAKRVIDKREIQVLK"
     gene            complement(16968..17393)
                     /locus_tag="PK288_03770"
     CDS             complement(16968..17393)
                     /locus_tag="PK288_03770"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HPM18070.1"
                     /translation="MLIPQLSVFLENKSGRLTEVLEVLGKEALRITALSVADTSEFGI
                     LRLIVSDTEKAKSLLKDNHFTVNLSEVISVMAPDEAKYYAKILKILSDQEISIEYTYA
                     FSCGSKSIIILRCNNNGQAIKALKDHEMELINASDLYKI"
     gene            complement(17474..19531)
                     /locus_tag="PK288_03775"
     CDS             complement(17474..19531)
                     /locus_tag="PK288_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPM18071.1"
                     /translation="MLNEKLISPASIVVVGGSDDLMKPGGKILKNIIDGGYTGELFVV
                     NPGHDFIQGRRSYRDINDIPGVDLAILGIPAKYCYQVVDVLVRKKNTKAFIIISAGFS
                     EAGEEGRKMEEDIAGLIDEAGGCLIGPNCIGVITRSYQGVFTTPVPRLSERGCDFVSG
                     SGATALFIIESALPKGLRFSSVFSVGNSAQTGVEEVLEYWDLTYDPARSSPIKLIYVE
                     TIPDPDKLLRHASSLIQKGCRIAAIKAGTTEAGSRAASSHTGAIASSDSAVEALFRKA
                     GIVRCSGREELTTVASVFMHKPLRGRNIAIVTHAGGPAVMLTDALSSGGMNIPKLDIE
                     HQNRILAMMNPGASAVNPIDMIATATIDQLGMIIDYADRQLEMIDGMSVIFGNNGLTD
                     MTEIYDLLHRKINECSKPLYPILPSVSSSSKELEDFIGKGHVNFPDEVVLGRALTRIS
                     NTPFPAEEKIFLENIDVRKIREIVDNSVSGYLEPELIQQLLKAARIPVVKEAIARSRK
                     DLVILAEEFGYPLVLKVVGPVHKSDVGGVTLNIKSQRHLEAEFSRMTKITGVTGVMIQ
                     KMLSGTELFIGAKYEPRFGHIILCGLGGIFVEVLGDVSSGLAPLTFNEAGSMIKSLRA
                     YKIIKGTRGKPGINELKFAEIIVRLSSLLRFATEIKEMDLNPLIGSPEGITVVDARVR
                     IEK"
     gene            19946..21049
                     /gene="hydE"
                     /locus_tag="PK288_03780"
     CDS             19946..21049
                     /gene="hydE"
                     /locus_tag="PK288_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPM18072.1"
                     /translation="MGIDRIIEQDAFARNDIIRLLQSEGPDKELLFRKSAEVKEKLIG
                     KKVWLRGLIEFSNICSKDCLYCGIRRTNKNLLRYNLPDEEILSAAKFAYDNRYGSVAL
                     QSGEMESRSVTLRIENLVRKIRELSGGTLGITLSVGEQEPEVYKRWFDAGAHRYLLRI
                     EATNESLYRKIHPHNGRYDFRRRLDCLKALQDIGYQTGTGVMIGLPFQTMDDLAGDLL
                     FMNEFNIDMCGMGPYIEHADTPLAKHSADLPPLSARFDLTLKMIAILRLMMKDINIVA
                     ATALQAIDPVGREKAVRIGANIIMPNITPGPYRDSYRLYDNKPCTSDSAGDCRDCLEA
                     RLSLADHEIVYGEWGDSIHYEKRRKGKQQNPDK"
     gene            21387..22853
                     /locus_tag="PK288_03785"
     CDS             21387..22853
                     /locus_tag="PK288_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPM18073.1"
                     /translation="MEQTIAGSEVKFFNGKQIPVEMHKVRIVQSLNLVPVERRLEAIE
                     EAGYNSFLLSTSDIFLDMLTDSGTNAMSDAQVSKMFVADDAYAGSQSFEKFQKSVLKV
                     FGKKYVLPVHQGRAAENILGKSYVKEGSVVPMNYHFTTTLAHIQLNGGKVLELYTAEA
                     MKIKSTNPFKGNIDINRLEEAIRTYGRENIPFIRMEASTNLIGGQPFSLANLREVRKV
                     ADKYGLKVVLDASLIGENAYFIKVREKEYKNCTLESILLEMSGLSDIVYFSGRKVSSS
                     RGGAICTNDLEIYNQLKYLVPVYEGFLTYGGIPIREIEAMAEGLNETTEVSVVNQSPM
                     FINYMVRELDQLGIPVIMPAGALGCHVDAMQFLSHVPQPEYPAGSLVAAYYIISGIRG
                     MERGTMSNNRDENGVDILADLELMRLAVPRRVFTLSQIEYAIDRLNWLYQNRHLVGGL
                     RFTEEPPILRFFTGRLVPVSDWPGRLAAKFRNDFGDSL"
     gene            complement(22862..23389)
                     /locus_tag="PK288_03790"
     CDS             complement(22862..23389)
                     /locus_tag="PK288_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18074.1"
                     /translation="MIKIFGIKIIDRIKEAGLTQMVLSRHSGVITTRLGFHELSTELC
                     SREAYIILHLSAGEREIAALKADLNTIGGISLNEISFDDSRPYTGFSGEEGALRVIGI
                     LIENRDKDAIMSVQKTLTAYGCVIRTRLGVNEEFLGRPAGLVLLELTGDEIQMDLLEK
                     DLLKLPGIILGKMVL"
     gene            complement(23525..24907)
                     /gene="hydG"
                     /locus_tag="PK288_03795"
     CDS             complement(23525..24907)
                     /gene="hydG"
                     /locus_tag="PK288_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPM18075.1"
                     /translation="MKPFIDPDEIWNLINNVRPDKTRVREVIAKSLDKHRLTLEETAV
                     LVNSVGTDLFDEIREGARTLKEKVYGNRIVLFAPLYVGNKCTNNCQYCGFRVSNKDAV
                     RKTLTDSEIIKEVEALEDNGQKRLILVYGEHPEYSAEFIAHTVRLVYNVKKGHGEIRR
                     VNINAAPFDIEGFRKVGEAGIGTYQIFQETYHPEAYKWYHPGGRKKDYEWRLTALDRA
                     QEAGIDDVGIGALFGLYDWRFEVLSLVRHTNHLEACYNVGPHTISFPRIKDALSLNIS
                     NKYETSDEEFKKLVPILRLAVPYTGMILTAREAPDVRREVMQYGVSQIDGGTKLELGS
                     YSESKNEEQNLNREQFRINDPRSLADVIDELLDHGYLPSFCTSCYRMGRTGEHFMEFS
                     VPGFIKRFCTPNAILTLSEYLCDYATEDVATKGWDVIEKNLQTLEKGVAASTRDKIEK
                     IKKGERDLYF"
     gene            complement(25013..25936)
                     /locus_tag="PK288_03800"
     CDS             complement(25013..25936)
                     /locus_tag="PK288_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase"
                     /protein_id="HPM18076.1"
                     /translation="MRIFKSFEEAAGIPNPVVTTGSFDGVHIGHKTIINRLKMLAEKY
                     HGESVLITFYPHPRKVLYPDTTGKDLRLISSQEEKLELLRKAGLDNLIIKEFTRDFSR
                     ITSEQFVRDYLRGILGAKVIVAGFNHHFGFNQEGDYKQLWGMREKYGFEAEEIPEQEV
                     EHEAVSSTKIRQAIKEGYIQKANAYLDHYYIVMGRPVEFHPDNPALPVQMYRIPLTEE
                     NKLLPAKGIYAVSFESDCGSSRGMVIIDGPGNEPSEVILYLFNDGLRPEGRSSTISFH
                     KKIHGAVDINDPKTSAPRIKAAMEEISELIY"
     gene            complement(25953..26588)
                     /locus_tag="PK288_03805"
     CDS             complement(25953..26588)
                     /locus_tag="PK288_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPM18077.1"
                     /translation="MTNMKTLPAKTVERLSEYRRTLMECLKENKNYIFSHNLAARLHI
                     TAVQVRRDLMLIGYTSVLRKGYDVKVLIETISRIIDSEQGMNVAIIGIGNLGRALAAY
                     FKGKRSKLNLIASFDNDPQKINKVISGVKCYPYHDLEKTIKELDIEVAILTVPADFAR
                     EIAEETVRYGIKGILNFTTVPLNVPSGVYLEEYDMITSIEKVAYFVKENKL"
     gene            complement(26585..27721)
                     /locus_tag="PK288_03810"
     CDS             complement(26585..27721)
                     /locus_tag="PK288_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM18078.1"
                     /translation="MAVLKVLVIDDEPGIRSGVTRILGNFHVNYPFMDEDYTFEVMEA
                     ATGEEGISMIEQQNPDILLLDNKLPGIQGVEVLEYIRNRKFDTVVAMITSYASLDVAL
                     KAHKFGATDFIPKPFTPQELKSSIENITKQQYLKRITHRMNQEGKKIRYQFLSVLSHE
                     LKAPLNAVEGYLRMMQEKQMGENIRDYDNVVERSLQRIQGMRNLILDLLDFTKIRLER
                     KDEKIEAVNLADIASGAIVTVQPYAIQMEVNIKLDVKSDAVIMADPTDMDIIFNNLVS
                     NSVKYNKPGGEAEIVINGDESNVVVYFSDTGIGITESDRENLFTEFVRIRNERTRNIS
                     GSGLGLSIVKKVVELYNGTISVESTPDKGTTFIVRLPKKPQNNS"
BASE COUNT         7548 a         6661 c         6006 g         7642 t
ORIGIN      
        1 cattgtgcaa aagtcagatc gggtcctgat gaaataatct gacttttgca caatgagata
       61 ttctttctta tacccctcag gtttttcaat acctcaacaa agcccatgta agctgtattt
      121 ttgtaatgca ttaagagctt tgcacctgaa gcctccataa gatctcctcc ccagcattct
      181 acttcagctg cagggtcggc tttaataagt tcccgtacaa gattggcccc gtgaagatct
      241 cctgatttct cccctgctat aataaaatat ctcataaaag gaattttacc gtaaagacta
      301 ggactgccca ggcaattgtc ataccaagaa cgccccttga agcctgaagc atgtcgagac
      361 ggttgaatac aaggaacaat accagattgc aggtaacact cagcgacata atatgggtaa
      421 gaatatcggc ccttataatt ttcctgaaat aggatgggat gctcaggtcg ccgggggaga
      481 aaaaccagaa taggaatgcc gcaaccagtg gcagtataaa acctgctaag gcaccaacag
      541 gagttttgtc atatttcccg gtcccggtca tttgccgaat ttttccttca atgtgtcaat
      601 ataaccatat gcagtcatat caactgaaac aggaacaacc gaaacatatc catttgaaag
      661 gttccactcg tcggtatccg ttgcatccgg ctcatgatta ttgaacatac cggtgagcca
      721 gtaataggtt ttccccatgg gatcccttct tttttcaaat tcttctttcc agtttcccct
      781 ggcctgacgg cataccctga tgcccctgat ctggtcctcc ggtaatgcgg ggatattaac
      841 attaaggcat atcccgtccg gaagggattc atttaccaga tgcatcataa tgatccttat
      901 aaattttttg gatggtgaga agtccgctgt ttttgaaaag cttgaaagtg aaaaaccgac
      961 cgaaggaatg ccgtaaaggc atccttccag ggccgcagcc attgtacctg aataaagcac
     1021 gcttacagag gcatttgaac catgattgat ccccgacact acccagtcgg gcttcttgtc
     1081 agcaagctgg tttacagcca gtttaatact gtcggtcggg gtgccgttgc aggcatatac
     1141 tttcctggaa ccggtttctt ccagaagctt cacccttaaa ggtgttttta cggtcagggc
     1201 ctgcgacatt cccgacatgc cctcaacagt agaaataagt atcacttttc caaactcctc
     1261 cattacttcg agaagcgccc tcagtccggg tgcgtacaat ccgtcgtcat ttgtaagcaa
     1321 gatcagtctg tcgccttttt ccaccaccat gatttaatat tatataggtt acaaatatag
     1381 gcaaatacca ttctggtcgt gaaaaaaata ttaacctgca ggttgcatcc tgttcataat
     1441 gacgatgcca aagtttgatg aattttgaag ttactttttc caaaatgctt acttttgcat
     1501 tcctgatatt ttattcaaac tcctgatgaa gatttcttac aactggttaa aagattacct
     1561 cgcaacagac ctggccccgc aaaaggttgc tgaaattctg acagatattg gccttgagat
     1621 tgaaagcatt gaagagtggg aatccgtcag aggaggaatg aagaagattg tggtgggaga
     1681 agttctgacc tgtaccaggc atcccggagc cgacaggctt ttccttacaa cggttgatgt
     1741 gggatcgggt gaaccgctcc gcatcgtatg cggagcacca aatgttgcaa caggacagaa
     1801 agttgcagtt gcaaccgaag gggctgttat atatcaggat gaaaaggtca ttgaaataaa
     1861 aaaatcgaaa ataagaggtg agatctctga aggaatgatc tgtgcagaag atgaacttgg
     1921 cctgggaaat gatcataccg gaatcgtggt gctggacatc aatacaaagc ctgggactcc
     1981 tgcagcggag tatttcaatg tgatcagtga tacggttttt gtgatcggac tcactccaaa
     2041 caggattgac agcggttccc attacggtgt ggcacgcgat ctggctgcat acctcagcct
     2101 caatacaggc aagaccttca aagccaggct gccttccgtt gatgacttca aacccgataa
     2161 caccaacaat acctatgaca tcatagttga aaatccgatt gactgcccaa ggtatacggg
     2221 aatcacaata agcgacatca gggtaggaga atcgcccgaa tggctgaaaa accgcctgaa
     2281 agccatcggc cttaatccca taaacaacat agtcgacatc accaatttcg tacagaacga
     2341 aatcggacaa tcactgcatg ctttcgatgc tgaccagatc aggggaaaaa aggtcataat
     2401 aaaaaatctt cccgacaaaa cgaaattcgt tactctcgac gaaactgaac ggagcctgtc
     2461 atcacgcgat cttatgatat gcgatatcgc ggaaggaatg tgcattgcag gcgtattcgg
     2521 gggtatcaag tcgggaatta catccgaatc aaggaatgta tttcttgaaa gtgcctattt
     2581 caacccggtt acaatcagaa aaacctcgaa aagacacggg ttgcaaaccg atgcatcatt
     2641 caggtttgaa aggggcgccg acccgaatat tactgactgg gccctcaaaa gagctgctat
     2701 gcttatcagg gacatcgcag gaggaaatat ctcttcggaa attgtggata tctatcccaa
     2761 taaaatacag aatgtactca ttgaagtaaa ttacagcaat atcaacaggc ttatcgggaa
     2821 aaaaattgaa aagagcactg taaaaagaat acttgaactc ctcgagatta ttgtcaggaa
     2881 cgaggaaaat gactccctgc tgctcgagat accagcatat aaggttgatg taaagtgcga
     2941 agccgatgtg gtggaagaac ttctgcgtat ttatggatat aataatgtcc aattcagctc
     3001 tcatgtcaat tcaacgctga cttatattga aaagcctgat aaggagaaag ttgtgaatac
     3061 aatttccgat ctcctgtcgg acaacggctt cacagaaata atgtgcaact ccctcaaccc
     3121 ggcatcctgg tacgagaata accctgactt taactccgac acccttgtaa ggctggccaa
     3181 tccgctaagt tccgatctga atgcaatgag gcaatcactc ctgttcggag gacttaattc
     3241 tgtcgtatgg aatatacacc ggcagaatcc ggatctgaag ctgtatgaat tcggcaactg
     3301 ttacttcagg aaagacaata ttcacgggtt tccggaagcg gatgattata ttgaaaaaac
     3361 ctcgcttgat ctgttcatta ccggcaattt tcgcagggaa aactggaacc agaagacaaa
     3421 cccttctgat ttcttccata taaaatctgc tacggaaatg atcctcatcc gactgggtat
     3481 aaaaccggaa atgcttactg ccggcgaaag cgataaaagc tatttttccg aatccgcatc
     3541 ctactttttc aataacatgc ttgttgcaga gaccggaatg atctcaagaa attatctcac
     3601 atatttcgat atcgctcagg atgtttacta cactcatttt gaatgggata tgattatgga
     3661 gcttatcagg aacagcagga tcgtcttcag ggagctgccc agatatccca ccgccaggcg
     3721 cgacctggct cttcttgttg ataaagaaat ccgtttcgcg caaataagag agctcgcatt
     3781 taagacggaa caacgcctgc ttaaagaggt tggattattt gatgtttatg aaaatgaaag
     3841 ccttggtcac aataaaaaat catatgcggt cagttttatt ttaagggacg acgatcagac
     3901 acttactgac agatctattg aaaaggttat gaataatctt ataagagtgt atgaaaaaga
     3961 tctgaacgca aagatcagat agtactgccc agactccaaa ccaacaggac ctgctttatt
     4021 atggagaaca ttattaaacg gatcgccacg tatttgtccc ttgtaaaatt cagtcatact
     4081 gtttttgcaa tgccatttgc acttatcggc tttgccctcg cggtgagcga taggaatcat
     4141 gttttcagtg tgaagctcct tgtcctgatc atcctttgca tgatttttgc caggagcgca
     4201 gcaatgggat ttaacaggct tgtcgacaga aagtatgatt cccttaatcc gcgcacactt
     4261 ggccgggaaa ttcctgcggg aaaagtgagc ccttcatctg caaccgtatt tgttgttgca
     4321 aacagtcttc ttttcataat aacagccggc tttattaaca ggctcactct tatcctggca
     4381 ccactggccc tgacaataat cctgggatac agcctcacca aaaggatcac ctggttgtgt
     4441 catttcatac ttggccttgg attgagtctt gcaccgatcg gggcatatat ttctgtgacc
     4501 ggccagttca gcctcgtgcc ccttatatat tcactcatcg taattacctg ggtgagcggt
     4561 ttcgacataa tttatgcatt gcctgatgag gagttcgaca gggagaaccg cctgttctca
     4621 ataccggccg cggccggtaa aaagaaggct cttgtaatct cctcccttat acattttgca
     4681 actttcatcc tggtgatatt cgcaggcctg aaaagcatgg gaggtatttt ttactggatc
     4741 ggtgcagcaa cctttacggc cctgcttgcc tatcagcata tgattgtcag acctgatgat
     4801 ctttcaaggg taacacatgc atttcagaca accaacggct atgcaagcat tctttttgcc
     4861 acattcacaa ttctcgatct gatcatcagg catcctgcat aaccatgcta aattgatgat
     4921 attaatttca catgctggtc aatatggatc atattatcgt cagggaggtc cttagcagaa
     4981 gtgagctgaa aaagttcatt tcacttcccc ataaaataca caagacagaa aaaaactggc
     5041 ttcccaccat gtacactgac gaccggaagt tgttcagcaa aagcagaaac agatatttca
     5101 ggcactatca ttccatcttg ctgctggcct acagggatcg tattccggcc ggaagggtgg
     5161 ttgcccttgt gagaaaggat cctgatgcgg aaaccgacag aagctgccgc ttctgctata
     5221 tggatgccta tgatgatccc caggtggttc acgcactagt tgagagaatt gaaatctggg
     5281 caagggaaaa aggcgctgac cgtattatcg gacccctggg attctccata aaggatcccc
     5341 acggattcca ggtggaaggt tttggttatc ctccgatcat cgctactgcc aataactctc
     5401 catggatgcc ccgcctgatt gagaatgaag gttttgaaaa agacatcgat ctggttaact
     5461 acgtaattga cataccggaa gaataccctc ccttttaccg gaaagccttt gagaggatcc
     5521 ttggaaacag gaagatcagg atcgtggagt tcagaaaccg gaaagacatt aaaccataca
     5581 tcattcaggg actcgagctg atgaacgaaa cctactcgga acattatggt tatatcagca
     5641 tgacggatga agataaacag gaatttgccc gtgaatacct gccagtcctt gaccctgaat
     5701 ttattaaaat agttgaatac gatggagaaa ttgtttcatt catcatcggc ctgcccgatt
     5761 tcaaccaggg tataattgct tccaaaggac gcttgttacc ccttggcttt attcggatat
     5821 tgagatcctt taaaagatcg aaaaaactga tatttgtact gggagggata aaaaaatcgt
     5881 ttcgcggcat gggattggac attctcatgg ctgtaagaat gtttgaatca ggattgaagc
     5941 ataaaatgga atttatcgac agccatctca ttgttgaatc aaatcagaaa atgcgggctg
     6001 aatttgaaat tttcaacggt aagctcataa agagattccg gatttacaaa aaaacgatca
     6061 gaacctgaat gcaagagaga gccccaggag ttccttgaac tggatcctgg ctgttttctt
     6121 ctgaaggcca tcgggcccca gtaccgggtc ctttcccctg gtaaacagca gcgtcctggt
     6181 gtcatcatca aagatcagat gagtgttcag cctgatatcc gtaaaccagt tcaggctggc
     6241 aaccacaatc atctcccaat ctacatcgat attctgcgga ttgtggatat aattggtaaa
     6301 cagctgcagc ctgtttttta ccgaaactgt tttcaaaggt ttaaactcat gcgaaaccag
     6361 gaggctggcg ccgggttcat gttttgatct ccggtccttt gccactccgt atttcgtctg
     6421 gtctatcatc gatgtatccg gaacgaatgt gcccttatac gacaatggtg agagattgat
     6481 tgatgtaagc ttgttgggct tatagtcgag acccagccct agcgtcaggg tggccgggtt
     6541 caggaatttt gaaaccggaa tcgaatcgtt tggatagtca taacctttcg ttatctgggt
     6601 tttgaaaagg gcagtggcac tgaaatcgaa tttcccgaat gccttgtgat tcaacttgga
     6661 atttgtttca aaaaggtcga tattggttct tactccgttg tgggttgatg caatcaatcc
     6721 atatttcagc ctgacaaagt tattggaaga taccagatag ggcttgttca ggtaatcagc
     6781 gtaccaggta acatcaaaag ctgtggaaat attgctttcc ccccccttga cccagtttga
     6841 gagcagaccc tgattcaatg caagaattgc ctctgtccga tacttccata actgagcttt
     6901 ggtcacaatc ttcctgatat cctgaagttt ggttttatcc tgctgttcaa cgttaaccct
     6961 ggccccggaa atattcccct gcatgggcgg ccgcctgaaa ataaccccct gttcaagata
     7021 caggcctatg gtgtttctcc cggggctgcc aatccatacc gttaccgaat cattcaggtc
     7081 atttttcagc cagtaacgca ccattaagtc cgacttcgaa ttcaaccata tgtttgtctt
     7141 cccgctgtac atcccggtaa tggaaacaat gcttgaatca cgggcttcaa ttgcatcaag
     7201 caagaccgat acggcagctt ttaatgagtc accctggaaa gggtagcgat aatagcggaa
     7261 ggggaaatcc ctgtgagatg atgtaacata atccagtgtg tcaaccacca ccatcagtgt
     7321 ggtatctttc atgacaaccg tttccgcctt ggcagcaagt cgcactgtat cagtctcttc
     7381 aggttgttca gcagatatgt ccgggcttac ttttacaggt accctgaacc ttaccggttc
     7441 ccatatgtaa aactcatcac gctttatcct gatagaatcg taaggatatt tcatgaggta
     7501 agtccttgca gagtcgaacg gaggatttga ggcttcaaaa attacctgac caatcgcaga
     7561 cctgaacgga tcccttatat ttttccacaa atgaggctga tagctttttt gcagaaacct
     7621 tagggcctga tcgtatgata aactgatctc ccttctgccc gtatcagaca ccatcagcaa
     7681 tcccctggca ttaagggaat ctattatcct gagatctgta ttgtcaacta tattcttaac
     7741 ctgcggtaaa gcggggggaa tacaggttac acaaatcaga acaaacaaag ggagtaattt
     7801 ttttcctctc atagtcatct gcttttttga attcactttt ctgataaaac agaatcaatt
     7861 gtttttctca atacccgatt aaatgataac gataaatctc tcgtcttgca tataccggaa
     7921 ccggaaaaag tatcaacatt ctgtaaccga tttgttaaaa acacgcaaaa tacttataat
     7981 ctatatgtta atcgaataac accaggtttt tttaacaaat tttatttgcc cgttaagatc
     8041 gtatgccggg attgcagtgt catcgttcct ctttgaatat ccatgataaa tgttttcctt
     8101 tggcggttat ttagctcaaa tgcacgatct gacaacaggt aaggagggaa agctcatttt
     8161 ccagtttgct gcaccgatgt tgctcggaaa catatttcag cagctcttca gtgttgtcga
     8221 cagtatagtt gtgggcaatt atatcgggaa aaccgccctg gcagcaatag gtgcgtcgtt
     8281 tccggtcatc tttatgatga tttcgatgat aatcggactt gtaatgggca ccactatcgt
     8341 aatatcccaa tactatggtg cgggagatct ggtaaaagtt aaaagggcaa ttgacacaat
     8401 gtacatctac agtttcatcg ctggaaccat tgccactgtt gcaggaatca ctcttgccga
     8461 gccggttctc aggctgcttg cccttccgga ggagatcatg ccccaggcaa agcaatatct
     8521 cagaatttat ttttgcggta tgctgatttt tttcggttat aacggtacaa gtgccgttct
     8581 gcgggggctg ggtgattcaa aaacccctct ctattttcta attattggca ccatagcaaa
     8641 cataatcctc gtcatcctgt tcgtgggtat tttcagatgg ggtgttgcag gagccgccta
     8701 ttcaacgctg ctggctaacg gactggcctt tcttctggca atcatatggc tgaacaaaac
     8761 ccataaactg atcagaatag ccataagggg actgcacttt gaccgggaag tattcaggca
     8821 aagcctcagg ataggccttc ccacgggact tcaacaaacc cttatcgcgt ttggtaacct
     8881 tgctgttctg ggaattgtca atacttttgg tacaaacgtg gtcgcagcct tttcagtggc
     8941 ttacaggatc gacagccttg ccataattcc agctatgtca tttgctgctg ctttctcaac
     9001 atttgtcggc cagaatatag gcgcaaacaa aaccgacagg ataaggaccg gactgatttc
     9061 agccattaaa atgtcaggat ccgtgaccat tgtaacaacc attctcataa ttgtctttgg
     9121 aaatctcctg atgaatcttt ttacaagcga ccaggaagta attaaaatcg gcaaccagta
     9181 cctgacgata gtcagcagtt tctatcttgt gttcgcattg atgttcatct accagggagc
     9241 catgaggggt gcaggggaaa ccatgatacc catgcttttt tctcttttct cgctttggct
     9301 catccggatc ccgcttgcgt atttcctttc ccggaaaaca ggcgctacag gtatctggtg
     9361 ggccactccc gtaggatgga ttttcggact ggcgttctca tatgcatatt ataagacagg
     9421 cagatggaaa aagaaggctg ttgttaaata cgatcagaat ttgtaacttt caaaactaac
     9481 cggatcatca gatgattaga tcaatgacag gcttcggcaa agccattgct gaaacacccc
     9541 agaaaaaaat aaccatagag ataaaatccc ttaattccag gcagcttgat ctgagcacca
     9601 ggttaccaat gatatacagg gaaaaggaac ctgaaataag gaacctggtc agccagaaac
     9661 tcgaccgggg gaaaatagaa ttctcaattt accttgatat ccttggagat gagagcatac
     9721 cgctgatcag taaatcaaca gtcggaaatt attacaaaca gctcagggag atatcgtccg
     9781 aactggatat aaacgccggt gaacagctcc tgccggtaat tatgagactc cctgaagtcc
     9841 tgcagaccga aagggcggaa ctctctgagg aagaatggga actggcaaaa aaacagatag
     9901 ttgaatcgct ggaaatggtt aactcataca ggatcgaaga aggcaagtcg atcgaaaagg
     9961 atatcagggg ctgcctcgca cggatcctgg aatcacttga gatgatcggg gattttgaac
    10021 ctgacagaat aaccagggtc agggaaaaac tgctttcaat tcttgaggga aacctgttaa
    10081 ctgaaaatat tgacagaaac cggttcgagc aggagcttat cttctacctg gagaaatttg
    10141 atatcaacga ggaaaaggta aggctgagga aacattgcga atattttgtt caaaccattg
    10201 acgaagatgc tcccaacggc aggaaactga atttcattgc ccaggaaatc ggacgggaaa
    10261 tcaatacaat aggatcgaag gcgaatgatg cctcaataca gaaattagtt gtaatgatga
    10321 aagatgagct tgagaagatt aaggaacata ccaataacat tctctagtta cccagtatca
    10381 gcctatgaaa gggaaggtcg ttattatttc agcgccctcc ggggccggca agacatcggt
    10441 ggtgaatcat ctccttggga tggatctcaa tctggagttc tcaatatctg caacaacaag
    10501 agatccaaga ggcagtgagc agaacgggaa ggattattat ttcatgccag agcacgaatt
    10561 caggttacgg ataaagaatg atgaatttct tgaatgggaa gaagtataca agggtcagtt
    10621 ttacggtaca ctgaaaagtg aaattgaacg catctggtca cacgggaagc atgttctttt
    10681 tgacgttgat gtcagcggag ggattaatct caagaacttc ttcggaacaa atgcaatatc
    10741 aatattcatc atgccgcctt cggttgcaga gcttgaaaaa aggctgcata taaggggtac
    10801 ggatgaaaaa gcaaaaatca gaatccgggt tgaaaaggcg cttgaagaaa tcaggcttgc
    10861 cgacaggttc gataatatcg tagttaatat gaatctgggt gtggcacagc aggaggtata
    10921 cgcgatagtg caaagtttcc tggacaatta gtcggttatg tcgaaaattg gtttgttttt
    10981 cggatcattc aatcctgttc atatcgggca tctggccatt gcaggctata tgaaggaatt
    11041 taccgatatc gaccaggttt ggctcgttat aagtcctcag aatccactca aaaaaaaagg
    11101 aacactcctg gccgatcatc accgtttgta tcttgccgaa cttgcaattg gggataatga
    11161 tgcactgaag ccatcagata tcgaattccg ccgtcaggga ccgtcatata caatcgatac
    11221 gatggcgctc ctgtctgaaa agtatccgcg gcacagattt gttctgataa tgggcgagga
    11281 caatctgtac acacttcata aatggaagaa tgcccgggaa ctggtaaagc gttatccaat
    11341 actggtgtat ccgcggcccg ggatttccag gaaggaaaac attcagctcg acgagatcct
    11401 gtccatggca accatcaggc gggtcaatgc accactgatg gatatctcgg gcagctttat
    11461 caggaatggc atccgcaggg gcaagaacat gtcatactac cttcatccgg cagtgtggca
    11521 gtatattgta tacatgcatt tctacgaaaa gtaatccgct gtgtaaaaat ccgtaaagac
    11581 gatgaatggg tgacatacga aatgacatgc tccccgatcc aggctgccat cacccgggtt
    11641 atttatcctg ttccctgatc cttagcactt cacgcatcat ctcaccacct ctctgcaatg
    11701 ctttggcatt ttccggtatt agcctgtgat gcctctccgg gagtgatttt ttcaggccat
    11761 tcacaacatt ctccacgctc agtacaggtt ttatttcaag aaatcctccc aggattatca
    11821 tgttaaatgt tttcgggctg ttcaatctgg cagcttcttc ggttgcatcg atcctgtata
    11881 tggaaatgtc atttcttacc ggatgcctgc tgatcccgtt cccgtcatac accaggacac
    11941 cccctggttt aattgaaccc tcgaacttat cgagcgactg ctggttgaga attatgcccg
    12001 tatcaaaacg tctgataatg ggtgaactta tacgttcagt gctcagaatg acgatcacat
    12061 tggccgttcc acccctcatc tcgggtccgt aagagggcat ccagcttacc tccatatcct
    12121 gcaaaacccc tgcatatgcc atgatcttac ccattgataa aacaccctga cccccgaatc
    12181 cggcaattat tatttcctct gtcattgctt taattttata ttcatttacc ggtcttttca
    12241 tcaggtactt tcatatcgcc caatggataa aaaggcagca tgttctcctc catccacctg
    12301 atcgattcca caggtgtcat tttccatcct gaagggcaat tggatacgat ctccacaaaa
    12361 catgtaccct tattatgaaa ctgatactgg atagccttgc gtaatgccct ttttgttttt
    12421 tttacattgg cgggagtgtg aacactctgc cgggtcacat aatacgtgcc tggtaatgtg
    12481 gcaacgatat ctgtcatctt caggggattc cccatcatcc tgacatctct tccgaagggc
    12541 gaggttgatg atttcattcc cgggatggta gtaggggcca tctgccctcc ggtcatcccg
    12601 taaataccat tgttaatgaa aataatgagg aaattttcac cgcggttgca tgcatggatt
    12661 gtttctgcag tgccgatggc ggcaagatcc ccgtctccct gataggtgaa tacaaacctg
    12721 tcgggcagga gcctcttgat ggctgttgct actgcaggag cccttccgtg ggcagcttcc
    12781 tgccagtcaa tatccagaaa attgtagagc agtacagaac atccgaccgg agacacccca
    12841 attgccttgg attgaagtcc ttcttcctca ataagttcgg caagaatacg atgtgcagtt
    12901 ccgtgcccgc atcccgggca gtatgaaaga accgtatcag tcataatccc ggttctcttg
    12961 taaaccagat tttcaggttt tattatttcc ttaatcccgg taacctcagc catctgctaa
    13021 cctcctataa tttttgatct aagattctca acaacttctt cgggagtagg tatcattcct
    13081 cccattcttc cgtaatgttt cacagtaact ttcccgttga cggcaagaag aacatcctca
    13141 accatctgac ccgcactcat ctccactgac agaatacctt taaccctgtc tgccagcacg
    13201 gaaattcttt ctgagggaaa cgggaaaagg gtaattggcc taagcaatcc aaccctgatc
    13261 ccctccattc ttgcaagctg gaccgatttc tggcaaaccc ttgcacttgt tccataagct
    13321 acaagcaaat aatcagcatc atcacactga aactcttcat atctcacctc tttctccctg
    13381 atctcattgt attttgcaac cagcttgaga ttgaagcgtt cctgtgcttc gggatcaagg
    13441 tcaagggaag tgattatgtt ccggctccgt gtagggggtt ttcccgtggt tgcccactca
    13501 ggaacatctt ttgatctctc cctgtgaggg ttcagatata ctttctccat catctgacct
    13561 atggccccgt cggagagtat cattacggga ttcctgtacc tgaaggcaag atcaaaccca
    13621 agttcgacaa aatcagccat ttcctggact gatgcaggtg caaggactat aagatggtaa
    13681 tccccgtgcc cgcctccctt aaccgcctgg aaataatctg actgtgcagg ctgtatggta
    13741 ccaagccccg ggcctcccct gacaacgttt acaataagac agggcaactc cgcaccggcg
    13801 atgtatgtta ttccctcctg ttttaggctt ataccgggcg atgaggatgt ggtcatcacc
    13861 tttttaccgc atgccgcgcc accgtaaacc atatttatcg cggccagttc actttccgcc
    13921 tgaagaacca ccatcccggt cgtctcatat ggttttgcct ccataagata ctcgaggatt
    13981 tcactttgcg gggttatagg atacccaaaa taaccgtctg cccccgccct tatggcagct
    14041 tccgcaaccg cctcattccc cttcatcaat cttaattccc gttccatagg ctggttaaat
    14101 ttttgtttta taaacagtga tgactccgtc aggacaaaca atcgcgcagt tggcgcaccc
    14161 tatgcacaaa tccggatttg ccggataagc aaaattatat cccttggagt tcacttcctc
    14221 tgccattgca atggtaccgg ttggacacga gatcatacat atttcacaac ccttgcacct
    14281 gtcaatattt acaacaatac ttcctctgac ctttgccata attaaacctg attttggttt
    14341 tacacctgat gatcagcacg gaaacgtttc aaacgcaatt ccaggtcgcc gagaagagac
    14401 atcggaacct ttgaaacaca ggccctcagg ccttcaagcc tttcgcttcc cgtggtatca
    14461 agtgttatcg cacttatccc gtaatgaagg agctcactga gcaactgctc tcccgacatg
    14521 ccgggataag atattgtaaa atagaatccg tcgcctatta actcgtccag atccttgtca
    14581 taaactatat taaacccatt ctcagtatac aaccgcttca taatttttgc tttttcctca
    14641 tattccgaaa gcatttcacc gaaccgcagg gttccgtcat tgactgcctt cagcaatgcg
    14701 gcaagaccat actgtaccga atgggcaaca cctgcagaga gtccgtaaag cgcaccgaat
    14761 attgcggaat ggccgaactt gtcagatgaa aaatacctga gcagatcggg ataggaccgg
    14821 ttgaacaggt ggtcggaaat cgccatgatg ccaattctct ggcctgcata gctgaaaatc
    14881 ttggagcttg agatcaggat cacgtaatca tcggtgtaac gggcaacagt gggctggtaa
    14941 ggcggttctc ccggatttga atagtttttc ctgaaatcca ttccgaaata agcaagatct
    15001 tcaatgacaa taacgtcgta tttacgtgaa agttcaccga taattgcaag ctcatcctca
    15061 ttcaggcata tccatgcagg attattcgga ttggaataca gaagcgatga taccttaccg
    15121 gtttccaggt aagattcgag ttttgccctc agcttttttc ccctgaaatt atacacatca
    15181 aaggaaaaat atccatgtcc tagcatcttg acctgttgct tctggaccgg aaagcccgga
    15241 tcgataaaaa gggttccttc ctttgtccgg tcatttcggt tggccacaag aaagcacagc
    15301 attgagccaa gcatagatcc gaccgtaggt acacagccca aaggatcaac atcaacattg
    15361 agaaaaagct ttatgaatcg tgatgcttcc tttttaagct ccggcaagcc tgttatatcg
    15421 gggtatattg cagctactcc ttctttcagt gccctgatct cggcttcaat ccccaccgac
    15481 ggtgccggga ggcccggaac tcccatctcc attcttatga attttatccc tgtttccgat
    15541 tcgataaggt tgaccagtgc cacaatctca cggatggagg cattgcccaa taacttgagc
    15601 cccatattgt ccatgattgg cctgattatg ttatctgaaa ttggaatatc attcatttta
    15661 acacctgaat ttctcttttg tcaattacac gttttgcttt gccttcactt ctctcaagag
    15721 ccttgggttc aaccagggtc accttaattc caaggcccag ataactctcg agggttccct
    15781 gtatttttga ccgcagcccc tccagctggc taatcctgtc aaggaaaaaa gcttcatcaa
    15841 cttcaacctt gagttcaagt gaatcgagat tattgatcct gtcaacaatt agctggtaat
    15901 gcggcgttat cccgcccatt tccagaagga tactctcaac ctgggacggg aacagattca
    15961 cacccctgat tatcagcata tcatcgcttc tgcctacgca tttgtccatt cttacgaagg
    16021 ttctgccgca tttgcaccta ccgtaattaa ggcgcgtgag atcgcgtgtc cggtacctga
    16081 ttaggggcag tccgtgcttg gtaatagtgg taaataccag ctcgcctgtg ctcccttcag
    16141 gtaatacctg caaagtttcc ggatcgatta tttcgggata aaaatgatcc tcattgatat
    16201 gaagtccttc ctggaaaagg cattcacttg caacgcccgg gccaataacc tcgcttaaac
    16261 cgtaaatatc tatcgccttt attctcagct tgtcttcaat ctcccgtctc atgttgtcgg
    16321 tccatggttc agctccgaaa accccgaccc tgagcctgaa ctcatcccta ctgatgccgc
    16381 tttcaattat agcttctgcc aggtaaaggg catatgaagg tgtgcaggca aggaccgtac
    16441 tgccaaaatc gtgcataagc tgaatctggc gcaatgtatt accgcccgaa atgggtatca
    16501 ccgaggcccc gatcttttcc cccccgtaat ggagccccag tcctcccgta aaaagcccgt
    16561 atccgtaggc aatctgaata aaatcattct tgtcagctcc cgcacatgta agggtccttg
    16621 ccatcacctc cgaccaggta gcaatgtcat tacgggtgta tcccacaaca gtgggcttgc
    16681 cggtagtccc tgaggatgca tgaatcctga ctatttcact cagaggaacc gcaaacagtc
    16741 catagggata gttatccctg agatcctgtt ttgtagtaaa cggaagcctg gtgatatcct
    16801 caaccgaacg gatgctctcc ggtccaattc ccaattcctg catcctgtgc ctgtaatagg
    16861 gcacattaaa atatgttctc tctactgttt caataagccg ctcaccctga acttttctca
    16921 gttcattgcg gtccatgcat tcaaaatgcc tgttccagat ttccatatca gatcttataa
    16981 aggtcgctcg cattaatcaa ctccatctca tgatccttca aagcctttat agcctgcccg
    17041 ttattattgc atcttaagat tattatcgat ttggatccgc atgaaaatgc gtatgtgtat
    17101 tcaatactta tttcctgatc agacaggatc ttgagaatct tagcataata tttggcctca
    17161 tcaggggcca taactgaaat aacttcagac aggttaaccg tgaaatgatt atccttcaac
    17221 aggcttttag ccttctcagt atcagataca atcagtcgca ggatcccgaa ttcggaagta
    17281 tcagcgacgc taagcgctgt tattctgaga gcttcctttc ccaggacttc caggacctcg
    17341 gtaagtcttc ctgacttgtt ctcgaggaat actgacagtt gtggtataag catttgggct
    17401 ggtattaatc attgaaaaat caagttccga acttacatat tatttttatt tgttcgacta
    17461 aagcgcacca atatcatttt tcaatcctta cccttgcatc tacaacggta attccttcag
    17521 ggcttccaat cagcggattg aggtccatct ccttgatttc cgtggcaaag cgcaggagac
    17581 ttgaaagcct tacaatgatc tctgcaaatt tcagttcgtt tatccccggt ttgccgcgtg
    17641 tgcctttaat gattttgtag gccctgagac tttttatcat tgaaccggct tcgttgaatg
    17701 taagcggtgc gaggcccgag gatacatcgc ccagaacctc cacaaaaatc cctcccaggc
    17761 cgcaaagtat tatgtgcccg aaccgcggct catattttgc tccgataaag agttccgtcc
    17821 ctgaaagcat cttctggatc attaccccgg ttacgccagt tatctttgtc atgcggctga
    17881 actcggcttc aagatgcctc tgcgatttaa tattcagggt aactccgcca acatccgatt
    17941 tatgaacagg tcccacaacc ttaagaacaa gcggataacc aaactcctct gccagtatga
    18001 ccagatcctt acgcgatctt gcaattgcct ccttcactac aggtatccgt gcagccttaa
    18061 gcagctgctg gataagctcc ggttcaagat atccgctgac tgaattatca actatttccc
    18121 tgatcttccg aacatcaata ttctccagga agatcttctc ttccgccggg aatggggtat
    18181 tcgagatcct ggtcagggcc cggcccaata caacctcatc gggaaaattg acgtgcccct
    18241 tgccaataaa atcctcaagc tctttcgatg agcttgaaac agaaggcagg atcggataca
    18301 gaggcttgct gcattcgttg atcttcctgt gaagcagatc ataaatctca gtcatgtcgg
    18361 taagaccgtt attgccgaaa ataacactca tcccgtctat catttccagc tgcctgtcgg
    18421 cataatcaat gatcatccca agctgatcaa tagtagctgt agctatcata tcaatcggat
    18481 ttaccgctga agctccggga ttcatcatgg cgagtatcct gttctgatgt tctatatcaa
    18541 gttttggaat attcattcct ccggaagaaa gtgcatcggt cagcataaca gccggcccgc
    18601 ctgcatgtgt aacaatcgca atattccgtc ccctcaatgg cttgtgcata aaaacggagg
    18661 ccacggttgt cagctcttcc cgtcctgaac accgtactat tccggctttc ctgaaaagag
    18721 cctcaacggc cgagtcggaa gaagcaattg ctccggtatg ggacgatgca gccctgcttc
    18781 cggcctcggt ggtcccggct tttattgcgg caatacggca tcctttctga atcagtgagg
    18841 atgcatgccg gagaagcttg tcaggatccg gaattgtttc cacataaata agttttatcg
    18901 gggaacttct ggcaggatca tatgtaagat cccagtattc cagcacctcc tcaacacctg
    18961 tctgggcgct gttgcccacg gaaaaaacac tggaaaacct caaacctttc ggaagtgccg
    19021 attcaataat aaataatgca gttgctcccg aaccactgac aaaatcacac cctctttccg
    19081 agagtctggg aaccggagtg gtaaatacac cctgatacga acgggtgatg acacctatgc
    19141 agttcgggcc aatcaggcag ccgccggcct catcaatcag tccggcaata tcttcttcca
    19201 tcttcctgcc ttcttcaccc gcctcgctga atccggcgga aataattata aaagcctttg
    19261 tattcttttt cctgacaaga acatccacta cctggtaaca gtacttggct ggtatcccca
    19321 aaatcgcgag atcgacaccc ggaatatcat tgatgtcccg gtaagatctc cttccctgaa
    19381 tgaaatcatg ccctggattc accacaaaca gctcaccggt ataacctccg tcaatgatgt
    19441 ttttcaggat tttgccgccg ggtttcataa gatcgtcaga acctccgaca acaacgatgc
    19501 tggcaggact tataagtttc tcatttaaca tagtcaccct ccagtctgag taatgatccg
    19561 gttttgcatc aaagtcaatg tcagtaagta tatatcacag accaccaggc agtttgagag
    19621 agttcacgtt atttgcccaa agggtaataa ttgtttgtgc catttgggaa attacaaaca
    19681 ataccccggt tattccaata atagcaatat actaaaaaaa aacttcacaa aacctgaaag
    19741 taatcatgtt ggcaggatgt tgcctactga cgattttaaa agctattttt gcacgtgaaa
    19801 accattgccg ggatccgttg aaatgatgac agacacccgg tgaaggacaa tcgggagatt
    19861 gttcaaagat tttcatttca gtgaacaata attttacaag tgagtttaac attgaattgc
    19921 tcaggcgata attaataaca gaaaaatggg aatagacaga ataattgaac aggatgcctt
    19981 tgccaggaat gatattatcc gcctgttgca gtccgaaggg ccggacaaag aattactttt
    20041 cagaaaatca gcggaggtaa aggaaaagct gataggtaag aaggtctggc tgagaggact
    20101 gatagagttt tcaaatatct gcagcaagga ttgtctatat tgcggcatca gaagaaccaa
    20161 taaaaacctg ttgcggtaca acctgccgga cgaggaaatt ctttctgccg cgaagtttgc
    20221 ctatgataac cgttacggat ccgttgccct gcaatcgggg gagatggaaa gccgttcagt
    20281 taccctccgt atcgaaaatc tggtaaggaa gatcagggag ctgtcggggg gaacgctcgg
    20341 gatcaccctt tcggttgggg aacaggaacc tgaagtatat aaaagatggt ttgatgcagg
    20401 tgcccaccga tacctgttgc gcatcgaagc cacaaacgaa tcgctgtaca ggaaaataca
    20461 tccccataat ggcagatatg atttcagaag aaggctggat tgcctgaagg ctcttcagga
    20521 cattggttac cagaccggta ccggtgtcat gatcggactg ccattccaga caatggatga
    20581 ccttgcaggg gatctgttat tcatgaatga attcaatatc gacatgtgcg gaatgggtcc
    20641 gtacatcgaa catgccgaca caccactggc aaaacactct gcagatctgc ctccgctttc
    20701 agcacgattt gatctcaccc tgaaaatgat cgcaatcctg aggttaatga tgaaagatat
    20761 aaatattgtt gcggcaaccg ctctccaggc tattgatccg gtcggaaggg aaaaggccgt
    20821 aaggatagga gcaaatataa ttatgcccaa tatcacacct ggcccctatc gtgacagtta
    20881 caggctttac gacaacaaac catgcacttc cgactctgcg ggggattgcc gtgattgcct
    20941 ggaggcaagg cttagccttg ccgatcatga aattgtttat ggcgaatggg gcgattcaat
    21001 acattatgaa aaaagaagga aagggaaaca acaaaacccg gacaaatgac cgcatatcat
    21061 tatttcccca tccatcagga tcttttaaca accgcattgc accggtaaca ttgtttcagt
    21121 cattatcact cgaattattt caaaagataa agcgatctcg tataaaaatc cctgaaatgc
    21181 ttttgttttt tgaataaatt taatctaagt ttgccgcaca cttgaaaata tgaagttcag
    21241 gaacaatctg cacaggtact attactatta cttcatgatt ccgatggtaa gtcagaacat
    21301 ggaatagtgc cggtgacaca atatctgaaa aggcttacca agacaaggta agccttttta
    21361 tttctaaaat ataaaaacaa gagattatgg agcaaacaat tgcaggcagc gaagtaaaat
    21421 tcttcaatgg caaacaaatc cccgttgaaa tgcataaggt caggatcgtt cagtcgctga
    21481 accttgtgcc ggtagagcgc cggcttgagg cgatagaaga agccggttac aattcatttc
    21541 ttctatcaac atccgatatt ttcctcgaca tgcttaccga cagcggaaca aacgcgatga
    21601 gcgatgcaca ggtatcaaaa atgttcgtgg ctgatgacgc ctatgccgga tcgcagagtt
    21661 ttgaaaagtt tcagaaaagt gtattaaagg tttttggcaa gaaatatgtg ctgcccgttc
    21721 accagggccg tgcagccgag aatatcctgg gcaagtcata tgtaaaggaa gggtcggtgg
    21781 tacctatgaa ttatcatttt acaacaacac ttgcacatat tcaacttaac gggggcaagg
    21841 tccttgagct ttatacggct gaagccatga aaatcaaaag caccaatccg ttcaagggga
    21901 atatcgacat taaccggctt gaggaggcaa tacgcactta cggcagggag aatatcccgt
    21961 ttatcaggat ggaagcttcc accaacctca tcgggggaca gcctttttca ttggcaaacc
    22021 tgcgcgaggt aagaaaagtt gccgataaat acgggcttaa ggttgttctt gatgccagcc
    22081 tgatcgggga aaacgcctac ttcataaagg tcagggagaa agagtacaaa aactgcactc
    22141 ttgaatcaat attgctcgag atgagcggat tgagcgatat tgtatacttt tccggtcgca
    22201 aggttagttc ctcaagggga ggagccattt gcacaaacga cctggagatt tacaaccagc
    22261 tgaaatatct agtcccggtt tatgagggat ttcttacata tggcgggatc cccatccgtg
    22321 agattgaagc gatggccgaa ggtttgaatg aaactaccga agtttcggta gtaaaccagt
    22381 ctccgatgtt catcaattat atggtccgcg aacttgacca gctgggtatc ccggtaatta
    22441 tgcctgccgg agcgcttggc tgtcatgttg atgcaatgca gttcctgagc catgttccgc
    22501 agcctgaata tccggccggt tcgctggtgg cagcatatta cattatttcc ggaataaggg
    22561 gaatggaacg aggaaccatg agcaataaca gggacgaaaa cggtgtggat attcttgccg
    22621 atctggaact tatgcgactg gccgtcccaa ggcgggtatt taccctgtcg cagattgaat
    22681 atgcaatcga caggctgaac tggttatatc agaacaggca tctcgtggga ggactcagat
    22741 tcaccgagga accacccata ctcagattct ttaccggcag actcgtgcct gtctccgact
    22801 ggcccggcag gcttgctgca aaattcagga atgattttgg cgacagcctg tagcaggaac
    22861 attacaacac catctttccc aggattattc ccggcagttt aaggagatct ttttccagca
    22921 ggtccatctg tatctcgtcg ccggtcagct cgagaaggac cagccctgcc ggccggccaa
    22981 ggaattcttc attcacaccc agccgcgtgc ggatcacaca cccgtacgcg gtaagtgttt
    23041 tctgaaccga catgattgca tccttatccc tgttttcaat aaggatacca attaccctca
    23101 gggctccttc ctcaccggag aagcccgtgt aaggcctgct atcgtcaaat gatatctcat
    23161 tcagggagat tcctccgata gtattaagat cagccttcaa tgcagctatt tcccgttcac
    23221 ctgcagacag atgaaggatt atataagctt cacggctgca cagttccgtc gacaattcat
    23281 ggaaacccag acgggtggta atcacacccg agtgccgtga aagcaccatt tgggtaagtc
    23341 cggcttcttt gatccggtcg attatcttaa tgccgaaaat cttaatcatg tcagaaaggt
    23401 ttattgttat ttatttaaca ggtaatcagt gatatatccg gccgcaccgg gtcctttatc
    23461 ctttccggag atattttcca aaccaataac tccctggaga aacaggcatt attctatgaa
    23521 gatcctaaaa atacaggtcc ctttcgccct tctttatttt ttcaattttg tccctggtgc
    23581 tggcagcaac gcccttttca agggtctgaa gattcttctc aataacatcc catcctttgg
    23641 tggctacatc ctccgtggca taatcgcaaa gatattcgga cagtgtcagg attgcattgg
    23701 gggtacaaaa ccgtttaatg aagccgggga ccgaaaactc cataaaatgc tcgccagttc
    23761 gtcccatccg gtagcatgaa gtacagaagg atggcaggta accatggtcg agcagctcat
    23821 caatcacgtc tgccagcgac cggggatcat tgatcctgaa ctgctccctg ttcaggttct
    23881 gctcttcatt ctttgattcg gagtaggatc ccagttcaag ttttgtacct ccgtcaatct
    23941 gcgacacacc atactgcatt acttcccttc taacatccgg tgcctccctg gcagtaagga
    24001 tcattcccgt gtagggaact gcaagcctga ggatgggcac cagcttcttg aattcctcat
    24061 cgctggtttc atatttgttg ctgatattca ggcttagtgc atcctttatc cggggaaagg
    24121 agattgtgtg cggaccaaca ttgtaacatg cctcaagatg atttgtatgg cggacaaggc
    24181 tcagaacttc gaatcgccag tcgtaaagcc cgaacagtgc tccaatcccg acatcgtcaa
    24241 tacctgcctc ctgcgccctg tcgagcgccg taagcctcca ttcgtaatcc ttcttcctcc
    24301 cgccgggatg ataccatttg tacgcctcgg gatgataggt ctcctgaaag atctgatagg
    24361 tacctatccc tgcctcgcct actttcctga aaccttcaat atcaaatggc gcggcattga
    24421 tgtttaccct ccttatctca ccatgaccct tcttgacatt atacactaac ctgaccgtgt
    24481 gagctatgaa ctcggcagaa tattccggat gttctccgta aacaaggatc agccttttct
    24541 ggccgttatc ctcaagggcc tcgacctctt ttattatttc actgtctgtc agcgtcttcc
    24601 ttacagcatc cttgtttgat accctgaaac cgcaatactg gcagttattg gtgcatttat
    24661 ttccgacata aagaggggcg aacaacacta tcctgttccc gtaaaccttt tccttgagag
    24721 tccgcgcacc ctccctgatc tcgtcaaata aatccgttcc tactgaattg acaagcaccg
    24781 cggtttcttc aagcgtcagg cggtgtttgt caagtgactt ggcaattact tcccttaccc
    24841 tggttttatc gggccgcaca ttgttgatca ggttccagat ctcatccgga tcgatgaatg
    24901 gtttcatcct ctgatcaggt atacttaatt tctctggtga aaaacgcatt tctcttcttt
    24961 aattttatcc cgcaaaatta ctcattcaaa acaaggaaga caaatgatgg gatcagtaaa
    25021 taagctcgct gatctcttcc atggctgcct ttattcttgg tgctgaagtc ttggggtcgt
    25081 taatatcaac agccccgtga atctttttgt gaaagcttat ggtagagctc ctgccttcag
    25141 gacgaagtcc gtcattgaaa agatatagaa tcacttctga cggttcattc ccggggccgt
    25201 caattatcac cattcccctc gaagaaccgc agtccgactc aaatgaaacg gcataaattc
    25261 ctttcgccgg cagcagcttg ttttcctcgg taagggggat cctgtacatt tgaaccggca
    25321 gggccgggtt gtctggatga aattctaccg gccggcccat tacaatgtag taatggtcga
    25381 gatatgcatt ggccttctga atgtagcctt cctttattgc ctgcctgatc ttggtagaac
    25441 tgacggcttc atgctccacc tcctgttccg ggatttcctc ggcttcaaat ccatattttt
    25501 ctctcattcc ccaaagctgt ttataatccc cctcctgatt aaatccgaaa tgatgattga
    25561 atccggccac aattactttt gctcccagga tgcccctgag ataatcacgg acaaactgct
    25621 cgctcgttat ccttgaaaaa tccctggtaa attccttgat tatcaggttg tcgagacctg
    25681 ccttccttaa aagttcaagc ttttcctcct gcgaactgat cagcctgaga tcttttccag
    25741 tcgtgtcggg atacagcact ttcctcggat gaggataaaa agtaataagc acagattccc
    25801 cgtgatactt ttctgcaagc attttcagcc ggttaattat cgtcttatgc ccgatatgga
    25861 ctccgtcaaa tgatccggtt gtaacgaccg gattcggtat gcccgccgcc tcttcgaaac
    25921 tcttaaaaat tctcatcaac cggccggcac gattaaagtt tgttttcctt tacaaaatat
    25981 gccacttttt caattgaggt aatcatatca tattcttcaa gatagactcc cgacggcaca
    26041 ttcaggggta cagttgtaaa attaaggatg ccttttattc catatcttac cgtttcttcg
    26101 gcaatctccc tggcaaaatc ggcaggtacc gtaagaattg caacttcaat gtcaagttct
    26161 tttatggtct tttccaggtc gtggtaggga tagcatttta cgcccgagat taccttgttg
    26221 atcttctggg gatcattgtc gaatgaagcg ataaggttta gtttcgatcg cttacccttg
    26281 aaataggctg caagagccct cccaagatta ccaatcccta taattgccac attcataccc
    26341 tgttcggaat caattatcct gctgattgtt tctatcagga ccttaacgtc gtatcctttt
    26401 ctcaaaacac tcgtatagcc tatcagcatc agatcgcgcc ttacctgaac ggctgtaatg
    26461 tgaagcctgg cagcaagatt atgggaaaag atgtaattct tgttttcctt aaggcattcc
    26521 atgagggttc ttctgtattc actcaacctc tcaactgttt ttgcgggtaa tgttttcata
    26581 tttgtcatga attattctgt ggtttttttg gcagcctgac aatgaaggtg gtccccttgt
    26641 ccggcgtgct ttctacagag attgtaccat tataaagttc tactaccttt ttgactattg
    26701 aaagcccgag tccgctcccg gaaatattcc tggttctttc gttcctgatc cttacgaatt
    26761 cggtaaacag gttttccctg tccgactccg tgattcctat gccggtatcg gagaaataca
    26821 caaccacatt gctttcatcc ccgttaatta caatttcagc ctctccgccc ggcttattgt
    26881 atttaactga atttgagacc aggttgttga atataatgtc catatcggtc ggatcggcca
    26941 tgatcaccgc atcggatttc acatcgagct taatgttaac ttccatctgt atggcatatg
    27001 gctgaacggt aacgattgcg ccggaagcga tgtcggcaag attgaccgct tctatctttt
    27061 cgtctttcct ttcgagcctg atctttgtaa aatcgagaag atccaatatc aggttcctca
    27121 ttccctgtat tctctgaagc gaacgttcaa caacgttgtc atagtcgcgg atgttctcac
    27181 ccatctgctt ttcctgcatc atcctcagat agccttcaac cgcattgagg ggggccttca
    27241 gttcatgcga caaaaccgaa aggaactggt accttatctt tttgccctcc tgattcatcc
    27301 ggtgggtgat ccttttaaga tactgctgct tggtgatgtt ttcaatgctt gatttcagtt
    27361 cctggggtgt aaacggcttg ggaataaaat cagttgcccc gaacttatgg gcctttagtg
    27421 ccacgtcaag agatgcgtat gaggtgatca tcgccactac cgtgtcaaat ttcctgttcc
    27481 gtatatattc cagtacctca actccctgga tgcccggcag tttattgtca aggagaagta
    27541 tgtcgggatt ctgctgttca atcatactga tcccttcctc gccggttgcc gcttccatca
    27601 cttcaaatgt ataatcctca tccatgaacg ggtaattcac atggaagtta cccagtatcc
    27661 tggtaacacc cgaacggatc ccgggctcat catcgatgac cagtaccttt aagacagcca
    27721 tatcatattt ttaacagtcg cattatctcc tgatcgagct gatcggaacg tattcctttt
    27781 tcaagatacc tgtcggccct tatccacatc ctgtcgtgtt ccgagtcaag gctgaaggaa
    27841 atgcccgtct catgggc
//
LOCUS       DAOOYG010000024        27237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1548_length_27237_cov_8.269480, whole genome shotgun sequence.
ACCESSION   DAOOYG010000024 DAOOYG010000000
VERSION     DAOOYG010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1548_length_27237_cov_8.269480"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1266
                     /locus_tag="PK288_03815"
     CDS             310..1266
                     /locus_tag="PK288_03815"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF000824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HPM18079.1"
                     /translation="MERQIHKRKVVITGAGSVGATYAYALAQSGVAEEIVLLDRNEEL
                     LKGQVLDLVHGQPFFPTVSIRAGNDDDYGDASLVVITAGSAQKPGETRLQLLQKNAGI
                     VGSISETVARSGCRGVMLIVTNPVDVMTYVAFRYSRWSRNRVIGSGTVLDSARLRHFI
                     SLHCGVDPHSVHGYVLGEHGDSEFAAWSMIHIGGIPIDKYCSHCGKCEGWDEQKKLWE
                     QQVRDSAYHIIDAKGSTYYAVGLAMVRITSSILRNQNSMLTVSVIPDGEYGLKNLAIS
                     IPSIVSSAGVAGITSVPLPQDELEKLYNSASVIRKAIEETGI"
     gene            complement(1282..2814)
                     /locus_tag="PK288_03820"
     CDS             complement(1282..2814)
                     /locus_tag="PK288_03820"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPM18080.1"
                     /translation="MEKNKASKKGSQSGASAGSRPNMISRRKVLKGLILVPVQWSLFL
                     SAVRAQGNVRDLPWENNLKGGTDQWLPDLLPEDLYIPNRPVGTGQGIMPGRVSWVWNP
                     SCTNPACANKPVTGANPAEKYDAWFMDNNTDQKILDKMLLAGLCSMTGKENVNDAWDA
                     VFRFHNRKRGKGSVPYSKGEKIYIKLNRTTASGEIGPDYARPKEKLLPLACETSPHLV
                     ISMLRQLVNVAGVPQESIYVGDAMRNIYQDEYIKYHSEFPRVNYLSSMGATHGRITCG
                     ESKKDLIFYSDRKSVMGNAGSDKIYTVLEEAEYLINLAAMKGHGLAGITLCAKNHFGT
                     QSRRSAAHLHPGLNTGGNRKGYGHYRVLVDLMGNKYTGDKNLFYILDALWSGTDWNGL
                     PVKFLMPPFNNHWSSSLLLSLDPVAIESVAYDFLRTEFSYPEHTVAHIIDPGVDDYLH
                     QAADSANWPAGIVYTPNGDGIPMPKSLGVHEHWNNPADKLYSRNLGKDSGIELVKIFQ
                     NG"
     gene            complement(2874..3938)
                     /locus_tag="PK288_03825"
     CDS             complement(2874..3938)
                     /locus_tag="PK288_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18081.1"
                     /translation="MKKLVAAVLTGMVNMCLFSQNVVQPPSVNKTDWWGDIDGFLNQQ
                     AKITLDLASDALKLNPPALQENLTRKMALLMIDNVLHEEKAQFRPAVQSFYKERIENA
                     IREISASKVNSGALIWKLYNHTFIIKTPSATIGFDIQRGIPGNEDFAFSKELIGNLIN
                     VVDILFISHYHNDHADDWVAGAFIAQNKPVITPPGLWEQLPLYAAILHPDRSTDKIHD
                     IYLPHKQLNLKVVACPGHQGADILNNVYLVFTPEGMCFTHTGDQSNADDLKWIDKIGD
                     SYHIDVLMMNSWSYFPESRLAKGFRPRLIIPGHENELNHSIDHREPFWLNYIRLGDDP
                     AYPWIQMAPGEKYHYVAAGK"
     gene            4141..4749
                     /locus_tag="PK288_03830"
     CDS             4141..4749
                     /locus_tag="PK288_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18082.1"
                     /translation="MKKLLFLLLIFPVTGLAQTGKDIFNPEVPMVFFGIDFTKVQFTR
                     ADDFTNKPEILRFFVDANNLIDHGSLRNQVSRQLERKSVEWDLSYVTAGNSVVDWRNV
                     YSDNIDYVVPEDELNNMVKNLKVDQKKYNDFIGMVLIEENCCKAKPLQTITCLFFRVN
                     DLTVLFAERYKMKPGGIGFMNYWGVNHNMILARIGRLKKAVG"
     gene            complement(4832..5743)
                     /locus_tag="PK288_03835"
     CDS             complement(4832..5743)
                     /locus_tag="PK288_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18083.1"
                     /translation="MTAMHPFLFIIMSLLVSCSVKGDPNPGKDSPAEILFTEDFESTD
                     LASKGWYDNTNPAFSTTDNLPGSSRSLQMRFLKGGTAPVQGGAMRKLFDESGTLYVAF
                     NVKYSQNWQGSGVSYHPHEIHVLTNKNDTWSGPAFTRLTTYIENSAGKPRVALQDGQN
                     IDQARIGVNLVSTTENRSLMGCNGDSDGYGKGDCYDAGGGTYNNGKVWTASGKVISRG
                     EWHRIEVFYRLNSVVAGKGVADGIMKYWLDGELIFDLDHIMYRTAQNHDMKFNQLVIA
                     PWIGPGSPIEQTIWYDNLVVATSKPEN"
     gene            6086..6877
                     /locus_tag="PK288_03840"
     CDS             6086..6877
                     /locus_tag="PK288_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HPM18084.1"
                     /translation="MKQQKIFHAGNLILSCLLILFLTHCAHHTPVVGLDNWFNHEINA
                     ETGQPFHYLWTDREFSGYSRWGEIFTSSGAEITTVGKPLKDVLSKIDVYIIVDPDTTS
                     ESKSPNYIGDDDIRVITEWVEKGGVLAVLANDAPNCEFTHLNKLMRNFGMSFNHVTLH
                     PVTGTDYEMGASTQLPDHPLFKGVDKIYMKEISDINISGKAKPVLVEEGKVLVAEVNY
                     GKGYVFAIGDPWIYNEYIDHDLLPESFQNRKAAENLTNLLLGKVK"
     gene            6967..8235
                     /locus_tag="PK288_03845"
     CDS             6967..8235
                     /locus_tag="PK288_03845"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPM18085.1"
                     /translation="MKKFSYIQIAALLLLSLSCTEKEVKQLIAGSPESVKVSSERLGR
                     IDSMLIGSIREGWIAGAVGFIARDGKIVYDRAFGVSDIKSGTPMKTDDIFRIASQTKA
                     ITSIGLMMLFEEGKFLLDDPVSKYIPEFSNPVVIEKYNPKDTTYTTVPANREITIRNL
                     LTHTSGIEYAVIGSPMMRAIYAKGGITGGFGNDKITIGDDIRLLGKLPLLHNPGERYT
                     YGLNVDVIGYLIEVLSGKNLDKFLRERIFEPLGMADTWFYLPASKKGRLVEVNTEDAE
                     GRVKSMPQEAENYPLLEGTYFAGGAGLSSTVKDYAIFLQMLLNKGEYNGRRLLARRTV
                     ELITSNQIGELDLDKNKFGLGFQITTEEGQAQLGVSEGSFSWGGYYGTAYWADPKERL
                     VGLIFLQQYPLSHGEIADKFKAMVYQALTD"
     gene            8421..9479
                     /locus_tag="PK288_03850"
     CDS             8421..9479
                     /locus_tag="PK288_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HPM18086.1"
                     /translation="MSKLFSTLAVKKIILRNRIVMSPMCQYSSSDGFANDWHLVHYGS
                     RATGGAGLVIVEATAVSPEGRITPGDLGIWSDEYITGLERIAGFIHSKGAVAGIQLAH
                     AGRKGSCAVPWKGGKQLGLDKGGWQTIAPSEIPYFPDDRIPRPLDTDDIGKLISAFRA
                     AAKRARMAGFRVIEIHSAHGYLLHEFLSPLSNRRTDEYGGGFQNRIRFLTRVVEAVRS
                     EWPAENPLFVRISATDWTEGGWSLEDSIQLASVLKGLDVDLIDCSSGGNIFDAVLPLE
                     PGYQVPFSAEIRKTGILTGTVGLITQAGQAESILQEGKADVVLIGRELLRNPYFPFHA
                     ARSLGAEHEWPVQYLRAK"
     gene            9557..9916
                     /locus_tag="PK288_03855"
     CDS             9557..9916
                     /locus_tag="PK288_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPM18087.1"
                     /translation="MSKEDPVRIPCVEEDKVIAVRARMKSEQFFHYLAETFKALSDPT
                     RTKIIFALCQEEELCVRDIAAIINTTNSAISHQLRTLRNMRLVRYKKVGRVTFYSLDD
                     IHINNLFAEGLRHVEEK"
     gene            9973..10227
                     /locus_tag="PK288_03860"
     CDS             9973..10227
                     /locus_tag="PK288_03860"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPM18088.1"
                     /translation="MNSDRGEMSLIEVEDGQTGIIVSVVGGKNLTKRLADLGLVSGTE
                     IKVIRKALFSGPLQVEVSGSRLVLGRGLASKIMVLSNGRK"
     gene            10214..12151
                     /gene="feoB"
                     /locus_tag="PK288_03865"
     CDS             10214..12151
                     /gene="feoB"
                     /locus_tag="PK288_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPM18089.1"
                     /translation="MAENNQKINIVLAGQGNAGKSAVFNYLTGLHQHIGNWAGKTIEK
                     REGTLFYKDHTVDVLDLPGIYSLTTYSEEEQISREYIINQQPDFVINIIDSTNLERNL
                     IFTLQLLELGSPLILALNMVDLAKKKGVEVNVGKFQEKLGVPVISTVASRGLGMTRLM
                     DKVIELNGVGKNNHMVPEYGKEIEGCIAELSGLLRNSEYRYPVRWIAIKLLEKDQEIT
                     KLIEKSQPEIIDRSVQLTEYLEDIHGHDSSIVIADERIHLVAHIIRETVKFTPRAILS
                     FNEKLDSLTTHKFTGYLLMTVILGGMFLSVFVLGNWISDWLDGVFSSWHSWWESAVGT
                     TFASLLGWSAIESVLALIQIALPYILPFYIILYILEDWGYISRISFLMDNLMHKMGIH
                     GKGCISFVIGFGCNVPGCLSCRIMESKRERFITAVLVTLVPCSAVSVIIFGLVGKYVG
                     LLWAFGLYVFAMVVIFIAGRLSTKVVGGEPVELIMPMPDYKTPHMRTILVQTWDALKE
                     FIYIAAPIVIVSGVVIQAINLAGWMPAISSVLSPITVNWLGLPAITGILLIFGILRKE
                     LILVMLAAFLGTANFGEVLTAQQMLTLSVVSMFYIPCVATISVLWNEYGWKRAMAVCV
                     LEILFAIILGGIVSRLLNFVF"
     gene            complement(12165..12593)
                     /locus_tag="PK288_03870"
     CDS             complement(12165..12593)
                     /locus_tag="PK288_03870"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM18090.1"
                     /translation="MEIKEVKRFSLRVFNSVSRLLPQLSPDIRPLTREHFRAIIRQKS
                     VHLFTAENAGKIVGMLTLVIYRIPAGTRFWIEDVVVDNSERGKGYGKELTQFALDFAE
                     HSGAETIELTSRPSRVAANKLYQTMGFRIRETNVYRYTLK"
     gene            complement(12619..13044)
                     /locus_tag="PK288_03875"
     CDS             complement(12619..13044)
                     /locus_tag="PK288_03875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPM18091.1"
                     /translation="MKNDLIRNIENDRFARLTGIVLSEVREGYAAAELALNENHLNGL
                     NQVQGGVIFTLGDFVFAAASNSGGTTTVGLNVNITYFSSPTGKILRATAREIRVQNRI
                     SGYNVEIFDEDGTLVAVFSGLGYRKPSPGQAVSGENHPR"
     gene            complement(13273..14265)
                     /locus_tag="PK288_03880"
     CDS             complement(13273..14265)
                     /locus_tag="PK288_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18092.1"
                     /translation="METRDNKTGNDLNRTGENLVVKNDLRNARREGITRGALITGLIS
                     VFFIIGLGLILYSVYKRDHNSQLTLMENQKLLFTEQLNERDSMINDWLNTFDEIERNL
                     NEIKQKEKIINVQASDSEFPKEKKEQVLADIKYINTLIEENKKKIAQLNAQLKKSGNT
                     IAGLQTRITDLEATMKQYETQIAGLKATLVEKDFQIEQLNTQMFALQDTLVQKTETIN
                     SQTDKLNQAYLATGTYKDLREKGLVSKEGGFLGLGRKESLIEDFSDSLFDRIDVTKTR
                     TIPVNSKKAKLITEHPTDSYQMIRENENTIAYIEIKNPSEFWKISKYAVVQVVK"
     gene            complement(14446..15804)
                     /locus_tag="PK288_03885"
     CDS             complement(14446..15804)
                     /locus_tag="PK288_03885"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM18093.1"
                     /translation="MNERPVRIFVVEDNEWYNKLLVYTLSLNPDYEVRSFFNARDFLN
                     SLGESPDIVTLDYRLPDLSGLEVLKKIRQENSDIQVILISEQEDIDMVVTLLKLGAYD
                     YITKSDDIRERLLNTVQNIRNDLGLKSQIRNLRKEVQKKYNYREGILGESPAIRNVYE
                     LIEKAVSTNITVIVSGETGTGKELVAKAIHYNSNRKDKAFVAVNVPAIPSELIESELF
                     GHEKGAFTGAAFRRIGRFEEADGGTLFLDEIGEMEINLQSKLLRVLQEKEIVRIGNNK
                     PVKTDCRIIAATNKNLREEVKKGNFREDLYYRLLGLQIEMPPLRERGNDLLILARHFI
                     RDFCSVNNIPEKRLSAGAQKKLMAHSFPGNVRELKSVVELAVTLSVNEEITADDFNLD
                     SDDSPATRTDENLTLREHEMKIVKDTLRRCNNDISLAAKKLDIGISTIYRMLKQEKES
                     ST"
     gene            complement(15873..18287)
                     /locus_tag="PK288_03890"
     CDS             complement(15873..18287)
                     /locus_tag="PK288_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM18094.1"
                     /translation="MTGKFKKYLKRNSIQIKIGLLMTISVILLTVTSFLLYRNLSSIV
                     SSIRIDFNPELRLQSIRDISMDLGKAGNSVRIYTITKNPSDIKSYYDIISNIDGKMSV
                     FREECRNDSSLLVQADTISRLIEENIYIWNQLLTLYKDNMVIDYLKQLSDELNVAATA
                     ADQRRGILKRVFSRAPQKTTDEKEIIEDINEVVRKTSTAQNELLEQESQLAVNSSIIT
                     GKFYDLITKMENQVFQQIRAREDAADQIAEKTFRLLVLLSISGGLLAILVLYIIIRYA
                     RNAYAYQVALEKSKDEAERLSRTRELFVANMSHEIRTPVTAISGFTEQLLHDPLNETI
                     TDSLKIIKSSSDHLLRIIDDILDFSRLHNNKLTLEKIHFSVGRITDEVYALFEKQARN
                     NNTMLSFSISPVTPPVLLGDPFRLKQIMINLVSNSVKFTRNGSVHFSIDSIIKQNEEL
                     ELLMEFTDTGIGIDESKIDVIFEDFTQAEMSTTRKYGGTGLGLSIVKRLVELHNGKIE
                     FKSRKNQGTKIICRLPYLTGDEKELKNEMPPSPAIPGRLRGMKFLVVDDEEYNRLLFK
                     KVFDRWNISYAESPSGVDALELLKEERYDLLFLDMRMPGIDGLKTARFIRDEMDIKES
                     DMPIIIVSAAPPDDWEKYRKAGINAFLQKPFSEEKLLTAVLNVIGGYTADEVRGNSAG
                     KSEERPRSEKINLKNLKHIAAGDEQFVNQMLESFLVSTEKLLDEMQAAARLSQWRAVS
                     DLAHKMIPPCRHIGAMDLCNILTAVEKTAGNNDIPDSAETMIPKALGEFEIVRNLLKE
                     QIEKFN"
     gene            complement(18284..18718)
                     /locus_tag="PK288_03895"
     CDS             complement(18284..18718)
                     /locus_tag="PK288_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18095.1"
                     /translation="MKQKINVLVIEGDEYYNNLLSNAIQQSVNPFLEKGKYQLILRSF
                     TNAGDFIRKIKSGELECNNSAVFINYHLGEGITAPQVIGLMMELTGDPMVVLLSQSKT
                     VREKIKQVPHDYFVVKDRFAPALCSLYMQQYIENKYSVFLDA"
     gene            complement(18954..20318)
                     /locus_tag="PK288_03900"
     CDS             complement(18954..20318)
                     /locus_tag="PK288_03900"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPM18096.1"
                     /translation="MITLSKNFYPVLIILLFLTGVKNPGHCQPADMKKVAAEMEDLLL
                     KNMMPVWYPRVIDKEYGGYLSNFNYKWEMTGSQNKMIVTQSRHVWACSEMKAFTGDPV
                     YLDYARHGFLFLRDHMWDKQYGGFFNLVSREGTPLKEADPKQFAKNAYGNAYAIYGLA
                     AYYRESRDPGVLELAKEAFAWLEKGSHDPVYGGYFQFLNQDGTPIREGGPGGTPPKDQ
                     NSSIHILEALTELYHVWPDDLVRKRLDEMLHLIRDVIVNDKGSLQLYLNADLTPVSYQ
                     DSSKKVHLKHYALDHMSFGHDVETAYLLMEASEALNSVADSKTLEIGKKMVDNSMNYG
                     WDNSNAGVYDVGYFYKNEKSVTIIRDTKNWWAQSETLNTLLIMSELYPDDPLNYRKRF
                     FDQWKFIKDYLIDWQHGDWYSGSTDKEPRAKEGTKANIWKGNYHTTRALINCITRIRE
                     MGKK"
     gene            complement(20421..24107)
                     /locus_tag="PK288_03905"
     CDS             complement(20421..24107)
                     /locus_tag="PK288_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPM18097.1"
                     /translation="MKSTKAGCFLLGLLFFAISVPGCHKKNIRYNRGTGIYPGDLRED
                     FSPDLAPDRTTYRNLARLRPAFHSSSYDYNLTAQLVTDGIFTHEIPDHISVSTCSGVA
                     PINEREWLLDHNSATEVNLEGSEVWIEFSINREAKPIIADRICLNGSMIYNEKKTCGW
                     KFVCLGSDDGINWEEISEIRGAGLPGEEKRDPSAAMINQQGQKKRNISVKRSGTFPPS
                     PYGPDSIAPRPSFQTSSGSGSEPMQRLINPVFTPVGPVAWHYFRIILTSPCAEKWTIS
                     DLDFYLGGSRLEVTPSHNFKSAWMSAGNEREWIYVDLGSASSFDRIRLHWINKALGGS
                     VQVSDDSERWKDVAPLPGHDGRIDDLPVKDGRGRFVRILMEKPESGNKFVLSELEVFG
                     TGGLATRPKAPSPAGHGRLFLNGGNWKVQRASEVSATGQIISSEGFDCKDWVDATVPA
                     TILVSYINAGAVADPDFGDNQLLISESFFNSDFWYRNEFHLPEHFRGKRLFLNFDGIN
                     WKADVFVNGKEAGKIDGAFIRGRFDITSLIVPDHVNALAVLIRKNENIGTVKEKTALS
                     ADKNGGSLGADNPTYHASIGWDWIPTIRGRNTGIWNDVFLSTTGDVSIIDPFILPDLP
                     LPDTSSADLFVEVTLKNHSRERIKGILSGKYGDVSFETDVVLAGSETKLIKLNPSTHP
                     VLRIENPRLWWPRGYGNPHLYDVELSFKTENGEFSDHTSFKSGIREMYFTENYQTLNM
                     YINGRRFTGRGGNWGFPESNLRYRGREYDAAVAYHADMNFTMIRNWVGQTGDDEFYEA
                     CDRHGIMVWQDFWLANPADGPDPADPGMFMRNANDLVKRIRNHPSIGLYCGRNEGNPP
                     VILDTALKSMIRRLHPGLHYISNSSSGVVSGGGPYKALPVRDYFLLFGYGKFHSERGM
                     PNVMTWESLGLTLPENALWPQNRLWGMHDYCLESAQNASTFNQMVEKGFGPVTDGMKF
                     TGLAQWINYDGYRGMFEGRSMFRQGLLLWMSHPAWPSMVWQTYDYYLEPTAAYFGCKK
                     ACEPIHIQWNPVFNDIEVVNYSGGNKASLTAKAIIMNMDGSVEWNKATIITSREFSTV
                     TCFRLEFPENLSDVHFIKLTLADGDQLLSENFYWRGKDEGNFRALNNLPLVHLKMSTR
                     TKKSGGNWNLTTTLSNPTPHPALMVRLKIVGRSSGERILPVFYSDNYIFLMPGEEKTI
                     DMKLKDADTRGEKPVVEISGFNVK"
     gene            complement(24218..24685)
                     /locus_tag="PK288_03910"
     CDS             complement(24218..24685)
                     /locus_tag="PK288_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18098.1"
                     /translation="MKNAVFLILLLTVSLAASAQETDFSGKWKLNTGKSKLNEDFSSA
                     PFQVVITQDGNDMTIERHQDFRGETSVMTDKYTLDGKECQNKAFMESVKTSTAKWSED
                     GKSLKVSSKIVQEDGNETIITEIFKFDGDNLVIDYVRAVFWGETEETFVFDRI"
     gene            complement(24929..25378)
                     /locus_tag="PK288_03915"
     CDS             complement(24929..25378)
                     /locus_tag="PK288_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPM18099.1"
                     /translation="MSIAEKIASDLINAMKGKDKVALEAIRAAKTAFLLAKSEKGTDA
                     VLSTEDELNIVRRLVKQRRESAAIYREQNRTDLYEKETAEADVLEKYLPAKLSDEELT
                     EIIKMVISRLGATSPAEMGKVMGAAVRELAGRADGKEISAKVKQLLG"
     gene            complement(25634..>27237)
                     /locus_tag="PK288_03920"
     CDS             complement(25634..>27237)
                     /locus_tag="PK288_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPM18100.1"
                     /translation="LVSGNITETGFILSWTASSDNVGVTSYEVYRNGSLAATVTGTSA
                     NITGLLPSRTYSMTVRAVDAAENRSAASSPLSVTTIDTHPPSIPAGLVATGVTETSFT
                     LSWSASSDNVGVTGYDIYCNGVLSTSVNGITASFTGLTLLTNYSMTIRAKDAAGNISN
                     HSNALVVRTPDTHAPTVPTNLVATKISETSFTLSWSASTDNSSVAGYDIYRNGNLFTS
                     VTGTSAIISGLSPASTYSMTVRAKDASGNISPLSDALRVNTNDTHPPSVPANLKASNL
                     SETSLTLNWSPSTDNVGVSKYEVYCNDILLATTTTSKADISGLTVFTDYNLTVRAFDA
                     AGNVSAFSQILKVKTPDLHPPTAPENLSVKNLQNSGFSLYWTPSTDNVGVSGYEIYCN
                     DMPVITLKESEFLFVNLSDSIKYEITIVAVDESGNTSDPSLPFIIKLADYFPLTKEQL
                     GSIVIYPVPVRGNEFYVDLGREFIGEMNLVIYSLKGTILYQQKVHCSGEILTISNPIT
                     KNGMYLLSIGNNITRITRTIIINRE"
BASE COUNT         7004 a         6029 c         6234 g         7970 t
ORIGIN      
        1 acagggtatg ttgacagggc ctttcccatc agtgatcaga ggtaaccccg gtttttaaaa
       61 atgatccact ggtaaacagg taatacgaat caggtttcta aagtttggaa tgttctgaag
      121 gttccctata tttccatcag gaaagaataa ttgaaaagca taaacaggtt tgttgaagca
      181 actcaatttg tttcaatagc atggcacctt aggcggtttc atttcacctc acctgcggtc
      241 ccctgcaggt aaacattatc attgtgttat tgttattctg ttaacaatag gaacagtaaa
      301 cccggaggta tggaaagaca aattcataag cgtaaggtag taataacagg agccggttcg
      361 gtgggggcaa cctatgcata tgcacttgcc cagagcggtg ttgccgaaga gatagttctg
      421 ctcgacagga atgaagaact tctgaaaggg caggtccttg acctggttca cggtcagcct
      481 tttttcccca cagtttcgat aagggccggc aatgatgacg attatgggga tgcgagcctt
      541 gtggtgatta ctgcgggatc ggcccagaaa ccgggtgaaa cgagattgca gcttttgcag
      601 aagaatgcag ggatagtagg cagtatttcc gaaactgtgg cgcggagcgg ctgcagggga
      661 gttatgctga ttgttaccaa tccggtcgat gtgatgacct atgttgcatt caggtattcg
      721 agatggtcaa ggaacagggt gatcggctcg ggtaccgttc tcgacagcgc aaggctgagg
      781 cactttatca gcctgcattg cggggtggat ccgcacagcg ttcatggata tgtcctgggt
      841 gagcatggtg acagtgagtt cgcagcctgg tcaatgattc atatcggggg aattccgata
      901 gacaaatact gcagtcattg cggaaaatgt gaaggttggg atgaacagaa gaaattgtgg
      961 gaacaacagg tccgtgattc ggcatatcat ataattgatg ccaagggttc cacttactat
     1021 gcagtgggac tggccatggt caggattaca tcatccatac tccgcaatca gaacagcatg
     1081 cttaccgttt cggtgatccc tgacggcgaa tacggattaa aaaacctcgc aataagcata
     1141 ccgtcaatag tttcgagtgc cggagttgca gggatcactt ccgtgccgct gccccaggat
     1201 gaacttgaaa aattatataa ttcagcctct gtgatcagaa aggccattga agagacaggg
     1261 atatagatca gcctgccggc ttcagccgtt ctggaatatt ttcacaagtt caataccgct
     1321 gtccttacca aggtttcgtg aatatagctt gtctgccggg ttattccaat gttcatgaac
     1381 gccaaggctt tttggcatgg gtatgccatc gccgtttgga gtataaacaa ttccggccgg
     1441 ccagtttgcc gaatccgccg cctgatgcag ataatcatca acgcccggat ctataatgtg
     1501 agcaacggta tgctcaggat atgagaactc ggttcttaga aaatcatatg ccaccgattc
     1561 aatggcaacc ggatcaagtg aaaggagcag tgatgagctc cagtgattgt taaagggcgg
     1621 catcaggaat ttcacaggaa gcccgttcca gtccgtacca gaccagagag catcgagtat
     1681 gtagaaaaga tttttgtcgc cggtatattt attgcccatt agatcgacaa gcaccctgta
     1741 atgcccgtat cccttgcggt tgccgccggt gttaaggccg ggatggagat gagccgcact
     1801 tcgtctcgac tgcgtcccga aatgattttt tgcgcaaagg gtgatgccgg ccaatccatg
     1861 tcctttcatt gctgcaaggt taatcaggta ttcagcttct tcaagcacag tgtaaatctt
     1921 atccgacccg gcattaccca ttactgactt cctgtctgaa tagaaaataa ggtccttttt
     1981 gctttcaccg caggttattc ttccatgggt tgcacccatg ctcgaaagat aattcacacg
     2041 gggaaactcc gaatggtatt tgatatactc atcctggtaa atattccgca ttgcgtctcc
     2101 tacgtagatg gactcctggg gaacaccagc cacgttaaca agctgacgca gcattgagat
     2161 cacaagatgc ggcgatgttt cacaggcaag aggcagcagc ttttccttcg gcctggcgta
     2221 atcaggaccg atttcccctg aagcagtggt cctgttcagc tttatgtaaa tcttttctcc
     2281 cttgctatag ggaacgcttc ccttaccgcg cttccggtta tgaaagcgga atacggcatc
     2341 ccaggcatca ttaacatttt cttttccagt catcgaacag agtccggcca ggagcatttt
     2401 atccagtatc ttctgatcgg tgttgttatc catgaaccat gcatcgtatt tttccgccgg
     2461 gttggcaccc gtaacaggtt tattggcaca ggctggattt gtacaggaag ggttccacac
     2521 ccagctgacc ctgccgggca ttataccctg gcctgtcccg acgggccggt tcgggatata
     2581 aagatcttca ggcagcagat ccggcagcca ctggtcggtc ccccctttga gattgttttc
     2641 ccatggaaga tccctgacat ttccctgggc cctcacggct gacaggaaca ggctccactg
     2701 aaccggtacc agtatcaggc ctttcagaac tttgcgcctg ctgatcatat ttggccgtga
     2761 tccggctgaa gcgccagact gcgatccttt cttcgaagct ttgttctttt ccatttcagg
     2821 taatttagat aatgttttgc cttgctcaaa aatacaaaaa atcactgcag ttgttatttg
     2881 ccggcagcaa cgtaatgata tttctccccc ggggccatct gtatccatgg ataggccgga
     2941 tcatcgccca gcctgatata attcagccag aatggttcac ggtgatcgat gctgtgattc
     3001 agttcatttt catgccccgg tattatcagc cgtggcctga aaccctttgc cagccttgat
     3061 tcggggaaat atgaccaaga attcatcatg agcacatcaa tatgataact gtcaccaatt
     3121 ttatctatcc atttcagatc atcagcattg gactggtctc cggtatgggt aaagcacata
     3181 ccttcaggcg taaatacaag gtatacgttg ttcagaatgt cggctccctg atggccggga
     3241 caggcaacca ctttgagatt caattgctta tgaggcaggt aaatatcatg gatcttatca
     3301 gtgcttctat cggggtgcag gatagcggca tacaggggca gttgttccca gagcccgggc
     3361 ggtgttataa ccggcttgtt ctgtgcaata aatgcgccgg caacccagtc gtcagcatga
     3421 tcattatgat aatggctgat gaaaagaatg tcaaccacat tgatcaggtt gccaatcagt
     3481 tctttactga atgcaaagtc ctcatttccc ggaatgcctc tctgaatgtc gaatccaatt
     3541 gttgccgacg gggtttttat gatgaaggtg tgattgtaca gcttccagat cagggcgcct
     3601 gagtttacct ttgatgcact gatttcccgt attgcatttt caatcctctc cttgtaaaag
     3661 gactgaactg ccggcctgaa ctgggctttt tcctcatgga gaacattgtc aatcatcagg
     3721 agggccattt ttcgtgtcag gttttcctgc agtgccggag gattgagctt taaggcatca
     3781 cttgcaaggt ccagggttat tttggcctgc tgattcaaaa aaccgtcgat atccccccac
     3841 cagtcggtct tgttaacaga cggaggctgc acgacgttct gcgagaaaag acacatatta
     3901 accatgcctg taagtactgc tgcaactagc tttttcataa tcaagttcca taaaatttcc
     3961 cgaccgtgat attgctccgg ttaaaaggca agttataaaa aatcgggagc tttggcaatt
     4021 atcaggttaa ttcgttagaa tggatgaact gacttgcact gaagggtaca attccgatcc
     4081 atggaataga gtctttgtaa taatttttga tttaatttta ttgaacaaaa tacttttcaa
     4141 atgaaaaaac tactttttct tttgctcatt tttccggtga cgggtttggc ccagacaggg
     4201 aaagatattt tcaatccgga agttccgatg gtcttttttg gaattgattt tacaaaagtt
     4261 cagtttacca gggcagatga ttttaccaat aaacctgaaa ttctgagatt tttcgtggat
     4321 gcaaataatc ttattgatca tgggtcattg aggaatcagg taagcagaca gcttgagagg
     4381 aaatcagtag aatgggatct ttcgtatgtc accgccggca actccgtggt tgactggaga
     4441 aatgtgtatt cagacaatat tgattatgtt gttcccgaag atgagttaaa caacatggtg
     4501 aaaaatctga aggttgatca gaaaaaatac aatgacttca ttggcatggt tctgattgag
     4561 gagaattgct gcaaggcaaa accacttcag actatcacct gtttgttttt cagggtcaat
     4621 gatctgacag ttctttttgc ggaaaggtat aaaatgaaac ccggaggaat cggtttcatg
     4681 aattactggg gtgtaaatca taacatgata ctggccagaa taggcaggtt aaaaaaggcg
     4741 gttggctgat ttcagattaa gtattccctg accgtatatc ggttttttat ttttgaaggg
     4801 agggatttta aacaggtttg ttgtctatac cctaattttc aggtttggag gttgccacta
     4861 ccagattgtc ataccatata gtttgttcaa tgggagaacc tggtcctatc cagggtgcta
     4921 tgacaagctg attgaatttc atgtcgtgat tctgtgcggt ccggtacatg atatgatcca
     4981 ggtcgaagat aagctccccg tcaagccagt acttcataat accgtcagca acaccctttc
     5041 ctgcaacaac actgttcagc ctgtaaaata cttcaatcct gtgccattcc cctctggaaa
     5101 taacttttcc ggatgcggtc cataccttgc cgttgttata tgtgccgccc ccggcatcgt
     5161 aacagtcacc tttcccgtag ccgtcactat caccattgca acccattagc gaacgatttt
     5221 cagtggtgct gaccaggttt accccaatcc tggcctggtc aatgttctgc ccgtcctgca
     5281 atgcgacgcg cggttttcct gcactatttt caatataggt ggttaacctt gtgaaagccg
     5341 gtcccgacca ggtatcgttt ttgtttgtca gaacatgtat ctcatgcgga tgatatgaaa
     5401 cacctgaacc ctgccagttt tgactgtatt ttacattaaa tgcaacgtac agtgtgccgg
     5461 attcatcaaa gagttttcgc atggccccgc cctgaaccgg ggcagtacca cccttaagaa
     5521 accgcatctg cagcgaccgg ctgctgccgg ggagattatc agttgtcgaa aatgccgggt
     5581 ttgtattatc ataccagccc tttgaagcca gatcagtgct ttcaaaatcc tccgtgaaaa
     5641 ggatttctgc gggagaatcc ttgcccggat tcggatcgcc tttaacactg catgaaacca
     5701 gcagcgacat tatgataaaa aggaaaggat gcattgcagt catatatgtt taataatatt
     5761 tcttcatgca atataatatt ttgtttgtaa ttccaatgtg cgacaacttc ctgtttgcgg
     5821 ttgattttca accctggatg ccaccggttt atggaataat ctgttcaatt ctgaacagga
     5881 taatacttta atagtaaaga aaatccggga ttcctgattg catttacggg gtgtcctgtt
     5941 atccggattg taccggggcc gccggaaatc acataaattc agctggttaa taatgccggt
     6001 agcatttctt acggacaaat aattatcata cttccgggca actgtcataa tgttttttat
     6061 ttttgaataa attatttcag ggaccatgaa acaacagaaa atttttcacg ccggaaacct
     6121 tattctctcc tgccttctga tcctgttcct cactcattgt gctcatcata cgcctgttgt
     6181 agggctcgac aactggttca accatgagat aaatgccgaa accggccagc ctttccatta
     6241 tctgtggaca gacagggaat tcagtggtta ttcgaggtgg ggtgaaatat tcacttcctc
     6301 cggcgccgaa atcacaactg tagggaagcc gctgaaagat gtcctcagta aaatagacgt
     6361 ttatataata gtggatccgg atacaacgtc tgagtcaaaa tcaccgaact atatcgggga
     6421 tgatgatatc agggtaatta cggaatgggt tgaaaaagga ggcgttcttg cggtccttgc
     6481 caatgacgct cccaattgcg aattcaccca tctgaataag ctgatgagaa atttcggaat
     6541 gagcttcaat catgtaaccc tgcatcctgt taccgggact gactatgaaa tgggtgccag
     6601 tacgcagttg ccggatcatc ctctttttaa aggcgttgac aagatatata tgaaggaaat
     6661 ttcagatatt aatatttccg gaaaggcaaa acctgttctt gttgaagaag ggaaagtttt
     6721 ggttgcagaa gttaattacg ggaaaggtta tgtttttgcc atcggtgatc cctggatcta
     6781 taatgaatat attgaccatg atcttctgcc cgaaagtttc cagaacagga aagcagctga
     6841 aaatctgaca aatctccttc ttggaaaagt aaaatgaaga ctctgatgaa ttttcccgga
     6901 aattggttta tcatgataat tcttccatac accatatgtg tttaaccaag tctcaacaaa
     6961 ttatacatga aaaaattcag ttacatacaa attgcggcac ttctgttatt gtctttatcc
     7021 tgtactgaaa aggaagtaaa acagcttatt gcaggatcac ccgaatcggt taaagtttcg
     7081 tctgaacgac tcgggcggat tgattcgatg cttatcggca gcatcaggga aggatggatt
     7141 gccggggctg tcgggttcat tgccagggat gggaagatag tctacgacag ggcattcggc
     7201 gtgagtgata tcaaatccgg gacacctatg aagacagatg acatattcag gatagcctcc
     7261 cagactaaag ccattacaag catcgggctg atgatgttgt ttgaggaggg aaaattcctc
     7321 ctggatgatc cggtgtcaaa gtatattcct gagttttcaa accctgttgt cattgagaag
     7381 tacaacccga aggatacaac atatactact gttccggcca acagggaaat taccatacgt
     7441 aatctcctga cacatacctc ggggattgaa tacgcggtga taggatcacc catgatgcgg
     7501 gctatttatg ccaaaggcgg gataacgggc ggtttcggga atgacaaaat aacaatcgga
     7561 gatgatatca gattgcttgg aaagttacca ttgcttcata atccgggaga aagatacact
     7621 tatggattga atgttgacgt gattggctat ctgattgaag tcctttcggg caaaaacctt
     7681 gataagttcc tcagggaacg aatttttgaa ccattgggga tggccgatac gtggttttat
     7741 ctccctgcct caaaaaaagg caggctggtg gaagtgaaca ctgaggatgc tgaaggcagg
     7801 gtgaaatcaa tgccccagga ggcggaaaac tatccgcttc tggaaggtac ttactttgca
     7861 ggaggagccg gtctgagttc aaccgtgaag gattatgcaa tattcctcca gatgctgctg
     7921 aacaagggag agtacaatgg cagaaggctg cttgcccgcc gcaccgttga gctgattacg
     7981 tcaaaccaga taggggaact cgatctggac aagaacaagt tcggactggg tttccagatc
     8041 acgactgagg aaggacaggc ccagcttggt gtctcggaag gttcattttc atggggcgga
     8101 tattacggaa ccgcttattg ggctgatccg aaagagagac tggttggtct tatcttcctg
     8161 cagcagtacc cgttaagcca cggcgaaata gctgacaagt tcaaggcaat ggtttatcag
     8221 gccctgaccg attagacgga agtgatggca gatcctgaaa gccgataata accggtgaaa
     8281 gtaaatgaat gatatttata ctgctgaaca tgtaccaggg aagtggcggg aatctcagcc
     8341 gtaagtgaca gatatccctg attttttgta cagttgataa ttctatagaa caaggtagat
     8401 tattatttta aaaagatccg atgtcaaaac ttttttccac tcttgccgtt aagaaaataa
     8461 ttctcaggaa ccggattgta atgtcgccga tgtgtcagta ttcctcatcc gacggttttg
     8521 ccaacgactg gcaccttgtg cattacggaa gccgggcaac cggcggtgcc gggcttgtta
     8581 tcgttgaagc aaccgccgta tctcctgaag gaaggattac tcccggtgat cttggaatat
     8641 ggagtgatga atacataacg ggattggaac ggatagcggg gtttattcac tcgaaaggtg
     8701 ctgttgccgg tatccagctg gcacatgccg gaagaaaagg ttcatgtgca gtgccctgga
     8761 aaggagggaa acagctgggg ctggacaaag gcggctggca aaccatagca ccatccgaaa
     8821 ttccctattt cccggatgac agaattcccc ggcctctgga tactgatgat atcgggaagc
     8881 tcatttcagc gttccgtgct gcagcaaaaa gagcccggat ggccggattc agggtaattg
     8941 agatccatag cgcccatgga taccttttgc atgaatttct ttctcccctg agcaacaggc
     9001 ggactgatga atacggagga ggatttcaaa accgtatccg tttcctgacc agggttgttg
     9061 aagccgtgag atcggaatgg ccggcagaaa atccgctgtt tgtcaggatc tcggcaaccg
     9121 actggacaga aggcggatgg agtcttgaag acagcattca gcttgcttct gttttaaaag
     9181 gtctggatgt cgatctgata gattgttcat ctggcggaaa catttttgat gccgttttgc
     9241 cgcttgaacc cgggtaccag gttccattct ccgcagaaat cagaaaaaca ggaatattga
     9301 cagggactgt cggacttatc acccaggccg ggcaggctga atcgatcctt caggaaggaa
     9361 aagccgatgt ggttcttatt ggaagggaat tactccgcaa tccttacttt ccgtttcatg
     9421 cggcacggag cctgggagcg gaacacgaat ggccggttca gtatctcagg gcaaaataaa
     9481 tccataaata gatttaaatg aaagaaaaat tatatttttg catgataatt gaataattgt
     9541 tcatatataa tattgaatga gtaaagagga tcctgtcagg ataccgtgcg ttgaggagga
     9601 taaagtcatc gctgtcaggg ccaggatgaa atcggaacag ttttttcatt acctggctga
     9661 gacctttaag gcactgagtg atcccacacg aaccaaaata atatttgccc tgtgccagga
     9721 agaggagttg tgcgttcgtg acattgctgc aataataaat accacaaatt ccgcaatatc
     9781 acatcagttg cgtaccctga ggaatatgag gctcgtaagg tacaaaaagg ttggaagagt
     9841 gactttttac tctcttgacg acatacacat caataatctg tttgccgaag gactcaggca
     9901 tgttgaggag aaatgatttt tttagaaata ttattgaata attgctcata tatcaacata
     9961 taataaaata atatgaatag cgacaggggt gaaatgagcc tgattgaggt agaagacggt
    10021 caaaccggga taattgtttc ggttgtcgga gggaagaacc ttacaaaacg gcttgcagat
    10081 ctgggcctgg tatcaggcac tgagataaag gtaataagaa aggctctttt ttcgggcccc
    10141 cttcaggttg aagtgagcgg ttcaaggctg gtgctgggca ggggcctggc atccaaaata
    10201 atggtactat ccaatggcag aaaataatca aaagataaat attgtactgg cgggtcaggg
    10261 taatgcggga aaatcagctg tattcaatta tctgacgggc ctgcatcaac atattggcaa
    10321 ctgggcaggt aaaaccatcg aaaagcgtga gggcacgctg ttttataagg atcatactgt
    10381 tgatgtgctt gacttgccgg ggatatactc acttaccact tattcagaag aagagcagat
    10441 ctcgagggag tatatcatca atcagcagcc ggattttgtt atcaacatta ttgactccac
    10501 caatcttgag cggaatctga ttttcacgct tcagctcctg gagctgggaa gtccgcttat
    10561 acttgccctg aacatggttg acctggcaaa gaaaaaagga gttgaggtca atgtgggcaa
    10621 atttcaggag aagctggggg taccggttat ttcaaccgtg gcttcgaggg gactcggcat
    10681 gacacgcctg atggacaagg ttattgaact taatggtgta gggaagaata accacatggt
    10741 acctgaatac ggaaaggaaa tagaaggctg tattgcagaa ctttcaggcc tgctcaggaa
    10801 ttcggaatat cggtatcctg tcagatggat agcgataaag cttctggaaa aggatcagga
    10861 aataacgaaa ctgatagaaa aaagtcagcc tgagatcatt gacagatctg ttcagcttac
    10921 tgaatacctc gaggatattc acggacatga ttcctccatt gttattgctg atgagaggat
    10981 acatctggtt gcgcacatca tcagggaaac ggtaaaattt acaccacggg ccatattgtc
    11041 attcaatgag aagcttgaca gcctgactac ccataaattc acgggatatc tgttaatgac
    11101 cgtgatactg ggcgggatgt tcctgtcggt ttttgttctc ggcaactgga tctccgactg
    11161 gctcgacggg gtgtttagct cgtggcacag ttggtgggaa tcagctgtag gaacaacctt
    11221 cgcatcattg cttggctggt cggcgattga aagcgtcctg gcccttatac aaattgcgtt
    11281 gccatatatt ctgccgtttt acattattct gtatattctt gaagactggg gttatatttc
    11341 gcgtatttcc ttccttatgg ataaccttat gcataaaatg ggtattcacg ggaaaggatg
    11401 tatttctttt gtaataggct ttggctgcaa tgtgcctgga tgtctttcct gccgtataat
    11461 ggagtcaaaa cgcgagaggt tcataacagc cgtgctggta acgcttgtcc catgttcggc
    11521 tgtttcggtg attattttcg gccttgtagg gaaatatgtc ggattactat gggcattcgg
    11581 attatatgtt tttgccatgg tagtgatttt cattgcaggc aggttatcca cgaaagtagt
    11641 cggaggggag cccgtggaac tgatcatgcc aatgcctgat tacaagacac cgcatatgag
    11701 aacaattctg gtgcagacct gggatgccct taaggaattc atctatatag cggcacccat
    11761 tgttatagta tccggggtgg tgatccaggc tatcaatctt gcgggctgga tgccggcaat
    11821 atcttccgtt ctgagtccca taaccgtcaa ctggctcgga cttccggcca ttacaggtat
    11881 tcttctgatt ttcgggatcc ttcgcaagga actgatccta gttatgctgg cagcattcct
    11941 cggcacagca aacttcggtg aggtgctgac agctcagcag atgctgacgc tttcagtggt
    12001 cagtatgttc tacataccct gtgttgccac tatttccgta ctgtggaacg agtacggatg
    12061 gaaaagggcc atggcagtat gtgtgctgga gatccttttt gcaataattc tcggcggcat
    12121 tgtcagccgg ctgcttaatt ttgttttctg aactgaagcg gcattcattt cagtgtgtac
    12181 cggtagacat tggtttccct gatcctgaaa cccattgtct ggtaaagctt gtttgccgca
    12241 accctggaag gccttgatgt aagttcaatt gtttccgcac ctgaatgttc tgcgaagtca
    12301 agtgcaaatt gcgtcagttc cttcccgtaa cccttgcctc tttccgaatt atcaaccacc
    12361 acatcctcga tccagaaccg ggtaccggca ggtattctgt atattaccag tgtcagcatt
    12421 ccgacgatct tgccggcatt ttcagcagta aacaggtgta ctgatttctg cctgattata
    12481 gccctgaaat gctcccttgt gaggggcctg atgtcaggag aaagctgggg cagtaaacgc
    12541 gaaacagaat tgaataccct gagactgaat ctcttaactt ctttgatttc catggcgtta
    12601 cttgtttgaa agattaaatt atcgtgggtg attctctcct gaaactgcct ggccagggga
    12661 cggtttccgg tagcccagtc cgctgaaaac tgctaccagt gttccgtcct catcaaaaat
    12721 ttccacattg tatccgctta tcctgttctg aacccttatc tccctggcgg ttgccctcag
    12781 tatttttccg gttggtgagc tgaaataggt tatgttcaca ttcagtccca ctgttgtggt
    12841 tccaccggaa ttggaagccg cagcaaagac aaagtcccca agcgtaaaaa tgacaccgcc
    12901 ctgaacctga ttaaggccgt tcaggtgatt ctcgttaagg gccagttcgg ctgcggcata
    12961 tccttccctg acctcgctga gcactattcc ggtgagtctg gcgaacctgt cgttttcaat
    13021 gtttctgata agatcatttt tcatggcggg tatgctaatg aagaatcaag atataaattt
    13081 tatgcataca tcaaattatc cacggtcatg ggatacggat ttatttcatc ctttcactga
    13141 agataaaaaa ccggggagct tttttagctc cccggcactg tgagggatac tgataccgtc
    13201 cggtgaaaat gatctttgcg gcaacagccg cattaatctg ttcaggctcg gcctggttac
    13261 cggagattca gtctatttga ccacttgaac cactgcatat tttgatattt tccagaattc
    13321 cgaaggattt ttaatttcaa tgtaggcgat tgtattttcg ttttcacgaa tcatctggta
    13381 cgaatcagtg ggatgttccg taatgagttt tgcctttttt gagttaacag ggatggtcct
    13441 ggtttttgtg acatcgatcc tgtcaaatag actgtcagag aaatcctcta tcagcgattc
    13501 tttccttcct aagccaagaa agcctccttc cttcgaaaca aggccctttt ccctgaggtc
    13561 cttataggtt cctgttgcca gatatgcctg gttaagcttg tcagtctggc tgtttatagt
    13621 ttcggttttt tgaacaagtg tatcctgaag tgcgaacatt tgcgtgttaa gctgttcgat
    13681 ctggaaatcc ttttcaacca gggttgcctt cagcccggct atttgcgtct catactgttt
    13741 cattgtggct tcaaggtcag tgatccttgt ctgaagaccg gcaatggtat ttcccgattt
    13801 tttaagctgg gcattcaact gtgctatttt cttcttgttt tcctcaataa gtgtatttat
    13861 gtatttaatg tccgcgagta cctgctcttt tttttccttt gggaattctg aatctgaagc
    13921 ctgtacattg atgatctttt ccttctgttt tatttcattc aggtttctct ctatttcatc
    13981 gaaggtgttc agccagtcgt ttatcatcga atcccgttca ttgagttgtt cggtgaataa
    14041 cagcttctga ttttccataa gggtcagctg actgttatga tcccgcttgt aaaccgaata
    14101 caggatcagg ccaagcccga taataaaaaa aacacttatt aaacccgtga tcagagctcc
    14161 cctggttatt ccctcccttc gggcatttct gagatcattt ttcacaacca ggttttcacc
    14221 ggtgcggtta agatcatttc ctgttttgtt gtctcttgtt tccattttta ttcttaggtt
    14281 ttagttgatt tgataaattc gatctacggg agattggcaa atcataagcc taaatgatct
    14341 ctggttggca atatgcttag aatgaatata ttatcaaatc tgacttgtct gacaacatta
    14401 tcaaatcagg aaaataattc tcattttggg aaaaaactgc cggggtcagg tagaggattc
    14461 cttttcctgt ttcagcattc tgtagatggt tgaaatacca atatcgagct tttttgccgc
    14521 aaggctgata tcgttgttgc atctgcggag tgtgtctttc acgattttca tttcgtgctc
    14581 gcggagggtc aggttttcat ctgtccgggt tgctggtgaa tcgtctgaat ccagattgaa
    14641 atcgtcggcg gtaatctctt cgtttaccga gagggttact gcaagctcca cgactgattt
    14701 caattccctg acatttccgg ggaaagaatg ggccatcagt tttttctgcg cgccagctga
    14761 gagtcgtttt tcagggatat tgttcactga gcagaagtcg cgtatgaaat gccgggccag
    14821 tatcaacaga tcatttcctc tttcacgcaa tggcggcatt tctatttgca ggcccagcag
    14881 cctgtaatac aggtcttcgc ggaaatttcc ctttttaacc tcttccctca ggtttttatt
    14941 tgtggccgca attatcctgc aatccgtttt cacgggttta ttattaccga tccttactat
    15001 ttccttttcc tgcaataccc tcagaagctt tgactgcagg ttgatttcca tctctccgat
    15061 ctcatccagg aacagtgttc ctccatcggc ttcctcgaat ctgccgatcc tcctgaaggc
    15121 agcaccggta aaagctcctt tttcatgacc gaataattca ctttcaataa gctcagaagg
    15181 gattgcaggg acatttacgg ctacgaacgc cttgtccttc ctgttggaat tataatgtat
    15241 tgcctttgca accagttctt tcccggttcc cgtttcccct gagacaatga cggttatatt
    15301 tgtgctgacc gccttttcaa taagttcata aacattccgt atggcagggc tttctcccaa
    15361 tatgccctcc cgatagttat attttttctg gacttccttc ctcaggttgc gtatctggct
    15421 tttaagcccc aggtcatttc ttatattctg aacggtattc agaaggcgtt ccctgatatc
    15481 atccgatttg gtgatataat catacgcgcc cagtttcagc agtgtgacca ccatatcgat
    15541 gtcttcctgt tccgagatca ggattacctg tatatcactg ttttcctgcc tgatcttttt
    15601 caggacctcc agaccagaca aatcgggcag cctgtaatcc agtgtcacta tgtcgggtga
    15661 ttcgcccaga ctgttcagga aatcacgggc attgaaaaaa cttctgactt cgtaatccgg
    15721 gttaagggac agggtatata caagaagctt gttataccat tcattgtctt ccaccacaaa
    15781 aatcctgacc ggtctttcat tcatggagat gatgattagg aatggatctg acggcctgaa
    15841 aaatacaaaa attttgcagg aattacggat tttcagttaa acttctcaat ctgttccttt
    15901 aaaagattcc tgacaatttc gaattcacct aatgcttttg ggatcattgt ttctgcggaa
    15961 tccgggatat cattgttccc ggctgttttt tcaacggctg tcagaatgtt gcacaggtcc
    16021 attgccccga tgtgcctgca tggcgggatc atcttgtggg caaggtctga cacagctctc
    16081 cattgactca gtctggctgc cgcctgcatt tcatccagga gtttttccgt ggataccaga
    16141 aatgattcca gcatctggtt aacaaactgc tcatcacctg cagcaatatg tttcaggttt
    16201 ttaagattta ttttttcgga acggggcctt tcttcacttt ttcctgcaga attgccgcgc
    16261 acttcatccg cagtatagcc gccaatgaca ttgagtaccg cagtcagcag cttttcttcg
    16321 gaaaacggtt tctgcagaaa agcatttatt cctgccttgc ggtatttttc ccagtcatca
    16381 ggaggagcag ccgaaacaat aattataggc atatcggatt ccttaatatc catttcatcc
    16441 ctgatgaagc gtgctgtttt taatccgtcg atacccggca tcctcatatc caggaaaagc
    16501 agatcgtatc tttcttcctt gagtaattca agcgcgtcaa cgccgcttgg ggattctgcg
    16561 tagctgatat tccacctgtc aaagaccttt ttgaaaagaa gtctgttata ttcctcatca
    16621 tcaacgacaa gaaatttcat tccacgaagc ctgccgggga ttgccgggga aggtggcatt
    16681 tcatttttca gctccttttc atcacctgtc agataaggca gcctgcagat gatttttgta
    16741 ccctggtttt ttctgctttt aaactcaatt tttccattgt gcagttccac cagtcttttc
    16801 actattgaca aaccaagacc tgttccaccg tattttcgtg tagtactcat ttccgcctgt
    16861 gtgaaatcct cgaagataac atcgatcttt gattcatcaa ttccgatgcc ggtatccgta
    16921 aattccatca gcagttccaa ttcttcattt tgtttaatga tgctgtcaat tgagaaatga
    16981 acactgccgt ttctggtgaa tttcaccgaa ttgcttacga ggtttatcat aatctgcttc
    17041 agtctgaacg gatcgcccag cagtaccggg ggtgtgacgg gacttattga aaaactgagc
    17101 atggtattat tgttccttgc ctgtttttca aacagtgcat aaacttcatc agttatccgg
    17161 ccgacactga agtgaatctt ttcaagagta agtttattgt tatgcagcct ggagaaatcg
    17221 agtatgtcat cgatgatcct gagaagatgg tcggaggaag atttaatgat cttaagtgaa
    17281 tcagtgatcg tttcgttcag aggatcatgt agcagttgct ccgtaaagcc tgaaattgct
    17341 gttaccgggg tccggatctc atgactcatg ttggccacga agagttccct tgtcctggaa
    17401 agtctttcag cttcatcttt cgacttctcc agtgcaacct ggtaagcata ggcattcctt
    17461 gcgtatctta taatgatata taaaacgaga atggctagca ggcctccgga tattgaaagc
    17521 aatacaagca gccggaaagt tttttccgca atctggtcag cggcatcttc ccgggccctg
    17581 atctgctgaa aaacctggtt ttccattttc gtgataagat catagaattt ccctgttatt
    17641 atactgctgt ttactgccag ctgggattcc tgttccagca gctcattctg tgcggtactt
    17701 gttttcctga caacctcatt gatgtcttca atgatctctt tttcatccgt tgttttctgg
    17761 ggggcacggc taaaaaccct tttcaggata ccccttcgct gatctgcagc agttgcagca
    17821 acattcagct catccgacag ctgcttcaga taatcgatca ccatgttgtc tttatataaa
    17881 gtcagaagct gattccagat atagatgttt tcttcaataa gccggcttat tgtatcagcc
    17941 tgaacgagaa gagaagagtc attcctgcat tcctcacgga aaacgctcat ctttccgtcg
    18001 atattagaaa tgatgtcgta ataggatttg atatcggaag gattttttgt gatggtgtat
    18061 attcttacac tgttacctgc ttttcccagg tccatggaaa tatctcgtat gctctgcagc
    18121 ctgagttcgg gattaaagtc gatgcgaatg gaggagacta tggaagagag attgcgataa
    18181 agcaggaatg aggtgactgt tagcaatata acggagattg tcatcaacaa tccgatcttg
    18241 atctggattg aattcctctt caggtatttt ttaaacttgc cagtcatgca tccaaaaata
    18301 cagaatattt attttcaatg tattgctgca tataaaggct gcaaagtgca ggggcaaatc
    18361 tgtccttgac aacaaaatag tcatggggaa cctgcttgat tttttccctg actgtttttg
    18421 attgtgacag tagaactacc atcggatcac cggttagttc catcatcaat ccaataacct
    18481 gcggggccgt aattccttcc ccgagatgat aatttatgaa gacggcagaa ttattacatt
    18541 caagctcacc tgactttatt ttcctgataa aatcaccggc attggtaaac gagcgcagta
    18601 taagctggta tttccccttc tccaggaaag gattcacact ttgttgaatg gcattggaaa
    18661 gcaggttatt gtaatactca tccccttcga tgaccagcac atttattttt tgtttcatac
    18721 cacagctttt tttcatacca ctgcttccaa aattgtgccg gtttttcccg atcctgcctc
    18781 agaaacatgt aacagattta aaaagaatga ttaagagacg aaaggcagtg tttcaacatt
    18841 gaaaatgctt tttcattatt ctcaaaatga gaaactgaaa gaaaaaattc ctgagatata
    18901 agaattggag atggtatttc ccgggaatta tggttttcct gaaccgagga gattcatttt
    18961 ttccccattt cccttatcct ggtgatacaa ttaatcaggg ccctggtggt gtgatagtta
    19021 cctttccaaa tattcgcttt tgttccctcc tttgccctgg gttccttatc ggtggatcct
    19081 gaataccagt ctccgtgctg ccagtcgatc aggtaatcct ttatgaattt ccactggtcg
    19141 aagaagcgtt ttctgtagtt cagtggatca tccggatata attccgacat aatgagaagg
    19201 gtattaagtg tttctgactg ggcccaccag tttttcgtat ccctgattat cgtaacggat
    19261 ttttcatttt tataaaaata accgacatca tagaccccgg catttgaatt atcccatcca
    19321 taattcatgg aattgtcaac catttttttc cctatctcca gggttttgct gtcagccact
    19381 gaattcaggg cctcggatgc ctccatcagg aggtaggctg tctcaacatc atgcccgaac
    19441 gacatatgat ccagggcata gtgcttaaga tgcacctttt ttgatgaatc ctggtacgaa
    19501 accggagtca ggtctgcatt caggtacagc tgaagagatc ccttgtcatt tacgataacg
    19561 tcacgtatca ggtgaagcat ctcgtcaagt ctttttctga ccaggtcatc gggccataca
    19621 tggtacagtt ctgtcagggc ttcaagtata tgtatcgagc tgttctgatc tttcggaggt
    19681 gttcctcccg ggccaccttc ccttatcgga gtaccatcct ggttaagaaa ctggaaataa
    19741 cctccgtaga cgggatcatg actgcctttc tcgagccagg caaatgcttc ttttgccagt
    19801 tcaagtacac cgggatcccg gctttccctg taatatgcgg ccaatccgta gattgcgtag
    19861 gcattgccat aggcattctt agcaaactgt ttaggatcag cttcttttaa aggggtgcct
    19921 tcgcgtgaaa caagattgaa aaaacctcca tactgcttat cccacatgtg atcccttagg
    19981 aacaggaatc catgccttgc ataatcgaga tagacaggat ctcctgtaaa agctttcatt
    20041 tccgaacagg cccatacatg acggctctgg gtgacaatca ttttattctg acttccggtc
    20101 atttcccact tgtagttgaa attactgaga taccccccgt attccttgtc gatcactctc
    20161 ggataccata cgggcatcat attctttagt aaaagatctt ccatttcagc agccactttt
    20221 ttcatgtcgg ccggctggca atgccccgga ttcttaactc ccgttaaaaa aagaagaatg
    20281 attaagacag gatagaagtt tttggatagc gtgatcatga cagtgagttt acaggtgaac
    20341 aataaaatta gcaaaaatcg atttgattaa tacagtattt tcaaagattt tatcatgtta
    20401 tgatactgat aagtttaaaa tcactttacg ttgaatcctg aaatctcaac aacgggtttt
    20461 tctcctctgg tatctgcatc tttcagcttc atatcaattg ttttttcttc tcccggcatg
    20521 aggaatatgt aattatcact gtaaaatacc gggagaatgc gttcgccgct gctccggccg
    20581 acaatcttca gtcttaccat caaagccgga tgaggtgtcg ggttactgag ggtggttgtt
    20641 aagttccagt tgccgcctga tttttttgtt cgggttgaca tcttcagatg aaccagaggc
    20701 agattgttga gagcccggaa gttcccttca tcttttccgc gccagtagaa attctcggaa
    20761 agcagctgat ctccatctgc cagggttagt ttgatgaagt ggacgtcaga aaggttttcg
    20821 gggaactcaa gcctgaagca tgtgaccgtt gagaattccc tgctggtaat tattgttgcc
    20881 ttgttccatt cgacggagcc atccatgttc attataatgg ccttggccgt caggcttgcc
    20941 ttgttccctc cactgtaatt tacgacctcg atatcgttga aaaccgggtt ccattgaata
    21001 tgaatcggtt cacatgcctt tttgcagcca aagtatgcag ctgtcggttc caggtaataa
    21061 tcatatgtct gccagaccat cgacggccag gctggatggc tcatccacag gagaagcccc
    21121 tgtctgaaca tgcttcttcc ttcgaacata cccctgtaac cgtcgtagtt tatccactgg
    21181 gccagcccgg taaatttcat cccgtcggtt accgggccaa atcccttttc aaccatctgg
    21241 ttgaatgttg aagcattctg tgcgctttca aggcaataat cgtgcatacc ccatagccgg
    21301 ttttgcggcc acaatgcatt ttcaggaaga gtcagtccga ggctttccca ggtcattaca
    21361 tttggcatac ccctttcgct gtggaacttc ccatagccaa aaagaagaaa ataatctctc
    21421 acaggcaaag ctttgtaggg accgcctccg cttacaacac ccgatgatga gttggaaata
    21481 taatgcagcc cgggatggag tctgcggatc atggatttca gggcggtgtc aaggatcacc
    21541 gggggattgc cctcgttgcg tccgcaataa agtccgatcg acggatgatt gcgtatcctt
    21601 ttcaccaggt catttgcatt tctcatgaac atccccggat ctgccggatc aggtccgtct
    21661 gcaggattgg cgagccagaa atcctgccag accattattc cgtgccgatc gcaggcctca
    21721 tagaattcgt catcacctgt ttgtccgacc cagttcctga tcattgtaaa gttcatatcg
    21781 gcatgatatg ccacggctgc gtcgtattcc ctgccacgat accggagatt ggattcggga
    21841 aagccccagt tgccgccacg gccggtaaaa cgcctgccat tgatgtacat gttgagggtc
    21901 tgatagtttt cggtgaaata catttccctg ataccggatt tgaaactggt gtgatccgaa
    21961 aactccccgt tttcagtctt aaaggacagt tccacatcat acaggtgagg attgccatat
    22021 cccctgggcc accatagcct tggattttca attcttaata cgggatgcgt ggaagggttg
    22081 agttttataa gttttgtttc cgatccggca aggacaacat ctgtttcaaa tgaaacatct
    22141 ccgtactttc ctgacaaaat gcccttaatt ctctccctgc tatggttttt caacgtaact
    22201 tccacaaaaa gatcggcact ggacgtatca ggaagcggga gatccggaag gataaacgga
    22261 tcaattattg agacatctcc ggtagtgctc aggaaaacat cgttccagat tccggtgttc
    22321 ctgcccctta ttgtcgggat ccagtcccag ccaatggagg catggtaggt aggattgtca
    22381 gcacccagtg aacccccgtt tttgtcggcg ctaagggcag tcttttcctt cacagtacca
    22441 atattttcat tcttccggat aagcacagcc agggcattta catggtcggg tacgatcagt
    22501 gatgttatgt caaatctgcc gcgaataaat gctccatcga tttttcctgc ttctttcccg
    22561 ttgacaaaaa catctgcttt ccagtttatc ccgtcgaaat tcaggaataa acgtttccca
    22621 cggaaatgct caggaagatg aaattcattc ctgtaccaga agtcagaatt gaaaaaggat
    22681 tctgaaatca gaagctgatt gtctccgaag tcaggatcag ctactgctcc ggcattaatg
    22741 tagcttacaa ggattgttgc aggtacggtt gcatcaaccc aatccttgca gtcaaatccc
    22801 tccgatgaaa tgatctgacc ggttgccgat acttcagatg ctctctgtac cttccagttt
    22861 ccaccattca ggaagaggcg cccatgtccg gcaggggatg gggctttcgg tctcgttgcg
    22921 agtcctccag taccaaagac ttccagttca cttaacacga acttattgcc ggactcaggt
    22981 ttttccatta gtatccttac gaagcgcccc ctgccatctt tcacaggcag gtcatcaatc
    23041 cggccgtcat gacccggcag gggcgcaaca tccttccatc tttcagaatc atccgaaacc
    23101 tgaacgcttc cgccgagggc tttgttaatc caatgcagtc ttatgcggtc aaatgaactg
    23161 gcggaaccca ggtcaacgta tatccattcc cgctcgttac cggcgctcat ccaggcactt
    23221 ttaaaattat gggaaggtgt aacctcaagc ctggatcctc cgagataaaa atcgagatcg
    23281 cttattgtcc atttctctgc acagggtgat gtaaggatta ttctgaaata atgccaggca
    23341 acgggaccca ctggtgtaaa caccgggtta atgagccgtt gcattggttc ggatcccgat
    23401 ccggagctgg tctggaatga gggtctggga gctattgaat ccggcccgta aggacttggc
    23461 gggaatgtgc cggagcgttt gacggagatg tttctctttt tctggccctg ctggttgatc
    23521 attgcagcag atggatccct tttttcttca ccaggtaaac ctgctcctct gatctcagag
    23581 atttcttccc agtttattcc gtcgtcggag ccaagacaga caaatttcca tccgcaggtt
    23641 ttcttttcat tatatatcat tgagccattc aggcatatcc ggtctgcaat gatcggtttt
    23701 gcttccctgt tgatactgaa ttcaatccat acctctgatc cttcaaggtt cacttctgta
    23761 gcactgttgt gatcaaggag ccattccctt tcatttatcg gggcgacacc gctgcaggtt
    23821 gaaacggaga tgtgatctgg tatttcatgg gtgaaaattc cgtcggttac aagctgtgcc
    23881 gtcagattat agtcatagct tgaggagtgg aaagcaggcc tcagcctggc caggttccgg
    23941 taagtggttc tgtcaggtgc caggtcaggg gaaaaatctt cccgcagatc tccagggtat
    24001 attccagtac cgcggttgta gcggatattt tttttgtgac aaccgggaac tgaaatggca
    24061 aaaaacaata accccaacag aaaacaacct gcttttgtcg acttcatact gattatactg
    24121 aatttgaatc atccgggcca cgacattgca cctgtgagcc ggccagggtc tgattaccgg
    24181 gcgttaactg gtaaatccca ggttttaact tttacgccta gattcggtca aaaacaaaag
    24241 tttcttcagt ttctccccag aaaacggctc tgacataatc aatgacaagg ttgtccccgt
    24301 caaatttaaa aatctctgtg ataattgtct cattgccgtc ttcctggacg attttgctgg
    24361 aaaccttcaa tgattttccg tcttcagacc attttgccgt cgatgtttta accgattcca
    24421 tgaatgcctt gttttggcat tccttaccat ccagggtata cttgtcggtc atcactgaag
    24481 tttcgccgcg aaaatcctga tgacgctcaa tggtcatatc attgccatct tgtgtaataa
    24541 caacctgaaa tggtgcagaa ctgaaatctt cattcagttt gcttttcccg gtgtttagct
    24601 tccatttgcc tgaaaagtcg gtttcctggg ctgaggcagc cagtgaaaca gtcaggagca
    24661 gaataagaaa aacagcattt ttcattttga tatagttttt attggactgt tctgagggag
    24721 cccggatgtt gaaaaaaata ttattacgtg tttatgtatg tcaggtatgc gagtttcatt
    24781 taaatattaa acaacttatg caattggctt gatttgtcaa tgcaggaaat tcttatttaa
    24841 tcggtttccc attacttgat aaaacccgga gtcaggtggt aaatgcatga tttttcaggg
    24901 ataagggaga taaacaggta tcagtcggtc agccgagcag ctgttttact ttcgctgaga
    24961 tctctttgcc gtcggccctg cctgccagtt ccctgactgc ggctcccatg acttttccca
    25021 tttcagccgg ggatgttgca ccaagacgtg atataaccat cttaatgatt tccgtaagtt
    25081 cctcatcgct taattttgcg gggagatatt tttccagcac atctgcctcg gcggtctcct
    25141 tttcgtaaag atcggtccgg ttctgttccc tgtagatggc agctgattcc cgcctctgtt
    25201 tgacaagccg tcttacaatg ttcagttcat cttcggtaga aagaacggca tcagttcctt
    25261 tttcggattt tgcaagcaaa aaagcggttt tggctgccct gatggcttca agtgccacct
    25321 tatccttacc tttcatggca tttataagat cgctggctat cttttccgca attgacatgg
    25381 tacaaatgtt tcagatagtt tctatttgca acttaaatat aaaagtaata gaaataaaag
    25441 aaaagacaaa gacaaaaact gatttcagcc tgaatgccaa tgcagccggt aacggaacat
    25501 ggttaaaaaa ggcaatttat gggaatatat ccctgtagtt gccctggcaa acaaatttta
    25561 agaactttaa ccggagttca gttcaagagc ccagactgca gccggatatt gccaatcagg
    25621 aattgtttgg tgatcattcc cggttaataa tgattgtccg tgttattctg gttatattat
    25681 ttccgatact taagaggtac attccatttt tcgtgatcgg attggaaatc gtaagtattt
    25741 cacctgaaca atgtactttt tgctggtaca ggattgtacc ttttaaacta taaattacga
    25801 ggttcatttc gccaataaac tctctgccca gatcgacata aaattcattt ccgcgtacag
    25861 ggaccggata aattacaatg gagcctaatt gttcctttgt caacgggaaa taatcggcta
    25921 atttaataat aaaaggcaaa ctagggtcgg aggtattccc ggattcatca actgctacta
    25981 tggttatttc atattttatt gaatcagaca gattgacgaa tagaaattct gattccttca
    26041 aagtgataac cggcatgtcg ttgcaatata tttcataacc cgatactccg acattatctg
    26101 tagaaggtgt ccagtacagg ctgaagcctg aattttggag attttttacc gacaggtttt
    26161 caggagctgt gggtggatgc aggtcgggag ttttaacttt cagtatctgg ctaaaagccg
    26221 aaacattacc cgctgcatca aatgccctta ccgtaaggtt ataatccgta aaaacggtta
    26281 gtcctgaaat atccgctttt gaagttgttg tagttgccaa caggatatca ttgcaataaa
    26341 cctcgtactt ggaaactcca acattatctg tcgaagggct ccagttaagt gtaaggctgg
    26401 tttctgaaag attgctggct tttaaattag ccggaacact gggagggtgc gtatcatttg
    26461 tattgaccct caaagcatca cttaaaggag agatattgcc tgatgcatcc ttggccctta
    26521 cggtcatgga ataggtggat gccggtgaca aaccgctaat gattgccgac gtacctgtta
    26581 cgcttgtgaa cagatttccg tttcggtaaa tatcatatcc tgctacggac gaattatcag
    26641 ttgaggctga ccatgaaagg gtaaaactgg tttcggatat tttggttgca accagattgg
    26701 ttgggacagt cggggcatga gtatcaggtg ttcttacgac caatgcatta ctgtgattgg
    26761 aaatattccc tgcagcatct ttcgcccgga ttgtcatact gtaatttgtt aggagcgtaa
    26821 gaccggtaaa actcgctgtt attccgttaa cagaagtact caatacgccg ttgcaataga
    26881 tatcatatcc ggttacaccc acattatctg aagaagctga ccaggacaga gtaaaacttg
    26941 tttccgtgac acccgttgca acaagacctg caggtatact ggggggatgg gtgtcgattg
    27001 ttgtaacact tagtggggaa ctggcggcag atctgttttc agcagcatca acagccctga
    27061 ctgtcattga atatgttctt gaaggtaaca gtcctgtaat attagctgat gtgccggtaa
    27121 ccgtcgcagc caggctcccg ttgcgataaa cttcatagct ggttactcct acgttgtcag
    27181 aagaagccgt ccatgaaaga ataaacccgg tttctgttat attgccggag acaaggt
//
LOCUS       DAOOYG010000025        25849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1687_length_25849_cov_6.259091, whole genome shotgun sequence.
ACCESSION   DAOOYG010000025 DAOOYG010000000
VERSION     DAOOYG010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25849
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1687_length_25849_cov_6.259091"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PK288_03925"
     CDS             complement(<1..1100)
                     /locus_tag="PK288_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPM18101.1"
                     /translation="MMNNHFADVILPLALRGTFTYSIGNEFTGKVFPGMRVLVQVGSK
                     KVYTGIICRIHDEPPALEKIRPITGILDDVPVADAIQIRLWQWISGYYMCTPGEVMKA
                     ALPSALFPEGMVAGTLREKYRPRKEAFVQIHTESAGDELNLIIDKLNRSPKQRHLFIT
                     YLKMSGYPGCSEKIPLKKELLLREADVSQAVLESLVRKKILVSLMLETSRLSNQENRR
                     DPFSILSDPQKKALDKIRHLFSEKGIVLLHGVTSSGKTEIYIHLIEEQLKQGRQVLYL
                     LPEIALTTQIINRLKKHFGDSTGVYHSRFSEAEKVEIRNKVASGDAGGGYGLVIGVRS
                     ALFLPFRKLGLVIIDEEQDPSYKQHDPAPRYHA"
     gene            complement(1128..3011)
                     /locus_tag="PK288_03930"
     CDS             complement(1128..3011)
                     /locus_tag="PK288_03930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM18102.1"
                     /translation="MNWYKLNNTSFRFIFFSASLFFSLLFPYRLSAAPADQVPIVSGR
                     ALEVALVPLPAYIISLILLLAAACILIIIFSTRLRNTNRELTARNKQVEDINQKLQKA
                     NNELLSQKDALEREYSNSEMFYRMLIRSADDGISFYDRNWKLKFTNPAFYSVIGYTQQ
                     EYESLKMSELVHPDDSDYEKRRIAGLSSRDFFESELRLRHKDGHYVNLSTRSVAVKND
                     EGETIGSLTVSRDITSLKKVHEELMRANAEAEASNRLKSSFLANISHEVRTPLNSVVG
                     FANLLLSSDLSNEVKEEYIEHINHNSEKLLQIIGDIIDLSRLESAQIEITYEEASINS
                     IVNEVVDEARKIVKRNEKSIIIIVKNLLEIENDLVFTDRVWLKRVLNHLLDNAVKFTL
                     EGSIQITCSKESDNLVFRIRDTGIGINKENLDHIFEEFRQEINGHHRPFEGLGVGLTL
                     AKEVIERMGGKIFVQSEKGVGSEFSFSLPYRPAGGSSRMKSKIMSQDNLGSQNNWSGK
                     KCLIVDDNKDILLYLERVLSETGITIFSARSGFEAIDILKRTTDIDLILLDMQMPEMN
                     GIEATRELRKIRGDIPIIAQTAFIFEDDKDIILEAGCDACLIKPIRKEHLLTVMSGFI
                     KSA"
     gene            3164..3679
                     /locus_tag="PK288_03935"
     CDS             3164..3679
                     /locus_tag="PK288_03935"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HPM18103.1"
                     /translation="MERPRNHFTRGRSYKNDEITVYWKPDACIHASYCYRELIEVFDP
                     GRRPWIDLSGSNTERIIEVVNMCPTEALSWKWNEDARNIDVERDQTNHIKFKRPELMS
                     TEKAKDEIRPVSVKIMVDGPVVVKGNFTIRYNGKEKNFTNGIISLCRCGGSNQMPFCD
                     GTHRKIGFEDK"
     gene            3685..3891
                     /locus_tag="PK288_03940"
     CDS             3685..3891
                     /locus_tag="PK288_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HPM18104.1"
                     /translation="MERNELDENTGTSAIVEIIDNGPIKISGEIMFWDLKRDITDSPS
                     EIYLCRCGKSSNKPYCDSSHKMRL"
     gene            complement(3905..5110)
                     /gene="sucC"
                     /locus_tag="PK288_03945"
     CDS             complement(3905..5110)
                     /gene="sucC"
                     /locus_tag="PK288_03945"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPM18105.1"
                     /translation="MNIHEFQGKSLLSAYGVEIQEGFVAETASQAVLVAKQLQEKYGS
                     RLFVVKAQVHAGGRGKGGGIKLARSPEEAGEIAEKMIGMTLVTPQTGPAGKKVNRVLV
                     ARDVYYKGESEPEEFYVSILLNRNKGRYVIMYSPEGGMDIEQVAEQHPEKIFTEEISP
                     STGLLDFQARNISFNLGLDGTASRNMHKFLKGIYETFTGCDAQLLEINPVLKTSDGKI
                     LAVDCKFILDDNALFRHNDLVGMRDLREEDPIEVEAGRYNLNYVKLDGNVGCMVNGAG
                     LAMATMDIIKLSGGAPANFLDVGGGASASTVEAGFRIILKDPAVKAVLINIFGGIVRC
                     DRVANGVVEAFKSVGKIRVPVVVRLQGTNAQEAREIINNSGLSVIAATSFGEAAEAVG
                     KVLRSVQGV"
     gene            5214..5753
                     /locus_tag="PK288_03950"
     CDS             5214..5753
                     /locus_tag="PK288_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPM18106.1"
                     /translation="MRKLQNSELGRISTEQFRKSEKSPFVIVLDNVRSHNNVGSVFRT
                     ADAFRVEKIFLCGITAKPPHRDIRKTALGATESVEWHYHESTIDAISELKAGGYTVVA
                     VEQAEGSVELQDFTTKSNRRYALIFGHEVNGVDQKVLDLCDACIEIPQFGTKHSFNIT
                     VSAGIVLWELNRKISNLVK"
     gene            complement(5841..7553)
                     /locus_tag="PK288_03955"
     CDS             complement(5841..7553)
                     /locus_tag="PK288_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM18107.1"
                     /translation="MKKIKSYFILILAAVVFSGCSGLNKMKKNAGDIKYEVTPKVLEA
                     HGGMVNVTIKGTFPQKYFSKKALLEAAPVLTYAGGETAFDKVQVLQGEKVQANNKVIT
                     YNGGDFTYTSSIPYKDAMKMSELILRAKASQGNKSLDFDPVKLADGVIATSTLVNVHA
                     RPILMPDRWQRIVPESQTADILYVINQADIRNSELRKESIKELKNYITQVTVDPNRQL
                     KNLVVSSYASPDGPFDLNENLSVRRGTSADRYVKREFRKIEAAKADDFFQSLTTPEDW
                     EGFKSEVEKSSLADKDLILRVLSMYSDPVVREREIKNMSAAYEALKTDILPKLRRSKM
                     TANVDKIGRSDEQILAQIKSDPKALGLEEMLYSATLTNDPNEKLQFYRITAENFPKCI
                     RAHNNVGYVLLGLGKADEALAAFEKARAIQNNDIVKNNIGFVYLVQGDMAKAEEEFNS
                     MTASTPESRWGLGVIAITKGEYDQAVNFFGNEACYNHALALYLKGDVNRAKTMLDGLT
                     ETCMGKVPYLKAIIGSRLDDKAYMLNGLREAINLNSDLKAYARTDYEFAKFFADDAFK
                     GLVQ"
     gene            complement(7678..8319)
                     /locus_tag="PK288_03960"
     CDS             complement(7678..8319)
                     /locus_tag="PK288_03960"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPM18108.1"
                     /translation="MKVKYNYLVIEGNIGAGKTSLAEMIAKKYNARLILEAFADNPFL
                     PRFYADPDKYSFPLELSFLASRYKQLNEELGLADLFKSFTVADYYFMKSLVFAAITLK
                     GDEFNLYRQIFYIIYKSLPKPDIYVYLHIRPERLLANIAGRGRSYEKSITREYLQRIQ
                     DSYFTYFRQNPENRYLVIDASNLDFVNREDHFLRITDLIFGKEFSSGLNVVIL"
     gene            complement(8485..10647)
                     /gene="pnp"
                     /locus_tag="PK288_03965"
     CDS             complement(8485..10647)
                     /gene="pnp"
                     /locus_tag="PK288_03965"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPM18109.1"
                     /translation="MFNIKEKNIDLGDGRIITLETGRLARQADGSVLLKFGKTMLLAT
                     VVAAKEAKEDTDFMPLSVEYREKYASSGRIPGGFLKREARPSDYEILISRLVDRALRP
                     LFPDDFHAETFVTVNLVSADLDIIPDALAGLAASAALTVSDIPFNGPMAEVRVARING
                     QFVVNPTASQLKDSDIDLMVGATYENILMVEGEMREVSESDMLEALRVAHSAIKILCR
                     AQVEFAEELGATTKREYCHEVNDPELRKRVWDETYDKCYQVARSLTADKHRREEAFES
                     VIAEFLAQFPEEEEINETLVRKYYHEVHKEAARRLVLDENLRLDGRKPDEIRPIECEI
                     DPLPATHGSALFTRGETQSLTTVTLGTKLDEKIIDDVLNQGTEKFTLHYNFPPFSTGE
                     ARPIRGISRREIGHGNLAHRALKNMMPPEEENPYAVRIVSDILESNGSSSMATVCAGT
                     LALMDSGIRLRKPVSGIAMGLITDKDSDKIAILSDILGDEDHLGDMDFKVAGTRDGIT
                     ATQMDIKVEGLSFEILAKALDQAKKGRLHILNIMNRTISEPRPELKPHAPRIEMLTIP
                     KEMIGALIGPGGKVIQEIQRETGTTIIVEEINGHGDVNIFGENKDAIVAALEWVRKIV
                     AVPEVGQVYRGKVKTIVQFGAFVEILPNKDGLLHISEIDWKKIKTVDEVLKEGDEVEV
                     KLIDIDPKTGKLKLSRKVLLPRPPKEEGGHKRPEDKRH"
     gene            complement(10729..10998)
                     /gene="rpsO"
                     /locus_tag="PK288_03970"
     CDS             complement(10729..10998)
                     /gene="rpsO"
                     /locus_tag="PK288_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPM18110.1"
                     /translation="MYLTTDKKQEFFKTFGSSEIDTGTAEGQIALFSYRISHLTEHLK
                     KNKKDFSTQRALIKLVGRRRRLLDYLKVKDIERYREIVKALKLRK"
     gene            complement(11300..12622)
                     /locus_tag="PK288_03975"
     CDS             complement(11300..12622)
                     /locus_tag="PK288_03975"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPM18111.1"
                     /translation="MKEEFLHYLWKYALYDKDALVDHEKNRIIVIYPGDYNRDSGPDF
                     FNARINIGGTEWAGNVEIHTRSSHFNAHGHNLDHAFDNVILHVVGENDKKVFNARGNE
                     LLTVEIKYDAALYQRYIDLVNNPAVVACQGYVNRMDRFIIRHWLHYLLAERLQQKSAA
                     IFRTFRDTGNDWDETFYRILTRYFGFRVNTDPFEMLATALPFKIIRKHADNRLQVEAL
                     LFGTAGMLEEGLFKNAIADDYYSQLVREFRILSAKYSIVPIHGWLWKFSRLRPVNFPT
                     VRISQLAAMLSVAGGLFSRVLEAEEICRLRELFEVTSSPYWDDHYIFGRKSKSVPKSV
                     GLQAAELLLVNAVIPLIFVYGQYSGKEGIWGKALDFLDELHAEDNSVIADWRKSGIEA
                     GSAFDTQALIHLRDEYCKKRRCLECRIGAVIISAGKDLKKQEELLLEP"
     gene            complement(12805..13632)
                     /gene="pyrF"
                     /locus_tag="PK288_03980"
     CDS             complement(12805..13632)
                     /gene="pyrF"
                     /locus_tag="PK288_03980"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPM18112.1"
                     /translation="MDHNGLFRNIVAKKSFLCVGLDPEIEKIPEFLHRYDDPVFEFNR
                     RIIDATAGYAVAFKPNLAFYETLGYRGLISLESTISYIRENYPGIFIIADAKRGDIGN
                     TSRMYAKAFFEKMQCDAVTVAPYMGEDSVVPFLSYQGKWVVLLALTSNKGADDFQYHY
                     GDGMRLFEKVIRTSRNWGTIDNLMYVAGATRAEMLAGIREIVPDHFLLVPGVGAQGGS
                     LREVARHGMNDRCGLLVNSSRGIIFADNSDKFDNVAGRKAMELQQEMAEYLAEAGLI"
     gene            complement(13639..14724)
                     /gene="prfA"
                     /locus_tag="PK288_03985"
     CDS             complement(13639..14724)
                     /gene="prfA"
                     /locus_tag="PK288_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPM18113.1"
                     /translation="MANNLILEKLEGVKARFLEVGELLTHQDVLSDMERYIRLNREYK
                     DLQPIIAAYENYRLTLANIASTKELLASEKDEEMREMAKAELEELNDRVPGMEEEIKL
                     LLIPADPEDEKNAVMEIRAGTGGDEASIFAGDLFRMYSKFFEQKGWRADINSYSLGTA
                     GGYKEIVISVSGEGVYGTLKYESGVHRVQRVPQTETQGRVHTSAASVVVLPEAGEFDI
                     EIKNEDIRKDTYCSSGPGGQSVNTTYSAIRLTHIPTGIVVTCQDEKSQIKNYDKALKE
                     LRTRLYNLEYQKYLDDVSRKRKTMVSTGDRSAKIRTYNYPQGRMTDHRINLTLYNLPS
                     ILDGNIGEIIDKLQMAENAERLKENNI"
     gene            complement(14934..16103)
                     /locus_tag="PK288_03990"
     CDS             complement(14934..16103)
                     /locus_tag="PK288_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPM18114.1"
                     /translation="MSEESRYSKRGVSSSKEDVHKAINGIDKGLYPGAFCKIVPDLLG
                     GEPDFCNIMHADGAGTKSSLAYVYWRETGDMSVWKGIAQDAVVMNTDDLLCVGAVNNI
                     LVSTTIGRNKRLIPGEIISALINGTEEVLEDLRSLGIDIRSTGGETADLGDIVRTIVV
                     DSTVVARVRRSEVISNHNIRPGDLIVGLASSGQTTYEKEYNSGIGSNGLTSARHDVFA
                     HYLAEKYPESYDTDLPSDIVYSGSLALTDIPEGFSLDYGKLVLSPTRTYAPVIKAILE
                     AFRERIHGMIHCSGGGQTKILNFIDNLHIVKDSMFSIPPLFRIIQEQSGTPWSEMYRV
                     FNMGHRFEIYLCEEDASQVIDIAKGFDLDARVIGHCEKSDQKRLTIVSEPGKFEY"
     gene            complement(16325..18307)
                     /locus_tag="PK288_03995"
     CDS             complement(16325..18307)
                     /locus_tag="PK288_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM18115.1"
                     /translation="MRRVLLFIILLTFMASPDGSAQKRKTERAYASYNAGEYYEAIDH
                     FKDAYSKSSKTDRAARTELGYMIAECYRLTNDPKNAETWYRLAAKSSFSKPEASFWLA
                     STMKKNGKYQQAIEEFRKYKQIAPTDIKADQEIRACELALEWQRNPEPYKVEELKDLN
                     SRKSDFSPAYGRDDYSLVYFTSSRDDAAGNKTHGATGQNFTDIFESRLDKKSKWSTPV
                     PVTDLNSEFEDGTPCFSSDFSEVYFTRCEAGKREKKGCIIMYSKRNGDRWSKPQDIKI
                     LPDSLVAAHPALSADGHILYFVSEMEGGFGQKDIYYTTKSGDNSWAPPRNLGPDINTS
                     GNELFPYVREDGILYFASDGRIGMGGLDIFKAVPQPDGSWVVTNMKSPINSPADDFGI
                     CFEKGSERGIFSSTRKGRGNDDLFSFELPPLQFSVTGLVKDEKTGSPIAGSVIQLIAS
                     DGSNLQAETGTGGEFKFSLKQDVDYIFLASKKGYLNGKEKETTKGQEKSRDFMVTILL
                     TAIDRPIELPNIFYDFGKWDLRPESMVSLDKLVETLNDNPNVTIELMSHTDSRDTEEY
                     NLELSQKRAQSVVQYLIEKGIAPERLFPKGYGESTPKVVDDAIAAQNSFLKPGTVLTE
                     QLINSLGSEEQKEIAHQINRRTEFRVLRTDYEPSGK"
     gene            complement(18562..20751)
                     /locus_tag="PK288_04000"
     CDS             complement(18562..20751)
                     /locus_tag="PK288_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPM18116.1"
                     /translation="MAELRFSALREVFNREPVTVRTPEKNVSKYFGINVFDLHKMEHY
                     LSAEAFRVVKQAIELGKSLTREEANQVAIGLKAWALERGATHYTHWFHPLTEGTAEKH
                     DGFIEMGERGHIVENFSGEVLVQSEPDASSFPSGGIRNTFEARGYTAWDVSSPVFIVG
                     ATLCIPTIFVSYTGEALDYKAPLLKALSALDKAATAVCRYFDRDVTKVIATLGCEQEY
                     FLVDEALYYARPDLVLADRTLMGHQSAKDQQLSDHYFGSIPERIMSFIEDFECEAYKL
                     GIPIKTRHNEVAPNQFECAPIYEEVNLAVDHNQLLMDVMKRIARKHKFRVLFHEKPFA
                     GINGSGKHNNWSMQTNTGKNLLSPGKNPKSNLQFLTFLINVIKSVNDSNDLIRASVMS
                     ENNSYRLGGHEAPPTIISVFLGSYLTGMLDDIVNKVTDRKMTPAQKTELKLGIGKIPE
                     ILLDNTDRNRTSPFAFSGNRFEFRTVGSSANCSAAMIALNAAVAYQLVRFKKEVDLLI
                     RKGRNKDEAIFQILKKYIQESQRVLFEGDNYSRDWHDEAARRGLSGIASVPDSLKAYL
                     AGNSREVLISTGIFSEKELEGRVEVEFEKFTKKVQIEARVLGDLAINHIVPTGIRYMT
                     SLIENVRGLKEIFDEAEFSKLAAESKDLIIRISDHISAIKKLENEMVEARKKANIITD
                     SYIKAIEYENNVKPFLDKIRYHIDKLELIVDNEIWPLPKYRELLFTR"
     gene            21050..23167
                     /locus_tag="PK288_04005"
     CDS             21050..23167
                     /locus_tag="PK288_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPM18117.1"
                     /translation="MSALQFLREKAGVLVAVVIGISLFIFVVSDFFGGGRGQRLRAKK
                     YYEIGKIGNETVSYQDYEQKVQNLLDIYKLSGNTSPDANTVESIREQVWQQMIREELL
                     DKQYVKLGIGVSSEEVDELVLGNNPHPIVTQLFTDQETGLFNKSFLVNFLKQTELDET
                     AKRYWLYFEDEIVNDRMSSKYNTLVSKGLYVSSRQAEFDKNLTAATVDFSYIMKNYSS
                     VPDSLVTVSRGEIESYYSRHKENYKRTALRDIEYVTFDIVPSEEDIKQAEEWINKTKV
                     EFAEATDPEQFLNLTADTRHVGFYEPISQIPENLVEFVKKENTSEVFGPYEENGTLKL
                     ARILNIANRPDSVHVRHILISPNSSGSLDQARSKADSLVKLIRSGTSFSFLAMSNSDD
                     RGSSQLGGDLGWFPEGRMVVPFNNACFSGKKGDILTVETSFGIHIIEILEQSKNIRKY
                     NIGIIDRKIIPGPVTNQRIYSEASQFAGTNNTYEKFNQAIATEGLTKRVANDVTPMQK
                     TLPDLENPRSLIISLFQTERGHIVLDNNQQAVFEIGEKYVVAFCTKVQEEGIAPVKDV
                     ENEIRFVLIKDKKAEILSAEYKKNMQEGKSLDELASDMKLTVREATQVNFRSYSIPDA
                     GSEPALIAAASAAKQGVLSGPVKGENGIYMLTVNEISNSDSDDIRSLKDRLSITYQMR
                     GSYEAYEALRKATNIVDKRYKFY"
     gene            complement(23190..24977)
                     /locus_tag="PK288_04010"
     CDS             complement(23190..24977)
                     /locus_tag="PK288_04010"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPM18118.1"
                     /translation="MKLISDDEIKNPDRIYIPEGPVIEETLLRILSHKQLSKLDRKLY
                     GKDNIFLINSLLEELELKFDIPDEDLENIQPEGSFITISNHPYRGIDTLLLYKLIYPK
                     RNDFKIVRNYLLPGIESFDSSNLSMTDSFNPAGINSLFTDFNEAPIHIESRHCLGIFP
                     AGASAAQFEDSHVILDREWQQSAIKFIKNANVPVIPVYFHGTQIRLQYIFRKINPLMK
                     LTDLPSEILNKKNRIVRIRIGAPITVREQEEFKDISQYGRYLRARTYLLGSKLEVKRF
                     FNLKPAVRKVNPEPVAAPLSGQLLREEYERIKSEYELFSTKNYSVVCAPTRVIPHLIN
                     EIGRLRELTFREVGEGTNKSTDIDEYDLYYNHLFIWDNDADRIVGSYRIGKGREIANI
                     YGIRGFYINSLFKIRNSFLPVLKESLELGRSFIVKDYQKKAIPLFLLWKGIMVFLLRN
                     QEYRYLIGPVSISNDFTKLSKNLIVGFVKAYFYDEKMGRAIIPKKKYVVRYDRIVDQK
                     IFIDVADHDINKIERIIMNIEPGYRIPVLLRKYLEINGRIIGFNIDPKFNDCLDGLLI
                     LDLYKVPPDYIKGLAKEMDDDQIIERFRL"
     gene            complement(25324..25433)
                     /gene="rrf"
                     /locus_tag="PK288_04015"
     rRNA            complement(25324..25433)
                     /gene="rrf"
                     /locus_tag="PK288_04015"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(25551..>25849)
                     /locus_tag="PK288_04020"
     rRNA            complement(25551..>25849)
                     /locus_tag="PK288_04020"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         6986 a         6087 c         5271 g         7505 t
ORIGIN      
        1 gcatggtatc tcggtgcagg atcatgctgc ttgtacgacg gatcctgttc ttcatcaatg
       61 attaccaggc caagttttct gaaaggcaga aaaagggcag accttacgcc gatgacaagg
      121 ccgtatcctc caccggcgtc gcctgaagcg accttgtttc ttatttcaac tttttcagcc
      181 tcactgaacc ttgaatgata aaccccggtt gaatctccga aatgtttttt taacctgttg
      241 atgatctggg ttgtcagggc tatctcagga agcaggtaaa gcacctgcct gccctgtttc
      301 agctgttctt ctataagatg aatatatatt tctgttttcc cgcttgaagt gacaccgtga
      361 agaagaacaa tacccttctc tgaaaaaaga tgtctgatct tatccagggc ctttttctgc
      421 ggatcgctca ggatactgaa gggatctctc cggttttcct gattgcttag acgactggtt
      481 tccagcatta atgaaaccag gatctttttt cttacaagtg attccagaac tgcctgagaa
      541 acatctgctt cccggagaag caattccttc tttagcggta ttttctctga acaacccggg
      601 taaccgctca tcttcagata ggtaataaac agatgccgct gcttcggcga cctgttcagc
      661 ttgtcaatta tcaggttcag ttcatccccg gcagattcag tgtgaatctg aacaaatgct
      721 tccttccttg gccggtattt ttcgcgcaat gttccggcaa ccatcccctc gggaaaaaga
      781 gcagagggaa gtgccgcttt cataacctcc cccggagtac acatataata tcccgatatc
      841 cactgccata atctgatctg gattgcatcg gcaaccggga catcatctag tattcctgtt
      901 attggtctta ttttttccag cgcaggcggc tcatcatgta tcctgcatat aatgccggta
      961 taaacttttt tgcttccaac ctggaccaga accctcattc cgggaaaaac cttcccggta
     1021 aactcattac caatgctgta ggtaaatgtt cccctgaggg caagaggcaa tataacatca
     1081 gcaaaatgat tattcatcat cagcggcaca tggggtatct ctgattatca ggcggatttt
     1141 atgaaccctg acataaccgt aagcagatgc tcctttctta tgggctttat caggcatgca
     1201 tcgcatcccg cttccaggat gatatcctta tcatcttcaa atataaaagc tgtctgggct
     1261 atgattggaa tatcacccct gatttttctt aattccctgg tggcttcaat gccattcatt
     1321 tcaggcatct gcatatcgag gaggatcagg tcaatgtctg ttgtacgttt caggatatca
     1381 attgcctcga aacccgatcg tgcggaaaat attgtaattc cggtttccga gagaactcgt
     1441 tcaagataaa gaagaatgtc cttgttgtca tcaacaatca gacacttctt gccactccag
     1501 ttattctgac tgcccagatt atcctgtgac attatcttgg atttcatcct tgagcttccg
     1561 cctgccggcc tgtaaggcag agagaaactg aattctgatc cgactccctt ttctgactgc
     1621 acgaaaattt taccgcccat tctttcaatt acctcctttg caagagtcag tcctacacca
     1681 agtccttcaa aaggcctgtg atggccattt atctcctgcc tgaattcttc aaagatatgg
     1741 tcgagatttt ccttgtttat cccaataccc gtatccctga tccggaaaac caggttatca
     1801 ctttccttgc tgcaggttat ctggatggat ccttccaggg tgaatttcac tgcattgtcc
     1861 aacaaatgat tcaaaacacg cttaagccac accctgtctg taaagaccag gtcattttca
     1921 atttcaagaa ggttcttgac tatgataata attgactttt catttctttt tacaatctta
     1981 cgggcttcat ccacaacctc gtttacaatg gaattaattg aggcttcttc gtaggtgatc
     2041 tctatctgtg cgctctcaag cctggaaaga tcaataatat cgccgataat ctggagcagc
     2101 ttttcactgt tatgattaat atgctcaata tattcttcct taacttcatt ggacagatca
     2161 ctggacaaaa gcagattggc gaaacccacc acactgttca ggggtgttct cacttcatgg
     2221 gaaatattgg caagaaagct ggatttcagg cgattgcttg cttcagcctc cgcatttgcc
     2281 ctcataagtt cctcgtgaac tttcttcaat gaagtaatgt cacgcgaaac agtaagagaa
     2341 ccaatggttt ctccttcatc attctttacc gccactgaac gcgttgacag attcacataa
     2401 tgcccgtcct tatgccggag ccgaagttca gattcgaaaa aatcacgtga actcagaccc
     2461 gctattctcc tcttttcata atcactgtcg tcgggatgaa caagctcgct catcttcaac
     2521 gactcatatt cctgctgggt atagccaata accgaataga aggcgggatt ggtaaattta
     2581 agcttccagt tcctgtcata aaatgaaatg ccgtcatcag ccgatctgat cagcatcctg
     2641 taaaacattt cagaattgct gtactcacgc tccaaggcat ctttctgcga tagcagttcg
     2701 ttatttgcct tctgaagctt ctggttgatg tcctctacct gtttatttcg tgcagtgagc
     2761 tccctgtttg tattccttag cctggttgaa aaaatgatta tcagtatgca tgctgctgcc
     2821 agcaatagta tcagggaaat gatataggcg ggcaacggaa caagggcaac ttcaagggca
     2881 cgtcccgaaa caatcggaac ctgatccgcc ggggcagcac tcagcctgta cgggaaaaga
     2941 aggctgaaaa ataaactggc tgaaaaaaaa ataaaccgga acgacgtgtt atttaattta
     3001 taccagttca tacatatcat ggatcaagga tggtgaatgt aaaaacaatt aactttcagc
     3061 attgaatatg ttgcaaatat aaaaaatagc ccgattactt gcctccggaa cgcaggcaga
     3121 atattacaca aaaattgtta aattgccaac aaaagaaagg gtaatggaac gaccacgaaa
     3181 tcattttacc aggggcagga gttataaaaa cgatgagata acggtttact ggaagcctga
     3241 tgcatgcatt catgcctcat actgctaccg tgagctgata gaggtatttg atcccggaag
     3301 aaggccatgg atagacttga gcggttctaa taccgaacgg ataattgaag ttgtgaacat
     3361 gtgcccgacc gaagccctct cctggaaatg gaacgaagat gcaagaaaca ttgatgtaga
     3421 aagagatcag accaatcata ttaaatttaa aaggcctgaa cttatgtcaa ccgaaaaagc
     3481 aaaggatgaa ataaggccgg tttcggttaa gatcatggtt gacggacctg tcgtcgtaaa
     3541 aggcaatttc accatcaggt ataacggaaa ggaaaaaaac ttcaccaacg gaatcatatc
     3601 actatgccgt tgcggaggtt cgaatcagat gcccttttgc gacggcacac acagaaaaat
     3661 cggatttgag gacaaataat gcctatggaa agaaatgaat tggatgaaaa caccggaacg
     3721 tcagctattg tagagataat tgacaacgga cccattaaaa tatcggggga aataatgttc
     3781 tgggatctta aaagggatat aacagatagt ccttcagaaa tttatctgtg cagatgcggg
     3841 aagtcatcca acaagcctta ttgtgatagt tcgcacaaga tgcgactgta atgttcctgc
     3901 cagattaaac accctgaact gatcttagta cctttccaac agcttctgca gcctccccga
     3961 aagaggtggc ggcaattacc gaaagcccgg aattgttaat tatctccctg gcttcctgtg
     4021 cattggttcc ctgaagtcgt accacaaccg gaacccttat ctttcccact gacttaaagg
     4081 cctctacaac accatttgca accctgtcgc atctgacaat accgccgaaa atatttatca
     4141 gcactgcctt cactgccgga tctttcagta ttatccggaa tcctgcttca acggtcgatg
     4201 cgcttgctcc gccgccaaca tccagaaaat ttgcaggagc tccccccgaa agcttaataa
     4261 tatccatggt tgccatcgca agcccggccc cgttcaccat gcatcccaca ttcccatcaa
     4321 gcttgacgta attcagatta tacctgccgg cttctacctc aatgggatcc tcttccctaa
     4381 gatccctcat tccgacaaga tcattatgcc ggaacaaggc attatcatcc aggataaact
     4441 tacagtcaac cgccagtatc tttccatcgc tcgtcttcaa aacaggattg atctccagca
     4501 actgggcatc acagcctgtg aatgtttcat aaataccttt caggaactta tgcatattcc
     4561 gtgaggcagt tccatcaagc cccagattaa atgaaatatt tcttgcctga aagtccagaa
     4621 gccccgttga aggggatatc tcctctgtaa atattttttc cggatgctgc tccgcaacct
     4681 gttcaatatc catgcctccc tcaggtgaat acataatgac ataccgtccc ttgttcctgt
     4741 taagcaaaat actgacataa aattcttcag gctcagattc tcccttataa taaacatcac
     4801 gggcaaccaa taccctattt accttcttcc cggccggacc agtctgcggg gtcaccaggg
     4861 tcatgccgat cattttttct gcaatctcac cggcttcctc cggagatctc gccagtttta
     4921 tccctccgcc tttgccccgt cccccggcat gtacctgggc ttttaccacg aataaccggg
     4981 atccgtactt ttcctgtagc tgttttgcaa ccaatacggc ctggctggct gtttctgcaa
     5041 caaaaccttc ctgaatttca acaccatatg cactcagaag cgatttcccc tggaactcat
     5101 gaatattcat cgtgtgttat ttacataaat taacttctta tcaatcactg atttcaatga
     5161 gagaaaatta aaaaaataaa tggatatttt gcctaattta gcatgaaaaa agtatgcgga
     5221 agcttcaaaa cagcgaactt ggacgaatca gtaccgaaca gttccgcaaa tcagaaaaat
     5281 caccgtttgt tatcgtactc gataatgtaa ggagccataa caatgtgggt tctgttttca
     5341 ggacagctga tgcattcagg gtagagaaga tctttctttg cgggatcacc gcaaaacctc
     5401 cccacaggga tatccggaaa acagccctgg gagcgactga atcagtggaa tggcactatc
     5461 atgaatcgac gattgatgcc atttccgaac tgaaggccgg gggctacact gttgtagccg
     5521 ttgaacaggc tgaaggatca gttgaacttc aggattttac aacaaaaagc aacaggaggt
     5581 atgctctgat cttcggtcac gaggtaaacg gagtggatca gaaagttctg gacctgtgcg
     5641 atgcatgcat tgaaattcct cagttcggaa caaaacattc attcaatata actgttagcg
     5701 ccggaattgt tctctgggag ctcaaccgta aaatatcaaa cctggtaaaa taaacagaac
     5761 cagcagaact gcagctgccc gtttgcaaaa aaaagaggtg ccccggaccg ggcacctcct
     5821 cctgtcattt ttttagtttc ctactgaacc aatcctttaa atgcatcatc agcaaagaat
     5881 tttgcaaatt cataatcggt tcttgcatag gctttcagat cagaattaag attaatagct
     5941 tccctgaggc cattaagcat atacgcttta tcatcaagac gggatccgat aattgcctta
     6001 agatacggga ctttccccat gcaggtctct gtcaggccgt caagcattgt ctttgccctg
     6061 ttaacatcgc ccttaaggta gagtgcaaga gcatgattat agcatgcctc atttccgaag
     6121 aaattaactg cctggtcgta ttcaccttta gtaattgcaa taactccaag gccccatctt
     6181 gattcaggtg ttgaggccgt cattgaatta aattcttcct cagcctttgc catatcaccc
     6241 tgaacaagat atacaaaacc tatgttgttc tttacaatat cattattctg gatagccctt
     6301 gctttctcaa atgcagcgag tgcttcatca gctttaccga ggcccagcaa tacatatccg
     6361 acattgttat gggcacgaat gcatttgggg aaattttctg cagtgatcct gtaaaactga
     6421 agtttttcat ttggatcatt agtcagggta gccgagtaaa gcatttcctc caggccaagg
     6481 gccttgggat ctgatttgat ctgagcaagg atctgctcat cactgcgacc gatcttatca
     6541 acgttggcag tcatttttga tcttctcagc tttggaagaa tgtcagtttt cagagcttca
     6601 taggctgcag acatgttttt tatctccctt tcacgtacaa ccggatctga gtacattgaa
     6661 agaaccctca gaatgagatc cttatctgcc agagatgatt tttcgacttc ggatttgaat
     6721 ccttcccagt cttcgggagt tgttaacgac tggaagaaat catcggcttt ggcggcttca
     6781 attttcctga attctctttt aacatacctg tcagctgaag ttccccgcct gaccgacaga
     6841 ttttcattaa ggtcaaaggg gccgtcagga gaagcatatg aacttacaac cagattcttt
     6901 aattggcggt tgggatcaac agtcacctga gtgatataat tctttaattc cttgatactt
     6961 tccttcctga gttctgaatt gcggatatca gcctgattga taacatataa tatgtcggct
     7021 gtctgtgatt caggcactat tctctgccag cgatcgggca taaggatcgg acgggcatgt
     7081 acatttacaa gcgttgaagt ggcaataaca ccatcagcaa gtttgaccgg atcgaaatca
     7141 aggcttttgt tgccctggct ggcctttgcc ctgagaataa gttccgacat tttcattgca
     7201 tccttataag gaattgaact tgtgtaagtg aagtctccgc cattgtaagt aataacctta
     7261 ttgttggcct gcactttttc accctgcaga acctgtactt tatcaaaagc cgtttctcct
     7321 cctgcatatg tcaggaccgg agcagcttcc agcaaagctt tcttgctgaa atacttttga
     7381 gggaatgtgc ctttaatggt aacatttacc attccgccat gtgcttcaag tactttgggt
     7441 gtaacttcat acttaatgtc accagcattc ttcttcatct tattcagacc gctgcagccc
     7501 gaaaatacaa cagctgcaag gataaggata aaataactct tgattttttt catagtttaa
     7561 tttgttattt gtgtttatgt tcccaggttg ttaaattaaa aattatcctt ctggattttt
     7621 gaatcaagtg caaatttata aaaaattgtt ttaatatcat caaaataatc aaattgttca
     7681 taggatgacc acattgagcc cggaagagaa ttctttgccg aatatcaaat cggttatcct
     7741 taaaaaatga tcctcccggt ttacaaaatc aagattgctg gcatcaatta ccagatatct
     7801 gttttcagga ttctgcctga aataggtgaa atagctgtcc tgaatcctct gaagatactc
     7861 cctggttata gacttttcgt atgaccttcc ccgcccggca atattggcca gcagtcgttc
     7921 gggcctgatg tgcaggtaaa cataaatgtc aggtttggga agtgatttgt agattatata
     7981 aaatatctgc ctgtagagat taaactcatc ccctttcagg gtgattgccg caaatacaag
     8041 ggatttcatg aaatagtagt cggcaaccgt aaatgattta aaaagatcag ctaatccaag
     8101 ttcctcattc agctgtttat atcggctggc cagaaaagag agctcaagcg gaaaggaata
     8161 tttatcggga tcagcataaa acctggggag gaaaggatta tccgcgaaag cttcaaggat
     8221 cagcctggca ttgtattttt tggcaatcat ttcagcaagc gatgtcttac ctgctcctat
     8281 attaccctca atcacaagat aattgtactt aaccttcata atggtctgat aacaatccaa
     8341 tttaaataat tgaaacggga attgcaacaa aacccggccg gatcagcagg gtttcaatct
     8401 aaagcaatat gtacggtatt gagttactgg aaaactcccg gcaaaaccgg gggctgcagg
     8461 gagaaaaacg atctggtgcc gggattaatg cctcttatct tccggccttt tatgcccgcc
     8521 ttcctctttt ggtggccggg gaagaagtac tttccttgaa agcttcagtt tccctgtttt
     8581 aggatctata tcgatcagtt tcacttctac ctcatctcct tcctttaaaa cttcatcaac
     8641 tgttttgatc tttttccagt caatttcgga aatatgcaga agtccgtcct tgttgggaag
     8701 gatctcgaca aatgcaccga actgcacaat tgttttcacc tttcccctgt acacctgccc
     8761 cacctccggt acagcaacaa tctttctcac ccattcaaga gcggcaacaa tagcatcctt
     8821 gttttcacca aagatgttta catcaccatg accgttgatt tcttcaacta ttattgttgt
     8881 tccggtttca cgctgaatct cctgtatcac cttgcctccg ggtccgatga gtgcaccgat
     8941 catctctttg ggtattgtca gcatttctat cctgggagca tgaggtttaa gttcggggcg
     9001 aggctcggag atggtacgat tcatgatgtt gagtatgtga aggcggcctt tttttgcctg
     9061 atcaagggct tttgcaagaa tctcgaaaga gaggccttcc acctttatgt ccatctgtgt
     9121 agcagtaatc ccgtcacgcg tacctgcaac cttgaaatcc atgtcgccca gatgatcttc
     9181 atcgccgagt atgtctgaaa gaattgcgat cttatccgaa tccttgtcgg ttatcagtcc
     9241 cattgcaata cctgatacag gcttcctcag cctgatcccc gaatccatga gtgccagtgt
     9301 cccggcacaa actgttgcca ttgaagatga tccgtttgat tcaagaatgt ctgaaacgat
     9361 cctgactgca tatggattct cctcctcggg aggcatcatg tttttcagag ccctgtgtgc
     9421 aagattgcca tgtccaattt ccctgcggct tattccgcgt attggtcttg cctcaccggt
     9481 agagaatgga gggaaattat aatgaagcgt gaatttttca gtaccctgat taagtacatc
     9541 atctatgatt ttttcatcga gcttcgtacc cagcgtaact gttgtaagtg attgtgtttc
     9601 accgcgcgta aacagtgctg aaccatgagt ggccggcagg ggatcaattt cacattcaat
     9661 tggcctgatt tcgtcgggtt tcctgccatc gagccttaaa ttttcatcaa ggacaagcct
     9721 gcgggctgcc tccttgtgta cttcatgata atattttctg acaagagttt cattgatctc
     9781 ttcctcttcc gggaactgtg caagaaattc agctatcacc gattcaaatg cctcttcgcg
     9841 cctgtgcttg tcagcagtaa gcgatctggc cacctggtag cacttgtcat aggtttcgtc
     9901 ccatacacgt tttctgagtt ccgggtcatt tacttcatga caatactccc gtttggtggt
     9961 tgcacccagc tcttcagcaa actcaacctg ggccctgcag aggattttaa tcgctgaatg
    10021 agccactctc agggcctcca gcatatctga ttccgatact tccctcattt caccctcaac
    10081 catgagtatg ttctcatatg tagcccctac cataaggtct atatctgagt ctttaagttg
    10141 cgaagcagta ggattcacca cgaactgacc gttgatcctt gccacacgca cttctgccat
    10201 gggcccgttg aaaggaatat ccgaaacggt aagtgcggcc gaggctgcaa gcccggcaag
    10261 tgcatcagga ataatgtcta aatcagctga aacaagattt accgtaacaa aggtttccgc
    10321 atggaaatca tcgggaaaaa gaggccttaa agcacgatct acaagacgtg aaataaggat
    10381 ctcgtaatcc gaaggccttg cttcgcgttt cagaaatcct cccgggatgc ggccggatga
    10441 tgcgtatttt tccctgtact cgactgaaag aggcatgaaa tcagtatctt cctttgcttc
    10501 ctttgccgca acaacagtag ccagcaacat tgttttcccg aatttcagaa ggacagatcc
    10561 gtctgcctgt cttgcaagcc ttcctgtttc cagcgtaatg atcctgccat caccaagatc
    10621 aatattcttt tcttttatat taaacatgtg taataagatt ggtaaatgaa catatcgcat
    10681 ctgacaacag agaatacaga aaaaggcaac ctgaaattgc ctttttccct attttcttaa
    10741 tttcaatgct tttacgattt cgcgataccg ttcgatatct ttaaccttaa ggtaatccaa
    10801 aagccttcgc cttctcccga caagcttaat cagagctctc tgggtgctga agtccttttt
    10861 atttttcttg agatgttcag tgagatggga tattctgtat gaaaataatg caatctgccc
    10921 ttccgctgtt ccagtatcaa tctccgatga tccaaacgtt ttgaaaaact cctgcttctt
    10981 gtcagtagtc aaatacatgt ccttaattaa tattgtgttt atccataaat aaattgagtc
    11041 accaattttg gaacgcaaaa ataaggcttt tttatgtaaa taatcaaaca attgaataat
    11101 ttttttggtg ccggataaaa atacctgaat taaaaacgtt cgggaaattc cgattattgt
    11161 tttatcataa tgacgtaaaa ttagattaat acgctgcgtc ggcaaattat aaattcatgg
    11221 aatttattaa cagttatcaa cagtttcgaa ccagtaatcc ttccccgtaa aacacttctg
    11281 actgtcagaa gtttaattgt catggctcaa ggagtaattc ctcctgtttt ttcagatctt
    11341 ttcccgcact gattattact gcccctatcc tgcattcaag gcatcttctt tttttgcagt
    11401 attcatccct taaatggata agagcctgtg tatcaaaagc tgatcctgct tcaatccccg
    11461 atttcctcca gtctgcaata acagaattgt cttcggcatg caattcgtcg agaaaatcaa
    11521 gtgccttccc ccatattcct tcttttcctg aatactgacc atatacaaaa atcaggggga
    11581 tcacggcatt gacaagtaat aattctgctg cctgaaggcc gacagatttg ggaactgatt
    11641 tgcttttgcg gccaaagatg taatgatcat cccagtatgg tgaagaggta acctcaaaca
    11701 attccctcag ccggcagatt tcctcagctt caagcaccct tgaaaacaat ccgcctgcaa
    11761 ctgaaagcat cgcggcgagc tgggaaatcc tcaccgtagg aaagtttacg ggccgcaacc
    11821 tgctgaattt ccaaagccag ccgtgaatgg gaacaatgga atacttggca gacagaatcc
    11881 tgaattccct cacaagctgg ctgtaataat catctgcaat agcattttta aatagtcctt
    11941 cctccagcat tccggctgtt ccaaaaagca gggcctcaac ctgcaatctg ttgtcggcat
    12001 gttttcgtat tattttgaag ggcaatgcgg ttgcaagcat ctcaaaggga tccgtattta
    12061 ccctgaaccc gaaatagcgt gtcagtatcc tgtaaaatgt ttcatcccag tcattaccgg
    12121 tatccctgaa agttctgaag atagcggctg atttttgctg caatctttct gccagcaggt
    12181 aatggagcca gtgccggatg atgaacctgt ccatcctgtt aacatagccc tggcaggcca
    12241 ctacggccgg attgtttacc agatcaatat acctttgata caatgcagca tcatacttga
    12301 tttcaactgt cagcaattca tttcccctcg cattgaaaac ctttttatca ttctcaccga
    12361 caacatggag aatgacattg tcaaatgcat gatcaaggtt atggccatgt gcattgaagt
    12421 gactcgaccg ggtatgtatc tccacattcc cggcccattc tgttccccct atgttgatcc
    12481 tggcgttgaa aaaatcagga cctgaatcac ggttataatc gccgggataa ataacaataa
    12541 ttctgttttt ttcatgatca acaagtgcat ccttgtcata aagtgcatac ttccacaggt
    12601 aatggaggaa ttcttctttc attgcagcac gatttaaatt tgacagcgag atcataaata
    12661 taatgaaaaa ggcctgaaaa aagaaacttc aagccttttt tctatccatc acgcgggaca
    12721 ttctgctaaa cagcacgatt acaggccatg ggcctgctga ccaggtccac gggaatccgg
    12781 cagagacagg ggcagtctga tgacctaaat caggcctgct tcggcaaggt attccgccat
    12841 ttcctgctgc agttccatag ccttcctccc tgctacgtta tcaaacttat ctgagttgtc
    12901 ggcaaaaatt atacctctcg aggagttcac caaaaggccg cagcggtcgt tcataccgtg
    12961 cctggcaacc tccctcaggc ttcctccctg ggcccccacg ccgggcacca gcaggaaatg
    13021 atcgggcaca atttccctta tccctgcaag catttcagcc ctggtggctc cggccacata
    13081 cattaaatta tcaattgttc cccaattccg tgaggtccgg attactttct caaaaagcct
    13141 catcccgtca ccgtaatgat attgaaaatc atcagcgccc ttatttgatg tcagtgcaag
    13201 gagcacaacc catttgccct gatatgaaag gaaaggtaca acagaatcct ctcccatata
    13261 tggtgcaact gtgactgcat cacattgcat cttttcaaaa aaggcctttg catacatcct
    13321 ggatgtgttc ccgatgtcgc cccgttttgc atcggcgata ataaaaatgc cgggatagtt
    13381 ttccctgata taggatattg ttgattcaag cgaaatcaga cccctgtatc cgagggtttc
    13441 ataaaatgcc aggttgggtt tgaatgccac tgcatagccg gcagttgcat caattattct
    13501 cctgttgaac tcaaaaaccg gatcgtcata cctgtggagg aattcaggga ttttttcaat
    13561 ttccggatca agtccgacgc acagaaatga ctttttggcg acgatattcc tgaacaatcc
    13621 gttatgatcc atgtctcctt aaatattgtt ttcctttaat ctttctgcat tttctgccat
    13681 ctgcagcttg tcaatgatct cacctatatt gccgtcgagt atcgaaggaa ggttataaag
    13741 tgtcagattg atcctgtggt cggtcattct tccctgggga taattgtatg tccggatttt
    13801 tgcggaacgg tcacctgttg acaccattgt ttttcttttc cgggaaacgt catcaaggta
    13861 tttctgatac tcgaggttat aaagcctggt cctcagttcc ttgagagctt tgtcataatt
    13921 cttgatctgt gatttttcat cctggcaggt gaccacgata ccggttggga tatgagtgag
    13981 tcgtattgcc gaataggttg tattgacaga ctgtccgcca gggcccgatg agcagtaagt
    14041 gtctttccgt atatcctcat tttttatctc gatatcgaat tcgccggcct ccggcagaac
    14101 aacaacagaa gctgccgaag tatgtacccg cccctgggtt tcggtctgag gcactctctg
    14161 gaccctatga acgcccgatt catatttcag tgtcccgtaa acaccttctc ccgagacact
    14221 tatcacaatc tccttataac ctccggcggt tccaagcgaa tagctgttta tatcagcacg
    14281 ccagcccttt tgctcaaaga attttgaata catcctgaaa agatcgccgg caaaaatact
    14341 tgcttcatct ccgcctgttc cggccctgat ctccattacg gcgttctttt catcttccgg
    14401 atcggcagga atcaacagga gttttatttc ttcctccata ccgggtaccc tgtcattaag
    14461 ctcttcaagc tcagcttttg ccatttccct catctcttca tccttttccg atgccagaag
    14521 ttcctttgta ctggcaatgt tcgcaagggt taacctgtaa ttttcatatg cagctattat
    14581 tggctgaaga tccttgtact ccctgttcag cctgatatac ctttccatat ctgaaagcac
    14641 atcctgatgt gtcagcagct ctccaacctc caggaatctt gcttttacac cttcaagctt
    14701 ttcaagaatt aaattgtttg ccatttcctt caaatttcag ggtgcaaatt tagaaaattg
    14761 tatgcatatc tgatgaggca ggaagtttgt atcagccgga actactactt aaatttcaat
    14821 cttcctgatc tgatttaagc ttgaacgggc atttgcctgt ttgtccgaac cgccttaaag
    14881 gaaaggaatg gttcatattt tgcaaatcgg attaacatcg gccctgtaat tttctagtat
    14941 tcaaatttcc ccggctcaga aacaattgta agccttttct ggtccgattt ctcgcaatgt
    15001 ccgatgaccc tggcatccag gtcaaaccct ttagcaatat caatcacctg tgaagcatct
    15061 tcctcacaaa gataaatttc aaatctgtgc cccatgttaa atacccggta catttcgctc
    15121 cagggagtac ctgactgttc ctgtatgatc ctgaaaagag ggggtatact gaacatagaa
    15181 tccttgacaa tatgcaaatt atcgataaag ttgaggatct tggtctgtcc tcccccggaa
    15241 cagtggatca ttccgtggat cctttccctg aatgcttcaa gtattgcttt gattaccggg
    15301 gcatatgtac gggtgggaga aagtaccagc ttgccgtaat caaggctgaa accttctggt
    15361 atgtcagtca atgccagtga acctgaataa acaatatctg aaggcaggtc agtatcataa
    15421 ctttcggggt acttttcagc caggtaatga gcaaaaacat catgccgtgc cgaggtcaga
    15481 ccgttactgc cgattccgct gttatattct ttctcatagg ttgtctgtcc ggacgaagca
    15541 aggccgacaa tcaggtcacc gggcctgata ttgtgattgg aaataacctc cgaccttctg
    15601 accctggcca ctaccgttga atctacaaca atggtgcgga caatgtcgcc cagatcagca
    15661 gtttctccac cggtagacct tatatctatt ccgaggctgc gaagatcttc cagcacctct
    15721 tcagttccat tgatcagggc tgaaattatt tctccgggaa tcagtctttt gttccttcct
    15781 atcgtggtgg acaccagtat attgttgacg gcgccaacgc agagcagatc atcggtattc
    15841 attacaacag catcctgtgc aatacctttc cagaccgaca tatccccggt ttccctccag
    15901 taaacatatg cgagggaaga ttttgtgccg gcaccgtcag catgcatgat attgcagaaa
    15961 tcaggttcgc ctcccagaag atcgggcact atcttacaga aagccccggg gtacaatccc
    16021 ttatcaattc cattaatcgc tttgtggaca tcttccttcg aggacgagac tcctcttttg
    16081 ctgtaccttg actcttctga cattccctgg gttattgtta ggcaaagata gaaaaaagtt
    16141 tattcatgtt ttcgcccggc ccttccggca gataatccgc gatctgtctt cttttaaaca
    16201 ccatctgtta aataaataac agcagctgtc aaacctgcag gaataatttc ggccggattc
    16261 aatgacaggg aatcagggtt tacagacagg atacagtgga aaagagaact attatgagtt
    16321 ccggttattt gcccgaaggt tcatagtcgg tcctgagtac cctgaattca gttcttctgt
    16381 ttatctgatg tgcaatttcc ttctgttcct cacttcccag gctgttgatc agttgttctg
    16441 tcagaactgt tccaggcttc agaaagctgt tctgggcagc tatggcatca tcaaccacct
    16501 tgggggtgga ttcaccgtaa ccttttggaa acagtctttc cggagcaatg cccttttcaa
    16561 taagatactg aactactgac tgggctcttt tctgcgatag ctcgagatta tattcttccg
    16621 tatcccggga gtcggtatgg gacatcagct ctattgtaac gttgggatta tcgttaagcg
    16681 tttcaaccag tttgtcgagc gacaccatcg attcaggccg caaatcccat tttccgaaat
    16741 catagaatat attgggcagt tcaattggcc tgtcaatggc tgtaagcaat attgtaacca
    16801 tgaagtcgcg gcttttttcc tgacccttgg tggtttcttt ttccttcccg ttcagatagc
    16861 ctttctttga tgcaaggaat atataatcaa catcctgttt gagactgaat ttaaactcac
    16921 ctccggttcc ggtttcagcc tgaaggtttg atccgtcact tgcaatgagc tgaataaccg
    16981 atccggcaat agggctcccg gtcttttcat cctttacgag ccccgtaacg gagaactgca
    17041 gcggcggaag ttcaaaggaa aagagatcat cattacccct tcccttcctg gttgagctga
    17101 atattcccct ttcgcttccc ttctcaaaac agatcccgaa atcatctgca ggactgttta
    17161 ttggtgattt catattggtt accacccatg aaccatcggg ctggggtacg gctttgaaaa
    17221 tatcgaggcc tcccatgcca atcctcccgt cggatgcaaa ataaagtatt ccgtcttccc
    17281 tgacataggg aaaaagctca tttcctgaag tattgatgtc ggggcccaga ttgcggggag
    17341 gggcccatga gttatctccg ctttttgttg tgtaatatat gtctttctgc ccaaagccgc
    17401 cttccatttc tgaaacaaaa tacaggatat ggccgtcagc tgacaaggca gggtgggcag
    17461 caaccagcga atcaggcaga atcttaatat cctgcggctt gctccatctg tccccgtttc
    17521 ttttggaata catgattatg caacccttct tttcacgctt acctgcttca caccttgtaa
    17581 agtaaacctc agagaaatcg gatgaaaagc agggcgtccc atcttcaaac tcgctgttaa
    17641 gatcggttac aggcacagga gtgctccatt tggatttttt gtcgagccgg ctttcaaaaa
    17701 tatcggtaaa attctggcca gttgcaccgt gggttttatt tccggcagca tcatcacgcg
    17761 atgaagtgaa gtaaacgagg ctgtaatcat cacggccata ggcagggctg aaatcagatt
    17821 tcctcgaatt aagatcctta agctcctcaa ccttataagg ttcgggattc ctttgccact
    17881 ccagggcaag ctcgcaggcc cgtatttcct ggtcagcctt tatatcggtc ggggcaattt
    17941 gcttgtactt gcggaactct tcaattgcct gctgatattt cccgtttttt ttcatggttg
    18001 aagccagcca gaatgatgcc tcaggctttg agaacgagga ttttgctgca agccggtacc
    18061 aggtttccgc atttttcgga tcattggtga gcctgtagca ctcggcaatc atgtaaccaa
    18121 gttcggtcct agcggccctg tctgttttac tcgatttgga atatgcatcc ttgaaatgat
    18181 caattgcttc ataatattca cccgcgttat acgatgcata ggctctctcc gttttccgct
    18241 tctgcgccga accgtcgggt gatgccatga aagtcagtaa gataataaac aacagcactc
    18301 ttctcataac ctgattatcc atttggcaat aaaagtaatc attttaagca attatggtgc
    18361 cagaatttaa cgacgggcga aatgaaatat tttggctgaa aaggaactgc gcctgatatc
    18421 cccgtaatac atattttata tgtcgccccg gtcagcattt cagtccttgc cacaccatgc
    18481 cggctgccgg gaaacaacgg aatggccccg ggaaagtgat tcctgaaaaa tccggagatt
    18541 gcaaattata ccggttatct gctaccttgt aaaaagcagc tcccggtatt tcggcaaggg
    18601 ccagatctca ttgtcaacaa tcagctccag cttgtcgata tgatatctta tcttgtcaag
    18661 aaatggtttc acattatttt catactcaat ggcctttata taagaatccg ttattatgtt
    18721 ggctttcttt cgagcttcaa ccatttcatt ttcaagtttc tttatggcag aaatatgatc
    18781 tgaaatcctt attatcaaat ccttgctttc ggctgccagt tttgaaaatt cagcttcatc
    18841 aaaaatctcc ttcagtcccc tgacattttc aatcaggctc gtcatgtatc tgatacctgt
    18901 aggcacaata tgatttatag ccagatcacc cagaaccctt gcctcgatct ggactttttt
    18961 tgtgaatttt tcgaattcca cttcaaccct gccttcaagt tccttttccg aaaaaattcc
    19021 tgtgctgatc agaacctccc gggaattacc ggcaagatat gccttcaacg aatcaggaac
    19081 gctggcaatg cctgaaaggc cgcgccgggc agcttcatca tgccagtccc tgctgtagtt
    19141 atcaccttca aacaataccc tctgcgattc ctggatatac ttcttaagta tctgaaaaat
    19201 ggcttcatcc ttgtttctgc cttttctgat aagaagatcc acctcctttt tgaatcttac
    19261 aagctggtat gcaacagcag cattaagtgc aatcatggct gcactgcagt ttgcagaaga
    19321 gccgaccgtt ctgaattcaa acctgttccc tgaaaaagca aatggcgaag tgcggttgcg
    19381 atccgtatta tcgagcaaaa tttcgggaat ctttccgatc ccgagcttga gttcagtttt
    19441 ctgcgcaggg gtcattttcc tgtctgtaac cttatttact atatcatcaa gcataccggt
    19501 aagataactg ccaagaaaaa ctgaaataat cgtcggcgga gcttcgtgac ctccaagcct
    19561 gtatgaatta ttctcactca ttaccgaggc cctgatcaga tcattactgt cgttaaccga
    19621 ctttataaca ttgataagaa aggtgagaaa ttgcaggttt gacttgggat ttttccccgg
    19681 cgataaaaga ttctttcccg tattggtctg catcgaccag ttattgtgct tgcctgaacc
    19741 atttattccg gcaaagggct tttcatgaaa caggaccctg aatttatgtt ttcgtgctat
    19801 ccttttcatc acatccatta aaagctggtt gtggtcgacg gcaagattaa cttcctcata
    19861 aatgggggca cattcaaact gattgggagc cacttcgttg tggcgtgtct ttatagggat
    19921 tccaagttta tatgcctcgc attcgaaatc ctcaatgaag ctcattatcc tttccggaat
    19981 ggatccaaaa taatggtcgg acagctgctg atcctttgca gactggtgtc ccatcagggt
    20041 tcggtcggcg aggaccagat ccggtcgggc ataatacaat gcctcgtcaa caagaaaata
    20101 ttcctgctcg caccccagcg ttgcaataac ctttgtaaca tccctgtcaa agtaccggca
    20161 gacggcagtt gcagccttat caagtgcaga aagtgcctta agaagcggcg ccttgtaatc
    20221 gagagcctcg cccgtgtagg atacaaaaat cgtagggatg cacagagttg cacctacaat
    20281 aaatacaggc gatgaaacat cccaggcggt atatccacgg gcttcaaaag tattccttat
    20341 cccgccgctt gggaaactcg aagcgtcagg ttcagactgg actagcacct caccggaaaa
    20401 attctcaact atatgtcccc tttcacccat ttcaatgaat ccatcatgct tttctgccgt
    20461 cccctcagta agtggatgga accagtgagt ataatgggta gcaccccgtt ccaatgccca
    20521 tgccttgagc cctatcgcta cctggttagc ttcctccctg gttagtgatt ttccaagctc
    20581 aatagcctgt tttaccaccc tgaatgcttc tgcagaaagg tagtgctcca tcttatgaag
    20641 atcaaaaaca ttaattccaa aatatttgga aacattcttt tcaggagtcc ttacggtaac
    20701 aggttccctg ttaaatacct ccctcagtgc actgaatctt aattcggcca ttttattttt
    20761 tttgcgatta tataaatatt ttggatacac cccccatatt tcttcaactt ttttaataaa
    20821 aatgatcatt tcaccggaca acccctcttt ttttagtacc agaacttata tttttgcata
    20881 ttttttcatg cctgcccccc tgtccggaag ggatacattc atatcccgtg caagcgtgga
    20941 acttgcgggg aaatcacaat caccgcatct gatttattta tggaaatttg ttatatttgt
    21001 cgcctgaatt tttacaggca attatcaatc aataaaaaca gcttattgaa tgtcagcatt
    21061 acaattttta agggaaaaag ccggggttct tgttgcagta gtaattggaa tttcattgtt
    21121 cattttcgtg gtaagtgact ttttcggggg tggaagagga cagcgcctaa gggcaaaaaa
    21181 atattatgag attggcaaga ttggcaatga aacggtctct tatcaggatt atgagcagaa
    21241 agttcagaat ctgttggaca tttataagct gtcaggaaat acaagtcccg atgcaaatac
    21301 tgttgaatca atcagggagc aggtatggca acagatgata agggaagaac ttctcgacaa
    21361 acaatatgtg aaactcggaa tcggtgtaag cagtgaagag gttgatgaac ttgtactggg
    21421 caacaatccg catccaattg tcacacagct ttttactgat caggagacag gtttgttcaa
    21481 taaatcgttc ctcgtaaatt tcctgaagca gaccgagctg gatgaaactg caaaaagata
    21541 ctggctgtat tttgaagatg aaattgtaaa tgacaggatg agttcaaaat acaatacgct
    21601 tgtatccaag ggactgtatg tatcatccag acaggctgaa tttgataaaa atctgaccgc
    21661 ggcaacggtt gatttcagtt atataatgaa aaattattcc tctgtacccg attctcttgt
    21721 aactgtttcc aggggcgaaa ttgaatccta ctattcaagg cacaaggaaa attacaaaag
    21781 aacggctttg agggatattg aatatgttac gttcgatatt gttccctcag aagaagatat
    21841 aaaacaggct gaagagtgga tcaataaaac caaagttgaa tttgcagagg caaccgatcc
    21901 ggagcagttc ctgaatctta cggcagatac caggcatgtt ggtttttatg aacccattag
    21961 ccagattcct gaaaaccttg tggagtttgt aaaaaaggaa aacaccagtg aggttttcgg
    22021 tccctatgaa gaaaacggaa cattaaagct ggcaaggatc ctgaatattg ccaacaggcc
    22081 cgattcggtc catgtccggc atatcctcat ttcaccgaat tcatcaggat cgctggatca
    22141 ggccaggtca aaggctgaca gtcttgttaa actcatcagg tcaggtactt ccttttcctt
    22201 ccttgcaatg tcaaattcag atgacagagg ttcgtcacag ctgggtggtg atctcggctg
    22261 gttccccgaa ggaaggatgg ttgtcccatt caacaatgca tgtttctcag gaaaaaaagg
    22321 tgatatactt actgtagaaa cttcatttgg tatccatata attgaaatcc tggaacaatc
    22381 gaaaaacatc agaaaataca atattggaat aatcgacagg aagataattc ccggtcctgt
    22441 cactaaccag cgtatataca gtgaagccag ccagtttgcc ggtacgaaca atacctatga
    22501 aaagtttaac caggcgatcg ccactgaagg cctgacaaag agagtggcaa atgatgtaac
    22561 accaatgcaa aaaactcttc cggatcttga aaaccccagg agcctcatta tttccctctt
    22621 ccagactgaa aggggtcata ttgtgctcga caacaaccag caggcagtat ttgagatagg
    22681 tgaaaaatat gttgtggctt tctgtacaaa ggtgcaggag gaaggcattg cacctgtgaa
    22741 ggatgtggaa aacgaaatca ggtttgttct catcaaggat aaaaaggcag aaatactttc
    22801 agccgaatat aaaaagaaca tgcaggaagg caaatcactt gatgaactgg cttcggacat
    22861 gaaacttaca gtcagggaag caacccaggt gaatttcaga tcatattcca ttcctgacgc
    22921 aggcagtgaa ccggcactca ttgctgctgc ttcagccgca aaacaagggg tactcagcgg
    22981 tccggtaaag ggagagaacg gaatctacat gcttaccgta aacgaaattt ccaattccga
    23041 cagtgatgat atcagatcgc ttaaggacag gctttcaata acataccaga tgagaggcag
    23101 ctacgaggct tatgaagctc tccggaaagc tacaaacatc gttgataaaa gatataagtt
    23161 ctactaggag cagcaaaggt gattccggtt cagagacgaa acctttcaat aatctgatcg
    23221 tcgtccattt ccttggccag tcccttgata taatcgggtg gaactttata cagatccagt
    23281 atcaataaac cgtcaaggca gtcattgaat ttcggatcaa tattaaaacc aattatgcgg
    23341 ccattgatct ccaggtattt tcttaaaaga acggggatcc tgtaaccagg ttcgatattc
    23401 attattatcc tctcaatctt gtttatgtca tgatcggcaa catctatgaa gatcttctga
    23461 tcaacaatcc tgtcatatct cacaacatat ttttttttcg gaataatggc cctgcccatc
    23521 ttctcatcat aaaaataagc cttcacaaat ccgactatca gattttttga aagtttcgta
    23581 aaatcattgc ttatacttac aggaccgata aggtaacggt attcctggtt tctcaggaga
    23641 aataccataa tgcccttcca taaaaggaac aggggtatgg ctttcttctg gtaatccttt
    23701 acaatgaagg atcgtcccag ctcaagggat tctttcaaga cgggaagaaa gctgtttctt
    23761 attttgaaaa gtgaattgat ataaaaaccc cttatcccat aaatgtttgc aatctccctg
    23821 cctttcccta tcctgtacga tccgactatt ctgtccgcat cattatccca tatgaaaaga
    23881 tgattataat agagatcata ttcatcgata tcagtacttt tatttgtccc ctcgcccact
    23941 tccctgaaag tcagctccct gagccttcca atttcattta tcaggtgggg aatgacacgg
    24001 gttggggcac aaaccacaga ataattcttt gttgaaaaaa gttcatactc gctttttatc
    24061 ctttcatatt cttccctgag cagctggcct gacaacgggg ctgcgaccgg ctcgggattg
    24121 accttcctga cagccggctt aaggttgaaa aatcttttaa cttccagttt tgagcccagt
    24181 aggtaggtcc ttgccctcag gtaccggcca tattgggaaa tatctttaaa ttcttcctgt
    24241 tctctgactg ttattggtgc acctatcctg atccttacaa tcctgttttt cttgttcagg
    24301 atctctgaag gcagatcggt aagtttcatc aacggattta tcttcctgaa aatatattgt
    24361 aatcttatct gtgttccgtg aaaataaacc gggattaccg gaacattggc atttttgatg
    24421 aattttatgg cggattgctg ccattctctg tcaagtatga catgtgaatc ctcaaactgg
    24481 gcagcacttg cacctgcggg aaatattccc aggcagtgcc tgctttcaat atgtattgga
    24541 gcttcattga aatcagtgaa aagcgagttt attcctgccg gattgaaaga gtcagtcata
    24601 gataagttgg agctgtcaaa tgattcaata cccggcaaca aataattcct tactattttg
    24661 aaatcgttcc gcttagggta tatcagtttg taaagaagca gggtgtcaat tcccctgtaa
    24721 ggatgatttg agatggtaat aaagcttcct tcaggctgaa tgttttccag gtcttcgtca
    24781 ggaatatcaa atttgagttc cagttcttca agaagcgaat tgataaggaa tatattatct
    24841 tttccgtaga gtttccggtc aagtttactg agttgcttgt ggctcaggat ccgcagaagt
    24901 gtttcttcaa ttacaggccc ttccggtata tatatcctgt caggattttt aatttcgtcg
    24961 tccgaaatca gtttcatagc tgggaagtat tatatcaaat atagtcatta taatccacaa
    25021 ctgatccggc tgatacgcat caaggaggaa aaaccttcgg aatgagtttg ccaggaccat
    25081 ccctgttttt acaggtgtca gttctctttt tatggccgtg tccggggaaa gcgggataaa
    25141 acaaagagcc cctcagtttc ctgaagggct cccatgataa ggccggcaac taccggattt
    25201 taccccgaga actcggggct cccgcattta gcagcacctg tcgttctgta tttcacgtcc
    25261 cgtctccggg gaaggggtat aaaacaaaga gcccctcagt ttcctgaagg gctcccatga
    25321 taaggccggc gactacctac tctcccacaa ttagcagtac catcggcgct gacgggctta
    25381 acttctctgt tcggaatggg aagaggtgga tccccgccgc tattgtcacc ttagaacctc
    25441 tgagactttt gacgtcaatg aatcgaaaaa cacaatactc tatttattgt tatatcaaac
    25501 gtcaatctca aatatcttga catactggcc ggaaaatcag taagatccta cacggaaaat
    25561 ctttcgggta attagtactg ctcagctttg acattactgc ctttacacct gcagcctatc
    25621 aacgtcgtag tcttcaacga ccctcaatgg agatctcatc ttgagacgag cttcgcactt
    25681 agatgctttc agtgcttatc tcttccagac atagctaccc tgcgatgcag ctggcgccac
    25741 aacaggtaca ctagtggtct gtccaacacg gtcctctcgt actagtgtca ggttctcgca
    25801 aatctcctgc gcccacaaca gatagggacc gaactgtctc acgacgttc
//
LOCUS       DAOOYG010000026        25605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1717_length_25605_cov_7.332250, whole genome shotgun sequence.
ACCESSION   DAOOYG010000026 DAOOYG010000000
VERSION     DAOOYG010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25605
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1717_length_25605_cov_7.332250"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..1513
                     /locus_tag="PK288_04025"
     CDS             383..1513
                     /locus_tag="PK288_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18119.1"
                     /translation="MNKLIAIVLLVFLASFTRGNEPKIPCYAWLDGPGKATDTRLKAQ
                     FTDLRSKGIDGLMYTAGRDPEVYRRVGKIAKEAGLEFHAWIPTLIQSNNPDMKPEWYA
                     INGLGESAYDKPAYADHYKFVCPNRKEVVQYLENLYGTIAGIPEVDGIHLDFIRFPDV
                     ILARGLWKKYNLVMDREYPQFDYCYCDKCKNDFLINTGVDLNDVEDPSQVEKWKQFRY
                     DLITDLVNRLADAVHSKGKKITAAVFPGPNSIAKKIVRQEWDKWKIDAIYPMNYNDFY
                     LEGTKWIGDMCREGVTSLNNSKPLYSGLFICPDPAGKANDPDPENHGLVPGELEAAIR
                     ESMENGAAGICLFTPDRMTSEDWVIFKKVIHNKYQNKVLPAK"
     gene            1930..2802
                     /locus_tag="PK288_04030"
     CDS             1930..2802
                     /locus_tag="PK288_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18120.1"
                     /translation="MGILKRHAIFILLLLIAFILLANKLFIWGFVALGIGLIIFGIQQ
                     LIGLNVKLSKFQDRVGGLEEKNKSLLNLNRKLEEEVSFLKERQFQITKIRNILELNLF
                     EVETTFNRPVSRKVTSEDDREIKYFGSLNISLGAKFGIDCKELRFKYLPENDELIVAN
                     INPRFLSFGKRKLEWDFFEIFEFRSQNALAEKRWMTTDNLYKYASKVKEEYRLNTEKS
                     LEKGPEELEWIHKPIMQTVINTIRAVFGSLCKNITIAETADETFVPIEKIRFDPLQDA
                     KEIQPAEKKALKES"
     gene            2960..3757
                     /locus_tag="PK288_04035"
     CDS             2960..3757
                     /locus_tag="PK288_04035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM18121.1"
                     /translation="MPETIISNQGAGSADEALYPDYFARFYDLIYHQVRDDVDNEYYL
                     KKAVGTKGRILEAGVGTGRLFTATLDNGADIYGIDISPAMTDILKSRLNDSQGSRITC
                     QNIIDFKFDFSFDLIMAPFRVFMHLTEKSDQIRALNNVYEHLNPGGRFIFDVFVPDLQ
                     ILISGLNNVIDFDGEFEPGNRLKRTVTTRPDLINQLINVTFRFDWNEGRSIFTKEWNT
                     SLRYFFRFELEHLIERSEFDSYTICGDFEESELSPESKEFLISCMKC"
     gene            3925..5010
                     /locus_tag="PK288_04040"
     CDS             3925..5010
                     /locus_tag="PK288_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPM18122.1"
                     /translation="MQAQNKRAADYGIRFGVLNPGRNNAITDVPGVKVGHVTLKEGNA
                     VNTGVTAILPHSGNIFQNKVPAGIFAGNGFGKLTGVSQVEELGNIETPVILTNTLSVP
                     VAAEALIDYTLGLPGNITVMSVNPVVGETNDAGLNDIRRRVITREHVLNAIAAADTGP
                     VAEGNVGAGTGTVCFGFKGGIGTSSRILPESKGGYTVGVLVQTNFGGILRINGIPVGI
                     ELGRHKFMKITEKYKEDGSCMVVIMTDAPLDSRNLKRLAARAMGGIAMTGGMTSNSSG
                     DFAIAVSTAENLRIPWSAKSMYQEMTVLRNEEMDLLFEAVIESTEEAIINSLFAAETV
                     TGRYNFRIEALPVEKTLEILRKHNIIK"
     gene            5027..7240
                     /locus_tag="PK288_04045"
     CDS             5027..7240
                     /locus_tag="PK288_04045"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPM18123.1"
                     /translation="MNRIPGLCTVIVTAILISSSISCSQKTPDPVDYVNPYMGNISHL
                     LVPTYPTVHLPNSMLRFYPERDNYTTNKMAGFPLNVVSHRSGTVFMIIPFSGSNAGEQ
                     ALTPFTTDNEKISPYRYSVTLDERNIGLEFSPAARAGFFRLKFNDSPNCGVVITTGEG
                     GKLSCGEKVVSGYDIFRGVKTYLFLEFERAPRFDNITPGDQGTGAIGSKSIVASCTEN
                     GNGEMRIRYGISYISEEQARKNLEAEIGEWDIDKVEKRARDIWNKTLSVIEVEGGTDD
                     QRHVFYTSLYRCFERMVDITEDGRYFSVWSGQIESAGDTAFYTDDWVWDTYLAMHPLQ
                     VILNPGTQDEKITSYIRMARQSGWMPTFPTPTGDHHAMNGNHSAAVILDAWNKGIRGF
                     DLEEAYRHLKHTVLNETKLPWIRGKATRLDRFYDENGYFPGLLKGETEPYSIVNSFER
                     RQSVAVTLASAYDDWCLAQMAKELGRKDDYDFFIKRSLNYRNLFNTETGFYHPKDSLG
                     RWIEPFDYRFDSGTGARDYYDENNAWTYIWQPYHAFDDLIRMMGGKEKLGDKLDQLFS
                     EGLGRTKWIYYSTFPDATGNVGQFVMGNEPSMHIPYLYNLAGTPWKAQKRLRMLMDTW
                     FRNDLMGVPGDEDGGGLSAFYVFSAMGFYPIAPGIPEYQIGSPLFKKIRIKLQNGRTF
                     NVIAENNSQANKYVQSAWLNGKELGKTSFTHSDITEGGTLILKMGDKPDYRWGVE"
     gene            complement(7272..7949)
                     /locus_tag="PK288_04050"
     CDS             complement(7272..7949)
                     /locus_tag="PK288_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPM18124.1"
                     /translation="MKTKLILLAVILTGIASPSFSQTRPRPDMGIHPAVQAGGYLQTI
                     YGKDFEGKDLNYDLNPGFRAGVNFNIPIAPDLYIQPGLGLALKGFSQEIIAGKRMKVS
                     LYYAEIPVNFLFRPQVGNGHMLLGIGPYAGYGVTGSKRYGKDNDKIKFKSKVSISDLS
                     SAGAFIKPLDAGAGVLFGYELYSGLYFLIDGQLGLLNIYPEYEGLTGNKAAFRNFGFG
                     LSAGFRF"
     gene            8605..9660
                     /locus_tag="PK288_04055"
     CDS             8605..9660
                     /locus_tag="PK288_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18125.1"
                     /translation="MKKFIFLSVLISFYLSLPLHSQSLLNKIKKDVTKEITGKSGNDD
                     SKVAPEPSCSCDNAKLIIDLKKYNLDYRELYLTIGIDGSIVIKDRQTQKFYILKNGQT
                     TGPLNENDPRLIAAGNTSNSNYNDDKEDYYVKAYPGIIVKSGDKYLIKHNGKSYGPYA
                     IISDFALSRSKNKFAAFVTETVLMGESDFKKLEEAMANAKTDQEMMEIGMKMGMQMQN
                     QMMQSGGPESLQSKFVTNIPVSSYDDKKMVPGRLNGTAKYDDIVIQTFDKIFDLKGNP
                     ILTLNGDHYDNDDLFISSTNNKYAYYSSGTLSFNDNTKLSGLFNPYMTSIDGKPSLTY
                     MYHSPGKDAIMQCTIPF"
     gene            9718..10413
                     /locus_tag="PK288_04060"
     CDS             9718..10413
                     /locus_tag="PK288_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18126.1"
                     /translation="MKVLQAVVILGFSTCILFSQEQDYRNTFGKDWDKAAEFINENEP
                     WMKKLSARFNVSYPVAVAVVFPELIRYSALRDKMEITILKSLYIYAGEDYANFSVGRF
                     QMKPSFAEAVNERSHLLKGSLRNFFPDPAAYNTAWRYRSSIVANLENPQAQFIYLIAF
                     IRICGMTFDMGGMDEEEKVKFLSAAYNYSFLKSFEEVNLMADRKFFSTKLVASEYYSY
                     SDISLAWFRSFNR"
     gene            10966..11430
                     /gene="lysM"
                     /locus_tag="PK288_04065"
     CDS             10966..11430
                     /gene="lysM"
                     /locus_tag="PK288_04065"
                     /inference="COORDINATES: protein motif:HMM:NF008399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HPM18127.1"
                     /translation="MGLISFIKNAGDKLFKSEEKREQEKADLIVAHIKKFGFDTSDIK
                     VKVDDDKVILMGSIDTIENKNKVIVTAGNVDGIATVDDWLVVKNPPVVTPPPPEKQYY
                     TVKKGDYLSKIAKEVYGNANKYNIIFEANRPMLKDPNLIYPGQVLVIPPLDK"
     gene            complement(11803..12198)
                     /locus_tag="PK288_04070"
     CDS             complement(11803..12198)
                     /locus_tag="PK288_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18128.1"
                     /translation="MEENTTHESGQTEKERLIDKAKKLVDKADDFIDDKVEDLKKSET
                     FKKVKETYEKAENFVEDKIEDIKQSGVKEKLGAFAGKVEDKTGETVAKMKKFGKKAAE
                     KTAEKLEDIAENLKKKAGGNDMEKKPDNA"
     gene            12347..12919
                     /locus_tag="PK288_04075"
     CDS             12347..12919
                     /locus_tag="PK288_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPM18129.1"
                     /translation="MEKEIITAAAIGLGLAASCGFRVFVPLLVASVAAKLGIFPAAEG
                     FQWLAGWPAIICFGTATVVEVMAYYIPFVDNLLDTITTPLAIAGGTLLMTSVLPVDND
                     LLRWAAGLIFGGGSAAAVQGGTVVTRAASSKLTAGTGNAVLATGEHAASFGVSVLALF
                     IPLIIAALLIVLIVYLISRSGRRIRGGKKP"
     gene            13023..13445
                     /locus_tag="PK288_04080"
     CDS             13023..13445
                     /locus_tag="PK288_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18130.1"
                     /translation="MEAEHNQSKPSRWAGVVKATRRIVRWTVFILLLALGSFIYFKYF
                     FTYSEGSRAGLLQIFSKRGVFFKTYEGELILSSVSSNSNVALASEKFQFSVLKKHMTP
                     EFDTLQGKNVIVYYIEKKGAIPWRGESRFLVDSIALRK"
     gene            complement(13523..15079)
                     /locus_tag="PK288_04085"
     CDS             complement(13523..15079)
                     /locus_tag="PK288_04085"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HPM18131.1"
                     /translation="MERKETFAAHINSGYACKGDSVILGGAMLDGVTITGAHVKVPLR
                     TMNRHGLIAGATGTGKTKSLQVIAEQLSQKGVPVLVMDIKGDLSGIAMPGTEKPFITE
                     RHEKIGLPYSVRGCPVELLTISKQDGVRLRATVSEFGPVLLSRILDLNDTQGGVLAVI
                     FKYSDDRGLPLLDLKDLKKLIQYATGEGKAEFEQDYGKISASSTGIILRKIMELEQQD
                     ADLFFGELSFEVRDLMRRDRDGNGYINIIRLTDIQNRPKLFSTFMLCLLAEVYATMPE
                     LGDVDKPELVIFIDEAHLVFNQASKALLNEIENIVKLIRSKGVGVFFVTQNPMDVPNN
                     VLAQLGLKVQHALRAFTALDRQAIKLTAQNFPLSEFYKTDETLTSLGIGEALITALNE
                     KGIPTPLAAVMMRAPMSRMDVLTQSEIDSINRNSELVRKYYQTIDRESAFEILTRKIE
                     TAQKQAAAETERKEWEKQRERERREETGRRRTTGTTVRRSTARSPVVKVLTSATFIRG
                     VFGIMNKILR"
     gene            15348..15683
                     /locus_tag="PK288_04090"
     CDS             15348..15683
                     /locus_tag="PK288_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HPM18132.1"
                     /translation="MAKFEIYQSGQKNEFRFRLKADNGQTILSSEGYTTKASCLNGIE
                     SVKKNASDPKRFTKTTTPTKMFRFSMTAANNQIIGTSQNYKSESGRDNGIESVKRNAP
                     KAEIKEVDK"
     gene            16016..16975
                     /locus_tag="PK288_04095"
     CDS             16016..16975
                     /locus_tag="PK288_04095"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPM18133.1"
                     /translation="MIKKSGITLFISVSLAVCVSGQVTDAEKHLRAVSADTTLGWKKG
                     GIFSVTLSQTALKNWAAGGQNSVAVNGIFSLFANLRGEKSSWDNSLDLGYGIMKQGKN
                     EDFRKTDDKIDFLSKYGRQAFGKFNYAALLNFKTQMQPGYNYIDNDNKNKISDFLSPG
                     YLTLALGLDYKPNDYFSTFIAPFTGKFTFVTDGFLSDQGAFGVKPGENTRGELGGYLR
                     MIYSRNDFTNEILKNVAFTTKIDLFSNYLDKPQNVDVSWETLIALKVNKFVTVNLNTH
                     LLYDDDIKVPFDKNGDGIITENEVVGSKVQFKEIFGVGLSYKF"
     gene            16999..17406
                     /gene="mscL"
                     /locus_tag="PK288_04100"
     CDS             16999..17406
                     /gene="mscL"
                     /locus_tag="PK288_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPM18134.1"
                     /translation="MKIFDEFKAFAIKGNMIDMAVGIIIGAAFGKVVSSLVSDIIMPP
                     IGLLLGGVNFTDLKLVMKAATDTAEAVTWNYGSFIQVVIDFLIVAWAIFIVIKAINAF
                     KKKEEAAPAAPAALPEPTKDQALLTEIRDLLKK"
     gene            17649..20432
                     /locus_tag="PK288_04105"
     CDS             17649..20432
                     /locus_tag="PK288_04105"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPM18135.1"
                     /translation="MKRTVCLFLISILISVSCSHDIHSGIRIGNLKCNNLTDPAGIDS
                     IPSFSWIPESVTRGAGQSAYQIILDRNINRLKQERTCLWNSGKIVSPETAWIVYSGPP
                     LEPSEKYFWKVRIWDQDDAVTEWSDAAFFITGLFADMNWKGAEWIANEVLPDSLLLVP
                     GVHGNGDDLGNIALKRTVIPYFRKEFNTGKKISQAYVFISGLGHYELHINGRKIGDRF
                     LSPGWTNYDKTCLYNTYDVTKELKQGTNALGVIVGNGFYNINRERYRKLVIAYDAPKM
                     IMALRIRYSDGSAETIVSDGSWKTAPSPITYSGIFGGEDYDARFEQDGWSEPGFDDSG
                     WNSVLTARRPKGVLRPEADYPLRVMEAFDDAQATRLSDTMFLYDFGQNASGIVKLKVK
                     GVKGRTVKLIPGELIDDRSNVTQKASGFPYFFTYTLKGSGEEIWSPRFTYYGFRYVQV
                     EGAVPAGNPNPHDLPVITGLELLHTRNSSPPAGSFNCSDSLFNSIYKLISWAIKSNLA
                     SVATDCPHREKLGWLEQTHLMGNSIRYNFDIRNLYSKIIDDMAGAQLENGLVPDIAPE
                     YVPFYAGFRDSPEWGSACTILPWQMYEWYGDLGAVKKAYPMMKKYVGYLESRADRYLL
                     SHGLGDWYDLGPKFPGEAQLTPKALTATSIWYHDVDLLARMAGLTGNTDDQAHYRKLA
                     GNIREAFNKEFFNPETKVYSTGSQTAYSMPLYFGMVDPACIPEVTENLVSSVIAGNKA
                     LTAGDIGYRYLLRVLEQGGHSQLIYEMNSRTDVPGYGYQLSRGATSLTESWAALRDVS
                     NNHMMLGHLMEWFYSGLGGIRQIPGSRACDSIFICPEPVKGIDRAETTYLSIRGEIRT
                     SWKISENTFTLKVKIPMGCRATISLPGTDPGRITEDDTSIFSSSHVKVRGTGNGRVLC
                     EVPSGEYNFKTTF"
     gene            20493..22067
                     /locus_tag="PK288_04110"
     CDS             20493..22067
                     /locus_tag="PK288_04110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPM18136.1"
                     /translation="MSYKGIISKFAIFLSLLLLCYCRNETTPRAAGSLQRSTPEAEGV
                     TSESIIGFIKAVEASPHELHSFMFLRHGKVIAEGWWDPYEPGLKHTLYSASKSFTSTA
                     VGFAVTENLVKVSDKVITFFPEFLPDKVSPFLADMEVRDLLTMSAGQTPDPTRILTMN
                     ETNWVKGFLALPVKDDPGTKFLYNSMATYMLSAIVQKVTGEKLIDYLTPRLFEPLGIS
                     GMDWETDPEGINTGGWGLRLKTEDMARFGQLYLNRGMWNGRQILPAAWINEATGFKID
                     QMPGAVQAVRDSSDWLQGYCYQFWRCRHNAYRGDGAYGQYIIVMPERDAVIAITSETP
                     DMQKELNLVWKFLLPGMKEQKLPENPASYTELKGMLSDLELSPGTEKPDPQVAAMVSG
                     RKYSFAHNDRNLGAISFKFAGDVCNVILTAGETDYFFSFGNGKWIKGETEKKGPNLLE
                     NARGHFAGLGPAKTACSYNWQDENTITLILRYIESPHSETYTCRFEGDSIEVALKISF
                     AFESEPVILRGNAVNN"
     gene            22161..22529
                     /locus_tag="PK288_04115"
     CDS             22161..22529
                     /locus_tag="PK288_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HPM18137.1"
                     /translation="MGRYVNNHLIRDESVVYETRLHWVIFLTLKSLFTLGILPLLQRK
                     LSEFVITNRRVIIKTGFIARSTFEMNLSKIESVNVDQNIPGRIFNYGSITIIGTGGTK
                     ETFNNITRPLNFRKAFQELC"
     gene            complement(22573..23865)
                     /locus_tag="PK288_04120"
     CDS             complement(22573..23865)
                     /locus_tag="PK288_04120"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HPM18138.1"
                     /translation="MKNSVIALLFPLLGFFCVEAAGGSDSAPTGLRAGFAKTDITPEI
                     PVKLYGYAARKTYSEGVHDPLSARAVAFENNGRKLLLVSTDLGSFGRDVFTVFQKSIM
                     DRFNLRESEIFLSAIHNHSAPILTLNPERGDPNNIEYTKGLQKKLLDLIGEAFRNMQP
                     VLTGTGTGASPVGVNRREMLPDGSITLGRNPYGVTDKEVLVLKIAAGDGNSLGALYDY
                     ATHCTSLGGSSMKISGDILGLSSQFVEKILGGKVITPAFAGASGNIDPWFRVLPGFNE
                     EPGWIPEPVLLGTLLGEEVVHVYRGIKELKPGGEIRTSFETLECPRRETAADQAGAAQ
                     PQSPTIPVNITVASIGDDVAFVGFNVEMITEIGLEIKKGSPFRHTFIITHCNGSSGYL
                     VPAEFYKEGGYEVTTTRFAIGSSDMVVKKALKMLYDLH"
     gene            24277..>25605
                     /locus_tag="PK288_04125"
     CDS             24277..>25605
                     /locus_tag="PK288_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18139.1"
                     /translation="MKRFLITSIFAAIVAAGTAMMPQEYPKEYLGLPGDNLNLYAVMK
                     LFQESETLEGFERSLNDENSRINNLDLNNDNYIDYIKVLDYVDGNVHTIVLQAVLGRN
                     ESQDVAVFTVERFRDGSVQIQLVGDEALYGRNYIVEPYYAETPNPGYTGTRVRNNTTV
                     VRTTYVEVAAWPVIRFIYLPGYTVWRSSWYWGYYPVYWRPWRPYYWHYYYGYHYHWFP
                     EYYRYYHRYDRLRWTGWDRYYYRDRRVYSTNVTININTGKYRDTYSRPDQRKAGEAMY
                     AKAHPERAATMSATGSRRSAADASTSRQGSGTSVNQGRRSSGTVSTGRSTSPSQGQAG
                     TSRRSSETVNQNRGTAPVQGQAGTSRRSTETVNQNRSSAPVQGQAGGTRRSGSPAMNS
                     GTRSTPQAGQAATGNRRSPEAAARPAPESRSQASAPRAASSARRGSTGAPA"
BASE COUNT         7200 a         5716 c         6069 g         6620 t
ORIGIN      
        1 tcaaatataa ctaattaagt tgtaaatgaa gaacaaaaac ccccttttta tgaattattc
       61 aggttaaata cttttaacaa tccacggatg aatttttacc ccccccttat ttagatacat
      121 tctaaataag ctgttgaaat catcggaatt gcttctcatg aaacagtcct gaaaagttaa
      181 atatcaaacc gggaattgta ctctgcctgc atccggactc ccggcaataa aaggacaatc
      241 accggtaagg aaaatcataa atcaattgct gaatacttac tgatagaaaa atgattatca
      301 ttgcaataaa ttgttgaaat atcattcagg ggccggtatt acatttctgc ctgccgattt
      361 ataaaaaacc aacaacatga tcatgaacaa actgatagca atcgttcttc tggtttttct
      421 tgcctctttc acccgcggta atgagccgaa aattccctgc tacgcctggc tcgacggccc
      481 gggcaaagct actgacacca ggctgaaggc gcagttcacg gatctcagga gcaaggggat
      541 tgacgggctc atgtacacgg cgggccgaga tccggaggtc taccgccgtg tgggaaagat
      601 cgcaaaagag gccgggctgg aattccatgc atggataccg acgcttatcc agagcaataa
      661 cccggatatg aaacctgaat ggtatgctat caacgggctg ggagagtcgg cttatgacaa
      721 gcctgcctac gccgaccatt ataaatttgt ctgccccaac cggaaagaag ttgttcaata
      781 tcttgaaaac ctgtatggga caattgccgg tatacccgaa gttgacggca ttcacctcga
      841 ctttatccgt tttcccgatg tgatccttgc cagaggtttg tggaaaaagt acaatctggt
      901 aatggacagg gaatatccac agttcgacta ttgttattgc gacaagtgta aaaatgattt
      961 cctgataaac acgggggtcg atctgaatga tgtggaggat ccgtcgcagg ttgaaaaatg
     1021 gaagcagttc aggtacgatc tgataactga cctggtcaac aggctggcag atgcagtgca
     1081 ttcaaagggt aaaaaaatta ctgccgcagt ttttcccggt ccgaattcca ttgcaaaaaa
     1141 gattgtaagg caggaatggg acaaatggaa gatcgatgct atttatccga tgaactacaa
     1201 cgacttctac ctcgagggta cgaaatggat aggtgacatg tgcagggaag gagtcacctc
     1261 tctcaacaat tccaaacctt tgtacagcgg actgtttatc tgtcccgatc cggccggcaa
     1321 ggcgaatgac cctgatcccg agaaccacgg gctggtgcca ggtgaacttg aggctgcgat
     1381 aagggaatcg atggagaacg gcgcggcagg tatctgcctt ttcacccccg acaggatgac
     1441 ttccgaagac tgggtgatat ttaaaaaggt aatccacaat aagtatcaga ataaagtact
     1501 cccggcaaaa tagaagattt tacgatacta tagtccttta atccaaccac agggaacacg
     1561 gttgcagtct gcctgccggc ttactgcagc actgtgctgg cacagattga aagtgcttct
     1621 gaatagtctg atttacttac atgattattc ttcgtatgcc atcttttaat tgctgaccat
     1681 taaagttgat aaatcaatta aaattcatta agaaagaata acagtgttct ccgtgcttcc
     1741 gatatgccgg caggcgtatc ggaacagtgg ttaataagga attcaaattg acagacagaa
     1801 tccggtccgg gagagatttt taaaagctaa gaaatttatt catatcttgc tgattgttaa
     1861 tatccggaat tatacaagtt cctttatcgt attaagtatt tatgcatcag ggtttgtaaa
     1921 tatgcagaga tgggtatttt aaaacgacat gctattttta ttcttctctt gttaattgcc
     1981 tttattcttc ttgcaaacaa gctgttcatc tggggatttg tcgccctggg cattggattg
     2041 ataattttcg gcatccagca gttgatcggg ctgaacgtaa aactgtcgaa gttccaggac
     2101 cgggtcgggg gactggaaga aaaaaacaag tcgctgctta acctgaacag gaaactggag
     2161 gaagaagtca gtttccttaa agagaggcag tttcagataa caaagatcag gaatatcctg
     2221 gaacttaacc tgtttgaagt cgaaacaacc ttcaacaggc cggtttccag gaaggttacc
     2281 tcggaagatg accgggaaat taagtatttc ggcagcctta atatatcact gggagccaag
     2341 ttcggaatag actgcaagga actgagattc aaatatcttc ctgagaatga cgaacttatt
     2401 gtggcaaaca tcaatccccg gttcctttca ttcggaaaga gaaaactgga atgggatttt
     2461 tttgaaatat ttgagttccg cagtcagaat gcacttgccg aaaaaagatg gatgacaacc
     2521 gacaatttgt acaaatatgc cagtaaagta aaggaagaat accggctgaa caccgagaaa
     2581 tcacttgaga agggtccgga ggagctggaa tggatccaca aacctattat gcagactgta
     2641 ataaacacta tcagggcagt tttcggcagc ctctgtaaaa acatcactat tgctgaaact
     2701 gccgatgaga cattcgtgcc aatcgaaaaa ataaggtttg atccattgca ggatgcaaag
     2761 gagattcagc cggcagaaaa aaaagcctta aaagagagct gatctgaaag ggatccggct
     2821 tatttgccat cttctgttaa cagaataaca ataaaacggg aaaaaatttt ttccccggat
     2881 catgaatgct taactgctgc cggactcctg aaccaactaa aagaattgta aatttacttt
     2941 tttcaccatt ctgacggaaa tgccggagac aatcatttca aaccaggggg ctggttctgc
     3001 agatgaagcc ttgtatcccg attattttgc ccggttctat gacctgatat atcatcaggt
     3061 aagggacgat gtggataatg aatattacct gaaaaaggcc gtcgggacga aaggaaggat
     3121 actcgaggca ggtgtcggca cagggcgttt gtttaccgcg acccttgaca acggcgcaga
     3181 catatacgga atcgatatca gcccggccat gacagatatt ctgaaaagca ggctgaatga
     3241 cagccagggg tcaagaataa cctgtcagaa cataattgat tttaaattcg acttcagttt
     3301 tgaccttatc atggctcctt tccgggtatt catgcatctg acggagaaat ctgaccagat
     3361 cagggccctg aacaatgtct atgaacatct gaatccgggc ggcagattta tattcgacgt
     3421 ttttgttcct gatctgcaga tacttatttc cggcctgaac aatgtgatcg atttcgacgg
     3481 ggagtttgaa ccgggaaacc ggttaaaaag gacagtcacc accagacccg acctcattaa
     3541 tcaattgatt aacgtcactt tcagatttga ctggaatgaa ggaaggtcaa ttttcacaaa
     3601 ggaatggaac acttccctga gatatttttt caggtttgag cttgagcatc ttatcgagcg
     3661 gtcagaattc gacagttaca cgatctgcgg cgattttgaa gaaagcgaac tgtctccgga
     3721 gtcgaaggag tttttaatat catgcatgaa atgctgatga aatccacatt attgaccaaa
     3781 aacccaaatc cggatgaagt taatcgtttg caatcatgtg tgtttccctg cccggggggt
     3841 cagacaggca tccgaacttt aaaaataaat catatccggc tgaaaaattt attgctaatt
     3901 ccggttttaa tcatatgttt aagcatgcaa gcccagaata agagagcagc cgactatgga
     3961 atacgcttcg gagtgttgaa tccggggcgc aacaatgcca ttacagatgt acccggagtc
     4021 aaagtgggac atgttaccct taaggaaggc aacgctgtaa acacaggtgt gacagcaatt
     4081 ctgcctcatt caggtaatat ctttcagaac aaggttccgg cagggatctt tgccggcaac
     4141 ggatttggca agctcaccgg cgtatcccag gttgaagagc tgggaaatat agaaaccccg
     4201 gttattctga cgaataccct gagtgttccc gttgcagcag aggcattgat tgattatacc
     4261 ctgggtcttc cgggcaatat taccgtgatg agcgtcaacc ccgttgtcgg ggaaaccaat
     4321 gatgccgggc ttaatgatat ccggagaagg gtcatcacca gggagcatgt cctcaatgcg
     4381 atagctgcag ctgacacagg tccggtggct gaagggaatg tcggagccgg gacgggtacc
     4441 gtctgctttg gattcaaggg aggaatcgga acctcttcga ggattctccc ggaatcaaag
     4501 ggaggctata ccgtgggagt gcttgtccag acaaatttcg gaggcatact caggataaac
     4561 gggataccag taggaattga gctcggcagg cataaattca tgaagattac cgaaaaatac
     4621 aaggaagatg gttcctgcat ggttgtaatc atgaccgacg cccctctcga ttcaaggaat
     4681 cttaaaagac tggcggcaag ggctatggga gggatcgcaa tgaccggagg catgacttcg
     4741 aacagcagcg gggattttgc aattgccgta tcaactgcgg aaaacctgag gatcccctgg
     4801 agtgcgaaat caatgtacca ggaaatgaca gttcttagaa atgaagagat ggatcttctt
     4861 ttcgaagcag taattgaatc gaccgaagaa gcgatcataa attctctttt tgcagctgaa
     4921 acagtgaccg gccggtataa tttcaggatt gaagcgctgc cggttgaaaa gacacttgag
     4981 atcctaagga aacataacat cataaaataa cctaatacct cagcatatga acaggatacc
     5041 cgggctttgt actgtaattg ttactgccat actgatttca tcgtccattt catgcagtca
     5101 gaagacacct gatccggttg attacgtcaa cccgtacatg ggaaacatca gtcatctgct
     5161 ggtacccacc taccctaccg ttcatcttcc gaacagtatg ctgaggtttt atcccgaaag
     5221 ggataattac acaaccaaca aaatggcggg atttcccctg aatgtagtat cgcaccggtc
     5281 aggaacagtt ttcatgatca ttcccttttc ggggagtaat gccggtgagc aggctttaac
     5341 cccttttaca actgacaatg aaaaaatcag tccttaccgt tattctgtaa cgcttgatga
     5401 aagaaacatc ggtctggaat tctcccctgc agccagggca ggttttttca ggctaaaatt
     5461 caacgacagc cccaattgcg gggttgtaat cacgacaggc gaaggaggta agctcagctg
     5521 cggggaaaag gttgtttccg gttatgacat tttcagggga gtgaagacct atcttttcct
     5581 cgagttcgag cgggctcccc ggtttgataa cattaccccg ggggaccagg gaacaggggc
     5641 catcggcagc aaatccatag ttgcatcatg caccgaaaac ggcaacggcg agatgaggat
     5701 tcgttacgga atatcctaca tcagtgagga gcaggcccgt aaaaatcttg aggcagagat
     5761 cggcgaatgg gacattgaca aagtggaaaa gcgggcgcgt gacatctgga acaaaactct
     5821 ttcggttatt gaggttgaag gaggaacaga tgaccagcgg catgtcttct acacatcact
     5881 gtaccgttgc tttgaaagga tggtcgacat caccgaagac gggaggtatt tcagtgtctg
     5941 gagtggccag atcgaatctg ccggtgacac ggccttctat accgacgact gggtgtggga
     6001 tacatacctt gccatgcacc cgctgcaggt tattctcaac cccggcacgc aggacgaaaa
     6061 gatcacatcc tacataagga tggcaagaca gtcgggatgg atgcctacat ttcccacgcc
     6121 gacaggagat catcatgcaa tgaacgggaa ccattctgcg gcggtaatac tggatgcatg
     6181 gaacaagggg ataaggggat ttgatcttga agaggcatac aggcatctga aacacactgt
     6241 tctcaatgaa acaaagctgc catggatcag gggaaaggca accaggctcg acaggtttta
     6301 cgatgagaat ggctactttc ccgggcttct caagggagag accgagccct attccattgt
     6361 caactctttt gagcggaggc agtcggttgc agtaaccctt gcctcggcat atgatgactg
     6421 gtgcctggct caaatggcca aagaactggg gaggaaagat gattatgatt ttttcattaa
     6481 gcggagcctc aattacagga acctgttcaa taccgaaacc ggattctatc atccaaaaga
     6541 ttccctggga aggtggatcg agcccttcga ttaccgcttt gattccggga caggggcgcg
     6601 tgattactat gatgaaaata atgcatggac ttatatctgg cagccctacc atgcttttga
     6661 cgatctgatc cgcatgatgg ggggcaagga aaaactcggg gataagctcg accagctatt
     6721 cagcgaaggg ctgggcagga ccaaatggat ctattattca acctttcccg atgccacggg
     6781 caacgtagga cagtttgtta tgggaaacga gccgtcaatg catataccgt atctatacaa
     6841 cctggccggt acgccctgga aagcgcagaa acgcctgcgg atgcttatgg acacctggtt
     6901 caggaatgat ctcatgggag tgccgggaga tgaggacggc ggcggattat ccgctttcta
     6961 tgtgttctcg gcaatgggtt tttatcccat agcgccaggg atccccgaat atcagatcgg
     7021 tagtccgcta ttcaaaaaga taagaattaa acttcagaac ggcaggacat tcaacgttat
     7081 tgctgagaac aacagccagg cgaataagta tgtgcaatcg gcatggctga acggaaagga
     7141 gctgggaaaa acctctttca cgcacagtga tattacggaa ggcggaaccc ttattcttaa
     7201 aatgggtgat aagccggatt accggtgggg tgttgaatga aaccaggtgt tcgttttttc
     7261 agcagctgtt ttcagaatcg gaagcctgct gacaatccga aaccgaaatt cctgaatgcg
     7321 gctttgttgc cggtcaggcc ttcgtactca ggataaatat tcagcaggcc caactggcca
     7381 tcgatcagaa aatacaatcc gctgtacaat tcataaccga aaaggacacc tgctcctgcg
     7441 tcaaggggtt tgataaaggc acctgcagac gacaggtcgg atattgatac cttgcttttg
     7501 aattttattt tgtcgttatc tttcccgtaa cgcttgcttc cggtaacacc gtaaccggca
     7561 tacggaccga tcccaagcag catgtgcccg ttacccacct gcggccggaa aagaaaatta
     7621 acgggaatct cagcataata caggcttact ttcatccttt ttcctgctat gatttcctgg
     7681 ctgaagcctt taagtgccag tcccagtccc ggctgaatgt acaaatcagg tgcgattgga
     7741 atattgaagt taacgcctgc gcggaaaccc ggattcagat cataattcag atccttcccc
     7801 tcaaaatctt taccgtagat ggtctgaaga tatccgcctg cctgtactgc gggatgaatt
     7861 cccatatcgg gtctgggtcg tgtctgagaa aatgacggtg aggcaatacc cgttaaaatc
     7921 actgccagca ggatcagttt tgtcttcata ttttaatttt tgtttttact cagttatata
     7981 attttccata aaagggattc actaagcccg gccaaacagg ctctggaatt ccatgttttt
     8041 ttgaactaca ataattgcag atttgcatca tgcttagtat ataacagata atctgcaggt
     8101 tgatgtctga aatatatgac tgccgctgca tctgaattcc ggttttcccg gtaaatctga
     8161 ctgttcattt tcaccggcaa tttattgtgt ttttgccata tgatgaactt tgcaaaaagt
     8221 tacttttcaa attatcatct gaattattaa caaaatgcca gcataaaaag ttccctgaaa
     8281 ccatggctgg tccgggaaaa gccgggagaa ggctatgggg tgaaacttct ggttattaaa
     8341 aaaagacgtt tttttgttgt aactttcttt tgcttctcca aaagaaagtc accaaagaaa
     8401 aagagccact gcgccgctgt attcaggata ctgccatttt ttgttttggg cggagttcag
     8461 tgaaaataat tagatgattt aatttcagaa aaaacagatt gccgttgaac acttattctt
     8521 ccatagtgtt atatcacaaa tttcacatcg tttaagaaaa aagaagatta tttcctgata
     8581 ataacaacaa taaatttagt atctatgaag aagttcatct ttctctcagt tctgatatca
     8641 ttttacctgt cattgcccct ccattcacaa agcctgctta ataagataaa gaaggacgtg
     8701 acaaaagaga tcaccgggaa aagcggcaat gatgacagca aggttgcgcc tgaaccttca
     8761 tgcagctgcg ataatgcaaa gctcatcatc gacctgaaga aatataatct cgattacagg
     8821 gaattgtact taaccatagg catcgacggc agtatagtaa taaaggacag gcagacacag
     8881 aaattctaca tacttaaaaa cggacagact actgggcctc ttaatgaaaa tgatccgcgg
     8941 cttatagctg ccggcaatac cagcaattca aattacaacg acgataaaga agactattac
     9001 gtgaaggcct atcccgggat catagtaaaa tcaggcgata aatatttaat aaaacacaat
     9061 gggaaaagct atggtcctta cgctattatc agcgatttcg ccctgagcag atcaaaaaac
     9121 aagttcgcgg ccttcgtcac cgagaccgtg ttaatgggcg aatcggactt taaaaagtta
     9181 gaagaggcaa tggcaaatgc aaaaaccgat caggaaatga tggaaatcgg tatgaagatg
     9241 ggtatgcaga tgcagaacca gatgatgcag tcaggcgggc cggaatcatt acaatcaaaa
     9301 ttcgttacca atatcccggt atcaagttat gatgataaga aaatggttcc cggaagactc
     9361 aatggcaccg ctaaatatga cgatatcgtg atccaaacct tcgacaaaat atttgacctg
     9421 aaaggaaatc caatcctgac acttaacggg gaccattatg ataatgatga tctttttata
     9481 agcagtacga acaacaagta tgcatactac tcatcaggga ccctgtcttt caatgataat
     9541 acaaaattat cgggcttgtt taatccttat atgaccagta ttgatggaaa accaagcctc
     9601 acttacatgt atcattcacc ggggaaagat gccatcatgc agtgcacgat tccattctga
     9661 ccctcatatt aataaccgcc ggttattcag acggaccatt caatttcagg ttaccggatg
     9721 aaagttcttc aggctgttgt tatactgggc tttagcacat gtatcctgtt ttcccaggaa
     9781 caggactaca ggaatacttt cgggaaggac tgggacaaag ctgcagaatt cataaatgaa
     9841 aatgaaccgt ggatgaaaaa gctgtcggca aggttcaatg tatcttaccc ggtagctgtt
     9901 gctgtggttt tccccgaact gatacgctat tccgccctcc gtgacaaaat ggaaattaca
     9961 atccttaagt cattgtatat ttatgccggg gaggattatg cgaatttctc cgtcggccgt
    10021 ttccagatga aaccctcatt tgctgaagct gtcaacgaaa ggagccattt attgaaaggc
    10081 agtctgagaa actttttccc tgatcctgca gcatacaaca ccgcctggag atacagatcg
    10141 tcaatagtgg ccaatctcga aaacccccag gcgcagttta tctatcttat tgcctttatc
    10201 aggatatgcg ggatgacatt tgacatggga gggatggatg aagaggagaa ggtaaaattc
    10261 ctgtcggcag catacaatta cagctttctg aaaagctttg aggaagtgaa cttaatggcc
    10321 gacaggaagt ttttcagcac gaaactggtt gcatcagaat attattccta ttctgacatt
    10381 tcactggcct ggttcaggtc atttaaccgg taataagagg taatagcaac cggggttagc
    10441 ctggacctgc atcgccccag tattggccgt aaacggccgg tttggttcgg ggtcaggaga
    10501 tatccgtttc tgattaaaat ccgggcgccg gttaagggcc catggtaaac ccggtcccgg
    10561 gttaacaacg catgaaggcc gacaggatga catagactaa aatgcaggtc ttatatttca
    10621 atttccgggc ataactaaac atccccggga agtatatttt aatcaaccat cttaaaaatt
    10681 attctgaaaa agaagcattt gtttattgaa acagaatgat taatttggga tcaataaaga
    10741 gttttcttca gtcaactgat ttttatgtct gtattattta aaaatataca tgttatacag
    10801 ccatgtcgat acgccggaca ccggtggtct gcttgtttca tcatatgcgc attttagaag
    10861 tgccgcagtg aacacagaca ttggaacatg aaattccggg acctgacagg ctggttcaga
    10921 tgaaaggcat ataaaagaat caatttcaaa ctaaaactgt taactatggg actaatttca
    10981 tttatcaaaa acgccggcga caagcttttc aagtcagaag aaaaaaggga acaggaaaaa
    11041 gctgatctga tagttgctca cattaagaag ttcgggtttg acacgagcga cataaaggta
    11101 aaggtcgatg acgacaaggt tatcctgatg ggcagcatcg acacgatcga gaacaagaac
    11161 aaggtcatag ttacggccgg taacgttgac ggcattgcca cagttgatga ctggctggtt
    11221 gtaaagaatc cgccggttgt caccccccct ccgccggaga agcagtatta caccgtgaag
    11281 aaaggtgatt atctttccaa gatcgcaaag gaagtctatg ggaacgcaaa caagtacaat
    11341 atcattttcg aggcaaacag gcctatgctc aaggatccta acctcatcta cccggggcag
    11401 gtgctggtta ttccgcctct tgacaaataa gttaccagaa taattaccgt aattataaaa
    11461 tgatttttta ttttgagggt gtcctggaag gatgccctcc ctttttcagg aaagcagttt
    11521 attccttgtg caccacaaaa atagtgtaac aggaaaagca gcggggaaaa gaactcattc
    11581 tccggtttca cactgattca gacaacaata tccattttta cctgcaaaat ccggcaaaac
    11641 aacggagata taccaaacgc atcattttta cccctccggc cagcgataag gaggcaaacc
    11701 ccggcggtct ccgctgactg gttgcaggat ttgttcatgt accatcatag ggttacgtaa
    11761 gggaaacagg aatgctttcc cccgtttgaa tagccgcgct gatcaggcat tatcaggttt
    11821 cttttccata tcattgcctc ccgctttctt tttcaggttt tcagcaatat cttcaagctt
    11881 ttcggcggtt ttttcagcgg ctttcttacc gaatttcttc atttttgcga ccgtctcccc
    11941 tgttttatcc tcaacctttc cggcaaatgc cccgagtttt tcctttacgc cgctctgttt
    12001 gatgtcctca atcttgtctt ccacgaaatt ctccgccttc tcataggttt ccttcacttt
    12061 tttgaaggtt tcactttttt tcagatcttc gactttatca tcaataaagt catcagcctt
    12121 gtcaaccagc ttttttgcct tgtcgatcag tctttccttt tcagtctgac cggattcatg
    12181 agtcgtattt tcttccattt gatgaattat taggagagcc attaataagc atgtttccga
    12241 acggattgtt taatttccat gaaagttact aaaagaataa atatcctgat ttccggatat
    12301 gaattttttc aggaatccgg catatcttta gagaaatgtt cgggtcatgg aaaaggagat
    12361 catcacagca gccgcgattg gccttggttt ggcggcaagc tgcggattca gggtgtttgt
    12421 gcccctgctt gttgccagcg ttgcggcaaa gcttggtatt tttcctgcag ctgaaggctt
    12481 tcagtggctt gccggctggc cggccataat atgtttcgga actgcaacgg ttgttgaagt
    12541 catggcatat tacatccctt ttgttgacaa tctgctcgat acgataacca ccccgctggc
    12601 gattgctggc gggactctcc ttatgacctc ggttttgccg gttgacaatg atctgctcag
    12661 gtgggctgcc gggcttattt tcggaggggg atcggcagca gccgtacagg gcggaacggt
    12721 agtgacccgg gccgcttcat ccaaactaac cgccggaaca ggtaatgcag tgctggcaac
    12781 cggggaacat gctgcatcgt tcggagtttc tgtactggcc ctgttcatac ccctgatcat
    12841 tgcagccttg ctgattgtac tcattgtata tttaatttcc cgatcaggcc gcaggatccg
    12901 cggcgggaaa aagccgtaat tatttatccg ttttgcctga ggcggaaaat ggcttgattt
    12961 ttatatgtaa ttttgcatta tgccggaaac tttcaagcct ttataattta atccgtatca
    13021 gaatggaagc agaacacaat cagtcaaaac cctcccggtg ggcaggtgtt gtcaaggcaa
    13081 caagaagaat tgtcagatgg acagtgttca ttcttctcct tgcccttgga tcctttattt
    13141 atttcaaata tttcttcact tacagtgaag gctccagggc aggtttgctg cagattttct
    13201 caaagagagg ggtgtttttt aaaacttacg agggagagct gatattgagc agtgtatcga
    13261 gtaactcaaa tgttgcgctc gcatcagaaa aattccagtt ttcggtactg aaaaaacaca
    13321 tgacacccga atttgacacg ctgcagggaa aaaatgtaat tgtttactac atagagaaaa
    13381 aaggagcgat tccctggagg ggagaatccc gcttccttgt cgacagcatt gccctgagga
    13441 aataaaatcg gccttaaagc ggttcatcca tcctttcagg gatattttcc ttatcctgaa
    13501 aaagatcttg cttccgcggg gatcagcgaa gtatcttatt cattatacca aaaactcccc
    13561 tgatgaatgt ggcacttgta agcactttca cgacggggct tctggcggtg cttctgcgca
    13621 cagtggttcc tgttgtcctt cttcttcctg tttcttctct tctttccctc tccctctgct
    13681 tctcccattc tttcctttcc gtttcggcag ctgcctgttt ctgggctgtc tcgattttcc
    13741 ttgtaagaat ctcgaaagcg ctttcgcggt caatagtctg gtaatatttc ctcacaagtt
    13801 cggaattccg gtttatggaa tcaatttccg attgcgttag aacatccatc ctgctcattg
    13861 gtgccctcat catcactgcg gcaaggggtg tgggtattcc tttttcatta agagcggtaa
    13921 taagggcttc accgataccg agcgaggtaa gggtttcatc cgttttatag aattccgata
    13981 agggaaagtt ctgggccgta agtttgattg cctgtctgtc gagtgcagta aatgccctga
    14041 gtgcatgctg taccttaagt ccgagctgtg caagaacatt gtttggcaca tccatggggt
    14101 tttgggtcac aaaaaagacc ccaacccctt ttgacctgat cagtttgaca atattctcaa
    14161 tctcgtttaa aagtgcttta cttgcctggt tgaacacaag gtgggcctca tcgataaaaa
    14221 tgaccaattc cggtttatca acatcaccca gttccggcat tgtggcatac acctcggcaa
    14281 gaaggcagag catgaatgta gagaagagtt tcggcctgtt ctggatatcg gtgagcctga
    14341 taatgttgat atatccgttt ccatccctgt cgcggcgcat caggtctctc acctcgaatg
    14401 aaagctcccc gaaaaagagg tcagcatcct gttgttccag ttccattatt ttccggagga
    14461 ttatacctgt tgaggaagct gatatcttgc cgtaatcctg ctcaaattcg gcttttcctt
    14521 ccccggtggc gtattgaatg agctttttca ggtccttaag gtcaagcagg ggtaatcccc
    14581 tgtcgtcgct atatttaaag atcacggcaa gaactcctcc ctgggtgtca ttcaggtcga
    14641 gtattctaga aaggaggacc ggtccgaact ccgagacggt tgcacgaagg cgcacaccat
    14701 cctgttttga gattgtaagg agttcaacgg ggcatccccg caccgaatat ggcaatccga
    14761 tcttttcgtg ccgctctgta atgaacggct tctcggtgcc gggcattgct atcccgctca
    14821 gatcgccttt tatgtccatc accaggaccg ggactccctt ttgtgaaagc tgctcggcta
    14881 tcacctggag tgatttggtt ttgccggttc ccgttgcacc tgcaataagg ccgtgcctgt
    14941 tcattgttct tagcggaacc ttcacatgag ccccggttat ggtgacgccg tcaagcatgg
    15001 cgcccccgag gatcacagaa tcccccttgc atgcatatcc actgtttata tgtgcggcaa
    15061 atgtctcttt tctttccatt ttgatttttc tgattttctt taacccggct gaagaccgat
    15121 gcaatttagc agtttagatt taataatcaa aaatttgaaa cagaacatat ttaaacaatt
    15181 atatataata atttgttaaa atttattatc tttgcccgat gatcgctgaa gggtgcctgt
    15241 tgttactgac agaaacggga ggaaaatcag gtaaagtatt tataaaacaa cgaataaagc
    15301 aattaaatta tttaaggtta catctttttt aatcaaaatt caacattatg gcaaaatttg
    15361 aaatttacca gagtggtcag aaaaatgaat tccgtttccg tctgaaagcc gacaacggac
    15421 agacaatcct gagcagtgaa ggttatacaa caaaggcatc ctgcctcaat ggaattgaat
    15481 cagtaaaaaa gaatgcatct gatccaaaaa ggttcaccaa aacaaccacg cctaccaaga
    15541 tgtttcgttt cagcatgact gcagccaaca atcagatcat agggaccagc cagaactata
    15601 aaagtgaaag cggccgcgac aatggtatcg aatcggtgaa gagaaatgca ccaaaggctg
    15661 aaatcaaaga ggttgacaaa taatcatttt tcgctaccgg tcaggaaaaa ccgtttccgg
    15721 tctgcctgaa aagtccatat ccggaagtgt tccccggatt cagcggatac agttgatcca
    15781 aggtcatacc gcaggtactt ttatgcaggg gcggggtatc ctgtacccat tgattttctt
    15841 acggtcataa ggcagcagtc atatttatct gcgaggtgcc cgcgatggtt tttttgtttc
    15901 ggctgtatta atcaaaaccg gaacagtaat caatatataa ttttatctgc tgttacagaa
    15961 tatcaattat gtgctgcagg aaggattgtt aaagttgttc ataaacatat caggcatgat
    16021 caaaaaatca ggaattaccc tttttatttc agtaagtctg gcagtctgtg tttccggtca
    16081 ggtaacggac gcagaaaaac atctccgggc agtcagtgcc gacacaaccc ttggctggaa
    16141 aaaaggaggt attttttcag tgaccttgtc gcaaacggcc cttaagaact gggcagcagg
    16201 aggtcagaat tcagtggctg taaatggtat tttcagtctc ttcgcgaatc tcaggggtga
    16261 aaaatcatca tgggataatt cactcgatct gggttacgga attatgaaac agggtaagaa
    16321 tgaagatttc aggaagactg atgataaaat tgactttctg tcgaaatatg gccggcaggc
    16381 attcggaaaa ttcaattatg ccgccctttt gaatttcaag acccagatgc agccgggata
    16441 taactacatc gacaatgata acaaaaacaa gatctcggat ttcctctcac ccggctacct
    16501 gaccctggca ctggggcttg attataagcc caatgattat ttcagcacat tcattgcgcc
    16561 gtttacgggg aaattcactt ttgttaccga tggatttctt tccgatcagg gtgctttcgg
    16621 ggtgaagccg ggtgaaaata ccaggggcga gcttggtggt tacctgagaa tgatatattc
    16681 aagaaacgat ttcacaaatg aaatcctgaa gaatgttgca ttcaccacga aaatcgatct
    16741 tttctcgaat tacctcgaca agcctcaaaa tgtcgatgtg agctgggaga cactgatcgc
    16801 actgaaagta aataaatttg ttaccgtgaa cctaaacacg cacctgcttt acgatgatga
    16861 cataaaggtg cctttcgaca aaaacgggga tggcataatc acagaaaacg aagtcgttgg
    16921 ttcaaaagtt cagttcaagg aaattttcgg cgtaggatta tcctataaat tttaatcaca
    16981 caactaaaac ctgacaagat gaaaattttt gacgaattca aggcatttgc cattaagggc
    17041 aatatgatcg acatggccgt tggtataatc attggagctg cattcgggaa ggttgtaagc
    17101 tcactggtaa gcgacataat tatgcctccc atcgggttgc tgctgggcgg cgttaatttc
    17161 accgatctga aactggtaat gaaggcagcc accgataccg cagaggccgt aacctggaac
    17221 tatggttctt tcatacaggt ggtaattgat ttcctcatcg ttgcatgggc catattcatt
    17281 gtgataaagg ccatcaacgc atttaaaaag aaggaggaag cagctccggc cgctccggcc
    17341 gctctgcccg aacctactaa ggatcaggcg ctccttacag agatccgtga cctgttgaag
    17401 aaataatccg acagtatttt cccaataccc agcagcatgc attaaaagcc ccggtttctt
    17461 ttaatttgca ggctgcaaat ctctgccgtc cggaatgcga agccccgagc gttattcatt
    17521 gtactgtgga gcttcgcatt ctcatgtacc gggtatgctg accatacccc cataatgttt
    17581 ttttacaatc cctttttatc atgacaggtc agcattatca ggtttaaaac tgtaacttta
    17641 ccttcataat gaaaagaact gtctgcctgt ttctgatttc aattcttata tcagtatcat
    17701 gttcccatga tattcattcc ggcatcagga tcgggaacct gaaatgcaat aacctgaccg
    17761 acccggctgg catcgacagt atcccttcat tcagctggat acctgagtct gttaccaggg
    17821 gagccggtca gagtgcctat cagataatcc ttgaccgaaa cataaacagg ctgaaacagg
    17881 aaaggacctg tctctggaac tccggaaaaa tcgtttcacc tgaaacggca tggatagttt
    17941 attcaggccc cccgcttgag ccttcggaaa aatatttctg gaaggtgcgt atctgggacc
    18001 aggatgatgc tgtgacggaa tggagcgatg cagctttttt cataaccgga ctctttgcag
    18061 acatgaactg gaaaggggca gaatggatag caaacgaggt tctgcctgat tcattgcttc
    18121 ttgtacctgg agttcacggc aacggggacg acctcggcaa tattgccctg aaaagaacgg
    18181 tcatcccgta cttcagaaag gaattcaata cagggaaaaa gatatcgcag gcttatgttt
    18241 tcataagcgg gctcggccat tatgagcttc atatcaacgg caggaagatc ggcgaccgct
    18301 tcctttcgcc cggatggacc aactacgaca aaacatgcct gtacaacaca tacgatgtga
    18361 caaaagagct gaagcaaggt acaaatgcgt taggagttat agtgggtaac ggattctaca
    18421 atattaaccg cgagcgttac aggaaactgg ttatagccta tgatgccccg aagatgatca
    18481 tggccctcag aataaggtac agtgatggca gtgcagaaac gatagtgtcg gatggaagct
    18541 ggaaaacggc cccctctccc attacatatt caggcatttt cggaggagag gattatgatg
    18601 cacgttttga gcaggacgga tggagtgaac ccggctttga tgattcggga tggaacagtg
    18661 tactgacagc caggaggccc aaaggggttc tccggcccga agccgattat ccgctcaggg
    18721 taatggaggc gtttgacgat gcacaggcaa ccaggctcag cgatacaatg ttcctttacg
    18781 atttcggaca gaacgcgtcg ggtattgtaa aactaaaggt taagggagta aagggcagga
    18841 ccgtaaagct catacccgga gagcttatcg atgacagatc caatgtcacc caaaaagctt
    18901 caggattccc ttacttcttt acctataccc ttaaagggtc gggagaggaa atttggagtc
    18961 cgcggtttac ctattacggt ttcaggtatg tacaggttga aggggccgtt ccggcgggca
    19021 atccaaatcc tcatgatctc ccggtgataa caggactgga gcttcttcat accaggaact
    19081 cttcaccccc ggcaggcagt ttcaattgtt ctgacagtct attcaactcg atctacaagc
    19141 tcatcagctg ggccataaag agcaatctgg ccagcgttgc taccgactgc ccgcacagag
    19201 aaaagcttgg ctggctggag cagacccatc tgatgggcaa ctccatcagg tacaatttcg
    19261 atatcaggaa cctttacagc aagataatcg acgacatggc cggggcccag cttgaaaacg
    19321 gcctcgtgcc cgatattgct cccgagtatg taccttttta tgcaggtttt cgcgattccc
    19381 cggaatgggg aagtgcgtgc acaatacttc cctggcagat gtatgagtgg tacggggatc
    19441 tgggtgcagt aaaaaaagcc tacccaatga tgaagaaata cgtcggttat cttgaaagca
    19501 gggctgacag gtatttactt agccacggac tgggcgactg gtatgatctt ggtcctaaat
    19561 ttcccgggga ggcacagctt acgcccaaag cactgactgc aacatccatc tggtatcatg
    19621 acgtggatct ccttgcaaga atggccggcc tgaccggaaa cactgacgat caggctcatt
    19681 acaggaagct ggccggaaat atccgcgaag cgttcaacaa ggaattcttc aatcctgaaa
    19741 caaaggttta ttcaacgggc agccagactg cgtattcgat gccgctttat ttcgggatgg
    19801 ttgatcctgc ctgtatcccg gaagtgacgg aaaatcttgt aagctcggtc atcgctggca
    19861 ataaagccct caccgccggt gatatcggtt acaggtatct cctccgggtc ctggagcagg
    19921 gcggacactc acagcttatt tatgaaatga attcgaggac cgatgtaccg ggttacggtt
    19981 accagctttc aaggggtgcc acatcgctca ccgaatcatg ggctgccctt cgcgatgttt
    20041 cgaacaatca catgatgctg ggtcatctca tggaatggtt ttacagcggc ctgggaggga
    20101 tacggcagat accgggttca agagcctgtg atagtatttt catctgtcct gaacccgtga
    20161 aaggcattga ccgggcggag acaacatatc tttcgatccg gggtgaaatc cggacttcat
    20221 ggaagatctc ggaaaacact ttcacactga aagtgaagat cccgatggga tgcagggcaa
    20281 ccatatccct gcccggaacc gatcctggca ggataaccga agatgatact tccattttta
    20341 gttccagcca tgtaaaagtc aggggaaccg gaaatggcag ggtactgtgc gaggtgcctt
    20401 caggggaata taatttcaaa acaacctttt gatcaattaa cggggaaaac tgtattttta
    20461 tttctttcct gtttaacaaa actgttcata caatgagtta caagggtatc atttcaaaat
    20521 ttgccatatt cctgtccctg ctgcttctgt gctattgcag gaacgagact actccccggg
    20581 cggcgggcag cctgcagcgc agcacccctg aagccgaagg ggtcacatcg gaaagtatca
    20641 taggcttcat taaggcagtg gaagcctcgc cccatgagtt acacagtttc atgttcctca
    20701 ggcacggcaa agtcattgcc gaagggtggt gggatcctta cgaacccggg ctgaaacata
    20761 ccctttattc ggccagcaag agctttactt ccactgcagt ggggtttgcc gtaacggaaa
    20821 acctggtaaa ggtcagcgac aaagtcataa cgttcttccc ggaattcctg cccgacaaag
    20881 tgtcaccctt ccttgctgat atggaggtca gggatcttct tacaatgtcg gcgggtcaga
    20941 ctcccgatcc gacccgcatt ctgaccatga atgaaaccaa ctgggtaaag ggtttcctgg
    21001 cacttccggt caaagatgat ccgggaacca aatttcttta taattcgatg gccacctaca
    21061 tgctctcggc cattgttcag aaagttaccg gggagaaatt gatagattac ctgacaccca
    21121 ggctttttga gccgcttgga ataagcggaa tggactggga aacggatcct gaaggcatca
    21181 ataccggggg ctggggtctg aggctgaaaa cagaagatat ggccaggttc gggcagttat
    21241 atctgaacag ggggatgtgg aacggcaggc agatccttcc tgccgcatgg atcaatgagg
    21301 caacgggctt caagatagat cagatgccgg gtgcagtgca ggcagtaagg gattcgagcg
    21361 actggctcca gggatactgc taccagttct ggagatgcag gcacaatgcc tacaggggcg
    21421 acggggctta cggccagtac attattgtca tgcccgaacg cgatgctgtt attgcaataa
    21481 catcagaaac tcccgacatg cagaaggaat taaatctcgt atggaaattc cttttacccg
    21541 gcatgaagga gcagaagctc ccggagaatc ctgcatcata cacagaactg aaaggcatgc
    21601 tgtcagatct tgaactttca ccgggaacgg aaaaacctga tccacaggtc gcggccatgg
    21661 taagcggcag gaaatattca tttgcacaca atgaccggaa cctcggggca atcagtttta
    21721 aatttgccgg tgatgtatgc aatgtcatcc ttaccgccgg cgaaacagat tattttttca
    21781 gtttcggcaa cgggaaatgg ataaaagggg aaaccgaaaa aaagggcccg aatctccttg
    21841 aaaatgcccg tggtcatttc gcgggactgg gtcctgcgaa gacagcgtgt tcgtacaact
    21901 ggcaggatga aaacacaatt acgctgatac tgcggtacat tgaaagccca cattccgaga
    21961 cctatacatg ccggtttgaa ggagacagca ttgaggtcgc actgaaaatt agtttcgcat
    22021 ttgaatcaga gccggttatc ctgaggggaa atgcagtaaa taactaattt tacaggactc
    22081 cggctttgtc accagccggt gaaacaagaa agatataata aaggaagaaa aagggtttta
    22141 ctgatcagta tatttgaatt atgggaagat atgtaaataa tcatctgata cgggatgaat
    22201 cggtcgttta cgaaacaagg cttcactggg tgattttcct taccctgaag tcgcttttca
    22261 ccctcggcat actgcctttg ctgcagagga agctatcgga atttgtaata acgaaccgca
    22321 gggtgatcat caagacaggt ttcattgccc ggagcacgtt tgagatgaat ctctcaaaaa
    22381 ttgagagcgt caacgttgat cagaacatac ccgggaggat attcaattac ggatctatta
    22441 ccataatagg caccggcgga acgaaagaga catttaacaa tataacccgc ccgctcaatt
    22501 tcagaaaggc attccaggaa ctatgctgat tttcaaagcc gattcttccc tgaaatacat
    22561 caaatgggcc ggttaatgca gatcataaag cattttcagg gcctttttca caaccatgtc
    22621 ggaagatcct attgcaaatc ttgtcgttgt gacttcatag ccgccctctt tgtagaattc
    22681 ggctggtacc aggtaaccac ttgagccgtt gcagtgggtg ataatgaacg tatgtctgaa
    22741 aggtgatcct tttttgatct ccaggcctat ttcagtaatc atttcaacat tgaagccgac
    22801 aaatgccaca tcgtcaccga tactggcgac agtgatattt acaggtatgg tcggagactg
    22861 tggctgagcg gcgcctgcct gatcagctgc ggtttctctc cgggggcact ccagtgtttc
    22921 aaatgaggtc ctgatctcac ctccgggttt caattcctta attcccctgt aaacatggac
    22981 aacttcctcg cccagcaatg ttcccagcaa aacaggttca ggtatccatc cgggttcttc
    23041 gttaaaaccg ggaagaaccc tgaaccacgg atcaatgttc ccggaagccc ctgcaaatgc
    23101 cggcgtaata actttgccgc caagaatttt ctcaacaaac tgtgaggaaa gacccaggat
    23161 atccccgcta attttcatac tcgatcctcc aagggaggtg caatgggttg cataatcgta
    23221 gagagcaccg agactgttcc cgtccccggc agcaattttc aggacgagaa cctctttgtc
    23281 ggtcacgcca tagggatttc tgccaagggt tattgatccg tccggcagca tttcacgtct
    23341 gttcaccccg actggtgatg ctccggtccc cgttcctgtc agaacaggct gcatgtttct
    23401 gaaagcttcc cctatcagat caagcagttt tttctgaagt cccttcgtgt actcaatatt
    23461 gttaggatca cctctttcag gattgagggt cagtatcggg gcgctgtgat tgtggatagc
    23521 tgagaggaat atttctgatt ccctgagatt gaatctatcc ataatgcttt tctgaaacac
    23581 cgtaaagaca tcccttccaa atgatccgag atcggttgaa accagcagaa gtttcctgcc
    23641 attgttctcg aaagcaacag cccgggccga caacgggtca tgcacgcctt ccgaatatgt
    23701 ttttctggca gcatatccat acagtttaac cggtatctcc ggtgtgatat cggtttttgc
    23761 gaatcctgca cgcagtcctg tcggcgcaga gtcggatccg cctgccgctt caacacagaa
    23821 aaagccgagc agcggaaaaa gaagggcaat tactgaattt ttcatcggtt tataatttat
    23881 ttgtaaaggt tcggaaactg tctgacccgt cggaccgggg agtcttcatt ctgaaaatga
    23941 tacttacatg tatttgtgca ttgtaaacat ccggatttca taacaatgat ccgtttaagt
    24001 tacaaaccat ggcaaagccg ttctgcaacg gaggcagaat acccgaaaat ttatatcaat
    24061 aatgaccggc ctccaggttc aacctgtttg gtcaataatt gaaacctttc gtttatcaaa
    24121 tataaatcaa attgcataaa aaaagagatt tttgttaata ttttattatt caactcatta
    24181 aagagtgttt ttgtttaaca taagatagca cggaaggtat tggcataagt tttgtcatga
    24241 ggtaagtgta aacaggttct tcaaataaac aaagtcatga aacgattctt aatcacctcg
    24301 atctttgcag cgatagttgc agccgggaca gcgatgatgc cccaggagta cccgaaagaa
    24361 tatctgggtc tgccgggtga taatcttaat ctttatgccg taatgaaatt gttccaggaa
    24421 tctgaaaccc tcgagggttt tgaaaggagc ctgaacgatg aaaactcaag gatcaacaat
    24481 cttgacctta acaatgacaa ctacattgat tatatcaagg ttcttgacta cgttgacggg
    24541 aatgtgcata cgatagtgct tcaggccgtt ctgggccgga atgagagcca ggacgttgca
    24601 gtgttcaccg tggaacgatt ccgtgacggg tcggtacaga tacagctggt aggcgatgaa
    24661 gcattgtatg ggagaaacta cattgtggaa ccctattatg cagagacgcc caacccgggt
    24721 tataccggga ccagggttcg caacaacaca acggttgtga gaaccactta tgtagaggtt
    24781 gcagcctggc cggtaataag gttcatctac cttcccggat atacggtatg gcggtcatcc
    24841 tggtactggg ggtattaccc ggtttactgg cgtccctggc gaccctacta ctggcactac
    24901 tactacggtt atcactacca ctggttccct gaatactaca ggtactacca caggtatgac
    24961 cgtctgcggt ggaccggatg ggacaggtat tactaccgcg acagaagggt ttactccaca
    25021 aatgtcacga ttaacataaa cacaggaaag tacagggata cctactcacg tcctgatcag
    25081 agaaaggcag gagaggcaat gtatgccaag gcgcatcctg aaagggctgc cacaatgtcg
    25141 gcaactggca gcaggagatc tgcagcagat gcatcgacat cgaggcaggg aagcgggact
    25201 tctgtgaacc agggcagaag gtcttccggc actgtcagca ccggcaggag cacttctcct
    25261 tcgcagggac aggcaggcac cagcaggaga tcatctgaga cagtcaacca gaacaggggt
    25321 acagctcccg tgcagggaca ggccggtacc agcagaaggt caactgaaac cgttaaccag
    25381 aacaggagtt cagctcccgt gcaggggcag gcaggtggaa cacgccgatc cggcagccct
    25441 gcaatgaaca gcggcacccg ttcaaccccc caggctggac aggctgcgac aggcaacaga
    25501 agatcaccgg aagctgcagc aagaccggct cctgaatcaa ggagccaggc ttctgctccc
    25561 agggcggcat cttcagccag gcgcgggtca accggagcgc cggca
//
LOCUS       DAOOYG010000027        25550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1726_length_25550_cov_8.676878, whole genome shotgun sequence.
ACCESSION   DAOOYG010000027 DAOOYG010000000
VERSION     DAOOYG010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25550
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1726_length_25550_cov_8.676878"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PK288_04130"
     CDS             <1..306
                     /locus_tag="PK288_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HPM18140.1"
                     /translation="KKRISGYKFLRQHPLFYKIDGERIEFFIADFYCAGLKLVIEVDG
                     PVHKKRRIYDSDRDSKLNNKGIMVVRILNEELADINHAISKLTQVISQRETEISDLQ"
     gene            1078..1148
                     /locus_tag="PK288_04135"
     tRNA            1078..1148
                     /locus_tag="PK288_04135"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1110..1112,aa:Cys,seq:gca)
     gene            complement(1212..2651)
                     /locus_tag="PK288_04140"
     CDS             complement(1212..2651)
                     /locus_tag="PK288_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HPM18141.1"
                     /translation="MNNWFELVIDLIIILIFLGGFTRFRTPKGARQGNFSAAFALALA
                     VVVIITSYRIMQPHIVILALLIGSALGWLVAMRVNMIQIPAMVAFQHGAGGIAAFLVS
                     FIELTRNTLPGFGVEKISGFLGLAIGALTFSGSMIASGKLANIIKKPPLVLPMHSHIT
                     IGLLILIVLTGYFTGYLQGRPLTILLAVMVAESILLGLVFSIRIGGADMPVLISFLNA
                     TAGLAAAFCGVIIHNRLLIACGATVAASGSILTHVMCKAMNRNLLKVFIGFRVKDSAD
                     PIPAFFGEEIVADNLAGIGDESPAEPPAEVDPLTEAIRVCREAKNVIVVPGYGMAFAQ
                     AQFDVVRFTTLLEQSGKEVRFAIHPVAGRMPGHMDVLLAEAELSYDKMIEMDDINQDF
                     EKTDLAIIVGACDVVNPAAMTTKNTPISGMPILLAHNAKRVLVCNLDDKPGYSGIDNP
                     LYKNKKVIMLLGDAKETLTKLIDRYISES"
     gene            complement(2676..2960)
                     /locus_tag="PK288_04145"
     CDS             complement(2676..2960)
                     /locus_tag="PK288_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPM18142.1"
                     /translation="MSELLIMLSVFILSIIIGYLLISKVPPLLHTPLMSMTNAISAVT
                     ILGALLVFAVVPDIPDTVFGALAIALAAFNVVGGFTITERMLKLFKNKKE"
     gene            complement(2977..4140)
                     /locus_tag="PK288_04150"
     CDS             complement(2977..4140)
                     /locus_tag="PK288_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPM18143.1"
                     /translation="MIIGIPKEIMAEEKRVAATPDTTEKLIKLGFKVIVESSAGAGIF
                     YSDDDYRSAGAEIIDDVAALYGKADIILKVKEPNYNVKAGKHEVEMIKNGSILITFLH
                     PATPSNHAMIRALNDKGITSFTMDGIPRTPNAQKLDALTSMSTVTGYKSVLLAANQLL
                     KFVPMINTAVGSIKPSKFLIVGAGVVGLQAIATVKRLGGIITAVDIRLKAREEAISLG
                     AKIGGFDVPSELATAEGGYAKSLPADWIEREREILKPLVADADAVILSALVPGEVAPV
                     LITEDMIASMKPGSVIVDVSIDQGGNCSVTEAGKQIVRHNVIIDGLQNIPGRVAVHST
                     WLYATNMYNYVENLFKNGDFRPDFDDEIVSNSLVTHNGKIVHAGTRKAMGETN"
     gene            complement(4343..5122)
                     /locus_tag="PK288_04155"
     CDS             complement(4343..5122)
                     /locus_tag="PK288_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HPM18144.1"
                     /translation="MTNKDFRTIVVLIMAALTIYSCNNHSGKTRQAHTSDGRSTQVSP
                     DWHGTYSGFLPCADCDRIETELILNDDFTYVLVNSYLKGEESFTDTLLGKFTLKGSRI
                     KLQGIPKNERSPWFKVEEGQVRYLDMKGRIIKGELENHYIMRKNGNPLVEDRKWQIVE
                     IYGKPVEGTADNYYMIFNSREGRISAKVNCNILNYSYKIKNEFQVRFGQGMSTLMACP
                     DNLEDEFKEILELADNLSTDGKYLSLNKARMAPLARLILAE"
     gene            5372..6142
                     /locus_tag="PK288_04160"
     CDS             5372..6142
                     /locus_tag="PK288_04160"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HPM18145.1"
                     /translation="MQNISSGSGIRSILNFRDAGGLKITGGGVMRKGLIYRSANPDRV
                     NGNDISRLKELGIKTIIDLRGPGESAKRRNLTPDIKLINYPLDFAGITRLKLKPLITR
                     RYNPEEINRVISDLYVELVDAARPVLSKMADLILEPGNVPLLIHCQAGKDRTGILSAL
                     LQMIAGADRQEIIGNYMASNDSMVPHYERKLRIRKFLTLGLFPSDAVLHAIKQKHEDI
                     LTVLSRVENHYGGIEGYLSGSGFDIAKLQNLRKILVDQ"
     gene            6177..6533
                     /locus_tag="PK288_04165"
     CDS             6177..6533
                     /locus_tag="PK288_04165"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPM18146.1"
                     /translation="MKRKKSFRNAFKGLGTVILSQVNFRIHLTVLIVVVVAGLALRID
                     PTGWIAIITVAGLVLAAECFNTAIEFLGDAYTEEENPFIRKAKDIAAAGVLLMSVTAA
                     VVGLIVFIPALLNLLK"
     gene            6541..7662
                     /locus_tag="PK288_04170"
     CDS             6541..7662
                     /locus_tag="PK288_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18147.1"
                     /translation="MEDILIKEVVTRKDLRDFIYLPGKIHRNEPGWLPPLYMAEWEFF
                     SKKKNKSFEYSDTLLLLAYRGGRLAGRIMGIINRRYNAISNEQHGRFCFMECYNDREV
                     FRALLKKIEDWARQKGMKKIVGPLGFSDKDPQGFQIEGFEYEQFITSPNNSPYMVSLI
                     EGEGYEKKVDLVDYLGDIPEQFPAVYGRLLGRNNSTGEFRIIEFSSRKQLKPFIQPVL
                     EIMNETFDEIYAFVPLDDSEKSELASRYLPILDPRFVKVAERDGQLIGFALAIPDVSP
                     AIKACRGKLFPFGFIKILRESRRTGKLLMLLGGIRREHRGKGVDVLMGVKILQEAIAS
                     NMKIIESHLVLEENFRMRKEYERIGCKVIKKFRIYQKDL"
     gene            7851..9272
                     /locus_tag="PK288_04175"
     CDS             7851..9272
                     /locus_tag="PK288_04175"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPM18148.1"
                     /translation="MKQITRRKFMEKAMITGAGVSVMPVMASSGDNNLKQVGNTPPVD
                     TELPGIPGNIIIQPPLADGDALQHDFSTMRPLRIKAPVIVTVGQDEGDLRGKDNIVLQ
                     AAVDYVAGLGGGTVHILPGTYIMRNSLFPKAGVTIRGSGEKTILWKAPSVSSKLIREA
                     DWYEYCVQVENPEGFTPGGGLALSADKKNDQEVSLFTITAIKDNILFLDRRTEKNFWP
                     VDEARATTRFSIIHALNVNDVKVEDIILDGNLSANERVNDNYAAAVFIQYCDNWSFRN
                     VTARNYNGDGFSFQVCDDVRFENCHSLNNFDLGYHPGSGSQRPVFKNCTAKGNSQGFF
                     WCWGVCYGIAENCIASDNRKYGVNFGHRDTDNILRNCLVENNQEIGILFRKEENEFRT
                     GDRNIIENCTIRNNGTAGPGTGIDIQWKTNDITIRDCYFENTRNGPQKTAIKISADAQ
                     RITVENNKFSGDAVKIEDLRKKS"
     gene            complement(9282..10178)
                     /locus_tag="PK288_04180"
     CDS             complement(9282..10178)
                     /locus_tag="PK288_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18149.1"
                     /translation="MIKFFRAASMLAFLIFPGFAPVKNAGNYTLGYNLSKPDAVLILP
                     AILHEISGITHLESDLFACIQDENGIVFFYDLKKNKIERQLHFNINGDYEGIAIAGSK
                     IYVLRSDGALFEIAGHESGNLNFDVFYTNIPSRNNEGLCFDPDGNRLLIAPKGKTLKG
                     PADKDKREIYAFDLKTRTLSQDPVFIFDLQAIRNFAKANKLNLPLKEKKKGKQPEPRI
                     DFMTSAIGINPVSKKLFILSSSDYLLFISDMDGNIEHIETLNPAVFNNAEGIAFYSNG
                     DMVITNEGGSGNPTLLLFKYQE"
     gene            10450..11046
                     /locus_tag="PK288_04185"
     CDS             10450..11046
                     /locus_tag="PK288_04185"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPM18150.1"
                     /translation="MSDNFFNLNHSDFFGILMRFAINTIFLIILIRVIYFRYSRKEKF
                     LFTFFLMGITVFFITSMLKSVFIEFGMAIGLFAIFAILRFRTRNFSLKDMSYIFTTIG
                     ISVINSLKLTGFPVLGVIIFNIIIMVSAVILEEFTVKHNIVSHTIIYEDLDLLKSAKK
                     QKIIRELSALTGKDITCVRIRKINYKDKVAFLDISYRD"
     gene            11156..12757
                     /locus_tag="PK288_04190"
     CDS             11156..12757
                     /locus_tag="PK288_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPM18151.1"
                     /translation="MQIDRRRFIRSSVLAGAGLVLSPGKGLCDENTSDYFGVHPFILM
                     NPDAVFIMKTNVDVKTNSSVIKKTGLDFGRSVFCNTGDKENGFPKTGKIVIKPNLTCR
                     ARNSSAYTIERSMGIVTDAFFVEGVIESLKELGLSSNQLFIREVNCPDDFSDGGYISM
                     AERTGIDLQGINTPVQNLAPEQVSWVNVPGGVFFRRIPYLWPVNAPGTFLINIAKFKT
                     HGMGMTLCAKNLQGTIAANYQAHCSAYGRSISGVSASDLNPAAASDILANYNRHRVAG
                     IPRWDKPGSDGGLWQEIWATRCLDNNSVTFAGLHIIEGIYGRDGDFMTGPAQNGLATD
                     YMTNIIIFGRNQFHVDNIGHYLGGHEPGNFGLFHLALERGMAKTINPGEIPLYEWDIH
                     TGARPVSLESQQRFRLKTYYLQRNYNGQDEPYWHLVNEPYEYDSAGVNDPGFSRSGFH
                     LQQNFPNPVNTVTSIPFRLPSGGNARLEILNTSGKLIEVIVNRNLEGGTHTVLWDSRT
                     VPSGSYICRIHFNSMNSTIWMIVIH"
     gene            12895..14031
                     /locus_tag="PK288_04195"
     CDS             12895..14031
                     /locus_tag="PK288_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPM18152.1"
                     /translation="MRFNSIPFILLLLAGILFCNSCNRTPYYGPDIYVPDTTFKVVGY
                     LSTGNFDKIDALELDKLTYLNIAFANPDADGNLVAELNADIKPVVQKGHDLNLKVFIS
                     LAGGGRPDTVIWNNLLKPENRSAFIKKLVAYVEENNLDGVDVDIEGNLLPYLDNNYNP
                     FVLELRKALHAKGKGITAALGAVGFHEAVGQEALEAYDFINVMVYDKTGIWRPHDIGP
                     HSPYSYATDAIKFWTIDRKIPPERITLGVPFYGFDFTPPARYISYAEIIRENKGYAYH
                     DSVGTRYYYNGIPTMVKKTELAKKTLGGVMIWEIAYDTTGDLSLMKALYQTLEAGNCQ
                     VRTFFRDEDGDGFGDMSRPIQACEAPEGYVANMDDRDDKNPRVH"
     gene            14215..15690
                     /gene="araA"
                     /locus_tag="PK288_04200"
     CDS             14215..15690
                     /gene="araA"
                     /locus_tag="PK288_04200"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HPM18153.1"
                     /translation="MKIFNYELWFVTGSQSLYGEETLKQVAEDSRTIAAALDNNHLMP
                     ARIVFKPVLTTSDAITKLCLEANSDQLCAGIITWMHTFSPAKMWIAGLSRLHKPMLHF
                     HTQFNRDIPWKEIDMNFMNLNQSAHGDREFGYICSRLRLNRKVVTGHWQDNSVIGEIA
                     AWVRSAVAFTEAGSLKVARFGDNMRDVAVTEGNKVSAQLELGYEVHGFGVGDLVSEVN
                     AVSATAVKSLVEQYGDEYMVQPEVLNSPPVREAARIELGMRNFLEKGSFKAFTTTFED
                     LHGLAQLPGLAVQRLMAAGYGFGAEGDWKTAALVRCMKVMAAGLEGGTSFMEDYTYHL
                     DPGNPAVLGAHMLEVCPSLADGKPSLQVHPLGIGGKNDPARLVFKTRTGEALNASLID
                     LGDRFRLVVNPVEVIDCPDMPKLPVASVLWKPRPNLKTAATAWIYAGGAHHTGFSQAL
                     NTGHLVNFAEIAGIECIVIDDKCRVDEFKKELRWNDLFYHI"
     gene            15695..16384
                     /gene="araD"
                     /locus_tag="PK288_04205"
     CDS             15695..16384
                     /gene="araD"
                     /locus_tag="PK288_04205"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HPM18154.1"
                     /translation="MLQNLKEEVYRANLSLVEHKLVKLTFGNASGIDRKTGFMVIKPS
                     GISMRDSGPDDMVVTDLSCNVVEGDLLPSTDAPTHAALYKAFGTIGGIVHTHSASATA
                     WAQACRPIPCLGTTHADYFHGNVPVTRSLTREEIAGNYEYNTALVIVECFREMDPLRI
                     PAVLVASHGPFTWGGDVTQAVLHAISLEEIARTAALTLSIAPVGPIDQTLLDKHFFRK
                     HGQGAYYGQKK"
     gene            16543..17115
                     /locus_tag="PK288_04210"
     CDS             16543..17115
                     /locus_tag="PK288_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPM18155.1"
                     /translation="MTKLSVLWKIRLVIVMIIIAQLTVSCSKSGQKDDETPPVLSVTP
                     VDITTVTHIIPFGADLSPQQKNPAFEYYVSNSSVQVRSVCAGYVEDIRLNDNFPDYEV
                     WIKTSSNSVYKLIYDHIVDLNVVKGNKVDAGTVLGKVGKGNRTELQINKSDRNMGELS
                     YCPFDFATDDFISSHKQFTDTWCLTGTVRP"
     gene            complement(17355..18599)
                     /locus_tag="PK288_04215"
     CDS             complement(17355..18599)
                     /locus_tag="PK288_04215"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPM18156.1"
                     /translation="MKRKLFCAIASIMFAIPIVINAQNKDKAVFRTLPPGFYQNSILR
                     DDREARERAEPARPMKMFAVDLSSSSLPNKFSMYRNQQWHNPPVSQGSTSTCWCFCTT
                     SFLESEIYRIHKMKVKLSEMYTVYWEYVEKARRFIRERGNSLFDEGSEANAVTRMWSK
                     YGIVPYEDYTGLLNGRKFHNTDRMREEMQSYLQSVKESSSWDEDAALATIKSIMNHYM
                     GEPPAEITVNGEKMTPVQYLRDILKINPGDYVDILSYTQEPFYKKVEYQVPDNWWHNS
                     DYHNVPLDVFMTALKNAVRKGYTVSIGGDVSEPGFDRVTQCAVIPDFDIPSAYINDDA
                     RQFRFSNRTTTDDHGMHLVGYLEKDGKDWYLVKDSGSGSRNNDPDAAEFGYYFFSEDY
                     VKLKMMDFMVHKDAVKELLQKF"
     gene            complement(18837..19808)
                     /locus_tag="PK288_04220"
     CDS             complement(18837..19808)
                     /locus_tag="PK288_04220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPM18157.1"
                     /translation="MKYRLFGKTGWRISEIGYGMWGMAGWTGSDDEESFRSLQLAADL
                     GCNFFDTAWGYGAGKSEHLLGELIRANRDKKIYTATKMPPKNFQWPSTREFSLDDCFP
                     PDHIEEYVSKSLKNAGLDSFDLMQFHTWEESWLDDDRGIKKMSDLKSQGLFHAIGISL
                     NRWEPWNGIRAVESGLIDSVQVIYNIFDQNPKDELFPACRKRNVAVIARVPFDEGTLT
                     GTLTKTTTWPEGDWRNTYFVPENLIPSVERADALKPLLPEGMSMPEMALRFILGEPTV
                     TTIIPGMRKPGHVRSNLSASEKGPLPAKLMNELEKHRWNRVPTKWSQ"
     gene            complement(20000..21112)
                     /locus_tag="PK288_04225"
     CDS             complement(20000..21112)
                     /locus_tag="PK288_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18158.1"
                     /translation="MRSLLSIFALITILICSFSPVNTLTKTEPPGKTGHFQARGIYGS
                     PDAFWNRGIELNRLNVNSVFMNWKAINDRIMERAGKEGLKVYAEFPVLNGAGYVDKHP
                     EAWAIDSDGKRVEPASWFMGVCPTDLGFRQFRMNELRQLVGRFELDGIWMDYLHWHAQ
                     FEEPEPILPETCFCDNCISSFETATGIRVPGMSTPEKAEWILKMHEAGWREWRCSVIA
                     DWVRETREIVKEERPGALLGIFHCPWEDGEFNGARVRILGLDYDLLKDLTDVFSPMVY
                     HGRMGRKPEWVRENIEWFCRRLDISPGAFPKVWPIVQAYDDPRAITQEEFRKVLNYGS
                     AVPVTGIMMFTANAVAENPAKTETMKQVYSGLFSAN"
     gene            21494..21763
                     /locus_tag="PK288_04230"
     CDS             21494..21763
                     /locus_tag="PK288_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPM18159.1"
                     /translation="MEKVLYKIYVRGRVQGVGFRYSAVRAANHHGITGFVKNMPDGSV
                     YMEAEGTEKELLDFIEWCRNGPGHVGSLTIDPYPPRNYVDFRIVF"
     gene            21839..23821
                     /locus_tag="PK288_04235"
     CDS             21839..23821
                     /locus_tag="PK288_04235"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HPM18160.1"
                     /translation="MKKYLILVMSVFINLPVSSQKYEIRSPDGSLTAGIEISNGIDLS
                     IFKGSLPVLKLENIGLVASDSENPFSEFKVQKTVRRRVNEVVRPEIREKASELINSYN
                     ELEIRFRSKFAVTFRMFDEGLAYRFSTSLKNELTIYRENLELCFEDGDSVRFQSSGTF
                     NSAYETPYEHEKPAGIGDGKLCNLPFLVEKKDGTFIMVTEADLYNYPGLWLKGTGQPR
                     LSAVNPPCPKTLTATGSPYNLGQVEEAYGYIARVAGTRNFPWRIFAVAGDEAELVSNN
                     MTYLLASPCAISDVSWIKPGVVMFDWWAKHNIYGVGFRSGINTETARYFIDFCAKHGF
                     RYFLFDDGWSPGEDLLKTVPGLDIREVTAYGREKGVDIMLWVIWHTLGMQWDEAFNQF
                     EKWGIKGIKVDFMNRDDQPMIEFYEAVAAEAAKRKMVVDFHGACKPDGLRRKYPNVLT
                     REALIEFEFNGWTSYDDPVHHNLLPYIRMFTGPMDYIPATMRNSTRENFRPVGDYPMG
                     QGTRAHAMALFVILNSPMTMLPDSPSDYYREQECTGFLAGIPVVWDETRLLEGKIGKY
                     TVLARRSGREWYLGAITDWDKRTISLKTDFLEPGRYWLEAVEDGINADKRAEDYIKVR
                     KEFQAGDSLVVNLAPGGGWVARIMPVNQNTASGRGN"
     gene            complement(23877..>25550)
                     /locus_tag="PK288_04240"
     CDS             complement(23877..>25550)
                     /locus_tag="PK288_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPM18161.1"
                     /translation="VGPQNLYNLNLTFETKGSVSDIKKVRFGIREISSWMNDFDGRHT
                     RVFQINGKNIVIRGGGYVEDMLLRPSDERIDADIRYAKHMGLNTLRLEAPRGSDYLFE
                     KCDEEGILLMVGWCCCSSWEMWDRWTPEMEIVAQKSWEDLILRLRNHPSVFDWLYGSD
                     MHPTEKIEKMYLNVLNELDQTRPFQSSATQDPSTVTGNTGVWMGPYPDVYSYMPPSYW
                     YGKLEFNTEAGPSGEQIPPVESMRKMMPEQDLWPISDSWNIRLNKAFFPAARKALFSR
                     YGKPENLNEYSMKSQVLQYEASRAMFEAYAGNKYKSSGIIYWMYNSAWPTMYWQLYDY
                     FLAPNGAFYGIKKACENLHIQYSYDDHSVRVVNGLYRDFSGLKASAKLYDMKMNEIHS
                     EEVPVAVKADESVKVMFLEKPRPGSGITFLKLILNDDSGRELSSNFYWLSSKGDENAD
                     FTALNRLPEVELSCSVSPVLKENGRYRAVIEIENPSDFLAFSVNPKIIRRESGDLVLP
                     VFWEDNYFALLPKEKRSVKVEFSDQDLGGEIPVLAIDGWNIRKAEQELK"
BASE COUNT         6853 a         5665 c         5985 g         7047 t
ORIGIN      
        1 aagaagagaa ttagcgggta taaatttcta agacaacatc ctttgtttta caaaattgac
       61 ggtgagcgga ttgagttctt tatagctgat ttttactgtg cggggctaaa actagttatc
      121 gaggtcgatg gaccggtgca taaaaagcgc aggatttatg actcggatag agattccaaa
      181 ctaaacaaca aggggattat ggtagtcaga atactgaatg aggaattagc agatataaat
      241 catgcaattt caaaactgac acaggttatc agccaacgtg aaacggaaat ctccgacttg
      301 cagtaatctc ccccccttcc cttattgttt aagggaaggg gtcgggggaa gggttaagct
      361 acctggaata tctctcaacc catctccctg tctcttccct taagcattaa gggaagaggt
      421 tgttgcaata gttttagata atacagtacg caaattgaat aatcttaact tttttatttt
      481 cagtttatct ataatgttgg aataaactcg tcatgccacg ttgcagtaac cttccctttc
      541 ccttgttgtt taagggaagg ggtcggggga agggttaaga aacctggaat atctcttaac
      601 ccatctccct gtctcttccc ttaagcatta agggaagagg ttgttgcaat agttttagat
      661 aatacagtac gcaaattgac taatcttaac ttttttattt tctgcttacc tttaatgttg
      721 gaataaactc gtcatgccac gttgaagtaa tctccccctt cccttattgt ttaagggaag
      781 gggctggggg aagggttaag aaacgatata gagaggctcg gggtgagggt taagacagag
      841 ggataaagca tgaagcatac aagagagatg gttttgataa aggatttttt attacttttg
      901 caaacaatat tattggtcag ttgtccgaat ggccaggaat tcccgagttc tcgggacaaa
      961 accggctatg gcggttcgaa ccgactgaac gaagtgaagg agctcgcgag ctgtcaggcg
     1021 agcaatccgc cactgacctt acaggttttt tgaaaatagg attattacag gtttattggt
     1081 cagttgtccg agtggctagg aatcggtctg caaaaccggc tacggcggtt cgaatccgcc
     1141 actgacctca acaaaaatcc ccctgtgcga tgcgaaaggg ggattttttg tttttagtcc
     1201 gtttcatgct gtcagctttc gctgatatac ctgtcgataa gttttgtcag ggtttccttt
     1261 gcgtcaccaa gcagcatgat tacttttttg tttttataca gcggattgtc gatccctgaa
     1321 tagccgggct tgtcgtccag attgcagaca agtacccgct ttgcattgtg tgccagcagg
     1381 atgggcatgc ctgagatcgg ggtgttttta gttgtcatgg ctgcagggtt aaccacgtcg
     1441 caggcgccta caattatagc caggtcggtc ttttcgaaat cctggttaat gtcatccatt
     1501 tcaatcatct tgtcatagga aagctcagcc tcagcaagga ggacatccat atgccccggc
     1561 atcctgccgg ccacggggtg gatggcaaac ctgacttcct ttcctgactg ttccagcagg
     1621 gtggtgaacc tgacgacatc gaactgggcc tgggcgaatg ccattccgta accaggtact
     1681 acaattacgt tttttgcttc cctgcatact cttatggctt cagtcagagg atcgacttcg
     1741 gccgggggtt cagcaggcga ttcatctcca atgccggcca gattatccgc tactatttcc
     1801 tcaccaaaga atgccgggat cggatctgcg gaatccttta ccctgaatcc tatgaaaacc
     1861 ttgagcaggt ttctgttcat tgccttgcac atcacatggg tcaggatcga gcctgaggca
     1921 gcaactgtag ctccgcaggc tattagcagc ctgttatgga ttatcacccc gcagaaggca
     1981 gctgccagac cggctgttgc attcaggaac gagatcagaa caggcatgtc ggcgccgccg
     2041 atacggattg aaaagaccag ccccaacaga attgattcgg caaccattac tgcaagcaaa
     2101 atggtgaggg gacgaccctg caggtagcct gtgaaatagc ctgtcagaac tatcagaatc
     2161 agcaatccga tggttatgtg gctatgcatt ggcagcacaa gcggtggttt tttaatgatg
     2221 ttggcaagct tcccgcttgc gatcatgctt ccgctgaagg ttagggcacc aatcgcaagc
     2281 cccagaaatc cggaaatttt ctctacacca aaacccggaa gggtgtttcg ggtaagttca
     2341 ataaaggaaa ccagaaatgc cgcgattcca cctgccccgt gctgaaacgc caccatcgcc
     2401 ggaatctgga tcatattcac cctcatggca accagccagc ccagggccga accaattagc
     2461 agtgcaagta ttactatatg tggctgcata atcctgtagc tggtaattat cacaaccact
     2521 gcaagtgcaa gggcaaaggc agcactgaag tttccctggc gtgccccttt cggagttctg
     2581 aacctagtaa atccgccaag gaatataagt atgattatta ggtcaataac taactcaaac
     2641 cagttattca tcgggctagg aataagttcg gattattatt cttttttgtt tttgaacaat
     2701 ttcagcatcc tttcggtaat ggtgaatcct cccacgacat tgaatgctgc cagagctata
     2761 gccaaagcgc cgaagacagt atccgggata tccggaacaa cggcaaaaac cagtagggct
     2821 cccagtatcg ttaccgctga aatggcatta gtcatcgaca tcagaggtgt gtgaagcaac
     2881 gggggaactt ttgaaataag cagatatcct attattattg acaggataaa aacagaaagc
     2941 attatcagta actcgctcat gactgtttta ttaaattcag ttagtttcac ccattgcctt
     3001 gcgtgtgcct gcatggacta ttttaccgtt atgtgtaacc agtgaattac tcacaatttc
     3061 atcatcaaag tcgggtctga aatcgccgtt tttaaaaaga ttctcaacat agttgtacat
     3121 attggtcgca taaagccagg ttgaatgaac ggcaactctt cccggaatat tctgcaatcc
     3181 gtcgatgatg acattatgcc tgacaatctg tttgccggct tcggtaacgg aacagttgcc
     3241 gccctggtcg attgaaacat caactatcac tgaaccgggt ttcatagatg caatcatatc
     3301 ctccgtaata agtaccggag ctacttctcc cggaacaagg gcgctcagta ttacagcgtc
     3361 tgcatccgca acaagtggct tgagaatttc tctctctctt tcaatccagt cggctggaag
     3421 gcttttagca tatcctcctt cggctgttgc cagttcggaa ggcacatcaa atccgcctat
     3481 ctttgctccc aggcttattg cttcctcccg ggccttcaat ctgatgtcaa cagctgtgat
     3541 aatgccacca agccttttaa cagtggcaat tgcctgcagg cccactactc cggccccgac
     3601 aatgaggaat ttagagggtt tgatcgatcc tactgcagta ttgatcatcg ggacgaactt
     3661 tagaagctga ttggcagcaa gaagaacgga tttatatccg gttactgtgc tcatggatgt
     3721 aagggcatcc agcttctggg cattcggggt gcgtggaatt ccatccatgg tgaacgatgt
     3781 tattcccttg tcattcaaag ccctgatcat tgcatgattg gatggtgtcg ccggatggag
     3841 gaaagtaata aggatacttc cgttctttat catttcaacc tcatgcttac ccgctttaac
     3901 attgtagttg ggctccttga ccttaaggat aatatctgct ttcccgtata aagcggccac
     3961 atcatcaatg atctccgcgc cggctgatct gtaatcatca tcactgtaaa atattccggc
     4021 accggcagat gattcgacaa tgactttaaa acccagttta ataagttttt cagttgtgtc
     4081 aggagttgcg gcaactcttt tttcttctgc cataatttct ttggggattc cgattatcat
     4141 aataaacctc ccttttaatt agttgaagtg ttgttagtta attgtcctga aatggaaaaa
     4201 taatttctcc aatgtaactt aataattcat tattttacat gataaatgtt tctttttggc
     4261 cttcagactt atttgcagaa gacagcctta ttcattatgg agcggagttg ggatcctgtt
     4321 cagccttcct gtttccgggt ggtcattctg ccaggattaa tcttgcaaga ggagccatcc
     4381 ttgctttatt cagggacagg tacttaccgt cagttgacag attgtctgcc agttccagta
     4441 tctccttgaa ttcgtcttca agattatcgg ggcaggccat cagtgttgac ataccctgcc
     4501 cgaaccttac ctgaaattca ttcttgattt tatagctgta gttcaggatg ttacaattta
     4561 ctttggcaga aatccgccct tccctggaat tgaagatcat ataatagtta tctgcagtac
     4621 cttcgacggg cttaccgtaa atctccacaa tctgccactt cctgtcttcc acaagcggat
     4681 ttccgttttt cctcattata taatgatttt caagttcacc ctttattatc ctgcctttca
     4741 tatcgaggta ccttacctgt ccttcctcta ccttgaacca cggggatctt tcatttttgg
     4801 ggatcccttg cagtttgatc ctgcttccct tcaatgtgaa cttacccagc aaagtgtcgg
     4861 taaaactctc ttctcctttc agatagctgt tgaccaggac ataggtgaag tcgtcgttca
     4921 ggatcagttc tgtttcaatc ctgtcacaat cagcacaggg aagaaaaccg gaatatgttc
     4981 catgccagtc gggggaaact tgtgtactac gtccgtcgga tgtatgtgcc tgccgggttt
     5041 tccccgaatg attgttgcac gaataaatag ttaatgcagc catgataagg actacaattg
     5101 tcctgaaatc tttgtttgtc atcgattcta atttttcctg aggcaaatat aattgaacca
     5161 taccttaata ccaattcccg gcatcataat caggatgtaa ccgattccgg aaaaattatt
     5221 gggtgacaga ggtcaaaacc tgataaaaca agtcattaaa gtagtatgtt tacattcttg
     5281 gataacccgg ttaatgaaaa ccgggattgt attcttatta caattgtttg gctgtgattg
     5341 cagtgaaaca attattatta attttaccgg aatgcagaat atatcttcag gttcagggat
     5401 caggtccatc ctgaatttca gggatgcagg aggattgaaa atcacgggag gcggtgtaat
     5461 gagaaaagga ctcatttaca ggtcagccaa tcccgaccgg gttaatggaa atgatatttc
     5521 aaggcttaag gaattaggaa taaaaacaat catagacctg agggggccgg gagaatctgc
     5581 aaaacgaaga aacctaacgc ctgatattaa gctaatcaat tacccgctcg attttgcggg
     5641 tataacacgg ttgaaactca aaccactgat cacaagacgt tataatcctg aagaaataaa
     5701 cagggtaatc agtgatcttt atgttgagct tgttgatgca gcgcgaccgg tattgagcaa
     5761 aatggcagat ctgatcctgg aaccggggaa tgttcccttg cttatacatt gccaggcagg
     5821 caaggacagg accggcatcc tgagcgcact cctccagatg atagcaggtg ccgacaggca
     5881 agaaatcatt gggaattata tggcatcaaa tgactcaatg gttccgcact atgaaagaaa
     5941 gctgagaatc agaaaattcc tgacgctcgg tttattccct tccgatgcag tactgcacgc
     6001 aataaaacag aaacatgaag atatcctgac agtgctctcc agggtcgaaa accattatgg
     6061 cggtattgaa gggtatcttt cgggttctgg attcgatatt gcaaagcttc agaatctcag
     6121 aaagatactt gtagaccaat agaataatgt taccggaaat tatgatttct ttttttgtga
     6181 aaaggaagaa gagcttcagg aatgcattta agggactggg taccgttata ttgagccagg
     6241 tcaatttcag gatacatctc acggtcctga tcgttgttgt tgttgcggga ctggccttaa
     6301 ggattgaccc gaccggatgg attgccataa tcaccgttgc agggctggtt ctggccgccg
     6361 aatgttttaa cacagcaata gaattcctgg gtgatgctta tacagaggaa gaaaatccat
     6421 ttatcagaaa ggccaaggat attgcggcag cgggggtatt actgatgtct gtcactgcag
     6481 cagtggtggg cctgattgtt tttatcccgg cattgttgaa tttattaaaa tagccacagg
     6541 atggaagata tcttaataaa ggaagttgtt acacggaaag accttcgtga tttcatttac
     6601 ctgccgggga agatacacag aaatgagccg ggctggcttc ctccattata catggctgag
     6661 tgggaatttt tcagcaaaaa gaagaacaag tctttcgagt attccgatac cctgctgtta
     6721 ctggcctaca ggggcggaag gcttgccggc aggatcatgg gtattatcaa caggcggtac
     6781 aatgccatct caaatgaaca gcacggcaga ttttgtttca tggagtgcta taatgacagg
     6841 gaggtctttc gtgcactgct taaaaagatt gaagactggg caaggcaaaa agggatgaaa
     6901 aaaattgtgg gtcctcttgg tttttccgat aaagatcccc agggtttcca gatagaagga
     6961 tttgagtatg aacaattcat taccagcccg aacaactccc cttatatggt cagcctaatt
     7021 gagggtgaag gctatgaaaa aaaggttgat cttgttgact accttgggga tatcccggaa
     7081 caattccctg ctgtttatgg aaggcttctt ggcaggaaca attcaaccgg agaattcagg
     7141 attattgagt tcagctccag aaagcagtta aagccgttta tccagcctgt ccttgagata
     7201 atgaatgaaa catttgatga gatctatgct tttgttcccc tcgacgacag tgagaagtcg
     7261 gaacttgctt caagatattt acccatcctt gatccccggt ttgtcaaagt tgcagaaagg
     7321 gacgggcaac tgattggttt cgctcttgcg attccggatg tgagcccggc aataaaggcc
     7381 tgcaggggaa aactgtttcc ttttggtttt attaaaatac ttcgggaatc aagacgtacc
     7441 gggaagcttc tgatgctcct cggagggata agacgagaac acaggggaaa aggcgttgat
     7501 gtccttatgg gagtaaagat cttgcaggaa gcaatagcaa gtaatatgaa aatcattgag
     7561 agtcatctgg ttctcgaaga aaatttcagg atgagaaaag agtatgagag aatcggctgc
     7621 aaggtgatca aaaaattcag gatctatcag aaagacctgt aatattctcc ttgctgcctg
     7681 aaatagccgg aacgcgtttt aaactacagc tccgaatcct ttccgatgaa aaccgtatga
     7741 ctgaccaaag gcacagacac gattgaatat aattgcgttc aatcatgtgt atttcttttc
     7801 cggagggagg ccagacaggc atccgatctt ttttaaaaaa atttatagga atgaaacaaa
     7861 tcacaagaag aaaatttatg gagaaggcga tgatcaccgg tgcaggggtg tctgtcatgc
     7921 cggtgatggc atcttcgggg gataataacc ttaaacaagt cggaaacact ccgccggtgg
     7981 atacggaact cccgggaatt cccgggaata ttataattca gccgccattg gcggacggtg
     8041 atgctcttca gcacgacttc agcaccatgc gtccattgag gatcaaggct ccggtcatcg
     8101 taacagtcgg ccaggatgag ggggatcttc gggggaagga taatattgta ctgcaggctg
     8161 cagtcgatta tgtggcaggc ctgggcggag gtacagtaca tattctgccg ggcacctata
     8221 taatgcggaa ttcccttttc ccgaaggcag gtgtgacgat caggggttcg ggcgaaaaaa
     8281 ccattctttg gaaggctcca agtgtaagca gcaaactgat ccgggaagct gactggtatg
     8341 aatactgtgt tcaggtggag aatcccgaag gattcacccc gggcggcggt ttggcactca
     8401 gtgccgataa aaaaaacgat caggaagtta gtctttttac tatcactgca ataaaagaca
     8461 atatactttt tctcgaccgc cggacggaaa aaaacttctg gccggttgat gaagcccggg
     8521 ccaccacgcg tttctcgata attcatgctc tgaatgtgaa tgatgtgaaa gttgaagaca
     8581 ttatcctcga cggcaatctt tccgcgaatg aaagggtgaa tgacaattac gccgcagcag
     8641 tttttattca atactgcgac aactggagct tcaggaacgt gacagcccga aattacaacg
     8701 gcgatggttt cagtttccag gtttgtgatg atgtccggtt tgaaaactgc cattccctga
     8761 acaattttga tcttggatat catcccggaa gcggttcaca gcgtcctgtc tttaaaaact
     8821 gtacggcaaa aggcaacagc cagggattct tctggtgctg gggcgtctgc tatgggatcg
     8881 ccgaaaattg cattgcttca gacaacagga aatacggggt gaatttcgga caccgcgaca
     8941 ctgataatat tctcaggaac tgcctggtag aaaataatca ggaaatcgga atcctgttcc
     9001 ggaaagaaga aaatgaattc cggacaggcg accgtaatat aattgaaaac tgcacaatca
     9061 ggaataacgg aacagctggc cccggaacag gaattgatat ccagtggaaa acgaatgaca
     9121 ttacaattcg tgactgttat tttgaaaaca cccggaacgg acctcagaag accgccataa
     9181 aaataagcgc cgatgctcag cgaataactg tggaaaataa taaattctca ggagatgcag
     9241 taaaaattga agatctgaga aaaaagagct gaacgttatt cttattcctg gtatttaaaa
     9301 agcagcaggg tgggatttcc gcttccgccc tcgtttgtta ttaccatatc cccgttactg
     9361 taaaaggcaa tgccttcggc attattgaag accgccgggt tcagtgtctc aatatgttca
     9421 atgtttccgt ccatgtcgga gataaaaagc aaataatctg aagatgaaag aataaaaagc
     9481 tttttggaca caggatttat gcctatcgct gaagtcatga aatcaatccg cggctccggt
     9541 tgtttccctt tctttttctc cttcagagga aggtttaact tgtttgcttt tgcaaaattc
     9601 ctgattgcct gaagatcaaa tatgaaaacc ggatcctgcg aaagtgttct ggttttaaga
     9661 tcaaaagcat aaatttccct cttatccttg tctgcagggc ccttcagggt ttttcctttt
     9721 ggtgctataa gcagccggtt gccgtccgga tcaaagcaga gcccttcatt gttccttgac
     9781 ggaatgtttg tgtagaaaac atcaaaattt aaatttcccg actcatggcc ggcgatctcg
     9841 aaaagtgccc cgtcacttct gagcacgtat attttacttc ctgcaatggc tatcccttca
     9901 taatcaccat tgatattaaa gtgcaattgt ctttctatct tgtttttctt taagtcataa
     9961 aaaaatacta ttccattctc gtcctggatg caggcaaaca aatccgattc cagatgggtt
    10021 atccccgata tttcgtgcaa aattgctgga agtataagga cggcatcagg tttactcaga
    10081 ttatatccca gggtataatt ccccgcattt tttacagggg cgaatccggg gaagatgaga
    10141 aatgcaagca ttgatgctgc tcgaaaaaat ttgatcatta acttgctgta ttttgtatat
    10201 ttatggataa tatcttcagc ttcggcagcg tttaataggc aattgtacaa aatatttatt
    10261 ataatacgat tgtggttgaa aaaaatgccg ggtcaggacg gttgtacgat acctgatcca
    10321 gaccccggca tcagagaaca gaagtttaat tgaaacctgc aaaatataat cttccggtga
    10381 ttgaatcaaa gcaacaaatt gtgcaagtac ttaccggctg aagtaaatca tatttaacaa
    10441 aatctggcaa tgtccgacaa tttcttcaat ctgaaccatt ccgatttttt cggtattctg
    10501 atgaggtttg caatcaacac gattttcctc atcatcctga tacgggtgat ctactttcgt
    10561 tattccagga aggagaagtt cctctttacc tttttcctta tgggaataac ggtctttttc
    10621 atcacttcta tgctgaaatc ggttttcatc gaattcggaa tggcgatagg gctgtttgcg
    10681 atctttgcca tcctgaggtt cagaacgagg aatttcagcc tgaaggatat gtcctacatt
    10741 ttcaccacaa taggaatatc agtaattaat tcattgaaac tgactggttt tccagtgctc
    10801 ggagtgatta ttttcaatat aatcatcatg gtttcggccg taattcttga agaattcact
    10861 gtgaagcata atattgtgag ccatactatc atttatgagg atctggattt gcttaagtcg
    10921 gcaaaaaaac agaagatcat cagggaatta tctgccctta caggaaagga tattacatgc
    10981 gtcaggatca gaaaaatcaa ttacaaagat aaagtggcgt ttctcgatat ctcatacagg
    11041 gattaaagtt ggatattatt gaaggatccg gcttccggat ttatcaggat taatttctgc
    11101 aggaacagaa ttatcactat attcaatggc attttaacct gaaaaaccgg agcagatgca
    11161 gattgaccgc aggagattca tacggtcatc agtgcttgca ggtgctggtt tagtactgtc
    11221 accgggcaag ggattgtgtg atgaaaatac ctctgactac tttggggttc atccctttat
    11281 tctgatgaac ccggatgccg ttttcataat gaaaacaaat gtcgacgtta agacaaattc
    11341 ctctgtcatc aaaaagacgg gtctggattt tggccgttca gtcttttgca ataccggcga
    11401 caaggagaac ggattcccca aaaccggcaa aatagtaatt aaacccaacc tgacatgccg
    11461 cgcaaggaat agctccgcat atacgatcga gcggtcgatg ggaattgtga ctgatgcatt
    11521 ttttgttgag ggcgttatag aaagtttaaa ggaactgggg ctttcatcca accagctttt
    11581 tatcagggaa gtcaattgcc cggatgattt ctcagacgga ggctatattt caatggctga
    11641 acgaaccgga atagacctgc agggaatcaa tacacctgtt cagaatctgg cgcctgagca
    11701 ggtttcatgg gttaatgtcc ccgggggtgt gtttttcaga agaattcctt acttatggcc
    11761 ggttaatgcc ccgggtactt tcctgatcaa tatagccaag tttaaaactc atggaatggg
    11821 aatgaccctg tgtgcaaaaa acctgcaggg caccatagca gcaaactacc aggcccattg
    11881 cagtgcttac ggcaggagta tttccggggt gagtgcctct gatctgaatc ctgcagctgc
    11941 ctccgacatt cttgccaatt ataacaggca cagggttgcc ggaattccgc gatgggataa
    12001 acccggcagt gacggcggct tatggcagga gatatgggca acccggtgtc ttgataataa
    12061 ttcagtgaca ttcgcaggct tgcatattat agagggaata tatggccggg acggtgattt
    12121 tatgacggga ccggcacaga atggcctggc aaccgattat atgacaaaca ttataatctt
    12181 tgggcggaac cagttccatg tagataacat aggtcattat cttgggggtc atgaaccggg
    12241 aaatttcggg ctctttcacc ttgcactcga gagaggcatg gcaaagacaa taaatcccgg
    12301 ggaaataccc ctgtatgaat gggatattca tacaggtgcc agaccagtca gtctcgaaag
    12361 ccagcaacgt ttcaggttaa aaacctatta tcttcaaagg aattacaacg ggcaggatga
    12421 accttactgg caccttgtga atgaacccta tgaatatgac tccgcaggag tgaatgaccc
    12481 cggtttcagc agaagcggct tccaccttca gcagaatttt cccaatccgg ttaataccgt
    12541 tacatcaatc cctttcaggc tgccttcagg gggaaatgca cgccttgaaa ttctcaatac
    12601 atccggtaag ctgatcgaag tgattgttaa caggaatctt gaaggcggaa cccatactgt
    12661 attatgggat agcagaacag tcccctcagg atcatatatt tgcaggatcc atttcaacag
    12721 catgaatagc acaatctgga tgatagtcat ccattagtgc ctgatccgct ttactccagt
    12781 ttaaaccggg aagttaatca tatgcaatta cctccagtcc gacgtttgat ttggaaaaca
    12841 attgcctatc tttggtttat taacctgatt actaatccgg aaaaaatagg cgctatgaga
    12901 ttcaattcaa ttcctttcat tttattgctt cttgctggca tattgttctg caattcctgc
    12961 aaccggacgc cctactatgg gccggatatc tacgttcccg ataccacctt caaggttgta
    13021 ggctatctgt cgacgggaaa ttttgacaaa attgacgcgc ttgagcttga caagcttact
    13081 tacctgaaca tcgcattcgc caaccccgat gctgatggaa accttgttgc cgagctcaat
    13141 gccgacataa aacctgtggt acaaaaaggg cacgacctga atctgaaagt ttttatttcc
    13201 cttgcgggag gaggcagacc cgacaccgtg atctggaaca acctgctgaa acctgaaaac
    13261 agatcagcat tcataaagaa gctggtagca tatgttgaag aaaataatct cgatggcgta
    13321 gatgtcgaca ttgaaggaaa cctgcttcct tatcttgaca ataattacaa tccttttgtt
    13381 cttgaattgc gcaaagccct tcatgccaag ggaaaaggaa taaccgccgc cctcggggct
    13441 gtcggctttc atgaagctgt tggccaggag gcgcttgaag cctacgattt tataaatgta
    13501 atggtttatg ataaaaccgg gatatggaga ccgcatgata tcggcccgca ttcaccatat
    13561 tcctatgcaa ctgatgcaat taaattctgg acgatcgaca ggaagatccc gcctgaacgc
    13621 atcaccctgg gggtgccgtt ctatggtttt gattttactc ctcctgcaag atatatttcc
    13681 tacgcggaaa taatcaggga aaacaaggga tatgcctatc atgattccgt cggaaccaga
    13741 tactactata atggtatccc aacaatggtg aagaaaacag aacttgcaaa gaaaactctt
    13801 ggcggtgtaa tgatctggga gattgcctat gatacgacag gtgatctttc attgatgaaa
    13861 gcgttgtacc agacactcga ggcaggaaac tgccaggtaa gaacattttt cagggatgag
    13921 gacggtgatg gtttcggaga tatgtccaga ccgattcagg catgtgaagc cccggaaggc
    13981 tatgtggcaa acatggacga cagggatgat aaaaaccccc gggtgcatta aactgaataa
    14041 agatacttcc ggatccacag gatcagtcac cggataggtt atatgtatca tatggcttgc
    14101 taccagtatg tattccttcc tgaagcatgt attactatgg ttttcaatat acagctttcc
    14161 tgcccggagg tcgtcagaca ggtatccgat ctttaataaa taaattatac aaggatgaaa
    14221 atatttaact atgaattgtg gtttgtaaca ggcagtcagt cactttatgg tgaagaaacc
    14281 ctgaaacagg ttgcggaaga ttcccggaca attgcggcag ctctcgataa taatcatctg
    14341 atgccggcaa ggattgtttt taagcctgtg cttacaacat cagacgcaat cacaaaacta
    14401 tgcctcgagg cgaacagtga ccagttgtgc gccggaataa taacatggat gcatacgttc
    14461 tcaccggcaa aaatgtggat tgccggactg agccggctcc ataagccaat gctgcatttt
    14521 catacccagt tcaaccgcga catcccatgg aaggagattg acatgaattt tatgaatctc
    14581 aatcagtctg cccatggcga ccgcgaattc ggatacatat gctcacgcct gaggctgaac
    14641 aggaaggttg taacgggtca ttggcaggac aacagtgtaa tcggtgaaat agcagcctgg
    14701 gtcagatcgg cagttgcctt tacagaagcc ggatctctga aggttgcaag gtttggggat
    14761 aatatgaggg atgtggcggt aacagaaggc aacaaggtat cggcacagct tgaacttggc
    14821 tacgaggtcc acggattcgg ggttggcgat ctggtcagtg aggtgaatgc tgtttcagcc
    14881 acagcggtta aatcgcttgt tgaacaatac ggggatgaat acatggtgca gcccgaagtc
    14941 ctcaattcac caccggtaag ggaggctgca cgcattgagc ttggcatgcg gaacttcctt
    15001 gaaaagggga gtttcaaagc ttttaccaca acattcgagg atctccatgg cctggcccag
    15061 cttcccggcc tggcggtgca gcggctcatg gccgccggct atggctttgg agccgaaggc
    15121 gactggaaaa cagccgctct cgtgcggtgc atgaaagtaa tggctgccgg gcttgaagga
    15181 ggaacttcct tcatggaaga ctatacctac catctcgacc cggggaatcc ggctgtcctt
    15241 ggagctcata tgctggaagt atgcccttca ctggcagacg gaaaaccctc gttgcaggtc
    15301 catcctctcg gcatcggcgg gaaaaatgat ccggcaaggc tcgtttttaa aacaagaaca
    15361 ggggaggctc tgaatgcttc acttatcgac ctgggtgaca gattcaggct ggtagtgaac
    15421 ccggtcgaag ttattgactg tccggacatg ccaaaacttc cggttgccag tgtattatgg
    15481 aaaccccgac ccaacctgaa aactgcagca accgcatgga tctacgcggg aggcgctcac
    15541 catacaggat tttcacaggc actgaacacc gggcatcttg ttaactttgc cgaaatagcc
    15601 ggtattgaat gtattgtgat cgatgataaa tgcagagtgg acgaattcaa aaaagaactt
    15661 cgctggaacg atttgtttta tcatatctga agaaatgtta cagaatctta aggaagaagt
    15721 atatcgggcc aatctgagcc tggttgaaca taaactggta aaactgactt ttggaaatgc
    15781 cagtggaatc gaccggaaga ccggattcat ggtaattaaa ccgtcaggga tttccatgag
    15841 ggactcaggg cccgatgata tggttgttac cgatctgtcc tgcaatgttg tggaagggga
    15901 tctgctccct tcaaccgatg ctccgaccca cgcagcactt tataaagcat tcggcacaat
    15961 cggcgggatc gttcataccc attctgcaag tgcaacagcc tgggctcagg cctgcagacc
    16021 gattccctgc ctcggaacca ctcatgccga ttatttccat ggaaacgtac ctgttacccg
    16081 ttcgcttacc agggaggaaa ttgccggaaa ttatgaatat aatactgccc tggtgatagt
    16141 agaatgtttc agggagatgg accctctcag gatacctgcc gtactggtcg cttcccacgg
    16201 accattcacc tggggcgggg atgtgacaca agctgtgctt catgctatca gtcttgaaga
    16261 aattgcccgt actgctgctt tgaccctttc aatagcccct gtaggaccga ttgatcagac
    16321 actccttgat aagcactttt tcaggaaaca cgggcagggt gcatattacg gacagaaaaa
    16381 atagccggca ttcggtttct gccatatatt cccggttcgg atttttaggc ggattgagtt
    16441 attacccgta gcgggtgaca tgcatgaaag tgagaatatt taatgtagct gctctcaatc
    16501 tcttaatttc tcccgtaact ttacaagaaa aagatacatg tcatgaccaa attgtcagta
    16561 ttgtggaaga taaggctggt tatagtgatg attatcattg ctcagctgac tgtttcctgc
    16621 agcaaaagcg ggcagaagga tgatgaaacg cctcctgttt tgtcagttac cccggttgat
    16681 atcacaacag ttactcatat cataccattt ggtgccgatc tttcccctca gcaaaagaat
    16741 ccggcgtttg agtattatgt cagcaattca tctgtgcagg taagatcagt atgtgccggt
    16801 tatgttgaag atatcagact gaatgacaac tttcccgatt acgaggtatg gattaagaca
    16861 tcatccaatt ctgtttacaa attaatttac gatcatatcg ttgatcttaa tgtggtaaaa
    16921 ggcaataaag ttgatgccgg tactgttctc ggcaaagttg gaaaaggcaa caggaccgaa
    16981 cttcagatca acaagagtga caggaacatg ggtgaattat cttattgccc tttcgatttc
    17041 gccaccgatg attttatcag cagccataaa caatttaccg atacatggtg tcttaccgga
    17101 actgtcaggc cgtaaaattc atctttccgg gctgatatcg ccccaatgac tccgggttgg
    17161 tctccagcat tgccctcttc atattcaggt tgacaatcca aatttaatcc tcaatcctca
    17221 aaaaatcacc aacttttttt gaccgcatta ccattttttg agcgttgcac tgcagaaaac
    17281 aggaagacac ctgacataag gcaaagaaaa caggttccgg gaagactgct gttccgttat
    17341 ttaatttccc cggttcagaa tttctgcagc aattccttca cggcatcctt gtggaccatg
    17401 aaatccatca ttttaagttt tacatagtct tctgaaaaga aataatagcc gaattcagcg
    17461 gcatcaggat cgttgttccg ggaaccggag ccggagtcct ttaccaggta ccagtctttg
    17521 ccgtcttttt ccaggtatcc tacaagatgc atcccgtgat cgtcggtggt tgtacggttg
    17581 gagaacctga actgacgggc gtcatcattg atatatgctg aaggaatgtc aaaatccgga
    17641 atgacggcgc attgggtcac cctgtcgaaa cccggttccg aaacgtcgcc tccaatgctg
    17701 acggtgtatc ccttccttac ggcatttttt agcgccgtca tgaaaacatc gagaggaaca
    17761 ttgtgataat cactgttgtg ccaccagttg tcgggtacct ggtattcaac cttcttgtag
    17821 aatggttcct gggtatagga aagtatgtcg acataatctc cgggattaat cttcaggata
    17881 tccctcagat attgaacagg agtcattttt tccccgttaa ctgttatttc tgcaggcggt
    17941 tcgcccatgt aatgattcat tattgatttg attgtagcca gggcagcatc ctcatcccat
    18001 gaagaagatt ccttcaccga ctgcaggtaa ctctgcatct cttccctcat cctgtcggta
    18061 ttgtggaatt tccggccatt cagcaggccg gtgtagtctt catacggaac aattccgtat
    18121 ttgctccaca tccttgttac ggcattggct tctgatcctt catcaaacaa tgaattgccc
    18181 ctttccctta tgaagcgcct ggctttttca acatattccc agtaaacagt atacatttcc
    18241 gataatttta ccttcatttt atggatccgg tatatttccg attccaggaa tgaggttgta
    18301 cagaaacacc agcatgtgct tgtactcccc tgtgaaacgg gaggattatg ccattgctga
    18361 ttcctgtaca ttgaaaattt gttgggcagt gatgagcttg acagatcgac tgcaaacatt
    18421 ttcattggcc tggcaggctc ggctctttcc ctggcctccc tgtcatcgcg caggatcgag
    18481 ttctgataaa accccggagg cagtgttctg aacacagcct tatccttgtt ttgtgcatta
    18541 atgacaatgg gtattgcaaa cattattgat gcaatcgcgc agaataattt tcttttcatg
    18601 tctttgtgtt ttttcctgtt cggatgtttg aatctacaaa ttacaaaaaa aattacggtt
    18661 aatatgctgt tacaatcatt tagcggcctg tgaaggaatg ttttattttc atgtttcagt
    18721 atacaatcac gttttgaaaa gtcttaaata actgacatat gacagacttt atattgattt
    18781 aagtctatgc ttaagatgat attgatcaat tataatgttg acaaatatca gtttatttat
    18841 tgcgaccatt ttgtcggcac cctgttccag cgatgttttt ccagttcgtt catgagtttt
    18901 gcaggcaagg ggcctttttc gcttgctgaa agattggatc tcacatggcc gggttttctc
    18961 attcccggaa tgattgtagt cactgtcggt tccccgagta tgaatcggag agccatctcc
    19021 ggcattgaca tgccttcagg tagcagaggt ttgagtgcat cggctctttc aacgctgggg
    19081 atcagatttt cagggacaaa ataagtattt ctccagtctc cctcgggcca tgtggttgtt
    19141 ttggtcaggg ttcctgtaag ggtgccttca tcgaaaggga cccgggcaat caccgcgaca
    19201 ttccttttcc ggcaggccgg gaagagttca tccttagggt tctggtcgaa gatgttgtag
    19261 atcacctgaa cggaatcgat cagtccgctt tccactgccc ggatcccgtt ccagggttcc
    19321 cagcggttca ggcttatccc tatggcatga aaaagtccct gtgatttaag gtctgacatt
    19381 tttttgattc cgcggtcatc gtcgagccag ctttcttccc aggtgtggaa ctgcatcaga
    19441 tcaaaggagt caagtcctgc gtttttcagg ctttttgaaa catattcctc aatatggtcg
    19501 ggcgggaagc agtcgtcgag gctgaattcc cgggtgctgg gccattggaa attctttgga
    19561 ggcatttttg ttgcggtgta gattttttta tccctgtttg ccctgatgag ttctcccagg
    19621 agatgttcac tttttcccgc accgtatccc caggcagtgt cgaagaagtt gcatcccaga
    19681 tcggcagcca gttgcagcga cctgaatgat tcctcatcgt ccgagcctgt ccatcctgcc
    19741 attccccaca ttccgtagcc aatttcactg atcctccagc ctgtttttcc gaatagtctg
    19801 tatttcatgg ttgatgtgtg ttttattcag ggtcagttat caggccctta atttaaaaaa
    19861 gatcaggatt cgatattcaa aagtcagata atgatttcat aattaattta tcagcgccag
    19921 gagtcaggca attcgccgcc atgcggttac agacatgtca ccgctgcgcg gtttgggaat
    19981 tattatgaat gccctgatcc taattcgcgg agaacaatcc ggaatatact tgtttcatgg
    20041 tttcggtttt tgctgggttc tctgcgactg cattggcggt gaacatcatt attcccgtga
    20101 ccggtacggc tgaaccgtaa ttcagcacct tccggaattc ctcctgcgtt attgcgcggg
    20161 gatcatcata agcctgcact atgggccaga ccttcggaaa tgctcccggg ctgatgtcca
    20221 gccttctgca gaaccattcg atgttttccc tgacccattc cggctttctg cccatccggc
    20281 catgatatac catcggtgaa aaaacatctg tcagatcttt caacagatca taatccagac
    20341 ccaggatccg gactcttgca ccattgaatt ccccgtcttc ccatggacag tgaaagatac
    20401 cgagcagcgc acccggcctt tcttccttta caatctccct tgtctccctg acccagtccg
    20461 caattaccga acacctccat tcacgccacc cggcttcgtg catttttagt atccattcgg
    20521 ccttttccgg ggtgctcatg ccgggaaccc tgataccggt tgcagtttca aatgagctta
    20581 tgcagttatc acaaaaacag gtttctggaa ggataggctc cggctcttca aactgtgcgt
    20641 gccagtgaag ataatccatc catatcccgt ctagttcaaa tctacccacc agctgccgca
    20701 gctcgttcat cctgaactgc ctgaatccca gatcagtagg acaaactccc atgaaccatg
    20761 aagccggttc aactctctta ccatcactgt caatcgccca ggcctccgga tgcttgtcaa
    20821 catatccggc gccgttgaga accggaaatt cggcataaac tttcaaacct tccttccctg
    20881 cacgctccat gatcctgtca ttaatggcct tccagttcat aaaaaccgaa ttcacattga
    20941 gccggttcag ttctattccc ctgttccaga aagcatccgg actcccgtaa attcccctgg
    21001 cctgaaagtg accggttttc cccggcggtt cagtctttgt cagggtgttg acaggagaaa
    21061 aactacaaat caaaatggtt atcaatgcaa aaattgaaag aagacttctc attgcaggga
    21121 tattaaggta ttatttaata atgcaatata ggaatttgcg tcttagctgc atattttaaa
    21181 gatgcatctg tttggatgcc gtaaaaagac attgtgatcc aaaaccgact ggccccggat
    21241 tatgacagat gagcgggatc tcctgtccat tatgaatagt ttccggcgat ttgggcgcaa
    21301 tgaatattta tttgtaattt taataccgtc aggaattacc tccggccgtt agccatagaa
    21361 tcccgattga tgccggcaag aataaggaac agggcggccg ggcagaaatc cctgttcccg
    21421 catcctgctg aacccaggag cctgccgggg aaaaaagatc ggttatgatc cgggatgaaa
    21481 caaacaacac gaaatggaga aggtccttta taagatatat gtaaggggac gggttcaggg
    21541 agttggcttc agatacagtg ccgtgagagc tgcaaatcac catggcatta caggttttgt
    21601 aaaaaatatg ccggatggaa gtgtttatat ggaagccgaa ggaacggaaa aggaacttct
    21661 ggattttatt gaatggtgca gaaatggtcc gggccatgtg ggatcgctga cgattgatcc
    21721 ttatcccccg cgtaattatg tggatttcag aattgtattt taaattcagc catatcttgc
    21781 ctgatcagtc aatcttattc aaggcggaaa taattttatt taataaatca actctcccat
    21841 gaagaaatat ctgatattgg ttatgtcagt gtttatcaac ctgcccgttt catcacagaa
    21901 atatgaaatc cgttctcctg acggaagtct gacagccgga attgagataa gtaatggaat
    21961 tgatttgtcc atttttaaag gaagccttcc cgtccttaaa ctggaaaata tcgggctcgt
    22021 ggcttcggac agtgaaaatc cgttcagtga gtttaaagtg caaaagactg tcagaaggag
    22081 ggttaacgag gttgtcagac ctgaaataag ggaaaaggca tcagaactta tcaattccta
    22141 taatgagctt gagatccgat tcaggagtaa gtttgctgtt acattcagga tgttcgatga
    22201 gggtctggcg tacaggtttt cgacctcatt gaagaatgaa ctgaccattt acagggagaa
    22261 tcttgagctt tgttttgaag atggcgattc ggtcaggttt cagtcgtccg ggactttcaa
    22321 ttctgcctac gaaacacctt atgaacacga aaagccggcc ggaatcggag atggtaagct
    22381 gtgtaatctg ccatttcttg tggagaagaa agacggaacg tttattatgg tcacggaagc
    22441 cgacctatac aactatccgg gattatggct gaaagggacg gggcagccac ggttgtcggc
    22501 agtgaatccg ccatgtccga aaacattgac cgcaaccggt tctccctata acctgggcca
    22561 ggtcgaagaa gcttacggtt acattgccag ggtggcaggt acaaggaatt tcccatggcg
    22621 gatctttgcg gttgccggtg atgaggcgga gctagtttca aataatatga cctatctgct
    22681 tgcctctcca tgcgccataa gtgatgtttc atggataaaa cccggagttg tgatgttcga
    22741 ctggtgggcg aaacacaata tttacggagt cggtttcaga tcggggatca atactgaaac
    22801 agccaggtat tttattgatt tctgtgcaaa acatggattc aggtattttc tttttgatga
    22861 cggatggagt ccgggggaag atttgctgaa aactgtcccc ggattggata tcagggaagt
    22921 tactgcctat ggcagggaaa aaggggttga tataatgctc tgggtaatct ggcacacgct
    22981 tgggatgcag tgggatgaag cttttaacca gtttgaaaaa tgggggataa agggcatcaa
    23041 ggtggatttc atgaaccgtg acgatcagcc gatgattgaa ttctacgagg ctgttgccgc
    23101 tgaagcagca aagcgaaaaa tggtggtgga ttttcacggg gcctgcaaac ctgacggttt
    23161 acggcgtaag tatcccaatg tcctgacccg ggaagcactt attgaatttg aattcaacgg
    23221 ctggacaagc tatgatgatc cagttcatca caatcttttg ccctacataa ggatgtttac
    23281 cggcccgatg gattatattc ctgccacaat gcgcaactcc acgagagaga atttccgtcc
    23341 tgtgggagat tacccgatgg ggcagggaac ccgtgctcat gccatggcat tgtttgtgat
    23401 actgaacagc ccgatgacaa tgctgcctga ttcgccttcc gattattacc gtgagcagga
    23461 atgtaccggc tttcttgccg gaattccggt tgtctgggat gagacacgcc tgcttgaggg
    23521 gaaaatcgga aagtatacag tgcttgcccg cagatcgggc agggaatggt acctgggagc
    23581 catcacagac tgggacaaac gcacaatcag tctgaaaact gatttccttg aaccgggacg
    23641 gtactggctt gaggctgtcg aagacggcat aaacgcggat aaaagggctg aagattacat
    23701 aaaagtcaga aaggaatttc aggcaggcga ttcgttggta gtgaatcttg cgcccggcgg
    23761 aggctgggtt gcaaggatta tgccggtaaa tcagaacaca gcctccggtc gcggaaatta
    23821 atcaccgagt tacgccgaac ttccatgcat cgtaaccctg cggtcctttc cagtacttac
    23881 ttaagctcct gttcggcttt tcttatgttc cagccgtcaa ttgcaagcac cggtatttca
    23941 cctcccagat cttgatccga aaattccact ttgacactgc gtttctcctt tggcagaagc
    24001 gcaaaatagt tgtcttccca aaaaaccggc aatacaaggt ctcccgattc gcgtctgatg
    24061 atcttcgggt tcacagagaa agcaagaaag tccgaaggat tctcaatctc aataactgcc
    24121 ctgtacctcc cgttctcttt aagtaccggg gagacggagc agctgagttc aacctcagga
    24181 agcctgttca atgcagtgaa atccgcattc tcatccccct ttgatgaaag ccagtagaaa
    24241 tttgaagaca gttctctgcc tgaatcatcg ttcaggatga gttttaggaa tgtaattccg
    24301 cttccgggcc tgggcttctc caggaacatg actttgacac tctcatctgc ctttaccgca
    24361 accggcactt cttctgagtg aatttcattc atcttcatat catagagctt tgcggatgcc
    24421 ttcaatcctg agaaatcacg gtacagtcca ttgacgaccc ttacagagtg atcgtcgtac
    24481 gaatactgga tatgaaggtt ctcgcaggct ttttttatcc cgtaaaaagc tccgttcggg
    24541 gcaaggaagt agtcatagag ttgccagtac attgtgggcc atgctgaatt atacatccag
    24601 tagataatgc cgctggattt gtacttgttg ccggcatagg cttcgaacat tgccctgctt
    24661 gcttcatact ggagaacctg cgatttcatg ctgtattcat tgaggttttc cggtttgccg
    24721 tagcgtgaaa agagagcttt acgggcggca gggaaaaaag ctttgttgag cctgatgttc
    24781 cacgagtcgc ttataggcca gagatcctgt tccggcatca ttttccgcat gctttcaacc
    24841 gggggtatct gttcccctga cggcccggct tcagtgttga attcaagctt gccgtaccag
    24901 tatgaagggg gcatgtagga ataaacatcg ggataaggtc ccatccatac accggtattt
    24961 ccagttaccg tgctcggatc ctgtgttgct gatgattgga acggtcgtgt ttggtcaagc
    25021 tcgttgagca cgttcaggta catcttctcg atcttttccg tcggatgcat atcgcttccg
    25081 tacagccagt cgaataccga agggtggttt ctcagccgga ggatcaggtc ttcccagctt
    25141 ttttgtgcga cgatctccat ttcaggggtc catctgtccc acatttccca tgagctgcag
    25201 caacaccagc cgaccatcag gagtatccct tcctcatcgc atttttcgaa caggtaatcg
    25261 gatcccctgg gcgcctcaag tcgcaatgta ttcaatccca tgtgctttgc ataccggata
    25321 tctgcatcta ttctctcatc cgagggtcgg agaagcatgt cttccacata gcctccacct
    25381 ctgatcacga tgtttttccc gtttatctgg aacactctgg tgtgcctgcc gtcaaaatca
    25441 ttcatccatg aagatatttc gcgtatgccg aaacgaacct ttttaatgtc ggatacagag
    25501 cctttggttt caaaggtcag gttgaggttg tacaggttct gggggccgac
//
LOCUS       DAOOYG010000028        25271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1759_length_25271_cov_6.827054, whole genome shotgun sequence.
ACCESSION   DAOOYG010000028 DAOOYG010000000
VERSION     DAOOYG010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25271
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1759_length_25271_cov_6.827054"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PK288_04245"
     CDS             <1..267
                     /locus_tag="PK288_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPM18162.1"
                     /translation="SRVGGVVKSKDYSSFDVGIAVNSMILQAAAEGIGSCIIGWFDEK
                     EVKRILGIPSSRRVDLMITLGYPEGERRNKKRKPGDVTVSYNRY"
     gene            complement(311..1504)
                     /locus_tag="PK288_04250"
     CDS             complement(311..1504)
                     /locus_tag="PK288_04250"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPM18163.1"
                     /translation="MEYLSDRIRALSDSQTLAMAQKSRELKAKGLDVISLSLGEPDFN
                     TPDYIKEAAKKSIDDNYTKYPPVPGYDDLRKAISRKFREENGLEYTPDQIIVSAGGKH
                     SLANVMLCIINPGDEVIIPAPYWVSYYDQVVLAGGKPVIVEAEIENDFKVSPGQIEKA
                     VTEKTRLLLFNSPSNPTGMVYTGNEMEAIARVVERHEGLFVMSDEIYEHIIFEGEHVS
                     MASFDFIYDRVITVNGVSKGYAMTGWRIGYLGAPLWIAKACNKLQGQITSGVCSIAQR
                     AALAALEGQDDSRRKMKEAFHRRRDLICGMLKEIPGLKVSVPQGAFYVMPDISYYLGK
                     SDGSVKINTSDDLALFLLDKARIAVVGGDAFGAPSCIRISYANSDELLAEAVRRMREA
                     LELLK"
     gene            1842..2363
                     /locus_tag="PK288_04255"
     CDS             1842..2363
                     /locus_tag="PK288_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18164.1"
                     /translation="METLYEILKIVLPAAIVLLTAWVILRNMIRNDQNKRRHELILQG
                     ARTVTPIRLQAYERIVLFLERISMESLLIRISTPDMTTGQLQTALLSAIRNEFEHNLS
                     QQIYMSPQAWEIVKTARSNTIKIINSEAEKIKPTAPAIELSKRLLEKVMELDKEPTRA
                     AIEYIKNEVSEII"
     gene            complement(2456..4501)
                     /gene="htpG"
                     /locus_tag="PK288_04260"
     CDS             complement(2456..4501)
                     /gene="htpG"
                     /locus_tag="PK288_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008668996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPM18165.1"
                     /translation="MQTGKIGVTTENIFPIIKKFLYSDHEIFLRELISNAVDATQKLK
                     TIASAGDFKGEVGDLTIRVSVDKKKKTIRVSDSGVGMTADEIDKYLNQIAFSSANDFL
                     DKYKDQANSIIGHFGLGFYSSFMVSDKVEVISRSWNEPAQAVKWVCDGSPEYQIAETE
                     KENRGTDVILYIDKDSKEFLEEDRIEQLLKKYCKFLPVEIAFGKEKEWKDGKYVDTEK
                     DKIINNTRPAWTLKPADLKDEDYRNFYRELYPMGEEPLFNIHLNVDYPFKLTGILYFP
                     KIKSNIEIQKNKIQLYCNQVFVTDSVEGIVPEFLTLLHGVLDSPDIPLNVSRSYLQSD
                     TNVKKISAHITKKVADRLNEIFRKDREEFEKKWDSLKLFIDYGMITEEKFYEKASKFA
                     LFKNTEDKFFTFDEYEKLIKENQTDRNKTLVYLYSTNKTDQYTYIEKARNKGYDILHL
                     DGQLDVHLINHLETKFKDSRFVRVDSDVIDKLIQKEDQKESKLTKDRQDDIRGVFNLK
                     MTSKEVFNVVTETLDENDAPVIITQSEFMRRMKEMSQIGGGMNFYGELPDSYNIVVNL
                     NHPLILRIADDLQEKAGERLTENNSERNRLKDEISFMENEHKKKKAEEISQFEKDDLE
                     KLRKELETCENARKDILGSYGTENKVVKQLIDLALLANNMLRGEELGTFVRRSVELL"
     gene            complement(4587..5402)
                     /locus_tag="PK288_04265"
     CDS             complement(4587..5402)
                     /locus_tag="PK288_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18166.1"
                     /translation="MKFYAAILIFFSAVISNLAVVRENNPEDELSRLFGRIASGIPDQ
                     QKILASDSIRLIIDSYVYSDSIFSHEFSNLKYLGQITSKDSRMKIITWNLPLKENPGR
                     YYCYFINRRKEGNRILRLSANYNPDSIRTDTSYFQEDWYGALYYDMRPVRKGNEVFWM
                     ILGIDYGNPAITRKVIDVLSFDDADRPVFGKKWFDTGTEVKYREVLEYSFTAVISLRF
                     MSDKMIVFDHLVPISPEYLNNREFYGPDYSYDAYRFDKGTWHLRLDVDARNKE"
     gene            complement(5399..6604)
                     /locus_tag="PK288_04270"
     CDS             complement(5399..6604)
                     /locus_tag="PK288_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPM18167.1"
                     /translation="MLSNRRLFLDFLGKTSEMPMLLEIEKASGIYLYAPDGKRYIDLI
                     SGVSVSNTGHCNPEVVDAVKEQADKYMHLMVYGEFIQSPQVKYAAKLASLLPEPLECC
                     YFVNSGSEAVEGSIKLAKKLTRKSKIISFRNSYHGSTLGALSVQGNEKFRNPFRPLVP
                     DIYIADFNNAESLEIIDDHTACVIVEPIQGEAGIIVPVNDFLKKLRQKCDESGALLIF
                     DEIQTGFGRTGTMFALERYGVVPDILVLAKALGGGMPLGAFISSRENMSSLASDPALS
                     HITTFGGHPVCCAAGLASLDVIIGQKLVGSCAFKSDLFRKLLVHPLITEIRGEGLMLA
                     VQLASPRHVSYAVANAPSFGLILDYFLFCDSAFRIAPPLIISGEEISIACRQILDLLD
                     DTMANTRKE"
     gene            complement(6619..7326)
                     /locus_tag="PK288_04275"
     CDS             complement(6619..7326)
                     /locus_tag="PK288_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM18168.1"
                     /translation="MNCIIVDDDKLSCKVLEGYVTKYSSLNLIGVFYDAISARNEIIK
                     HKDIDLILLDIKMPEMDGFDFIGSLDHPPNIIIVSGESEFALRAFDFNVVDFLLKPVT
                     YSRFCKAIDKTIRYFSPKEYSPGEDEEIFIKKGSTLVKLKLKDIVFVEALENYVTLNT
                     KTDKYTIHFTMKAIESQLPSSVFLRVHRSFIINKSMIKTIKENSLDITIGNTVKNIPV
                     GKSFKDSLLNDINVMAK"
     gene            complement(7650..7919)
                     /locus_tag="PK288_04280"
     CDS             complement(7650..7919)
                     /locus_tag="PK288_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPM18169.1"
                     /translation="MTKKELVNAIANDAGLSIKDSGKALNAFVAAFGKAMKKGERIQL
                     PGMGTFTVNKRSARVVRNPRTGAKLNVPAKKVVKFKAAPALNKGL"
     gene            8334..8425
                     /locus_tag="PK288_04285"
     tRNA            8334..8425
                     /locus_tag="PK288_04285"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8368..8370,aa:Ser,seq:tga)
     gene            complement(9074..9457)
                     /locus_tag="PK288_04290"
     CDS             complement(9074..9457)
                     /locus_tag="PK288_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPM18170.1"
                     /translation="MKFLERYQNEIFALLRIVTGLLFIPHGIQKIGGFLSGSMPPDNV
                     MMIIAAVIEAVGGLLILIGWQTRITAFIASGEMAAAYFIGHSSAGLLPINNHGELAVL
                     FSFLFLFIASYGAGKWSVDNCRKPA"
     gene            complement(9676..10581)
                     /locus_tag="PK288_04295"
     CDS             complement(9676..10581)
                     /locus_tag="PK288_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HPM18171.1"
                     /translation="MKIIRLVETHFWVVMIAGIILGLWPPFEVNTSPVVQKILLAMML
                     FVAFMKIDAIEILENMKNLRLMSWITFVYMIVIPLGFYFLSGIFDHEFAVGILLLTAM
                     PAGVSSPVLTDIAKGNIPLSMSLAILTQLIAPFTVPLLFRLVHIRLVDIDEFLMLRDI
                     VILVFVPMILSQLTKKLLPGVVAGLHDLLTSFNVLIIFSFVFVTISSQREVILGNPAG
                     LVWKLAALYLLFILLHIIGYMIIPRENRESRIAVAIGSAYMNNGLAIVLAVSYFSPAV
                     LVLMVLSQIPWNTLLAPFKKVMERI"
     gene            10805..11764
                     /locus_tag="PK288_04300"
     CDS             10805..11764
                     /locus_tag="PK288_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HPM18172.1"
                     /translation="MKIIVLDGYALNPGDLSWEKLHGLGDVKIYDRTPAELVLKRAEG
                     AEILLTNKTILEAGTLQLLPKLRYVGVLATGYNVIDTAEARRLGITVTNIPAYSTASV
                     AQHTFALILELYLHIQRYSDSVRAGKWSKSKDFTYRDLPLRELAGKTLGIIGFGNIGR
                     KVCEIAMAFGMKVVAYSPSKSNAPQYDGFEWDEPDGLLCRSDIVTLHCPLTPETAGMI
                     SKTKLKLMKKSAFLINTSRGPLIVEEDLADALNTGIIAGAGLDVLALEPPPSDNPLLK
                     AKNCIITPHIAWATIEARTRLMEIAVENIRAFIAGSPENVVNR"
     gene            12560..13663
                     /locus_tag="PK288_04305"
     CDS             12560..13663
                     /locus_tag="PK288_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPM18173.1"
                     /translation="MKKPGTFLITSAIVILLFGQGIIPVNELMVRAVTQTTAATAAQS
                     KPAPAQKQTAPAQKQTAPAQKQATPAQKQATPVQKQTTPAQKQAAPAQKQTTPAQKQA
                     PPAQKQTAPAQKQAPPAQKQTTPAQKQTTPAQKQTPPAQKQTTPAQKQATPAQKQAAP
                     TQKKSPATEPGTKTAIKTPETGIVRIGSQVWAATNLNVVTFRNGDTIPEARTNEEWVA
                     AGKSEKPAWCYYNNDPKNGSKYGKLYNWFAINDPRGLAPEGWSLPDASDWAQLAGYLG
                     GAGTAGNKLKSSSGWADGYIGSNESGFSGLPGGYRVENGNFHNLGSIATWWSITEGKP
                     GTAIDHYIGQRGSLERSHNPKQVGESVRCIRKQ"
     gene            13797..14603
                     /gene="coaW"
                     /locus_tag="PK288_04310"
     CDS             13797..14603
                     /gene="coaW"
                     /locus_tag="PK288_04310"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009842.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HPM18174.1"
                     /translation="MVIGIDIGSTTTKVAAVNSGKKGIIMVRTKAFDAITSATGAFGK
                     LLVENNLNLANIEKINITGVGASRIPGNLFGIATTRIDEMVAIGTGGKYLAKKDRIII
                     SNIGTGTAIIQVDGSAITHLGGTGVGGGTITGLARQLLNISDFDTIMNYAAKGNINNI
                     DLQIGDIAEGNIGFLNREYTASNFGKMLDTATREDIAAGILNLTYQVIGMVSVFAARS
                     KNNDTVVVTGNGSNNILGQKILASISSMYNIQFEFPANAEFATAIGAAQS"
     gene            14752..14826
                     /locus_tag="PK288_04315"
     tRNA            14752..14826
                     /locus_tag="PK288_04315"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14787..14789,aa:Val,seq:cac)
     gene            complement(14898..15251)
                     /locus_tag="PK288_04320"
     CDS             complement(14898..15251)
                     /locus_tag="PK288_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18175.1"
                     /translation="MNSLREIESKLLLYFSIVRDGCRNYVSECEYTPYNCGYSEGSER
                     PVNTYELCMFVTGVVKCPGEIEKHYRERHNKRVCRTILRAIERLEKKGFIIKDNRSAG
                     VISQTDKWHMTYRMV"
     gene            15622..16677
                     /locus_tag="PK288_04325"
     CDS             15622..16677
                     /locus_tag="PK288_04325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPM18176.1"
                     /translation="MKNINRRKFLGNGMAAAAMVTVGKSLPSEKVDIMVPFRKNHSEP
                     FNPVTFNAMPTRSFGKTGYKVGILSLGGQATIEIKGMEEESEKIINRAIDLGVNYIDT
                     AASYGQGVSQLNIGRVMKTRRNEVWLSTKTHDRTYDGSMRLLEESLKNLQTDHINLWQ
                     LHNVQRQDQVDQIFAPNGAIKALEKAKSEGVVKYVGITGHYEPLVLLEAIKRYPFDAI
                     LLAVNAADVHYLSFKNYLLPAAQEKGIAIIGMKVTTRSRLLSSWTPPPLEQQHEQLRT
                     SKPGTISIREALTYNMSLPVSTTIIGVDNVAQIEENVKIASEFSPLSQQQMEEIEFKT
                     LPVVRQALFFRRWELGA"
     gene            16721..17692
                     /locus_tag="PK288_04330"
     CDS             16721..17692
                     /locus_tag="PK288_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HPM18177.1"
                     /translation="MKNIIRYDGIICLVLLMTGVSCNKGELPVLTTSDITKITTTSAV
                     TGGNIISDGGSTITARGVCYNTSGNPTIANTRTLDGTGSGSFTSDLSLLVPGTYYTVR
                     AYATNSAGTAYGNEVSFVTSKIITGTVDDIEGNSYRTVTIGNQVWMAENLKTTRFNDG
                     TEIPLVSGNTEWKSLNTPGYCWYNNDEAANKPVYGALYNWYAVASGKLCPAGWRIPSN
                     DQWKTLTTFLGGEYLAGDKLKEEGSEHWKHFNDKSTNESGFTALPGGSRIDGSYVLIG
                     EMGAWWASTPFSEEEAYLRELDDFVTEVLSGYLNKKYGFSVRCIKNN"
     gene            complement(17809..18825)
                     /locus_tag="PK288_04335"
     CDS             complement(17809..18825)
                     /locus_tag="PK288_04335"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPM18178.1"
                     /translation="MMTDREEGRIISRRDFSRKGLTAAAALATVGLGGYGLLSTGKGR
                     SLKNYYRMGHCAPSVMQTLVETGRTGHSGLVLYTGAMSGGIAGPSMECGALTAPLIYM
                     GFEEKKPAGLSEKLELISRGQDYIKRFEEFNGTCICGNIRQGGMPACRRAVSNFYGLY
                     SASVKKPYPVPDVARQSYAILLSEFDSRNFHCAGNVLKNLDGKFRPAEELYDASWLLV
                     GGLAMLNRTCGALAAGVMALSSVTAEIEKNFFRVARMNRLFKANNNKAMDEDMNNFNR
                     SIHFSENLGIWFRKEFGSTTCLDICGANFSVAGDAENYLSGNYIGLCSDIAKRVAEKV
                     NSMI"
     gene            complement(19204..19719)
                     /locus_tag="PK288_04340"
     CDS             complement(19204..19719)
                     /locus_tag="PK288_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18179.1"
                     /translation="MNKENSPQPKPDLKQAILHMTAEGREDFLLYLKNIGLLSEPNIL
                     VLSPGQHYYYDESDLRSLKILVNMKKLNLVRHLDRFLHNLYRILPPEASFIGCFSDSA
                     TERAYNVKHGYSSGKLLSSIRNLFGYITEQKMSGIKVSEILKTHGFKIIDMSEINGVT
                     FFWSRNVRRSN"
     gene            20572..23913
                     /locus_tag="PK288_04345"
     CDS             20572..23913
                     /locus_tag="PK288_04345"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HPM18180.1"
                     /translation="MKKNHSITQMRYAMIILMILFSCLGAYPQVKLTQEDWVIPTWAV
                     LPADKNPMFFKKESYQGASKVIYPYAMNDMISDVKTDKAWKTITLENEYIRLAVTPEI
                     GGKIYYAADKTNNYNFIYKNDVVRPSNIGMLGAWVSGGIEWCVLHHHRASTFLPVDWS
                     ASENSDGSKTVWVGETEPRHRMRWTIGITVFPGRSYYQAEVKITNPTPYTHSFLYWAN
                     VAAHTNRDYQVIFPPSVQIATFHAKNSFTHWPFSTEIYSGQDFTRGVDISWWKNAENS
                     NSFFAHDLKEDFMGGYDHGRQAGTVHIGDHNIVKGAKLWEWGSGPRGQATEARLTDKS
                     GPYVEIMVGAFSDNQPDYSWIRPYEVKVFKQYWYPVKDIQGFKNANLNGAVNLEAREK
                     NQVFLGYYSTRKVAKANIVLKQGDKTIFETTTEISPEKAFTRTIKPGVPFIMEDLYTQ
                     MSDQETGEVLVSYKPVAIKREDKLPEIVKRPDEPKDIPTIEELYLAGSRIQQFYNPTL
                     DPMDYYTEALKRDPGDIRTNTAVGNYYLKNGDYTRARACFSRAIKRLTKDYTRPSDCE
                     PLYLQGLTLKAMGLYDEAVDTLYRATWDHAWHSAAYLELARISSMRGDFLKALNETDE
                     SLSTNSRNNPAIELKAALLRKTGDLKASSELLETIVKQDPLDFRAANEYYLVLKASGD
                     RQKADAVLADLAGKMRDFDQNYLELAISYLNDGFLQEAEDVLKRFRGSNPMVNYYLGY
                     ILDKNGNRPEALKQFRSGSALPVDYVFPFRLETVNVLRLVSSCLPDDARPWYYLGNLL
                     YDKQSATAVECWEKAVSLDPQLAIAWRNLGWGYYYAMDNGERAIEAYEKAIALEKNEP
                     VYYTELDALYEMSNTPVEKRLRLFDGSNETVRKRDDSFIRQIVVLTLAGQPEKAVEYL
                     AGRNFSYREGSSRVREVLIDAHLMMGRKHMEERNFKKALDEFMLARVPDEEGGSARAG
                     AREVQVDYHIGLAYEALGNKSKARSYYTMAAKPEIRDGNYIRYYQALSQAKLGNKTKA
                     GEIFNSMMDAGEKQISSSGSDTDFFAKFGEREARNVILSEAYLLKGLACKGLGNIEPA
                     KENLEKALELSAGNLYAKVELMDLK"
     gene            complement(23946..>25271)
                     /locus_tag="PK288_04350"
     CDS             complement(23946..>25271)
                     /locus_tag="PK288_04350"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPM18181.1"
                     /translation="YLIRLLEEQPVGSTGSVVFDFGGKPRVAKRPPWEGGITWAKDGG
                     GRPFIASSCQGDGASLWWPCKDHLYDEPDSMLISVTVPRELTAVSNGRLRSKTQNRNM
                     TTTFHWFVANPINNYGVNINIARYSHFSEFYQGEKGRLDCDYYVLRENLEKAKSQFKQ
                     VARMLEAFEHWFGPYPFYEDGYKLVEVPYLGMEHQSSVTYGNRYGNGYLGEDLSGTGW
                     GHRFDFIIIHESGHEWFGNSITNEDAADMWIHESFTNYAESLYLEYYYGKEAASEYIL
                     GCRRRILNKRTITGIYNVNFTGSGDMYYKGGSMLHTLRQVIDDDEKWRDILRGLNREF
                     YHQVVKGSQVENYIGSRSGLDLKPFFDQYLRDIRIPVLEYFIDGSKLNFRWTNCVPGF
                     NMPVRIFVSGRAMKINPTEDYNELDLGTFNAEIVIDPRYYAASMNITGK"
BASE COUNT         7194 a         6005 c         5548 g         6524 t
ORIGIN      
        1 tcacgcgtgg gcggggtggt caaaagcaag gattattcaa gctttgatgt agggattgcc
       61 gtcaacagca tgatccttca ggctgctgca gaaggcattg gttcatgcat cattggctgg
      121 ttcgatgaaa aggaggttaa aaggatcctt gggatccctt catccagaag ggttgatctg
      181 atgatcaccc tcgggtatcc ggaaggagaa cgcaggaaca aaaagcgcaa accgggggat
      241 gtgaccgtat catataacag atattgatgc tgaagccggc ccggtaacca gccggaatgc
      301 cggtctgtta ttattttaac agttccagcg cttccctcat ccgcctgacg gcctcagcca
      361 gtaattcatc tgaattagcg tacgagattc ttatacatga gggagcaccg aatgcatcac
      421 ctcccaccac ggcaatacgg gctttatcta gcaggaaaag tgcaagatcg tcagaagtgt
      481 taattttaac gctgccatcc gacttaccca gataatagga aatgtcgggc ataacataaa
      541 atgctccctg gggcacgctc accttcagcc ccggtatctc tttcagcatt ccgcagataa
      601 gatcccgcct ccggtgaaac gcttccttca tcttccggcg tgaatcatcc tgaccttcaa
      661 gtgctgccag cgcggcccgc tgtgcaattg aacatactcc cgaagtaatc tgtccctgca
      721 gtttattgca tgcttttgcg atccacagcg gagcacccag gtatccgatc ctccagcctg
      781 tcatggcata accttttgaa accccgttca ccgtgatcac cctgtcgtaa ataaagtcaa
      841 aagatgccat gctgacatgc tccccttcaa aaatgatatg ctcatagatc tcatccgaca
      901 tcacaaaaag gccctcatgc ctttcaacga ccctggctat ggcctccatc tcattccctg
      961 tataaaccat tccggttgga ttggatgggg aattgaataa caaaagacgg gtcttttcag
     1021 ttacagcttt ctcaatctga cccggactaa ccttgaaatc attttcaatc tctgcttcca
     1081 caataaccgg cttgccgccg gcaaggacga cctggtcata atagcttacc cagtaaggtg
     1141 caggtattat cacctcgtca cccgggttga taatgcacag cattacattg gccagggagt
     1201 gcttccctcc ggctgaaaca attatctgat caggcgtata ttcaagtccg ttttcttccc
     1261 ggaatttcct tgatatggct tttctcagat catcgtatcc gggaaccggc ggatattttg
     1321 tgtaattgtc atctatcgac ttttttgcag cttctttgat ataatcagga gtattgaaat
     1381 cgggttctcc cagactgagg cttattacat cgagtccctt tgccttgagc tcacggctct
     1441 tctgtgccat tgccagagtt tgcgaatctg aaagcgccct gatacggtca gacaggtatt
     1501 ccataggttt gattttttta atgttttttc atggacggtg tcaaatgtat aaagaaaatc
     1561 gaaaatgcaa aatatttgtc cggagaagaa tccgatgccc gaaagaggtt aacaggaccc
     1621 tccggctgcc gggaactaac tgatctcccg gtcatgcagg tttgcgtgat gcataactac
     1681 acggggaaat tcggggttaa cggcaagatg tttttacatc gcgatgatat ataataattc
     1741 tcctggtaac atataattgc ggttcaggtt tttaaacctg tcaggcagga atgggtatat
     1801 taccggaaaa aattttacgt ttgtatgtta aaatgaatgt tatggagacc ctttacgaga
     1861 ttctgaaaat tgtattacca gccgccattg tcctgctgac tgcctgggtt attttaagga
     1921 acatgatccg gaacgatcag aacaagagga ggcatgaact gattctgcag ggtgcccgta
     1981 ctgttacacc aatacggctt caggcctacg agagaattgt gttgttcctg gaaaggatct
     2041 caatggaatc gctgcttatc cgtatcagta ctcctgacat gacgacaggg cagcttcaga
     2101 cggccctgct gtccgctatc cgaaatgaat ttgaacacaa tttgtcacaa cagatctata
     2161 tgagcccgca ggcatgggaa attgtgaaaa ccgctaggtc gaacacaata aaaatcatca
     2221 acagcgaagc tgaaaaaatt aagcccaccg ccccggcaat cgaacttagc aaaagactcc
     2281 ttgagaaggt aatggaactt gacaaggagc caaccagggc cgcaatcgaa tacatcaaga
     2341 acgaagtgtc ggaaattatc tgatctccat gaaggcgcac cgttcagccg gtctcggcgg
     2401 tacctctcca acaggtaatc gcaaaccccg gactcgctcg ttctgcacct tcattctaaa
     2461 gaagctcaac gcttctcctg acaaatgtgc caagctcctc tcccctgagc atattgttgg
     2521 ccagcagtgc caggtcaatc agctgcttta caaccttgtt ttctgttccg taggagccta
     2581 atatatcttt tcttgcattc tcgcaggttt ccagttcctt acgcagcttt tccagatcat
     2641 ccttttcaaa ctggcttatt tcctctgcct ttttcttttt atgctcattc tccatgaacg
     2701 agatctcatc tttaagcctg ttcctttcag aattattctc cgtaagcctt tctcctgcct
     2761 tttcctgcag atcatccgca atcctgagga tcagcggatg gttaagatta accacaatat
     2821 tgtaactgtc gggcaattca ccgtaaaagt tcatacctcc gccaatctgt gacatctctt
     2881 tcatcctccg catgaactca gactgggtta tgatgacggg agcgtcattt tcatcaagag
     2941 tttctgtcac cacgttaaac acttcctttg atgtcatttt caggttaaac actcccctga
     3001 tgtcatcctg ccggtccttt gtcagttttg attctttctg atcctccttc tgaataagct
     3061 tatcaataac atccgaatcc actcttacga acctggagtc cttgaacttt gtctcaaggt
     3121 gattgatcag gtgcacatcg agctgtccat cgagatgaag aatatcatat cccttattcc
     3181 tggccttttc aatataggtg tactgatcgg tcttgtttgt cgaatataga taaacaagcg
     3241 tcttgttcct gtcagtctgg ttttccttaa tcagcttctc gtactcgtca aaagtgaaaa
     3301 atttatcttc cgtattttta aaaagggcaa atttcgaagc tttctcataa aatttctctt
     3361 ctgtgatcat cccatagtcg ataaacagct tgagactgtc ccatttcttt tcaaattctt
     3421 cgcgatcttt cctgaatatc tcgttcagtc tgtctgccac tttttttgta atgtgagccg
     3481 aaatcttttt tacgtttgtg tcgctttgca ggtagctcct cgaaacattc agggggatat
     3541 ccggtgaatc aagaactccg tgaagcagtg tgaggaactc gggtactatc ccttcaaccg
     3601 agtcggtcac aaaaacctgg ttacaataca gctgtatctt gttcttctgg atctcgatat
     3661 tgctttttat ttttggaaaa taaagaatgc ccgtcagctt gaaaggatag tccacattca
     3721 ggtgtatatt gaaaagaggc tcttctccca tcggatacag ttccctgtag aaattcctgt
     3781 aatcttcatc cttaaggtcg gccggcttaa gggtccaggc cgggcgggta ttgttgataa
     3841 ttttatcctt ttcagtatca acatattttc catccttcca ttccttttcc ttgccgaatg
     3901 caatttcaac cggaaggaac ttgcagtatt ttttcaggag ctgttcgatc ctgtcttctt
     3961 caaggaactc ctttgaatcc ttgtcaatat aaagaatcac atctgttccc cggttttcct
     4021 tttctgtttc cgcaatctga tattcaggac tcccgtcgca gacccatttt acagcctgtg
     4081 ccggttcatt ccatgatctg gagatgactt caaccttgtc ggaaaccatg aatgaggaat
     4141 agaatcccag accaaaatga ccgataatgg aattggcctg atccttatac ttatccagaa
     4201 aatcatttgc gctggagaag gcaatctgat tcaggtattt gtctatttca tccgctgtca
     4261 tccccacacc gctgtcagat accctgatgg tttttttctt cttatcaacc gacacccgga
     4321 tggttaaatc cccgacttcc cctttaaaat ctccggcaga tgcgattgtt ttgagctttt
     4381 gtgttgcatc cactgcattt gatatcagtt ccctcagaaa aatctcatga tcagaataca
     4441 ggaacttctt gattatcgga aatatgttct cggtagttac tccaattttt cctgtttgca
     4501 tacggattta tttatttatt agacattatt tacgccgcct caatttcaaa aggcgtgccg
     4561 ctgtgtgaaa actgacaatt agtcacctat tccttgttcc tggcgtcaac atccagcctg
     4621 agatgccagg ttcccttgtc aaaacgatat gcatcatagg aatagtcagg gccgtagaat
     4681 tccctgttgt tcaggtattc aggggatata ggtacaaggt ggtcgaatac tatcattttg
     4741 tcagacatga atctgagtga gatcactgca gtaaagctgt actcaagcac ttcccggtat
     4801 ttaacttcgg ttccggtgtc gaaccatttt tttccgaaaa ccggcctgtc cgcgtcatca
     4861 aaactgagaa cgtcaattac tttccttgtt attgcagggt tgccataatc aataccaaga
     4921 atcatccaga aaacttcgtt tccttttctc accggtctca tatcataata aagagcccca
     4981 taccagtcct cctgaaagta agaagtatcg gtcctgatac tgtcagggtt gtaatttgcg
     5041 ctgagcctga ggatcctgtt gccctccttt ctcctgttaa tgaaataaca gtagtacctg
     5101 ccaggatttt ctttcaaagg aagattccag gtgatgatct tcatccttga atccttcgac
     5161 gttatttgtc caagatattt cagatttgaa aattcatgac tgaaaatgga atctgaataa
     5221 acgtagctgt cgattatcaa tcgtatcgag tcgctggcca gtattttctg ctggtctgga
     5281 atccctgaag caattctgcc aaaaagcctg gaaagttcat cttccggatt gttttcacgg
     5341 acaacagcaa ggtttgaaat aactgctgag aagaatataa gaattgcggc gtaaaatttc
     5401 attcctttct ggtatttgcc attgtatcat ccagaagatc gagtatctgc cggcatgcaa
     5461 tggatatttc ttccccggag atgatcagcg ggggtgctat cctgaaggct gagtcgcaaa
     5521 aaagaaaata atcgagaatc agtccgaacg agggagcatt tgccactgca taacttacat
     5581 gacggggtga tgcaagctga accgcaagca tgagcccctc gcctcttatt tcagtaatca
     5641 ggggatggac gaggagtttt ctgaacaggt ctgacttgaa ggcacatgat ccgacaagct
     5701 tttgcccgat tattacatcg agtgaagcca gccctgcagc gcagcataca ggatggccgc
     5761 cgaaagtggt aatatgtgac agcgccggat cagatgcaag ggatgacata ttttcccttg
     5821 atgaaataaa tgccccaagc ggcattccgc ctccaagggc ctttgccagc accagtatat
     5881 cgggaaccac accatatctc tccagggcaa acatggtgcc tgtcctgcca aaacctgtct
     5941 gaatctcatc aaaaatcagc aatgcgcccg actcatcgca tttctgcctt agttttttca
     6001 ggaaatcgtt tacaggaaca ataattccgg cctcaccctg aattggctca acaatgacgc
     6061 aggcagtatg gtcgtcgata atctcaagtg actccgcatt attgaaatca gcaatgtaaa
     6121 tatcaggaac aagcggccgg aaaggattcc tgaacttctc gtttccctga accgacaatg
     6181 ctccaagagt gcttccatgg tatgaattcc tgaaagagat tattttgctt ttccgggtca
     6241 gtttttttgc cagtttgatg cttccttcaa cagcttcact cccagaattg acaaaatagc
     6301 agcactcaag cggttcgggc agcaatgagg ccagctttgc agcatatttc acctgcggac
     6361 tttgaataaa ttcaccatag accatcaggt gcatatactt gtcggcctgc tcttttacgg
     6421 catctaccac ttccggattg caatgccccg tgttgctgac tgaaactccc gaaataagat
     6481 cgatataccg cttaccgtca ggtgcataca aataaatacc tgatgctttc tcaatctcca
     6541 gaagcatcgg catttccgag gtcttaccca aaaaatcaag aaaaagccgg cggtttgaaa
     6601 gcatgttgca ggaaggcact atttcgccat cacattaatg tcatttagca gggaatcctt
     6661 gaatgatttc ccgaccggta tattcttcac agtgttgccg attgtaatat ccagcgagtt
     6721 ttccttaatg gtctttatca tgcttttatt tatgatgaac gatctgtgca cacgcaggaa
     6781 aacactggaa ggcaactggc tttcaattgc cttcattgta aaatgaattg tatacttgtc
     6841 ggtcttggta ttgagagtca catagttttc aagggcttca acaaaaacga tatccttgag
     6901 tttcagtttt accagggtgg agcccttttt aataaatatt tcctcatcct ctcccggact
     6961 gtattccttg ggtgaaaaat accttatcgt cttgtcaatg gctttgcaga acctcgaata
     7021 ggtaactggt ttcaaaagaa aatcaacaac gttgaagtcg aaggccctga gggcaaactc
     7081 actttcacct gagacgatta taatgtttgg gggatggtcg aggctgccga taaaatcgaa
     7141 tccgtccatc tccggcattt taatgtcaag cagaataagg tcaatatcct tgtgtttgat
     7201 tatttcattt ctggcagaga tggcatcata aaatacaccg atcaggttaa gggaagagta
     7261 cttggtgaca tatccctcca aaactttgca gctgagtttg tcgtcatcaa caataatgca
     7321 gttcattatt ccattttttg aatcggaatt aactttttca aaaatagtaa aattttttaa
     7381 atcaaattca agattcagaa ttaattccct tcagtcatgc ctggttctga accaaaaaat
     7441 atttccggaa ttagtgctat aacaacctgt gtataacaag gcggttcatc cggagattgc
     7501 atgactgaca ttggttcaaa tgtgatcacg aggcccggca tcataaaatg tcagcctgca
     7561 ttgccagggt tatcggaaga gtatcggaaa ttccaaaaaa agaggggtgt atcgcgatac
     7621 acccctcagg caatctctaa gtgtaaaaat taaagacctt tgttgagtgc cggagctgct
     7681 ttgaatttta cgactttctt tgcaggaaca tttaatttag cgccggtacg gggattgcga
     7741 accactctcg cgctcctttt gttaacggta aaggtgccca taccgggaag ctggatcctc
     7801 tcgcctttct tcatagcttt tccaaaagct gcgacgaaag cgttcagagc tttgcctgag
     7861 tcttttatcg agaggccggc atcattagca attgcattta ctaattcttt ttttgtcatg
     7921 acgaatgatt taaaggttag accaaaggtt ctaattgatg caaatatatt ccaatttata
     7981 tgatgtgcaa ataaattgat cccattttcc gcaaataatg gtggttttag aacaaaagtt
     8041 ctggtttttt ggcaaaccag ggtatttcat taatgcctat caagatcctc attctgtggc
     8101 actttgctct ttttttcgca ttcatgattg ttaaaatcct gttaataaat agtttaaaac
     8161 attgaatgac tgacgattga tatattttaa accataagcc tgataagtta aaaatgtatt
     8221 atatttgctg cggataccca tgggcaggcc gggaatccca tacctgaccc gtgcctgtaa
     8281 tcatcccgga tcattaaaaa taacctttcg gggggaacag gcgggatatg aatggagaaa
     8341 tggcagagcg gtcgaatgcg gcggtcttga aaaccgttgt tccgctaccg cggaaccggg
     8401 ggttcgaatc cctctttctc cgccaaaatc atcatctcag acccgcagct tcagctgcgg
     8461 gtttttgttt cacacggatt acgagccatg cccggcatga gcgaggaagt cgtgtgaaac
     8521 aaaaaacaag cccgcagggc ggctgagatg gtgattttga agcatccccc aagggatcac
     8581 cgacgaacga agtgaggagg taatcccgcg gaaccgggag tatctgccct gcagcccggc
     8641 tccttcgcta aaaacaccca ccgggtgttt ttttacgctc ggtcctctct ttctccgcca
     8701 aaatcatcat ctcagacccg cagcttcagc tgcgggtttt tgtttcacac ggattacgag
     8761 ccatgcccgg catgagcgag gaagtcgtgt gaaacaaaaa acaagcccgc agggcggctg
     8821 agatggtgat tttgaagcat cccccaaggg atcaccgacg aacgaagtga ggaggtaatc
     8881 ccgcggaacc gggagtatct gccctgcagc ccggctcctt ccttcggccg ccgtaagctt
     8941 gccaaaggcg gcgcgtcaac catgtatata atctccggcc tttacgcccg ccacaaccca
     9001 atctgcagtt ggtttcgcca tgcttcaggc cgggcagtac aatttactga ccattgccat
     9061 ggattacggc atgtcaggcc ggcttccggc agttgtcgac actccattta ccggcaccgt
     9121 atgaagcaat gaaaaggaac aggaaactga aaagcaccgc cagctctcca tgattattga
     9181 taggcagcaa tccggccgag gaatgtccta tgaaatatgc cgcggccatc tccccactgg
     9241 cgataaacgc cgttattctt gtctgccatc ctatcaggat cagcagcccg ccaactgcct
     9301 caataacagc agcaatgatc atcatgacat tgtcaggtgg catactgccg ctcagaaatc
     9361 cgccgatctt ctgaatacca tgcggtataa acagcagtcc tgtgactatt ctcaggaggg
     9421 caaaaatctc attctggtat ctttcaagaa atttcataac aaacaatact taatgatttg
     9481 ataattctgt tttgttcctg accgattaaa caccgttaca tcatttatgt tcaaaccgaa
     9541 tgcaaccatc tgtgccttac aggctacatg gtatctgacc cggcaaagtt cacgaagctg
     9601 cacatgggat acccggttta atcagtggct atatgaatcc ggattcattc tgacataaat
     9661 cactcctgcc tgctgtcaga tcctttccat cactttctta aagggcgcca ggagtgtgtt
     9721 ccatggaatc tgcgaaagaa ccatcagcac aagtacggca ggcgaaaaat atgatacggc
     9781 aagaacaatg gccagcccgt tattcatgta tgctgaccct atagccaccg caatcctgct
     9841 ttccctgttt tcacggggga ttatcatgta accaatgata tgcagcagta tgaataacag
     9901 gtataatgct gccagcttcc ataccagccc agccggattc ccgaggatga cctccctctg
     9961 tgatgaaatg gttacaaaca caaacgaaaa aataatcagc acattgaagg aggttaggag
    10021 atcatgcaaa ccagccacga caccgggaag gagttttttt gtcaactgtg acaggatcat
    10081 cgggacaaaa accaggataa ctatatccct cagcatcaga aattcgtcaa tgtcgaccag
    10141 ccttatgtgc accagcctga acagcaatgg aacagtaaaa ggtgctatga gctgggtcag
    10201 tatggcaagg ctcatggaaa gcggaatatt accctttgcg atatccgtca gtaccggcga
    10261 tgaaacaccg gcaggcatgg ctgtgaggag aaggatgcca acggcaaact catggtcaaa
    10321 tatccccgac aggaaataaa atcccagcgg gatgacaatc atgtaaacaa aggtgatcca
    10381 cgacatcagc cggagatttt tcatgttctc aaggatttcg atggcatcta tcttcatgaa
    10441 agccacgaaa agcatcattg ccaatagtat cttctgcaca accggcgatg tgttcacttc
    10501 aaagggaggc cagaggccga gaatgatacc ggcaatcata acaacccaga aatgcgtctc
    10561 aactagcctt atgatcttca tgcatctctc cgttataccc ggcaggccgg aaaaatctgc
    10621 cggcaaagat caggaatttt gtttttcggt aaacaatcat gcctgaattt ttgtataacg
    10681 cttgctttaa tgattgttaa atgtatttca tactggtttt atttctgcta aatcaggatt
    10741 aaattcagcc ccgttgatgc ggaagccgga tttattccgc tctcagtaaa agtccattta
    10801 caacatgaaa ataatagttc ttgacggata tgctttaaat ccgggcgatc tgagctggga
    10861 aaagcttcac ggcctgggtg atgtaaaaat ttatgacagg accccggctg agcttgtcct
    10921 gaaacgggcg gaaggcgcgg agatactgct taccaataaa acgatactgg aggcaggaac
    10981 actgcagttg cttccgaaac tcaggtatgt cggggtgctg gcaacggggt acaacgtcat
    11041 tgatacggct gaagcccgca ggcttgggat taccgtgacc aatataccgg cgtacagcac
    11101 cgcctcggtg gcccagcata ctttcgcact gatactggaa ttgtatcttc acatacagcg
    11161 ttacagcgat tctgttcgcg caggcaaatg gtcaaagtca aaagatttca cataccgcga
    11221 cctgcccctg agggaacttg ccggcaaaac tctcggcatc atcggttttg gaaacatagg
    11281 tagaaaggtt tgcgaaatag ccatggcatt tggcatgaaa gtggttgcat attccccttc
    11341 gaagagcaat gcaccgcaat atgacggatt tgaatgggat gaacccgacg gccttctttg
    11401 ccgttctgac attgtcaccc tccactgtcc gctaacccct gaaaccgcag gaatgatcag
    11461 caaaaccaaa ctgaaactga tgaaaaagag tgcattcctg ataaatacat cacgtggacc
    11521 tctgatcgtt gaggaagatc tggccgatgc tctcaataca ggcatcattg ccggtgccgg
    11581 gctggatgtt ctggccctgg aaccgccccc gtcagacaac cccctgctga aggctaaaaa
    11641 ctgcatcata acaccccata ttgcctgggc aaccattgaa gccagaacca gactaatgga
    11701 aatcgcggta gagaatatcc gggctttcat tgccggatca cctgaaaatg tagtcaacag
    11761 gtaatgctgg acttctttcc acacgattgt ttcgacactg ggtggcttac ttcagctatg
    11821 cttttacggg tagtgggttc ttttcctata ataaatcaat ttcattccta atcaggcttc
    11881 tcatatcaaa gccactattg ccggtttcat ttctgaggct gcctgaaaag ggtctgatga
    11941 aaaaataccg aagctgtttt acttcactcc ccgaaccgga tatgtgcaaa taattacatt
    12001 cccgcaggat cgttaattga tcaggggtca aggaaagcgc tccctccggg ttaccccgga
    12061 acttcaaaag tcgtgactca gcatccgaag taaccattgg aaagtgtttc attaaagtgt
    12121 gcccggcagt ataacgattc ctgatataca tcccggatcg gggatctcca tgacgaaggc
    12181 ggggaaaaat ggattataaa aacggtattc gcattagatg ccgcacgccg tgttttttag
    12241 aataatccgg ctgaattgct tatttaacct ggctttaacg ctgtaataaa cttttttacc
    12301 cgacgtttta tatatgataa aaggacttaa ggattagatc caaatggtta acgaaaaaac
    12361 aaacccgaat gaacgcagtc tcgttcaatc acctgtggct tccagtccgc gggatcaggc
    12421 aggcatcagg catttaaaga ataaatcata taggtatgaa acccacatga ttcaaaatca
    12481 taaaaaaaca tgaaaacata attcatgtgg gttccatgtg aaccacttaa tcttcagtag
    12541 tatataaatt tattttcaca tgaaaaaacc tggaaccttc cttataacat cagccattgt
    12601 gatattgctg tttggtcaag gtatcatacc ggtcaatgaa cttatggtcc gggctgttac
    12661 tcaaacgaca gcggcgacag ctgcacaatc caaacctgca ccggctcaga aacagacagc
    12721 accggcacag aagcagacag caccggccca gaagcaggca acaccagccc agaaacaggc
    12781 aacaccggtc cagaaacaaa caacaccggc acagaagcag gcagcaccgg cccagaaaca
    12841 gacaacacca gctcagaagc aggcaccacc ggcacaaaag caaacagcac cggctcagaa
    12901 gcaggcacca ccggctcaga agcaaacaac accggcacag aaacagacaa caccggctca
    12961 gaagcagaca ccaccggctc agaagcaaac aacaccggca cagaaacagg caacaccggc
    13021 ccagaagcag gctgctccaa cccagaaaaa atcccccgct accgaaccag gaacaaaaac
    13081 cgccatcaaa actccggaaa ctggtatcgt caggatagga tcccaggtat gggctgcaac
    13141 gaacctcaac gtcgtaactt tcaggaacgg tgacaccatt ccggaggcca ggaccaatga
    13201 ggaatgggta gcggccggca aatcagaaaa gcccgcatgg tgctattata acaacgaccc
    13261 caaaaacgga tccaaatacg ggaaattgta taactggttc gccataaacg atccgagggg
    13321 tcttgcgcct gaaggctggt cattgcccga tgcatctgac tgggctcagc ttgcaggcta
    13381 ccttggcgga gccggcactg cgggaaacaa gctgaaaagc agcagtggat gggctgacgg
    13441 ttatataggc tcaaacgaat cgggattcag cggattgccg ggtggatacc gggtagaaaa
    13501 cggtaatttc cataatcttg ggagtattgc aacctggtgg agcatcacgg aaggcaagcc
    13561 ggggacggcg atcgaccatt acatcggaca gcgtggatcg ctggaaagaa gccacaatcc
    13621 gaaacaggtc ggagaatcgg taagatgtat caggaaacag taacagaatc acaggatatg
    13681 gcataaggct ccttcagtga aactcacacg cggtcatctc attaatatta tttaatgatt
    13741 aattttgcca tgaaacccaa aggtatgaaa aagcctcttc ttaaatattc agagccatgg
    13801 taatagggat agacattgga agtacaacta ccaaagtagc ggcggtgaat tcagggaaaa
    13861 agggtatcat tatggtcagg acaaaggctt tcgacgctat tacctcggca actggagcat
    13921 tcggtaaact gcttgtagag aataatttaa acctggccaa tatcgagaaa ataaacatta
    13981 ccggtgtggg agcctccagg atcccgggga acctgttcgg tattgccact accaggatag
    14041 atgagatggt tgccatcgga actggcggaa aataccttgc aaaaaaggac cggatcatca
    14101 tttccaatat cggaaccggc acggcaatca tccaggttga cggctccgcg atcactcacc
    14161 tgggaggcac cggagtgggt ggcgggacta taaccggact ggcccggcag ctgcttaata
    14221 tctccgattt tgataccata atgaactatg ccgcaaaagg caatatcaac aatatcgatc
    14281 ttcagattgg cgacattgct gaaggcaata ttggttttct caacagggaa tacacagctt
    14341 cgaacttcgg aaaaatgctc gacactgcta ccagggaaga tattgctgcc ggtatcctga
    14401 accttacata ccaggtaatc ggtatggttt cggtctttgc agccaggagc aagaacaacg
    14461 acaccgttgt ggtaaccgga aacggcagca acaatatact cggccaaaaa atacttgcat
    14521 ccatctcaag tatgtataac attcaatttg agttccctgc aaatgccgaa tttgctacgg
    14581 ccataggagc ggcgcaaagc tgaaaacaaa cggtaacagt gtgtcggatt tttcctgatc
    14641 gttgatctta tccggagatt cagtgattaa ccatttttaa gcccggataa atgtgcaaat
    14701 aatgcagaaa gtgccgggac agaatcagaa attcagtatt tttgcggatc cgggacgtta
    14761 gctcagccgg ttcagagcgc ttgcttcaca cgcaagaggt cgacagttcg aatctgtcac
    14821 gccccacgac cgtaacctga catttcctga actccatcat tgcggggctg tgtttatcag
    14881 ttcctgtcgg cctgaattca taccatcctg tatgtcatat gccatttatc ggtctgactt
    14941 attacaccgg ctgatctgtt gtcttttatt ataaaccctt tcttctcaag tctttcaatt
    15001 gctctcaata tggtcctgca gactcgcttg ttatgtcttt ccctgtaatg tttttcaatt
    15061 tctcccggac attttaccac tccggtcaca aacatgcata attcgtaggt gttaaccggt
    15121 ctttcgcttc cctcgctgta accgcaatta tagggtgtat actcacattc gctcacataa
    15181 ttcctgcaac cgtcccgaac tatgctgaaa tacaggagca atttgctttc tatttccctg
    15241 agactgttca ttgctttttt aaacagaact gttcattccg ttaaataaat gcttcctttg
    15301 cccggtacca aaccgccctg ccgaagtatt aaagtcccct gaatctgaga tttaaagtta
    15361 cgacaaaatc cgggtctgag caactaaact gtcacaataa attctgagtt tttcatgctt
    15421 ccggctttgt ttcaggaccg gttcagagta ttatccaaac tgttgttaaa catttaacag
    15481 ttcatcacgg cacatgaaaa tccggtttcc cggaaattat ctgccctgat tatcttaacc
    15541 ctttttacag aaatacctta aaaaaataag taaatttgag cttgaaataa tatctgtaat
    15601 taacatacaa aactgacacc gatgaaaaat ataaaccgcc ggaagtttct gggaaacggg
    15661 atggctgcag cagccatggt gactgtaggg aaatccctgc cgtcagaaaa agttgatata
    15721 atggtccctt tcagaaaaaa tcattcggag cctttcaatc ctgtaacatt caatgcaatg
    15781 cctaccaggt catttggaaa aacaggatac aaggtgggga ttttgagtct tggagggcag
    15841 gcaaccattg aaatcaaagg catggaagaa gagtcggaaa agatcatcaa cagggccatt
    15901 gatctgggtg ttaattatat tgatacagcg gcttcttacg gtcagggagt gagtcagcta
    15961 aatattggca gggtaatgaa aacccgcagg aatgaagtgt ggctctcaac caaaacccac
    16021 gacaggacct acgacggatc aatgcgcctc ctggaagaga gcctgaaaaa tcttcagact
    16081 gaccacatca atctctggca gctgcataac gtccagcggc aggaccaggt cgaccagatc
    16141 tttgcaccca acggagcgat aaaagcactg gaaaaagcca aatcagaagg tgttgtaaag
    16201 tatgttggca ttacaggtca ctacgaacca ctggtgctgc ttgaggcaat aaaacgctat
    16261 ccgttcgatg caatccttct tgctgtcaat gctgccgatg tccattacct gagttttaaa
    16321 aactaccttc tgcccgcagc acaggaaaaa ggcattgcaa ttatcggaat gaaagttaca
    16381 accaggagcc gcttactgtc ttcatggact cctccacccc tcgaacagca gcatgaacag
    16441 ctccgtacct cgaaaccggg aacaatatcc atcagggaag cactgacgta taatatgagc
    16501 ctgccggtca gtacaacaat aatcggagtt gacaatgtag cccagataga ggagaacgta
    16561 aaaatcgctt ccgaattctc ccccctgagc cagcagcaaa tggaagagat cgaattcaaa
    16621 accctgcctg tggtacgaca ggccctcttc ttcaggagat gggaattggg agcctgatgg
    16681 agacacgttt gtttctatgt ttccatttaa tcagcatgtt atgaaaaaca tcatcagata
    16741 tgacggcatt atctgtcttg tactgttaat gaccggtgtt tcctgcaaca aaggtgaatt
    16801 acctgtattg accacttccg atataaccaa aataaccaca acttcagctg taaccggggg
    16861 aaacatcatc agcgacggcg gaagtacgat cactgccagg ggagtctgtt acaacacatc
    16921 ggggaatccg acaatcgcca ataccagaac ccttgacgga accggatcag gttcattcac
    16981 aagcgatctg tcactgcttg ttcccggcac ttattacacc gtaagggcat acgccaccaa
    17041 cagtgcaggc acagcctacg ggaacgaagt cagttttgta acatcaaaga tcattaccgg
    17101 caccgttgac gacatagaag gaaactccta caggaccgtt acaatcggaa accaggtatg
    17161 gatggccgaa aatctgaaaa ccaccaggtt caatgacgga actgaaattc ctcttgtttc
    17221 tggcaatact gagtggaaaa gcctcaatac acctggatat tgctggtaca acaacgacga
    17281 agccgccaat aaaccagtat atggagccct gtacaactgg tatgccgtgg cctccggcaa
    17341 attatgtccg gcaggatggc ggattccctc aaatgatcag tggaaaaccc tgacaacatt
    17401 tctcgggggt gaataccttg ccggagacaa gctgaaggag gaaggatctg aacactggaa
    17461 acatttcaat gacaagtcaa ccaacgaaag cggattcacg gccctgcccg gaggcagccg
    17521 tattgatgga tcttacgtcc tgatcggaga aatgggagcc tggtgggcat caacaccgtt
    17581 cagtgaggaa gaggcctatt taagagaact ggatgatttc gtaacagaag tattgtccgg
    17641 gtatctgaat aaaaaatatg gattttctgt cagatgtata aaaaataact gaggcttaag
    17701 cataatcctg gcagtaaacc aatgtccgta ttaggaatcc gcccggtgaa atcttttgaa
    17761 ataataaacc ggaccggtat caggctgtcc ggtaacggat aacaattatc agatcattga
    17821 attgactttt tcggcaactc tttttgcaat atccgaacat agtccgatat aattccctga
    17881 aagatagttt tcagcatcac ccgcaactga aaagttggca ccgcagatat cgaggcaggt
    17941 agttgatccg aattccttgc ggaaccagat tcccaggttc tcgctgaaat ggatggagcg
    18001 gttgaaattg ttcatgtcct catccatcgc cttattattg tttgccttaa acaaccggtt
    18061 catccttgca accctgaaaa agtttttttc aatctctgcc gttactgagc tcagagccat
    18121 gacaccggca gcaagggccc cgcaggttct gttcagcatt gcgagtcctc ctaccagaag
    18181 ccatgatgca tcatacaatt cctctgccgg cctgaatttt ccatcgagat ttttcagcac
    18241 attgccggca caatgaaaat tccgggaatc gaattccgaa agcaggatgg catatgattg
    18301 acgcgcaaca tccgggacag gataaggttt tttcacagag gctgaataca acccgtaaaa
    18361 attacttacg gccctcctgc atgccggcat tcctccctgc ctgatatttc cgcaaataca
    18421 ggtcccgttg aactcttcaa atcgtttaat gtaatcctgc cccctgctta tcagttcaag
    18481 cttttcagat aatccggcag gttttttctc ctcaaatccc atgtaaatca aaggcgctgt
    18541 cagggctccg cattccatgg aagggcccgc aatccctccc gacattgcac cggtgtacaa
    18601 aaccaggccg gaatgccctg ttctgccggt ttccaccaga gtctgcatca cagatggtgc
    18661 acaatggccc atcctgtaat agttcttcag gctcctgccc tttcccgtgc taagcaaacc
    18721 ataaccaccc agcccaaccg tagcaagtgc tgccgcagct gtcagacctt tccgggaaaa
    18781 atccctcctg cttataattc tgccttcttc cctatccgtc atcatgacaa tccatcttaa
    18841 ttattacatt taaatcaagg cacttcatcc tggtcccata agatagcggt aattcgggaa
    18901 accagcaaaa gaaccctatc tgcccggccg gaagagttca aacatattct atctcttacc
    18961 agctgcatct caaatttagc cataaaacaa atatctgaac agactggagt ggttaaaaat
    19021 atgaggctgc atcaactggt taatagaagg atatataccc ctggatcagg gtatcagttg
    19081 agtactgctc agagtattgt catgtagccg gtgagatacg ggaaacattt ccggcaaacg
    19141 accagttttg tcaatgcagc ctcattttat ctgaaataaa ttaaataatc attatgattc
    19201 cctttaattg gaacggcgaa cattccgcga ccagaaaaat gtgacaccgt taatttcact
    19261 catgtcaata atcttgaatc cgtgcgtctt aagaatctcg gataccttaa tccccgacat
    19321 tttctgttcg gtgatgtaac caaacaggtt cctgatgctg cttaacagct ttcctgatga
    19381 ataaccatgt ttcacattgt atgctctttc cgtagcggaa tcggagaaac atccgataaa
    19441 actggcttca ggcggaagga tacgatagag attatgcagg aacctgtcaa gatgcctcac
    19501 aagattgagt tttttcatgt ttacgagtat cttgaggctt ctcaagtcac tttcatcata
    19561 atagtaatgc tgtcctggcg aaagcaccag gatattcggc tcagacaaca ggccaatgtt
    19621 tttaaggtag agaagaaaat cttccctccc ttcagctgtc atgtgcaaaa tcgcctgttt
    19681 caggtcaggc ttcggttgcg gactattctc cttgttcatg tcattgcttt tgatttgccg
    19741 gaaataaaat ctttaaaacc cggtggttca aagtataatt ccatggtaat aaaaatagtc
    19801 ccggataaca agcctgacaa tacccgaata ccggacagac gggaaaataa gacaaacacc
    19861 cggcagcttt atcaatcaaa tcgggctatg cgttcaccat ataccgtcat tgatgaaacg
    19921 tttcagtctc ttcgtcaaaa ggaaccggaa atttacaggc agtttcacga aaacagacag
    19981 gagtaattca attcatttaa tataaatatt gatattcaat aacttacgga acagatcaat
    20041 gaattaagca aatacggact cgaaaaatca ttttgaccaa acaattgaat tctatgatta
    20101 aaaaactgta aaaagcatgc tttttacagt tcgggtcaat caggatcagg ctgagttcat
    20161 tcaattcctt cgccccaggc cgaaataatc atccctcagt tatctgtgcc ggtctttaat
    20221 gaatcgtctt tcgggcaatt attcggccgg ataaatggcc ctcatcaatc aaggtaatta
    20281 aggcaggctg actgaaagac cttcaaaact ggcatacagg cctggtttga aatttaacgg
    20341 caatgtgcag gcatattcaa tgacatacgg cttcaaatgt aaacacgatc attttatcct
    20401 gcagattact ggcttttctc cgtgaacttt atatttttgg gaaacattcc gggatcagta
    20461 tccggatgga ttcagtaaca aacacaggag aaaacataaa atacaaatca ggaacctgta
    20521 tcaatacctt cagatcaaac caataaaaga agtataaaaa atcatatcag gatgaaaaag
    20581 aaccactcaa tcacacagat gagatatgcc atgatcattt tgatgatatt gttttcctgc
    20641 ctgggcgcat atccccaggt gaaacttact caggaggatt gggtgattcc cacctgggct
    20701 gttctcccgg cagataagaa tccaatgttt ttcaaaaaag agtcatacca gggtgcatca
    20761 aaagtcatat acccctatgc catgaatgac atgatttcgg atgttaagac cgacaaggca
    20821 tggaaaacca ttaccctgga aaatgaatat atccggcttg cggttactcc tgaaataggg
    20881 ggtaagattt attacgcagc cgacaaaaca aataattaca atttcattta caagaatgat
    20941 gtcgttcgtc catccaacat cgggatgctt ggtgcatggg tttcaggggg aattgaatgg
    21001 tgcgtgctgc atcatcacag ggcctctaca tttctgccgg ttgactggtc cgcatcagaa
    21061 aacagcgacg gaagcaaaac cgtgtgggta ggggaaacag aaccccgcca tcgtatgaga
    21121 tggaccatcg gaataacagt gttccccggc aggtcttatt atcaggctga agttaagatc
    21181 accaatccga caccctacac acattcattc ctttactggg ccaatgttgc ggcacatacc
    21241 aaccgggact atcaggtcat cttcccgcca agcgttcaga ttgcaacttt tcatgcaaaa
    21301 aacagcttca ctcactggcc attttcaaca gaaatataca gcggccagga ttttacaagg
    21361 ggggtggaca taagctggtg gaaaaacgcg gagaattcca attcattttt tgctcacgat
    21421 cttaaggaag atttcatggg cggatatgat catggaagac aggcagggac cgttcatatc
    21481 ggagatcaca acattgtcaa aggtgcaaaa ctctgggaat ggggatcagg tccaagggga
    21541 caggccaccg aggccaggct caccgataaa agcggtccgt atgttgaaat catggtcggg
    21601 gcattttcag acaaccagcc cgattacagc tggataagac cttacgaggt aaaggtcttc
    21661 aagcaatact ggtatcccgt gaaggacatt cagggtttca aaaatgccaa cctgaatgga
    21721 gccgtaaacc tggaggcacg ggagaaaaac caggttttcc tcggttatta ttcaacaagg
    21781 aaagttgcaa aggccaatat cgtactgaaa cagggagaca aaacgatttt tgaaacgacc
    21841 acagaaatat ctcctgaaaa ggccttcacc cggacaataa agccgggggt tccattcata
    21901 atggaagacc tttacacaca aatgtcggac caggaaacag gagaggtgct agtttcatac
    21961 aagccggttg caataaagcg tgaggataag cttccggaga tcgttaaaag accggatgaa
    22021 ccgaaagata ttccgacaat cgaggagctg tatctcgccg gcagccggat acagcaattc
    22081 tacaatccga cccttgatcc catggactat tatacggaag cccttaaaag ggacccggga
    22141 gacataagaa caaacaccgc cgtcggtaat tattatctca aaaacgggga ctacacaagg
    22201 gcaagagcct gtttttcgag ggccataaaa agacttacga aggattacac ccgtccgtcg
    22261 gactgtgagc ccctttatct tcagggatta accttgaaag ccatggggct gtatgacgaa
    22321 gccgttgaca cattataccg tgcaacatgg gatcatgcct ggcattcggc tgcatatctc
    22381 gaacttgcaa gaatttcttc catgcgtggc gactttctca aagcccttaa tgaaactgat
    22441 gaatcactgt caacaaattc gaggaacaat ccggcgattg aactcaaagc ggcactactg
    22501 agaaagaccg gggatctcaa ggcttcctct gaactcctgg aaaccatcgt gaaacaggat
    22561 cctctggatt tcagggcagc aaatgaatat tacctggttt tgaaagcctc cggcgacagg
    22621 caaaaggctg atgccgtgct tgccgacctg gcaggtaaaa tgagggattt cgatcagaac
    22681 tatctcgaac tggcaataag ctaccttaac gatggatttt tacaggaagc tgaagatgta
    22741 ttgaagagat tcaggggcag caatccgatg gtaaactatt accttggata tattctcgac
    22801 aaaaacggca acaggcccga agcactgaag caattcagat caggatcagc actgcccgtc
    22861 gactacgttt ttcctttcag gcttgagact gtaaatgtac tcaggctggt atcatcctgt
    22921 ttgcctgacg acgccaggcc ctggtattat ctgggcaatc tgctttacga caagcagtcc
    22981 gcaacagctg ttgaatgctg ggaaaaggcc gtcagtctcg atcctcagct tgcaatcgcc
    23041 tggagaaacc tcggatgggg atattactat gccatggata atggcgaacg ggcaattgaa
    23101 gcgtatgaaa aagcaattgc acttgaaaag aacgagcctg tttactacac cgaactagat
    23161 gcgctttatg aaatgagcaa tacaccggtt gagaaaaggc tgaggctatt tgatgggtcc
    23221 aatgaaactg tcaggaaacg tgatgattca tttattcgtc agatcgttgt gcttacgctt
    23281 gccggacagc ctgaaaaggc agtggagtat ctggccggaa ggaatttcag ttatcgtgaa
    23341 ggaagctcca gggtacgaga agtactgatt gatgctcacc tgatgatggg aagaaaacat
    23401 atggaagaaa ggaacttcaa aaaagcactg gatgaattca tgcttgcgcg tgttcctgat
    23461 gaggagggtg gaagtgcaag agccggagca cgggaggtac aggtagatta tcatatcggc
    23521 ctggcatacg aggctctggg aaacaagtca aaagcgcgga gctactatac catggcagca
    23581 aaacctgaaa taagggacgg gaactatata agatactatc aggcattgag ccaggcaaaa
    23641 ctcggcaata aaacaaaggc cggggaaatt ttcaattcaa tgatggatgc cggggaaaaa
    23701 cagatcagca gctccggcag cgacactgat ttcttcgcca agttcggtga aagagaagcc
    23761 aggaatgtaa tattgtcgga ggcgtacctg ctaaaggggc ttgcctgtaa aggcctgggc
    23821 aacatcgaac cggcaaagga gaacctggaa aaggcgctgg aactttccgc cggcaacctt
    23881 tatgcgaaag tggaactcat ggacctgaaa tagaaaaccg gccccgatat aataataatg
    23941 gaatatcact tccccgtgat gttcattgat gctgcataat aacggggatc tatgacaatt
    24001 tccgcattga aggttccaag gtcgagctca ttatagtctt ccgtgggatt aatcttcatt
    24061 gccctgcccg aaacaaatat cctgacgggc atattgaagc cgggcacgca attggtccat
    24121 ctgaaattca atttactgcc gtcgatgaaa tattcaagaa ccggaatacg aatatccctg
    24181 aggtactggt caaaaaaagg cttcagatca agtcctgagc gggaacctat ataattttca
    24241 acctgcgatc ccttcacaac ctggtgataa aactccctgt taagtcccct caggatatcc
    24301 ctccattttt catcatcatc aattacctgg cgaagcgtat gaagcatgct ccctcccttg
    24361 taatacatat cgcccgatcc ggtgaaattg acattatata taccagttat tgtcctcttg
    24421 ttgagtattc ttctgcggca ccccagtata tattcgctcg cggcttcctt tccgtaataa
    24481 tattccaggt acaggctttc ggcataattt gtgaagcttt catggatcca catgtcggcc
    24541 gcatcttcat tggtaatgct gttcccgaac cactcatgac ccgattcatg gattatgatg
    24601 aagtcgaacc tgtgacccca gcccgtgcca ctcagatctt cgcccaaata cccatttccg
    24661 tatctgttcc cgtatgtcac cgaactctgg tgctccatcc ccaggtaagg gacctcaacc
    24721 agtttgtaac catcctcata gaagggatag ggtccaaacc agtgttcaaa tgcctcaagc
    24781 atccttgcaa cctgtttaaa ctgagatttt gccttttcaa gattctccct gagtacataa
    24841 taatcacaat cgagcctgcc cttttcaccc tgataaaact cagagaaatg tgaatatcgg
    24901 gctatattga tattgacccc atagttgtta ataggattgg caacaaacca gtggaatgtg
    24961 gtggtcatgt tcctgttttg tgttttggaa cgaagtcgtc cgtttgaaac ggctgtcagt
    25021 tcccggggga cagtaacgct tatcagcatg ctgtctggct catcatacaa atggtccttg
    25081 caaggccacc agaggctcgc accgtcaccc tgacaggaag aggcaataaa tggcctgccg
    25141 ccgccgtctt tcgcccaagt gataccacct tcccaagggg ggcgtttggc aacccgtggc
    25201 ttcccgccaa aatcaaacac aactgagcct gttgacccta caggctgttc ttccaaaagc
    25261 ctgatcaggt a
//
LOCUS       DAOOYG010000029        24167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1907_length_24167_cov_6.480259, whole genome shotgun sequence.
ACCESSION   DAOOYG010000029 DAOOYG010000000
VERSION     DAOOYG010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24167
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1907_length_24167_cov_6.480259"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="PK288_04355"
     CDS             <1..1248
                     /locus_tag="PK288_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPM18182.1"
                     /translation="EVGRQAAMENIADVLEALSGNTKMVFVTTGMGGGTGTGAIPVIA
                     KACKDSGLLTIAVVTIPFRSEGKIRIKYAIDGINELKDHVDSLLVINNEKLREIYGDQ
                     GVSTAFAKADDVLTTAVKGIAEIITVAGYINVDFADVETVMKNSGVAIMGMGSASGEG
                     RALAAIEKALASPLLNSNDITGAKSILINISSGKGANELTMDELGEITDYMYKTASED
                     ALIIRGLSQDESLNDSISVTVIATGFPATSIFTPYKHAKPKKVELLALNNPVPPHIIP
                     EKGDELFTIRDKKPREIVSAIDDTVQGELDFSMEKVEQITEIRPPRPAESETEREKPE
                     TTLFRVKHMQNILKKEGLSNKIMKENIETFEDVPAYIRRNMSLRTAEKTGESKISKFT
                     LSSGDEEGPVLRENNAYLNDNVD"
     gene            1552..3474
                     /locus_tag="PK288_04360"
     CDS             1552..3474
                     /locus_tag="PK288_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HPM18183.1"
                     /translation="MSKALISGLVFLFSAALYAQTQDTIKPRTITQYNLSGDFSGEIT
                     IPFDTVFSLFHRYRHADRYSPVNATLGNYGLPFYQISFFDRIEDPDKYLVRYYYPLVY
                     QPENYIFMNTQKPFTELVWTYGGAREKAEQTFRIRHSQNVNRFLNFGLDYKIIYSLGQ
                     YSYQRSNDKDFTFFTSYRGEKYKMYMAWGLNHLRAGENGGISNPDQLKEYETRDVSVS
                     LGRLNSAKSYLRNWNMMLVNKYTPGKIRPDVTDTTGLKKRSFRLSGTFSHILLIDNNR
                     RTYSDDSPGTGFYDSIYISNALTFDSLSARSIKNTIRFDFVTDTTRKVRLGGGVGLRN
                     EMFRYGQIIPTHIDTMAADTASWRKTGTVLVGRLYNNIGDKFNWSASGELFFSGYRAG
                     DFILDGVINKVFDLRKGRALLTLRGSMTNRTPSIWFTHWGSNNFEWQNSFNRIFRMNL
                     GAGVSFPARNLEMKFNYAIIDNYTDFDTLALPSQHTGGLSVASLHIRKDLRAWKFHLA
                     SDVLIQKSSNAEILDLPLVTVKSAGYFEHLFRFPRTNGALNTQLGADVTYHTLYNSYS
                     YIPATGIFHRQDKIKTGNYPFINVFLNIKLKRTRIFIMVDHVNSGLMGYEYYMVPFYP
                     QNVRMFRYGIAWTFYD"
     gene            3518..5023
                     /locus_tag="PK288_04365"
     CDS             3518..5023
                     /locus_tag="PK288_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPM18184.1"
                     /translation="MKLTATGSLTRHLSFLIIAALMLSAASCATRSGKLHRRDTVSID
                     LDSIRARGKLIALTDFNSTNYFLYRGEPMGFQYEMLQALSKHLGVGIEIVTENRIDKA
                     IDMLHTGRADILAMNLAVTPPREKEIRFTEPISQTRHVLVQRKPDGWNEISAGEVENS
                     LIREPSGLKGRTIYVQEHSSHAWYLRSLTKETGRAFSVKEFPFESEELIQLVDNGAIE
                     LAVCDEDIALVNSTYYPAIDVNTPLGPVHEVSWGVRKTHSDQLLAAIDEWIVSFRKTN
                     TYALLYAKYFRNPRSGAIAKSNYFTLTTGRISPYDDLIKTYSDTIGWDWRLLASLICQ
                     ESRFNPHVRSRVGAYGLMQIMPRTGKNFGIDITSSPGNNIYAGVKYINWLHSIFDPKI
                     PDVHERVRFILAAYNAGPGHILDAMNLAAKFGYDPLVWKDNVEKWLQKKSESKYYSDS
                     SVKNGYFRGIESVAFVNEILERYEHYRNIVSENKYAISSSGTANWPGRRQF"
     gene            complement(5073..6317)
                     /locus_tag="PK288_04370"
     CDS             complement(5073..6317)
                     /locus_tag="PK288_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM18185.1"
                     /translation="MNLPYFIANKLIRGRRKGMSFSRPINIIAIIGIASGLAVMILAV
                     SILTGFKQQIRDKIVGFGSHIQVLNFDSNISFETAPISDTQPFVPAIRNIPGIRHLQV
                     FATKAGIIKTGQDIQGVVLKGVDSDFDWSFFKSNIVAGSTFTVTDSVRTNDVIISKRI
                     SDLLKLRTGDSFAMHFIQDPPRSRKFTICGIYETSLEEFDRMYVFCDIGHIKRLNGWS
                     NDQVSGFEIFIDDFRKLDEMTMAVRDAIGYRLTEDDTKFKVTNIRVRYPQIFDWLNFQ
                     DMNVIIIITLMLIVAGFNMISGLLILILEKTNMIGILKALGSENKMIRKIFLYQAAYL
                     IGKGLFWGNIIGLGLAILQLRTGLITLDPSSYYIKTVPVNLDFIHILLLNAGTMAAII
                     TMLLVPSQLISRITPVKAIRYD"
     gene            6582..7754
                     /locus_tag="PK288_04375"
     CDS             6582..7754
                     /locus_tag="PK288_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPM18186.1"
                     /translation="MTKVVNIVSFAVMLLSAGSCIYAGGDPVPGAERAGEYIHLIKGR
                     KIGVVANQTSMAGKVHLVDFLVGSGIRPVVIFSPEHGFRDMADAGEKVADGRDPSTGI
                     PLTSLYGSHLKPTPADLAGLDAVIFDIQDVGARFYTYISTLHYVLEACAENNVRCIVL
                     DRPNPNGFYIDGNVADTLHRTFVSMDPVPVVHGMTVGEYAMMLNGEGWLKNGVRCDLT
                     VIPCENYTHETLYELPVKPSPNLPNQTAIYLYPSICFFEGTNISLGRGTDFPFQVFGS
                     PNLPDRGFSFTPRSVPGAKNPPLLGVKCFGTDLRNAMADKTVPAPKLNLEWIISAYRD
                     YPDKDKFFTPYFDVLAAGPVLREQIQKGMSAEMIRQTWQEDLAKFAGIREKYLLYR"
     gene            complement(7931..8302)
                     /locus_tag="PK288_04380"
     CDS             complement(7931..8302)
                     /locus_tag="PK288_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM18187.1"
                     /translation="MDKNLTFEEIKACPVRCVLAINDTLNVITGKWKLPIIGSLLYNK
                     KRFTEIQQNIPKITPRMLSRELKELELNGMIKRTVVSTTPVTVEYELTDSGLKIREVL
                     DKMLQWGIQHRNNHFTSDSSG"
     gene            8445..9047
                     /locus_tag="PK288_04385"
     CDS             8445..9047
                     /locus_tag="PK288_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPM18188.1"
                     /translation="MKKREFIKKGFLGIGSFFLAGKAKGLEYYPMPSDKRIAVIYATW
                     CGTSRDAALWISEGMDGIANVFDVREDPDISGYDHLIIGGSIRQNKVPGQLENFLEKN
                     RAAVSGKVRGLFAVCGNMRQPVGNEQYRTFIQDYLAPLSGAGQVPSRIFLGRITWGLM
                     EPEVCEMMKKIPGMEEYDNLKRPECMAFGREILDAINKVN"
     gene            9090..11525
                     /locus_tag="PK288_04390"
     CDS             9090..11525
                     /locus_tag="PK288_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPM18189.1"
                     /translation="MKKLLLLLIIVFPLVSVYSQIDDKLISDIKNSGYVHRPLPLDHS
                     KSYEAFAITKKVLVSDMLCDMEDLSKWTHSGVGGMRLTSERSVSGKHSLRLVAPAVPD
                     KYPGWGIGRGSSVASYDVGGRNLEKYNRVRLRVFPDCEGARSIYLNLLFENDGKEKVP
                     DPFGREGIHEMNLVNGQWNDCVVEIPELARDKVTKISLAIEIFGREQTMGDSLKFDID
                     AIELQVVEKPEVVSGWIPAEDRIIFSTTGYGLNSEKTAIVRTSENVSRFQLVDKATSR
                     VAYEGNITQTKTRLGEFKILNFTDFRDVGEYFLRVGKIETQPFFINKRIWENSMWRVL
                     NFVFCERCGYPVPDKHGACHGDHHAVFNDKIFPVHGGWHDAADMSQQSLQTAEVAFSL
                     FEVANNAKRNNNHDLYNRALEEAEWGLDYILKTRLGGGFRVQNWGTNLWTDGYLGTDD
                     DKGWHQAGVHDSGFENFLFSGIEAYAALSISHDEMLKGHLIKAATEDFGFALKRFEEK
                     GYAEMVVDWGGHAYMASESQYMASVSWAASQIYRLTGDKYYADKAAEFIQYVLKCQRT
                     EPLNDKDKLRGFFYRDLGKKSIVHFNHQSRDQVFMQALTELCLTQPGHKDYGKWENSI
                     RIYGEYLKKLMSYVAPYGMLPSGVYHKDEVKDTINFFRWQVGRSKEPEKDYMEQLNNG
                     VKLDNEHYLRVFPVWFSFKGNIAVHLATGKAAALCGKFLNDKELINIAEQQLCWVVGK
                     NPFGQSMIWGEGSNYAQQYTALPGETVGEIPVGMQSKFNGDIPYWPNINTAVYKEVWS
                     SSAGKWLSLVAEF"
     gene            11784..14018
                     /locus_tag="PK288_04395"
     CDS             11784..14018
                     /locus_tag="PK288_04395"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPM18190.1"
                     /translation="MTKPIILLAVSLLIITTKIFPGEAVNIKRIKGEILFDGIPDEPE
                     WANLTYLPMTMHSPVFGAEPSEASEVRIGYDDHFLWIGASLYMNDPGKIFAVTRKRDD
                     RLFGHDAFGIILDTYNDKENALVFYTSPNGIRTDFTISNDAVMNGGGFGLNSSFNISW
                     DTFWDVKTRIDNKGWHVEMKIPFSSLKFKPSGDVTTMGLIVCRNISANNETDTYPAID
                     PKYGMIASNKPSLAQEIIIEGVKPSKPVYISPYLLGGGTREYNLNELQTGYVRNEKPE
                     YNAGLDIKYNINSNLTLDLTANTDFAQVEADDQQVNLTRYSLFFPEKRKFFQERSSLF
                     DFSLGGFSDNMFYSRSIGVFNGSPVRIYGGARITGRMGRWDTGFLNMQTGAHDSVPGE
                     NFGVLRMRRQVINQNSYVGGIITSRLGMNGYKNFGYGIDGIFRLFGVDYLNVKWAQTY
                     DSNLENKMNSLDPSFILVDWERRNEKGFAYRFNYTYSGEQLKPGVGFVMRGAVQGLNG
                     RLSYGWFPGEKSRIYNYSVSVMAEQYNRLDDGNLESAMITPGINIGTKKGINAGLSAE
                     VRKEGVRNDFYLSENVVVKANEYTFTNGQMMFSTPNSKMISVMTGINAGQFYDGLRYG
                     TMTMVNFNISSGFNLSVNYDFNAIRFPDRASNNSLNIHLVNAKALVMFSTKLTAAVMV
                     QYVNSYDNLLTNFRLRYNPREGNDLYLVLNDNRRISGSGSVPEYPRFFNQTVMIKYIH
                     TFIL"
     gene            14325..14888
                     /locus_tag="PK288_04400"
     CDS             14325..14888
                     /locus_tag="PK288_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HPM18191.1"
                     /translation="MSEERDLIQRISRGDTDAFRELFAACYRSLFSYALSLVIDRETA
                     KDIIQDVFFRIWENRKNFEIHTSLKAFLFASVRNSAIDHIRKQKRFSGYERELVEILS
                     GQTSSGNAAPEPLNSIIYNETCDIVESAIEKLPEQCRRIFIMSRFHGLKHKQIAEKLD
                     ISSRTVEAHIYAALKFLRETVKKSLSD"
     gene            14933..15976
                     /locus_tag="PK288_04405"
     CDS             14933..15976
                     /locus_tag="PK288_04405"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4974 domain-containing protein"
                     /protein_id="HPM18192.1"
                     /translation="MDPSVNIEHIFLNLLEGGEISAEELKILHAWLESNPDEAEKYAD
                     FREIWALASVPENMAESECREGYRQLEKRLRAKRWPVIRMEKQRRLRVPVLAAAVIAG
                     MVIAIGALHLWDSRIIRKERFVVEVPAGSRSVTILPDGTKVTLNGDSKISYDQGYGRS
                     NRNVDLSGEGYFEVVHNARHTFSVCAAGAMVAAHGTVFNVKAYPEDPSVETTLVKGSV
                     SIEIPGHRKERMFLKPSEQFTWLKPVVADDSDKGRVLISKGIDTERYTSWISGRLIIQ
                     SELLADLSLRLERKYDVEISFEEQDLKNLRFTGVLEDETIEQVLEVLKITSNLDFKIS
                     GREIVLFSCRKTP"
     gene            16001..19414
                     /locus_tag="PK288_04410"
     CDS             16001..19414
                     /locus_tag="PK288_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM18193.1"
                     /translation="MNLTYRKILKNWRKAAFLALLMLPGLNNMWGQETRLNLPSTTMT
                     LAELFKSVQDKSEIRFFYNDDLINPDQRVTLSSTKLTVAEMLDELKRQTSLSFRLMGS
                     NMIAVTAGTQARDIQLSGRVTDSSTGEPIAGVTVTLRGTNIGVLTDINGKYSVSVPGN
                     VEIVAFSFIGYNTVEIPVAGRSVIDVSMEEGIQALDEVVVTALNVNRTKSSLGYSIST
                     VKGADIEKSGNNNMINTLAGKVAGLHISRTAGGVDGSSRVILRGVMSITKDSRPLVIV
                     DGIPVDAGHSSGGRWGGSSGGDALGDINPDDIENISILKGAGAAAAYGSRGANGVILI
                     TTKKGAESKGLGITVNSSIVAETPLLLPRLQNKYGHGSFGTYPASVPDDGFPWGWSWG
                     PEMKGQMVRGYAGDMVPFSPQPGNFRDFFRTGSMQANTVTIETGTPESSVRASFSTQN
                     NKGIVPRNNLTRQTINLRGFSRSRDILELDGKIAYMHSKITGRPEVAEGPGNPAYYLS
                     QAPRNISNDYLRNNYEDDSGKELLWTKDVYTGNPYWQLYNKDNEEEKHRVQGVFSGKI
                     IVMPGLDITARSGLDMTYRLNHSDLAKGSICSGNVNGSVYNSISNFLEWNSDVLANYS
                     NRLGEDLKYTVNFGGNYRYNYSRGIGQYGSNLKINKFYAISNCWSYSTNEWLSKKEVF
                     SVYSLGQVSFREWLYFDVTLRNDWSSTLPPGSNSYFYHSENLSFLFTKALNTDTRILS
                     SGKLRASFAKVGNDTNPYETNQYFYVSQSQLPYPFGTFSDVLPTYDLQPELTHSWEAG
                     VNLEFLNSRIILDVTAYTTNSNNQIIRVQLPVSSGFNRKVMNAADLRNRGIELQADFS
                     VIRSRKFNWDMTLTWSKNKTMVKELYKNASSFLLDETWFATIQAVPGEEYGLIYTRDF
                     KRDAFGRVLVDDNGFVLAGEYKRMGKITPDWTGSISGKVSYGNFSLAALVDIRMGGNV
                     YSIGKAYRCLFGTSAETLTGREEWYSTHDPEYLYSVPLPGIDPNGYIEKGFNENTGQP
                     NAAPVDPMYRWYNIWSKEIGTEWLLDATNARLREISLTYSVPAGILKNFPAKGLSVSV
                     IANNLLCIYNAMKDIDPESGYSSGNVGGGFEHSALPSTRTYGFNVKIDF"
     gene            19434..20933
                     /locus_tag="PK288_04415"
     CDS             19434..20933
                     /locus_tag="PK288_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPM18194.1"
                     /translation="MKRSVKSFVCPVLITLLVLLTACDKRLEEINRDPLGLDDLSDEY
                     FFNTGLRMAFGDYFWLRSFQMRFASQYSHVYVTSTEMRNADSYEDFHSQDVYKEMFQA
                     AYTGPAVYMNKVVSMTAGGKHENNVRNAIARIVSAVIFATITDCYGDVPYSEGAKGMD
                     GILYPKYDSQEFIYSDMMEQLKTAIEVLKTADPSRGYVNADPVYDNDLSKWVRFANSL
                     RLRLAMRSRFASPAKSAAIITECFANSFIETNDQNFAWKHQDSENSELYNPWFDVHRN
                     EDFRMSEKFVDWLSSTNDPRLEILVSPVPGRGYVGIPNGLSSLQVGNYPWANYSKPSP
                     ALYARDLPQTLLCASEVWFLRAEAALFGLAPGDPNEFYREGIIRNLEFWNADPAETNT
                     FLTDEPEATLSGTIENMFRQISTQMWIAFVPNFVEAWSNIRRTGFPVIPQRTNAEIFS
                     LGVTNGILPKRFKYATNEYLTNGINLQAAIDRQGPDKIDTPVWWDVLDR"
     gene            20996..21907
                     /locus_tag="PK288_04420"
     CDS             20996..21907
                     /locus_tag="PK288_04420"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM18195.1"
                     /translation="MITAIFAVMGVSCKKTPAPTAEIFATIDSYTVTFNPTVSDVKTY
                     DWDFGDGEKSTEAAPVHTYKKSGTYTVRLTVKGDGGEISVTKDITIAASVEEMLTGGP
                     SAVNGKTWVLSTSYTAGQDGGGPVQNAMPITIPSAENVLDLFGLGEEYDNEFTFFSDG
                     SYKMTPKNGKVLAGAVYGVATGTISGDPVYEIGMCAATFDPPATATWSLGTTDFIVDA
                     ITDPDETNVPPVHADVTFTGKTWVKLSAGAYFGVLDFPTIDRFIIKSITPSKMTVALF
                     LCGYGYGDDPEMIMLPTNLIQLTYVPK"
     gene            22008..23633
                     /locus_tag="PK288_04425"
     CDS             22008..23633
                     /locus_tag="PK288_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPM18196.1"
                     /translation="MKQLIRTVICLALAFTACKQANLPKEYEFGVIVNNGITVDGQKF
                     IDSYGRQVILSGINKVNKNPNDNYLDRDSAESYRQMKEWGFNVIRLGVIWDGIEPEPG
                     IYNEQYLDEIEKRVNWAAENGLYVMLDMHQDLYGVSMSVKDPYSGDGAPEWATITDGL
                     PHHKGEIWSDSYIISPAVQKAFDHFWANTPASDGIGLQDHYALMWKHIAARFAGNSAV
                     IGYDIMNEPFNGSQGIPVLPAILREFAGVYAEATGTVQDEEQVMTMWASEENRMKAFS
                     YIEKADVYARVIDAATEINQQFERNELRDFYQKVGSSIRQVDTTHILFLEHGYFANTG
                     MRSALEPVRHPDGTIDPLVGYAAHGYDLLTDTKNVGSQGAERISFIFSRINETGKRMN
                     SPVLIGEWGAFGGNAPGLVAVARNILKLFEEYHFSNTFWTYFNNIQNYSFFTGAIIRP
                     YPQFISGTLSSYSFSHETGDFNMTWQEDPAVKAPTVIFIPSLDRLVKESVRVSPGNDR
                     IIIETIKGTTNGYIVISPLGGAQERNFRLNLSY"
     gene            complement(23770..>24167)
                     /locus_tag="PK288_04430"
     CDS             complement(23770..>24167)
                     /locus_tag="PK288_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPM18197.1"
                     /translation="RNMMAVGVKPSKIVLGTAFYGKGRVVKTTDNNGLYQIPVPVWRW
                     LGGGYSYLKDSVVNKKGFTRYWDAESKAPYLFNADEKAFITYDDEESTRIKCEYILKN
                     KLAGIMFWEYFADQKHYLLNEINKSFGYK"
BASE COUNT         6858 a         5404 c         5798 g         6107 t
ORIGIN      
        1 gaagtgggcc ggcaggctgc aatggagaac atcgctgatg tgcttgaagc cctgtcggga
       61 aacacgaaaa tggttttcgt tactaccggc atgggaggcg gaaccggtac gggtgccatc
      121 ccggttattg caaaagcctg caaggactcg gggctcctta caattgcagt ggtgacaatt
      181 cctttccggt cggaaggaaa gatcaggata aagtatgcaa tcgacggtat caacgagctc
      241 aaggaccatg tcgactcact acttgtgata aacaacgaaa agctaaggga gatctatggc
      301 gaccagggcg tttccactgc atttgccaaa gccgatgacg tgcttacaac agctgtcaag
      361 ggaatcgccg aaatcatcac ggttgccggc tatataaatg tcgactttgc agatgttgaa
      421 accgttatga aaaactcggg agtggcaata atgggaatgg gatcggcctc tggggagggg
      481 agagccctgg cagcaattga aaaggccctc gcctcaccac tcctgaattc gaatgacatt
      541 acgggtgcca aaagcattct tatcaacatc tcctccggaa agggcgcaaa tgaacttacc
      601 atggatgaac tgggggaaat caccgattat atgtacaaaa ctgcttcgga ggatgcactc
      661 attatccggg gcctttccca ggatgaaagc ctgaacgaca gtattagcgt tacggtaatt
      721 gcaaccggat tccccgcgac cagcattttc accccctaca aacatgcaaa gccgaagaaa
      781 gttgaactgc tggccttaaa taacccggtg cctccgcata taatacccga aaagggcgat
      841 gagcttttta caattcgtga caaaaaacca cgggaaatag tctcggcaat cgatgacact
      901 gtccagggtg aacttgattt cagcatggaa aaagttgaac agataaccga aatcaggcct
      961 ccacgaccgg cagaaagtga aactgaaagg gaaaaacccg aaacaacgct ctttcgggtg
     1021 aaacatatgc agaatatcct caaaaaagaa ggtctctcca ataaaatcat gaaggagaat
     1081 atcgaaacct tcgaagatgt gccagcatac atcaggagaa acatgtcgct caggacggca
     1141 gaaaagacag gagaaagcaa aatatcaaaa ttcaccctgt cctccggcga tgaagaagga
     1201 cctgtgctca gggaaaacaa tgcctacctg aacgacaatg tagattaggg aacggcccgg
     1261 ttactccctg caaagttcag accctcaggc agccaggtaa gacgggatat cacggatcat
     1321 ctccacctga accctggtac gaacctgttc gctgtaactc ccctgcagct gactggtttc
     1381 aatcagatca ttattatttg atgtgaatac attgacccgg gagggagaat gataaaatta
     1441 attgatatat atcaattaat taggtgatga atatcaacga atcataactt caacttcata
     1501 ctggcaagcc acctgtaaat aacgtcttca ttattcatat atttgccggg tatgtcaaag
     1561 gcactcattt ccgggttggt ttttctgttc tcggcagctc tttatgccca gacgcaggat
     1621 actataaaac ccaggacaat cacccagtac aatctttcag gagatttttc aggggagatc
     1681 acaataccgt tcgataccgt cttttcactt tttcaccgct atcgccatgc cgacaggtat
     1741 tcacctgtaa atgcaacgct tggcaactac ggtctgcctt tctatcagat cagctttttc
     1801 gacaggattg aagatcctga caaatacctt gtcagatatt attaccctct ggtatatcag
     1861 cctgagaatt acatttttat gaatacgcaa aaacctttca ccgagctggt atggacctac
     1921 ggaggcgcaa gggaaaaggc tgaacagaca ttcaggatca gacactccca gaatgtaaac
     1981 agatttctga atttcgggct tgactataag ataatttaca gccttggcca gtacagttat
     2041 cagcgttcga acgacaagga tttcacattc tttacttcct acaggggaga gaaatataaa
     2101 atgtatatgg catggggcct gaatcacctg agggcaggcg agaacggagg aattagcaat
     2161 cccgaccagc tgaaggaata tgaaacgcgc gatgtgtctg tctctcttgg aaggttgaat
     2221 tcagccaaaa gttacctgag gaactggaat atgatgcttg tgaataaata caccccgggg
     2281 aagatccgtc cggatgtgac tgataccact ggtttgaaaa aaaggtcttt caggctcagc
     2341 ggaacattct cgcatatact ccttattgac aacaatcgca ggacatactc cgacgattca
     2401 cccggaacgg gtttctacga ttcaatttat ataagcaatg ccttaacctt cgattcgctt
     2461 tctgcaagga gcataaaaaa taccatcagg tttgattttg ttacagatac cacgcgtaag
     2521 gtcaggcttg gcgggggtgt cggacttcga aatgaaatgt tcaggtatgg ccagataatt
     2581 ccgacccata tcgacacaat ggcagctgac actgcttcat ggcgaaagac cggtactgtc
     2641 cttgtcggac gcctatacaa caatatcggc gacaaattca actggtcggc ttcaggtgaa
     2701 ttgttcttca gcggttacag ggcaggtgat tttattctcg acggggtgat aaacaaggtc
     2761 tttgatctaa ggaagggaag agccctgctg accttaaggg ggagtatgac caatagaaca
     2821 ccgtcaatat ggttcaccca ttgggggagc aataattttg aatggcagaa cagctttaac
     2881 aggattttca ggatgaacct gggtgccgga gtctcatttc ctgccaggaa tctggagatg
     2941 aagttcaatt acgcaatcat tgacaattat acggattttg acaccctggc gcttccttca
     3001 cagcatacgg gaggtctttc agtggcctca ttgcatatca gaaaggatct tcgcgcatgg
     3061 aagttccatc ttgcatccga tgtgctgata cagaaaagca gcaatgcgga gatcctcgac
     3121 cttcctcttg ttaccgtaaa atcagcagga tattttgaac atcttttcag gttccccaga
     3181 accaacgggg cactgaatac tcagcttgga gccgatgtta cgtatcatac cctttacaat
     3241 tcctactcct atattcctgc aacggggatt ttccataggc aggacaagat taaaaccgga
     3301 aactatcctt tcatcaatgt tttcctgaat atcaagctta aaaggaccag gatctttata
     3361 atggtcgacc atgtcaattc gggcctcatg ggatacgagt actatatggt gccgttttac
     3421 ccccagaatg tcaggatgtt cagatacgga attgcatgga ctttttatga ttgaaaaatt
     3481 gtggtatctt tgcaccaatg aataaaacag gcatcatttg aaattaacag caacaggatc
     3541 cctgacaagg cacctttcct tcctgataat tgctgcattg atgctttccg cagcatcatg
     3601 tgccaccaga agcggtaaac tgcacaggag agatacggta tccattgacc ttgattcaat
     3661 cagggcaagg ggaaagctta ttgccctgac cgatttcaat tcgaccaatt attttttgta
     3721 ccggggagaa ccgatgggat tccagtacga gatgctccag gctttgtcga agcatctcgg
     3781 agtcggcatc gagattgtta ctgagaatcg tattgacaag gcgattgata tgctgcatac
     3841 cggcagggcc gacatccttg caatgaacct ggctgttact ccgccgaggg aaaaggaaat
     3901 cagatttact gaaccaatca gccagaccag gcatgtcctg gtacagagaa aacctgacgg
     3961 atggaatgaa atttctgccg gtgaggttga aaacagcctt atcagggaac cttccggact
     4021 taagggcagg accatctatg tgcaggaaca ttcctcccat gcatggtatc tcagatcgct
     4081 tacaaaggaa accggccggg cattttcagt gaaagaattc cccttcgaat cggaggaact
     4141 catacagctg gttgataatg gagcgattga actggcagta tgcgatgaag atattgcact
     4201 tgtcaattca acatattatc ccgcgatcga cgttaatact cccctggggc ctgtccatga
     4261 agtttcatgg ggagtgagga aaactcactc ggaccagctc cttgccgcaa ttgatgagtg
     4321 gattgtgtcg ttcagaaaga ccaataccta tgcattattg tatgccaagt atttcaggaa
     4381 tccccgttcg ggtgccattg caaaaagcaa ttatttcacc ctgaccacag gcaggatctc
     4441 accatatgat gatctgatca agacctacag cgataccatt ggctgggact ggcgccttct
     4501 ggcatccctc atctgccagg aatcgaggtt taatcctcat gtcagatcaa gggtaggggc
     4561 atacggcctg atgcagatca tgccacgcac cggtaaaaat ttcgggatcg acattacctc
     4621 ctcccccgga aacaatatat atgccggtgt gaaatacata aactggctcc attcgatctt
     4681 tgatccgaaa atccctgatg tgcatgaacg tgtgcgtttt attctggctg catataatgc
     4741 cggtcccggg catatccttg atgcaatgaa ccttgctgcc aaattcggat atgatccgtt
     4801 ggtctggaaa gacaatgttg aaaaatggct ccagaaaaaa tccgaatcga aatattacag
     4861 cgattcatct gtgaaaaacg ggtatttcag gggaatagag tcggtcgcgt ttgtaaatga
     4921 gatccttgaa aggtatgagc attaccggaa tattgtgtct gaaaacaaat acgccatttc
     4981 ctcatcggga actgcaaact ggcccggccg caggcagttc tgaacctgcc ggtccggacg
     5041 tatgaaaata atcagtccct gcctttttaa attcaatcat atcttattgc cttaaccgga
     5101 gtgatcctgc tgatcagctg cgatggtaca agcagcatgg tgataatggc agccatggtt
     5161 cccgcgttaa gcagaagaat gtggataaaa tcgagattca caggcacggt ttttatgtag
     5221 tatgacgagg ggtcaagggt tatcaggcct gtgcgcagct ggagaatggc cagtccgagc
     5281 ccgataatat tcccccagaa aagtcctttg ccgataaggt acgctgcctg gtataaaaag
     5341 attttcctga tcatcttgtt ctctgaaccc agggctttga gtatgccgat catgttggtt
     5401 ttttcgagga tgagtatcag caggccggaa atcatgttaa atcctgcaac gataagcatg
     5461 agcgttatta ttattatcac gttcatgtcc tggaaattga gccagtcgaa tatctgcggg
     5521 tacctgaccc tgatgtttgt aaccttgaat ttggtatcgt cctcggtaag cctgtaacct
     5581 attgcatccc tgacggccat ggtcatttca tcaagctttc tgaaatcatc aataaagatc
     5641 tcgaatccgc taacctgatc gttgctccag ccgttcagtc ttttaatgtg acctatgtca
     5701 cagaaaacgt acatcctgtc aaactcttca aggcttgtct cataaatgcc gcagatggta
     5761 aatttcctgc tcctcggcgg atcctggatg aaatgcatgg caaatgaatc gcctgtccgg
     5821 agcttcagca ggtcagatat ccttttggat ataatgacat catttgtacg aactgagtca
     5881 gtaaccgtga aagtactgcc tgccactatg ttgcttttga agaagctcca gtcaaaatcg
     5941 ctgtcaacgc ctttcagtac cactccctgg atgtcctgac cggttttgat tatcccggct
     6001 ttggttgcaa agacctgcag gtgtctgatc cctggtatat tccttattgc aggcacaaac
     6061 ggctgggtgt cgctgatcgg ggccgtctca aatgatatgt tggaatcaaa gttcagtacc
     6121 tgtatatggg aaccgaatcc gacaatttta tcccttatct gttgcttgaa tccggtcaga
     6181 atcgagacag ccagtatcat gacggccaga cccgatgcaa tgcctattat ggcgatgata
     6241 ttgatgggcc tcgagaacga cattccttta cggcgccccc tgataagctt gttggctatg
     6301 aagtacggaa gattcataga atcagtcaag tggtcaaatt taataaattc tttgacaaac
     6361 tcctgagaat caaaaaagat aaaccatcgt agcgatgttc acggctaaga ttgaaattgc
     6421 acatttacgg caggggacag gaggaaatcc ccgaatggcg ccttcctgca tgggcgactg
     6481 gcatgaatac tgcaaggacc ggcgggctta attgtacttc aggaaggcag tttcagtcat
     6541 gcaaaaaaaa cattagattt acggaaacaa atcagtaagc catgacaaaa gtggttaata
     6601 ttgtttcttt tgcggtaatg ctgttgtcgg ccggatcgtg catctatgcc ggcggggatc
     6661 ctgttcccgg cgcagagagg gccggtgagt atattcattt gattaagggc cgcaaaatcg
     6721 gagttgtggc caatcagact tccatggccg ggaaggttca ccttgtggat tttctcgttg
     6781 gcagcggcat aaggcccgtg gttatttttt cacccgaaca cgggttcagg gatatggccg
     6841 atgcaggtga aaaagttgct gacggaaggg atccttccac cggtattcct ctcacgagcc
     6901 tgtacggatc ccatcttaaa ccgaccccgg ccgatcttgc aggacttgat gcggtcattt
     6961 tcgacattca ggatgtcggc gcccggttct atacctacat ttcaacactg cactacgtac
     7021 ttgaagcatg tgctgaaaat aatgtcaggt gcattgttct tgacagaccg aatcccaatg
     7081 gattctatat cgatggaaat gtagccgaca cactgcatcg cacgtttgta tcaatggatc
     7141 cggtaccggt ggttcacgga atgacagtgg gtgagtacgc aatgatgctc aacggggagg
     7201 gttggctgaa aaatggcgtc agatgcgatc ttacagtgat cccgtgtgaa aactacaccc
     7261 atgaaacact ctatgagctg cctgtgaaac catcacctaa cctgcccaac cagacagcca
     7321 tatacctcta tccctcaata tgtttttttg aaggtacgaa tatttccctt ggaagaggta
     7381 ctgatttccc attccaggtt tttggatccc ccaatttgcc cgaccggggt ttcagcttta
     7441 cgccccgaag tgttcccggt gccaaaaatc ctcccctgct cggagtaaaa tgctttggca
     7501 ctgacctgag aaatgcaatg gccgataaaa ccgtacctgc cccaaaactc aatctcgaat
     7561 ggatcatttc tgcatatcgc gattatcctg ataaggataa attttttacg ccttattttg
     7621 acgtattggc tgcaggtcct gtcctgagag aacaaataca aaagggaatg agtgctgaaa
     7681 tgatacggca gacctggcag gaggacctgg caaagtttgc cgggatacgg gagaaatacc
     7741 tgctctacag gtaaagtaat tctatcatcc ttaaaataaa caggttccaa ttgatctccg
     7801 tcggggaatg tcggctgact ggtgctattc cggcagatat aatgagatct ttcttaacga
     7861 ttccgggttg ggaaacctcc cggaaacacg gagcatacac gccggcccgg ctcctgtgac
     7921 tggtgaaatg ctaaccagag ctgtcggatg tgaaatggtt gttcctgtgc tgaatacccc
     7981 attgcagcat tttatcaagc acttccctga tcttcagccc cgaatctgta agctcatact
     8041 ctaccgtaac aggtgttgtg ctgacaaccg tcctttttat catcccgttt aattcaagtt
     8101 ccttaagttc ccgcgacagc atacgggggg ttatcttcgg aatattctgc tggatctcgg
     8161 tgaatcgctt cttattgtaa agaagggatc cgattatggg caatttccac tttcctgtaa
     8221 ttacattcaa ggtatcattg attgccagta cacatctaac agggcatgcc ttgatctctt
     8281 caaaagtcag atttttatcc ataatggttt aaaccgatat ttgttttttt atgaataccg
     8341 gtatacaaaa gtatagtact tcttcagaat agcaaacata gtgtattttt gtacaaggta
     8401 taccctcatt tttaaagaat cgcctaaact taaagtcaat agaaatgaaa aagcgagaat
     8461 ttataaaaaa aggattcctg ggaattggaa gtttttttct ggccgggaag gcaaaagggc
     8521 ttgaatatta tcctatgcct tcagataaaa ggatagcagt catttacgct acatggtgcg
     8581 gtacttcacg tgatgcggct ttatggattt ccgagggtat ggatggtatc gccaatgtat
     8641 ttgatgtaag ggaggatcct gatatttcgg gatatgatca tcttatcata ggaggatcga
     8701 tcaggcagaa taaggtaccg gggcagcttg agaatttcct ggagaagaac agggctgccg
     8761 tttcaggaaa agtcagggga cttttcgccg tttgcggcaa tatgcgccag cctgtaggaa
     8821 atgaacaata caggaccttt atccaggatt atcttgcacc cctgtcggga gccgggcagg
     8881 ttccctcccg gattttcctt ggcaggatca cttgggggct gatggaacca gaagtatgtg
     8941 aaatgatgaa aaagatacct ggcatggaag agtatgacaa tctgaaaaga ccggaatgca
     9001 tggcgttcgg acgggagatc ctcgatgcaa tcaataaagt caattaatca ccataactaa
     9061 tactaaacca aacaatacaa aaaacaatca tgaaaaaact gctactatta ctgatcattg
     9121 tgtttccatt agtttctgtt tattcacaga tagatgataa gctgatatcg gatattaaaa
     9181 attcgggata tgttcaccgg ccgctgccac tggatcacag caagtcttat gaggcattcg
     9241 cgatcacgaa gaaagttctg gtctcggata tgctctgtga tatggaagac ttaagcaaat
     9301 ggactcacag cggggtggga ggcatgaggc tgacatcgga acgttccgta tcgggaaaac
     9361 acagtctcag gctcgtcgca cctgcagttc cggataaata tcccggctgg ggcataggaa
     9421 gaggatcgtc tgtggccagc tacgatgtgg gaggaaggaa tcttgagaaa tacaacaggg
     9481 taaggctcag ggttttcccc gattgtgaag gcgcaagatc catctatctg aacctccttt
     9541 ttgagaacga cggaaaggag aaggttcccg atccttttgg cagggaaggg atccatgaga
     9601 tgaaccttgt caatggccaa tggaacgatt gcgttgttga aatacccgaa ctggcccgcg
     9661 acaaggtcac aaagatatct ctggccattg agatcttcgg gagggagcag acaatgggcg
     9721 actcactgaa gtttgacata gacgccattg aactgcaggt tgttgaaaaa cctgaagtgg
     9781 tcagcggatg gatccccgca gaagacagga tcatcttctc aaccaccggt tatgggctaa
     9841 acagcgaaaa gacagcaatt gtaagaacaa gtgaaaatgt ttccaggttc cagcttgtcg
     9901 acaaagccac ctcaagggtt gcctatgaag gtaacatcac ccagacaaag acccggctgg
     9961 gagaattcaa gatccttaat ttcactgact tcagggatgt cggggagtat ttcctcaggg
    10021 tgggaaagat cgaaacacag ccgttcttta ttaacaagcg catatgggaa aattccatgt
    10081 ggagggtgct gaattttgtt ttctgcgagc gctgcggtta tccggtgccc gacaaacacg
    10141 gtgcatgtca tggcgatcat cacgccgtgt tcaatgacaa gatctttcct gttcacggcg
    10201 gctggcacga tgctgccgat atgtcgcagc agtcattgca aactgcagag gttgcctttt
    10261 cgctgttcga agtggcaaac aacgcaaaga ggaataataa tcacgacctt tacaacaggg
    10321 cacttgaaga agctgaatgg ggacttgatt atattcttaa aacgaggctt ggcggtggat
    10381 tcagggtaca gaactggggt accaatctgt ggaccgacgg ctacctgggt acagatgacg
    10441 acaaaggctg gcaccaggcc ggagttcacg acagcggttt tgaaaacttc cttttctcag
    10501 gcattgaggc ctatgcagca ttgagcatat cacacgatga aatgctgaag ggtcatctta
    10561 taaaggccgc aaccgaagat tttggttttg ccctgaaacg gtttgaagaa aaaggctatg
    10621 ccgaaatggt cgttgactgg ggcggtcatg catatatggc ctcggagagc cagtatatgg
    10681 catcggtttc atgggcggca agccagatct acaggcttac cggcgacaaa tactatgctg
    10741 acaaggctgc tgaattcatc cagtatgttc ttaaatgcca gagaaccgag ccattgaatg
    10801 acaaggacaa gctgagggga ttcttctacc gcgatcttgg caaaaaatca attgtgcatt
    10861 tcaaccacca gtcgcgcgat caggtattca tgcaggcact tactgaactc tgccttaccc
    10921 agcccggtca caaggattac ggcaaatggg aaaactcaat caggatctac ggggagtatc
    10981 tcaaaaaact catgagctat gttgcccctt acggaatgct ccccagcggc gtttatcaca
    11041 aggacgaggt aaaggacacg atcaattttt tcagatggca ggtcggaaga agcaaggaac
    11101 ccgagaagga ttacatggaa caacttaaca atggagtgaa actcgacaat gagcattacc
    11161 tgagagtatt tcccgtttgg ttctcattca agggtaacat tgcggttcat ctggcaactg
    11221 gaaaagcagc tgctttatgc ggtaaatttt tgaacgacaa ggaacttatc aatattgccg
    11281 aacagcagtt atgctgggtg gtgggcaaaa acccgttcgg ccagtccatg atctggggag
    11341 aaggcagcaa ttacgctcag caatatactg cactgcccgg tgaaaccgtg ggagaaatac
    11401 cggttgggat gcagtcgaag ttcaacggtg acattcccta ttggccaaat atcaatacgg
    11461 ccgtctataa ggaagtatgg tcttcatccg caggcaagtg gctctctctt gtcgctgaat
    11521 tctgagcacc ctgaacaccc ggacagcaag tcggaaaata ttctgtataa tgccggcagc
    11581 gaaataagga tcagcgtaaa tcacggaatg ctttcacgtt gaccttattt ctgccctggc
    11641 cggatatatc ttatgtttct ttatttgaat gagcatcgcc gggtgcactg tgctgacagt
    11701 acttttttcc cgccgcaggc gcggcagtga ccagcctgtt ttaaacttgt taattttgcg
    11761 cttctacatc atttggaatt tttatgacaa aacccattat actgctggca gtctcattat
    11821 tgatcatcac tacgaaaatt tttcccggtg aggcggtgaa tataaaaaga attaaaggcg
    11881 aaattctgtt cgacggcata cctgatgagc ccgaatgggc taatctgacc tatctcccca
    11941 tgaccatgca cagtccggtt tttggagctg aaccttctga agcaagcgaa gtgcgtatcg
    12001 gctatgacga tcatttcctg tggatcggag ccagtcttta tatgaatgat cccggtaaga
    12061 tctttgccgt cacaaggaaa cgcgacgaca ggcttttcgg ccatgatgca ttcggtatca
    12121 tcctcgatac ctacaacgat aaggagaatg ccctggtatt ttatacttcc cccaacggga
    12181 tcagaaccga tttcactatt tccaatgatg ccgtaatgaa cggcggcggg ttcggattga
    12241 attcctcatt taacataagc tgggatacct tctgggacgt gaaaaccaga attgacaata
    12301 agggttggca tgtggaaatg aagatccctt tttccagcct gaaattcaaa ccttccgggg
    12361 atgttacaac aatgggactt attgtgtgca ggaacattag cgccaacaat gagactgaca
    12421 cctatccggc aatagatcca aagtatggca tgattgcctc aaacaaaccc tctcttgctc
    12481 aggaaataat tattgaaggg gtaaaaccat caaaaccggt atatatttct ccttacctcc
    12541 tgggaggagg aacccgtgaa tacaatctca atgaactgca gaccggttat gtcaggaatg
    12601 aaaagcccga atataatgca ggtctggata ttaaatacaa catcaacagc aaccttacac
    12661 tcgacttaac cgcaaatacc gactttgcac aggtagaagc ggatgatcag caggttaatc
    12721 tgacacgtta ctcacttttc tttcctgaaa agaggaaatt tttccaggaa cgatccagcc
    12781 tctttgattt cagcctcgga ggattctctg acaatatgtt ctacagcagg agcatcggcg
    12841 tttttaacgg atcgccggtt agaatatacg gcggtgccag aataacggga agaatgggaa
    12901 gatgggatac gggattttta aatatgcaga ccggagccca tgattccgtt ccgggtgaaa
    12961 atttcggcgt cctcagaatg cgacggcagg ttattaacca gaatagctat gtgggaggaa
    13021 ttatcacctc aaggctgggg atgaacggtt acaaaaactt cggttacgga attgacggaa
    13081 tattccgcct gttcggcgtc gattacctga atgtaaaatg ggcacagaca tacgacagca
    13141 accttgaaaa caaaatgaat tcacttgatc cgtcattcat tcttgtcgac tgggaaagaa
    13201 gaaatgaaaa aggatttgcc tacaggttca actacaccta ttcaggtgaa caacttaaac
    13261 ccggtgtcgg attcgttatg agaggggcgg ttcagggatt gaacggccgg ctttcatatg
    13321 gatggttccc cggcgaaaaa tccaggattt acaactacag tgtaagcgtt atggcggaac
    13381 agtataaccg ccttgatgac gggaatcttg aatcggcaat gattacgccc ggtatcaata
    13441 tcgggacaaa aaaaggaata aatgccgggc tttcagctga agtccggaaa gaaggtgtca
    13501 ggaacgattt ctacctttct gaaaacgtcg ttgtaaaagc aaatgaatac acttttacca
    13561 atggacaaat gatgttttca accccaaact caaaaatgat atctgtaatg accggtatca
    13621 acgccggcca gttctatgat ggcctgagat acggtaccat gaccatggtc aatttcaaca
    13681 tttcctctgg ttttaacctg tcggtaaact atgatttcaa tgccatccgt tttcctgaca
    13741 gggccagcaa caattcactg aatattcacc tcgtcaatgc aaaggctctt gtgatgttca
    13801 gcaccaaact tacagcggct gtcatggtgc aatatgtcaa ttcttacgat aatctcctga
    13861 ccaatttcag gcttaggtat aatccaagag agggaaatga cctgtatctt gtcctgaatg
    13921 ataaccggcg gatctcagga tcaggttcag ttccggaata tcccaggttt ttcaaccaga
    13981 cagttatgat aaaatacata catacgttta ttctttgata acaggcttgc gattgcctgc
    14041 cagattcatc acgaaccggc aatgaaatcc cctttgccaa aggcagcagc cgcaggccgg
    14101 gttagcgaac tgcaaccgga tcacatgatg gggtaaactc ccggtgcagt gcgacagctg
    14161 agatcatttt aaataaaact attccgccag tatgcccggg ccttaaccgg gtatggcggg
    14221 cggctttggc atctgacccg tccgggaatg attgttgcaa tgatatttca tttaaaatca
    14281 gttcttaaac ataaaactta tatttgtgtc acaatccgtt tctcatgtcg gaggaaagag
    14341 atctgattca gagaatatcc aggggcgaca cagatgcttt ccgtgagctg tttgctgcct
    14401 gttaccggtc gctcttttcc tatgctttaa gccttgttat tgacagggaa actgcaaaag
    14461 atattatcca ggatgtcttc ttccgcatct gggaaaacag gaagaacttt gaaattcaca
    14521 cgtctctgaa ggcattcctg tttgccagtg tccgaaattc cgcaatagat catatcagga
    14581 agcagaagcg cttttcagga tatgaaaggg agctggtgga aatattgtcg ggccagacct
    14641 cctccggtaa tgctgcaccc gaacccctga actcaataat ttacaatgaa acatgtgata
    14701 ttgttgagtc ggcaattgag aaacttcccg aacagtgccg caggatattc attatgagca
    14761 gatttcatgg actgaaacat aagcagatag cggaaaaact tgatatctcc tccaggaccg
    14821 ttgaggcaca catatacgca gccctgaaat ttttaaggga aacggttaaa aaatcccttt
    14881 ccgactaagt gatctcactt ttataaacgt cttatcataa catttgaaca aaatggatcc
    14941 atctgtcaat attgagcata tatttcttaa tctgctggaa ggcggcgaaa tttctgctga
    15001 agaactgaaa attctgcatg cctggctgga gtccaatcct gatgaggcag aaaagtacgc
    15061 cgacttcagg gaaatatggg ctcttgcatc ggtgcctgag aatatggctg aaagcgagtg
    15121 ccgggaggga tacaggcagc ttgagaaacg ccttcgggcc aaaaggtggc ccgtcatacg
    15181 catggaaaag cagaggagac tcagggttcc ggtgctggcc gctgcagtca ttgcaggaat
    15241 ggttatagcc atcggcgcac tgcacttatg ggacagcagg atcatccgta aggagagatt
    15301 cgttgttgaa gtgcctgcag gatcaagatc cgtaacaata cttcccgacg gtacaaaggt
    15361 gaccctcaac ggggactcaa aaatttcata tgatcaggga tatggcagaa gcaaccgtaa
    15421 tgttgatctt tcaggcgaag gttattttga agtagtgcat aatgcgcggc ataccttctc
    15481 ggtttgtgcg gcgggagcaa tggttgccgc acacggaacc gtcttcaatg tgaaagcata
    15541 cccggaagat ccgtccgttg agacaaccct tgttaaagga tcggtcagca ttgaaattcc
    15601 cggtcacagg aaagaaagaa tgttcctaaa accatcggag cagtttacgt ggctcaaacc
    15661 ggttgtcgcg gatgattcgg acaagggccg ggtactcata tcaaagggaa tagatactga
    15721 aagatacaca tcatggatca gcggcagact cattattcag agtgaattgc tggcggacct
    15781 ttccctcagg ttggaaagaa aatatgatgt tgaaataagt tttgaggaac aggacctcaa
    15841 aaatcttcgt ttcaccggcg tacttgagga tgaaacaatc gagcaggtgc tggaagtact
    15901 aaagattact tcaaaccttg attttaagat ttcgggaaga gagatagttc ttttttcctg
    15961 cagaaaaacc ccctgacatt taactcaaaa tccatcccga atgaacctaa cctaccgtaa
    16021 aatactcaaa aactggcgca aggcagcttt tctggcatta ctgatgcttc cgggcctcaa
    16081 caatatgtgg ggacaggaaa caaggctgaa tctgccgtca accacaatga ccctggcaga
    16141 actttttaaa agtgttcagg ataaaagtga gatcaggttt ttctacaatg atgatcttat
    16201 taatcctgat cagagagtaa cactgtcatc aacaaagcta acggtagccg agatgctgga
    16261 tgagctgaaa cggcaaacca gcctgtcgtt cagacttatg ggaagcaata tgattgccgt
    16321 gactgccggg acccaggcca gggatataca gcttagtggc cgggttacgg attcatcgac
    16381 aggagaaccg attgccggtg taaccgttac actcagggga acgaatatcg gggtactgac
    16441 tgacatcaac ggcaaatatt cagtcagtgt ccccgggaat gttgaaattg ttgctttctc
    16501 atttataggc tataatactg ttgaaatccc ggttgcaggg cggtcggtga ttgatgtttc
    16561 aatggaagaa ggtattcagg ctctcgatga ggttgttgtg accgctctca atgtcaacag
    16621 gacgaaaagt tcgcttgggt attccatatc aacagtaaag ggcgctgaca tagaaaaatc
    16681 aggtaacaac aatatgataa acactcttgc aggcaaggtc gccgggctgc atatctcgag
    16741 aacggccggc ggcgttgacg gatcaagcag ggtgatactc aggggggtaa tgtcgatcac
    16801 aaaagacagc aggccgctgg tcattgttga cgggataccg gttgatgccg gacactccag
    16861 cggcggcagg tggggaggat catccggtgg cgatgccctt ggtgacatta accccgatga
    16921 cattgaaaat atcagcatcc tgaaaggtgc aggggctgct gcagcttacg gatcaagagg
    16981 cgccaacggt gtgatcctca tcacaacgaa aaagggcgcg gaatcgaaag gactgggaat
    17041 taccgtaaac tcatccattg ttgctgaaac ccccttgctg ctgccccgac ttcaaaacaa
    17101 atacggccac ggatcgtttg gaacataccc tgcatcggtc cctgatgacg gcttcccgtg
    17161 gggatggagc tggggccctg aaatgaaagg acaaatggtc cggggatatg ccggtgacat
    17221 ggttcccttt tccccgcagc ccggtaattt cagggatttc ttccgcaccg gatcaatgca
    17281 ggctaatact gttaccattg aaacgggcac tcccgaatca agcgtcaggg catccttctc
    17341 cacacagaac aacaagggta tagtgccgcg gaacaatctg acacggcaaa ccattaacct
    17401 caggggtttt tccaggtcaa gggatatact cgaactcgac ggtaagattg cctatatgca
    17461 ttcaaagatc accggccgcc cggaagttgc agaaggaccg ggaaaccctg cctattacct
    17521 ttcgcaggcc ccaagaaaca taagcaacga ttacctgagg aacaattatg aagatgacag
    17581 cggaaaagag ctgctctgga caaaggatgt ctataccgga aatccatact ggcagctata
    17641 taacaaggat aacgaggagg agaaacaccg tgtgcaggga gttttctccg gaaaaataat
    17701 cgttatgccc ggactggata ttactgcccg gtcgggactc gacatgacat acaggctgaa
    17761 tcacagcgat cttgcaaagg ggagtatttg ctcaggcaat gttaacggaa gtgtttacaa
    17821 ttcaatttcc aatttccttg aatggaacag cgatgttctg gcaaactatt caaaccgcct
    17881 gggtgaggat ttgaaatata cagtcaattt cgggggcaac tatcgctata attacagccg
    17941 cggtattggt cagtacggat caaaccttaa gatcaacaag ttttatgcaa tatcaaattg
    18001 ctggagctac agcaccaatg aatggttgag caaaaaggaa gtgttttccg tttacagtct
    18061 cggacaggta tcattcaggg aatggcttta ctttgatgtt accctgagaa acgactggtc
    18121 atccactctg ccgccaggca gcaactcata tttctatcat tctgaaaacc tcagcttttt
    18181 gttcacaaag gcacttaata cagataccag aatactttca tcagggaaat tgcgtgcttc
    18241 atttgccaaa gtgggcaatg ataccaatcc ttatgaaaca aatcagtatt tttatgtgtc
    18301 ccagtcgcaa ctgccatatc cgttcggtac cttcagcgac gtacttccga catatgatct
    18361 tcagcccgaa cttacacaca gctgggaagc aggtgtaaat ctcgaattcc tgaacagccg
    18421 cataattctc gatgttactg cttatacgac aaattcaaac aatcagataa taagagtaca
    18481 gctcccggtc tcgagcggct tcaacaggaa agttatgaat gctgccgacc tgaggaacag
    18541 gggtatagaa ctgcaagctg atttttcagt catcaggagc agaaagttca actgggatat
    18601 gaccctgaca tggtcaaaaa acaagacaat ggtgaaggag ctctacaaaa acgccagttc
    18661 atttctcctc gatgaaacat ggtttgccac aatacaggct gtgcccggag aagagtacgg
    18721 cctaatttat accagggatt ttaaacgcga tgcatttggc cgtgtacttg ttgatgacaa
    18781 tggttttgtt ctggccgggg aatacaaaag aatgggaaaa ataacacctg actggaccgg
    18841 cagtatatcg gggaaggttt catacgggaa cttttccctt gcagcacttg ttgatattcg
    18901 tatgggagga aacgtttatt caataggaaa agcctaccgg tgtcttttcg gaacttcggc
    18961 cgagaccctt accggccgtg aggaatggta ttcgacgcat gatcccgaat acctttattc
    19021 ggtcccttta ccgggaattg accccaacgg gtatattgag aagggattca atgagaatac
    19081 cggtcagcca aatgcggccc ccgttgatcc catgtacagg tggtacaata tctggtcgaa
    19141 ggagatagga acggaatggc tccttgatgc taccaacgca agactgcggg aaatatctct
    19201 gacctattcc gttcctgccg gaatactgaa aaactttcct gcaaaggggc tctctgtatc
    19261 tgtcatcgca aataatcttc tttgcatcta caatgctatg aaagatatag atcctgaatc
    19321 aggttattca agcggaaatg tcggaggagg atttgaacat tccgccttgc cgtcaacacg
    19381 cacttatggt ttcaatgtaa aaatcgattt ctgatccctt ttactgctac tttatgaaaa
    19441 gatctgtaaa aagttttgtc tgcccggtcc tgataacgct tctagtcctg ctcacggcat
    19501 gtgacaagcg cctggaggaa attaacaggg atccgctggg cctggacgac ctgtcggatg
    19561 aatacttctt taataccggt ctcaggatgg catttggtga ttacttttgg ctgagatcgt
    19621 tccagatgcg ctttgcatca caatattccc acgtctatgt gaccagcact gagatgagaa
    19681 atgctgacag ctatgaagac tttcattccc aggatgttta caaggaaatg tttcaggctg
    19741 catatacggg ccctgcagta tatatgaaca aagtggtaag catgactgca ggggggaaac
    19801 atgaaaacaa tgtcagaaat gcaatcgcaa gaatcgtgtc ggctgtcatt tttgccacca
    19861 taacagattg ttacggtgat gttccctatt cggagggtgc aaaaggcatg gatggaatac
    19921 tgtacccgaa atatgattcg caggaattca tttactctga tatgatggag cagctgaaaa
    19981 ctgccattga ggtccttaaa accgctgatc cttcaagggg atacgtaaat gccgatccgg
    20041 tttatgacaa tgacctttca aaatgggtaa gatttgcgaa ttcgctcagg cttcggcttg
    20101 caatgagatc aagatttgcc agcccggcaa aaagcgctgc gattataacc gaatgttttg
    20161 caaattcctt tattgagacc aatgaccaga actttgcctg gaagcaccag gacagcgaga
    20221 acagtgaatt gtacaatcca tggtttgatg ttcaccgtaa tgaggatttc aggatgagcg
    20281 agaagtttgt tgactggctc agttcaacaa acgatcccag gctggagatc cttgtaagcc
    20341 ctgttccggg aagaggatat gtcggtattc caaacgggct cagcagtctg caggttggca
    20401 actatccgtg ggcaaattat tccaaacctt ctcccgccct gtatgcaagg gatcttcccc
    20461 agaccctgct gtgtgcttca gaggtatggt ttctcagggc tgaggcagct cttttcggcc
    20521 tggctcctgg agatcctaac gaattttacc gtgaaggcat tatcaggaac ctggaattct
    20581 ggaatgcaga tcccgctgaa acaaatacat ttcttaccga tgaacccgag gcaaccctta
    20641 gcggaacaat cgaaaacatg ttcaggcaga tatccaccca gatgtggata gcattcgtcc
    20701 ctaatttcgt agaggcgtgg agcaacatcc gcaggacggg cttccctgtt atcccgcaaa
    20761 gaaccaatgc tgagatcttt tcacttggcg ttacaaacgg aatactgccg aaacgtttta
    20821 aatatgccac gaatgaatac ctgacaaacg gaataaatct tcaggcggcc atcgatcgtc
    20881 agggccctga taagattgac acaccagtct ggtgggatgt attggacaga tgaaaaatca
    20941 ttaagcaact taaaacccaa aaagtatcat gaaaacaaat tatttcttaa ttctgatgat
    21001 tacagcaatt tttgctgtaa tgggcgtatc atgcaaaaaa acacctgcgc caacggctga
    21061 gatctttgcc acaattgaca gttatacagt aacatttaat cctacggtgt ctgatgtaaa
    21121 gacctatgac tgggactttg gcgatggaga aaaaagtact gaggctgctc cggtccacac
    21181 atataagaaa tcggggacat atactgtcag gttaaccgtg aaaggcgacg gaggtgaaat
    21241 ttctgtcacc aaggacatta ccattgcagc atcggtagag gagatgctca caggggggcc
    21301 ttctgctgtg aacggaaaga catgggtgct aagtacctcc tatactgccg gacaggacgg
    21361 tggcggtccg gttcagaatg ccatgccgat aaccattccc agtgccgaaa atgtcctcga
    21421 tctattcggt ctgggcgagg aatatgacaa tgaatttaca ttcttttcag acggcagcta
    21481 caaaatgact cccaagaacg gtaaggtact tgcaggtgca gtgtatgggg ttgccactgg
    21541 aaccatttcc ggcgatcccg tatatgaaat cggaatgtgt gccgcaacgt tcgatccacc
    21601 ggcaacagca acctggtcac tcggaaccac tgattttatt gtcgacgcaa tcaccgatcc
    21661 ggatgagact aacgttccgc cggttcacgc agatgtcacc ttcaccggta aaacctgggt
    21721 caagctttca gccggagctt atttcggagt actcgacttt cctacaatcg acaggttcat
    21781 tatcaagtcc atcacacctt caaaaatgac tgttgcttta ttcctatgtg gttatggcta
    21841 cggtgatgat cctgaaatga tcatgcttcc gacaaacctt atccaactga cgtacgtacc
    21901 caaataacag ggacagttct tgtcatgtac aggttaatcc cggcgtaaaa gtccaggagt
    21961 tatttctgcg ccgggatatt cctgatccga accatcaata atgggttatg aaacaattga
    22021 tcagaacagt gatctgcctt gcactggcat ttaccgcatg taaacaggca aacctgccaa
    22081 aggaatatga gtttggcgta attgtaaaca acggtatcac cgtggacggg caaaaattta
    22141 ttgactcata tggcaggcag gtgatactca gcggcattaa caaggtaaac aaaaatccga
    22201 atgacaatta tctcgacagg gactcggctg aatcgtacag gcagatgaaa gaatggggat
    22261 tcaatgttat ccggctgggc gttatctggg atggcattga gccggaaccg ggaatctata
    22321 atgagcaata ccttgatgaa attgaaaaac gggttaactg ggcagcggaa aatgggctgt
    22381 atgtaatgct cgacatgcat caggatcttt acggcgtctc aatgagtgtg aaagatccat
    22441 actcgggcga cggagcaccg gaatgggcta ccattacaga cggattaccc catcataagg
    22501 gggagatatg gagcgattcg tacattataa gcccagcagt tcaaaaagct ttcgatcatt
    22561 tctgggccaa tactccagcc tccgacggta tcggcctcca ggatcattat gctcttatgt
    22621 ggaagcatat tgcggcaaga ttcgcgggga acagtgcagt tatcggttat gatatcatga
    22681 acgaaccatt caacggcagc cagggaatac ctgtacttcc tgcaattctc agggaatttg
    22741 ccggagttta tgccgaagct accggaaccg tgcaggatga agaacaggtt atgacaatgt
    22801 gggcttccga agaaaaccgg atgaaagcat tttcatatat cgaaaaggcc gatgtatatg
    22861 caagagtgat tgatgctgct accgaaatca atcagcagtt tgaaaggaat gaactccgtg
    22921 atttctatca gaaggtggga agcagcataa ggcaggttga cacgacccat atcctctttc
    22981 tcgagcatgg ctattttgcc aatacgggca tgaggagcgc acttgaacca gtcaggcacc
    23041 cggacggtac cattgatcct ctcgtcggtt atgctgcaca cggatatgat ctgctcaccg
    23101 acacaaagaa tgttggaagc cagggagctg aacgtatcag ttttatcttc agcaggatca
    23161 acgaaacagg taaaagaatg aattcacccg tgcttatcgg agaatggggc gcattcggag
    23221 gaaacgcacc cgggctggta gctgtggcaa ggaacatact gaaactcttc gaagagtatc
    23281 atttcagcaa tacattctgg acctatttca acaacattca gaattacagt tttttcacgg
    23341 gagccattat aagaccttat ccgcaattca tcagcggaac actctcttct tattcattca
    23401 gccacgagac cggcgatttt aatatgacct ggcaggaaga cccggcagtg aaagcaccca
    23461 cggtgatttt tataccatcg cttgacaggc tggttaagga aagtgtcaga gtatctcccg
    23521 ggaatgaccg tattatcatt gaaacaatta agggtacaac aaacggttac attgtaatat
    23581 caccgcttgg aggtgcccag gagagaaatt tcagactgaa tctttcctat taaggttatg
    23641 tatgtagcct gacacgtagt tgatatccgg gaagaaatta cggtttagga atatttttcg
    23701 gggctgcgta tcggatatcc gcaaaggctc tcaactcctt tacctggttc atcccctgaa
    23761 gcctaaagat catttatatc cgaacgattt atttatttca ttcagcagat agtgtttctg
    23821 gtcagcaaaa tattcccaga acatgatccc ggccagcttg tttttcagga tatattcgca
    23881 ctttatcctg gttgattcct catcatcgta ggtgatgaat gccttttcat cggcattgaa
    23941 caggtacggg gcctttgatt cagcgtccca gtacctggtg aacccttttt tattgacaac
    24001 gctgtccttc aggtaagaat aaccaccccc cagccatctc cagaccggta cgggtatctg
    24061 gtacaaacca ttgttgtcgg tggtctttac aaccctgccc ttcccgtaga aggctgtgcc
    24121 aaggacaatc tttgacggtt tgacgcccac agccatcatg ttcctta
//
LOCUS       DAOOYG010000030        23390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_1999_length_23390_cov_7.627041, whole genome shotgun sequence.
ACCESSION   DAOOYG010000030 DAOOYG010000000
VERSION     DAOOYG010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23390
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_23390_cov_7.627041"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..2005)
                     /locus_tag="PK288_04435"
     CDS             complement(11..2005)
                     /locus_tag="PK288_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPM18198.1"
                     /translation="MFGSVAFLIIIALSLLIFAVSEPLKYYFTLALLLAGVALSGSWS
                     IDILTGSAPAIELILFRPAHGPGFVLTLDSLSAFFVIVINITVLIGFFYARGYLAPYR
                     QTKQNTRFSIHFFSYFWLWLSMLMVVMLRDGLAFLVAWEIMALSSFFLVIFEAEDRKI
                     MKTGISYLIQMHVGMFFILIAFLLVYRSTGRLSFDALAEYFDGHHNLPLFLLFFVGFA
                     IKAGFVPMHTWLPEAHPAAPSHVSGIMSGVMIKMGIYGIARVLASMQSDLLATGIFIL
                     VISIMSGLGGIMMALVQSDMKKMLAYSTIENIGIIGIGLGLGVTGQALGSQSLALLGF
                     AGALLHVFNHSLFKSLLFFNAGSVYYATHTRNMEQMGGLMKKMPWTAMMFLTGSLAIC
                     GLPPFNGFISEYLIYMGMFYSLSDATLYISLLMIVAILALSLIGGLALFAFTRAFGIV
                     FLGEARTAAMNHAGETTPGMIIPQVFTVGLILLVGLGSSVVVKPVFNNVMITWGLKGM
                     PMVAGALSYNLSRISLAGGILVVTLGALLLYRRYHLSRRVVEAGPTWGCGYTAITPRH
                     QYTANSFSGNFNVLAKPVLRTGKIVDLPDENELFPGKREFFTRSHDIFRLYLIDLPVN
                     RLSGLLKKIAVMQTGQIRHYILYAFLFMLLVLLLSLFNII"
     gene            2227..3138
                     /locus_tag="PK288_04440"
     CDS             2227..3138
                     /locus_tag="PK288_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM18199.1"
                     /translation="MNERKKVAWAYAGLTLMTLIIGFSFIFVKIALRHAPPSDLLAHR
                     FTTATIGLIIYYVFNRRKRPGIKRENIPALLALSLFYPLLLFGFQTLGLKYTSASEAG
                     IISAVTPIITLVLAALFLKEKNTPVQILFVLLSVSGVIYIMYRNGITAITGASMKGNM
                     LILLSVISIAIYYVMGRKANKQFNSMDITFFMSLTACIVFNCAAVAMHVKSGNLAHYF
                     DAFKNPGFLWPILYLGILSTFLTSFLGNNALSVIPAAQVSIFNNFSPVISVFGGVFFL
                     NETLKGWHIIGGILVLGGIIGVNYFRK"
     gene            complement(3344..4015)
                     /locus_tag="PK288_04445"
     CDS             complement(3344..4015)
                     /locus_tag="PK288_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18200.1"
                     /translation="MAGSSKGGVNQAKADDGNPKLVDEITETIMTSMKLGIGDVWVSQ
                     GVVREGEPDNLEWIKLTKDTNYELKLFEDYTFPAVELPAGEYKSIKIKFRNIFYRHCK
                     LISDPNVVYELLETMGSSTDPCDPADTSWAPDDYFSTGGNFSLVDGKFKLGSAKEKVG
                     GFTIKSGKTAKILWRLGGGVVATCVNKLIDINGNLEWDCDIDDIDIECTPEVEYMWDF
                     VVQED"
     gene            4809..6362
                     /locus_tag="PK288_04450"
     CDS             4809..6362
                     /locus_tag="PK288_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPM18201.1"
                     /translation="MKKVIILITLILIPAVLGSQPLKTADIDRLVIRTMETFNVPGIA
                     VAVIKDDNVVHMKGYGVSSIITGKKTDENTLFAIASNSKAFTAATLGILVDEGKIRWE
                     TRVTDIIPEFRLYNSYVTEDFNIKDLLTHRSGLGLGAGDLMLWPDSSLFTREEIIHNL
                     RYLKQVSPFRTKYDYDNLLYIVAGEIVARISGQSWEQFVEQRIMKPLGMDRSSASLKR
                     AKDMSNIIDAHVPVDGKLQVVPKYQGETLNSAGGIISSVADMSRWVIMLLNNGKYGEG
                     NTEQLFSEAVQREMWTPQTILPLRSSGPYRSHFAAYGLGWQLTDVAGYMQVSHTGGLN
                     GIVTQVTLIPELKLGIIVFTNQQEGAAFSAISNTIKDAYLGLKGSDWIKTLRDNTEWS
                     KTRAQRIIDEVWAKVEAQSSGNGIRPDLSSYAGTYLDSWLGEIMITEENGKLRFRSAK
                     SPRLRGDMYYYTGNTCIVKWDDRYMDADAYVVFSLDKDGKAAAFTMEAISPLTDFSYD
                     FHDLNPVRK"
     gene            complement(6374..7462)
                     /locus_tag="PK288_04455"
     CDS             complement(6374..7462)
                     /locus_tag="PK288_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18202.1"
                     /translation="MRITFLIIVFLHGLIHILGFVKGSGISEVKALKLPVSSLEGVIW
                     LVAALLIMVFGTLKSTNYRYAWLAGFIAVAVSQTLVIMFWEDARAGTLPNIMILLVSI
                     VSLGSFNFNKKVEEETRSLLNRVKDITPGIMHEGDIAELPEPVKQWLHRTGAIGKPFI
                     ISGRVVQRAQMKMKPGQKRWIRASALQYSTISVPGFIWTVNAEMNPLVYFMGRDKYFE
                     GQGEMLIKLNSLFNVANEKGPRLTESTVQRYLGEMVWFPSMAPGKNITWEQVDDVTVR
                     AEMNYKGVTAGGTFHFSKEGDFIRYSAQRFMENSPDAQRFEWVLDVEEYGTFGGIKVP
                     SRMTATWKLPDGDWTWLLLEIEDIKYNP"
     gene            7719..9146
                     /locus_tag="PK288_04460"
     CDS             7719..9146
                     /locus_tag="PK288_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM18203.1"
                     /translation="MNTKEIVDKLHELDQSSSELEIQESDRAALIKLVTDYSNEFIAG
                     LNDRNVFFERRPGSLEIGGNKKTMSELLDIYRKEVAETGINAASGKHLGYIPGGGIFA
                     AALADFIAAFTNPYAGVYYASPGAAGIENEVIRWLKKIFSFPESSVGNLTSGGSVSAL
                     IAFTAARDKYQVKNESVRKSVVYITEQAHHSARKALRIIGLEDVIIRYVPMDDHHRMK
                     TGELDELVQRDKTAGLNPFLVIGSAGTTDTGAVDPLDGIADIAGKHNLWFHADAAYGG
                     FFILTSKRELFMGIERADSIIVDPHKGMFIPYGVGAVLIRDKASVLYSHQYAANYMQD
                     AAAEELIQSPSNLSPELTKHFRGLRVWLPLQIHGIEPFISCLEEKLLLVKYFRNRLGE
                     LGFRLGPEPDLSVSYFWYPFETDQDEKNRQLMKEIHADGDVFLSSSVIDGRFVIRIAI
                     LAFRTKKASIDRALEMIKRCLEKIR"
     gene            complement(9229..9660)
                     /locus_tag="PK288_04465"
     CDS             complement(9229..9660)
                     /locus_tag="PK288_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18204.1"
                     /translation="MAGFEGNLKPSVPRKVLMLIAGAMWCGVGLMLSLMAYRWLSDYE
                     GRTWLFAIPGLTAAMIIHHFGFLKIVDKNLDRISRLPDQSCAFAFISWKSYLIIVIMV
                     SMGIGLRHSSIPKPYLSIIYLGIGVALFLSGTRYFRKLLTG"
     gene            9970..11367
                     /locus_tag="PK288_04470"
     CDS             9970..11367
                     /locus_tag="PK288_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPM18205.1"
                     /translation="MGRLRLSGQELKRIGYKSDRTISLAINLARKNFKKAGREEILIT
                     LEKINLQPHKYLKDPVWGKLAQLLTEKPEEGRKVPDLKKSRRYFKIFGEELIDPEAIR
                     QMELAMSLPISVKGALMPDAHVGYGLPIGGVLAAYNAVIPYGVGMDIGCRMCLSVFPF
                     SPRKINDDREQIKRILMNETRFGLAEFRDEQDHEILGRKEFDEIKFLRTLHKKFAAQL
                     GTSGHGNHFVDVGHVIIPEYSELIKLQAGEYFAVLSHSGSRNFGAEVCRHYTGIARGK
                     LGLRGAEAQLAWLELGSEEGEEYWAAMNLAGDYSHANHRIIHRRLARAFGEAPLVIIE
                     NHHNFAWKETLATGEEVVIHRKGATPAGIGDIGVIPGTMASPAFIVGGKGNEESLNSA
                     SHGAGRKMSRNVAVKDLSGKQMKEYLDSRRIELLGAGTDESPFAYKDIHEVMDYQKDL
                     VEILGTFHPAIVRMA"
     gene            complement(11574..12017)
                     /locus_tag="PK288_04475"
     CDS             complement(11574..12017)
                     /locus_tag="PK288_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18206.1"
                     /translation="MKRNLLLTVVALLAFLTGSCTKDDIKLSEFVVGTWKSQEINLGG
                     PTKIDMKVTLIIVINENKTYVLSMKFSEGGDLVSLPQAEYVLDESRNQITIKQPEFEP
                     VKNSEDSITFEVEWEKGSKKMTWTPLENPEAPTLIWTKQKGPITE"
     gene            complement(12301..13962)
                     /locus_tag="PK288_04480"
     CDS             complement(12301..13962)
                     /locus_tag="PK288_04480"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPM18207.1"
                     /translation="MKRNFPKVLFFLLLGLICTGPATFSQLRGLGTISKKELKYNLDF
                     LGAVEFRGRETPSPELEIATLYLGNWAGRTGLKPVMKDGSFYQPVPVTVASVFQPGTR
                     ITLTGANGDRIYYFGKELNGNFNRSGTYRGEVVFAGLGISDPETGWDDLKNLDLRGKI
                     VVILDAQRPGTVFPLGFTMTGRLMSRIFELRNRGAAAVLSVVSPERERRKAGGQNIFD
                     YIPEGRMTPVYETQRINLGQAAAVQAAEEKTRPSLPFERAEISHDLAADMLGITKDEI
                     AGMFSKIEQGLQVPSFAVSGISARLDVEVETFRSESRNVLAMVEGSDPVLKNEYIVIS
                     GHHDARGIDDGAIIAGADDNGSACVALMEIGRALLVERPKRSVILAWFTGEEQGMNGS
                     HYFVNNCPVPVEKISVCLNMDMISRNDPDSLYLVGSDLLSSELDASIRKVNKKYGINF
                     TFDYLYSNLTHPQRVYFRSDQYPFVRLGIPSVWFFCGFTYDYHTEWDIIERVDYDKLY
                     KTTRLVYLTAYDIGNMKHLVRLDVNPAVTSRGKHNLPEKSLFGGR"
     gene            14541..15521
                     /locus_tag="PK288_04485"
     CDS             14541..15521
                     /locus_tag="PK288_04485"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM18208.1"
                     /translation="MKRKLLFIGLLTALLVIGLDSCKKESEAKEPPTCDFSIKAEGLK
                     ITFTAVATNTDTYAWNFGDGQTSTEANPVHTYADGGEYEVKLVVTGEGGSAEKKIKVT
                     VTPGLEDIKIMLTGGPGAANGKTWVLKTDSPVDGDGASAIEPNMLVLIPIPADFYGWM
                     GKERLDGKKEEYTFKYDGSFKIDTKNDTVLAISLYAFFNGITVDQSNTPYGTCRATGY
                     VQPAGATWEIRESDITVDAITNPSETTVPPAHGNVVFSGKKWLSLSPGSFLGFLDFST
                     SSNIIIKSISPTQLRVAIMVCMYQGAAAPGGADFANLPTYMYHMTFIPKP"
     gene            15647..17293
                     /locus_tag="PK288_04490"
     CDS             15647..17293
                     /locus_tag="PK288_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPM18209.1"
                     /translation="MKKHNRFLKTAAVILIITGVSVLLLINRFTSRRTETEITMSDGT
                     PLSATIFIPAGRGKYPSVLVRTPYNKQAEEWVGKAFNIFGIAVITQDVRGKFKSGGDF
                     YPFINERKDGLETIRWIRKQPWSDGTVAGWGASYLGYTQWAVSDSLDFMTLMLTGARL
                     FDLVYPDSLFSLQTAGIWGFSSASRTVNDLNEDTVRKKLYTLPVMAADDSAIRDINYY
                     NDWVIHDKYDSYWEQMDFRGRSDAPVLSVAGWYDLFLKCQLADFLALDSSGNNRGRLV
                     IGPWAHGEPGEASQYGGPEKTGDPKLIFKYVRQYLKGRKARPGSPMKDAKYNLFIMER
                     NEYAGSDVWPPRETTMTPYHLGPGGYLGPAIPSEKGSLSYEYDPADPYPSLGGTALGK
                     GVGPARQNPNTGRKDQLVFRKKIESEPLILLGPLGATLWLSSSAVCTDYYVLLQDEFP
                     DGKIINIQEGGAKVKLTGNGPEKTEISIWATGYQLNPGHTLRVVITSSWFPRFNRNLN
                     RCEKAADAKITMRSTQTLWYGNGTPSSVSLPVYLPGNPGY"
     gene            complement(17326..19335)
                     /locus_tag="PK288_04495"
     CDS             complement(17326..19335)
                     /locus_tag="PK288_04495"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPM18210.1"
                     /translation="MITQDTLFEEMTDISRLSSAKGMNYRVVQYSSYDRRSVSPLDST
                     WFANEDGFGGEPIPGFEKVLAAPDSSGTGTYLICDARGPGVVQRLWTAGISGTVRVFL
                     DSPDNLLFQGKAEDFFRKPVEILSGDSSLGKHTESFYQYDASYFPIPFSKGFRMEWTG
                     KISDIHFYHVGLRLYDNDQEVESFTRENFRKYKATMIRTDSLLRNPDHRPASGHYSSM
                     NGEVRLEPGEQAVLMDLKGPSSITRFSLRADAGNLENALRQAVLRIWFDDSETAQVNA
                     PLGDFFGAAPGINPYISLPFTVLTDGTMICRFEMPFSKNARIIIENHSAEKIKVSAEA
                     IVARFRWKDGESMHFHALWSMDHDLTTSELDSRASDIPCLAAEGKGRIAGAAALIHNP
                     SGVPTSWGNWWGEGDEKIFIDRDTFPSFFGTGSEDYFNYSWSSSRIFSYPYCGQPRND
                     GPGNRGYVSDFRWHISDDIPFRESACFLMELKHHGKVEGFSYGRIIYYYLLPGGNTVT
                     GLFTAHDLREINYEPWMPEAFKGSDGWRFVQAEELARGNDRVSFEKDDFWAGSGVLNW
                     IPASGQDVLKLNIPSDKEIRETRIGITLTHTPDSGSLTFRLNGEPVRFSGRDTLLVFT
                     AAGRLLDNHFSGPVRLKKGSNELMIGMPGADGRKTAQIDFIWLRK"
     gene            complement(19419..21077)
                     /locus_tag="PK288_04500"
     CDS             complement(19419..21077)
                     /locus_tag="PK288_04500"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPM18211.1"
                     /translation="MVRIAIILLLLILSGCTKNTVTLGKLLSEMISAESASYFPYPEY
                     RLLQASSYNRKSVSPDSAGWFENNDMSHFIRIEENQGRREFVMLDTDGPGAIVRWWMT
                     FYKAQEGIIRIYLDNSEDPVIAGAPDDLLSGSLLAPYPFSASVQEGAPLGEEGRDYDY
                     NLYLPVPFSKHCKITYECDSLRILYDYEGTNVPEGYYWPDVFYNINYRLYDKTVSVTS
                     FTMKELGRCRKKIRDAGTILLSEAPLSGRKVSGEMRLLPGDSLVLSDHSGPSAVGSLR
                     ARIISSCPDQSLRSVVLSIRTDGIASVWVPAGDFFGSGYTSQTHRTLFNSRDSSGMMS
                     SFRIMPYRKNISISFVNYGKDTVDVRVMADLIPYRWNDRSMYFGASWHEYHRLRTRDA
                     SGSPFDLTFIKLTGKGVYVGDQLTLFNDTYHWWGEGDEKTFVDNEPFPSIFGTGSEDY
                     YGYSFGRREAFSHPFLSQPVGIGNMARGVTINMRHRALDAIPFRKSIDSRIEMWHWAD
                     IEMNIALTSYYYFFDPVIRNIKPDIPSVRKKVAVHRHEPDDENE"
     gene            complement(21071..22831)
                     /locus_tag="PK288_04505"
     CDS             complement(21071..22831)
                     /locus_tag="PK288_04505"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM18212.1"
                     /translation="MKEMHQKIFTLLILAGLLVSCSEDYLNRTPLDEISPGDYFRTAN
                     DLKLYANRFYPLLPSHSGYGGGTFWLDTNSDNLVPGTEDMRLAGTRTVPSSGGGWNWE
                     EIRQVNYFLDHCFQYPGEEDLKKIYIAEVRLFKAMIYFDKLKTFGDVPWFSVALNPDS
                     PELFSGRLSREIVTDSIIACIDYAIANLKPRSHSEEFRLNREVAILFKARVCLYEGCW
                     EKYHKGTPFGVQGKDGTKFLQMAADAADLLMQAGTSNLYRGPARQEYFSLFNKLDYSG
                     IPEVLLWKKYDIGLGVYHWCNQYLPFGAGNLGVSRSLMDDYLCTDGLPIAVSPLFRGY
                     DSIQAESADRDPRLAQSMLLPGDDVTINSPGGSLDKKWWMPALDQSQQFRATTGYCMN
                     KGANLDYSQQLSSGGTMASIFFRYTEALLIYAEARAELGTITQDDIDKTINKIRDRVG
                     MIHLDISNITVDPDWDFPELSPIINEVRRERRIELAFEAQRWDDLARWRAHRVFAGKR
                     PKGIKYIGSNLEGKYKDYLGKPTIVIGQNLFVDSDGRIDPYQVKFPNGLGFNPERDYL
                     SPIPSDELTLNKNLVQNPGW"
     gene            complement(22875..>23390)
                     /locus_tag="PK288_04510"
     CDS             complement(22875..>23390)
                     /locus_tag="PK288_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family protein"
                     /protein_id="HPM18213.1"
                     /translation="DKSIIGNSTPRFAYSLTLNAGWKGLDLNIIWQGIGKRDLWLDSP
                     IFWGAGGIYWLTAYEGHMDYWTPENTDAFWPRPYMDHGFKNKQVQSRYLQTGAFLRLK
                     SLQLGYTIPYKLTKKFYITNLRIYTSGENLLTFTKLFEAFDPEATDGERAHGYIYPLQ
                     KIISGGLSITF"
BASE COUNT         6354 a         5635 c         5335 g         6066 t
ORIGIN      
        1 cctgtcattt tcagataata ttgaataacg aaagcaacag tacaagaagc atgaacagga
       61 aagcatataa tatgtaatgc ctgatctgcc ctgtctgcat caccgctatc tttttcagca
      121 atcccgagag ccggttcacg ggcaagtcga taagatagag cctgaaaatg tcatgactcc
      181 gggtaaagaa ttcacgtttg ccggggaaca attcattttc atcgggtaaa tcaacaatct
      241 ttcctgtacg cagcacgggt tttgcaagca cgttgaaatt ccccgaaaac gagttagccg
      301 tgtattgatg ccgaggtgtt atcgcggtgt agccgcaacc ccaggttgga ccagcctcga
      361 cgactctcct gctcaaatga taccgacggt acaggagcaa tgcacccagg gtgaccacaa
      421 gaatgccacc ggcaaggctg atccgcgaca aattatagga taaagcaccg gcaaccattg
      481 gcataccttt caggccccat gttatcataa cgttgttgaa caccggtttt actacgacag
      541 aggaacccaa gccgaccaaa agtatcaatc ccactgtaaa tacctgggga ataatcatac
      601 ccggcgtcgt ctctcccgcg tggttcattg ctgcagtcct ggcttcaccg aggaaaacaa
      661 tgccaaacgc ccttgtaaag gcaaaaagcg ccaggcctcc aatgagtgac agggcaagaa
      721 ttgccacgat cataagcagt gaaatatata gggtggcgtc ggaaaggctg taaaacatac
      781 ccatgtaaat caggtattcc gaaatgaacc cgttgaaggg aggcagtccg caaatagcca
      841 gagacccggt taaaaacatc atggcagtcc acggcatctt cttcatcagc cctcccattt
      901 gctccatgtt cctggtgtgg gtggcataat aaaccgatcc ggcattgaaa aacagaagcg
      961 acttgaagag cgagtggtta aaaacgtgca gtaaagcacc ggcgaatcca agcagggcca
     1021 gcgattgact tccaagggcc tgtcctgtca ctccaagtcc aaggcctatg ccaataatac
     1081 cgatattttc tatggtgctg tatgccagca tcttcttcat gtcgctctga accagggcca
     1141 tcatgattcc tccaagcccc gacatgattg atattaccag aatgaatatc cccgtcgcca
     1201 ggaggtcaga ctgcatcgag gccaacaccc gagctatacc ataaataccc atttttatca
     1261 tcacacccga cattattcca gagacatgcg acggggccgc cggatgagcc tccggcagcc
     1321 aggtatgcat gggaacgaaa cctgccttga tggcaaaccc aacaaagaaa agcaggaaca
     1381 acggcaggtt gtgatggccg tcaaaatatt ctgccagggc atcaaaactc aaccgcccgg
     1441 tagatctgta aacaagaagg aaagctatca aaatgaaaaa catcccgaca tgcatctgga
     1501 tgagataact gataccggtt ttcatgatct tcctgtcttc cgcctcaaaa atcacgagga
     1561 aaaaggatga taaagccatg atttcccatg caaccaggaa ggcaagtccg tcgcgcagca
     1621 tgacaaccat cagcatcgag agccaaagcc agaagtagga aaaaaaatga atcgagaaac
     1681 gagtattttg ctttgtctgc cggtaagggg ccagataacc ccgggcataa aaaaatccga
     1741 tcagaactgt tatattaata acaatgacga agaaagcact caggctgtca agggtcaaaa
     1801 caaaaccagg gccatgagcg ggccggaaaa ggatcaattc aatggcagga gccgatcctg
     1861 taaggatatc aatagaccat gagccggaaa gagccacgcc ggccagcagc agggcaaggg
     1921 tgaaataata cttgagaggc tcagacactg caaatatcaa cagagacagg gcaatgatga
     1981 ttaggaatgc aacggaacca aacatcggaa atacatttga ccgcgaatgt ataaaatatc
     2041 agattcaaaa atcaatcctg attcccggac gtgagtgata aataatgatt cggggttgtt
     2101 tcacctggtt catcctggga ccggcaaaat atcttgccat ggtgcctgaa tgatgaatat
     2161 cagtaccccg gcaccaggat agttacataa acttatgcta tttttaccgg ttactataag
     2221 gaccggatga atgaaaggaa aaaagtagcc tgggcttacg cgggccttac actgatgaca
     2281 cttattatcg ggttctcctt catttttgtc aagattgccc tcaggcatgc cccgccttct
     2341 gaccttctgg cgcatcgctt caccactgcc actatcggat taattatcta ttatgtcttt
     2401 aacaggagaa aacggccagg gataaaaagg gaaaatattc ctgccctgct ggccttatca
     2461 ctgttctacc ccttgctgtt gttcggattt caaacccttg ggctcaaata cacgtcagcc
     2521 tctgaggccg ggatcatctc ggctgttaca ccaatcataa ccctggttct tgccgccctg
     2581 ttcctgaaag aaaaaaatac cccggtgcaa atccttttcg ttttattgtc cgtatcgggg
     2641 gtaatctata tcatgtacag gaacgggata accgcgataa ccggagcaag catgaaaggc
     2701 aacatgctga ttctcctgtc ggttatctcg atagccatat attatgtcat ggggagaaaa
     2761 gccaataaac agttcaattc catggacatc acctttttca tgagtctcac ggcatgcatc
     2821 gtgttcaact gcgccgcggt cgccatgcat gtaaaatcag gaaaccttgc ccactatttc
     2881 gacgcgttta aaaacccggg atttctttgg cccatcctct atctcgggat tctttccacc
     2941 ttcctgacgt ccttccttgg caacaatgca ctgtcggtga taccggctgc gcaggtgtca
     3001 atattcaata atttcagccc ggtgatttca gtgtttggcg gggtgttctt cctcaatgaa
     3061 acacttaagg gatggcatat cataggtgga atactggtac tgggaggaat aataggcgtt
     3121 aactatttcc ggaagtgaaa aaaaccggct tccatacccg actattcctc cccgcggtac
     3181 ccgtatttta gtgttaatca attaacaaat gagcttttcg ccttattcct gctacgctcc
     3241 gattaacagg acagatacta atttcaaccc tcacggcgtc ctgcttgcat gttacttaca
     3301 ggaatacaaa taattcttgt ttaaatactt ttaaggttgc tgtctagtct tcctgaacaa
     3361 caaaatccca catgtactcg acttctggcg tacattcaat atctatgtca tctatatcgc
     3421 aatcccattc aaggttgcca ttaatgtcaa tcagtttatt gacgcacgtt gctacaactc
     3481 ctccgccaag cctccataag attttggcag tcttaccgga tttgatcgta aatccgccca
     3541 ctttttcctt tgctgagcca agcttgaatt tcccatctac taaggaaaag ttacccccgg
     3601 tgctgaaata gtcatccggt gcccatgagg tgtcagccgg atcgcaggga tcggttgaac
     3661 tgcccatcgt ttccagtaat tcatacacaa cgttgggatc agatattaat ttacagtgcc
     3721 tgtaaaagat gtttctaaac tttattttga tgcttttata ctcaccggct ggtagttcaa
     3781 ctgccggaaa ggtataatcc tcgaaaagtt tcagctcgta attggtatcc ttagtcagct
     3841 ttatccactc caggttgtcg ggttcgcctt cccttacaac cccctgtgaa acccatacat
     3901 ctccaatccc cagtttcatg cttgtcataa tggtttcggt tatttcatca accagtttag
     3961 gattaccatc atcagctttt gcctgattca caccaccctt ggaagatcct gccatgggat
     4021 taatacattt aacttccagg aaaccagtgc ctgtaaggtc atcatcattc cctttttcct
     4081 agctgcagcc cgcaaaaata agtgaagcag taaccaaaat tccaaataat ctcattgttt
     4141 tcatcatcat tctttttagt ttggaaatat ttgttaattc aaaatatcat acgtattact
     4201 caaaaacctg gtcattccaa ttcagatcct gcctgacagc aagaaaaaac cttccctgca
     4261 atcgcgcatg attgctttcc gaagtagttt cttaaatgct ggtgccggtc ctggccaggg
     4321 ccgttgattg cctgaattgc aacatccagt taagttaacg cccgctgaat aattaaattg
     4381 ccctgacccg gatcttcatt accaaaaatc tgcatgaatc ctgatatata tctgacaacc
     4441 agggattcca gatttcggaa tcagacggat tcaaatttaa attaatggta caaaatgacc
     4501 ggttcatcgt aaatatgcag gacctggtac tgtctatttc tgtttttttt atttatgcgt
     4561 catgattgtt tgaagtcccc tcctgctgaa tcgataatta gtaaaaaaat taagtctggc
     4621 aacataattt catagaaaaa ttcctgcata acccgttttc aaactgtttt ataaaatcct
     4681 tagtctgatg agaataatat ctttgatatc aattactaaa agcaggggaa atattgaaaa
     4741 tgaaaaacag agttacattt accataattc tccggaatca taatctcact ttgaaaattt
     4801 tcaaccagat gaaaaaagta atcatactaa taacgctgat cctgatccct gcagtcctgg
     4861 ggtcgcagcc cctgaaaacc gcggatatcg acaggcttgt aatcaggacg atggaaacct
     4921 tcaatgttcc cgggatcgcg gttgcggtga taaaggatga caatgttgtt cacatgaagg
     4981 ggtacggggt cagttccatc attaccggta aaaaaaccga cgagaacacc ctctttgcaa
     5041 ttgcatcaaa cagcaaggca tttacagcag caactctcgg gatccttgtt gacgagggta
     5101 aaataaggtg ggaaaccagg gttactgata tcatacccga attcaggctt tacaactcct
     5161 atgtcacaga agattttaat ataaaggacc tcctcacaca tcgcagcggg ctgggcctgg
     5221 gagcgggcga cctgatgctc tggcccgact cctccctgtt caccagggag gaaattatcc
     5281 ataacctgag ataccttaaa caggtatccc ctttccgcac taaatatgac tatgacaacc
     5341 tgctttacat agtggccggc gagattgttg caaggatctc cggacagagc tgggaacagt
     5401 ttgttgagca acgtatcatg aagcctcttg gaatggacag gtcatcggca tcattgaaaa
     5461 gagccaaaga catgtcaaac atcattgatg cccacgtgcc tgtagatggg aaactgcagg
     5521 ttgtacccaa ataccaggga gaaaccctta actccgcggg cgggatcata agcagcgttg
     5581 ccgatatgag ccggtgggta ataatgcttt taaataacgg caaatacggt gaaggcaata
     5641 ctgaacagct tttctcggaa gccgttcagc gcgagatgtg gacacctcag actatactcc
     5701 ccctcaggag cagcggaccc taccgcagtc attttgctgc ctacgggctc gggtggcagc
     5761 tgacagatgt tgcaggatac atgcaggttt cccacacggg cggattaaac gggattgtta
     5821 cgcaggtaac acttatcccc gagctgaaac tgggcatcat tgtctttaca aaccagcagg
     5881 aaggagctgc attctcggcc attagcaata ccataaagga tgcttacctg ggactaaaag
     5941 gcagcgactg gatcaaaacc ctccgggata atactgagtg gagcaaaacc agggcccaaa
     6001 gaataatcga tgaggtatgg gcaaaagtcg aggctcaaag ctcgggtaat ggcatccggc
     6061 cggatctctc ttcctatgcc ggcacgtacc tcgacagctg gcttggcgaa atcatgatca
     6121 cggaagaaaa cgggaaactc aggttcagat ctgccaaatc acccaggctc aggggcgaca
     6181 tgtattatta taccggcaac acctgcattg tgaaatggga tgacaggtac atggatgcag
     6241 acgcttatgt cgttttctca ctcgacaagg acgggaaggc tgcagccttt acaatggaag
     6301 ccatctcgcc cctgaccgat ttcagctatg atttccacga cctgaatccg gtacggaaat
     6361 aacagtcata aattcacgga ttgtatttta tatcttcaat ctcaagcaag agccaggtcc
     6421 agtcaccatc cggaagtttc cacgttgccg tcatcctcga cgggaccttt atcccaccga
     6481 aggtgccgta ctcctcaacg tcgagaaccc attcgaatcg ttgggcatca gggctattct
     6541 ccatgaaacg ctgcgcagaa tacctgatga agtcgccttc cttactgaaa tgaaatgttc
     6601 cgcctgccgt taccccctta tagttcattt ctgccctgac cgttacgtca tcaacctgct
     6661 cccaggtaat gttcttaccg ggagccatgg agggaaacca gaccatttca ccaagataac
     6721 gctgcactgt tgattcagta agtcttggcc ccttctcatt tgccacgttg aataacgagt
     6781 ttaatttaat caacatttct ccctgccctt caaaatattt atcccttccc atgaagtaaa
     6841 cgagaggatt catttcagca ttaacagtcc agataaagcc cggaaccgaa atagttgaat
     6901 attgcagcgc tgaagccctt atccaccttt tctgaccagg cttcattttc atctgcgccc
     6961 gttgaacgac cctgccactg atgataaagg gcttcccgat tgcccccgtg cgatggagcc
     7021 attgttttac cggttcaggc agttccgcga tatcgccctc atgcatgatg ccaggggtaa
     7081 tatcttttac tctgttcaac aaacttcgag tttcttcttc aaccttttta ttaaaattaa
     7141 aactgcccag agagacaatt gaaaccagaa ggatcataat attgggaaga gttccggccc
     7201 gggcatcctc ccagaacatg ataaccagtg tttgcgatac tgcaaccgca ataaaaccag
     7261 cgagccaggc atacctgtaa ttcgttgatt ttaatgtgcc gaaaaccatg atcagcaacg
     7321 ctgccaccag ccagataact ccctcaaggc tggaaaccgg cagttttaac gctttaacct
     7381 cgctgatccc tgatccctta acaaatccca ggatatggat aagtccgtgc aggaacacaa
     7441 ttatcaaaaa cgtgattctc attcaactca agcagcctgt gtttttctcc gtaattgatt
     7501 ttttcctgat ctcaactttg ctggtacatt aacacataag tgccctaatt tacaataaat
     7561 atggggaatc agtatcctgc aggaaaaatc gttgtcagaa taagaaaaac acttctccca
     7621 cgagaacctc tttattgcaa aatcgcctgg cgcagttgaa cagcccgtaa aaaataaaat
     7681 aactttgccg gaaatttaac ttttttacgg gagatcttat gaacacgaaa gagatcgtgg
     7741 ataaactcca tgagctggat caatcgagca gtgaacttga aatacaggaa tctgacaggg
     7801 cggcgctgat taagctggta acggattatt caaatgaatt tattgcaggg ttgaatgaca
     7861 ggaatgtatt ttttgaaagg cggccaggat cccttgaaat cggcggcaat aaaaaaacca
     7921 tgtcagaact tcttgatatt tatcggaaag aagtggcgga aacaggtatc aatgctgcat
     7981 cgggcaaaca tctgggatat atcccgggcg gcgggatctt tgccgcagct cttgccgatt
     8041 ttattgcagc ttttaccaat ccgtatgcag gtgtgtacta tgcttcaccc ggtgcagccg
     8101 ggatagaaaa tgaagttatc cgctggctga agaaaatatt ttcttttcct gagagttcgg
     8161 tgggcaacct gacatcaggc ggatcagtat cggcgctgat cgcttttacg gccgcaaggg
     8221 acaaatacca ggtaaagaac gaatctgtcc gtaagagcgt ggtttatatc acggagcagg
     8281 cgcaccattc tgcccggaaa gctttgcgga tcataggcct cgaagacgtg attatccgtt
     8341 atgtgccaat ggatgatcac cacaggatga agaccggtga actggatgaa ctggtgcaaa
     8401 gggataaaac tgccggactt aatccttttc tggttatcgg gtcggcggga acgactgata
     8461 ccggagctgt tgatcccctg gacgggatag ctgatatcgc ggggaaacac aacctctggt
     8521 ttcatgccga tgcagcttat ggcggattct tcatcctgac atcaaaaagg gaattgttca
     8581 tgggaattga aagggccgat tcgatcatcg ttgatcctca caagggcatg ttcattccct
     8641 acggagttgg tgcagttctg atcagggaca aggccagtgt tttgtattcg caccagtatg
     8701 ctgcaaacta catgcaggat gccgctgcgg aagagctgat tcaaagcccg tcaaatctct
     8761 cccctgaact gacgaaacat ttcaggggtc tccgcgtatg gctgcccctt cagatacatg
     8821 gtattgaacc tttcatatca tgcctcgagg aaaagctgct attggtaaaa tatttcagaa
     8881 acaggcttgg tgaactggga ttcagactcg ggcccgagcc cgacctgtca gtaagctatt
     8941 tctggtatcc gtttgaaact gaccaggatg aaaaaaacag gcagctgatg aaagaaattc
     9001 atgccgacgg agatgttttt ttaagctctt ccgtaattga cggcagattt gtgatccgga
     9061 ttgccatact ggcattcaga acaaaaaagg catccatcga ccgtgccctg gaaatgataa
     9121 aaaggtgcct cgaaaaaatc cgttaaaggg gctgcgccat gttaacaacc agggatcacc
     9181 gggcccttgc ctggaaattt tgttataccc tgattacggt cctggttttt aacctgttaa
     9241 cagtttccgg aaatacctgg tgcctgaaag gaacagggcc acacctatgc caaggtaaat
     9301 tatcgagagg tatggcttgg ggattgatga gtggcgtaaa ccgatcccca ttgaaaccat
     9361 gatgacaatg ataaggtagc ttttccagga gataaatgca aatgcacatg attgatcagg
     9421 taatcgtgaa atacggtcga ggtttttgtc gactattttc aggaacccga aatgatgaat
     9481 gatcatggcg gcagttaatc cgggtattgc aaaaagccag gttcttcctt cgtaatccga
     9541 taaccaccgg tatgccatga gcgataacat taatcccaca ccgcaccaca ttgccccggc
     9601 aataagcatt aataccttcc gcggtacgga aggtttcaga tttccttcaa atccggccat
     9661 gcaatctcag gctttccagg tcaaatatga tttataatct tcaattaagc aagagaccag
     9721 gggtgcaatc gttacacaag agctgcgcct gttataataa tcaccttctg tacaactggt
     9781 tacaggaatt taagccataa cagacttttg catatactcc ttcatatttt cacatggcac
     9841 tcatgctgca tggtgcaagc atatgtcata tcaggaacag agcagttcgc cgattgacaa
     9901 taaaagctta attttaacat atcataatat ccgaaaggtt tttgccgccc ggataccgga
     9961 aatcctgcca tgggtagatt aagattaagc ggacaggaac ttaaacgcat cggttacaaa
    10021 agtgaccgta ccatctccct tgccattaac ctggcgagaa aaaactttaa aaaagcgggc
    10081 agggaggaaa tccttattac cctggaaaag atcaacctgc aacctcataa gtatttgaag
    10141 gatccggtct ggggtaaact ggcacagctt ttaactgaga agcccgaaga aggcagaaag
    10201 gttcctgatc tgaaaaaatc caggcgttat tttaaaattt tcggtgaaga actgatcgat
    10261 cctgaagcca tccggcagat ggagctggca atgagtctgc ccatctcggt aaaaggtgcc
    10321 ctgatgcctg atgcccatgt gggatacggc cttccgattg gcggtgtgct agccgcatac
    10381 aatgcagtaa ttccgtatgg ggttggaatg gacattggct gcaggatgtg tctttctgta
    10441 tttccatttt ctccccggaa gataaatgac gacagggagc agataaagag gatactgatg
    10501 aatgagaccc gtttcggact ggccgaattc agggatgaac aggatcatga aattctcggg
    10561 agaaaagagt ttgatgagat aaaattccta agaacccttc ataaaaagtt tgcagcccag
    10621 ctgggcacat ccggtcatgg aaaccatttt gtggatgtag gtcatgtaat tatcccggaa
    10681 tattctgaac tgataaagct tcaggcgggt gagtattttg ccgttctgag ccattccggt
    10741 tcacgcaatt tcggagccga agtatgcagg cattataccg gtattgcccg cgggaagctg
    10801 gggttaaggg gcgcagaagc acaactggca tggctcgagc ttggctctga agaaggagag
    10861 gaatactggg cagcaatgaa tctggctgga gattattcgc atgcaaacca tcgcatcatt
    10921 catcgccgcc tggccagggc tttcggtgaa gcgccgctgg tcatcattga gaatcatcac
    10981 aattttgcct ggaaggagac ccttgccacg ggtgaagagg ttgtcataca caggaagggg
    11041 gccactcctg ccggtattgg cgatatcgga gtgattccgg gtaccatggc ttcccctgca
    11101 tttattgtag gtggcaaagg aaatgaggaa tcattgaatt cagcctccca tggcgccggg
    11161 cggaaaatgt cacgtaacgt ggctgttaag gatctgtcgg gtaaacaaat gaaggaatat
    11221 ctcgatagca ggagaataga gctcctgggt gcaggcaccg atgaatcgcc atttgcatac
    11281 aaggatattc atgaggtgat ggattaccag aaagacctgg ttgagatcct tggtactttc
    11341 catccggcaa tcgtaagaat ggcctagtcc cagggatccg caatattcag ttcaccttaa
    11401 tctcagtgtt cgtcagtaaa atcattgacc actgaatggt atatggctaa tctgtgggta
    11461 ttggaagcat gcaacagttt tgactgaaaa ctgatccaaa cgcttccctt aataacatat
    11521 aaaataaatg aggcggcact cagaatgaaa cctgaacaca agttacgaat atcttattct
    11581 gtaattggtc ctttttgctt tgtccagatg agagtgggag cctccggatt ctccaatgga
    11641 gtccacgtca ttttcttgct tcccttttcc cattcaactt caaaagtaat cgagtcttca
    11701 gaatttttaa ccggctcgaa ctcaggttgt ttaatggtga tctggttcct ggattcatca
    11761 agcacatact ccgcttgagg caaggaaacg aggtctcccc cctctgaaaa cttcatgctg
    11821 agaacgtagg ttttgttttc gttgataaca attattaagg ttaccttcat atcaatcttg
    11881 gtaggcccgc ccagattaat ctcctgtgat ttccacgttc ctacaacaaa ttcagacagt
    11941 ttaatgtcat ctttggtgca actaccggtc aaaaatgcca gcaaagccac aactgttaaa
    12001 agcagatttc ttttcatgtc aatactttgt tgtttttatg ataaaacccg taaaacagat
    12061 gggtattata ttatttacga aaaatattaa tgatatgtta cgcaaaggct tctgaataat
    12121 tatctactga tcgggatgga ttcttttttt ctgaaccggg gaagtttcca tgcagttttt
    12181 atgagcggta ctggcacggc tttccataca aaggaactga caatgatatt taactctgcc
    12241 agatacggca gaaagttatt catattgaat gcggctccaa tttgtgagcc ggaacgggat
    12301 ctaacgtccg ccaaacagac ttttttccgg caggttatgt tttccgcgtg atgtgacggc
    12361 aggattcaca tcaagcctga caagatgttt catattgccg atatcataag ctgtaagata
    12421 tacaagcctg gttgttttat aaagcttgtc ataatcaacc cgttcaataa tatcccactc
    12481 agtatggtag tcatacgtaa agccgcagaa gaaccacacc gacggaatgc ccagtctgac
    12541 aaacggatac tggtcgcttc tgaaatagac tctctgcggg tgagtcagat ttgagtacag
    12601 gtaatcgaaa gtgaaattta tcccgtattt cttattcact tttcttatcg atgcatccag
    12661 ctccgagctc agcaggtcgg agcctacaag gtaaaggctg tctggatcat ttcgggagat
    12721 catgtccata ttaaggcaca cactgatctt ttcgaccggt accggacagt tgttaacgaa
    12781 atagtgtgac ccgttcatcc cctgttcctc accggtaaac caggcaagga tgaccgatct
    12841 tttaggccgc tcaacaagca gggcccggcc tatttccatc agtgcaacgc aggcgcttcc
    12901 attgtcgtcg gctccggcaa taatggcgcc atcgtcgata ccccgtgcat catgatggcc
    12961 gctgatcaca atgtattcat tcttcagcac cggatccgaa ccttccacca tggctagcac
    13021 gttacgagat tccgaccgga aggtttctac ttccacatca agccgggcac tgatgccgga
    13081 aacagcgaac gacggcacct gcagtccctg ctcaatcttg ctgaacatcc ctgcaatttc
    13141 atctttggtg attcccagca tatcagcagc cagatcatgg cttatctcag ccctttcaaa
    13201 cggtaaggac ggccttgtct tctcttccgc tgcctgcacg gcagccgcct ggcccaaatt
    13261 tatcctttgg gtctcataaa ccggggtcat cctgccttcc ggtatgtagt cgaaaatatt
    13321 ctgccctccg gctttccttc tctctctctc cggactcaca actgacagga cagctgcagc
    13381 ccccctgttg cgcaattcga aaatcctgga catcagcctg cctgtcatgg tgaatcccag
    13441 cgggaacaca gtacccggac gctgggcgtc aagtatgaca actatcttac ctctgagatc
    13501 caggttcttc agatcgtccc atccggtttc cggatcgctt attcccagtc cggcaaagac
    13561 cacttcaccc ctgtaagtgc cgctccggtt aaaattgcca ttcagctcct tgccgaaata
    13621 ataaattctg tcgccattcg ccccggtaag agtgatccgg gttcccggct gaaaaaccga
    13681 agctactgtc acgggaacag gctggtaaaa tgagccgtct ttcattaccg gtttaagccc
    13741 tgtacggccg gcccagttgc cgagatagag agtggctatc tccagctccg gcgaaggcgt
    13801 ttcccttccc ctgaattcaa cagcgccgag aaaatcgaga ttatacttca gttccttctt
    13861 tgaaatggtc cccaggcccc taagttgtga aaaggttgct ggcccagtac atatgaggcc
    13921 cagcaagagg aagaacaaaa cttttggaaa atttcttttc atgatacatg tctggtttaa
    13981 gttcaggagc atcagaaaat gctttcaggc tactaatgat gccatatgta aagataaata
    14041 attcctgggc cttgcctgat aattgtaagt taccgtttaa taatttcctg aaatatttag
    14101 ctacggggga agaaaatccg gcaatcttac attcgtacga ggacatacag tatcagttgt
    14161 tcattgctgt ctttcacagt cagaaccagg aaaaaccggt cagttgttcc ccatttcgat
    14221 acttttaacg tttaagcatt aatttttcat ggggcggaat actgttttat cagtgcactc
    14281 atgccggttt atctgcatat cctcccggca ataagccaaa catcggcatc agcggcgaat
    14341 ctgaaatcca taatgatttg taccctgagt ctatggaagt taaaatctga taattgtttg
    14401 ttgtaatcat aaactgatac cagttttctg cttccttctc ctgtgccggg ctcaggcaga
    14461 caggaaaaaa gtgataataa atttgtttat ttaaacgttt aagcgtaatt ttgtttcaag
    14521 aattaaacca acaatctgaa atgaaaagga aattactgtt tattggcttg ctgacagctc
    14581 ttcttgtaat cggactggac tcctgcaaga aagaatctga ggcaaaagaa cctccaacat
    14641 gtgatttttc aatcaaggcg gagggcctga aaattacatt caccgctgtt gcaacaaaca
    14701 ctgacactta tgcctggaat ttcggcgacg gccagacaag tacggaagca aatccggtgc
    14761 atacctatgc cgacggcggc gaatatgaag ttaagctggt tgtaaccggg gaaggcggat
    14821 cagccgagaa gaagataaag gtaacagtca cccctggtct tgaagacatc aaaataatgc
    14881 tgaccggtgg gcctggagct gcaaacggca agacctgggt gctgaagaca gacagccctg
    14941 tcgacggtga cggagccagc gccattgagc ccaacatgct tgttctcata cctataccgg
    15001 ctgatttcta cggatggatg ggcaaggaaa gacttgatgg caaaaaggag gaatatacct
    15061 tcaagtatga cgggtcattc aaaattgaca cgaagaatga tacggttctg gcaatttccc
    15121 tgtatgcctt tttcaatggg atcaccgtgg atcagagcaa cactccctat ggaacatgca
    15181 gggccaccgg ttatgtacag cctgcaggag ccacctggga gatcagggaa tcagatatta
    15241 ctgtcgatgc aataacaaat ccttccgaaa ccacagtacc ccctgcacat gggaatgtgg
    15301 ttttttcagg gaagaaatgg ctctcccttt cgcccggttc gtttctgggg ttcctcgact
    15361 tttcaacttc cagtaacatt attatcaaat caatttcccc gacccagctg agagtggcca
    15421 taatggtctg catgtatcag ggtgcagcgg cacccggagg cgctgacttc gcaaatctgc
    15481 cgacatacat gtaccacatg acattcatac cgaaaccgta atgtctgctt atgcattgtc
    15541 attgtacggc ccggaaaccg caaccacagg tcaggctctc cgggccgctt tttttgattt
    15601 acccgtctgt gttaaacttc tcccattgcg ttaaacacct cctataatga agaagcataa
    15661 caggttcctt aagactgcgg cagtaattct tatcatcacg ggagtgagtg tgctgctgct
    15721 gatcaacagg tttacaagcc ggagaacgga aaccgaaata accatgagcg acggaacacc
    15781 gctcagtgca actatattca tcccggccgg cagagggaaa tatccttcag tgctggtccg
    15841 cacaccttac aacaaacagg ctgaagagtg ggtgggaaag gctttcaata ttttcggtat
    15901 tgcagtaata acccaggatg tcaggggaaa attcaaatcc ggaggtgatt tctatccatt
    15961 catcaatgaa agaaaagacg gtcttgaaac catccgatgg ataagaaagc agccatggtc
    16021 cgacggaaca gtggcaggct ggggtgcaag ctatttagga tacacccagt gggcggtttc
    16081 tgattctctt gattttatga ctcttatgct gacaggagcc aggttgtttg atttggttta
    16141 tcctgattcg cttttttcgc tgcaaactgc cggaatatgg ggctttagca gtgcttcacg
    16201 caccgttaat gacctcaatg aggacactgt aaggaaaaag ctatatactc tgccggttat
    16261 ggcagcagat gattctgcaa tcagggacat caactactac aatgactggg ttattcatga
    16321 taaatacgac agttattggg agcagatgga tttcaggggc agatcggatg ctcccgttct
    16381 ttcagtggcc ggatggtatg atttgttcct taaatgccag ctggcggatt tcctggcgct
    16441 tgattcttcc ggtaataacc ggggcaggct tgtcataggg ccgtgggccc atggtgaacc
    16501 gggggaagcc agtcaatacg gcggaccaga aaaaacgggt gacccgaaac tgatttttaa
    16561 atatgtacgg cagtatctta aaggcaggaa agccagaccg ggaagtccca tgaaagatgc
    16621 aaaatacaat ctgttcatca tggaaaggaa cgaatatgcg ggctcagatg tctggccacc
    16681 cagggaaacc acgatgactc cctatcacct cggacctggc ggatatctcg ggcctgccat
    16741 cccttccgaa aagggaagtc tgtcatacga atacgatccg gccgatccat atcccagtct
    16801 cggcggcacc gccctcggca aaggagtggg accagcccgg caaaatccga ataccggtcg
    16861 gaaggaccag ctggttttca ggaaaaaaat tgaaagcgaa cctctcatcc ttctgggtcc
    16921 tcttggcgca actctctggc tttcctcatc tgcggtatgc actgattatt atgtgctgct
    16981 gcaggatgaa ttcccggatg gaaagatcat aaacatccag gaaggagggg caaaagttaa
    17041 attgaccggg aatggtcctg aaaaaacaga aatttctata tgggccactg gttatcaact
    17101 caatcccggg cataccctga gggttgtgat cacctcatcc tggttcccaa ggttcaacag
    17161 gaacctcaac cgctgtgaga aagcggccga tgccaaaatc acaatgcgtt cgacacaaac
    17221 actctggtat ggaaacggaa caccttcatc agtcagtctt ccggtttacc ttcctggcaa
    17281 tccagggtat taactgttat cgggtaattt ccggatttcc ggaatttatt ttctcagcca
    17341 gataaaatca atttgtgccg ttttcctgcc atcagcgcct ggcatgccga tcatcaattc
    17401 atttgatcct tttttcagcc tgaccggacc ggaaaaatga ttatcgagca gcctgccggc
    17461 cgccgtgaaa actagtaggg tgtcacggcc gctgaatcga accggctccc cgttcaacct
    17521 gaaggtcagt gaaccggaat caggggtatg ggtaagggtt ataccaatgc gtgtttccct
    17581 gatctccttg tcggaaggga tattcagttt cagtacatcc tgcccgctgg caggaatcca
    17641 gttcaggaca ccggagccgg cccagaaatc atctttttca aaactgaccc tgtcattgcc
    17701 gcgcgcaagt tcttcagcct gaacaaacct ccatccgtct gagcccttga aagcttccgg
    17761 catccagggc tcataattta tttctctcag gtcatgtgca gtaaataacc ctgtaacagt
    17821 attgcctccg ggcagcaggt aatagtaaat gatgcgtccg tatgagaatc cctcaacctt
    17881 tccatggtgt ttgagttcca taagaaagca ggcgctttcc ctgaaaggga tgtcatcact
    17941 gatatgccac ctgaaatccg aaacatatcc cctgttgccc ggaccatcat tccttggctg
    18001 tccgcaatag ggatacgaga atatccgtga agatgaccag gaataattaa aataatcctc
    18061 cgatccggtt ccaaaaaagg aaggaaaggt atccctgtca atgaatattt tctcatcacc
    18121 ctctccccac caattccccc acgaggtggg aaccccgctg ggattatgga taagtgctgc
    18181 agctcctgca atccttcctt ttccttcggc ggccaggcag ggaatgtctg acgcccggga
    18241 atcaagctct gaagttgtca gatcatgatc catgctccac aaggcatgga aatgcataga
    18301 ttctccgtcc ttccatctga acctggcaac aattgcctca gctgacacct taattttttc
    18361 agctgaatga ttctcaatga tgattcttgc gttcttgctg aagggcattt caaaccggca
    18421 gatcatggtt ccgtcagtaa ggacagtgaa tggcagggat atatagggat taattccggg
    18481 tgctgccccg aaaaaatcac ctagcggagc attcacctgt gcagtctcag aatcatcgaa
    18541 ccagatcctc agcacggcct gtctcagggc attctcaagg tttccggcat cggccctcag
    18601 actgaaccgg gtaatcgagg atggtccttt cagatccatg agtactgcct gctctccggg
    18661 ctcaagtctg acttctccat tcatggagga gtaatgccct gaagcaggcc tgtgatcagg
    18721 gttcctcagc agtgagtccg ttctgatcat cgttgccttg tacttcctga aattttccct
    18781 ggtgaaggat tccacttcct ggtcattgtc atacaggcgc aatcctacat gatagaagtg
    18841 aatgtcgctg atcttaccgg tccattccat ccggaatcct tttgagaaag gtatcgggaa
    18901 ataagatgca tcatactggt aaaatgactc agtgtgtttt ccgaggctgc tgtcacccga
    18961 cagtatctca accggtttcc tgaaaaaatc ctctgctttt ccctggaaaa gcagattgtc
    19021 aggagaatcc aggaaaactc tcacagtgcc tgatattcca gctgtccaaa gtctttggac
    19081 cacacccgga ccccttgcgt cacatatcag gtatgtcccg gtaccagatg agtcaggggc
    19141 ggcaagcacc ttttcaaatc cgggtatcgg ttctcccccg aaaccatcct cattggcaaa
    19201 ccaggtgctg tcaagtggac tgacgcttct gcgatcgtat gatgaatact ggacaaccct
    19261 gtagttcatc ccttttgcag acgacagtct tgaaatatca gtcatttctt cgaacagggt
    19321 atcctgggta atcattttgt ggtcccctgc gcaagaaatt aatgcaacga tggtacaaaa
    19381 tgcaggcagc aggaatttaa gcggattctt catattcttc actcattttc atcatcgggt
    19441 tcgtgtctgt gtaccgctac ttttttccgc actgaaggta tgtcgggttt aatatttctg
    19501 atcaccggat cgaaaaagta gtagtacgat gtgagggcaa tgttcatttc aatatcggcc
    19561 cagtgccaca tttcaatccg tgaatcgatt gattttctga atggaattgc atccagtgcc
    19621 ctgtgtctca tgtttattgt tacccctctg gccatattcc cgataccgac cggctgagaa
    19681 aggaagggat gtgagaaggc ttcccggcgt ccgaatgaat atccgtaata atcctctgaa
    19741 cctgtgccaa atattgacgg gaaaggttcg ttgtcgacaa aagttttctc atcgccttct
    19801 ccccaccagt gataggtatc attgaaaagg gtaagctggt cgcctacgta tacgcccttc
    19861 ccagtgagtt ttatgaatgt aaggtcgaaa ggggagccgg acgcgtcgcg tgtgcgcagg
    19921 cggtgatact catgccatga ggccccgaaa tacatgctcc ggtcattcca cctgtatggg
    19981 atcagatcgg ccataaccct aacatcaacc gtatcctttc cgtaattgac aaacgagatg
    20041 gaaatgtttt ttctgtaggg cattatccgg aaagagctca tcatcccgga ggaatccctg
    20101 ctgttgaaga gagtacggtg ggtctgggaa gtatatccgg aaccgaagaa atcgcctgcc
    20161 ggaacccata ctgaagcaat tccgtcggtt cttatgctta acaccaccga tctcagggac
    20221 tggtccggac aggatgaaat gatccttgcc ctaaggctgc ctacagccga agggcctgaa
    20281 tgatctgata acacgagtga gtcgccaggt aaaagcctca tctctcctga aacctttctg
    20341 ccggataagg gggcttctga taggaggatt gtccctgcat ccctgatttt ttttctgcac
    20401 cttccgagct ctttcatggt aaatgaggtc acgctgaccg ttttatcata tagccggtag
    20461 tttatgttat aaaatacatc gggccagtaa tatccctccg gcacgtttgt tccctcataa
    20521 tcatagagga tcctgagaga atcacactca tatgttattt tgcaatgttt tgaaaaagga
    20581 acagggagat acaggttata atcataatcg cggccttctt ctccgagcgg agcgccttcc
    20641 tgcactgagg cagaaaatgg gtaaggcgca aggagtgacc cgctcagcaa atcatccgga
    20701 gctcccgcaa ttacaggatc ctctgaatta tcgagataaa tgcggattat gccctcctgc
    20761 gccttgtaaa aagtcatcca ccatcttaca atcgcaccgg ggccgtcagt gtcgagcata
    20821 acaaattccc tgcggccctg gttttcctcg atccgtatga aatgagacat gtcattgttc
    20881 tcaaaccatc cggcgctgtc gggcgaaact gatttcctgt tgtaactgct ggcctgcagg
    20941 agcctgtatt ccgggtaagg aaaataagac gccgattcgg ctgaaatcat ctcagaaaga
    21001 agtttcccaa gggttactgt attttttgtg caccctgaaa gaataagcag cagaagtatt
    21061 attgcaatcc ttaccatccc ggattctgaa caaggttttt attaagagtc agttcatcgc
    21121 ttggtatcgg ggaaagataa tccctttcgg gattaaatcc taaaccattc gggaatttaa
    21181 cctggtatgg gtcaatccgg ccgtcagaat ccacaaaaag gttctgtccg atgactattg
    21241 taggtttgcc cagataatcc ttatattttc cttccaggtt tgatcctatg tacttaattc
    21301 cctttgggcg tttgcctgca aacactctgt gagccctcca ccgggccagg tcatcccacc
    21361 tctgggcttc aaatgccagt tctatccttc tttcccttct tacctcattt attatgggag
    21421 agagttccgg gaaatcccag tcaggatcaa ctgtgatatt actgatgtcg aggtgtatca
    21481 ttccgacacg gtcacggatc ttgttaatgg ttttatctat atcgtcctgt gttattgttc
    21541 ccagttcggc cctggcctca gcatatataa gtaaggcttc tgtataacgg aaaaaaatac
    21601 ttgccatggt tcctcccgag ctcagctgct gggaataatc gaggtttgcc cctttgttca
    21661 tgcaatagcc ggtggtggcc cggaactgct gactctgatc gagagcgggc atccaccatt
    21721 tcttgtcgag cgatcctccg ggggagttaa ttgtaacatc atcgcctggc aaaagcattg
    21781 actgggcaag acgcggatcc ctgtcagccg attccgcctg aattgaatca tatcccctga
    21841 acagggggct gactgcaatt ggtaagccgt cagtgcacag gtagtcgtcc ataagactcc
    21901 gggacactcc gaggtttcct gccccgaatg gcagatactg attgcaccag tgatatacgc
    21961 ccaggccaat gtcatatttt ttccagagaa gtacttccgg gatccctgaa taatcaagct
    22021 tattaaaaag agaaaaatac tcctgcctcg ccggccccct gtagagattt gaggtgcctg
    22081 cctgcatcaa aaggtcagct gcatctgccg ccatctggag gaatttcgtt ccgtcctttc
    22141 cctgcacacc gaaaggagtg cctttgtgat acttttccca gcaaccctcg taaaggcaga
    22201 ctcttgcctt gaacaggatc gcaacttccc tgttaagccg gaattcttcc gaatggctcc
    22261 ttggtttcag gtttgcaatg gcgtaatcaa tacaggcaat gatcgaatct gtaactattt
    22321 ccctgctcag tcgtcccgaa aacaattccg gtgaatcggg gttgagagcc acggaaaacc
    22381 acggaacatc tccgaaggtt ttgagcttat cgaaatagat cattgcctta aacaacctga
    22441 cttcagcaat atatatcttc ttaagatctt cctccccggg atactggaaa cagtgatcga
    22501 ggaaataatt tacctgcctg atttcttccc agttccagcc gcctcccgag gaagggactg
    22561 ttcttgttcc tgcgaggcgc atatcttccg ttcccggaac gagattatcg ctgttggtgt
    22621 cgagccagaa tgttcctccg ccatatcctg aatgagacgg taaaagagga taaaacctgt
    22681 tggcatagag cttgaggtca ttagccgttc tgaaataatc gccaggtgaa atctcatcga
    22741 gaggtgtccg gttgagatag tcttccgaac aggaaaccag aagacctgcc agtataagta
    22801 atgtaaagat tttttggtgc atctctttca ttgttgaatg atggttggaa ttcatcttta
    22861 atgtcggtat gatatcagaa tgttatgctt aaaccacccg agattatctt ctgcagggga
    22921 taaatatatc catgtgctct ttcgccatcg gttgcctcgg gatcaaatgc ctcaaacagt
    22981 tttgtaaaag tgagcaggtt ttcacccgag gtgtatattc ttaaattggt tatgtagaat
    23041 ttcttcgtaa gcttatacgg gattgtgtat cccagctgaa gggatttcaa tctcagaaag
    23101 gcccccgtct ggaggtacct gctctgaacc tgcttgtttt taaacccgtg gtccatatag
    23161 ggtctgggcc agaaggcatc agtgttttcg ggggtccagt aatccatgtg tccttcataa
    23221 gccgtgagcc agtaaatacc tccggcgccc cagaatatcg ggctgtcaag ccacagatcg
    23281 cgcttgccta tcccctgcca tataatgtta aggtcaaggc ctttccaccc ggcgttaagg
    23341 gtaaggctgt acgcaaatct aggagttgaa ttgcctatta ttgatttgtc
//
LOCUS       DAOOYG010000031        22878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2081_length_22878_cov_7.346142, whole genome shotgun sequence.
ACCESSION   DAOOYG010000031 DAOOYG010000000
VERSION     DAOOYG010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22878
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2081_length_22878_cov_7.346142"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2390)
                     /locus_tag="PK288_04515"
     CDS             complement(<1..2390)
                     /locus_tag="PK288_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HPM18214.1"
                     /translation="MKADFIVRFFCLLTAAVINAMLFNLHGQTPEEIEAKRVSLPNGW
                     HLTPVGKLIPLGDLPLNIAVSPDRKLAAVTNNGQSTQTIQLINIKKGSSCYETEIGKS
                     WLGLAFSDDGKYLYASGGNDNFIIRYSVKRNKLVNPDTIKTGEPWPEKISIAGIAPDD
                     SRNRLYAVTKENNSLYVIDTRLRSVISVHPLGGEGYTCILSPDRRTLYASCWGCDKII
                     VYDTFADRITGSVPVGDNPNDMCISHNGHYLYVANANDNSVSVIDTRQMKVIETLNTA
                     LYPESPAGSTTNSVALDEDGNTLFIANADNNCLAVFDVSQPGESRGKGFIPTAWYPTC
                     VRVVNDKILVSNGKGVTSMANPYGPNPARKDTEVIYQAGGKVQAVEVQYIGGLFKGTM
                     SLISIPDEKQMKIFTRAVYNNTPYSKENELLAADTPEGNPIPLKVGDPSPIKYIFYVI
                     KENRTYDQVLGDMPEGNGDPSLVLFGENITPNQHALAREFVLLDNFYVNGEVSADGHS
                     WTMGAYATDYLEKNWPTSYGGRGGGYPGEGAREIANNKIYLWDQCKKYGVSYRTYGEF
                     IGNDREPTIPVLKDHFCRNFTPWIQTIRDTVRFREWKQDFDSLLAINAVPQFNTIRFI
                     NDHTMGLSLNMPTPFAQVADNDLATGLFVDYLSHSPIWKETLIIIVEDDAQNGPDHVD
                     AHRSTAYIAGGYVKQGFVDHTPYTTASLLKTMELILGLPPMTQYDAAATPRWRCMNSS
                     PDHPPFKVKSCLVDLDEVNLAENPWQKASEQLDFTAEDRINDFLFNEIIWRAVKGL"
     gene            complement(2380..3285)
                     /locus_tag="PK288_04520"
     CDS             complement(2380..3285)
                     /locus_tag="PK288_04520"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPM18215.1"
                     /translation="MMDHQQKSNKALFILNPFAGIPPVKTIISKELKRRRNKLAWCKS
                     LSIDESGTLIRQNFDLYDIFIAAGGDGTVHTIATKLIGTDKSLGVLPIGSGNGFAKEL
                     GFKPSIRSLLQDIEQNENILVDVIEINDLLCLNVAGIGLDSFVAHSFDRLKRRGFWAY
                     VRLTLKNFFSLKPFRVNIEIEGEWTLSEDLFLLTIANTRQFGNRAYIAPGASPTDGKI
                     DLVLIRPFPRYLFPVFIFRLFTKSINRSKYVRTLKTDRKITISSEETRFHVDGEPVEI
                     TGKTVIRVRKGALKVLKTKYFTNEG"
     gene            complement(3325..5001)
                     /locus_tag="PK288_04525"
     CDS             complement(3325..5001)
                     /locus_tag="PK288_04525"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPM18216.1"
                     /translation="MTFLHEDAAILAAAFSTVEHKLPVWMAYVSVYLGIVTGDILIYG
                     LGHLAQQNKWLRSKIIGPKVERVHMWLDNHLVRVLVLCRITPGLLFPTFVACGWFRIP
                     FYRFASVSILAGAIYSSIVLTIVILFGDLVLNHLGYWAWGLAAFLLIAWALRNSFKSR
                     WSKTTEKAMGEIPPSFFAVFKKYMPGMRRKFHGMPSLDDLKKTVASAEHIPNSILYIP
                     VGLYYIFLGIRYRSLTLPSASNPKIETGGFMGESKASVMNMVGEDQKKWVSGFVTITR
                     NGIPDEADFENALKAMKEGNIGFPVVAKPDIGWNGYGVRLVENESHLQQYIANFPHNE
                     KIVLQRPVTYDGEAGVFYVRIPGEEKGKLYSITLRYFPFVTGDGISTLRQLIQKDQRT
                     KLRAGYYLGEKTEHVGFGDDDLEQIPREGELIRLSFLGSLRVGGLYRDASHMITPELT
                     ERFDQIARSIPEFHYGRFDIRFESVESLMKGEGFSIIEINGAGAEAIQAWDPNVSLFK
                     LYREFFKAYRLLFRIGDMNRARGFKPMPLRDFLRAIKHQNNLIDQYPPAG"
     gene            complement(5172..6797)
                     /gene="pruA"
                     /locus_tag="PK288_04530"
     CDS             complement(5172..6797)
                     /gene="pruA"
                     /locus_tag="PK288_04530"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPM18217.1"
                     /translation="MNNAIYSFREPRNEPILSYTRGSAERKLLEKELELQNNKVVEIP
                     LIIGGQEIRTGKTGKVTKPTDHGHILGTYHIAGEKEVRLAIESALAAKKDWMTMAWID
                     RASVMIKAAELISKKYRYVLNAATMLGQGKNVNQAEIDAVCETVDFLRFNAFFTSIIY
                     GEQPLSDKLSINRIEYRPLEGFVYTISPFNFTSIASNLNTSVALMGNTTVWKPATTSL
                     LSNYYLMKILKMAGLPDGVINFVPGSGPLISSVVMKHRDLAGIHFTGSNHTFNSLWKQ
                     VADNLSIYRSYPKIVGETGGKNFIVAHHSANPEELATAIARGAFEYQGQKCSAASRAY
                     IPASIWQTTKELLLNMIAEMKSGDVTDFRNFINAVIDENSYEKIMSYIKTAHDSRDCE
                     IIVGGKGDKSTGFFIDPTVILTTNPHFVTMQEEIFGPVITIYVYEDDRFEEILKLCDE
                     TSPFGLTGSIFSNDKYSVLQACNTLRYAAGNFYINDKPTGAMVGQQPFGGSRASGTND
                     KAGSFLNLIRWVSPRTIKETLIPSTDFRYPFME"
     gene            6997..8061
                     /locus_tag="PK288_04535"
     CDS             6997..8061
                     /locus_tag="PK288_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPM18218.1"
                     /translation="MTESKFYHISSSGLIYRVNTLEEAVKSLDEDGFIWLDYYNPEKK
                     DISILTDIIGIHPLSVEDCFDEKQVPKIEYFHNNTFILFNALSYKDKTLYLDEVNLFI
                     GSNFLITISGHDADGRMPLKNISIEAEHNIMNVKNGPAYLLHVVLDHLVDEKYIAFDT
                     MEDELEEAEETLLDDVSVFHPMQLVHLRRKLVSLRKSLYHEREILVKINRLDCPFIPE
                     KTMAQYRDIYDHLSKFFELTETYRELETSLMELYTSLLNNKMTLASNETNITVKRLTV
                     ITTVFLPLTLIASIGGMSEWTMITGGEANWKTSYAILLIFMIIMGGLSYYFIRAIEKG
                     IFLSGKKKRNENQSVSDMPA"
     gene            complement(8034..8258)
                     /locus_tag="PK288_04540"
     CDS             complement(8034..8258)
                     /locus_tag="PK288_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18219.1"
                     /translation="MSQTVEILILNNEMEAGLIDGLLNERNIPHMIRSFHDSVYDGLW
                     QMQSGWGQLLAPEEHRDEILKIYAGMSETD"
     gene            complement(8264..8803)
                     /locus_tag="PK288_04545"
     CDS             complement(8264..8803)
                     /locus_tag="PK288_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPM18220.1"
                     /translation="MQMNVSGEWIPVVDEEGKTISAAPRSVCHDGKSKLLHPVVHFHL
                     FNEKGDLFLQKRALTKDLLPGFWDTSAGGHMDPGENVDDALRREVLEELGISIFRYDL
                     NLRYIWESPVEKELVYSFKGSSNEPPVINRDEIDDGRFWPTEEIISNLGRGIFTPNFE
                     HEFTLIMKEFINYGNINSI"
     gene            complement(8953..11295)
                     /gene="pbpC"
                     /locus_tag="PK288_04550"
     CDS             complement(8953..11295)
                     /gene="pbpC"
                     /locus_tag="PK288_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPM18221.1"
                     /translation="MSRKFIIILTAAGMVIIPILLAPLPRFNNPLSTVVEARDHTLLG
                     ARIASDGQWRFPPGDAVPEKFRKAILTYEDRYFYFHPGVNPVSLIHAFVINSKHHEIR
                     AGGSTITMQTARLAGGNKPRTYLRKAIEIVSAIKLEMLRSKKKILEMYAANAPFGGNI
                     VGLDAAAWRYTGKLPSEITWAEAASLAVLPNSPALIHPGRNREKLREKRNSLLHRLKE
                     RNFIDSLTLELALEEPLLSEPDKLPSAAPHLTDYFNVTRKGERVRTSVDFRLQERVSA
                     IMELHQKKLEGNQIFNSAALVVSVESGEVLAYAGNSTTGNAAFHGGDVDIIRSQRSTG
                     SILKPILYAAMQQSGDLLPNTLIADTPARFPGFSPENFDKSYSGAVTAGTALSQSLNI
                     PAVRMLQEYSPEKFLELLRETGFTTFTMPAEHYGLSLILGGGETSLWELTGVYASMSR
                     ILRKAGQSQEYSRSDFHSPLILISDTKKSRGKDAGKAPLSAASVWLTYEALQKVNRPD
                     TEAGWQYFTSSRTLAWKTGTSFGFRDAWAVGSTPAYVIGIWVGNANGEGRPGLTGTAV
                     AAPILFDIAGLMETGPWFTPPWEEMTNIVVCTKSGYRAGPDCPDTREIPVCIEGLKSG
                     MCPFHSMVHLNKSRTYQVTADCAAPSEIVNEPWFILPPAMEFYYKRKHPEYRSLPPFA
                     KDCGGEKTGTFMEFIYPAPGLKIFIPRDHTGEPTKLVAEIVHRNPSRRVFWHIDESYL
                     GTTRNIHQVEILAGTGNHILTVYDEDGNRISSRFTIVGKN"
     gene            complement(11288..16861)
                     /locus_tag="PK288_04555"
     CDS             complement(11288..16861)
                     /locus_tag="PK288_04555"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPM18222.1"
                     /translation="MKKSRLIIPSVILIIISSCTGKENLPVKLLPKRVSDLQELNIPV
                     DKGFSEYITGYTSGIISVNSVFEVKFTPEFVAGINRKIPAGLFIFEPAVKGKAEWTDN
                     VTLAFKPARPLKPGTLYTVKLNLTKLAEVKENLGVFTSRIKTKKKDFVVSIGVLESSD
                     DGTKYTLHGNLVASDYVASTEAESYVQAKYGRKKLPIIWDHSDILEHRFSVINIERKD
                     KAGKVEVSWNGHFAGVKQKGSSAVKIPGTGVFAVTDLIFNRDEGQSIDIVFSDPLDAS
                     QETEGLIRFNPDIRMSARINANIVSLFPESRPGSPADIIVEESIKNFRGESLTSSFIR
                     RLDFTPVPPSVELTGNGVILPASYNLVLPFRTANLNAVDLRIIKIFGVNLPYFLQDND
                     INTSYSVKRFGRLIYSGRVDLINPPGTFSGSWNFHTIDLAEYIDVEPGILYRVELGFR
                     PSYSLYPCPDRTELEKYEESLEKSEEETNDYWDDPDNYYDDAGDMIYYSLGFNWNERN
                     DPCKPAYYSPDRKVTRNVLASNFGIIAKKGTDNSLLVMVNDLLTALPLSEVTIDVYDL
                     QMQLIVSRNTGHNGSVTMNCDRKPFLIIARKDRDRNYLKVTDGSSLSLSSFDVSGNKT
                     ENGIKAFIYGERDVWRPGDSIYLSIFLKDLNKNLPAGHPLQFELINPLEQRIDNQVQQ
                     TDGRNLLVFKTGTATDAVTGNYSAIFRVGGATFTRRVRIESIKPNRLKMELSFPGNLL
                     KQREGGTPGSLTVKWLNGNNAGNLRSSVEYRFKPAKTEFEKYRQYVFDDPSVDFYSET
                     VRMFEGTTDEKGEASVSFNPGTDIPAPGMLNAVFTVKAAEKGGDESITQKLYRYSPYE
                     TYVGLNLPGLKENERMIFTDTDHELRIATVDPQGKPVDTRVDIYFYRINYRWWWESDE
                     ENLASYISNNSYKPVLTHHLRTSNGTGSFRFNIGRNDWGRYLIRATAASGHSTGKIIL
                     IDWPWEYGMKSSADGATLLSVSTDKEKYAPGEEIRLSFPAPENSRAIITLENSSTVLE
                     EIRTPASKGNTVVSIKAKPGMAPNCYAFVTVIQPHAQTVNDMPIRLYGIVPVMVEDPA
                     TRLDPVISAPDEIRSQKSFDITVSEANRKPMTYTVAVVDEGLLDITAFRTPDPWSYFY
                     GREALGVRTWDVYDHVLGAFGGTLERLLATGGDEAITDRSAGKAQRFRPVIKFLGPFT
                     LQPGKSRTHTITLPQYTGSIRTMVIAGSERAFGSAEKSLLVRDPLMVLATAPRVLSPG
                     ERVTLPVTVFVQKSNITSLDLMAEAGSLIKFDEPVKSITVAGQGETDTRLVFTTAEKT
                     GKEVIKVTVSGGGETAVHEIEIDVRSPNPPEIRAVQKLLKRGEKWETSFNPFGIAGSD
                     AATLELSDLPAANFDKWLDYLLEYPHGCTEQIISAAFPQLLLHSIAGDGAKAEKSATN
                     IKEAVLKVVSRQMTGGGVAMWPGNYQPDNWVTSYAGHFMAEAERTGYSIPGNFRQKWT
                     RFQKKTAQDWRFDPKFRQSANDQAYRLFTLALAGDPERGAMNRMREIQNLPGLARWLL
                     AAAYATSGKPEVADRLLDMRNTGTEEEYSGYYYGSRMRDRAVILYTLTLLKKDAEGLP
                     LLRAICDELNTAGWFSTQSLSWGLFSYMKYSQLVSADKPGSAKISVSLNGEKSEISVP
                     EKKIFVKELQVRNGPNRLVVENISDQPVYANLVRKGIPLVSDATQTEKGLTMSVEYLN
                     MELKQIDHRNLVQGTDFMMVVKVTNKTFLNLDNIALTQMVPSGWEIQNTRLFEANYGI
                     QESVYDHRDFRDDRVNTYFSLNQGESKTYLLILNAAYKGEFHQPPVWCEAMYSESCYS
                     RIPGGKVKVTDNGIE"
     gene            complement(16953..18902)
                     /gene="dnaG"
                     /locus_tag="PK288_04560"
     CDS             complement(16953..18902)
                     /gene="dnaG"
                     /locus_tag="PK288_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPM18223.1"
                     /translation="MIDRQTIERILDAAQIVDVVQEFVPLKKRGVNYLGLCPFHNEKT
                     PSFTVSPSKEIFKCFGCGKVGNSVNFIMEHEHLTYPEALKYLAKKYHIEVTEKERTPE
                     EIEKQNERESLLVVTAYAARQFEENLFRSDEGKSVGLSYFMERGFRHSTLKKFETGYC
                     FDQRDAFSKQALEDGHRLDFLVKAGLTIQHEEKIFDRFSGRIMFPIHSLSGQILGFGG
                     RTLKADPKSAKYLNSPESEIYHKSRILYGIYQARKAITQEDKCFLVEGYTDVMSLHEA
                     AIENVVASSGTSLTREQVRLVKRFTQNITILYDGDPAGIKASIRGIDIVLEEGMNVKI
                     VLLPEGEDPDSYSKKVSNEQFLEYIKNNETDFIRFKTRLLLDEASNDPVKKAGLINDI
                     VRSVAVIPEAITRTIYIKECSNLLEVPESVLYQEVNKLMREKSFRERNKYPGPEDLPV
                     PPAGSPAKPVRPLTASFFAEKEVIRLLLKYGSLEFERKIRSEDGKEEIITVADYIVKE
                     ITSDELSFNDAVFARIFDDFRFSSEQGLFPDDKHFIKHEDPDISRISADLLADAHELS
                     RIWRDKQTYVETEEMKLHDIVPDVVLKFKSDKIQAYRKEIMILLEEAVRNNDRERIIF
                     LQTRFNNLSLALGHISKQLGNRILL"
     gene            complement(19068..19757)
                     /locus_tag="PK288_04565"
     CDS             complement(19068..19757)
                     /locus_tag="PK288_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPM18224.1"
                     /translation="MKNLVILTGAGMSAESGIRTFRETGGLWEEYDVTEVASPMGWQK
                     NRDLVLRFYNDRRTQLAACKPNAGHTGIAALEKYFNVNVITQNIDDLHERAGSTKVLH
                     LHGELTKARSTSDPSLVYDIGYKDIRPGDLCEKGSQLRPHIVWFGESVPMMDEAVRIT
                     SSADIFVVIGSSLNVYPAAGLMDYAPPKASLWLIDPNEVFVPARRHVGIIREPASTGV
                     EILTRHLMELR"
     gene            20058..20630
                     /locus_tag="PK288_04570"
     CDS             20058..20630
                     /locus_tag="PK288_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HPM18225.1"
                     /translation="MAENRKIIVAVTGASGSVYALSLIRRLAGLKSPPTEIATLFTET
                     AKQIWHHETGEAFHGMPPVRIYENNDFFVPFASGSSDYDTMIICPASMGTIGRIAGGI
                     SDDLVCRSADVMLKERRRLIIVPRETPYNLMHIRNMETLTLAGAVICPASPSFYSRPE
                     TINDLVMTVVERIISLAGFEADTYRWMKND"
     gene            20623..21072
                     /locus_tag="PK288_04575"
     CDS             20623..21072
                     /locus_tag="PK288_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPM18226.1"
                     /translation="MIDRRAVAFFRKHHVLTVATSVNGEPWCANCFYVYLEEKNALVF
                     TTDPATRHGREFRESPLVAGSVVLETRLIGRIRGIQFQAVVSEPQGDELELARKAYLK
                     RFPVAMLMETHLWIAELTLIKMTDNRLGFGKKLIWKKDGDSEVLNAG"
     gene            complement(21119..21718)
                     /locus_tag="PK288_04580"
     CDS             complement(21119..21718)
                     /locus_tag="PK288_04580"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPM18227.1"
                     /translation="MFSGIVEEAAPVVKLEKEKDNLHITLKCSFTGELKIDQSISHNG
                     VCLTVVKIHDDTYTVTAVRETLEKTNLGLLKPGDKVNLERSTKLDGRLDGHMVQGHID
                     QTAVCTSVTEAGGSWYYTFEYEPLKDDYITVEKGSVSVNGVSLTVVNSQPKSFQVAII
                     PFTYEITNFHLIEKGTVVNLEFDILGKYIAKIVRQVLGK"
     gene            22179..>22878
                     /locus_tag="PK288_04585"
     CDS             22179..>22878
                     /locus_tag="PK288_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPM18228.1"
                     /translation="MGKRFPEYKNLDLAQVNKDILKVWDANDTFRRSVREMNGRGEYV
                     FYEGPPSANGIPGIHHVLSRTIKDAVCRYKTQAGYEVKRKAGWDTHGLPVELGVEKAL
                     GITKEDIGTKISIDDYNKACRIDVMKYTDMWEELTRKMGYWINMEHPYVTYDNRYIET
                     LWWLLKQLYAGGYLYQGYTIQPYSPAAGTGLSSHELNLPGCYRDVRDTTCIAQFRLIR
                     DGHSEFLFRDTDTED"
BASE COUNT         5480 a         5453 c         5442 g         6503 t
ORIGIN      
        1 tccagtccct tgaccgccct ccatataatt tcattgaaaa gaaagtcgtt tatccggtcc
       61 tcggctgtga aatcaagctg ctcgctcgct ttttgccagg ggttttccgc aaggtttact
      121 tcatcgagat ccacaaggca tgattttacc ttaaatggag gatgatcagg gctgctgttc
      181 atgcatctcc agcgtggtgt ggcggcagca tcatattgag tcataggcgg gaggccgaga
      241 ataagctcca ttgttttcag tagggaagcg gttgtataag gtgtatgatc cacaaatcct
      301 tgtttcacat atcctcctgc aatatatgct gtgcttcggt gtgcatccac atgatcgggg
      361 ccattctggg catcatcttc cacaatgatt atcagggttt ccttccagat ggggctgtgg
      421 ctcagatagt caacgaacaa tcctgtggcc aggtcattgt cggcaacctg tgcaaaggga
      481 gttggcatat tcaggctaag ccccatggtg tgatcgttaa tgaatctgat tgtattgaac
      541 tgagggaccg catttattgc cagaagcgaa tcaaaatcct gtttccattc acgaaatctg
      601 acggtatcgc ggatggtctg gatccagggt gtgaaattcc tgcagaaatg atcctttaaa
      661 accggtatgg tcggttccct gtcgtttcca atgaattctc cgtaagtgcg gtagcttacg
      721 ccgtattttt tgcactggtc ccacagatat attttattat ttgcaatttc acgtgctcct
      781 tcgcccgggt agcctcctcc tcttccaccg tagcttgtcg gccagttttt ctcgaggtag
      841 tcggtggcat atgcacccat tgtccagctg tgtccgtcag cactgacctc accgttgaca
      901 tagaaattgt caaggaggac aaactcgcgg gccagggcat gctgattggg cgtaatgttt
      961 tctccaaaca gtaccagcga ggggtcgccg ttcccttcgg gcatgtcgcc gagtacctgg
     1021 tcatatgtac ggttttcctt gattacgtag aaaatgtatt taatgggaga ggggtcaccc
     1081 actttcaggg gtataggatt gccttcaggc gtatctgcgg ccagcagttc gttctccttg
     1141 ctgtaggggg tgttgttgta aacggccctc gtaaatattt tcatctgttt ttcatcaggg
     1201 atagagatca ggctcatggt acctttgaac aggcctccga tatactgaac ttcaacggcc
     1261 tgcacctttc ctcctgcctg gtaaattacc tctgtatcct tcctggcagg atttggcccg
     1321 tacggattgg ccatggaggt aacacctttt ccgttactga caaggatttt atcattcaca
     1381 accctgacgc atgtcggata ccatgctgtc gggataaatc ctttgcctct gctttcaccg
     1441 ggctgcgata catcgaaaac ggcaagacag ttgttatcgg catttgcaat gaacaaggtg
     1501 tttccgtctt catccagggc tacactgttt gttgtagatc cggcaggtga ttcgggatag
     1561 agtgcagtgt tgagggtttc aattaccttc atctgccggg tgtcaatgac tgacacactg
     1621 ttgtcgttgg catttgccac gtaaagataa tggccgttat gactgatgca catgtcgttg
     1681 ggattgtcac cgacaggaac cgaccccgtg atcctgtcgg caaacgtgtc ataaacaatt
     1741 atcttgtcac atccccagca tgaggcatat aaggtccggc ggtcgggtga aagtatacag
     1801 gtatatccct cgcctcccag gggatgaacc gaaatgacgg atctgaggcg cgtatcaatc
     1861 acataaaggg agttgttttc cttggtgacg gcataaaggc ggtttcttga atcgtcaggg
     1921 gcaattcctg caatagagat tttctccggc catggttcac ctgttttaat tgtgtcaggg
     1981 ttaacaagct tgttgcgctt cactgaatac ctgataatga agttgtcgtt gccacctgac
     2041 gcataaagat attttccgtc atcggaaaag gccagcccca gccatgattt ccctatttca
     2101 gtctcatagc aggaacttcc ctttttaatg tttatgagct gaattgtctg ggtgctctgt
     2161 ccgttgtttg tgacggcagc aagcttcctg tcaggagaaa ccgcaatgtt gagcggaagg
     2221 tcgccaagag gaataagttt tccgaccgga gtcagatgcc agccgttggg aagggagacg
     2281 cgctttgctt caatctcttc gggggtctgt ccatgaaggt tgaaaagcat tgcattaatg
     2341 actgccgctg tgagcaggca gaaaaacctg acgataaaat cagccttcat ttgtaaaata
     2401 tttggttttc aatactttga gagcaccttt tctgaccctg atgaccgtct tgcctgttat
     2461 ttccacaggt tctccgtcaa catggaatct tgtttcctcc gaggatatgg ttatcttcct
     2521 gtctgtttta agtgtcctta catatttcga cctgtttatg gatttggtga acaggcggaa
     2581 gatgaagacg ggaaaaagat atctggggaa tggtcgtatc aggacaaggt cgattttgcc
     2641 atcggtagga gaagctccgg gggcaatata tgccctgttc ccgaactggc gggtgttggc
     2701 tattgtgagt agaaaaaggt cttccgaaag tgtccattcc ccttcaattt caatgtttac
     2761 cctgaaaggt ttaaggctga agaagttttt cagggttaac cgcacgtacg cccagaatcc
     2821 ccgtctttta aggcggtcga atgagtgcgc gacaaagctg tcgaggccaa taccggccac
     2881 attgaggcag agcaggtcat ttatttcaat cacgtcgacc aggatatttt cattttgttc
     2941 gatatcctga aggagggagc ggattgaagg tttgaagccg agttcctttg caaaaccgtt
     3001 gcctgaacct atgggaagga ctccgagcga tttatccgtt ccgatcagtt ttgttgcaat
     3061 cgtatgtacc gttccgtcgc ctcctgcagc aatgaatata tcgtaaaggt cgaaattctg
     3121 ccttatcagg gttccggatt cgtcaatgct gagtgatttg caccaggcca gcttgttcct
     3181 acggcgttta agttccttag agattatggt cttcacagga ggaatgccgg caaaagggtt
     3241 gagaataaaa agggctttat tgctcttttg ttgatgatcc atcattaaac agctggcaaa
     3301 tcagagtaaa tttattaaaa aaagttaccc tgccggcgga tattggtcaa taagattgtt
     3361 ctgatgtttt attgccctta ggaaatccct gagcggcatg ggtttaaatc ccctggccct
     3421 gttcatgtcc ccgatcctga acagcagcct gtatgccttg aagaattcgc ggtaaagctt
     3481 aaatagtgaa acgttgggat cccaggcctg gattgcctcg gctccggcgc cgtttatttc
     3541 aattattgag aatccctcac ctttcatcag gctttccacc gattcgaacc tgatatcgaa
     3601 ccggccgtaa tggaattcgg gtatgcttct tgcaatctgg tcaaagcgtt cggtaagctc
     3661 gggtgtgatc atgtggcttg catcgcggta aagacctccc actctcaggc tgccaaggaa
     3721 tgacaggcgt atgagttcac cctccctggg aatctgttca agatcatcat caccgaaccc
     3781 aacgtgttca gttttttctc ccagatagta gccggctctc agctttgtcc gctgatcttt
     3841 ctggattagc tgcctgaggg ttgatatgcc gtctcccgta acaaagggaa aataccttaa
     3901 tgttattgaa tataactttc ccttttcttc tcccgggatc ctgacataaa aaacgcctgc
     3961 ttcgccgtcg taggtgacgg gtcgctgaag gacgatcttc tcattatggg ggaagtttgc
     4021 gatatactgt tgcaggtgtg attcattttc gacaagcctt accccgtagc cattccatcc
     4081 gatgtcgggc ttcgcaacta cggggaagcc tatattgccc tccttcattg cctttaaggc
     4141 attttcaaag tcagcttcat ccggtatccc gttgcgggtg atggttacaa agccggagac
     4201 ccactttttc tgatcttcac ctaccatatt cattaccgaa gcttttgatt cccccatgaa
     4261 accgccggtt tcaattttcg ggtttgatgc tgagggcagg gtcaggctcc tgtagcggat
     4321 gccaagaaag atatagtaaa ggcccacggg tatatacaga atactgtttg ggatatgttc
     4381 ggcggaagca accgttttct taagatcgtc gagggatggc atgccatgga actttcgccg
     4441 cattccgggc atgtattttt taaagacggc aaagaatgaa ggcggaatct ctcccatggc
     4501 tttttctgtt gttttgctcc agcgcgattt aaaagaattc ctcagtgccc atgctatgag
     4561 caggaaggcg gcaaggcccc aggcccagta tcccaggtga ttgagtacca ggtcgccaaa
     4621 aaggataacg attgtaagca caattgagga atagattgca cctgcaagta tggaaacgga
     4681 tgcgaagcgg taaaaaggga tcctgaacca cccgcatgcg acaaaggtgg ggaaaagcag
     4741 tccgggggtt atccggcaca gtaccagtac ccttaccagg tggttgtcga gccacatatg
     4801 tacccgttcc accttgggcc cgataatctt cgaacggagc catttgttct gctgggcgag
     4861 atgtccaagt ccatatatca gtatatcccc ggtgactatt ccgagataca cgcttacata
     4921 agccatccat acgggcagct tatgctcgac ggttgaaaaa gctgcagcaa gaatggcagc
     4981 atcctcatga agaaaagtca tgaaaaatat gacggcgcag tgttcgagcc aaaggagcag
     5041 atccggagat aacattaata aaacgtcata aattgaatgc ggttcttttt tctttcaaaa
     5101 caaaggtgcg taaaaatagc ttcatttatg atatcttgtt ctctccgggg tcaaaaaaaa
     5161 caaatcctgg attattccat gaacggatat ctgaaatccg ttgatggtat cagggtttcc
     5221 ttgatggtgc gggggctgac ccagcgtatg agattaagaa aacttccagc cttatcattt
     5281 gtacctgagg ctcttgatcc gccaaaaggc tgttgtccga ccattgctcc cgtgggtttg
     5341 tcgtttatat agaagttacc ggcagcgtat cttagtgtat tgcatgcctg caggacggag
     5401 tatttgtcgt tgctgaagat cgacccggtc agtccgaaag gagatgtttc atcgcataat
     5461 ttcagtatct cttcaaaccg gtcgtcttca taaacgtaga ttgttataac tggtccgaag
     5521 atctcttcct gcatggtcac aaagtgagga ttggttgtta ggatgacagt aggatcgatg
     5581 aagaaaccgg ttgatttatc tcccttcccg ccgacaatga tttcacaatc acgtgaatca
     5641 tgtgcagttt ttatgtaact catgatcttt tcataggagt tttcgtcgat aacggcatta
     5701 atgaagttcc tgaaatcagt gacatcgccc gatttcattt cggcaatcat attcaggagc
     5761 agttccttcg tcgtttgcca gatggaggcc ggtatgtaag cccgcgatgc ggcagagcat
     5821 ttctgtccct gatattcaaa ggcgcccctg gctatggctg ttgcaagttc ttccggattt
     5881 gcagaatggt gtgcgacaat gaagttttta cctcccgttt caccgacaat cttagggtac
     5941 gatctgtaga tgctgagatt gtcggcaacc tgtttccaca agctgttgaa ggtatggttc
     6001 gaaccggtga aatgaattcc tgcaagatcc ctgtgtttca taaccacgct gctgataagc
     6061 gggcctgatc cgggtacgaa attgatcact ccgtcgggca gccctgccat tttcaggatt
     6121 ttcatcagat agtaatttga aagcagggag gtggttgccg gtttccagac cgtggtattt
     6181 cccattaggg caacactggt attaaggttg gaagctattg aagtaaagtt gaaaggagaa
     6241 attgtgtaaa caaatccctc cagtggccgg tattcgatcc ggttaatact gagcttgtcg
     6301 gacaatggtt gttcgccata gattatggag gtgaaaaagg cattgaacct gagaaaatct
     6361 acggtttcac aaaccgcatc tatctcagcc tgattgacat ttttcccctg gccaagcatt
     6421 gtggcggcat tcagaacata cctgtacttc tttgaaatca gctcggcagc tttgatcatg
     6481 acagaggccc tgtcaatcca agccatggtc atccagtctt tttttgcagc cagtgctgac
     6541 tcaattgcca gtctgacctc cttctcaccg gcaatatgat atgttccaag aatatgacca
     6601 tggtcagtcg gctttgtcac tttcccggtt ttcccggtac ggatctcctg cccgccgatg
     6661 atcagcggga tctcaacaac cttgttgttc tgcagctcaa gctctttttc caggagcttt
     6721 ctctccgcac ttccccgagt ataggaaagt atgggttcgt tccttggttc cctgaaactg
     6781 taaatggcgt tgttcatggc attgatattt ggttctcaga agattaacat tgcacactta
     6841 ttttggttca ggctagcctg aatgtctggc tgttatacaa ctacactcaa tctaaattgc
     6901 ttatccccct tcgtccggca tcaaactttt tcgtaatttg gatacttctt taatcaaata
     6961 tcagctttgt ttttttaata attaaagaca ttgatcatga ctgagtcaaa attctatcac
     7021 atttcttcct cagggctcat ttacagggtc aacacgctgg aagaggcagt taaatcactg
     7081 gatgaggacg gttttatatg gctcgactat tacaaccccg aaaagaagga tatcagcatc
     7141 ctgacagaca ttattggtat tcacccttta tctgtggaag actgctttga tgaaaagcag
     7201 gttcccaaga tcgagtattt tcacaacaac accttcattc ttttcaatgc tctcagctat
     7261 aaggataaaa ctctctacct ggatgaagtt aacctgttca taggcagcaa ctttctcatc
     7321 acgatcagcg gtcatgacgc tgacggaagg atgccgctga aaaacatttc aatcgaggct
     7381 gagcataaca tcatgaatgt aaaaaatggc cccgcatatc ttttgcatgt tgtgcttgat
     7441 caccttgtgg atgagaaata tattgccttc gataccatgg aagatgaact cgaggaagct
     7501 gaagaaacac tcctggatga tgtttctgtt ttccacccca tgcagcttgt tcatctgaga
     7561 aggaagctgg taagcctgcg taaaagtctt tatcatgagc gtgagatcct ggtaaagata
     7621 aacaggctcg actgtccctt cattcctgaa aaaacaatgg ctcagtaccg ggacatttat
     7681 gaccaccttt caaaattctt tgagctgact gagacataca gggaactgga aacgagttta
     7741 atggagcttt acacttcgct tttgaacaat aaaatgacgc ttgcttcaaa tgaaacaaat
     7801 atcactgtta aacgattaac agtgataacc acggtattcc tgccccttac cctcattgcc
     7861 agcattggcg gaatgtcaga atggacaatg ataaccggag gggaggccaa ctggaagacc
     7921 tcctatgcca tattgttaat ttttatgata attatgggcg ggctgagtta ctatttcatc
     7981 cgggcgattg aaaaaggaat ctttctgtcg ggaaagaaaa aacgaaatga aaatcagtca
     8041 gtttctgaca tgccggcata gattttcagg atctcgtccc tgtgttcctc tggagccagg
     8101 agctggcccc agcccgactg catctgccac aggccgtcat acacagaatc atggaacgac
     8161 ctgatcatgt gagggatgtt cctttcattc agcagcccgt caataagacc ggcttccatt
     8221 tcattgttaa gtatgaggat ctcaacggtt tgactcataa taatcagatt gaattgatat
     8281 ttccgtaatt tatgaattct ttcattatca aagtgaattc atgttcaaag ttaggtgtga
     8341 aaattccacg gccgagattt gagatgattt cttcagttgg ccagaacctt ccatcgtcaa
     8401 tttcatccct gtttataacc ggcggttcat tcgaggagcc tttgaagctg tatacaagct
     8461 ccttttcaac aggagattcc catatatacc tcaggttcag atcataccta aaaatactga
     8521 ttccaagctc ctcaagcacc tcccttctga gagcatcatc gacattctca cccggatcca
     8581 tatgaccacc ggctgatgtg tcccagaaac cgggcagaag atcttttgtc agcgcgcgct
     8641 tctgaaggaa cagatctcct ttctcattga acagatgaaa atgtacaaca ggatgcagca
     8701 gcttgctttt tccgtcgtgg caaacactgc ggggcgcggc cgatattgtt tttccttcct
     8761 cgtcgacaac cgggatccat tccccggaaa cattcatttg catgagcctg cttttattga
     8821 ggaacaaata tggtcacgga attgttatga aacaagtcaa aagattgagc ttattactcc
     8881 gggggcagat catcaggtgt aaccggctga attacagtaa acgctacggg tacaccaaca
     8941 aaattcaaaa gttcagtttt taccgactat tgtaaaccgg gaactgattc tgtttccatc
     9001 ttcatcatac acagtcagaa tatggtttcc tgtaccggca aggatttcga cctggtgaat
     9061 attcctggtt gtacccaggt agctttcatc aatatgccag aatacccgtc tggaaggatt
     9121 tctgtggaca atttcggcaa caagcttggt aggttcgccg gtatgatcgc gcggtatgaa
     9181 gatttttaat ccgggtgcgg gatagatgaa ttccatgaat gtgccggttt tttcgccgcc
     9241 acaatctttc gcaaaaggag gcagggacct gtattccggg tgttttcttt tgtagtagaa
     9301 ttccattgca ggaggaagaa taaaccatgg ttcattgact atttctgaag gtgcagcgca
     9361 atctgcggta acctggtacg tgcgtgactt gttgaggtga accattgaat gaaacggaca
     9421 catcccgctt ttaaggcctt cgatgcatac cgggatctcc cttgtatcgg ggcaatcagg
     9481 tccggccctg tagccgcttt tggtgcagac cacaatattt gtcatttcct cccatggagg
     9541 ggtaaaccag ggaccggttt ccatcagacc tgcaatgtca aataggattg gtgctgcgac
     9601 ggctgttccg gtgagtcccg gcctgccttc cccattggca ttgccaaccc atattccgat
     9661 aacataggca ggggtacttc ccacggccca ggcatccctg aagccaaaac ttgtgccagt
     9721 tttccatgca agggtcctgg atgatgtgaa atactgccat cctgcttccg tatcgggacg
     9781 attaactttc tgcagcgctt cataagtcag ccatacagaa gcggcagaca ggggagcttt
     9841 gccggcatct ttccctctgg atttcttcgt gtcgctgatc aatatcaacg gagagtggaa
     9901 gtccgatcgg gaatattcct ggctttgccc cgccttcctg agtatccttg acattgaagc
     9961 atagactcca gtgagttccc acaatgaagt ttcaccacct cccaggataa gcgagagacc
    10021 gtaatgctcc gcaggcatgg tgaaagttgt gaatcctgtt tccctgagca attctagaaa
    10081 tttttcaggg ctatattcct gcagcattct gactgccggg atgttaagtg attgcgagag
    10141 ggcggttcct gcggttacgg caccgctgta actcttgtcg aaattttcgg gtgagaaacc
    10201 cgggaagcgt gcaggagtat cggcaattag tgtgttgggc agcagatccc ctgattgctg
    10261 cattgctgca tagagtattg gtttaaggat acttcctgta cttctctgtg atctgatgat
    10321 atcgacatca ccaccgtgaa atgcagcatt tccggttgtg ctgttgccgg cataggccag
    10381 aacctccccc gactccacac ttaccaccag ggctgcggaa ttgaagatct ggttgccttc
    10441 aagttttttc tgatgcaatt ccattattgc agagaccctc tcctggagcc tgaaatcgac
    10501 agaggttctc acacgttctc cttttcgcgt cacattgaaa taatcagtga ggtgaggagc
    10561 tgctgacggc agtttgtcgg gttcggaaag aagcggttct tcaagtgcca gttcaagggt
    10621 gagggagtcg ataaaattcc tttctttcag gcggtgtagg aggctattgc gtttttccct
    10681 gagtttttcc cggttccttc ccggatgaat aagagcgggt gaattgggaa gcactgcaag
    10741 cgaagcggct tccgcccagg tgatctcgga aggcagtttg ccggtgtacc tccaggcagc
    10801 tgcatcaagt ccgacgatat tgcctccgaa aggagcattt gcagcataca tttcaaggat
    10861 cttcttcttt gatctcagca tttcaagctt gatggcagaa acgatctcga tagccttcct
    10921 gagataggtg cgtggtttgt taccccccgc cagcctcgct gtctgcattg tgatggtcga
    10981 acctccggcc ctgatctcat gatgcttcga gttgatgacg aaggcgtgta tgagcgatac
    11041 gggattaacc cccgggtgaa aatagaaata cctgtcctca taggtaagta ttgctttcct
    11101 gaacttttcc gggacggcat cccctggagg gaatctccat tgtccgtcgg aggcgatcct
    11161 ggcaccgagg agtgtgtgat cccgggcctc cacaaccgtc gacagcggat tattgaatcg
    11221 gggcaaaggg gcaagcaaaa tcgggatgat caccatcccg gcagcagtca gtatgattat
    11281 gaacttccta ctcaatgccg ttatccgtga cttttacctt gccgcccggg atacgtgaat
    11341 agcagctttc cgaatacatg gcctcgcacc aaaccggagg ctgatggaat tcccctttgt
    11401 aagcggcatt gaggataagc aaataagttt tgctttcacc ctggttcagg ctgaaatagg
    11461 tgttcacccg gtcgtccctg aaatcgcggt ggtcataaac gctttcctgt attccgtaat
    11521 tggcctcaaa cagccgggtg ttctgtattt cccatcccga ggggaccatc tgtgttaagg
    11581 ctatattgtc gagattcagg aacgttttat tcgtcacttt cacaaccatc atgaaatcag
    11641 ttccctgtac caggtttctg tgatcaatct gcttcagttc catgttgagg tactcaacac
    11701 tcatggtcag tcctttttcg gtctgggtgg catcagaaac cagcggaatc cctttcctga
    11761 ccaggtttgc ataaacgggt tggtcagaga tgttttccac aacaagcctg ttggggccat
    11821 tcctgacctg gagctctttg acgaagattt tcttttcggg aacggaaatc tcggattttt
    11881 caccgttaag ggaaacgctg attttcgccg aacccggttt gtcagcggag accagctgag
    11941 agtatttcat gtatgaaaac aatccccacg acaatgactg ggtgctgaac cagccggcgg
    12001 tattcaactc gtcacagatt gctcttagca ggggaaggcc ttcggcgtcc tttttcagta
    12061 gtgtgagggt atataggatc acggccctgt cgcgcatgcg gctgccgtaa tagtatccgg
    12121 aatattcttc ttcggttccg gtattgcgca tgtcgagcag cctgtcagcg acttcaggtt
    12181 tgccggaggt agcataggcc gctgcgagaa gccatctggc caggccgggg agattctgaa
    12241 tttccctcat cctgttcatt gcacctcttt ccggatcgcc ggcaagggca agagtgaaga
    12301 gcctgtatgc ctgatcgttg gctgattgcc tgaatttcgg gtcaaagcgc cagtcctgtg
    12361 cagtcttttt ctgaaacctt gtccatttct gcctgaaatt tccgggtatg ctgtatcccg
    12421 ttctttcagc ttccgccata aagtgacccg catatgatgt tacccagtta tcgggctggt
    12481 agttgccggg ccacattgcg actcctccgc cggtcatctg cctggagacc acttttaaaa
    12541 cagcttcctt aatatttgtt gcagactttt cggcctttgc cccgtctcct gcaatgctgt
    12601 gaagcaataa ctgggggaaa gccgcagaaa taatctgttc ggtgcagccg tgcggatatt
    12661 caagcaggta atcaagccat ttatcaaaat tagcagcagg aagatcggat aattcaaggg
    12721 tggcagcatc ggatcctgcg atcccgaaag ggttgaatga ggtttcccat ttttcacccc
    12781 gtttcagcaa tttctgcacg gctctgatct ccggtggatt cgggcttcgg acgtcgattt
    12841 caatctcatg gacggctgtt tccccgcccc ccgatacagt aacctttatc acctcctttc
    12901 ctgttttttc ggcagtggta aacacaagcc gggtatccgt ttccccctgg ccggcgacgg
    12961 tgatgctttt caccggctca tcgaacttta tcaggctacc ggcttctgcc atcaggtcaa
    13021 gactggttat attgcttttc tgaacaaaaa cagtaaccgg caatgtgacg cgttctcccg
    13081 ggctcaggac cctgggggcg gtcgcaagta ccatcagcgg atccctcacc agcaggctct
    13141 tttcggcact gccgaatgcc ctttcactgc ctgcgatcac cattgttctt attgaccccg
    13201 tatactgggg aagggtaatg gtgtgggtcc tggatttacc cggctgaaga gtgaagggtc
    13261 cgaggaattt tatcaccggc ctgaacctct gcgccttgcc cgccgatctg tcagtaatgg
    13321 cttcatcgcc tcccgtggca agaagccttt ccagggttcc cccgaaagca cctagaacat
    13381 ggtcgtatac atcccaggtc ctgacgccca gggcttcacg gccgtaaaaa taactccacg
    13441 gatcgggtgt cctgaaagca gtaatatcca ggagtccttc gtcaacaaca gccacggtgt
    13501 atgtcatcgg cttcctgttt gcctcactca cggtaatgtc aaaacttttt tgagagcgga
    13561 tctcatcggg tgcggagatt accggatcca ggcgggttgc aggatcctca accataacag
    13621 ggacaatacc gtaaagacgt atgggcatgt cgttcacggt ttgtgcatga ggctgtatta
    13681 ctgttacgaa tgcatagcaa ttaggagcca tgccgggttt tgccttaatg cttacgacag
    13741 tgttcccctt tgaagccggg gtccggatct cttccagtac tgttgacgaa ttttcaaggg
    13801 tgattattgc cctggaattt tcgggtgcgg gaaagcttag ccgtatttct tctccaggcg
    13861 catatttttc cttatcggta ctcaccgaca gcagtgttgc cccgtccgca ctgcttttca
    13921 tcccgtattc ccacggccag tcgataagta tgattttacc cgttgaatgt ccgctggcgg
    13981 ctgtggcgcg gataaggtat ctgccccagt cattccttcc tatattgaac cggaatgagc
    14041 ctgtaccgtt actggttcgc agatgatggg tcagcacggg tttgtagctg ttgtttgaaa
    14101 tgtacgatgc aaggttttcc tcgtcagatt cccaccacca cctgtaattt atcctgtaga
    14161 agtaaatgtc cacccgggtg tcaaccggtt ttccctgggg gtcgactgtg gcaattctta
    14221 actcatggtc ggtgtcggta aaaatcattc tttcattttc cttgaggccc ggaagattca
    14281 gacccacata agtctcatac ggagaatacc ggtagagttt ctgtgttatg ctttcatctc
    14341 cacccttttc tgcggctttt accgtgaaaa ccgcattaag cattcccggg gcaggtatat
    14401 cagtgccggg attgaaactg acgcttgcct cacctttctc atcggtggtt ccttcaaaca
    14461 tcctgactgt ttcagaataa aaatcgactg aaggatcatc aaaaacatac tgtctgtact
    14521 tttcaaattc cgtttttgca ggtttgaagc ggtattcgac tgatgatcgc aggttgcctg
    14581 cattgtttcc attgagccat tttacagtta gtgagccggg agtccctcct tctctttgtt
    14641 taaggaggtt accgggaaat gaaagttcca ttttcagcct gttgggctta attgattcaa
    14701 tcctgaccct cctggtgaat gtggcaccac cgaccctgaa tatggcggaa taattgccag
    14761 tgacggcgtc ggtggcggtt cctgttttaa agacaagaag gttcctgcca tcggtctgct
    14821 gtacctggtt atcaatcctt tgttcaagcg gattgatcag ttcaaactgc agcggatgtc
    14881 cggccggaag atttttgttc aggtccttca ggaatatgga aagataaatg gaatcaccgg
    14941 ggcgccagac atctctttca ccgtaaatga aggcctttat tccgttctcg gttttatttc
    15001 ccgaaacatc gaaggaactc agcgacaggg atgaaccatc ggttacctta aggtagttcc
    15061 ggtcccggtc ctttcttgct attataagga aaggctttct gtcgcaattc atggtaacgg
    15121 aaccattgtg gccggtgttc cgggaaacaa tcagctgcat ctggagatca tacacatcga
    15181 ttgtcacttc gctgagcggg agtgctgtaa gaaggtcatt taccattacg aggagactgt
    15241 tgtcagttcc ttttttggca ataatgccga agttagatgc cagtacgttc ctggtgactt
    15301 ttctgtcggg gctgtaatag gctggtttgc aaggatcatt gcgttcattc cagttgaatc
    15361 ccagtgaata gtagatcata tccccggcgt cgtcataata attgtccgga tcatcccagt
    15421 aatcatttgt ttcttcctcc gacttctcaa gagattcttc gtatttttcc agttcagtcc
    15481 tgtccgggca gggataaagc gagtaagagg gccggaagcc aagttcgacc ctgtacagta
    15541 tacccggttc gacatcaata tattcggcaa gatcaatcgt atggaagttc cacgatccgg
    15601 aaaaagtacc cggtggattg atcagatcaa cccggcctga ataaatgagc cttccgaacc
    15661 tctttaccga ataggatgtg tttatatcgt tgtcctgcag gaaatacgga agattgaccc
    15721 cgaatatctt gatgatcctg agatcgacgg cattcaggtt ggctgtcctg aaaggcagta
    15781 ccagattgta tgaagcaggg aggatcactc cgtttcctgt gagctccacc gatggtggga
    15841 ctggtgtaaa atccagtctt ctgatgaatg acgaagtcaa tgattcaccc cggaagtttt
    15901 taattgattc ctcaacgata atgtctgccg gactcccggg tcttgattcc ggaaaaaggc
    15961 tgacaatatt tgcattgatc ctggctgaca ttcttatatc cgggttaaag cggatgagac
    16021 cctccgtttc ctgagaagca tcgagcgggt cagagaagac aatatcaatg ctctgcccct
    16081 cgtcacggtt gaagatcagg tccgtaactg caaatactcc cgttccgggt atcttcactg
    16141 ccgagctgcc tttttgtttt acccctgcaa aatgtccgtt ccagcttacc tctaccttcc
    16201 cggccttatc ctttctttcg atgttgatta cagaaaatct gtgctcaaga atatcggaat
    16261 gatcccagat tattggcagt tttttacggc catattttgc ctggacataa gactcggcct
    16321 cagtggatgc tacatagtct gatgccacca ggttaccgtg cagtgtgtat tttgtaccgt
    16381 catccgaact ttccagcacc ccgatggaaa cgacgaagtc cttctttttt gttttaatcc
    16441 tggaagtgaa tacccccaaa ttttccttaa cttctgcaag ttttgtaagg ttaagcttaa
    16501 ccgtatacaa tgtccccggc ttcagcggcc tggcaggttt aaaagcaaga gttacattgt
    16561 ctgtccattc ggctttccct tttacggcag gttcaaaaat gaacaatccg gcaggaatct
    16621 ttctgtttat cccagctaca aattccgggg tgaattttac ctcaaaaaca gaattgaccg
    16681 atatgatgcc ggaagtgtat ccggtaatat actcactgaa tcctttatcg acgggaatat
    16741 tcagttcttg caaatcggaa actcttttcg ggagaagttt aaccggcaga ttttcttttc
    16801 ccgtgcatga tgagatgatg atcaggatga cagaagggat gattagtctt gattttttca
    16861 ttttcgggaa tgattatttc aggatgacgg atttgcctga gttcaacggt gacatattgt
    16921 tacattgcta aaaatacgaa aaagtcggaa tcctaaagta aaatcctgtt gccaagctgt
    16981 tttgaaatat gccccagtgc caggctgaga ttattgaatc ttgtctgcag gaaaatgatc
    17041 ctttcccggt cattgtttct taccgcttct tcaagaagga tcatgatctc ctttctgtag
    17101 gcctggattt tgtcgctttt gaatttcagc accacatcag ggacaatatc atgaagtttc
    17161 atttcttcag tttcaacata ggtttgcttg tctctccata tcctgcttaa ttcatgtgca
    17221 tctgcaagga ggtcggcgga gatccttgag atgtccggat cttcatgttt gataaaatgt
    17281 ttgtcatcgg ggaaaaggcc ctgttcggaa ctgaacctga aatcgtcgaa gatccttgca
    17341 aatactgcat cgttaaatga cagttcatct gatgttattt ctttcacaat atagtcggca
    17401 acggttatta tctcctcttt accatcctca ctgcggattt tcctttcaaa ttccaggctt
    17461 ccgtatttca gcagcagtct tatcacttcc ttttccgcaa aaaagcttgc cgtaagcggt
    17521 cttacaggct tcgcaggact tcctgccggc ggaacaggaa ggtcttccgg ccccggatac
    17581 ttattccttt cacggaaaga tttctcacgc atgagtttgt tgacctcctg ataaaggacg
    17641 gattccggaa cctcaagcag attgctgcat tccttgatgt atattgttct tgttatggct
    17701 tcgggaatta ctgcaaccga tctgacaatg tcatttatca ggccggcttt tttgacagga
    17761 tcgttgctgg cctcatcaag cagcagtctg gttttgaacc ttataaaatc agtctcgtta
    17821 ttttttatat attccaggaa ttgctcattg cttacttttt ttgagtatga atcgggatct
    17881 tcgccttcgg gtaaaagaac tatcttaaca ttcattcctt cttcaaggac aatatcgatt
    17941 cccctgattg aagccttgat accggccgga tcgccatcat agaggattgt aatattctgg
    18001 gtgaaccgtt ttaccagcct tacctgttcc cgggtaaggg aagttcctga gcttgctacc
    18061 acattttcaa tggcagcctc atgaagtgac atcacatccg tataaccttc gacaagaaag
    18121 catttgtctt cctgtgtgat tgccttgcgt gcctggtaga tcccgtacag gatcctgctt
    18181 ttatgataga tctctgactc cggtgaattg agatatttgg cacttttagg gtcggctttc
    18241 agagttcttc ccccgaaccc aaggatctgt cccgacaacg aatggatcgg gaacattatt
    18301 cttccgctga agcggtcgaa tattttttct tcgtgttgaa ttgtcagtcc tgccttcacc
    18361 aggaaatcga gcctgtggcc gtcctcaaga gcctgttttg aaaaagcgtc cctctggtcg
    18421 aaacagtagc ctgtctcaaa ttttttcagg gtactgtgac ggaatcccct ttccatgaaa
    18481 tatgaaagtc cgactgattt tccctcatcg gaacggaaca ggttttcttc aaactgcctt
    18541 gcagcatatg cagtgacaac gagcagactc tccctttcat tctgtttttc aatttcctca
    18601 ggggtgcgtt ctttctccgt tacctcgata tggtattttt ttgcaagata tttcagcgcc
    18661 tcaggataag tgagatgctc atgctccatt ataaaattga cggagttccc gacctttccg
    18721 cagccaaagc atttgaatat ctcctttgac ggagataccg tgaatgaagg ggtcttttca
    18781 ttgtggaagg ggcacaatcc aaggtaattc actccccgtt ttttcagggg gacaaactcc
    18841 tgcacaacgt cgacaatctg tgcagcatca agtatccgtt cgattgtctg cctgtcaatc
    18901 atggatcaaa gatatcagga aaaccgtgaa aggtcaaaag taccttcgga gaatatatta
    18961 acaaaccagc ttgtacggta tccttcagtc atgcagatta ttggcatcag ggaagtatga
    19021 ttatgggcag gacgcaggcc ttactgacgg gtataaaggc aattaaatca tcttagttcc
    19081 atcaggtgcc gggtaagtat ttcaactccc gttgaggcag gctcccttat gatccctaca
    19141 tgccgcctgg caggaacaaa cacctcattc gggtcgataa gccagaggga tgccttcggg
    19201 ggtgcataat ccattagtcc cgcagcaggg tagacgttga gtgatgaacc gataactacg
    19261 aaaatatcgg cggaagaagt tatccttaca gcttcgtcca tcattggcac agattctccg
    19321 aaccagacaa tgtggggcct gagctggctc cctttttcac acagatcgcc cggccggata
    19381 tccttgtacc cgatgtcgta taccaacgac gggtcgctgg tgcttcttgc cttggtaagt
    19441 tccccgtgta gatgcagcac ttttgtactt cccgccctct catgcaggtc atcgatattc
    19501 tgtgttataa cattaacatt gaaatacttt tcaagagcgg caattcccgt atgacctgcg
    19561 ttgggtttac aggccgcaag ctgggtccgg cggtcattgt agaacctgag caccaggtcc
    19621 ctgttttttt gccaacccat cggagaggcg acttcggtga catcatactc ttcccagagg
    19681 ccgcctgttt cgcggaatgt cctgataccg ctctcggcac tcattcctgc acccgtcaga
    19741 ataaccagat ttttcattcg gcaataatta attcattgtg cattactgat cccggctgtt
    19801 gatgtatgaa tgccggctca tcaccggccg taccggaaga cctgctgatg tgaaccaagg
    19861 atggatctcc gggatttcgg ccggaaaaca tccggcatat gaagatagta aataacggtt
    19921 aaatataaaa acaatcccgg ctgcccctga tgagtgcatt ttggattccc ggtccgcgtc
    19981 aaatgcacgg catggtacgg gatcatttcc aaacactata tttattactt ttacggtttg
    20041 atcattaata cagggtgatg gctgaaaaca gaaaaatcat tgttgcagtt acgggtgcaa
    20101 gcggttcagt ctatgcacta tcacttatca ggaggcttgc cgggctgaaa tcgccgccta
    20161 cagagatagc caccctgttc acggagactg caaaacagat atggcaccat gaaaccggag
    20221 aggcatttca cggcatgcct ccggtgagga tttatgagaa caatgatttt tttgttccgt
    20281 ttgcctccgg atcgtctgat tatgatacca tgataatttg tccggcctcg atggggacca
    20341 tcgggaggat tgcgggcggg atctcggatg atcttgtctg ccggtcggcg gatgtgatgc
    20401 tgaaggaaag gcgcaggctg atcattgttc caagagagac cccttataat ctgatgcata
    20461 tacgtaatat ggagaccctt accctggcag gggcagtgat atgtcctgcc agtccgtcat
    20521 tctacagccg gcccgaaaca ataaacgacc tggtaatgac tgttgtggaa aggatcattt
    20581 cacttgcagg atttgaagca gatacttacc gctggatgaa aaatgattga tcgccgggca
    20641 gttgcttttt tcagaaaaca ccatgttctg accgttgcaa caagtgttaa cggcgaaccc
    20701 tggtgcgcca attgctttta tgtttacctc gaagaaaaaa atgcacttgt atttaccacc
    20761 gatccggcaa cgaggcatgg gagggaattc agggagagtc cgctggtggc gggttccgtg
    20821 gttcttgaga ccaggctcat cggcaggatc agggggatcc agttccaggc agtcgtgtct
    20881 gagcctcagg gcgatgagct ggagcttgcc cgaaaagcgt atctgaaacg gtttcccgtt
    20941 gccatgctca tggaaaccca tctgtggatt gcggaactca ctcttataaa aatgaccgat
    21001 aaccggcttg gctttggcaa aaagctgatc tggaaaaagg atggtgacag cgaggttctg
    21061 aatgccggat agggccaatg ttttccaggc gatggagctc cgcaggtgta tcggccggtt
    21121 atttcccgag tacctgcctg actattttgg caatgtattt gcccaatatg tcgaattcga
    21181 ggttcaccac tgtccctttt tcgatcaggt ggaaatttgt aatctcgtag gtgaaaggaa
    21241 tgatggcaac ctggaaagat ttcggctgcg agttcacgac tgtcaggctc accccgttaa
    21301 ctgaaactga tcccttttca acagtaatat aatcatcctt cagcggttca tattcaaatg
    21361 tatagtacca gcttcctccg gcctccgtca ctgaagtaca gaccgcggtc tggtcgatgt
    21421 ggccctgaac catgtggccg tcgagcctgc cgtcgagctt cgtgcttctc tcaagattaa
    21481 ccttgtcgcc cggtttaagc agtccgagat ttgtcttttc cagggtttcc cttaccgctg
    21541 tcacagtata tgtgtcatcg tggattttta ccacggtaag acagaccccg ttgtgggaaa
    21601 tgctctgatc gatcttaagt tcgcctgtaa acgagcattt cagggtgata tggagattgt
    21661 ctttttcctt ttcaagcttt accacaggtg ctgcttcttc tacaattcct gaaaacatga
    21721 ctttattggt ttttatccgc tgcaaaggta tttaaaaacg tttagaagaa gtttctctga
    21781 accgggtttt acagtcaacc ctttcctgtc ggtctcttcc ggagatattt acaggcagca
    21841 ccgtgatccg ttgatcgtac ccgtcattcg catccccgtc attccgcatc tacgctgact
    21901 tgctctctca tcccctcatc ctctcatccc ctcatcccct catcctctca tcccctcatc
    21961 ccctcatcct gttatcctct catcctctta tccccccatc ccctcatcct ctcatcccct
    22021 catcctctta tcctgttatc ctctcatcct cttatccccc catcccctca tctccacatc
    22081 tcctaacaaa ttaaacttcc tgcattgtcc ccttgatcct tttttcatat ctttgtgcga
    22141 ttttttaaaa tattctcaaa aaacaacttg ttgatccgat ggggaaacgt tttccagaat
    22201 acaaaaacct tgatctggcc caggtaaata aggatattct gaaggtatgg gatgcaaatg
    22261 acactttccg cagaagtgtc agggagatga acggcagggg tgagtatgtt ttttacgaag
    22321 gaccgccgtc tgccaacggc attccgggta ttcatcatgt tttgtcgcgc actatcaagg
    22381 atgcagtttg caggtataag actcaggcgg gttatgaggt gaagcgtaag gctgggtggg
    22441 atactcatgg tctgcctgtt gagctgggtg ttgaaaaggc gcttggcata accaaggagg
    22501 atatcgggac gaagatctcg attgatgatt acaacaaagc ctgccgcatt gatgtgatga
    22561 aatatactga catgtgggag gagctcaccc gcaagatggg atactggatc aacatggagc
    22621 atccctatgt aacatatgac aaccgttata ttgaaacctt gtggtggttg cttaagcagc
    22681 tttatgccgg cggctatctg taccagggtt acacgataca gccgtattca ccggctgccg
    22741 ggaccggact aagttcgcac gaactgaacc tgcccggctg ttacagggat gtcagggata
    22801 cgacatgcat tgcacagttc aggcttatcc gcgacgggca ttcggaattc cttttcaggg
    22861 acacggatac agaagatg
//
LOCUS       DAOOYG010000032        22598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2118_length_22598_cov_6.512753, whole genome shotgun sequence.
ACCESSION   DAOOYG010000032 DAOOYG010000000
VERSION     DAOOYG010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2118_length_22598_cov_6.512753"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1799
                     /locus_tag="PK288_04590"
     CDS             78..1799
                     /locus_tag="PK288_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPM18229.1"
                     /translation="MKNYFIYIASSLLVSVLLMTGACTKKYEELNTDPKIITGDLIDP
                     GLLLTYVQREAIVRGADYGNGTAGSYCGMCVRSDDGPFWEGDMPGEWNFAYEVAINNL
                     SDIIHLINSREDKDNLVNKVAIARIMKAWAVSKLTDTYGDVPYSESCLPKEQAIYSPK
                     YDTQESIYKDLFKELKEAAAQLDDGKASYGRADLFYGGDVAKWKKLANSLRLRLALRV
                     RYADAALAQAQLSDLTEAGLMSDISDDAYILNNLDYPDNQNPRFYRIVNYNTAQESVL
                     THKVMIDILKNDSLHMDPRIKIYADTVKAPWKGGLSPNGYFFPAFKYRGQPTLGFVPV
                     EYKYPWGANSCSEISDFWRVPVVAPALMRCSEVYFALAEAKLAGLLPAAFSGSAQDYY
                     RKGIDASIEWYKWFYNLAAPQIPDLMIKYLHNPALAPGAQWTQADVDKYLNYKKITDQ
                     EVDDFKATPMYSLSGSEEEQLEKIINQKIVALYPDEFEGWTEYRRTGYPKVPIGPDES
                     HLKGVVPRRNSWPYNEKNINSASYNEALARYGGKDNRLTKFWWDKNPNAPHKYIWTAP
                     SMPKAWQ"
     gene            complement(2059..3138)
                     /locus_tag="PK288_04595"
     CDS             complement(2059..3138)
                     /locus_tag="PK288_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPM18230.1"
                     /translation="MQTEDLVIERMLIEPLNIELAEPFGIAIGTKYSIENVLVTLVLK
                     NGISGYGEAAPLEPINGENQSTVLSTLDSCRDFIVGKSVSDYISISRTLKSVFRAQNT
                     ARCAVEMALLDAYTKFLKIPLYRFFGGVTRKIETDYTITIVTPDEARENATALNAKGY
                     RILKTKVGKDLKGDVERLSAIMDGAPGCSVTVDANQGYTPTDAVRFIQELEKRNIRPL
                     LFEQPVLKDDLYGMKYVKEHISVPVAADESVFSGTDAINVVRSGCADVINIKLMKSGI
                     IEALDIAAIARNANIKLMIGCMLETKLGLGCSVHFAAGLGVFNYIDLDPHIDPALEAF
                     DEGPDFAAPWYSLSDDRCGIGVRKK"
     gene            complement(3241..4338)
                     /locus_tag="PK288_04600"
     CDS             complement(3241..4338)
                     /locus_tag="PK288_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HPM18231.1"
                     /translation="MVVLDGEIGNYVISGNKKYSYFAGNNYLGLATHPEIKDAAVRAV
                     KKYGTGFSASRQTTGTSWLHLELEKELSLFKQKDDSVIFASGYLGNRILMEVLQDRYA
                     SVFIDQDSHPSIIFGIPAGKSDIFRYNHCDTADLESKLKENNRGMPLIITDGVFALTG
                     EIAPLDKIYDLATGYNGILIVDDAHSTGILGETGRGTPEHFGLDGAKGIWQSETMSKA
                     LGSYGGFISSDSVLTGQIRESSAIYQASTSLPPAVVAAGTASLKIIRENPGFRTELTE
                     KARFLRNGIISLGFHTCRGITPIIPIFEDGHESAGNLSAFLEQHGIIVPVINYPGLKS
                     NFMLRITVSVLHEYQQIEKLLDILHDWKNRY"
     gene            4624..5409
                     /gene="agaR"
                     /locus_tag="PK288_04605"
     CDS             4624..5409
                     /gene="agaR"
                     /locus_tag="PK288_04605"
                     /inference="COORDINATES: protein motif:HMM:NF040755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor AgaR"
                     /protein_id="HPM18232.1"
                     /translation="MERLSNKSTVSRRARILEELEAKGQVGVTDLSKMFNISEVTIRN
                     DLTHLEKQNMLIRARGGAIKIKYYRMGIDPSITDKQKEYLKEKQRIANAAVRLIEEGD
                     TIVLDSGTTTTEIAKLLGRFRNLTIITNALNIAVILSEYEGINIFMPGGMLRKKSLSL
                     VGVLADENFGRFFCDKLFLGVDGFDTTYGLSTPNSEEAHLNRIMINTAQKVIVVADSS
                     KFRKKRFALIGPITYVNTVITDSGIKAEDRVSLEKNGIEVIVV"
     gene            5460..6323
                     /locus_tag="PK288_04610"
     CDS             5460..6323
                     /locus_tag="PK288_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPM18233.1"
                     /translation="MIKRPWLLFALTTTIFWGIWGALIELPERAGFPATLIYIVWSLT
                     MVPCAIVGLAIIKWKLDRDPRSILLGSIIGLTGAGGQIVLFQALADGPAYLVFPIISL
                     SPAVTVLLSIVFLRETTTKRHWTGIIMALIAIVLLSFQKPDNTIIKGYLWFIFAILVF
                     LAWGIQAFFMKKANFHMKAESIFFYMMIWALLLNPFAYLFTDWSQEINYGFRGPYLAA
                     IVSLLNSIGALTLVYAIRHGKVIIVAPLTNAIAPVITVFISLVIYSVIPNPVIMAGIV
                     IALIAVYLMAE"
     gene            6394..9573
                     /locus_tag="PK288_04615"
     CDS             6394..9573
                     /locus_tag="PK288_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPM18234.1"
                     /translation="MRNIGLYLILTVLAASLPLAAMCQNRGSAKIAVVQASAMPNEDP
                     FMGNYDPTAVYPKMTGNFNNILKLFEQAGEMGADLVCGPEDIQNIGSYGLHVDKKDPV
                     TGKILFNSLALSVPGPFTDQIAQIARKYKMYIIAPLYEDAGDKVFNSALVFDRQGNII
                     GKHRKTLLPVLETWLVSTGDQYEVYETDFATIAIATCLEISYPEIPSTYALKGADIIF
                     NPTMALDNKPGESLSTASMYITRAKDQSVYIAPVVLGTEGTGIIDFNGNVVAEALGRE
                     NTIIMAEIDFSKERTYNSTWWETINGTNNTRAMMMKLRRPELNATLTNPSPPVLERYK
                     DIKLTTGDRERQLEAVKKVDYGPGEPARKSLLSTMGLDVIPYPQEVKPGSGDFAIGES
                     LTIVLDKNPSPADRFAAEELIRDLGRKWNVRAEVGNEGSGQAIILSRRQVPAAVKPQG
                     YQLTASGKRVIIKARTEDGLFYGTQTLLQLISNAGGKLKIPAMTINDWPDILQRAIHY
                     DTKHHQDKASYVKAFIKELASYKVNMLVWEWEDKFAYPSHPEIGAPGAFTMVEMQEFT
                     RYARQYHIQIVPLVQGLGHVSFILKWPQHKHLREIESSNWEFCPLKQGSYDLLYDLWN
                     DAIKATPGSEYIHIGSDETYELGACDQCRAKAMEIGRSGLYQLFINKSALLLQKKGRK
                     VMAWEAPMEWKTGDSPAKGIEPVKGLILTESYDYETSDLKYVREAKSLGHKVYAYDPN
                     PGVVPMMVPYDFEKSESGENRTGSLEKSFRFLSHAAQSGVFDGMICTSWDDDDLHNQM
                     WMMHFVNAAARSWNGKEPSLGEFRETYFNNYYGRRASGIAELFRLINEGVYYYAWTME
                     RNVWHYGEIGKTHLPDLPRGDALEYDPFWNTRYRQKVEQSEDMLGKMERALRIIEDNL
                     HSGAEHPYDFEILRTTAELVRHTCLTYLNLSALEYAIRDAHRNRFVDCNVSLEKLQSA
                     RQIAEGILERREKVFNDLVRTYEETRFPKGFSTPERQFFWQQDRARHFAFRRPDMSFL
                     IYDEQLLDIEGYIEKLKAYIEYFKANSMN"
     gene            9580..10593
                     /locus_tag="PK288_04620"
     CDS             9580..10593
                     /locus_tag="PK288_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPM18235.1"
                     /translation="MQKREIVTRALNFRDMPYVPWHFKFTVEAREKVLNYLADRDIEE
                     YLQNHILELGDDVGFFKHLGDDLYQDVFGVIWDRSIDKDIGNVRGYLLPEPSLKGYAF
                     PDPRDKRYFKDIPGKIEKYGDRFRLYAMGFSLFERAWTMRGMENLLTDFCLNTDFVHE
                     LMESITQYNLEQIDEALKYDIDGIELGDDWGQQRGLIMGYDMWKEFIYPYLKRTYRFI
                     KDRGKYVFIHSCGDVDELFDDLADIGVDCFNPFQPEVMDVRALLKQYHRKLSFWGGLS
                     IQRILPYGSVDDVIRETRELIEAGKEGGYIFAPSHAVEGDAPVENIIAFIETAVSQPG
                     FRV"
     gene            complement(10611..12047)
                     /locus_tag="PK288_04625"
     CDS             complement(10611..12047)
                     /locus_tag="PK288_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18236.1"
                     /translation="MKRRKFFQLTSAGAAGMAIAPSCSSSAQKKDEKDSGIVINEVVP
                     DYRDQIKASFSVQNKKGANDRVVLALIGAGSWGTNLILEAANSGENILIKYICDVDDT
                     RGGRAISELEKIQGFRPIAVRDMRKVFDDKEVDGVFVETPEHWHALATIRACQAGKDV
                     YVEKTVSHSIFEGQQMIKAAMKYERIVQCGTQNRSADYALTARDYIKNGGLGEIVAVH
                     VKELLEGPVPFNVKEDSPAPDTIDWDMWLGPAPKVPYNVSRNKSWIYYWDYSGGPVTS
                     GGSIHQLDMARLILDNPGFPKSAYCAGGRYFFNDMRDIPDYQMTTFDYGNFVLTLETG
                     ECTPYFQKEGPEVRFGTIYPKWMQNATRIDIYGTERMMIAGRMGGGWQVFDKDEKLVA
                     QDKGIFPLKAHILNYIDCIRTRKQPNGNIVEGHNSSVLIHLANLSYRAGNKQLLFSPE
                     YETITNNSEARALARRTYRKGFEINQEV"
     gene            complement(12081..13517)
                     /locus_tag="PK288_04630"
     CDS             complement(12081..13517)
                     /locus_tag="PK288_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18237.1"
                     /translation="MKRRKFFKVTSAGAAGFSLAASCRNITGKGDSRTGNTAEKPEVP
                     PYRAAVKSNFATDRARSAADKVILALIGAGGWGSHVIIETAGLNENVFVKYICDVDDT
                     RGGRAIEEVERIQGARPVRVRDMRRIFDDREVDAVIIATPEHWHGLATIRACQAGKDV
                     YVEKCISHSIIEGQKMVEAAMKYERIVQCGTLNRSARYGLTARDYIRSGELGDVVTVN
                     AMELTDGPVPFVEKEDTGAPSTIDWDLWLGPAPKVPYNVSRNKSWAYYWDYAGGYAFG
                     QGVIHQADMLRMALDDPGFPKSVYCTGGRYLFNDERDVPDYQLAVFDYGNFVMTLQGG
                     EFTPYLAKTNEKIRFGNTWPEWQQNATRIEIYGTRRMMYLGVMGGGWQVFDKDMQLVA
                     SDKDIYPLKDHIRNFLSCVRSRKQPNGNILQGHYSASLIHMANLSYRSGCRQLLFSPD
                     YETILNDETARELGRPKYRKGFELPDIV"
     gene            complement(13539..14348)
                     /locus_tag="PK288_04635"
     CDS             complement(13539..14348)
                     /locus_tag="PK288_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18238.1"
                     /translation="MAFMICPLVLCSENLIYSQPQITAGEQAVAAAALIGSGSESGIK
                     KDNCLNLPGTVLFHEKKAGGDGRELSYYSHGQDEKPGSYRPPLFFREDWKETPAATPV
                     NQNHVANKDLVLSLYGPGCDSIKKSHHDRPDDDPYYVWSGLCTGNWAVTLKNRNNFAD
                     LTGFSKIAWRTKQSGFRELRVILKLSDGTWLVSREGDGMSNDWRISEFNLSDLNWFRM
                     DIDNIIEDEPVTGPDLSKVDEIGFTDLMRGGQSIACSRLDWITVYGKPVAR"
     gene            14586..15635
                     /locus_tag="PK288_04640"
     CDS             14586..15635
                     /locus_tag="PK288_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPM18239.1"
                     /translation="MRRSIFIRNTGAALAYMFFNPSAGGAGSPISQQLAPLLKDQSGK
                     PITSPGEWIRKREMIKRVWSEYLGIMAPNPDPPVIRIMNEDNPEELTRQYIEYESERG
                     IRVKAYLLKPRKHEGRLPAAVALHSTGDNQMRKIAGLETPGIESLGYNLAKKGFIVIC
                     PMCFLWHEKGTKSYEEQVELFRSGHPQSKGMAKMLFDAMRAVDVLEQINEVDKKRIGA
                     LGHSLGAKEAFYLGAFDDRVTAVVSNEGGIGLNSTNWDDPWYLGREIHDFPHQHHEVL
                     GLCAPKAFLLIGGDSSDGRISEPYIDAVKPVYGLYGKPENLMFFNHGEGHGITPEAEK
                     ITYGWMIRHLKEKTD"
     gene            15679..18102
                     /locus_tag="PK288_04645"
     CDS             15679..18102
                     /locus_tag="PK288_04645"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18240.1"
                     /translation="MKNFKFIRFLAILILVTGGCTGHRGAEKAPGDGQHHDHWSVIGP
                     GGGGGVLKPTVSPFNKDFVMTHCDMTGVFVSHNGGMTWKMKNLWNVPDDFEFDPVDSN
                     IVYISTHGFLYSEDRGSGISLLLRSTDRGESWEIIYPEISRAKKTECIQSSRLMPSEI
                     MEGAIDGTIQKVEVDPASNARIYLGIAPLIDYMGGRNAESKPGEVFMVSTFNHGQTWN
                     RPVSLPGRKVMAIFPGAPGDKVIVFSDKVCAHIDTSTGKTISFPLPAEEIIAVEGGKD
                     GEGSSIYLQSPFMAGGKGGMYISSDLGKSWKQINNGLLPDPAGDILPSFRQALAVCES
                     RPEVAYISVDVPKKDASGTYYELYCIYKTENGGIKWDPVLVSSSPGGYLTDNFSGSWM
                     EQSYDPGWGGSPIDMGVAPDDPDVCYAGDNGRGYKTTDGGKSWTQVYSHNNPDGTYSS
                     NGLNVTTCYGIHFDPFDRNHFFICYTDIGLFHTFSGGKSWLHSINGLPYRWANTCYDL
                     KFDPAIKGKVWSVWADAHDLPRTKMFGGRGFGGFVGGVAASEDGGLTWKKSNDGLPEN
                     SVCTNILLDPESPAGSRTLYVSVFDRGIYKSTDDGKTWNLINNGLGNNLFAWQLRQNS
                     NGRLFALFVRGMARKQTIDGSLYFSDDKGSTWTPLPLAEGVNGPHDLMIDPVNPDVMY
                     VSCWPRGDKGEDTGGGVIKTTDGGKSWRRVFDERIRVNSAGMDPENPAVIYINTFQNS
                     AFRSEDGGESWKRIGGYRFKWGQRAIPDIHNPGMLYLTTYGGSVFYGPAKGIPGSTDD
                     ITNMPDGWW"
     gene            complement(18190..21459)
                     /locus_tag="PK288_04650"
     CDS             complement(18190..21459)
                     /locus_tag="PK288_04650"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPM18241.1"
                     /translation="MKKYFNLSGLLSLTVACCIAMSCRQHQVSRLNPSAFAVPPSSSY
                     IHAWWHWLDNAVTREGITKDLEAMKDQGISTATILNVGLFNERDLGVPPVKFATEEWY
                     RMFEWALSEADRLGMTLGVHNCDGWSSSGGPWIDPANSMKRCVWSRSVAEGGKKLEIR
                     LQEPAASHNFYRDICVLAVPASDGINSFQKAAPEIKVNGKAGRTILYDANPLSSEEIR
                     NGSTIDVSFVNDFQMSSLAIHPRVEFAWTSLDEISFTLELKVSKDGKIFKTVREFSGP
                     PLNRTTILETGHQSAKYFRLEFRDLAGVDALDISELELLNENEEPSYHTVIPYHLEKT
                     CTTMAHKLDDFQVEGEDQRFSVPSGSVVNLTGNMDREGNLKWDAPAGKWELLRIGYTT
                     TGAMNAPATSAGRGLECDKMDTAALNLHFRSFPAKLISHAGKYTGNTFEYLFIDSWEC
                     RYQNWTSNFAGEFRKRRKYDIINWMPVICGITIDNPATTERFLQDFQLTIAELIEENY
                     YRHFHRLCMQNGLKSHAEVIYGGTGYPPVDILRSNSYVDVPMFEFWANPDRRTGLVNY
                     RPVEKTSFPMPAYAGALYGKNVVPAEAYTGYANYSEYPWNLKLFGDNAFCSGINQMVL
                     HSYVHQPFDKKPGITLGMFGQSFNRHNPWWSFASQWFTYHARVQYILQQGVTVADILC
                     FTGDRFYLDPENQSDHRLPPGYNIQLCNFDILKNHCRVEDGKLRLDNGLSYDILLLPD
                     DIYMNPVTIERIGELVKAGATVVGPRPGRAPGFLDHVQNDKIIGEISENVWGKAGTGG
                     INENKYGKGHVYAGLKLMDIIDKLGIRPDLSCKVSDGGKLIYIHKKAGPDDIYYIVNQ
                     EDRVCHSELAFRIRGKYPEIWDPQYGKTYRVEDFSESEGRTVINLKFQPRESLFIVFR
                     DKLSSDIPAYKEPEHKYIPADLKGTLEFEDLPDKKPVEINSLKSWITFDDPEIKYYSG
                     KARYSLKFDLPEELIHGKSLYLCVDSIRTPYEVSINGKPAGCSAFPGYRFEVSSLVRE
                     KDNSVVIRVANPYRNRIIGDFIQYGSLKDLWTTSPLSSLPGKDLPLIESGISGPLSFC
                     Y"
     gene            complement(21467..22180)
                     /locus_tag="PK288_04655"
     CDS             complement(21467..22180)
                     /locus_tag="PK288_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18242.1"
                     /translation="MKTRIFILTSIMISAAFMGYSQDNLYFNRSLQDFASKMQISSGT
                     PGYRAGDRDDSAVEGSEYFNGNFANGEIYTIEKKVFTDIPMRYNAYHGQIEIMMPDQT
                     IRILGNIDNIERIVFDSSTYVYRSFAIGGKMIAGFLVQICKGKNQLLRRDYKVFMAGK
                     PSDGIVNEIPPKIVDRPAEYYIDTGTGMPKYFRSSRELSDILGRNSQEINSFVKKEKI
                     NFKKEKDLVKLFLYIDSLN"
     gene            complement(22277..>22598)
                     /locus_tag="PK288_04660"
     CDS             complement(22277..>22598)
                     /locus_tag="PK288_04660"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPM18243.1"
                     /translation="DDVAFVSGKGTVMPQNLQIDAGAWIDISGSLGSPGRKSGLAIIC
                     DPQTPNYPAPWILRQKGSMQNIVYPGRQRVTLPVDSDLVLKYRIIVHSGQTDYLTDKK
                     VMID"
BASE COUNT         6136 a         5307 c         5170 g         5985 t
ORIGIN      
        1 tttgccatgc cgaccacacg tacatggggt ttcaacatta agctcacatt ctaatcaaac
       61 ccttaaaaat gacgattatg aaaaattatt ttatatacat tgcttcgtct cttcttgtat
      121 cagtgctgct gatgactggt gcatgtacga aaaaatatga agaactgaac acggatccga
      181 agatcattac tggcgacctt attgacccgg gattattact cacctatgtg cagagggaag
      241 ccattgtcag gggtgctgat tatggaaacg gaaccgccgg ttcctactgc ggcatgtgcg
      301 taagatctga cgacggaccc ttctgggaag gtgacatgcc gggtgaatgg aattttgcat
      361 atgaggtcgc tataaataac ctgtctgaca ttatccacct tattaacagc agggaagaca
      421 aagacaatct tgtaaataag gtggcaattg caagaataat gaaggcatgg gccgtttcca
      481 aactgaccga cacctatggc gacgtaccgt attcggaatc atgcctgccc aaggaacagg
      541 ccatatacag tcccaaatac gacacgcagg agagcatcta caaggatctt ttcaaggaat
      601 tgaaggaggc cgctgctcag cttgatgacg gcaaggcaag ctatggcaga gcagatctgt
      661 tttacggcgg cgatgttgca aaatggaaga agcttgccaa cagtctgcgg ctgcgtctgg
      721 cactgagggt acggtatgct gatgcggctc tggcccaggc ccagctcagt gatcttaccg
      781 aagccggtct gatgtcggat atttctgatg atgcttacat ccttaacaac ctcgactacc
      841 ccgataacca aaaccccagg ttctacagga tagtgaatta taatacagcc caggaaagtg
      901 ttttgactca taaggtgatg attgacattc tgaagaatga ctcgcttcac atggacccgc
      961 gtatcaaaat atatgccgac actgttaaag ctccttggaa aggaggattg tctccaaacg
     1021 gatatttctt ccccgcattc aagtaccgcg gtcaacccac actgggcttt gtgcccgttg
     1081 aatacaaata tccgtggggg gccaattcat gttccgagat ttcagatttt tggagggttc
     1141 ccgttgttgc cccagcattg atgagatgct ccgaagttta ttttgccctt gccgaagcaa
     1201 agcttgcagg acttcttccg gctgcatttt caggttcagc ccaggattat tacaggaaag
     1261 gaatagatgc ttcaatcgag tggtataaat ggttctacaa tctggccgca ccacagatac
     1321 ccgacctgat gatcaaatat ctccacaatc cggcattggc tccgggagca caatggacac
     1381 aggccgatgt ggacaagtat ctcaattata agaagatcac ggaccaggaa gtagacgact
     1441 tcaaggctac ccctatgtat tccctgtcag gatcagaaga ggagcagctt gaaaaaatca
     1501 tcaaccagaa gattgttgcc ctttatcccg atgaattcga aggctggaca gagtacagga
     1561 gaaccggtta ccccaaagta ccgatcggac ctgacgaatc acatcttaaa ggcgttgtgc
     1621 cccggagaaa ttcctggccg tacaatgaaa agaacataaa cagcgcaagt tacaatgaag
     1681 cactggcaag atatggcggg aaagacaaca gactgaccaa gttctggtgg gataaaaacc
     1741 ccaatgcacc tcataaatat atctggaccg ctccatcaat gccgaaagca tggcagtaaa
     1801 caggtaggtt tttagggtaa ttagttgtaa aaagctcact gggtatgaat ccggtgagct
     1861 tttttattct ttgaaccaat ttgaaagaat actattaaag caattttctt ccatttgtct
     1921 cattgccggt agttttacag ccgggcagga tggcgtacag gcccaacatg cctttaacca
     1981 tcttttattt tctctttctc aggaagaagg atctggcaga actgcaaaac ttttgaaagg
     2041 aatgactttt gcagacaatt atttcttcct cacccctatt ccgcaccggt catctgacaa
     2101 agaataccat ggtgctgcaa aatcgggccc ttcatcaaat gcttccagtg ccggatctat
     2161 atggggatca aggtcaatat aattaaaaac cccgaggccg gcggcaaaat ggaccgaaca
     2221 accaagcccc agttttgtct caagcatgca gcctatcatc agctttatat ttgcattcct
     2281 ggcaatcgca gcaatgtcga gtgcttcaat tatgcctgac ttcatgagtt tgatattgat
     2341 aacatcagcg catcctgaac ggacaacatt aattgcatcg gtgccgctga aaaccgattc
     2401 atcggcagca accggcactg aaatatgttc tttaacatat ttcatcccgt aaagatcatc
     2461 tttcagaaca ggttgttcga aaagcaaagg ccggatattc cttttctcaa gttcctgaat
     2521 aaaccttacg gcatcggttg gtgtatatcc ctggttggca tcgacagtaa cagaacatcc
     2581 gggagctcca tccatgatag cagataacct ctcaacatca ccttttaaat cttttcccac
     2641 ttttgttttc aaaatcctgt atcctttggc attaagcgcg gttgcatttt cccgggcttc
     2701 atccggcgta acgattgtta ttgtataatc agtctcaatt ttacgtgtaa ccccgccgaa
     2761 gaatctgtaa agcggaattt taaggaattt tgtataagca tccagaagag ccatttccac
     2821 tgcacaccgg gcggtattct gtgcccggaa aacgcttttc agggttcttg aaatcgatat
     2881 ataatccgaa acgcttttcc ctacaataaa gtccctgcaa ctgtcaagag ttgataagac
     2941 cgtagattga ttctctccat ttattggctc aaggggagcg gcttctccgt aacctgagat
     3001 tccatttttg agtaccagcg ttacaagtac attctcaatg ctgtattttg taccaatggc
     3061 gatcccgaat ggttctgcca gttcaatatt gagcggctca ataagcatcc tttcaataac
     3121 aagatcttct gtttgcatat tctatttttt aaaaaaatcg attctctcca ccatgccctg
     3181 acaacttttt attcagtatt tcttctcact tttaattgac ccgcctgctg aaaatcctgt
     3241 ttaatatctg tttttccaat catgcaggat atccagaagc ttttcaatct gctgatattc
     3301 atgtaatact gaaaccgtta tccttaacat gaaattggat ttcagtccgg gataatttat
     3361 gacaggaaca attattccat gttgttcaag gaaagccgac aaattgccgg ctgattcatg
     3421 gccgtcttca aaaatcggga ttatcggagt aattccccgg catgtatgaa atccgagtga
     3481 aataatccca ttcctcagga agcgggcctt ttctgtcagt tcagttctga agcccggatt
     3541 ttccctgatg atcttaagcg aagctgttcc ggcagcaaca accgcaggcg gcagtgaagt
     3601 tgatgcctgg tagattgcag agctttcgcg tatctgtccg gtcagtaccg agtctgatga
     3661 aatgaatccc ccgtagcttc caagtgcttt gctcattgtt tccgactgcc agatcccttt
     3721 ggcgccgtcc agtccaaaat gttcaggcgt tcccctgccg gtttctccca gtatccctgt
     3781 tgaatgagca tcgtccacta tcagtatccc attgtagcct gttgcaagat catatatctt
     3841 atccagcggg gcaatttcac cggtcagggc gaaaacgcca tcggtgatga tcaggggcat
     3901 gcccctgtta ttctctttca gttttgactc cagatcagca gtatcacaat gattataccg
     3961 gaaaatatca cttttgccgg caggtattcc gaagattatg ctcggatggg aatcctggtc
     4021 aataaatact gatgcatacc tgtcctgcag gacttccatt aaaatgcggt tcccaagata
     4081 acctgaagca aaaatcaccg aatcgtcctt ctgtttgaaa agtgacagtt ccttttcaag
     4141 ttcaagatgg agccatgaag ttcctgttgt ctgacgtgag gctgaaaaac cggtgccgta
     4201 tttttttaca gctctgacgg cggcatcctt tatttcagga tgagtcgcca ggcccagata
     4261 attgtttccg gcaaagtatg agtatttttt attgccgctg ataacataat ttccgatttc
     4321 accatccaat actaccataa ggacacagga taaagggtat catggttgcc gaaaaacaaa
     4381 acaggatttg atataaatat aataaatcaa gggaattttc aaggttttaa cagtcatttt
     4441 tcaatcaata agtattgttc acaagtctga atcattgttt taatgatctg aaagcagttt
     4501 ttgtttcaaa aaacagttat ttgtgaatta ataaatggac agatcccttg ttgtgagcta
     4561 tttttattta aattgcataa ttcttttcgg tacaaacata tctgactatt aaatatcagt
     4621 aaaatggaaa ggctctcaaa caaatccact gtttcgcgcc gtgccaggat ccttgaagaa
     4681 ctggaagcca aaggccaggt aggggttaca gatctgagca aaatgttcaa tataagtgag
     4741 gtgaccatca gaaatgacct tactcatctt gaaaaacaga acatgcttat aagggccagg
     4801 ggcggtgcaa taaaaatcaa atattaccgc atgggtatag atccctccat aactgataag
     4861 cagaaggaat atctgaaaga aaagcaaagg atagcaaatg ccgcagtcag acttattgaa
     4921 gaaggagata caatagtcct ggattcaggc accaccacaa cggagattgc aaagcttctt
     4981 ggcagattca ggaatctcac aattattacc aatgccctga acatcgcagt tattctgtcg
     5041 gaatatgaag gaataaacat tttcatgcct ggtggaatgc tcagaaaaaa atccctgtct
     5101 ctcgtgggag ttcttgcaga tgaaaacttc ggcaggtttt tttgcgacaa gctctttctc
     5161 ggggttgacg gatttgatac cacatacgga ttatcaacac ccaacagtga agaggctcac
     5221 cttaacagga tcatgataaa taccgcacag aaagtaattg tggttgcaga ctccagcaag
     5281 ttccgtaaga aaaggtttgc actaattggt ccgataacat acgtcaatac ggtgataacc
     5341 gattccggga ttaaagctga agaccgggtc agcctggaga aaaacggcat tgaagtaatc
     5401 gtggtctgat ccctgcacca caacaattat tgttcctttc atattttaaa ctgtgaatca
     5461 tgattaaaag accgtggctg ctttttgcac ttaccaccac tatattctgg ggaatctggg
     5521 gcgcgttgat tgaactgccg gagagagccg gatttcctgc aacactgata tacattgtct
     5581 ggtccctcac catggtaccc tgtgcaattg tcgggcttgc gattatcaaa tggaagctgg
     5641 accgggatcc caggtcaatc ctgctgggaa gtattatcgg gctgacaggt gccggaggtc
     5701 agattgtact gtttcaggca cttgccgatg gccctgccta ccttgttttt cctattattt
     5761 cgctctcacc cgcggttact gttttgttat ccatcgtttt tctcagggaa accaccacaa
     5821 aacgacactg gacagggatc attatggctc ttattgccat tgtccttctg tctttccaga
     5881 aacccgacaa tactatcata aagggttatc tctggttcat ttttgccata ctggtttttc
     5941 tcgcctgggg aatacaggct ttctttatga aaaaagccaa ctttcacatg aaggcggaaa
     6001 gcatattttt ttatatgatg atctgggccc tcctgttgaa tcctttcgca taccttttta
     6061 cggactggag ccaggaaatc aattacggat tcaggggtcc ctaccttgca gcgatagtat
     6121 cattgctcaa ttccatcggt gcactcaccc tcgtgtatgc catcaggcat ggcaaagtga
     6181 taattgttgc tcctctcaca aatgcaatag caccggtaat aacagttttt atctccctgg
     6241 tcatttattc cgttatcccc aacccggtaa tcatggccgg aatagtaatt gcactgattg
     6301 cggtttatct gatggcggaa tgatgctatt tctcaggtat actgacataa ttgaatgaat
     6361 tacaaagaat ttttccaatt ttaaaaataa aaaatgagaa atatcgggtt gtatctcatc
     6421 cttaccgtat tggctgcatc attacccctt gctgcaatgt gtcagaacag gggttcagca
     6481 aagattgccg ttgtgcaggc ttcggcgatg ccgaatgagg atccgttcat gggcaattat
     6541 gatcccactg cggtatatcc caaaatgacc ggtaatttca acaatatcct caagctgttt
     6601 gaacaggccg gggaaatggg ggcagatctg gtttgcggac ctgaagatat ccagaacatc
     6661 ggatcatacg gcctccatgt cgacaagaaa gatcccgtca cagggaagat tcttttcaat
     6721 tcacttgcac tcagtgttcc gggacctttt acggatcaga tcgctcagat tgcccggaaa
     6781 tacaaaatgt atattattgc tccgctttac gaagatgccg gcgacaaagt gtttaactct
     6841 gcccttgtgt tcgaccgcca gggaaatatc atcggaaagc atcgcaagac ccttctgccg
     6901 gtactggaaa catggctggt atctaccggt gatcagtatg aagtgtatga aactgatttt
     6961 gcaactattg ctattgccac ttgtctggaa atttcatatc cggaaatccc ctcaacctat
     7021 gcgctgaaag gtgccgacat tattttcaat ccgacgatgg ctctcgacaa caagccggga
     7081 gagagcctca gtacagcatc gatgtatatc acaagagcta aggatcaatc ggtttatatt
     7141 gccccggttg tactgggtac cgaaggtacc ggtataatcg atttcaacgg caatgttgtt
     7201 gccgaagccc ttggcaggga gaataccata ataatggctg agattgattt ctcaaaggaa
     7261 agaacttata acagcacctg gtgggaaaca ataaacggga caaacaatac cagggccatg
     7321 atgatgaaat tgcggcggcc ggagctgaac gctaccctta ccaatccctc tcctcctgta
     7381 ctggaaaggt acaaggatat taaacttaca accggtgaca gggaacgaca gcttgaggcg
     7441 gtaaaaaagg tggattacgg gcccggagaa cctgcccgga aaagccttct ttcaaccatg
     7501 ggtctggatg taattccata tccgcaggag gtcaaaccgg gcagcgggga ttttgcaata
     7561 ggggaaagcc tcaccatcgt tcttgataaa aatccttcac ctgccgacag gtttgcagct
     7621 gaagaactga taagggatct tggccggaaa tggaacgtca gggcagaagt tggaaacgaa
     7681 ggcagcggac aggcaattat tttatcccgg cgtcaggtgc cggcagccgt taagccgcag
     7741 ggatatcagc ttaccgcatc aggaaaaagg gtaataatca aagcccgcac tgaggatggt
     7801 cttttctatg gtacacaaac acttctgcag ttaataagca atgcaggcgg taaactgaag
     7861 ataccggcca tgacaataaa tgactggccc gatatcctgc agagggcaat ccattatgat
     7921 accaagcatc accaggataa ggcatcctat gtaaaagctt tcataaaaga actggcttcc
     7981 tacaaggtaa acatgctggt atgggaatgg gaagacaagt ttgcctatcc aagtcatccc
     8041 gaaatagggg cacccggagc attcaccatg gtagagatgc aggagttcac cagatatgcc
     8101 cgacaatatc atattcagat tgttccgctt gtccagggac tcggacatgt aagctttatc
     8161 ctgaaatggc cgcagcataa acatctcagg gaaatcgaat catctaactg ggaattctgc
     8221 cctctgaagc aaggaagtta cgatctgctt tatgacctct ggaacgatgc aataaaagcc
     8281 actcccggct cggaatatat tcatatcggt tcggatgaga catacgaact gggcgcatgt
     8341 gatcagtgca gggcaaaagc aatggagatc ggccgaagcg gattatacca gttattcata
     8401 aacaaatcag ccctcttact tcagaaaaag gggcggaaag tgatggcctg ggaggctccc
     8461 atggagtgga aaacaggaga ttcgccagca aaaggaatag aaccggttaa gggactcatt
     8521 ctcacagaaa gttatgatta tgagacttcg gatctgaaat atgtaaggga ggcaaaatca
     8581 ctcggacata aggtgtatgc ctatgatccc aatccgggtg ttgtccccat gatggtgccc
     8641 tacgattttg aaaaatcgga aagcggcgaa aacaggacag gaagcctcga gaaatcattc
     8701 agattcctct cccatgcggc acaatcgggt gtttttgacg ggatgatctg cacatcatgg
     8761 gatgatgacg atctgcataa ccagatgtgg atgatgcatt ttgtcaatgc agctgccaga
     8821 tcgtggaacg gaaaggaacc atcgctcggg gaattcaggg aaacatactt taataactat
     8881 tacggacgcc gggcatccgg catcgctgaa ctgtttcgtc ttattaacga aggtgtttac
     8941 tactatgcct ggaccatgga gcgtaatgtc tggcactatg gtgagatagg caagacacat
     9001 ctgcccgatc tcccccgcgg tgatgccctg gaatatgatc ccttctggaa cacgcgatac
     9061 aggcagaagg tggagcagtc tgaagatatg cttgggaaaa tggaaagggc attacggatc
     9121 attgaggaca accttcattc tggtgcggag catccctatg attttgaaat attaagaacc
     9181 accgcggaac ttgtcaggca tacctgcctg acctatctca acctttctgc actcgaatat
     9241 gcaatcaggg atgcccacag gaacaggttc gtcgattgca acgtgtcact tgagaagctg
     9301 caatcagcca ggcagatcgc cgaaggcata cttgaaagaa gagaaaaagt tttcaatgat
     9361 ctggtgagga cctacgagga aacgagattc cctaaaggct tttccactcc tgagaggcaa
     9421 tttttctggc agcaggacag ggcaaggcac tttgcattcc gccggcccga tatgtccttt
     9481 cttatttacg atgaacagtt gcttgacatt gaaggataca ttgaaaaatt aaaagcctat
     9541 atcgaatact tcaaggcgaa cagcatgaat taaacctaaa tgcagaaaag ggaaatagtc
     9601 acaagggccc tgaatttccg cgatatgccc tatgtgccct ggcatttcaa atttactgtc
     9661 gaagcaaggg aaaaggtact taattacctt gctgacaggg acattgaaga atacctgcag
     9721 aatcacattc tggaactggg tgatgatgtg ggtttcttta aacacctggg agatgatctg
     9781 taccaggatg ttttcggagt gatatgggac aggagcattg acaaggatat tggtaatgtg
     9841 agggggtatc ttttgcctga accttcactt aaaggctatg cgttccccga tccgcgtgac
     9901 aagagatatt ttaaagatat tcccggaaaa atagaaaaat acggagacag gttcaggctt
     9961 tatgccatgg gattttccct ttttgaaagg gcatggacca tgaggggaat ggaaaatctg
    10021 ctgacggatt tctgcctcaa cacggatttt gtccatgagc ttatggaatc aatcacacaa
    10081 tacaatctcg aacagattga tgaagcactg aaatatgaca tagacggcat agaactgggc
    10141 gacgactggg gtcagcagag aggtctcatc atgggatacg atatgtggaa ggaatttatt
    10201 tatccttacc tgaaaagaac atacagattc atcaaggatc gcggcaaata tgttttcatc
    10261 cattcgtgcg gggatgttga tgagcttttc gacgatctgg ctgatatcgg agtggattgt
    10321 ttcaatccat tccagccgga agtgatggat gtaagggcac tattgaaaca atatcataga
    10381 aaattatctt tctggggtgg cttgtcaatc caaaggattc taccctacgg cagtgtagac
    10441 gatgttataa gggaaaccag ggaactgatc gaagctggca aagaaggagg atatatattc
    10501 gctccttcac acgctgttga aggagacgcc ccggttgaaa acattattgc ttttattgaa
    10561 acagcagtgt cacaaccggg attcagagtc tgaaaaagga taaaatattc tcagacttcc
    10621 tggtttattt cgaatccttt cctgtaggtt cgtctggcaa gtgcacgggc ttcactgttg
    10681 ttggtgatag tttcatactc aggcgagaac agcagctgtt tgttacctgc cctgtaggac
    10741 aggtttgcaa gatggatcag aactgaactg ttatgtcctt ccacgatgtt gccatttggc
    10801 tgcttcctgg tccttatgca atcgatgtaa ttcaggatat gcgccttaag gggaaatatt
    10861 cccttatcct gtgcaaccag cttctcgtcc ttatcgaaaa cctgccagcc gccgcccatt
    10921 cttcctgcaa tcatcatcct ttctgttccg tatatatcaa tccgggtagc gttctgcatc
    10981 cacttcggat agattgttcc gaatcttacc tcggggccct ccttctggaa ataaggagtg
    11041 cattcaccgg tttcaagggt gaggacaaaa tttccataat caaatgttgt catctggtaa
    11101 tcagggatgt cacgcatatc attgaaaaaa tacctgccgc cggcacaata tgctgatttt
    11161 ggaaatccgg ggttgtcaag tatcagtcgg gccatatcga gctggtgtat tgaacctccc
    11221 gaggtgacag gtccgcctga gtaatcccag taatatatcc atgacttgtt cctcgataca
    11281 ttatacggta cctttggtgc cggtcccagc cacatatccc agtcaatcgt atcaggtgcg
    11341 ggagaatcct cctttacgtt gaacggaacc gggccctcga gcagctcctt cacatggacg
    11401 gctacaattt cacccaggcc gccgttcttg atataatccc gggccgtaag ggcataatcg
    11461 gcgctcctgt tctgggttcc gcactgcacg atccgctcat acttcatggc tgccttaatc
    11521 atttgctgcc cttcgaaaat tgaatgagag acagtcttct cgacgtaaac atcctttccg
    11581 gcctgacagg cccggatagt tgccagggca tgccagtgtt caggggtctc gacaaagacg
    11641 ccgtcaacct ccttgtcgtc aaacaccttt cgcatatccc tcaccgcaat gggcctgaat
    11701 ccctgaattt tctccagttc agagatggca cgccctcccc gtgtatcatc aacatcgcaa
    11761 atatatttta tcaggatatt ttcacccgaa ttggcggctt ccagaatgag attagtcccc
    11821 catgaaccgg ctccgatcag tgcaagcacc accctgtcat ttgccccttt tttgttctgc
    11881 acagagaaag aggcctttat ctgatcccgg taatcgggaa caacttcatt tatgacaatc
    11941 ccggaatcct tttcatcctt tttctgtgcg gatgaactgc aggagggtgc aattgccatc
    12001 cctgccgccc ctgccgatgt taactgaaaa aattttcttc gtttcatcag aaatttattt
    12061 actttttgtg aaaaattgtc tcaaacgata tcaggcagct caaacccctt cctgtatttc
    12121 ggacgtccga gctcccgggc tgtttcgtca ttgagtatcg tctcataatc aggtgaaaac
    12181 aataattgcc tgcatcccga tctgtatgac aggtttgcca tatgaataag ggatgcgctg
    12241 taatgaccct ggagaatgtt tccgtttggt tgttttcttg agcggacaca gctgagaaaa
    12301 ttccttatat gatccttaag aggataaatg tccttgtcgc tggcgacaag ctgcatgtcc
    12361 ttatcaaaaa cctgccagcc gccccccatc acacccaggt acatcatgcg ccttgtaccg
    12421 taaatctcta tcctggtggc attctgctgc cattcgggcc aggtgtttcc aaaccttatc
    12481 ttttcatttg tctttgcaag atagggagtg aattctcctc cctgcaatgt cattacaaaa
    12541 tttccatagt cgaacactgc aagctggtag tcgggaacat cacgctcgtc attgaacaga
    12601 taacgccccc cggtgcaata aactgacttc gggaaacccg gatcgtccag cgccatcctc
    12661 aacatatctg cctggtgaat aacaccctgc ccgaaggcat aaccgcctgc ataatcccag
    12721 taatatgccc acgatttatt tctcgacaca ttataaggaa ccttcggagc aggaccgagc
    12781 cacagatccc agtcaatcgt tgagggtgcc ccggtgtcct ccttttctac aaacggaacg
    12841 gggccatctg taagttccat tgcattgaca gtgacaacat ccccgagttc tccgctcctt
    12901 atgtaatcgc gggcagtgag gccgtaacgg gcgcttctgt tcagtgtgcc gcactgaacg
    12961 atcctctcgt atttcatggc agcctcaacc attttctgtc cttcaatgat cgaatgggaa
    13021 atacacttct caacataaac atccttcccc gcctggcagg cacggatggt tgccagtcca
    13081 tgccagtgtt caggtgtggc aatgataaca gcatcaactt ccctgtcatc aaagatcctt
    13141 ctcatatccc tgacccgaac cggcctggct ccctggatcc tttcaacctc ctcaatggca
    13201 cggcctcccc tggtatcatc aacatcacat atatatttga caaacacgtt ctcattcaat
    13261 cctgccgtct cgatgatcac atggcttccc catcccccgg cgccgataag ggcaagaatt
    13321 accttatcgg cggcagaccg ggccctgtca gtcgcgaaat tagacttaac cgcagcacgg
    13381 taaggcggca cctccggttt ttcagccgta ttgcccgtcc tgctgtcccc tttgccggta
    13441 atattcctgc atgaggctgc aagtgaaaaa cccgcagctc cggcagatgt gactttaaaa
    13501 aattttcgcc tcttcataaa aggattatta atcagattct atcttgcaac cggttttccg
    13561 taaactgtaa tccagtcgag tctcgaacag gcaatgctct ggcctccccg cataaggtcg
    13621 gtgaaaccta tttcatcaac tttcgacaga tcggggccgg ttaccggttc gtcctcaata
    13681 atattatcaa tatccatcct gaaccagttg aggtccgaaa gattaaactc actgatcctc
    13741 cagtcattcg acatgccatc gccttccctg ctcacgagcc atgttccgtc cgagagcttc
    13801 agtatcaccc tgagttccct gaatccgctc tgtttggttc gccaggctat ttttgaaaat
    13861 cccgtgaggt ccgcgaaatt gttgcgattc ttcagagtca cggcccagtt tcccgtacac
    13921 aggcccgacc acacataata cgggtcgtca tcgggccggt cgtgatggct tttttttatg
    13981 ctgtcacatc ccggtccgta cagggaaagc acaagatcct tgtttgcgac atggttctgg
    14041 ttcacagggg ttgccgcagg ggtctccttc cagtcttccc tgaagaaaag cgggggcctg
    14101 tatgaaccgg gcttttcatc ttgtccgtga ctgtaataag ataattccct gccatctcca
    14161 ccggcttttt tttcatggaa aagaacggtc cctggtaagt tcaggcaatt gtctttttta
    14221 atcccgcttt cagaacctga cccaattaat gcagcggcag ccactgcctg ttctcctgca
    14281 gtgatttgag gctgtgaata aatcaggttt tctgagcaaa gtaccagggg acaaatcata
    14341 aatgccattc gtgccaatat tgtccggcga ctgaaaaaat gaatccggag acagctgcat
    14401 ccggtccggg tctcagaata tattcctttc atctcctgta atatttcagg ttgttaaaaa
    14461 cgatgcgtaa gttattaaat ataaggcaaa gtatttttat aatatatatg aaaaaattaa
    14521 ttttactttc atattcttat cataacaaaa ttataatatt taaattaccg gacccgcact
    14581 ttaaaatgag aagatcaatc tttataagaa acacaggagc tgccctggct tacatgttct
    14641 ttaatccatc tgcaggagga gcaggctcac ccatcagcca gcagcttgct cccctgctga
    14701 aagatcaatc cggcaaaccg atcacgtcgc ccggggaatg gataaggaaa agggaaatga
    14761 taaaaagggt atggtcggaa tatctgggga tcatggctcc caatcccgac cccccggtaa
    14821 taaggatcat gaatgaagac aatcccgaag agcttacaag gcaatatatt gaatatgaaa
    14881 gtgagcgggg catcagggta aaggcttacc ttctgaaacc ccgtaaacat gaaggccggt
    14941 tgcctgctgc ggtggcattg cattccacag gcgataacca gatgaggaaa attgcaggac
    15001 tggagacacc cgggattgag tcgctggggt ataaccttgc aaaaaagggc ttcattgtta
    15061 tctgccccat gtgctttctc tggcatgaaa aaggcacaaa aagctacgag gagcaggttg
    15121 agctgttcag gtcagggcat ccgcaatcga aagggatggc aaagatgctg tttgatgcca
    15181 tgagggcagt ggacgtactg gaacagatca atgaggttga taaaaagagg ataggtgcgc
    15241 tggggcattc cctcggggca aaggaagcat tttaccttgg tgcgtttgat gatcgcgtaa
    15301 ccgccgttgt aagcaacgaa ggaggaatag gcctcaactc caccaactgg gacgatccgt
    15361 ggtatctggg ccgggagata catgatttcc cccatcagca tcatgaggtg ctcggattat
    15421 gcgcaccaaa agctttcctg ctgataggag gcgattcatc cgacggcagg atcagtgaac
    15481 cctatatcga tgccgtgaag ccggtttacg gattgtacgg caaacctgaa aaccttatgt
    15541 tcttcaatca cggagagggc cacgggatca ccccggaagc agaaaagatt acatacggct
    15601 ggatgatcag acatctgaag gaaaaaacag actaaagcaa tttcaccccg gaatcaagtt
    15661 aaacaaaaac cactgataat gaaaaacttc aaattcatca ggttcctggc aatactcatt
    15721 ttggtcaccg gaggctgcac cgggcacagg ggcgctgaaa aagcaccagg agacggacag
    15781 catcatgacc attggagtgt aatcgggccg gggggcggtg gaggggtttt aaaacccacc
    15841 gtcagtccgt tcaataagga ttttgtgatg acccattgcg acatgactgg cgtatttgtg
    15901 tcacataatg gcggtatgac gtggaaaatg aagaatctgt ggaacgtgcc agatgatttt
    15961 gagtttgacc cggtcgattc aaatattgtc tacatttcca cccatggatt tctgtacagt
    16021 gaggacaggg gttcggggat atctttgctg ctgcgatcga cagaccgcgg ggaaagctgg
    16081 gaaataatct atcctgagat atcacgggct aaaaagacag aatgcattca aagcagcagg
    16141 ctgatgccct cggagataat ggaaggagca attgacggca ccatccagaa agtcgaagtc
    16201 gaccctgcca gcaatgcccg catatacctg ggtattgctc ccctgataga ctatatggga
    16261 ggaagaaacg cagaatccaa accgggagaa gtctttatgg tatcaacctt caaccacggg
    16321 caaacatgga acaggccggt ttcactgccg ggccggaagg ttatggccat tttccccgga
    16381 gctcccggtg acaaggttat tgttttttcc gacaaggttt gtgcgcatat tgacacatca
    16441 accgggaaga caatctcttt tcccctgccg gcggaagaaa taattgcagt cgaaggagga
    16501 aaagacggtg agggcagttc catttacctc cagtcaccat tcatggcagg aggaaaagga
    16561 ggcatgtata tcagcagtga tcttggaaaa tcatggaagc agatcaacaa tggcttgttg
    16621 cctgatccgg ccggcgatat tcttccctca ttcaggcagg cccttgctgt gtgtgaatca
    16681 agacctgaag tagcttatat ctcggtcgat gttcccaaaa aagatgcttc aggtacctat
    16741 tatgagctat actgcattta taaaacagaa aatggaggga ttaagtggga tcccgtactt
    16801 gtgtcttcct cccctggtgg ttacctgaca gataatttca gcgggtcatg gatggagcaa
    16861 agctatgatc ccggatgggg tggcagtccg attgatatgg gcgtggcgcc ggatgatccg
    16921 gatgtctgct atgcgggaga taacggccgc ggatataaaa ccaccgatgg cggcaaatca
    16981 tggacccagg tctacagcca taataatccg gacggaacat actcgagcaa cggcctcaat
    17041 gtgacaacat gctatgggat tcatttcgat ccctttgata gaaatcactt tttcatctgc
    17101 tataccgaca ttggattgtt ccatactttc agcggaggta aaagctggct ccactcaatt
    17161 aacggattgc cgtacagatg ggcgaacacc tgctatgatc ttaagtttga tcccgcgatc
    17221 aaaggtaagg tctggtcagt gtgggcagat gcccatgatc ttccaaggac taaaatgttc
    17281 ggcggccgtg gcttcggcgg ttttgttgga ggagtcgcag cttctgaaga cgggggactg
    17341 acatggaaaa agagcaatga cggacttcct gagaattcag tatgcaccaa tatactgctg
    17401 gacccggaat cgccggcagg cagcagaacc ctgtacgtaa gcgtatttga caggggcatc
    17461 tataaatcga cggatgacgg aaaaacctgg aaccttataa acaacgggct ggggaacaac
    17521 ctgtttgcct ggcagctcag gcagaacagc aacggacgtc tgtttgccct gtttgtccgg
    17581 ggaatggcca gaaaacagac aattgacggc tcgctgtatt tcagtgacga taaaggcagc
    17641 acatggaccc ctcttcccct tgcggaaggc gtgaacggtc ctcatgatct catgattgat
    17701 ccggtcaatc ccgatgtaat gtatgtaagc tgctggccac gtggtgataa aggtgaagat
    17761 acaggaggag gagttattaa aaccaccgat ggcggcaaaa gctggcgcag ggtgtttgat
    17821 gaaaggatcc gggtcaactc ggcaggaatg gatccggaga atccagctgt catttacatc
    17881 aatacttttc agaattcggc gttccgctct gaagacggag gagaaagctg gaagcggatc
    17941 gggggataca ggttcaaatg ggggcagcgt gccattcctg acatacacaa cccgggtatg
    18001 ttatacctta caacctatgg cggcagtgta ttctacggtc cggcgaaagg aatccccggc
    18061 agcaccgacg atatcacgaa tatgcccgat ggctggtggt aatagatact tccggattaa
    18121 ggatattgct ttttaccctt gattttaaac tgcaagccaa aaatccgcag cctgaaattg
    18181 caattaccat cagtaacaga aactcagcgg tcctgaaata cccgattcaa tcagaggcag
    18241 atccttgcca ggcagactgc ttaggggcga agtggtccac agatctttta agctgccgta
    18301 ctggataaaa tcaccaatga tcctgttcct gtaaggattg gcgaccctta tcacaacact
    18361 gttgtccttt tcccttacaa gggatgatac ttcaaaccgg taaccgggga aggcggaaca
    18421 tcctgccggt ttgccattga tgcttacctc ataaggggtt ctgatactgt caacacataa
    18481 ataaagtgat ttcccgtgaa tcaattcttc gggaagatca aacttcaatg agtacctggc
    18541 tttgcctgaa taatacttaa tttccggatc gtcgaaagtg atccatgatt taagggaatt
    18601 tatctccacc ggctttttat ccggcaaatc ctcgaattca agcgtcccct ttaaatcagc
    18661 gggtatatat ttgtgttctg gttccttgta tgccggaata tcagatgaaa gcttatccct
    18721 gaatacaatg aatagggatt cgcgcggctg aaacttaaga tttattactg tccgcccttc
    18781 agattcactg aaatcttcta ccctgtaagt ttttccatac tgcggatccc aaatttcagg
    18841 atattttccc ctgatccgga aagcaagctc actgtggcaa actctgtctt cctgatttac
    18901 aatataataa atatcatccg gacctgcttt tttatgaatg taaataagct tcccgccgtc
    18961 gctgacttta caggaaagat cgggccggat tcctagttta tcaataatgt ccataagctt
    19021 caggccagca taaacatgcc ctttaccgta cttattctca tttatcccgc cggttcccgc
    19081 ttttccccaa acattttcag agatctcacc gataatctta tcattctgaa catggtccag
    19141 gaatcctggc gcccggcctg gccttggccc aacgaccgtg gcacctgctt taacgagttc
    19201 cccgattctt tcaattgtca ctggattcat ataaatatca tcgggcaaaa gcagaatatc
    19261 atagcttagt ccgttatcga gacggagttt gccatcctca acccggcaat gatttttaag
    19321 aatatcgaaa ttgcacagct gaatattgta acctggcggc agccgatggt cactctgatt
    19381 ttcaggatcg agataaaacc ggtcgcctgt aaaacacaga atatccgcaa ccgtgacgcc
    19441 ctgctgcagg atgtactgga cccgtgcatg ataggtaaac cactgtgatg caaagctcca
    19501 ccatggattg tgcctgttaa aactctgtcc gaacattccg agagtgattc cgggcttttt
    19561 gtcaaacggc tggtggacat agctgtgaag caccatctga ttgatccccg aacagaaagc
    19621 attgtccccg aataacttaa ggttccatgg atactcactg taattggcat atccggtata
    19681 ggcttcggcg ggtaccacat ttttaccata cagggctccg gcatatgccg gcatgggaaa
    19741 tgaggttttt tcaaccggcc tgtagtttac caggcctgtc ctcctgtcag gattggccca
    19801 gaattcgaac atgggaacat ccacataact gtttgaacga agaatatcaa caggaggata
    19861 tccggtgcct ccgtaaataa cttcagcatg ggatttcagg ccgttctgca tgcagagcct
    19921 gtgaaaatgc cggtagtagt tctcttcgat aagttcggct attgtaagct gaaaatcctg
    19981 caaaaatctt tcggtggtag caggattatc tattgtaata ccgcagataa caggcatcca
    20041 gttgattatg tcgtattttc tccgctttct gaattcaccg gcaaaattcg aggtccagtt
    20101 ctgatatctg cattcccagc tgtcgataaa aagatattca aaggtatttc ctgtatattt
    20161 tcctgcatgg ctgataagct ttgcggggaa actccggaaa tggagattga gggccgctgt
    20221 atccatctta tcgcattcca gtcccctgcc ggcactggtt gccggtgcat tcatggctcc
    20281 cgtcgtggta tatccaatcc tcaacaactc ccatttacct gccggcgcat cccatttcag
    20341 attaccttcc ctgtccatat tgcctgtaag gttaacaacc gaaccggaag ggaccgaaaa
    20401 tcgctgatcc tctccctcca cctgaaaatc atccagctta tgggccattg tggtacaggt
    20461 tttttccaga tggtagggta tcaccgtgtg atatgaaggc tcctcatttt cattaagaag
    20521 ttcaagctcg gaaatatcca gtgcatccac gccagccaga tccctgaatt ccagcctgaa
    20581 atatttcgcc gactgatgac cagtttcgag gatagttgtc ctgtttaggg gaggacctga
    20641 aaattccctg acggttttaa atattttccc gtcctttgat actttaagtt caagtgtaaa
    20701 actgatctcg tcgaggcttg tccacgcaaa ttccaccctg ggatggattg ccagcgatga
    20761 catctgaaaa tcgttaacga atgaaacgtc tattgtggaa ccgtttctta tttcttcgct
    20821 gcttaatgga ttggcatcat aaagaattgt tctgccggct ttcccattca ccttaatttc
    20881 aggtgccgcc ttctgaaaag aattgatccc gtcggaggca ggtaccgcaa gaacgcagat
    20941 atccctgtaa aaattatgac tggcggcagg ttcctgcaat ctgatttcca actttttccc
    21001 tccttcggcc accgatctgc tccaaacaca tcttttcatc gaattggcag gatcaatcca
    21061 tggtcctccg cttgaactcc atccgtcgca gttatgcacg cccagggtca tgccaagcct
    21121 gtcagcttcg gaaagagccc attcaaacat ccggtaccat tcctcagtag caaatttcac
    21181 cggcgggaca ccaagatccc tctcattgaa aagccctaca ttcaggatgg ttgctgtgga
    21241 aataccctga tccttcatcg cttcaagatc cttcgtaata ccttccctgg ttacggcatt
    21301 atcgagccag tgccaccagg catgaatgta actggaagac ggtggaactg cgaatgcgga
    21361 agggttcagt ctgcttacct gatgttgtct gcagctcatt gcaatacagc aggcaacggt
    21421 cagtgacaaa agacctgaca ggttaaaata ctttttcatt tacgggttaa tttagggaat
    21481 caatatataa aaacagtttt accagatctt tttctttttt gaaattgatc ttttcctttt
    21541 tgacaaagga gtttatttcc tggctgttcc tgcccaggat atcagacagt tcacgggaag
    21601 acctgaaata ttttggcatt ccggtacccg tatcgatata atactccgcg ggcctgtcaa
    21661 ctatttttgg ggggatctca tttacgatcc cgtccgatgg ttttcctgcc atgaagactt
    21721 tataatctct tctgagaagc tgatttttgc ctttgcagat ctggacaaga aaaccagcga
    21781 tcattttccc tcctatagcg aaagacctgt aaacataagt ggatgaatca aatacgattc
    21841 tctcaatgtt atctatgttt ccgaggatcc ggatggtctg atcgggcatc atgatctcaa
    21901 tctgtccatg gtatgcatta tacctcattg gaatatctgt aaacactttt ttttcaatag
    21961 tatatatttc tccgttggca aaattcccgt taaagtattc tgaaccttcc acggcggaat
    22021 cgtcccggtc gcctgcacga taccccggcg taccgcttga tatctgcatt ttggaagcga
    22081 aatcctggag cgatctgttg aaatacaggt tatcctggct gtagcccatg aaggcagcag
    22141 atatcataat gctggtcagt atgaagatcc ttgttttcat tgaaggtaat tttagtccga
    22201 tttttgcctg gttattcggc aatacggcag gaacaacatg aagccatttt gtcagtaatc
    22261 cgtaaactat gagtcatcaa tcaatcatca cttttttatc agtcagataa tcagtttgtc
    22321 cggaatgtac tatgatcctg tactttaata cgagatcact gtcgacgggc aaagttaccc
    22381 gctgtcgtcc cggatacaca atattctgca tgctgccctt ctgcctgagg atccatggtg
    22441 caggataatt cggggtctgc ggatcgcata tgatggcaag tccgcttttg cggccggggc
    22501 tgcccaatga tcccgatata tcaatccagg cccctgcatc gatctgaaga ttctgaggca
    22561 ttactgttcc tttaccggaa acaaatgcca catcatca
//
LOCUS       DAOOYG010000033        22580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2124_length_22580_cov_7.019711, whole genome shotgun sequence.
ACCESSION   DAOOYG010000033 DAOOYG010000000
VERSION     DAOOYG010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2124_length_22580_cov_7.019711"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PK288_04665"
     CDS             complement(<1..1184)
                     /locus_tag="PK288_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HPM18244.1"
                     /translation="MKELVSGLMIFLAGFSILRAENETDLRAPLTHVTVFADRALLSH
                     EAPVELASGKTILKLGGLSPYIDPQSIQVKGYGNFVILAVNHQNNYLSNLEDIPEVKS
                     IRNQIETLQLKVEDEKAAIGVLKEKEAFLVANRAILIKETTYSIEQMKNIMDLFTSNM
                     DQVTTLILRKNRLISDYEKQIAVLQQQISDRFGKKNLPSGEISVTVTAEKPAAARISC
                     SYVVTNAGWFPSYDIRVDNITRPVTIYYKANVYQNTGVPWKDVKLSFSNATPWISGNV
                     PRLDTWFIDYYTPLPAAPVTRSIGYSQAKRKTDDAAGLVTLESRAELNAAEAMPVMVQ
                     QQTGETTVTFDVSVPYSILSDGKIQTIEIQRTSSPAEYRYFTIPKLSSGAYLTGNITD
                     WA"
     gene            complement(1515..2930)
                     /locus_tag="PK288_04670"
     CDS             complement(1515..2930)
                     /locus_tag="PK288_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18245.1"
                     /translation="MIVRNLFNEGKPLKRGFLLVISLFLPMIAFGAGSTVPSAGPIRF
                     EFILFALVLAGVALFHKHTFTVAVSGLIVILAFKLIFDPGFHFAEHMFGKTPFLEQLL
                     DKEHREGEWGIMLNLLGLLLGFAILSKVFEESRVPDILPKYLPDDWKGPLLLLLFVFI
                     MSAFLDNIAAALIGGTIAYVVFDKKVHIGYLAAIVAASNAGGAGSVVGDTTTTMMWIG
                     GVSALKVLPAFVASAFAFMVFAWFGSHQQDKFMRIQKDPCTGVVISWRNIAVVALILA
                     GAILSNILYDMPALGVWIAIVTGAFLIKIPWKEVPGAMKGTIFLLCLVMCASMMPVEE
                     LPNASWETAFTLGFLSSVFDNIPLTKLCIDQGHYDWAMLAYAVGFGGSMVWFGSSAGV
                     AITNKFPEGRNVILWLRKGWHIAIAYVVGFFIFLFVMGWNPSDNKEHKIVDCPVPGCP
                     MAGHSGEPDRQDPAGFSGLTE"
     gene            complement(2980..4449)
                     /locus_tag="PK288_04675"
     CDS             complement(2980..4449)
                     /locus_tag="PK288_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPM18246.1"
                     /translation="MFSKLTSCLSKKAVKVFLTLFFSMVPLAVFGAGKEGIMIGPVRI
                     EFIFFGLILLGVALFHKQTFRVAVIGLTVLMVFKLFFDPAFHLYEHFFGTTPFVEQIM
                     DKELRQGEWGIILNLLGLLLGFAVLSKLFEESGVPDKLPRYLPDDWKGPFLLLVFVFI
                     LSSFLDNIAAALIGGTIALVVFRKRVHIGYIVALVAASNAGGSGSVVGDTTTTMMWID
                     GVSAFNVLHAYVAAGVALLFFAWFAARQQDRYQKIQMDPSPDVKVDWKKIINVIMILA
                     GAIISNILYDMPALGVWIAIVAGSLFNRVPWKEVPGALKGTIFLLCLVTCASLMPVEE
                     LPDASWLTALSLGFLSAVFDNIPLTKLCLDQGHYDWGMLAFTVGFGGSMIWFGSSAGV
                     AITNKFPEGRNVVKWIKNGWHVAASYVVGFFVLYLVMGWEPADNKEHKVINCPVPGCP
                     MAQKKVREEAGLNYTDISKGFHYDSDTPGILFVHTHEDN"
     gene            4700..6148
                     /gene="sufB"
                     /locus_tag="PK288_04680"
     CDS             4700..6148
                     /gene="sufB"
                     /locus_tag="PK288_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPM18247.1"
                     /translation="METDQDRIINDVTSGDYKYGFYTDIETEKIPRGLNEDTVRLISE
                     KKEEPEWLLEFRLKSFRQWQNMKMPSWANLKIPEIDYQDIIYYAAPRQKPRVNSLDDI
                     DPELKRTFDRLGIPLEEQKHLSGVAVDAVMDSVSVKTTFRETLAEMGIIFCSFSEAVR
                     EFPELVRRYMGSVVPYTDNFFAALNSAVFSDGSFCYVPKGIRCPMELSTYFRINSANT
                     GQFERTLLVADEESYVSYLEGCTAPMRDENQLHAAVVEIIAEKNAEVKYSTVQNWYPG
                     DKDGNGGIFNFVTKRGLCRGEGSKISWTQVETGSAITWKYPSCILRGDNSTGEFYSVA
                     VTNNHQQADTGTKMIHIGRNTKSTIVSKGISAGYGQNSYRGLVKVLKNAKDARNFSQC
                     DSLLLGDKCGAHTFPYLEVDNQSAIVEHEATTSKIGEDQIFYCNQRGISTEDAIGLIV
                     NGYAREVINKLPMEFAVEAQKLLQISLEGSVG"
     gene            6175..6933
                     /gene="sufC"
                     /locus_tag="PK288_04685"
     CDS             6175..6933
                     /gene="sufC"
                     /locus_tag="PK288_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPM18248.1"
                     /translation="MLVIKNLHAAIEGKKILRGISLEVKPGEIHAIMGPNGSGKSTLA
                     AVLAGRDLAEVTEGSVTYFGRNLLEMAPEDRSREGIFLGFQYPIEIPGVSMVNFLKTA
                     VNEHRKYKGLEPLSASDFLRIMREKKELVDIDSKLANRSVNEGFSGGEKKKNEIFQMA
                     MLEPKLAILDETDSGLDIDALRVVANGVNKLKRPDNSAIVITHYQRLLDYIVPDVVHV
                     LYDGRIVKSAGKELALELEEKGYDWIKKEVDNQN"
     gene            6815..8293
                     /gene="sufD"
                     /locus_tag="PK288_04690"
     CDS             6815..8293
                     /gene="sufD"
                     /locus_tag="PK288_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPM18249.1"
                     /translation="MSYTYYTMAASSNPQARNSRWNSRRRDTTGLRKKLTIRTDMAVN
                     SELTELYSGIYTDNIRRIREGSTSFLNAFRDAAFDRFTTLGIPTRKNENYKYTNLDLF
                     FRHNYKTYFIPDESDFVKAEEFRCDITDLDTHGVVLLNGFYPTINGKIRTLPGEILIG
                     SLNEASRRFPDLVERHYGKYAKSNSDGLVHLNTAMASDGVFVHVPQGSHLNKPVQVIN
                     LVDSTEDVFNQHRNLIIVDDNADLTIIICDHTLSPQKFLTNAVTEIYVGENARFNIIR
                     VQNEHNNACKVTHTFIHQGRNSYSSSNNLTLHGGLVRNNTYHFLGGEGAESSSFGLYL
                     SDKWQHVDNLVSVEHAFPNCTSNQLFKGVLDEMSTGAFNGRIFVDPKAQGTLAYQKNN
                     NILLTDDAKMYTKPQLEIYADDVKCSHGATVGQLDENALFYLQSRGIDKREAKLLLML
                     GFAHEVIQNIGVEALRERIDNLVMQRLKGELSRCASCMVKCG"
     gene            8284..9522
                     /locus_tag="PK288_04695"
     CDS             8284..9522
                     /locus_tag="PK288_04695"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPM18250.1"
                     /translation="MRIIILELKDIAEIRADFPLLSKKVYNKPLVYFDNGATTQKPQC
                     VLDAIQEVFTSYNGNIHRGIHAMSDMTSEAYENARERVRSFINAARKEEVIFTSGTTG
                     SINSVAFSFGERYVGPGDEIIISNLEHHANIVPWQMMCQRKGASLRIIPVNDDGEILI
                     DEFVKLLSPRTRLVSVTQASNALGTIVPVGEIIRIAHSAGIPVLVDGAQGVQHDVVDV
                     SELDCDFYAFSGHKIYGPTGIGILYGKQKWLEELPPYQGGGDMVEKVTFEKTTYNELP
                     FKFEAGTMNFAGAIGLARALDYVLETGRKDIAAREKMLLDYATGKLAEIRPLKIYGNS
                     KNKISTISFLIDGIHQYDTGTILDKQGIAVRTGTHCAQPVMDRFGIDGTVRISMCFYN
                     TLEEIDTLCTGIEKVIRMFS"
     gene            9532..9954
                     /locus_tag="PK288_04700"
     CDS             9532..9954
                     /locus_tag="PK288_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HPM18251.1"
                     /translation="MTINEVQDNIISDFSISDDWMDKYDLLIDIGKNLPAIDPRFKTN
                     DYLIEGCQSKVWLRPDYDGQKISFTADSDAIITRGIVALLIKVLSDRTPDEIIGAELY
                     FIDRIGLRQNLSPTRSNGLLAMIRQIKLYAMAYRARKN"
     gene            10048..10368
                     /locus_tag="PK288_04705"
     CDS             10048..10368
                     /locus_tag="PK288_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HPM18252.1"
                     /translation="MDPSEQLEIEMRIGQVLKSIYDPEIPVNIYDLGLIYDITLDDDH
                     VAHITMTLTAPNCPMAEDLLEEVKFKVAGTRGVKDCEVKLTFKPPWNRNMLSDEAKLE
                     LGLL"
     gene            10365..11321
                     /gene="queG"
                     /locus_tag="PK288_04710"
     CDS             10365..11321
                     /gene="queG"
                     /locus_tag="PK288_04710"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPM18253.1"
                     /translation="MNTGFQGSSLPDLSEKIKEKTRELGFDICGIARSRPLMEYRPIL
                     EEWIGAGMHDRMNFLARDIPKRLDPGFLLKDAKSLVVTGLNYYSDIIQEADDVPLFSR
                     YTYGIDYHEVILSKLNRLLEWIKVLDHDVAGRPVVDSASILEKAWAREAGLGWQGRHS
                     IVISREAGSFFFIGILILNIELDYDEPFEHDLCGDCRLCIDECPTAAINNNRTIDARK
                     CISALTIESRQPLPGNILPLFGRRIYGCDRCQEVCPWNSRVKINNNREFDPDPEIARM
                     TREDWLTLPLEKFSQLFGRSVLRRIRYEKFMENVRAVLNVNN"
     gene            complement(11318..11731)
                     /locus_tag="PK288_04715"
     CDS             complement(11318..11731)
                     /locus_tag="PK288_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPM18254.1"
                     /translation="MQRIIKLSTYSKNGLYDITREVEGVIRESGIRNGIASVYARGAT
                     AAIMIQENWDQSVQNDVITLLNRLIPSGIWEHDRQDDNGDAHLKAGLVGASEVIPVID
                     GKAGLSTWQNVFFCEFDGPRSLREVIVTVIEDRQA"
     gene            complement(12024..12530)
                     /locus_tag="PK288_04720"
     CDS             complement(12024..12530)
                     /locus_tag="PK288_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM18255.1"
                     /translation="MTTFEFNHNLLEMKGNLQRFALSLTADRDTALDLVQDTYLKAIT
                     YKDKFVDYTNLKAWVFTIMKNTFINNYRRNIKENTIIDGTQDLYYLNMPHDKGFASPE
                     SNYAEEEIIRAIDSLGDEFRIPFKMHIDGFKYHEIAEKLGLKIGTVKSRIFFTRQKLM
                     LMLKDYNS"
     gene            12970..13791
                     /locus_tag="PK288_04725"
     CDS             12970..13791
                     /locus_tag="PK288_04725"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPM18256.1"
                     /translation="MDINIKISNGQVLRGFIRNPGGRTKAVIIMVHGLGEHIGRYSFL
                     ADIFMKNEIGFAGADLPGHGRSDGSRGYIRDYGLLYEMLDILQADCRKIFPGIPLFLY
                     GHSLGGGIVLSYLLKKNPPVNGAIVTSPWLKLSDEPSPFKKKLAALAGKILPSVTIPN
                     GIDPQDISNDSNVVKEYNDDPLNHDRISLSLATGAFRAAGGAIREAHLLTKPLLLMHG
                     SDDRICSPEGSTEFAAASRKAELKIWKGGFHELHNDLNRSEVIDYVISWIMNRIS"
     gene            13811..14791
                     /locus_tag="PK288_04730"
     CDS             13811..14791
                     /locus_tag="PK288_04730"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HPM18257.1"
                     /translation="MKLRTTFTIPPSPEKITYHTPVFFLGSCFATSIGKLMELGCLPV
                     MINPFGTVFNPVSVCNTLSAVIQRKRFNSGDLYKTGEKWISFSHYTEFTSGNADSLLK
                     RINDNIEQAHDFLSGSKFLFVTFGTAWVYSFNKTGEIVSNCHKIPQSEFSRRMVSVNE
                     ITDIWSAQLEVINSSFPDLKVIFTISPVRHWKEGAHGNQISKSVLLLAVEELQRHPSK
                     PGYFPAYELVMDDLRDYRFYDTDMLHLSDTATEYIWNEFAKCYIDGNALNIYSEVLKI
                     SKAALHRLRPDSSHPEKFAEEMIERINNVQAKYPFLDLDAQRQYFADLLK"
     gene            complement(14822..15721)
                     /locus_tag="PK288_04735"
     CDS             complement(14822..15721)
                     /locus_tag="PK288_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPM18258.1"
                     /translation="MEEGMNVFSIKDLEDFSGIKAHTIRIWEKRYGILEPERTDSNIR
                     TYSESELKKILNIAYLKKNGYKISRIALLSEDELTREVISLSDRNKTGDRENHPDKIL
                     LSAIKFNDQQFTGVLTPLIRKHGLEEAYLSYLYPLQEKSRILWQTGSLSKAQEQFIRN
                     IIKQVLICEEFNFKITSAKSGNTVAMINTSDLISENNFLFYRYLLRKKGYDVIFSGGT
                     LPASEVIEIYKIRPFNFLIVNAGVHHFSDEKIGYFSNLGKSLFIKKIIFTDIPDPEGR
                     KIPDNILISENPRDFASIIDSIW"
     gene            complement(15868..16239)
                     /locus_tag="PK288_04740"
     CDS             complement(15868..16239)
                     /locus_tag="PK288_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM18259.1"
                     /translation="MKKGLISILLMLSAIALHAQKDTADSLRNYSSRFFFRDNINTNI
                     SIYPVPVKYNTFSIRADKEMKQVRVTNMIGQDIFRVQYQNPQSFTKVMLENPQRGMYL
                     VTIAFSDGSRVVKKIMIEKPD"
     gene            complement(16278..17066)
                     /gene="kdsB"
                     /locus_tag="PK288_04745"
     CDS             complement(16278..17066)
                     /gene="kdsB"
                     /locus_tag="PK288_04745"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPM18260.1"
                     /translation="MKSDSSHRFAGIIPARYASTRFPGKPLADIGGKTMIRRVYEQAS
                     LSLETVYVATDDDRIFKAVRDFGGNVIMTSEAHRSGTDRCAEAISLISGKEGMEFDIV
                     INVQGDEPFILPEQIDLIKSCFDDSSVEIATLVRRAGPGEDIFNPDQPKVVLDSSGNA
                     IYFSRAAIPFIRDSQMAEWTLRHTYYKHIGLYAYKTATLLEITKLPPGELEIAESLEQ
                     NRWLENGYRIRTAVTTLESICIDTPSDLEKAKTHITDFDKFCYL"
     gene            17142..18389
                     /locus_tag="PK288_04750"
     CDS             17142..18389
                     /locus_tag="PK288_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM18261.1"
                     /translation="MKDLQSIKQRFGIIGNYVGLNRAIDIAVQVAPTDLSVLITGESG
                     TGKEVFPQVIHHYSTRKHGQYIAVNCGAIPEGTIDSELFGHEKGAFTGATESRKGYFE
                     VADGGTIFLDEVAELPLSTQVRLLRVLETGEFIRVGSSRVQKTNVRVIAASNVDVPQA
                     IENNRFREDLFYRLNTVPIRIPELRERGEDIFLLFRKFAVDFAEKYRMPAIRLNNEAK
                     NILLSYSWPGNVRQLKNITEQISIIEQEREITPEVLRSYLPDYTGNKLPVLMKRNEDK
                     SFSSEREILYKILFDMKSDMNDLKKLVFDLIKREEADLDFHESDMGTIKNLFGRSGHH
                     ADHYSGTGSKKAQDFSVHHDRSNIQDTEEVVEESLSLTGKEIEMIKKALEKYNGKRKM
                     AAQELGISERTLYRKIKEYGIEL"
     gene            18379..18915
                     /locus_tag="PK288_04755"
     CDS             18379..18915
                     /locus_tag="PK288_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPM18262.1"
                     /translation="MNYKKLIEMRQGIYVIAIILLIPVLFHGCKVTYSFSGASISPEV
                     KTISIQYFQNRASLVQPALSQNLTDALIDKCKSQTSLSFVNGLGDVNFEGEITDYNTR
                     PLTVSADAQAAVNRFTITVRVEFTNAVDPDLSYEQTFSRFEDYDSALDLSQVEDELSR
                     KIIEMIVEDIFNRAFVNW"
     gene            18922..20250
                     /locus_tag="PK288_04760"
     CDS             18922..20250
                     /locus_tag="PK288_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18263.1"
                     /translation="MNKDDFIRMINVNTPIERHLIGEINDIVDAFPYFQTAHLLLLKG
                     LRDTSDVKFENQLKNSAIHVADREVLYNLLNCKAGEARDAEAVRDQTEIQGQSAAIGN
                     EDLPAPSVQLSAEKPETGSEMHETPDAEFKELLPGQPEIETSGKELEYTAVSRKDYSE
                     KGDIEQTVIESAMNSDELIDKYEKSEADESAEGLKKSADYIVTRSIYLSTVTDEEIEN
                     PVFVFEVERENSEERIFYMDPGFSVDERDEYPDHAYAGSRQSDTTGGSRVTSALQHDS
                     GQGLQPDDESVSADTSADKSVRAPDLLTEQKPAREVFPEPDRPKLQMPPAAEYFPGSA
                     GSGMVEVQQPSAESGIVRQTQAELIDKFIMANPRIEPRKEKTETGNEDLARPFTEERG
                     GFITETLAKIYVNQGYYSKAIDIYERLCLKFPEKNSYFATQIEKIREILK"
     gene            20263..20625
                     /gene="secG"
                     /locus_tag="PK288_04765"
     CDS             20263..20625
                     /gene="secG"
                     /locus_tag="PK288_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPM18264.1"
                     /translation="MYIFVSVLVIIACILQVLIVLVQNSKGGGLAANFTSAGQTMGIR
                     KTADFLEKSTWTLAGAILLLCVVATASIPRGSGPERSRIENQIQNVVDPNAIPTLPSA
                     VPPSSSPAGNRDETNPSN"
     gene            20883..21152
                     /locus_tag="PK288_04770"
     CDS             20883..21152
                     /locus_tag="PK288_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPM18265.1"
                     /translation="MKGKVLAGKVLVKPHEAEAKTASGIIIPDSAKEKPRQGTVVMVG
                     APKKDEEMEVKKGDVVLYGKYSGQELSIDNEDYLLISQSDILFIA"
     gene            21185..>22580
                     /gene="groL"
                     /locus_tag="PK288_04775"
     CDS             21185..>22580
                     /gene="groL"
                     /locus_tag="PK288_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573313.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPM18266.1"
                     /translation="MAKEIKYNIDARALLKEGVDQLADAVKVTLGPKGRNVVLEKKFG
                     APHVTKDGVTVAKDIELSDPYRNMGAQMVKEVASKTGDVAGDGTTTATVLAQAIINVG
                     LKNITAGANPMDVKRGIDKAVEHVVEHLKSQAQVVGNDLKKIEQVARISANDDQEIGK
                     LIAEAMDKVKKEGVITVEESKGTSTSVEVVEGMQFDRGYISAYFVTNTEKMEAELENP
                     YILIYDKKISSMKDLLPILETTAQTGRPLLIISEDVEGEALATLVVNRLRGSLRVCAV
                     KAPGFGDRRKEMLEDIAILTGGTVVSEEKGLKLEHATLDMLGTSEKVTVNKENTTIVN
                     GYGDKEMIKARIGQIKQQMTTTTSDYDKEKLQERLAKLSGGVAVLYIGAASEVEMKNK
                     KDRVDDSLHATRAAIEEGIVPGGGVAYIRAISAIEKLKGENDDQTTGIEIVKRAIEDQ
                     LHRQFFFTGDPRRHI"
BASE COUNT         6544 a         4867 c         5298 g         5871 t
ORIGIN      
        1 tttgcccagt cggtgatgtt accggtcagg taagcccctg aagacagctt gggaatggtg
       61 aaatacctgt attcagcggg ggatgaggtt cgctggattt caatcgtctg tatctttcca
      121 tctgagagga tggaatatgg aaccgaaaca tcgaaggtga ccgtcgtttc accggtttgt
      181 tgctgaacca taactggcat tgcttcggca gcattaagtt cggcacgtga ttccagtgtt
      241 accagtccgg ctgcgtcatc tgttttcctt tttgcctggc tgtaaccaat actcctggtt
      301 acgggagcag cgggcaaggg agtgtaatag tcaataaacc acgtgtcgag cctgggaaca
      361 ttgcctgaga tccacggggt ggcgttcgaa aagcttagtt tcacatcctt ccatggcacc
      421 ccggtattct gataaacatt tgctttataa taaatggtta cgggcctggt aatgttgtca
      481 accctgatgt cgtaagacgg gaaccagccg gcgttggtta cgacgtagct gcagcttatc
      541 cttgctgcgg cgggtttttc tgcggtcacc gtaactgaga tctcccctga aggaagattc
      601 ttctttccga accggtcgga aatttgctgc tgcaggactg caatctgttt ctcataatca
      661 ctgatcagcc tgtttttcct gagaattaat gttgtcacct gatccatatt gctggtgaac
      721 agatccatga tgtttttcat ctgttcgatt gaatatgttg tctcctttat aagaattgca
      781 cggttggcaa caagaaaggc ttccttttcc ttaagaaccc ctatggctgc cttttcatcc
      841 tcaactttaa gctggagtgt ctcaatctga ttacggatac tttttacctc cggaatatcc
      901 tcaaggttgc tcaggtaatt attctgatga ttgacggcaa ggataacgaa attgccgtac
      961 ccttttacct ggatactttg cggatcaata taaggagaga gtcctcccag tttcagtatg
     1021 gttttgcccg aagcaagttc aaccggagct tcatggctca gcagggcacg atcggcaaat
     1081 actgtcacat gtgttagcgg cgccctgagg tcggtttcat tctcagctct cagaattgaa
     1141 aaacctgcca agaaaatcat caatccagat acaagttctt tcatccgtaa tctgttttga
     1201 tttgtgaact gcattactgc ccggaaagga aaaaagttcc gcacatcctc gcagttttgc
     1261 gtatgccttt tgctttttaa agcgttatga tttagaaaaa acttgaaaaa catggtagtt
     1321 aaagttaagt tattctgatg acctgataac agcaattatc ggaccgaaaa atccgggatc
     1381 cagggaaaga gcaggatcaa ttcaaatgct gaatccgacc ggattatcgc attcatccgt
     1441 tgatataccg ggcgtgaagc tgaaatccgc agctgttcat gtgagaatga atgagataaa
     1501 cttctggtgc aggtttattc cgtaagaccg ctaaagccag ccggatcctg ccggtcaggt
     1561 tctccggaat gtccggccat gggacaaccg ggcaccgggc aatcgactat cttatgctcc
     1621 ttgttgtcag atgggttcca tcccattacg aaaagaaaaa taaagaatcc caccacatag
     1681 gcaatagcta tgtgccagcc ttttctcagc cacagtatta cattcctgcc ttccgggaac
     1741 ttattggtta tggcaacacc ggctgaagaa ccaaaccaca ccattgagcc cccgaatccg
     1801 acggcatagg caagcattgc ccagtcgtag tgcccctggt caatacacaa cttcgtgagc
     1861 ggaatattat cgaaaaccga tgacaggaaa ccaagtgtaa atgcagtttc ccagcttgca
     1921 ttagggagtt cttcaacggg catcattgat gcacacatca caagacacaa aaggaatatg
     1981 gtgcctttca tggctcccgg aacctctttc cacgggatct taattaaaaa ggccccggtt
     2041 actattgcga tccacacacc gagggcaggc atgtcgtata aaatgttcga aaggatggct
     2101 cctgcaagta tcaaggccac aacggcgata tttctccatg aaataacaac acccgtgcat
     2161 ggatctttct gaattctcat gaatttgtcc tgctgatgag atccgaacca tgcaaatacc
     2221 ataaaggcaa aggctgaggc tacgaatgcc ggaagtactt taagcgcact tacgccgcca
     2281 atccacatca ttgtggtggt ggtgtcgccc acgacactgc ctgctcctcc ggcattgctg
     2341 gcagcaacaa tggcagcaag gtacccgata tgaacctttt tatcgaaaac gacataggct
     2401 atcgttcctc caatcagtgc agccgcaatg ttatcgagaa aagccgacat aatgaatacg
     2461 aacaatagca gtagaagagg gcctttccag tcatcaggaa ggtattttgg aagaatatcc
     2521 ggcaccctcg attcctcgaa aactttggaa agtattgcaa aacccagcaa aaggcccagc
     2581 agattcagca ttattcccca ttcaccttcc ctgtgttcct tgtcaagcag ctgttcaagg
     2641 aacggagttt tgccaaacat atgttcagca aaatgaaagc ccggatcaaa tatcagcttg
     2701 aaagcgagta tcacaatcag gccgcttacg gcaaccgtga aggtatgttt atgaaaaagg
     2761 gcaacccccg caagaacaag agcaaacaag atgaactcaa atcttatggg accggctgat
     2821 ggaacagtgc tgccggcacc aaaggcaatc atcggaagga acaaagaaat aaccagcagg
     2881 aaacctcttt ttaacggttt gccctcattg aacagatttc tgactatcat gcgaatgact
     2941 ttttgaaaac aggcgaagcg caaaagtaat gaattatcgt caattatctt catgagtgtg
     3001 aacaaacagg atccccggag tgtcagaatc gtaatggaat ccttttgaga tgtcagtata
     3061 atttaaaccg gcctcttccc gtactttctt ttgcgccatg ggacagcccg gaaccggaca
     3121 gtttattact ttatgttcct tattgtcagc aggctcccaa cccattacca gataaaggac
     3181 aaagaaaccc acgacgtagg atgctgcaac gtgccagcca ttttttatcc atttgaccac
     3241 attgcggcct tccggaaact tattggtgat ggcaactcct gcggatgatc cgaaccagat
     3301 catcgaaccg ccgaaaccta ccgtaaaggc taacatcccc caatcgtaat gaccctgatc
     3361 gaggcaaagt ttggtgaggg gtatgttgtc aaaaacagca gaaaggaatc caagtgacaa
     3421 agcagttaac catgaggcat cggggagctc ctccaccggc atgagggaag cacaggtgac
     3481 aaggcacagc aggaatatcg tgcctttcag ggctccgggc acttctttcc agggtacgcg
     3541 gttgaataaa gatcctgcaa ctatggcaat ccatacacca agggccggca tatcataaag
     3601 gatattcgaa ataattgcgc cggcaaggat catgattaca ttgattattt ttttccagtc
     3661 gaccttaaca tccgggcttg gatccatttg tattttctgg tatctgtcct gctggcgggc
     3721 cgcgaaccag gcaaagaaga gcagggcaac gccagccgca acataggcat gcagaacgtt
     3781 aaaggccgat accccgtcaa tccacatcat ggtggtggtg gtatcgccga ccacacttcc
     3841 cgatcctcct gcattactgg cagcaaccag tgccacaatg tagccaatgt gaaccctttt
     3901 cctgaataca accagcgcaa tggtgcctcc aatcagcgcc gcagcaatgt tgtcgagaaa
     3961 ggaggaaagg ataaagacaa agaccagcag taggaaagga cctttccagt catcggggag
     4021 ataccgaggc agcttgtcgg gtacgcccga ctcttcaaac aattttgaga gtactgcaaa
     4081 cccaagcagc agtcccagta gattgagaat tattccccac tccccctgac gaagttcttt
     4141 gtccattatc tgctccacaa agggtgtggt tccgaaaaaa tgctcataca gatgaaatgc
     4201 cggatcaaag aacaatttaa aaaccataag tactgttaat ccaataacag ctaccctgaa
     4261 agtctgtttg tgaaacaggg caaccccaag cagaatgagg ccgaagaaaa taaactcgat
     4321 cctgactgga ccgatcataa ttccttcctt gccggcacca aaaactgcca gaggcaccat
     4381 gctaaaaaac agggtcaaaa atacttttac ggctttcttt gacaggcatg aagttagttt
     4441 gctgaacata tgcaacgggt tattaaacgg gaatccttat attgaaaatg tgcgaattta
     4501 taaataattt cagcaaatat cctgatccat accaggatat ttaaaggatc ttcctcatgg
     4561 atcttcacgt tcaaattatg gacggaatcc ctgatcatta gtaatatttt taataatttt
     4621 gcgtgcaatt tggaagagcg gatattaatt ctataacaaa accttttgct gtttgttagt
     4681 ttatgataag agaaaattga tggagacaga tcaggacagg ataataaacg atgttacttc
     4741 gggagattat aagtacgggt tctataccga tattgagacc gaaaagatac cccgtggttt
     4801 aaatgaggat actgtacgtc ttatatcgga gaagaaggag gagcctgaat ggctgcttga
     4861 attccgcctg aaatcatttc gtcagtggca aaacatgaag atgccttcat gggctaacct
     4921 gaagatacct gaaatagact accaggatat tatttactat gctgctccca ggcaaaagcc
     4981 ccgggtgaac agcctcgacg acattgaccc tgaactaaag agaacattcg acaggctcgg
     5041 tatacctctc gaggaacaaa agcatctgtc gggggtcgct gtagatgcag tcatggacag
     5101 tgtttcagta aaaacaacct tccgggaaac gctggccgag atggggataa tattttgttc
     5161 cttcagtgaa gctgtaaggg aattccctga acttgtgaga cgttacatgg gtagcgttgt
     5221 tccatatacc gacaattttt ttgcagcctt aaactcagcc gttttcagcg atggttcatt
     5281 ctgctatgtg cctaaaggga tccgatgccc gatggaattg tcgacctatt tcaggattaa
     5341 ttcggccaat accggtcagt tcgaacggac cttgcttgtg gccgacgaag aaagctatgt
     5401 gagctacctc gaaggatgca cggcaccgat gagggatgaa aaccagcttc atgcggcagt
     5461 tgtcgagatc attgcagaga aaaatgccga ggtcaagtat tcaactgtcc agaactggta
     5521 ccccggagat aaggacggga atggaggcat ctttaatttc gtcaccaaaa gaggtctttg
     5581 caggggtgag ggttcaaaaa tttcatggac acaggttgag acaggttctg caatcacatg
     5641 gaaatacccg tcatgtatcc tcaggggcga caattcaacc ggcgaattct actcggttgc
     5701 ggttacaaac aaccaccagc aggccgatac gggcacgaaa atgatccata taggcagaaa
     5761 taccaaaagt acaatcgtgt ccaaaggtat ttcggcaggc tatgggcaaa acagttacag
     5821 gggcctggtc aaggtactca agaatgcaaa ggatgcaagg aatttttcac agtgtgattc
     5881 cctcctgctg ggcgacaaat gcggagcaca cactttcccc tatcttgagg ttgacaatca
     5941 gagtgcaatt gtggagcacg aagcaacaac ctccaaaata ggggaggacc agatatttta
     6001 ctgcaatcag agaggaatat caaccgaaga cgctattgga cttatagtaa acggatatgc
     6061 aagggaagtg atcaacaagc ttcctatgga atttgcagtt gaagcacaga agcttcttca
     6121 gataagcctc gaaggcagtg taggataatc aaatttgaaa acgttttagt catcatgctc
     6181 gttattaaaa atttacatgc tgccattgaa ggaaagaaaa ttctcagggg aataagtctt
     6241 gaagtaaaac ccggtgagat acatgcgatt atgggtccga acggatcagg gaaaagtacc
     6301 ctggcagcag tactcgctgg aagagatctg gctgaggtca ccgaaggatc agtgacgtat
     6361 ttcggtagaa atctgcttga gatggcgccg gaggaccgtt cgagggaagg gattttcctg
     6421 ggctttcagt atccaattga aatacccggg gtcagcatgg tgaacttcct caagacagct
     6481 gttaatgaac atcgcaagta taaaggactg gagcctcttt ctgcctcgga tttcctgagg
     6541 ataatgcggg agaagaaaga gcttgtcgat atcgattcga aacttgccaa cagatcagtg
     6601 aacgaaggat tctcgggcgg cgagaaaaag aaaaatgaga tctttcagat ggctatgctc
     6661 gaaccaaagc ttgccattct cgatgagacg gattcaggcc tcgatataga tgccctgaga
     6721 gtggttgcaa acggcgttaa caagcttaaa cgccctgaca attcggcaat tgtgataaca
     6781 cattaccagc gcctgcttga ttacatagtg cccgatgtcg tacacgtact atacgatggc
     6841 cgcatcgtca aatccgcagg caaggaactc gcgctggaac tcgaggagaa gggatacgac
     6901 tggattaaga aagaagttga caatcagaac tgatatggca gtaaattcag agcttacgga
     6961 actttattca gggatttaca ctgacaatat ccggaggatc agagagggct ctacatcttt
     7021 cctgaatgct ttcagggatg cagccttcga caggttcacc acgctcggga ttcccacaag
     7081 gaagaatgaa aattataaat acacgaatct cgatctgttt ttccggcata actataaaac
     7141 ctattttatc cccgatgaat ctgattttgt caaggcagaa gaattcaggt gtgatataac
     7201 tgatcttgat actcacgggg tggtgcttct gaacgggttt tatcctacca ttaacggaaa
     7261 gataaggaca cttcccggcg aaatactgat aggaagcctt aatgaagctt ccaggcggtt
     7321 tccggatctg gtcgagaggc attatggaaa atatgcaaaa agcaactccg acggcctggt
     7381 tcatcttaac actgccatgg cctccgacgg agtatttgtt catgtcccac agggatcaca
     7441 ccttaacaag cccgtccagg taataaatct tgtcgattct actgaagatg tttttaacca
     7501 gcaccggaat ctgatcatag tggacgataa tgctgattta accattatta tttgcgatca
     7561 tacattgtcg ccgcagaaat ttcttaccaa cgcagtgacg gaaatttatg tgggagaaaa
     7621 tgcaaggttt aacattatca gggttcaaaa tgaacataat aatgcctgca aagtaaccca
     7681 tacgttcatt caccagggca ggaacagcta ctcgtcatct aacaatctga ccctgcatgg
     7741 aggacttgtc cggaacaaca cttatcattt ccttggaggg gaaggtgcgg agtccagctc
     7801 tttcggcctt tacctttctg ataaatggca gcatgtcgat aacctggtca gcgttgagca
     7861 tgcttttccg aactgcacga gcaaccagct tttcaaaggt gtgctcgacg aaatgtcgac
     7921 aggagctttc aacgggcgga tatttgttga cccgaaagcg cagggtacgc tggcatatca
     7981 gaagaataat aatatactgc ttaccgatga tgccaagatg tataccaaac cccagctgga
     8041 gatttatgcc gatgatgtta agtgcagtca tggggccacg gtgggccagc ttgacgaaaa
     8101 tgccctgttt tacctgcaat cgagggggat cgataaacgc gaggcaaagc ttctcctgat
     8161 gcttggattc gctcatgagg tgatacagaa tatcggggtt gaagccctga gggaacgtat
     8221 tgacaatctt gttatgcagc gtcttaaggg agaactgtca agatgtgcaa gctgcatggt
     8281 taaatgcgga taattatcct ggagttgaaa gatatagcag aaatacgggc cgactttccc
     8341 cttttaagca aaaaggttta caacaagccg cttgtctatt tcgataacgg tgccacaacc
     8401 cagaagcctc agtgcgttct cgatgcgata caggaggtgt ttacaagtta caacgggaat
     8461 attcaccggg gtatccatgc catgagcgac atgacttccg aggcgtatga gaatgcaagg
     8521 gaaagggtca gatcgttcat taatgccgcc aggaaggaag aggtgatatt cacatcggga
     8581 acaaccggat caatcaacag tgttgcattc agtttcggtg aaaggtatgt cggaccgggg
     8641 gatgaaataa tcatcagtaa cctcgaacat catgccaata tcgttccgtg gcagatgatg
     8701 tgccagagga aaggtgcatc gctgcgaatt atcccggtga atgatgacgg tgaaatattg
     8761 atcgatgaat tcgttaaact gctttcgccc aggaccaggc ttgtttcagt aacacaggca
     8821 tcaaatgccc tcggaaccat agtacctgtc ggagagatca tccggattgc ccattctgcc
     8881 gggattccgg tactggttga cggagcccag ggagtgcagc acgatgttgt ggatgtatca
     8941 gaacttgact gcgactttta cgctttttcc ggacacaaga tttacggtcc caccgggatc
     9001 ggtattctct acggcaaaca aaaatggctt gaagaactgc ctccctacca gggaggaggt
     9061 gacatggtgg aaaaggtgac ttttgagaag accacataca atgaactgcc tttcaagttt
     9121 gaagccggaa caatgaactt cgcaggagcc ataggactcg ctagggcact cgattatgtg
     9181 ctggaaacag gaaggaagga tatagctgcc cgtgaaaaaa tgcttcttga ttatgcaacc
     9241 gggaaactag cagaaatcag accgctgaag atttacggca attcaaagaa caaaatctca
     9301 accatttcat tcctgattga tggcattcat cagtatgata caggtaccat tcttgacaag
     9361 caggggattg cagtcaggac cggaacccat tgcgcccagc ctgtcatgga cagattcggg
     9421 attgacggca ccgtacggat ctcgatgtgc ttctacaata ccctggagga aattgatacc
     9481 ctctgtacgg gaattgaaaa ggtgatcagg atgttttcat gagaggagaa gatgacgata
     9541 aatgaggttc aggataatat aatttcagat ttttcgatat ctgatgactg gatggacaag
     9601 tacgacctgc ttatcgatat cggaaaaaac ctgccggcaa tcgatccccg ttttaaaacc
     9661 aacgactacc tgatagaggg ctgccagtcg aaagtgtggc tccgtccgga ctatgacgga
     9721 cagaaaatct cattcaccgc cgacagtgat gcaattatta cacggggtat agtggccctg
     9781 ctaataaagg tcctttcaga tcgtactcct gacgagatta tcggggcaga actttatttc
     9841 atcgacagga taggcctgag acagaacctg tcgccgacac gatcgaacgg actgcttgca
     9901 atgatcaggc agatcaagct ttatgcaatg gcatacaggg caaggaagaa ctaggatcgg
     9961 ttctttaagg acaggatcag ctttcaggca ttaatcccgg gaacaggaaa atttatcaga
    10021 ttcagtaacc agttaaaatc atatattatg gacccttctg aacagcttga aattgaaatg
    10081 cggatcggac aggtactaaa aagtatttat gaccccgaaa tcccggtcaa tatttatgac
    10141 ctcggactta tttacgacat cactcttgac gatgatcatg ttgctcatat caccatgacc
    10201 ctgactgcac caaactgccc catggccgag gatctgcttg aagaggtaaa attcaaggtg
    10261 gcaggtaccc ggggcgtaaa ggactgcgag gtcaagctta cctttaaacc cccgtggaac
    10321 aggaatatgc ttagcgatga ggctaaactt gaacttggat tattatgaat acagggttcc
    10381 agggatcctc tttacctgat ctttcggaaa agattaaaga aaagacccgt gaactgggtt
    10441 ttgatatttg cggaattgca agatcacgtc cactcatgga ataccgtccg atcctcgaag
    10501 agtggattgg cgcaggaatg catgacagga tgaatttcct tgccagggat atcccgaaac
    10561 gccttgatcc gggttttctt ctgaaggatg caaaatcgct ggttgtaacg ggcctgaatt
    10621 actattccga cattatacag gaagcagatg atgttccgct gttttcaaga tatacctacg
    10681 gaattgatta tcatgaagtg atactttcaa aactgaatcg gctcctggag tggataaaag
    10741 tgcttgacca tgatgttgca ggccggcctg tcgtcgattc agcaagtata ctggaaaagg
    10801 catgggcaag ggaagccggc cttggctggc agggacgaca ttccatcgtt atcagtagag
    10861 aagccgggtc gttctttttt atcggcattc tgatcctgaa cattgaactg gactatgatg
    10921 aaccgtttga gcatgatttg tgcggagatt gcagattgtg catcgacgaa tgtcctaccg
    10981 cggcaataaa caataacaga acgatcgatg ccaggaaatg tatttccgca ctgacaatcg
    11041 aaagcaggca gcctctacca ggaaatatcc tgccattatt cggaagacgg atatatggat
    11101 gcgacaggtg ccaggaagtc tgcccctgga acagcagggt aaagatcaat aataatcgtg
    11161 aatttgatcc tgatcccgaa attgcccgaa tgaccaggga ggactggctg acccttccgt
    11221 tggaaaaatt ctcgcagttg tttggcagat ctgtattaag gcggatccgt tacgaaaaat
    11281 tcatggaaaa cgtaagggca gtactgaatg ttaacaatta agcctgcctg tcctctatta
    11341 cagtcacgat aacttccctt aggctgcgtg gtccgtcaaa ttcgcagaaa aacacatttt
    11401 gccaggttga caatcctgct ttcccgtcaa tgacagggat aacttccgaa gcaccaacca
    11461 gaccagcttt cagatgtgca tccccgttgt cgtcctgtcg gtcatgctcc catataccgg
    11521 agggaataag ccggttaaga agtgtaatga cgtcattctg taccgactga tcccagttct
    11581 cctggatcat tattgcagct gtagctccac gtgcatatac tgatgcaatg ccattcctga
    11641 ttccgctttc cctgataacc ccttccacct cccgggttat gtcatacagg ccgtttttac
    11701 tgtatgtgct cagcttaatg attctctgca tcctgaaccc tgtttattcc ataaatataa
    11761 tgaaattaat ccgggttggt caatctggtg agaaaaagat gaggctgtct caaattccat
    11821 tacctcaaaa ttccctgaaa tttccagtcg ttccgaaagg tacctgatct ggcggatccg
    11881 attgcggcaa ccctttcgtt atgctgcaga tgcatcctta cctgcggcat cctttttcac
    11941 ccggaaaagg aacttaatgg tttgagacag cctcccacta aaccaataaa cctaatcaac
    12001 ctgataagaa ccagaaaaaa tcttcagctg ttgtaatctt ttaacatcag cataagtttc
    12061 tgcctggtga aaaagatcct gctctttaca gtaccgatct ttagcccaag cttctctgct
    12121 atttcgtgat acttgaatcc atcaatatgc atcttgaagg gaatcctgaa ttcatcgcca
    12181 agtgaatcga tagccctgat gatctcctcc tcggcgtagt tcgattcagg ggaagcaaac
    12241 cccttgtcat gaggcatgtt cagataatac agatcctgtg tcccgtcaat aattgtattt
    12301 tccttgatat tgcggcgata gttgtttata aaggtgtttt tcatgatcgt gaaaacccac
    12361 gctttcagat tggtgtaatc aacaaatttg tccttgtatg ttatggcttt caaatatgta
    12421 tcctgcacca gatcaagggc cgtgtcacga tcagcagtca ggctcaatgc aaacctttgc
    12481 agattgcctt tcatttcgag aagattatgg ttgaattcga atgttgtcat ggctgcattc
    12541 attttgatgg gacaaatcta gcaaataatt aaagataaac aaatcttaat atctttaaat
    12601 cttattattc cggaattatt caaaagaata caaatacagg acatactgtg tatcagtaga
    12661 ttatagcaaa gttgcagtat ctgttctaaa ttgatgataa attgtgttca cggtaattgg
    12721 tgatttttcc gggatatgac cggcaggatg attttgcagg aaaccgttca gaactcatta
    12781 atttgctccg ttgtacataa tcaactgccg tatcgttccc tttccgtccg gcagttcatt
    12841 tcaaaacgga ttcagcccgt tgatttcatt tataatgttg catctccccg gccatttgct
    12901 ttttcttcga gttcgggccc aatcttaagt tatttcagta tttctttcgt atttttaaaa
    12961 acaaaaagta tggatattaa cattaaaata agcaacggcc aggtactcag gggtttcata
    13021 agaaacccgg gtgggaggac aaaagctgtc ataataatgg ttcatggttt gggtgaacat
    13081 attggcagat attctttcct ggccgatatt ttcatgaaga atgaaattgg atttgccggg
    13141 gctgacctgc ccggacatgg acgttcggac ggctcaagag gatatattcg ggattatgga
    13201 ttattgtacg agatgcttga catcctccag gctgattgca ggaaaatctt tccgggtatt
    13261 ccgttgttcc tgtacgggca cagccttgga ggcgggatag ttctcagcta tctcctgaag
    13321 aagaatcctc cggttaatgg ggcaatagtt acttctccat ggctgaaact ttccgacgaa
    13381 ccttcaccat ttaaaaagaa actcgcagct ctggcgggaa agatccttcc ttccgtgact
    13441 ataccgaacg gaatcgatcc tcaggatatt tcaaacgaca gtaatgtggt aaaggaatat
    13501 aatgatgatc cgctcaacca tgacaggatt tccctgagtc ttgctacagg tgctttcagg
    13561 gcggccggcg gagccataag ggaagcgcat ctgctcacga agccgctttt gcttatgcat
    13621 gggagtgatg acaggatttg ttcacctgaa ggaagtaccg aattcgctgc ggcaagcagg
    13681 aaggcggaat tgaaaatatg gaaaggcgga tttcatgaat tgcataatga tctgaacagg
    13741 tcggaagtta ttgattatgt cataagctgg atcatgaaca ggatttcatg aatatttccc
    13801 tgaaaggaag atgaagctca gaacaacttt taccattcct ccttcaccgg aaaaaatcac
    13861 ttatcatacc ccggtgtttt ttctcggatc atgttttgcc acatcgatcg gaaagctgat
    13921 ggaactgggc tgtttgcccg taatgataaa tccctttgga acggttttta acccggtttc
    13981 tgtttgcaac acactcagcg ctgttattca gcgaaaaaga ttcaattctg gcgatctata
    14041 caaaaccggc gaaaaatgga ttagtttcag tcattatact gaatttactt caggaaatgc
    14101 tgatagcctt ttaaaaagga tcaatgacaa tattgaacag gcccacgatt tcctgtctgg
    14161 ttcaaagttc ctgtttgtga ctttcgggac cgcctgggta tattcgttta acaaaaccgg
    14221 cgaaatagta tcaaactgtc ataaaatccc gcaatcggaa tttagccgga gaatggtttc
    14281 ggtaaatgaa ataaccgata tctggtcggc acaacttgaa gttattaatt catcttttcc
    14341 cgacctgaag gttattttta caataagccc cgtaagacac tggaaagaag gtgcacatgg
    14401 caaccagata agtaaatcgg tattgcttct tgcagtcgaa gaacttcagc gacatccgtc
    14461 aaagccgggt tactttcctg cctatgagct ggttatggac gacctgcggg attatcgttt
    14521 ttatgatacc gatatgcttc acttgtcgga tacagctacc gaatatatat ggaacgaatt
    14581 tgctaagtgc tatattgacg gcaatgcatt gaacatatat tcggaagtac taaaaatctc
    14641 aaaggcagct ttgcacaggc tcaggcctga ctcatcccat ccggaaaagt ttgctgagga
    14701 gatgattgaa cgaattaaca atgtacaggc aaaatatccg tttcttgatc ttgatgccca
    14761 gagacaatat tttgcagacc tcctgaaata aatcattaca ggcaccggaa gagcaattat
    14821 gctaccagat ggagtcaata atactggcaa aatcccgggg attttcagaa attaaaatat
    14881 tgtcgggaat ttttcttcct tccgggtcag gaatatccgt gaaaattatt tttttgatga
    14941 acagcgattt tccaagattg ctgaaatatc ctatcttctc gtctgaaaaa tgatggactc
    15001 cggcattgac aatgagaaag ttgaatggcc tgatcttata tatttcaatt acttccgatg
    15061 ctggcaatgt gcctccgctg aaaatgacat catatccttt tttcctgagc aggtatctgt
    15121 aaaacaggaa attattttcc gatatcagat cagaagtgtt gatcatggcc acagtattgc
    15181 cagattttgc tgaagtgatt ttaaagttga attcttcaca aatgaggacc tgttttataa
    15241 tatttcttat gaattgttcc tgagcctttg acaggcttcc tgtctgccat aggatcctcg
    15301 atttttcctg caaaggatac aggtatgaca agtaggcttc ttccagacca tgttttctga
    15361 tcagtggtgt cagcacccct gtgaattgct gatcattgaa ctttattgct gaaaggagga
    15421 tcttgtctgg atggttttcc cgatcacctg ttttattcct gtcgctgagt gaaattactt
    15481 cccgggtcag ctcgtcctca ctgagaagtg cgatccttga tattttgtat ccgtttttct
    15541 taagataggc aatattcaga atcttcttga gctcggattc actgtatgtc ctaatgttgg
    15601 agtctgtcct ttcaggttcc agaattccat atcttttttc ccatatcctg atggtatgag
    15661 ccttaattcc tgaaaagtcc tcaagatcct ttatcgaaaa aacattcatc ccctcttcca
    15721 tgataattga ataatgaatt aaaccatcgg ataataagat agctccatga taataacaat
    15781 caacccgcag ggttgttatt attttttcaa tattttttta caaaagggac ttcaacgtga
    15841 atccgttgtg cagggaatca gggcatctta gtcgggtttc tcgatcatta ttttttttac
    15901 cactcttgaa ccatcgctga aagctattgt caccaggtac attccccttt ggggattttc
    15961 cagcatgact tttgtgaatg actgggggtt ctgatattgt accctgaaaa tatcctgtcc
    16021 gatcatgttg gtcaccctga cctgtttcat ctccttgtcg gcccgtattg aaaaggtgtt
    16081 gtatttgaca ggcaccgggt aaatacttat attggtattt atattgtccc tgaaaaagaa
    16141 tctggaggaa taattcctga gtgagtcggc agtatccttc tgtgcatgca gtgcaattgc
    16201 agatagcatc agtaagatag aaatcaaccc ctttttcatg gataccattt tgccaaaaac
    16261 agcaagcaaa tttcgtgcta aagataacaa aatttatcga aatccgttat atgggtttta
    16321 gctttttcaa ggtcggaggg tgtatcaata cagatacttt ccagggttgt aacggcggtc
    16381 ctgatcctgt aaccattttc aagccagcgg ttctgttcaa gggattccgc gatttccagt
    16441 tccccgggag ggagtttcgt aatttccagc agggttgcgg ttttgtaggc atacagcccg
    16501 atatgcttat agtatgtatg cctgagggtc cattctgcca tttgtgaatc cctgatgaaa
    16561 ggaattgccg cccggctgaa atatatggca ttccccgatg agtcgagaac cactttcggc
    16621 tgatcgggat tgaatatatc ttcccctggt ccggctcttc tcacaagggt ggcgatttcg
    16681 accgagctgt catcgaagca tgacttgatg aggtcaatct gttcaggaag tataaagggc
    16741 tcatcgccct gtacattgat aacaatgtca aactccatcc cttctttccc ggatataagt
    16801 gatatagctt cggcgcaacg gtcagtgccg ctcctgtgcg cttccgaggt cataatgaca
    16861 ttgcccccga aatccctgac tgctttaaag atcctgtcgt catcagtggc aacataaaca
    16921 gtttcaagag ataaagaagc ctgttcataa accctcctga tcatagtctt cccgcctatg
    16981 tcggccagtg gtttgccggg gaaccgggtc gatgcatatc gtgcaggtat gatacctgca
    17041 aaacgatggc tgctgtcact tttcatgaag ctaatttaag aaattagcat ttcccaatgt
    17101 tttaaaaatg tcttaaattc gcatcattga tgaaacatgg aatgaaagac ttgcaaagca
    17161 tcaagcagag gttcggaatc atcgggaact atgtgggcct taaccgggcc attgatattg
    17221 ctgtgcaggt tgccccgaca gatctttcag tgctgataac cggcgaaagc ggaaccggca
    17281 aggaggtgtt tccgcaggtt attcatcatt acagtacgag gaagcacgga caatacattg
    17341 cagtgaactg cggggccata ccggaaggaa caattgattc agagcttttc ggccatgaaa
    17401 agggcgcctt tacaggggca actgaaagca ggaaaggata ttttgaggtg gctgacggcg
    17461 gtaccatttt cctcgatgaa gtcgcggaac ttcctttatc aacccaggta aggcttctca
    17521 gggtgctcga aacaggcgaa tttatccggg tcggctcatc aagggttcaa aagacgaacg
    17581 tcagggttat tgccgccagt aatgtggacg ttccgcaggc aatagaaaat aacaggttca
    17641 gggaggacct tttctacagg ctgaataccg tccccatcag aatccctgaa ctcagggaaa
    17701 ggggcgagga catctttctg cttttcagga aatttgccgt cgattttgcg gaaaagtaca
    17761 ggatgccggc catacggtta aacaatgaag caaagaatat cctgctcagt tattcatggc
    17821 cgggtaacgt acgacagctt aaaaacataa cggaacagat ttccattata gaacaggaac
    17881 gggaaataac accagaagtg cttaggtctt acctgcccga ttacaccggg aataaacttc
    17941 cggttctgat gaaaaggaat gaagacaagt cgttctcctc tgaacgcgaa attctttata
    18001 agatattgtt cgacatgaaa agtgatatga acgatctgaa aaaactcgtt tttgatctga
    18061 tcaagaggga agaagctgat cttgattttc atgagtcgga catgggaaca attaaaaatc
    18121 tgttcggccg gtccgggcat catgccgatc actattcagg tacaggcagc aaaaaggcac
    18181 aggatttttc agtccaccat gacaggagca atattcagga tacggaggaa gtggtggagg
    18241 aatccctttc actgacggga aaggagattg aaatgataaa aaaggccctt gagaaatata
    18301 acggaaaacg taagatggcc gcacaggaac ttggtatctc cgagcggacc ctgtacagaa
    18361 aaatcaagga atacgggatt gaattataag aaactcatcg agatgcgaca ggggatttac
    18421 gttattgcca taatcctttt aattcccgta ctttttcacg gatgcaaggt tacatattca
    18481 ttttcgggag caagtatttc ccccgaggtt aaaacaatca gcattcaata tttccagaac
    18541 agggcaagcc tggttcagcc ggcattgagt cagaacctga cggatgcgct tatcgacaaa
    18601 tgcaagtcgc agaccagcct gagcttcgtt aacgggctcg gggatgttaa ttttgaaggc
    18661 gagatcacgg attacaatac ccggccgctt acagtttcgg cagatgcgca ggctgccgta
    18721 aacaggttca ccatcactgt aagggtggaa ttcacaaatg cagtcgatcc ggacctgagc
    18781 tatgaacaaa ctttttcgag gtttgaagat tacgacagcg ccctggacct gagccaggtt
    18841 gaagatgaac tttcacggaa gataattgaa atgattgtgg aagatatctt taacagagca
    18901 ttcgtaaact ggtgatgagc catgaacaag gatgacttta ttaggatgat aaacgtcaat
    18961 actcccattg agcgtcattt gatcggtgag atcaatgata tcgtagatgc atttccatat
    19021 tttcagaccg cacacctgtt gttgcttaaa ggtttgaggg atacctcaga cgtaaagttt
    19081 gagaaccagc ttaaaaactc tgccattcat gttgctgaca gggaggtcct ttataatctt
    19141 cttaattgca aagccggtga agcacgtgac gctgaggctg tccgggacca aacagagatt
    19201 cagggtcagt ctgccgccat tgggaacgaa gatttacctg cgccttcagt gcagctgtct
    19261 gccgaaaaac ctgagaccgg ctccgaaatg catgaaactc ctgatgcgga attcaaggaa
    19321 ctccttccgg ggcagcctga aattgaaacc agcggtaagg aattggaata tactgccgtc
    19381 agcaggaaag actattctga aaaaggtgat attgaacaga ctgtgatcga atcagccatg
    19441 aacagtgatg agctgatcga caaatatgag aaaagcgaag ctgatgaatc tgctgaagga
    19501 ctgaaaaaat cggccgatta tatagtaacc cgttctattt acctatcaac cgttaccgac
    19561 gaggaaattg aaaatcctgt ttttgttttt gaagtggaaa gagaaaactc cgaagagaga
    19621 attttctata tggatccggg gttttcagtt gatgaaaggg acgagtatcc tgatcatgca
    19681 tatgccggga gcagacagag tgatacgaca ggcggatccc gggtaacatc agcgttgcaa
    19741 catgattctg gacagggttt gcagccggac gatgaatcag tttcagctga cacatctgcc
    19801 gataaatcag ttagggcgcc ggatttattg acagaacaaa agcctgcccg ggaagttttt
    19861 cctgaacccg acaggccgaa actccaaatg cctccggcag ctgaatactt tcctggatca
    19921 gccggaagcg gtatggttga agtacagcaa ccttcagcag aatcaggcat tgtgcgtcag
    19981 actcaggctg aactgataga taaattcatc atggcaaatc cgaggattga gccgcggaaa
    20041 gaaaaaaccg aaaccggaaa tgaggatctg gcaaggccat ttactgagga aaggggaggc
    20101 ttcataacgg agacacttgc aaaaatctat gtaaatcagg gatattactc caaggcgatc
    20161 gatatttatg aacgattatg tttgaaattt cctgaaaaaa acagttactt tgcaactcaa
    20221 attgaaaaaa taagagagat attaaaataa taagcaatgg ggatctatat atttgtttca
    20281 gttttggtta ttattgcctg catcctgcag gtattgatcg tcctcgtgca gaattcaaaa
    20341 ggagggggcc ttgcagcaaa ctttacttca gcaggtcaga cgatgggaat cagaaaaaca
    20401 gccgactttc ttgaaaaatc aacatggacc ctggcaggtg ccattctcct gctatgtgtg
    20461 gttgcaactg caagcatccc gcgcggatcg ggaccggaac gctccaggat agaaaaccag
    20521 attcagaatg ttgttgatcc gaatgccatt cctactttgc cttcagctgt ccctccttca
    20581 tcttctccgg caggtaaccg ggatgaaacc aatccttcga attaaaaaga cataatattc
    20641 cgacaattgc caggtgtcag tttgtcataa gctgacactt ttttatcaga ttattaacag
    20701 acagaatatc agcaatatac actaccggtg cctgtagcct taatagaaac tgacaatttt
    20761 tgagggaaaa aatgtcggta gagggtcctg aatcatagaa aataatcctt tggcataaat
    20821 tatgctaagg caacaagtcg gaaaatcatg tttaacttta aaactatata gaaatggcac
    20881 aattgaaagg aaaagtttta gccgggaaag tactggttaa acctcatgaa gctgaagcga
    20941 aaaccgcaag cggaattatt attcctgatt ccgcaaagga aaaacctcgt cagggcactg
    21001 ttgttatggt gggcgcaccc aagaaagatg aagaaatgga agttaagaag ggggatgtgg
    21061 tgctttacgg aaaatattcc ggacaggagc tttccattga caatgaggat tatctgctta
    21121 tttcccagtc ggatatctta ttcatagcat aaaaatcata aactaataat aaatactaaa
    21181 cactatggca aaggaaatta aatacaatat tgatgctcgt gccctgctta aggaaggtgt
    21241 ggatcagctg gcagacgccg taaaggtaac attgggtccc aagggccgga acgtggttct
    21301 ggagaagaag ttcggagctc ctcatgtgac aaaggacggg gttaccgttg caaaggacat
    21361 tgaattgtca gatccctatc ggaacatggg tgcacagatg gttaaggaag tagcctcaaa
    21421 gacgggtgat gttgcgggtg acgggacaac aacggccacc gtgcttgcac aggccattat
    21481 taatgtggga ctgaagaata ttactgcagg tgccaatcct atggatgtta aacgcggcat
    21541 cgacaaagct gttgaacatg tagttgaaca tctcaagtca caggcccagg tagttggcaa
    21601 tgaccttaaa aagatcgagc aggttgcaag aatttcggcc aacgatgatc aggaaatcgg
    21661 taaactgatt gcagaagcaa tggataaggt taaaaaagaa ggcgttatta cagttgaaga
    21721 atcaaagggt accagcactt cggttgaggt tgtggaagga atgcagtttg atcgcggtta
    21781 catctcagca tactttgtta caaataccga aaagatggaa gccgaacttg aaaacccata
    21841 cattcttata tatgacaaga agatttcctc aatgaaagac ctgcttccga tccttgagac
    21901 aaccgcacag accggccggc ctcttctcat catttcagaa gatgtggaag gcgaggctct
    21961 tgccaccctt gttgtaaaca ggttgcgggg atcattgaga gtgtgtgccg taaaagctcc
    22021 cggattcggt gacaggagga aagagatgct tgaggatata gccatcctta caggtggtac
    22081 tgttgtttcc gaggaaaagg gactaaaact cgagcatgct acccttgaca tgcttggaac
    22141 atccgagaag gtaacagtca acaaggagaa taccaccatt gtcaacggtt atggtgataa
    22201 ggaaatgatc aaggccagga ttggtcagat caagcagcag atgaccacaa caacttcaga
    22261 ttatgacaag gaaaagctcc aggaacgtct ggccaaactc tcaggcggtg tggccgttct
    22321 ttatattggt gctgcttctg aagttgaaat gaaaaacaag aaagaccgtg ttgatgattc
    22381 acttcacgca accagggccg ccattgaaga gggaatcgtt cccggcggcg gggtggctta
    22441 tattcgtgcc atttctgcaa ttgaaaagct caagggtgaa aatgatgatc agacaaccgg
    22501 aattgaaatc gtaaagcgtg ctattgagga ccaattgcac agacagttct tctttaccgg
    22561 cgatccgcga cgccacattg
//
LOCUS       DAOOYG010000034        22156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2194_length_22156_cov_7.206778, whole genome shotgun sequence.
ACCESSION   DAOOYG010000034 DAOOYG010000000
VERSION     DAOOYG010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22156
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2194_length_22156_cov_7.206778"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PK288_04780"
     CDS             <1..327
                     /locus_tag="PK288_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPM18267.1"
                     /translation="MLWLGGTLRVHGRRVAKLGSQVDIPVTLLRQLDTYSNFSFGKDL
                     LSDDSRSFAFYTFKEGFAFLTDSSAYIYDHQLGKPVVEEGKNPEYAGMLGKAYLQVLY
                     DDYLKR"
     gene            complement(375..1091)
                     /locus_tag="PK288_04785"
     CDS             complement(375..1091)
                     /locus_tag="PK288_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM18268.1"
                     /translation="MEESPTYERIIDLKECKIWQQDNLVLSDVSLSICKGEYLYLVGK
                     VGSGKTSLIKTLNAQLPLREGSGFVAGFDLSGIKPGEIPFLRRKIGVVFQDFQLLIDR
                     TVNDNLEFVLRATGWKDKNLIADRIDQVLGKVGLEMKGYKMPHQLSGGEQQRVVIGRA
                     LLNDPDIILADEPTGNLDPETSEGIIRLLRDISSTGRAVMIATHNHTILKKFPARTIK
                     CENGKVFEVREDEEIELDLL"
     gene            1320..4340
                     /locus_tag="PK288_04790"
     CDS             1320..4340
                     /locus_tag="PK288_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM18269.1"
                     /translation="MNKLKILLVSFIMIMPGMAANAQMSFSENDFDSEFNRGMDLFYK
                     EKYVPAIKYFDSYIRNGDKAEKIRMAEAEYHAALSALRLFNPDAEYRMRTYLKAHPES
                     PRINEAYMSLGDYFYQNKNYRRASEYYGSVDRMELKSSRLPEYSFRYGYSLYMKGDKQ
                     KAQLMFAEIKDIDTEYTPPAIYYYSQIAYEDKMYETALEGFSRLKDDETFGPIVPFYI
                     IQILYLNKDYDEILRTGPDLLQSAPPERAVELYRFIGDANYKKENYREALKYLEQYAA
                     KAKTTEREDKYQLGYCYYKTGNYDRAIQVLSSTSAKSDALSQNIWYVLGDCYLKTGDK
                     KRAQFAFGQASQMNFDNNLKEDALFNFAKLTYETSSSPFGEAIAAFQEYIELYPASDK
                     IEEAYNYLVATFSEIRNYKAAITALDRIRNKDGRLGEAYQRVAFFRGLELLKNMEVEE
                     SITMFNKSLSYGKYNRDLMARATYWRGEAEYRSGKYEGARDDFETFLGIPGAARLGEY
                     ALARYNLAYSQFNLQNYPGALASFRSFESEAPNAKPEILTDTKIRIADCYFITTNYPM
                     AISYYDKVIESGRTDADYAMYQKGFCLGLSNNQKGKIDILTSLISRYPNSSYVPSAIF
                     ERGRAYVALEDFKRGESDFINVIANYQSSAFAPRATIQLGLLYFNLGENEKSIAQFKK
                     VIENYKSTPEARYALTGLKNAYVEINDVEGYFSYVKTIGYGDVSATEKDSLLYASGEN
                     LYMERKYDRAAEVFRNYLREFSYGSFRLNAQFYLAECLMSSRGNRDEALTLYRQVAEV
                     PNNPFLEQALISAADLLYEREEYAEASGYYERLEKTAVTSDMRVAALKGQLRAAYQEG
                     DARKSIEVARKIRNSAGMPEELNREAVFISAKALYSLNQYDEALADFRKVATEIVSIE
                     GAESKYRVAEILFLRGNTDEAEKVVNQFIGQNSPHQYWMAKMFLLLADISIKKGDTLQ
                     ARATLQSLREYYTISDDGILDEVRSKLAGMDEGN"
     gene            4525..6252
                     /locus_tag="PK288_04795"
     CDS             4525..6252
                     /locus_tag="PK288_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18270.1"
                     /translation="MTIKNIISATLSIIILFSPDLAAQKKEQDLRREITLYNPYKPSL
                     ADFKKKSYLPEIDDTVRVIPRFSYNVLSRPYSPEFSISTIKPAALLSDPLPKLYKGYV
                     KLGLGNYATPLAELSISSHRSRQGAIGLYGRHLSSFGKLPLRFFPERVFAGFMDNEAS
                     IYGKKFLGNSILGLSVDYDQKTRYAYGFKTDEPAYLYKPDKKEIKTGYYDVGVKAVFS
                     SANLDSADFSYDFSLSHDYFNYSKYRTFNHTAFSGEMAKSIEKLYAGAILDFDYYKMA
                     DSLHMKPRYIFHANPFIRRSTGQWNFNLGLRFVLARDIESSARPYFYPDIGFGFTIVP
                     EYLGFFTKLDGRLENNDPLNMFSLNPFLRPDTLFRMKNTDHSIAISAGLKGNSGMGGS
                     YVLSASFSHVNDLMLFANIAHPDTLGHIERGNYFVPRTDDAEILNIHGELSGNISERL
                     KFLTSGNFYSYTLAFNSHPWGKPVWNGSFELKYNLRDKILAGAGLSLLGKRYFLVRQS
                     STGWATLSDEVIETPAHFNLSLSAEYRYTRILSFWVKVNNISTRRYYELAFYPSQMFS
                     LMAGFSYSL"
     gene            complement(6236..6529)
                     /locus_tag="PK288_04800"
     CDS             complement(6236..6529)
                     /locus_tag="PK288_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18271.1"
                     /translation="MRVRVRVSVRVSVRVSVRVRVRVSVRVSVRVRVRVRVSVRVSVS
                     VRVSVSVSGRERRRGSGHMNQIIMPEAAVNPRKIQSMPLKMLHAGCSGIRGCS"
     gene            6777..8747
                     /gene="gyrB"
                     /locus_tag="PK288_04805"
     CDS             6777..8747
                     /gene="gyrB"
                     /locus_tag="PK288_04805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPM18272.1"
                     /translation="MSEMEKKLQDSPEYSAESIQVLEGLEAVRKRPAMYIGDIGVKGL
                     HHLVYEVIDNSIDEALAGYCSRIDIVIHEDNSITVKDDGRGIPTGMHEKEHKSALEVV
                     MTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSEQLIATIMREGKIFRQEYRQGKPVT
                     DVETIGGTSKSGTEIKFRPDRSIFQTTDFNFEILSARLRELAFLNKGIVLTLRDMREL
                     VENGNGGKERYMEFHSDLGLVEFVNYLDANRERIIESPIHITFDKTEIPVEIALQYNS
                     SFSENVHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEDSGATAKLKFDINGDDFREG
                     LTAIISVKVAEPQFEGQTKTKLGNSEVMGAVDQAVSTMLTNYLEENPRDAKIIVQKVI
                     LAATARHAARKARELVQRKNEFSGSGLPGKLADCADRDPSRCEIYLVEGDSAGGTAKQ
                     GRDRRFQAILPLRGKILNVEKAMHHRIYESEEIKNIFTALGVNIGTDEDSKALNTSGL
                     RYHKIIIMTDADVDGSHITTLIMTFFFRYMQDLIRGGNLFIATPPLYLVKKGKTERYC
                     WSEEERDAVVSELAQGKESSVSIQRYKGLGEMNAEQLWETTMNPETRTLRQVTIESAA
                     EADHIFSMLMGDEVPPRREFIETHARYARIDA"
     gene            complement(8744..9856)
                     /locus_tag="PK288_04810"
     CDS             complement(8744..9856)
                     /locus_tag="PK288_04810"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPM18273.1"
                     /translation="MNTKVAVRKCTVYDVNEVYTHISRIFGFCGGPGLRNRTVLLKPN
                     ILSDNPPEKCISTHPVVVEAMVRFLRDEGAKVVLGDSPAIHTRGFRAEKSGIENICRE
                     YGIEWIDFSIDAALMKVRDRKIRIASVINEVDLIISLPKLKNHELVYFTGAIKNTLGL
                     VPGFSKAKLHARYQDRESFSRFLVDLNERVTPHFFLMDGIMGMEGRGPGQGTPANTGV
                     LIGSENPLALDIIASEIIGYNPWDIPTNRIALARAVWLGSPDDIVYAGPPIESIIKKD
                     FRRIIIERNTNISIRFLRNRISFMRKLDRRPVFIHRNCTSCGECIKICPSNALRMHAE
                     KQNHVVLNDAACIRCYCCSEVCKHNAVVIRRKLLGV"
     gene            complement(9914..11350)
                     /gene="dacB"
                     /locus_tag="PK288_04815"
     CDS             complement(9914..11350)
                     /gene="dacB"
                     /locus_tag="PK288_04815"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HPM18274.1"
                     /translation="MYRKLIIIISIHIVLNLNGQEKALDLILSDSSMSGASVSMCILD
                     AGSGNTVFEYNPTQSLMPASTMKLITSLAALELLGPAYTFTTSLGYTGILDKRTGKLE
                     GDIIITGGGDPALGSSYFRDHYGDFINDWIGEIRKAGIREIAGKVIVDDSRYDNEPVP
                     SKWLWEDLGNYYGAGAFGLSVFDNTYEVHFRTGGTGTIPRITRINPPEAATELTNKLR
                     AEGSTDNGYVYAAPYSNTGWISGTIPVNRNDFVLRASIPDPPRLLASIIRNRLDSAGI
                     RIQGGPSTSRIENRVPGDIVIIARTNSPRLDSIIRILNHESVNLYAEHLVRELGRVFM
                     NNGSTASGTGIVLKFMKEAGINTKSVFIEDGSGLSPFNAVSARTLVSLLCYAVNRESY
                     FNQFISSLPRAGKDGTLAGYFKDPVFSSRLRAKSGSLTRTRSYAGYIETMSGRVLAFS
                     IIINNFSGPPRRIVSGIEEILKETILQN"
     gene            complement(11350..12381)
                     /gene="rlmN"
                     /locus_tag="PK288_04820"
     CDS             complement(11350..12381)
                     /gene="rlmN"
                     /locus_tag="PK288_04820"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPM18275.1"
                     /translation="MTKENLFGKTLNELISVTKKLGMPGYSARQMAEWLYGKEIRTIE
                     EMTNISKRMRNLLSAEYEFGLSEPVSVAESRDGTRKYLFRVSGGNYVESAFIPDRERA
                     TICISSQSGCKMGCIFCMTGKQGFQGNLNSNEILNQFRSIPEFRKLTNIVYMGMGEPL
                     DNLHEVIKSLEILTGEWGFGWSPHRITVSTVGISDRIREFLENTRVNLAVSLHSPFED
                     ERRMLVPVQKTNPIKEILTLIRNFDFDNQRRISFEYIVFRGINDTPRHIRELARILDG
                     IRCRINLIRFHPVPGSELESPEPEELIEFRDALTKKGIFTTIRASRGIDIQAACGLLS
                     TLKQNKTDR"
     gene            complement(12501..13283)
                     /gene="dapF"
                     /locus_tag="PK288_04825"
     CDS             complement(12501..13283)
                     /gene="dapF"
                     /locus_tag="PK288_04825"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPM18276.1"
                     /translation="MNMITFNKFQGTGNDFIIIDNRRDIFNPGNHKLIKELCDRRFGI
                     GADGLILINSATAEYDFEMKYFNPDGYEGTMCGNGGRCAAGFAKRNNIAGTSIVFRAS
                     DGIHRAVADAGSVDLKMTDVGNISRIGNNYFINTGSPHYLVFHNDIDSLDVNTEGRKI
                     RYSADFHPDGTNVNFIQPVPGGIYIRTYERGVEYETLSCGTGATASAIAAVLTGQIVS
                     SPVNVRTRGGSLSVKFIVRKEMITDVWLSGPAKFVFEGRVEI"
     gene            13454..14905
                     /locus_tag="PK288_04830"
     CDS             13454..14905
                     /locus_tag="PK288_04830"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPM18277.1"
                     /translation="MKAKNLIGAFAMAVLGAAIALFAYSRINSNSVGAVARDSSGIEL
                     RGADTYLTSLSMQERPVDFTYAAEQTVHAVVHVTTKSMVGPQASNPIMEWFYGDRYSK
                     PREVAGYGSGVIISDDGYIITNNHVIDNADNVEVKLNDNRVFTAEIIGRDPTTDIALL
                     KIKANNLPFIKYGDSDQLKIGEWVLAVGNPFNLTSSVTAGIVSAKGRSLGILDADLRI
                     ESFIQTDAALNPGNSGGALVDTRGLLVGITSAIVSPSGAYAGNSFAIPVTIVRKVVED
                     LKQFGEVQRAIIGVNITNVSQDDAKKLKLDEVRGAKISNVVPNGAAEAAGLKENDIII
                     KFNGVSVASPSELQEQVGKFRPGDRASITYIRNGKENTVPIVLKNIAGNTSVVTREMS
                     GSGTVFGARLESLGSSDREKYNIDYGVKVAELKDGKLKDLGVKKGYIILSINGRKVKN
                     ASEVRSASDNESNLKSIEGIQSDGTFFSYSFRN"
     gene            15040..15900
                     /locus_tag="PK288_04835"
     CDS             15040..15900
                     /locus_tag="PK288_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPM18278.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGKEELISVEEEVELAQRIK
                     KGDRSALEKLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSRFEQEYERTPSPEELAE
                     VLELPKEKVTDTLKVSGRHVSVDAPFIEGEDNSLLDVLTNTDSPVADKLLMDESLSRE
                     IYRALATLTERERDIIRFFFGIGCQEMTLEEIGEKFGLTRERVRQIKEKAIRRLRHTS
                     RSRLLKGYLG"
     gene            16445..18121
                     /locus_tag="PK288_04840"
     CDS             16445..18121
                     /locus_tag="PK288_04840"
                     /inference="COORDINATES: protein motif:HMM:NF025021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM18279.1"
                     /translation="MSEEATLPVLQLGPIYFFYSVPARIKLNDRIDLSFSRSSLITTY
                     RYRLEIPTEGMTGIDDKLLSWSLWTNEDFTAIIIPDLDVLGTYKFTVEYKTGTGTLTR
                     RFEKTFEVYSLSNLSSRARPVKPSQEFPVKKEALTPEVTQAEPGYNYPKTAQASAAAT
                     KEESLTPVPNKRISVAEAPATDNTSVRKDTQSTKQPDISAAPVQRTPINKKIQPSNVS
                     NRKTPQIEKLAFPTSDISLFRLYTATGFIKPVNYSFVDSAANGVVTESDPAIVARVPV
                     IIEKIKVDKPTGAYLVPEKTKAPPTLVAYHPPQVIISDQTITGEATGVEVYTSITNMA
                     DDAGIQSEIKISDTLISHNEPGPGKSEIQMEDLAASVGTIAGKPESDIAEDYLLKLLP
                     PTSGKTAASSFNDMLLKAIEENDTALFRESILNGGGNDLAGINGGNVFHLLNGTIVDE
                     YSVFLLKKNGNSIDKKDRNGNSPLHMAILNRDFEYAKCLINQGADLNLMNKMELAPLH
                     LAAFLNEYRLSAFLMAKGAVADIAGNSGYTPLHIASELNNQTIAEQLLDH"
     gene            18287..18610
                     /locus_tag="PK288_04845"
     CDS             18287..18610
                     /locus_tag="PK288_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18280.1"
                     /translation="MLRFSLPYNNALVAKRQRYTITQAVTVPIAAILSAGTAYLKSEA
                     NKYYSLSKIAETEEQARALYDKTRKLDTSVYVTGSLTLASVYGFIHSTIGKRKVTGKM
                     YKTFN"
     gene            18616..19764
                     /locus_tag="PK288_04850"
     CDS             18616..19764
                     /locus_tag="PK288_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18281.1"
                     /translation="MIHKRLIQSAALFILIILIAGCRREEFSWGIKIGDITYSGNVVF
                     LGERELSSLNEITSDQMVFTDKTGEIEKITDKSILVIGISENTPYGSLRKVNSIHSEG
                     NLLTVRTVPALLPDAIKEGKINLKRKLLEKDFRLKFKADGVKIKDTSKSFEGLAVTLD
                     KLSICDDGACNAVIDGAVGITTNIDIGMDFKSNRLTGIVFATTLEKIDEVTLTSTRAF
                     SGASGKVAAEFIHSPIIIDSLVFVAEVKIHCGFKGTASSAITSGVRQDRTISTAARYL
                     SASWSDDRLAHSENFDYITPLVTDAADLKVITGPEITVFLFGIPVQTIKADAYYSVNA
                     QKDNTPSWKLFSGLDGHVTVNNEILGMGQDHILSMVIQASEIASAGGK"
     gene            complement(19783..>22156)
                     /locus_tag="PK288_04855"
     CDS             complement(19783..>22156)
                     /locus_tag="PK288_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM18282.1"
                     /translation="VINSPPPVPAAPSVGAITQPSCAVSTGSVVITGLPSTGTWVLTR
                     TPGGNTYTGTGTSITISGLAANTYTFTVTNSSGCTSAPSGNVVINVRPATPQSPSVGS
                     VTQPDCARATGSVVLNNLPSEGTWILSRTPDGATMSGTGTTTTVSGLAANTYTFSVTN
                     AAGCVSNPSANVIITTQPETPLPPVTGAITQPDCNTSTGSLVVSGLPSAGIWTLTRNP
                     GGVSTSSSGTSITLHGIPPGNYTYVVSNAAGCTSAPSASVIINASPVTPAAPAAETIV
                     QPGCSTETGSVTLNGLPSAGTWTLVRNPGNISTTGTGSRTTITGLLPNTYTFTVTNES
                     GCTSPVSANVIINPQPLTPVPPNIGSVTHPSCTNAMGSIVLNGLPASGVWTITRAPGE
                     IRYSGTGATTTIHDLPSNTYRFTVTNESGCTSAPSGNVTINPQPPTPAAPVIGNINHP
                     TCNNATGTVALDGLPESGTWTLTVSPGGTTYSGTGNSFSVAGLSSGRYTFTVTNSYGC
                     ISSSSSNAVINSQPPTPSAPTVVSITQPVESRPTGSAVLGGLPASGTWVLTRSPGGNT
                     ITGTGTRSAISGLPAGIYTFTVTNSYGCTSSPSPAVGIYTLKATGPGDKILRHNDTIR
                     IGNSEAGSLSIRIESNAEWTVSENSMWMKAVKEPGTSVIKVTYLDNISVFEKIGALKI
                     HYSLNPEMVLFISQKGRVSRLKESKLEAVSIYPNPASTLLNVDLPGEKFDRIVIAITN
                     KLGNTVFIKNYFRFDPGNKIELNISGFPDGQYFVTISDGEDHKTLTLIKH"
BASE COUNT         6170 a         4929 c         5161 g         5896 t
ORIGIN      
        1 atgctttggc tgggagggac cctcagggtg cacggcagga gagtggcaaa gcttggatcc
       61 caggtcgata ttcctgtaac cctgctccgt cagcttgata cgtatagcaa tttcagtttc
      121 ggtaaggatc ttctgtcaga tgattccaga tcctttgctt tttacacgtt caaggaagga
      181 tttgcattcc ttaccgattc atctgcctat atctatgatc atcagctggg aaaacctgtt
      241 gttgaggagg gaaaaaatcc ggaatatgcc ggcatgctcg gaaaggcata tcttcaggtg
      301 ctgtatgatg attatcttaa aagatagatc cctgtgtttc agccagtgtc aggctctgtt
      361 cccggatctt ttattcataa aagatcaagc tcaatttcct catcttccct gacctcgaac
      421 actttgccgt tttcacattt aatggttctc gccgggaact ttttcaggat ggtatggtta
      481 tgtgttgcga tcattactgc ccggccggtg gaactgatat ccctcagaag cctgattatt
      541 ccttccgagg tttccggatc gagatttccc gttggctcat cggcaagtat tatgtcggga
      601 tcatttagca gtgcacgtcc gatgacaacg cgctgttgtt cacctcccga aagctggtga
      661 ggcattttgt atcccttcat ttcgagacca acctttccca gtacctgatc tatcctgtcg
      721 gctatcagat ttttatcctt ccagccggtt gccctgagga caaattcaag attatcgttt
      781 acggttcggt cgatgagcag ctgaaaatcc tggaatacaa ccccgatctt cctgcgaaga
      841 aaaggtattt ccccgggctt tattcccgaa aggtcgaagc cggctacaaa tccgcttcct
      901 tccctgagcg gaagctgggc attcagcgtc tttatcaggc ttgttttgcc gctgccgacc
      961 tttccaacca ggtacagata ctcccctttg cagatactca ggctcacatc cgataaaacc
     1021 agattgtcct gctgccatat tttacattcc ttcaggtcaa taattctctc gtatgtagga
     1081 gactcttcca tcttgattgt ttggcaacgc aaattagtga aagaattgat aactaacatt
     1141 tatatgttga taaaatccgt aataaagccc tgaatgaggc gttttataat tgtatttttg
     1201 catgtatctg agaaatcagg aaataggttt cagatggctt attatcagta ttttacttat
     1261 cgacctcagg ttcctgtacc ggggatttgc ggaagctgac cggccaaaaa ttcgaagtta
     1321 tgaataaatt gaaaatcctg ctggtatcat ttattatgat catgccgggt atggcggcta
     1381 atgcccagat gtcattttcc gaaaacgatt ttgattctga gttcaacagg ggaatggatt
     1441 tgttttacaa agaaaaatat gttcctgcga ttaaatattt tgactcgtac atcaggaacg
     1501 gagacaaggc agaaaagatt cgcatggccg aggccgaata tcatgcagca ttgtcagccc
     1561 tgagactttt caatcctgat gctgagtaca ggatgcggac ttacctgaaa gctcatcctg
     1621 aaagtccgag gatcaatgaa gcatatatgt cgctcggaga ttatttttat cagaacaaga
     1681 attaccggag ggcctcggaa tactacggat cagtagaccg gatggaactg aaaagttcgc
     1741 ggctgcccga atattcattc cgctacgggt attccctcta tatgaaaggc gataaacaga
     1801 aagcacaact catgtttgcc gaaatcaagg atattgacac ggaatataca ccccctgcaa
     1861 tttattacta ttcccagata gcttacgagg ataaaatgta tgaaaccgca cttgagggtt
     1921 tttcaaggct taaagatgac gaaacctttg gtccgattgt gcccttttat atcatccaga
     1981 ttttatatct caacaaagac tacgacgaaa ttctgagaac cggtccggat ctcctccagt
     2041 cggcaccgcc tgaacgggca gtggagttat accgttttat cggggatgca aattacaaaa
     2101 aggaaaatta cagggaggcg ctgaaatacc ttgagcagta tgctgcaaag gcaaaaacga
     2161 ccgaaaggga agacaaatac cagcttggat actgttatta caaaaccggg aactatgaca
     2221 gggcaatcca ggtgttgtct tcaacctcag caaaatcaga tgccctcagt cagaacatat
     2281 ggtatgtcct gggtgactgc tatctgaaaa cgggcgacaa gaagagggca cagtttgctt
     2341 tcggacaggc ttcgcagatg aattttgaca acaatcttaa agaagatgca ctttttaatt
     2401 ttgccaagct tacctatgag acttcatctt ctcccttcgg tgaagccatc gcagccttcc
     2461 aggaatatat tgaactttat cctgcttccg acaagataga agaagcttat aattatcttg
     2521 ttgctacatt ttcggagatc aggaactata aagccgcaat aactgccctc gacaggatca
     2581 ggaacaagga tggccggctt ggggaggctt accagagagt ggctttcttc cgcgggcttg
     2641 aactattgaa gaatatggaa gtcgaagaat caataaccat gttcaacaaa tcgctttcat
     2701 acgggaaata taaccgtgac ctgatggcaa gagccacata ctggagaggc gaagcagagt
     2761 acaggtcagg caaatatgaa ggggccaggg atgattttga aacctttctg ggcatacccg
     2821 gtgcagcccg cctgggtgaa tatgctctgg caaggtataa tctggcctat tctcagttca
     2881 accttcagaa ttatcccggt gccctcgcca gcttcagaag ctttgaatcg gaggctccaa
     2941 atgccaaacc ggagatactc acagatacga aaatcagaat agctgactgt tactttatta
     3001 cgaccaacta tcccatggca atcagctact atgacaaggt tatagagagt ggcaggaccg
     3061 atgcagacta cgccatgtac cagaaaggat tctgtcttgg gctgagtaat aatcagaagg
     3121 gaaagatcga tatactgacc tcactcattt cacgataccc gaattcatcg tatgtgccca
     3181 gtgcgatttt tgaaaggggc agggcttatg ttgccctgga ggatttcaag cggggagagt
     3241 ctgatttcat taatgtcatt gccaattatc agtccagcgc ttttgcacct cgggcaacca
     3301 tccagcttgg tttgctgtat ttcaaccttg gcgagaatga aaaatccatt gcacagttca
     3361 aaaaagtaat cgagaattac aaatcaacac ccgaggcacg ttatgcactg accggcctta
     3421 aaaatgccta cgttgagata aatgacgtgg aaggttattt ttcatacgtg aagacgatcg
     3481 gttacggtga tgtcagtgca actgaaaagg attcactgct ctatgcctcg ggagagaatc
     3541 tttatatgga aaggaaatat gaccgcgctg ctgaagtttt caggaactat ctgcgtgagt
     3601 tctcatatgg cagtttcagg ctcaacgccc aattctacct ggctgaatgc ctgatgtcgt
     3661 cacgcggcaa cagggatgag gcactgacat tgtacaggca ggtcgcagaa gttcccaaca
     3721 atccgtttct cgaacaggcc ctgatttcag ctgcggattt gctttatgaa agggaggagt
     3781 atgcggaggc ttccggatat tatgaaagac tggaaaagac tgctgttact tccgatatgc
     3841 gggttgcagc cctgaaagga cagcttaggg cagcctacca ggaaggagac gcccggaaaa
     3901 gcatcgaagt ggcacggaaa atcaggaatt ctgccggtat gccggaagaa cttaaccggg
     3961 aagctgtttt tatcagcgcc aaggctcttt acagtctgaa tcagtatgat gaggcactgg
     4021 ccgatttcag aaaggtggca acggaaatag taagtatcga aggtgccgaa tcgaaataca
     4081 gggtggctga aatactcttt ttgagaggca ataccgatga agctgaaaaa gtggttaacc
     4141 agttcatcgg tcagaattca ccacaccagt actggatggc caaaatgttc ctgttacttg
     4201 ccgacatcag cataaaaaaa ggggatacac tgcaagcaag agctacattg caaagcctaa
     4261 gggaatatta caccataagt gatgacggaa tccttgacga ggtcagatca aagctggcag
     4321 ggatggatga aggaaactaa attgctttta tgacgtatct gactgatgca gaaatatgat
     4381 ccgcatgatt gaccagaaac acagaaacga atgaatataa acgctttcag taatgcgtgg
     4441 atccctgccc gacggataag acggacatcc ggacttttaa agaaaaatta tacggatgat
     4501 aacagaatca gaaaacaaca agccatgacg ataaaaaata ttatatcagc cactctttca
     4561 atcataattc tgttttcccc cgacctggca gcacagaaaa aagagcagga cctccggaga
     4621 gagattacct tgtataaccc atataagcct tcactggctg attttaagaa aaaaagctac
     4681 ctgcctgaaa ttgatgatac cgtcagggta atcccgaggt tcagttacaa tgtgctgtcg
     4741 aggccttatt cacccgagtt cagcatcagt acaataaagc ctgcggcact tctttctgat
     4801 ccgctgccaa agctttacaa gggttatgta aagcttgggc tgggcaatta tgcaacacca
     4861 ctggcggaac tcagcatttc aagtcaccgt tcaagacagg gagccatcgg cctttatggc
     4921 aggcatcttt cctctttcgg aaaactcccc ctacggttct ttcctgaaag agtttttgca
     4981 ggttttatgg ataacgaagc atcaatatat ggaaagaagt ttctgggaaa cagcatcctg
     5041 ggactttccg tggattatga ccagaaaacc aggtatgcat acggcttcaa aacagatgaa
     5101 cctgcatatc tctataagcc tgataagaag gaaattaaaa ctggatatta tgatgtggga
     5161 gtaaaagcag tattttcatc agctaatctt gattctgcag atttttccta cgatttcagc
     5221 ctgtcgcacg attacttcaa ttattcaaaa tatcgtacct tcaaccatac tgccttttcc
     5281 ggtgaaatgg ccaaatccat tgaaaagctg tacgcaggcg ccattctgga ttttgattat
     5341 tacaagatgg ccgactcgtt gcatatgaaa cccaggtaca tttttcacgc aaatcctttt
     5401 atccgcagat caacaggaca atggaacttc aatctcgggt taaggttcgt gctggcccgg
     5461 gatattgaaa gctccgccag gccttatttt tatccggata ttggctttgg ctttaccatc
     5521 gtgcccgaat acctgggatt ctttacaaag cttgacggca ggcttgagaa taacgatccc
     5581 ctgaatatgt tttcactcaa tcccttcctg cgtcccgaca ctctcttcag gatgaaaaac
     5641 actgatcatt ccattgcgat atctgcaggg cttaaaggaa acagcggcat ggggggaagc
     5701 tatgtgctgt cagcatcatt ttcacatgtt aatgacctaa tgttgtttgc caatatagcc
     5761 catcccgata ccctgggaca tattgaaagg ggcaattact ttgttccaag gacagatgat
     5821 gccgagatcc tcaatattca cggagaactg agcggaaata tttctgaaag gcttaaattc
     5881 ctgacatcgg gtaacttcta ttcttatacg ctggcattca acagccatcc atggggtaag
     5941 cccgtatgga acggaagttt tgagctgaag tataatcttc gggacaagat actggccgga
     6001 gcgggactat ccctgcttgg caagagatat ttcctggtac ggcaaagttc aaccggctgg
     6061 gccacactgt ctgacgaagt gattgaaacg cctgcccatt ttaacctgag cctgagtgca
     6121 gagtaccggt ataccagaat actgtcattc tgggtgaaag tcaataatat ttccacaaga
     6181 cggtattatg aattggcgtt ttatccctcg cagatgttca gtttgatggc cggtttcagc
     6241 tacagcctct gatgccggaa caaccggcat gcagcatctt cagcggcatt gactggatct
     6301 tccggggatt gacagcagct tccggcatta ttatctgatt catatgcccg ctcccgcgtc
     6361 tcctctctct tccactcaca ctcacactca ctctcacact cacactcact ctcacactca
     6421 ctctcactct cactctcact ctcacactca ctctcacact cactctcact ctcactctca
     6481 cactcactct cacactcact ctcacactca ctctcactct cactctcaca ccttctcatt
     6541 gttgatttct ttgagaaatt gtgcattact tttaacaatc gtttaactaa aggcagttta
     6601 attattgcta tctttgtggc gattattaag aagctttaca tcagtgattt acggtagaaa
     6661 taatttgctg gcgcacgtgt ttacaactgt tgtcgctggc tattgctgta gccgtgaaaa
     6721 accggagtaa ggcaggactg ataaattctg aaagatgaaa taatctgttg actgagatga
     6781 gtgaaatgga aaaaaaattg caggatagtc ctgaatactc tgcggagagc attcaggtgc
     6841 tggaaggtct tgaagccgtg aggaaaaggc ctgcaatgta cattggcgac attggggtaa
     6901 agggattgca tcaccttgtt tacgaagtaa ttgataattc tattgatgag gctcttgccg
     6961 gttactgttc aaggattgat atagtgattc atgaggataa ctcgataacg gttaaggatg
     7021 acggccgggg gatccccacc gggatgcatg aaaaggaaca taaatcagcc cttgaagtag
     7081 ttatgacggt tctgcatgcc ggaggaaaat tcgataaggg atcctataaa gtatcgggag
     7141 gattgcacgg agtcggcgtt tcatgcgtca atgccctttc agagcagctt attgccacca
     7201 taatgaggga agggaagata ttcaggcagg aatacaggca gggtaaaccg gtaactgatg
     7261 ttgaaacgat cggcggcacc tccaaatcag ggacagaaat aaaattcaga cccgatcgtt
     7321 cgattttcca gacaacggat tttaattttg agatcctttc agcccgcctg cgtgagctgg
     7381 ctttcctgaa taaaggcatt gttctgacat taagggatat gcgcgagctg gtggagaacg
     7441 ggaacggggg aaaggagcgt tatatggaat ttcactctga tctcgggctt gtagaattcg
     7501 tcaattatct tgatgccaac cgcgaaagga tcattgagtc accgattcat atcacatttg
     7561 ataaaaccga gatacctgtc gagatagcgc ttcagtacaa cagttctttt tcagagaatg
     7621 tacattctta tgtgaacaat ataaatacaa ttgaaggcgg cacgcatctt gccggtttca
     7681 gaagagggct aacgcggacc ctgaagaaat acgctgaaga ttcaggtgct acagcaaagc
     7741 ttaagtttga tattaacgga gatgatttcc gggaaggact gacagccatc atttccgtaa
     7801 aggttgcaga accccagttc gagggacaga caaagaccaa actcggcaat tccgaggtta
     7861 tgggggcggt tgaccaggca gtgagcacga tgctcacgaa ctacctggaa gagaatccca
     7921 gggatgcaaa gatcattgtg cagaaagtga tccttgcggc tacggctcgt catgcagcaa
     7981 ggaaagcaag ggaactggtc cagcgcaaga atgaattttc aggttccggc ttgcctggca
     8041 aactggctga ttgcgctgac agggatcctt ccaggtgcga gatttatctc gtcgagggcg
     8101 attctgccgg aggaactgcc aaacaggggc gtgacaggag atttcaggcc attcttccac
     8161 ttcgcggaaa aatacttaac gtggagaaag caatgcatca caggatttat gaaagtgagg
     8221 agatcaagaa tatcttcacg gctctgggcg tgaatattgg taccgacgag gacagcaagg
     8281 ctctcaacac ttcagggcta aggtaccaca aaattataat aatgaccgat gccgatgtgg
     8341 acggttcaca tataaccacc ctcatcatga ctttcttttt cagatatatg caggatctta
     8401 tcaggggcgg gaaccttttt attgcaactc cgccgttgta ccttgtaaaa aaaggaaaaa
     8461 ccgaaagata ctgctggagc gaagaagagc gtgatgccgt ggtcagcgaa cttgcacagg
     8521 gaaaagaatc atcggtgagc attcagagat ataaggggct tggtgaaatg aatgcagagc
     8581 agctttggga aacaaccatg aatcccgaga cgagaaccct gcgccaggtt accatcgaaa
     8641 gcgccgcaga agctgatcat attttttcaa tgctaatggg tgacgaggtg cccccgcgca
     8701 gggaattcat tgaaactcat gccagatatg ccaggatcga cgcctagacc cccaaaagct
     8761 ttctcctgat cacaactgca ttatgcttgc agacttcact gcagcaataa cacctgatac
     8821 acgcagcgtc gttgagaaca acatgattct gtttttcagc atgcattctt aatgcattcg
     8881 agggacagat cttaatacat tccccgcacg acgtacagtt cctgtgtata aaaaccggcc
     8941 ttcggtcaag cttccgcatg aaactgatcc tgttcctgag gaacctgatc gaaatatttg
     9001 tattgcgttc aattattatt ctgcggaaat ctttttttat gatggattca atgggcggac
     9061 cggcataaac aatatcatcg ggagatccga gccatacggc cctggcaagg gcaattcggt
     9121 tagtcggtat atcccaggga ttgtagccaa ttatctccga tgcaattata tcaagcgcaa
     9181 gcgggttttc cgagccgatc agaacaccgg tgtttgccgg ggtaccctgg ccgggccccc
     9241 ttccttccat tcccatgatc ccatccatca ggaaaaaatg cggcgttact ctttcgttga
     9301 gatcgaccag aaacctgctg aaactttccc tgtcctgata ccttgcatgc agctttgcct
     9361 tcgaaaagcc cggaacgagg ccaagggtgt tcttgatcgc gccggtaaaa taaaccagtt
     9421 catgattttt tagtttcggc agcgaaatta tcaagtcaac ttcattgatc actgaagcaa
     9481 tcctgatttt tctgtccctg actttcatca aagcggcatc aatcgaaaaa tcaatccatt
     9541 cgatcccgta ttccctgcag atattttcaa ttcccgactt ctcagccctg aaccccctgg
     9601 tatgaatggc cggtgaatcg ccgagaacaa cttttgctcc ttcatcgcgg aggaacctga
     9661 ccatggcttc aacgaccacg ggatgtgtac tgatgcattt ttccggtgga ttgtcggaaa
     9721 gaatattggg ttttaaaaga actgtcctgt tcctcaggcc tggcccgccg cagaatccaa
     9781 atatccggga aatgtgagta taaacctcat taacatcata aaccgtgcat ttcctgacag
     9841 ctactttcgt attcattgat cgttggcaaa agtcgcaaat atagtttatt gtcaggaaga
     9901 atctgctaaa aagtcagttt tgcaatatag tctctttcaa tatttcttct atccccgata
     9961 cgatcctgcg cggcggacct gaaaaattgt ttattatgat actgaaggcg agaacacggc
    10021 ccgacattgt ttcaatatat cccgcgtaac tgcgggttcg cgtcagtgat ccgctcttgg
    10081 cccttaaccg ggagctgaat acaggatctt tgaaataccc cgccagcgtg ccgtctttac
    10141 ctgcccgggg aagcgatgaa atgaactgat tgaaatatga ctccctgttc actgcgtagc
    10201 acaggagaga gaccaatgtc ctggcactta cagcattgaa tggtgacaaa ccgctcccgt
    10261 cctcaatgaa cacacttttt gtatttattc ctgcctcttt cataaacttc aatacaatgc
    10321 ctgttccgga agcagttgat ccgttgttca tgaaaacccg gcccagctct ctcaccaggt
    10381 gttccgcata aaggtttacg ctttcatgat tcagtatccg tattatgctg tcaagccggg
    10441 gagaatttgt ccgggctatg attacaatat ccccgggaac cctattctca attcttgagg
    10501 ttgaagggcc tccctggatt cttattcctg ctgaatccag tctgttcctg ataatactgg
    10561 ccagcaaacg gggaggatca ggtattgaag cccgaagaac aaaatcattt ctgtttacag
    10621 ggatggttcc tgaaatccaa cctgtattac tgtaaggagc ggcataaaca tagccattgt
    10681 cggtgctgcc ttcggcccgg agtttgtttg tcagttcagt tgccgcttcc ggcggattga
    10741 tcctggttat ccggggaatc gtgccggtac ctcctgtccg gaaatggacc tcataggtgt
    10801 tgtcaaaaac cgacaatccg aaggctccgg caccataata atttccaaga tcttcccaca
    10861 gccattttga gggaaccggc tcgttgtcgt accgcgaatc atcaacaatg acttttcctg
    10921 caatttccct gatccctgct tttctgatct caccgatcca gtcatttatg aaatcaccat
    10981 aatgatccct gaagtatgat gatccgagag ccggatcccc tcctccggtt ataatgatat
    11041 ctccctccag tttgccggtt cttttgtcga ggatacctgt gtagcccagg cttgtcgtaa
    11101 aggtgtaggc ggggccgagc agctccagtg cggcaaggga agtaatgagt ttcattgtcg
    11161 aggcaggcat taggctttgc gtgggattat attcaaaaac cgtgtttccg cttcccgcat
    11221 caaggataca cattgacacc gatgcacccg acattgagga atctgacagt atcaggtcaa
    11281 gcgctttttc ctgcccgttc aggttaagaa cgatatgtat acttattatt attatcagct
    11341 tcctgtacat tacctgtcag ttttgttttg cttcaaggtg gaaagaagac cgcatgctgc
    11401 ctggatgtca atgccccggc ttgcccttat agttgtaaaa atgccttttt ttgtcaatgc
    11461 atccctgaat tcaatgagct cctcaggttc tgggctctca agctccgaac ccggaacagg
    11521 atggaagcgg atcagattga tcctgcagcg gatgccatcc agtatccttg caagctccct
    11581 gatatggcgc ggagtgtcat tgatacccct gaaaacaatg tattcaaacg agatccgcct
    11641 ttgattgtca aaatcgaagt tccgtatcag ggtcagtatt tcctttatgg ggtttgtttt
    11701 ctgaactggg accagcatcc ttctttcatc ttcaaaaggg gagtgaaggc tcactgcaag
    11761 gttgaccctg gtgttttcaa gaaattccct gatcctgtca ctgatcccga cggtgcttac
    11821 cgttatcctg tgaggactcc agccaaatcc ccattcaccc gtcaggattt ccaggctttt
    11881 tatgacctca tgcagattat caaggggttc gcccattccc atatatacta tgttggtcag
    11941 tttcctgaat tccggtatgc ttcggaactg attcagtatt tcatttgaat tcagattacc
    12001 ctggaatccc tgcttcccgg tcatgcagaa gatacagccc attttgcatc cggactgcga
    12061 tgagatgcat attgtggccc tttccctgtc ggggatgaat gctgactcaa cataattccc
    12121 gccagatacc ctgaacagat acttccttgt gccgtcacgg ctttcggcaa cgctgaccgg
    12181 ttctgaaaga ccgaactcat attccgccga aagaagattt ctcattcttt ttgaaatatt
    12241 ggtcatttct tcaatagtcc gtatctcttt tccatacagc cattcggcca tttgcctggc
    12301 agaatatccg ggcatgccaa gcttttttgt tacggaaatc agctcattga gagtcttgcc
    12361 aaacagattt tcctttgtca tcgcagatca gatttgacgc catgcagctt tgcaggaaag
    12421 ctgctgttta acaggagcag aaacacagaa tttaattcag tacattgttc aagtacccag
    12481 gcttgcttta ataaacaagg tcatatctca actctgcctt caaatacaaa ttttgcaggg
    12541 cccgagagcc agacgtcagt gatcatctct ttacggacga tgaattttac gctaaggctg
    12601 ccgcctctgg tcctgacatt tacaggcgaa ctgacaattt gtccagtcaa tactgctgca
    12661 atggcagaag cagttgcgcc tgttccgcat gaaagtgttt catattcgac tcctctttca
    12721 taggttctga tgtatattcc tcccggcaca ggctgaatga aatttacatt tgttccgtcc
    12781 gggtgaaaat cagcggaata cctgatcttt ctcccttcag tgttaacatc caggctgtcg
    12841 atatcgttgt ggaagaccag gtagtgcggt gacccggtat tgatgaaata attatttccg
    12901 atccttgaaa tattcccgac atcggtcatt ttgaggtcta ctgatcctgc atcggcgact
    12961 gcccggtgta ttccgtccga agccctgaat actatacttg taccggcaat attgttcctt
    13021 tttgcaaagc cggcggcaca tctgcccccg tttccgcaca ttgttccctc atacccgtcg
    13081 gggttgaaat acttcatctc aaaatcatat tcagctgttg cactgttgat gagaatcagt
    13141 ccgtcggctc ctattccgaa tctcctgtcg cagagttcct ttatcagttt atggttgccg
    13201 ggattgaaaa tatcccttcg attgtctatt ataatgaaat catttcctgt cccctggaac
    13261 ttgttaaagg tgatcatatt cattgttaaa gtggtgttaa atatttgcag ataaatcagg
    13321 aacaagaaaa aaatgcatga actttgttaa acaaatgttt ttaaaatcgg ttataaagat
    13381 atttattctt ttaagaagat aaattatctg aaaaatccgg aaaaacagaa gtacaagaaa
    13441 taatattatg attatgaaag caaagaattt gattggtgcg tttgcaatgg ctgttcttgg
    13501 tgcggccatt gctctgtttg cgtactccag aataaatagc aattccgtgg gagcagttgc
    13561 cagggacagc tcgggtattg agctgcgggg cgctgatacc tatcttacat ccctttcgat
    13621 gcaggagcgt cccgtggatt ttacctatgc cgctgagcag acggtccatg cggttgttca
    13681 tgtgactaca aaatcgatgg ttggccccca ggccagcaat cccattatgg aatggtttta
    13741 cggggaccgc tattccaagc caagggaggt tgcaggatat ggttcagggg tgattatttc
    13801 agatgacggc tatattatta caaacaatca tgttattgat aatgctgata acgttgaagt
    13861 aaaattaaat gacaacaggg ttttcacagc tgaaatcatt ggccgcgatc ctacaactga
    13921 catagcattg ttaaaaataa aggctaataa ccttcctttc attaaatacg gagactcgga
    13981 ccagttgaaa atcggggaat gggtactggc agtcggcaat cccttcaacc tgacatcttc
    14041 ggtcaccgcc gggatagtaa gcgcaaaggg aaggagtctg ggtattcttg atgccgatct
    14101 caggatcgaa tcattcatac agacagatgc cgcccttaac ccgggtaaca gcggaggcgc
    14161 cctggtcgat acccgggggc tgcttgtcgg catcacatcg gcaattgttt ctccaagcgg
    14221 cgcatatgcg ggcaattcgt tcgcaatacc ggttacaata gtcaggaaag ttgttgaaga
    14281 tcttaaacag tttggcgaag tacagagggc cattatagga gttaatatca ccaatgtgtc
    14341 gcaggatgat gcaaagaaac tgaagcttga cgaggtcagg ggtgcaaaaa tcagcaatgt
    14401 tgttcccaat ggtgcagccg aggccgcagg actgaaggaa aatgatatta ttataaaatt
    14461 caacggcgtt tcagtggcat ctccgtcaga gcttcaggag caggttggaa aattccgccc
    14521 gggcgacagg gccagcatta cctacattag gaacgggaaa gagaacacag ttccgattgt
    14581 cctgaaaaac attgccggaa atacaagcgt tgtaacccgt gaaatgagcg gcagcgggac
    14641 tgtcttcggt gcacggcttg agtcgctggg gtcatccgac agggaaaagt acaacatcga
    14701 ctacggtgtg aaagttgctg aactcaagga cgggaaattg aaggacctgg gagtaaaaaa
    14761 aggatatatc atcctgagca taaacggaag gaaggtcaag aacgcgtccg aagttcgcag
    14821 tgcaagtgat aatgagtcca atctgaaatc gatagagggc attcagtctg acgggacatt
    14881 cttcagctat tccttcagga actagggatt ccccggattg aagtgattga tggtcagtat
    14941 accggcaaat aaaagtattc tggccatcaa cattctttat tttaatttgt ttatatattg
    15001 taaaagtagt aacttcgcgg aaattttttt ggagacataa tgagacagct aaaaattacc
    15061 aaatccataa caaacagaga aagtgcatca ctggacaaat atctgcagga aattggtaaa
    15121 gaggagttga tatcggtgga agaagaagtt gaactggcgc agagaataaa gaaaggagac
    15181 aggtcagccc ttgaaaaact taccaaagct aatctcaggt tcgttgtatc ggtcgccaaa
    15241 caatatcaga atcagggttt aagtcttccg gatcttatta atgagggaaa tctcgggctt
    15301 atcaaggcag ccgagaagtt tgatgaaacg agaggtttta aattcatttc atatgccgta
    15361 tggtggatca ggcagtcaat ccttcaggct ttggcagaac agtcgcggat agtcaggctt
    15421 cctcttaacc aggttggttc acttaataaa ataaacaagg ccttttcgcg gtttgaacag
    15481 gagtacgaac gtacgccttc acccgaagag ctcgctgaag ttcttgagct gccgaaggaa
    15541 aaagtaaccg acacccttaa agtgtcaggc aggcatgtgt cggttgatgc accatttatc
    15601 gaaggtgagg acaacagcct cctcgatgta ctcaccaaca ctgattcccc ggttgccgac
    15661 aaactcctga tggatgaatc cctcagcagg gagatttaca gggcccttgc taccctcacc
    15721 gagcgggaaa gggatataat caggtttttc ttcggaattg gctgccagga gatgactctc
    15781 gaagagatag gagagaaatt cggccttaca agagaaagag tcaggcagat caaggaaaaa
    15841 gccatacgca gattaaggca tacatcaagg agccgcctgc ttaaaggata tctcggctag
    15901 ataaattaac gcaataaggc attacaggga ccctgatttc agaggtccct tttttgatcc
    15961 gtgcatcgga ttccatgctt actggtatga actatgctgc ccggcggaac ttttccggaa
    16021 aaccacctgt tcacgagcaa ttcgttcatg aaaggattga ttgatcagat cccgcttctg
    16081 aaagccggct gcggcctgcc agatatgccc tgtacacata atgcagctta atttcgcaat
    16141 aattgccgct catgacaaga tctgccgtta ccctcctgat gagataaacc ggccatttca
    16201 ggctgactga aaggaataaa atcaaaagta ctcgcaccca ggtcaatttt tcaaaaggtt
    16261 tcatcaaaat tattatgata gattgctaat ttcttttaac ttatactctt tatttttcgg
    16321 taaatcattc gggaattgtc ctgaaggcgg atattaagaa cagagtttac ggttaatgat
    16381 aaattgtttt ttcaggatat tattaattcc tctgttgagt ttcactgtgc cggatcgtgg
    16441 taaaatgtct gaagaagcaa ctttacctgt cttacagctt ggacccattt atttcttcta
    16501 tagtgttccg gcaagaatta aactgaatga caggattgac ctcagctttt ccagaagctc
    16561 attgataacc acctacaggt acagacttga gatcccaaca gaagggatga cagggatcga
    16621 tgataaatta ttatcatggt cgttatggac aaatgaagat tttacagcga tcattattcc
    16681 tgatctcgat gttcttggta cctacaaatt cactgtcgaa tacaaaacag gtacaggcac
    16741 actgacaaga cggtttgaga aaacctttga agtttattcc ctttccaatc tttcatctag
    16801 ggccaggccg gtaaaacctt cacaggaatt tccggttaaa aaagaggcat taacgcctga
    16861 agtaactcag gcagaacctg gttacaatta tccaaaaacg gctcaggcat ctgctgcagc
    16921 aacaaaggag gaatctttga caccggtgcc aaataaaaga atatcagttg cagaagcccc
    16981 ggccacggat aatacttcag tgcgcaaaga tacacaatca accaaacaac ccgatatttc
    17041 tgcggccccg gttcaacgaa cccccataaa caaaaagatc cagccgtcaa atgtttcaaa
    17101 caggaagaca ccacagattg aaaaactggc cttcccaacc tctgatattt ccttatttag
    17161 attatatact gcaacaggtt ttataaaacc ggttaattac agttttgtcg attctgctgc
    17221 aaatggtgtt gttactgagt cggatccggc tattgttgca agagtgcccg ttatcattga
    17281 aaaaataaag gttgacaaac caaccggcgc ttacctggtt cctgaaaaga cgaaagctcc
    17341 tcccaccctg gttgcctacc atccgccgca ggtaataata agtgaccaga caataaccgg
    17401 agaagcgaca ggtgttgagg tgtatacttc aatcacaaat atggcagacg atgccggaat
    17461 acagtcagaa ataaaaataa gtgatacctt gataagtcac aatgaaccgg gccccggaaa
    17521 aagtgaaatc cagatggaag accttgcggc atcggtcgga accattgcag gaaaacctga
    17581 atcagacatt gctgaggatt atttgctgaa attattaccc ccaacttccg gaaaaactgc
    17641 tgccagtagc tttaatgaca tgctgcttaa ggccattgag gaaaatgata cggcattatt
    17701 cagggaatca atactgaatg gaggaggaaa tgacctggca gggattaatg gcggtaatgt
    17761 cttccatttg ctgaatggaa ccatcgtgga tgagtattcg gttttcctgt taaaaaagaa
    17821 cggtaattca attgacaaaa aggacaggaa cgggaacagt cccctgcata tggcgattct
    17881 gaacagggac tttgaatacg cgaaatgcct catcaatcag ggtgcagacc tgaaccttat
    17941 gaataaaatg gaactggctc ccctgcacct ggcagctttt cttaacgaat accggctatc
    18001 cgcattcctg atggcaaagg gagctgttgc tgacatagca ggcaattcag gatatacccc
    18061 gttacacatt gcttcagagc tgaataacca gacaattgcg gagcagttat tggaccactg
    18121 agcaagcgtc aggcaaaaaa acgaccaggg actcactccg gttgccattg caaaaattca
    18181 gaaaaacaag gagatactca ggctgcttac cggctcaggt attaaggaaa cgaaaaataa
    18241 tccgattggg ttcacaggga atacgaatta tcatgtaatg gatcccatgc tcagattcag
    18301 ccttccatac aacaacgcgc ttgtggccaa aagacagcgt tacacaataa cacaggctgt
    18361 tactgtaccg attgcagcaa ttctgtctgc agggacggca tatctgaaat cagaagcaaa
    18421 taaatattat tcattgtcaa aaattgctga gacagaagaa caggcaagag cattatatga
    18481 caaaacacgg aaacttgaca cctcagtcta tgttacagga agtttgacac ttgcctcagt
    18541 gtatggattc attcactcca cgatcggaaa aagaaaagtt accgggaaaa tgtataaaac
    18601 cttcaactag ggcatatgat tcacaaaaga ttaatacagt ctgctgcatt gttcattctg
    18661 attattctca ttgccggctg caggagagag gaattcagct ggggaattaa gattggggac
    18721 attacatact caggtaacgt tgttttcctg ggggaacgtg aactttcttc attgaatgaa
    18781 attacttccg atcagatggt ctttacagat aaaacaggag aaattgaaaa aatcactgat
    18841 aaaagcattc tggtcatcgg catttcagaa aatacgccct atggttcgct cagaaaagtg
    18901 aacagtatcc attctgaggg aaatcttctg acggtcagaa ccgttcccgc actcctccct
    18961 gacgcaataa aggaaggaaa gatcaactta aagagaaagc tgcttgaaaa ggatttcaga
    19021 ttaaaattta aagcggatgg agttaaaatc aaggatacga gcaaatcgtt tgaaggactg
    19081 gcagttacat tggataagtt aagtatttgc gatgatgggg catgcaatgc cgtcattgac
    19141 ggggcagtgg gaataacaac gaatatcgac atcggaatgg attttaaatc caacaggctt
    19201 accgggattg tttttgccac gacccttgaa aagatcgatg aggtgacact tacctctacc
    19261 cgggctttta gcggggcatc gggcaaagtt gcagccgagt ttattcattc tccgataata
    19321 atcgacagcc ttgtctttgt tgcagaggta aaaatacact gtggtttcaa gggtacagca
    19381 tcatctgcaa tcacttcagg cgtacgccag gacagaacta ttagcacggc tgcccgctat
    19441 ctaagtgcct catggtccga cgatcgtctg gcccactctg aaaactttga ttatatcaca
    19501 ccgctggtga cagatgctgc agatcttaag gtaattacgg gaccggagat cacagtcttt
    19561 ttgttcggga tacctgtcca gactattaag gcagatgcat attattcagt gaatgcacaa
    19621 aaggacaata ccccttcatg gaaactattc agcggcttgg atggtcacgt tactgtaaat
    19681 aacgagatac ttggcatggg gcaagatcat attctcagca tggtgataca ggcatcagaa
    19741 atagccagtg ccggtggcaa gtagaatggt attcttactg ctttagtgtt ttatcagggt
    19801 cagggtttta tgatcctccc catcactgat ggttacaaaa tactgaccat ctggaaatcc
    19861 cgagatattc agttctattt tgtttcccgg atcaaacctg aaataatttt taatgaatac
    19921 agtattaccc agcttgttgg tgattgcaat tactatcctg tcaaatttct cccctggcag
    19981 atcaacattg agcaatgtgc ttgccggatt gggatatatg gatacagctt ccagttttga
    20041 ttcttttaac cgggaaaccc ttcctttctg actaataaaa agcaccattt caggattaag
    20101 ggaataatgt atctttaatg caccgatctt ttcaaacacc gagatattgt cgagatatgt
    20161 gacttttatc acagaagtac cgggttcttt cacagctttc atccacattg aattttcact
    20221 taccgtccat tctgcatttg attcaattct gattgaaagc gaaccggctt cggaatttcc
    20281 gattctgatt gtatcattgt gacgaagtat tttatcgccc ggaccggttg ccttgagtgt
    20341 atagatgcca acagccggag atggggaaga tgtgcatcca tatgagttgg tgaccgtaaa
    20401 agtgtaaata cccgcgggaa gacccgatat tgctgacctt gttcctgttc cggttattgt
    20461 atttccgcca ggacttcgtg tcagcaccca tgttcctgat gcaggcaatc cgccaagaac
    20521 tgcactgccg gtcggacggg attcaacagg ctgtgtgata ctgacgactg tcggagcaga
    20581 gggggttggc ggctgggaat ttattaccgc atttgaactt gagcttgaaa tacatccgta
    20641 tgaattcgtt actgtaaaag tgtatctgcc cgacgaaagt cctgcaacac tgaagctgtt
    20701 tcctgttccg ctgtaggtag taccaccggg actaaccgtc agggtccatg ttccggactc
    20761 gggtaatccg tcaagagcga cagttccggt agcgttattg caagtcggat gattaatgtt
    20821 tccaattacg ggagcggcag gagtaggggg ttgcggatta attgttacat tacccgaggg
    20881 tgcggatgta cagcctgatt catttgttac ggtaaacctg taagtattgg aaggaaggtc
    20941 gtggattgtt gtggttgcac ctgtacctga atatctgatt tccccgggtg cccttgttat
    21001 ggtccataca cctgatgcag gaaggccgtt caagacaatg cttcccatgg catttgtaca
    21061 ggaagggtgc gttacggagc cgatattcgg aggcacgggt gttaacggct ggggattgat
    21121 tatcacattt gccgataccg gcgaggtaca gcctgattca tttgttacgg taaaagtgta
    21181 agtattgggt aaaagtccgg ttatagtggt tcttgacccg gtgcccgttg tacttatatt
    21241 gccgggatta cggaccagcg tccatgttcc tgctgaaggc aatccattca gggtcacgct
    21301 gcctgtttct gttgaacatc cgggctggac aatcgtttct gccgctggag cggccggggt
    21361 taccgggctg gcatttataa taacactggc tgatggagct gatgtgcagc ctgctgcatt
    21421 tgaaactaca taagtataat ttcccggagg aataccatga agagtgatac tggttcctga
    21481 agaggatgtt gacacacctc cgggatttct cgtcagggtc catataccgg cagatggcaa
    21541 accactgaca accaggctgc ctgttgaagt gttgcaatcg ggttgagtaa ttgcgcctgt
    21601 cacgggcggt aatggagttt ctggttgtgt ggttattata acattcgccg acggatttga
    21661 aacacagccg gctgcatttg ttacggaaaa tgtgtaagta ttggctgcaa gccctgagac
    21721 agttgtcgtt gtgccggttc cggacattgt agccccgtcg ggggtccttg acaatatcca
    21781 tgtaccttct gaaggaaggt tgtttaatac aacactgccg gttgccctgg cacagtcagg
    21841 ctgggttact gaaccgacag aaggtgactg gggagtcgca ggccggacat ttatgaccac
    21901 attgccggat ggggccgaag tgcagcccga tgaatttgtg accgtaaagg tataggtatt
    21961 ggctgcaagg cctgatattg ttattgatgt tcctgtgccg gtataggtgt taccgcccgg
    22021 ggtccttgtt aatacccacg tacctgttga tggcaagccg gtaattacaa cactgcctgt
    22081 tgacacggca catgatggct gggtgattgc tcctacggaa ggagctgccg gaacaggagg
    22141 aggtgaatta atgaca
//
LOCUS       DAOOYG010000035        21752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2253_length_21752_cov_7.625063, whole genome shotgun sequence.
ACCESSION   DAOOYG010000035 DAOOYG010000000
VERSION     DAOOYG010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21752
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2253_length_21752_cov_7.625063"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1885)
                     /locus_tag="PK288_04860"
     CDS             complement(<1..1885)
                     /locus_tag="PK288_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPM18283.1"
                     /translation="MIRQGGWDIVVLQDHSRYPSEPLSFVEEYVYPYAHFLKEEAQSY
                     STNAEIMFYMTWGRRDGDADRCPENPQVCTYIGMDNLIRERYMIMAESNNASISPVSA
                     VWRYIRKNYPSIELYHPDGSHPSDAGFYAAACCFYTAIFKKDPMEITFNYNLNSTDAN
                     RIKTAAKLVVLDSLSKWHLDKEYPDIEKPTVPTNLSSGNITSDGFTLSWSASTDNVAV
                     TGYDVYRNGTYLKTVSSTSSDISGLSAGTTYSMTVRAKDAAGNISDPSAALSVRTKDA
                     PDLQAPSIPTGLAASNISPTSFSLTWTASTDNVGVTGYDVFCNGTLTSSVSSTSSTIT
                     GLSAASTYSMTVKAKDAAGNISEPSAPLNVTTLSAPDTEAPSTPTNLHATNITATGFT
                     LGWNESTDNIGVTGYDVYLNGNLARSVTATYANITGLTSGSTYTMTVRAKDAAGNLSA
                     LSQPFEITTPDNQPPSIPSGLTATNVTQTGFILNWSASTDNVGVTGYDVYLNGTLYTS
                     VTGVSATISGLTASTSYAMTVRAKDAAGNISSASNVFNVTTANPPDTQAPSVPTGLNS
                     VNITETSFTLTWSPSTDNIGVTAYDVYRNSILFSSVTTNSALISGLTVATTYTMTVRA
                     RDAA"
     gene            complement(2785..3888)
                     /locus_tag="PK288_04865"
     CDS             complement(2785..3888)
                     /locus_tag="PK288_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM18284.1"
                     /translation="MKTYISKLASLIILVSACSSPGPESPGRIREADGILLKNYRPVS
                     LYKIPVTHIEKAAYPVFDAHSHNYATNSEELEEWVKIMDECGIAKTVVHTQASGKLFD
                     SIYNLYSAHSDRFQLWCGFDYTGYDQPDYGSAAVRELERCYKLGARGVGEEGDKGMGL
                     TFNEPVQARGMHFDDPRLKPLFEKCAELKMPVSIHVADPIWMYEKMDSTNDGLMNAQT
                     WRIDMTRPGIIGFDALVQTLENAVRDNPKTIFIACHFANLNHDPGKLGYLLDKYPNLY
                     ADISARFAESATIPRTMAAFYEKYQDRLLYGTDMGYDQEMYRTTFRILESADEHFYDI
                     NLFGYHWALNGFGLSKDILKKIYYDNAARLFGN"
     gene            complement(3885..4712)
                     /locus_tag="PK288_04870"
     CDS             complement(3885..4712)
                     /locus_tag="PK288_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPM18285.1"
                     /translation="MKIKDKLYQLKSLKKAVLAANFYNFETLSGILLASVRLKLPVIL
                     QLSESSIQYMGLDVASSMAKAAIREYGAEAWLHLDHGSTELMVRKCLDAGFDSVMIDA
                     SEKPIAENIEITGHVVEIASRYGACVEAELGYVAKLGQKQDNEGFTQPDDALYFVENT
                     GVHSLAVSIGSAHGFYRQEPRLDIGLLAAISKRVPVPLVLHGSSGIPDPMLQEAILNG
                     ICKVNLATEIKNTYISTLRKLLEENDEIDLRKIFPPATHAVTELITDKLETINNIRL"
     gene            complement(4713..5672)
                     /locus_tag="PK288_04875"
     CDS             complement(4713..5672)
                     /locus_tag="PK288_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPM18286.1"
                     /translation="MIASEKKFDVCVIGELNVDIILNDIGNLPQVGKELMAGYMEVTL
                     GSSSAIFASNLSALGTSVAFIGKIGEDNFARVVIDSLSARNVDTSRIISSAKLNTGAT
                     IVLVYDQDRANITYPGAMYDFKIADIDFNFLSRAKHMHFATCFMQPGIKKDLPSLFSR
                     ARELGLTTSLDTQWDPDEKWDLPLDQLLPNVDIFLPNMQEFKYLTHSNSLGEGIEKIR
                     DHANLIVVKNGNDGAYGWDGTHIVHQPAFINNNVIDTVGAGDSFDAGFIRDFIGGKPF
                     LKCLETGALAGALNTTMAGGTGAFRDPETINKLSIDRFGRPLV"
     gene            complement(5711..6976)
                     /locus_tag="PK288_04880"
     CDS             complement(5711..6976)
                     /locus_tag="PK288_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HPM18287.1"
                     /translation="MNKKLRERAAAEGIPLMDHILRRIKALQSETGIKRTIFAACPNS
                     LSVIRAALKSAKRCNAPIKFAATLNQVDLDGGYTGLTPSEFVETVRHHARNLNVTSPV
                     IIAIDHGGPWLKDIHRTGKWSYDETMDAVKTSFEAAIVAGYDLIHVDPTIDITLQENS
                     PIRIETVAERTVELIKHAESFRKSKGLPPISYEVGTEEVHGGLADISTFRKFLRLLKM
                     GLEQNNLKDVWPCFVVGKVGTDLHTTTFDPEVARRLATIAGEYGSLIKGHYSDNVTNP
                     EAYPESGMGAANVGPEFTELEYEGLMELESIQQALMEEGKLKTQIPIKKTLWDAVIKS
                     GRWKKWVQENENPDDFYSISFQRQEWLIKTGCRYIWEEAAVVSARQQLYENLAGSGTE
                     AATIVESAIESAMDKYFWKFNLVGLNSLL"
     gene            complement(6986..8143)
                     /locus_tag="PK288_04885"
     CDS             complement(6986..8143)
                     /locus_tag="PK288_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPM18288.1"
                     /translation="MFPSGSETSLLEELGAVHTAGEIAGQPDLWLEIYSQISADKDRI
                     MSFFTGSLPCTDRIILTGAGTSAFIGLSLSPVFSRHLMIRTDSVATTDLLSYPENYFS
                     NDESIILVSFARSGNSPESTAVTELADKLCKRCSHLIITCDGNGRLANYHTQSKKLVI
                     LLPPSANDRGLAMTGSYSGMLLCGILIARLREMDSLGSQVNKLYSYGNKIIDNYSGKI
                     KEIASADFKRAVFLGSGPFFGTATESHLKLQELTDGIIICKNETYLGFRHGPKAVTDE
                     TTLVVFIFSNNDYVRQYERDLVLSMKKGRKPLRTIGIYESSADGLKFDLDIKLSDSGN
                     QLDEEFLTVCNVIPAQLLGYYKSLALGLRPDNPSKSGAISRIVEDVTIYKF"
     gene            8458..11571
                     /locus_tag="PK288_04890"
     CDS             8458..11571
                     /locus_tag="PK288_04890"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HPM18289.1"
                     /translation="MIKKNFMFLFLVLSQALPGQKASITIETRDILTYPFSDPNPVPI
                     LVERRDEIYPYHSFNGYSLKGRMQKWNVIKLENDYIEVWVMPTDGGKVWGAIEKSTGK
                     EFIYRNEVMKYRNISMRGPWTSGGIEFNFGYIGHNPSTCVPVDYTTVENPDGSVSCIV
                     GSLDLPSRTKWSVEIRVPKDKAYFETRTFWNNPTPLPQSYYNWMTGAAVVSDDLEFFY
                     PGNQEIGHGGEHGLWPVDREGHNIALYRNNNFGSSKSYHVVGEFNNFMGGYYHNSRFG
                     FGHWALYEEMPGSKLWLWSLARDGGIWEDLLTDTDGQYMEFQAGRTHNQYGGTSNKTP
                     ISQVPFAPGLTDRWTEIWFPVKEIGGISDVSPSGVLFAKNENGTLQFGVNSFAFAEGT
                     IIIESGGNVIYREDVNFKPMDVFRSSIPLAAGTEYEISVRGMGLKYNPAKKKFLSRPF
                     ISTMPTDQVTASSVYQEGMENKENRNYKVAKVLLKKCLIMDPLYTDAMAALSELYLRS
                     NLYDSALYYANNALQMDTYHPAGNYFAGLTYKAKGNLIDAVESLGWAARSTQYRSAAY
                     AQMAAIYLQLGDMQLADHYAGLSLDFERNNFNALQVLAIRSRKINAREKAEEYLNTIS
                     SLDPLNHFADFERYLLDPSAENEKLFRKTITNEMPYQTFLELCMVYYGMGFREDAIQV
                     LDKAPSHPLITVWKAYLKDDAAILQEAAAASPAFVFPYRTETVSALEWAVSKNNSWKF
                     KYYLALNYAAIQRDDDAMKLLRECGSEPDYAPFYLTRTSLLRPMTNEQMLTDLQAARR
                     LTPDDWRTAVRLIDYYERTGDNKKALEIATETFKKDRENSIVGIRYAQALINNGLYAA
                     SLRALESMTVLPNEGASIGKVVYEQSSLFLAMDMMGRKKYGEALKYIEKSKLYPENLG
                     VGKPYEPDTRIQDYLKIKCLEKLNRKNEIPELTRSVVDFTKKGIDHPSLSSLLAIRLL
                     ESQGDRAAANDLVQQIRNSDRAGNPVNRYVLAAAGNDQSVLADLEKRMAGNSVFQILK
                     KVEEVTK"
     gene            complement(11732..13573)
                     /locus_tag="PK288_04895"
     CDS             complement(11732..13573)
                     /locus_tag="PK288_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPM18290.1"
                     /translation="MRITSHIKSAVLYLPVQILLILLVSSCSPDPGIKRIWAVDDGEK
                     IKKEDISNPISSEPANATWKNNTISLFAARNEIIAFQLIIQAGKAGAKQVNVSISDLK
                     NGSSVIPGSDASPSDPFDYRGKYVELFTEHYLNMIKRSPPLWFFSETAVPSSYYTGWV
                     PDCLIPFSAPPEKGGAPFSIEPEMNQAVWADILVPENAVAGTYSGKVTVTVSGKKYKT
                     IPLFIKVYDFTLSDTTHLRNMFGFYPGFIAERHNVQRNSEEYFSIELKYNQLAHRHRF
                     DLVCPVNNLNQMDNHYKRYLTGDAYTEENGYWGPGRNIGNGTFSIGYGGGFPAEYGGS
                     VRKMNKKDWWTGSDAWESWFLKNALYVERHKYLFPDEPDFKGPAGAKGTGSMDTIRMQ
                     TGWTHTNPGPGKNIPALVTNKIIPGLKGHVDFWSISAQEWTLNLTAGMVAEERAAGRK
                     LGIYNGFRPGMGAVVSDADAVEFRVMPWIAWKYNIDQYFYWSTTFWTNLNVFVNPLTY
                     EDRINGDGTFLYPGRDALFPEEDRSLDGPLSSIRAKNWRRGAQDFEYLWLAREMGLEK
                     DIEVIVNKCIPAGVWDAANLDDASWPHRGYKFEEYRKELAELIESHK"
     gene            complement(13641..15011)
                     /locus_tag="PK288_04900"
     CDS             complement(13641..15011)
                     /locus_tag="PK288_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HPM18291.1"
                     /translation="MKKIHITGLSAILSVILALGILNACSSNEKIENFVLEEDTGFLQ
                     IPGVGWQTFFRTADKDPSMNGLPFRSGCAYFRWTWGDLEPAEGQYAFGMIDEVLRKCR
                     EANQALAFRVMLSWPGHEGTIPQWLLNKGIRYTYSECPEEGAHYEVDLEEQVVWDYHE
                     KLIRALGEKYDGHPDLALVDIGSVGLWGEWHNYCDPKLMPGDATRKRIIDLYYETFPN
                     TPLTALVDDKDNVLYASLKGRSAWRGDCWGNGDGPGVGWNHHRDSYWPMVKMLPDGWK
                     TGTVALEPCGTFGSFPTPPKSVVDDAIAWHATFIQNKSHFIPKNWLPEIERLMKVIGF
                     RLVLRDIDYNKAPVQGAGIPVSMKWENLGIAPPYRDHRIALRLRNTDGKIQAAVVTDV
                     SIRGWLPGEKKAEALYKLPGDIKPGTYTLELGIVFHSSPDHVIPIANKGKTTDGWYRV
                     GNLEVK"
     gene            complement(15074..16891)
                     /locus_tag="PK288_04905"
     CDS             complement(15074..16891)
                     /locus_tag="PK288_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPM18292.1"
                     /translation="MKRKNKTSLVGSAILCAMLLASCSGARQETEVRLVSISSMERIG
                     QDQPLSGSDQALISGARNEVESFQVVVGAITKNIRVVAAEISDLRGKTASVGRENITL
                     FRAEYARVRRSSPRAELPPGLYPDPLVPFIDPETGKPIEPFSQYRVRWGTPFISSGFE
                     MYALPFDVWKGQNQPIWIDVYIPRETPAGEYTGEFTVTFDNYPMPHGQSAENLKTKKL
                     SLPVKLTVWDFTLPDGPTHRNHFGRVNWQIPRLFGAAGNSERNRDIELRYCRMLADHR
                     INPPIPSSLMPEVNPDGSLRIDPLRHEALSKFIRDLHVTDFEIPGPGFSDMTTANRAK
                     AIRYYRDYYNYVKNNGWDKRAYVYMLDEPNLKENYEEVLALGQMIHEASPDLRCLVVE
                     QTYKQDPEWPDIDPAVDIWCPLWAFIDRESINEKIAHGDEVWSYTALSQRTPPYHPEY
                     EKLKDFDSPYWHIDALLTSYRTPTWINWQYNITGLLYWSAVQISESRTGIMDPWLLPA
                     FSESENQFNGGGYLLYPGVPCGIDGPISCIRLKNIRDSMEDWEYFALLEQLTDRQTVT
                     SLVSEIAPDWWSSSSDPEKIQMIRGKIAGEIARLNNGKK"
     gene            17365..20544
                     /locus_tag="PK288_04910"
     CDS             17365..20544
                     /locus_tag="PK288_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM18293.1"
                     /translation="MSRLAGKESPLNRRIIVVSLLAVLLVSLPQLSRAGAGKNPGSKN
                     DNSAALIMQQRVTGTITDASTGEPLVGVNIVVEGTTTGTMTDINGKFTIEAPSRNSVL
                     SISYIGYVSLKVNVEGRAIIDVSMESDLRALEEVVVTGYGTVRKSDLTGSVSSIKTEQ
                     ILQLPTQRVDQAIQGRAPGVFILNTDGSPGGNTMIRIRGSNSINGGNDPLIIVDGLQG
                     ANINQLNPNDIASMEILKDASATAIYGSRGANGVILITTKLGKTGKPVIDGGYSVGIQ
                     RLARKLPVMNAYDFARYNNFQNSFNTAYGQTPKLYFTEADIEYYKTHSTDWQDEIYKT
                     GIMQTSNLAISGATDRLKYMVSTGYLDHKGILLNSAYNRLSLRANIAADITDWVDFGM
                     NYSYTYEKYKSPPFEANLFTSVNDAPRWAPTEPVYDENGNYWKHRAGYAAYDTWNPVA
                     TALETKIDNPTYQNNLNLFLNFKLLKGLTLKIMGGGTFTSSYNLRYENTKTRNGFANN
                     GVGNITDNFRNAYQNTNILTFDRTFGIHHITITGVTEEIFSRSMGSSMRGINFLVDQL
                     GTDNMDGARAVTVDSWASKRVLISYMGRINYGLLDKYLLTLSYRADGSSVFGANNKWG
                     YFPSGSVAWRISQEEFLKDSETISDLKLRVSYGVTGNQAISPFGTLARLGSGYRYPYN
                     NGDNLNIGFGLSGIANPNLKWESTGQTNIGIDLSLFGGRLTSTIDVYRKVTKNLLMPR
                     QLPDYVGVSSVIDNIGSIENKGIEILIGGDPVAGRFRWNTSVNFTMNRNKVLDLGPGV
                     TRIGYTPTSGGYSLGTEYMFLEVGQPFGQMRGWKFLGLWRSDEEAEARSYGQLPGTPK
                     YADISGPDGVPDGQVDGFDRTTIGYGYPDFTVGWTNIFTYKNLELSFLINASYGNQLF
                     NTIRIRRESSWEGTDPKVWNYWTPENQDTDVPALYDGQWVLDQHLVNKYIFGGGSGAT
                     SRWVEDASFIRLKTLTLAYNFDSNLLRKIRFSKARLYFTGTNLLTLTKYTGYDPEVAQ
                     YAYNDALIGVDQSVYPPARMFTFGIDFTF"
     gene            20565..>21752
                     /locus_tag="PK288_04915"
     CDS             20565..>21752
                     /locus_tag="PK288_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM18294.1"
                     /translation="MKRKFSSTLTIILLSALILFFASSCDQLVEKPTDFVGPDNFFTT
                     PAQIESAFTASMDVLFGGWTFYDWYGYPEIFEMDDQVYGGDLVIGDDFGSGCWQKHFR
                     AISFINPAIKALNAGQVTGISQEKADELMAQAKFLRGWNYFMLVRYYGGVPLIEETTD
                     ATRDDIPRASVAEVYDLIVNDLLFATQHLPVKWGEENKARPAADAAKTLLAKVYITMA
                     GFPLNETSNYAKARDMAKQVIDAGNYWLVPDVEDVFEMENTYGPEFMWCFHNSEEEYV
                     IEPQIWLPGEMAGGWGDTRCEKRWGEAFPEEPRKHAYLLLEDPWFGTGTTYLEWGGST
                     PYVRKYLYDSPENMEKNVSYQSMPILRYADALLLFAEAENQVNGPTQAACVGPFTWFS
                     ASAN"
BASE COUNT         5632 a         4959 c         5203 g         5958 t
ORIGIN      
        1 ccgctgcatc cctggctctt acggtcatgg tataggtagt ggctactgtg aggccggaga
       61 taagggctga gtttgtagtc acgctagaga acaaaatact gttcctgtaa acatcataag
      121 ctgtaactcc aatgttatcc gtggatggag accaggtaag ggtgaaactg gtttccgtaa
      181 tgttgacgga attaagccca gtgggaacag aaggtgcttg tgtatccggg ggattggcag
      241 tggttacatt aaaaacattg ctggctgaag aaatgttccc tgcagcatct tttgccctta
      301 cagtcatcgc ataagaagta gaggctgtaa gtccagaaat tgttgcagaa acgccggtta
      361 ccgatgtata gagcgtacca ttgagataaa catcataacc ggttacacca acattgtctg
      421 tcgacgcgga ccagttaagg atgaaacctg tttgggttac attggttgca gtcaggccgg
      481 aaggaatact tggaggctga ttgtctggtg tggtaatttc aaagggctga ctaagtgctg
      541 acaggttgcc tgccgcatct tttgccctta ccgtcatcgt atatgttgat ccgcttgtca
      601 atccagtgat gtttgcatat gtagcggtta ctgacctggc cagatttcca ttgagatata
      661 catcatatcc tgtaacacct atgttatcgg ttgattcatt ccatcccagg gtaaagcctg
      721 tggctgtaat attggttgca tgaagatttg taggtgtact gggggcctct gtgtcggggg
      781 ctgataaggt ggtaacattc aggggagcac ttggttctga tatgttccct gctgcatctt
      841 tcgccttgac ggtcatcgaa tatgtacttg cggctgacag gccggtaatt gttgaggacg
      901 tagatgaaac tgagctcgtc agggtcccat tgcagaaaac atcatagccc gttacgccta
      961 cattatctgt tgatgccgtc caggttaagg agaagctcgt aggtgatata ttggacgcgg
     1021 cgagtccggt aggaatgctg ggggcctgta aatcgggagc atctttggtt ctgacactta
     1081 aagccgcgct cggatcagaa atgttcccgg cagcgtcttt ggccctgacc gtcatggaat
     1141 aagttgttcc tgcactcaga cctgatatgt ccgatgaagt tgaagaaact gtttttaggt
     1201 atgttccgtt acggtaaaca tcataccctg taacggcaac attatcggtc gacgccgacc
     1261 atgataaagt gaaaccatcg gaggtaatgt tgcccgaact aagatttgtc gggactgttg
     1321 gtttttctat atccgggtat tccttatcaa gatgccattt actcagactg tcgagtacaa
     1381 ccagttttgc cgcggttttt attctgttgg catcagttga attaagattg tagttgaatg
     1441 ttatttccat gggatctttc ttgaagatgg cagtatagaa acaacaggct gcagcgtaaa
     1501 aacccgcatc ggaaggatgg cttccgtcag gatgatatag ttcaatggac ggatagtttt
     1561 ttctgatgta acgccagacc gcacttacgg gggaaataga ggcattattc gactcagcca
     1621 taatcatgta tctttccctg ataagattat ccatcccgat ataggtacag acttgtggat
     1681 tttccggaca tctgtctgca tctccgtccc gccttcccca tgtcatataa aacattatct
     1741 cagcattggt gctgtatgat tgagcctcct ctttcaggaa gtgagcgtag ggatatacat
     1801 attcctcaac gaatgagagc ggttctgacg ggtatctgct gtgatcctgt aacacaacaa
     1861 tatcccatcc gccctgcctg atcaatgaga gtgtgtttgg ttcagatgaa tgccggtata
     1921 atgaatatcc tccgactgta attgtattac tgccataaat gagattgtca cctgttgaag
     1981 atgcaatatc atgtaccagc cagggaacgt tttcggaata actgttgcct atgaatagga
     2041 ctttttttca gctccatgga cagacaacag gggggcaaat gaaacaagta taatgataaa
     2101 cactagtgcc tttttcattc gcatatattt tatttgctta aggtcggata cctgtatacc
     2161 atccgggcag ggaacaaccc gtgattgagc gcgattatgt tcattcgcat ttgtattttt
     2221 gatcaagcgt gtggatttca tctgattatt atagagttaa taatttacga aaaaaaactc
     2281 aacttgcaaa aaatttaatt caccgtacct tctgacaagt gaaagtatga ctttaatacc
     2341 cctgttgcaa catcattttg accagcaaca ccaattcctt gacaagcacc ttttttcagt
     2401 tgaattatta attttcagga tgcccgatca ggcggttact atgataaata actatatata
     2461 aggttatttt aagaaattca cccctaagtg gttaatattt gtcctgattc ccgacatcat
     2521 ggaaagcaat tttcacatga tcaggaagga tcatagctgt ttgaatggga atttttggca
     2581 gctggattct gtttaccgtt atgggaaaac aacagggctc attattgagc cctgttgacc
     2641 gtgaaaacct gaaactgttt tttggatttc acagaaatta tttcccggca ggaatccgac
     2701 ttgctgagaa cctgaagagt accggtctct ttctgatgaa ggttctaatg acctgaatag
     2761 aaccggaaag tccgactgtt aaccttagtt gccgaacagt ctggcagcgt tatcgtaata
     2821 tatttttttc aggatatcct ttgacagccc gaagccgttg agagcccaat ggtaaccgaa
     2881 caggtttata tcataaaagt gttcgtctgc tgattccagg atcctgaatg tggtccggta
     2941 catttcctga tcgtatccca tatcggtgcc gtataataac ctgtcctgat atttctcata
     3001 aaatgcggcc atggttctgg gaatggttgc tgattcggca aatcgggcgg aaatatcagc
     3061 ataaagattc gggtacttgt cgaggagata tccaagtttc cccgggtcgt gattcaggtt
     3121 tgcaaaatga caggcgataa agattgtttt gggattatcc ctgactgcat tttccagtgt
     3181 ctggacaagg gcgtcgaatc ctataattcc gggccgggtc atatcgattc tccaggtctg
     3241 ggcgttcatc aggccgtcgt ttgttgagtc catcttttca tacatccata tcgggtcagc
     3301 cacatgtatg ctgaccggca ttttcagttc agcgcatttt tcgaacagtg gtttgagcct
     3361 ggggtcatca aaatgcattc ccctggcctg gaccggctca ttaaaggtga gtcccattcc
     3421 tttatctcct tcttctccta cgcctctggc tcccagctta taacaccttt cgagctccct
     3481 gactgcagca gagccataat caggctggtc ataaccggta tagtcaaagc cgcaccagag
     3541 ctggaaccgg tcactgtgcg ccgaatacaa attataaatt gaatcgaata gctttcctga
     3601 tgcctgggtg tgaacaactg tttttgcaat tccgcattca tccatgatct tcacccattc
     3661 ttccagctct tcactgttgg ttgcataatt gtgagagtgt gcgtcgaaca ccgggtaggc
     3721 agctttttca atatgcgtta ccggtatttt atacaatgat acaggccggt aatttttgag
     3781 caggatcccg tctgcctccc tgatccgtcc gggagactcc ggtccggggg aagaacaggc
     3841 tgatactaaa atgatcagcg atgcaagttt tgaaatgtaa gttttcataa acggatattg
     3901 ttaatggttt caagcttgtc tgtgatcagt tcggttactg catgggtggc agggggaaaa
     3961 attttccgca gatcgatttc atcgttttcc tcaagcagct ttctgagggt ggaaatatag
     4021 gtgtttttta tttcggtggc caggtttact ttacaaattc cattcaggat tgcttcctgc
     4081 agcatgggat cgggaattcc cgaacttccg tgaagcacca gcggaaccgg gacccttttg
     4141 cttattgcgg caagaagtcc gatatccagc ctgggttcct gcctgtagaa accatgtgcc
     4201 gagccaatgg atactgcaag tgaatgcact cccgtgtttt ccacgaaata aagtgcatca
     4261 tccggctgcg tgaagccctc attatcctgt ttctgaccaa gttttgcaac atagccaagt
     4321 tccgcttcca cgcatgcacc ataccgggaa gctatttcca caacatgccc ggtgatctca
     4381 atattttcag ctattggttt ttcactggca tcaatcatca cagaatcaaa ccctgcatcg
     4441 aggcattttc gtaccatcaa ttcagtgctg ccatgatcga gatggagcca ggcttcggct
     4501 ccgtattccc ggattgccgc ctttgccatt gatgatgcta catcaagtcc catgtattga
     4561 attgaacttt ccgagagctg aaggatgaca ggcagtttaa gccgaaccga ggccagaagg
     4621 attcccgaca gggtttcaaa gttgtagaaa ttggctgcca gaactgcttt tttgaggctt
     4681 ttcagctgat atagtttatc cttaattttc atttatacca aaggacgtcc aaacctgtca
     4741 atacttaact tgtttatcgt ttccggatcc ctaaacgctc ctgttcctcc tgccattgta
     4801 gtgttcagag cccctgccag tgcacctgtt tccaggcatt tcaggaacgg tttcccgcct
     4861 atgaaatccc tgatgaaacc tgcgtcaaag ctgtcgccgg cccccacggt gtcgataaca
     4921 ttgttgttta tgaaagccgg ctgatgaaca atatgggttc cgtcccatcc atatgccccg
     4981 tcgtttccgt tcttcaccac aatgagattt gcgtgatccc tgattttttc tattccttcc
     5041 ccgagtgagt tgctgtgtgt caggtatttg aattcctgca tattgggaag aaagatatca
     5101 acattgggca ataactggtc aagaggcaga tcccattttt catccggatc ccactgggta
     5161 tccagcgatg tagtcagccc gagctccctg gctcggctga aaagggaagg cagatctttt
     5221 ttaatgcccg gctgcatgaa gcatgtggca aaatgcatgt gtttcgcccg ggaaagaaaa
     5281 ttgaaatcaa tgtcggcaat tttgaaatca tacattgctc ccggataggt tatgttggcc
     5341 ctgtcctgat cgtataccag gacgattgta gccccggtgt tgagttttgc ggaactgatg
     5401 atacgggatg tatcaacatt ccttgcactc agtgaatcga ttaccaccct ggcaaaatta
     5461 tcttctccga tcttcccgat gaaagcgaca cttgttccaa gggcactcag attgctcgca
     5521 aagatggcag aagaggagcc cagcgtcact tccatgtaac cggccatgag ttcctttcct
     5581 acctgcggca gatttccgat gtcgttgagt attatgtcaa cgttcagttc tccgataaca
     5641 catacatcaa attttttttc tgaagcaatc atatgatgaa acaagttgac tgaaatccgg
     5701 atcagtgttc ctataaaaga gaattaagac caacaaggtt gaatttccag aaatatttat
     5761 ccatggcact ttcaatggcc gattcgacaa ttgtggcggc ttctgttccg ctgccggcaa
     5821 ggttttcata aagctgctgc cttgccgaca caaccgcagc ttcttcccag atatagcggc
     5881 atcctgtttt aatcagccat tcctgtctct ggaaggagat tgaataaaag tcgtcgggat
     5941 tttcattttc ctgaacccat tttttccacc tgcctgattt tattaccgca tcccaaaggg
     6001 tttttttgat cggaatctga gttttcaatt ttccttcctc catcaatgcc tgttggatgc
     6061 tttcgagttc catcaatcct tcgtattcaa gttctgtaaa ttccggaccc acgtttgctg
     6121 cgcccatgcc tgattccggg taggcttcag gatttgtcac attatcggaa taatgtccct
     6181 ttatcaggct gccgtattca ccggcgatcg ttgcaagcct gcgggctact tccggatcaa
     6241 aggttgtggt atggagatcg gttcccactt tgccgacgac aaagcagggc cagacatcct
     6301 taaggttgtt ttgttcaagt cccattttca gaagccggag aaatttcctg aaagtgctta
     6361 tatctgccag cccgccatgt acttcttcag tgccgacttc gtaagatatc ggcggcagtc
     6421 ctttactttt cctgaatgat tctgcatgct ttatcagttc aacagtcctt tctgcaacgg
     6481 tttctatgcg gatcggactg ttttcctgaa gtgtgatatc aatggtggga tcgacatgga
     6541 tgagatcata tcctgccacg atggccgctt cgaatgatgt ctttactgca tccatggttt
     6601 catcgtagct ccattttccg gttctgtgga tatcctttag ccagggtccg ccatgatcaa
     6661 ttgcaatgat gaccggagat gttacattca ggttccttgc atggtggcgg accgtttcta
     6721 caaactccga cggagttagc ccggtatagc cgccgtcgag atcaacctgg ttgagggtgg
     6781 cggcaaactt gatgggtgcg ttgcatcttt tggctgattt cagggctgcc ctgataactg
     6841 ataatgaatt gggacaggca gcaaaaatcg ttcttttaat tccggtctca gactgaaggg
     6901 cttttatcct gcggaggatg tggtccatca ggggtatacc ttctgctgcg gctctttccc
     6961 taagcttttt gttcattgag gttgatcaga atttgtatat ggttacgtcc tccactatcc
     7021 tcgatattgc tccgctcttt gacgggttat cgggccgcag gcccagcgca agtgatttgt
     7081 aatagccgag aagctgcgcg ggaatgacat tgcatacagt aaggaattct tcatccagtt
     7141 gatttcctga atcggaaagt tttatgtcga gatcgaattt cagtccgtct gctgagcttt
     7201 cataaatacc gatggtccgg agtggttttc ttcccttttt catggaaagg acgagatctc
     7261 tttcatactg cctgacataa tcattatttg agaaaatgaa tacaaccagg gtggtttcgt
     7321 cggttactgc tttgggtccg tgcctgaatc caaggtaggt ttcattcttg cagattatta
     7381 ttccgtcggt cagctcctga agcttaagat gagattcggt ggcagtgccg aaaaatggtc
     7441 cggaaccgag aaaaaccgcc cttttgaaat cagctgatgc gatctccttt attttgccgg
     7501 agtaattgtc gatgatttta ttaccgtaag aataaagctt attcacctgg gaaccgagag
     7561 aatccatttc cctgagtcgt gcgataagaa taccacacag gagcattccc gaatagcttc
     7621 ctgtcatggc aaggcctctg tcatttgccg atggaggaag cagaataaca agcttttttg
     7681 attgggtatg gtaatttgcc agtcgcccgt tcccgtcgca ggtaataatg aggtgactgc
     7741 atcttttgca taatttgtcg gccagttccg ttacggccgt actttcagga ctgtttcccg
     7801 accgggcaaa tgacaccaga ataattgact cgtcattact gaaataattc tcaggatatg
     7861 aaagcagatc ggttgttgcc accgaatcgg tacggatcat taaatgcctg ctgaaaaccg
     7921 gactcagtga caaaccgata aatgcacttg tgcctgcccc ggtaaggatt attctgtcag
     7981 tgcatgggag cgatcctgta aaaaaggaca taattctatc cttatctgct gatatctgag
     8041 aatatatttc cagccaaaga tcaggctgtc ctgcaatttc acctgcagta tgcacagctc
     8101 cgagctcttc aagcagagag gtttctgagc cagatgggaa catatcttgt tttattattg
     8161 attttcctga taatacaaag atattaattg tgaaaatata aacaaattta atgaaagata
     8221 tttgtaaaaa ataacaagta aatgaaagat caaatttgtt ggttcagaat agagcttcaa
     8281 tattctgtaa cctgctgtaa aaacctgaga tgtatgcagg tttttaaacg ctatttaaat
     8341 atgatgcggt atattgattc ttaattattg ttgattattt ttgagttttt gcaatggaga
     8401 ttcctttgta caactaaaaa ttcagaacct gaattattca actttaatat ttaaaccatg
     8461 atcaaaaaga atttcatgtt tttatttctg gttctgtccc aggcgcttcc cggacagaaa
     8521 gcttccataa ccattgaaac cagggatata cttacctatc ctttcagcga tccaaatccg
     8581 gtacccatac tcgtggaaag aagagacgaa atatatccgt atcattcatt caatggctat
     8641 agcctgaaag gcagaatgca gaaatggaac gtcatcaagc tggagaatga ctatattgaa
     8701 gtctgggtga tgcccactga cggagggaag gtatggggag caattgaaaa atcgacagga
     8761 aaggagttca tataccggaa cgaagtaatg aaatacagga atatttcgat gcgcggacca
     8821 tggacatccg ggggtattga gtttaatttc ggatatatcg ggcacaatcc ctcaacatgt
     8881 gtaccggtcg attatacgac cgttgaaaat cccgacggca gcgtaagctg tattgtcgga
     8941 agccttgacc tgccttcaag aacgaaatgg agtgtggaga taagggtacc caaagacaaa
     9001 gcttattttg agaccaggac tttctggaac aatccgacac cccttcccca gtcatattac
     9061 aactggatga ctggtgcggc cgtggtttcc gatgatcttg aattctttta tccagggaac
     9121 caggaaatag gacacggagg agaacatggt ttatggcctg tggacaggga aggtcataat
     9181 attgcattat acaggaacaa taatttcggt tcgagcaaat cgtaccatgt ggtaggcgag
     9241 ttcaacaatt tcatgggagg atattaccat aattcccgct ttggattcgg acactgggct
     9301 ctttatgaag aaatgcccgg gagcaagctt tggctctggt cacttgcaag ggacgggggt
     9361 atctgggaag atctgctgac agacacggat ggccagtata tggaatttca ggccggacgc
     9421 acgcataacc aatacggggg gacatcaaac aagacgccta tatcgcaggt accgttcgca
     9481 ccaggactca ccgacaggtg gactgaaata tggttcccgg tcaaggaaat tggcggcata
     9541 tccgatgtct caccgtcagg agtgcttttt gcaaaaaacg aaaacgggac actccagttt
     9601 ggagtcaact cctttgcctt tgccgaagga acaatcatta ttgagtcagg ggggaatgtg
     9661 atctacaggg aggatgtcaa cttcaagccc atggatgtgt tcaggagctc aattcccctt
     9721 gctgccggga ccgaatacga gatctcggtc aggggaatgg ggcttaagta taatccggcg
     9781 aaaaagaaat tcctgtcccg tccgttcatt tcaactatgc cgacggacca ggtaacggct
     9841 tcatcggttt accaggaagg aatggagaac aaggagaaca ggaattataa agttgcgaag
     9901 gtgcttctga aaaagtgcct cataatggat cccctttaca ccgatgccat ggcggcgctt
     9961 tcagagctgt acctgaggag caatctttac gattctgcgc tttattatgc aaataatgcc
    10021 ctccagatgg atacatatca tcctgcaggg aattattttg cgggattgac ttacaaggca
    10081 aagggcaacc ttatcgatgc cgtggaatcg ctcggatggg cagcaagatc aacacaatac
    10141 aggtcagcgg cctatgcgca gatggctgcc atttacctgc agcttggtga catgcagctc
    10201 gcagatcatt atgccggact ttcgcttgat tttgagcgta ataacttcaa cgccttgcag
    10261 gtactggcca taaggtccag gaaaataaat gccagggaaa aggccgagga gtatctgaat
    10321 actatcagct cccttgatcc tcttaaccat ttcgctgatt ttgaaagata ccttcttgat
    10381 ccttcggcag agaacgaaaa gcttttcagg aaaacgataa ccaatgaaat gccttaccag
    10441 acctttctcg aactttgtat ggtttattat gggatgggat tcagggagga tgccattcag
    10501 gtgttggaca aagcaccgtc gcatccctta ataacagtct ggaaggcata tttaaaggat
    10561 gatgcagcaa ttctccaaga agcagccgca gcatctccgg cttttgtgtt tccctacagg
    10621 acggagacag tgtcggcact tgaatgggca gtgtcgaaaa acaacagctg gaagttcaag
    10681 tattatttgg cacttaatta tgcagccatt cagcgtgatg acgatgccat gaagctgttg
    10741 cgcgagtgcg gatccgagcc cgattatgcg cctttctact taacacgtac ttcattgttg
    10801 cggccgatga ccaatgaaca aatgctgact gatctccagg ctgcccggag gctcacaccc
    10861 gatgactggc ggactgccgt aagactgatt gactattatg aaagaacggg tgacaacaaa
    10921 aaagccctgg aaattgccac ggagaccttc aaaaaggaca gggaaaactc gatagtggga
    10981 atacgctatg cacaggcact tattaacaac ggactttatg ccgccagtct cagggccctt
    11041 gaaagcatga ccgttcttcc gaatgaaggc gcgagtattg gaaaggttgt ttatgagcag
    11101 tcatctcttt ttcttgcaat ggatatgatg ggccggaaaa agtatggcga ggcgctaaaa
    11161 tatattgaaa agtcaaaatt ataccctgaa aacctcggtg tcgggaagcc atatgaaccc
    11221 gatacaagga tccaggatta cctgaagatc aaatgccttg agaagctgaa ccggaaaaat
    11281 gagattcccg aacttacaag atcggttgtc gattttacaa aaaaaggaat cgatcatccc
    11341 tccctgagta gtttgcttgc aatacgcctg ctggagtccc agggtgacag ggcggctgca
    11401 aatgatcttg ttcagcagat caggaattct gacagggccg gaaatccggt caacaggtat
    11461 gttcttgccg cagcaggaaa tgaccagtct gtccttgcag atcttgaaaa aagaatggcc
    11521 ggtaacagtg tgttccagat attgaaaaaa gtggaggagg tcacaaaata aggatataat
    11581 aaacaaataa acatacaggt ccgcagaccg ccgtatcgat gatccgggcc gattttgcgg
    11641 acctgtggtt tttaccggga gaagaggctg gaaacaataa gggtcttcgg ggctgatcct
    11701 gggttaacag ggaaggccgg caataagtat atcatttatg cgattcaatc agctctgcaa
    11761 gttctttcct gtattcctcg aacttataac ccctgtgcgg ccagcttgca tcatcaagat
    11821 ttgctgcatc ccacactccg gcgggaatgc atttatttac tatgacctca atatcttttt
    11881 ccagccccat ttcccgggcg agccacaggt attcaaaatc ctgggctccc ctcctccagt
    11941 ttttggccct gatcgacgac aagggaccat caaggcttct gtcctcttca ggaaaaagtg
    12001 cgtcccttcc agggtacagg aaggttccgt cgccgttgat cctgtcttca taggtaagcg
    12061 gattcacgaa tacattcagg tttgtccaga aagttgttga ccagtaaaaa tactggtcga
    12121 tattgtattt ccaggcaatc cacggcatta cgcggaattc gactgcatcg gcatcgctca
    12181 ccacggctcc catgcccgga cggaacccgt tatagattcc aagtttgcgc cctgcggccc
    12241 tttcttctgc caccattccg gctgtaagat tcagggtcca ttcctgggcg gagattgacc
    12301 agaaatccac atgccccttc aggccgggta tgattttatt tgtaaccagt gccgggatgt
    12361 tcttgccggg acccggattc gtgtgggtcc agccggtctg catcctgatg gtatccatcg
    12421 atcctgttcc cttggcacct gcaggtccct tgaaatctgg ttcgtcagga aacaggtatt
    12481 tatgcctttc cacataaagt gcatttttca ggaaccagct ctcccatgca tcagagcctg
    12541 tccaccaatc ctttttgttc atcttcctta cgctcccgcc gtattcggcc ggaaagcctc
    12601 ctccgtagcc tattgaaaac gtaccgttgc caatgtttct gcccggtccc caataaccat
    12661 tttcctcggt ataggcatca ccggtgagat accttttata atgattatcc atttgattaa
    12721 gattgtttac cgggcagacc agatcgaacc tgtggcgatg ggcaagctgg ttgtatttaa
    12781 gctcgatgct gaaatattcc tcgctgtttc gttgaacgtt gtggcgctca gcaataaagc
    12841 ccgggtagaa gccaaacatg ttcctgagat gggtagtgtc ggaaagcgta aaatcatata
    12901 ccttaatgaa taaaggtatg gttttatatt ttttccctga tacggttact gtgacctttc
    12961 ctgaataagt accggccacg gcattctccg gtaccaggat atctgcccac acagcctgat
    13021 tcatttcagg ttcgatactg aaaggagccc ctcctttttc cggaggtgcg gaaaacggga
    13081 taaggcagtc gggaacccag cccgtatagt atgatgaagg gacagcagtt tcagagaaaa
    13141 accacagcgg gggtgaccgt tttatcatgt taagataatg ttccgtaaac aattcgacat
    13201 atttacccct gtaatcgaag ggatccgaag gagatgcatc cgatccggga atcaccgaac
    13261 tgccgttttt caggtcgctt atgcttacat tcacctgttt ggctccggcc ttgcccgcct
    13321 ggattatcag ctgaaaagct attatttcat tcctggctgc gaagaggctg attgtattgt
    13381 tcttccaggt ggcattcgca ggttcggagg agatgggatt tgagatatct tcctttttga
    13441 tcttttctcc gtcgtcaact gcccagatcc ttttaatgcc cggatccgga ctgcaggatg
    13501 aaacaagcag gatcagcaat atttggacag gcaggtacag gaccgctgat ttaatgtggg
    13561 atgtaattct cataatgatt gttattctgg aattgttaaa aagccggata taaaatgtta
    13621 cgggatattt ttcagtgatt tcatttcacc tccaggtttc ccacccgata ccacccgtcg
    13681 gttgttttgc ctttgtttgc aatggggatc acgtggtcag gtgaactgtg gaacacaatt
    13741 cccagttcga gggtatacgt tccgggttta atgtcaccgg gaagcttgta caatgcttcg
    13801 gctttctttt caccgggaag ccagccacgg attgaaacat cggttaccac tgctgcctga
    13861 attttgccat ccgtatttct tagccggagt gcaattctgt ggtcgcggta cgggggagca
    13921 atcccgaggt tttcccattt catggaaaca ggtattcctg cgccctgaac cggggcttta
    13981 ttgtaatcta tatctctcag taccagcctg aaaccaatta ccttcattaa cctctcaatt
    14041 tctgggagcc agtttttggg aataaagtgc gatttattct ggatgaatgt ggcatgccat
    14101 gcgattgcat cgtcgacaac gcttttcggt ggtgtgggga aacttccgaa tgtgccgcat
    14161 ggttcaagag cgacagttcc tgttttccag ccatcaggca gcattttgac cattggccag
    14221 taggaatccc tgtgatgatt ccagcccacg ccgggaccat ctccgttgcc ccagcagtcg
    14281 cctctccagg ctgacctgcc ttttaagctg gcatacagca cattgtcctt gtcatccaca
    14341 agtgcggtaa gcggggtgtt aggaaatgtc tcgtaataca ggtcgatgat ccttttccgg
    14401 gtcgcatctc cgggcatcag cttcggatcg cagtaattgt gccattctcc ccacagtccg
    14461 accgatccta tatccacaag ggccaggtcg ggatgcccgt cgtatttttc ccccagggcc
    14521 cttatcagct tttcatgata atcccacaca acctgctctt ccaggtcaac ctcataatga
    14581 gcgccttcct cgggacattc ggaataggta tacctgattc ctttgttcag aagccattgc
    14641 ggaatagttc cctcatgacc gggccatgaa agcatgaccc tgaaggcaag ggcctggttt
    14701 gcctccctgc attttctcag aacctcatct atcattccga aagcgtattg accttcggca
    14761 ggctcaagat ctccccatgt ccagcgaaaa taagcacaac ccgacctgaa ggggagtccg
    14821 ttcatggaag gatctttatc tgctgttctg aaaaatgtct gccatcccac accgggaatc
    14881 tgaagaaagc cggtatcttc ctccagtaca aaattttcaa tcttctcatt tgatgagcat
    14941 gcgttaagta tgcccagcgc aagaataact gataaaatgg ccgataaacc ggtaatatgt
    15001 atttttttca ttgcagatca attggatttt gattgatgtt tgctgaacca tgtttttacc
    15061 tggatcagcc ggatcatttc ttcccgttgt tcagtcttgc aatttcaccg gcaattttcc
    15121 cgcggatcat ttgaatcttt tccgggtcac tcgacgacga ccaccagtct ggcgcaattt
    15181 cagagacgag actggtgact gtttgcctgt cggtaagctg ttcgagaagt gcgaaatact
    15241 cccagtcttc cattgaatcc cggatatttt tcagtcttat gcatgatatc gggccgtcga
    15301 ttccgcaggg tacgccggga tacaacaggt atccgccgcc gttgaactga ttttccgatt
    15361 ccgaaaaggc gggcagcagc cagggatcca ttattcctgt acgagattca ctgatttgaa
    15421 cagccgacca gtacaggagc ccggtgatgt tgtactgcca gttgatccat gtcggggtcc
    15481 ggtatgaagt aagaagggca tcaatgtgcc agtaggggga atcaaagtct ttcagttttt
    15541 catactcagg atggtacgga ggagttctct gtgagagtgc ggtatatgac cagacctcat
    15601 cgccatgggc gatcttttcg tttattgatt cacgatcgat aaatgcccac agcgggcacc
    15661 atatatccac tgccggatcg atgtcgggcc actccggatc ctgcttgtag gtctgttcta
    15721 ctacaagaca tctgaggtct ggagaagctt catggatcat ctgtcccagg gccagcactt
    15781 cttcgtagtt ctctttcagg ttgggttcat caagcatata aacataagcc cttttatccc
    15841 agccgttgtt ctttacgtag ttgtaataat ccctgtaata gcgtatggct tttgcccggt
    15901 ttgcggtagt catgtcagaa aaaccggggc caggaatttc aaagtcggtg acatggaggt
    15961 cgcggatgaa ttttgagagg gcctcatggc ggaggggatc aattctgagc gaaccatcgg
    16021 ggttcacttc gggcataagg ctggacggga tcgggggatt gatgcggtga tcggccagca
    16081 tccggcaata ccgcagctca atatcacggt ttctttccga attccctgcg gcaccgaaaa
    16141 gccgcgggat ctgccagtta acccttccaa agtgattacg gtgagtaggt ccgtcaggca
    16201 gggtgaaatc ccatactgtg agtttaacgg gcagagagag cttttttgtt ttcaggtttt
    16261 cagcggactg gccgtgcggc ataggataat tgtcgaaggt aacggtaaat tctccggtat
    16321 attcacctgc cggcgtttca cgtggtatat aaacatcgat ccatataggc tggttctgac
    16381 ctttccacac atcaaacggg agggcataca tttcaaaacc gctggaaatg aaaggagtgc
    16441 cccacctgac cctgtattga ctgaatggtt cgatcggctt tccggtttca ggatcaatga
    16501 acggcaccag gggatcggga taaagtccgg gaggcagctc agccctgggt gatgaccgtc
    16561 tgacccttgc atattcagcc ctgaacaggg tgatgttttc cctgccgaca gaagcggttt
    16621 tccctctgag gtcggaaatt tcagcagcga caaccctgat attttttgta attgcaccaa
    16681 caacaacctg aaatgattca acttcattcc tggcaccgga aatcagggcc tggtcactgc
    16741 cagacaacgg ctgatcctgg ccgatacgtt ccatggaaga gatagagaca agccggactt
    16801 ccgtttcctg cctggctcct gaacatgaag ccagcagcat ggcacacagg attgcagagc
    16861 caaccaaaga tgttttgttt ttccgtttca tgtgagaaga aattaatata ctactgaaga
    16921 ttaagtggtt cacatggaac ccaagtgact tttattttta tgcttgtttg tgattttgta
    16981 tcatgtggtt ttcatattac acagtttttt ctgtttatga atagatgatt catttattca
    17041 ttttaataga tattatgtaa gaatatgttt ttgacaatag taaaatgctg aaacttacaa
    17101 tataataatt aaatccggta atttgagatt ttttaatgtt tttagcataa tttaattatc
    17161 ataattaaac aaaagatttc tccctttttt taaataattt atttgtttca tataattttt
    17221 attactaact ttattatttg tgttaaccat ttctggttcc tgatcgtatc agaatgatat
    17281 attgaacaga cctgaaacaa ataatagttt aaccgtaaac cttaaatctt gaaacgcatg
    17341 aataaagaat tcagatcttg ttttttgtcg cgtttagcgg ggaaagagag tccgcttaac
    17401 agacgcatta ttgtagtcag cctgctggct gtcctgctgg tcagcctgcc gcaattgtca
    17461 agggccgggg caggtaaaaa tcccggcagt aaaaatgaca attccgctgc cctgatcatg
    17521 cagcagagag tgaccggaac catcactgat gcttcaaccg gcgaaccgct tgtcggagtg
    17581 aacattgtcg ttgaaggaac aacaacaggc acaatgacag acatcaacgg caaattcacg
    17641 attgaagctc ccagccggaa ttccgttctt tcaatatcct atatcggata tgtgtccctc
    17701 aaggtaaatg tggagggcag ggctatcatt gatgtttcaa tggaatctga tctcagggca
    17761 ctggaggaag ttgttgtaac cggttacgga acagtgagga aaagtgatct taccggatct
    17821 gtcagttcga tcaagaccga gcagatactt cagcttccca cccagcgtgt cgaccaggcg
    17881 atccagggaa gggcccccgg cgtgtttatt ttaaataccg acggttcacc cggaggtaat
    17941 accatgatcc gtatcagggg cagcaactca atcaatggcg gcaacgaccc cctgatcatt
    18001 gttgacggcc tgcagggagc caatatcaat cagctgaatc cgaacgacat tgcttcaatg
    18061 gagatcctta aggatgcttc agccacggct atttacggtt cgcgcggagc aaacggtgtt
    18121 attcttataa caacaaaact gggaaaaacc ggaaaaccag tgattgacgg gggttacagc
    18181 gtggggatcc agagactggc acgaaaactg cccgtgatga atgcctatga ttttgcgagg
    18241 tacaataatt ttcagaacag cttcaacact gcctatggac agaccccgaa actctacttt
    18301 acggaagccg acattgaata ttataaaacc cattccaccg actggcagga tgagatttat
    18361 aaaaccggta taatgcagac cagcaacctg gcaataagcg gtgcgacgga caggttgaaa
    18421 tacatggttt caacgggata ccttgatcat aagggcatcc tgctgaattc agcctataac
    18481 agactgtctc tcagggccaa cattgcagcc gatattaccg actgggttga tttcggaatg
    18541 aattactcat acacatacga aaaatacaaa tcacccccgt ttgaggccaa ccttttcaca
    18601 tcggtgaatg acgcgccgag atgggctccc accgaaccgg tttatgacga gaatggcaat
    18661 tactggaaac atcgcgcggg atatgctgca tatgatacct ggaaccccgt ggccaccgca
    18721 ctggaaacca aaatagacaa cccgacctac cagaataacc tgaatctgtt ccttaatttt
    18781 aagctgttaa agggtcttac cctaaaaatt atgggaggag ggacctttac atcgtcatac
    18841 aatctcaggt atgaaaatac caagacacgc aacggatttg cgaataacgg cgttggaaac
    18901 atcactgata acttccggaa cgcttaccag aatacaaaca ttctcacatt cgaccggact
    18961 ttcggaattc accacattac cataacaggt gttaccgagg agatcttcag tcgttcgatg
    19021 ggcagcagca tgagaggaat caatttcctg gttgaccagc tgggcaccga caacatggat
    19081 ggtgccagag cagtgacggt cgattcatgg gcatcaaaga gggttctgat ctcatatatg
    19141 gggcgcatca actacggcct tctggataaa taccttttaa ccctgagcta cagggctgac
    19201 ggttcatcag tgttcggggc aaataataag tggggttatt tcccgtcagg ctccgttgca
    19261 tggaggatct cacaggaaga gttcctgaaa gactctgaaa ctatttccga tcttaagctc
    19321 agggtgagtt acggcgttac gggtaatcag gctataagtc cctttggcac actcgcaagg
    19381 cttggttcgg gctacaggta tccatacaat aacggtgata atctcaatat cgggttcggg
    19441 ctttcaggca ttgcaaatcc caatctgaaa tgggaatcaa ccggccagac aaatattggg
    19501 atcgatcttt ctcttttcgg cggacgactg acttccacaa tcgatgtata caggaaagtc
    19561 accaagaatc tgctgatgcc caggcagctg cctgattatg taggggtgtc aagcgttatt
    19621 gacaatatcg gatcgattga gaacaagggc atcgagatcc tgataggcgg cgacccggta
    19681 gccggacggt tcaggtggaa cacaagtgtg aattttacaa tgaacaggaa caaggtgctt
    19741 gatctcggac cgggagtgac aagaatagga tacacgccca catcgggagg atacagcctc
    19801 ggtaccgagt acatgttcct tgaggtaggc cagccctttg gccagatgag aggctggaaa
    19861 ttccttggac tctggaggtc ggacgaggaa gcagaagcaa gaagctacgg ccagctgccg
    19921 ggaacgccga aatacgcgga tataagcggt cccgatggtg ttcctgacgg ccaggttgac
    19981 ggtttcgaca gaaccacaat tggctatggt tatcccgatt tcaccgttgg ctggaccaat
    20041 atattcactt acaagaacct ggaactttct ttccttatta acgcctcata cggaaaccag
    20101 ctgttcaata cgatcaggat ccgcagggag tcaagctggg aaggaaccga tccgaaggta
    20161 tggaattact ggacacctga aaaccaggat accgatgttc ctgccttgta cgacggccaa
    20221 tgggtgctgg accagcacct ggtaaacaaa tatatcttcg gcggaggatc cggagccaca
    20281 tcgcgttggg ttgaagatgc ctcttttatc aggctgaaaa cacttacgct ggcttacaat
    20341 ttcgattcca accttctgag gaagatcagg ttttcgaaag ccaggctgta tttcaccgga
    20401 acaaacctgc tgactctgac caaatacacc ggttatgacc cggaagttgc ccagtacgcc
    20461 tataatgatg cactgattgg cgttgaccag agcgtttatc cgcctgccag gatgttcact
    20521 ttcggtattg attttacttt ctaaacctaa tcatcactaa gaatatgaaa aggaaatttt
    20581 caagtacact aacaataatt ttgttgtcag cattgattct cttttttgct tcaagctgtg
    20641 atcagctggt cgagaaaccg accgattttg tgggacctga caactttttt acgacacctg
    20701 cgcaaatcga atcagctttt acagcatcaa tggacgtcct gttcggcgga tggacatttt
    20761 acgactggta cggatatcct gagatctttg aaatggacga ccaggtatat gggggcgacc
    20821 ttgttatcgg cgacgatttt ggcagtggat gctggcaaaa gcacttcagg gcgatatcgt
    20881 ttatcaatcc tgcaataaaa gccctgaatg ccggccaggt aaccggtatc tcccaggaaa
    20941 aggctgatga actgatggcc caggcgaaat tcctcagggg atggaattat ttcatgctgg
    21001 tacgctacta tggcggcgtc cccctgatag aagagacaac cgatgccacc agggatgata
    21061 taccaagagc ttccgttgca gaagtttatg atctgattgt aaatgacctc ctgtttgcaa
    21121 cccagcatct tccggtcaaa tggggagagg aaaacaaggc gcgtccggca gcggatgctg
    21181 caaagactct tcttgcaaag gtatatatta caatggctgg ctttccgctc aatgaaactt
    21241 ccaattacgc gaaggcaagg gacatggcca agcaggtaat tgatgcaggc aactactggc
    21301 ttgtcccgga cgttgaggat gttttcgaga tggaaaatac ctatggacct gaattcatgt
    21361 ggtgtttcca taattcggag gaagagtatg tcattgaacc tcagatctgg ctgccgggcg
    21421 aaatggcagg gggctggggc gataccaggt gcgaaaaaag atggggcgag gccttcccgg
    21481 aagaacccag gaagcatgca taccttctcc ttgaagatcc atggttcggt accggaacaa
    21541 catatctcga gtggggtggc agcactccct atgtgaggaa atatctgtat gactcgcccg
    21601 aaaacatgga aaagaacgtt tcatatcaga gcatgccgat cctgcgatat gctgacgccc
    21661 ttctgctgtt tgccgaagcc gagaaccagg tgaacgggcc aacgcaggca gcctgcgttg
    21721 gcccgttcac ctggttctcg gcttcggcaa ac
//
LOCUS       DAOOYG010000036        21615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2275_length_21615_cov_8.701716, whole genome shotgun sequence.
ACCESSION   DAOOYG010000036 DAOOYG010000000
VERSION     DAOOYG010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21615
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_21615_cov_8.701716"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..918)
                     /locus_tag="PK288_04920"
     CDS             complement(19..918)
                     /locus_tag="PK288_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPM18295.1"
                     /translation="MGTTRRSFIAKSLFAAAGFSAGFDIKAIDGHKTEDSNFNIGSLS
                     SPDPDAFRISIFSKHLQWLDYNGMAGVLSDLGFDGADLTVRPGGHVLPENVEADLPRA
                     VEALAKKGKRVIMITTSVTDAADPLSEKILKTASSLGIKHYRTGYFHYNDRNAVSGDL
                     ARIREQLEKLANLNAKYSISGEYQNHSGDYGQGIYFGAPIWDLAAILEKINSPWLGSQ
                     YDVYHATVEGANAWPVGLRLISPRVRTIDIKDFMWVEKNGKTISEPVPLGQGMVDFKK
                     FFSLLRMHDIRVPFSAHSKFFCS"
     gene            1204..2682
                     /locus_tag="PK288_04925"
     CDS             1204..2682
                     /locus_tag="PK288_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biosynthesis protein"
                     /protein_id="HPM18296.1"
                     /translation="MIKLNLSVIGSVIFATATFIAGCNGIDIRDIQPANRQPAIEPDY
                     SGITIPYNIAPMNFRIGEEGKKFLVRISSSNGICNKIRSSDGNVIFPLKLWRALTGNN
                     ADSIEIEIIAVNKDKKYIKYSPFSMKISGDSVDPWLCYRFLYPGYETWVEMQLILRST
                     ETFKEKYFIENQLLERNCINCHSFNSKKAERFLVQVRGSKGGTYFHDGMDLTRRILRT
                     DNMKANVVYPSWHPSGKYVAFSSNRTIQSFHMKAGKNIEVMDLFSSLLLYDPDQNLIS
                     PVSDRDTANYMETYPCWAPDGKYLYYCRAEQVMEGFDYRKIKYDLARKRFDSDTGEFG
                     PAEVIFSASEINKSVSFPSISPDGRYLVLTLHDYGTFSIWHREADLYLLDLQNMDIKK
                     LPFNSNETESYHSWSSNGRWIVFSSKRLDGLTARPFIAFFESPGKTGKPFVLPQKDPT
                     LYRRLDKTFNRPEFVPALPASEARDFMKASAKPPLKARWIEK"
     gene            2679..4469
                     /locus_tag="PK288_04930"
     CDS             2679..4469
                     /locus_tag="PK288_04930"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HPM18297.1"
                     /translation="MTRKNLPVLILFITSFAYLYWFAAGIFFYQEQKSLFIFSYEYLA
                     KHLVKPGGLLKYLAGFLTQGYLSDLYGAFINSLIIALLAILLRSVAERLSGSRSFTMP
                     LAFLIPLGLMICQSNYHFNILHSLGFLLAAVLFLISLNLTRKTFHVILIFLFPVFYFM
                     MGTYSLIYLCTYLIHSILYQNNRYGRIFTLLQIFISIVTLVLFYKLFLYQPFSVITGY
                     PFISNEYGRANGLLYGVTVLFVLLPGLIKISDLPGSEKHATAKSTVIAACLYGISIIV
                     LVRQYDPVFEGIMRTEKRYCSGKFDEIIVHHEKYPSSNIIEQFYYNLSLSEKGKLCDR
                     MFFGCQDTGPMSLSLGGNREQASRTMHYYYAVGLVNEARHLAFELMVINGYSPENLKM
                     LIKTELINGNFRVAERYLNILKKTLGYRTEAGRLGKMLENTELVNTDDELGEKRKLMP
                     KDDFFIHTEDAMNIDLLLKSNPANRKAFEYKMARLLLEKDILAIAEESVKMADMGYTT
                     IPRHIEEAIVAFASYKKALPDLAGLVLSAGTNARFLNYVRSVNSFAGDRAAIKKSLNR
                     AEKDTFWYYLQFGQIRGDFTRSRPVDRNVY"
     gene            4591..5283
                     /locus_tag="PK288_04935"
     CDS             4591..5283
                     /locus_tag="PK288_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18298.1"
                     /translation="MRKTTFLFFLFAAVSLSLSAQEDKLITVKAGHKILDYFPDEIRY
                     FYPEFRTGRVVFRNGIYSERLLNYNFLSGEMEFLQSRDTLAIINKKDIRYVGIDRDTF
                     YYDKGYIKQWARGEKVIGMKERFELKEIQSRDSYGTSSAGASTNTYGSMPADGNFYKL
                     SANKDMVFQRTRQFYIADPDGPFVILNRKSLLAAYHGREDKIKQFLKTSRIKFNSPPD
                     ISKLVDFLNKLD"
     gene            5296..6897
                     /locus_tag="PK288_04940"
     CDS             5296..6897
                     /locus_tag="PK288_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPM18299.1"
                     /translation="MHIETNRLVFPLILAGIFLTGCVQKKNQQKVELKMLQDEKWWGG
                     AVTDGRKSPYAERNFYYDQLGDCRGNQAQPLFLSNKGRYIWSEKPIEISISGNIISAG
                     AHGAEISTGTSGKTLKDAFLYCSRTFFPPSGSTPDPLMFTNPQYNTWIELQYDQNQTD
                     VIRYAEDIIKNGFPPGVLMIDDNWQERYGTWEFDCRKFPDPASMIDKLHRLDFRVMLW
                     VCPFISSDSPVYRDLQGKKLMVFRDKEKTAPAIVDWWNGASAIIDLSNPEGAAWFREQ
                     LDNLAEKYGVDGFKLDAGDPEYYTGTYSFGDSSPNEHCESFAKIGLGYPFNEYRACWK
                     MAGQPLAQRLRDKSHTWADLQVLIPDMIGLGLTGYQFGCPDLIGGGEWTSFQDSSILD
                     EELIVRSSQIHALMPMMQFSVAPWRVLSKDNLKICREMANLHLSMGGEIYELAKECAA
                     SGEPMIRNLEYEFPGKGYENVKDQFMLGKNILVTPVVLKGQRSREIIVPEGRWQGDDG
                     TTVTGPVKIIIDVPLERLPWYRKRS"
     gene            complement(6898..7614)
                     /locus_tag="PK288_04945"
     CDS             complement(6898..7614)
                     /locus_tag="PK288_04945"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPM18300.1"
                     /translation="MILVGTFCSFSEAPVTDDAPVYHVGKLKKPMKIDADWNKAQWKN
                     VRSINITNYMGTVPEFKPETQVKMLYDNDYVYVIFHVKDRYVKCITDRINGPVWQDSA
                     VEFFFAPDPRLPLLYFNLETNCGGTPLLSYNLVPRKESKRIDLDDIKKVEIASTLPKI
                     IDPEISNPVEWTLEYRIPLDVLEKYSNAARPQKGVEWKANFYKIAEITSNPHYITWAE
                     VINEKPDFHRPEFFGRLIFD"
     gene            complement(7723..9015)
                     /locus_tag="PK288_04950"
     CDS             complement(7723..9015)
                     /locus_tag="PK288_04950"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18301.1"
                     /translation="MQSINISRRKFVKGLAGSAAGIAVMGGNPGMSAMSYSRIIGANE
                     RITIGVIGCGGMANSHMEALLNMKQSDNVEITAVCDIYTNRLEKAKELTKAQAFKNYQ
                     EILQKKDIDYVLIATPEHWHYQMTLDAISAGKHIYVEKPMTHTIEQAKDICNKISSSK
                     LKLQVGVQGMSDDSYETAHKMITEGALGKVVMAHIDYSRNYPGDFWAYEIDPDARPGV
                     NLDWEAWLGPAPKRPWDPRRYFQWRRYWDYSGGIATDLFIHRVTRIIKSVGLSFPDYV
                     VATGGTWNFVNSVAEIPDTFNMMLDYPEKITVMVVSSLANDMPIRHVIRGHKATLEFT
                     KEGFTITPQEQSELAKISGTGETVEGTNLFTYRKTGAEDVTLHHRNLIAAIRKSEPLK
                     CDSNLGLYGVVACMMGVESFRNRKYLKWDQQNKMIIES"
     gene            complement(9135..10589)
                     /locus_tag="PK288_04955"
     CDS             complement(9135..10589)
                     /locus_tag="PK288_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18302.1"
                     /translation="MIKSGRRKFITRSLLGTAGVALRGLIPLDTRDLPGADERGSLKP
                     GPATSLKVQTEEMVYRYVPADNGAGPMWCHGSTCIVRAGNQVYATGIETVPGWKPLNN
                     CRWTLFRRSAAGWEHVMTDEKDRTREPSPVAVLNNGHIYLSVNPTLTMPEEYNGPSKP
                     GFYVFDSRDPQLKYQQINPVWDGEPPFTEHSYRSLAADGPSNELILFQNIGYTHAEWT
                     FLDRENRWASQGRIVWPWGSDYEVPGPIRVCYPTVMLKNRAVYFCGVSDIIEPRREWR
                     EYKRQLTGNEWDYDFRRLFFTWTDDVTQKPFHDWIEVASREDTCGWIFPSDLYAAGDG
                     SVYILWTERAIDERLREKFFPGRKQSHALKYALIRNGRVISENTLHIMEEGGTNGIPV
                     EARFQILPDQRIFVVYAIRENLTADQSAVKNYIMEIGPGSKTTEPVLIPLRYPFSSFF
                     TATVRGGSEPSSSLDFLGTTSEKNTIRYARVILD"
     gene            complement(10642..12606)
                     /locus_tag="PK288_04960"
     CDS             complement(10642..12606)
                     /locus_tag="PK288_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPM18303.1"
                     /translation="MKLTAFIFLILFSTGNLLSQVFTIGSVYDLDPVFEDGYKLPAMK
                     DTLKIFGIRGEVLSGQFVINAKKQLNDVSVHLGDFREMSSGSLLPQEAIEWNFVGNIP
                     LKKNTPNQPERILVRKAPARFPDYLMQERSVQVPQKTYRAVWLTFSIPEGAREGTYSG
                     KATVKCSQGEQSLPVLVKIYPLTLSSGRHLKVTEWYSTDHFRRFHGITEKYTPEWFAM
                     LRRYAENMVSHRQNIFHVPMSAIEISGTGEGRFRFDFTKFDQTANVFWETGKMDYLET
                     GDLFRFGEDAWFSTNLKFKDYRIRNERTGTVETVKGEEIVPLLLPEFESHLRQKGWLH
                     KTLFHIQDEPSMHNSLAWREKSSYVRSLAPDLKRIDAIETTNVLDEIEIAVPKIDALA
                     TWHRSYEDARRNGIELWFYTVGIYQGSLFPNKTIDLPFMNSRLLHWLNYKYDATGFLH
                     WGWNQWEEDPFSDTGMHIGDGWHVYPAKDGVLNSLRWEQMRNGIQDYECFKMLEDKIR
                     CLRDSLGSRASWIDPSQRGKEIASKIVKDFDEHSNDPRSFYNAKEELIREIISFDNSP
                     GIYIQTNPVDGSSITLHSSVELFGWTIPGTKIVVNGKELPVSDQGYFFEQFGGEELDN
                     TKIGLSESSVFVQASKDGKTTEIVRNFKVY"
     gene            complement(12611..15511)
                     /locus_tag="PK288_04965"
     CDS             complement(12611..15511)
                     /locus_tag="PK288_04965"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HPM18304.1"
                     /translation="MKNKLFISSILLFLAAMIQITAWAQVNEPVMYIPSAGSIPVADG
                     KITEPVWQQAAELTEFINWSRDSYIKDSVKVLVCSDAKNLYVAFRNSDPGAARLMKSV
                     SPKGPRDTFLWGRNHVSVGLSYGGKSIRLMADPKGTMTDWKGDDLSWNGNWQYGASIN
                     SHDWSAEFIIPLSDLSESGIAPGEEIKISISRSFPMGESSEWSGRCVLSAKENLKCTF
                     GRWPEPVPGKNFLPFSASNFGNAEADIKCVIKLYKLDKKPVFINQTGQGASSEMQLVI
                     SSEPLIYNYEYSLPARQSVEQKLQFDLPYEGSYFASASVLSAQGSIIRRGVDFWFTIE
                     PNRQKLNSLKGLIGESIASLTRLSGPVVDDLKKKAESYYSDVLKLESSAENAWSSARW
                     NELTDKTDKLKSDISRHLNCLRWNTLMNWNDTWDFGISLTHSVVKLKKDEYFPFPVTD
                     RINISLAGNEYESFQLALLPFSGDMKNMHVRVSDLKSKDGSLIGNKNIEVSLVDYNMI
                     EWQADYVAEKGWHPDPLIPFRNGMTIDGSDLCRPLWITVYAPAGTKPGNYQGTIEISA
                     DGMKKVIAFVECRVWGFDLPVTSHLKTHSWDNIEYMADFYNLEEYPVEWYMNFCQLLL
                     KNRFNPGSAGINYVSEKPVRKGKYDFSRVEKVLQFCIDRGLSRFSMIQMRKGLYTPKE
                     EAEVYKFIEAYSRFLKERGWLDEALVELWDEPTDLEWPYIRERAEKIKKIDKDIRLQL
                     FAEGGPYEFSDRSTDKYGLNDLVNIWAPVNIIESPETQAKGGEIWTYFCTLARESAPN
                     FFIDCPAIYQRSIPWYCWMYGVDGFEHWSTNYFWRNVKRGEPVDNKWPNTPWDSRTYY
                     YYNGEGQLVYPGPEGATYSSIRLENFRDGMEDYEYLFRLRELLSAAGNEIDDPMLNSY
                     RQLLKPEDYLLIKNPRKIKVTLENTLRFPDQPELILERRNMIANAIEKLLKK"
     gene            complement(15554..17356)
                     /locus_tag="PK288_04970"
     CDS             complement(15554..17356)
                     /locus_tag="PK288_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPM18305.1"
                     /translation="MKASSTFKDSTKGRFGFYAFCSGLAFLVFLLSGLTLFAQSPDDN
                     GNVKVWAVPGEQKVRPDDKPETANLVWSAADKQVSIAGAGNEHVPFQVVISTPVPPGW
                     RPKAPDGFFVKASGLRSDRGDLIGKENVKLYLEHYIQIYAVSSPVGATGYWPDALAPL
                     SEPFSMQAQYIVVGNRPVWIDVFIPSGTPKGIYTGTVTVERFGKVLETIGVSLEVYGF
                     SLPDETHIITYMNISKNEVTRFYNASGSAPVTEELMRNYFSFLFDHRMETWFNDPLVP
                     EITLKGEKVDLKFNEERYDYYMNKLKLKRVILETVPHEITRQLKTEPFSPVFNRVVKS
                     YLTSVESFFRKRGWLDRLVFNSPIDEPNTKKDYEETRLYAQLVHEAVPGVPFLSTESP
                     VSDNPEWGTLRGYVNNFCVHGNALNDPEVKQALLEEQAKGGEATWYISCDQAYPQPNY
                     FIDAPALDPLMVPWITERYKMNGFLYWAANFWNQTPDPWLDPVTFISGFECSGGYVLN
                     GEGSLIYPGNHTRRYTGQPDVNGPVSSIRFELLREGIEDYEYIWLLKEKGDPAFADEI
                     VRNMVIDVSTFSRNLAELYLSRKAMAKRLEELVK"
     gene            complement(17550..21143)
                     /locus_tag="PK288_04975"
     CDS             complement(17550..21143)
                     /locus_tag="PK288_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPM18306.1"
                     /translation="MIKPSGRFFFFFIIFFFVIFSSCQNERFKKRASSKTLFRLLDPK
                     ECGITFANRLTESDTDNAIFYEYYYNGAGLAVGDLDNDGLSDIVFGANMTECRVYLNK
                     GGLKFADVTRQCGVNTAGKWITGVSLADVNNDGWLDIYLCAAGNIDYDYTNMLYISNG
                     DPDNLFFTECAAAVGLADRGYSTQAVFFDYDRDGDLDMYLVTASMTIPNKNALRQRKN
                     DGSMPNTDRLYRNEGINPLTNLPFFRNVSREAGITWDGFGLGVAVCDINKDGWPDIYV
                     GNDYISNDLLYVNQKDGTFREMVQDYTKHITLSTMGLDIADFNNDGFVDLVLLDMQPE
                     NYYRKRTMALNMRDYKRYLSELNAGYSPQYIRNMIQVNNGNINGRITFSDIGQLAGIF
                     ETDWSWGTLFADFDNDGFKDLIIGNGIPHDVTNMDVSELWMKTIRANREIDFTVLYKL
                     LKAEMDKLGNVKKPNMIFRNNGSLIFETKTREWGLGRPSYSTGMVFADLDNDGDLDLI
                     MNNINDPAFVYENTLMSVDSLKSRNHFISLILRGDTKNTGGIGTKVTVYHDGRLQYHE
                     HFPVRGFQSMVDSKIHFGVGTSESIDSIIVCWPDEKVQKLRNVPSDQFIEVRYIDAKP
                     DRIFENKTGNPSLFIQVPEKNGIKYIHRERQFIDFDIQPLVPHQYSKEGPGIAVGDIN
                     SDGLDDFFIGGSTSYSGMVFIQGNNSVFTSYPLPGENNYEDMGALLFDADGDGDNDLY
                     VVSGGAGLPPGIEFYNDRLYINNGKGGFIMDRNALPDERVCGSHVAAADFDRDGDLDL
                     FVCGRVSLENYPLPPRSFLLRNDSKGSQIRFTDVTGALAPELERPGLLASALWTDFNN
                     DGWPDLIIAGEWMPVMFFVNVQGKLRNITGSTGLDKYTGWWNSIAAGDFDRDGDMDYA
                     VGNLGLNTQYKVSPAEPMRIMARDFDLNGTIDPVCSYYVQGKSYPIYHRNVLLSQIPS
                     LRNRYNTYEAYARATLDDIFPEDTRKGAYIKECTWFASSWIENLGNGAFRMHSLPEEA
                     QLSPVFGMLAGDYNNDGNCDLILTGNSYSSNVYTGQYDASIGLFLSGDGKGGFKPVLG
                     RESGFFVDGDAKSLAEISLKDGNSFILAARNSDTLRVFKPLAKPAGIIRLGKDDFCAE
                     IILKNGTREYREFYYGSGYLSQSSRVLKLPPEAASVTVMTFTGEKRIITMK"
     gene            complement(21260..>21615)
                     /locus_tag="PK288_04980"
     CDS             complement(21260..>21615)
                     /locus_tag="PK288_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM18307.1"
                     /translation="LFAEAENQVNGPTQAACDAVNQIVNRATGGVPNPDDPLFTTSMT
                     KEEFDEAIIQQRSWELCFEYDRWFDIQRKRILDKVTDPEFLQNYSIEDYLLPIPQKDL
                     RLNKALVQNPGYTSP"
BASE COUNT         5839 a         5149 c         4530 g         6097 t
ORIGIN      
        1 aatgcaaagg catccgtgct atgagcagaa aaatttgcta tgagcggaaa acggcaccct
       61 gatgtcatgc atgcgcagaa gtgaaaagaa ttttttaaag tcgaccattc cctggcccag
      121 aggcaccggt tccgatatag tcttcccgtt tttttcaacc cacatgaagt cttttatgtc
      181 aatggtgcgg acccggggac tgatcagcct gagtccgacg ggccaggcat tcgctccttc
      241 aacagttgca tggtaaacat catactgtga gccaagccag ggagagttaa ttttttcaag
      301 gatcgcagca agatcccaga ttggcgcacc aaaatatatt ccctgtccat agtcacctga
      361 atgattctga tactctcctg aaatagagta ttttgcattc agatttgcca gcttttccag
      421 ctgttcccgg atcctggcca gatcgccgct tacagcattc cggtcattgt aatgaaaata
      481 acctgttctg taatgcttta ttcccaggga tgaagctgtt ttcaatattt tctccgaaag
      541 cgggtcagca gcatcggtca cagaagtggt tatcataatg acccttttcc cttttttggc
      601 aagtgcctcc accgccctgg gcagatccgc ctccacgttc tcgggaagaa catgccctcc
      661 cggacgcact gtgagatctg ccccgtcgaa tcccaggtcg gacagaaccc cggccatgcc
      721 attgtaatcg agccactgga gatgcttcga aaagatactg atcctgaaag catcaggatc
      781 gggggatgac aatgatccaa tgttgaaatt cgaatcctca gtcttatgcc cgtcaatagc
      841 tttaatatca aagcctgcag agaatcctgc tgctgcaaac agggattttg caataaaact
      901 tcttctcgta gttcccattt cttctttttt aattgaaatt gatcctgaaa agataataag
      961 atccggaaaa ggaacctata ttatcccggg aaattttccc gaaggctgta atgataattt
     1021 catctatgcc gttcaatgtt tacccctgtt tatgaaccgg gccgggattt gctacaaatg
     1081 gcaggaaatg acccggacct gtcttcaacc agaaacagcg tcctgctgga ggattaaaac
     1141 cggtatttta aaattgggta ataaatcaat tattactatt tttcggatta aggagtctct
     1201 gaggtgatca aattgaattt atcagttatt ggatcggtta tctttgcaac tgcaacattc
     1261 atagccggtt gcaatggaat tgacattcgg gatattcaac ctgcaaacag acagccggct
     1321 atagaacccg attactccgg gataaccatt ccatacaaca tcgctcccat gaatttcagg
     1381 atcggggaag agggaaagaa attccttgta aggatctctt cttcaaatgg aatctgcaat
     1441 aaaatcagat cgtcggacgg aaatgtgatt tttccattaa aactctggcg ggcactgaca
     1501 gggaataatg cagacagtat tgagattgag attattgctg taaacaagga taaaaagtat
     1561 ataaaatatt ctcctttcag tatgaaaata tcgggtgatt ctgttgaccc ctggctctgc
     1621 taccggtttc tttatcccgg ttatgaaacc tgggttgaaa tgcaactgat actaagatca
     1681 acagaaacct tcaaggagaa atatttcatt gagaaccagc tgctggaaag gaactgcatc
     1741 aactgccatt ctttcaatag caaaaaagct gagagattcc tggtgcaggt caggggatca
     1801 aagggaggca cttatttcca tgacggcatg gatctcacaa gaagaatcct ccgaacggac
     1861 aatatgaagg caaatgtcgt ttaccccagc tggcatccct caggaaaata cgtggctttt
     1921 tcatcaaaca ggaccataca atcgtttcac atgaaagcgg gcaagaacat tgaagtaatg
     1981 gatctgttct cctccctttt actctatgat ccggatcaaa atttaatctc cccggtcagt
     2041 gacagggata ctgcgaacta catggaaact tatccgtgct gggcgccgga tggaaaatac
     2101 ctgtactatt gcagggcaga gcaggtaatg gaaggctttg attacagaaa aataaagtac
     2161 gaccttgccc ggaaacgatt cgatagtgat accggggaat tcggtccggc cgaagttatt
     2221 ttcagtgcat ctgaaataaa caagtcagtg tcgtttccca gcatctcgcc cgatggcaga
     2281 tatctggttc tcacccttca cgattatgga acattctcaa tctggcaccg ggaagccgat
     2341 ctgtatcttc tggatctgca aaacatggat atcaaaaagc tcccgtttaa cagcaatgaa
     2401 accgaaagtt atcattcatg gtcatcaaat gggagatgga tcgtgttcag cagcaagcgg
     2461 ctcgacgggc tcaccgcaag gccgtttata gctttttttg aatctccagg caaaacagga
     2521 aagccatttg tccttcccca gaaggatccg actctttaca gacgactgga taaaacattc
     2581 aaccgtccgg aatttgtgcc tgcactgccg gcatcggaag cccgtgattt tatgaaagca
     2641 tccgctaaac cgcctttaaa agccagatgg atagaaaaat gaccagaaaa aatctgcccg
     2701 tcctgatcct tttcatcaca tcgtttgcat acctctactg gttcgcagcc ggaatttttt
     2761 tctaccagga acaaaaatcc ctttttattt tctcgtacga atatcttgcg aaacatcttg
     2821 tgaaaccggg aggattattg aaatatctgg caggtttcct aacacaggga tatttgtcag
     2881 atctgtacgg ggcctttatt aactcactga taattgcact actggcaata ttgttaagat
     2941 cagtggccga aaggctgtca ggcagcagat cattcacaat gccattagcc tttctgatcc
     3001 ctctcggatt gatgatctgt cagagcaatt atcatttcaa catactgcat tcacttggct
     3061 tcctgctggc agcagttctg ttcctgatct ccctgaatct taccaggaaa acctttcatg
     3121 tgatcctgat cttccttttc ccggtatttt atttcatgat gggaacctac tccctgatct
     3181 atctttgcac atatctaatc cacagtatct tatatcaaaa caacagatat ggaagaattt
     3241 ttaccctgct ccagatattc atctccattg tcacgctggt gctattctat aaattgttcc
     3301 tttatcagcc ttttagtgta atcacaggtt atcctttcat ctcaaacgaa tacggaaggg
     3361 caaacggact gctttacggt gttacagtat tgtttgtact tctccccggc cttattaaaa
     3421 tttcagatct tcccggatct gagaaacatg caactgcaaa atcaacggtt attgcagcat
     3481 gcctctatgg aatatcaata attgttcttg tccgccagta tgatccggta tttgagggga
     3541 tcatgaggac ggaaaaaaga tactgcagcg gaaaatttga tgaaatcatt gtgcatcacg
     3601 aaaagtatcc ctcatcgaat ataattgaac agttttacta taatctctca ttatcagaaa
     3661 aaggaaagct ttgcgacagg atgttcttcg gctgccagga taccggcccg atgtcacttt
     3721 cacttggagg taacagggaa caggcatcca gaacaatgca ttactactat gccgttggtc
     3781 ttgttaacga agccaggcat ctggctttcg agctgatggt cattaacggt tattcacccg
     3841 aaaatctcaa aatgctgata aaaacggaac tgataaacgg caatttcagg gtggctgaaa
     3901 gatatctcaa tattcttaaa aagaccctgg gctacagaac tgaagcagga aggctgggga
     3961 aaatgcttga aaatacggaa cttgtcaata ccgatgatga actgggggag aaaaggaaac
     4021 tgatgccgaa agatgatttt tttatccaca ctgaggatgc aatgaatatt gacctgctgc
     4081 tcaaatcaaa tcccgcgaac aggaaagcat tcgaatacaa gatggcaaga ttattgctgg
     4141 agaaagacat cctggcaatt gcagaagaat ccgtaaagat ggctgacatg ggttacacaa
     4201 ctattccacg gcatattgaa gaagccattg tggctttcgc cagttataaa aaagctttgc
     4261 cggatcttgc cggactggtt ttgtcagcag gcacgaatgc aaggttttta aattatgtac
     4321 gatccgttaa cagtttcgcc ggagacaggg ctgcaatcaa aaagtccctt aacagggcgg
     4381 aaaaggatac attctggtat tatcttcaat ttggacaaat cagaggagat tttacaagaa
     4441 gcagacctgt tgacaggaat gtgtactgac aacatctttt acggttttta ttccggaaat
     4501 taattcccgg taatactttt gtaataaatt caataaaaaa agcttgattt atacccggat
     4561 atgcatgaat tactcaaaat agccatcaaa atgagaaaaa caacctttct gtttttcctg
     4621 tttgcagcgg tcagtctgtc actatccgca caggaagaca aacttattac ggtaaaggca
     4681 ggccataaaa tactggatta ctttcccgat gaaataagat acttttaccc tgaattcaga
     4741 accggaagag tggttttcag gaacggcatt tattccgaaa ggctgctgaa ctataatttc
     4801 ctttccgggg aaatggaatt tctccagtcg cgcgataccc ttgcaataat aaacaagaaa
     4861 gatatcaggt atgttggaat tgaccgggat acgttttatt acgataaagg ttatataaag
     4921 caatgggcca gaggggaaaa ggtgattgga atgaaggaaa ggtttgagtt aaaggagatc
     4981 cagagcaggg attcctacgg cacctcaagt gccggtgctt cgacaaatac atacggttca
     5041 atgccggcgg atggcaattt ctacaagctc agcgcaaaca aggacatggt ttttcaacgc
     5101 accaggcaat tctacattgc tgaccccgac gggccctttg taattttaaa caggaaaagc
     5161 ctgctggctg cctatcacgg cagggaagac aagattaaac agtttctgaa aaccagccgg
     5221 ataaaattca attcccctcc ggatatctcg aaactggttg attttcttaa taaacttgat
     5281 taacaactgg tcaaaatgca catagaaaca aatcgtctgg tatttccctt aatattggcc
     5341 gggatcttcc tcacaggctg cgtacaaaaa aagaatcagc agaaagtcga attaaaaatg
     5401 cttcaggatg agaaatggtg gggaggagca gttacggatg gcaggaaatc accctatgct
     5461 gaaaggaatt tttattatga tcaactgggc gactgcaggg gaaatcaggc tcagccacta
     5521 tttctctcaa acaagggaag atacatatgg agtgagaaac ctattgagat atccatcagc
     5581 ggtaatataa tatcagcagg ggcccatgga gctgaaatca gtaccggcac ttcggggaaa
     5641 acactgaagg atgcattctt atattgcagc agaacgtttt ttccgccttc agggtcaacc
     5701 cctgatcctc tgatgttcac aaatcctcaa tataatacat ggattgaact acagtatgac
     5761 cagaaccaaa cggatgtgat cagatatgca gaggacataa tcaaaaacgg tttcccgcca
     5821 ggagttctga tgattgacga caactggcag gagagatacg gaacctggga atttgactgc
     5881 aggaaatttc cagatcctgc atcaatgata gacaagctcc acaggctgga cttcagggta
     5941 atgctctggg tttgtccttt catcagttct gattcccctg tgtaccgtga tcttcagggg
     6001 aagaagctta tggttttcag ggataaggaa aaaacagctc ctgcaattgt ggattggtgg
     6061 aacggtgcca gtgccataat tgacctgtca aatcctgaag gagcagcctg gttcagggaa
     6121 cagcttgata acctggcgga gaaatatggc gttgatggtt tcaaactgga cgccggggat
     6181 cctgaatact atacaggaac ttattctttc ggtgattcta gcccaaatga gcattgtgaa
     6241 tcatttgcaa aaattggtct cggatatccg tttaacgagt acagagcatg ctggaaaatg
     6301 gccggacagc ctcttgccca acggctgcgt gacaaaagtc acacatgggc tgatctccag
     6361 gtgcttattc cggatatgat cggacttggg ctgacaggct atcagtttgg atgccccgac
     6421 cttatcggag gaggtgagtg gacatctttc caagattcca gcatactgga tgaagaattg
     6481 attgtgagat cgtcgcagat tcatgcgctg atgccgatga tgcagttttc agtggcgcca
     6541 tggagggtcc tcagtaaaga caacctgaaa atatgcaggg aaatggcaaa tctgcatctt
     6601 tcgatgggag gtgagatata tgaacttgca aaagagtgtg ccgcttcagg ggaacctatg
     6661 atacgcaacc tggagtatga gtttcccggt aaaggttatg aaaatgtcaa agaccagttc
     6721 atgctcggga aaaatattct ggtgaccccc gtggtcttaa agggacaaag gtcaagggaa
     6781 atcatagtac cggaaggccg atggcaggga gacgacggaa ctacggttac aggaccggta
     6841 aaaattatca ttgacgtgcc cctggaaagg cttccatggt accggaaaag atcctgatca
     6901 gtcaaatatc agccgtccga aaaactcggg cctgtgaaaa tcgggctttt catttattac
     6961 ctcggcccat gtaatataat gcgggtttga ggtgatctcg gcaatcttgt agaaattagc
     7021 cttccactcc actccctttt gcggacgagc tgcattggaa tacttttcaa gaacatccag
     7081 cgggatcctg tattccagtg tccattcaac aggattgctg atttcaggat caataatctt
     7141 cgggagggta gaggctattt cgaccttttt aatgtcatcc agatcaatcc tctttgattc
     7201 cttccttggc accaggttat atgacaggag cggagtacct ccgcagtttg tctcgaggtt
     7261 gaaatataaa agaggcagcc ggggatcggg tgcaaagaag aattcaaccg cactgtcctg
     7321 ccataccggc ccgttaatcc ggtcggtaat acattttaca taacgatcct ttacgtgaaa
     7381 gatcacataa acataatcat tgtcatacaa cattttcacc tgcgtttcag gtttaaattc
     7441 cggaaccgtg cccatgtaat ttgtaatatt gattgatctt acatttttcc actgggcctt
     7501 gttccagtca gcgtctattt tcatcggctt tttcagcttt ccgacatgat aaaccggcgc
     7561 gtcatccgta accggagctt ctgaaaatga acaaaaagta ccaactaaaa tcattaaacc
     7621 agtttttacc aaaatattct tcatcttcat ctttattaat cttcgttaaa attaaaatca
     7681 gtttccgggt taatcagggt aatatcaatc acacttgaat aatcagcttt caatgatcat
     7741 cttattctgc tggtcccatt tcagatattt tctgttacga aatgactcga ctcccatcat
     7801 gcaagctaca acgccatata aaccgagatt cgaatcgcac tttaatggtt cgcttttcct
     7861 gatcgcggca atcaggtttc tatggtgtaa agttacatct tcggcacctg ttttcctata
     7921 ggtaaaaaga tttgtccctt ctactgtttc tccggtaccg cttattttag ccaattctga
     7981 ttgctcctga ggagttattg taaatccttc ctttgtaaat tccagtgttg ccttgtgccc
     8041 tcttataaca tgccgtatcg gcatatcatt tgccaatgat gaaacaacca taacagttat
     8101 tttttcagga taatccaaca tcatgttaaa agtatcgggt atctcagcca cactgtttac
     8161 aaaattccac gtcccgccgg tggcaacaac ataatccgga aagctaaggc caaccgattt
     8221 aatgatcctt gtcacccggt gaataaacag atctgttgca attccaccgg agtaatccca
     8281 gtatcttctc cactgaaaat atcttctcgg atcccagggg cgtttgggcg caggaccgag
     8341 ccaggcttcc caatcgagat ttactcctgg cctggcatca ggatcgattt cgtacgccca
     8401 gaaatcaccg ggataattcc ttgaataatc aatatgtgcc atgacaacct tcccaagcgc
     8461 tccttccgta atcattttat gtgcagtttc ataagaatca tccgacatgc cctgtacccc
     8521 gacctgcaat ttgagttttg acgatgaaat tttattacat atatctttag cctgttcaat
     8581 tgtgtgggtc attggctttt cgacataaat atgctttccg gcgcttatgg catccagagt
     8641 catctgataa tgccagtgtt caggtgtggc tataagtaca taatctatgt cctttttctg
     8701 caggatttcc tgataattct taaaagcctg agcttttgtc agctctttag ctttctccag
     8761 cctattcgta taaatatcac atacggcagt aatttcaaca ttgtctgatt gtttcatatt
     8821 taacagagct tccatgtgac tgtttgccat gccaccgcaa ccgatcacac cgatagtgat
     8881 cctttcattc gctccgataa tacgcgaata actcatagct gacatccccg gattgcctcc
     8941 cattactgca attcctgcag cacttcctgc caatcctttg acaaatttcc ttcttgaaat
     9001 attaatactt tgcatttttc gattgtctaa tctggttagt aaactttgca tgacaactgt
     9061 tgtggatcta agcccatttt aacaagatac agtttttcat tttaacctgt gggaaattca
     9121 aaaaataaat taggtcaatc cagaatcact cttgcataac gtatcgtatt cttttcactg
     9181 gttgtgccaa gaaaatccag ggaagaagaa ggctctgacc cgccacgcac tgtagctgta
     9241 aaaaaacttg aaaagggata cctgagcggg atcagtaccg gttcagtggt ttttgatccc
     9301 ggacctattt ccatgatata gtttttaact gctgactgat ctgcagttaa attctccctt
     9361 attgcataca ccacaaatat cctctggtca ggcagtatct gaaatcgcgc ctctaccggt
     9421 ataccgtttg tccctccttc ttccatgatg tgaagggtgt tctctgaaat gaccctgcca
     9481 ttcctgatca atgcatattt aagggcgtgc gactgcttcc ggccggggaa gaacttttcg
     9541 cgcaatcttt catcaatggc tctctccgtc cataggatat aaaccgaacc gtcgccggct
     9601 gcataaagat ccgaagggaa tatccagccg caggtatctt cacggcttgc cacctcaatc
     9661 cagtcatgaa aaggtttttg ggttacatcg tcggtccagg tgaaaaataa cctcctgaaa
     9721 tcataatccc attcattgcc tgtcagttgt ctcttatact ccctccattc ccttctgggt
     9781 tcaatgatgt cactgacccc gcagaaataa acggccctgt ttttcagcat tactgtcgga
     9841 tagcataccc ttattggccc gggaacctcg tagtctgatc cccatggcca gacgatcctt
     9901 ccctgtgacg cccaccggtt ctccctgtca agaaatgtcc attcagcatg agtgtaaccg
     9961 atattctgga aaaggataag ctcattcgat ggaccgtcag ctgcaaggct tctgtaggaa
    10021 tgttccgtga atggcggttc accgtcccat acgggattga tctgctggta ttttaattgc
    10081 ggatcgcggg aatcaaaaac atagaatccg ggttttgagg gaccattgta ttcttcaggc
    10141 attgtgagag ttggattgac agaaagataa atatgcccgt tattcagaac agcaaccggg
    10201 ctcggttccc tggtccggtc tttctcatct gtcattacat gctcccatcc ggcagccgag
    10261 cgtctgaaaa gcgtccagcg gcaattgttg agaggcttcc agcccggaac tgtttcaatc
    10321 cccgtcgcgt aaacctgatt ccctgcccgt actatgcagg ttgaaccatg acaccacatc
    10381 ggacctgcac cattatcagc gggaacatac ctgtaaacca tttcttcagt ctgcaccttc
    10441 agggatgttg ccggccccgg tttcagggat ccccgctcat ccgcaccggg taaatcccgt
    10501 gtgtccaaag gtatcagccc tcttaaagcg acgcctgcag ttcccagaag gctccgggtg
    10561 atgaattttc tcctgcctga cttaatcatt cctttttata atattatgaa ttaataaatc
    10621 accgactcag ttatcactgt ttcaatatac cttgaagttt ctcactattt cggttgtttt
    10681 accgtctttt gaggcctgta caaaaaccga gctctcggat agtcctattt ttgtattgtc
    10741 aagttcctct cctccaaact gttcgaagaa ataaccctga tcgcttacag gcagttcctt
    10801 gccgttcaca actattttcg ttccgggtat tgtccatcca aaaagctcaa ctgaagaatg
    10861 taatgtgata gatgaaccat caacagggtt ggtctgaata tatatcccgg gagaattgtc
    10921 aaaactgatt atctccctga taagttcctc ctttgcattg taaaacgacc ggggatcgtt
    10981 ggaatgttca tcaaagtcct taacaatttt cgaggctatt tctttgcccc gctgcgatgg
    11041 atctatccag gatgcccttg atccgagtga atccctgagg caccttattt tgtcctccag
    11101 cattttaaaa cattcataat cctggatccc gtttctcatc tgttcccacc tgagagaatt
    11161 caagactccg tcttttgcag ggtaaacatg ccagccatcc cctatgtgca tacccgtgtc
    11221 actgaatgga tcctcctccc actggttcca tccccagtgc aggaagccgg ttgcatcata
    11281 cttataattg agccagtgca gcagccggct gttcataaac ggcagatcaa tagtcttgtt
    11341 gggaaacagg cttccctggt aaatccccac tgtgtagaac cataattcga tcccgttcct
    11401 gcgggcgtcc tcatacgatc tgtgccatgt cgcaagtgca tcaatcttgg gaacagcaat
    11461 ttctatttca tccagcacat ttgttgtttc tattgcatca atccttttaa ggtcgggtgc
    11521 aaggctgcgg acatatgaag atttctccct ccaggcaagg gaattatgca ttgacggttc
    11581 atcctggata tgaaaaagtg ttttgtgcaa ccagcctttc tgtctcagat gactttcaaa
    11641 ttcgggaaga agcagtggaa caatctcctc tcccttcact gtctcaaccg ttcccgttct
    11701 ttcattccta atcctgtaat ctttgaattt caggtttgta ctgaaccagg catcttcgcc
    11761 aaatctaaaa agatcacctg tttcaaggta atccatcttt cctgtttccc agaaaacatt
    11821 ggcggtctga tcaaattttg taaagtcaaa tctgaacctg ccctcccctg ttccggatat
    11881 ttctattgcg ctcatcggta catggaaaat attctgcctg tgtgaaacca tgttttcagc
    11941 atacctgcga agcattgcaa accattccgg cgtatatttt tcagtgatgc cgtgaaaacg
    12001 ccggaaatgg tccgtggaat accattcggt aactttaaga tgcctcccgg aggaaagggt
    12061 aagtggataa atcttcacga gaacaggcag cgactgctca ccctgactgc attttacagt
    12121 tgctttccct gaatatgttc cttccctggc gccttcagga atggaaaaag taagccagac
    12181 tgccctatag gttttctggg gcacctgcac actcctttcc tgcatcaggt aatcgggaaa
    12241 ccgggcagga gcttttctga ccaatattct ttcaggctgg ttgggagtgt tttttttcag
    12301 gggaatattt ccaacgaaat tccattcaat ggcctcctgc ggcagcaggc tccccgatga
    12361 catctccctg aaatcaccaa ggtgtacact cacatcattt aattgctttt ttgcattaat
    12421 cacgaactgc cctgacagaa cttctcccct gattccaaaa atctttaaag tgtccttcat
    12481 ggcaggcagt ttgtacccgt cttcaaaaac aggatcaaga tcgtatacgg atccaatggt
    12541 aaatacctgc gacagcaggt taccggtgct aaacagaatg agaaagataa atgcagtcag
    12601 cttcatggtt ttatttttta agcagttttt caattgcatt ggctatcata tttctgcgtt
    12661 caaggatcaa ttcaggctga tccggaaagc gcagggtgtt ttcaagggta acctttatct
    12721 tccggggatt ttttatcaga agataatctt caggtttcag cagttgcctg taactattga
    12781 gcataggatc atcaatttca tttccggcag cagaaaggag ttccctcagc ctgaacaggt
    12841 attcataatc ttccattccg tcgcggaagt tctccagcct gattgatgaa taagttgctc
    12901 cttcgggtcc tggataaaca agctggcctt ctccgttgta gtaatagtat gtccgtgaat
    12961 cccacggcgt atttggccat ttattgtcga caggctcgcc tcttttgaca tttctccaga
    13021 aatagttcgt actccagtgc tcgaaaccat caacaccata catccagcaa taccatggaa
    13081 tgcttctttg gtaaattgca gggcaatcaa taaagaaatt tggagcggat tccctcgcaa
    13141 gagtgcagaa ataggtccat atctcacctc cctttgcctg agtctcgggc gattcaatta
    13201 tatttacagg agcccagata tttacaaggt cgttgagccc atatttatcg gtgctcctgt
    13261 ctgagaactc atatggtccg ccttctgcaa aaagttgcag ccggatatcc ttgtctatct
    13321 tttttatctt ttcagccctt tccctgatat atggccattc aaggtcagtt ggttcatccc
    13381 agagctcaac cagcgcttcg tcaagccatc ccctttcttt caggaacctg ctgtaggctt
    13441 cgataaattt ataaacttcc gcttcctctt ttggagtata taatcccttc ctcatctgga
    13501 tcatcgagaa tctggaaagc ccccggtcaa tacaaaactg aagcaccttc tcgaccctgc
    13561 tgaaatcata ttttcctttc cttacaggct tttcgctgac ataattaatt cctgccgagc
    13621 ccggattgaa cctgttctta agcaggagct ggcagaagtt catataccat tcaaccggat
    13681 attcctcaag gttgtagaaa tcagccatat actcaatatt atcccaggag tgggttttaa
    13741 gatgacttgt aaccggaagg tcaaatcccc ataccctgca ttcgacgaac gcgatgactt
    13801 ttttcattcc gtcggcactg atctcaattg ttccctgata attaccaggt ttggtacctg
    13861 ccggagcata aacggttatc cataaaggcc tgcaaagatc tgacccatcg atggtcatcc
    13921 cgttcctgaa aggaatcagc gggtcgggat gccacccttt ttcagcaaca taatctgcct
    13981 gccattcgat catgttataa tcaacaaggg aaacttcaat attcttgttt ccgatcaggc
    14041 ttccgtcttt actctttaaa tcgcttactc gcacatgcat attcttcata tctccactga
    14101 aaggaagcag tgccagctga aaagattcat actcattgcc tgccagggag atattgatcc
    14161 tgtctgtaac cggaaaaggg aaatactcat ctttcttaag ttttacaaca gaatgcgtaa
    14221 gtgagatgcc aaaatcccat gtatcgttcc agttcatgag ggtgttccat cttaaacaat
    14281 taagatgacg cgaaatgtct gatttcagct tgtcagtctt atcagtcagt tcattccacc
    14341 ttgcggaact ccatgcattt tctgctgatg attcgagctt aagcacatct gaataataac
    14401 tttctgcttt cttcttcaga tcgtcgacta caggtccgga aagtctcgtc agtgaggcaa
    14461 tcgattcgcc gatcagtccc ttgagagaat ttaacttctg cctgttgggt tctattgtga
    14521 accagaaatc aacaccccgc ctgatgatgg agccctgagc cgacaaaacg gatgctgaag
    14581 caaagtatga tccttcataa ggcaggtcga actgaagctt ttgctcaaca gactgccgtg
    14641 caggcagcga atattcataa ttgtaaataa ggggttcaga gcttattaca agttgcattt
    14701 cggatgaagc accctgtccg gtctgattaa tgaaaacagg ttttttgtcg agcttgtaga
    14761 gttttattac gcatttaata tcagcttctg cattcccgaa atttgatgcg ctgaaaggca
    14821 ggaaattttt acccggaaca ggctcgggcc acctgccgaa agtacattta aggttttctt
    14881 ttgccgaaag cacgcatcgg cctgaccact cggacgactc gcccatcgga aagcttcttg
    14941 agatggaaat ttttatttct tcacccgggg caattcctga ttcagacaag tcactcaggg
    15001 gaataatgaa ttcagcagac caatcatgac tatttattga tgccccgtat tgccagttcc
    15061 cgttccagct cagatcatcg cccttccagt cagtcatggt acctttggga tcagccatca
    15121 gcctgatgct ctttcctcca tacgacaaac ccacgcttac atggtttctt ccccacagaa
    15181 atgtatccct cggccccttc ggactgacgc tcttcatcaa tcttgctgcc ccaggatcac
    15241 tgttacggaa agccacataa aggtttttgg catcactgca aacaagaact ttaacggaat
    15301 ccttaatgta actgtcacgc gaccagttaa taaattctgt cagctcagca gcctgttgcc
    15361 agactggctc agtaattttc ccgtcagcca caggtatgga tccggcagaa ggaatataca
    15421 taacaggttc attcacttgt gcccatgcag taatctgaat catcgctgcc aggaacaaaa
    15481 gaatgctgct aatgaataat ttattcttca ttactataaa acctttctag tgttaattga
    15541 ttaacagaag atcctatttt accaactctt ccaagcgttt tgccattgct ttccggctta
    15601 aatacagttc agcaagattc ctgctgaaag tacttacatc aataaccata ttccggacaa
    15661 tctcatccgc gaaggcagga tcgccttttt ccttcagcag ccatatatac tcgtaatcct
    15721 caattccctc cctgagaagc tcaaacctga ttgacgacac agggccgtta acatccggct
    15781 gcccggtata tcttcttgta tgattgcccg gataaattaa tgatccctcc ccgttcagaa
    15841 cataaccacc gctgcattca aaccctgata taaaggtgac cggatcgagc cacggatcag
    15901 gggtctggtt ccagaagttg gctgcccagt aaaggaaccc gttcattttg taccgttctg
    15961 ttatccatgg aaccatcagc ggatcaagtg caggagcatc aatgaaatag tttggctggg
    16021 gatatgcctg atcgcatgaa atataccatg tggcctcgcc tccctttgcc tgttcctcaa
    16081 gcaatgcctg cttaacctcc gggtcattca gggcattgcc gtgaacacag aaattgttga
    16141 catagccacg caatgtaccc cactccggat tatcactgac aggagattct gttgaaagaa
    16201 aaggtacgcc cggaacagct tcgtgtacaa gctgtgcgta cagccgggtc tcctcataat
    16261 ctttcttcgt atttggctca tcaatcggac tgttaaaaac gaggcgatcg agccatcccc
    16321 gtttcctgaa aaaagattca acggatgtaa gatagctttt taccaccctg ttgaaaacag
    16381 gagaaaaagg ttccgttttc agctgcctgg taatttcatg gggtacggtt tcaaggatga
    16441 cccttttcag cttaagctta ttcatgtaat aatcgtacct ctcttcattg aatttcagat
    16501 cgactttttc tcctttcagg gtaatttcgg gtacaagcgg atcattgaac caggtttcca
    16561 tcctgtgatc aaacaggaaa ctgaagtaat tccgcataag ttcttccgta accggggccg
    16621 aaccgcttgc attataaaac cgcgttactt catttttcga aatattcata taggttataa
    16681 tgtgagtctc atcaggcagt gagaatccgt acacttccag gctgactccg atcgtttcca
    16741 ggacctttcc aaatctctca accgtaactg ttcccgtata gattccctta ggagtacccg
    16801 aagggatgaa aacatctatc catacgggcc ggtttcccac gactatatat tgggcctgca
    16861 tactgaacgg ttcacttaac ggtgcaagag catcgggcca gtaccctgtt gccccgaccg
    16921 ggcttgaaac cgcgtaaatc tgaatataat gctcaaggta gagtttcaca ttttccttac
    16981 ctatcaggtc tcccctgtcg gatctcagtc cggaagcctt tacgaaaaaa ccgtcgggtg
    17041 cctttggcct ccagcccgga ggaacgggag ttgagatcac aacctggaac gggacatgtt
    17101 cgttgcctgc tccggcaatc gaaacttgtt tgtctgcagc ggaccacaca agatttgcag
    17161 tctcaggctt atcatccggc ctgactttct gctctccggg tacagcccaa accttcacat
    17221 tgccgttatc atcaggcgat tgggcaaaca gggttaagcc ggacaacaga aataccaaaa
    17281 atgcaagacc ggaacaaaag gcataaaatc cgaatcttcc ttttgttgaa tctttaaatg
    17341 tactgcttgc tttcataaat aaattgtttt aacagtcagg ttttgattaa atattattat
    17401 ttcttcctta agccatcctg attctgaagg caatatgtca ttgtacccga tatggcaggt
    17461 tgtatgccag gcaaagcgtc tgttcattta tctgtttttt ccgggccggg atccgcgatt
    17521 gtgttcttct tccctatctg cccggaatac tatttcatgg ttatgatcct tttttccccc
    17581 gtaaatgtca taacagttac ggaagcagct tccggcggaa gtttcagaac tcttgatgac
    17641 tgtgacaggt aaccgctgcc atagtaaaat tcacggtatt ccctggtacc atttttcaat
    17701 atgatctccg cacaaaaatc atccttgccg agcctgataa ttccggcggg ttttgccaga
    17761 ggtttaaaaa ccctcagggt gtctgagttc cgggcggcaa gtataaatga attgccatct
    17821 ttcagcgata tctcagcaag ggattttgca tcaccatcta cgaaaaagcc gctttccctc
    17881 cccaaaaccg gcttaaagcc gcctttcccg tctcctgaaa ggaataaacc tatcgaagca
    17941 tcatattgtc ccgtataaac atttgaagaa taagagttac ctgtcagtat gagatcgcag
    18001 ttgccatcgt tgttataatc gcctgccaac atcccgaaaa ccggtgaaag ctgcgcttct
    18061 tcgggaaggg aatgcattct gaaagcgcca tttcccaggt tttcaatcca tgatgatgca
    18121 aaccaggtgc actcctttat ataagcacct ttcctggtat cctcaggaaa aatatcatca
    18181 agagtagctc ttgcgtatgc ttcatatgta ttgtacctgt ttctcaacga cgggatctgg
    18241 gaaagcaaca cattcctgtg ataaattgga taactctttc cctgaacata gtaactgcat
    18301 acagggtcga tggtcccgtt caggtcaaaa tccctggcca tgatccgcat gggctcagcc
    18361 ggggacacct tatactgggt gttcaggccc agattaccca cggcataatc catgtccccg
    18421 tccctgtcaa aatcgcccgc agcaatgctg ttccaccagc cggtatattt gtccagtccg
    18481 gttgagccgg tgatatttct gagttttccc tgtacattca cgaaaaacat tacaggcatc
    18541 cattccccgg caattatcag atccggccat ccgtcattgt tgaaatcggt ccatagtgcc
    18601 gaggcaagta gtccgggtct ttcaagttct ggcgccagcg cgccggttac gtcagtgaac
    18661 ctgatctgcg aacctttgct gtcatttctt agcaggaaac tccttggggg cagcgggtaa
    18721 ttttcaaggc tgacccttcc gcagacaaaa agatccagat cgccgtccct gtcaaaatcg
    18781 gcagcggcaa cgtgcgagcc gcagacgcgt tcatccggga gagcgttcct gtccattatg
    18841 aagcctccct ttccgttgtt tatatacagc ctgtcgttgt agaattcaat gcccggcgga
    18901 agtccggcac cgccactgac aacatacagg tcgttatccc cgtcgccgtc agcatcaaac
    18961 agaagtgctc ccatatcttc ataattattt tcaccgggca ggggatatga tgtgaaaact
    19021 gaattattcc cctggataaa aaccattcct gaataactgg tggatcctcc tatgaaaaaa
    19081 tcatccaaac cgtctgaatt tatgtccccg acggcaatcc ccggtccttc ttttgaatac
    19141 tgatgcggaa cgagcggctg aatatcgaaa tcgatgaact gcctttcgcg atgaatatat
    19201 tttattccgt ttttttcagg cacctggatg aacaatgaag gattcccggt tttattttca
    19261 aatatcctgt ctggctttgc gtcaatgtac ctaacctcaa taaactgatc ggaaggcaca
    19321 ttacggagct tctgcacctt ttcatcgggc cagcagacta tgatcgaatc aatggattca
    19381 gatgtcccta caccgaaatg gatcttcgaa tccaccatcg actgaaatcc tcttaccggg
    19441 aaatgctcat ggtattgaag gcggccatca tgatagactg taacttttgt tccaatgccg
    19501 ccggtattct ttgtgtcgcc tctcagaatc aatgaaataa aatggttcct cgatttaagg
    19561 gaatcgacgc tcatcaaggt attttcatag acaaatgccg gatcattgat attattcatt
    19621 atcaggtcaa gatccccgtc attatccagg tcggcaaaca ccatgcccgt tgaataggaa
    19681 ggccggccaa gaccccattc ccgggttttc gtttcaaaaa tcaggctgcc gttgtttctg
    19741 aatatcatgt ttggcttctt aacgtttccc agtttgtcca tttctgcctt aagaagctta
    19801 taaagaacgg tgaaatcaat ttcgcggttg gcccttattg ttttcatcca caattccgaa
    19861 acatccatat ttgtgacgtc atggggaata ccattgccaa taatcagatc cttgaagccg
    19921 tcgttgtcga aatcggcaaa taaggttccc cagctccagt ccgtctcaaa tattcctgca
    19981 agctggccta tatcactgaa agttatcctt ccgttgatat tgccattatt cacctgaatc
    20041 atattcctga tgtactgggg tgaataccct gcattcaatt cgctgagata tcttttgtaa
    20101 tcacgcatat tcagggccat ggttcttttc ctgtagtaat tttcaggctg catgtcaagt
    20161 aaaacgaggt ccacgaaccc gtcattattg aaatctgcaa tatcaagacc catggtgctc
    20221 agggtaatat gcttcgtata atcctggacc atttccctga aagtgccgtc tttctgattc
    20281 acatagagca ggtcattcga tatgtaatcg ttgcccacat aaatatcggg ccagccatcc
    20341 ttgttaatat cacaaacagc gactcccagg ccgaatccgt cccatgttat tccggcttcg
    20401 cgcgacacat tcctgaaaaa cggaagattg gtgaggggat ttattccctc attcctgtac
    20461 agcctgtcgg tattgggcat cgagccgtca tttttccttt ggcgaagggc attcttgttg
    20521 gggattgtca tcgaagcagt aacaagatac atatcaagat ccccgtcgcg gtcataatca
    20581 aaaaacactg cctgggttga atagcccctg tcagccaggc caaccgcagc ggcacattcc
    20641 gtgaaaaaaa gattatcagg gtcgccattg ctgatataaa gcatattggt gtagtcatag
    20701 tcgatattgc ctgctgcaca caggtaaata tcaagccatc cgtcattgtt gacatcggcg
    20761 aggcttactc ccgtaatcca tttgcccgcc gtattgaccc cgcactgcct ggtaacatca
    20821 gcaaacttaa gtccgccctt gttcagatac accctgcact cggtcatatt tgcaccgaat
    20881 acaatatcac tcagcccgtc attgtcgaga tcacccacag caagtccggc cccgttgtag
    20941 taatattcat agaagattgc attgtcagta tccgattcgg taagcctgtt tgcgaaggtt
    21001 atgccgcatt ctttcggatc aagcagcctg aacaaagtct ttgagctggc acgcttttta
    21061 aaccgttcat tctggcaaga tgagaagatt acaaaaaaga aaataataaa gaagaaaaaa
    21121 aacctgccgg atggtttaat catttaatgc ttgttggttt ggatctcttg ccataatgat
    21181 agtaaataaa actcaaaaaa agagctaccg gattaaaaaa tccggtagct ggctgtaaat
    21241 ttttcagtta tgctataaat tacggagatg tatacccggg attctggacc agggctttat
    21301 tcagtctcag atccttttgc ggaattggca gcaggtaatc ttcaatggaa taattctgca
    21361 gaaactcagg atctgtcacc ttatccagta tccgttttcg ctgaatgtcg aaccacctgt
    21421 catattcgaa acacagttcc cagcttctct gctggattat tgcttcatcg aattcctcct
    21481 ttgtcattga tgttgtaaag agcggatcgt caggattcgg gacacccccc gtagccctgt
    21541 tcactatctg gttaactgca tcgcaggctg cctgcgttgg cccgttcacc tggttctcgg
    21601 cttcggcaaa cagca
//
LOCUS       DAOOYG010000037        21561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2284_length_21561_cov_7.203339, whole genome shotgun sequence.
ACCESSION   DAOOYG010000037 DAOOYG010000000
VERSION     DAOOYG010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21561
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2284_length_21561_cov_7.203339"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /gene="mqnB"
                     /locus_tag="PK288_04985"
     CDS             complement(<1..589)
                     /gene="mqnB"
                     /locus_tag="PK288_04985"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HPM18308.1"
                     /translation="MAEAEVLRKIAVKNESGERFLLGNCIFDLLVTGVGCAATVWAIS
                     RWLFHNRRPDLVINSGIAGSFRDDIYLGQVVLPVSDCFADFGVETGTGFLTLAEAGLE
                     DPDGPPFSGGRLIAGNSFIDMSARRLRTVNAISVSTATGSTETRQRLVEKYDPDIETM
                     EGAAFFYACRSADVPFMAFRSVSNMVEPRDKSRWNI"
     gene            716..1129
                     /locus_tag="PK288_04990"
     CDS             716..1129
                     /locus_tag="PK288_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPM18309.1"
                     /translation="MIYITRRERFCAAHRMFREEWSDEENMKVFGKCSNSNWHGHNYV
                     LYVTVKGEPSEKLGFFININTLKRIIREKIVEKVDHKNLNIEVGFMKGKIATTENLAI
                     AIWNELKPEIEKEGAILHCIKICETENNSIEYYGE"
     gene            1119..1766
                     /gene="folE"
                     /locus_tag="PK288_04995"
     CDS             1119..1766
                     /gene="folE"
                     /locus_tag="PK288_04995"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPM18310.1"
                     /translation="MVNKIESVNNGNGLNTNGYNKIETWNAENIQELSEIYYRVLGLI
                     GEDPDREGLRGTPVRVAKALNFLMMGYNQNPCEIINSAKFREDYRQMVIVKDIDLYSM
                     CEHHMLPFFGKAHVAYIPNGYITGLSKIARVVEAFSRRLQVQERLTTQIRDCLQDCLN
                     PMGVAVVIEAQHMCMQIRGVQKQNSVTTTSAFTGIFLDNLNTREEFVHLIGSCYH"
     gene            1849..2730
                     /gene="fabD"
                     /locus_tag="PK288_05000"
     CDS             1849..2730
                     /gene="fabD"
                     /locus_tag="PK288_05000"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPM18311.1"
                     /translation="MKAYVFPGQGAQYPGMAKNLYEESSHARALFEKANSILGFKITD
                     IMFNGTDEDLKQTKVTQPAIFLHSVILALISGDSFRPGMVAGHSLGEFSALVANKTLS
                     FEDGLLLVSGRAAAMQKACELTPSTMAAVIGLDDAVVEEVCSQAGEEVVPANYNSPGQ
                     IVISGSNEGIDKAIELLRERGAKRVLKLAVGGAFHSPFMEPARLELEETIRKTRFTQP
                     VCPVYQNVTANPSTDPEEIKTNLVLQLTSPVKWTQCINNMIAGGATSFTEVGPGNVLQ
                     GLIKKISRDTVTDSIIL"
     gene            complement(2841..5813)
                     /locus_tag="PK288_05005"
     CDS             complement(2841..5813)
                     /locus_tag="PK288_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPM18312.1"
                     /translation="MDKIEGIQDLQRFEFTHTSFDLLMQRRIHRVLIICSNYDNYMLE
                     EDGRIDERVFNEYLSLNLKYPPSFVQTDNPEDAFRILETNKIDLVISMLSLRGMDVFA
                     MAREVKKRYANIPIVVLTYFSREVSLRLEGEDLSAIDYVFCWLGDSSLILAIIKLLED
                     KMNAEFDIENIGVQAIILVENSIRYISFFLPNLYRMVLQQSRDFQKEALNEHQRMLKM
                     RGRPKILFASNFNDALALYKKYQHNVLGIISDISYKRDGMIDENAGVELCKIVKADND
                     QVPFLLQSSSMVHKTIAEELGAGYINKSSKSLSMELRNFFIQNLAFGPFVFRNPETME
                     PIAIASDLHSLQQKLLTIPDKSLIFHATRNHFSKWLNARALFPIAQMFKYINTGDFET
                     TDEMRKFLYVAISSFRVGRGRGIIAKFDKTSYDEYQFFSRIGDASIGGKARGLAFINR
                     IIRNNKLYNKFPDVHITIPRTVVLSTDIFDEFMDHNNLYPVALSDLPDEEILNRFVSA
                     ELPGRVYHDFLAFLEVSKNVPMAVRSSSRLEDSRYQPFAGIYSTYMVPRHPNDQVMVQ
                     MISNAIKEVYASVYFKGSKAYMAATANVIDEEKMGIILQEVCGNKHGDLFYPTLSGVA
                     QSINYYPIGSEKAEDGIAKIAFGLGRLIVEGGLALRFSPKHPGKILQLSSVDTALRDT
                     QKEFRALDLRIESFVPSTDDGMNILTISINDANNAGAMKYAASTWDRNNNMLRDGYNA
                     SGRQIITFANVLQHKIFPLAEILSTLLEIGQNEMNSPIEIEFAANLETPSGTPKILNF
                     LQIRPIVNSDEPLKINIESIQPEKTILLSEAALGNGLFKGIRDIVYVRPESFNPSKNR
                     DLAGEIERFNNIFINKGEGYVLIGPGRWGSSDPWLGIPVRWPQISAARVIVESGLKNY
                     RIDPSQGTHFFQNLTSFRVGYFSVNPYIGEGYYDVAFLNSLQAEYEDEFIRHVRFDRP
                     LEIMIDGRSHNGLIMKPS"
     gene            complement(5826..9272)
                     /locus_tag="PK288_05010"
     CDS             complement(5826..9272)
                     /locus_tag="PK288_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPM18313.1"
                     /translation="MDSKRHITPEYSKLLHDQQERVKELGSINQTTRILKENRPVEET
                     LRQIALLLPSAWQYSGYTTARITFMHLQYESVDFRETRWRMAQEFETIDGEKGLIEIF
                     YTMEFREEYEGPFLKEERDLIINISSLIAGYLNSRKASDYIGVTGPTDGKEDDLVHVP
                     SKKLLQRFLDMHNAERDVFHDLQLFKVKEILLLANLYDAYSIEGEGRFADHILGEYHQ
                     LSLTSIPRITGVSGEEEAFDRLRTRHYDMIIIMIGVDRESPMELCRKIRDKYPYIPTF
                     LLLNNPADMEYIERQKESGIPFDNYFVWTGDSKVFFAMVSLLEDRVNVENDTRKGLTG
                     VILLVEDSADYYSSYLPMLYNLVMDQTRSLIEDVSADELYKVFKLRTRPKILLAPDWE
                     SAINIFSRYRNNLLCVISDIRFPRNGVMDDKAGFELIRYIKEELPSLPAVLQSSDPEN
                     VRYAFTLKSNFINKNSPGLLEDLKSFINHYLGFGHFVYRDNQGRQIAVAKTMKEFESF
                     LETVPEDSLAYHAHRNHFSLWLMARGEVKIAKLIYPMKVSDFGSLREMREFLIGIIRK
                     RRQEMNKGRVISFEAAAMIDETNVISLSTGSLGGKGRGLAFINSLIYSFGLGKLIPGI
                     NIRTPITAIARTDEFDLFMERNHLWNFVKGEKDFMAIQRTFLAGSLSYTLEKKLRAIL
                     GYFTRPLAVRSSSLFEDSMSQPFSGIFGTYLLPNNNPDPDIRLRQLSDAIKLVYASIY
                     SEEARAYFDAVRYKMEVERMAVVIQEVVGNQYEDVFYPHISGSAQSFNFYPIAHMKPG
                     DGLAVAAVGLGQYVMDGELSYRFSPAYPSLEIISQKDLYKNSQVWFYAVDLAKQDLNL
                     LEGEKAGLRRLEISSAERHGTISHSASVLNPDNDMITPGLDSSGPRVINFADILKYDY
                     IPLASTLRTVLDVVAEACGTPVEIEFAVDLNKDGSGNASFYLLQLKPLLGTGAGYNIA
                     ADSIDENKVVLLTRQSMGNGMISDITDIIYIEPERFDNKKTVQMAGEIAVLNEMMVRE
                     DRKYVLIGPGRWGSRDRFLGIPVVWAQISNARVIVELSLPDFHTDASLGSHFFHNVIS
                     MNVGYFSLHHESDGHIIDWNRLRSQVVIWKGEFFSHVRFREPLLIRMDGKKGMAVISV
                     NQ"
     gene            complement(9653..10672)
                     /locus_tag="PK288_05015"
     CDS             complement(9653..10672)
                     /locus_tag="PK288_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPM18314.1"
                     /translation="MAEENKLKYSPKDFKSDQEVRWCPGCGNHAVLSAVQRALAELDI
                     PKENYVFVSGIGCSSRFPYYMDTYGFHGIHGRASTIATGVKTANPGLSVWQITGDGDA
                     LAIGGNHFIHAVRRNVDINIILFNNEIYGLTKGQYSPTSEKGLVTRTSPYGTVEEPFS
                     VGELVIGAKGKYFARTIDANVTLSTQIYKDAARYKGTSVIEVLQNCVIFNDGVHENIS
                     GREVRDDRTLTLRHGEPMIFGKNNDKGLVLDGLKLKVVNLGDGYREKDLLIHDAYETN
                     PGIHYMIASMRYPEYPVALGVIRAAAGQTYEMAVEEQIRSVGKNSEIKCVADLLKSGS
                     TWKVG"
     gene            complement(10674..12518)
                     /locus_tag="PK288_05020"
     CDS             complement(10674..12518)
                     /locus_tag="PK288_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPM18315.1"
                     /translation="MGKKPQIIEREEVVIRFSGDSGDGMQLTGTLFSDTAALFGNDLS
                     TFPDYPAEIRAPQGTVGGVSGFQVHVGHKEVNTPGDLADVLVAMNPAAIKANAGWIKP
                     GGTIIYDENNFTEKNIEKAGYEKDPIKEEKLDNCIIIPAPVTSMVREALKDLNLEPKA
                     VLRTKNMFALGMVYWLFNRKFKYTDEYFEKKFKNKPDIVKANKIALRAGYYYAETIEA
                     LSPKYKILPAKLPKGVYRNISGNTAIAWGFLAAAEKAGLKLFIGSYPITPATGILEEL
                     ALRKDLGVISFQAEDEIAGICSALGASFTGRLAVTSTSGPGLSLKSEAIGLAVMAELP
                     IVIVDVQRGGPSTGLPTKTEQADLLQALYGRNGESPVVVLAASTPADCFHFAFEAARI
                     ALEHMTPVILLTDGYLANGTEPWRIPEMDELPVINPPFATPGDTPFLPYKRDEQKLVR
                     SWALPGTPGLEHRIGGLEKTIKGTVSYTPDNHELMVKTRAEKIMRVSGSIPDLKVQGD
                     ESGDLLVVGWGGKYGYIITAVRELQMEGYKVSMVNFNYINPLPGNVRDLFSRFKKILV
                     CELNLGQFAGYLRMKHQEFEYQQINKVQGQPFTINEIKQKCYEILKEE"
     gene            complement(12736..13536)
                     /gene="murI"
                     /locus_tag="PK288_05025"
     CDS             complement(12736..13536)
                     /gene="murI"
                     /locus_tag="PK288_05025"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPM18316.1"
                     /translation="MKEHPIGIFDSGVGGLTVANAIKQALPGESLVYFGDTAHLPYGD
                     KSAEAIRYYSGRITEFLLSHNSKVVLVACNSASASAFDSLKIRFQDRTILIDVIDPVV
                     DYLGRRNFRKVGIIGTKRTITSGTYEQKLRDKAPATEVVSMATPLLVPMIEEGFIFDD
                     ISNAIIRTYLSNNSLSEIEALVLGCTHYPIIKNQISRFFNFNVEVVDSGRNVSQILRE
                     ILEKNNLVNDSGKVHDQFFISDYTPYFEKIARMFFDGEINLQKADIWS"
     gene            complement(13618..14139)
                     /locus_tag="PK288_05030"
     CDS             complement(13618..14139)
                     /locus_tag="PK288_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPM18317.1"
                     /translation="MRRILIISVVIMISGLIGNQIMAQNIKFGHINRNELIQLMPEFD
                     SARSQLEKLNTELTNQLELLQVEYNNKAEAYIKESKNLSDLVRQTKEQELQDFQNRMQ
                     VFQQNASNQLQEKQVALFTPITEKADKAIKAVGKESSFFYIFDLSGGHVAYFDETKSV
                     DVLPLVKSKLGLK"
     gene            complement(14180..14695)
                     /locus_tag="PK288_05035"
     CDS             complement(14180..14695)
                     /locus_tag="PK288_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPM18318.1"
                     /translation="MKRSVLIISLVFLTAAFAAAQKYAFVDSEYIRKNIPAFNRAQEQ
                     LESLSKQWEKEVADGYAVVEQMYKSYQNDVVLLSQEMKTKREEAIIARENEVKALQNK
                     YFGMEGELFKKREELVKPIQDDILKAIKTIAAEGSYAAIFDTASGGNILFANPKFDIS
                     DQVLEKLGYKN"
     gene            complement(14818..17337)
                     /gene="bamA"
                     /locus_tag="PK288_05040"
     CDS             complement(14818..17337)
                     /gene="bamA"
                     /locus_tag="PK288_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPM18319.1"
                     /translation="MMMFIRRRVLFLSILLILVFQVGFSQEKSEFADYSTVEEYIIGG
                     VSVSGVRFLDANALIGLSGLRIGQEVSVPGDELTSAVRKLWDQGLFSDVRITILRQVA
                     DTVYLDISLQERPRISSIRYNGLKNSEAQDITGKINLPVGSQLTSYLINKSEKIIKDH
                     FIEKGFLNVSVEFIQKDDHSQPNHIMLTIDVDKKEKVKIAEISFEGNDKFPDKLLRRK
                     MKNTKKKNLNFFKASKYIDEKYLEDKESLYKFYNDHGYKDFRILKDSLYPVSDVRVAL
                     MIRVNEGRQYFLRNVDWVGNSVYKKEDLARRFNIEKGSVYNQSHIEDRLNGLSGAQDA
                     VSNLYQDYGYLFSRLTPVEAKIENDSVDLEIRIFEGDQAYLNNIIISGNTRTNEHVAR
                     RELYTLPGDLFSKDKIIRSIRQLGVLGHFDAEKISPTPLPDPANGTVDLLYKLEEKAN
                     DQFEISGGWGARMLIGTVGVRFNNFAMRNFFKLKEWRPYPSGDGQSLNIRAQSNGRYY
                     QSYNLSFMEPWLGGKKPNSFSASVYRSIMTNGLKKSNERYGSMIIDGASIGLGKRLAW
                     PDDYFSLYGELSYQRYKLHEYTFYRFLFADGTSNLFSFTTRLTRFSTSPNLIYPRSGS
                     TFTLSLQLTPPYSLISGTNMVGVSDRVKYNWIEFHKWLFKADYFYPISADDKLVLNAK
                     FAFGYLGHYNNAIGPSPFENFYLGGSGMSGYSLYGREIIALRGYTDGSLTPTDRKSGS
                     PAGNVYSKLTFELRYPVSLNQQATIYVLTFLEAGRAWYKINEYNPFKMNRSAGIGLRA
                     NLPMFGLLGIDWGYGFDKVPDPVTYPNANKGQFHFVLGQQF"
     gene            complement(17342..18088)
                     /locus_tag="PK288_05045"
     CDS             complement(17342..18088)
                     /locus_tag="PK288_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPM18320.1"
                     /translation="MSLKEQINPEKLPSHIAIIMDGNGRWAKQKGMDRIFGHRQGVSA
                     VRGVIEAAAELGIKYLTLYAFSTENWGRPDDEVTALMGIMVQSLNDETGTLIKNNIKL
                     TAIGDVDRLAGDVRNRFLETIRLTSASTGLTLVVAMSYSSRWEITEAARKLSADICSG
                     RISAGSVGPDEFGKYLATSEIPDPELLIRTSGELRISNFLLWQIAYTELYFTDILWPD
                     FSKEDFYRAIIDFQKRERRFGKTSEQVTEN"
     gene            complement(18091..18816)
                     /locus_tag="PK288_05050"
     CDS             complement(18091..18816)
                     /locus_tag="PK288_05050"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPM18321.1"
                     /translation="MHPIVSAFLNRIFLLFLFLLFPLSLSGQRNADFGLFGGTSSYIG
                     DINETRLFYAPLPAGGLFYRYNFNPRQALRTNIFIGGIRGKDSDFSNNFQRGRDASFS
                     GMVGEWALQYEFNFFPYSTQGKRWNYTPYLAAGAGISFINTSAKNPNSSVITYVPVIP
                     FSIGFKINVYKNLGLEAEYGFRKSFYDNFDGQKDLTDPSHHALLHNNDWYTFAGISVT
                     WKIYNKLAGCPAYSDVDEPRRKR"
     gene            complement(18819..19559)
                     /locus_tag="PK288_05055"
     CDS             complement(18819..19559)
                     /locus_tag="PK288_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18322.1"
                     /translation="MKRTALVVLLFSLIAGMCDAQLWKLKRWEAEFGIGPSFFFPDVG
                     GFSIGKNLAGLKDMGYRQTRFDINGSLRYRLSRTANLRLSMTYAMLHASDKRGSNENR
                     NYESTTNLFEPALVGEYYFIKNKYESSYLFIKGKGIQALLRSLDFYVFGGAGGAWYSV
                     KVNEALSARNMPTSGLAPVIPAGLGATLIYTPNLNFGLELGGRYVFSDYIDGYHSQFS
                     KANDVYYLMNVNITYKLKSGARGLPTLR"
     gene            complement(19689..20567)
                     /locus_tag="PK288_05060"
     CDS             complement(19689..20567)
                     /locus_tag="PK288_05060"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPM18323.1"
                     /translation="MLRSVAIYSKESNQKTTEGVSRLIREFSQRGIKIFQHQKHTIDS
                     DSIEAGRPVRFKDLESLPYLPDMILSVGGDGTFLETMLKVRKHGIPIAGVNTGRMGFL
                     ANIPDDEIGHSIDLLCQGEYEIIERSLLEIRSPADLFNNESSTALNEITVQKADPSMI
                     IINVFVDNIYLNTYWADGLIISTATGSTAYNLSVGGPILSPEDESIIISPISPHNLTI
                     RPIVISGESELRMTIEGRADAYLATCDFRSKRLSFDQAINIARSPEKLKTVMLRKRDF
                     YSTLRNRLMWGADKRN"
     gene            complement(20573..21238)
                     /locus_tag="PK288_05065"
     CDS             complement(20573..21238)
                     /locus_tag="PK288_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPM18324.1"
                     /translation="MIARDLISEVVPALKTSDSGQTALNWMEIFRISHLPIVNNQDFL
                     GLISDSDIYDMNQPEEPIGNHSLTLLKPYVTDDQHLFEVIGLASRLKLTVVPVLDSHN
                     HYKGVITATDLVRYLASISSIDQSGGILVLEITERDYSLSQIAQIVESNNIKVLSLYI
                     TSPPDSTRLEVTLKVNSTDLASLIRTFERYNYDVKTWITSDDSMDRFYSERFDLLMKY
                     LNI"
     gene            21341..>21561
                     /locus_tag="PK288_05070"
     CDS             21341..>21561
                     /locus_tag="PK288_05070"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPM18325.1"
                     /translation="MTKLSVNINKIATLRNARGGNIPNVKTAAINCELFGADGITVHP
                     RPDERHIRYRDVLEIRPVIKTEFNIEGYPS"
BASE COUNT         5850 a         5165 c         4443 g         6103 t
ORIGIN      
        1 ggatgttcca acggcttttg tctcttggtt caaccatgtt ggaaactgac cggaatgcca
       61 tgaacggcac atccgcactc cggcatgcat aaaaaaaggc tgctccctcc attgtttcaa
      121 tatccggatc atatttttcc acaagtctct gtctcgtctc agtagagccg gttgccgtgc
      181 tgacactaat ggcattaact gtccttaacc gccgtgcgga catatcaata aaactgttgc
      241 cggcaataag cctgcccccc gaaaacggcg gcccgtcagg atcttccagc cctgcttcgg
      301 ccagggtaag gaatcctgta ccggtctcaa cgccgaaatc agcaaaacaa tcggaaaccg
      361 gcagcaccac ctgccccagg tatatgtcgt cccggaaact tcctgcaata cccgaattaa
      421 tcacaaggtc aggcctgcgg ttgtggaata gccaacggct gatcgcccat actgttgcag
      481 cacatcctac tcctgtgaca agaagatcaa aaatgcagtt tcccaataaa aatcgttccc
      541 ccgattcatt tttcaccgca atcttcctga gtacttcagc ttcagccatg gttgctgtta
      601 caaataaaat attgatgtac atcgcattga actcatttaa acactaaata tatcgcattc
      661 aaatttaaaa gttcaactta tttggttatt attgcatgtt aattttcaag cccggatgat
      721 atatataaca cgcagggaac gattctgtgc cgcacacagg atgttcaggg aagaatggtc
      781 ggatgaggag aacatgaaag ttttcggaaa gtgctcaaat tccaactggc acgggcataa
      841 ctacgtccta tatgtaaccg ttaaagggga accttcagag aaacttggct ttttcatcaa
      901 tatcaacacc ctgaaaagaa taatccgtga aaagatagtt gagaaggtag atcataaaaa
      961 cctcaatatt gaagtcggat tcatgaaggg caagattgcc accactgaaa acctggccat
     1021 cgctatatgg aatgagctta aaccggaaat tgaaaaggaa ggagcaatac tacattgcat
     1081 aaaaatctgt gagaccgaaa acaactcaat tgaatactat ggtgaataag attgaatcag
     1141 tgaataacgg aaacggcctt aataccaatg gctacaacaa gattgaaaca tggaatgcag
     1201 aaaatataca ggaattatcc gaaatttatt acagggtttt aggacttatt ggcgaggatc
     1261 ctgaccgcga aggactcagg ggaactccgg tacgggttgc aaaggcactg aatttcctga
     1321 tgatgggata caatcagaat ccctgcgaaa taatcaattc tgccaagttc agggaagact
     1381 accgccagat ggtcattgta aaggatattg atctgtactc aatgtgtgag caccacatgc
     1441 tgccattttt cgggaaagcc catgttgcct atattcccaa tggatacata acaggcctca
     1501 gcaagatagc aagagtggtt gaagccttca gccggaggct gcaggtacag gagcggctca
     1561 ccacccagat cagggattgc ctccaggact gcctcaaccc aatgggagtg gcagtagtga
     1621 ttgaagcaca acatatgtgc atgcaaatca ggggtgttca aaaacagaat tcagtgacga
     1681 ccacatcggc atttacagga attttccttg ataaccttaa caccagggag gaattcgtcc
     1741 atctcatagg ttcatgttat cattaacatt ttacggtttc cctgacacct gtctgaattg
     1801 ttcattaatt atcttaatca cctggttctg ctgcaataat attactgtat gaaagcatat
     1861 gtttttccgg gccagggggc ccagtatccg ggaatggcta aaaatctata cgaagaatca
     1921 tctcacgcac gtgctctttt tgaaaaagcc aattcaatcc ttggattcaa aataactgat
     1981 atcatgttca atggtacgga tgaggacctt aaacagacaa aggtgaccca gcctgccatt
     2041 ttcctccatt ccgtcatcct ggctttgatt agcggcgatt ccttcagacc cggaatggtt
     2101 gcaggtcatt cactggggga gttttctgca cttgttgcaa acaagacact ctcgttcgaa
     2161 gacggccttt tgctggtttc aggcagggca gcagcaatgc agaaagcctg tgaattgaca
     2221 ccgtcgacaa tggcagctgt tatcggactc gatgatgcag ttgtcgagga ggtttgcagc
     2281 caggctggtg aagaagtagt tcccgccaat tataacagcc cgggacagat agtgatctca
     2341 ggttcaaatg aaggcattga caaggcaatc gaactgctca gggaaagagg agcaaaaagg
     2401 gtattgaaac tggcagttgg aggcgcattt cattccccgt tcatggagcc agcccgcctg
     2461 gaacttgaag aaaccataag gaaaacaaga ttcacacaac ctgtctgccc ggtttatcag
     2521 aacgtaacgg caaatccttc aactgatccg gaggaaataa agacaaacct cgtattacag
     2581 ctcacctcac ctgtaaaatg gactcaatgt atcaataata tgatagccgg cggggcaact
     2641 tcatttaccg aagtcggacc tggaaacgtt cttcaggggc taataaaaaa gatcagcagg
     2701 gatacggtga ccgacagcat cattctgtag cctttggaaa ttgcacagga aaacaattga
     2761 tccggataca tttaatcccg aaatttcctt tccggcaggg atctgatctt gtcacatacc
     2821 tgcgccggat accaatctgt tcatgaaggc ttcattatca atccgttgtg actccgaccg
     2881 tcaatcatta tttccaatgg tctgtcaaat ctcacatgcc tgataaattc atcctcatat
     2941 tccgcctgca aactgttcag gaatgccaca tcatagtaac cttccccgat gtaaggattt
     3001 accgaaaaat atccgaccct gaaggaagtg aggttctgaa aaaagtgggt cccctggctt
     3061 gggtcgatcc tgtagttttt caagcctgat tcaactatta ctctggctgc ggagatctgc
     3121 ggccacctta ccggtatacc cagccaggga tctgacgaac cccagcgtcc gggaccaatc
     3181 agcacatagc cttcaccctt atttataaat atattattga atcgttcaat ttcgcctgca
     3241 agatccctgt tcttcgatgg attgaacgat tctggcctta cgtagacaat gtcgcggatc
     3301 cccttgaaca agccgttgcc aagcgccgct tcggaaagca gaatcgtttt ttcaggctga
     3361 atactttcaa tatttatttt taaaggctca tctgaattga cgatgggccg gatttgaagg
     3421 aaattcagga ttttaggcgt tccggacggt gtttcaagat ttgcggcaaa ttcaatctct
     3481 atcgggctgt tcatctcatt ctgcccgatc tcaagtagtg tactcaatat ttcagcaaga
     3541 ggaaagatct tgtgctgcaa tacattggca aaagtaatta tctgccggcc ggaagcgttg
     3601 tatccatccc tgagcatgtt gttgttacga tcccaggttg aggccgcata tttcattgct
     3661 cctgcattat ttgcatcatt tatgctgatt gtgaggatat tcattccatc gtcagttgaa
     3721 ggcacaaagc tctctatcct gagatcgagc gccctgaact ctttttgggt atcccttaaa
     3781 gctgtgtcaa ctgatgaaag ctgaagtatt ttgccgggat gttttggtga gaacctcagg
     3841 gcaagccccc cttcaacaat gagcctgcca agcccgaacg caatttttgc aattccgtcc
     3901 tccgcctttt ccgatccaat cggatagtaa ttgattgact gtgcaactcc tgaaagcgtg
     3961 ggataaaata aatccccgtg cttgtttccg cacacttcct ggagaattat ccccattttc
     4021 tcttcatcaa taacatttgc agttgcagcc atataggctt tgcttccctt aaaatacacc
     4081 gacgcataaa cttccttaat tgcatttgaa atcatctgta ccattacctg gtcattgggg
     4141 tgccgcggga ccatataggt tgaatatatt cctgcaaaag gctgatatcg tgaatcttca
     4201 agcctgctcg acgatcttac cgccatcgga acgttctttg agacttccag aaacgccagg
     4261 aaatcgtgat aaacccggcc cggtaactcg gcgctcacga acctgttcag gatttcttca
     4321 tccggcagat ctgatagggc aacaggataa agattattgt gatccatgaa ttcatcaaag
     4381 atgtcagtac tgaggaccac agtcctggga atggttatgt gtacgtctgg gaacttgttg
     4441 taaagcttgt tgttcctgat aatcctgttt ataaatgcta gtccccttgc tttgcccccg
     4501 attgaagcat caccgatcct ggaaaaaaac tggtattcat cgtaactggt cttgtcaaat
     4561 ttagctatta tccctctacc ccttcccacc ctgaagcttg agatggcgac ataaagaaac
     4621 tttctcatct catcagttgt ttcaaaatca ccggtattga tatatttgaa catctgtgca
     4681 atgggaaaca gggccctggc attgagccat ttggaaaaat ggttccgggt tgcgtgaaaa
     4741 atcaggcttt tgtcgggtat cgtaagcaat ttctgctgaa ggctgtgcag atcggaagcg
     4801 attgcaatag gctccatggt ctccgggttc ctgaatacaa acggtccgaa agccagattc
     4861 tggatgaaaa aattcctgag ctccatcgaa agtgacttgg agctcttgtt tatgtaaccg
     4921 gcgccgagtt cctctgcaat cgttttatgt accatactcg acgactggag cagaaaaggc
     4981 acctggtcgt tatccgcttt cactatttta cacaattcga ccccggcatt ttcatcaatc
     5041 ataccgtcgc gcttgtagct tatatcagaa ataataccca gtacattgtg ctggtatttt
     5101 ttatacaatg ccagggcatc attaaaatta ctggcaaaca ggatttttgg cctccccctc
     5161 attttaagca ttctctgatg ctcattaaga gcctccttct ggaaatcgcg cgactgctga
     5221 agcaccatcc tgtaaagatt gggcagaaaa aaggaaatat agcggattga attttccacg
     5281 agaattatag cctggacacc tatattttca atgtcgaatt ccgcattcat tttgtcttcc
     5341 agaagcttga tgattgccag gatcagcgaa gaatcgccca gccagcaaaa tacatagtca
     5401 atggcactca gatcttctcc ttcaagtcgc agggatactt ccctcgaaaa ataagtcaga
     5461 acgactatcg gaatattggc atatctcttc ttcacttccc tggccatggc aaaaacatcc
     5521 atgcccctca gcgacaacat cgatataacc aggtcgatct tgttcgtttc aaggatcctg
     5581 aaagcatcct cgggattgtc tgtctgtaca aatgaaggcg gatatttaag attcagcgac
     5641 agatattcgt tgaaaaccct ctcgtcaatc ctgccatcct cttcaagcat ataattgtca
     5701 taattactgc agattatcag gaccctgtgt atccgtctct gcatgagaag atcaaatgaa
     5761 gtatgcgtaa attcaaatct ctgaagatct tgaattccct ctattttgtc catattcctg
     5821 ttggtttact gattaacgga aatcactgcc atacctttct tgccgtccat ccttatcagc
     5881 aggggctccc tgaatcttac atggctgaaa aactctccct tccagatcac cacctggctt
     5941 ctgagcctgt tccagtcaat gatatggccg tctgattcat gatgcagcga aaaatatcca
     6001 acattcattg atattacatt atgaaaaaaa tgagagccca gtgaagcatc ggtatgaaaa
     6061 tcaggaagtg aaagctcgac tatgaccctg gcatttgata tctgcgccca cacaacgggg
     6121 atcccaagaa acctgtcccg tgaaccccat ctgccggggc cgatcagaac atatttccgg
     6181 tcttccctta ccatcatttc attaagcact gcaatctcac cggccatctg tactgtcttt
     6241 ttattatcga atctctccgg ttcaatataa ataatatcag tgatatcgct gatcatcccg
     6301 ttccccatgc tttgccttgt caggagaaca actttatttt catcaatgct gtcagccgct
     6361 atattgtatc cggcaccggt cccgagcagc ggcttcagct gaagcagata gaatgaagca
     6421 tttcctgatc cgtctttgtt aagatcgact gcaaattcaa tctcaaccgg tgtcccgcac
     6481 gcctcggcca ccacatccag aacagtcctg agggtggatg ccagcggaat gtaatcatat
     6541 ttcaggatat cagcaaaatt gatcaccctc ggtcctgatg aatcaagccc gggagttatc
     6601 atatcattgt caggattcaa caccgatgcg gaatggctga tggttccatg cctttcagca
     6661 ctggaaattt caagcctccg caatcccgcc ttttcccctt caagcagatt cagatcctgc
     6721 ttggcaagat caacggcata aaaccagacc tgtgaattct tataaagatc cttctgagag
     6781 attatttcga gtgacggata agccggagag aacctgtaag acagctcgcc gtccataaca
     6841 tactgaccca gtccaactgc cgcaacggca agaccgtccc cgggcttcat atgggcaatg
     6901 ggataaaaat tgaaagactg ggccgatccg cttatgtgcg gataaaaaac atcctcatat
     6961 tgattgccta caacttcctg tatcacaact gccatcctct ccacttccat cttatacctg
     7021 acggcatcaa agtatgccct cgcctcttcc gaataaatgg atgcatatac aagctttatt
     7081 gcatctgaaa gctggcgaag ccttatatca ggatcaggat tgttattggg aagaagataa
     7141 gtaccgaaga tccctgaaaa gggctgactc attgaatctt caaacaggct cgatgatctg
     7201 accgccaggg gcctggtaaa atatcccagt atggcacgca gcttcttttc aagggtgtaa
     7261 ctcagactgc ctgcaagaaa agttctctga atagccataa aatccttctc tcccttaaca
     7321 aaattccaca gatgatttct ctccatgaaa aggtcaaatt catcggtacg tgcaatagcc
     7381 gttattggtg tcctgatgtt gatccccgga atgagttttc caagcccgaa actgtaaatc
     7441 agtgagttta tgaaagccag acctctgccc tttcctccca gcgaacccgt agacaggctt
     7501 atcacattcg tttcatcgat cattgcagcg gcttcaaaac tgataacccg ccccttgttc
     7561 atctcctgcc tccgctttct gataatgccg ataagaaact ccctcatctc acgaagactt
     7621 ccgaaatctg acaccttcat gggataaatc agttttgcaa tctttacctc accccttgcc
     7681 ataagccaaa gggagaaatg attcctgtga gcatggtagg cgagtgaatc ctccggaacc
     7741 gtttcaagaa aagattcaaa ttccttcatc gtctttgcaa cagctatctg ccgcccctga
     7801 ttatccctgt aaacaaagtg tccaaagccc agataatgat tgataaacga tttcagatct
     7861 tcaagaagac ccggagaatt cttgttaatg aaattggatt tcagggtaaa agcatacctc
     7921 acattctccg gatctgacga ctgcagtaca gcaggcaggc tcggaagctc ttccttgata
     7981 taccgtatga gttcaaagcc ggctttgtcg tccatgactc catttcgcgg aaaccgtatg
     8041 tcggaaatca cacaaagcaa attattcctg tacctggaaa agatgtttat tgcgctttcc
     8101 cagtccgggg caagcagtat tttcggacgc gtccggagtt taaacacttt atacagctca
     8161 tcagccgaca catcctcgat aagactccgg gtctgatcca tcaccaggtt gtagagcatg
     8221 ggcagataac tggaataata atcagcggag tcttcgacaa gcagaataac cccggtaaga
     8281 cccttccggg tgtcattttc aacgtttacc ctgtcctcga gcaaggaaac cattgcaaaa
     8341 aacacctttg agtcacctgt ccatacaaaa tagttgtcga aaggaattcc tgattccttc
     8401 tgcctttcaa tatactccat gtcggccgga ttgttcagga gcaggaaagt aggaatatac
     8461 ggatatttgt ccctgatctt tctgcagagc tccatagggc tctccctgtc cacaccaatc
     8521 attataatga tcatatcata atgcctggtc ctcagcctgt cgaaagcttc ctcctcgccc
     8581 gaaacaccgg tgatcctggg aatggaggta aggctcaatt gatggtactc acccagtata
     8641 tggtctgcaa aacgaccttc cccttcaata gaatatgcgt cataaaggtt tgcaagcagc
     8701 agaatttctt taaccttgaa aagctgaaga tcatggaaaa catcccgttc tgcattatgc
     8761 atgtcgagaa atctctgcag cagcttttta cttggtacat gtaccaaatc atcttctttc
     8821 ccgtccgtcg gaccggtgac cccaatgtaa tcgctggctt tacggctgtt caggtatccc
     8881 gcaatcagac ttgaaatatt gatgatcaga tcccgttcct ctttcaggaa aggtccttcg
     8941 tattcttccc tgaactccat ggtataaaag atttcaatca agcctttttc accatcgatg
     9001 gtttcaaact cctgcgccat tctccacctg gtctcccgga aatcgaccga ttcatactgc
     9061 agatgcataa aggttattct tgcagttgta tatccggaat actgccaggc tgaaggcaaa
     9121 agcaatgcaa tctgccgcag cgtctcttct acgggccggt tttccttgag gatacgtgta
     9181 gtctggttta tggaacccag ctctttgacc ctctcctgtt gatcgtgaag caacttgctg
     9241 tattcaggtg ttatgtgacg cttggaatcc attggataca ctatatgcca gttgatcaga
     9301 atttaaaatt agtaaaataa aggcttcacg gtaagtgatt cttcaatttc atcgccaccc
     9361 gtatcatctc cattccggca tatacaggta atgcccatga gagcagggtg tatggaagga
     9421 atcttaagag aaaaaaccgg aaaaaattgc tgaaacaacg atcaatgtaa catatatgat
     9481 gtatatcaat gattatattg atatatgtca tgtaatgaat taatgttgat caatgttttg
     9541 ttgattccgt attccggcaa ttgcagtatc acgccatctt accggtttca gaaagaattc
     9601 cggtaagttt aacaagatca ttatggcagg tgagctccgg gcctaattgt tttcagccga
     9661 ctttccaggt tgaaccgctt ttcagaagat cagccacaca tttgatttcc gagttttttc
     9721 caacggagcg aatctgttcc tcaacagcca tttcataggt ctggccagct gctgcacgaa
     9781 tcacccccag cgctaccgga tattccggat atctcatgct ggcaatcata taatgtattc
     9841 ccggattggt ttcatatgca tcatgaatga gcagatcctt ttcgcggtat ccgtctccca
     9901 ggtttaccac tttgagtttc aaaccgtcaa gaacaagacc tttgtcgttg ttttttccga
     9961 atatcatcgg ttctccatgc ctgagggtca gcgttctgtc atccctgact tccctgccgg
    10021 aaatattttc atgcaccccg tcgttaaata ttacacaatt ctgtaatact tcaatcactg
    10081 aagtgccttt gtacctggca gcatccttgt atatctgcgt tgacaatgtt acattggcat
    10141 cgattgtacg ggcaaagtat tttccttttg cgccaattac cagctctccc actgaaaaag
    10201 gttcctcaac ggttccgtaa ggtgatgttc tggtaaccaa gcccttttct gaggttggtg
    10261 aatactgtcc ttttgtaagc ccatatatct cattgttgaa gagtattata tttatatcaa
    10321 cattcctcct gacagcatgg atgaaatgat ttcccccgat cgccagggcg tcgccatcgc
    10381 ctgtgatctg ccataccgaa agcccggggt ttgcagtttt cactcccgtg gctattgttg
    10441 aagcacgtcc gtgaatgcca tggaacccat aggtatccat ataataaggg aaacgtgaag
    10501 agcatccgat acctgaaaca aaaacataat tttccttcgg gatatcaagt tctgcaaggg
    10561 ctctctgaac agcagaaagc actgcatgat tcccgcatcc cggacaccat ctgacctcct
    10621 ggtcgctctt aaaatccttc ggagaatatt ttaatttatt ttcttctgcc atattattcc
    10681 tccttcagta tttcataaca tttctgcttg atctcattta ttgtaaaggg ctgaccctga
    10741 actttattta tctgctgata ttcgaattcc tggtgtttca tccgcagata gccggcaaat
    10801 tgtccaagat tcagctcgca cacgaggatt ttcttaaatc tgctgaacag atccctgaca
    10861 tttccgggca ggggattgat atagttgaaa ttaaccatgc taaccttata gccttccatc
    10921 tgtagttccc gcactgctgt aataatgtag ccatacttcc cgccccatcc cacaacaagc
    10981 agatcgcccg actcatcgcc ctgcacctta aggtcgggga tactgccgga aaccctcata
    11041 attttttctg ctcttgtttt aaccatcagt tcatggttgt cgggggtata ggagaccgtt
    11101 ccctttattg tcttttccag tcctccaatt ctgtgctcca ggcccggggt tccggggagt
    11161 gcccatgaac gcaccagttt ctgctcgtcg cgtttatatg gcagaaaggg agtgtcaccc
    11221 ggagttgcga atggaggatt gatcaccggc aattcatcca tttccggtat tcgccatggc
    11281 tcggtgccgt tggccaggta gccgtcagtg agaagaatta cgggagtcat atgttcgagg
    11341 gcgatcctgg cggcttcaaa cgcgaaatga aagcagtcgg caggtgtgct tgcagccagt
    11401 acaaccacgg ggctctcccc gttacgaccg taaagcgcct gcagaagatc tgcctgttct
    11461 gtttttgtag gaagtccggt tgatggccct cctctttgta catcaactat cacgatgggg
    11521 agttctgcca ttaccgcaag acctatggcc tccgatttaa gcgaaagtcc gggtccggaa
    11581 gtggaagtga ccgccagccg tccggtaaaa ctggcaccaa gtgctgaaca gataccggca
    11641 atttcatctt ctgcctggaa gctgataacg cccagatctt tcctgagagc cagttcctca
    11701 agtattcctg ttgcaggagt aatgggatag gaacctataa aaagcttcag gccggccttt
    11761 tctgcagccg ccaggaatcc ccaagcaatg gcagtgttac cgctgatatt acgataaacg
    11821 cctttgggaa gctttgccgg aagtatcttg tatttaggac tcaatgcctc aatggtttcg
    11881 gcgtaataat aacccgcccg caaggcaatc ttgttggctt taactatgtc aggtttgttt
    11941 ttgaatttct tctcgaaata ttcatcggta tatttgaact tacggttgaa aagccagtaa
    12001 accatgccca gggcaaacat attttttgtc cttagcacgg ctttgggttc aagattaaga
    12061 tctttcaggg cttcccttac cattgatgtt acaggagcgg gaataataat gcagttatcg
    12121 agcttttcct cttttatcgg atctttttca tatccggcct tttcaatgtt tttctctgtg
    12181 aaattatttt catcataaat tattgtacct cccggcttga tccaccctgc attggctttg
    12241 attgcagcag gattcattgc tacaagaaca tcggccagat ccccgggcgt attcacctcc
    12301 ttatgtccta catgaacctg gaaccccgat actccgccca cggtaccctg aggtgctctt
    12361 atctccgccg ggtaatcggg aaaagttgaa aggtcattgc caaacagtgc ggcggtatcg
    12421 gaaaaaagcg ttccggtaag ctgcattccg tcgcctgaat cacccgaaaa ccggataaca
    12481 acctcttccc tctctattat ctgtggtttt ttccccatcg tcgttgtata ttaagaatta
    12541 aatcctgttt atataccaac tgtttcctct atgcaaaata aatcttcaaa gaccaaaata
    12601 tcaaataatt attgctgaca tcaaatgata ttgatcatac tatttcacag agtttctcac
    12661 cggatttgcc aaaatccggc atttccggga tccgggaaac cataatgtca ggaagggagg
    12721 caacagagat ggaattcagc tccagatgtc agctttttga agattaattt ctccatcaaa
    12781 aaacattctg gcaatttttt caaaatacgg ggtataatcg gaaataaaaa actgatcatg
    12841 aactttgccg gaatcattca caagattatt tttctccagt atctcacgaa ggatttgcga
    12901 cacatttctt cccgagtcga caacttcaac gttaaaattg aaaaacctgc tgatctggtt
    12961 ttttattatc ggatagtgtg tacaacccag caccaatgct tcgatttctg ataatgaatt
    13021 atttgaaaga taagtccgta taatggcgtt acttatatca tcaaaaataa atccttcctc
    13081 aatcatgggc accagcagag gtgtagccat tgaaaccacc tctgtcgcag gcgccttatc
    13141 cctcagcttc tgttcatagg tgccggaggt aattgtccgc ttggttccga ttatcccgac
    13201 tttcctgaaa ttgcgcctgc caagataatc tacaacagga tcaataacat ctataaggat
    13261 cgttctgtcc tgaaaccgga tcttcagcga atcaaatgca gatgctgaag ctgaattgca
    13321 tgcaacgaga accaccttgg aattatgact gagcaggaac tccgttatcc tgccggagta
    13381 gtaacggata gcctccgctg atttatcacc gtatggaagg tgggcagtgt ctccgaaata
    13441 gacaaggctt tcccccggta acgcctgctt gattgcattg gctactgtca gccctccgac
    13501 tcccgaatca aaaatcccga taggatgttc tttcacctga aaatatttta aaacatgagg
    13561 ctgtaccgaa aaatacctta tttctgatac agcctccgga gtttatttgc taatattcta
    13621 tttcagtcca agtttggact ttacaagcgg caaaacatcg acgctttttg tttcgtcaaa
    13681 atatgccaca tgtccgccgg acaggtcgaa gatatagaaa aaactactct ctttgccgac
    13741 agccttgatg gctttgtcgg ccttttccgt tatgggtgtg aacaatgcga cctgtttttc
    13801 ctgcaactgg tttgatgcat tctgctgaaa gacctgcatc ctgttctgga aatcctgcaa
    13861 ttcctgctct tttgtctgac gtaccagatc cgagagattc ttgctttcct taatataggc
    13921 ttccgcctta ttgttatatt caacctgcag taattcaagc tggtttgtaa gctccgtatt
    13981 gagtttctcg agctgggatc ttgcactgtc aaattccggc atcagctgga tgagttcatt
    14041 cctgtttatg tgtccaaact taatattctg cgccataatc tgatttccga tcagccctga
    14101 aatcataatt acaacactga taatcaaaat tcttctcatg attgtaatcg ctttaaattt
    14161 tccgcctaaa aataattaat taattcttat aacctaactt ttcaagaacc tggtcgctga
    14221 tatcaaactt cggattggca aaaagaatat ttccccctga tgccgtgtca aaaattgccg
    14281 cataactgcc ttctgcggca attgtcttaa tagccttcag aatgtcatcc tgaatcggct
    14341 taacgagttc ttcacgcttc ttaaacaatt ctccttccat cccgaaatat ttgttctgaa
    14401 gagcttttac ctcattttcc cttgctatga tggcttcttc ccttttggtc ttcatctcct
    14461 gcgaaagaag cacaacatca ttctggtaag atttatacat ctgttcaaca actgcataac
    14521 cgtcggcaac ctctttctcc cattgctttg agagactctc aagctgttcc tgtgcccggt
    14581 taaaggcagg tatgttcttc ctgatatatt cagaatcgac aaaggcatat ttctgggctg
    14641 cggcaaatgc agcagtcagg aaaaccaagc ttattattaa aactgatctt ttcatgtgaa
    14701 aataaattaa tatactactg aagattaagt gattcaaatg gaacccacat gaattttatt
    14761 ttcatgcttg tttgtgattt tgtatcatgt ggatttcata tcaatgattt ttttacatta
    14821 aaactgctgg cccaatacaa aatggaactg gcccttattc gcgttaggat aggttacagg
    14881 atcaggtacc ttgtcaaatc cataacccca gtcaataccc aggaggccga acatcgggag
    14941 gttggccctg agaccaatac cggcagaacg attcattttg aatggattgt attcgttaat
    15001 cttgtaccag gcccttcctg cctcaaggaa agtaagcaca tatattgtgg cctgctgatt
    15061 gagggaaaca ggatatcgaa gttcaaaagt cagctttgaa tagacatttc ccgccggtga
    15121 acctgatttc ctgtcggtgg gggtcaaaga tccgtcggtg tatcccctca gtgctataat
    15181 ctcacggcca taaaggctgt agccgctcat tccgcttccc ccgaggtaga aattctcaaa
    15241 cggactcggt ccgatggcgt tgttgtaatg cccgaggtag ccaaatgcaa atttggcatt
    15301 tagcaccagt ttgtcgtctg cagaaattgg atagaaatag tcggctttaa agagccattt
    15361 atggaattca atccagttgt atttaaccct gtcactgact ccgaccatgt ttgtaccgct
    15421 gatcagtgaa tacgggggag taagctgaag tgaaagggtg aaggttgagc cgctcctggg
    15481 atagatcagg ttgggacttg ttgaaaacct tgtgagcctg gtggtaaagc tgaacaggtt
    15541 tgatgttccg tcagcgaaca gaaacctgta aaaggtatat tcatggagtt tgtacctctg
    15601 atagctcaat tcaccgtaaa gggagaaata atcgtcgggc caggcaagcc ttttgcccag
    15661 accaattgaa gccccgtcaa ttatcatcga tccgtatctc tcgttgcttt tcttcaggcc
    15721 gttggtcata atcgatctgt aaacagaggc tgaaaaggaa tttggttttt taccgccgag
    15781 ccatggctcc atgaacgaca gattgtacga ctggtagtaa cggccgttcg actgggccct
    15841 tatgttaagc gactgtccgt cgcctgaagg atagggcctc cactccttca gcttgaaaaa
    15901 attcctcatc gcgaagttat tgaacctcac cccgactgtt cctatcagca ttcgggcacc
    15961 ccatccccct gaaatttcaa actgatcgtt agccttctct tcaagcttgt aaagcagatc
    16021 gaccgtaccg ttggcgggat ctggcaaagg tgtcggactg atcttttcag catcgaaatg
    16081 ccccagaacg ccgagctgcc ggatcgacct tatgatcttg tccttgctga acagatcacc
    16141 gggaagggtg taaagctccc ttcgggcaac atgttcattg gttcttgtat tgccggatat
    16201 gattatgtta ttcaggtaag cctggtcgcc ttcgaatatc cttatctcca ggtcaaccga
    16261 atcattttcg atttttgctt ccacaggggt gagcctcgaa aacaggtaac cataatcctg
    16321 atataaatta ctgacggcat cctgtgcacc gctaagccca ttgagcctgt cctcgatatg
    16381 tgactggttg tagaccgatc ccttttcaat attgaatctt cttgcaagat cttccttttt
    16441 ataaacactg ttacccaccc agtcaacatt cctgagaaaa tactgacgcc cttcattaac
    16501 cctgatcata agtgcaaccc tgacatccga cacaggataa agggaatcct tcaggattct
    16561 gaagtccttg tacccatgat cattatagaa tttgtaaaga ctctccttgt cttcgagata
    16621 tttttcatca atatatttcg aggccttgaa aaaattcagg ttcttcttct tggtgttttt
    16681 catcttccgc ctcagcagct tgtcagggaa tttgtcattg ccttcgaaac tgatctccgc
    16741 tatttttact ttctcctttt tgtcaacgtc aatggtaagc attatgtggt ttggctggga
    16801 atggtcatcc ttctgaatga actcaactga aacattcaga aatccctttt caataaaatg
    16861 atcctttatg atcttttccg acttatttat taggtaagag gtaagctgtg aaccaaccgg
    16921 aaggtttatc tttcctgtaa tatcctgggc ctccgagttt ttcagtccat tatatcttat
    16981 ggatgagatc ctcggtcttt cctgaaggga tatatcgaga taaaccgtat cagctacctg
    17041 cctgagtatt gtgatcctta catcggaaaa aagtccctgg tcccataatt ttctgactgc
    17101 acttgtgagt tcatcacccg gcacacttac ctcctgacct attcttaatc ccgatagtcc
    17161 tatgagtgca ttggcatcaa gaaacctgac ccccgaaact gatacgcccc cgatgatgta
    17221 ttcttcaacc gttgaataat cggcaaattc tgatttttcc tgtgaaaaac caacctgaaa
    17281 aaccaggatc aataatatag acaggaaaag taccctcctc cttataaaca tcatcatagc
    17341 gtcaattttc cgttacctgt tcacttgtct tgccaaatct tctttctctt ttctggaaat
    17401 caattatcgc cctgtaaaaa tcctccttgc taaaatcagg ccaaagtata tctgtaaaat
    17461 acaattccgt ataagcaatc tgccagagca ggaaattact gatccttaac tcgccgctgg
    17521 ttcttatcag gagctccgga tccggaattt cggatgtggc taaatatttc ccaaattcat
    17581 ccgggccaac tgaaccggca cttatcctgc cgctgcaaat atcggctgaa agttttctgg
    17641 ccgcttctgt aatttcccat ctcgaactat agctcattgc tacaaccagg gtcaatccag
    17701 tagatgcaga agtcagcctg atagtttcca ggaacctgtt tcttacatcg cccgcaagcc
    17761 ggtcaacatc accaatcgca gtcagcttta tattattctt tatcagcgtg cctgtttcat
    17821 cattcagcga ttgcaccata attcccataa gtgcggtgac ttcatcatca ggtctccccc
    17881 agttttccgt tgaaaaagcg taaagagtca ggtattttat tccgagttct gccgcagctt
    17941 ctatcactcc ccttacagca ctcaccccct gcctgtgtcc gaatatcctg tccattccct
    18001 tttgctttgc ccatcgaccg ttaccatcca taattatagc aatatgggaa ggaagttttt
    18061 caggattaat ttgttctttc aacgacatac ctagcgtttt cttcttggct catcaacatc
    18121 actgtaagca ggacaaccgg ctaacttatt gtatatcttc caggtaaccg atatgccggc
    18181 aaatgtgtac cagtcattat tatgcagcaa tgcatgatgt gatgggtcag ttaaatcttt
    18241 ttggccgtca aagttatcat aaaaactttt acgaaaacca tattcggcct ccaaacccag
    18301 gtttttatat acgttgatct taaaaccgat tgaaaacggg atgacgggaa cataagtaat
    18361 cacactgcta ttcggatttt tagcagatgt attgataaaa gaaattcctg cgcctgcggc
    18421 caggtatgga gtgtaattcc acctcttacc ctgcgtggaa taagggaaaa aattaaattc
    18481 atactgcagg gcccattcac caaccatgcc cgagaatgag gcatccctcc cccgctggaa
    18541 attgttgctg aaatcactgt cctttccgcg tattcccccg atgaaaatat ttgtcctcag
    18601 ggcctgcctc gggttgaaat tatatcggta aaaaagacct cccgccggca ggggggcata
    18661 aaaaagccgg gtctcattga tatcgccaat ataggatgag gttcccccga atagcccgaa
    18721 atcagcattt cgttgaccag acaatgaaag gggaaacaat aaaaacagaa aaagcagaaa
    18781 tatcctgttt aaaaaagctg acacaatggg gtgcataatc atctcaaagt aggcaatccg
    18841 cgggcaccag acttcaactt ataggtaata ttcacattca tcaggtagta aacgtcattc
    18901 gccttggaaa attgcgagtg atatccgtca atgtaatctg agaaaacata tctgccgccc
    18961 agttcaaggc caaagttcag gttgggagta taaataagcg tcgcgcccag gcctgccgga
    19021 ataaccgggg caaggccgct ggttggcata ttcctcgcac taagggcttc attgaccttt
    19081 acgctgtacc aagcaccacc ggcgcctccg aaaacataga agtcaagtga tctcagcagg
    19141 gcttgtatcc ctttcccttt aatgaacaga taactgctct catatttgtt tttaataaag
    19201 taatattctc ccacgagagc cggttcaaaa aggtttgtgg ttgattcgta attcctgttc
    19261 tcatttgaac ctctcttatc agaagcatgc aacatagcat aggtcatgct aaggcgtaaa
    19321 tttgcggttc tcgacaacct gtatcgcaaa ctgccgttta tgtcaaacct ggtttgtctg
    19381 taacccatat ctttaagccc tgccagattc tttcctattg aaaaccctcc gacatccgga
    19441 aagaaaaaag aaggaccaat accaaactca gcttcccacc tctttaattt ccaaagctgc
    19501 gcatcacaca tgcctgcaat cagggagaat aatagaacaa cgagggcagt tcgtttcata
    19561 acgacacttt ttctgattgt aagtacaaat atagtaatct attgcaaagg gcagaaatat
    19621 tcaggactaa aattagctgt gaaggccgcg gttcatttac tggtctgatc tctgaaaaat
    19681 gtttcatgtc agtttctctt atctgctccc cacatcagcc tgttccttaa tgtactgtaa
    19741 aagtcccttt tcctgagcat cacggttttt aacttttcag gggatcttgc tatattaatt
    19801 gcctggtcaa atgaaagccg ttttgatctg aaatcgcatg tggccaggta tgcatccgct
    19861 ctgccttcta ttgtcattct cagttcactt tcaccggaaa tgacaatcgg cctgattgtc
    19921 agattatgag gggagatggg agatattata atgctctcat cctccggcga caggatgggt
    19981 cccccaacac tcagattata ggcggttgaa cctgtggccg ttgaaattat caggccatcg
    20041 gcccagtagg tgttaagata aatattgtcc acgaaaacat ttatgatgat catgcttgga
    20101 tcagctttct ggacagttat ttcattcaat gccgttgaag attcattgtt gaacagatca
    20161 gccggtgacc tgatttcgag aagtgatctt tcgatgatct catattcccc ctggcacagg
    20221 agatcgattg aatggccaat ttcgtcatcg ggaatatttg caagaaatcc catcctgcct
    20281 gtatttactc cggcaatcgg gatgccatgc ttcctgactt tcagcattgt ttccaggaag
    20341 gtgccgtcac ctccgacgct tagtatcatg tcgggaagat acggaaggct ttcaagatct
    20401 ttgaacctca caggcctgcc tgcttcaatg ctgtctgaat caatcgtatg cttctggtgc
    20461 tgaaatattt ttattcccct ctgtgaaaac tcccttataa gcctgcttac cccttcagtg
    20521 gttttctggt tactttcctt gctgtatatt gcaaccgatc tcagcatgat tgtcagatat
    20581 tcaaatattt cattaacagg tcgaaccgct ccgagtaaaa tctgtccatt gaatcatcgg
    20641 aggtgatcca ggtcttaacg tcataattgt atctctcgaa agtacgtatg agcgatgcca
    20701 gatcagtgga gtttactttc agtgtcacct caagtctggt tgaatcgggc ggcgaagtaa
    20761 tatacaaact aagcaccttg atattgttgc tctcaacaat ctgggcaatc tgtgagagcg
    20821 aatagtccct ttccgtgatc tcaagcacaa gaatgccgcc agactgatca atcgatgata
    20881 tagaggcaag ataacgtaca agatcggttg cggtgattac tcccttgtag tgattatgac
    20941 tatcgagcac cggcactaca gttaacttga gccgtgaggc aaggccaatt acctcaaaaa
    21001 gatgctggtc gtccgtaaca taaggcttga gcagggtcag cgaatgatta cctatgggct
    21061 cttccggctg attcatgtcg taaatgtcgg agtcggatat caggccgaga aaatcctggt
    21121 tgtttactat tggcaggtgc gatatcctga aaatttccat ccagttcagt gccgtctgcc
    21181 ctgaatcgga ggttttaagc gccggtacaa cttctgatat gagatcacgg gcaatcatta
    21241 tgactggcaa aatgtttaaa tttcagataa aataacaaaa aaaaaaccag ttatcgtatt
    21301 tttacatcaa ataacaatac ggtaatataa agcaattatt atgacaaagc tgagcgttaa
    21361 tatcaacaag atagcaacac tgaggaatgc aaggggagga aatatcccca atgtaaaaac
    21421 tgctgccata aactgcgagc tgtttggtgc agacggcata acggttcatc ccaggccgga
    21481 tgaaagacat atcaggtaca gggatgtgct tgagatccgg cccgtgataa aaaccgaatt
    21541 caatatcgaa ggatatcctt c
//
LOCUS       DAOOYG010000038        20370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2492_length_20370_cov_6.684322, whole genome shotgun sequence.
ACCESSION   DAOOYG010000038 DAOOYG010000000
VERSION     DAOOYG010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20370
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2492_length_20370_cov_6.684322"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PK288_05075"
     CDS             <1..747
                     /locus_tag="PK288_05075"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPM18326.1"
                     /translation="IIREKGVNGDREMAYALWLAGFDVRDVHMTDLMTGRETLDDLNM
                     IVFAGGFSNSDVLGSAKGWAGAFRYNEAAGKALSTFFSRPDTLSLGVCNGCQLMTELG
                     LIYPGHEQRPELLPNSSGKFESGFISVKIPENRSVMLGNMSGMELGVWVAHGEGRFNL
                     PYPEEKYHIALKYSSSTYPANPNGSAYDVAGLCSDDGRHLIMMPHPERAFFPWQCPYY
                     PNERKQDDVTPWIKAFINARKWIEKTLSGS"
     gene            complement(734..1276)
                     /locus_tag="PK288_05080"
     CDS             complement(734..1276)
                     /locus_tag="PK288_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18327.1"
                     /translation="MKSRIIVSVAIGCMFLWSCGNKSRSIDQANEGNTVIQQEDGSLV
                     LNIDKAACYNDISNPASNTAEWKVVIAKPGRFKVWLTSATRDTTDLSYVNSVKVTFLD
                     SFLEVDPVCDKVVKNSEDISYPYYRADSYLGSFYVLEAGAYNLQVISEKVLPREAMNQ
                     HSGFTEDTRLMSLLLTPMTR"
     gene            complement(1424..3334)
                     /locus_tag="PK288_05085"
     CDS             complement(1424..3334)
                     /locus_tag="PK288_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPM18328.1"
                     /translation="MKLEPFIIPFNIGLYFILLYAVARSVIWYRDLSRPDKLRLQRGF
                     FGRAFGQSLREIFMESLIHRKILKTNFRLGYMHMSLAFGWFLLILFGTIEADIFGQKH
                     LNPPYKAIFFRFFNPAHGKTPFESFYAFLMDLILAFILSGLLLAIFKRFWPGTVGMKK
                     TTRHRLPDKVALASLWLIFPSRLIAESLTSGAYGTGSFLTGTLGAQLASFLPASELAY
                     PFWWLYSLSLGTFFILLPLTRYMHIPTELFLIFMRNSGIRTGDKAGTFSDVEVHSCSS
                     CGICIDACQMSFSAGINDMQSVYLLKAIRNNDKTNYTAFNCLLCGRCDVKCPVGIELS
                     PVRMIQRRAGDPVKERQSLFKAYFPDRKKNTRPRPVSTPSYDYLPSGSKAKASVLYFA
                     GCMTHLTPAIKNAMTRILDASGLEWLFMDEHAGACCGRPLMLAGQDREARELINYNSQ
                     IILDAGADILVTSCPICFKVFRESYYLDAEVMHHSQFISKIIDEDLVRMNFLHNRVTY
                     HTPCDLGRNSGVYEEPKQVLMHLARLEMTGYDDENALCCGGSLGNIMLSQEKRKSIAA
                     DAAKILTASDPDFLVTSCPLCKKTLANATSTKVVDIAELAEMALINEPVEKKSIPGYV
                     KMHEPSRFQHGF"
     gene            complement(3324..3668)
                     /locus_tag="PK288_05090"
     CDS             complement(3324..3668)
                     /locus_tag="PK288_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPM18329.1"
                     /translation="MDKFGFSLSRARVIDYDKNDRRIAEYIYEHEPSFRLCIECGACA
                     ATCTAGNFTEFSLREFNVLLKRGENDKVRARLKKCMFCGKCILACPRGVNTRNVIFLA
                     RQAFRIFDGNET"
     gene            complement(3661..4698)
                     /locus_tag="PK288_05095"
     CDS             complement(3661..4698)
                     /locus_tag="PK288_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM18330.1"
                     /translation="MSAHVVVAGGGAAGIEAAGMLARSGIRVTLIEKETEPGGHLKGW
                     YHLFPDRRDSREVISYLSRLISHENIDFIPGETVAKIDRKGLSFSVTTGKGGSFTADA
                     VLIATGFDMFRCERKEEYGYGIYDNVITSAELEVMFRNGEIKTVGGIVPSKLGMIHCV
                     GSRDEKVGNLYCSKLCCVTAVKQAMEIKEYISDARIFCFYMDMRMGGAMYEELYREAQ
                     EKYGINFIRGKVSEVSVSIDNRLIVKSEDTLAGRPLKMEVDMLVLMAGMEISDGGKKL
                     ALASGLKTGENRFLASADNHYGSNRSHISGIFYAGTCTAPMNVTDTISHARGAAAEII
                     DYLRNTGRHNG"
     gene            complement(4700..5761)
                     /locus_tag="PK288_05100"
     CDS             complement(4700..5761)
                     /locus_tag="PK288_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPM18331.1"
                     /translation="MESEGSERIWSDYQKEIADDHFFYVRSCIRQSFFPGSEKFFLDF
                     LRDKLGKDVFENPSHTTCTGIGYHADIVPFQTIMTVIARHYALMKEAGYKNIAISCVT
                     SFGIYAEILETWKHFPEELEKTREYLYKATGREFDLPENIAHTSDIIYKFRNEIAANA
                     KYLLVNRQTGEPLKVVEHIGCHYAKIFPKYGVGGAEFPKVLSGMIEAWGGEVVDYPER
                     RHCCGFGFRQYLVQANRGYSLSNTAKKFSSMKPFKPDLVLTNCPGCTMFMDKWQYTIK
                     EMDGTTFGVNGESIPVLTYEELAGLVLGYNPWDLGLQYHMVQSEPLLRKMGIEYDIRD
                     KYSTRDGKQMPVPENLINA"
     gene            complement(5773..6486)
                     /locus_tag="PK288_05105"
     CDS             complement(5773..6486)
                     /locus_tag="PK288_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPM18332.1"
                     /translation="MRNYYDLLRQDVRFREGLNACMNCGVCTAICPAAEFYNYDPRQI
                     VDTVQSGDNSEIEDLLKSETIWYCGECMSCKTRCPRGNTPALVVMSLRTLSQQLGFFT
                     ESEKGRQQFAIKRSVGENIFKYGYCVATYAVSPEMHAEQGPIWKYILENTHDIYERLG
                     GQINELGAGPLRRIPDDAKEELRKIFDVTGGTELFNTIEEHSRKKASDLGLQFDDEGI
                     DNEYFIEVYTANNKNHTTP"
     gene            6905..7546
                     /locus_tag="PK288_05110"
     CDS             6905..7546
                     /locus_tag="PK288_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPM18333.1"
                     /translation="MNSYQFSATLIERFSRLKPLLDPIYKAAGIIIDCYSSGGKVLLC
                     GNGGSSSDADHTVGELMKSFEIKRPLDKSLRQRLEDLAGNRGKSLGTKLEHALPAISL
                     SSQHALNTAIANDIGFDMVFAQQIIGYGNPGDTLVAMSTSGNSPNVINACITAKALNM
                     SVIGLTGKKGGKMKPFCDILINVPETGTAAVQELHRPVLHILCRLTENHFYEK"
     gene            7558..9459
                     /locus_tag="PK288_05115"
     CDS             7558..9459
                     /locus_tag="PK288_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPM18334.1"
                     /translation="MKSEELNELLEKIRDVRIAVIGDFCLDAYWFIDKSTSEISLETG
                     RPTEPVRFQEYSLGGAGNIANNLAALGIRDVRALGVIGDDPFGTKMIRLLEQNGISTR
                     NMIVKADNWSTHVFIKPHVDEDEQNRIDFGNFNSLDNPTADQLISNLENELPELDLVV
                     INQQVFSGINTPYFRESLARLINRFPQKIFIADSRQYNDDYRGAWRKMNDIEALRLCG
                     IIRAFGENPSAGEVFSAAEKLHERYGKPLFITRGSRGSIVTDGKGIIESNGLMILTRI
                     DTVGAGDSYLAGISAAIAAGCSLEDAARLGTFVSGVTIRKLFTTGTASPAEIMETGRD
                     PDYIYNPDLAEDVRRAVYHGSHEIEIINPLPENTEIRYAIFDNDGTISTLREGWELIM
                     APMMIKAIMGEKYRDADETLYTKVTKAVSDLIDKTTGVQTLMQMKLLADLVRDFGLVP
                     ENQILDAHGYKKIFNDELLLMVRKREEKLNRQKLSASDLTIKGSLEFLRMLHGKGIKL
                     YLTSGTDVEDVKHEAGLLGYAGLFEGRIHGATGDIRHEAKKMVLDRLLDQIGESEAAS
                     IVTFGDGPVEIRETRKHGGITVGVASNELRRFGLNESKRTRLIKAGAGFIVPDFSSPS
                     ALLEILNIK"
     gene            9460..10587
                     /locus_tag="PK288_05120"
     CDS             9460..10587
                     /locus_tag="PK288_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18335.1"
                     /translation="MSYKRFDRDRLLVRKLSERKNQLSIKEIQVPASARPAALPAEAA
                     GLINLTARKIIAAREKGRSVMMAFGAHAIKNGLGTVLIRLIKEGWITHLATNGAGIIH
                     DWEIAYQGLTGESVRENVTTGQFGIWEETGKYINLAIIRGAAEKLGYGESVGKMISAE
                     GLEIPGREDMLTVISENIEKDPEHAAATLDLYNVVKKYNIAPGFLKIPHPFREYSVQC
                     SAYEQNVPFTGHPMIGHDIIYNHPFNCGAAIGRTALTDFLSYTWSVCNLNDGVYLSVG
                     SAVMSPMIFEKALAMCQNLEIQSGRHIDNHFMLIVDLAANEWDWNRKGEPPMDNPAYY
                     LRYCKTFNRMGGEMHYLQADNRDFLTELYHVLNNTPSSVLT"
     gene            complement(10580..12193)
                     /locus_tag="PK288_05125"
     CDS             complement(10580..12193)
                     /locus_tag="PK288_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPM18336.1"
                     /translation="MKLSLSGPDWIVLAIVMGGSLSYGLYMARRKKSGRDSSGFFLGG
                     RSIKWPVVGASLFATNIGAEHLVGLSGDSYRYGLSAGLVELTTVITLGIACAILFPYY
                     MKNKLFTIPEFLEIRYNRRARIFFSGLMLLISVMTKLAFTLFAGALVLHSILGWEIMP
                     TIFYIGIIVAVFTIIGGFTAVAYTDAIQVIVMIAGSALMLFFGLDRAGGWSGLMEKAP
                     EMMSIAKPYDDPVYPFWGILATAFYAGVFYWGMDQVNVQRVLAAPDLKNARWGAMFAT
                     LLKLLPVFIFALPGVIAFALFPGELQGDDTRQTFVLLLNKLLPAGLRGLLMASLLAAL
                     ITSLIGVLNSVSTLVVRDFIVEFKPGIPEKKQVLLGRYVIVAVTLLGFAAAYMVYRNE
                     EGLYKYLQTISAYLVVPVFPAILFGIMSRKVTYRGAAASVIVGVIIATIFVIDQLMGP
                     ETGKAVFPFLHHELTLNFGYRGLWAEIIITGVLFAVSAFTEKTDAERLERTTIDYSRG
                     LASFKGITDWRLHLGILTVITVAILLWLR"
     gene            complement(12195..13466)
                     /locus_tag="PK288_05130"
     CDS             complement(12195..13466)
                     /locus_tag="PK288_05130"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPM18337.1"
                     /translation="MEKQEIVRIREEVHDHLVNELLPFWTNRMTDVKNGGYVTHFDED
                     GTDTGEDEKSLIAQTRCLYTLSSAHRAGYGEGKLAKMAAQGLDFILDKMWDRQHEGFY
                     WMVDRKGNVKSDKKIIYGHSFAIYSLSEYTIATGDKRGIEYAEKVFDLVQKYCTDTMF
                     GGYWEMFERDWTLRGPGSAGGDRKTLDVHMHLMEAFTTLYECTGKEVHKRKLTEIIEI
                     LQNRINHPVYRTGIPQFFRNWTVAPQIKFEIIWGWDRFTGEGTKENVTDNTCFGHNAE
                     YAWLLNHALGILGADKTPYLKDFKTIFDHTLVNGIDTLYGGVFVEGPHSGGVSDREKE
                     FWQQAEVLIAMLDAFLLFGDQLYWNAYESVWRFVHDKMINRKTGEWYPLMTRDGVPVW
                     THMGHSWKINYHTVRSMILSLQKLDRIIKEM"
     gene            complement(13538..14440)
                     /locus_tag="PK288_05135"
     CDS             complement(13538..14440)
                     /locus_tag="PK288_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18338.1"
                     /translation="MKIINSLGTGFIRVVKAVKGIIITWFCFYFLVLTFVYPLRKSLD
                     FTFGSSMITERLADGFDFEVFADLGNALPSILSFFSTGFMITFFAGFLLNAFLTAGLF
                     GSVKKDSGRFSAPGFFSAGALNFWPFFLISLLITFGIIFLFIIIVLLPVSITAMAETL
                     PERSIFLVSLVSGIVFLLLIPVILLVADNSRSFKTGHREVPALKSIGKGFSLTSSDFG
                     RSYLMMLILILGQVVPGIAAILFLPAWKPVTGGGVFLLLVVSQLLFFARLFLKTWRYA
                     SVTARMDDILLTNTRGMEIQQVNN"
     gene            complement(14446..16413)
                     /locus_tag="PK288_05140"
     CDS             complement(14446..16413)
                     /locus_tag="PK288_05140"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HPM18339.1"
                     /translation="MKYKSGLLAGLISLAAVLQAQIINKTPLSERITGYTIRAVLDAD
                     AKTVSCTMEAFWVNRSADPVPDIQMHLYMNAFKSSRTTFYRERGGSPGSGELDRGWID
                     ITSFTDGNGNDLLSLMEFISPDDGNTEDRTVFRVILPEPAMPGDTVFIKTGFVTKLPS
                     RIIRTGFSGDFFFVAQWFPKFGVYEPAGMRFAASGGWNCHQFHANSEFYSNHSVYDVE
                     ITLPARFITGSGGLLMSETTAGDLKTVIYRAEDIVDFAWTAWPGYSVVNDRWRNVDIT
                     LMLPHDRIKQVDRQLTAVKNALGYLDENVGPYPWPHLTFVDPPAIGAGAGGMEYTTFF
                     TSSSSYFVPSFFYLPEMVTVHEFGHAYFMGILASNEFEEPWLDEGVNSYWEQRIMDHF
                     YGKNSGMINHRLFKLSDIAMGRMNYTRFPSRQASSNAEFSWNYPHGTYGIITYSKAAT
                     ILHTLEGIIGTETMNNVFREYYRKWAFRHPSGRDFINVVSEVVRKEHGLKFGPDMNWF
                     FDQTIYGTGVCDYKISDILNRRITKPEGPADLSDGASASVDTTGPQYTSIARLERVGE
                     LKLPVEVLIHFNNGDEIFETWDGKARYRDYEYQGSRKIEWVKIDPEYKNKMDVNLVNN
                     SMTTIPERTPLRRIAGRIASFTLFFLSIMLL"
     gene            complement(16550..16942)
                     /locus_tag="PK288_05145"
     CDS             complement(16550..16942)
                     /locus_tag="PK288_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18340.1"
                     /translation="MKARYICFFTVLLFFIITASGQDGTAKKHNPAGSWKFEAPSAPP
                     GYNSGTITVSLAEKKYSLTVLPQGSSYKITGEGVKVENDQLTCSVYVEGEYVRVTLKA
                     EDKNKMTGTASYSGGLIPLTLTRMQETR"
     gene            complement(16965..17564)
                     /locus_tag="PK288_05150"
     CDS             complement(16965..17564)
                     /locus_tag="PK288_05150"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPM18341.1"
                     /translation="MISSISGVILAGGASRRFGGIQKAKLLAGGTTIISRIISAIGEL
                     FPEIIIVTNTPGGFEDLQGCIVTRDHFLNAGPLAGIHAAIRASSCSAVFVFAGDMPLI
                     DRDIVKRQAEAFFESDYEILIPRRGDLTEPLHSVFRVSILHDLEKYLTEKHDRSVRDF
                     IAGRHTGFFELEDTPATKRAFTNINSPSDFRKFRTDYYL"
     gene            complement(17603..18229)
                     /locus_tag="PK288_05155"
     CDS             complement(17603..18229)
                     /locus_tag="PK288_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPM18342.1"
                     /translation="MKPRNLLALILIPMGLIIAAVPQNKTNPYKLTADQMLTEANSRT
                     QFVAPEVVADMILRKDPSLRLIDVRGQDEFEKFSLPGAINIPSSDLLSDEYTDILNQD
                     IKMNVFFSNGTLAANQAWMITRQLGYENNYVLEGGLNYWFEAILNPQKPVSTSSDEEF
                     ARYDFRKSLGQALGGGGDAVQPASGQDAFTVKPVIKPAAKKKRAAGGC"
     gene            complement(18267..18857)
                     /locus_tag="PK288_05160"
     CDS             complement(18267..18857)
                     /locus_tag="PK288_05160"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPM18343.1"
                     /translation="MGPLAPNEIISEDTNFLLAFFIGIAFGWVLESSGFSSSRKLAGV
                     FYGYDAVVLKVFFTAAITAMLGLLFMSLFGWIDLNLVYINPTYLTSAIVGGVVMGAGF
                     IMGGFCPGTSFCGAAIGKIDAMVFIGGLFIGIYGFAFTYGWWEKLYLAKYLGEPKVSE
                     VLGLSDGVFGLLLILVALAMFAVAEWAEKKFPREAY"
     gene            complement(19036..19569)
                     /locus_tag="PK288_05165"
     CDS             complement(19036..19569)
                     /locus_tag="PK288_05165"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPM18344.1"
                     /translation="MTTTKETRANRYLNPYLGGVLLGLVLLSANFISGRGLGASGAVK
                     SVIAFTIEKADRNAAGKMPFLNEYAESHSGNPLKTWLVFQMLGVVVGGFLSGALAGRL
                     KLKVEHSPGITSRRRLIFALAGGILFGFGSQLGRGCTSGSALSGMAVLSLGGFVTMMA
                     IFGTAFMLAYFFRKNWI"
     gene            complement(19602..>20370)
                     /gene="nrfD"
                     /locus_tag="PK288_05170"
     CDS             complement(19602..>20370)
                     /gene="nrfD"
                     /locus_tag="PK288_05170"
                     /inference="COORDINATES: protein motif:HMM:NF025940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPM18345.1"
                     /translation="VRVAPFLAPILLVIGLLALFADLRHRMYFWQLYANIRMQSPMSW
                     GAWTLMVITPVSFIWSALHIRDIFPEWNWRYKWLFNLEVFFNRHKTALAWIMLIFGLI
                     LGIYTGILLSAFNARPLWNTSILGPLFLASGLSAGAATIIFLSKDPAERKQFAKIDLA
                     IIGIELFLIIHMFMGYLASSQVQIEAARMFLGGEYTASFWIFVVILGLIIPALLEILE
                     LRKYHIPAVVSPLLVIFGSFMLRFIIVYAGQMSRYLY"
BASE COUNT         5315 a         5091 c         4812 g         5152 t
ORIGIN      
        1 atcatacgcg agaagggagt gaacggcgac agggagatgg cttatgccct ctggctcgca
       61 ggtttcgatg tcagggatgt gcatatgact gatctgatga cgggccggga gactctcgac
      121 gatctgaaca tgatcgtttt tgccggggga ttctccaatt ccgatgttct tggatcagca
      181 aagggctggg ccggtgcatt caggtacaat gaagcagcag ggaaggcgct ttcaacattc
      241 ttcagcaggc ctgacaccct ctcgctcggc gtctgcaacg gctgtcagct aatgaccgaa
      301 ctaggcctca tttatcctgg gcatgaacaa aggccggagc tgcttccaaa cagttccggt
      361 aaattcgagt cgggatttat ctcagtgaag atcccggaaa acaggtcggt catgctggga
      421 aatatgtcgg gcatggaact cggcgtctgg gttgcacacg gggagggaag gttcaatttg
      481 ccttatcccg aagaaaaata tcatattgca ctcaaataca gcagcagcac ctatcctgcc
      541 aatcctaacg gttccgcata tgatgtggcg ggactgtgtt cagatgacgg aaggcatctt
      601 ataatgatgc cgcatccgga gagagccttc tttccgtggc agtgtccgta ttatccgaat
      661 gaaagaaaac aggatgatgt gacaccctgg ataaaggcat tcataaacgc caggaagtgg
      721 atcgaaaaaa cgctcagcgg gtcataggtg tcagaagcag tgacatcagc cgggtgtctt
      781 cagtgaagcc tgaatgctgg ttcatggctt cacggggaag aactttctcg cttatcacct
      841 gcaaattgta agctcccgcc tccagaacat aaaatgaccc caggtaggaa tcagccctgt
      901 aatagggata ggatatatcc tccgaatttt ttacaacctt atcacaaaca gggtcaacct
      961 caaggaaact gtcaagaaaa gtcaccttga ccgagttcac ataactgaga tcggttgtgt
     1021 ccctggtcgc gcttgtaagc cagaccttga aacgtccggg ttttgcaata acaactttcc
     1081 attcagcggt gttgcttgca ggattggata tgtcattata acaggcagcc ttgtcaatgt
     1141 tgagaacaag ggagccgtct tcctgctgga ttacggtatt tccttcattg gcctgatcaa
     1201 ttgatcggga tttatttcca cacgaccaca gaaacatgca accaatagca acagaaacga
     1261 taattctgct tttcattttt tccccttatt acgattccaa ctaataccgt ttaattcaga
     1321 tttcaactcg gcaaaattaa ataaaaaaca aataacgtaa aattcattta gtttttgact
     1381 aaatgcaatt acttcttttc aacagttgtc agattattat tgttcaaaaa ccgtgctgaa
     1441 atctggatgg ttcatgcatt ttaacatatc cgggtatgga ttttttttca acaggttcgt
     1501 taataagcgc catttcagcc agttcggcaa tatcaactac ttttgttgaa gttgcatttg
     1561 ccagagtttt tttgcacagg gggcaggaag tgacaagaaa atcgggatca gatgctgtaa
     1621 ggattttagc ggcatcggca gcaatgcttt tccttttttc ctggctgagc attatgtttc
     1681 cgaggcttcc tccgcagcaa agtgcattct catcatcata accggtcatt tcaagcctgg
     1741 ccagatgcat cagcacctgc ttcggttcct catatacgcc ggaatttctt ccaaggtcgc
     1801 agggtgtatg gtatgttacg cgattatgaa ggaagttcat tcttaccagg tcttcatcaa
     1861 taattttact gatgaactgg gaatggtgca tcacttctgc atccagatag taactttccc
     1921 tgaaaacctt gaagcatatc gggcatgagg tgacaaggat atctgccccg gcatcgagga
     1981 ttatctgcga attgtaatta ataagttccc tggcttccct gtcctgaccg gccagcatca
     2041 gggggcgtcc gcaacaggcg ccggcatgct cgtccataaa cagccattcc agtccgctgg
     2101 catcaaggat cctggtcatt gcatttttta tcgcaggggt aagatgcgtc atgcatcctg
     2161 caaaatacaa tacacttgct tttgccttgc ttccggacgg aaggtagtca tatgaaggtg
     2221 tactgacggg tctgggccgg gtattttttt tccggtcggg gaaataggcc ttaaaaaggc
     2281 tttgccgttc cttaaccgga tctcctgccc tcctctgtat cattcttaca ggcgacaact
     2341 cgatacctac aggacattta acatcgcagc gtccgcacaa aaggcaattg aaggcagtat
     2401 aatttgtttt atcattattc cggatggcct tcagcaggta aactgactgc atatcgttga
     2461 ttcctgccga gaagctcatc tggcacgcgt cgatgcatat cccgcaggaa gagcatgaat
     2521 gcacttccac gtcagagaat gttcctgcct tgtcgcctgt ccttatccct gaatttctca
     2581 tgaagataag aaagagctcc gtgggaatat gcatgtaccg ggtaagcggg agcagtataa
     2641 agaatgttcc gagagacagg gagtacagcc accagaaggg gtatgccagt tcactggcag
     2701 gaaggaagga tgcaagttga gcccccaggg taccggtcag aaagcttccc gttccatatg
     2761 cgccgcttgt caggctttcg gcaataagac ggctcggaaa aatgagccat aatgaagcca
     2821 gtgccacctt gtcgggcagg cggtgcctgg tggttttttt catcccgacc gtacccggcc
     2881 agaacctctt gaatattgca agaagcagac ccgacaggat aaaggcaaga atgaggtcca
     2941 tcaggaacgc atagaatgat tcaaaaggtg tttttccgtg agcgggattg aagaacctga
     3001 aaaagatcgc tttgtagggc ggattgaggt gcttttgacc gaaaatgtcg gcttcaatgg
     3061 taccgaacag tatgagcagg aaccagccga aggccaggct catgtgcata tatccgaggc
     3121 ggaaattcgt tttgaggatc ttcctgtgta tgaggctttc catgaagatt tccctcaggc
     3181 tttgcccgaa tgcccttccg aagaatcctc tctgcaatct caacttgtcg gggcgtgaca
     3241 gatcacggta ccagatcaca cttctcgcca ccgcatacag caggatgaag tacaatccta
     3301 tattgaaggg tattataaaa ggttcaagtt tcatttccgt caaagatcct gaaagcctgt
     3361 cgtgcaagga aaataacatt cctggtgttt acaccgcgag ggcaggcaag aatgcatttg
     3421 ccgcaaaaca tgcatttttt cagcctggcc cttaccttgt cattctcacc gcgctttaaa
     3481 agtacattga actccctgag gctgaattct gtaaaattgc cagccgtaca tgttgcggcg
     3541 cacgcaccgc attcaatgca aagcctgaag ctcggttcgt gctcatatat atattcagcg
     3601 atcctcctgt cgtttttatc gtaatcgata acccttgccc gtgacaatga aaatccgaat
     3661 ttatccatta tgtctccctg tattcctgag gtaatcgatt atctcagcag ctgctcccct
     3721 tgcgtgcgag atcgtgtccg tcacattcat cggtgcggtg caggtgccag catagaaaat
     3781 tccgcttata tggctcctgt tgctcccgta atggttgtcg gcagatgcaa ggaacctgtt
     3841 ttcccctgtt ttaagtcccg atgcaagagc cagctttttg cctccgtccg atatctccat
     3901 gcctgccatg agtaccagca tgtcgacttc cattttcagc ggtcttccgg caagtgtatc
     3961 ttccgacttt acaataagcc tgttatcaat actgacactt acctctgaca cctttcccct
     4021 gataaagttt ataccgtatt tctcctgtgc ctcacggtag agctcttcat acatggcccc
     4081 tcccattctc atgtccatgt aaaagcagaa tattctggca tcactgatat attcctttat
     4141 ttccatggcc tgctttacgg ctgtgacgca gcacagcttg gagcaataaa ggtttccgac
     4201 tttttcgtcg cgtgaaccca cgcagtggat cattcctaat tttgacggaa ctattccgcc
     4261 gaccgttttt atctctccgt tcctgaacat gacctcaagc tcggcagagg ttataacatt
     4321 gtcatagatc ccgtaaccgt actcctcttt tctctcacac cggaacatat caaaccccgt
     4381 ggcaataaga acagcatctg cagtgaatga tccacccttt ccggttgtca cactgaagga
     4441 taggcctttc ctgtcgatct ttgcaacggt ttctccggga ataaaatcaa tgttctcgtg
     4501 actgataagg cggctgagat agctgatgac ttccctgctg tcgcgacggt cggggaacag
     4561 gtgataccat cctttcagat ggcccccggg ttctgtttcc ttttcaatga gtgtcacccg
     4621 gatccccgat cttgcaagca ttcccgcagc ctcaatcccc gccgctccgc cgcctgcaac
     4681 aacaacgtgg gcactcattt caggcattga taaggttttc aggaacgggc atctgtttgc
     4741 cgtccctggt cgaatattta tcgcggatat cgtattctat tcccattttt ctcagcagag
     4801 gttccgactg caccatgtga tactggagac ccaggtccca gggattgtat cccagaacca
     4861 gccccgcaag ctcctcgtat gtcagcacgg gaatggattc cccgttgaca ccgaaggttg
     4921 tgccgtccat ttcctttatg gtgtattgcc atttgtccat gaacatggtg catccgggac
     4981 agttagtaag aaccaggtcg ggcttgaaag gtttcatcga ggaaaacttc tttgcggtat
     5041 tggaaaggga ataaccccgg tttgcctgta caagatattg cctaaatccg aatccgcagc
     5101 aatggcgtcg ttccgggtag tcaaccacct caccgcccca ggcttcaatc atcccgctga
     5161 gaactttcgg gaattcggcg cctcccaccc cgtacttggg gaatatcttt gcataatggc
     5221 agccaatatg ttccacgact ttcaggggtt cacccgtctg cctgttcaca aggagatatt
     5281 ttgcatttgc cgcaatctca ttcctgaatt tgtaaatgat atcgcttgta tgggctatgt
     5341 tctccggcag atcaaactcc ctgccggttg ctttataaag atattccctg gttttttcaa
     5401 gctcctccgg aaaatgtttc caggtttcaa gtatctcggc atatattcca aatgatgtca
     5461 cgcatgaaat ggcgatgttt ttgtaaccgg cctccttcat gagtgcatag tgccgtgcta
     5521 tgaccgtcat aatcgtctga aagggcacaa tgtcggcatg atatccgatc ccggtgcagg
     5581 ttgtgtggga aggattctca aagacatcct ttccaagctt gtcgcgcagg aagtcgagaa
     5641 aaaacttctc cgatcccggg aagaaactct gcctgatgca tgacctgacg tagaaaaagt
     5701 ggtcatccgc tatctctttc tgatagtcag accagatcct ttcactgccc tcgctctcca
     5761 ttactgtcag aattatgggg ttgtatgatt tttattattt gccgtgtaaa cctctatgaa
     5821 atactcatta tcgatccctt catcgtcgaa ctgcaagccc aggtctgagg cttttttcct
     5881 ggaatgttcc tctatggtgt tgaataattc cgtcccgccc gtaacatcaa agatctttct
     5941 cagttcttcc ttagcatcgt ccgggattct cctcagcggt ccggctccca gctcattgat
     6001 ctggccgcca agccgctcat aaatgtcatg ggtgttttcc aggatgtact tccatatagg
     6061 accctgttcg gcatgcattt cagggctgac cgcgtaagtt gccacacagt agccgtactt
     6121 gaaaatattc tctcccacac ttctcttgat tgcaaactgc tggcgccctt tctcagattc
     6181 ggtgaagaag ccaagctgct gtgacagggt ccggagcgac atcaccacca gggccggtgt
     6241 gttacccctc gggcaacgtg tcttgcacga catgcactca ccacagtacc atatggtctc
     6301 cgatttcagg agatcctcta tttcactgtt gtcgcccgac tgaacagtat cgacaatctg
     6361 ccgcggatca tagttataga attcggcagc agggcatata gcagtgcaga caccgcagtt
     6421 catgcaggca ttaaggccct ccctgaacct gacgtcctgc cggagaagat cgtaatagtt
     6481 tctcatcccc tgatgatgaa ggatctggct tctgttaatt tattaacaca aaggtatttt
     6541 tttcaacagc gtgatgaaag aggcttaaaa cccattttaa aatgagtatt tatacttagg
     6601 ccgggaaaac ggatattgcc ccttgaggct cagaagcatg gcacttcagg gaaatccggc
     6661 aggaagcccc catctgatcc ggaatatcag gaaaactgct tcttctgcat ggcttcgcga
     6721 aaaaggcttc ctatgcatac gggaaacagc acgaggcaaa atgacccgga ggcttttata
     6781 atgtcaatga tttattcaca tcgtccggca gataccggtc attcaatttt tcccggtcat
     6841 tctcctttca aacaggaaac ccgcgaaaaa gaagtaaatt tatacatcga ttaaaattat
     6901 tataatgaac agctatcaat tttcagcaac gcttattgag cggttttcaa ggctaaagcc
     6961 ccttcttgac ccgatataca aggcggccgg catcatcatc gattgctatt catcaggagg
     7021 aaaggtatta ttatgcggaa atgggggcag cagttccgac gctgaccaca cggtagggga
     7081 actgatgaaa agtttcgaga taaaaagacc tcttgacaaa agtctcaggc aaagactgga
     7141 ggatctggca ggcaacaggg gcaaatcact gggaacaaag cttgaacatg ctctcccggc
     7201 aatcagcctc tcatcccaac acgctctgaa cacggcaatc gcaaatgata tcggctttga
     7261 tatggttttt gctcaacaga taatcggtta tggcaacccg ggcgatacac tggtggcgat
     7321 gagcacgtcg ggtaattcgc caaacgtgat aaatgcgtgt atcacagcca aagcattgaa
     7381 catgagtgtt atcgggttaa caggcaaaaa gggcggaaaa atgaagccct tctgtgacat
     7441 tttaatcaac gtgccggaaa ccggtacggc agctgttcag gagctgcatc gtcccgtgct
     7501 gcatatcctg tgccgcctga cagagaacca tttctacgaa aaataaaaaa ttgatacatg
     7561 aagtctgaag aactaaatga gctcctggaa aaaatacgtg atgtcaggat cgccgttata
     7621 ggtgatttct gcctcgacgc ctactggttt attgataaat caacaagtga aatttctctc
     7681 gagaccggca ggccaacaga gcccgtcagg tttcaggaat attcccttgg cggtgccgga
     7741 aatattgcca ataacctggc ggccctcggt ataagagatg tgagagccct gggcgttatc
     7801 ggtgatgacc ccttcggtac gaagatgatc aggctgctgg aacaaaacgg gatcagcacc
     7861 aggaacatga tcgttaaggc cgataactgg tcaacccatg tttttatcaa accccacgtt
     7921 gatgaagatg aacagaacag gattgatttc ggcaacttta acagcctgga taatccgact
     7981 gcggaccagc tgatcagcaa ccttgaaaac gaacttccgg agcttgacct cgtggtgatc
     8041 aatcaacagg ttttttcggg cataaatacc ccgtatttca gggaaagtct cgccaggctt
     8101 ataaaccgtt tcccgcagaa gatctttatt gccgacagcc gtcaatacaa cgatgattac
     8161 cggggagcct ggagaaagat gaacgacatc gaggcactga gattatgcgg cataatcagg
     8221 gcgtttggcg agaatccttc agccggggag gttttcagtg ctgctgaaaa gcttcacgag
     8281 cgttacggga aacccctgtt catcacgcgc ggcagccgcg gttccatagt cactgacgga
     8341 aaaggtatta tagaatcaaa cgggttgatg atactgaccc gtatcgacac ggtcggcgcg
     8401 ggcgacagtt atcttgcggg aatctccgcc gccattgctg caggctgttc actcgaggat
     8461 gctgccaggc tgggaacatt tgtctcgggt gttacaatca ggaagctttt tacaaccgga
     8521 actgcttcgc cggcggagat aatggaaacc ggaagggatc ctgattacat ctacaatccc
     8581 gaccttgcag aggatgttag acgtgccgta tatcacggaa gccatgaaat agaaataata
     8641 aacccactgc cggaaaacac ggaaatcagg tacgccatct tcgataatga cgggacaatc
     8701 tcaaccctca gggaaggatg ggaactgata atggcgccca tgatgattaa ggccatcatg
     8761 ggtgaaaaat acagggacgc tgacgaaacg ctttacacga aagtaacaaa agcagttagc
     8821 gatctgattg acaagacaac cggtgtgcag accctgatgc aaatgaagct gctggcagac
     8881 ctggtccggg attttggact tgttccggaa aatcagattc ttgacgcaca cggttacaag
     8941 aaaatattta acgatgaact gttactcatg gtaaggaaac gggaagagaa actgaacagg
     9001 cagaaactca gtgccagcga tcttaccatc aagggttcgc ttgaattcct tcgaatgctt
     9061 cacgggaaag ggatcaaact atacctgacg agcggcaccg acgtggaaga tgtgaagcac
     9121 gaagcgggat tgcttggata cgccggcctt tttgaaggac gaattcatgg agccaccgga
     9181 gatatcagac atgaggcaaa aaaaatggtt ctcgacaggt tgcttgatca gatcggtgaa
     9241 tcagaggccg cttcgatcgt gactttcggc gatggcccgg tggagatcag ggaaacacgc
     9301 aagcacgggg ggatcaccgt gggggtggca agcaacgagc tccgcaggtt tgggttaaat
     9361 gaatcaaaaa gaacaaggct catcaaggca ggagcaggtt ttatcgtacc ggatttctct
     9421 tcaccgtctg ccctgctgga aattttaaat ataaagtaaa tgtcatacaa gcgatttgac
     9481 agggaccgcc tcctggtccg gaaactttct gagcgaaaaa accagctttc aataaaggag
     9541 atacaggttc cggcatccgc ccgtccggct gctctcccgg ctgaagccgc tggcctgata
     9601 aaccttacgg cccgaaagat tatagcggca agggaaaaag gcaggtcggt gatgatggct
     9661 ttcggtgccc atgcaataaa aaatgggctg gggacggttc ttatccgttt gataaaggaa
     9721 ggatggataa cccatctggc aacaaatggt gccgggatta ttcatgactg ggaaatcgcc
     9781 taccagggcc tcacgggtga aagtgtcagg gaaaacgtga caaccgggca gttcgggatc
     9841 tgggaagaaa ccgggaaata cataaacctt gccattatca ggggtgctgc cgaaaagctc
     9901 ggctacgggg aatcggtcgg gaaaatgatc tccgccgaag gacttgaaat tcccggccgg
     9961 gaagatatgc tgaccgtgat aagtgaaaat attgagaagg atccggaaca cgcggccgcc
    10021 acgctggatc tgtacaacgt ggtgaaaaaa tacaacatcg caccgggttt cctgaaaatc
    10081 ccgcacccgt tcagggaata ctcggtccag tgttcagcat acgagcaaaa cgttccgttt
    10141 acgggccacc cgatgatcgg gcatgacatt atttacaatc atccctttaa ctgcggggca
    10201 gcaatcggca ggacggcgct tacagacttc ctttcctata cctggagcgt ttgcaatctt
    10261 aatgacggtg tatatctgtc tgtaggatca gcagtaatgt cacccatgat atttgaaaaa
    10321 gctctcgcca tgtgccagaa tcttgaaatt cagagcggca ggcatattga caatcatttc
    10381 atgctgattg ttgatcttgc cgccaatgaa tgggactgga acaggaaagg agagcctcca
    10441 atggacaacc ccgcctacta tctcaggtac tgcaagactt ttaacaggat ggggggcgaa
    10501 atgcattatc tccaggctga caacagggac tttctcactg aactgtatca cgtcctgaat
    10561 aacactccct cttccgtcct cacctgagcc acagcagtat tgcaaccgtg ataacggtaa
    10621 gaatgcccag gtgaagccgc cagtcggtaa tgcctttaaa ggaagcaagt cccctggagt
    10681 aatctatggt ggttcgttcc agtctttcgg catctgtttt ttccgtgaaa gctgaaacgg
    10741 caaacaggac accagttata attatctcgg cccacaatcc ccggtacccg aaattcaagg
    10801 tcagttcatg atggagaaaa gggaaaactg ctttgcccgt ttccggtccc atcagctggt
    10861 cgataacaaa aatcgtggca atgatcactc ccacaataac cgatgccgcg gcgcccctgt
    10921 aggtcacttt cctgctcatt ataccgaaca ggatggcggg aaatacaggc acgaccaggt
    10981 aggccgaaat cgtctggagg tacttgtaca ggccctcttc attcctgtaa accatgtaag
    11041 cggctgcaaa acccagcagt gtaacagcca cgatgacata ccttcccagg agaacctgtt
    11101 ttttctcggg gatcccgggt ttgaactcga ctatgaagtc ccgcaccacc agtgttgaaa
    11161 ctgaattaag gacaccaatc agcgaggtta tcagggccgc cagcagtgat gccatcagta
    11221 gtccccgaag gccggcaggg agaagcttgt taagcaggag aacgaaagtc tgccttgtat
    11281 catcgccctg cagttctccc gggaaaagcg cgaaagcgat aaccccgggg agggcaaata
    11341 taaaaacggg aagaagcttc agcaatgtag caaacattgc cccccagcgc gcattcttta
    11401 gatccggtgc cgccaggacc cgctgcacgt taacctggtc cattccccag taaaaaacgc
    11461 ctgcgtaaaa tgctgttgcc agtatacccc agaatggata aaccgggtca tcatagggtt
    11521 ttgctatcga catcatctcc ggggccttct ccatcaggcc cgaccacccc cctgccctgt
    11581 ctaggccaaa aaataacatt aaagccgaac cggctatcat cacgatcacc tgaattgcat
    11641 cggtatatgc cactgccgtg aatccgccaa ttatggtaaa aacagcaacg atgatcccga
    11701 tgtaaaatat cgtgggcatg atttcccagc ccaggatgct gtgcaggacg agggctcccg
    11761 cgaacagggt gaaagccagc tttgtcatca cgctgatcag cagcatcagt cctgagaaga
    11821 aaatccgggc ccgacggtta tacctgatct cgagaaattc agggatggta aaaagcttgt
    11881 tcttcatgta atacgggaac agtatggcac aggcaatccc gagtgttatg accgtggtaa
    11941 gttccaccag ccccgcagaa agcccatagc ggtaactatc acccgaaagc cctaccaggt
    12001 gttccgcccc gatgtttgtc gcgaataatg aagcccctac aacgggccac tttatggatc
    12061 tccccccaag gaagaaaccg gaactgtcgc ggcctgactt tttcctccgc gccatgtaca
    12121 gcccgtaact caggctgccg cccattacga ttgccagtac aatccagtca ggacctgaaa
    12181 gggacagctt catcctacat ctctttaatg atcctgtcca atttttgtaa ggagagtatc
    12241 atcgacctca ctgtatggta attgattttc caggaatgcc ccatgtgtgt ccagacaggc
    12301 accccgtcgc gtgtcatgag cgggtaccat tccccggttt tcctgttaat cattttgtca
    12361 tgaacgaaac gccagacact ctcataggcg ttccagtaca actgatcccc gaatagcagg
    12421 aaagcgtcga gcatggcgat caggacctct gcctgctgcc agaattcttt ttcacggtca
    12481 gaaacacctc cactgtgcgg tccttcgaca aaaacaccgc catacagggt gtcaataccg
    12541 ttgaccaggg tatgatcgaa aatcgtttta aaatctttga gataaggagt tttatcagct
    12601 ccaagaattc cgagggcatg attcagaagc caggcgtatt cagcgttatg cccgaaacac
    12661 gtgttgtcag tgacattttc ttttgtaccc tcgccggtaa acctgtccca tccccatatt
    12721 atttcaaatt taatctgcgg ggcaacggtc cagttcctga aaaactgggg tatgcccgtc
    12781 ctgtatacgg ggtggtttat cctgttttgc agtatctcaa ttatctcggt aagttttctt
    12841 ttatgaacct ccttccccgt gcactcgtaa agcgttgtaa acgcctccat caggtgcatg
    12901 tgaacgtcaa gggtcttgcg gtctcccccg gcacttccgg gacctctcaa agtccagtca
    12961 cgttcaaaca tttcccagta gcccccgaac attgtatctg tacagtattt ctgtacaagg
    13021 tcgaaaacct tctcagcata ttcaatacct cttttatcac ccgtggcaat cgtgtattca
    13081 ctcaacgagt agattgcaaa gctatgcccg taaataatct tcttatccga tttaacgttc
    13141 cctttcctgt caaccatcca gtaaaagcct tcatgttgcc tgtcccacat tttatcgaga
    13201 ataaaatcca ggccttgggc tgccattttt gccagttttc cttccccata cccggccctg
    13261 tgtgccgatg acagggtata gaggcatctt gtctgtgcta tcagtgattt ctcatcctca
    13321 ccggtatcag taccatcttc gtcaaaatga gtgacatatc ccccattttt tacatctgtc
    13381 attctatttg tccagaaagg aagcagctcg ttcacgagat gatcatgaac ctcttccctg
    13441 atccggacaa tctcttgttt ttccattttt tcaggatata ggtgtggaaa ggatttcagc
    13501 ctgagatccg aatcttaaat accagtttaa aaataattca gttatttacc tgctgtattt
    13561 ccattccccg tgtgttcgtc aataaaatat catccatccg ggcagtaaca ctggcgtaac
    13621 gccatgtttt caggaaaagc ctggcgaaga aaagcagttg tgaaacaact aacagtaaaa
    13681 atactccccc gcctgtcaca ggtttccagg ccgggagaaa gagaatagca gctattcccg
    13741 gaactacctg gcccaggatc aggatcagca tcattaaata ggatctcccg aaatctgagg
    13801 atgtcaggga aaaacctttc ccgatagact taagggcagg gacttcacga tgacccgttt
    13861 taaaagacct ggaattgtcc gctaccagta atataacggg tataaggagc aggaacacga
    13921 tcccggaaac cagtgatacc aggaaaatac ttctctctgg cagggtttct gccattgcag
    13981 taatactcac cggcaacaat acaataatga taaacaggaa aataattcca aatgtaatca
    14041 gcagtgatat cagaaaaaaa ggccagaagt taagggctcc cgcgctgaaa aacccggggg
    14101 cggagaacct gcctgaatct ttcttcacgc tgccgaacag gcctgccgtg agaaaggcgt
    14161 tgaggaggaa tcctgcaaaa aaggtgatca tgaatccggt tgaaaagaat gacagaatgg
    14221 aaggcagagc atttcccaga tctgcaaaaa cctcgaaatc aaaaccgtcg gccagtctct
    14281 ccgttatcat gctcgagcca aaggtgaaat caagtgactt tcttaaagga taaacaaaag
    14341 tcaggaccag gaaatagaaa caaaaccagg ttatgataat gcccttaact gctttcacga
    14401 cccttataaa ccctgtccca agggaattga ttattttcat tttgcctaca ataacatgat
    14461 gcttagaaaa aatagcgtga aagaggctat ccgtcccgcg atcctgcgga gcggagtcct
    14521 ttccgggatc gtggtcattg aattatttac caggttcacg tccattttgt ttttatattc
    14581 cgggtctatt ttcacccatt ctatttttct gctgccttga tattcgtagt cccggtacct
    14641 ggctttcccg tcccacgttt caaatatctc gtcgccattg ttaaaatgaa tcagtacttc
    14701 aaccgggagc ttaagctctc cgactctctc cagcctggct atggaggtgt attgcgggcc
    14761 ggttgtatca acacttgctg aggcgccatc tgaaaggtct gccggtcctt ccggttttgt
    14821 aatcctcctg tttaaaatat cgcttatctt gtaatcgcag actccggtcc cgtaaatagt
    14881 ctggtcaaaa aaccagttca tgtccgggcc aaatttaagt ccgtgttctt ttctcaccac
    14941 ttcgctcacc acgtttataa agtccctgcc ggaaggatgc ctgaaggccc atttcctgta
    15001 atattcccgg aacacgttat tcatggtttc agttccgatt attccctcga gggtgtgcag
    15061 aatggttgca gctttggaat aggttattat gccatacgtt ccgtggggat aattccagga
    15121 aaattcagca ttgcttgagg cctgtcttga tggaaatctc gtgtaattca tcctccccat
    15181 tgcaatatca gataatttga aaagcctatg gttgatcata cccgagttct tcccgtaaaa
    15241 atgatccatt attctctgtt cccagtacga attaactcct tcatcgagcc acggttcctc
    15301 gaactcgttg ctggccagaa tacccatgaa ataggcatgt ccgaattcat gaaccgtgac
    15361 catttcaggc aggtaaaaga aggagggcac gaagtatgat gatgaagacg tgaaaaatgt
    15421 cgtgtactcc atccctcctg ccccggcccc tatggctgga ggatcgacaa atgtaagatg
    15481 cggccacggg tatggcccga cattctcatc aagatacccg agcgcgtttt tcacggccgt
    15541 gagctggcgg tcgacctgtt taatcctgtc gtggggaagc atcagcgtga tatccacgtt
    15601 tctccaacga tcatttacca cggaataacc cggccaggct gtccaggcaa agtcaactat
    15661 gtcttcggcc cggtatataa ccgttttcag gtcacctgcc gtagtctcag acatgagcaa
    15721 gccgcctgaa cctgtaataa aacgggccgg cagggttatt tccacatcgt aaacgctgtg
    15781 attggaatag aactcggaat ttgcatggaa ctggtggcaa ttccagcctc ctgatgcagc
    15841 aaaacgcatc cctgccggtt cataaacccc gaacttggga aaccattgtg ccacgaaaaa
    15901 gaagtcgccg ctgaatccgg tccttataat cctggaaggc agcttggtaa caaaaccggt
    15961 tttgatgaaa accgtgtcgc caggcatggc cggttcagga aggataaccc tgaatacggt
    16021 cctgtcctct gtgttcccgt catcgggact gataaactcc atcagggaaa gcaaatcatt
    16081 accgtttcca tcggtgaagg aagtgatatc aatccatccc cgatccagtt ccccgctgcc
    16141 gggcgaacct ccccgctcac ggtaaaaggt tgtccggctg cttttgaaag cgttcatgta
    16201 aagatgcatc tgaatatccg gaacaggatc agcggacctg ttgacccaga aagcttccat
    16261 cgtgcagctg acggtctttg cgtcagcatc gagtaccgcc ctgatggtgt aaccggtgat
    16321 gcgttcactc agaggcgttt tatttattat ctgagcctga aggacggctg ccagtgaaat
    16381 caacccggcc agcaggcctg acttatattt catacggttc acatagtgat tggaaacata
    16441 aaacaacatt tatcctcccg tactccggta atttccctga tgtgccggga agggcctgta
    16501 ttccccggtc gggccggctg ccgcataaaa acaggaagtt ccggaaagtc tatctggtct
    16561 cctgcatcct tgtaagggtc agtgggatta atcctccgga ataactggct gttccggtca
    16621 tcttgttttt atcttccgct ttcagggtga ccctgacata ttctccctca acataaacag
    16681 aacaggtgag ctggtcattt tccaccttca ccccttcgcc ggtgatctta taactgcttc
    16741 cctggggaag gacagtgaga gagtactttt tttcagcgag gctgacggta attgttccgg
    16801 aattgtatcc gggaggtgcg gagggagcct caaacttcca gcttccggcc ggattgtgtt
    16861 tttttgcagt tccgtcctgc cccgatgcag tgatgatgaa aaacagcaat acggtgaaaa
    16921 aacagatata ccgtgctttc atagtgataa ttgattggtt tatttcaaag ataataatct
    16981 gttctgaact ttcgaaaatc ggaaggggaa ttaatgtttg taaaggctcg tttcgttgcc
    17041 ggtgtatctt ccagttcaaa aaagccggta tgtctcccgg caatgaaatc ccttacggat
    17101 ctgtcatgct tctctgtaag gtatttctcc agatcatgca ggatcgaaac cctgaaaaca
    17161 gagtgcaaag gttcggtgag atctcccctt ctgggtatga gaatctcata atccgattca
    17221 aaaaaagcct ctgcctgccg tttgacaata tccctgtcaa tcaggggcat gtcaccggca
    17281 aaaacaaaaa ccgcactgca ggaggatgcc ctgatggctg cgtgaattcc ggcaaggggg
    17341 cctgcattca ggaaatgatc gcgggttacg atacagccct gaagatcctc aaaaccgccc
    17401 ggtgtatttg taacaatgat tatttccgga aacagttccc cgatagctga gattatcctg
    17461 gatattattg ttgttccccc ggccagcagc ttcgcctttt gtattccccc gaagcgccgg
    17521 ctggctcctc cggcaaggat cacgcccgaa atactgctaa tcattctgat cgggttaaag
    17581 ggctgtttgc ggggcagcaa tatcagcagc cgcctgcggc cctctttttc tttgctgccg
    17641 gttttatgac cggcttaact gtaaatgcat cctgcccgga ggctggctga acagcatcgc
    17701 ctccgccccc gagggcttgt ccgagactct tccggaaatc atacctggca aattcctcgt
    17761 cggaactggt ggaaaccggc ttctgcgggt tcagaattgc ctcaaaccag taattaagtc
    17821 ctccctcaag gacgtagttg ttttcatatc ccagctgtct tgttatcatc caggcctgat
    17881 ttgcagcaag ggtaccgttt gagaaaaaca cgttcatttt aatatcctgg ttgaggatat
    17941 cagtgtattc gtccgacagc aggtcggagg aaggtatgtt aatggcgccg ggaagactga
    18001 acttctcaaa ttcgtcctgc ccccttacat caatgagcct cagtgaagga tccttcctga
    18061 ggatcatgtc ggcaacaact tccggagcaa cgaattgtgt ccgtgagttt gcctcggtaa
    18121 gcatctggtc agcggtcagt ttatatggat tggttttatt ctgcgggact gcagctatga
    18181 tcagtcccat tggtatcagg ataagagcga gcagattgcg tggtttcatg attttttttg
    18241 caaatttaat tttaataaca caaagatcaa tatgcttccc ttgggaattt cttttcagcc
    18301 cattcagcta cggcgaacat tgcgagtgcg accaggataa gcagcaggcc gaacacgccg
    18361 tcggagaggc cgagcacttc gctaaccttg ggttcgccca ggtattttgc aagatacagc
    18421 ttttcccacc atccgtaggt gaatgcaaaa ccgtatattc ctataaataa gccgccgata
    18481 aacaccatag catcgatctt tccgatcgcg gcaccgcaga agcttgtgcc gggacagaat
    18541 ccgcccatga tgaatcccgc acccatcaca acacccccga caatggccga agtgagatag
    18601 gtgggattga tatacacaag gttaaggtcg atccatccga aaaggctcat gaaaagcaat
    18661 ccgagcatgg cagttatcgc cgcggtgaaa aataccttaa gcacaacggc gtcgtaaccg
    18721 taaaagacgc ctgccagctt ccggctcgat gaaaaccctg agctttcaag aacccatccg
    18781 aaagcgatcc cgatgaagaa tgccaataga aaattcgtgt cttcggaaat aatttcattt
    18841 ggtgccagtg gtcccatatt ccatgtgatt taattcattt tcaacaataa tttgtcagat
    18901 tttctgtgcc ttcagggttt ctatatcccg ccctttcaat gttgttcatc ccggtgaaat
    18961 attcaggaaa ggcacccgtg tgcaggaagt tccccgtcat ttcgctgagg gtattattcg
    19021 ttgctgatat catttctata tccagttttt tctgaagaaa tacgccagca tgaatgcagt
    19081 gccgaatatt gccatcatgg taacaaaacc gccaagcgaa aggacagcca tgccgctgag
    19141 ggccgatccg ctggtgcatc ctctgcccag ctgggatccg aaaccgaaaa ggatgccgcc
    19201 ggcaagggca aagatcagtc ttctgcgcga ggttatcccg ggtgaatgct ccaccttaag
    19261 cttaagtctg ccggcaaggg cgccggatag gaatccgccc acaaccactc ccagcatctg
    19321 gaaaaccagc caggtcttca gcggatttcc cgaatgcgat tcggcgtatt cattcaggaa
    19381 cggcattttc ccggccgcgt tcctgtcggc cttttcgatg gtaaatgcaa tgacgctttt
    19441 aacggccccg cttgctccaa ggccccgccc tgaaatgaag ttcgccgata acagcaccag
    19501 gccaagcaat acaccgccca ggtagggatt caggtatctg tttgccctcg tttcttttgt
    19561 cgtagtcata taaaaaatta ttaaagtatt cctgtagttt atcagtaaag gtatctgctc
    19621 atctgtcccg cataaacaat tatgaaccgc agcatgaagc tcccgaaaat gaccaggagt
    19681 ggtgacacaa cggcgggaat gtgatatttt cttaattcca gtatttcaag cagcgccggg
    19741 ataatcaggc cgagtatcac tacaaagatc cagaatgaag cggtgtattc gcctccgaga
    19801 aacattcttg cagcctcgat ctgaacctgg gagcttgcaa gatatcccat gaacatgtgg
    19861 attataagaa aaagctctat tcctattatt gcaaggtcga tctttgcaaa ctgcttcctt
    19921 tcggccggat ctttcgacag gaagatgatc gttgcggctc ctgccgatag tcccgaagca
    19981 aggaacaggg gtccgagtat ggatgtgttc cacagcggtc ttgcattgaa ggccgacagc
    20041 aggatcccag tgtaaatccc gaggatgagc ccgaaaatca gcatgatcca tgcaagagcc
    20101 gttttatgcc tgttgaagaa gacttcgagg ttgaacagcc atttgtaacg ccagttccat
    20161 tccgggaaaa tatccctgat atgcagcgcg ctccagatga acgataccgg tgtgatcacc
    20221 ataagagtcc atgctcccca cgacatcggc gactgcatcc ttatgttggc gtaaagctgc
    20281 cagaaataca ttctgtgcct gaggtcggca aacagcgcca gcagtccgat cacgaggagg
    20341 atcggtgcaa ggaacggcgc cacacggaca
//
LOCUS       DAOOYG010000039        19547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2670_length_19547_cov_7.923661, whole genome shotgun sequence.
ACCESSION   DAOOYG010000039 DAOOYG010000000
VERSION     DAOOYG010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19547
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2670_length_19547_cov_7.923661"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PK288_05175"
     CDS             <1..947
                     /locus_tag="PK288_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HPM18346.1"
                     /translation="LKPLLKSHPDKVMLCSDDIHPEMLAERHINKIVSQLLSDGYDLF
                     DTIRSCTVNPAIHYNLDTGLLRPGDPADFLILENLASMDVKETWIDGNKVFDSGKVNF
                     TYNGAAKRNKFNCSPISKDMIKTSGIGEKIRVIQAFDGELFTRELIVNNTHAGFSGYD
                     LQNDILKLIVKDRYNDLPPAIGFIRGFGLQKGAFAGSVAHDSHNIICAGTNDEDIVTA
                     VNEIIRMKGGLAVADSGSVSSFQLQIAGIMTDRPLTEVASGYKQLSAKVKSLGCDMSS
                     PFMTLSFMALLVIPELKLSDRGLFDGRNFGFVTLSAGR"
     gene            1197..3023
                     /gene="uvrC"
                     /locus_tag="PK288_05180"
     CDS             1197..3023
                     /gene="uvrC"
                     /locus_tag="PK288_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPM18347.1"
                     /translation="MSVKTKHKEIISSLPDKPGVYQFLDATGKIIYVGKAKNLKKRVS
                     SYFAGSQSGKTTVMLGKAESLSHIVVENESDALLLENNLIKKHQPRYNILLKDDKTFP
                     WICIKNEPFPRVFSTRNVIKDGSVYFGPYTSALMVKTLLTLIRQLYKLRTCRYNLTET
                     NIKAGKFKVCLEYHLGNCKAPCVGFQTEPDYRESIVQIRDILKGHIIAVTDHLKNKMN
                     EYSNQLKFEEAQIIKEKIEILNKFRSHSAVVSNKIKNIDVFAITQDGDKFYVNYLKVV
                     EGAVIQSYTLELRMQVTEEKESLLGYAITEIRQRYSSDSPEIIVPFKPDIEMEGIRYT
                     IPKMGEKLKLLQLAERNAIYHKLEKKRRTAEIRSENRPGKNLEKVKRDLHLPSLPVHI
                     ECFDNSNIGGSSPVAACVVFRNGRPCNREYRHFNIKTVTGANDFLSMEEIVFRRYRRM
                     LDENQNLPQLVIIDGGKGQLSSAMKSIEHLGLKEKITVIGIAKKLEEIYFPGDSVPIY
                     LDKNSYTLKLIQQLRNEAHRFGINFHRHKRSSEMLKSKFDDIKGLGPKTKEKLLKNFD
                     SVEKLRMANRNELDRIIGKNKASILLKYLENEPAVTPEAQLP"
     gene            complement(3416..4027)
                     /locus_tag="PK288_05185"
     CDS             complement(3416..4027)
                     /locus_tag="PK288_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPM18348.1"
                     /translation="MIRNIIFDLGNVLISFRPAEFFRKKNYPETIVSKIVTEIFESEE
                     WLMLDNGDITTGEAIQSLSLKSSLKREEIALVFSFRTEIMFPLVENVKLLPVLKKHGF
                     KLYYLSNFPLDIFEEIKNDYYFFRYFDGGIISSEVKMSKPDVRIYHCLLDRYSLIPWE
                     SIFIDDIEKNTSAATLAGIKSFPTSGSVNIAAELERFLNIRLQ"
     gene            complement(4029..5357)
                     /locus_tag="PK288_05190"
     CDS             complement(4029..5357)
                     /locus_tag="PK288_05190"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF005301.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPM18349.1"
                     /translation="MKYEDFLVKTLGKCAVISPLKTTQREDSPVYTFVQDNERILYDV
                     RAESFENYRETGEIPVSFEKAGPKENIFFEPAKTKVGIVTCGGLCPGVNNVIRSIVNQ
                     LTYRYGISRILGIRYGYEGLVPKYNHPVVELTAEMVSEIHTSGGTFLGTSRGNQDVGQ
                     MVDTLEILNINILFCIGGDGTLRGAHAIYEEIEKRNLKIAVAGIPKTIDNDIDLIQKS
                     FGFETAFSIANDIISSAHNEAQGAYNGIALVKLMGRDSGFIAASATLAIQEVNFVLVP
                     EISFDLYGPRGFLKLLRKRLDERHHAVVVVAEGAGQDLFEPSELETDASGNIKHKDIG
                     IYLKEKIREEFKAKGIPHSIKYIDPSYIIRSAPANANDSKFCNLLAQNAVHAALAGKT
                     DFVIGYWNNEFTLLPIPRAVAKRKKIDVEGDELWWNVLEATGQPISMKNP"
     gene            complement(5627..5827)
                     /locus_tag="PK288_05195"
     CDS             complement(5627..5827)
                     /locus_tag="PK288_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HPM18350.1"
                     /translation="MEGTRRADIKPGIDVLIELTEDKRTGRLTGGKVKEILTTAPIHP
                     HGIKVKLENGLIGRIKEIIESN"
     gene            complement(6043..6945)
                     /locus_tag="PK288_05200"
     CDS             complement(6043..6945)
                     /locus_tag="PK288_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPM18351.1"
                     /translation="MSVRYLHIAVFVLFTGNIYSQVSGLSSIILAGAKPEKLSGEFAF
                     TEGPASDPRGNVYFTDQPNDRIMKWSTDDILSVFMQPSGRSNGMYFDRQGFLWSCADE
                     KNEIWKISTENKKTEIISVRFNGKPMNGPNDLWVAPDGGVYFTDPFYRRTWWDHSSMP
                     QEKECVYYLSPDRKTLISVADDLGRPNGIVGTPDGKILYVADIENNQTWSYTIKSDGT
                     LSDKKLFCGLGSDGMTIDRKGNIYLTGKGVSVFDKRGRLIGNIEIPENWTANVCFGGK
                     DHRSLFITASKSLYRLKTKMKGAY"
     gene            complement(6985..7887)
                     /locus_tag="PK288_05205"
     CDS             complement(6985..7887)
                     /locus_tag="PK288_05205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPM18352.1"
                     /translation="MRLSRRQFMTTLTAGAGGVLIGNFRQGLASPAAPLISDPFRQIV
                     LGKTGIKTTFLGLGTGFNGYNRSSAIIRAGNAKQMIRQSYEKGIRFFDCADSYGTNPY
                     TAAALKGIPRENYTLGTKMWILPGGVPDNEKGSAPVLVDRFRRELETDYIDLVQLHCL
                     TSPGWTTEHRQFMDDLENLKAKGIIRAHGVSVHSFEALEAAAESQWVDVIHVRINPFG
                     EAMDKKDPSLVAGVIEKLHRSGKGVIGMKLIGGGNYNKSTEKIDEALKYVLSLGTVDM
                     IIIGFENTGQIDDYIARIRKVQLT"
     gene            complement(8238..8681)
                     /locus_tag="PK288_05210"
     CDS             complement(8238..8681)
                     /locus_tag="PK288_05210"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPM18353.1"
                     /translation="MANVKGNIKKSQGEREYRFSLALKVRDYECDLQGVVNNANYQHY
                     LEHARHEFLLSKGISFSDLHQQGIDLIVTRAEIDYRYPLRSRDMFEVKVDVQKEGNVR
                     IVFYQGIFRIPDGKLIASAKITGAATHNGRPVFPGQDLLLKLGLA"
     gene            complement(8711..9160)
                     /locus_tag="PK288_05215"
     CDS             complement(8711..9160)
                     /locus_tag="PK288_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPM18354.1"
                     /translation="MASLEQIEEFLATESLALAGVSRNPAKFGAVAFRELRTKGMNLV
                     PVNPYAEKIMGIDVYHDVKSLPEHIRGLIIMTRKDQTAGIVREAREKGIKQIWIQQMA
                     ASREALDELEGSGINCITGECILMHYKPSGIHKFHLKLRKFFGLFPK"
     gene            9299..10582
                     /locus_tag="PK288_05220"
     CDS             9299..10582
                     /locus_tag="PK288_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM18355.1"
                     /translation="MKTNREQAGYLFSKGYSNYVFILLFLLYMFDYIDRTVITSMFTS
                     IERDWGISHTQSGMLMSAVYWAIVILTFPVSLLVDRWSRTKTIGIMAIVWSLATALCA
                     LTGNYVQLFMARLLIGVGEAGYAPGGSAVISGLYPIEKRSKMMGIWNASIPLGTAIGV
                     LLGGVIAQKLGWKHAFGLVALPGLLVAILFLFVKDYKTVDLSFLDRQNRRIKMERKDM
                     IREFISRPSIIYTYFGITAVVFVTTSLITWLSTYFQAMRGLPQDKAGTMASAVMVLAL
                     VGAPLGGIITDRWRKRRINSRLLVPSISTLISALLLFAALFFFSGLIQYILLLIMGIT
                     IMVFISGASAVTQDVIHPGLRATSYAIAVVIQNLLGSSMAPLVIGRLYDKSSIQHALS
                     VLPFVLLLGSVLFYLGSRHYEKDMGKVVKIKLEAA"
     gene            complement(10707..11681)
                     /locus_tag="PK288_05225"
     CDS             complement(10707..11681)
                     /locus_tag="PK288_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPM18356.1"
                     /translation="MPDLRNISRPVREEMARFEAYFGKTMESEIPLLKIILNYIFRRK
                     GKQMRPLLVFLSAKLNGTITESTFVAATFIELLHTASIVHDDVVDDSRERRGALSINA
                     LWNSKIAVLVGDYMLSKGLLIGIQKNSVDMLEIVSEAVQSMSKGELLQLQKARKLNIR
                     EEDYFKIISCKTAALIAACTACGARSVTDDLPTIQLMKEFGEQIGMAFQIRDDIFDYE
                     SNGLTGKPNGNDIREKKITLPLIYSLEKSSRSERKHILNVVGRKHKSRADIDEVIAFV
                     REYDGIESASRKMNYYRDQALSILDTYPESEVRRSLRDFVYYTVSRDK"
     gene            11900..12217
                     /locus_tag="PK288_05230"
     CDS             11900..12217
                     /locus_tag="PK288_05230"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPM18357.1"
                     /translation="MEIEYLKPEDLERASGMLKAIAHPVRISIISFLENNKNKTVTEI
                     HSHLGIEQSTASHHLGIMKDKGVLSSRREGKNTYYCLKNEKLTAILHCISECCQSKPT
                     VIL"
     gene            complement(12226..12834)
                     /locus_tag="PK288_05235"
     CDS             complement(12226..12834)
                     /locus_tag="PK288_05235"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HPM18358.1"
                     /translation="MSAGKKAVLFILVILVADQALKIWIKTHMTIGEEINLFGNWGRL
                     HFIENNGMAFGMEMGGKTGKLILSAFRIVAITAIGWFLNSLIRKKVNLALILTVSAIL
                     AGAIGNLIDSAFYGMIFSESHLRPAVLFPPSGGYSSFLMGKVVDMFYFPIIDTHWPDW
                     SPFRPGEPLIFFRPVFNIADSAITCGVLTILLFQKKLFRDLG"
     gene            complement(12975..13802)
                     /locus_tag="PK288_05240"
     CDS             complement(12975..13802)
                     /locus_tag="PK288_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18359.1"
                     /translation="MKRYIFVLFTLLALTVFSGCKKDKGEPPVLPPAGSMTMDFSNFE
                     VKTKDGSLPSGVKANPITNWQFAAGTALIWKAIIATTLAVPVYSFQLVHQNNTPVYLD
                     DNTWQWKYSVSLLNVTYSARLTGQILADNVRWKMFISREGAGGFEEFLWFEGISEKDG
                     SQGQWTFYESHLEKVPVLQIDWAVTGNKVGQVKYTYIKTGNPFKDSYIKFGLVTGELD
                     AFYEIHIYNTTSLQFYDVDVQWSTTNHNGRVRCQAQFGDTDWHCWDSHFSNVSCTKD"
     gene            13997..14722
                     /locus_tag="PK288_05245"
     CDS             13997..14722
                     /locus_tag="PK288_05245"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPM18360.1"
                     /translation="MEHKSTMRKIGAGIIALLIVTGSFATPPENLKNSAMEKFDIHVF
                     PALPYAYDALEPYIDARTMEIHYDKHHRAYFNNFIAAIKDTPYDSKTILQIFASVSEA
                     GDAIRNNGGGFYNHVFFWNNLSPKSGKPSAELTEAINKAFGSFDKFREAFSNSAKTRF
                     GSGWAWLYLTSDKKLAVGSTPNQDNPLMDVSTIKGIPLLALDVWEHAYYLKYQNRRPE
                     YVDAFWNVVNWDEVNRRYAEAIK"
     gene            complement(14860..16506)
                     /locus_tag="PK288_05250"
     CDS             complement(14860..16506)
                     /locus_tag="PK288_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM18361.1"
                     /translation="MEESTIRLTFPAMFSETVRRFGNCNANSFVGEKPRSYNETAGEI
                     NALISLLEKLEVRPGDKVAILSHNMPAWSAAYFSITFMGAVAVPILPDFSPHEIANIL
                     SHSEARGIYVSSALFQKLEGFSAPGFSFAINIENYSVIFSDNSSCSYDPDSRPENVYD
                     VKEEDLASIIYTSGTTGRSKGVMLTHRNIVFNALKGRVIQPITNTDRFLSVLPLSHTY
                     ENTLGLILPMLSGACVYYLKKPATPAVLLPALSEVRPTMMLTVPLIMEKIYFNKILPS
                     FREKPVVRSLYRIPFFRKKFNAIAGRKLKQTFGGELKFYGIGGAKLNNTVERFLIEAR
                     FPYAIGYGLTETSPLLAGASPARSVFDSTGPAIEGIELKINDPDIKTGQGEIWAKGPN
                     VMKGYYKEPELTSEVLTPDGWFKTGDLGVFDKNNNLYIKGRLKNMILGASGENIYPEE
                     IESLINNIRFVVESLVIQQKGKLVALVHLNLEEFEKKYQNLKQDMSRQFELKKAEILS
                     EIKDRVNSQVNKFSQLNKVVLQPVPFQKTATLKIKRYLYT"
     gene            16663..17115
                     /locus_tag="PK288_05255"
     CDS             16663..17115
                     /locus_tag="PK288_05255"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPM18362.1"
                     /translation="MSQVIRITKEFTFEMSHVLPGYDGPCRNVHGHSYRLFVTVAGIP
                     VNNALNPKNGMVMDFSDMKKIVMNEILGIFDHAVAIPGDMDREKTETLKKMFGNTIVV
                     DYQPTCENLVTDFAMRIKNQLPADVKLYSLKLYETATSFAEWFSSDNE"
     gene            17126..>19547
                     /gene="polA"
                     /locus_tag="PK288_05260"
     CDS             17126..>19547
                     /gene="polA"
                     /locus_tag="PK288_05260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPM18363.1"
                     /translation="METQPKKLFLLDAYALIFRSYYAFIKNPRVTSKGLNTSAIFGFL
                     LTLREVLQKQKPSHIAVVFDTPEPTFRHEMFSGYKATRDETPEDIKIAVPYIKRLLEA
                     YRIPVIACPGYEADDVIGTLANKASEAGFITYMMTPDKDFAQLVTDKIFMYKPSRGGN
                     ESVLWGVAEVKNEFSVNTPVQVIDILALMGDTADNIPGAPGVGPKTAMKLISEYGSVE
                     EVIRNTDKLKGKLKEAIGNNIPQIELSRKLAAIDVNAPVDFMEEGLRIKNPDRNMLKS
                     LFEELEFRTIGAEILSGAEYSDTLSFPEGNTAGEFTATAQGTLFDQNASPVATPVATI
                     ENTPHEYLLIEGNNELREFVEKVLLLKEICFDTETTHINPFDSELVALAFSWTRGKGY
                     LVHFPESRQETAAMMEILRPIFESDRILKIGQNIKFDIQVLANYGIDVKGPLFDTMIA
                     HYLLEPDMRHNMNLLAETYLSYSPVHIESLIGEKGAGQKSMRSVSVDKIKEYAVEDAD
                     VTCQLKEIFMPVLKSEGLIDLAETVEMPLIRVLADMERNGVRLNQDDLRSISEGLREE
                     IIILEKEIHSLAGTEFNISSPKQLGDILFLKLKLDDKARLTKTRQFVTNEEILQRLTG
                     RHPIVSKVLEYRGLKKLLSTYLEPLPQMIDPKTGRIHTSFRQAVTSTGRLSSDNPNLQ
                     NIPVRDERGREIRKAFVPEDGNLFFSADYSQIELRLMAHLSKDKGMIEDFLSGSDIHA
                     ATASKIFGVEIGDVTREMRSRAKTANFGIIYGISSFGLSERLTIGRKEAKELIDGYFQ
                     SYPGVKRYM"
BASE COUNT         5481 a         4348 c         4255 g         5463 t
ORIGIN      
        1 ctcttaaacc tcttctgaaa agccaccctg acaaagtaat gctctgcagc gacgatattc
       61 atcccgaaat gctggcagaa cggcacataa ataaaattgt ttcgcaactg ctcagtgatg
      121 gctatgatct gttcgacaca ataagaagct gcacggtcaa ccctgcaatt cactataatc
      181 tggatacagg cctgctcaga ccaggcgatc cggcagattt cctgatattg gaaaaccttg
      241 cttcaatgga tgtgaaggag acctggatcg acggtaataa agtgtttgat tcgggtaaag
      301 tcaactttac ctataacggg gcagcgaaaa gaaataaatt caactgctcc ccaataagca
      361 aggatatgat taaaaccagc ggaatcgggg aaaaaatcag ggtgattcag gcttttgacg
      421 gggaactttt tacaagggaa cttattgtaa ataacaccca tgctggtttt tccggatatg
      481 atcttcagaa tgacatattg aaattaattg tgaaggaccg ctataacgac ctgccgccag
      541 ccatcggatt tatcagggga ttcggattgc agaagggcgc ctttgccggc tcggttgctc
      601 atgacagcca taatattatt tgcgcgggta caaatgatga ggacatcgtg accgcagtaa
      661 atgaaataat caggatgaaa gggggtctgg ccgttgcaga ttcaggcagt gtcagttcgt
      721 ttcagcttca gatcgcaggc atcatgacag accggccgct gactgaagtt gcctcaggct
      781 ataaacagct ttccgcaaaa gtgaaatccc ttggttgtga tatgtcgtct ccctttatga
      841 ccctctcatt catggcactg cttgttatcc cggaattgaa gctgagcgac aggggactgt
      901 ttgacggacg taatttcgga ttcgtcactt tgtcggccgg cagatagctc ccgtacaacc
      961 atttgcagtg aattccatca gcccgtttga tcaattaaac agatccactg gtttattcag
     1021 gaatagctgc cccggataac ataccggatc cgctgatcat tacagccgtg ggtaattctt
     1081 tcatttaaac tggcccggaa cctaaaataa tgcccgaaaa acattacaac cccgataact
     1141 gttacttttg cagaaatgca aacgcatctg ttactatctt tgcatagagg tggataatgt
     1201 cggtgaaaac aaaacataag gaaataatat cttcccttcc tgataaacct ggagtgtatc
     1261 agtttctgga cgcaacaggg aaaatcattt atgttggcaa ggcaaaaaac ctgaaaaaaa
     1321 gagtctcatc ttattttgca ggcagccagt cgggcaaaac aacagtcatg ctgggaaaag
     1381 ctgaatccct gagccatatt gttgttgaaa atgaatcgga tgccctgctt cttgaaaata
     1441 atcttattaa aaagcatcag ccccgctata atatactatt aaaggatgac aaaacctttc
     1501 catggatttg cattaaaaac gaaccatttc cgcgcgtatt cagtacgcgg aacgtaataa
     1561 aggacggatc tgtctatttc ggaccatata cctcggccct gatggtaaaa accttactga
     1621 ccctcatccg gcagctgtat aagctgagaa catgccggta caacctgacg gaaacaaaca
     1681 taaaagccgg aaaattcaag gtctgtctcg aataccatct tgggaactgt aaagctccgt
     1741 gtgttggctt ccagacagag cccgactaca gggaaagcat tgttcagatt agggatatcc
     1801 tcaaaggcca tataattgcc gttaccgatc atctgaaaaa taagatgaat gaatactcga
     1861 accagctgaa atttgaagag gcccagatca ttaaggaaaa aatcgagatc ctcaataagt
     1921 tcaggagcca ttcagcagtg gtgagcaaca agattaaaaa catcgacgta ttcgctatta
     1981 cacaggacgg tgacaaattc tatgtcaact atctcaaggt tgttgaaggt gctgtgatcc
     2041 agagctatac tcttgaatta agaatgcagg tgactgaaga aaaggaaagc ctcctgggtt
     2101 atgcgataac cgaaatacga cagcggtatt caagcgattc tcctgaaatt atagtcccgt
     2161 tcaaacccga tattgaaatg gagggaatca ggtatacaat accaaaaatg ggtgaaaagc
     2221 tcaaactgct gcagctggcc gagcggaatg ccatttacca taaactggaa aaaaagagga
     2281 gaactgccga aatcaggtcc gaaaaccgcc cgggcaaaaa cctggaaaag gtgaaaagag
     2341 atcttcattt accatcatta ccggtccata tcgaatgttt tgacaattcg aatatcggtg
     2401 gctccagtcc ggttgcagca tgtgtggttt ttcggaacgg caggccgtgc aacagggaat
     2461 accgtcactt taatattaaa accgttaccg gtgccaatga ttttttatca atggaggaaa
     2521 ttgttttcag aaggtacagg agaatgcttg atgagaacca gaatcttccg cagctggtaa
     2581 ttatcgacgg cggtaaagga cagctgtcgt ctgccatgaa aagcatagaa catctcggtc
     2641 taaaggaaaa aatcacagta ataggaattg caaaaaaact cgaagaaata tattttcccg
     2701 gcgattccgt acccatttac cttgataaga attcatacac cctgaaactt attcagcagc
     2761 tcaggaacga agcacaccgg tttggaataa actttcatcg tcacaaacgc tcttcagaaa
     2821 tgctgaaatc aaagtttgac gatattaaag gcctcggacc gaaaacaaag gaaaaactgc
     2881 tgaagaactt tgattctgtc gaaaaactta gaatggcaaa cagaaatgag cttgacagaa
     2941 tcatcggcaa aaataaggct tcaattcttc tgaaatactt ggaaaacgaa cctgcggtaa
     3001 cacctgaagc acaacttcca tagaagcaga taaataattg attatttgat ttttagatat
     3061 tcccgaatga atggttaact ccatctggct gcaatattcc ggacttatct gtgcagatgc
     3121 ctgaaatttt caagggaaaa caaggaaacc ctacctgccc atgacagaat aaccccggat
     3181 aacttccggt ttcaatcagt aaggttttaa agagtgtata taccctgcta tctgttaagg
     3241 tacttatttg aatccaattt actgcagaaa cgaaaatgaa atcaaaccgg gatgatctgt
     3301 gcagttattc cttcagttgg tctaatgacc gatcagcagg tacgcacctt cccccggcct
     3361 gtttgaatat ccgcacgcga catttataat agacgggcgt actggtttta tggcattatt
     3421 gcagcctgat atttaaaaaa cgttcaagct cagctgcgat attaaccgat ccggatgtgg
     3481 gaaatgattt tattccggcc agtgttgctg cgcttgtatt tttttcaata tcatcaatga
     3541 atattgattc ccagggaatc agtgaatatc tgtcaagaag acaatggtaa atacgcacgt
     3601 caggttttga catttttact tccgaggaaa ttattcctcc gtcaaaatac cggaagaagt
     3661 aataatcatt ttttatctct tcaaaaatgt caagggggaa gttcgacaga taatatagct
     3721 taaaaccatg ttttttaagc acaggaagga gttttacgtt ctccacgagg ggaaacatta
     3781 tctcagtgcg aaaactgaac acaagggcta tttcctccct tttcagggat gatttaaggg
     3841 agagtgactg aatcgcttcg ccggtggtta tatctccgtt gtcgagcatc agccattctt
     3901 cactttcgaa tatttcagtg acaattttgc tgacaatagt ttcaggatag ttcttctttc
     3961 tgaaaaattc tgcgggacgg aagctgatca gcacatttcc caggtcaaaa ataatattcc
     4021 tgatcatgtc agggattttt catactgatg ggctgacccg ttgcctccag cacgttccac
     4081 cacagctcat caccctcaac atcgatcttt tttcttttgg ccacggccct gggtatagga
     4141 agcagtgtaa attcattgtt ccagtaccct attacaaagt cagtttttcc ggccagtgct
     4201 gcatgtacgg cattctgggc aaggaggttg cagaatttgc tgtcattggc attggccggc
     4261 gcgctcctga taatgtagct gggatcgata tacttgattg aatgaggtat gcctttggcc
     4321 ttgaactctt ccctgatctt ttccttcagg tagatgccaa tgtccttgtg cttgatattt
     4381 ccggaagcat cggtttcaag ttcggatggt tcgaacagat cctgccctgc tccttcggct
     4441 accacaacca cggcatggtg cctttcgtcg agccttttcc tgagaagttt gagaaatccc
     4501 ctgggaccgt acaggtcaaa ggatatttcc ggaaccagta caaaattcac ttcctgaatg
     4561 gcaagtgtgg cacttgcagc tataaaaccg gaatccctgc ccatcaattt gaccagtgcg
     4621 atcccgttgt atgccccctg ggcctcattg tgggcactgc tgattatgtc gttggcgatt
     4681 gaaaaggctg tttcaaatcc gaatgatttc tgtataagat caatatcgtt gtcaatggtt
     4741 ttcgggatcc cggcaactgc tatcttgaga ttcctttttt caatttcctc gtagatggcg
     4801 tgggcacctc tcaatgtacc gtcacctcct atgcagaaaa ggatatttat attgaggatc
     4861 tccagtgtat ccaccatctg gccgacatcc tggttgccgc gtgatgttcc gagaaaagta
     4921 ccgcccgatg tatgaatctc actcaccatt tcagcggtca gttccacaac cggatggttg
     4981 tatttcggaa ccaggccttc atacccgtac ctgataccga gtatcctgct gatgccgtac
     5041 ctgtatgtga gctgatttac aatgctccgg atcacattat tcaccccggg gcaaagcccg
     5101 ccgcaggtaa caattcccac tttcgtcttc gcaggctcga aaaagatatt ttccttgggc
     5161 cctgctttct cgaatgacac cggaatttca cccgtttcac ggtaattctc aaaactttct
     5221 gcccttacat catacaggat cctttcgttg tcctgaacaa aggtatacac cggactgtct
     5281 tcacgctggg ttgttttcag gggggaaatc actgcgcatt tcccaagggt tttcaccaga
     5341 aaatcttcgt atttcataat attgttcttg aaaatttgcc tatgccgtca atgccgctga
     5401 ataactaaaa ttccttaatg gcgcggcatg tttttccgtt aaaggtaaaa taataaaacc
     5461 cagaaaaaaa catttaagta ttccagaaga aaaaaaactg caaaaatcac ggttgcactg
     5521 tccggatgac ttatacagat ccgacctgta tttccggcac ataaggtgga aaaacaatga
     5581 ttccgtgagt tttgcaggac aagcgcgaaa taaggatgat aatgaatcag ttcgattcga
     5641 ttatctcttt tattctgccg ataagtccgt tttccagctt taccttgatc ccgtggggat
     5701 ggattggagc ggtagtaagt atttccttga ctttcccgcc tgtaagtcga cctgtccgct
     5761 tgtcctccgt taattcaatc agcacatcaa ttcccggctt tatgtcagcc ctgcgtgttc
     5821 cttccattat acctggtttt gaattaaact aatatgaacc ccaaatgttt ttcaaacccg
     5881 atagggatga aagtatttcc aaaaacatat gagacccgtt taatcttgaa aaaatctatt
     5941 aaatattgaa tataaattct acgatcttca aatattgtta caattttaaa tattttattt
     6001 tataacaagc aaaaatttta atactttttg tgttaaagta ttttaatatg ctcctttcat
     6061 tttcgtcttt agcctgtaaa ggcttttgct tgccgtaata aataatgatc tgtgatcctt
     6121 tcctccaaaa cagacattgg ctgtccagtt ttcaggaatt tcgatatttc ctatcagtct
     6181 accccttttg tcaaaaacgc ttactccctt tccggtcaga tatatgtttc ccttcctgtc
     6241 tattgtcatt ccgtcagagc cgagcccgca gaaaagcttt ttatctgaaa gggttccgtc
     6301 ggattttatc gtgtatgacc aggtctggtt attttcaatg tcagccacat aaaggatctt
     6361 accgtcaggg gtgccgacaa taccgttagg cctgccaagg tcatctgcta cgcttatgag
     6421 agtcttgcgg tcgggactga gataatagac acattctttt tcctgaggca ttgaggaatg
     6481 atcccaccag gtccgcctgt aaaacggatc ggtgaaatag actcctccgt cgggagcaac
     6541 ccagagatca tttggtccgt tcatcggctt cccgttgaat ctgacggaaa tgatctctgt
     6601 ttttttattt tctgtgctga tcttccatat ctcgttcttt tcatcggcac atgaccaaag
     6661 gaatccctgc ctgtcgaaat acatcccgtt cgatctgccg gaaggctgca tgaaaacaga
     6721 caatatgtca tcggtactcc atttcatgat cctgtcgttt ggctgatcag tgaaatatac
     6781 gttcccgcgg ggatcggagg ccgggccctc ggtaaaggca aattcaccgg aaagcttttc
     6841 aggctttgct cctgccaaaa taatggatga cagtccgctg acctgggaat agatattgcc
     6901 ggtgaaaagg acaaaaactg ctatatgcag atatcttaca gacatacgga taatgttatt
     6961 ctgacatggt ttatcctgta cagctcatgt tagctgaacc ttccttatcc ttgcaatgta
     7021 atcatctatc tggcctgtgt tttcaaaccc gatgataatc atatcgacag ttccgagact
     7081 gagaacatat ttgagggctt cgtcaatctt ttcggtgctt ttattgtaat ttcctcctcc
     7141 gatcagtttc atccctatca ctcccttccc cgatctgtga agcttttcaa ttaccccggc
     7201 aacaagggaa ggatcctttt tgtccatagc ttccccgaaa ggattgatcc gcacatgtat
     7261 tacatcaacc cactggcttt ctgcggccgc ttcaagtgct tcaaaggagt ggactgatac
     7321 cccgtgggcc cggattattc cttttgcctt gaggttctct aggtcatcca tgaattgcct
     7381 gtgttcggtt gtccatcctg ggcttgtcag gcaatgcagc tggacgaggt cgatatagtc
     7441 ggtttcaagt tccctgcgga agcgatcaac caacaccggt gcactaccct tttcattgtc
     7501 aggcacccct ccgggaagga tccacatttt tgtgccaagg gtgtaatttt cccttggaat
     7561 tcctttaagt gcggccgcgg tatatggatt ggttccatac gaatctgcac aatcaaagaa
     7621 ccgaatgcct ttttcatacg actgccgtat catctgtttg gcattcccgg ccctgattat
     7681 tgccgaacta cggttatagc cgttaaaacc tgttcccaat ccaaggaaag tggtttttat
     7741 gcccgttttg ccaagtacga tctgtctgaa cggatcggat atcaatggag ccgctggtga
     7801 agcaaggccc tgtctgaagt ttcctatcag cacaccacca gcaccggctg tcagtgtggt
     7861 catgaattgt cttctggaga gtctcatctt ttcaattttc tgaaatgtga tttatatggt
     7921 cctgtaaaat aacaaaaaaa atgtaacctg ggttcatggt tccttttatt taaagcccga
     7981 ttttaatttc tggaataaca ggcgacctgc gctcagggta cttacatcgt tcctgggcag
     8041 accacgaatt tatttccctt cccttatgag gctgcatctt aaaggccaga ttggtatcat
     8101 tgtttaaaca cgggattcat cagcaacaga tgatattcat aactgcttaa gatgttcagc
     8161 ggaaaagcat tgcagacctg atatttgtgg aggatgccat acagatggat aataatctta
     8221 atgaaacagg taagttttta agcaagtccc agtttcagca ggagatcctg gccgggaaaa
     8281 accggccttc cattatgtgt ggcggcaccg gttatttttg cactggctat cagcttgccg
     8341 tccggtatcc tgaaaattcc ctggtaaaaa acaatccgga cgtttccttc cttctgcaca
     8401 tccaccttca cttcaaacat gtcgcggctg cgaaggggat aacgataatc aatctctgcc
     8461 cttgtgacga tcaaatcaat gccctgctga tggagatcgg agaaactgat tcctttcgaa
     8521 agcaggaatt catggcgcgc atgttcaaga tagtgctgat aatttgcgtt attgaccaca
     8581 ccctgcaggt cacattcata gtcccttacc ttaagagcca gactgaagcg gtattccctt
     8641 tctccctggg attttttgat attgcctttg acattggcca tactcttcct gaattgaatt
     8701 tttatctcat ttattttggg aacagtccga aaaactttct cagcttcagg tggaatttat
     8761 gaatacctga tggtttgtag tgcatgagga tgcattcccc ggtaatgcag ttaattccag
     8821 aaccttcaag ttcatccaac gcttccctgc tggcggccat ctgctggatc cagatctgtt
     8881 ttataccttt ttcccttgct tcccttacta tgcccgcagt ctggtccttc ctggtcatta
     8941 tgatcaggcc cctgatatgc tcaggaaggc ttttcacatc gtgatacaca tcaatgccca
     9001 tgatcttttc agcgtacgga ttgacgggaa caagattcat accttttgtc cttagctccc
     9061 tgaatgccac ggctccgaac tttgcgggat tccttgagac ccctgcaagt gccagcgatt
     9121 cagtagccag aaattcctct atctgttcca ggcttgccat tgttttccga gaattaaaat
     9181 caagaaaata aaaccttcag attcagtttc agctaagtat aaagacttaa aattaaaata
     9241 aattcaacaa caattataat tttatattta cttggcggga ataaaacctg aaatattaat
     9301 gaaaaccaac cgggagcaag ctggatacct tttttcaaaa ggctattcca attatgtatt
     9361 catactgctg tttctcctgt atatgttcga ttatatcgac cgtaccgtaa tcacctcaat
     9421 gtttacctcc attgagaggg actggggtat ttcccataca caaagcggga tgctcatgtc
     9481 ggcagtatac tgggccattg tgatcctgac ctttcctgta tcccttcttg ttgacaggtg
     9541 gagcaggact aagaccatcg ggataatggc cattgtatgg agtcttgcca ctgcactgtg
     9601 cgcccttaca ggcaattacg tccagttgtt catggcccgg cttctgattg gtgtgggcga
     9661 ggccggttat gctccgggcg gaagtgcagt gatctcaggt ctctatccca ttgagaaacg
     9721 ctcaaaaatg atggggatct ggaacgcatc aataccgctt ggaacagcga ttggagttct
     9781 tctgggaggt gtcatcgccc agaaactggg atggaaacat gccttcggac tggttgccct
     9841 gccgggtttg ctggtagcaa tactgttcct tttcgttaag gattacaaaa cagttgatct
     9901 ttcattcctc gaccggcaga acaggaggat taagatggaa agaaaggata tgataaggga
     9961 gttcatttcc agaccttcaa taatctacac ctactttggg atcactgcag tcgtttttgt
    10021 aacaacatca cttattacat ggctctcaac ttatttccag gccatgagag gattgccgca
    10081 ggacaaggcc gggacaatgg caagtgccgt tatggtgctt gccctggtgg gagcccccct
    10141 aggtggtatt attaccgaca ggtggagaaa aagaaggatc aattccaggc tgctggtacc
    10201 ctcaatttca acattgatct cggccctgct gttatttgcc gccctgttct ttttcagcgg
    10261 acttattcag tacatactgc ttctcattat gggaatcacc ataatggtat ttatttcagg
    10321 tgcttctgca gtgacccagg atgtgattca tccgggactg cgggcaactt catatgcaat
    10381 tgccgtggtt atccagaatc tccttggatc atcaatggca ccactggtca taggcaggtt
    10441 gtatgacaaa tcttcaattc agcatgccct gtcggttctg ccctttgtcc ttttattggg
    10501 ctcggtactg ttttacctgg gctcaagaca ctatgaaaaa gacatgggaa aggttgtaaa
    10561 gatcaaactg gaggcagcct gacagtcagg atgtcattca tgggtccgga tatcctgaaa
    10621 ggaacattgc tctaacccgg cttcaccggc aaaacggggt aacctaaaca atgagctgaa
    10681 caaatgacct aaagtccttc attatatcat ttatcccttg aaacggtata ataaacaaaa
    10741 tccctgagcg atctcctgac ttcggattca ggataagtgt cgaggattga aagcgcctga
    10801 tcgcggtaat aattcatttt ccggcttgcg gattcaatcc cgtcatattc cctgacaaat
    10861 gcaattactt catcaatgtc agccctgctc ttatgcttgc gtccgacaac attaagaata
    10921 tgcttccttt ctgagcggga agacttttca agagaataga taagaggaag ggttatcttt
    10981 ttttccctga tatcattccc attgggtttt ccggtaagtc cgttgctttc atagtcaaaa
    11041 atatcatccc tgatctggaa tgccattcca atctgttctc caaattcctt cataagctgt
    11101 attgtcggca ggtcatcggt aaccgatctg gctccgcagg cggtgcatgc ggcaataaga
    11161 gccgcggttt tgcagcttat gattttgaag tagtcctcct ccctgatatt gagtttcctt
    11221 gccttttgaa gctgaagcag ttctcccttg ctcattgact ggacagcttc tgatactatc
    11281 tcgagcatgt ccacgctgtt tttctgaatg ccgatcagca ggcccttgga caacatataa
    11341 tctcctacca ggacggcaat ttttgaattc cacagggcat tgattgacaa ggcgccgcgt
    11401 cgctctctgg aatcatcaac gacatcatca tggactattg aggcagtatg aagaagctca
    11461 ataaatgttg ctgccacaaa tgtggattcc gtaattgttc cgttgagttt tgcggagagg
    11521 aaaacaagca ggggcctcat ctgtttgcct ttacgccgga atatataatt aaggattatc
    11581 ttcagtagag ggatctcgct ttccatggtc tttccgaagt aagcttcgaa ccgggccatc
    11641 tcctccctga caggtctgct gatattcctc agatcaggca tgaaaattag tttggtcaaa
    11701 tataggataa ataatcaatg agaaaccggc aattgatttt tatctgtttt aaggcaactg
    11761 ctgaaactta atgctttttc gtgccgtgat gaaatcattt tatttccgga aaaccgcttg
    11821 taatttttcc atccgggcat gacaaatttt tctatgacaa aatatcatat atttgcttaa
    11881 cgaatctgta ttaaaataaa tggagattga atatttaaaa cctgaagatc tggaaagggc
    11941 atcaggtatg ctgaaggcaa ttgcccaccc ggttagaatt tcaataatca gctttcttga
    12001 aaacaataag aataaaactg tgacggaaat tcacagtcat ctggggattg aacagtcaac
    12061 tgcatcacat catctgggaa ttatgaagga taaaggtgtt ttgtcatcgc gcagggaggg
    12121 taaaaacact tattactgcc ttaaaaatga aaaactgaca gccattcttc actgcatctc
    12181 cgaatgttgt cagtccaaac caaccgtaat cctttagggt tgaacctatc caagatccct
    12241 gaaaagcttt ttctgaaaca gtaaaatcgt cagaactccg caggttattg ccgaatcggc
    12301 tatattaaag acgggtctga agaaaatcag aggttcacct ggtctgaaag gagaccagtc
    12361 aggccagtga gtatcaatta tcgggaaata aaacatgtct acaacttttc ccataagaaa
    12421 tgaggaataa cctccggaag gaggaaacaa tactgcggga cgcagatgac tctcgctgaa
    12481 tatcattccg taaaatgcac tgtcgatgag attccctatt gctcctgcca gtattgcact
    12541 caccgtcagg atcagagcca ggttaacttt tttccttatc aggctgttga gaaaccatcc
    12601 gatcgcagta atagcaacta ttctgaatgc gcttaaaata agcttccccg ttttaccgcc
    12661 catttccatt ccaaaggcca tcccgttgtt ttcaatgaaa tgaagccggc cccagttacc
    12721 gaagagattg atttcctccc cgattgtcat atgtgtttta atccatattt taagcgcctg
    12781 atcggcaaca agaataacaa gaataaataa aactgccttt tttcctgctg acatttccga
    12841 attattgtgg gccgcaaaat agtaaaaaaa tcagagcgat aaaaaacccc ggttattttg
    12901 agaagagaaa aaaactgtat tggattcctg attcgttcgg gaatccaata cagtcataat
    12961 tgaaatatta ttctttaatc ttttgtgcag gagacgtttg aaaaatgact atcccagcag
    13021 tgccagtccg tatccccgaa ctgagcctgg catctgaccc tgccgttatg atttgttgtg
    13081 ctccattgta catccacatc atagaactgg agagaagttg tattgtaaat atgtatttca
    13141 taaaatgcgt ccagttcgcc ggtgaccagg ccaaatttta tataactatc cttgaatgga
    13201 ttgccggtct ttatatacgt atattttacc tggcccacct tgttgccggt tactgcccag
    13261 tcaatctgta aaaccggaac cttttccaga tggctttcat agaaagtcca ctgaccctgg
    13321 gagccatctt tttcagagat accctcgaac cacaggaact cttcaaaacc gccggcaccc
    13381 tcccttgaga taaacatctt ccagcgtaca ttatctgcaa ggatctgacc ggtcagacgt
    13441 gcagagtagg tcacattcag aagactcaca ctgtatttcc attgccaggt attgtcgtca
    13501 agatatacgg gggtgttgtt ctgatgcacc agctggaatg aatagaccgg tactgcaagc
    13561 gttgtggcga taattgcttt ccagattaat gcagttccgg cagcaaactg ccagttggtg
    13621 atgggattcg ctttgacccc ggaaggtagg gaaccatctt tggttttaac ttcaaaattg
    13681 ctgaaatcca ttgtcattga accggcagga ggtaaaaccg gaggttctcc cttgtctttc
    13741 ttgcagcctg aaaatactgt cagtgcaagc agggtaaaaa gaacaaaaat atatctcttc
    13801 attgtctgaa aaattaattg atgattatat gcaaacggta aaactgtcat aaaattatcg
    13861 aacgtcaatc cggatatttt tgagaataaa gccaaacctg tggctaccag gtattctcag
    13921 caagaattgt accggaaatt ctccggataa ctgaacatta tcataatttt gttgtttaaa
    13981 caaaaaataa acaaaaatgg aacacaaaag tacaatgaga aaaatcggag cggggatcat
    14041 tgcattactg attgttacag gatcgtttgc aacccctccg gaaaacttaa aaaattctgc
    14101 catggagaaa tttgatattc atgtattccc ggctttgcca tatgcctatg acgcattgga
    14161 accatacatt gatgcacgga caatggagat acattatgac aagcaccatc gtgcatattt
    14221 caacaatttt attgcagcca tcaaggacac gccgtacgac agtaaaacca tcctgcagat
    14281 atttgccagt gtttctgaag cgggagatgc cataagaaac aatggcggag gtttctacaa
    14341 tcatgtcttt ttctggaaca acctctcgcc aaaatcaggc aagccgtctg ccgaactgac
    14401 ggaagccatc aataaggcat tcggttcatt cgacaagttc agggaagcct tcagcaattc
    14461 cgcaaaaaca cgatttggaa gcggctgggc ctggctgtat cttacatccg acaagaagct
    14521 ggccgtggga agcacaccca atcaggacaa ccctctgatg gatgtatcca cgataaaggg
    14581 gatccccctg ctggctcttg atgtttggga acatgcctat tatcttaaat atcagaaccg
    14641 ccggcccgag tacgttgacg ctttctggaa tgttgtcaat tgggatgagg taaacaggag
    14701 atacgccgaa gccattaaat aatacaggaa gatatttaat cgatttaagg ggagctgtcc
    14761 tgatctgatt taatacctga agcagatcat catggctccc ttcttatttt aatcgatgat
    14821 gaagcgcaaa gggctgccgg gtaacctggt tccggaggat caggtataca ggtatctttt
    14881 aattttcagt gtggcagttt tctgaaaagg taccggctgc agcacaacct tgtttagctg
    14941 actgaactta ttaacctggg aattaacccg atccttgatc tcggagagaa tttccgcctt
    15001 ctttaattca aactgcctcg acatatcctg tttaagattc tggtatttct tctcaaactc
    15061 ctccagattg agatgcacca gtgcaacaag ctttcccttt tgctggatca cgagcgattc
    15121 gaccacaaat ctgatattgt ttatcagcga ttcaatctcc tcggggtaaa tgttttctcc
    15181 gctggctccc aggatcatat ttttcaacct gccttttatg tagaggttgt tatttttatc
    15241 gaatacccct agatccccgg ttttgaacca gccatcgggt gtcaggactt cactggtgag
    15301 ttcaggttcc ttgtaatatc ctttcattac atttgggcct tttgcccaga tttccccctg
    15361 tccggtcttt atgtcaggat cgtttatttt cagctctatt ccctcaattg ccggacccgt
    15421 ggagtcgaat accgacctgg cgggactggc gccggcaagc aggggagacg tttccgtaag
    15481 accatatcct attgcatagg gaaaacgggc ctcaatgagg aacctttcca ccgtattatt
    15541 gagttttgcg ccaccgattc cataaaactt aagctctccg ccgaaagtct gtttcagctt
    15601 tctgccagca attgcattaa acttctttct gaaaaacggg atcctgtaca ggcttcttac
    15661 tacaggtttt tccctgaagg atggtagtat tttgttgaag tagatctttt ccattatcag
    15721 cggtacggtc agcatcatcg taggcctgac ttcactgagg gccggaagga gaacagcagg
    15781 tgttgccggc tttttcaggt aatagacaca ggcaccggaa agcatgggca gtattaaacc
    15841 gagcgtgttc tcatatgtat gggatagcgg caggacagac aggaatctgt cggtattggt
    15901 tatcggctgt ataaccctgc ccttcaaggc gttgaaaaca atgttccggt gtgtcagcat
    15961 tacccccttc gaccgcccgg tggttcctga agtgtatatt attgatgcaa gatcttcttc
    16021 tttgacatca taaacgtttt caggccggga atccgggtcg taactgcatg atgaattatc
    16081 cgaaaaaata actgaataat tctcgatatt gatggcaaat gaaaaaccgg gggcactgaa
    16141 gccttccagt ttctgaaaaa gggcagatga aacgtaaatg cccctggctt ccgaatgact
    16201 cagtatattt gcaatctcat gcggtgaaaa atcaggcagg attggtactg caacggctcc
    16261 catgaaggtt atggaaaagt atgcggcact ccatgcaggc atattgtgac tgagtatggc
    16321 aaccttgtcg ccgggcctta cttccagctt ttcaagcagc gaaatcaatg catttatctc
    16381 accggcagtt tcattgtaac tcctgggttt ttcaccgaca aaggaatttg cattgcagtt
    16441 gccgaatctt ctgactgttt cactgaacat tgcagggaat gtgagcctga tggtactttc
    16501 ttccatataa gtttttttgt caacggaaac gcaaagatgc ataaatatat ttcaactttt
    16561 cattaacatg cgaaatatta attcatcggg aaagaacggc cacccgattt aaaacttttt
    16621 tgacataagt tggttaaatt tgtacaactg tttacaaaag agatgagcca ggtaataaga
    16681 ataaccaagg aattcacctt tgaaatgtcg catgttctgc cgggatatga cggaccctgc
    16741 aggaatgttc acggacattc atacaggctg ttcgtaacgg tggcaggaat accggtgaat
    16801 aatgccctga acccaaaaaa cggtatggta atggacttct ccgacatgaa aaagatcgtc
    16861 atgaatgaaa ttttaggcat ctttgatcat gcagtggcaa tccccggtga tatggacagg
    16921 gaaaaaacgg agacactgaa aaaaatgttt ggaaatacca tcgtggttga ctatcagcca
    16981 acatgtgaga atcttgtaac ggattttgca atgaggataa aaaaccagct cccggccgat
    17041 gttaagctat acagtcttaa gttgtatgaa actgctacct catttgcaga atggttttca
    17101 tccgacaacg aataaccgga atttgatgga aacacaacct aagaaacttt tcctgcttga
    17161 cgcttatgcg ctgatcttcc gctcatacta cgcattcata aaaaatccca gggttacttc
    17221 aaagggattg aacacttcag ccatattcgg atttcttctt acgctgagag aggttcttca
    17281 gaaacaaaaa ccttcacata ttgcagttgt ttttgacact cccgaaccca ctttcaggca
    17341 tgagatgttc agcgggtata aggctacgcg tgatgaaacg ccggaagata taaaaattgc
    17401 cgtaccatac ataaagaggc tgctggaggc ttacaggatt cccgttatcg cctgccccgg
    17461 gtatgaagcg gatgatgtga tcggtacgct ggcaaataaa gcttctgaag cagggttcat
    17521 aacttatatg atgacccccg acaaggattt tgcccagctg gtaacagata aaatattcat
    17581 gtataagccc tcaaggggcg gcaacgaatc agtattatgg ggtgtagcag aagtaaaaaa
    17641 tgaattttca gtaaacacac cggttcaggt aatcgatata cttgcattga tgggcgatac
    17701 ggcagacaat attcccggtg cccccggcgt gggaccaaaa accgcaatga agcttatttc
    17761 agaatacgga tcggttgagg aagtcatcag gaatactgat aagctgaaag gaaaactgaa
    17821 agaggcaatc gggaacaata ttccgcagat cgaactttcc aggaaactgg ctgcgataga
    17881 tgtaaatgca cctgttgatt tcatggaaga gggtctgagg atcaaaaatc ccgacaggaa
    17941 tatgctgaag tcgctttttg aagaacttga attccggact atcggcgccg agatcctttc
    18001 cggggcagaa tacagcgata ctctttcatt cccggaaggc aatacggcag gagagttcac
    18061 tgccactgcg cagggaacac tgtttgacca gaatgcatca cccgttgcga cgcccgttgc
    18121 caccattgaa aataccccgc atgaatattt actgatagag ggtaataatg agttaaggga
    18181 atttgttgaa aaagtccttc tgctgaaaga aatctgtttt gatacggaaa cgacccatat
    18241 caatccgttt gattcggagc ttgtcgccct ggcattttca tggaccaggg gaaagggata
    18301 cctggttcat tttccggagt cacggcaaga aacagctgca atgatggaaa tcctccggcc
    18361 catttttgaa agcgaccgga tacttaagat aggacagaat atcaaatttg atattcaggt
    18421 acttgcaaat tatggcattg acgtgaaggg accgcttttt gatacaatga tcgcccatta
    18481 ccttctcgaa cctgacatga gacataatat gaatcttctg gcggaaacct acctttcata
    18541 cagtcctgtt catattgaat ctctgatagg cgaaaaaggc gccggtcaga aaagtatgag
    18601 atcggtgtca gttgataaaa ttaaagagta tgccgttgag gatgctgatg ttacctgtca
    18661 gcttaaggaa atcttcatgc cagtcttaaa atcggaagga ctgattgatc tggcggaaac
    18721 cgtggaaatg cccctcatca gggttcttgc cgacatggag cgcaacggtg taaggctgaa
    18781 tcaggatgat ctcagatcaa tttccgaagg cctgagggaa gagatcatta tccttgagaa
    18841 ggagatacac tctcttgccg gcactgaatt caacatctct tctccaaaac agcttggcga
    18901 catccttttc ctgaagctaa aactcgatga caaggccagg cttacaaaaa ccaggcagtt
    18961 cgtaacaaat gaggaaatac ttcaacggct tacaggccgt catcccattg tcagcaaggt
    19021 tctggaatac agggggctta aaaagctgct ttcaacttat cttgaaccat tgccccagat
    19081 gattgatccg aaaacgggaa ggatccatac ttctttcaga caggcggtga cttcgaccgg
    19141 cagactgagt tccgataatc ccaatctgca gaatatacca gtcagggatg aaaggggcag
    19201 ggaaatcagg aaagcgtttg tgccggaaga cggaaacctg tttttttcgg cagattattc
    19261 ccagattgaa ctgagactga tggctcatct gagcaaggat aaaggcatga ttgaggattt
    19321 cctctccggc agtgatattc atgccgcaac agcctcaaaa atattcgggg ttgaaatcgg
    19381 ggatgtgaca cgggaaatgc gaagcagggc aaaaacagcc aatttcggga tcatttacgg
    19441 tatttcatcc ttcggcctgt cagaaaggct tacgataggg cggaaagagg caaaagagct
    19501 tattgatggc tactttcagt catatcccgg ggtgaaaagg tatatgg
//
LOCUS       DAOOYG010000040        19358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2710_length_19358_cov_7.343729, whole genome shotgun sequence.
ACCESSION   DAOOYG010000040 DAOOYG010000000
VERSION     DAOOYG010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19358
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_19358_cov_7.343729"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK288_05265"
     CDS             <1..531
                     /locus_tag="PK288_05265"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPM18364.1"
                     /translation="LGDTLHLLINHWPSRRGGVLTGESSRSRIALMIRHVTDSLGSIS
                     CGKARVLIMGDFNCPPDDHIMQSLAGQSQGKANENGLSLVNLAVKAGKSVKGTYKYRG
                     AWETIDQMLASDHLLGCSKGLAADSLGFKVFSPEFLLCNDEKYTGSAPFSTYRGYRYQ
                     GGISDHLPVFLDLFLR"
     gene            complement(511..1947)
                     /locus_tag="PK288_05270"
     CDS             complement(511..1947)
                     /locus_tag="PK288_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM18365.1"
                     /translation="MNICLNDKIISALSEAVSSENIKAWVTGGYVRDCLLKREHGARD
                     VDIVVLGDGIESARKSARKLGPGIRVSVFRNFGTAMFRYNDFDIEFVGARKESYSRDS
                     RKPVVEAGTIEDDQKRRDFTINALAISINRETFGEFSDPFGGINDLKNKIIRTPLDPD
                     VTFSDDPLRMMRAVRFAAQLNFTIDPVTLKSIAKNAERINIVSAERIAGEFNRIIMSD
                     HPSKGFVLLEKTGLLKLIFPELDNLKGVEKKEGKAHKDNFLHSVKVLDNISSKTDNLW
                     LRWAALLHDIAKPQTKKYTPETGWSFHGHEYIGSKMIPGIFKRFKLPLNEKMKYVQKL
                     VDLHLRPMVLSQETVTDSAVRRLLFEAGDDIDDLMLLCEADITSKNEVTKKRHLENFK
                     LVRSKLVEIEAKDALRKFQPPVDGNEIIRTFGIKPGKEIGIIKNTIREAILDGIIPNE
                     HGAARELMLRKGRELGLTPADESSQEQI"
     gene            2098..2457
                     /gene="trxA"
                     /locus_tag="PK288_05275"
     CDS             2098..2457
                     /gene="trxA"
                     /locus_tag="PK288_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPM18366.1"
                     /translation="MVEHLTKESFLSKVFNYETNKEWKFEGERPCIIDFYADWCGPCK
                     MVAPILEELSREYDGKVDIFKVDTEAEQELAAVFGIRSIPSILFVPAEGQPQMAMGAL
                     PKDTFIKAFKDVLGVEK"
     gene            complement(2637..2834)
                     /gene="xseB"
                     /locus_tag="PK288_05280"
     CDS             complement(2637..2834)
                     /gene="xseB"
                     /locus_tag="PK288_05280"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPM18367.1"
                     /translation="MAKKEFSFSEAVKEIEKILRNIESGSLDVDILSAEVKKASELIR
                     ECQKKLKSTEDEINSIFKEMG"
     gene            complement(2890..4281)
                     /gene="xseA"
                     /locus_tag="PK288_05285"
     CDS             complement(2890..4281)
                     /gene="xseA"
                     /locus_tag="PK288_05285"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPM18368.1"
                     /translation="MSDDLKNKISLTELQLLIRDSLYLSLPDFYWVIAEISEIKENYS
                     GHCYLELVEKDPDDINIRARIRAVIWSSRYGFLKSFFEDLTGETLYSGIKILVKARIE
                     YHEIYGLSLVITDIDPSFTVGELAVKRQQILRKLEEEGVINMNKELGFPLVPQKIAVV
                     SSRNAAGYIDFLKHLANNPFGYGFHVRLFETPMQGTDTEPGVISALDKIAEEVSSFDV
                     VAIIRGGGSQTDLSWFDNYNIAFHITQFPLPVITGIGHEKDMSVTDLVAHIALKTPTA
                     VADFLIEGMRAADEHISDLSNRITEISRQVIEKNARLLESFGIKLIPLARILISELKE
                     QLSGVIIELVNSGKGFILKAGMLPAGHKARIMSASISLLGSQATELKNIKENLVNASR
                     MLLDNMKKKTESAENSLKILDPRNVLKRGYTITSLNGKILRSGNQLKTNDLITTRFSD
                     GSVKSRVEDQVKS"
     gene            complement(4278..5066)
                     /locus_tag="PK288_05290"
     CDS             complement(4278..5066)
                     /locus_tag="PK288_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18369.1"
                     /translation="MPLKITAAFLVFCFMAINAFQDDKSLNQTDSQGRKQGRWIKKYP
                     NGNIMYDGFFKDDHPTGEFKRYYENNVLKSLLVFSPDGNEAEAVLYYTNGFKASEGKY
                     FRQMKEGRWKFFSFFTKGLLISEEEYTNDKLHGWLIKYYPDSVVAEKQHYMAGIKDGS
                     WMKYHPDGSLHFNTTYSNGRLNGKFEAFFADGGRPEVTGQYKNDRKEGTWIIYNKDGS
                     IKFKTEYVAGVPKSREMDLYETNYIDSLENLKRNTEDPEKMQIR"
     gene            complement(5298..7211)
                     /gene="dnaK"
                     /locus_tag="PK288_05295"
     CDS             complement(5298..7211)
                     /gene="dnaK"
                     /locus_tag="PK288_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPM18370.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVIIPNSEGKRTTPSIVAFLD
                     NGERKVGDPAKRQAITNAKKTVYSIKRFMGETYDKVDEEIKRMTFPVVRGDNNTPRVK
                     IDNRMYSPQEISAMILQKMKKTAEDYLGQEVTDAVITVPAYFSDSQRQATKEAGEIAG
                     LNVKRIINEPTAASLAYGLDKKSQDLKIAVFDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDHMIIDWLAEEFLKDEGVDLRKDPMALQRLKEAAEKAKIELSSSTSTEIN
                     LPYIMPVDGIPKHLVKTLTRAHFEKLCDKLIQACIEPCQKALNDSGLKASEIDEVLLV
                     GGSTRIPAIQQLVEKFFGRVPSKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLS
                     LGIETMGGVMTRLIEANTTIPTKKMEVFTTAADSQPSVEIHVLQGERPIASGNKTIGR
                     FHLDGIPPAPRGVPQIEVTFDIDANGILHVTAKDRGTGKEQRIRIEASSGLSDDEIKR
                     MREEAKANAEADSKAKERADKINAADSMIFTTEKQLKEFGDKIPSDKKSAIENALSRL
                     KEAHKSQDLSEIDKALAELNAVWQTASEEMYKHAQASGASSQPGPETGHTDDGKARSG
                     NDEVTDVDFEEVK"
     gene            7565..8380
                     /gene="panB"
                     /locus_tag="PK288_05300"
     CDS             7565..8380
                     /gene="panB"
                     /locus_tag="PK288_05300"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPM18371.1"
                     /translation="MSNSSTARRITTHVLFEMKLKGEKIAMLTGYDYSIARILDEAGI
                     DIILVGDSASNVMAGYETTLPITLENMIYHAASVVRAVKRAMVVVDLPFGTYQGNSKE
                     ALASSIRIMKETGAHAVKLEGGYQVAESVERILSAGIPVMGHLGLMPQSIHKFGTYVV
                     RAKEQEEARRLLEDARILEKTGCFAVVLEKIPARLAEEVTNTLKIPVIGIGAGSKTDG
                     QVLVLHDMLGITKEFSPRFLRRYHNLYDEIKGAVQVYISDVRTLSFPNEKEQY"
     gene            8395..9072
                     /locus_tag="PK288_05305"
     CDS             8395..9072
                     /locus_tag="PK288_05305"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPM18372.1"
                     /translation="MEVLYEDNHLLAVNKKASDLSQGDASGDSSLDLQIKDYLSAKYN
                     KPGEVFLGVVHRLDRPVGGVILYARTSKALRRLNELFRNREVTKTYLAIVGNRPPEDK
                     ATITHFLRKNEKQNRANVFDRQVKGSQEASLTYRLAGRSDRYFLLEIELHSGRHHQIR
                     AQLSKIGCPVKGDLKYGSPRSNNDGSISLFARRLELIHPVRKEKIEICAPLPETDVWK
                     HFSGVEV"
     gene            9327..10973
                     /locus_tag="PK288_05310"
     CDS             9327..10973
                     /locus_tag="PK288_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM18373.1"
                     /translation="MSRNKFIGLIIMMLISLTGIIWVQIVWIRNAVNIRNENFNRTVF
                     ASLNDAANSIESNRRLGFFNDLMFGEPVSPLNFESGISGYLSVGSYSSSSSDGRSFNF
                     SVARQSVVSSVGSESRVSVKNSSVIRSDTTFFADTGTIMIRYPSGRTPAGAAREPDRY
                     PNNSQSIFVNRQEFLDWVRRRASQFQNMSDQMISEVYQWEKSMELDNNEIGAALQRAF
                     LFSGIETPFEFAVIKDGRIEENTTAKSARNDFLKSIYKVQLFPDNIISQNLILSVVFP
                     GRTNYVLRSMAWLLGGSLLFSFIILATFALSLFFIIRQKKISEMKSDFLNNMTHEFKT
                     PIATISLAADTITNPKIIRDESSIRHFIGMIKKENSRMNKKVETILQIASLDKKEINF
                     NYDNLSLHNIIERAAETIEIHLHQKKGKLKLQLDATDPVIYGDQEHLTNLVSNLLDNA
                     VKYSPEYPDITVLTYNTDGGIMMSVLDKGIGMTKSVQSRIFERFYRQGSGNVHDVKGF
                     GLGLNYVKAIIDAHRGSISVFSEPGKGSRFDIFLPSNWEN"
     gene            10973..11674
                     /locus_tag="PK288_05315"
     CDS             10973..11674
                     /locus_tag="PK288_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM18374.1"
                     /translation="MKMKLAKIFLVEDDLSFGSVLKSYLELNEYEVVWVDDGKNAIEH
                     FKQDVFDICILDVMLPHVDGFTLATEIRNINPAVPIVFLTAKKLKEDVLKGYGAGGDD
                     YITKPFDTDILIAKIRAILSRRDFQTGHKDIFEIGKFIFNAKLRTLTSGDEEKKLSPK
                     EAQLLELLALNPNELIGREMALKRIWGSDDYFTARSMDVYITKLRKFLSEDDRINIKN
                     VHGAGFQLIIRSDEG"
     gene            complement(11652..12152)
                     /locus_tag="PK288_05320"
     CDS             complement(11652..12152)
                     /locus_tag="PK288_05320"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPM18375.1"
                     /translation="MTKIKKITFKYVEHESFSDLESEDSRLITEAKRAAENAYAPYSG
                     FRVGAAVMLESGIIVHGSNVENAAFPSGICAERTALAYAVANHAGDRPSAIAIAAMNG
                     TVFTSAPVSPCGNCRQMIIEEEYRNKKEIRILLYGEEKILIIESAASLLPLQFDKGSL
                     SLHHSL"
     gene            12447..13364
                     /locus_tag="PK288_05325"
     CDS             12447..13364
                     /locus_tag="PK288_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HPM18376.1"
                     /translation="MQSYKDLAINEMKRTGVPASITLAQGMLESDYGRSTLARTANNH
                     FGIKCHNDWKGQSVRQHDDRRNECFRKYQKIEDSFYDHSDFLRSGSRYSFLFELNPLD
                     YKAWAHGLKKAGYATNPDYAHLLIRKIEENKLHVYDEMYTASAGRARIKAPPREPASS
                     VPAGTAEKPAAATTDETVTFAAVLARVPAIKEKNKIQYIIVAEGDTREKLENEFQLLR
                     WELPRYNELKEDFVPFPGQILYLQPKRDKAEAGNDFHTAAEGETMYSISQQYGIRLGD
                     LLEMNRMAPGTEPTAGQKIWLRSMKPAGG"
     gene            complement(13369..14325)
                     /gene="fmt"
                     /locus_tag="PK288_05330"
     CDS             complement(13369..14325)
                     /gene="fmt"
                     /locus_tag="PK288_05330"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPM18377.1"
                     /translation="MKDLRVVFMGTPGFAVASLGSILINEYNVIAVVTAPDKPAGRGR
                     VLSKSPVREFAESHCLHLLQPHNLKDPVFIRNLIDLNPDIFVVVAFRMLPEAVWKIPP
                     MGTVNLHASLLPQYRGSAPVNHAIINGESVTGLTTFLIDDKIDTGNILLREEVPILPN
                     ETAGSLHDRLMKYGARLIIRTLAAMAENKISPQPQSNFMIPGEILKTAPKIFPEQCIV
                     DWNSPPVKIHNLIRGLSPSPAARSEFIRGNNRVTYKIFESLPEEGEHDLVPGAIVSDG
                     KSFLKVACKGGFVRLFSIQAEGRKRLDIQEFLRGFRIDGFRS"
     gene            complement(14369..15601)
                     /locus_tag="PK288_05335"
     CDS             complement(14369..15601)
                     /locus_tag="PK288_05335"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPM18378.1"
                     /translation="MEQIKLNTIEEAIEDIKNGKLLIVVDDEDRENEGDFVCAAELIT
                     PEIVNFMATHGRGLICAALPEERCEELELDLMVGINTSLHETQFTVSVDLLGPETTTG
                     VSARDRALTIKALVDPKTKPSDLGRPGHIFPLKAKTKGVLRRAGHTEAVVDLTRLAGL
                     NPGGALVEIMNDDGSMARLPDLEMLKNKFNIKIVAIRDLIRYKLERDSIIEKGEKVKL
                     PTEYGEFEFIPFRQTSNGLEHAALVKGSWTRDEPVLVRLHSSCFTGDIFGSMRCDCGP
                     QLHKAMQMVEAEGKGVVIYLSQEGRGIGLFNKIKAYKLQDEGMDTVQANLKLGFGEDE
                     RDYGVGASIMRELGLGKVRLISNNPVKRAGLEGYGISIVETIPLVIQSNPYNEFYLRT
                     KASKMGHNLTCHKHIVKD"
     gene            complement(15588..17117)
                     /locus_tag="PK288_05340"
     CDS             complement(15588..17117)
                     /locus_tag="PK288_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPM18379.1"
                     /translation="MKKLDRFVLKSFIGPLILTFFIVLIILILQFLWMYVDELAGKGL
                     EFKILAELLFQFSLSFVPMALPLAILLASLMTFGNMGEYSELTALKSSGIPLKRIMRS
                     LIILVGIISVISFFFSNNVLPYSNKKARTLLYDIRRKRPDINLQAGTFNNDIDGFSIK
                     ITSKDPVTNRLDRLYIYDHRDNKGNVSVTFADSGYMRITQDETAMSMILYNGYSYNEM
                     PEKTDALQEKKYPFRKDYFREQTILVSLSGFDLERTEIDLFKSHSAMKNIAQLTHDID
                     SLNRRYNSRVESYYDEFNMNNLYTGRRRIITLYRHVREDSTRNKNAGPLIAGELFNTL
                     PDIDKKAALIRAINVVKDASNFLTSKNESLHSEVRYIKGYEVNWNKKFTLSFACLVFF
                     FIGAPLGAIIRKGGLGTPAVISVLFFVIWYVISLSGEKLVEEDLISTFAGMWASSYIL
                     LPIGLFLVYKASNDSVMMNVETYTAFLKKTKDYLYRIIITGTKRRPRVTTQNFFNDGT
                     N"
     gene            complement(17228..17611)
                     /locus_tag="PK288_05345"
     CDS             complement(17228..17611)
                     /locus_tag="PK288_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HPM18380.1"
                     /translation="MRLKYELEYTLNCSPKTLFPRLSTPEGLSEWFADDVTIDGDIYT
                     FNWNKSEIKARIVTLRENKLVRFEWADEPEEKESNYFEFKISVHDLTGDLALIITDFA
                     EPDEKEDAIYLWDTQISDLKRALGL"
     gene            17754..17827
                     /locus_tag="PK288_05350"
     tRNA            17754..17827
                     /locus_tag="PK288_05350"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17788..17790,aa:Met,seq:cat)
     gene            complement(18201..19109)
                     /locus_tag="PK288_05355"
     CDS             complement(18201..19109)
                     /locus_tag="PK288_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18381.1"
                     /translation="MKRLLFILLTISLSCCTSRESNKKSSAELPVPLYGITVDDSWTG
                     QTKLNQIIAAIKAMPVKPTVRIVMSNKIEPKEYITTFREIHKVAYVMASPVDSYYMES
                     YTTIDDYLKRFKESYQALAAYTDIWEVANEINGEGWIGDDRQFNADKMYAAFKYIKGK
                     KARAAVTGYETEPDSHEMSMFEWLNKYVPQDMKDNLDYMIVSYYEDDNHGFQPNWQQV
                     FNQLQTMFPHSKVGFSECGNTADNATTESKTQMALHYYTMPKYVSNYIGGYFWWTWVQ
                     DCVPHEGNTIWQTINNGMLVEKKNFE"
BASE COUNT         5128 a         4326 c         4205 g         5699 t
ORIGIN      
        1 ctcggggata ctctccatct attgatcaac cactggcctt caaggagggg aggtgtcctg
       61 accggggaat catcccggtc acggattgct ttgatgatca gacacgttac tgattcactg
      121 ggcagtatct catgcggtaa agcccgggtt ttaatcatgg gcgattttaa ttgccctccg
      181 gacgatcata taatgcaaag tcttgccggc caatctcagg gaaaggcaaa tgagaacggc
      241 ctgtctcttg tgaatcttgc tgtaaaagcc gggaaatcag ttaaaggaac atataaatac
      301 aggggggctt gggaaacaat tgaccagatg ctggcatcgg atcatttatt gggatgcagt
      361 aaaggtttgg ccgcggactc ccttggattt aaagtgttca gtcctgaatt cctgttgtgc
      421 aatgatgaaa aatataccgg ttcagcacct ttttcaacat acaggggcta ccggtatcag
      481 ggaggaataa gcgatcacct gccagtattt ctagatctgt tcctgcgatg attcatctgc
      541 aggagtcagt cccagctccc tgcctttcct gagcatgagc tctcttgctg caccatgctc
      601 gtttgggatt ataccatcga gaattgcctc cctgatggtg ttcttgataa taccaatttc
      661 ttttccaggc tttattccaa aggttctgat aatctcattt ccatccacag gtggctggaa
      721 tttcctgaga gcatccttcg cctcaatttc aacaagcttg cttcttacca atttgaaatt
      781 ttcaagatgc ctcttttttg taacttcatt tttggaagta atatcagctt cacaaagcag
      841 catcaggtca tcaatatcat caccagcctc aaaaagaagc ctcctgacag ctgaatcagt
      901 cactgtctcc tgcgataaaa ccatcgggcg gaggtgaaga tcgacaagtt tctgaacata
      961 tttcatcttc tcgtttagtg gaagcttgaa cctcttaaaa atgccgggta tcattttgct
     1021 tcctatatat tcatgaccat gaaatgacca gccggtttcg ggggtatatt tttttgtctg
     1081 gggcttggca atatcgtgca gcaatgctgc ccatcggagc cataaattat ccgtcttact
     1141 gctgatgttg tcaagcacct tgacggaatg cagaaagttg tccttgtgtg ccttcccttc
     1201 ctttttctca actcccttca gattgtcaag ttccggaaaa atcagtttca gaaggccggt
     1261 cttctcaagc aggacaaacc ctttcgacgg atgatcgctc atgattatcc tgttaaattc
     1321 cccggcaatc ctttcggccg atacaatatt tattctttct gcatttttcg ctatcgactt
     1381 cagagttaca ggatctattg tgaaattgag ctgtgcggca aaccttacgg ccctcatcat
     1441 ccgaagagga tcgtcggaga aagtgacatc agggtcaagc ggagtccgga taattttgtt
     1501 tttcaagtca tttatacctc cgaacggatc ggaaaattca ccgaatgtct ccctgtttat
     1561 gcttattgcc agagcattta ttgtaaaatc cctccttttc tgatcatctt cgattgtccc
     1621 ggcttccacc accggctttc ttgagtcgcg gctataggat tcctttcttg caccaacaaa
     1681 ttcaatatca aaatcattat agcggaacat cgctgttcca aaattcctga aaacgcttac
     1741 cctgatcccc ggcccgagct tcctcgcaga tttcctggct gattctattc catctcccag
     1801 aaccactatg tccacatccc ttgccccatg ctccctttta agaaggcagt cccttacata
     1861 accgcctgtg acccatgctt taatattttc tgatgaaact gcttcggaga gagcggaaat
     1921 aattttgtca tttagacata tgttcatata taaatatgac aatttaataa atattcatga
     1981 caaagttaca atttataggc aattatcagt aaattgacaa agtattgacc gggtggcaca
     2041 atgattgaag agggtaaaac agatttgatt ttacgatgtt ataatttaac ttaggatatg
     2101 gtagagcatt tgacaaaaga atcattttta agcaaggtgt ttaattatga aaccaataag
     2161 gaatggaagt ttgaaggcga acggccatgc ataattgatt tttatgccga ctggtgcgga
     2221 ccatgcaaaa tggtggcacc cattcttgaa gaattgtcca gggagtatga tggcaaggtt
     2281 gacatattta aggtcgacac ggaggctgaa caggaactgg ctgcagtttt cggaatcaga
     2341 agcattcctt cgatcctttt tgtcccggct gaaggacaac cgcagatggc aatgggcgcg
     2401 ctgccaaagg acactttcat taaagctttt aaggatgtcc tgggcgttga aaaatgaatt
     2461 gttgataaat aactccgatt ttgtttaaaa atattatcag aaaaatgtaa caatccacat
     2521 acatcctgcg ttatataacc gaaacagaag gtagtaacct ctcggttaca tttttttcat
     2581 ggatttaggt ttagggtagt aaggtagtaa atcaggactt cggtcctgat ttatttttat
     2641 cccatttcct tgaaaattga attaatttca tcttctgtgg atttcagctt tttctgacat
     2701 tcccttatca gctcggaagc tttttttact tcggcagaca gaatatctac gtccaggctt
     2761 ccgctttcaa tattgcgaag tattttttca atttccttaa ctgcttcgct gaacgaaaac
     2821 tccttttttg ccatattcag ttattttgaa aattcatgtt ttttcgattt gtgtcttaca
     2881 agttccgtgt catgatttca cctggtcttc aaccctgctt ttcacggatc cgtcgctgaa
     2941 ccgtgtggtg ataaggtcat ttgtctttaa ctggttgcct gacctgagga ttttgccgtt
     3001 caatgaggtg attgtatatc cgcgtttcaa aacatttcgc ggatcaagga tcttgagtga
     3061 attttccgca ctttcggtct ttttcttcat gttgtccaaa agcatcctgg aagcattgac
     3121 aagattttct tttatgtttt tcagctccgt tgcctgagat cccagcagcg atatcgaagc
     3181 agacatgatc ctggccttgt gtccggcggg cagcattccg gctttaagaa tgaatccctt
     3241 tcctgaattt acaagttcaa taatgacacc tgaaagttgt tccttcagtt ccgaaatcaa
     3301 aatccttgca agcggaatca gtttaatacc gaagctttcc aggagccgcg cgtttttttc
     3361 gataacttgt ctggatattt cagttatgcg gttgctgagg tctgaaatat gttcatccgc
     3421 tgccctcatt ccttcaatca ggaagtcagc aactgccgtt ggtgttttaa gtgcaatgtg
     3481 ggcaacaagg tctgtgaccg acatgtcttt ctcatggcct atgccggtaa taacaggaag
     3541 gggaaactgg gttatatgga atgctatatt gtaattgtca aaccagctta ggtccgtttg
     3601 cgacccgcca cccctgatga ttgccaccac atcgaatgat gacacttctt cggctatctt
     3661 gtcaagggca ctgatcactc caggttcggt gtcagtcccc tgcattggtg tttcaaaaag
     3721 ccggacatgg aaaccatatc caaacggatt attagcaaga tgtttcagga aatcaatata
     3781 tccggcagca ttccttgagg agacaacagc gatcttttgc ggaaccagcg ggaatccgag
     3841 ttccttgttc atattgataa caccctcctc ttccagtttc ctgagtattt gctgtcgttt
     3901 tactgcaagt tcaccaacgg taaaggatgg atcaatatct gtaattacca gacttagtcc
     3961 gtatatttca tgatattcga tcctggcctt taccaggatt ttgatccctg aataaagtgt
     4021 ttctccggta agatcttcga aaaatgattt caggaatcca tagcggctgc tccagattac
     4081 agccctgatt ctggccctga tattaatatc gtccggatcc ttttcaacaa gttcaaggta
     4141 gcagtgacct gaataattct cctttatttc ggatatttca gcaataaccc agtagaaatc
     4201 gggcagcgag aggtacaggg aatccctgat caacagctgt agttcagtca gggaaatctt
     4261 gtttttcaga tcgtcactca ccttatttgc attttttccg gatcttctgt atttcttttc
     4321 agattctcga gagaatcaat gtaatttgtt tcatacagat ccatttcacg gcttttagga
     4381 actcccgcaa cgtattccgt tttgaatttt atgcttccat ccttgttgta gattatccat
     4441 gtgccttcct tcctgtcatt tttatactgg ccggtaactt cgggcctgcc gccgtcagcg
     4501 aagaaagctt cgaattttcc gttgagcctg ccgttactgt aagtagtatt aaaatgcaat
     4561 gatccgtcgg gatgatattt catccagctg ccgtccttaa tgccggccat ataatgctgt
     4621 ttctcggcaa caacgctgtc aggatagtat ttaatcagcc agccatgcag tttatcattg
     4681 gtatattcct cctcacttat aagcaatcct ttcgtgaaga atgaaaagaa tttccatctc
     4741 ccttctttca tctgcctgaa atacttgcct tccgacgcct tgaatccgtt ggtataatac
     4801 agtacagcct cagcttcatt accatctggt gaaaagacaa gcaacgactt aaggacattg
     4861 ttctcatagt atcttttaaa ttcacctgtc gggtggtcat ccttgaaaaa gccgtcgtac
     4921 attatattgc cattgggata ttttttgatc cagcggcctt gttttcttcc ctggctgtcg
     4981 gtctgattca gtgacttatc gtcttggaaa gcattaattg ccataaaaca aaacaccagg
     5041 aaagcagctg taattttgag tggcatggaa aacacagaga ttaacagtta aaattacaaa
     5101 atttacaggt tccgggacgc cctgacttca gaataaatcg gggcctttca gtggaggtac
     5161 cgattattgc ggcagccgta cccttcatat tgatatgaat tttcaataat tataaagggc
     5221 cggttcagaa gccgctgtac ctcacggtca agcaactttt aaaccggccc cgttccctat
     5281 cttttcccct gaaaggttta ctttacctct tcgaaatcaa catcggttac ttcatcattt
     5341 cctgaccggg ctttcccgtc atcagtgtga cccgtttcgg gtcccggttg tgacgacgca
     5401 ccggaggcct gtgcatgctt gtacatttct tctgatgcgg tttgccatac tgcattaagt
     5461 tctgcaagag ccttgtcgat ttcagacaga tcctgtgact tgtgtgcctc cttcagcctt
     5521 gagagtgcat tctcgatagc actctttttg tcggagggga tcttgtcacc gaattcctta
     5581 agctgtttct cggttgtaaa gatcatgctg tcagcggcat ttattttatc ggcacgttct
     5641 tttgccttac tgtcagcttc ggcatttgcc tttgcttctt ccctcattct ttttatttca
     5701 tcatcgctga gccccgatga tgcttcgatc cttatgcgtt gttccttacc tgtacccctg
     5761 tccttggcag taacatgcaa aattccgttt gcgtcaatat cgaatgttac ttcaatctgc
     5821 gggactcccc ttggagcagg cgggataccg tcaagatgaa atcgtccgat cgttttgttg
     5881 ccagaagcga ttggcctttc cccctggaga acgtggatct caactgatgg ctgactgtct
     5941 gcagcagtgg taaatacctc catcttcttg gtgggtatgg tggtgtttgc ttcaatgagt
     6001 cttgtcatta ctcctcccat ggtttcaata ccaagtgaaa gcggagtgac gtcaagcagc
     6061 aatacatcct ttacttctcc ggtaagcaca ccgccctgga tggctgcccc gacagccacc
     6121 acctcatccg gattcacacc ctttgaaggt accctgccaa agaacttctc aacaagctgc
     6181 tgtattgcag gtatacgcgt agatcctccc acaaggagaa cttcatcaat ctcggaagct
     6241 ttcagccccg aatcattcaa tgctttctgg cacggttcaa tacatgcctg gataagcttg
     6301 tcgcatagtt tctcgaaatg tgcccttgta agtgttttta caagatgttt tggaattccg
     6361 tcaaccggca ttatgtaagg caggttgatc tctgtggatg ttgaacttga aagctcaatt
     6421 tttgctttct cagctgcttc cttcagcctt tgcagggcca ttgggtcctt cctgagatca
     6481 acgccctcat ccttgagaaa ttcctctgca agccagtcga ttatcatatg atcaaaatca
     6541 tccccgccaa gatgtgtatc gccattggtt gatttgacct caaatactcc atctccaagt
     6601 tcaagaatgg aaatgtcgaa tgtacctccg ccaagatcaa atacagcaat tttaagatcc
     6661 tgtgacttct tgtcaaggcc atatgccagt gaggctgcgg taggctcatt gattatcctt
     6721 ttaacgttca gacctgcaat ctcgccggct tctttggttg cctgcctctg tgaatcactg
     6781 aaatatgcag gtaccgttat aaccgcatcg gtaacctcct gcccgagata atcctctgcg
     6841 gtttttttca ttttctgaag gatcattgca gagatctcct gcggcgaata catcctgttg
     6901 tctatcttaa cccggggtgt attgttgtca cccctgacca cagggaaagt catccgcttt
     6961 atctcctcgt ccaccttatc gtaagtttca cccataaagc gtttgatcga gtaaaccgtc
     7021 tttttggcat ttgttatggc ctgacgcttg gccggatcac ccaccttgcg ttcgccattg
     7081 tcgagaaatg ccacaattga gggcgtcgtc cttttacctt cactgttggg aatgataacc
     7141 ggttcattgc cttccattac agctacgcat gaattcgttg ttcccaggtc aattcctata
     7201 atttttccca ttttatgttt gtttttaaaa agtattctgt tgatgactca tttattaaca
     7261 atgtttatgc cattcggaaa acatgacaaa acgacatgat ccggacgcca taactgtcaa
     7321 taaaaagata tattttccgt tgtaacgctt ccggggcaaa tatgtaactt tgctgagaat
     7381 gcccggaaca ccgggtatca tgattgatat ttaaatttac ctgacaacat tgctcaaaga
     7441 taggggagac gccggaggcc cggatgcagg gagaggaaac atccagcata gtttgctttc
     7501 agcgataccg gatactgcaa tttaaatccc cggcactgat taattaacaa taaatttcca
     7561 tattatgtcg aattccagta cagccaggcg gataacaact catgtgcttt ttgagatgaa
     7621 gcttaaaggt gaaaaaattg cgatgcttac gggctatgat tattccattg cgaggatcct
     7681 tgatgaagct ggtattgata ttatcctggt aggggattca gcttccaatg tcatggctgg
     7741 ttatgaaacc acactgccga taacgctcga gaacatgatc taccatgcag cttccgtagt
     7801 ccgcgccgtt aaaagggcca tggttgttgt cgatcttcct ttcgggactt accagggaaa
     7861 ttctaaggag gctcttgcct catcaataag gattatgaaa gaaactggcg cacatgcagt
     7921 taagcttgaa gggggttacc aggttgcgga atcggtagag cggatcctgt cagccggaat
     7981 acctgtaatg ggtcatctcg gactcatgcc ccagtcaata cataaatttg gtacttatgt
     8041 agtcagggca aaagaacagg aggaggcacg ccgcctcttg gaagatgcca ggattctcga
     8101 aaaaacgggt tgctttgccg tggtgcttga aaagatccct gccagactgg cggaagaagt
     8161 aaccaataca ttaaaaatac ctgttattgg aattggagcg ggatccaaaa cggacggcca
     8221 ggtgcttgta ctccacgata tgcttggaat cacaaaggaa ttttctccaa gatttctcag
     8281 aaggtatcat aatctctatg acgaaataaa gggagccgtt caggtttata tcagtgatgt
     8341 gagaactctt tcctttccaa atgagaaaga gcaatattag tcaaatgcaa tatgattgaa
     8401 gttctttatg aagataatca tctgcttgcg gtgaataaaa aggcatctga cctttctcag
     8461 ggcgatgcat caggggacag ttcccttgat ttacagataa aagattattt atcggcaaaa
     8521 tataataaac cgggcgaggt tttcctggga gtcgtacaca ggcttgacag gcctgttggt
     8581 ggagtgatcc tttatgcccg aacttccaag gcgctgcgca ggctgaatga attgttccgt
     8641 aacagggaag taacgaagac ctatcttgca atagtcggga accggcctcc cgaagacaag
     8701 gcaaccataa ctcattttct ccgtaagaat gaaaaacaga acagggcaaa cgttttcgac
     8761 aggcaggtaa aaggatcgca ggaggcatca cttacttaca ggctggccgg cagatcggat
     8821 cgctactttc tcctcgaaat agaacttcat tctggacgcc accatcagat cagggctcag
     8881 ctctcaaaga tcggatgccc ggttaaaggc gacctgaaat acggatctcc caggtccaac
     8941 aatgacggta gcatcagtct gtttgccagg cgacttgaac ttatccatcc cgttagaaaa
     9001 gagaaaattg agatatgtgc ccccctgcct gagactgacg tctggaaaca tttcagcgga
     9061 gttgaggtgt gatgcaccct catttctgac acaacgaaat gccacttgtt ggcttcatcc
     9121 ccattcttat attcctgcga aactaacaat gaaaattgat tgagccggga ataaaaggat
     9181 attttaatat ttttataact tgcttaataa gtatataacc aaaaaatccc aaacttgatc
     9241 cgaacctcgt taatatcagg ttaatttgag ttaatgaaac gttaaagcca tgttttgaac
     9301 gaaggcaatg atttaatttt gcaatcatga gccgtaacaa atttattggt ctgataataa
     9361 tgatgctgat atcacttacc ggcatcatat gggttcagat tgtatggatt cgaaatgcag
     9421 tcaatatccg gaacgagaat tttaacagaa cagtatttgc aagtctcaat gatgctgcca
     9481 attctataga atcaaacagg cggctgggtt tcttcaacga cttaatgttc ggcgaaccgg
     9541 tttcaccgct gaattttgaa agcggaatca gcggctatct tagtgtggga agctactcat
     9601 cctcatcctc cgacgggagg agttttaatt tcagcgttgc cagacaatca gtggtcagtt
     9661 ccgttggctc tgaatccagg gtaagcgtaa aaaattcatc ggtcatacgc tctgatacta
     9721 cttttttcgc cgacaccggt acaataatga tcaggtatcc gtccggccgg acgcctgcag
     9781 gtgctgccag ggaacctgac agatatccca ataattcaca gtcaatattt gttaaccgcc
     9841 aggaattcct cgattgggta agaaggaggg caagccagtt ccagaacatg agtgatcaga
     9901 tgatatcgga ggtttatcag tgggaaaaaa gcatggaact cgacaacaat gagataggag
     9961 ctgcattgca gagggcattc ctgttttcag gcatcgaaac tccttttgaa tttgcagtta
    10021 tcaaagatgg caggattgag gaaaatacta ccgcaaaatc ggccagaaac gattttctga
    10081 aaagcattta taaggttcag ctctttcccg acaatataat aagccagaat ctgatccttt
    10141 cggtggtatt tcctgggcgc accaattatg ttctcaggtc tatggcctgg ctgctgggcg
    10201 gatccctttt attttcgttc ataattctgg ctacttttgc actgagtctt ttcttcataa
    10261 taagacaaaa aaagatttcg gaaatgaaat ccgacttttt gaacaacatg acccacgagt
    10321 ttaaaacccc gattgccaca atttcactgg ctgcagatac aataacaaac ccgaagataa
    10381 tcagggatga atcgagtatt cgtcatttca taggaatgat aaagaaggaa aacagcagga
    10441 tgaacaaaaa ggtggaaacc atcctgcaga ttgcttcact cgacaagaaa gaaattaatt
    10501 tcaactatga taacctgtca ctgcataaca taattgaaag ggcagccgag acaatcgaaa
    10561 tacacctgca tcagaaaaag ggaaaactga agcttcagct tgatgcaact gacccggtaa
    10621 tttatggtga tcaggagcat cttacaaatc ttgtcagcaa ccttctcgac aacgctgtaa
    10681 aatattctcc cgaatatccc gatatcactg tcctgacata taataccgat ggcggaataa
    10741 tgatgtcggt gctggacaag ggaataggta tgacaaaaag cgttcaaagc aggatttttg
    10801 aaagatttta ccgtcagggt tcggggaatg tacatgatgt taagggtttt ggcctgggac
    10861 tgaattatgt aaaagcgatt atcgatgcac acagggggtc aattagcgtt ttcagtgaac
    10921 ctggtaaagg cagcaggttc gatatattcc tgccatcaaa ctgggaaaat taataaaaat
    10981 gaaattagca aaaatctttc ttgttgagga tgacctgagt ttcggctcgg tgttgaaatc
    11041 ctatcttgaa ctgaatgaat acgaggttgt atgggtggat gacgggaaaa atgcgattga
    11101 acatttcaag caggatgtgt tcgatatctg catactcgat gtgatgctgc cccacgtgga
    11161 tggttttacc ctggcaaccg agataagaaa tatcaaccct gccgttccaa tagtctttct
    11221 caccgcaaaa aagttaaagg aggatgttct taaaggctat ggagcaggag gggatgatta
    11281 cattactaaa cctttcgaca ccgatatcct gattgcaaag atacgggcaa tcctttcaag
    11341 gcgcgatttt cagacgggcc ataaagatat attcgagatt ggcaagttta ttttcaatgc
    11401 caagctcagg acacttacat ccggagacga ggaaaaaaag ctgtctccca aggaagctca
    11461 gcttctcgaa cttctggcac tgaatcccaa tgagctgata ggtagggaaa tggccctgaa
    11521 aagaatatgg ggttccgatg attactttac tgccagaagc atggatgtat acattacaaa
    11581 gctcaggaaa tttttatcgg aggatgacag gatcaatatt aaaaatgttc atggagccgg
    11641 tttccagctg attataagga gtgatgaagg ctgaggcttc ccttgtcaaa ttggagcggg
    11701 aggagcgagg cggcactttc aataatgagt atcttttctt ccccgtaaag taaaatcctg
    11761 atctctttct tatttcggta ttcttcctca atgatcatct gtctgcagtt accgcagggt
    11821 gaaacaggag ctgaggtaaa tacagttcca ttcattgcag ctatggcaat tgccgatggc
    11881 ctgtcgccgg catgatttgc caccgcataa gccagggcag tcctctcggc acagattcct
    11941 gacgggaatg ccgcattttc aacattagat ccgtgtacga taataccgga ttcaagcatg
    12001 acagctgcac caaccctgaa gccggagtat ggagcatagg cgttttctgc agcccttttt
    12061 gcctctgtta taagccggga gtcttcactt tcaagatcgc tgaaactttc atgttccacg
    12121 tatttgaaag tgattttttt aatttttgtc atagtacagc ctgtcgggat cagatgataa
    12181 aattaacaaa attcgtgtta aacagggtgt aaattcattt ctttcaatga ttttaccaag
    12241 actgtcccag actgcattcc ctttgaattc tttttgtatt tttgcctgaa acagaaaaaa
    12301 ggttgaaatt gcgcatacca tttattttcc tgttagttgc gctgcttttc ctgctttcgt
    12361 gcagggccgc aaggcagcct gtatcctcac acgtcgtcgt ttccggcacc tctcatgccc
    12421 ctgttactgc ccctgccgct gcctacatcc agtcctacaa ggatctggct ataaatgaaa
    12481 tgaaaagaac cggagtacct gcaagcatta ccctggcaca gggtatgctt gaatcggatt
    12541 acggacggag cactcttgcc aggacagcca acaatcattt cgggataaaa tgccataatg
    12601 actggaaagg tcagtctgta cggcaacatg atgacaggag gaatgaatgc ttcaggaaat
    12661 accagaagat agaggattca ttctatgatc attctgactt tttaagatcg ggttcccgct
    12721 acagttttct tttcgaactg aatcccctgg attacaaagc ctgggcgcat ggactaaaaa
    12781 aggctggtta cgctactaac cccgattatg cacatttact catcaggaaa attgaggaga
    12841 ataaacttca tgtttatgac gaaatgtata ctgcctcagc cggcagggcc agaataaagg
    12901 ctcctccgag ggagccggcc tcatccgtcc cggcaggaac tgcggaaaaa ccggcagctg
    12961 ctaccacaga tgaaactgtt acttttgccg ctgttctggc aagggttccc gctattaagg
    13021 aaaaaaacaa gattcaatat attattgtag ctgaaggcga tacccgtgag aagcttgaaa
    13081 acgaatttca gcttcttcga tgggaactgc caagatataa tgaacttaag gaggattttg
    13141 tcccgtttcc agggcagatc ctctaccttc agccaaaaag ggacaaggct gaggccggta
    13201 atgattttca cactgccgca gaaggggaaa ccatgtattc aatttcacag cagtatggta
    13261 taagactggg cgatctgctt gaaatgaaca ggatggcacc gggcacggaa cccacagccg
    13321 gccagaagat atggctaagg agcatgaagc cggcgggagg atgagttttc agcttctgaa
    13381 tccgtcaatc ctgaagccgc gcaggaattc ctgaatatca agccgttttc ttccttcagc
    13441 ctggatgctg aataatctta caaatcctcc cttacaggca accttaagga aacttttacc
    13501 atcagagacg atcgcaccgg gtacaagatc gtgttctcct tcctcgggca aactttcaaa
    13561 tatcttgtat gttaccctat tatttcccct tatgaattct gatcttgcag cgggactcgg
    13621 agagagcccc ctgatcaggt tgtgaatttt tacaggagga ctgttccagt caacaataca
    13681 ttgttcggga aagattttag gagccgtttt gagaatttcc ccgggaatca tgaagtttga
    13741 ctggggttgc gggctgattt tattttcagc cattgctgcg agggtcctga tgatgagcct
    13801 tgcaccgtat ttcatgagcc tgtcgtgaag gctgcctgca gtttcattag ggagaatggg
    13861 cacttcttct cttagaagaa tattccccgt atctatctta tcgtcgataa ggaaagttgt
    13921 cagaccggta actgattcac cattgataat ggcatggtta acaggtgccg atccccggta
    13981 ttgcggaagc agcgatgcat gaaggttcac agtgcccatt ggcggtattt tccatactgc
    14041 ttccggtagc attctgaaag ctacgacaac aaagatatcg ggattcaggt caatcagatt
    14101 cctgatgaat acgggatctt taagattatg aggctgaagt agatgaagac aatgtgattc
    14161 agcaaattcc ctgaccgggg atttggacaa aacccggcct cttccggcag gcttgtcggg
    14221 agccgtcaca acggcaatca cattgtattc atttatgaga attgagccaa gagaagcaac
    14281 tgcaaacccg ggagttccca taaaaactac cctgagatct ttcatagact gtagacattc
    14341 cttgtgtcat acaaatctac taaaaatatc aatccttgac aatatgcttg tggcaggtca
    14401 ggttatgccc cattttgctg gctttggttc tcagataaaa ctcattgtat gggttggatt
    14461 gtatcacgag agggattgtt tcaacaatgc tgataccata accttcgaga ccggcccttt
    14521 taaccggatt attcgatatg agccttacct ttccgaggcc cagttccctc atgatgctgg
    14581 ccccgacacc atagtccctt tcgtcttcgc caaatccgag cttgagattt gcctgtacgg
    14641 tatccattcc ttcatcctga agcttgtacg ccttgatctt gttgaaaagt ccgattccac
    14701 ggccctcctg gctaagatat atcacaacac cctttccttc ggcctccacc atctgcattg
    14761 ccttgtggag ctgcggtccg cagtcgcacc gcattgaacc gaagatatcg ccagtgaaac
    14821 acgaggaatg gagccttaca agcactggct catcccgagt ccatgatccc ttcacaaggg
    14881 ccgcatgttc cagtccgttg cttgtctgcc tgaaagggat gaattcaaat tctccatatt
    14941 cggtgggaag tttgaccttt tcaccttttt caatgatgga atccctttcc agtttatatc
    15001 tgatcagatc cctgatggcc actattttga tattgaattt atttttcagc atttccaggt
    15061 cgggcagccg tgccatcgag ccgtcatcat tcattatttc cacaagtgcg ccgcccgggt
    15121 ttaatccggc cagcctggtc aggtcgacaa ccgcttccgt gtgaccggct ctcctgagta
    15181 cccccttggt tttcgccttt aacgggaaaa tatgaccggg ccgcccgaga tccgagggtt
    15241 ttgttttggg atcaaccagt gcctttatgg taagtgcccg gtccctggct gaaacgcctg
    15301 tggtcgtttc aggaccgagg agatcgaccg atacggtaaa ctgtgtttca tgaagcgacg
    15361 tgttaattcc taccatcaga tcgagctcaa gttcttcgca tctctcttcc ggcagtgctg
    15421 cacatatgag cccgcgtcca tgcgtggcca tgaaattcac aatttcaggt gtaatgagct
    15481 cggctgcaca aacaaaatct ccttcattct ccctgtcctc atcatccaca actatcagca
    15541 gcttcccgtt ttttatgtct tcaatagcct cttcaattgt attaagctta atttgttcca
    15601 tcattaaaga aattttgagt tgttacccgg ggcctccttt ttgttccggt gattatgatc
    15661 ctgtacaggt aatcctttgt ctttttaagg aaggccgtat atgtttccac attcatcatt
    15721 actgaatcgt ttgatgcctt gtatacaaga aacagaccaa tcggaagaag aatgtatgaa
    15781 gaggcccaca ttcctgcgaa cgtgcttatc aggtcctctt cgaccagctt ttctccggaa
    15841 agtgaaatga cgtaccatat cacgaaaaaa agaacggaga taactgcggg ggttccaagg
    15901 cctccttttc ttattattgc accaagaggc gctccgataa agaaaaaaac caggcatgcg
    15961 aacgaaaggg taaatttttt gttccagttc acctcgtagc ctttgatata tctaacctca
    16021 gagtgcagcg attcgttctt cgaggtaagg aaattgcttg cgtccttcac tacattaatg
    16081 gccctaatca gtgcagcttt cttatctatg tcgggaagtg tattgaataa ttctccggca
    16141 attaatggac cggcattttt gtttcgggtt gagtcttccc tgacatggcg gtacagggta
    16201 ataatccttc ttcttcccgt gtaaaggtta ttcatgttga attcatcata atagctttcg
    16261 acccgggagt tgtatctccg gttaagcgaa tctatgtcgt gtgtaagctg agctatgttt
    16321 ttcattgccg aatgcgactt gaaaagatct atttcggtgc gttccaggtc aaatcccgaa
    16381 agtgaaacaa gaattgtctg ttcccggaaa tagtctttcc tgaatggata tttcttttcc
    16441 tgcagggcat cagtcttttc aggcatctcg ttataggagt aaccgttgta gaggatcatg
    16501 ctcatagccg tctcatcctg cgtaattctc atgtaacctg aatctgcaaa ggtgacggat
    16561 acgtttccct tgttgtcacg gtggtcgtag atataaagcc ggtcgagccg gttggttacc
    16621 ggatccttcg atgttatctt aatgctgaaa ccgtcaatgt cgttgttgaa ggtacctgcc
    16681 tgaaggttga tatcgggccg tttgcggcga atgtcgtaga gaagggttct ggccttcttg
    16741 ttggaatacg ggagcacatt attggaaaag aagaaagaaa tcacagagat aatcccgaca
    16801 aggataatca gtgacctcat gattcttttc agcggtatcc ccgatgactt gagggcagtc
    16861 agctcactgt attcacccat gttcccgaaa gtcatgagtg aggcaagcag gattgccagg
    16921 ggcagggcca tgggaacgaa cgacagggag aactgaaaaa ggagttcggc aagtatttta
    16981 aattcgagtc cttttcctgc aagttcatca acatacatcc acaggaattg aagtataagg
    17041 atgataagga ctatgaaaaa agtaaggatc agtggtccta taaatgattt taaaacgaat
    17101 ctgtcaagtt tcttcatagc cttaattttc aagatgcaaa attacttgat tttttgcaat
    17161 aacctgcagt caggctttgc caattatgaa taacacaggc agtctggaag gaaatgcatt
    17221 tttaatatca gagcccgagg gctctcttga gatctgatat ctgggtgtcc cacagataaa
    17281 tggcgtcttc cttctcgtcg ggttcggcga aatcggtgat tatcagggcc aaatcacccg
    17341 taagatcatg aactgatatt ttaaattcaa aataattgga ttccttttct tccggttcat
    17401 ctgcccattc gaatctgaca agtttgtttt ccctcaatgt cacaatccgg gctttgattt
    17461 cgctcttgtt ccagttaaag gtataaatgt ccccgtcaat tgttacgtca tctgcgaacc
    17521 attcactgag tccttcaggg gtgctgagcc gtggaaacaa tgtttttggt gagcagttca
    17581 gagtatattc cagttcgtac ttcaacctca ttttattagc agcaattagc ccttgcattc
    17641 tgcaatatag aaaaaaatat caaaagaaaa aatcatattg gaattttgat ggcaactcct
    17701 tatatttgca ccctcaaaaa gtacctccct cgctgaagct gctgaggtca aatggcgaga
    17761 tagctcagat ggttagagcg catgattcat aatcatgagg tcgggggttc aattccccct
    17821 ctcgctacaa tcaaaagccc tgtgaaagcc gaaggctgac acagggcttt tttgtttgaa
    17881 gcgcgtgagc ttgctcacag ggttgaaaaa ctaaaaagcc cgtagtcagg ccccagcctg
    17941 acagggtttt tggttgttaa gctccgccct gtgaatcgcc tccggacaat tacccctctc
    18001 tctacacgga aaatcaacca cttccatagt caaacatgga ggtgatattt gttttgtgcc
    18061 aggtttttat gaaaagatta attgagagat gatatctggt atcttagaaa tggagaccat
    18121 ttcattcacc agtgaggttt tatgcaatag ttcatccaag tatttatctt caatttctca
    18181 aaatagtgcc atatcagatt ttactcgaaa ttcttcttct caactaacat accgttgttg
    18241 atagtctgcc atattgtatt gccttcgtgt gggacgcaat cctgcaccca ggtccaccag
    18301 aaatagccgc ctatataatt tgatacatat tttggcatgg tgtagtaatg taaagccatt
    18361 tgggttttgc tttcggtagt ggcattatct gccgtgttgc cacattcgct gaaaccaacc
    18421 tttgaatggg ggaacattgt ctgaagttgg ttaaaaacct gctgccaatt gggctgaaat
    18481 ccgtggttgt catcttcata atagctgacg atcatataat caagattatc cttcatatcc
    18541 tgagggacat atttattcaa ccattcaaac atgctcattt catggctgtc aggttctgtc
    18601 tcgtagcctg taactgctgc tctggccttc tttcctttaa tatatttaaa agccgcgtac
    18661 attttatcgg cattaaattg ccggtcatcg ccaatccagc cttcaccatt gatttcattg
    18721 gctacttccc agatgtcagt ataagcagca agtgcttgat atgattcctt aaaccgcttc
    18781 aaataatcgt caatagttgt atatgattcc atgtaatatg aatcaaccgg gctggccata
    18841 acataagcaa ctttgtggat ttccctgaag gtggtaatat actcctttgg ttcaattttg
    18901 tttgacatga caatgcggac ggtcggcttt acaggcatgg ctttgatggc agctatgatc
    18961 tggttaagtt tggtttggcc tgtccaactg tcatcaacag ttatcccgta taaggggacc
    19021 ggtaattcag ctgatgattt tttgttgctt tcccggcttg tgcagcatga aagggaaata
    19081 gttaaaagaa tgaacagtaa tcttttcatg atattaatag ttatagttaa tgttacctcg
    19141 cgaattctat tagcttcctt tcagtgtaat tgagaaggcc tgaaattaac ctgcttataa
    19201 atttatgcgg tatttaagat tttgtcaaca tctcggttat atgcctcctg acttttggtt
    19261 ctgttccttt tgcaataagg gtattacggc cttcatagcc gccttcatca atcatcttgt
    19321 cggtgggcat atatgataag gtacccccag taaggctt
//
LOCUS       DAOOYG010000041        19162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2759_length_19162_cov_7.514680, whole genome shotgun sequence.
ACCESSION   DAOOYG010000041 DAOOYG010000000
VERSION     DAOOYG010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19162
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2759_length_19162_cov_7.514680"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2113
                     /locus_tag="PK288_05360"
     CDS             <1..2113
                     /locus_tag="PK288_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPM18382.1"
                     /translation="IAVMLKQQGKVFRIEKQVHSYPHCWRTDKPVLYYPMDAWFIRTT
                     AFREKMIELNNTINWKPVSTGTGRFGKWLENLVDWNLSRSRYWGTPLPIWVSGDRTEE
                     KCIGSAAELKEEIEKSVKAGFMTANPLKDFIEGDNSSENYGKFDLHRPFVDEIILVSP
                     TGSKMFREPDLIDVWFDSGAMPYAQAHYPFENKENFSDMFPADFIAEGVDQTRGWFFT
                     LHAIASMISGSVAFRNIISNGLVLDKNGNKMSKRLGNAVDPFTAIEEYGSDPLRWYML
                     TNAQPWDNLKFDIEGVDEVRRKFFGTLYNTYSFFALYANIDKFRYTEKEIPVGERPEI
                     DRWIISLLNSLVREVGKCFDDYDLTRAGRAIQDFVTENLSNWYVRLNRKRFWGGDYDK
                     DKLAAYQTLYRCLETVSRLAAPIAPFYTDRLFTDLNGTTGHHQEDSVHLTSFPVPDHD
                     LIDEELEEKMEIAQKISSMILGLRRKVNIKVRQPLARIMIPVSDEEFRKKFEAVRDLI
                     LAEVNVKNVEYIDDTSSVLIKKAKPDFRLLGPRYGRLMKQIGNIVSGLTQEEITSFEN
                     TGALVFEIDGQKVELTTDDVEIISEDIPGWQVANDGRLTVALDVTVTDELRYEGIARE
                     FVNRIQNIRKESGFDVTDKITVLIEDMDVIGAAVRRHSSYIGSQTLATRVDVVRDFTN
                     GDVRDVEINDIQVRISVSRNC"
     gene            complement(2129..2506)
                     /locus_tag="PK288_05365"
     CDS             complement(2129..2506)
                     /locus_tag="PK288_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPM18383.1"
                     /translation="MAEKTRYSDEELEEFREIILRKLDKAKKDYEILKSSITHEESND
                     TMDTSPTFKVLEEGATTLSKEEAGRLAQRQQKFIQHLQAALVRIENKTYGICRETGKL
                     IAKERLRAVPHATLSIDAKMRQK"
     gene            2684..3244
                     /locus_tag="PK288_05370"
     CDS             2684..3244
                     /locus_tag="PK288_05370"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPM18384.1"
                     /translation="MTTEGDSKKALNRLIRKTPEPRWYAVYTNPRAEKQVRDRLLEAN
                     VEVFLPLQKTYRMWSDRKKLIEKPLLSSYVFVRVSPAEFPRVYQTRGIVRFVTFEGQP
                     ASIPQNQIDNLRLLINSDTEIEVTSGKFERGDNVEVVRGALVGLTGELIEIGSQKRVI
                     IRIDRLDQNLILKIPLTFLRKTGEKN"
     gene            complement(3280..4407)
                     /locus_tag="PK288_05375"
     CDS             complement(3280..4407)
                     /locus_tag="PK288_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPM18385.1"
                     /translation="MKKNSRIYVAGHTGLVGSALTLTLREKGYDNLLLRTAEELDLTD
                     QRATDSFFRDQKPEYVFLAAAKVGGIVANNTLRADFIYINLMIQNNVISSAWKHGVKK
                     LLFLGSTCIYPKNAPQPLREEYLLTSQLEYTNEPYAIAKIAGLKMCESFNIQYNTNFI
                     AVMPTNLYGYGDNYNLEGSHVLPALLRKFHLGRCLETGDMDAIRRDLDRYPIEGISGS
                     SDDGSITGILRKYGVKLTERSGTGRHEVAVSVWGTGTPLREFMNSKDMAEACVFIMEN
                     VDADDIQAMHRKGNPDPEFHPPHFLNIGTGEEFSIKDLTYKIKALTGFTGEIVFDTSK
                     PDGTMRKTIDITALTGLGYKYRIPLDEGLKDTYSMYLRYKS"
     gene            complement(4498..5655)
                     /gene="gmd"
                     /locus_tag="PK288_05380"
     CDS             complement(4498..5655)
                     /gene="gmd"
                     /locus_tag="PK288_05380"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPM18386.1"
                     /translation="MSKTALITGVTGQDGAYLAEYLLKKDYIVHGIKRRSSLFNTERI
                     DHLYQDPQAEKRNFILHYGDLTDSMNITKLIQECHPDEIYNLAAMSHVKVSFDTPEYT
                     ANADGIGTLRILEAVRLLGLIRKTRIYQASTSELYGLVQEVPQTEKTPFYPRSPYGVA
                     KLYAYWITVNYREAYNMHASNGILFNHESPIRGETFVTRKISRAMARIALGMQNCLYM
                     GNLSSRRDWGHAKDYIKAMYLILQQDQPDDYVIATGVTTSIREFIIKTFQAAGMEVVF
                     SGEKENEVGKITAIDEKLFATMVGEEFSEPIRSRIKNNPIVVKVDKNYFRPTEVDLLI
                     GDSTKARQKLGWSPRFDLDGLIQDMINSDINLMKKEAHIKNGGFRTLNYFE"
     gene            complement(5823..6980)
                     /locus_tag="PK288_05385"
     CDS             complement(5823..6980)
                     /locus_tag="PK288_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HPM18387.1"
                     /translation="MSIKKKVLVSGCFDMLHSGHIAFLQEASEYGELYIGLGSDKTIR
                     ELKGRETVNSEAERVYILSALSCVHKAFVNKGSGLLDFAEDLKELSPDIFIVNEDGHS
                     PEKEKLCRDHGIEYKVLKRIPHGNLPARSTTALRTIQPMPYRIDLAGTWIDQPYVSKY
                     HPGAAITASLEPTIEFNERSGMATSTRKKAIELWNYHLPLEKPEKLARLLFRYDNDPG
                     TTEVSGSQDSIGITMPGITWFYYDRGEYWPSKFETISDLNTIKWLEDRLYMLTLWPRP
                     NGYNVLSDTNINAENVKRLADAAGLAWEGLVSRDFGRFTKGFLESFRSQVRMFPKMMN
                     ADIQRVIDQYRETASAWKLSGAGGGGYLILISEQEIPNAFRIKIRMKDFGM"
     gene            7288..7662
                     /locus_tag="PK288_05390"
     CDS             7288..7662
                     /locus_tag="PK288_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HPM18388.1"
                     /translation="MKEYLKTRNHTTFKRYCKFLRLQDDPVLIEKYRQIHAPGMVWPD
                     ITGDMKEAGILDMEIYIHGNNAFMIMETVPDFDHEKAMRALASKPRQVEWENYVSRFQ
                     HAEGRSDTPEKWEIMERIFLLD"
     gene            complement(7836..8318)
                     /locus_tag="PK288_05395"
     CDS             complement(7836..8318)
                     /locus_tag="PK288_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPM18389.1"
                     /translation="MKTIKIKAMDMKQRLDYSRESDGALKGLLELERYVHGSGLERTL
                     YELVKTRASQINGCAYCIDMHTKDARKAGETEQRLYTLSAWRETPFYTPRERAALEWT
                     EALTLISGNDVPDPLYEAVREHFTEKEIIALTMAIIAINGWNRLAISFRTPPGSYKTQ
                     "
     gene            complement(8315..8614)
                     /locus_tag="PK288_05400"
     CDS             complement(8315..8614)
                     /locus_tag="PK288_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18390.1"
                     /translation="MYDKYSPVDPDFYDIIEDIRKRNLSVLIHYFGPAKELNDARGTI
                     QGVTGNSDHEEFLALDSGSKVRLDRIITLNGRPGPAFDEYDSYSLACLDCTAGMD"
     gene            complement(8920..9729)
                     /locus_tag="PK288_05405"
     CDS             complement(8920..9729)
                     /locus_tag="PK288_05405"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPM18391.1"
                     /translation="MKKMILLTICMLIPSTFIRAQVSIIAHRGASYLAPENTIASAKL
                     AWKHGADAVECDIWLSKDNKIIVSHDGNTKRTTGHDHKISETNSDVLRKLDAGSWKDP
                     KYKGQKLPFLHELIKTVPAGKEIVIEIKCGPEVLPLLKETIAGYAAKRKFTFICFDLP
                     TITATREAFPQYPCYWLCDNPSLLEENFHKVKEAGLQGFSLNYRLITREIVDRASEMN
                     LEVFAWTVNDRDEAHRLISLGVMGITTDRPQWLIEQLFARSPDSHSRPSGK"
     gene            10207..11541
                     /locus_tag="PK288_05410"
     CDS             10207..11541
                     /locus_tag="PK288_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPM18392.1"
                     /translation="MAEKDLIVTMRQFFPYIGICIMLVLFTGVMSRAGEDSGRGPVVV
                     ISLDGSINPATQEYINSGIRNAREKNAECLVIRLNTPGGLLKSTRVIVTDILDSAVPV
                     VVFVYPGGSQAASAGVFITLAAHIAAMAPGTNIGAAHPVTMQGQQDSIMNEKATNDAA
                     AFIRTISEKRERNIRWAEDAVRKSLSITESEALKEGVIDLVAVSVEDLLEKIDKREVV
                     LSSGKKVLDTKGAEVINMEMNFKQKILSLLSDPNIAYILLMIGIYGIMFELYNPGAIF
                     PGVIGGISIILAFYSLHTLPVNYAGLALIIFAVILFLAEIKVVSHGLLAIGGVISLIL
                     GSIMLINTETTMEVIKISWQVILIIVILTAAFFLFAISFGIKAQGRKPTTGIEGMIGE
                     TGEAITNLDPEGQISVRGEVWNAESLEGPVSKGTRVKINGVSNLKLLIRKTT"
     gene            11565..12326
                     /locus_tag="PK288_05415"
     CDS             11565..12326
                     /locus_tag="PK288_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPM18393.1"
                     /translation="MEQTSYTLLITGIIFLLIILASAVRILREYQRAVIFRLGRLIKV
                     KGPGLILLIPVIDRMVRVSLRTVVLDVPPQDVITKDNVSIKVNAVVYFRVIQPEKAIV
                     EVENYLFATSQLSQTTLRSILGQSELDELLSHRDKINHELAKIIDSQTEPWGIKVSMV
                     EVKHIDLPQEMQRAMAKQAEAERERRAKVIHAEGEFQASQKLSDAASIISRNPSALQL
                     RFLQTLTEVASEKSSTIVFPVPLDLISAFMPSEKK"
     gene            12539..13552
                     /locus_tag="PK288_05420"
     CDS             12539..13552
                     /locus_tag="PK288_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM18394.1"
                     /translation="MAKYLITGTAGFIGYHIAKRLIGHGNEIVGIDNVNDYYDVNLKF
                     ARLEDTGIMRDEVSGKELIRSRKSGEYRFKKMNLEDNDSLLALFRDEKFDYVIHLAAQ
                     AGVRYSIENPGVYISSNITGTFNILEACRHYPVKFLMYASSSSVYGNSEKVPFSEDDN
                     VDSPVSLYAATKKSNELMAASYNHLYGIESTGLRFFTVYGPWGRPDMAIFKFTRAILN
                     NEPLQVFAGGRLLRDFTYIDDIVNGMTAILKQQTVSAAPSVMNIGNNSPVTVSSLISE
                     LEECLGRKAIINYLPMQQGDVKATYASISRIGEYCGFAPRTDLKAGLSKFCRWYLDYY
                     GIR"
     gene            complement(13694..15247)
                     /locus_tag="PK288_05425"
     CDS             complement(13694..15247)
                     /locus_tag="PK288_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPM18395.1"
                     /translation="MKAVKFRTFTLIADIVILAISFLIAASFKPSGLTGYLPTHYIFL
                     AGLSLIWIIISLLNGKMHRGRIVNFTTLFSRVLTSNFIALAIIALLMYAFREYSFSRT
                     VVLGTACIATFLELLIGTIYIEYKKAVIQDYQNYDEYKATRKKSEEELVEKTNGNGNG
                     NIHINENDINPRVIRAIEREAGAEMARAIMNIAGARLNKKTAVVSTTTIFNITNLPEE
                     QYDYIINLHRFNDIKKLNYFLDEVNFKLPVKGYFLGCVETKDQRKKRLLKKYPPVLNY
                     FYYSADFVVKRILPKIKFTRKLYHLLTNDNNKVLSRAEALGRLSRAGFKISQESFAGG
                     YLCIEAVKTGEPIQMNGVNYGPIIALPRVGQNRDPIKVYKLRTMHPYSEYIQDYIYDQ
                     YDLQEGGKFNDDFRITSWGAICRRIWLDEMPMLINLFKGNMKLVGVRPLSLQYFELYD
                     RHLQERRINYKPGLIPPYYADLPSNLAEIQESEIRYLDAFDKNRFLTDFRYFWKSIWN
                     ILFRNARSR"
     gene            complement(15323..16966)
                     /locus_tag="PK288_05430"
     CDS             complement(15323..16966)
                     /locus_tag="PK288_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18396.1"
                     /translation="MTFSPRLAILSFIVICSQAALYAQEVPHPVVNTGIYEFLDELAS
                     LQIIELNSAVKPYSRLFIAKRLGEADRKREVLNTRQQKELDFYLSDFGKEMPPVSRDS
                     GIRQIGGLFRKKGLDPKLRRDLFYYRDSLFSITVNPIIGGEFFQNQAGTATYARNFGE
                     ARAYVGKWGFYASLRDNHEKPLLGKPLFLTKREGGHIKGSTDWSEMQGGITWSWKWGN
                     AGFVKDRQQWGNNYNGANIFGGNNPTFVQLRLNLKPVKWFEFNYFHGWLNSMVVDSSR
                     SYWVNNSYGPDYREVYHKKFIAANMFTITPVKNLNISAGNSIVYTDRHFNPVYIMPLF
                     FYKSVDHSYTSGVDNMNSQTFIDISSRNIRHLHVYSTLFIDELSVARFRRKDEWNFFS
                     WKAGFRLSSFPLNNLSFTAEFTYSYPLTYQHFVPTLTFETQNYNLGHYLRDNSREWYL
                     ALDYKPARALNIGVYFSDAVRGPDYTELGTPRVGNPPLASTEWQSTIAGFRSSYQVIN
                     DLYIWLNISAGNITGDSRWSAAYFYGNKNTFNAGMTYGF"
     gene            complement(17000..17188)
                     /locus_tag="PK288_05435"
     CDS             complement(17000..17188)
                     /locus_tag="PK288_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18397.1"
                     /translation="MQLKRQYIKPEVIKITLDRSVSLMMKSHPFPPDPRGRGGGGPGR
                     KGNDEPIFNSPFKNKPFE"
     gene            complement(17346..>19162)
                     /locus_tag="PK288_05440"
     CDS             complement(17346..>19162)
                     /locus_tag="PK288_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18398.1"
                     /translation="KANLGAEFTHTTSYLNLGGQAMGPGSYGGTGSGADHILTDYFDA
                     ATGVVNNTVQRGTWLGNTTDWNLASNWSGGVPGDTTIARITVAAPNQPVIPTSYSAVS
                     DTVIIGPGASLTINGSADFNSAKINGTLTINPGGRATVDNVASSGTINLESGPSGMFS
                     FMPGSYSGTGTVNTRLFFTGGTAGPGNYRWHYFAVPSQQDKSVVTNVSPLDLMWYYEP
                     AAVTNMRQGWQWHDGYDNTTPISELRIGEGYSFYNDVDSYVTFTGNSLVTSLPQANLS
                     FTTFGWNFLGNSLTCGLDWDAVAFTGSLDPTVHFIKDHREYYYIQGGPGVPAGTTGHI
                     PPLQGFFVQAKAAGASVDFSGAKEHNNIQFYKGSRDNEQKGIYPMIRLTLDNGIHTDE
                     TAIWFDENSTTGKDLKYDAEKVIQKKPAVQIYSSLKGNSYAINGIPFPETSIDIPLTV
                     STAAGGRFTIRQISAENVEELSFYLRDIEQKVRINLKENPRYSFTVPEGTIANRFILT
                     IENTSALKNEESASMKPFNIYASFGLVNIEPLSGQWDGRNGSVKIIDLTGKTLTELRN
                     VEFSRSSLIQVPVGNSKGMFIVEITSSPLRHSGRIIVK"
BASE COUNT         4841 a         4358 c         4571 g         5392 t
ORIGIN      
        1 tattgcggtg atgcttaagc agcagggcaa ggtgttcagg attgaaaagc aggtgcacag
       61 ctatcctcat tgctggagaa ccgataagcc tgtactctat tatcccatgg atgcatggtt
      121 catacggaca acagcattca gggaaaagat gattgaactg aacaacacca taaactggaa
      181 accggtgtcg accggaaccg gccgtttcgg gaaatggctt gagaatcttg ttgactggaa
      241 tctttcgcgt tcgaggtact ggggtacacc gctgccaata tgggtgagcg gggacaggac
      301 cgaggagaaa tgcataggat cggcagctga acttaaggag gagattgaga agtcggtgaa
      361 agccggattt atgaccgcga atccgctgaa ggatttcatc gaaggcgaca attcatctga
      421 aaattacgga aagttcgacc ttcaccgccc gtttgttgat gaaataattc ttgtgagccc
      481 gacaggaagt aaaatgttcc gggagcctga ccttatcgac gtatggttcg actcgggagc
      541 catgccctat gcacaggcgc attatccttt tgagaacaag gaaaatttca gcgacatgtt
      601 ccccgctgat tttattgccg aaggagttga ccagacgcgg ggatggttct tcacccttca
      661 tgccattgca agcatgatct ccggttccgt ggctttcagg aatatcattt caaatggcct
      721 tgtgctcgac aagaacggaa acaagatgtc gaaaagactg ggcaatgcgg ttgatccttt
      781 cactgcaatt gaagagtacg gttccgatcc gctgaggtgg tacatgctta caaatgccca
      841 gccgtgggat aacctgaaat ttgatatcga aggcgttgac gaggtcaggc ggaaattctt
      901 cggaacgctt tacaacacct actctttctt tgcattatac gccaatatcg ataaattcag
      961 gtataccgaa aaagaaatac ctgttgggga gcgccccgaa attgacaggt ggataatctc
     1021 gcttctcaac tcacttgtca gggaggtcgg aaagtgtttc gatgattatg atcttacgcg
     1081 cgcaggccgt gctatccagg attttgtgac ggagaacctg agtaactggt atgtaaggct
     1141 taaccggaaa cgtttctggg gaggggatta tgacaaggac aagcttgcag cctaccagac
     1201 cctttacagg tgtctcgaaa cagtcagcag gcttgcagca ccaatcgcac cattttacac
     1261 ggaccgtctt ttcaccgatc tcaacggaac aacgggacat catcaggagg actcggttca
     1321 tctaacctcc ttcccggtgc ctgatcacga cctgattgat gaagagcttg aggaaaaaat
     1381 ggaaatagcc cagaaaatat cctcaatgat cctcggactg aggagaaaag tcaacattaa
     1441 ggtacgccag ccccttgccc ggataatgat accagtttcc gacgaggaat tccggaaaaa
     1501 gtttgaggct gtaagggacc tcatccttgc ggaggtaaac gttaagaatg ttgaatatat
     1561 cgatgatact tcctcggttc tgatcaagaa ggcaaaacct gatttcagac tgcttggacc
     1621 gcgatacggc cggctcatga agcagatagg taatattgtt tccgggctca cccaggaaga
     1681 aattacttca ttcgagaaca ccggagccct ggtgttcgaa attgatggtc agaaggtgga
     1741 attaaccaca gacgatgttg agataatatc ggaggatatt cccggctggc aggttgcaaa
     1801 tgacggcagg ctgaccgtag cacttgacgt cacggtgacc gatgagctgc ggtatgaagg
     1861 cattgcaagg gagtttgtga acaggattca gaatatcagg aaggaaagcg gatttgatgt
     1921 gactgacaag atcaccgtac tgatcgaaga catggatgtg atcggagcgg cagtgagaag
     1981 gcattcctcg tatatcggat cacagacgct ggcaacaagg gtcgatgtcg tcagggattt
     2041 caccaacggg gatgtccggg atgtggagat caatgatatt caggtaagga tttcggtcag
     2101 cagaaactgc tgatgatgcg ttaatgtctt atttctgcct catttttgca tcgatgctga
     2161 gggttgcgtg aggaacggcc ctcaatcgtt cctttgcaat aagctttcct gtttcgcggc
     2221 agatcccgta ggtcttgttt tcaatcctga caagggcagc ctgcaaatgc tgaatgaatt
     2281 tttgctgccg ttgggcaagc ctgcctgctt cttctttcga taaagtggtg gccccttcct
     2341 caagtacctt gaaggtgggc gatgtgtcca tggtgtcatt actttcttcg tgagtaatac
     2401 tggatttcag tatctcataa tccttcttgg ccttgtccag cttcctgaga ataatttccc
     2461 tgaattcctc aagctcctca tccgaatatc gtgtcttttc agccatggct agttgagttt
     2521 tgtttatggc gcaaatgtag ctttttttgt ttaataaaaa aactgtcagg gtactatgat
     2581 attaaactaa tttcaggctg ttaatattgt catgaggtga ttttatgtgc gaaaaaaaac
     2641 taaacaaaaa agtatctttg cggcggatat tatctattgg agcatgacaa cagaaggcga
     2701 cagcaaaaag gcgttgaaca gactgatcag aaaaacgccg gaaccacgct ggtatgcggt
     2761 ttataccaat cccagggccg aaaaacaggt cagggacaga ctcctggaag cgaacgttga
     2821 agtatttctg cccttgcaga aaacataccg gatgtggagc gaccgtaaga agctgataga
     2881 aaagccgctg ctttcatcct acgtgtttgt aagggtaagt ccggctgaat ttcccagggt
     2941 ttaccagacc cgtgggattg taagatttgt aaccttcgag ggccagccgg cttccatccc
     3001 ccagaaccag attgacaacc tgcggcttct gatcaacagc gacactgaga tagaggttac
     3061 atcaggaaaa ttcgagaggg gcgataatgt tgaagtcgtt cgcggagcgc tggtcggact
     3121 gacaggtgag cttattgaga tcggaagcca gaaacgggtg ataataagaa tcgacaggct
     3181 tgatcagaac ctcattctga aaataccgct tacgtttctc cggaaaacgg gggagaaaaa
     3241 ctgaatcagt tgagataact ccggcataaa ccggtcattt cagcttttat atctgagata
     3301 catggagtaa gtatccttca ggccctcatc caacgggatc ctgtatttgt atccgagacc
     3361 cgtcaaagca gtaatatcaa ttgtctttct cattgttccg tcaggtttcg atgtgtcaaa
     3421 aacaatttcc ccggtgaagc ctgtaagagc tttaattttg taggtaagat cctttattga
     3481 gaactcttcc ccggtgccta tattaaggaa atgcggagga tggaattccg gatcggggtt
     3541 ccctttcctg tgcatcgcct ggatgtcgtc tgcatcgacg ttttccatta tgaagacgca
     3601 tgcctcggcc atgtccttcg aattcatgaa ttccctgagg ggagtaccgg tcccccaaac
     3661 ggaaacggca acttcatgcc ttcccgttcc ggatctttcc gtgagcttta caccgtattt
     3721 gcgcaggatg cccgtaatgc ttccatcatc ggagctgccg cttatccctt caatcgggta
     3781 tctgtcaaga tccctcctga ttgcatccat atcgcccgtc tcaaggcatc gccctaaatg
     3841 gaatttcctg agcaatgccg gaagaacatg agatccttcg agattgtaat tgtcaccgta
     3901 accgtaaaga ttggtcggca tcactgcaat gaagttggtg ttatactgaa tgttaaatga
     3961 ttcacacatc ttaaggccag cgatttttgc aattgcataa ggctcatttg tgtactcgag
     4021 ctgtgatgtc agcaggtatt cttcccttaa aggctggggc gcatttttgg gatagatgca
     4081 tgtcgatccc aggaaaagca gttttttaac gccatgtttc caggcagagc tgatgacatt
     4141 gttctgaatc atcagattga tgtagatgaa atcggccctg agggtgttgt ttgcaactat
     4201 ccccccgact tttgccgctg caagaaatac atactccggt ttctgatccc tgaaaaagga
     4261 atccgttgct ctctgatcgg taagatcaag ctcctctgcg gtcctgagca gcagattgtc
     4321 gtaacctttt tccctcaggg tcagggttag agctgatcct accagccccg tgtgtccggc
     4381 gacatatatt ctggaatttt ttttcatatc tgaaagcttt aatcacacct gtgcatttcc
     4441 tcggaaatgg acaaggcatc ctgggtaatc cgggtcattc cggcacttta gggttagtta
     4501 ttcaaaatag ttaagggtcc tgaatcctcc gtttttaatg tgagcttcct ttttcatcag
     4561 gttaatatcg gaattgatca tatcctgaat aagcccgtca agatcaaatc ttggcgacca
     4621 gccgagtttc tgccttgctt ttgtggaatc cccgatcaga agatccactt ctgtcggcct
     4681 gaagtagttt ttatccacct ttacaacgat ggggttgttt ttgatccttg acctgattgg
     4741 ttcagaaaat tcctcgccca ccatggtagc gaaaagtttt tcgtcaatgg cagtgatttt
     4801 cccgacctca ttttcctttt cacctgaaaa gacaacttcc atcccggcgg cctgaaatgt
     4861 ttttataatg aattcgcgga ttgaagtagt aacccctgta gcgataacat aatcgtcggg
     4921 ctgatcctgc tgaagtatca ggtacatggc ttttatgtaa tccttggcat ggccccagtc
     4981 gcgtcgtgat gaaagattgc ccatataaag acagttctgc ataccaaggg cgattctggc
     5041 catggccctg gaaattttcc tggtaacgaa tgtttcacct cttatgggtg actcatgatt
     5101 gaacaatatg ccgttgctgg cgtgcatgtt gtatgcttcg cggtagttta cggtgatcca
     5161 gtatgcatac agcttggcaa caccgtaggg gctccgtgga tagaaaggtg ttttttcagt
     5221 ctggggaact tcctggacca gcccgtagag ctcacttgtg gaggcctggt agattcgtgt
     5281 cttcctgatc aggcccagaa gccttacagc ttccaggatc ctcagcgttc ctatcccgtc
     5341 ggcattggcg gtgtactctg gtgtgtcgaa gctcaccttg acatggctca tggccgcaag
     5401 attatagatc tcatccgggt ggcattcctg gataagtttt gtaatattca ttgaatcggt
     5461 caggtcgccg taatgaagta tgaaattccg tttttcagcc tggggatcct ggtaaaggtg
     5521 atcgatcctt tcggtattga acaatgagct gcgtcttttt atgccgtgga caatgtaatc
     5581 ctttttaaga agatactctg caagataagc gccatcctgt cctgtaactc ctgtgataag
     5641 tgctgttttt gacatctttt gttattgttt taactatgtg gtttcgattc tgcctatccg
     5701 cattccgctt ctccactact tcgccttctc tcttctccca ctcactctca ctctcacact
     5761 cactccctcc cacactcaca ctcacactca cactcactct cactctcact ctctcccttc
     5821 tctcacatcc cgaagtcttt cattctgatc ttgatcctga aggcgttagg gatctcctgt
     5881 tcgctgatca gtatcaggta tccgcctccg ccggctcccg acagtttcca tgcgctggcc
     5941 gtttccctgt actgatcgat gactctttgt atatcggcat tcatcatttt cgggaacatt
     6001 cgcacctgcg accggaatga ttccaggaat cccttcgtga atctgccgaa atcccggctg
     6061 accaatcctt cccatgcaag tccggccgca tcagcgagcc tttttacatt ttcagcattg
     6121 atatttgtgt cgcttaatac attatatcca ttgggccggg gccatagtgt cagcatatac
     6181 aacctgtcct cgagccattt tattgtattc agatcagaga ttgtctcaaa tttcgacggc
     6241 cagtactcgc ccctgtcata ataaaaccag gtaatcccgg gcatcgttat gccgattgaa
     6301 tcctgcgatc cgctcacttc cgtggttccg ggatcattat catacctgaa gagtaacctc
     6361 gcaagttttt cgggcttttc aaggggaaga tgataattcc aaagctcgat tgcttttttc
     6421 cgggtcgatg ttgccattcc cgaccgctca ttgaattcaa tggtaggctc gagcgatgca
     6481 gtgatagcag cgcccggatg gtattttgaa acatatggct ggtcgatcca tgttccggca
     6541 agatcgatcc tgtacggcat cggttggatc gtgcgcaatg cagtggtgga gcgggccggc
     6601 agattgccgt gaggtatcct tttaagcacc ttgtactcta ttccgtgatc cctgcaaagc
     6661 ttttcctttt caggggaatg gccgtcctcg ttaacgataa aaatgtccgg actgagctct
     6721 ttcaggtctt ctgcaaaatc gagcagtccg cttcccttgt tgacaaaagc tttatgaaca
     6781 cacgacaatg cgctcaggat atagaccctt tctgcttcgg aatttaccgt ttccctcccc
     6841 ttgagctccc tgatggtttt atcggagccc agacctatgt aaagctcgcc atattcagat
     6901 gcttcctgca gaaaggcaat atggcccgag tgaagcatgt cgaaacagcc cgaaactaaa
     6961 actttctttt taatgctcat tatgttattg aaatgctttg gtttgtaaac aaaaataatc
     7021 gaaaaatatt actaaacctc taaatttgca ggcttcttca tgaacctgtg cccagcagcg
     7081 gagatgcagc aatctcccgt tggatgcaga aaactgcaat ttccgggatc ctggtgcagg
     7141 atccgtgtga ctcgaagcag cacgcattct gttcgggatt aataccttct tatcctgaca
     7201 cagactatgg gaggtgccag gaatcatata ccgcaaattc tattatcttt aaaattcctt
     7261 caggttcaat tttaattcca acccggtatg aaagagtatc taaaaacccg caaccacaca
     7321 accttcaaaa gatattgcaa attcctcagg cttcaggatg atcctgttct gattgaaaaa
     7381 tacaggcaga tccatgctcc cgggatggta tggcccgata ttacagggga tatgaaagaa
     7441 gcaggcatcc tggacatgga gatttatatc cacgggaata atgcttttat gataatggaa
     7501 accgttcctg attttgatca tgaaaaggca atgagagctc ttgcctccaa accacgtcag
     7561 gttgaatggg agaattatgt ttcaaggttc cagcatgcgg aaggaagatc cgacacgccg
     7621 gaaaaatggg aaataatgga acgaatattc ctgctggatt aattgtaatc cggatatgcc
     7681 gggacggaag gcgggcttgt cccaaattct accgggatgg gcctggaata tcaaatactt
     7741 ccgggaatta acccagtaat gcagcattga tctgctgtca ttgattatct tttgtttccg
     7801 gcagcaatcc tccggatacc tttcccggat agtatctatt gggttttata gctgccggga
     7861 ggggtcctga agctgattgc caggcggttc catccattga ttgctataat tgccatcgtc
     7921 agggcaatga tttccttttc ggtgaaatgt tccctcacgg cttcatacaa tggatccgga
     7981 acatcatttc cggaaatcag ggtcagtgct tctgtccatt caagtgcagc acgttcgcgg
     8041 ggagtgtaga aaggcgtttc ccgccaggct gacagggtat aaagccgttg ttctgtctct
     8101 cccgccttcc gtgcatcctt ggtatgcatg tcaatgcaat atgcgcaccc gttgatctgg
     8161 gacgcccttg tttttaccag ttcatacagc gtcctttcaa gccccgatcc atgaacgtat
     8221 ctttcaagtt caagcaatcc tttaagggca ccatcgctct ctcttgagta atcgagtcgt
     8281 tgtttcatgt ccatagcttt gattttaatt gttttcaatc catgcctgcc gtgcagtcta
     8341 gacaggccag tgaataagaa tcatattcat cgaaggcagg accgggtctg ccatttagtg
     8401 tgattatccg gtcgagcctg actttgctcc ctgaatccag tgcaaggaat tcttcatgat
     8461 cactattgcc agtaactccc tgtattgtac ctctggcatc gttcagctcc tttgccggac
     8521 cgaaataatg aatgaggact gataagttcc ttttcctgat atcttcaatg atatcataaa
     8581 aatccggatc aaccggtgaa tatttatcat acatgattct gatctttttg tgaaattcaa
     8641 aaaacactgc cggcttgccg aaacacatat tcctgacgcc tgtggttagg gcgggcaaat
     8701 ttttccaatc tttaaatccc tgctttttat ccggactatc agggaatttg tgtcagtact
     8761 ccgggagtaa agatagaagg attttattaa aagacaaaaa agtcttttaa taaaatttga
     8821 ttttttaaga atcagccgga aaggaatttc cgcgccaggg gatcagcagc gcaggtgagg
     8881 gtgtataaat cagcatggtc ctggcctgtt cagatgcttt cattttccgg aggggcggga
     8941 atgggaatcc gggctgcgtg cgaacaactg ttcaatgagc cattgcggcc tgtctgtggt
     9001 aattcccatg actcccagcg atatcagtcg atgtgcctca tccctgtcat taaccgtcca
     9061 ggcaaatacc tccaggttca tttcagaggc tctgtcgaca atttccctgg ttattaacct
     9121 gtaattcaga ctgaatccct gtagtccggc ttctttcact ttatgaaaat tttcctccag
     9181 cagggaagga ttatcgcaaa gccagtagca cggatattgg ggaaaggctt ctctggtcgc
     9241 ggtgattgtc ggaagatcga aacaaatgaa tgtgaatttc cttttcgccg catatccggc
     9301 aatggtttcc ttgaggagag gcagtacctc tggtccgcat tttatttcaa tcacaatttc
     9361 ctttcctgca ggaacggttt ttataagttc atggagaaaa ggaagtttct gccccttgta
     9421 tttcggatcc ttccatgaac cggcatccag ttttctgagg acgtcggagt ttgtttcact
     9481 tattttatga tcgtgtccgg tggtcctttt tgtattgccg tcgtgcgaca cgattatctt
     9541 attgtctttc gacagccaga tatcacattc aacggcatca gccccgtgct tccatgcaag
     9601 ttttgctgag gcgatcgtgt tctccggagc cagatatgat gctccgcggt gagctattat
     9661 gcttacctgt gcgcggataa aagttgaagg gatcagcatg cagatggtta ataaaatcat
     9721 ttttttcatg tggaaataaa ttaataaact actgaagatt aagtgattca catggaatct
     9781 acatgaattt tattttcatg tttgtttgtg attttgtatc atgtgggttt cataatattg
     9841 ttattttttt aaagcctgcc ggttacaggc cggtcagggg gagtacccgc acagaattat
     9901 tctgaagcct ggcaatgctg tgaacagaag gggattgtaa taatcatacc gttccctgtt
     9961 tcccccgttt acggtgattt ttcgatttct acctgcaatt tacagaaaaa atggtttgaa
    10021 cggtcgtccg gacagggagt gacaagcagt cttttattaa ctgcagggga ctgtcccgac
    10081 aaattagtgc aatatatcct ttcaccgacc ctcattcttt atgaaatatg aatcaattga
    10141 cgtatatttg ataagagtga gagatcggtt taaaaaggcc ggtgtgatgg atcagttaca
    10201 tcaccgatgg cagaaaagga tttaattgta acgatgaggc agtttttccc atatatcggt
    10261 atttgtataa tgctcgttct gtttacgggg gtgatgtccc gggccgggga ggacagcgga
    10321 agggggccgg tggtggtgat ctctctggat ggttcaatca atcctgcaac ccaggaatac
    10381 attaattcgg gtatcaggaa tgcacgggaa aagaacgctg agtgcctggt gatcaggctg
    10441 aacacacccg ggggattgtt aaaatcgacc cgggtgattg taaccgatat tctcgactcc
    10501 gctgtcccgg tggtggtttt tgtttatccc gggggatcgc aggcagcatc ggcgggtgtt
    10561 ttcattaccc ttgccgcgca tattgctgcc atggcgcccg gcaccaatat tggtgctgca
    10621 catcctgtga ccatgcaggg ccagcaggat tccatcatga atgaaaaagc caccaatgat
    10681 gccgcggcct ttatcaggac aataagcgag aagcgtgaac gaaatatcag atgggctgaa
    10741 gatgcggtca ggaaaagctt atccattacc gaaagcgagg cattgaagga aggagttatc
    10801 gatcttgtcg ccgtatcagt ggaggatctg cttgaaaaga ttgacaaaag ggaagtggtc
    10861 ctttcttccg gcaagaaggt tctggatacc aaaggcgctg aggtaattaa tatggaaatg
    10921 aatttcaagc agaagatcct cagcctgctc agcgatccga atatcgccta tatattgctg
    10981 atgataggaa tatacgggat catgtttgag ctgtacaacc ccggcgcaat atttccggga
    11041 gtaattggcg ggatcagcat aatacttgca ttttactcct tgcataccct gccggttaac
    11101 tatgctggcc tggcactgat cattttcgcc gtgatcctgt tccttgcgga gattaaggta
    11161 gtcagtcatg gcctgctggc aatcggcggg gtcatatcgt taatacttgg atcaataatg
    11221 cttataaata ctgaaacaac catggaagtg atcaaaatct cctggcaggt aattctgata
    11281 atcgtcatcc tgactgccgc tttcttccta tttgcaataa gtttcggaat taaggcacag
    11341 ggacgcaagc caacaaccgg gattgaagga atgatcggtg agaccggtga ggcaatcact
    11401 aatctggatc cggagggaca gatcagtgtg cggggtgagg tctggaatgc tgaaagcctt
    11461 gaaggccccg tcagcaaagg aaccagggta aaaataaacg gagtttcaaa tctgaagtta
    11521 ttgatccgga aaacaacata acaacactat aagggaaaat cattatggag cagacatctt
    11581 acacactatt aattacggga ataatattcc tgttgatcat ccttgcaagc gctgtgcgga
    11641 tactgcgcga atatcagcgt gctgtgatct tccgactggg aaggctgatc aaggtcaagg
    11701 gtccgggcct gatactgctt ataccggtta ttgacaggat ggttcgggtg agcctgagaa
    11761 ctgtcgtcct cgatgttccg ccgcaggatg ttatcaccaa ggacaacgtc tcaataaaag
    11821 taaatgcggt tgtctatttc agggtgatac agcctgaaaa agcaatcgtt gaagtggaga
    11881 attacctgtt tgccacttcg cagctttcac aaaccacgct gcggagcata ctggggcagt
    11941 ctgagctgga cgagctgctg tcgcaccgcg acaagatcaa tcatgagctg gcaaaaataa
    12001 ttgacagtca gactgaacca tgggggatca aagtctcgat ggttgaggtg aagcatatcg
    12061 acctgccgca ggagatgcag cgtgcaatgg caaagcaggc tgaggctgaa agggaacgaa
    12121 gggcaaaggt cattcacgcc gaaggcgagt ttcaggcaag ccagaagctt tccgacgcag
    12181 ccagtataat aagcaggaat ccttcagcat tgcagctccg gttcctgcaa accctcaccg
    12241 aggtggcttc ggaaaaaagt tcaacaatag tcttccccgt tcccctcgac ctgatttcgg
    12301 cctttatgcc gtctgagaag aaataaggga ttacggaaaa atagtatcgg tcagttgatt
    12361 ttttatggtg ctgcagaaca aggacagctg gacgattacc attaagtgag cttcagaagc
    12421 gttgtttctc tcacaattgt ctttactccc gctctcacat cacacctccc ctttaatttg
    12481 aatcggattg acctgtgtat tccataaacc attatcttta aaaaacaata atattaccat
    12541 ggccaaatac ctgatcaccg ggactgccgg cttcattgga taccatattg caaagagact
    12601 catcggacac ggcaatgaaa ttgtcggaat tgacaatgta aatgattatt atgatgtgaa
    12661 ccttaagttt gcccgcctgg aagatactgg aatcatgagg gatgaggtga gcgggaaaga
    12721 attaatccgg agcaggaaat ccggggaata cagattcaaa aagatgaatc ttgaagataa
    12781 cgacagttta ttagcgcttt tcagagacga aaaattcgac tatgtcattc atcttgctgc
    12841 ccaggcagga gtaaggtaca gcattgaaaa tccgggagtc tacatcagca gcaatatcac
    12901 cggtaccttt aatattcttg aggcatgccg tcattatccc gtaaagtttc tgatgtacgc
    12961 gagcagttcg agtgtttacg gtaatagtga aaaagtacca ttcagcgaag atgacaatgt
    13021 tgacagtccg gtgagcttgt atgcagcaac caagaagagc aacgagctta tggcagcttc
    13081 ctacaatcat ctctacggca ttgaatcgac gggtttgcgt ttttttactg tttacggacc
    13141 gtggggaagg cccgatatgg ctatttttaa gttcacccgt gctatcctga ataatgagcc
    13201 gcttcaggta tttgccggcg gtcgcctgct tcgtgatttt acttatattg atgacattgt
    13261 taacggaatg acggcaatcc taaagcagca aactgtcagt gcggctccgt cagtaatgaa
    13321 cattggtaat aacagcccgg ttacagtcag ttcgctgatc tcagagcttg aggaatgtct
    13381 cgggcgaaag gccataatta actatctgcc gatgcagcag ggtgatgtaa aggccactta
    13441 tgcaagcatt agcaggatcg gtgaatactg cggatttgca ccccgtactg atcttaaagc
    13501 cggcttgagt aaattctgca gatggtacct tgattattac ggcatccgtt aatggatgct
    13561 gcctgccttt cttctccgac aggcagcctg ttgtacctga tttccaacat ttcattttcc
    13621 cctgcatata cacactcact ctcgcactcc cactcactct cactctcact cactctcaca
    13681 ctcacactca cactcacctg ctccttgcat tcctgaacaa aatattccat attgatttcc
    13741 agaaatacct gaaatcggtc agaaaccggt ttttatcgaa ggcatcaagg tatctgatct
    13801 ccgattcctg gatttccgcc aggtttgatg gcaggtcagc atagtaggga gggatcagtc
    13861 cgggcttgta attgatcctt cgttcctgga gatgcctgtc ataaagttca aaatattgca
    13921 ggctgagggg cctgactcca accagcttca tgttgccctt gaaaagattg ataagcatcg
    13981 gcatttcgtc gagccatatt ctcctgcaga tagcccccca ggaggttatc ctgaaatcat
    14041 cattgaattt gccgccttcc tggagatcgt actgatcgta aatataatcc tgtatgtatt
    14101 ccgaatacgg gtgcattgtg cggagtttat atacttttat gggatccctg ttctgcccga
    14161 ccctgggcag tgcaataatg ggcccgtaat taacaccgtt catctgtata ggttcaccgg
    14221 tcttaactgc ctcaatgcag agatagccgc cggcaaagga ttcctgggat atcttgaatc
    14281 cggcgcgcga tagccgcccg agggcctctg cgcgtgaaag gaccttgttg ttgtcgttgg
    14341 taagaaggtg gtaaagcttc cttgtgaatt tgattttggg caggatccgt tttaccacaa
    14401 agtcggcact gtaataaaaa taattcagca caggcgggta tttcttcagc aatcgttttt
    14461 tcctctgatc cttggtctca acgcatccaa gaaaatatcc tttaaccgga agcttgaaat
    14521 taacttcgtc gaggaagtaa ttgagttttt tgatatcatt gaacctgtgc agattgatga
    14581 tgtagtcgta ttgttcctcc ggcaggtttg tgatgttgaa tattgttgtg gttgagacca
    14641 cggcagtttt tttgttgagc ctggctcccg caatattcat tattgcccgg gccatttcag
    14701 ctcctgcttc ccgttcaatt gccctgataa ccctggggtt aatgtcattt tcattaatgt
    14761 gaatgttccc attcccgttg ccgttagttt tttcgacaag ttcctcttcg ctctttttcc
    14821 tggtggcctt atattcatcg taattctggt aatcctgtat tacggctttc ttgtattcta
    14881 tataaatggt gccgatcaga agttcaagga atgttgcaat gcatgcagtt ccaagcacca
    14941 cggtgcggga gaaggaatat tccctgaagg catacatcaa tagtgcaatg attgccaggg
    15001 cgataaaatt gcttgttaac accctggaaa aaagtgtcgt gaagtttacg atgcgccccc
    15061 tgtgcatttt gccgttcagc agcgaaataa ttatccagat aagtgaaagg ccggcaagaa
    15121 agatataatg tgtgggaaga tatcctgtta aaccggaagg cttgaaagag gctgctatca
    15181 gaaatgatat tgcaagtatt acaatatcag caattaaggt aaaagtcctg aactttacgg
    15241 ctttcattgt tgtctcacaa tcctgatgtc caaattaagg caataaaatt gatacctgaa
    15301 accggtatgg tgaaaagttg atttaaaaac cataagtcat cccggcatta aaagtgtttt
    15361 tatttccgta aaaatacgca gcgctccatc tgctgtcgcc tgtaatattg cctgcagaga
    15421 tattcagcca gatgtacaga tcgttgatga cctggtatga tgatctgaat ccggctattg
    15481 tactctgcca ttctgttgat gcaagcgggg ggttacccac acggggtgtg ccgagttcag
    15541 tgtagtcggg gccgcggact gcatcagaaa aatatactcc gatattaagg gctcttgcag
    15601 gtttgtaatc cagcgcaaga taccattccc ttgagttatc cctcagataa tgacccagat
    15661 tgtagttctg tgtttcgaaa gtaagggtgg ggacaaaatg ctgataggtg agagggtagg
    15721 aatatgtgaa ttcagcagtg aaagacagat tgttaagcgg gaaactgctt agcctgaatc
    15781 cggctttcca gctgaagaag ttccattcgt cttttcgtct gaacctggca actgacagtt
    15841 cgtcaatgaa aagagttgaa taaacatgca gatgcctgat attccttgaa cttatatcga
    15901 tgaaggtttg cgaattcatg ttgtcgaccc ccgatgtgta ggaatgatcg actgatttgt
    15961 agaaaaaaag cggcatgatg taaacggggt tgaagtgcct gtcagtgtaa acaatgctgt
    16021 tgccggcaga gatgttcaga tttttgacgg gggtgatggt gaacatgttt gcggcgatga
    16081 attttttgtg atacacctcc ctgtaatccg gtccataact gttattcacc caatacgatc
    16141 ttgaactgtc gacaaccatt gaattcagcc atccgtgaaa ataattgaat tcaaaccatt
    16201 ttaccggttt aaggttcagc ctcagctgaa cgaaggtggg attgttgcca ccgaatatat
    16261 ttgcgccgtt gtaattattg ccccattgct ggcggtcttt tacaaatcct gcattacccc
    16321 atttccacga ccaggttatg cctccctgca tttcgctcca gtcagtagat cctttgatat
    16381 gacctccttc cctctttgta aggaagagag gttttccgag aagcggcttt tcatgattgt
    16441 cgcgcagtga ggcataaaat ccccacttac cgacataggc ccttgcctca ccgaaatttc
    16501 gggcataggt tgctgttcct gcctggttct gaaaaaattc tccgccgata atcggattta
    16561 cggtgattga gaataaagaa tccctgtagt aaaacaggtc tcttctcagt ttcggatcca
    16621 gccctttttt ccggaataac cccccgatct gccttatccc ggaatccctg ctgacaggtg
    16681 gcatttcctt accaaagtct gaaagataga aatccagttc cttctgttgt ctagtattca
    16741 gcacctcccg tttccggtca gcctcaccga gccgttttgc aatgaacagt cttgaatatg
    16801 gtttgaccgc tgaattcagt tctatgatct gaagcgaagc cagttcatca agaaattcat
    16861 atataccggt gttgactaca ggatgaggga cttcctgggc gtaaagtgct gcctgactgc
    16921 aaatcacaat aaacgacagt atggcaagtc gtggtgaaaa ggtcatggga tgcatataat
    16981 acagcaaacc ttaaacgggt cactcgaacg gcttattctt gaaaggactg ttgaatatgg
    17041 gttcatcgtt acccttcctg ccaggtccgc cgcctccccg tcctcgcgga tcaggcggaa
    17101 aaggatgcga cttcatcatc agggataccg acctgtccag cgttatttta attacctcgg
    17161 gtttgatata ttgtcgtttc aattgcattc cctgtttaat gaaatgttgt tgcagcaaca
    17221 attatccgtt attaatgagt acaaaagtaa tccaattgcc agaattatca aatttttatc
    17281 agttcacttt tctgaatatc cgttccgcct gattttccgt gtcttttcag ttcagtccct
    17341 gttaattatt taacaatgat ccttcctgaa tgcctcagtg gagaagaagt tatttctaca
    17401 atgaacattc ctttactgtt tccgacgggc acttgtataa gtgacgacct gctgaattca
    17461 acattccgca attcggttaa ggttttcccg gtgaggtcaa ttattttaac ggaaccgttc
    17521 cggccgtccc attgcccgct caatggttcg atattgacaa ggccgaatga agcatagata
    17581 ttgaacggtt tcattgaagc tgattcctca tttttcagcg ctgaggtatt ttcaattgtc
    17641 agtataaacc tgttggctat ggtaccttcg ggaacagtga aggaatacct gggattttcc
    17701 ttaaggttta tcctgacttt ttgttcaata tccctcaggt aaaaggacag ttcctcaacg
    17761 ttttcggcac ttatttgcct gatggtgaat ctgcctcccg cagcggtgct tacggtcagc
    17821 ggtatgtcaa ttgacgtttc ggggaatggt attccgttta tggcgtatga attacccttc
    17881 aggctggaat agatctgaac tgccggtttc ttctgtatca ctttttcagc atcatatttc
    17941 aggtccttgc cagttgttga gttttcatcg aaccatattg cagtttcgtc tgtatggatg
    18001 ccgttatcga gtgtgagcct gatcatggga tagattcctt tctgttcatt atcccttgat
    18061 cccttgtaga actggatgtt gttgtgttct ttggcgcctg agaaatccac tgaggctccc
    18121 gcggctttgg cctgcacaaa aaagccctgc agcggcggta tgtgtccggt ggttccggca
    18181 ggtacgcccg gcccgccctg gatataatag tattctcggt ggtccttaat gaaatggaca
    18241 gtgggatcca ggctgccggt gaaggccact gcatcccagt ccagaccaca tgtcagcgag
    18301 tttccgagaa aattccagcc gaatgttgta aagctcaggt ttgcctgggg cagtgaggtg
    18361 acgagggaat tccccgtgaa cgtcacatat gaatcgacat cattgtagaa gctgtatccc
    18421 tcgccgatcc gcaattcgga tatcggtgtc gtgttatcgt atccgtcgtg ccattgccat
    18481 ccctgtctca tatttgtaac ggcagccggt tcgtaatacc acatcaggtc cagcgggctg
    18541 acattggtaa caaccgattt atcttgttgt gaaggcacgg caaagtaatg ccacctgtag
    18601 tttccgggtc ctgccgttcc cccggtgaag aaaagcctgg tattcactgt tcccgtaccg
    18661 gaataactgc ccggcatgaa cgagaacatg cccgaggggc ctgattcaag gttgatggtc
    18721 ccgctgctgg ctacgttatc aacagttgcc ctgcctccgg ggtttatcgt gagggtgccg
    18781 ttaatcttcg cggagttgaa atctgcgctg ccgtttatgg ttaatgaagc gcccgggcca
    18841 attattacag tatcacttac ggcactgtaa gatgtcggga tcacaggctg attgggtgca
    18901 gcaactgtga tccttgcaat cgttgtgtct cccgggacgc ccccggacca gttggaagca
    18961 aggttccagt cggtggtgtt gccaagccat gtacctcgct gaacagtatt gttcaccacc
    19021 cccgtggcag cgtcgaaata atccgttaga atatggtcgg ctccggagcc agtccctccg
    19081 tatgaccccg ggcccatcgc ctggccgcca aggttgagat agctggttgt gtgtgtgaac
    19141 tccgctccca ggtttgcttt cg
//
LOCUS       DAOOYG010000042        18918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2805_length_18918_cov_6.349838, whole genome shotgun sequence.
ACCESSION   DAOOYG010000042 DAOOYG010000000
VERSION     DAOOYG010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_18918_cov_6.349838"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1593)
                     /locus_tag="PK288_05445"
     CDS             complement(<1..1593)
                     /locus_tag="PK288_05445"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM18399.1"
                     /translation="MRKIAILLAFLLFAGLQGAFAQKTITGKVTSAEDGLGMAGVPVV
                     VKGTTIGTATDIDGAFTLSVPANAAALVVSFIGMKTVEVPIGTQTHFDIVLEQNILAL
                     EDVVVTAFGISRQAKALTYAAQNVAPKALAEARDLNPVSGLSGRVAGLSVTTAGTGVG
                     ADAKVLMRGNRSIAGSSQPLYVIDGVPMGGISNLAPDDIVSISVLKGANAAALYGSRA
                     NNGVIIVTTKSGQGGAEGVMVDLGFTYQGTSAILLDKMQNEYGQGGNGVYSPLAIVSW
                     GPKMDGSQVAHWTPDPNHPLYGKTYPYLPQPNNVKDYFQTGNSIATNLQVTINTARTN
                     TAFSYTNTNASGIVASNNLHSHNLNLRFGAKLGEKFTLDSKVTVIKQSFENIFATGEG
                     FDNPMRYLYVLPRNIRTEDLQHFEYVTPAGQVRQHYWRWNDNGTGNAYWTRNRVLKPQ
                     DRWRPIGMVSLKYQITKDLSIMGRSALDASFTQREEKWYNDTYTVAINGGYGKYNSSS
                     YGWNSDFLINYHKALGDFVFDVN"
     gene            complement(1886..2662)
                     /locus_tag="PK288_05450"
     CDS             complement(1886..2662)
                     /locus_tag="PK288_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18400.1"
                     /translation="MYRYNNISAALILVILFLFSCRNDSPQDLSLRPEEYQKLGLPDH
                     IRLWNLQDYINANITLSTLKTNFPNSLPRKSSKKSGAVFARLVSGDNLSFMYDKSMPL
                     HGRAQIIQHFTRFYSELESIYSVETDVRNYYSDELADINLFGLLVHDRMLELAWQIMD
                     SDDEEDRDLQYGLKTVKRNYLNLVDHLLDEQVKSEVYSDADLDRLSDRLEQSLAVNVS
                     WFLPSERDSVAESIEKAMPECPTAHIRGNFNKMLKYLKNS"
     gene            complement(2767..6087)
                     /locus_tag="PK288_05455"
     CDS             complement(2767..6087)
                     /locus_tag="PK288_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPM18401.1"
                     /translation="MRSLHTGKYILIAAAAVAVTILQFSCGRKNDRLFTSMPPERTGI
                     KFRNWIEEDDVFNVLDYGYLYNGAGVAIGDINNDGLPDIFFSGNFAHARLYLNMGNFR
                     FRDITDVSGVNGGNKWNTGATMADINGDGFLDIYVCCSTDGRYDRRKNLLYLNNGDLT
                     FTECAARYGINDASYSTHSAFFDYDKDGDLDLFVMNHSLDKYAMTNPARKKERNPIYE
                     HRLYKNTGEKFVDVSAEAGMIGNIINFGLGLAIADFNNDGWPDIYICNDYSEQDYFYI
                     NNRDGTFSEKLEDYFDHISFSSMGCDAADFNNDGFIDLFTLDMEPEDNYGNKLVAGPD
                     NYEKFQNLKKSGFFNQSTRNMLQLNMHGDYFTEIGQYAGVFATNWSWAPLFCDFDNDG
                     YKDLYITNGYGRNSTYMDAVVLAVSQMVRQQRGQTHLSKMNVIDQIPPTVLNNYIYRN
                     NGDYTFTDVTGIWGDEKATLSGGAAYADLDNDGDIDLVISTINGFPLVYRNNSEKISR
                     NHYLKIRLKGSGMNTGGIGSRIDVTCGKNTYTQEFMPSRGFMSSVNHEIIFGLGGAAI
                     ADTLRIRWPDMREQVLTAVKADQCIVLNNDDAMIPAPRISSQPEPVFTMVKNNRILDF
                     KHQENEYNDFADQALLPHFLSTQGPRIAKGDVNNDGLEDLYFGGSMGCPGKLYIQSGD
                     GSFRYVPQACFEKDKGCEDIGAVFLDADGDNDLDLYVVSGGNEFHADAAELQDRLYLN
                     DGKGSFSAAKGRLPVMITSGSCVKSCDIDGDGDIDLFVGGRLIPSRYPFAPRSYILEN
                     DGKGFFKDVTDAYNSELMYPGMVTDAVWTDFNRDGKPDLIVVGEWMKIRFFRNEGKRL
                     EEITSLCGPENTEGWWNTIVAADLDNDGDDDYIAGNLGLNSRIKVSPAEPCTIYARDF
                     DNNGTIDAIMSYFIQGKSYPFYSKDDLQKQMPFIKQKYPTYESYANLTITDIFSGDAL
                     SGAEIFEAKTFSSCYIENKGNDRFEIRPLPREAQYFPVYGVCTGDFDSDGNTDIVLAG
                     NFFGSRIKFSDYDAGKGLLLRGDGKGGFTALNDQRSGLFIRGEARDVTEIRTAHGNKL
                     IVFALNNDSLKLYKRESK"
     gene            6364..6720
                     /gene="ssb"
                     /locus_tag="PK288_05460"
     CDS             6364..6720
                     /gene="ssb"
                     /locus_tag="PK288_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPM18402.1"
                     /translation="MNSLKNRVNLIGNLGQDPETKNLENGKKVTRFTLATSDEYRNAE
                     GQKVKETTWHNIVAWNGLADIAGRYLKKGAQIAVEGRIVYRNYEDKNGATRYVTEVVI
                     NEMLMLRSQKAQGREE"
     gene            complement(6876..7442)
                     /locus_tag="PK288_05465"
     CDS             complement(6876..7442)
                     /locus_tag="PK288_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPM18403.1"
                     /translation="MKNDIILSGVGGQGILSIAAIIGLAVIENNLHIKQAEVHGMSQR
                     GGDVQSHVRISDKPVHSDLIPWGQADIIISAEPMESLRYLPWLSSDGWLITSSEPFVN
                     IPDYPPLEDILHEIRKLPKHRIIDADAIAREAGSVRAANMVILGAASPFIKMPFESLE
                     NAVRILFRRKGEEIININLEALRMGRKF"
     gene            complement(7439..9067)
                     /locus_tag="PK288_05470"
     CDS             complement(7439..9067)
                     /locus_tag="PK288_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPM18404.1"
                     /translation="MQKQLLLGDEAIAQAAIDAGLSGMYAYPGTPSTEIMEFIQASDE
                     AAERMIHREWSSNEKTAMEAALGMSYAGKRAMVAMKHVGLNVAADPFINSSVTGVNGG
                     LIVVAADDPSMHSSQNEQDSRFYGKFSLVPVYEPSNQQEAYDMVFDAFELSEKYRLPV
                     LFRITTRLAHSRSAVTRRQSEPQKKLSLPSDPRQFVLLPAIARKKYRALLEIYESLKY
                     DNRLSRHNIFRDDRNRKTGVLACGLAYNYFTENFPDSEPDFPFLRICAYPFPKELITD
                     LTSRCEEILVLEEGYPLVEEALRGILDNNLKIMGRLDGTLPRDGELNPTLVAKALGMT
                     IRKGFEIPGVVRPRPPSFCKGCGHADTFNAVIEALRPFDRDGVFSDIGCYTLAALPPY
                     SIINSCVDMGASVTMAIGAADAGLFPSVAVIGDSTFTHSGMTGLLDAVVKKSPVTIII
                     SDNSTTGMTGGQKSQGTGRLRKICEGLGVAPEHVREIVPLRKNHAENVRIMQEEFSYR
                     NVSVIISSRECIQTASRRRKAESLEVNDPGEAEQ"
     gene            9224..9913
                     /locus_tag="PK288_05475"
     CDS             9224..9913
                     /locus_tag="PK288_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM18405.1"
                     /translation="MAKRLANTTFLFFIFFLATRGQAVQKERFFQLSGIITDENNRAL
                     SNVGVISLRLKRGVLSENTGIYSITSTPGDTILFKALGFKKQMIVLPMSFEGKRYYAD
                     IVLSMDTIPIENVVVLPWKSYNEFIRDITSPNPVDPEIENMNKNIESVKSALANQADV
                     RISPEAGYRYAMQQNFNMTATRNQYPSNNLLNPFAWAKFINGLKSGLLKNDKAEKPSR
                     TKVKTKKKKKQ"
     gene            9910..10668
                     /locus_tag="PK288_05480"
     CDS             9910..10668
                     /locus_tag="PK288_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPM18406.1"
                     /translation="MTNYHDKIAGLLGTVILHLIAGIIFVSFKLHTLKVRQETIQRLE
                     LEMVDEHDLKPREAPKEIPMTSVENIFRDDAEMLNIARNLASRSEIKIDRNDYIDKVK
                     EELIQSGRLGTDNYIDNPGSGYQEAEEDISNGDDEEALRAKEEEKVMESQKMAASYSG
                     PTRIYYDLAGRNHIYLPIPIYKCQGSGKVVLIIDVDQKGNVTSASVVAGESTTSDECL
                     TETAVTSARRSRFNADLNAPKSQRGTLTFIFVAQ"
     gene            complement(10727..11452)
                     /locus_tag="PK288_05485"
     CDS             complement(10727..11452)
                     /locus_tag="PK288_05485"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPM18407.1"
                     /translation="MCFTVNVNLVKDELESRFGVLFPDADRFRPSYYYHAFTLPELPV
                     VCTGSPGRIELMKWGLIPFWTKSAADAEEIRYKTFNARAESLFSKPSFSNPAKSKRCL
                     VPVRGFFEWQHTPGGKIPWYIYHSDNDIFSLAGIYDQWTDSLTGNVTESFSVITTSAN
                     EMMAVIHNSRKRMPAILDKKDEELWLNAGLTEAELISLLKPYPSEGLKAHTVSRLINS
                     KSADRNTSEIIRPFDYPDRTLPF"
     gene            complement(11452..12756)
                     /locus_tag="PK288_05490"
     CDS             complement(11452..12756)
                     /locus_tag="PK288_05490"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPM18408.1"
                     /translation="MKKIILIIFFAVFMAHDPGAVENPGDVRDKLTVGNIRLTNSDSF
                     ENVLGKNDWIIRSFMDKWKIQGASLAVARDGKLAYARGFGFADTASASEVQPYSKFRI
                     ASISKLVTAVAIMKLHEEGRLDLSDRVFGPDGILNDSCYCYPKDKRVFDITVAHLLSH
                     EGGWSQKYGDQMFMPRVVSKQMNVPLPVDLNTIIRFALGKRLHFTPGTGRSYSNLGYA
                     ILGLVVEKVSGMTYDSYCREEIFEPLGIYDMLPARNLESQKARFEVTYYEPFGTPPGP
                     SIYATDELVPVRYGGNDVETLGGAGYWLATAPDLMRLLLAVDGFVAPEDILSNSSIRF
                     MTDMANRFAPVGWKATLIDGNWWRTGSFSGSAGMMKRQPDGISWVILLNSSAWNGPEI
                     HSHINRMMAKFIARTDEWPENDLFEYSLPVPVKVDVPAPKYE"
     gene            12921..14357
                     /locus_tag="PK288_05495"
     CDS             12921..14357
                     /locus_tag="PK288_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM18409.1"
                     /translation="MLSTKLILRHFRTILLSLLCSGPLHAQRSSIDTTDYLPYYYDQA
                     AEFNLIIAASKGYSSEIDRLISKGANPEAETIEGATPLIYAVANNHTDAVKSLLAHGV
                     DVNKTTENNLTPLLISVKNQNIEISEALIRAGAEINKTDRYGAAPLHYAVIYGGFYIT
                     DLLLYYEADCNLKAGDGTTPLMAAIWAGYADIADLLIQNGANMEARDKDGFTPFLIAA
                     QNGDTLLMNLLLKYGVDLYEKNAFNFDALGIAIESNQKPAVEMLLSRGNEWNSGDHSG
                     INPYQIAAASGRKEFRDMLEKKNVPGRIQPSISEMAVLLSTRVTGGGLFTGVSLLFRD
                     PMTEAGFMAGFDIKPWYSRVMIQDNETLFYQYRDRSALAYAGIFKDFRLTGDYRKTRL
                     MLTASISAAYTFGNKLRGTNIEPESKFRLNPGAGIKLQKKRLMLTAGLEYLNSPYYRV
                     GPVWLRTGLAWNIQFRKTRSPGKTIKWY"
     gene            14348..14785
                     /locus_tag="PK288_05500"
     CDS             14348..14785
                     /locus_tag="PK288_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18410.1"
                     /translation="MVLIRMKEAIRIFAIILMVLCFSCEGSKWLVNCEDCYSEEPVET
                     VVRIKLRDIQAPVTIKIYDGDLEDNIILTSFEAYGSEYTVNTGINKKLTFTATYNISG
                     RVYIAVDSTVPRVKYEKDLCDNPCYYVYDNIVDLRLRRTVPGS"
     gene            complement(14803..15711)
                     /locus_tag="PK288_05505"
     CDS             complement(14803..15711)
                     /locus_tag="PK288_05505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPM18411.1"
                     /translation="MFPADLSGLSRLSVPGKIEKRALGRTGEMLSVLGFGGVVVMDES
                     HEDASLLVKMAISSGINYFDVAPTYGDAEIKLGPALEPYRKDVFLACKTTERTREGAR
                     KELEQSLKNLRTDHFDLYQHHAVTLMEDVDRLLGPGGAMETFIEARKEGKIRFIGFTA
                     HSVQAAMALMDRFDFDTILFPFNYATWYAGNFGPQVMEKAHQKNLGILALKAMARGPW
                     KDGADRSKYPKCWYEPLSTPDEIRMGLRFALSHPVTAVVPSGQTELYKLVLEMRNELG
                     PLSNEEVAEIRKKGLAGVPIFRNEDA"
     gene            15943..16542
                     /locus_tag="PK288_05510"
     CDS             15943..16542
                     /locus_tag="PK288_05510"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPM18412.1"
                     /translation="METSFFNSATFNYIIIPVMIFLARICDVSIGTMRIIFVSKGKKN
                     IAPVLGFFEVLIWITAISKIMQNLDNYINYVAYAAGFATGNFIGMIIEEKLAMGIQMI
                     RVFAHERGRDLVQILNSNGFGATSVEAHGAIEKVHLVYSIVHRHELANVLELINEFNP
                     KAFFTIEDVKSVNEGIFTPRKPATILPFSNILREWRKGK"
     gene            16582..17511
                     /locus_tag="PK288_05515"
     CDS             16582..17511
                     /locus_tag="PK288_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18413.1"
                     /translation="MNDFSRHPLYRIHTLNSAMSSMWAFYRKNFTVLFIMSLAVSLMT
                     QFVSTRIDFAGMQTITDPQEMLSEMRGYAAPLLLIAILNLLFTTVLQYYVIYNPVDES
                     NNIVRSLVSSLRYFVPYIIILILLAFFGSMLIVLGLAALIVGAFLAALYVFTVYLFIL
                     PVMMSEGPSIANTISRTIRLAHRNFWTNAGWTAVFLVILIVVTVIFSGLILLPFSGSF
                     LKVFTNPEAAGAVAELASNPLYFILTAVVNALVFPLMPVFACILYFNGRAGEDQISDA
                     GANRDEDNGRNVRIEDLYARPSPEEDTAAGEKE"
     gene            17594..18586
                     /locus_tag="PK288_05520"
     CDS             17594..18586
                     /locus_tag="PK288_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18414.1"
                     /translation="MKNAAVVGFGFMGMTHARNIMKLRDLHLSAIVDPNPALFSGTAP
                     VKSGNINTGDSPAGEFESVSKYADLDKCLDSEDIDAVIICVHTGLHYEMAMKALGRGK
                     HVFVEKPFCLDISQAQEIIDLSRQKDRILMVGHVVRFMSPYRLLKEWSGNETFGKPEF
                     FHFWRFCGVPAWGQWNEEQTVKASGGALFDLVIHDIDFASYVLGAPDDIRSVSLAGRQ
                     SSHDYISAIWNYPSSGINVRIEGGNIFHGSFPFTAGYSARFERASVHYSTSNGNEIII
                     SDETEVKKIPTGDPGAGYYEEMSYFSDCIATNRQPSLCMPESSLGSVALCYRHI"
BASE COUNT         5049 a         4459 c         4280 g         5130 t
ORIGIN      
        1 gttcacatcg aatacgaaat cgccgagagc cttgtggtaa ttgatcagga agtcgctgtt
       61 ccagccatag gaggaggagt tgtattttcc atagcccccg ttgattgcca ctgtgtatgt
      121 gtcattgtac catttttcct ccctttgggt gaagctggca tcgagggccg acctgcccat
      181 tattgaaaga tccttcgtaa tctgatattt aaggctgacc atacctatcg gcctccacct
      241 gtcctgcggc ttgagaaccc ggtttcttgt ccagtaggca ttgccggtgc cgttgtcgtt
      301 ccacctccag taatgctgtc ttacctgccc tgccggtgtg acatactcaa agtgctgcag
      361 gtcctcggtg cggatgtttc tcgggagaac atacaggtat ctcattgggt tgtcgaaacc
      421 ttcacctgta gcgaatatat tttcaaagct ctgctttata accgttacct tggaatcgag
      481 ggtgaatttt tcgccgagct ttgctccgaa cctgaggttc aggttatgac tgtggaggtt
      541 atttgaagca acaatacccg atgcatttgt attggtatag gaaaaagccg tatttgtcct
      601 ggcggtgttt attgtcacct gcaggtttgt tgcaatgctg tttccggtct ggaaatagtc
      661 cttgacgttg ttgggctgcg gaagatacgg atatgtttta ccgtacaggg gatgattggg
      721 gtcgggtgtc cagtgggcga cctgtgagcc gtccattttc gggccccatg aaacgattgc
      781 aagaggtgaa tatacaccgt tgccgccctg tccgtactca ttctgcattt tatcaagcag
      841 gatggccgat gttccctggt aggtaaaacc gaggtcgacc ataacgcctt ctgcccctcc
      901 ctgtccggat tttgttgtta cgattatcac accgttgttc gcacggcttc cgtaaagagc
      961 tgctgcattt gcgcctttaa gtacggagat tgatactatg tcatcaggcg caaggtttga
     1021 aattccgccc ataggtacac catcgatcac ataaagcggc tggctgcttc cggcaattga
     1081 acgattacct ctcataagca cctttgcgtc tgcaccgact ccggtacctg cagttgtcac
     1141 tgacaggccg gcaaccctgc cgctgaggcc tgaaacaggg ttaaggtccc ttgcttcagc
     1201 cagtgccttg ggtgccacat tctgggctgc atatgtaagc gctttggcct gccgcgaaat
     1261 gccgaaagca gtaacaacaa catcttcaag ggcgaggatg ttttgttcca gcacgatgtc
     1321 aaagtgtgtc tgggtgccga tcggaacctc aactgtcttc atcccgataa aggagaccac
     1381 aagggcagct gcattagcag gcaccgacaa tgtgaacgcc ccgtcgatgt cagtcgcagt
     1441 tcctattgtt gtccctttga ctacaacagg taccccggcc ataccaagcc cgtcttcggc
     1501 actggtaact ttacccgtga tggttttttg tgcgaaagcg ccctgcaatc ccgcaaatag
     1561 cagaaatgca agaagaattg caatttttct catggtttaa gattaggtta ataatagttt
     1621 gtggttgcta acattcagat taattaaaaa acccttgttt ccttcccgga atacaatcat
     1681 ttcttaaaga atcgttaaca aaattaaaat tattccaaat aaaaaccaaa agaaaagtta
     1741 attttttcgg agataaatgt taaattaaat ccatgcatgg tatggacaaa tagccggcaa
     1801 tcaatccggc aggattaatt tttaaaaaat aacatactga ttatccaatt gttctaaacc
     1861 agatatggct gaaagaatat ttttcttaag aattctttaa atattttaac attttgttaa
     1921 aatttcccct tatatgtgca gtcggacatt cgggcattgc tttttctata ctttccgcca
     1981 cagaatccct ttctgagggc agaaaccatg aaacattaac ggcaagggac tgttcgagcc
     2041 tgtcactcag cctgtcgaga tcagcatcgc tgtacacttc cgactttacc tgctcatcaa
     2101 gcagatggtc aaccaggttc aggtagtttc tcttgacggt tttcaaacca tattgaagat
     2161 ccctgtcttc ttcgtcgtca gaatccatta tctgccaggc aagctcaagc atcctgtcat
     2221 gaacgagaag accgaataaa ttaatatcgg ccagttcatc gctataataa ttccttacat
     2281 ccgtctcaac cgaataaatg ctctccagct cactgtaaaa ccgggtaaaa tgctggatta
     2341 tctgagccct tccatgcagc ggcatgcttt tgtcatacat gaatgagaga ttatccccgc
     2401 tgacaaggcg ggcaaaaacg gctcctgatt ttttgcttga tttcctgggg agtgaattcg
     2461 gaaagttagt cttcagggta cttaatgtga tgttggcatt tatgtaatcc tgaaggttcc
     2521 ataatctgat gtgatcaggc aatccgagtt tctgatattc ttccggccgg agagacaggt
     2581 cctgcgggct gtcattcctg catgaaaata aaaacaatat cacaaggatc agggcagcag
     2641 agatgttatt gtatctgtac ataatcattt tttaaattta cgagtcgaat attcactaat
     2701 ttatttcaat tattctgtta gttcacccga caggagcatc ttagcatgat tatcatagct
     2761 gttctgtcat ttactttctc gtttgtataa tttcagggaa tcattgttca gtgcaaaaac
     2821 tatgagtttg tttccgtgag ctgtccttat ttcagtcaca tccctggcct caccgcggat
     2881 gaataatccg ctgcgttgat cattcagtgc agtgaagccg ccttttccgt cacctctgag
     2941 gagcagaccc tttcccgcat catagtcgct gaattttatt ctcgacccga agaaattgcc
     3001 ggccagcacg atatcggtgt ttccgtcgct gtcgaaatcg cccgtacaga cgccgtaaac
     3061 gggaaaatac tgtgcttccc ggggcaaagg tctgatttca aaccggtcat tgcccttgtt
     3121 ctcaatatag cagcttgaaa atgtctttgc ctcaaagatt tctgccccgc ttagtgcatc
     3181 cccggaaaag atgtcggtta ttgtaagatt ggcgtaggat tcataggtgg ggtatttttg
     3241 cttaatgaag ggcatctgtt tctgcagatc atccttcgaa tagaaaggat agcttttgcc
     3301 ttgaatgaaa taactcatta ttgcatctat ggtgccgttg ttgtcaaaat cccttgcata
     3361 aatcgtacaa ggttcggcgg gcgaaacctt tatacgtgaa ttaaggccca ggttacctgc
     3421 aatatagtca tcatccccgt cattgtctaa atctgcggca acaattgtgt tccaccatcc
     3481 ctcagtgttt tccggcccgc acaggctggt tatctcttca agccttttgc cttcattcct
     3541 gaagaacctg atcttcatcc attccccgac aacaataaga tcaggtttgc cgtcgcggtt
     3601 gaaatcggtc cagaccgcat ccgtcaccat acccgggtac atcagttccg aattatatgc
     3661 atcggtgaca tccttaaaga aacccttgcc gtcattttcc agtatgtagc tcctgggggc
     3721 aaaaggatac cgggacggaa taagcctacc gccgacgaac agatcgatgt cgccatctcc
     3781 atcaatatca cagcttttta cacatgatcc gcttgtaatc atgacgggca ggcgtccctt
     3841 tgcagccgaa aaagatccct tcccgtcgtt caggtagagc ctgtcctgaa gctcagctgc
     3901 atctgcatga aattcattgc ctccgcttac aacatacaga tccaggtcat tatcgccatc
     3961 tgcgtcgaga aaaacggcac cgatatcttc gcaaccctta tctttctcaa aacaggcctg
     4021 aggaacatac ctgaacgaac cgtcacctga ctggatatag agtttccccg ggcagcccat
     4081 ggaaccgccg aaatacaggt cttcaagtcc gtcattgttc acatcccctt ttgcaatacg
     4141 ggggccctgt gttgaaagga agtgcggcaa cagggcctga tccgcgaaat cattatattc
     4201 attctcctgg tgtttgaaat caaggatcct gttgtttttc accatggtga aaactggttc
     4261 cggctgtgaa ctaatccgcg gggcagggat catggcatca tcgttgttca aaacgatgca
     4321 ctggtcagct tttacagcgg tcagcacctg ctcacgcata tcgggccatc tgatccgaag
     4381 agtgtctgca attgctgctc cgcccagacc aaaaatgatc tcatggttca ctgatgacat
     4441 aaagcccctc gaaggcataa attcctgggt atacgtattt ttaccgcagg tgacgtcgat
     4501 ccgtgatcca atgccgcctg tgttcatccc tgaacccttc agcctgatct tcagataatg
     4561 gttcctgctg attttttcag agttattgcg gtacaccagg ggaaaaccat ttattgtact
     4621 gatcaccagg tctatatcgc cgtcattatc aagatctgcg taagctgccc cgcccgaaag
     4681 tgtagctttt tcatcgcccc agatcccggt aacatcagta aaggtatagt cgccattgtt
     4741 cctgtagatg taattattga gaacggtggg cggtatctga tcaatgacgt tcatcttcga
     4801 aagatgcgtc tgtcccctct gctgcctgac catctggctc acggccagca ccactgcatc
     4861 catataggta ctgttcctcc cgtatccgtt tgttatataa agatctttgt aaccgtcatt
     4921 gtcaaagtca cagaaaaggg gcgcccagct ccagtttgtt gcaaaaactc cggcatactg
     4981 tcctatttcg gtaaaataat caccatgcat gttcagctga agcatgttcc tggtcgactg
     5041 attaaagaat cctgattttt tcaggttctg gaatttttcg tagttgtccg gtcctgccac
     5101 cagcttattt ccgtaattat cctccggttc catatcgagg gtaaacaggt cgataaaccc
     5161 gtcattgttg aaatcagctg catcacatcc cattgaagag aatgaaatat gatcgaagta
     5221 atcttcaagc ttttcagaga aagtcccgtc cctgttatta atataaaagt agtcctgttc
     5281 ggaataatca ttgcaaatgt atatatccgg ccacccgtca ttgttaaaat cagcaattgc
     5341 taagcccagt ccgaaattaa tgatattgcc gatcattccg gcctcagcgc ttacatcaac
     5401 aaacttctcc cctgtatttt tgtaaagccg gtgttcatat atgggatttc tttccttttt
     5461 gcgcgcaggg tttgtcattg cgtacttgtc gagcgaatgg ttcatgacaa aaaggtcgag
     5521 atccccgtcc ttgtcatagt caaagaatgc actgtgcgtt gaataagaag catcgttaat
     5581 tccgtaacgg gcagcacatt ccgtaaaagt aagatcgccg ttattcaggt acagcaggtt
     5641 tttcctgcgg tcatacctgc cgtcggtcga gcagcataca tagatgtcga gaaatccgtc
     5701 accattgata tccgccatgg tggccccggt gttccacttg ttacccccgt tcacacccga
     5761 cacatccgtg atatcccgga acctgaagtt tcccatgttg aggtagagcc ttgcatgagc
     5821 aaaattcccg ctgaaaaaaa tatccggcag tccgtcattg tttatatccc cgattgccac
     5881 gccggctccg ttgtacaggt atccgtagtc aagcacgtta aatacatcat cctcctctat
     5941 ccagttccgg aattttatcc cggtcctttc aggcggcatg gatgtaaaca gcctgtcatt
     6001 ctttctgccg catgaaaact gcaaaatcgt tacagcgaca gctgctgcag caatgagaat
     6061 gtatttgcct gtatgtaatg agcgcaataa catcagcaag gtgcgattgt ggttcaaaaa
     6121 ggttcacaaa tttattcaaa tgttctttta aagcatggca taaatgaaaa atcttggtac
     6181 gggcctgcac aattccattc attcagctgc ggcaaccagt gtaaccagcc attttcaaac
     6241 ggttacaaat ggacataaat gccttgcaag catatataaa tatttaagga ccgatactcc
     6301 aggatcagcc tgtttaactt tgctgcagta tataattata cattcaatta ataaatacat
     6361 gcgatgaatt cactcaagaa cagggtcaac ctgatcggca acctgggtca ggacccggaa
     6421 acaaagaacc tggaaaacgg gaagaaagtg acccgtttta cccttgccac cagtgatgaa
     6481 tacaggaatg ctgaaggaca gaaggtaaag gaaacgacct ggcataacat tgtagcatgg
     6541 aacggtctgg cggatattgc tggcagatat ctgaagaaag gtgcccagat tgctgtagag
     6601 gggaggatcg tttacaggaa ttatgaagac aaaaacgggg caaccaggta tgttaccgaa
     6661 gttgtcatca acgagatgct gatgctgcgg tcgcagaagg ctcagggcag ggaggaatag
     6721 ggaaatcctg aactttttcg ctgcatttcc cgtgcaggct gaagcctgaa tttgattaac
     6781 cgccatactg acagaaatct gatgcaacaa ttctgctacg gtggcggttg atcattcagg
     6841 caataaatac tgtgagtcgt tcaggcaatc gcagttcaga attttctgcc catcctgaga
     6901 gcttctagat ttatattgat aatttcctct cctttccgcc tgaaaagtat tctgacagca
     6961 ttctcaaggc tttcgaatgg cattttaatg aaaggggaag cagcccccag gatcaccata
     7021 tttgcagctc tgaccgatcc ggcttcccgt gcaattgcat cggcatcgat gatcctgtgc
     7081 ttcgggagtt ttctgatttc atgcagaata tcttccagcg gagggtaatc cggtatattg
     7141 acaaatggtt ccgagctggt aatgagccat ccgtctgaag agagccaggg caggtacctg
     7201 agtgattcca tcggttcggc agaaatgata atgtcggcct gcccccaggg gatcagatcg
     7261 gaatgaaccg gtttgtcgga aatacgcacg tgtgactgca catctcctcc ccgctggctc
     7321 attccatgaa cctccgcctg tttgatatga aggttgttct caattacggc caggccgatg
     7381 atggcagcaa tcgaaaggat accctgtccg cctactcccg aaagtatgat atcgttcttc
     7441 attgttcagc ctccccggga tcattaactt ccagcgattc tgccttcctg cgccgcgaag
     7501 ctgtctgaat gcattcccgg ctggaaataa taactgaaac attcctgtaa ctgaattcct
     7561 cctgcataat cctgacattt tccgcgtgat tcttcctcag cggtacgatc tcgcgcacat
     7621 gttcgggggc aacccccagt ccttcgcata tttttctcag ccgtcctgta ccctgtgatt
     7681 tctgacctcc ggtcatgccc gttgttgagt tatcggaaat gattattgta accgggcttt
     7741 tttttacaac cgcatcgagc agaccggtca ttcccgaatg tgtgaatgtt gaatccccta
     7801 tgacggctac cgaggggaac agtcctgcat cggcagcacc aatggccata gttaccgatg
     7861 cgcccatgtc aacgcacgaa ttaatgatac tgtatggagg gagggctgcc agggtgtagc
     7921 atccgatatc tgagaacact ccgtccctgt caaatggccg gagggcttct atcactgcat
     7981 tgaaggtgtc agcatgaccg catcctttgc agaatgacgg cggtcttggc ctgaccacgc
     8041 cgggaatttc aaatcccttt ctgatagtca taccgagggc ttttgccacg agcgtaggat
     8101 ttagctcccc atcgcgcggc agagtaccgt caaggcgccc cattatcttc agattgttgt
     8161 cgagaatacc acgtaaggcc tcttccacaa gcggatatcc ttcctccagt accagaatct
     8221 cctcgcaacg tgatgtcaga tccgttataa gctccttcgg gaaaggatat gcacagatcc
     8281 tcaggaatgg aaaatcaggc tcgctgtccg gaaaattctc ggtaaaatag ttataggcca
     8341 gtccacaggc cagaaccccg gtttttctgt tcctgtcatc cctgaaaatg ttatgccggg
     8401 aaagcctgtt atcgtatttt aatgactcat aaatctccag cagcgccctg tatttttttc
     8461 ttgcaatggc cggaagaagc acaaactgcc gcggatcgga aggcaggctg agctttttct
     8521 gaggttcgga ctgccgtctt gtcacggctg acctggagtg tgccagacgg gttgttatac
     8581 ggaaaagaac aggcagcctg tatttttcgg aaagttcaaa cgcatcaaac accatgtcat
     8641 atgcttcctg ctggtttgag ggctcataaa ccgggacaag ggagaatttt ccgtaaaacc
     8701 gtgaatcctg ctcattttgc gatgaatgca ttgaaggatc gtcggcagca acaactatca
     8761 gccctccatt taccccggta accgaagaat ttataaaagg atcggcagcc acattcagtc
     8821 cgacatgctt cattgcaacc attgcacgtt tgcctgcata tgacatacca agcgccgctt
     8881 ccatggcagt tttttcgttt gacgaccatt ccctgtggat catacgttca gcggcctcat
     8941 ccgatgcctg tatgaattcc attatctcgg tcgatggagt tccgggatag gcatacattc
     9001 cactcaggcc ggcatcaatt gcagcctgtg caatcgcttc gtcacccagc agcagttgct
     9061 tttgcatatg taaagtatca attacaaacg accggacaaa tttatcagat aaagtttaat
     9121 tttgaaattt ctttaaaatt aagccaaaag ttttactgtt ctgtccaata ttgcttattt
     9181 tgcaggcgga tggttaatat gtcagggaat tagataattt gagttggcaa aacgtttagc
     9241 aaatacaaca tttttatttt tcatcttttt tttagcaacc aggggtcagg cggttcagaa
     9301 ggaacgattt tttcagctga gcggcataat tactgatgaa aacaaccggg ctctgagcaa
     9361 tgtgggtgta atatccctcc gtctcaagag aggagtctta agtgagaata ccggtattta
     9421 ttccattacc agtaccccgg gcgacacaat attattcaag gctcttggtt ttaaaaaaca
     9481 gatgattgta ctgcccatgt catttgaagg caagcgttat tatgccgaca ttgttctaag
     9541 tatggatacc attccgattg agaatgttgt tgttttaccc tggaagagtt ataatgagtt
     9601 tatcagggat attacatcgc ccaatcccgt cgatccggaa attgaaaaca tgaacaaaaa
     9661 catagagtcc gtcaaatctg cccttgcgaa ccaggctgat gtccgcatca gccctgaagc
     9721 aggctacagg tatgcaatgc agcagaattt caatatgacg gcaacccgga accagtatcc
     9781 ttcgaataat cttctgaatc cctttgcatg ggctaaattc attaatgggc tgaaaagcgg
     9841 tctgttaaaa aatgacaagg ccgaaaagcc gtccaggaca aaggttaaaa ccaagaagaa
     9901 aaagaagcag tgacgaatta tcatgacaaa atagccggtc tgcttggaac tgtgatcctg
     9961 catctgattg caggaattat ctttgtttcg ttcaaacttc atacattaaa ggtcaggcag
    10021 gaaaccattc aaagacttga acttgaaatg gttgatgagc atgacctcaa accaagggaa
    10081 gctccgaagg aaattcctat gacatcggta gaaaatattt tccgtgatga tgcggaaatg
    10141 ctcaatatcg ccaggaacct ggccagccgg agtgagataa aaatagacag gaatgattat
    10201 atcgacaagg taaaggaaga gcttattcag agtggcaggc tcgggaccga caattatatc
    10261 gataatcccg ggtcaggtta ccaggaggcg gaagaggata tttccaacgg ggacgatgaa
    10321 gaagccttac gggcaaagga agaagagaaa gtcatggagt cccaaaaaat ggccgccagt
    10381 tattcaggcc ccacccggat ttattatgat ctggcaggac gcaatcatat ttatctgcct
    10441 attccgatat ataaatgcca gggttccgga aaagtagtgc tcattatcga tgttgatcag
    10501 aaaggcaatg tcaccagtgc atctgttgtt gccggggaaa gtacaacctc agatgaatgc
    10561 cttaccgaaa ctgcagtcac atctgccagg aggtcgaggt tcaatgccga tctgaatgcc
    10621 cccaaatcgc agaggggaac gcttaccttt atttttgtag cccagtagct gatagtaaaa
    10681 gatgcaggga tgcagggaat gaaccatgcc cctgccagcg cacggttcag aacggaaggg
    10741 ttctgtcagg atagtcgaac gggcggataa tctctgatgt attcctgtca gctgatttgc
    10801 tgttaatgag cctgcttact gtatgagcct tcagcccttc ggaaggatac ggtttcagca
    10861 gtgaaatgag ctctgcctca gtcaaaccgg cgttgagcca taattcttca tcctttttat
    10921 caagaatggc cggcatcctt ttcctggaat tgtggatcac tgccatcatt tcattggcgc
    10981 tggtagtaat cactgaaaaa gattcagtaa cattaccggt taaactatcc gtccattgat
    11041 cataaatacc tgcaagggaa aagatgtcgt tatcagaatg atagatatac catgggattt
    11101 ttccgccggg tgtatgctgc cattcgaaaa agcccctcac gggcaccagg catctttttg
    11161 attttgcagg attcgagaat gaaggttttg aaaaaaggct ttcggccctt gcattgaatg
    11221 tcttgtatct gatctcctcc gcatctgccg cggattttgt ccagaaaggg atcagccccc
    11281 atttcatcag ttctatcctg ccgggtgatc ccgtgcacac aacaggcagt tcgggaagtg
    11341 tgaaagcatg ataatagtaa ctgggccgga acctgtcagc atcgggaaaa agcacgccga
    11401 acctgctttc aagttcatcc ttaaccaggt tcacattgac agtaaaacac attattcata
    11461 ttttggagca ggaacgtcaa ccttcaccgg aaccggcagg gagtattcaa acaggtcgtt
    11521 ttccggccat tcgtctgtcc gcgcaatgaa ttttgccatc atcctgttta tgtgggaatg
    11581 aatctcaggt ccgttccagg cgcttgaatt gagcaaaatt acccatgaaa tgccatcagg
    11641 ctgccgcttc atcattcccg ctgaaccgga aaaggaaccg gtcctccacc agttgccgtc
    11701 aattaatgta gccttccatc cgacaggtgc gaatcggttg gccatatctg tcatgaatct
    11761 tatgctgctg tttgacaaaa tatcttcagg ggcaacaaat ccgtcgacag ccaggagcag
    11821 ccgcattaga tcgggtgcag ttgccagcca gtagccggct ccacccagtg tctcaacatc
    11881 attccccccg tatctcacag gtaccagttc atcagtggcg tagattgagg gtccgggagg
    11941 agtcccgaaa ggttcgtaat aggtaacttc aaacctggct ttctgcgatt ccaggttcct
    12001 tgccggaagc atgtcataaa taccaagcgg ttcaaaaatc tcttccctgc aataggagtc
    12061 gtatgtcatt cccgaaactt tttcaacaac aaggccaagg attgcataac ccaggtttga
    12121 atacgaccgg ccggtccctg gagtgaaatg tagccgcttt ccaagtgcga accttatgat
    12181 ggtattcaga tctaccggaa gcgggacatt catctgtttc gaaaccaccc gtggcatgaa
    12241 catctggtcg ccatactttt gtgaccatcc gccttcatgg ctaagcagat gggcaaccgt
    12301 gatgtcaaaa acccttttat cttttgggta gcaatagcag gaatcgttaa gaataccgtc
    12361 cggtccgaaa accctgtcgg aaagatccag tcttccctcc tcatgcagct tcataattgc
    12421 tacagcagtc accagttttg aaatgcttgc aatcctgaat ttactgtaag gctgtacctc
    12481 ggaagccgaa gcagtgtcgg caaagccgaa tccccgcgcg taagccagtt taccatccct
    12541 ggcgacagcc agggatgctc cctgaatctt ccatttgtcc atgaatgacc ggattatcca
    12601 gtcattctta ccaagcacat tttcaaatga atcggaattg gtgagccgga tgttaccaac
    12661 tgtaagttta tcgcgcacat cgccggggtt ttcaacagct ccagggtcat gtgccatgaa
    12721 aacggcaaag aatatgatca atattatctt tttcaatccg ggcagacaaa caggttttct
    12781 cacgtcaaaa ataaaatttc ttctacggcc tgggttatta aataatttca aaaataatga
    12841 caacattgaa atgaacaaat atgcggttaa atttcaaaat tataattttt caaagcctca
    12901 aaacaactaa ctttgtgcac atgttgtcaa caaaactgat attaagacat ttccggacca
    12961 ttcttctttc attgctctgc agtggtccct tgcatgcaca aagatccagt attgatacaa
    13021 ctgattatct gccatattat tatgaccagg ctgcagaatt caatctgata attgcagcat
    13081 caaaaggtta cagcagtgaa attgacaggc tcatcagcaa gggagcgaat ccggaggctg
    13141 aaaccatcga gggtgcaact cctctcatct atgccgttgc caacaatcat acggatgcgg
    13201 taaagtcgct gctggcacac ggggtcgatg taaataaaac aacggaaaac aacctgacgc
    13261 ccctgcttat ttcagtaaaa aaccagaata ttgaaatatc cgaagccctt ataagggctg
    13321 gagcagaaat taacaaaaca gaccggtacg gagctgctcc cctgcattac gccgtgatat
    13381 acggtggttt ttatatcact gatctcctgc tttattacga agctgactgc aatcttaaag
    13441 cgggcgacgg taccaccccg ctgatggctg caatatgggc cggatatgct gatatagccg
    13501 atctgctcat tcagaacgga gccaacatgg aagcccggga taaagacgga ttcaccccgt
    13561 ttctcattgc ggcccagaac ggcgatactc tcctgatgaa cctgcttttg aaatacggag
    13621 ttgacctgta tgaaaagaac gctttcaact tcgatgcact gggcattgcc atcgaatcga
    13681 atcagaaacc tgccgtagag atgctcctgt cccgcggaaa cgagtggaac tcgggtgatc
    13741 attcaggtat taacccttac cagattgctg ctgcatcggg aagaaaggaa ttcagggata
    13801 tgcttgaaaa gaaaaatgtt ccgggcagaa tacaaccgtc aataagtgaa atggcagtgc
    13861 ttctttcaac cagggttacc ggcggaggat tattcaccgg agtcagcctt ctgttcaggg
    13921 atcccatgac agaggcaggt tttatggccg gattcgacat aaaaccctgg tattcaagag
    13981 taatgatcca ggataatgag accctttttt accagtaccg tgacaggagc gcattggcat
    14041 atgcaggaat tttcaaggat ttcagactga caggtgatta caggaagacc aggctgatgc
    14101 taactgcctc catatctgct gcctatacat tcggcaacaa actaaggggc acaaatattg
    14161 aaccggaaag caaattcaga ctgaaccccg gagctggaat taaattacag aaaaaacgcc
    14221 tgatgctgac agccggtctg gaatatctta attcgcccta ttacagggtc ggccctgtat
    14281 ggctgaggac aggcctggcc tggaacatcc agttcagaaa aaccagatca cccggaaaaa
    14341 caattaaatg gtattgatcc gcatgaaaga ggcaatcaga atctttgcaa ttatactgat
    14401 ggtattatgt ttttcctgcg agggcagcaa atggctcgtt aattgtgaag actgctacag
    14461 cgaagaaccg gttgagacag ttgtcagaat aaagctcagg gacattcagg cgcctgttac
    14521 cattaaaatt tacgacggtg acctggaaga caatattatc ctgacctcat ttgaggcata
    14581 tggttcagaa tacaccgtca ataccggaat aaataaaaaa ctgaccttca ccgccacata
    14641 caacatttcc ggcagggttt acatcgccgt cgattcaacg gttccccgcg tgaagtacga
    14701 gaaagatctt tgcgacaatc cctgttatta cgtttatgac aatatcgttg acctccgtct
    14761 caggagaacg gttcccggca gctagacagc cagatttccg gatcatgcat cctcattgcg
    14821 gaaaatggga actccggcaa gtcctttttt cctgatctct gctacttcct cgttacttaa
    14881 cgggccgagt tcgtttctca tttccagtac gagtttatag agttccgtct ggcccgaagg
    14941 tacgacagct gtcaccggat gtgacagtgc aaaacgcaac cccatccgta tttcatcagg
    15001 ggttgaaagc ggttcatacc agcatttcgg atatttggac cggtcggctc catccttcca
    15061 gggacccctg gccatggcct tcagagccag aattccgagg tttttctgat gagccttttc
    15121 cataacctgc gggccgaaat taccggcata ccaggttgca tagttaaacg ggaagagaat
    15181 ggtgtcaaaa tcaaaacgat ccatcagtgc catggcagcc tgtaccgaat gggctgtgaa
    15241 accgataaat ctgatcttgc cttctttccg tgcttcaata aaagtttcca tggcccctcc
    15301 cgggccgaga agccggtcaa catcttccat cagtgtaacg gcatggtgct gataaaggtc
    15361 aaaatgatcc gtacggagat tcttcaatga ttgttccagt tccttacgtg caccctccct
    15421 ggtcctttca gtggttttgc atgcgaggaa cacatccttt ctgtatggtt caagagccgg
    15481 accaagctta atttcagcat ccccgtaagt tggagccacg tcaaagtagt taatgcccga
    15541 gcttatagcc atttttacaa gtaaggaggc atcttcatgt gattcatcca tgacaacaac
    15601 tcctccaaat cccagcactg aaagcatttc tccggtcctc cccagcgccc ttttttctat
    15661 tttacccggc actgacaacc tggagaggcc tgaaagatca gccgggaaca tggatattat
    15721 aaggtgctgc tgccgatgca gttttgataa aatcacgtcg tttcatctgg tcagttatta
    15781 tgtttcaacc ataaaagata cgaattcttt ttctggtatc tgtcaaacca ggcattaatt
    15841 tttcataccg cagtgaaaaa tcaaaatgac gatcctgcgg attatttatc cgaaaaaaaa
    15901 ccttacatta agtatcttta actttggcaa aaactctggg atatggaaac ttcattcttc
    15961 aactcggcaa ccttcaatta cattatcata ccggtaatga tcttccttgc aaggatatgt
    16021 gatgtatcga ttgggaccat gcggataata tttgtctcaa agggcaagaa aaacatcgcc
    16081 cctgtccttg gcttttttga agtactcata tggatcactg ccataagtaa aataatgcag
    16141 aacctcgaca attacataaa ttatgtagcc tatgcggcag gctttgccac aggaaatttc
    16201 ataggtatga tcattgagga gaagcttgca atgggaatcc agatgatcag ggtttttgct
    16261 cacgaacgcg gcagggacct tgttcagata cttaacagca atggttttgg tgccacatcg
    16321 gtggaggccc atggtgcgat agagaaggtt catcttgtat attccattgt ccacaggcat
    16381 gaacttgcaa acgtgcttga gctgatcaat gaattcaatc ccaaggcgtt ttttacgatt
    16441 gaagatgtaa agtcggttaa tgaaggtatt ttcacaccaa gaaaaccggc caccatactg
    16501 ccattttcaa atatcctgag ggaatggcgg aaagggaaat gaaagtttat atttttgctt
    16561 aaagatttca cattattcag catgaatgat ttcagcaggc atccgctata caggatccat
    16621 actcttaaca gtgccatgag ttcaatgtgg gcattttaca gaaagaattt cacggtgctt
    16681 ttcatcatgt cgcttgcagt gtcgctgatg actcagtttg tttcgacccg gatagatttt
    16741 gcagggatgc agacaatcac ggatccgcag gaaatgctgt cagaaatgag aggttatgct
    16801 gccccgttgc tgctgatagc aattctcaat ctcctgttca ccacagtgct ccaatactat
    16861 gttatatata atcctgttga cgaaagcaat aatattgtca gatccctggt cagttcccta
    16921 cggtatttcg ttccgtacat aattatactc atactcctgg catttttcgg gtcaatgctc
    16981 attgtgctgg gtctggcggc ccttattgtg ggcgcgtttc ttgctgccct atatgtattt
    17041 accgtttacc ttttcatttt accggttatg atgtctgaag gtcccagtat tgcaaataca
    17101 atttcacgaa ccataaggct ggcacacagg aatttctgga ccaatgccgg ctggacggca
    17161 gtatttctcg tcattctgat tgttgtaacc gtcatttttt caggtctgat cctgcttccc
    17221 ttttccggaa gctttctgaa ggtatttacc aatcctgaag ctgccggagc cgttgcagaa
    17281 cttgcctcaa atcctctcta ctttatcctg actgctgttg tcaatgccct ggtgttcccc
    17341 ctgatgccgg tgtttgcctg tattctgtat ttcaatggcc gggcagggga agatcaaatt
    17401 tccgatgccg gtgcgaatcg tgatgaagac aacggaagga acgttaggat tgaagatctg
    17461 tatgcaaggc cttcacctga ggaggataca gctgccggcg agaaggaata agcacccttc
    17521 agctgaagaa caaccgatac ctgggaaatt accgtatcaa aaaaagaagc aaaaaatcca
    17581 aacctgaagt aagatgaaaa acgcagcagt tgtcggattc ggattcatgg gaatgactca
    17641 tgccaggaac ataatgaagc tcagggatct ccatctttcg gccatcgttg atcccaatcc
    17701 tgccctgttc agcggtactg cgcccgtaaa atcgggcaac atcaacactg gcgattctcc
    17761 ggcgggggaa tttgaatcag tcagcaaata tgctgatctt gacaaatgcc tggattcaga
    17821 agatattgat gctgtaataa tttgtgtcca tacgggtctg cattacgaaa tggcaatgaa
    17881 agccctgggc aggggcaagc atgtattcgt tgagaagcct ttttgcctgg acatcagtca
    17941 ggcacaggaa ataattgatc tttccaggca gaaggacagg atactgatgg tggggcatgt
    18001 ggtacggttc atgagtccgt acaggctgct gaaagaatgg tcaggcaatg aaacgtttgg
    18061 caagcctgaa ttctttcatt tctggaggtt ctgcggtgtt ccggcatggg ggcagtggaa
    18121 tgaggagcag accgtgaaag cctcgggagg cgcgctgttc gacctggtga tacatgatat
    18181 tgatttcgcc agctacgttc tcggagctcc cgacgatatt agatccgtca gccttgccgg
    18241 ccggcagagc agtcatgatt atatttcggc aatctggaat tacccgtcca gcggaatcaa
    18301 tgtcagaatt gaaggaggga acatattcca tggcagtttt cccttcactg cagggtattc
    18361 cgcccgtttt gaaagggcca gcgtacatta cagcacttcg aacggcaatg aaataattat
    18421 ttcggatgag actgaagtaa aaaaaatacc taccggtgat ccgggagcag gatactatga
    18481 agaaatgtcg tatttttctg actgcattgc aaccaaccgg cagccgtcac tctgtatgcc
    18541 tgaatcatcc ctcggatcag tggcattgtg ttacaggcat atatgatatt taagcagcag
    18601 aggccggcag tacttaagtt gccggcggga tgatcatttt taacggtaat tttatacaaa
    18661 atataaagtt ggttttattg cctggaataa ggtttatgac acgatacttc gtatcaaaat
    18721 cggcaagatc tgtcttcacg cgtccacccg gaccagcggc gatttatgcc ggtaatcttt
    18781 ttcctaattt tatcggtcca ggatcattaa taatgcgaat taagagttaa aaattaagta
    18841 aatgaaagct gctgcgattt tttcaaagac atgcacgagc aaacctttag tagatcgcac
    18901 tttagacgct ctttgaat
//
LOCUS       DAOOYG010000043        18787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2836_length_18787_cov_6.065716, whole genome shotgun sequence.
ACCESSION   DAOOYG010000043 DAOOYG010000000
VERSION     DAOOYG010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18787
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2836_length_18787_cov_6.065716"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PK288_05525"
     CDS             <1..829
                     /locus_tag="PK288_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPM18415.1"
                     /translation="PWIEYRMSADMESILKGYLDPRVTTWFSPALEPDPTDDPVGISF
                     PYEGVRNGQTKNDRSAVEFDRIASDHSKPYTIVGDKGPNWFVMRASETYFLRAEGVLE
                     GWNMGGGTVEELYNTGIGLSLDECGFTDHLNLAGENYITSPRVPMTPGIDKNTVLPAV
                     APPPASTVPILFNTGGSKEEQLEQIITQKWISLFPNSQEAYAERRRTHYPKLYPRLES
                     ENLDVPATSLPVRLCYWTNEYNTNAKMVNAAIQMLNSESTTPNGDKASTKLWWDKKP"
     gene            919..1284
                     /locus_tag="PK288_05530"
     CDS             919..1284
                     /locus_tag="PK288_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18416.1"
                     /translation="MERKCLDCGDIIRGRSDKKFCSDQCRNSYNNRLNRDTNNFVRNV
                     HGLLRKNRRILADLYTEGRRRTHKDALFALGYNFSFFTHVIETSGSEKYLFCFEYGFR
                     DAGDDFIELKYNNQYIDYQ"
     gene            complement(1278..2165)
                     /locus_tag="PK288_05535"
     CDS             complement(1278..2165)
                     /locus_tag="PK288_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPM18417.1"
                     /translation="MKGITLFLQIFLLLFIPLSVKGQQTDSGKKLHIIIIGAHPDDPD
                     KVGGTAYKWARLGHDVLMVSLTNGDAGHQSIPPDKLAAIRREEARKAGQVIGVKYITL
                     DNHDGQLMPTYENRLQVIRLIREHKADVVIFPRPYDYHPDHRYTGTLVLDAAYMVTVP
                     RILPEVPHLDKNPMFLFMSDGFIHPEPFKADVCVDIDDVIEKKIDMYHQHTSQVYEWL
                     PYNRGALAEVPAGERERREWLGRTWSTRSDAEPFRDKLIELYGKAKGSKTKYCEAFQD
                     SGYGTPLTSENLKYYFPFY"
     gene            2581..3201
                     /locus_tag="PK288_05540"
     CDS             2581..3201
                     /locus_tag="PK288_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPM18418.1"
                     /translation="MKKLCLILLVMVMSAPVYSQVGFGVKGGLSTTSLKMDDLKLNTD
                     TTINAIKGANFGYHAGIFLRLGMMGVYVQPELLFTSRTNEYRIEDLENPATIVKQQFN
                     QLDLPVMLGFKAGPVRINAGPSARLLINSPKDLINDPDYKTMIKNFTFGYQAGLGFDI
                     IKKITVDLRYEGSLHKYQTQIQKSTGDKFKLDDRPNAFHLSIGFIF"
     gene            complement(3221..3589)
                     /locus_tag="PK288_05545"
     CDS             complement(3221..3589)
                     /locus_tag="PK288_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPM18419.1"
                     /translation="MTTTSLLLLLAIGLLTGTLAGMLGIGGAIVMIPALVFLMGFSQH
                     SAQGTSLAVMLPPIGILAAYNYYKAGHVNLKFALILAAAFIIGSYFGSKIALNVPQQI
                     LKKIFGVLLLIVAVRMLFVK"
     gene            3709..4737
                     /gene="mltG"
                     /locus_tag="PK288_05550"
     CDS             3709..4737
                     /gene="mltG"
                     /locus_tag="PK288_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPM18420.1"
                     /translation="MRHMRIYILAAILPAVILSGLFCYLVFGVTIRPAGSGSQVIYLP
                     EESTFEQVMDSLKTNLRIDNLKVLQWVAEKKNYRSHIKPGRYVIEKGMSCNELINNLR
                     SGKQTPVLVTFSNIKTIYDLAGRVGGKIEADSARIVTFLSDTENYRSDGFVRENIISV
                     FLPDTYEFFWNTGPGEFYSRMLREYKKFWNAERIRKADEKKLTPLEVSVLASIVDQET
                     SMPDEKPVIAGVYLNRLKRGIPLQADPTIKFVINDPKMSRILRRHLSINSPYNTYKYK
                     GLPPGPISCPAKDGIEAVLNADNHDYLYFVARSDFSGYHVFSRTLSEHNRYAAMYRKE
                     LNRRKIFR"
     gene            complement(4739..5746)
                     /gene="murB"
                     /locus_tag="PK288_05555"
     CDS             complement(4739..5746)
                     /gene="murB"
                     /locus_tag="PK288_05555"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPM18421.1"
                     /translation="MIYENISLKKYNTFGLNVKADRLIPFKHEENVISFLRQQGGSEQ
                     SLFVIGQGSNLLFTGDFHGTLIHPEMEGITLEGRKDDVVIISSGAGVLWDKLVESTIN
                     NGFGGLENLSLIPGSVGAAPVQNIGAYGVEVRNTIERVRAVSLENGSVREFTNDECQF
                     GYRSSIFKGELKGKYLITKVYFRLSTRPKLSLEYGSLREEVSKLGSVSLKNVREIVIN
                     TRKSKLPDPAILGNAGSFFKNPAVSESLAEELKKKDPQLPVFNNDSGEVKIPAGRLIE
                     LCGWKGRRIGDAGVHDRQALVLVNFGRATGKEIFDLSEKIKESVFEKFGIMLESEVEI
                     L"
     gene            5857..6513
                     /locus_tag="PK288_05560"
     CDS             5857..6513
                     /locus_tag="PK288_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPM18422.1"
                     /translation="MNKSSVIKKTFPFLEKGLTEEISNVSEIRDLAAGMSILGEGDYI
                     KSFPMVLAGCLSVVRLSDDGNELLLYYLNQGEICAMALTCCMGPQKSNIRMTAEEDSV
                     ILTVPVEYIEKWMSDYRSWKEFMMYSYRKRFDELIEIIDAIAFMKLDERLIRFFRERF
                     RSTGKTVFTGTHQDIALQLNTSREVITRLLRNLERNNLVITERHFIDYSLLVKKDTSL
                     "
     gene            6569..6793
                     /locus_tag="PK288_05565"
     CDS             6569..6793
                     /locus_tag="PK288_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18423.1"
                     /translation="MTDFIRKNLFVIIFTAAGAAGGFLYWRYVGCLSGTCMIKSVWYM
                     STLYGMAVGWIGGSLLNDLFLKFKKAGKNE"
     gene            6786..7112
                     /gene="trxA"
                     /locus_tag="PK288_05570"
     CDS             6786..7112
                     /gene="trxA"
                     /locus_tag="PK288_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPM18424.1"
                     /translation="MNESFQAIIEGRTPVLVDFSAEWCGPCKMMKPVLEQLKQKMKDR
                     IRIIKIDVDRNRELAMKYRISNVPTLMLFSENRILWSGSGVVTANFLENVINNNCPVP
                     ADQKVN"
     gene            7429..8922
                     /locus_tag="PK288_05575"
     CDS             7429..8922
                     /locus_tag="PK288_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18425.1"
                     /translation="MKSNRRDFLRNSTLAGAGAFVGSFLQGCVPAKEESNLAGIIEAV
                     KKPHTQKFNMSGYAAPPLPVVRVGFIGLGDRGSGAVQRLSYIEGVEIRALCDKREAAV
                     KASQKYLQSVGRPVAEEFFGTEDSWKKLAEMPDIDLIYQCTPWVMHTPVAVYAMENGK
                     HVACEVPIARTIDECWQLVETSEKTRKHCMMLENCCYDFFELLTLNMARQGMFGDIVH
                     GEGAYIHNLVDMIFRKPDDSRQSDNAYTDMWRLKENAERTGNLYPTHGLGPVCQVMNI
                     NRGDKMDYLTSMSTNDFTLGKYASGLAAADDFYKPFVGRPYRGNMNTSLVKTSLGRTI
                     MIQHDISSVRPYSRLHVISGTSGAAIKYPEPERIALGHEWISEKEMEDVIDKFTPEIV
                     KRVGELAKKVGGHGGMDFIMDWRLIDCLRNGLPLDQDVYDGALWSAIAPLSEWSVANR
                     SQSIDVPDFTGGSWKTNKPHDINLEKGGNTLVLEIKESKPELQLNIS"
     gene            complement(8974..10662)
                     /gene="ggt"
                     /locus_tag="PK288_05580"
     CDS             complement(8974..10662)
                     /gene="ggt"
                     /locus_tag="PK288_05580"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPM18426.1"
                     /translation="MKLQIIVAGLILTNISLYSQDRITGRDFATRSEVIAQNGMAATS
                     QPLATQVALDILKRGGNAIDAAIAANAVLGVVEPTGCGIGGDLFAIIWSAEKGRLYGL
                     NASGKSPRKLRLEYFRNNNIEKIPSYGPLPVSVPGCVDGWFEMHEMFGRLPMKDILQP
                     AINYARNGFPVSELIAYYLDSNTRLLKDYPNIKETYMPGGKSPAKGEIFRNPFLASTL
                     EKIAKGGRNEFYRGSIAKTIDAFMKSQGGFLSYDDLSRHHSEWVEPVSTSYRGYEVWE
                     LPPNGQGIAALQILNILEGFDIASMGFGSSEYIHVFTEAKKLAFEDRARYYADPLFSA
                     VPVSRLISKKYAAERRKLINPGKAGKIFDPGKPEAGNTVYLTVADRSGNMVSLIQSNY
                     RGMGSGMCPPGLGFVLQDRGEMFSLSEGHANVYAPGKRPFHTIIPAFITRKGRPWVSF
                     GVMGGDMQPQGHAQIVVNLIDFKMNLQEAGDAPRIHHIGSSEPTGEQMTDGGILLLEN
                     GFRWEVIQKLLKMGHTIQWDLGGYGGYQAIMLDERNKVWYGASESRKDGQAAGY"
     gene            complement(10679..11980)
                     /locus_tag="PK288_05585"
     CDS             complement(10679..11980)
                     /locus_tag="PK288_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPM18427.1"
                     /translation="MNELIFKSVSEIIGHYHKIYTDLHMNPELSMMEKRTSAIMADNL
                     EKLGFETTRGIGGHGVAGIFVNGNGKTVMLRTDMDALPVKETTGLPYASTVVMKDMNG
                     RETPVMHACGHDLHMSVWLGTLQTLIRMKDHWSGTLLAVAQPAEEGMRGAEAMLRDNL
                     YSRLRVPDCALCYHVSADLPAGSIGYFPGPIFAGVSSAQITVYGIGGHGAMPHNTIDP
                     VVIAARIILDIQTIVSREINPVKPAVITVGAINGGTRANVIPDEVKMLLTIRYFEESV
                     LNTIRESLINITRGAGIAAGLPEDKMPLVTFSSEADLPVSNDPQLVMNCVRSMSDILG
                     GKNLTEVEPATVAEDFGKYGQTAEKVPIALFWLGGVNRQKYDEHIASANPLPSLHNSS
                     FAPDFEPAFTAGVAAMTKSVIDILSNKADSRQKKTDRYSEK"
     gene            complement(12082..12786)
                     /locus_tag="PK288_05590"
     CDS             complement(12082..12786)
                     /locus_tag="PK288_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPM18428.1"
                     /translation="MNTIIEEIRQELVRHSEEAHRLSARGFFREEVRLYGVKSAEVTG
                     IAKRIFKNLTNKSKDNIFSHCEELWKSGMLEESFIACNWSYNVRTEFEPSDIEIFGYW
                     VDRYISNWASCDTFCNHTVGALVEMFPQSIRILKKWTGSANRWMRRASAVSLIIPARK
                     GLYLEEIFEIADILLLDKDDMVQKGYGWMLKSASHLHKMEVFDYVYRNKGIMPRTSLR
                     YAIEKMPRELRIKAME"
     gene            complement(12956..14197)
                     /locus_tag="PK288_05595"
     CDS             complement(12956..14197)
                     /locus_tag="PK288_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPM18429.1"
                     /translation="MIILFLTSGLFLGWSLGANDAANVFGSAVGSKMISFWKAAIIAS
                     VFVILGAVFQGSGTSETLSKLGAVNALGGSFTVALAAAVTVYLMVKFSIPVSSTQSIV
                     GAIIGWNLFTGNTTDRSVLTQIVTTWVAGPVLGAIFAVILVSILMKIKKKAGIHLIRF
                     ESYIRTSLIIAGAFGAYSLGANNIANVMGVFVPAFNLQELSFGFFTLSSPQQLFLLGG
                     LAIAAGILSYSGKVMQVVGSSIVELSSEAALVVVLSQGIVLFLFSSSGISEFLKNNGL
                     FPLPLVPVSSTQVVIGSIIGIGFYKGARNINFRLLGSIGAGWLITPVFSGLLACLTLY
                     FVKNVFSVDVGSRNSTGTISGITEGSANISDASASGILGYFIIGVLIVGVISLLLYYI
                     LERKKKIEFQRSEEKFWKYLK"
     gene            complement(14341..14991)
                     /locus_tag="PK288_05600"
     CDS             complement(14341..14991)
                     /locus_tag="PK288_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPM18430.1"
                     /translation="MPVFKRTTKNLILKIDEFFDNVDLGLLVFREAVKDYLQKDMEAF
                     ERHVQKVGSLESTADRLQRSIENEMITHSILPQHRGEVITLIDVMDEILDTIKSSLDE
                     FSIELPDIPPSLNRDFLNITEASVSAGEELVPAARAYFKAPYTVREKLLKVYYFESET
                     DKITKSTKKIIFQEMKELELAHKNHLRYIINRIGDISDNAQKAADLLSAMSIKILM"
     gene            complement(15028..16398)
                     /locus_tag="PK288_05605"
     CDS             complement(15028..16398)
                     /locus_tag="PK288_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPM18431.1"
                     /translation="MYAIVDIETTGGSAKNERITEIAIYQHDGSKITDEFISLVNPER
                     NIPYYITSLTGITNEMVNEAPRFFEIAKKIIEFTGGRIIVAHNAKFDYSFLRQEFSSL
                     GYNFRRQVLDTVSLSRKLIPGYESYNLGSICRHIGIEINGRHRAAGDAMATARLFERL
                     IEIDRELTGKSSRLISNTKISKLNSSLDTSKLDKTPEETGIYYFYNERGDLIYIGKSR
                     NIKQRIATHLSNNTSERSMAIRDKIADFSWEITGSELIALIKESYEIKYNKPVYNKAQ
                     RNTEQRWGIFCFRDDKEYMNFSAGRIAGNDIPVAIFASKERARSRLAELVVKYGLCQK
                     LTGLYNTSGPCFHYHVGVCRGACCGKEPAASYNERAKKASEEFVFSRKNYFIIDRGRS
                     SDERSAVKIVNGRFTGYGYFNINEMGFGLTALHDCIMDCQDNRDIQLILKQYLRGYKV
                     DRIIEF"
     gene            16496..17326
                     /locus_tag="PK288_05610"
     CDS             16496..17326
                     /locus_tag="PK288_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPM18432.1"
                     /translation="MNECFGRKFVLNGSLMPAELFDNSMVYEGDSVYEVIRIVKGNPV
                     FFSDHMDRLEASARIGNKSLLADRQMLIKDFINLSKTEKKKEINLKIVFNYNKGIENY
                     LLYFIEPVYPSEEQYIKGVKGILFRAERKEPETKVINHKLRSSIFHRLILEGGYEALL
                     VNENGCITEGSRSNIFFLKGETLTTAPDETVLNGITRKYILEICRELKKAVVFRCIPA
                     DSISEFDAVFMTGTSPMVLPFCCIGDVYFQTDIPLIGKLRMSYLEKTEESIRRFISGA
                     "
     gene            17389..17679
                     /locus_tag="PK288_05615"
     CDS             17389..17679
                     /locus_tag="PK288_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18433.1"
                     /translation="MPSVRRLKKDIDYLVFEVISDCFSFGRLHQDEKADEVAGIISDA
                     VCLRNDLIKRVNNPVKGDDPAVIRNHFRNVTKDLYTGIDSLCTRLSGLAPKK"
     gene            complement(17654..18667)
                     /gene="amrS"
                     /locus_tag="PK288_05620"
     CDS             complement(17654..18667)
                     /gene="amrS"
                     /locus_tag="PK288_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPM18434.1"
                     /translation="MGPLYLKYDRYIECRLCPHRCKIPEGKSGICGVRKNTGDRIELI
                     TYNVISGHALDPVEKKPLYHFFPGHKILSIGSYGCNMRCDFCQNWHISQHQQTGFRAN
                     TSIDCLLEEARQAEKNIGIAFTYNEPIIWLEFMRDTAVRFRENGFFTVMVSNGYVNTG
                     PLNEITGFIDAFNIDLKAFNETFYKKLTGADLENVRESLRFIAKKEKHLEITTLIIPG
                     RNDSPEEMRNQVKWIAGELGEDVPLHLSRYFPMYRRQDPATPKSTLDSLHEIASRYLK
                     YVYLGNTDSSSGQDTRCPKCGITVTKRIGYGIRLLNLSNEGNCLGCGTTVYRNFTFSG
                     PNH"
BASE COUNT         5092 a         4339 c         4089 g         5267 t
ORIGIN      
        1 cccctggatc gaatacagga tgagcgccga catggaaagc atcctgaaag gatatcttga
       61 tccgcgtgtc accacctggt tctcgcctgc cctcgagccc gatccaacag atgaccccgt
      121 gggaatatcc ttcccatacg aaggtgtcag gaacggccag acgaagaacg acaggagcgc
      181 tgttgaattt gaccgtattg catccgacca ttccaaaccc tataccattg ttggcgacaa
      241 gggaccgaac tggtttgtca tgagagcttc tgaaacctat ttcctcaggg ctgaaggcgt
      301 tctcgaaggc tggaacatgg gcggcggtac tgtcgaagaa ctttataata ccggtatcgg
      361 attatcgctc gatgaatgcg gattcactga tcaccttaat cttgcaggag aaaactatat
      421 caccagtcca agggtgccga tgaccccggg aattgacaag aatacagttc ttcctgcagt
      481 tgccccgccg ccggcttcaa ccgttcccat tcttttcaat accggcggat ccaaggaaga
      541 gcagcttgag cagataatca cccagaaatg gatcagcctc ttcccaaaca gccaggaagc
      601 ttatgcagag aggagaagaa cccattatcc caagctttac ccaaggcttg aaagcgagaa
      661 tctcgacgtt ccggccacat cacttccggt gcgtctctgc tactggacca atgaatacaa
      721 taccaacgcg aagatggtta atgcagctat ccagatgctg aattcggaaa gcaccacccc
      781 caacggagac aaagcttcta ccaaactctg gtgggataag aaaccataac caggaatttt
      841 tcacgcaagc acaaaattta aaggaaggtg ttctctcaga acaccttctt tttgtatatt
      901 tactgaaaca tttgtgctgt ggaacgaaaa tgtctcgatt gcggcgatat aattcgcggc
      961 cgcagtgata agaaattctg ctcggaccag tgcagaaaca gctataataa ccggctgaac
     1021 cgtgatacga ataattttgt gcgaaacgta cacgggcttc tcaggaaaaa caggagaatt
     1081 ttggctgacc tttataccga aggcagacgc aggacgcata aagacgccct gtttgcactt
     1141 ggttacaatt tcagtttctt cacacatgtg atagaaacct caggctcgga gaaatatctc
     1201 ttctgttttg aatacggttt cagggatgca ggagatgatt tcattgaact gaagtacaat
     1261 aaccagtata ttgattatca ataaaacggg aaatagtact tcagattttc gcttgtaaga
     1321 ggtgtgccat aacctgaatc ctggaaggct tcgcaatatt tcgttttgct gcctttagcc
     1381 tttccgtaca attctatgag tttatccctg aagggttcgg catctgatct cgtgctccag
     1441 gtcctgccaa gccattcacg cctttccctt tctccggcag gaacttctgc aagggcaccc
     1501 ctgttgtagg gaagccattc gtaaacctgt gaagtatgct gatgatacat gtcgatcttt
     1561 ttttcaatca catcatcgat gtcgacgcaa acgtcggcct tgaacggctc cggatgaatg
     1621 aacccgtcgc tcatgaacag gaacatcgga tttttgtcaa ggtgcgggac ttccggaagg
     1681 atcctcggta cagttaccat gtaggcagca tcgagaacca gtgttcccgt gtaccgatga
     1741 tcggggtgat agtcgtaggg ccgcggaaaa ataaccacat ctgctttatg ctcccttatc
     1801 agcctgatta cctgcagcct gttttcataa gtgggcatca gctgaccgtc atgattatcg
     1861 agagtaatgt atttaacgcc tataacctga ccggcctttc tggcttcttc cctcctgatc
     1921 gccgcaagct tgtcgggcgg aattgactga tgtcccgcgt cgccgttggt taatgaaacc
     1981 atcagtacat catgcccgag cctggcccac ttataggcag tgcctccaac tttgtcggga
     2041 tcgtcaggat gtgcccctat aataattatg tgaagctttt tcccgctatc tgtttgctgt
     2101 ccctttactg acaatggaat gaacagtaac aggaagatct gaagaaacaa tgttattcct
     2161 ttcattggat tgaaatttat tttaatgagc ttaggtgatt aatgatgcaa tatataaaaa
     2221 gcggggaaaa tcctgatgat tcccccgctt aaagtttcca ccaaaacaaa tcaactaaaa
     2281 gtagatgcaa aaaatgtcag taacaagata tgtaaattct gtatataaaa tgatgaagaa
     2341 gctgtttctt aactattttt aactgttttt gtgatgcggt gcgcagaccg ttctccggta
     2401 aaagtccatg gaagatctga cgttaaagta cagtttttga gagatgaaag ggcatagtta
     2461 aacaaactga aaaaatgccg gtctgaaact ttttctccag tttttccgtt ttacattcaa
     2521 cttgaatcct taagcaggtc atgcgacagg ttttaagatg tttaactaat acatggaaaa
     2581 atgaaaaaat tatgtttgat tttactggtt atggtgatgt cggcgcctgt ttattcacag
     2641 gtcggtttcg gggttaaagg aggcttgagt acaacaagcc tgaaaatgga tgaccttaaa
     2701 ctaaatactg atactacaat caatgccatt aaaggtgcaa actttggata tcatgcaggc
     2761 attttcctga ggcttggcat gatgggagtt tatgttcaac ccgagctcct ttttacttca
     2821 cgaactaacg aataccggat tgaagacctt gagaaccctg ccactattgt caaacagcag
     2881 ttcaatcagc ttgatctgcc tgtaatgctt ggtttcaagg ctggtccggt caggatcaat
     2941 gccggccctt cagcacggct tctgatcaac tcgccgaaag atctgatcaa tgaccctgat
     3001 tataagacca tgattaaaaa tttcactttc ggctaccagg ccggactggg atttgacata
     3061 attaaaaaga tcactgtgga cctgcgttat gagggcagcc ttcataaata ccagacccag
     3121 atacaaaaat ccaccgggga taaattcaaa ctcgacgaca ggccgaatgc ctttcattta
     3181 agcatcggat ttatattctg atccggcccg ggcccggacc ttatttgacg aaaagcatcc
     3241 tgacagcaac tataagcaga aggacgccaa atattttttt aagtatctgc tgtggcacat
     3301 ttaaggctat tttagagccg aaataggatc caattataaa tgcagccgca aggataaggg
     3361 cgaatttcag attgacatgc cctgctttat aataattata agcagccagg atgcctatcg
     3421 gaggaagcat tactgcaagg ctggtgccct gggcactgtg ctgactgaag cccataagga
     3481 ataccagggc cggtatcatt actatagcac cgccgatgcc gagcataccc gccagggttc
     3541 ccgttaataa accgattgcc aggaggagca gaagtgaagt tgttgtcatc agatcttaat
     3601 aattatgtta ggattgtgat ttgtaaaaat agaataatct ttgaagcaat tgacagttaa
     3661 ttaggtcgtg gctttgggat aattgataat tttgagcccg tcagacgaat gaggcatatg
     3721 cggatttata ttcttgcagc aattttgcct gcagtgatac tgtcaggatt attttgctac
     3781 cttgttttcg gagtaacaat acgcccggcc ggttccggaa gccaggtcat ttacctgcct
     3841 gaagaaagca cattcgaaca ggtgatggat tcattaaaaa caaatctgag aattgataat
     3901 ctgaaagttc ttcaatgggt tgcagaaaag aagaattacc gctctcatat taagcccgga
     3961 cgctatgtca tcgaaaaggg aatgagctgc aatgagctga tcaataacct gaggtcgggg
     4021 aaacagacac cggtactggt tactttcagc aatatcaaaa cgatttacga cctggctgga
     4081 agggttggcg ggaaaattga agctgactca gcccggattg ttaccttttt atccgacact
     4141 gaaaattaca ggtcggatgg atttgtccgg gaaaacatta tttcagtctt tctgcccgat
     4201 acttatgagt ttttctggaa taccggcccc ggtgaattct acagccgaat gctgagggaa
     4261 tacaaaaagt tctggaatgc agaaaggatc agaaaggcag atgaaaaaaa actaaccccg
     4321 cttgaagtgt cggtacttgc ttcaatagtt gatcaggaaa catcaatgcc ggatgaaaaa
     4381 cctgtgattg caggagtata tcttaacagg cttaaacgcg gaataccgct tcaggcggat
     4441 ccgacgatca aattcgtgat aaacgatcct aaaatgtcga gaatacttcg caggcatctt
     4501 tcaatcaatt caccctataa tacatataag tataaaggac ttccgccggg tccgatatca
     4561 tgccctgcca aagacggcat tgaagcggtt ctgaatgcgg ataaccacga ttacctgtat
     4621 tttgttgcca ggtctgattt ctcaggatat catgtttttt ccagaacgct ttccgagcat
     4681 aacagatatg cagccatgta ccggaaagag ctgaacagaa gaaagatctt cagatagttt
     4741 ataatatctc aacctcactt tcaagcatga tcccgaattt ctcaaaaaca gattccttta
     4801 tcttttctga gagatcgaat atttcttttc cggtcgctct gccaaaattc acaaggacga
     4861 gtgcctgcct gtcatgaaca cctgcgtctc cgatacgcct gcctttccat ccgcataatt
     4921 caatcagccg ccctgccggt attttgactt cacccgaatc attattaaaa acaggcagct
     4981 ggggatcctt cttttttaat tcctcagcta atgactcact tactgccgga ttcttgaaaa
     5041 aactgcctgc attgcccaga atcgcgggat cgggaagttt agattttcgg gtgtttatta
     5101 caatttccct gacattcttc agcgaaacag atccgagttt actgacctct tccctcagag
     5161 atccgtattc cagactgagc tttggcctgg tggaaagcct gaagtatact ttggtaatga
     5221 gatatttgcc tttgagctct cccttgaaaa tactgcttct gtatccaaac tgacattcat
     5281 cgtttgtaaa ttccctgaca gatccgtttt ccagacttac tgccctcacc cgctcgatgg
     5341 tattcctgac ttccacacca taggcaccaa tgttttgtac cggggcagct cccacactgc
     5401 cggggatcag cgaaagattt tccagccccc cgaatccgtt atttattgtc gattcaacca
     5461 gtttgtccca caatacacct gctcctgaag atataattac aacatcatct ttccttcctt
     5521 ccagtgtaat accttccatt tccggatgaa tcagggttcc gtgaaaatca ccggtaaata
     5581 atagattgct gccctggccg atcacgaaaa gactctgttc tgatcctccc tgctgacgga
     5641 gaaatgatat aacattctct tcatgtttga aagggataag ccggtccgct tttacattca
     5701 ggccaaaagt attgtatttt ttcagagata tgttttcgta tatcatctcc ggtttgcctc
     5761 cttctcaaaa ttataaaaac atgttatgtt gacaaacagg cataatttac ttttatttga
     5821 aatgccaatt acaataatat aattacaaca gcttacatga ataaatcatc agtgataaaa
     5881 aaaacattcc cattcctcga aaaggggctg acggaagaaa tatccaatgt atctgagatt
     5941 cgtgatcttg ccgccggaat gagtatactg ggagaaggtg attacattaa atcatttccg
     6001 atggtgcttg caggctgcct cagcgttgta aggctttccg acgacggcaa tgaacttctc
     6061 ctctattacc tgaaccaggg agaaatatgt gcaatggccc tgacttgctg tatgggcccg
     6121 cagaaaagca atatccgaat gaccgccgag gaagattcgg ttattctgac cgtacctgtt
     6181 gaatatattg agaaatggat gtcagattac agatcatgga aggaatttat gatgtattcc
     6241 tacaggaaga gatttgatga gcttattgag ataattgatg cgattgcgtt catgaaactt
     6301 gatgaaagac tgatccggtt tttccgggaa cgattcaggt cgacgggaaa aaccgtcttc
     6361 accggaaccc accaggatat tgccctgcag cttaatacct caagggaagt gatcacgcgg
     6421 cttctcagga accttgaaag aaacaatctg gtaataactg aacgtcattt tattgattat
     6481 tccctccttg tgaaaaaaga tacttctttg tgacttaagt cactgacggg taacttgcag
     6541 acacccaaat ttgcaaaaaa ttccggaaat gacagatttc attagaaaaa atctatttgt
     6601 tataatattc acagcagcgg gcgccgcagg aggatttctt tactggagat atgtcggatg
     6661 cctttcgggc acctgcatga taaaatcggt gtggtatatg agtactcttt acggcatggc
     6721 ggtgggctgg attggcggaa gcctgctcaa tgatcttttt ctgaaattta aaaaagcagg
     6781 gaagaatgaa tgagagtttc caggcaataa ttgaaggaag aaccccggtt ctggttgatt
     6841 tttcagctga atggtgcggc ccgtgcaaaa tgatgaagcc tgttctggaa cagctaaagc
     6901 aaaaaatgaa agaccggata cgaataataa agatcgacgt tgacagaaac agggaacttg
     6961 ccatgaagta taggatcagc aatgttccga cactcatgct gttttctgaa aacagaattc
     7021 tctggtccgg ttcgggagta gttacagcaa atttccttga aaatgttatt aacaataatt
     7081 gtcctgtccc tgctgatcag aaggttaatt aatctgcgca gagttactga taggacttca
     7141 gctccgctct ggcatttccc ccggtttttg cggatcaggc gggttcttgc tgccggtttc
     7201 tgcgattgaa cggcaatctt caggactgct ggtttatcag cagtcatgga aatttcccct
     7261 taattgccgg attgcttttc cctttccgat aattgctttt ctgcctggtt gactttatct
     7321 ggagtctgta ccggattatg catgaaaaga ataaatttcc tgcctgcatt gaaaaaaaat
     7381 gtaaattcgg gaaataaatc cattatttaa actgaataca gttatatcat gaaaagcaat
     7441 cgccgtgatt tcctgagaaa ttcaacactt gccggagcag gtgcttttgt cggcagtttt
     7501 ctccagggtt gtgtacccgc gaaagaagaa tcgaatctgg ccgggatcat tgaagcagta
     7561 aagaagcccc atactcagaa attcaatatg tcgggatatg ctgccccgcc gctgccggta
     7621 gtaagggtag ggtttatcgg tctgggcgac aggggcagtg gtgcagtaca aaggctttca
     7681 tacatagaag gcgttgagat cagggcatta tgcgacaaaa gagaggcagc tgtgaaagca
     7741 tcgcagaaat atcttcagag tgtgggacgc cccgtcgctg aggaattttt cggaacggag
     7801 gacagctgga aaaagcttgc agaaatgccc gacattgatc tgatctatca gtgcaccccc
     7861 tgggtgatgc atacaccagt cgcagtatac gccatggaaa acggaaagca tgtggcatgc
     7921 gaagtcccga ttgcacgcac cattgatgaa tgctggcagc tcgttgaaac ttcagagaag
     7981 accaggaagc attgcatgat gctcgagaac tgctgctacg acttttttga gttgcttacc
     8041 ttaaacatgg ccagacaggg aatgtttggc gatattgttc acggtgaagg cgcctacatt
     8101 cacaacctgg ttgatatgat cttcagaaag cccgacgatt caagacagtc ggataatgcc
     8161 tacaccgaca tgtggaggct taaagaaaat gctgaaagga ccggcaatct ttatcccacc
     8221 cacggactgg gacctgtatg tcaggttatg aatattaacc ggggtgacaa gatggattat
     8281 cttacatcaa tgtccacaaa cgatttcacc cttggaaagt atgcatccgg gcttgcagcc
     8341 gccgatgatt tctacaaacc attcgttggc aggccgtaca ggggaaatat gaatacttca
     8401 cttgtaaaaa caagccttgg caggacaatc atgattcagc acgacatatc atctgtgcgc
     8461 ccatattccc gcctccacgt gatcagcggc acctccgggg ccgccataaa atatcccgaa
     8521 ccggaaagga ttgccctggg ccatgaatgg atcagtgaaa aggagatgga agatgtaatc
     8581 gacaagttta cacccgaaat tgttaaaagg gtgggagagc tggcaaaaaa ggttggtggc
     8641 catggcggaa tggactttat tatggactgg aggctcattg actgcctgag gaacgggttg
     8701 ccgctcgatc aggatgttta tgacggtgct ctctggagtg caatagctcc cctgagtgaa
     8761 tggtccgtcg caaaccgctc acaatcaatt gatgttcctg actttacagg aggatcatgg
     8821 aagactaaca aaccccacga cataaatctt gaaaaaggcg gtaacaccct ggtactggaa
     8881 ataaaagaat caaaaccgga attgcagctt aacataagct aattttggga taatcattgc
     8941 gggcagtgcc gagccggata tgggcaatcg tcattagtat cctgcagcct gcccgtcttt
     9001 tctcgattcc gaggcaccat accatacttt gttgcgttca tccagcatta tggcctggta
     9061 acctccgtaa ccgcccagat cccactggat ggtatggccc attttaagca gcttctgaat
     9121 tacttcccac ctaaagccgt tttcaagaag caatattccc ccgtcggtca tctgctcccc
     9181 ggtgggctcg gaagatccaa tatgatggat cctgggagca tccccggcct cctggagatt
     9241 cattttgaaa tcaatcaggt tgacaactat ctgggcatga ccctgcggct gcatgtctcc
     9301 gcccatcact ccgaaactta cccacggcct gcccttcctg gtgataaagg caggtataat
     9361 ggtgtgaaat ggccgcttgc cgggtgcata cacgtttgcg tggccttcgg aaaggctgaa
     9421 catttcgccc ctgtcctgca acacaaaacc cagaccgggc ggacacattc ccgaacccat
     9481 gcccctgtaa ttactttgta taagggaaac catgttgcct gatctgtcgg caacagtcag
     9541 gtaaaccgta tttcctgctt ccggttttcc cggatcgaaa atctttccag ctttcccggg
     9601 attaataagc ttcctcctct cagcggcata ttttttcgat atgagccgcg acaccggaac
     9661 tgcactgaac aacggatcgg cataatatct tgccctgtct tcgaacgcca gcttcttggc
     9721 ttcggtgaaa acatgaatat attcacttga gccaaatccc atggaagcaa tgtcaaatcc
     9781 ttcaaggata ttgagtatct ggagggctgc aattccctga ccattgggcg gaagttccca
     9841 gacttcatac cccctgtaag aagtacttac cggctcaacc cattcggaat gatgcctgct
     9901 caggtcatca tagctgagga atcctccctg cgacttcatg aaagcatcta tggtttttgc
     9961 aatgctgcct ctgtaaaact catttcttcc tccctttgca atcttttcaa gagtgcttgc
    10021 caggaaggga tttctgaaaa tctccccttt ggccggagac ttgccacccg gcatataagt
    10081 ctccttgatg ttcggatagt ctttaaggag ccgggtgtta ctgtcgagat aatatgcaat
    10141 gagctcgctt actgggaacc cattgcgggc gtagttgatc gcaggttgta agatatcctt
    10201 catcggaagc cggccaaaca tttcatgcat ttcaaaccac ccgtcgacac atccggggac
    10261 cgagaccggc agcggaccat aggaagggat tttctcaata ttattgttgc gaaaatattc
    10321 cagcctgagt ttcctgggtg attttccgct ggcattcagt ccatacaaac ggcctttctc
    10381 cgcactccag ataatcgcaa aaagatctcc tccaataccg catcctgttg gctcaacaac
    10441 acccagaacg gcatttgccg ctatggcagc atctatggca tttccgcctc ttttaaggat
    10501 gtccagggcc acctgggttg ccaggggctg actggtggca gccatgccat tctgggcaat
    10561 gacttcggat cgtgttgcaa aatcccttcc cgtgatcctg tcctgggagt aaagggaaat
    10621 gttggtcaaa ataaggcctg caacaataat ctgcaatttc attgtagtct gtttgtgttt
    10681 acttttcaga ataccggtct gtttttttct gccggctgtc tgccttgttt gagagaatat
    10741 cgatcactga ttttgtcatt gctgcaaccc ctgctgtgaa tgcaggctca aaatcaggag
    10801 caaaagacga attgtgaagt gaaggaagag gattcgcact ggcaatatgc tcatcatact
    10861 tctgcctgtt tacccctccc agccagaaaa gtgcaatggg cactttttct gctgtctgtc
    10921 catatttccc gaaatcctct gcaaccgttg ccggttcaac ctctgtaagg ttttttccac
    10981 cgagaatatc actcattgac cggacgcagt tcatcaccag ttgagggtca tttgacacag
    11041 gcagatcggc ttcactgctg aaagtaacga gaggcatttt atcttccggg agacctgctg
    11101 ctatccccgc gccgcgggta atattaatca gcgattccct gatggtattc aatacgctct
    11161 cttcaaaata tcttatcgtc agcagcattt ttacttcatc gggaattaca ttggctctgg
    11221 ttcctccatt tattgctccg acagtaatga cggcaggttt taccggattt atctcccggc
    11281 tcacaattgt ttggatatca agaatgatac gggctgcaat aacaacaggg tcaatagtgt
    11341 tgtggggcat ggctccatgc cctccaatac cgtaaacagt aatctgagcc gagctgacgc
    11401 ctgcaaaaat cggacccggg aaataaccta tactgccggc aggcaaatct gcacttacat
    11461 gatagcacag tgcacagtcg ggtacccgga gcctggaata gagattatcc ctaagcattg
    11521 cctcagcacc cctcatacct tcttctgcag gctgggccac agccaataat gtaccgctcc
    11581 aatgatcttt catcctgatc agggtttgca gggtgccgag ccagaccgac atgtgcagat
    11641 catgcccgca ggcatgcatt acaggggttt ccctgccatt catatctttc attacaacgg
    11701 tactggcata aggaagccct gtcgtttctt ttaccggcaa agcatccata tccgtacgca
    11761 acatcaccgt ctttccattg ccgttaacga aaatcccggc aactccgtga ccgccaatcc
    11821 cgcgtgtagt ctcaaaacca agcttttcaa gattgtctgc cattattgca gaggtccttt
    11881 tctccatcat tgaaagctca gggttcatgt gaagatcagt gtagatcttg tgataatgtc
    11941 cgattatctc gcttactgat ttgaaaatca actcattcat attatttttc tggcaaaaga
    12001 tgttagttga gccaagtatt atcaggaata ttgaaagatt cgccttcata gtatcccgga
    12061 ttctccagat tattcccttg attattccat cgctttaatc ctcagttccc gaggcatttt
    12121 ttcaattgca tacctcaggg aggtgcgggg catgattccc ttattcctgt aaacataatc
    12181 aaatacttcc atcttatgaa gatgactcgc agattttaac atccagccat atcctttctg
    12241 caccatatca tccttatcca aaaggaggat atcggcaatc tcaaatatct cttcaaggta
    12301 caatcccttc ctggcaggaa tgatcaggga cacggctgaa gctcgccgca tccagcggtt
    12361 agctgatcct gtccactttt tgagtatcct gattgattgc ggaaacattt ccacaagcgc
    12421 tccaacagtg tggttgcaaa aagtatcgca cgaggcccag ttggatatat atctgtcaac
    12481 ccagtagccg aatatttcaa tatcagatgg ttcaaactct gttctgacgt tatatgacca
    12541 gttgcacgcg ataaatgatt cctccagcat tcccgatttc cagagctctt cacaatgact
    12601 gaatatatta tccttgcttt tattggtcag atttttgaaa atcctttttg ctatgcctgt
    12661 cacttcagcc gactttactc cgtataatct gacttcttcc ctgaaaaatc ccctggctga
    12721 gagcctgtgc gcttcctctg aatgtcggac cagttcctgc ctgatctctt caataatagt
    12781 gttcatattt cttattcagt taaaacctgt cccggtacag ccacacgatc ctgtcctgaa
    12841 taatgaagct gccaccggta atctgcacac gtgagtttgc cgttactttc caatgtgatt
    12901 catatttatc cctaacttat tcaatctgat ggttttgtct gtcccggctc caatcctatt
    12961 tgaggtactt ccagaatttc tcttcagatc tctgaaactc gattttcttt tttctctcaa
    13021 gtatataata cagcagcagt gaaattaccc ctacgatcaa aacaccgatg atgaaatatc
    13081 cgagtatccc cgatgcactt gcatcactga tatttgctga accttcagta attcctgaaa
    13141 tcgtaccagt actattgcgg cttccaacgt caacactgaa aacattttta acaaaataca
    13201 aggtcaggca ggcaagcaag ccggaaaata cgggtgttat cagccagcct gcacctatac
    13261 tcccaagcag tctgaaattt atgtttcttg ctcctttgta aaatccgatg ccgattatac
    13321 ttccaattac cacctgtgtg cttgaaaccg ggacaagggg gagggggaaa agcccgttat
    13381 ttttcaggaa ttcggaaatc cccgaagaag aaaacaggaa taaaacaatc ccctgtgaaa
    13441 gaacaacaac gagggcagct tcagacgaca gttccacaat gctgcttccc actacctgca
    13501 tcactttgcc ggaataggag agaatgccgg cggcaattgc cagcccgccg agaagaaaca
    13561 gttgttgggg ggaagacagg gtgaaaaatc caaagctgag ttcctgcagg ttaaatgcag
    13621 gaacgaatac acccatgaca tttgctatat tgttagcgcc caggctgtat gctccgaatg
    13681 ctccagcaat gataaggctg gtccttatgt aggactcaaa ccggatcagg tggatcccgg
    13741 cctttttctt tatcttcatc aggatactga ccagtatcac tgcaaaaatg gcgcccagaa
    13801 caggtccggc tacccaggtc gttacaatct gtgtaagaac tgatctgtcg gttgtattgc
    13861 ctgtaaagag gttccagccg atgattgctc ctacaataga ctgggtggaa gatacgggga
    13921 tggaaaattt caccatcaga tatactgtga ctgctgcggc aagagccacc gtgaacgatc
    13981 ctccaagtgc attcactgcc ccaagtttgc tgagggtttc cgatgtcccg gacccctgaa
    14041 acactgcacc cagaataacg aatacggagg caatgatcgc tgccttccag aaactgatca
    14101 tttttgatcc aactgctgat ccaaagacat tggcggcatc attggcccca agtgaccatc
    14161 caaggaaaag tccgcttgtc agaaacagta ttatcatgct ccggttgtaa ttctcagatt
    14221 tatggtttcc ttcatgcaat taacgctgaa cgtttccatc tttgcctgcc gtatatattt
    14281 aatttaacag aacccaatgg tcccgaaagc tgaatccggc ttcgaatcct tttaacacaa
    14341 ttacatcaat attttaattg acatcgcaga caacagatct gcagctttct gggcattgtc
    14401 cgaaatatcc ccgatgcggt tgataatata tctcagatgg ttcttatgcg ccaattcaag
    14461 ttctttcatt tcctggaaga taattttctt agtgcttttt gttattttgt ccgtctcgct
    14521 ttcaaagtaa taaactttta acagcttctc ccgaaccgta taaggtgcct tgaaatatgc
    14581 tcttgcagcc ggaaccagtt cctcccctgc tgataccgag gcttcggtta tgttgagaaa
    14641 atcgcggttc agtgacgggg gaatatctgg taattcaatg ctgaactcat caagtgacga
    14701 ctttatggta tcaagtattt catccattac gtctatgagc gttattacct cgcccctgtg
    14761 ctgcggcaga attgaatggg ttatcatctc attctcaatt gaacgttgca gcctgtcggc
    14821 agtgctttca agggatccga ccttttgaac atgcctttca aatgcttcca tgtccttttg
    14881 aagataatcc tttactgctt ccctgaatac cagcaggccg aggtcgacat tatcgaaaaa
    14941 ctcatctatt ttgagaatga ggttttttgt agttctctta aaaacaggca ttatgttgaa
    15001 aatattaaaa tgttaaccaa acgatggtca gaactcgata atcctatcaa ctttataacc
    15061 tctcagatat tgtttcagaa tgagctgaat gtcgcggtta tcctggcaat ccatgatgca
    15121 gtcatgcagg gcggtcagtc cgaatcccat ttcgtttata ttgaaatatc cgtaaccggt
    15181 aaaacgacca ttaacgatct taactgcact gcgttcatct gaacttctgc ccctgtcgat
    15241 tatgaagtaa ttcttccggc tgaatacaaa ctcctcggaa gccttttttg cacgttcgtt
    15301 ataactggct gccggttctt ttccacagca ggcaccccgg catacgccta catggtagtg
    15361 gaagcacggg ccggatgtat tgtacagccc tgtgagtttc tggcacaatc cgtacttcac
    15421 gaccagttcg gcaagtctgg atcttgccct ttcctttgag gcaaaaatcg caacaggaat
    15481 gtcgtttccg gctattctgc ctgctgaaaa attcatgtat tccttatcat ccctgaaaca
    15541 gaaaatcccc cagcgctgtt ccgtgttgcg ctgggctttg ttgtaaaccg gtttattgta
    15601 tttgatctca tatgattcct ttataagggc aataagctca cttcctgtta tttcccagct
    15661 gaaatcagct attttatccc gtattgccat gctccgctct gaagtgttgt ttgaaaggtg
    15721 tgtcgctatc ctttgtttta tgttgcggct tttaccaata taaataaggt cgccgcgctc
    15781 attgtaaaaa taatatatcc ctgtttcttc aggggtttta tcaagttttg aagtgtcgag
    15841 gctggaattc agctttgaga ttttcgtgtt gctgatgagc cgggacgact ttccggtcag
    15901 ttccctgtca atctcgataa gtctttcaaa cagccttgca gttgccatgg catccccggc
    15961 tgcacgatgt cttccgttga tctcaatgcc aatgtgccgg catatgctgc cgagattgta
    16021 tgattcatac cccggtatca gcttgcgact caggctgaca gtgtccagta cctgtctcct
    16081 gaaattatat cccaatgaac tgaattcctg ccttaaaaac gaatagtcaa actttgcatt
    16141 atgagcaacg atgatccggc caccggtaaa ttcaattatc ttttttgcta tctcaaaaaa
    16201 cctcggagcc tcatttacca tctcatttgt aatcccggtc agactggtaa tataataagg
    16261 gatattcctc tcagggttaa ccagcgagat gaattcatca gtgatcttcg atccgtcgtg
    16321 ctgataaatg gcaatttccg ttattctttc attctttgca ctgcctcctg ttgtttctat
    16381 gtcgacaatt gcatacatat ttcgtatcga ttcagctgtg aaactgctaa gatatggaga
    16441 aatagcggca caatatctcc aatatattaa ttttgtatta ctataattat cagttatgaa
    16501 cgaatgtttc ggcagaaaat ttgtcctgaa tggttcactc atgcctgcag aattgtttga
    16561 taattcaatg gtgtatgaag gcgactcagt ttatgaggtg atcaggatcg taaagggtaa
    16621 tccggtcttc ttcagtgatc atatggatcg tctcgaggca agcgccagaa tcgggaataa
    16681 gagcctgctg gcagacaggc agatgctcat aaaggatttc ataaatcttt ccaaaactga
    16741 aaaaaagaaa gagatcaacc tgaaaatagt ttttaattat aataaaggga tcgaaaacta
    16801 tcttctctat ttcatagaac cagtatatcc ttcggaagaa caatatatta aaggtgttaa
    16861 aggtatattg ttccgtgcgg aaagaaaaga accggaaacc aaggtcatta atcataagct
    16921 caggtcgtca atttttcacc ggcttatact ggaaggtgga tatgaagcct tgctggtgaa
    16981 tgaaaatggc tgcataacgg aaggcagcag atcgaatatt tttttcctga aaggagaaac
    17041 cctgacaacg gctcctgacg aaacggtatt gaacgggatt accaggaaat atatcctgga
    17101 gatctgcagg gagctcaaga aagctgttgt gttccggtgc attcctgcag attccatcag
    17161 cgaatttgat gcagtgttta tgaccggaac ttcacctatg gtcctgcctt tctgttgtat
    17221 tggggatgta tacttccaaa ccgatattcc tctcatcgga aaacttagga tgtcatatct
    17281 tgaaaaaact gaggaaagca tacgcagatt tatatcgggc gcctgacgac aggcagtgat
    17341 ctccaattct ttttttatct ttgaccttta attattaaaa acaactgtat gcccagtgta
    17401 agaagactta aaaaggatat tgactatctt gtttttgagg ttatctccga ttgcttttcg
    17461 tttggccgtc ttcatcagga tgaaaaggct gatgaggttg ccgggataat ttcggatgcc
    17521 gtttgcctga gaaacgacct gataaaacgg gtaaacaatc ctgtaaaagg tgatgatcct
    17581 gcagttatca ggaaccattt cagaaatgtg acaaaagatc tttacaccgg tatcgacagt
    17641 ctttgtacgc ggctcagtgg tttggccccg aaaaagtgaa attccggtat actgtggtgc
    17701 cgcacccaag gcaattacct tcattgctga ggttcagcaa cctgataccg tatccgattc
    17761 ttttggttac cgttattccg catttcggac acctggtatc ctgcccggat gatgaatcgg
    17821 tgttgcccag gtaaacatat ttgagatatc tcgatgcaat ttcatgcagg ctgtcaagcg
    17881 tggattttgg agttgccgga tcctgcctcc tgtacatcgg aaaatacctg ctcaggtgca
    17941 acgggacatc ctctcccagc tcaccggcga tccattttac ctggtttctc atttcctccg
    18001 gactgtcgtt ccttcccgga atgatcaggg ttgtaatttc caggtgcttt tctttttttg
    18061 caatgaagcg aagactctct ctgacatttt ccagatcggc cccggtaagc ttcttataaa
    18121 atgtttcatt aaacgccttc agatcgatat tgaatgcatc gatgaagccg gttatttcat
    18181 tcagcggccc ggtattaacg tatccgttgc taaccattac ggtaaaaaat ccgttttccc
    18241 tgaatctgac ggctgtgtcg cgcataaatt ctaaccatat aattggttca ttgtaggtga
    18301 aggctatccc tatattcttc tcagcctgcc tggcttcctc aagaagacag tctatgcttg
    18361 tatttgccct gaaacctgtc tgttgatgct gggaaatatg ccagttctgg caaaaatcgc
    18421 atctcatgtt gcacccatat gaaccgatgg acaggatttt gtgtccgggg aaaaaatgat
    18481 atagcggttt tttctctacg ggatccagtg cgtggcccga tatgacatta tatgtaataa
    18541 gctctatcct gtcccctgta tttttcctga ctccgcaaat gccgcttttc ccctcaggga
    18601 tcttgcatct gtgaggacac agcctgcatt caatatacct gtcatattta agataaagcg
    18661 gtcccatttt tctggattca ggaataaaaa tacattaatt cgtataggaa tgaaaattaa
    18721 gctactttta tatacaatag ttcgttaaag ttttaagcgg ccatagtgcc gtataatata
    18781 agttctc
//
LOCUS       DAOOYG010000044        18456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2896_length_18456_cov_6.187544, whole genome shotgun sequence.
ACCESSION   DAOOYG010000044 DAOOYG010000000
VERSION     DAOOYG010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18456
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2896_length_18456_cov_6.187544"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1319
                     /locus_tag="PK288_05625"
     CDS             108..1319
                     /locus_tag="PK288_05625"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPM18435.1"
                     /translation="MIILVLNCGSSSIKYQLFDMSNGCNMLAKGLLERIGLSNSVLTH
                     KPTGKEPYTVTNDIPDHTTGINMVMEALTDPGHGVIADIGQIKAVGHRVVNGGEIYKE
                     SILVDNEVKKQIELNSELAPLHNPANLKGILSVEQLIPGVPQVAVFDTSFHQTMPDYA
                     YMYALPYEYYEKYKIRKYGYHGTSHKFVASKAARLMGKDVRDLKIITCHLGNGASITA
                     IQWGRSIDTSMGFTPVDGLIMGTRTGEIDPGVLIYIADKEHLNVTGINNIINKKSGVA
                     GISQLSSDMRDLEIAAAEANEKAILALNMYAYRVKKYIGSYIAALNGLDLLVFTGGIG
                     ENDHKMRAMICSDMENLGILFDFKANDGVRGKDFVISRPESKVKVMCITTDEEFVIAS
                     DTRSIVEHHSV"
     gene            1447..2349
                     /locus_tag="PK288_05630"
     CDS             1447..2349
                     /locus_tag="PK288_05630"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPM18436.1"
                     /translation="MVLKSLSDLKGIVSGKPRKKLVLAAAHDQHSLGAVIQAWHDDIV
                     EPILIGDARAIQNICMEYGYNIDGLQVIHEPVTEMSVERAVRMLNGKQADVLMKGKVG
                     TTILLKCVLNREWGLRTGNLLSHIALFEVETYHKLIAVTDVAMNIAPNLKDKIAIVNN
                     SVSFLHKLGYVMPKVAVLGAVEMVNENMEATLDAALLSKMNQRDQIKNCIIDGPLAFD
                     NAISYESAKHKGIRSEVAGDTDLLLMPDIEVGNVLYKSLVFFARAKVASIILGAQVPI
                     VLTSRSDSEQAKYDSILLSAAASN"
     gene            2356..3258
                     /locus_tag="PK288_05635"
     CDS             2356..3258
                     /locus_tag="PK288_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPM18437.1"
                     /translation="MIRSLDQMVAKVLSGGRKYRIAVAWAHDDNTILAIRKAVEEGIA
                     EALLTGRKKEILKNCQAWGIKPENFTIIESDDEVSASADAVKLAKTGDADIVMKGLAG
                     TSHFLKAVMDKEKGLMLPGAVLSYVGALQLPAYNKLLFITDPAVIPFPGLEQKIAMAE
                     YAIEMTSRFGIEKPKIALIGASEKPSRHFESSAHYEAMKKMAAAGQISTCIMDGPLDI
                     FLACDRKSVEIKGVKTPVDGDADILLFPSLESSNPFYKGLMLFAGGELGGLIRGTEKP
                     VVLMSRSESEKSKFYCIALACFMA"
     gene            3301..4377
                     /gene="buk"
                     /locus_tag="PK288_05640"
     CDS             3301..4377
                     /gene="buk"
                     /locus_tag="PK288_05640"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPM18438.1"
                     /translation="MEKILILAVNPGSTSTKFAVFEENRLLFEKTLRHTPMELEPYRK
                     VTDQLRFRKDMIVKELTARNVDFTRIRAIVGRGGLVKPIESGIYEVNDRMRRDLETGI
                     MGEHASNLGGLIAADIASSLPGAKAYIVDPVVVDELQPVARISGHPAIERISIFHALN
                     QKAVGRLYASSAGRRYEDMNLIIAHMGGGISVGAHRKGRVIDVNNALNGDGSFSPERS
                     GGLPSGQLVDLCFSGKYSHQEIKSMLTGKGGMMAYLGTNNFIEMCDRADQGDKEAILI
                     RDALAYQVSRDIGAMAAVLEGQVDAIILTGGLAYHEEHVQRIKKMVGFIAGIYVFPGE
                     DEIKALAFNGLLALEGKIEIKTYV"
     gene            complement(4374..5069)
                     /locus_tag="PK288_05645"
     CDS             complement(4374..5069)
                     /locus_tag="PK288_05645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM18439.1"
                     /translation="MNILISGGSKGIGREIVRIMAADKDNQILATGRDEPALKELADS
                     LPHGNVSYMVMDLADMASRLPSIKKQILSRFSRIDILINCAGSLTVKDFMDITDSEAR
                     MMMEINFFGPAALIKLVVPLMGEGSHIVNISSMGGFQGSSKYRGLSYYSASKASIACL
                     TECLASELSGSGISVNCLALGAVQTEMFGTAFPGMNAPVMPEQMAGFIAYFAVSGNKF
                     FNGKILPVAIADP"
     gene            5322..5888
                     /locus_tag="PK288_05650"
     CDS             5322..5888
                     /locus_tag="PK288_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18440.1"
                     /translation="MYAIRKTGLIFFLVILSAVAVSGQEKSKDKPPLSERLFFGGNIG
                     LAFGTITDIELTPLAGIWVLPRLNIAAGPTFRYYKHPYYGKTMIYGGRTYMEFMFLQD
                     IDNLVPLGIHSGLFIHGEYELLSLETAFFGESFNTRRFPTGTFLAGGGISQYLSQRSS
                     LNISFLWALNDAGYGLYSNPEIRISFIF"
     gene            complement(5988..7223)
                     /locus_tag="PK288_05655"
     CDS             complement(5988..7223)
                     /locus_tag="PK288_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPM18441.1"
                     /translation="MSKPRLSLPLHYQILIMLLAGGFFGYYFTPAATYTNWMGDLFLR
                     ALNMIIVPLILLSITTGVASVGSSGNLGRLGLKTMGYYVFSTLAAILTGFILVTAIKP
                     GEGADLGFKASVEGIAGASDSFGQTLVKIVPMNIFEALAQGQMLSIIFFSILFGFLMT
                     KIGEKQRDTLMDVFNAALAVIMKITLFIIRFAPLGIFSITAKVIAQQVEMGNNIGEVV
                     GRLGLYVITVLAGLLFHGLITLPVSLKLFGKANPVKHFRNMATPLLTAFSTSSSNATL
                     PLTMEAVENKDGVSNKIASFTLPLGATVNMNGTALYECVAVLFIAQAYGIDLSFGKQL
                     IVIFTALLAAIGSAGIPMAGLVMMVVVLRAVGLPLEGIGLILAVDRILDMFRTTINVF
                     GDTCAAVIVAKSEGEQLNV"
     gene            7839..8285
                     /locus_tag="PK288_05660"
     CDS             7839..8285
                     /locus_tag="PK288_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPM18442.1"
                     /translation="MLLRERIQDFLKKENKTSAQLAEEIGVQPSGISHILSGRNNPSL
                     DFVIRMLEKYPFLSTEWLLFGRGSMYKGHFAGSLFDEPPGDDDKTGLAPAGAAKQAAD
                     DIRLYDEQDDKAESEGDRQKPPGKRLRRIVWFYGDNSFEEFFPGGD"
     gene            8346..9113
                     /locus_tag="PK288_05665"
     CDS             8346..9113
                     /locus_tag="PK288_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPM18443.1"
                     /translation="MPKRIEDFVIRGNKRLNDDFFVLELAAGTPLPDINPGQFAEVKV
                     EGSPETFLRRPFSIHDVDYRENTIKLLIQIAGKGTETLSGLRPGNSLNLVYPLGNSFT
                     IPSAGEKVLLVGGGCGIAPMLYLARKIAQAGIVPGILLGFRNKKRVIEYEDYQEIGDV
                     YLTTEDGSDGVKGLVTGHPVLMNSVFDRMYCCGPEPMMKAVARHCGNAGIACEVSLEN
                     LMACGIGTCLCCVVDTVRGNLCTCTDGPVFNTKDLKW"
     gene            9107..10021
                     /locus_tag="PK288_05670"
     CDS             9107..10021
                     /locus_tag="PK288_05670"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPM18444.1"
                     /translation="MVNLGISIGNLKFKNPVLTASGTFGNGTEFEDFFDVSRLGGIIL
                     KGTTIEPREGNPYPRMAETPSGMLNTVGLQNKGMDYFERNIYPKVSLYKTNVIANING
                     SYIEDYVALAERFDKLGKIAAIELNISCPNVKMGGMVFGTDPASSRKVIRAVREAYSG
                     TLIVKLSPNVTRIEDFARIAEEEGADAISLINTLLGMSIDIESMKPSLSTITGGLSGP
                     AVKPVALRMVWQCARAVKVPIIGIGGIMNASDAIEFFLAGASAVQVGTASFIDPVSSV
                     RILEGIEKYLEEKKIGDIKEIIGLINSI"
     gene            10184..10657
                     /locus_tag="PK288_05675"
     CDS             10184..10657
                     /locus_tag="PK288_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HPM18445.1"
                     /translation="MTGNIQIDNLDRKILDIITKNARIPYLEVARECNVTGAAIHQRV
                     QRLIKSGVIKGSEFKVDPVLVGFKTCAFIGVYLDHPGHYRDIIKKFREISEIIECHYT
                     TGNYSLFIKVYTHDNEHLRDILTEKIQVIPGVIRTETLISLEESINRQIPVLSEK"
     assembly_gap    10801..10900
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11059..11134)
                     /locus_tag="PK288_05680"
     tRNA            complement(11059..11134)
                     /locus_tag="PK288_05680"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11099..11101),aa:Lys,seq:ttt)
     gene            11282..12103
                     /locus_tag="PK288_05685"
     CDS             11282..12103
                     /locus_tag="PK288_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM18446.1"
                     /translation="MGNSIFRDKVVIITGASSGIGEAASREFARKGSKVVMAARSLAK
                     MAAIADEIKAGGGEASYIETDVSKEADCRRMVEFAVSRYGTVHYLINNAGISMRALFD
                     DVDLKVLHRLMDVNFWGTVYCTRYALPYLIKNRGSLVGVSSVAGFHGLPGRTGYSASK
                     FAVHGFLETIRIENLKKGLHVMIIAPGFTATEIRKNALLANGSAQGESPRKEENLKSP
                     DYVAKWILKGIRKKKRNKLLTWDGRLTALFQRIIPALVDLGYYYEMSREPDSPLK"
     gene            12213..14399
                     /locus_tag="PK288_05690"
     CDS             12213..14399
                     /locus_tag="PK288_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPM18447.1"
                     /translation="MPETQRINRAYRALLRTAGNTLTQDDVRKVRKALDYALKASSGN
                     TMLTGQPEVIHALSVATIISGEMGLGLTSIITALLHDSYGRLEADRAVLEKEFGKKVV
                     EILQGFARVSGITSMQSSYQAENFRKLLLSLADDVRVILIKLVERLEYMRNLDKVPEK
                     EKLPLASETYFLYAPLAHRLGYYNIKTELEDLAVKYLEPDQYNYVEGRLKQTTASRNR
                     LIREFSAPLTEKLDGLGIKYTIKSRTKSIHSIMLKMKNQGVDFDEVFDLFAVRIMVDS
                     DPENEKSDCWRVYSVVTDLYQPNPSRMRDWISVPKSNGYESLHITVVGPRGKWVEVQI
                     RSRRMDEIAEKGLAAHFKYKGIKGEGGLDTWLTRMRQILESSDREDRAFLDQVRSGLY
                     TDEVFVFTPKGDLRQLPAGATILDFAFDIHTAVGASCVGGKVNGKNVTIRHVLQNGDH
                     VSVLRSKSQKPKQDWLSFVVTSKAKTKIKQALSEERTKAAAEGKEMLMRRLKNWKITY
                     GDAVIQRLIIHYNLKTAQDLYCNIQSGKTDLLHIKEILLKPGDDSEHIHTHEAAAEKE
                     KKEPAGSPYSDYLIIEDSIEGLDYKLAKCCNPVFGDSIFGFVTISEGIKIHRTGCPNA
                     HNLISRYPYRVVIARWTKAKGAPSFHAVIKITGVEDIGIGNKIADILSDYSVTVRNFS
                     YGMNEGIFEGTLSIMVPNNDVLYGIIRRISSVRGVIKAVRHTSEQN"
     gene            14420..15112
                     /locus_tag="PK288_05695"
     CDS             14420..15112
                     /locus_tag="PK288_05695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPM18448.1"
                     /translation="MLFRNLIFVSLAPVLIIAFYIYHSDRYEKEPLSVLLKALFTGAL
                     TIIPAVIIEGLIAEPSGAGEGLASAGYTAFVVAGFTEELMKYLAFYFFFWQERNFNEK
                     FDGIIYAVYISLGFAAVENILYVFSGGMGVGFVRAFTAVPAHALFGISMGYYFGLARF
                     DSRYRILKLMLAFIVPFFFHGLYDFLLMSKSQVLLTVFVPVFIYYWATGFRKISKLSD
                     ASPFRDSPGRRS"
     gene            complement(15192..18128)
                     /gene="dnaE"
                     /locus_tag="PK288_05700"
     CDS             complement(15192..18128)
                     /gene="dnaE"
                     /locus_tag="PK288_05700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPM18449.1"
                     /translation="MYLNCHSYYSLRYGMMSVEQLVQCAIAANAPAIALTDINNTTAI
                     PMFAGECARNGIRPVAGIEFRDGNELRYIGIARNNKGLRELNEFLSKINSEKAQVPFP
                     APVFSESYVIYPLNKRPGRKLSENERIGIKPRELGSLLTSARPSDRNSMVLLQPVTFS
                     SENEIPIHRSLRAIDNNMLLSHLNPSQCAGEDEFFMDPSRLKQLLEENRWIAGNTEKL
                     LGDCTLDFDSKSVKNKKVFSASRYDDKLLLEKLAWDGMIYRYGKHNAEAAERIGHELE
                     IIDKLGFSAYFLITWDIVRYSMSRGFYHVGRGSGANSIIAYCLKITNVDPIDLNLYFE
                     RFINPKRSSPPDFDIDYSWKERDEVTDYIFRRYGYSHTALLGTINTFRGRSIVRELGK
                     VYGLPKEEIDSLADNPSAESNRNEISDLIFSTGLKIADFPNMRSIHAGGILISEEPIT
                     CYTALDMPPKGFPTTQWDMYTAEEIGFEKLDILSQRGIGHIRESAEIIKRNRAIDVDV
                     HAVHRFKNDPGVKELLRTGETKGCFYVESPSMRGLLQKLKCDDYPTLVAASSIIRPGV
                     ARSGMMREYIYRFHNPGRFEYIHPIMKEQLEETYGVMVYQEDVLKVCHHFAGLDLSDA
                     DTLRRAMSGKYRSKEEMQRIVSKFFSNCRERGYSEEITKEVWRQIESFAGYSFSKAHS
                     ASYAVESFQSLYLKAHFPLEFMVAVINNFGGFYKTWVYIHEAKRWGARIHPPCVNKSN
                     YKTTIYGNDIYLGFIHINGIEEKLARSVETIRNDSGSFSGLNDFIFRANPGLEQVVLL
                     IRSGAFRFTGKKKAVLLWEAHMMVNKGAAESTRQLFPMQARDFALPELASDPLDDAYD
                     EIELFGFPVSLSWFDMLETSFRGEIKAREMMSHVGKRVRMAGHLVTIKYIKTSKGEWM
                     NFGCFIDSEGNFFDTTHFSRSLENYPFRGSGTYLILGKVTEDSGFPSLEAEKLAKLPV
                     KPDKRY"
     gene            complement(18232..>18456)
                     /locus_tag="PK288_05705"
     CDS             complement(18232..>18456)
                     /locus_tag="PK288_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18450.1"
                     /translation="VQQLSMFDDTPEKINLYMTIDKLRRRFGPDTVRRAAGVMTIRER
                     EEKALKQLENALNEQKMMEERLRGYAMHGF"
BASE COUNT         5079 a         4040 c         4395 g         4842 t
ORIGIN      
        1 gcgcggatgt tcggtcagtg atatcgtcag tatgattgcc attaccgcaa atcaggcagc
       61 cggaaaaaag tagttgcatt taattttaat atttccagta acataaaatg atcattctcg
      121 tactcaattg tggcagctcc tccataaaat accagttatt cgatatgagc aacggctgca
      181 acatgctggc aaaaggattg cttgaacgta taggccttag taacagtgtt ctgactcata
      241 aacctaccgg gaaagaaccc tatactgtaa caaatgacat tcctgatcat accacaggca
      301 taaatatggt aatggaggcc ctgaccgacc cgggacacgg tgttatcgcc gatataggcc
      361 agatcaaggc cgtaggccac agggtcgtaa atggagggga aatctacaag gaaagcattc
      421 tggttgacaa cgaggtcaag aagcagattg aacttaacag cgaactggca ccgctgcaca
      481 atcccgccaa tctgaagggc atactttcag tcgaacaact tatccccggt gtcccccagg
      541 ttgccgtttt tgacacctca tttcaccaga cgatgcccga ttatgcatac atgtacgcat
      601 tgccctacga atattatgaa aaatacaaga taaggaagta cggctatcat ggcaccagcc
      661 ataaattcgt tgcttccaag gcagcccgcc tgatggggaa ggatgtcagg gatctcaaga
      721 tcattacctg tcacctcggg aacggagctt ccatcacagc aatacagtgg ggccgttcca
      781 tcgatacctc aatgggattt acacccgtgg acgggctgat aatgggtaca aggactggtg
      841 aaatagatcc cggcgttctt atctacattg cagataaaga acaccttaac gtaaccggaa
      901 taaacaatat aatcaataaa aagagcggtg ttgcaggaat atcacagctg tcgtctgata
      961 tgcgcgatct tgaaattgca gcagcagaag caaatgaaaa ggccatcctt gcactgaaca
     1021 tgtatgcata cagggtcaag aaatacatag gatcgtatat cgctgccctt aacggccttg
     1081 atctgctggt attcaccggc ggaataggag agaacgacca caaaatgcgg gcaatgatct
     1141 gctcagacat ggaaaacctg ggtatccttt ttgatttcaa ggctaatgac ggtgtgagag
     1201 gaaaggattt cgtgatttcc agacccgaat cgaaagtgaa ggtcatgtgc attacaaccg
     1261 acgaggaatt cgtaattgcc tccgacacca ggtctattgt tgagcatcat tccgtatgaa
     1321 ccctcttggt acaaaaccac aaacaagcat gaaaataaaa ttcattcggg ttctatgtga
     1381 accacttaat cctcagtagt atatgaattt attttcacat gaaaaaaacc tacaaatgaa
     1441 aatgatatgg ttctgaaaag tctttccgat ttaaaaggca ttgtttcggg taaacccagg
     1501 aagaaacttg tgcttgccgc tgcacatgac cagcattcgc tgggggcagt aatacaggcc
     1561 tggcatgatg atatagtaga acccattctg atcggtgatg cccgggccat ccagaatatt
     1621 tgcatggaat atggttataa tatagacgga ctgcaggtaa tccatgaacc tgtcaccgaa
     1681 atgtcggttg aaagggcggt caggatgctg aacgggaagc aggctgatgt gcttatgaaa
     1741 ggaaaagttg ggacaacaat tctgctgaaa tgtgtgctta accgggaatg gggcctcagg
     1801 accggcaatc tcctttcgca tattgcgctt tttgaggttg agacttacca caagctgatt
     1861 gccgttacgg atgtcgccat gaatattgct cccaatctta aggataaaat agctattgta
     1921 aacaattcgg tgtcatttct ccacaagctg ggatatgtta tgccgaaagt ggctgtgctg
     1981 ggtgcagttg aaatggtaaa tgagaatatg gaagcaaccc tcgatgcagc ccttctttct
     2041 aaaatgaatc agcgcgacca gatcaaaaac tgcatcattg acgggccatt ggcctttgat
     2101 aatgccataa gctatgaaag tgcaaaacac aagggtatca gaagtgaagt tgccggagat
     2161 actgaccttc tgctgatgcc tgacattgag gttggtaacg tgctttacaa atcactggtt
     2221 ttttttgcaa gggcaaaagt ggcctcgatt atcctgggtg cgcaggtccc gatcgttctg
     2281 acttcccggt ccgattccga acaggccaaa tacgacagta tcctgctttc agctgcagcc
     2341 agcaactaat atcttatgat acgctcactt gaccagatgg tggcaaaggt gctttcgggt
     2401 ggcagaaagt acagaattgc agtcgcatgg gcacatgatg acaataccat ccttgccatc
     2461 agaaaagcgg tagaggaagg gatcgcagag gcattgctta caggaaggaa aaaggagatc
     2521 ctgaaaaact gtcaggcatg gggaatcaaa ccggaaaatt tcacgattat tgaatcggac
     2581 gatgaagttt cggcctcggc agatgccgtt aagctggcaa aaaccgggga cgctgatata
     2641 gtaatgaaag gacttgccgg aacctcccac tttctgaagg ccgtcatgga caaggagaag
     2701 ggtctgatgc ttccgggggc tgtattgagt tatgtgggcg cacttcagct gccagcgtac
     2761 aataaacttc ttttcattac cgatccggcc gttattccct ttcccggcct tgaacagaag
     2821 atcgcaatgg ctgaatacgc gattgaaatg acttcccgtt tcggtattga aaaacccaaa
     2881 atagccctga tcggggcctc ggaaaaaccc agccgtcatt tcgaaagctc ggcacattac
     2941 gaggcaatga aaaaaatggc agcagccggt cagatcagta cctgcataat ggatggacca
     3001 ttggatatct ttcttgcgtg cgacagaaaa agcgttgaaa taaaaggagt taaaacacct
     3061 gtagacggag atgcggatat tctcctgttc ccgtctctgg aatcatcaaa tccgttttac
     3121 aaagggctga tgctttttgc aggcggtgag ctgggaggcc tgattcgtgg aacggaaaaa
     3181 cccgtggttc ttatgtcgcg cagcgaaagt gaaaaatcga agttctactg cattgccctt
     3241 gcatgtttca tggcatgagt ctgccagcct gtacattcag gtcaacaatt acttcaggac
     3301 atggaaaaaa tattaattct tgctgtaaat cccgggtcga cttcgacgaa gtttgcagta
     3361 tttgaggaga acaggcttct gtttgaaaag accttgagac atacacccat ggaactggaa
     3421 ccttacagga aggtcacgga tcaacttcga ttcaggaaag atatgattgt taaagaatta
     3481 acagcaagaa atgttgattt taccaggata agggcaatag tcggacgcgg gggtcttgtt
     3541 aaaccaattg aatcaggtat atatgaggtc aacgacagga tgaggcgaga ccttgaaacc
     3601 ggaataatgg gagagcatgc gagtaatctc gggggactga ttgcggctga cattgcatcg
     3661 tcactgcccg gagcaaaggc atacattgtt gatcccgtcg ttgtcgacga gcttcagccg
     3721 gtagcaagga tatcgggcca tcctgcaatt gagaggatct cgatttttca tgcccttaac
     3781 cagaaggcgg tgggaagact atatgcatca tcggccggac ggagatatga ggatatgaac
     3841 ctcatcattg cacatatggg cggagggata agcgttgggg cacacagaaa gggcagggtg
     3901 atcgacgtaa acaatgctct taacggcgac ggttcgtttt cgcctgaaag atcgggcggc
     3961 cttccttcgg gacagcttgt tgatctgtgc ttcagcggga aatactccca ccaggaaata
     4021 aaatcgatgc tgaccggcaa aggggggatg atggcttacc tggggacaaa caatttcatt
     4081 gaaatgtgcg acagggccga tcagggtgat aaggaggcca tccttatcag ggacgccctg
     4141 gcctatcagg tctccaggga catcggcgca atggctgccg tattggaggg acaggttgac
     4201 gctatcattc tgacaggagg acttgcctac catgaagaac atgttcagcg cataaaaaag
     4261 atggttggat ttatcgcagg aatttatgta tttcccggtg aagatgaaat caaggccctg
     4321 gcatttaacg gattgctggc actggaagga aaaatcgaga ttaagaccta tgtttaggga
     4381 tccgcaattg caaccggtag tattttcccg ttgaaaaact tattgccgct gacggcgaaa
     4441 tatgcaatga atccggccat ttgttccggc attacaggtg cattcatgcc gggaaatgcg
     4501 gtcccgaaca tttcggtctg aacagcgccg agagcaaggc aattaacact gatccccgaa
     4561 ccagagagtt ctgatgcaag acactccgtg agacaggcaa ttgaggcttt tgaagcactg
     4621 taatacgaca gtcccctgta tttggaactt ccctggaatc cccccatact tgagatattt
     4681 acaatgtgcg acccttcgcc catcagcgga acaacaagct ttatcaaagc tgcaggaccg
     4741 aaaaagttta tttccatcat catcctggct tcactgtcgg ttatatccat aaaatccttt
     4801 accgtgagcg aaccggcaca gtttatgagt atgtcaatcc tgctgaagcg gctgaggatc
     4861 tgctttttta tggacggaag cctgcttgcc atgtctgcca gatccattac catgtatgat
     4921 acgttcccgt ggggcaatga atctgcaagt tctttaagag caggttcatc cctgcctgtg
     4981 gcaaggatct ggttatcctt gtcagctgcc atgattctga caatttctct tcctattccc
     5041 tttgatccgc cgctgatgag tatattcatt tttatgaggt tttaattgga atcaggtttg
     5101 ctgctgcctt gaattgttac cggcatgatg cctgagatat tttatggcat tccagatttt
     5161 cccttttccg tcaggtattg ctccccgaaa gttaataaca ggaactgact tttatattgt
     5221 ccagcttatt ttaatcaaga taagaatatt ttatttattt ttcctgttcc ttattttttc
     5281 aatgattaaa attggagttt ctgtataaaa tctgcaagcc gttgtatgca atccgcaaga
     5341 ccggattaat attcttcctg gtcattttgt cggcagttgc ggtttccggg caggagaaaa
     5401 gcaaagacaa gccccccctg tctgaaagat tgtttttcgg aggaaacata ggactggcat
     5461 ttgggactat taccgatatt gaactcactc ctttggcagg tatatgggta ctcccgaggt
     5521 taaatatcgc agcaggaccg accttcaggt attacaagca tccatattat ggcaagacca
     5581 tgatttatgg gggaaggaca tatatggaat tcatgtttct ccaggatatt gataacctgg
     5641 taccccttgg aatacactcg ggcctgttca ttcacgggga atatgagctg ctgagcctgg
     5701 aaacggcttt tttcggcgaa tcctttaata cccggagatt tcccactgga acttttcttg
     5761 ccggaggcgg tataagccag tatctttccc agcgctcctc gcttaatatt agttttctgt
     5821 gggcattgaa tgatgcagga tacggattat attcaaatcc tgaaatacgt atttcattca
     5881 ttttctgaaa tgattcagga taaccggaag gtttccccct gcaccaggca tttcagtgaa
     5941 caaggcacag gcagttacca atgtgcagga gtctcgattc aggaagatca gacgttcagt
     6001 tgctcacctt cggatttggc cacaataacg gctgcacatg tatcaccgaa aacatttatt
     6061 gtcgtcctga acatatcaag aatcctgtca accgccagta tcaggccaat accctcaagg
     6121 ggcaggccca cagccctgag caccaccacc atcattacca gtccggccat aggtatgccc
     6181 gctgaaccga tagctgccag cagtgcagta aagataacaa tcagctgctt tccaaaacta
     6241 agatctattc cgtaagcctg tgcaatgaaa aggactgcca cgcattcgta aagcgcggta
     6301 ccgttcatgt tgacagtggc gcccaacggc agagtgaagc tggctatttt gttcgacact
     6361 ccgtccttgt tttcaaccgc ttccatggtg agcggcaggg ttgcatttga tgacgaggtg
     6421 gaaaaagcag tcagtagcgg cgtggccatg tttctgaaat gcttcaccgg atttgccttt
     6481 ccaaacagtt ttagactgac aggcaatgtt atcagcccat ggaacagcaa acccgccagt
     6541 accgtaatga cataaagccc gagtcttccg acaacctcac cgatattgtt ccccatctct
     6601 acctgctgtg ctatcacctt ggctgttatg ctgaaaatgc caagcggtgc aaacctgatt
     6661 ataaaaagag tgattttcat tattacggct agagcggcat tgaaaacatc cataagggta
     6721 tccctctgct tttcgccaat ctttgtcatt aagaaaccga acagaatgga gaaaaatatt
     6781 attgacagca tctgaccctg agccagtgct tcgaaaatat tcatgggtac aatcttcaca
     6841 agagtctggc caaatgaatc cgatgcaccg gcaatgccct caacggaggc tttgaatccc
     6901 agatcggcac cttcgccggg ttttatggca gttacaagga taaacccggt gagaatagct
     6961 gccagggtgg agaatacata atatcccatc gttttcaatc caagtcgtcc aaggtttcca
     7021 ctgctgccga cacttgcaac ccctgttgtg atcgacaaaa gtataagcgg aacaatgatc
     7081 atgttgaggg cccggagaaa aagatcaccc atccagttcg tatacgttgc tgcgggtgtg
     7141 aaataataac cgaaaaagcc tccggccaga agcattatga gaatctggta gtggagcggg
     7201 agagacagcc tgggttttga catgaatatg aaaattacgg cttaaagaaa cagaaacttt
     7261 gtgagaacag gttataaagc agattaattt ttccggaatt ttaacttctc ctgcagctca
     7321 aaatcggata ctgaccggat ttattccgca aatatcgcaa aattagcggg caaagagtaa
     7381 aaaagcagaa taagaccgaa cataaccttg aaattacaat tgtaaataca ataaaaatta
     7441 taagatcctt acagatgtaa acacaaagct tcaattaatg acattacaaa tgtaaatttt
     7501 tcttcaataa aatattcttt tgatatataa gtaatataat cagctattta aataaattca
     7561 agtgaagtat ctctttatat ggagccacac gatttctgtt caggttttta gaaattaatt
     7621 tatttcttat atataagcca aatactcagt aatatatata tgctaatcta tagatataca
     7681 ttatgaaaat tcaatatata tcagccctta ttcagcaagt tattgaggaa atacagttta
     7741 cgttgttaca ttgattcttt caaaaattac aattgtgaat tacataacat tagatatgta
     7801 atcccaatat tatttacatt tgtaataaac cggtagctat gcttttaagg gaacgaatac
     7861 aggattttct gaaaaaagag aataaaacat cggcccagct ggctgaggaa atcggggttc
     7921 agccatcggg tatttcacac atcctctccg gcaggaacaa tcccagtctt gattttgtca
     7981 tcaggatgct tgaaaaatat ccatttcttt cgactgaatg gctgcttttt ggtaggggat
     8041 ccatgtataa gggtcatttt gccgggtcat tgttcgatga acctcccggc gatgacgata
     8101 aaaccggatt ggcgccagca ggcgcagcaa aacaggctgc ggatgatatc cggttgtatg
     8161 atgagcagga tgataaagca gaatcggaag gggacaggca aaaaccgccc gggaaaagat
     8221 taaggaggat agtctggttt tatggtgaca acagttttga agaattcttt ccggggggag
     8281 actgatctgt tcatttgaaa acaatttttg ataatctttt ctatctttgt tccaaacacc
     8341 tgcttatgcc gaagcgaatt gaggattttg tcatccgtgg gaacaaaagg cttaacgatg
     8401 atttttttgt tctggaactt gctgccggca cccctcttcc cgacatcaac ccggggcagt
     8461 ttgccgaggt gaaggtggaa ggcagtcctg aaacgtttct ccggcgtccg ttttcaattc
     8521 atgatgtcga ttacagggaa aataccatca agctgctcat acagattgca ggaaaaggca
     8581 cggaaaccct ttccggactg aggcccggaa actcactgaa cctggtttat cccctgggca
     8641 attcctttac cataccttct gcaggcgaaa aggttcttct ggttggtggc ggctgtggta
     8701 tagctcctat gctctacctt gcgaggaaga ttgcccaggc aggcattgtg ccaggcatac
     8761 tcctgggatt tcgcaataaa aagcgtgtaa tcgaatatga ggattatcag gagattggag
     8821 atgtttacct tacaacggag gatggatcgg acggagtaaa agggcttgtc accggccatc
     8881 ctgtgctgat gaatagtgtt tttgacagga tgtattgttg cgggcccgaa cctatgatga
     8941 aagcggttgc aaggcattgc ggaaatgccg gcattgcatg cgaagtctca ctcgagaacc
     9001 tcatggcatg cggtataggc acatgccttt gctgcgttgt ggatacagtc aggggaaacc
     9061 tgtgtacctg tacggacgga ccggtattca acacaaagga tctgaaatgg taaatctcgg
     9121 aatatcaata ggaaacctga aatttaaaaa tccggtgctg acggcttcgg gaacattcgg
     9181 gaacgggact gaatttgaag atttttttga tgtttcccgc ttgggaggga ttatcctgaa
     9241 aggaaccaca atcgagccaa gggaaggcaa tccgtacccg agaatggctg aaacgccttc
     9301 gggcatgctc aatacagtcg gattacagaa taaggggatg gattatttcg aaagaaatat
     9361 ataccctaaa gtatcacttt ataaaacaaa tgtaattgca aatataaatg gtagttatat
     9421 cgaagattat gtagcacttg ccgaacgatt tgacaaactg gggaaaatcg ctgcaatcga
     9481 actgaatatc tcgtgtccga atgtcaaaat gggaggaatg gtttttggca ccgatccggc
     9541 atcctccagg aaggttatcc gggctgtacg tgaagcatat tcagggacac tgattgtcaa
     9601 gctttcgcca aacgtaacca gaattgaaga tttcgcgcgg attgccgaag aggagggagc
     9661 tgatgcgatt tcgcttataa atacccttct cggaatgtca attgacattg aatccatgaa
     9721 gccttccctt tcaacgataa ctggcggact ttccggtccg gccgttaaac ctgtagccct
     9781 cagaatggta tggcaatgtg cgcgggccgt aaaagttcca attattggaa taggcggtat
     9841 aatgaacgca tcagatgcca ttgagttctt tcttgcgggc gcttcagcag ttcaggtagg
     9901 caccgcctca tttatagatc ccgtcagttc agtaaggatt ctcgagggaa ttgaaaaata
     9961 ccttgaagaa aaaaagatcg gagatataaa agagatcatc gggctgataa acagtatctg
    10021 acagttaccg caaaccagaa agccgggtca aacagaatat tgctgaatat ttcgaaccaa
    10081 tcagggaatt tagttttaaa aaatatattt actcggattt ttactttaaa tttgtagtaa
    10141 gcgaaaccgt aattccactc tccattaacc taataccaag aaaatgacag gaaatattca
    10201 gattgataat ctcgacagga agattctcga tattataacg aaaaatgccc ggattcctta
    10261 ccttgaagtg gccagggaat gtaatgtaac cggtgctgca attcaccaga gggttcagag
    10321 gctcatcaag tcaggagtga taaaaggttc ggaattcaag gttgatccgg tgctggttgg
    10381 ctttaaaacc tgtgcattca tcggagttta tctcgaccat ccgggccatt accgggatat
    10441 tatcaaaaaa ttcagggaga tatcagaaat catcgagtgc cattatacaa caggcaatta
    10501 ctcgttgttc atcaaagtct atacacacga caatgaacat ctccgtgata ttcttaccga
    10561 aaagatccag gtaattccgg gagtgataag gaccgagacc ctgatatcgc ttgaagaatc
    10621 aatcaacagg cagatccccg tgctatcaga aaaataggaa tattgcagaa tgtctgtttt
    10681 tggcaggaat aacctgtttt caattggagg cagtcctgat tcttttaata agcattagaa
    10741 ttttatattg aacattgccg cttccggcac ctgcgaagat aaatgggttg gagggttcgg
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgcaataaa aaaagcagcc
    10921 tgccggctgc tggtgatcca gctggggctc gaaccgttcc gagcgtaaag aaattgccct
    10981 gtgggcaatt ttagcgaagg agacagcctg ccgcgtttgc gggttcgggc gcaataaaaa
    11041 aagcagcctg ccggctgctg gtgatccagc tggggctcga acccaggacc ccatccttaa
    11101 aagggatgtg ctctacctgc tgagctactg aatcgattct gatttttcga ggtgcaaatg
    11161 tagcaaaaaa tgaaacattt caaaagataa tttctttttc caactgaaaa ttattaaata
    11221 ttaccataaa aatcaaaaaa tattattttt ggtatcgtct aaccatgtca aatccggaaa
    11281 aatgggcaat agcattttca gggacaaagt agtgattata accggtgctt cttcagggat
    11341 aggagaggca gcttcaaggg aatttgccag gaaagggagc aaggttgtaa tggctgccag
    11401 gtcgcttgca aaaatggccg cgattgcgga tgaaattaag gcagggggcg gcgaggcctc
    11461 atatattgaa actgatgtca gcaaagaagc cgattgcaga cggatggtgg aatttgctgt
    11521 ttcccgctat ggaaccgtac attatctcat taacaatgca ggcatttcaa tgcgtgccct
    11581 ttttgatgat gttgacctta aggttcttca caggctcatg gatgtcaatt tctggggtac
    11641 agtctactgt accagatacg ccttgccgta tctgataaaa aacaggggat ccctggtagg
    11701 agtatcctcc gttgccggat ttcacggcct gccgggaaga accggttatt cagcctctaa
    11761 atttgcagtg catggttttc tggaaaccat caggattgaa aacctgaaaa agggccttca
    11821 tgtaatgatc atagcccccg gatttactgc cactgaaata cgtaagaatg ccctgcttgc
    11881 caacggatcc gcacagggag aatccccgag gaaggaagaa aatctaaagt cgccggatta
    11941 tgtggcaaag tggatactta aaggcataag aaagaagaaa agaaacaagc tccttacctg
    12001 ggacggccgg cttacggctc ttttccagag aattatacct gcccttgtcg acctgggata
    12061 ctattatgaa atgtcgcgtg aacccgattc tcccctgaaa tagccaggaa aaaaccattg
    12121 ctgccagctg gcaatattta tttttaaccc ggtgttaaat tgcatcggaa atgattactt
    12181 tcatgataca aaattgaatt atacaaaggc caatgccgga aacacagcga ataaacaggg
    12241 cttacagggc attgctccgc acagccggga ataccctgac ccaggatgat gtcagaaagg
    12301 taagaaaggc gctcgactat gccctgaagg catcatcggg aaatacgatg cttaccgggc
    12361 aacccgaggt tattcatgca ctatcggtag ccaccattat ttccggggag atggggcttg
    12421 gtcttacatc cataataacc gcgctgctgc atgattcata cgggaggctt gaagccgacc
    12481 gggcagtgct tgagaaagag ttcgggaaaa aggttgtgga aatactccag ggcttcgccc
    12541 gtgtttccgg gattacctca atgcagtcat cataccaggc cgaaaacttc agaaaactcc
    12601 tcctgagcct tgctgacgat gttagggtaa tactgattaa actggttgaa aggctggaat
    12661 atatgcgtaa cctcgataag gtcccggaga aggagaaact gccccttgca tcagagacat
    12721 atttcctgta tgcaccactg gcacacaggc ttggatacta taatatcaag actgaactgg
    12781 aggacctggc tgtcaaatac ctggaacctg atcagtacaa ttatgtcgaa ggcaggctga
    12841 aacagacaac agcttcgaga aacaggctca tccgcgagtt ttctgcaccg ctgactgaaa
    12901 agctcgacgg cctgggcata aaatacacaa tcaagagccg cacaaaatca attcactcca
    12961 taatgctcaa aatgaaaaac cagggagttg attttgatga ggtatttgat ctttttgccg
    13021 taaggatcat ggtcgacagt gatccggaaa atgaaaagtc cgactgctgg agagtatatt
    13081 ccgtggtcac cgatctgtat cagccaaacc ccagccgtat gcgcgactgg atatcagtac
    13141 caaaatcaaa tggttatgaa tcacttcata tcactgtggt tggcccgagg ggaaagtggg
    13201 tagaggtaca gatccgttcc aggcggatgg atgaaatagc agaaaaagga cttgcggccc
    13261 atttcaaata caagggaata aaaggagagg ggggactcga tacatggctt acaaggatgc
    13321 ggcagatact tgaatcatcg gatagggagg acagggcttt tctcgaccag gtgcggtcgg
    13381 gcctgtatac cgatgaagtt tttgttttca cacccaaggg tgatttgcgg cagctccccg
    13441 caggagcaac aatcctcgat tttgccttcg atatccatac agccgtgggt gcatcatgcg
    13501 tgggaggaaa agtcaacggc aaaaatgtta caataaggca tgttctgcaa aacggcgatc
    13561 atgtttcagt cttgaggtca aaaagccaga aaccgaagca ggactggctc tcctttgtcg
    13621 ttaccagcaa ggcaaagaca aagattaagc aggctctgag tgaagaaagg accaaagctg
    13681 ccgccgaagg caaggaaatg cttatgcgcc gcctgaagaa ctggaagata acctatggcg
    13741 atgccgtgat tcagcggctt atcatccatt ataacctgaa aacggcccag gatctttact
    13801 gcaacatcca gtcgggcaag actgatctgc tccatataaa ggaaatactt ctgaaaccgg
    13861 gagatgattc tgaacacata catacgcacg aagctgcggc tgaaaaggaa aaaaaagaac
    13921 cagccggttc gccctactcg gattatctta taattgaaga cagtattgag ggattggatt
    13981 ataaacttgc caaatgctgc aaccctgtat tcggcgacag tatcttcggt tttgtcacta
    14041 tatccgaagg tattaaaata cacaggactg gatgccccaa tgctcataac ctcatttcac
    14101 gctatcctta ccgggtagtg attgccaggt ggaccaaggc aaaaggagcc ccctcattcc
    14161 atgctgttat taaaataaca ggagtggaag atatcggaat aggcaacaaa attgcagaca
    14221 tcctgtcaga ttacagtgtt accgtcagga acttcagcta tggcatgaat gaaggaatct
    14281 ttgaaggaac actcagcata atggttccca ataacgatgt cctgtatgga ataatcagaa
    14341 ggatatcctc ggtcaggggg gtcatcaaag ccgtcaggca tacaagcgaa cagaattaaa
    14401 tctgaaaata tcttgaatca tgctgttcag gaatctcata tttgtatccc tcgctccggt
    14461 tctgataatt gcgttttata tctaccatag tgacagatat gaaaaagaac ccttaagtgt
    14521 attattaaaa gccttattta caggagcttt gactataata cctgcagtga ttattgaagg
    14581 tcttattgca gagccttccg gggccgggga agggctggca agcgcggggt atacggcctt
    14641 tgtcgtggca gggtttaccg aagaactgat gaagtacctt gcgttttatt tctttttctg
    14701 gcaggaaagg aatttcaacg aaaagttcga cgggattata tatgcagtat atatttccct
    14761 tggcttcgcc gcagtcgaga atatcctgta tgttttcagc ggcggaatgg gcgtggggtt
    14821 cgtgagggca tttacggccg ttccggcaca tgcactcttt ggtatttcca tgggatacta
    14881 tttcggcctc gcacgttttg attcaaggta caggatcctg aaactgatgc tggcattcat
    14941 tgttccgttt ttctttcacg ggctgtatga ttttcttctg atgagcaaat cccaggtttt
    15001 gcttactgtt tttgtcccgg tgttcatata ttactgggca acgggattca ggaaaatatc
    15061 aaagctttcg gatgcctcac cgttcaggga cagccccggc agacgatcat gatctgacat
    15121 catttatcac tgattattag aatataaaag ccagtcatct tatgtgattc attcagttac
    15181 agggttagtg tttaatatct tttatcaggt tttaccggga gctttgccag tttttcagct
    15241 tcaagcgacg gaaatcccga gtcttccgtt accttgccga gtataaggta tgttccgctg
    15301 cccctgaaag gataattctc cagggatcgt gaaaaatgcg tggtgtcgaa gaaattgcct
    15361 tcgctgtcga tgaaacaccc gaaattcatc cattctccct tgctggtttt tatgtatttt
    15421 attgtaacaa ggtggcctgc cattctcacc cttttgccga catggctcat catttcgcgt
    15481 gcttttatct caccgcggaa tgatgtctca agcatatcga accagctcag cgacaccggg
    15541 aatccgaaga gttctatttc atcgtaggca tcgtcgaggg gatcggaagc cagttcgggg
    15601 agggcaaaat cccttgcctg catggggaaa agctggcggg tgctttcagc tgcaccctta
    15661 tttaccatca tatgtgcttc ccacagcagc accgcttttt ttttgccggt gaacctgaaa
    15721 gccccggacc ttatcagcag gacaacctgt tcaagcccgg ggttcgccct gaaaatgaaa
    15781 tcattgagac cgctgaagga tccgctgtca ttcctgattg tttcgaccga acgggccagt
    15841 ttttcttcaa tgccgttgat gtggatgaaa ccaaggtaaa tatcgtttcc gtatattgtt
    15901 gtcttgtaat tgcttttgtt cacgcacgga gggtgaatac gcgctcccca gcgtttggcc
    15961 tcgtgtatgt aaacccaggt tttgtagaat cctccgaaat tgtttattac cgctaccatg
    16021 aattcaaggg gaaagtgagc tttcaggtac aggctctgga agctttccac ggcatatgaa
    16081 gccgagtgtg cctttgagaa tgaatagcct gcaaatgatt cgatctgtct ccatacctct
    16141 tttgttattt cttcactata gcccctttcg cggcagttgg agaagaactt gcttactatt
    16201 cgctgcattt cctccttcga cctgtatttg ccgctcatgg cccttctcag ggtgtccgca
    16261 tccgagaggt caaggccggc aaaatgatgg cataccttga gcacgtcttc ctggtatacc
    16321 atcaccccgt atgtttcctc aagctgctct ttcattatgg gatggatata ttcgaatctg
    16381 ccggggttgt gaaacctgta gatatattcc ctcatcattc ccgagcgggc cactcccggc
    16441 ctgatgatcg agcttgcagc cacaagcgtc ggataatcat cgcatttaag cttctgcagc
    16501 agacctctca ttgaagggct ttcgacatag aaacagccct ttgtctcgcc ggttctaagc
    16561 aattccttaa ctcccgggtc atttttgaac ctgtgaactg catgcacatc aacatcgatc
    16621 gcacggtttc tctttattat ttcggcgctt tcgcggatat ggcctatgcc gcgttggctc
    16681 aatatatcga gtttctcaaa gccgatctct tcggcagtgt acatgtccca ctgggttgtc
    16741 ggaaagcctt tcggcggcat gtcaagggct gtatagcagg taatgggctc ttccgagatc
    16801 agtatccctc cggcatgtat gcttctcatg tttgggaaat cagctatttt cagcccggta
    16861 ctgaaaatca aatccgagat ctcgttccgg ttgctttcag ccgaggggtt gtcggcaaga
    16921 gaatcgatct cttccttcgg gagtccgtat acctttccaa gttcccgcac gatcgacctg
    16981 cccctgaaag tgtttattgt tccaaggagc gccgtgtgac tgtaaccgta tctcctgaag
    17041 atatagtctg tcacttcatc cctttccttc cacgaataat cgatgtcaaa gtcgggaggt
    17101 gagctccttt tcgggtttat gaaccgttcg aagtaaaggt ttaggtcaat gggatcaaca
    17161 ttagttattt tcaggcagta agcaatgatt gaatttgctc cgctgccgcg gccgacatgg
    17221 tagaatcccc ttgacattga atatctgaca atatcccatg taatcaggaa atatgcagag
    17281 aatccgagct tgtcgatgat ttcaagttca tgcccgatcc tttctgcggc ttcggcattg
    17341 tgttttccgt acctgtagat catgccgtcc catgcaagct tttcgagcag gagtttgtca
    17401 tcatatctgc tggctgaaaa gactttcttg ttcttgacac ttttactgtc gaaatcgagc
    17461 gtgcagtcac cgagaagctt ttcagtgttg ccggcaatcc acctgttctc ctcaagtaac
    17521 tgcttcagac gggagggatc catgaaaaat tcatcttctc ctgcgcactg tgacggattg
    17581 agatgactga gcagcatatt gttgtcgatg gcccggaggc tcctgtgaat tggtatctca
    17641 ttttccgagg agaaagtcac cggttgcagc agtaccatgg aattcctgtc ggagggacgg
    17701 gcagaggtca gcaggctgcc gagttcccgg ggttttatac caatcctctc attttccgac
    17761 agttttctgc ccggcctttt gttcagggga tagatcacgt atgattctga gaatacgggt
    17821 gccggaaaag ggacctgtgc cttttcggaa ttgatttttg agaggaattc gttcaactcc
    17881 cgtaatccct tgttgttccg ggctataccg atataccgga gctcattccc gtccctgaat
    17941 tctattcctg ccacgggtct tatgccgttc ctggcgcatt ctcctgcgaa catgggtatc
    18001 gcggtggtat tgtttatgtc ggtgagggca atggccgggg catttgcagc aatggcgcac
    18061 tgcacaagct gttcaacgga catcataccg tagcgaagac tgtaatatga gtggcagttc
    18121 aggtacatat ctcctgtttt atttcattga tccggcgttc ccggaggcta cgggtatcag
    18181 gcgttgtccc gcattggggc cggatgcctg gtgttccggc atattcaggg cttaaaatcc
    18241 gtgcatcgca tagcctctca ggcgctcctc catcattttt tgttcattta acgcattttc
    18301 gagctgttta agggcttttt cctccctttc ccgtattgtc attaccccgg cagctctcct
    18361 caccgtatca ggcccgaaac gccttctgag cttgtcaatg gtcatataaa gattgatctt
    18421 ttcaggagtg tcgtcgaaca tgctgagctg ctgaac
//
LOCUS       DAOOYG010000045        18260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2939_length_18260_cov_6.509091, whole genome shotgun sequence.
ACCESSION   DAOOYG010000045 DAOOYG010000000
VERSION     DAOOYG010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18260
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2939_length_18260_cov_6.509091"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PK288_05710"
     CDS             complement(<1..1116)
                     /locus_tag="PK288_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPM18451.1"
                     /translation="MGDTMNSMQGPGLKRVLRLPALVLYGIILIQPTAPMPLFGAASN
                     LSNGHVVTTILIGMAAMLLTAISYGRMANAYPNAGSAYTYVSREIHPSLGYLIGWGMI
                     FDYVMNPVICVIWASKAAMNFIPEIPFAVFAVFFAVLFTMMNLRGIEASARTNSVIAA
                     GLGVVILLFLGSAIRYLFYHPPVGVSAWTKPFYNPETFSFKAVSTGASLAVLTYIGFD
                     GISTLSEEVHNPRRNILRASVLVCLITGILASIQVYFAQLVWPETTFPEQDTAFVFVA
                     GKAGGQWLFHTINIALLIATIGSGSGAHLGAGRLLYGMGRDNAIPKKFFSAVNPRTRI
                     PSNNIILVGVFVLIGAFTLSYPLGAQLLNFGALIAFMG"
     gene            1340..1528
                     /locus_tag="PK288_05715"
     CDS             1340..1528
                     /locus_tag="PK288_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18452.1"
                     /translation="MSDNEIIDRVLLNANERGSDIIQVEYIIYTESGIRDPIVIGRGL
                     RGFIDEGIGGKSFSGYIQ"
     gene            1682..3193
                     /locus_tag="PK288_05720"
     CDS             1682..3193
                     /locus_tag="PK288_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPM18453.1"
                     /translation="MITFFIGIIVLLAGYFFYSRVAEKIEGIDPNRETPAGKLKDGVD
                     YVPLPWWKVFLIQFLNIAGLGPIFGAIAGAMFGPVAFLWIVFGAIFIGGVHDYFSGMI
                     SIRHNGLSITEIVGIYLGNWVKQFMRGFTVILMILVGAVFVIGPAGILAGITAKPFGI
                     PFWTVVVFIYYFLATMLPIDKIIGRIYPVFGFALLFMALGLIIALFFKGYKIPELSIS
                     NLRNFHSMPERFPIFPIMFITIACGAISGFHSTQSPLMARCITNELYGRRIFYGAMIS
                     EAIVAMIWAAIAMSFYGGAPELNLAMAGQKGNAAAIVNEISNSLLGKIGGILALLGVV
                     AAPITTGDTAFRSARLIVSDFLNYRQEPVRNRLFVSLPIFITGLILTQINFEIIWRYF
                     AWSNQTMAMVVLWAITVYLMKESKFYYIALIPAVIMTAITSTYFLFASETLNLPLKLS
                     VMLGVFVTVFIYSIFEYYRKSINKNTLPNFVSEKLAMEDNSKKNVTVDKLPGR"
     gene            complement(3427..3500)
                     /locus_tag="PK288_05725"
     tRNA            complement(3427..3500)
                     /locus_tag="PK288_05725"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3464..3466),aa:Pro,seq:ggg)
     gene            3785..4783
                     /gene="trxB"
                     /locus_tag="PK288_05730"
     CDS             3785..4783
                     /gene="trxB"
                     /locus_tag="PK288_05730"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPM18454.1"
                     /translation="MEFFKPVEFQNEDKKPAIEKSEKVKCLILGSGPAGYTAAIYAAR
                     ANLKPVLYTGMQMGGQLTTTTEVENFPGYPDGVTGPVMMEDMKRQAERFGAEVRFGLA
                     TDVDFSGSSHKVIIDDNTEIIASTVIIATGATARYLGIDAETRYAGMGVSACATCDGF
                     FYRGKDVAVVGGGDTAAEEAIYLAGLCNKVYMIVRRNVLRASAAMQTRVMNTPNIEIL
                     WEHQAVDLFGENGVEGAVLVRKKGTPEEEIRRIRIDGFFLAIGHTPNSEVFRKFIDTN
                     ETGYIKTIPGSTRTNVPGVFAAGDVQDPVYRQAVTAAGTGCMAAIDAERYLAELPE"
     gene            4912..5298
                     /locus_tag="PK288_05735"
     CDS             4912..5298
                     /locus_tag="PK288_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPM18455.1"
                     /translation="MRTFRKTYRINAGPADVYAALTNPFTIELWSGYPAKMSTEPGSE
                     FSLWDGDIEGKNLGFITGRELTQEWYFGDRPEKSIVVISIKPDGDRSVVRVEQSNIPD
                     EDYDEICEGWDEYYMGAINDFFNPNF"
     gene            complement(5288..5962)
                     /locus_tag="PK288_05740"
     CDS             complement(5288..5962)
                     /locus_tag="PK288_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HPM18456.1"
                     /translation="MKDKERTDQNLILKGYNMLLYFTGSMIMFEPSEECVTDFFSGEL
                     LRTLPVRSRNPRFIEAASMLRNSCTDKNSCREMLQQDFNTLFSGSLLAPPLKSVYFSN
                     DIYPGKETISDFYDSYGWKDRSRYSFPDDNLGVELLFLTLLTDKYITIDDHVSRAELG
                     NEIRRFIEKHILSWISDWCNMVQEKARTLCYKGIAKLIHACVEDVHTIVRPSAETPAP
                     FADLRN"
     gene            complement(6329..7573)
                     /locus_tag="PK288_05745"
     CDS             complement(6329..7573)
                     /locus_tag="PK288_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM18457.1"
                     /translation="MDIFEKIRSNRGALGQYSDVADGYFTFPRLEGEIAPRMKFRGKE
                     VLTWSLNNYLGLANNPEVRKADAEYAAQFGLAYPMGARMMSGQTTSHEKFERMAAEFE
                     MKEAAYLLNYGYQGMVSIIDALVDRHDVIVYDSESHACIMDGLRLHFGKRFVFPHNDI
                     ASCEKQLQRATKLTEESGGGILVITEGVFGMAGDLGKLDQIARLKEKYNFRFLVDDAH
                     GFGTMGANGRGTGEHFGVQDQIDVYFATFAKSMAGIGGFVASTSEVINYLKYNLRSQI
                     YAKSLPMAMTLGAIKRLELIRSHPEYREKLWAIVNALQKGLREAGLDLGRTESPVTPV
                     FIKGGINQVTQLIYDLRENFNIFCSVVIYPVVPRDVVMLRLIPTAVHTMEDVEYTVNA
                     FREIKRKIDNNEYPDELADFNR"
     gene            7831..8652
                     /gene="bamD"
                     /locus_tag="PK288_05750"
     CDS             7831..8652
                     /gene="bamD"
                     /locus_tag="PK288_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPM18458.1"
                     /translation="MRYRIYILLPVIFLVTSCGEYEKLLKSTDYELKKTRAREYYAQG
                     QYVKASELLGQVIHRFRATTEAEELNWLNAQSYYGMKQYDVAGRLYKSLVEMYPYGQY
                     AEEATFLGALCDYYLAPRAELDQENSMNSIEGFRVFITKFSHSPKVEEARKYINELEE
                     RIVEKSYLNARLYYDMKEYKAAITALTNSLKEHSASKYREEMMFLKLNSLFLYAENSY
                     AAKQTERYQSTLDDYFSFMEEYPQSKYLKEVRKIYQETIRFLKIEKVENLANNQQ"
     gene            8655..8993
                     /locus_tag="PK288_05755"
     CDS             8655..8993
                     /locus_tag="PK288_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPM18459.1"
                     /translation="MDYRKSAAPVTTVTRNLDMLTRDTGNIYETVIIVARRANQISVE
                     MKQDLNKKLEEFASFTDNLEEVFENREQIEISKFYERLPKPTLIALQELEDAKIFYRS
                     PESSTTRGKA"
     gene            9000..10218
                     /gene="coaBC"
                     /locus_tag="PK288_05760"
                     /pseudo
     CDS             9000..10218
                     /gene="coaBC"
                     /locus_tag="PK288_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
     gene            10215..11147
                     /locus_tag="PK288_05765"
     CDS             10215..11147
                     /locus_tag="PK288_05765"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPM18460.1"
                     /translation="MRYYLLKGLFLAMGLISCRGQLLSQELNCNVQISAQKIQGSNRQ
                     VFETMQRDIYEFMNNSVWTNNVFSYAERIDCNMLINLTEQLSADEFRGTIQIQLRRPV
                     FNTTYNSTMLNFVDNNFQFRYVEFQPLEFDPNSYRSSLVSVLAYYAYVILGIDYDSFS
                     PDGGTEFYRIAEKIVTNAQNASEPGWKPYDGSRNRNRYWLIKNLLDDEYKGVRRFIYD
                     YDRNGLDRMESSMPEARTNIVESLRHLQGVYRNRPDPYMYLIQIILESKSDELVNIFS
                     SAFPEEKARVVEILNEIDPGNKTKYQKILAANQP"
     gene            11157..12824
                     /gene="recN"
                     /locus_tag="PK288_05770"
     CDS             11157..12824
                     /gene="recN"
                     /locus_tag="PK288_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPM18461.1"
                     /translation="MLVRLSVNNYVLIRELDLSLDKGLTIITGETGAGKSILLGALSL
                     ILGSRADTSVLLDMKEKCVVEGTFKIDGYDLEEFFTSQDLDYDTVAILRREISPSGKS
                     RAFINDTPVNLGVMRELGDRLIDIHSQHQTLMLRNNSFQLGIIDAFASLDTLKDNYSR
                     VFNSYRKLAREYHELAEESEKNKSDVEYYRFQLSQLEQARLKRGEQAELEKEQELLTH
                     AEDIVSALSGASGLFSGEDGPILSKLREARNLISRIRSFIPEGESLVSRIDSAFIELD
                     DLSGEIEKLAAGIEADPEKLKTVNDRLDHLYSLMQKHRVKDADELIDRKEQIERTVSS
                     VATTDERLGEIEKELAVTRNELTSLADELSERRRSALPLIETRMTDMLKQLGMPNARF
                     KISVKQLDDFISSGRDSADFMFSANRNTEPEDIARIASGGELSRVMLSLKSLMTRSSS
                     LPTIIFDEIDSGVSGEVADKVGQILTGMGNYMQVVNITHLPQIASRGGRHFHIYKTDE
                     GDSTITRIKLLSGEERILEVARLLSGSEVTETALRNARELISAGKNK"
     gene            12853..13695
                     /locus_tag="PK288_05775"
     CDS             12853..13695
                     /locus_tag="PK288_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPM18462.1"
                     /translation="MNEGLLKGKKGVIFGALNEKSIAWQVALKARKHGAELIITNSPV
                     AVRMGTIHELASVTDARVITADATSMTDIDNLISESMRHFGGKFDFILHSIGMSPNVR
                     KAIPYEQTNYENMLKTLDISALSFHRILQTAYKYDALNEWGSVVALSYVAAQRTLTGY
                     NDMGDAKALLESIARSFGYIYGRDRKVRINTISQSPTPTTAGSGIHGFESLVDFSERM
                     SPLGNATAEECADYCIALFSDLTRKVTMQNLYHDGGFSSMGMSLKAIEQYNKSFEECD
                     CADK"
     gene            13780..15051
                     /locus_tag="PK288_05780"
     CDS             13780..15051
                     /locus_tag="PK288_05780"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPM18463.1"
                     /translation="MIKLIILIIVYFTFPILIIYMCKRWTILKKLGSIVLAYAFGLLL
                     GTSGILPSGSEGYYLELRGATALPDARLESLLASGTITENDVFVNSIRRTQDLLTTIA
                     IPLAFPLLLFSLNIRKWIRYAKTGFVSMILALISIIVIIVSGFFMFRDMVPESWKVAG
                     MLIGVYTGGTPNMASLSYALDVDPSLFLITNTYDIIIGAVTIFFFITAGPRVFRAILP
                     PFRHTGEVTDTEKAVAEAQSFDDFSGLLNKERLLPLLKALGISALISVVSAGTAMLLP
                     KTAFQPAAILLITSLGILASLIPSINRIEKTFQLGMYFILVFSFAFASMADLKTIFSI
                     GFLGLFAYVMYAYFGSLLLHLLLSKIFRVNADDYLITTTAFIYSPPFVPVIAAALKNK
                     DVIVTGITVGVIGWIIGNYLGVAMGFMLKGF"
     gene            complement(15189..16538)
                     /locus_tag="PK288_05785"
     CDS             complement(15189..16538)
                     /locus_tag="PK288_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPM18464.1"
                     /translation="MKNIFSLLLCLLTVFSLSGQEKKVKTGWKFGGALPAVTFDSNLG
                     FQYGALFEFFNYGDPSVYPKYYDHTYTEVSRFTKGSGIYRFMFETNHLIPGVEWMSDL
                     SYLTDQANHFYGFNGYVSVFNKDWMDTESSAYRSAMFYRFSRNQFRFKNDFVGKLSGD
                     HLKWSAGFALNNFRVGSVDIDKLNKGKKEDKKLPPLSEEPGLFELYRDSLGIINANEA
                     DGGWVNTIKAGLVWDSRDNRPNPMRGIWTEMGIEISPGFLGNDWGFSKFYITHRQYFT
                     LVENDLALALRLGYQTTLSGKVPFFYQSQVITYRLTGYSSEGLGGTSTMRGILNNRIV
                     GDAFVLGNVELRWKPVYFKFLKQDWYLGVNLFYDLGMITKEISLPDDLQSTFSTKMTD
                     YTFSDFFRPGQEKIHQCAGISIMPVMNQNFVIAVDIGKAFNSQDGNIGFAIGLNYLF"
     gene            16760..18016
                     /gene="hutI"
                     /locus_tag="PK288_05790"
     CDS             16760..18016
                     /gene="hutI"
                     /locus_tag="PK288_05790"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPM18465.1"
                     /translation="MALLITNIKGLVGAGAIQRSLVAGKEMSELTTIPDAYIFCRDGL
                     ITDFGQMRDMDPAVAEESGRELITIDASGKFVFPSFCDSHTHLVYAGSREQEFADRIR
                     GLSYEEIARRGGGILNSARLLHATSEDELYEQSSERIREIIRQGTGAVEIKSGYGLNT
                     EDEIKMLKVIRRLRDDFPIEVRSTFLGAHALPDEFRSDREAYIDLVVNEMIPMVAYGE
                     LADYIDVFCDTGFFTVEETERILMAGIKYGMIPKIHANELDYSGGVQTGVRYDALSVD
                     HLERTGDAEIEALLGTGTMPTLLPGSALFLGLPDPPARRMIDAGLPLALASDFNPGSS
                     PSGNMKLVMSLACIRLKMTPEETINAVTLNSAYAMGLSETHGSIAMGKAANMFVTREM
                     PSYGFFPYAFGSDLIETVILKGQIIP"
BASE COUNT         5078 a         4051 c         4323 g         4808 t
ORIGIN      
        1 tcccatgaaa gcaatcaggg ctccgaagtt aagcagctgg gctcccaggg ggtaggagag
       61 ggtgaaggcc ccaatcagga cgaatacccc gacaaggatt atattgttgc tcgggatcct
      121 tgtgcgggga ttgactgctg aaaagaattt ttttggtatg gcattgtcgc ggcccattcc
      181 gtagaggagg cgcccggcgc cgagatgggc acccgaacct gaaccgatgg tggcaatgag
      241 cagggctatg tttatcgtat ggaagagcca ctgtccccct gctttccccg ctacaaagac
      301 gaatgccgta tcctgctccg ggaaagtggt ttcgggccag acaagctgtg caaagtatac
      361 ctggatggaa gccagtatcc ccgtgatgag gcataccagg accgatgccc gcagaatatt
      421 acggcgagga ttgtgcactt cttccgacaa tgtcgaaata ccgtcaaatc ctatataggt
      481 aagcactgca agtgaagctc ccgtggaaac cgccttgaag gaaaaggttt cgggattgta
      541 gaaaggcttt gtccaggcac ttaccccaac cgggggatga taaaaaagat aacggatcgc
      601 cgatccgagg aaaagcagga tcacaactcc aaggccggct gctattacag aatttgtacg
      661 ggcacttgcc tcaatgcccc ggagattcat catggtgaac agaacagcga aaaagactgc
      721 aaatactgcg aaaggtattt cgggaataaa attcatagcc gccttgcttg cccatatcac
      781 acagatgacc ggattcatta cataatcaaa gatcataccc cagccgataa ggtatccgag
      841 cgacggatgg atctccctgc tgacataggt gtaggcggaa ccggcattgg gatatgcatt
      901 tgccatacgg ccgtaactta ttgccgtaag aagcatggct gccattccga tgagaatggt
      961 tgttacgaca tgtccgttcg aaaggttgct cgctgccccg aacaatggca tcggagctgt
     1021 gggctgtata agtattatgc cataaaggac caaagccggt agcctcagaa ccctttttaa
     1081 tccgggtccc tgcatggagt tcatagtgtc gcccataatc ggagattttt gtttacaggt
     1141 tattgattga aggaccaaag atattaaaat ttcattacgt tcgttttgct gctgaaggtt
     1201 caatggaacg ctggtaaaat attatatttc tataaaaaaa gggagtcact aactcctgac
     1261 tcccttccgg tcggggtgat ctgatggata acttatcaca atcaactatt tgataaatga
     1321 ttgtttcttt gtggtaataa tgtcagataa tgaaataata gacagagtac ttttaaacgc
     1381 aaatgagaga gggagcgata tcattcaggt tgaatatatt atatacactg aatccggaat
     1441 ccgagatcca atcgtaatag gaagaggcct tagaggattt attgatgaag gtataggtgg
     1501 gaaaagcttt agtggataca tacaataatt agaacatcaa taaagaataa aaaagaaata
     1561 tttataaata aattagtggc tttataagct gcctggtttt ttattccaga ccaggtcatt
     1621 gatagtcggt tatttgcata aaaaaagttt aaacctgaat tactgatcag ataatattga
     1681 tatgattaca ttttttattg gtataatagt gttattagca ggatatttct tttattccag
     1741 ggtagctgag aagatagaag gtattgatcc aaaccgggaa actcccgcgg gcaaattaaa
     1801 ggatggtgtt gattatgttc ctttgccctg gtggaaagtg tttctgattc agtttctgaa
     1861 cattgctggc ctgggtccga tttttggagc aattgccgga gctatgtttg gtccggtagc
     1921 ttttttatgg atagtattcg gggcaatttt tatcggaggc gttcatgatt atttttccgg
     1981 aatgatatcc attcgtcata acggattaag tattaccgaa atagtgggca tatatctcgg
     2041 aaactgggtc aagcaattca tgagaggttt cactgttatt ctcatgatat tggttggggc
     2101 agttttcgtt atcgggcccg cgggtattct ggccggaatt acggcaaagc ccttcggtat
     2161 cccattctgg acagtagttg tttttattta ttatttttta gcgaccatgc ttcctattga
     2221 taaaatcatt ggacggatct atcctgtgtt tggttttgcc ctcttattta tggccctggg
     2281 attaatcatt gctttatttt ttaagggata taaaatacca gagctgagta tttcaaattt
     2341 aaggaatttt cattccatgc ctgaaagatt cccgatattt cccattatgt ttatcacaat
     2401 tgcatgcggt gcgatttcgg gttttcattc aacccagtct cctttgatgg caagatgtat
     2461 cacgaatgag ttatatggac gcaggatctt ttatggcgct atgatatcag aggctattgt
     2521 ggcaatgatc tgggccgcaa ttgccatgag cttttatgga ggggcgcccg aactaaatct
     2581 agccatggct ggtcaaaagg ggaatgctgc cgccatagtg aatgagattt caaactcact
     2641 cctgggaaag attggaggta tcctggcttt attaggggtg gttgcagcac ctattactac
     2701 tggagatacc gctttcagga gcgccagact tattgtttcg gattttctca attacagaca
     2761 ggaacctgtt agaaatcgct tgtttgtatc cttgcccatt tttataacgg gcttgatcct
     2821 tacccagatt aatttcgaaa ttatctggcg atattttgca tggtctaatc agacaatggc
     2881 catggtggta ctctgggcta ttacggtata tctcatgaag gaatcaaaat tttattacat
     2941 agcccttatt cctgccgtaa ttatgacggc aataaccagc acatattttc tttttgcatc
     3001 ggagactctg aatcttccac tgaaattatc agtaatgctg ggagtatttg ttactgtctt
     3061 tatatactcc atatttgaat attacagaaa atccattaat aaaaatacac tgccaaactt
     3121 tgtttctgaa aaactggcca tggaggacaa tagtaaaaag aatgtcacgg ttgataaatt
     3181 accggggaga taaccttatt ggcgggatat tgcagcctga catccaaagc cctttatgaa
     3241 atagtcactt acggtatctg ttattcattt ttgtgttctg aggttaccgg gaaaccggct
     3301 caggcaagca gaagatagat ttatacagga tcctccggga aaaagggatt gttgaaatag
     3361 tggtgcctat gtggcgaaat aaaaaactca tctacctgat tgcaagataa atgagtttaa
     3421 taaatgtcgg ggtgatctga ctcgaacaga cgaccccttg caccccatgc aagtgcgcta
     3481 gccaactgcg ccacaccccg gtaaatgaat tgcaaaatta agcaacaaat atgattctgc
     3541 aaagtgaaaa attaaaactg atgccagatt gtccggcaga cgtccttttc ccatgttgtt
     3601 attcgggaaa gtttgccccg gagcttttca ccaagcccgt tgctttctgc ctatggataa
     3661 atgcaccggg cctgctgaag attcccggga aagaccggcc ggcatgaaaa tttattttgt
     3721 gacaaaagtc actgaagcag atcaaagggt tattgtaaat ttgtgcaaaa gaaaaactgg
     3781 aattatggaa ttttttaaac ctgttgaatt tcaaaacgaa gataaaaaac ctgctattga
     3841 gaagtctgaa aaggtaaaat gcctcattct tggctcggga ccggccggct atactgctgc
     3901 catatatgca gccagggcga acctgaaacc ggttctctat accggaatgc agatgggggg
     3961 acagctgacc acaacaaccg aggttgagaa tttccctggt tatcccgacg gggttaccgg
     4021 acctgttatg atggaagata tgaagaggca ggctgaaaga ttcggggccg aggtccggtt
     4081 cggccttgcc actgatgtcg acttttcagg gagcagtcat aaggttataa tcgacgacaa
     4141 cactgaaata atcgcttcaa cagttattat tgccacagga gcaactgcca ggtaccttgg
     4201 gatcgatgcg gaaacacggt atgcggggat gggagtgtcg gcctgtgcaa cgtgcgacgg
     4261 tttcttttac agggggaagg atgtggcagt agtcggtggc ggtgatacgg cagccgagga
     4321 agccatttac cttgcaggat tatgcaacaa ggtttacatg atcgtcagaa gaaatgtact
     4381 gcgggcttct gcagcaatgc agaccagggt aatgaataca cccaatattg aaatattgtg
     4441 ggagcatcag gctgtcgatc ttttcggtga aaacggggtt gaaggcgctg tgcttgtcag
     4501 gaagaaagga acgccggaag aagagataag acggatcaga attgacggat tcttccttgc
     4561 aattggccat acccctaatt ctgaagtgtt caggaaattc atcgatacca acgaaacagg
     4621 ttacataaaa acgatcccgg gatcaacacg aacaaatgtc ccgggcgtgt ttgccgccgg
     4681 agacgtgcag gatccggtct accggcaggc cgttactgca gcgggaacag gatgcatggc
     4741 tgctattgat gctgaacgtt atctggcaga gctgcccgaa taagcaacac aggatggtaa
     4801 aatatggcta actatactga caccggtttt tccatgctgc ataatacggc ggaaagaccg
     4861 gggagcttaa cagttaccgg ctgcagtctt taaacaggaa aaaccaagag tatgagaaca
     4921 ttcaggaaga cataccggat caatgccgga cccgcagatg tgtatgctgc tctcaccaat
     4981 ccctttacaa ttgaattgtg gtcgggatat cctgcaaaaa tgtcgactga accgggatct
     5041 gaattttcat tgtgggatgg ggatattgaa gggaaaaacc ttggattcat aaccggcagg
     5101 gagttgaccc aggaatggta tttcggcgac cggcccgaaa aatccattgt agttatcagc
     5161 ataaaacccg acggagaccg gtcggtcgtg agggttgaac aatcgaatat tccggatgag
     5221 gattatgatg aaatatgcga aggatgggac gaatattaca tgggtgccat caatgacttc
     5281 tttaatccta atttctgaga tctgcaaaag gcgcaggcgt ttccgccgac ggcctgacaa
     5341 tggtatggac atcctcaaca caggcatgga taagcttggc aatacccttg taacagagtg
     5401 tccgggcctt ctcctgcacc atattgcacc agtcggagat ccatgacagt atgtgctttt
     5461 ctatgaacct cctgatctca tttcccagtt cggccctgct gacatgatcg tcaatcgtga
     5521 tgtatttatc ggtcaaaagg gtaagaaaca acaattcaac tccaagatta tcgtcgggga
     5581 aactgtagcg cgacctgtcc ttccatccgt aggaatcata gaaatcactg atggtttctt
     5641 tcccgggata tatatcatta ctgaaataaa ccgacttgag cggcggcgcg agaagggatc
     5701 ctgaaaaaag agtgttaaaa tcctgctgaa gcatttccct gcatgaattt ttatccgtgc
     5761 atgaattcct cagcatggaa gctgcttcaa tgaacctggg attcctgctc cgtaccggga
     5821 gggtcctgag caattcgcct gagaaaaagt cggtaacaca ttcttcagag ggttcaaaca
     5881 ttatcatgct cccggtaaaa tagagaagca tattgtatcc tttcagtatc aggttctgat
     5941 cagttctctc cttatctttc atttgaattt tcagacaact cattttagcc ggttaaactt
     6001 atccgggtga agatcacaga aaataaaccc cccggatcaa aaaaagttca atctgcgctg
     6061 aaaatcaata ggtgtttccc gattttttta attgcaggct atgccaggat ggggtgcctg
     6121 agaactgtaa tcccggaggg tcagcccgca ttttatcctg tatttggaaa cagcagaagt
     6181 cagcgtgtat tttactcctg cagtaccgcg tccgactcat ggatcgcccg atcatcacag
     6241 gctaatgttc aggtggcccg gttccttaag caggctgtga tatgcggatg attgccgtaa
     6301 gcagaacagg gaccatcctg cagtggtatt acctgttaaa atccgccagc tcgtcagggt
     6361 attcgttgtt atcgatcttt ctttttattt ccctgaaggc attcaccgta tattccacat
     6421 cctccatggt atggacggca gtggggatca gccgcagcat gacaacatcg cgcgggacca
     6481 ccggatatat tacgaccgag cagaaaatgt tgaagttctc cctcagatca tagataagct
     6541 gggttacctg attgatccct cccttgataa agacaggagt taccggcgat tcggtcctgc
     6601 caaggtcaag accggcctcc cttaacccct tttgaagagc attgacaatt gcccacagct
     6661 tttcgcggta ttccggatgc gaacggatca gttccagacg tttgattgct cccagggtca
     6721 ttgccatcgg gagcgatttg gcatagatct gcgagcggag gttatatttg aggtaattta
     6781 tcacttcact cgtgctggca acaaatcctc ctattcccgc catcgacttg gcaaacgttg
     6841 caaaatacac atcgatctga tcctgaacgc caaaatgttc gccggttccc ctcccgttgg
     6901 cacccatggt tccgaacccg tgggcatcat ctaccaggaa cctgaagttg tatttttcct
     6961 tcagacgggc aatctgatca agtttgccaa gatctcctgc cattccaaac acaccttcgg
     7021 tgattacaag aatacctccg cccgattctt cagtaagctt tgttgcacgc tgaagctgtt
     7081 tttcacagct ggcaatatca ttatgaggga atacaaaacg ctttccgaaa tggagtctca
     7141 gaccgtccat tatgcatgca tgagattcgg aatcataaac tatgacatca tgcctgtcga
     7201 caagtgcatc aattattgat accatcccct ggtagccgta attcagcagg tatgctgcct
     7261 ccttcatctc gaattccgcc gccattcttt caaacttctc atggctcgtg gtttgccccg
     7321 acatcattct tgcacccatc gggtaggcca gaccaaattg cgcagcatat tccgcatcgg
     7381 ctttcctgac ctcgggattg ttcgccagac caaggtagtt attcaggctc catgtcagca
     7441 cttccttgcc cctgaatttc attcgcggag caatctcccc ttcaagcctg ggaaatgtaa
     7501 aatacccgtc ggcaacatct gaatactggc ccagggcacc cctgttcgat ctgatctttt
     7561 caaatatatc cattataaca agctttttaa aagtcgcaca aatgtacaca tttaaacggg
     7621 ttgaagagat acctatttat acctaaattt ttattatagt gcctgtgtca gaaatccgga
     7681 atactttttg cgcacccttc acaggctgcc gcctgaatca gcagctttgg gaacggaaag
     7741 catcaggaag gagcttatca gcggttataa ttttgaattt gttgtatgtg atttcaaaaa
     7801 aaacagtaat tttgcgcaac atttcaaaag atgaggtaca ggatctatat tttgctaccg
     7861 gtaattttcc tggtcacttc gtgcggtgag tatgagaagc tgctgaaaag cacggattac
     7921 gagctgaaaa aaaccagggc ccgggagtat tatgcgcagg gtcagtatgt gaaggcatca
     7981 gaattgttgg gtcaggtgat ccaccgtttc agggccacca cagaagcaga agagctgaac
     8041 tggctcaatg cccagagcta ttacggcatg aaacagtacg atgttgcagg caggctgtac
     8101 aaatcgcttg ttgaaatgta tccctatggc cagtatgccg aggaagccac tttcctggga
     8161 gcattatgtg actattacct ggcgccccgg gccgagcttg accaggaaaa ttcgatgaac
     8221 tcaatcgaag gcttcagggt attcataacc aagttttccc acagtccgaa agttgaggaa
     8281 gccaggaaat atataaatga acttgaagaa cgtattgttg agaaatcgta cctgaatgcc
     8341 aggctgtatt atgatatgaa agaatacaag gcggccataa ccgccctcac caacagcctg
     8401 aaagaacatt cagcctcaaa ataccgcgaa gagatgatgt tcctcaagct caattccctg
     8461 tttctgtatg ccgaaaacag ctatgcagca aagcagacgg aaagatatca gtccactctc
     8521 gatgattact tctcattcat ggaagaatat ccccagagca agtatttgaa ggaagtcagg
     8581 aagatttacc aggaaacgat ccgattcctg aaaattgaga aagttgaaaa cctagcaaac
     8641 aatcagcaat aaacatggat tacagaaaat cagcggcccc ggtgactaca gttaccagaa
     8701 atcttgatat gcttaccagg gacaccggca atatttatga aaccgtaata attgtggcca
     8761 ggagagccaa tcagatctcg gttgaaatga agcaggatct caacaagaag ctggaggaat
     8821 ttgcttcctt caccgataac cttgaagaag tttttgagaa ccgcgagcag attgaaatat
     8881 caaaattcta tgaacgcctt cccaagccga cgctcattgc attgcaggag cttgaggatg
     8941 ccaagatatt ttacagatct cccgaatcgt ccacaacaag ggggaaagct taattttcaa
     9001 tgctgagggg taagaaaata ctggtgggga tcagcggcgg catagcagcc tataagactg
     9061 cgacaattgt ccgcctcctg gtcagggaag gctgcgaggt gaaggtgatt atgaccgacc
     9121 gggcaaagga cttcattacc ccgcttactc ttgcaacact ttctaagaac cccgtttcca
     9181 gcggttttta cctgccagaa agcggtacct ggaacagtca tattgaattg ggaatgtggg
     9241 ccgacttgtt tcttgttgcc ccggcaactg ccaatacaat ctcaaagatg gcaaatggtg
     9301 cggctgataa tctcctgctt gcaacttatc tttcggcacg ttgcccggtt ttcattgccc
     9361 cgtcaatgga ccttgatatg ctgaaccatc ctgcggtcag aaagaatctg gaaatcctgg
     9421 gttcctatgg taatcatata ctcgattcac ccgacggcga gctggcaagc ggccttacag
     9481 gcaagggaag aatggctgag ccggaggata ttattgagga actgaaaagc ttcttttcaa
     9541 aaaaaaaaat cccttaaagg caagaaaatc ctgatcaacg ccggccccac acaggaatcc
     9601 attgacccgg tgaggtatat cagcaaccat tccaccggca aaatgggaat cgcactggcc
     9661 gatgctgcat gtgaaatggg cgctgaagta cagcttgtac tgggtccggt cagcctgcgg
     9721 ccacgaaaca ggtcggtcag ggtaataaat gtcacaacgg cccggtcaat ggctgatgaa
     9781 tgtatttcaa ggttccgcgg ctcggatgta gccatcctgg ccgctgcagt tgccgactac
     9841 accccggttg atacgcccgg aaaaaagctc aagcgaggac caggcgacct ggtgatcaga
     9901 cttaaaccaa ccgaagatat tgccgccata ctgggccggt caaaaacaga cagacagatt
     9961 gttgcaggtt ttgcccttga aaccgacaat gaaattgaaa acgctaaaga taaactccgg
    10021 aggaaaaacc ttgatattat tgttctgaat tcactgaagg acaaaggctc aggatttgga
    10081 catgacacca accggattac cattattgac aagaacaata atattgataa atttgaactg
    10141 aaaacaaagg aagaggcggc aggggatatc ctctccaaga tcgttgcaat gatgaacatt
    10201 cagattcagg ggttatgaga tattatttac tgaaaggcct gtttcttgcc atgggcctga
    10261 taagttgccg ggggcaactc ctcagccagg aattgaattg caatgttcag atctcggctc
    10321 agaaaataca gggctcgaac cgccaggtct ttgagaccat gcagcgcgac atctacgagt
    10381 tcatgaacaa ttcggtatgg accaacaatg ttttcagtta tgccgagagg atcgactgca
    10441 atatgctgat aaatctcact gaacagttgt cagctgatga attcaggggt accatccaga
    10501 tccagttgcg ccggcccgtg ttcaatacca cctacaattc gacaatgctg aatttcgtgg
    10561 acaacaattt ccagttcagg tatgtcgagt tccagcctct cgagttcgac cctaattcat
    10621 acaggtcaag cctggtttcc gtgcttgcct attatgcata tgtcatcctc ggaattgatt
    10681 atgattcctt ttccccggac ggaggcacag aattttacag gatcgccgaa aagatcgtga
    10741 ccaatgccca gaatgcctct gaaccgggtt ggaaaccata cgacgggtca aggaacagga
    10801 acaggtactg gctcattaag aaccttcttg acgatgaata caaaggtgtg aggagattta
    10861 tctatgatta tgacaggaac ggccttgacc ggatggaatc aagcatgcct gaagcccgga
    10921 caaacattgt tgaaagcctc aggcatctcc agggagtgta cagaaacaga cccgaccctt
    10981 atatgtacct gatccagatt attctggaat ccaaatcaga tgagctggtg aatattttct
    11041 ccagtgcttt tcccgaggaa aaagcccggg ttgtcgaaat acttaacgaa atcgatccgg
    11101 gcaataagac aaaatatcag aagatcctcg ctgcaaacca accctaagag aaggttatgc
    11161 ttgtaagact ttccgttaac aattatgtgc ttatcaggga gctcgacctt tctcttgata
    11221 aaggtcttac aattattacc ggtgaaacgg gtgccgggaa atcaattcta ctgggcgcac
    11281 tgtcactgat ccttggttca agggctgata catccgtgct cctcgacatg aaggaaaaat
    11341 gcgtggtgga aggcaccttc aagattgacg gctatgatct tgaagagttt ttcacctctc
    11401 aggatctgga ttatgatacg gttgccattc tgagaaggga aataagtcca tcaggtaaat
    11461 caagagcttt cataaacgat acacctgtta acctgggggt gatgagggag cttggggaca
    11521 ggctcattga tattcattcc cagcaccaga cactaatgct caggaacaac agcttccagc
    11581 tgggcataat tgatgctttt gcttcacttg acaccttgaa ggacaactac agccgtgttt
    11641 ttaactcgta ccggaagctg gccagggagt atcatgaact ggcggaagaa tcggaaaaga
    11701 acaaatccga tgttgagtat tacaggttcc agctttcaca gctggagcag gcacggttga
    11761 aaagagggga acaggccgaa cttgagaaag agcaggaact gctgacccat gcagaagata
    11821 ttgtttcggc actgtcaggc gcttccggcc ttttttcggg cgaagacggc ccgatcctat
    11881 caaagctcag ggaggcaaga aaccttatct cccggataag atcgttcatt ccggaaggag
    11941 agagtcttgt ttcaaggatt gattctgctt ttattgagct ggacgacctg tcaggcgaaa
    12001 tcgaaaagct tgcagctggc atagaagcgg atccggaaaa gcttaaaacc gtgaatgaca
    12061 ggctcgatca tctgtattcc ctcatgcaga aacacagggt taaagacgct gacgaactga
    12121 ttgacaggaa agaacagatt gagagaacgg tcagttctgt tgcaacaacc gatgaaagac
    12181 tgggtgagat tgaaaaagaa ctggccgtaa cccgtaatga gctgacctcc cttgccgacg
    12241 aattatctga aaggcgaaga tcggctctcc cgcttatcga aacccgtatg acggatatgc
    12301 tgaaacagct tggcatgccg aatgcccggt ttaaaatatc agttaagcag ctcgatgatt
    12361 ttatttcctc gggaagggat agcgctgatt tcatgttttc agcaaaccgg aatactgaac
    12421 ctgaagacat agcccgaatc gcttctggag gagaactttc cagggtcatg ctgagcctta
    12481 aatcactgat gacccggagc agcagcctgc ccaccattat attcgacgag attgattcgg
    12541 gggtttcggg ggaggttgca gacaaggtgg gccagatcct taccggcatg gggaattaca
    12601 tgcaggtggt aaatatcacc caccttccgc agattgcttc aaggggaggc aggcattttc
    12661 atatttataa aactgacgag ggtgattcaa caattacacg cattaaattg ttatcaggtg
    12721 aagaacgaat cctggaagtt gcaaggttac tgagcggtag cgaggtgacg gaaacagcct
    12781 taagaaacgc gagggagctg ataagtgcag gaaagaataa gtaacatttg gtattgtaaa
    12841 tgatcgcttg aaatgaatga aggtctttta aagggtaaaa aaggtgtgat attcggtgcc
    12901 ctgaatgaaa aatccatcgc atggcaggtt gccctcaaag cccgtaaaca tggtgcggag
    12961 ctgataatta ccaattctcc tgttgcggtg cggatgggta caattcatga actggcttca
    13021 gtcactgatg ccagggtaat aacggcagat gccacaagca tgacggatat tgataacctt
    13081 atttccgaga gtatgaggca cttcgggggt aagtttgatt ttatccttca ttcgataggc
    13141 atgtcgccaa atgtgcggaa agccatacca tacgaacaga ccaattatga gaacatgctg
    13201 aagacccttg acatttcagc actcagcttt cacaggatac tgcagacagc ctataaatac
    13261 gatgcactca atgaatgggg ttcagtggtt gccctttcct acgttgcggc acagcggacc
    13321 ctcaccggct ataacgacat gggcgacgcc aaggccctcc tggaatcaat tgccagatcg
    13381 ttcgggtata tttacggacg cgacaggaag gtgcggataa acacaatatc ccagtctcca
    13441 actcccacaa ctgccggaag cggaattcac ggattcgagt cgctggttga cttctcggaa
    13501 agaatgtcac cccttggcaa tgccacggca gaggaatgtg ccgattactg catcgccctc
    13561 ttctccgatc ttacccgcaa ggttaccatg cagaatcttt atcacgacgg cggattttca
    13621 agcatgggaa tgagcctcaa agccatcgaa cagtataaca agagctttga ggaatgtgac
    13681 tgtgcagaca agtaaataag tataaatacc tattttccag gcggtttgca ttattgtgaa
    13741 ccgctttttc tatatttggg aatacctaaa acctgtcgta tgataaaact tatcattctt
    13801 atcatagtgt acttcacttt tcccattctt ataatttaca tgtgcaaacg ttggacaatt
    13861 ctgaaaaaac ttggttcaat agtgttggca tatgcatttg gtttgctcct gggaacatca
    13921 gggatccttc cctcaggcag cgaaggctat tatcttgaac ttcgcggagc cactgccctg
    13981 ccagatgcaa gattggaatc acttcttgcc tcgggaacaa tcactgagaa tgatgttttt
    14041 gtgaattcca taaggagaac ccaggatctg cttacaacaa ttgcaatacc actggctttt
    14101 cccctgctgt tattttcact taatatcagg aaatggatca gatatgcaaa aacgggcttt
    14161 gtaagcatga tactcgccct catttcaata attgttatta tagtctccgg attcttcatg
    14221 ttcagggata tggttcccga gagctggaaa gttgccggca tgcttatcgg ggtctataca
    14281 ggagggacac caaacatggc ttccctcagc tatgcactcg atgtggatcc aagtcttttc
    14341 cttattacaa acacttatga tatcataatc ggtgcagtta caatcttttt tttcattacg
    14401 gccggtccac gggttttcag ggccatcctt cctcctttca ggcataccgg cgaagttaca
    14461 gatacggaaa aagctgttgc cgaggcacag agttttgatg atttttcggg cctgcttaac
    14521 aaggaaagat tgctcccctt gctgaaggca cttggaatat ccgcgctgat ctccgttgtt
    14581 tccgccggaa cggcaatgct cctgccgaaa acagcattcc agcccgcagc tatcctgctg
    14641 ataacctccc tcggtatcct ggcttcactg ataccatcga tcaacaggat tgaaaaaaca
    14701 ttccagcttg gtatgtattt catccttgtg ttttcctttg cctttgcatc gatggccgat
    14761 ctgaaaacaa tattcagcat tggctttctc ggactgttcg cttatgtaat gtacgcctat
    14821 ttcggctcac tgcttcttca cctcctgctg tccaaaattt tccgggttaa cgcagatgat
    14881 tatctcatca caaccactgc cttcatatat tcaccgccgt ttgtgcccgt aatcgccgct
    14941 gcgctgaaga acaaggatgt tattgttacc ggaataacag taggagttat cggatggata
    15001 atcggtaatt acctcggggt ggccatgggt tttatgctga aaggattcta gggggcacgc
    15061 aatccataat actgtcctgc tgcccctggt gagggatgtc ccaggttaaa tggtttttgt
    15121 gatgtcaggt gcatgccccg ggtgttactg tgctgcccgg gttattcagt gaataattgc
    15181 aactgatgct aaaacaggta attcaatcct attgcaaatc caatgttccc gtcctgtgag
    15241 ttgaatgcct tcccgatatc gactgcaatc acgaaattct gattcatcac cggcattatg
    15301 ctgatacctg cacactgatg gatcttttcc tgaccgggcc tgaagaaatc agagaaagtg
    15361 taatctgtca tttttgtgct gaacgtgctt tgaaggtcgt cgggcaagga gatttccttc
    15421 gtgatcattc cgaggtcata gaacaggttc acaccaaggt accaatcttg tttcaggaat
    15481 ttgaagtaga ccggtttcca gcggagctca acattgccca gcacaaatgc atcacccact
    15541 atcctgttgt ttagaattcc cctcatggtg ctggtccctc ccaatccctc gctcgaatag
    15601 cctgtcagtc tgtaggtaat aacctgcgac tggtaaaaga aggggacttt tccggaaagc
    15661 gttgtctgat atcctaacct cagggcaagt gccagatcat tttccacaag tgtaaaatat
    15721 tgccggtgtg ttatatagaa ttttgagaat ccccagtcgt ttccaaggaa tccgggcgat
    15781 atttcaattc ccatttcagt ccatatacct ctcatcggat tcggtctgtt atccctgctg
    15841 tcccacacca ggcctgcctt tatggtgtta acccagccgc cgtcagcctc atttgcattt
    15901 attatgccga gagaatctcg gtaaagttca aaaagtccgg gctcttctga aagcggcgga
    15961 agctttttat cttctttttt ccccttattg agcttatcaa tatccaccga acccaccctg
    16021 aagttgttca atgcaaaccc tgcactccac ttaagatgat cgcctgaaag cttccctacg
    16081 aagtcattct tgaagcggaa ctggttcctg ctgaaacggt aaaacatggc actcctgtag
    16141 gcggatgatt cagtatccat ccagtctttg ttaaataccg acacatagcc gttgaaaccg
    16201 taaaaatgat ttgcctgatc ggtcaggtaa ctgagatcgc tcatccattc cacacccggg
    16261 ataagatggt ttgtctcgaa cataaacctg taaatgccgc tgcctttggt aaatctggaa
    16321 acctcggtat aggtatgatc ataatacttc ggatacacgc tcggatcacc ataattgaag
    16381 aattcaaaaa gagcaccata ctggaatccg aggttcgaat cgaaagtaac tgccggtagg
    16441 gcgcccccaa acttccagcc ggttttaact ttcttctcct ggcctgaaag tgaaaataca
    16501 gttaaaagac aaagcaacag ggaaaagata tttttcatgg tatacaggtt tggttaacag
    16561 ggttaaagat aaagaaaaat gaaaaccgca tgtatatcat atacaaacat gcagaaattt
    16621 tattttacgc tcaggctccc tgtacgggga gactagcagc tgccatttca atcaaatcat
    16681 aaccctgtta tgatggaaat tccggatact ttttcaatat ttgctaactt ggggaactga
    16741 taaatgattt tatatcagca tggcacttct gatcacaaat atcaagggtc ttgtcggagc
    16801 cggcgccatt cagcgcagcc tggtggcagg taaggaaatg tcggagctga ccacaattcc
    16861 cgatgcttac atattttgca gggacggcct gataactgat ttcggacaga tgcgggatat
    16921 ggatcctgca gttgcggaag aatccggacg tgaattgatt accattgatg cttcaggaaa
    16981 atttgttttt ccttcctttt gtgattccca tactcatctg gtttacgcag ggagccgtga
    17041 acaggagttt gccgacagaa taagaggctt atcatatgag gagattgcca ggcgtggcgg
    17101 cgggatcctc aattcggcaa ggttgctgca cgccacttcc gaagatgaac tttatgaaca
    17161 atcgtccgaa agaatacggg agatcatacg acagggtacc ggagccgtgg aaataaaaag
    17221 cggctatggt ttgaatacag aagatgagat aaaaatgctg aaggtaatca ggagattaag
    17281 ggacgatttt cccattgagg tccgatccac ctttctcggg gctcatgcac ttccggatga
    17341 attcaggtct gacagggaag cttacataga tcttgtagtg aatgaaatga tcccgatggt
    17401 cgcttacggg gaactggccg attacatcga tgttttctgc gatactggtt tcttcacggt
    17461 tgaggaaacc gaaaggattc ttatggccgg gataaaatac ggtatgatcc cgaagataca
    17521 tgccaatgag ctggattatt ccggtggtgt ccagacaggt gtcaggtatg atgccctttc
    17581 tgtggatcat ctcgaaagga cgggcgacgc tgaaatagaa gcccttcttg gtacgggaac
    17641 aatgcccacg cttcttcccg gatcggcact gttccttggt cttcctgatc ctcccgcacg
    17701 caggatgatc gatgcagggc ttccgttggc acttgcctct gatttcaatc ccggatcatc
    17761 accatccggc aatatgaagc ttgtcatgtc ccttgcctgt atcagattaa aaatgacgcc
    17821 cgaggaaacg atcaatgccg ttaccctgaa ttctgcctat gccatgggcc tctctgaaac
    17881 gcatggctcg atagcaatgg gaaaagcagc caatatgttt gttaccaggg aaatgccttc
    17941 gtacggattc tttccttacg ctttcggaag cgatttgatt gagacggtta tactgaaagg
    18001 acagattatt ccatgaacca gggaccctgg tgaactttcg ggaaccccgg aataagttct
    18061 tgtatttcat gtgatccgga gtaattgcag acaatattaa caggccaaat cagataatat
    18121 ccttaataat gcgaattaag agttaaaaat taagtaaatg aaagctgctg cgatttttcc
    18181 aaagacatgc acgagcaaac ctttggtaga tcgcacttta gacgctcttt gaatatctgt
    18241 tttgtctata agccagttga
//
LOCUS       DAOOYG010000046        18241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2945_length_18241_cov_7.911855, whole genome shotgun sequence.
ACCESSION   DAOOYG010000046 DAOOYG010000000
VERSION     DAOOYG010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18241
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2945_length_18241_cov_7.911855"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..2246
                     /locus_tag="PK288_05795"
     CDS             270..2246
                     /locus_tag="PK288_05795"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPM18466.1"
                     /translation="MKYKKNLKHLSLLVFSSLFIISTVSAQIRAFAREDFADPPVRYW
                     PRPLYFWNNTTIKPEVLTGQMQKMRDLCGYGGFGVVPFGRHFKPEYLSEEYLQLYGIM
                     LEKAKELGMTISLYDEFGFPSGSAGAFDEGDGIPRFQMKYPGYTIQRLDKYEEEVSGP
                     LLYERKVPDGKTMGIVAMKTEDRQRVDLSSSVTNGSLVWNVPAGRWKIMFFTCVIDGN
                     PLADYLNPEAVRKFTGMVHDVYYDRFRDYFGNVIYGTFFDEPSMFRAQFRMWTPMFNE
                     KFIAKYGFSPVTLYPALWYDIGPETSSARNYLFGFRAELFSEGYPKVVNDWSVAHGIT
                     ATGHTAPEEAIIPANGPGDLMKVFRHLEIPGIDKIGGHRYAERFYKLVSSAAYNWDRG
                     LVMSETYGAMPNYDEPGDLNWNDIWAIANDQYSKGINMLIPHAFWYDNTKVTYKPELS
                     YRNPLYADSLKAFTTYLARLNVILQAGGRHVADVAILYPIHSLLGDHYFYTETGPANV
                     DGPVGLDNSFYNEAISQIDYISFDGWLLNGIGIDYTYIHPEVLDEKCVVEKGKLHLRN
                     KVNHESFSILIVPSCYMISTSNLRKIVDFYNSGGMVVFTTRLPRKACEPGKDNEILSM
                     VQSIFPLMEENEHELLENERGGKACFFSTPMHWI"
     gene            2213..2548
                     /locus_tag="PK288_05800"
     CDS             2213..2548
                     /locus_tag="PK288_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18467.1"
                     /translation="MFLQHPDALDIAGTLSGSGLVFDVDHPLNPGLQYIHKVVEGKNV
                     FFFANTGEAPVQTEATLRDAVHLEEWDPHSGTIREADYRFAENASGSESTVVTINLKP
                     YNSVFFIEK"
     gene            complement(2608..3882)
                     /locus_tag="PK288_05805"
     CDS             complement(2608..3882)
                     /locus_tag="PK288_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPM18468.1"
                     /translation="MKRELNATVTLVMYLLTVSLAAGQNIGLVDVKRAEQVISVGGSG
                     ADIPGYSSSAIQIALDALKTRGGGTVRLNPGTYEITGPVRLSDNVSLIGSGETTILRK
                     CDGFRTSFIIDADWGMVKAVVKDAGGFSKGMGIQLFDDDHKQAWAVTTALITDIDGNV
                     IYFDNNTVHDYVASKNGTVTNACSVVEAVEVKNVRIADLMIEGNKDRNDYINGCRGGG
                     VYIHKSRNCLVENVRVNEFNGDSFSWQITENITLKGCEASNGNGLGFHPGTGSDHSVM
                     ENCSGHHNAGDGLFLCWRVQNGIFRDNVFFANGDNGISIGHKDTDNIFENNHVYENAH
                     QGVLFRDENEQNAGHRNTFTGNIIENNGMKGEANGFYISGVTRDIVIKNNIIRSTGKG
                     NQKTAIRVGKNSSAITASGNTVSGSKEIIYEK"
     gene            complement(4064..7306)
                     /locus_tag="PK288_05810"
     CDS             complement(4064..7306)
                     /locus_tag="PK288_05810"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HPM18469.1"
                     /translation="MSKLTALRNRALQVLLLISPALVFPDLTGQDVVVKEELKPFKTY
                     PFSDPNPVSVRALNSMASSFYPYFVFDGFTEKPEVKMWKVITLENDFISVTILPEVGG
                     KVWGAVEKSTGKEFVYTNKVMKFRSIGIRGPWTSGGIEHNFGLDLGHAPWTSSPVDYV
                     IKEHPDGSASCIVGGLDLASRTQWRVEIKLDKDKAFFETNSFWYNPRPLHDAYLSWEN
                     GGFRASDDLQFFFPGTCYIGHDGNVDQWPVDREGHNLSYYRENNFGSSKSYHVSGLFT
                     DWFGGYWHNSGFGFGHWAPYSDAPGKKIWIWSLARDGAIWEKLLTDNDGQYIEAQSGV
                     KFNQASNVSGFNSPFDQLSIKPYYTETKTESWFPVIGTGGMVDASPAGTLNVVTAGDS
                     LKILISPNIFLKDTLVIRNNGKIIHSACIELKPMKLYSGSVALNIADIGDLNVTLGKN
                     ILEYNSGTETLKTERPSRSPVNGDYNSAEHLFLLAEDMYAMREFTRALNYYYKCLEKE
                     PSHLRALYKAAETEFRMYRYRNAEDLAKKILEINTYDPGGNFITGLINRRRGMINQAE
                     EAFSIAARTMEFRSASFIQIAEARLLRGDFRGADDYAGKALDYNRISVPAREILIISS
                     RKLHNNAKAEKQIGELLDIDPLNHFANFEKYLLSPSTARLNDFKSLIRNELPHETFLE
                     LALDYVNRGLYDEAAAVLGEAPANPIVFYWLAWLRKDTDVALSTQLLRKAADLSPFLV
                     FPFRHETISVLEWALEKNDSWKTRYYLGLIYWHIGRLEKTLELFRECGYAPDYAPFYI
                     ARGILSQSAGRNSPPLGNDFSKALELDANDWRTWHFLAENLQSEKSYNEQLVKARQAY
                     LRFPSNPVTGIDYVKALINNARYSESLKILETITILPYEGAREGHELYELANLSLAVS
                     LIEQGKYDKAIKFIDNSKKWPENLGAGKPYDPDERFQDYLLSFCYKMKGKNKMTDYYS
                     NRIIRYSGNDIPGEDPVNRYISTIAYNKAGKRQEAVIAMENWKAGQDSLSNWSISPGS
                     RSPKVQWVIAKFNGNNEVSDRLEKEISDMPAEAMFRLFLRSYDIIDPKKK"
     gene            complement(7606..9546)
                     /locus_tag="PK288_05815"
     CDS             complement(7606..9546)
                     /locus_tag="PK288_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPM18470.1"
                     /translation="MRTSFVVLLIICSFCSCSSPRNDFSGSGWTVSVIPSSVRLDPTS
                     NEIIDNRFVLLKGNKDVNSDILKKNSIYDGNTVSLHAARGEYISFQLVLTSLTKETLD
                     NIRVGMIPFRKGEDEFSIKPELFLEWAVEVKTPSTGYPVASLGNGWYPDALIPFQYIQ
                     DDSSRVRWRWTYPLWLPDFNNRIDDQKSLVIWIDQYIPFLRNEAAPGTYSSELAITIK
                     GLTRKIPVSLTVWDFALPNENRFRAGLQHEGFTRNMTGRNELEIYQLLKRNRISMLDP
                     TYEPELEVRSNGNIVIDWKALDERLDKYFTGKAFTAEYGYNYGPGYGEPIETFVLPFD
                     VAGKHGTKGWPDIGGPGVERNPSNRAIYTECIRKVRDHYKNIIDPDKTGITVYLNGLD
                     ESYFPEAWSRMVYYGDIFRELYPEADFRIDGGYSEEAMAIVKNSISAWASHTIEYDYE
                     TVKKYQDMGIKVWLYGPLLYESKVNSWVGSSTFLDLPLVNDRAISWSCWKYRTYSWLS
                     WGAGAGWVNGWYDPESWKDASKARPESEAEFTYKKINGNALLIYAAGVVPNVNGPCPS
                     IRLKAMRDGVQEYEYLRLLSEADGNTHRADSIVNEIIHEPFGPKSIGNLDVWSFDAGK
                     WDAQRIAMGRMIERKLQDRNKK"
     gene            complement(9575..10720)
                     /locus_tag="PK288_05820"
     CDS             complement(9575..10720)
                     /locus_tag="PK288_05820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM18471.1"
                     /translation="MVSRREFIKLTSMGAAVISTAGMNQCMNTGSSGKLKPAGGRLID
                     AHIHATPGKIEKALRVMDDNLIRYAVLIASISGSDPDYYVGDRAFYEINDAIKPYRNR
                     LGLHYTYDWSLAENDPEFFIKAPDMLEKAVDAGAIALKNLKDLGLTARDREGKLIPID
                     DPRLFPIWERAEKLGIPVAFHTGDPVAFFQPWNPENERWEELELHPEWSFADRSKYPA
                     LETLFDQVNNVYRTFPGVKFVAVHVAGYSENLKAVSSWLDQLPNLSIDTAARIGELGR
                     HSSGEGHEFFTKYQDRIMFGTDLAFWNECDVQGAGPCKDFTMEENHHFYDIHWRYFQT
                     LDKQFDHPTPIQGNWKIDGIGLDESVLKKLYWDNAFRFYKLDRFGVA"
     gene            complement(11132..11983)
                     /locus_tag="PK288_05825"
     CDS             complement(11132..11983)
                     /locus_tag="PK288_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPM18472.1"
                     /translation="MSSKIQIARLSVLSNSFLITIKLAAGVISGSVSIISEAIHSAMD
                     LIASLIALFSVKVSDNPPDSRHPYGHGKVENVSGVIEGILILAAAVWIIAESVKKMAG
                     GSMVVESLGLGSLVMLVSAIINSIVSGKLYRVAKATGSVALEADALHLKTDVYTSLGV
                     AAGLGLIILTGIHWFDPLIAICIALLIIREAVILIKKAFRPLLDEAWPDDEIKKLEMM
                     LNSMDVNYHDLRTRISGNYRFIDFHIGIPKDKSVEDAHTYCDSIEETLKSKFDKLNIT
                     IHVEPEP"
     gene            complement(12226..13101)
                     /locus_tag="PK288_05830"
     CDS             complement(12226..13101)
                     /locus_tag="PK288_05830"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPM18473.1"
                     /translation="MRVEIDNRSGFCFGVQNAVKIAENALLNGERVFSLGPIVHNDKE
                     VERLSDLGLISIDHNQFSELRNCKVLIRAHGEPPETYEKAERNNIEIIEATCPIVKKV
                     QSRIRDAWISSKADNGQVVIYGKSGHAEVIGLLGQTNNEGILISSVDDLCKIDFTRPV
                     HLFSQTTMDAKEYRKIKELIAGNMKAAASGSQEISFKIHNTVCGQVSNREPHLKEFAG
                     NHDIIIFVSGRESSNGKMLFNVCKSVNPDTYFVSSVDEIESSWFSNKKFAGVCGATST
                     PKWLIEQIRDHISKL"
     gene            complement(13091..13783)
                     /gene="cmk"
                     /locus_tag="PK288_05835"
     CDS             complement(13091..13783)
                     /gene="cmk"
                     /locus_tag="PK288_05835"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004377257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPM18474.1"
                     /translation="MKKIIIAIDGYSSSGKSSFARAIAREMNYIYIDSGAMYRAVTLY
                     CLRKGYVGNDFLKTADIISDLREINIGFAYNPDISGYETFLNSENVETQIRGVEVSSY
                     VSRISLIPEVRSRMVELQREIGRERGIVMDGRDIGTVVFPDAELKIFMTASVEIRAKR
                     RYDELKGKNMDVDFETIKNNIIVRDITDENRDISPLRRADDAIVLDNSRMTITEQLRW
                     IREIIKNKLNEG"
     gene            complement(13830..14870)
                     /gene="porQ"
                     /locus_tag="PK288_05840"
     CDS             complement(13830..14870)
                     /gene="porQ"
                     /locus_tag="PK288_05840"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HPM18475.1"
                     /translation="MKKVLFIAALSILLPELFPQTGGTRVYEFLNLTHSGLVASLGGS
                     NVSMPAGDLNLAHHNPSLLSPAMDKNLSLNYASYLAGINYGMVLYSRSTANAGNFAGG
                     VTYLNYGTFTEADPSGNITGSFTASEYSFSLICSYSIDSSFSVGIDLKPILSHLERYT
                     SAGFAADIGVSWKSDDGLYSGGIVLRNMGRQIISYTGEKNRSTPFEILAGASAKLAHA
                     PFRFSLTIRHLEKYDLTHDYKDPAGNGAKGSPDGLMENIMRHMIMGAELIPHKNFYLS
                     AGYNYQRRKELMTESKAAGAGFSWGFGINTSFMSLQFGHASYHLAGASNHISLITRTD
                     QLYRKFRRQNKN"
     gene            15067..16878
                     /locus_tag="PK288_05845"
     CDS             15067..16878
                     /locus_tag="PK288_05845"
                     /inference="COORDINATES: protein motif:HMM:NF023677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/N-terminal domain-containing
                     protein"
                     /protein_id="HPM18476.1"
                     /translation="MNRVRHWQSCCSVCWSRFFISVANGTGFDNYYFSNTVNNNCNFF
                     VCDVDGDGYSDLISTDKDSPWTTGYQVFRPGGNSTALMEKVGNGLGELTKVSYLKLSK
                     ANSSSYQRGSSAVFPLTDFQGPLTVVSSFQTDNGKGSMNLMEYYYEGAKIHLRGKGFL
                     GFLKTRLTDHTAGIANEAIITDGFNPTYFYPSVQLSLSKRAGTTDTISKVKNVLSQVV
                     LNASKKWIFPYVSSSKVTNKLTGHTVTMNTSGYDNYGNPSTIVKSYSSGPTETSTIVY
                     DNTVSTSQWLLGRPTSATTQYTGGGNTITRAVTRSFSTTTNNLNSETWYPGTGNQFVK
                     GYKYNSNGTLKRDSVTVGGVYRTNIYTYESNGVRIKTITDPLSHTATITYDACGRLQA
                     VKDYLNNTLSYQYDEMGRVTNVSSTDGNQTSSVYAWESPASAPKPARYSVVVTGNDGS
                     QTKRWFDKLSREIRSDMKGFDGSWIYTDTRYNVKGQVDSISEPYYTGPTWNRYQYDNY
                     GRKTNLYRPSGRNSSWVYSSNTITETTGGRSFSKTYSSDGTVSSATDAGGTISYTYYP
                     DGKVKNITAPGNIVTQMQYDIAGNQTKLIDPSAGTIN"
     gene            16899..>18241
                     /locus_tag="PK288_05850"
     CDS             16899..>18241
                     /locus_tag="PK288_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPM18477.1"
                     /translation="MQTQQNSRLQTTTITYYPDGRIARKVTPEGTTKYWYNANKQLTN
                     IGSPGGVSRSFAYDSKGRPATITDTIPGTTPLIKTLTYDGYGRPGTITHPSNIVETRN
                     YNSNGYLSSVSAGGSARWTVTSMNALQQITGGRYGTSLNAAYGYDSYGYLTSNTTGSL
                     QNYTYSFNTVTGNLNWRKNVLQANIQENFEYDNPDRLDRVYRGGTTLLDMAYESNKGG
                     ITTKSDAGTLNYNISAKPYALGTVNPSAGLLPTTVDSLTYTSFEKVNTISEGNFAAYF
                     TYNSDNERAKMEVKQNGSTILTRWYPSGGYIKETAGGATKEYTFIGGDAYSAPVVAIT
                     QGGTTTYYNLLRDHLGSITHVVNASNNTLLYEYSYDAWGRMRNVSSWTSYAPGSEPSL
                     FIAGRGFTGHEHLPWFNLINMNGRVYDPLVGQFLSPDNYVQAPYFTQGLNRYVYCL"
BASE COUNT         4703 a         4255 c         4173 g         5110 t
ORIGIN      
        1 ctctcggcgg ggctgaacac ggcaacaggg tacttgaaac cagcagggag agtgttctgg
       61 cagccattaa aaaggacctg gagaccctaa aaggttttct tagagaagca gggctggctt
      121 aatgctaatt aacaatgcag ttaagggcgc gttcaaagat ccggatcagg acaattgcat
      181 gtaactgcct tgtatccctg agcctcagta ttaaaacaca aactgctttc ttgatgtaat
      241 ttctgaatct aaatccatca atacaggata tgaaatacaa aaaaaatctg aaacatttgt
      301 cgctgcttgt tttttccagt ctgttcataa taagtaccgt ttcggcgcaa atcagggcat
      361 ttgccaggga ggattttgcc gatcctcctg tccggtactg gccgagacca ttgtatttct
      421 ggaacaatac aacaataaaa ccggaagtac tgaccgggca gatgcagaaa atgcgtgatt
      481 tgtgcggtta tggaggattt ggcgtggtac ctttcggcag gcatttcaaa cccgaatatc
      541 tttctgaaga gtatctacag ctatatggga taatgcttga aaaggcaaag gaactcggaa
      601 tgacaatcag tctgtatgac gagttcggat ttccaagcgg atcggcgggt gctttcgatg
      661 agggtgacgg aatcccgcgc tttcagatga agtatcccgg ttatacaatt caacggctgg
      721 ataaatatga agaagaagta agcgggccgc ttctgtatga aaggaaggtt ccggatggga
      781 aaacaatggg catagtggca atgaaaactg aagacaggca aagggttgat ctgtcatcat
      841 ctgtgaccaa tggttcgctg gtatggaatg ttcccgccgg aaggtggaag attatgttct
      901 tcacctgcgt gattgacggc aatccccttg ccgattacct taatcccgag gctgtcagga
      961 aatttaccgg catggtccat gacgtttact atgaccgttt cagggattat ttcggcaatg
     1021 tgatctacgg gacatttttt gacgaacctt caatgttccg ggcacagttc aggatgtgga
     1081 caccaatgtt caatgagaaa ttcatcgcga aatatggctt cagtcctgtt accctgtatc
     1141 ctgcactgtg gtacgatata gggcccgaaa cgtcatctgc acgcaattat ctgtttggtt
     1201 tccgggccga actgttttcg gaaggatatc ccaaggtggt aaatgactgg tcggtggcac
     1261 atggtataac agccaccggt catacggccc ccgaagaagc aattatccct gccaatggcc
     1321 ccggcgacct tatgaaagta ttcaggcatc ttgagatccc gggaattgac aagatcggcg
     1381 gacaccgcta tgcggaaaga ttctataaac tcgtcagttc ggctgcatat aactgggaca
     1441 ggggactggt tatgtccgag acctatggtg cgatgccgaa ttatgatgag cccggcgacc
     1501 tgaactggaa cgatatatgg gccatagcaa atgatcagta ttcgaagggg ataaacatgc
     1561 ttatccccca tgcattctgg tacgataaca ccaaggttac ctacaagcct gaactgtcat
     1621 accggaaccc gctctatgcc gacagcctca aagctttcac aacctatctt gcaaggctga
     1681 acgtcatact ccaggccggc ggaaggcatg tggcagatgt ggcaattctt tatcccatcc
     1741 attccctcct tggagatcat tatttctaca ctgaaacagg ccctgcaaat gttgacggac
     1801 cggtcggact tgataacagc ttctacaacg aggcaatttc tcagattgac tatatatctt
     1861 tcgatggctg gctgctgaac ggtattggaa ttgattatac ctacattcac cccgaggtcc
     1921 tcgatgaaaa atgcgttgtg gaaaaaggaa agcttcacct caggaataag gtcaatcatg
     1981 aaagtttcag catactcatt gttccgtcat gttatatgat ttcaaccagc aacctgagga
     2041 aaatagttga tttctacaat tcaggaggga tggtggtgtt caccacccgc cttccgcgta
     2101 aagcttgtga accgggaaag gataatgaaa tattaagcat ggtccagtcg atttttccgt
     2161 tgatggagga aaatgaacat gagcttttgg agaatgaaag gggaggaaag gcatgtttct
     2221 tcagcacccc gatgcactgg atatagccgg gacattatcc ggatcaggcc tggtgtttga
     2281 tgttgatcac ccgctgaacc ccggtttgca atatattcac aaggttgtgg aaggaaagaa
     2341 tgtttttttc tttgccaaca ccggcgaggc accggttcag actgaagcaa ccctcaggga
     2401 tgcggttcac ctcgaagaat gggatccgca ttccggcacg ataagagagg cagattaccg
     2461 gtttgcagaa aatgcttcag gttctgaatc aaccgtggtg acaataaacc tgaagccgta
     2521 taattcagta ttttttattg agaaatagga gacagggaat tttcaggtaa agttaccccg
     2581 gctgttgatt ccggggatcc tgctttccta tttttcgtaa atgatttcct ttgatcccga
     2641 aacagtgttt cccgatgcgg ttatggccga gctgttttta cccacacgta tggcagtttt
     2701 ctgatttccc tttcccgttg accttattat attgtttttt atcacaatat cacgtgtgac
     2761 gccgcttata taaaagccgt ttgcctcccc cttcatgccg ttgttttcaa taatgtttcc
     2821 ggtgaacgtg ttgcggtgtc cggcattctg ttcattttca tcacggaaaa ggacaccttg
     2881 atgagcattt tcgtacacgt ggttattttc aaaaatgttg tcggtatcct tgtgtccgat
     2941 tgagatgccg ttatcaccgt tggcgaaaaa aacattatcc ctgaatattc cgttttgcac
     3001 cctccagcag aggaacaatc cgtcgcctgc gttatgatgt ccgctgcaat tttccattac
     3061 cgaatgatcg gagccggtcc cgggatgaaa tccgagcccg ttcccgtttg aggcctcgca
     3121 tcctttaagt gtaatgttct cggttatctg ccagctgaaa gagtcgccgt taaactcatt
     3181 gaccctgacg ttttccacca ggcaattcct tgatttgtgg atataaactc cgccgcccct
     3241 gcatccattt atataatcat tcctgtcctt gttcccctca atcatcaggt ctgcaatcct
     3301 tacatttttt acctccacgg cttcaacgac ggaacaggca tttgtcacag ttccgttttt
     3361 tgatgccacg taatcatgta cggtgttgtt gtcaaaatag atcacgtttc cgtcaatgtc
     3421 tgtaatcaat gccgttgtta cggcccatgc ctgcttatgg tcgtcgtcaa aaagctggat
     3481 tcccattcct ttactgaacc ctccggcatc ttttacgact gcctttacca tgccccagtc
     3541 agcatcaatg ataaaactgg ttctgaagcc gtcacatttc ctgagaatgg tagtctcacc
     3601 cgatccgatc agggaaacat tatcggaaag gcgtacgggt cccgttatct cgtatgtgcc
     3661 cgggtttaat cttacggttc ctccgcccct ggtttttagc gcatccaggg ctatctggat
     3721 tgctgatgaa gaataacccg gaatatcggc tccggatcct ccgacgctta taacctgttc
     3781 tgccctcttt acatcaacca ggcctatatt ctgcccggct gcaagggaaa ctgtaagcag
     3841 gtacatgacc aatgttaccg ttgcgttcaa ttctcttttc atttttttgt ctgttaaatt
     3901 attaacaatt caagttcaat ttcggctctt taattttcgc aagcgggaag gaaacaatgg
     3961 ttgttgaaat aagggcagtc aacctgtttc ccgcctgcct tgcttctgga gtatcttcat
     4021 ttttgcctgt ttattcaggt cagataattt caaaataaat gaattatttc tttttaggat
     4081 caataatgtc ataagatctg agaaacaatc tgaacatggc ttcagccggc atatctgata
     4141 tttctttctc caggcggtcc gatacttcat tgttgccgtt gaattttgca atcacccact
     4201 gaactttagg tgatcgtgat ccgggtgaga tactccagtt gctcagggag tcctgcccgg
     4261 ctttccagtt ttccattgcg atgacggctt cctgcctttt gccggctttg ttgtatgcaa
     4321 tggttgaaat atacctgtta accggatctt caccaggaat gtcgtttccc gaataccgga
     4381 ttatccggtt tgaatagtaa tctgtcatct tgttttttcc tttcattttg tagcaaaacg
     4441 acagcaggta atcctggaag cgctcatcag ggtcataagg tttaccggca cccagattct
     4501 ccggccattt ttttgagttg tctatgaatt tgattgcctt gtcatatttc ccttgttcaa
     4561 tcaggctgac tgcaagtgaa agatttgcaa gctcgtaaag ctcatgaccc tcccgggccc
     4621 cttcataggg cagaatggtt attgtctcca atatcttaag gctctctgaa tacctggcgt
     4681 tatttatcag ggcttttaca tagtcaatcc cggtcacagg gtttgaagga aaacggagat
     4741 atgcctgcct ggcttttacc agctgttcat tgtatgactt ttccgactgc aggttctccg
     4801 cgaggaaatg ccatgttctc caatcattgg catcaagttc cagggctttc gaaaaatcat
     4861 ttcccagggg cggggagttc ctgccggctg attgtgacaa aatacctctt gcaatgtaga
     4921 acggtgcata atccggggca tacccgcatt ccctgaacaa ttccagggtt ttctccagtc
     4981 tcccgatatg ccagtatatc aaaccaagat aatacctggt tttccagctg tcgttttttt
     5041 caagggccca ttcgagaaca cttattgttt catgccggaa tgggaaaacg agaaaggggg
     5101 agaggtctgc ggccttcctt agcagttgtg tgctcagggc gacgtcggtg tctttcctga
     5161 gccaggcaag ccagtaaaaa acaatcgggt tggcaggagc ctccccaagc acggctgcag
     5221 cttcatcata caatcccctg ttcacgtaat ccaaagcaag ctccaggaag gtttcatgag
     5281 gcagttcatt ccggatcagt gatttgaaat catttagcct ggcagtggaa ggagagagaa
     5341 gatatttttc aaagttggca aaatggttta aagggtcaat atcgagcagc tctccgatct
     5401 gtttttccgc tttggcattg ttatgcagtt ttctgctgct gatgatgagg atttcacgtg
     5461 ccggaacaga tatcctgtta tagtccaggg cttttcctgc ataatcatct gcacccctga
     5521 agtcgcccct cagcagcctt gcttccgcaa tctggataaa tgaagctgac ctgaattcca
     5581 ttgtcctggc ggcaatactg aaagcctcct ctgcctggtt gatcattccg cgtcttctgt
     5641 tgatgagccc ggtaatgaaa ttaccccccg gatcataagt gtttatttca aggatctttt
     5701 ttgccagatc ctcagcattc cggtatctgt acattctgaa ttccgtttcg gctgccttgt
     5761 aaagggcccg caggtgtgaa ggctcctttt cgaggcattt atagtagtaa ttcaaagccc
     5821 tggtaaactc cctcattgca tacatatctt ctgccaggag gaacagatgt tcggctgaat
     5881 tatagtcacc attaaccggc gatcttgaag gtctttcagt ttttaaggtt tcggtcccgg
     5941 agttatactc caggatgttt ttacccaaag taacattcag atccccgata tccgcaatgt
     6001 tcagggccac tgacccggaa tacagtttca ttggcttcag ttcaatgcat gcggaatgaa
     6061 tgattttccc gttgttcctg atgaccaggg tgtcttttag gaagatattc ggacttataa
     6121 ggatcttcag gctgtcgccg gcggtcacca cgttgagggt gcctgccggg gaagcatcga
     6181 ccatccctcc ggttccgatt accgggaacc atgattctgt tttggtttca gtatagtagg
     6241 gttttattga aagctggtcg aacggactgt tgaatccgct cacattgctt gcctggttga
     6301 atttaactcc cgactgggct tcaatatact ggccgtcatt gtctgtcagc agtttttccc
     6361 agatagcgcc gtcgcgcgcc agcgaccata tccatatctt tttgcctggt gcatcagagt
     6421 agggggccca gtggccaaat ccgaagccgg agttatgcca gtatcctcca aaccagtcag
     6481 tgaacaggcc ggatacatga tacgatttgc tgctcccgaa attattttcc ctgtaatatg
     6541 aaaggttgtg tccttccctg tcaaccggcc attggtcgac atttccgtcg tggcctatgt
     6601 aacaggtacc cggaaagaag aactggaggt cgtcggaagc gcgaaacccg ccattttccc
     6661 acgacagata cgcatcgtga agggggcggg gattatacca gaagctgttt gtttcgaaaa
     6721 aagctttatc cttatccagt ttgatttcaa cgcgccattg tgtacgtgat gccaggtcaa
     6781 ggccacccac gatacagctt gcactgccgt cgggatgctc ctttattacg taatcaaccg
     6841 gggaggaggt ccagggagca tgacccaggt caagaccgaa attatgctca atcccgccgc
     6901 tggtccatgg acccctgata ccaattgacc tgaatttcat gactttattc gtgtaaacaa
     6961 attcctttcc ggtcgatttt tcaacagcac cccacacctt gccgccaact tccgggagta
     7021 ttgtgacgct gataaaatca ttttcaaggg tgattacttt ccacattttc acctcgggct
     7081 tttcagtgaa accatcaaaa acaaagtagg gatagaaaga ggaggccatt gaattcaatg
     7141 cccttaccga cactgggttc ggatcggaaa aaggatacgt tttaaatggt tttaattctt
     7201 ctttgacaac aacatcctgg ccggtcagat ccgggaaaac cagagcaggc gaaatcagta
     7261 ataatacttg cagggccctg ttcctcaggg ctgttaactt agacatggca aaattggttt
     7321 tatagaatca gatttaatga taacaaggtt agcggataac tggttttatt ttttcatgga
     7381 cggcttgcac ctaatctgtc atttcctctg aagctgcgat agttattcgg acttttccaa
     7441 tcggtattga accagccgga ttgtctttcc gggtcttcct ggtaacggga tcaacatggt
     7501 tattggccaa taatttatct ttccgtcaaa gccggccatt aatctccctg gcagatccca
     7561 tcatgttttc cggattgcac agcgttcagg gatccgggtt tgtgattact ttttattcct
     7621 gtcctgcaat ttcctttcaa tcattctgcc catggcgatt ctctgggcat cccattttcc
     7681 ggcatcgaag ctccagacat cgagattccc gatggatttt ggaccgaaag gttcatgaat
     7741 tatttcgttt acaattgaat cagccctgtg agtgtttccg tctgcctccg acagcagtct
     7801 caggtactcg tattcctgca ctccgtcgcg catggccttc agcctgatgg acgggcaggg
     7861 tccgtttaca tttggaacaa cgccggcggc ataaatcagg agagcattgc cattgatttt
     7921 tttgtaggtg aattcagcct cagattccgg tctggctttt gaagcatcct tccacgattc
     7981 aggatcgtac catccgttta cccatccggc tccggcaccc cagctcagcc acgaatacgt
     8041 gcggtatttc cagcagctcc agcttatggc cctgtcgttt acaagcggca ggtcgaggaa
     8101 ggtcgaactg ccaacccagc tgtttacctt gctttcataa agaagcggcc cgtaaagcca
     8161 aacctttatg cccatatcct ggtatttttt cactgtttca taatcatatt caatggtatg
     8221 ggaggcccat gcactgattg aattcttcac gatcgccatg gcttcttcac tatagccgcc
     8281 atcgatgcga aaatcggctt cgggatacag ttcacggaaa atatcgccgt agtaaaccat
     8341 tctgctccag gcctccggga aataggattc atccagtccg ttgagataga cggtgatacc
     8401 agttttgtcg ggatcgataa tatttttata atgatcgcgg actttcctga tgcattcggt
     8461 atagattgcc ctgtttgaag gattgcgttc aacgccgggt cctccgatgt cgggccagcc
     8521 ctttgtccca tgctttccgg caacgtcgaa aggcagtaca aaggtctcta tcggctcacc
     8581 atagcccgga ccgtaattgt aaccatactc ggctgtaaat gccttaccgg tgaaatactt
     8641 gtcaagtcgt tcgtcaaggg ctttccagtc aattacaata ttcccgttgc ttctgacctc
     8701 cagctccggt tcataggtgg gatcaagcat tgaaatcctg ttgcgtttaa ggagctggta
     8761 tatttcaagc tcatttctcc cggtcatgtt tctcgtaaaa ccctcatgct gaagtcccgc
     8821 cctgaacctg ttctcattgg gaagagcgaa atcccatacg gtgagactga cgggtatctt
     8881 cctggttagt ccttttattg ttatagccag ttccgacgaa taggtaccgg gggccgcttc
     8941 attcctgaga aaggggatgt actgatcgat ccatatcacc agtgacttct gatcatcaat
     9001 gcggttgtta aagtcaggaa gccacagcgg gtatgtccac cgccatctta ccctcgatga
     9061 atcgtcctgg atatattgga acggtatcaa tgcatcggga taccagccat tgcccagtga
     9121 ggcaacagga tatccggtgc tgggtgtttt gacttcaacg gcccattcga ggaaaagctc
     9181 cggcttaatt gaaaactcat cttctccctt cctgaacgga atcatcccaa ccctgatgtt
     9241 gtcgagggtc tcttttgtaa gactggttaa caccagctga aatgagatat attctcccct
     9301 ggcggcatgc aatgacaccg tgttaccgtc atagattgaa ttcttcttca ggatatctga
     9361 attgacatcc ttattccctt tcaaaagaac aaaccggtta tcaatgattt cattgcttgt
     9421 cggatcgagt cttaccgatg aagggatgac cgataccgtc cagcctgatc cgctaaaatc
     9481 attcctgggc gatgaacagc tgcagaagga gcaaataatc agtaaaacaa caaaagaagt
     9541 tctcatagtg ccggtattta gtttatggtt agattcatgc aacaccaaac ctgtcgagtt
     9601 tatagaacct gaatgcatta tcccagtaga gttttttcag aacactttca tcgagtccga
     9661 tgccatcgat tttccagttt ccctggatcg gcgtcggatg atcgaactgt ttgtcaagtg
     9721 tctggaaata acgccagtgt atatcgtaga aatgatggtt ctcctccatt gtgaaatcct
     9781 tgcatgggcc ggcgccctgg acatcgcatt cgttccagaa agccagatcg gtgccaaaca
     9841 ttatcctgtc ctggtatttg gtgaaaaact catgtccttc accggagctg tgccttccaa
     9901 gttctccgat gcgtgcggcg gtatcaatgc tgaggttggg cagctggtcg agccatgagc
     9961 tcactgcctt caggttttcc gagtatccgg caacatgtac cgcaacgaat ttaactcccg
    10021 ggaaggttct gtaaacgttg tttacctggt caaacagtgt ttcgagggcc ggatatttac
    10081 tgcgatcggc aaatgaccat tcgggatgaa gttccagttc ttcccagcgc tcattctcag
    10141 ggttccatgg ctggaagaag gccaccggat cgccggtgtg aaaagccacg gggatgccaa
    10201 gtttttcagc tctttcccag atcgggaaaa gcctgggatc gtcgataggg atgagttttc
    10261 cctccctgtc ccttgctgtc aggcccaggt ccttgagatt cttcagggca atggcacctg
    10321 catcaactgc cttttccagc atatccggag cctttataaa gaattcaggg tcgttttctg
    10381 ccaggctcca gtcgtatgta taatgaagcc ccagcctgtt cctgtacggt ttaatcgcat
    10441 cattgatctc atagaatgcc ctatcgccta cataataatc cgggtctgat cctgatatgg
    10501 aagcgataag tacggcatac cttatcaggt tatcatccat aaccctgagc gctttttcaa
    10561 ttttgcccgg tgtggcatgg atgtgggcat cgatcaggcg gccaccggcc ggctttagtt
    10621 tcccggatga tccggtattc atgcattggt tcatacctgc tgtcgaaatt actgctgctc
    10681 ccattgatgt gagtttaata aattctcttc tgcttaccat ttttctcaat atttgttttt
    10741 aatgtgatca gtaaaatctc tatggccgtc ttaaggcccg gcttcttctt accttcctga
    10801 aattttattg cccggcctga atcatggcgg aattaagccc ataaccctgc cggatccggg
    10861 atagatgtct caaagcagat tttcccctgt attattgatg ttagaaacat attgttgact
    10921 aagataataa attcactgaa gaattgcgga cgggaaaaga atataatttc ccgtaacaga
    10981 tttccctgtg aggttgcttt cagtgattac ttgcggaata agggccgggt gcctgaaaaa
    11041 agctgcgggc aattgcggga accgggggag tgacgtaaaa tgagataatg agtaaacaaa
    11101 tatcatgaaa taaggaagct gaaaagattt gtcatggttc cggttccaca tgaatggtga
    11161 tattcagctt gtcaaatttt gatttcagtg tttcttctat actgtcgcaa taggtatggg
    11221 catcctcgac cgatttatcc tttgggatcc cgatgtgaaa atcgatgaac ctgtaatttc
    11281 ccgagatcct tgttctgagg tcgtggtaat tgacatccat gctgtttagc atcatttcaa
    11341 gcttttttat ttcatcatcg ggccatgctt cgtcaagcaa tggtctgaaa gcttttttga
    11401 tcaggataac agcttccctg atgataagaa gggcaatgca gattgcgatc agcggatcga
    11461 accagtgtat tccggtgagt attatcagac ccagtcctgc agccactccg agtgaggtat
    11521 aaacatcggt tttgagatgc aacgcgtcgg cttcaagggc aaccgatcca gtggcttttg
    11581 caaccctgta cagcttccct gatacaatgg agtttataat ggctgaaaca agcatcacca
    11641 gggagccaag tccgagggat tcaaccacca tactgccccc ggccattttt ttcaccgact
    11701 ctgcaatgat ccagaccgct gctgcaagta tcagtattcc ctcaatcacc cccgacacgt
    11761 tttccacctt cccgtgtccg tagggatgac gggaatcggg gggattgtct gaaaccttca
    11821 cggaaaacag cgctatcagg gatgcaatca ggtccatggc cgagtggatc gcctcggaaa
    11881 tgatactgac cgatccgctt atcactccgg ccgccagctt tattgtgata agaaaagaat
    11941 ttgacagtac tgacagcctt gctatctgga tttttgaact catccggggc cctgaaaaat
    12001 ttaattcctg cagataaaat taaatattat tcttaaataa tcagggctgt ggaatacaac
    12061 ccggggctca atggttcaga tgcaaccgga ataccgccca atgccggcag cctgcagttt
    12121 attgcggctg tctttttatt attgttaatt gggatagcgg ttaaaataac cgggccgtga
    12181 aacagattaa ctgccagggg agttcactaa ccgggtttta tgtgctcaca gttttgaaat
    12241 atggtcccgg atctgctcta tgagccactt gggtgttgaa gttgcgccgc agaccccggc
    12301 aaatttcttg ttgctgaacc atgatgattc tatttcatca accgaggaaa cgaaatatgt
    12361 atcaggattc acggatttgc agacgttaaa cagcatcttt ccgtttgagc tctcccggcc
    12421 gctgacaaaa attattatat catgatttcc ggcaaactct ttcaggtgcg gttccctgtt
    12481 tgagacctgt ccgcagaccg tgttgtgtat tttgaagctg atctcctggc tgcccgaagc
    12541 tgccgctttc atgtttccgg ctataagctc tttgattttc ctgtattcct ttgcgtccat
    12601 cgtggtttgt gaaaagagat ggaccggccg cgtgaagtcg attttgcaga gatcgtcaac
    12661 tgaggagatc aggatgcctt cattgtttgt ctgtccaagc aggcctatca cctctgcatg
    12721 acccgatttg ccgtaaatta ccacctgacc gttgtcagcc ttggaagaaa tccaggcgtc
    12781 cctgatcctt gactgcactt ttttgacaat gggacatgtt gcctcgatta tttcaatatt
    12841 gtttctttca gctttttcat atgtttcagg cggctctccg tgagccctga tcagtacctt
    12901 gcagttcctg agctcactga actgattatg gtcgatggaa atcaggccca ggtctgaaag
    12961 tctttcaact tccttgtcat tgtggactat cgggccaagt gaaaaaaccc tttcgccatt
    13021 cagcaatgca ttttctgcaa tcttaaccgc gttctgaacc ccgaagcaaa agcctgacct
    13081 gttgtcaatt tcaaccctca ttaagtttgt ttttgatgat ttcccgtatc catctcaatt
    13141 gttctgttat tgtcatcctg ctgttatcca gaacgatagc gtcgtcggcc cttcgcaggg
    13201 ggctgatatc cctgttttca tcagtaatgt cacggacgat gatattgttc tttattgttt
    13261 caaaatcaac atccatattt ttacccttaa gttcatcata tcttcttttg gccctgattt
    13321 caaccgatgc agtcatgaag atcttaagtt ccgcatcagg gaacactacg gttccgatat
    13381 ccctgccatc cattacaatt cccctctccc tgcctatttc cctctgaagt tcgaccattc
    13441 tcgatctgac ctcagggatc aggctgatcc tgctgacata tgaagagacc tccacccctc
    13501 ggatctgagt ttcgacattt tcagaattca ggaaagtctc atatcctgaa atatccggat
    13561 tgtaggcaaa ccctatattg atttccctga gatctgaaat gatatcggca gttttcagga
    13621 agtcgttccc gacatatccc ttcctgaggc aataaagggt gaccgcgcgg tacatggcac
    13681 cgctgtcaat atagatgtaa ttcatttctc tcgcgattgc cctggcaaaa gagcttttcc
    13741 cgcttgagga gtagccatct attgcaataa tgattttttt cattgcatcg gcatctgggg
    13801 tgcaaatatg gaaaatttca cgtgtataat caatttttat tttggcgccg gaattttctg
    13861 tacaactggt cggtcctggt aatcagcgaa atatgattcg atgcgcctgc aaggtgatat
    13921 gaagcatgcc caaactgcag cgacatgaag gaagtattta ttccaaaccc ccatgaaaac
    13981 ccggctcctg ccgcttttga ttctgtcatc agttcctttc ttctctggta attatagcct
    14041 gcgctcaggt agaagttctt atgcggaatc agttcagctc ccatgatcat atgcctcata
    14101 atgttttcca ttaatccgtc cggagatcct tttgctccgt taccagccgg gtctttataa
    14161 tcgtgtgtca ggtcgtattt ttccagatgc ctgattgtaa gggagaacct gaatggggca
    14221 tgggccagct ttgccgatgc acctgccagg atttcgaagg gtgttgaacg atttttctca
    14281 ccggtatagg aaataatctg gcggcccatg tttcgcagaa caattccgcc cgaataaagg
    14341 ccgtcatccg atttccacga taccccgata tcggcagcaa atcctgcaga ggtgtatctt
    14401 tcaagatgcg acagtatagg cttaagatcg attccgaccg aaaatgagga gtcaatgctg
    14461 taagagcaga taagtgaaaa tgaatattcc gatgccgtga atgatcctgt tatatttccg
    14521 gaaggatcgg cttcggtaaa agtgccgtag ttcaggtatg tgacgccccc ggcaaaatta
    14581 cctgcatttg ctgttgaacg ggaataaaga accatgccat aattaattcc tgccaggtaa
    14641 ctggcatagt taagggaaag gttcttgtcc attgccggtg aaaggagcga aggattatga
    14701 tgtgccaggt tcaggtcccc ggcaggcatc gatacgtttg atcccccgag agaggctacg
    14761 agacctgaat gggtgaggtt caggaactca tagaccctgg ttcccccggt ctgaggaaat
    14821 aattccggca ataagatgct cagggcagct atgaacagga cttttttcaa cagcagatat
    14881 atagggtaaa aataaaatat taaaacaaca gttaaaaatg ccggaaaact ctttcagctt
    14941 tcatgattaa cagggccaca ttctgttaat aaaaaattct taactgcctg tctgaattca
    15001 gttccattga ttaattttaa gttcatattc aatgtctgtt taagttctgt tggcataaaa
    15061 atttttatga atcgggtaag gcattggcag agttgttgtt cagtttgctg gagcaggttt
    15121 tttatttcgg tagctaatgg cacaggtttt gataattatt atttttcaaa tacggtgaat
    15181 aataattgta atttttttgt ctgcgatgtg gatggagacg gatattcgga tttaattagt
    15241 actgataaag attctccctg gacaacaggg taccaggttt tcagaccggg aggaaattca
    15301 acggctctga tggaaaaagt tggcaacggc ctgggagaac tgaccaaggt atcatacctg
    15361 aagctgtcaa aggcaaattc ctcttcttat cagcggggga gcagcgctgt ttttccgttg
    15421 accgattttc agggtcctct cactgttgta agctcattcc agacggacaa tggcaaagga
    15481 agcatgaatc tgatggaata ttattacgag ggagccaaaa tacatcttcg cggaaaagga
    15541 tttctgggtt ttcttaaaac gagacttaca gatcacactg ccggtatagc aaatgaagcc
    15601 attatcactg atggttttaa tccgacgtat ttttatccat ccgtgcagct gtcgctttca
    15661 aaacgagccg gtacaaccga tactatttcc aaagtaaaaa atgtactatc acaggtagtt
    15721 ctgaatgcat caaagaaatg gatcttccca tacgtctctt catcgaaggt aacaaacaag
    15781 cttacggggc atactgttac catgaacaca tcgggatatg ataattacgg aaatccttcc
    15841 actatcgtga aaagctatag cagcggtccg acagaaacat caacaattgt ttatgataac
    15901 actgtatcta caagccagtg gctcctcggc cgtccgacaa gcgctacaac tcagtacaca
    15961 gggggaggaa ataccataac cagagctgtt accaggagct tcagcactac aaccaataac
    16021 cttaacagcg agacatggta tccgggtaca ggcaatcagt ttgtgaaagg gtataaatac
    16081 aattcaaacg gaaccctgaa aagggattcg gttacagttg gcggagttta ccggacaaat
    16141 atctatacct atgaatccaa tggagtaaga ataaaaacaa ttaccgaccc gctttcccat
    16201 actgccacga ttacatatga tgcctgtggc cggctccagg ctgtcaaaga ctatctcaat
    16261 aatactcttt cataccagta tgatgaaatg ggacgggtta caaatgtatc ctcaactgac
    16321 gggaaccaga cctcatccgt ctatgcatgg gaaagccctg catctgctcc aaagcctgca
    16381 agatactcgg tagttgtaac cggaaatgac ggctcgcaga ccaaaagatg gttcgataag
    16441 ctctcaaggg aaatacgctc tgatatgaaa ggttttgacg gctcctggat ctatacagat
    16501 acaaggtata atgtaaaagg acaggttgac agtatttcgg aaccatatta tacaggacca
    16561 acctggaaca ggtaccagta tgataactat ggaaggaaga caaatctgta cagaccatcc
    16621 gggaggaaca gctcatgggt ctacagcagc aacactatta ctgaaacaac cggcggaagg
    16681 tcattctcca aaacatattc ttccgatgga accgtatctt cagccactga tgcgggagga
    16741 acaataagtt atacctatta tcctgacgga aaggtaaaaa acatcaccgc ccctggcaac
    16801 attgtaactc agatgcagta tgatattgca ggcaaccaga ccaaactcat tgatccgagc
    16861 gcaggtacaa ttaattaatt tacaacggct ttggagaatt gcaaacacag cagaattcca
    16921 gactgcaaac tacaacgata acatactatc cggatggaag aattgcccgt aaagtgacac
    16981 ccgaaggcac gacaaaatac tggtataatg caaataaaca gctgaccaat atcggttcac
    17041 caggcggcgt gagccgttcg tttgcctacg actcgaaggg aagacccgcg acgattaccg
    17101 atacgatacc cgggaccact cccctgataa aaacactgac atatgacggt tacggcaggc
    17161 caggtacaat aacacatcca tcgaatattg ttgaaaccag gaattacaat tctaacgggt
    17221 atctcagctc ggtttctgcc ggcggttcgg ccaggtggac cgtcacgtcg atgaatgcat
    17281 tgcagcaaat cacggggggc cggtacggaa cgagcctcaa cgctgcttac ggctatgaca
    17341 gctacggtta tctcacatcc aatacaacag gatctcttca gaattatact tatagtttta
    17401 ataccgttac cggtaacctg aactggagaa agaatgtact gcaggcaaat atccaggaga
    17461 atttcgagta tgataatccc gaccggctcg acagggttta ccggggagga acaaccttgc
    17521 tggacatggc ttatgaaagc aataaaggag gtataacaac aaagagcgac gcaggtacac
    17581 tgaattataa tatttcagca aaaccctatg cgttgggaac ggttaatccg tcagcagggc
    17641 tgttgcccac aacagtcgat tcactaactt atacctcctt tgaaaaagta aatacgatct
    17701 ctgaaggtaa ctttgcagca tattttacgt ataacagtga taacgagcgt gcaaagatgg
    17761 aagttaagca gaacggcagc acaatactta caaggtggta tccgagcggg ggttatataa
    17821 aagaaacagc aggaggagca accaaagaat acacatttat aggaggtgat gcctattcgg
    17881 cgcctgttgt tgcaataact cagggaggaa ccacaacgta ttacaacctg ctcagggatc
    17941 atctcggtag tataacacat gtagtaaatg catcgaacaa cacgttgctt tatgagtaca
    18001 gttatgatgc ctggggcagg atgaggaacg tgtcatcatg gaccagttat gctcccggtt
    18061 cagagccatc gttattcata gcaggaagag gatttaccgg gcatgagcat ttaccttggt
    18121 tcaacctcat aaatatgaac ggacgcgttt atgatccgct ggtcggccag tttcttagtc
    18181 cggataatta tgtacaggcg ccttatttta cacaggggct gaacaggtat gtgtactgcc
    18241 t
//
LOCUS       DAOOYG010000047        18039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_2995_length_18039_cov_7.140847, whole genome shotgun sequence.
ACCESSION   DAOOYG010000047 DAOOYG010000000
VERSION     DAOOYG010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18039
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2995_length_18039_cov_7.140847"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..632)
                     /gene="thrH"
                     /locus_tag="PK288_05855"
     CDS             complement(18..632)
                     /gene="thrH"
                     /locus_tag="PK288_05855"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF010109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HPM18478.1"
                     /translation="MYIVCTDLEGVLVPEVWINVARWTGIDELKLTTRDISDYSALMT
                     RRLEILRQHGITITDIQKVISLLDLMPGAQDFIEWLHGRLQMIVVSDTFREFADPLLQ
                     KMGWPLLFCHHLTIDGNGNITDFNLRQNDAKKMVVKKMQELNYKVIAIGDSYNDISML
                     RQAEMGILFNPPPNVINDNKDIRVVTSYMQLKNIIRDTIDNGHK"
     gene            complement(652..2019)
                     /gene="thrC"
                     /locus_tag="PK288_05860"
     CDS             complement(652..2019)
                     /gene="thrC"
                     /locus_tag="PK288_05860"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPM18479.1"
                     /translation="MENQNLYYSTNLISPEVTFREALMKGLAPDGGLYMPKHLPSVSR
                     DELESFREKKYSEIASQVLGKFLHEEADRDDISGICNEIYDFGVPIETVDGRFNIMRL
                     DRGPTASFKDFAALFMSRMMQHFLSLENRHIIILTATSGDTGSAVANAFHGMKNIRVI
                     ILFPSGEVTDIQRKQMTTLHDNIQVLAVDGKFDDCQALVKKAFMDQDLAGKHLSSANS
                     INIGRLLPQSVYYFWAWSRICGETGEKVVFSVPSGNFGNLAGGLTAWQMGLPVKKFIV
                     ATNENDEVPEYFRSGNYVPISPSRNCISSAMNVGHPSNLARIVAMFGGNMNEKGKFIT
                     EPDLGKMREIMYAVSVNDNETRETIKTCHDNSEIILEPHGAVAWRGLEKFIESGPGFR
                     IDEKLCCILETAHPAKFMDEILKVRPGGIMFPESLAKVEDVPEKFTSLENDYNIFKNF
                     IRKYY"
     gene            complement(2032..4461)
                     /gene="thrA"
                     /locus_tag="PK288_05865"
     CDS             complement(2032..4461)
                     /gene="thrA"
                     /locus_tag="PK288_05865"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HPM18480.1"
                     /translation="MKVLKFGGSSIGTPERIRSVKKIIEAESLPCVAVVSAFEGVTDS
                     LKKTSELASLHNPGYGNSLDELYSRHISFAKDLLTGQNLDIALKKIEEFFDELKEILN
                     GIFLVRELSKYSLDKVLSAGERISALIISLFFDRCILIDTREIIKTDDNFGNAAVDFE
                     SSSKLIRERIIGKNNLYCVPGFISSTSTGETTTLGRGGSDYTAAIIAAAVNAVALEIW
                     TDTDGFMTADPKKVEKAYAIRSLTYSEAIELSHFGAKVIYTPSLRPVYKKQIPVYVKN
                     SFNPKSEGTVISEKSTNGTISPIKGISSIDHIDLITLQGTAMVGVTGTSMRLFGALAN
                     KNINIVLITQASSEYSITFAIDPQYTKQASEAINLEFSREITYSRELNVLIEHDLSII
                     AIVGEKMKNTPGISAILFRSLARNGISVIATAQGSSELNISVVIRNENLKRALNAIHD
                     GFFLSRYKELHLFLAGTGNVGSSLIKQLRKQHDILLKDHNLKLNLVGLINSRHMLLDS
                     KFIPLDKCTEMLSSKGEKSDIGLFISRMAKLNLRNSVFIDCTADARVGAIYGDVLEKY
                     VSIVAANKIACSSDYSYYGKLKALANERGVRFMYETTVGAGLPIIKTINDLVVSGDRI
                     LKIEAVLSGTLNFIFNTISPEIPLSSAIRMAKEKGISEPDPRIDLSGKDVVRKIMILS
                     REAGYPVEEKDVIVKKFLPESCFKGSLASFYEKVRELDPAFERERRELEQSHRKWRFY
                     AIMDQGKASVELITVDSSHPSYNLEGSNNIVLLTTERYRELPMVIKGYGAGADVTAAG
                     VFADIMRVVNV"
     gene            complement(4622..6457)
                     /locus_tag="PK288_05870"
     CDS             complement(4622..6457)
                     /locus_tag="PK288_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM18481.1"
                     /translation="MLKNHPKGLLVAFFSNMGERFGFYTMMAILVLFLQARYDLPAKD
                     AGDIYSWFYFAIYALALVGGILADSTKKYKLVILTGIIIMFAGYVVMAIPGMSLTVTI
                     IGLMTIALGNGLFKGNLQAVVGQMYDNPAYSKLRDTAYMIFYMGINVGAFFAPFAANG
                     MRNWWLKVNGFAHDASLPALCHRYIKGTLPDTAVLQELADKVSLRGPVSDLAAFASGY
                     LDVFSKGYNYAFGIAAVAMVISLLVYIIFNKLLPSKEVRAAKVSSERIEFRPAPFIAA
                     LGAMVVTAFGLHFIRQVGWAAGFAFGVFAAFVTWIILTSHKEERDRITSLILVFFVVI
                     FFWMSFHQNGLTLTLFARDYTLKQVGPFTNLFFTLPSMLAVIGSITGIVILLYKNIKT
                     KHKIAGGVLFLISFWLAAFYVTGFDFTTILKKNLLPFGSQNSIAPEVFQSFNPLFIVA
                     LTFPVMGAFAWMNRKGIEPSTPKKIGIGMIIASIGFIIILIGSLHAPSPHSLAGAPVS
                     DADRVSPYWLISSYLVLTIAELFLSPMGLSFVSKVAPSRFQGLMQGGWLLATAVGNKL
                     LFIGTLLWDRVALGTLWLVFIVCCLLSATFIFSILKRLEKASSEN"
     gene            6648..8165
                     /locus_tag="PK288_05875"
     CDS             6648..8165
                     /locus_tag="PK288_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPM18482.1"
                     /translation="MKIFTCAQIKEIDEITIRTEPISSFNLMERAAGQLLLWYLKTFD
                     RARRVLVFVGPGNNGGDGLALARMLALNRYNVGVYYLVISERTSPEWKLNHQKLRQET
                     NVPFISISRRDQIPDFRPDDVLVDAVFGSGLTRPAGGLASDIIRLINQTGCTVVSIDI
                     PSGLFGEDNGQNDPESIIMADYTLTFQFPRLSFMFAENAKYTGEWIVLPIGLSNSAIN
                     NTETPYSFVQADALSTSLKIRKRFDHKGIYGHGMLIAGSGGKMGAAVLGARAALRTGI
                     GLLTCHVPGAGNQIIQTCVPEAMSQPDSNNEIISEIPGITLFDAIGIGPGIGTDPVTQ
                     KAFQSVLLNRSVPIVIDADGLNILGLNDKWLSVLPEKTVLTPHLKEFERMAGKSDNGY
                     MRLKNQISFSAKYNCIVVLKSAFTSVSSPDGRVSFNSTGNPGMATAGSGDVLTGMILA
                     LLAQGYSPENAAVIGVYLHGLAGDIAAEKDSPDSIIASDIIENIGNAFKQLKGSF"
     gene            8240..9364
                     /locus_tag="PK288_05880"
     CDS             8240..9364
                     /locus_tag="PK288_05880"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HPM18483.1"
                     /translation="MKVKSRITILVLLAAFAGACSPSGKLKESGAVISPVSDRMAFSD
                     GSLIYGLPLSVLDIKIDAVRIIEKPGPYARFAEDYLGLRDVIKTEREYWKIRTITVSS
                     HSELDPSQFYMIETSNTFRTNALTLKKEGLILDLNPDLYNNGARYFAGAPADYSSSRL
                     FDLGADEYFQERRDTAYKVVNVDTAFIRIPYLVEKKQKLSYDQLAERAARRLMEMRDG
                     KHLILSGETNVFPQNEYAINEMNRMEKEYTDLFAGKIWRESHSFQYQIIPEKGTTGRQ
                     ITICRFSETSGPTASAGEGTPLMIEFLPESKTRRLEIINRNTSSSSSRKSRLDKLYYR
                     VPEIVQIKITYGGELLNNTRHLIYQFGEVVQLPSNFIIGK"
     gene            complement(9371..9901)
                     /locus_tag="PK288_05885"
     CDS             complement(9371..9901)
                     /locus_tag="PK288_05885"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HPM18484.1"
                     /translation="MIKKKIGLMFLAALTLVTYSQNAQVRWYTLQEAERLSKQSPRPI
                     FIDTYTDWCGWCKKMDQETFTNNVIAEILNTRFYPVKFNAEGKESVTFLGQTFVNDGK
                     SGRTHQLAIALLQGQLSYPTVVFLTKQDDQFSVSPVPGFRQPKDMEMLLSFFADQAYL
                     SQKWEDYQRSFKGRIQ"
     gene            10090..11472
                     /locus_tag="PK288_05890"
     CDS             10090..11472
                     /locus_tag="PK288_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM18485.1"
                     /translation="METIRRTLRDSKSARWIALAIVSFTMLCGYYLTDVMAPLKPLLE
                     KELLWNSAEYGFFTSAYGWFNVFLLMLIFGGIILDRMGVRFTGKMATIIMVIGTAVKY
                     WAVSTHSLDGSVILGMKAQVVIAGLGFAIFGVGVEVAGITVSKIIVKWFKGKEMALAM
                     GLEMATARIGTTLAMVSSVPIARALNGVSKPILVCLIMLCIGMIAFFLYAIMDKRLDQ
                     SLQLAAAENSEPLSEESFNVRDIVYIITNKGWWFIAILCVLFYSAVFPFLKYATDLMV
                     NKFNVRPDLAGIIPGMLPFGTMILTPLFGNMYDRKGKGASIMLLGALLLIFVHAMFSI
                     PFLSATPLAIALIIILGIGFSLVPSAMWPSVPKIIPEKQLGTAYAMIFWVQNIGLMGV
                     PALIGWVLNKYCITGTHLIDGVEVPSYNYTLPMIIFTCFGIIAFVFALLLKAEDKRKG
                     YGLELPNIKK"
     gene            11552..11884
                     /locus_tag="PK288_05895"
     CDS             11552..11884
                     /locus_tag="PK288_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPM18486.1"
                     /translation="MNPNEFEKAKIFSFPESIEYSDGGIVSKTVLKKSTGNISLFSFA
                     AGEGLSEHTAPFDAMIQVVEGKGEIIIGGKSFILNAGQVIIMPANIPHSVKAAEKFKM
                     VLTMIRSN"
     gene            complement(11956..12606)
                     /locus_tag="PK288_05900"
     CDS             complement(11956..12606)
                     /locus_tag="PK288_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPM18487.1"
                     /translation="MNIDKLLKFFVPKDHSFFPLFVEDAKNLVKAARLLNELLSSTEL
                     DDHERINKQIKELEHIGDQITDKIFQQLNKTFITPFDREDIHELAGSIDDVVDSINGI
                     SRTVCLYKPKSIMPVCGQLAQMILEGTGEINNCVINLNDAAANKDEITRACNRVTDIE
                     HQADEIYFEAVSELFEKESNPVEILKTNKILERLERCVDEEEDVTDVIKSILIKLA"
     gene            complement(12639..13637)
                     /locus_tag="PK288_05905"
     CDS             complement(12639..13637)
                     /locus_tag="PK288_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPM18488.1"
                     /translation="MTFLHFIITLVLIFDFINGFHDSANSIACIVSTKVLSPLAAVSI
                     AAFFNFFAYLVFHLKVASTIGKGVVETDAININVIGAALIAAITWNLFTWWWGLPSSS
                     SHTLVGGLIGSAVASSGFGSIITAGVMKIVAFIFIAPALGFIFSFFLSSLIMWILRNV
                     PAFAVDKWFRRLQIISASAFSLGHGGNDAQKSVGIIWVAMIISGLVTKEATEPPGWVV
                     VSCYSAIALGTLFGGWRIVKTMGQKITKIKPFEGFCAEAAGTLTLVGATHWGIPVSTT
                     HTITGAIMGVGARKRLSAVKWGVTRNIFWAWILTIPVSAIIGGIMYYFLVWFNPLY"
     gene            13945..15402
                     /locus_tag="PK288_05910"
     CDS             13945..15402
                     /locus_tag="PK288_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPM18489.1"
                     /translation="MNDIRNLEPKSLWSYFYDITRIPRPSKKEKQITEFILKFAGQYK
                     LESLVDKTGNVILRKNATKGMENRKGVILQAHLDMVPQKNSDKVHDFEKDPIETIIDG
                     EWVRANGTTLGSDNGIGVAAAMAVLASDNIVHGPLEVLLTVDEETGMTGAFGLKKGIL
                     RGDILLNLDSEDDGEFNVGCAGGIDVSATGRYGQVKCPGGMTAYQIMIRGLKGGHSGV
                     DIALGRANSNKLLFRFLMQAESDFRIRLAEAAGGDLRNAIPRESQAIVLVPSASEGKF
                     EKFVKGYNKMYKDEFAGVEPTISITCKKTERPAQVMSIKDQYGIIRSVFACPNGVQRM
                     SPAVEGLVETSNNLAIVKCSKGKYEVHNLTRSSVDSAKEATAWKIAAIFHLIGAKISL
                     YGSYPGWKPNMDSPILKTMTNVYRKMFGEEPEIKAIHAGLECGLIGGIYPRLDMISFG
                     PTIKYPHSPDEKVLVPTVKKFFDFLCETLKKIPEK"
     gene            complement(15509..16039)
                     /locus_tag="PK288_05915"
     CDS             complement(15509..16039)
                     /locus_tag="PK288_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPM18490.1"
                     /translation="MSDKKPGLNKLYALSSENKVFCFPRPLKHSIKIGKRVYDVRLKT
                     DDKFGTYILWKNRKYPVEIVRSRQNKYEILFNDISYTFTVETPFSLKRLKVLGSGRGK
                     AEKELVKAPMPGKIIDVLVREGASVLRGEPVIILEAMKMQNEIQSPVNGRVTAVNTRP
                     NTNVMKDDPLLEIKIE"
     gene            complement(16053..17558)
                     /locus_tag="PK288_05920"
     CDS             complement(16053..17558)
                     /locus_tag="PK288_05920"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPM18491.1"
                     /translation="MKIKRILIANRGEIAVRIIKTAARMKISTIVIKTAVEPNAMYVS
                     MADIVYDNTEDTSDIPVFLNIDKLIEIAGKTNADAVHPGYGFLAENAYFAQKCIDNNI
                     IFIGPSPDAIYKMGNKTIARRIALASGIPMAVGSEGNIGNEDEAMAVAGKIGYPIIIK
                     AASGGGGRGMRIVRNPQEMDRMFIIASKEAETAFNDPSVFIEKYIENPKHIEFQILGD
                     TAGNIIHLGERDCSIQRKHQKLLEEAPSVALDEELRKTMGNMAVRIAAAVNYYSAGTV
                     EFLLDSDKNFYFMEMNTRIQVEHPVTEIVTGIDLIEQQILVANGDKLKIRQNDVNLKG
                     WAIECRINAEDVQSGFAPVPGRIENLSLPSAPYIRVDTGVQAGSSVVANYDSMIAKLI
                     ISGRDRKDAIRNCKMALDKVWIKGTKTTLPFFRMLVRNQKFLNGDFTTAFIEKDLDKY
                     FLSSEYEEMLAAWLATKLFIEENFLEDSIPVDFEHTSEMSPWLLNKRINQF"
     gene            complement(17668..>18039)
                     /locus_tag="PK288_05925"
     CDS             complement(17668..>18039)
                     /locus_tag="PK288_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPM18492.1"
                     /translation="PKITIILRKAYGGAFIVMNSKNLGGDFNYAWPSAEIAVMGPDGA
                     VAILYRKELAQSKNPAELKKELLKKYRDKVANPFIADELGYIDEVIDPAVTRKKVISA
                     LSALSNKWVKVPARKHGNMPL"
BASE COUNT         4973 a         4044 c         3867 g         5155 t
ORIGIN      
        1 atcttctttt ctgaccccta cttatggccg ttatcgattg tgtcccgtat tatgttcttg
       61 agctgcatat atgaggtgac caccctaata tccttgttgt cgttgataac attaggtggc
      121 ggattgaaca agattcccat ttcagcctgc cggagcattg aaatatcatt ataggaatca
      181 ccgatggcaa taaccttata attgagttcc tgcatttttt tcaccaccat ctttttggca
      241 tcattctgac ggagattgaa gtcggtgata ttaccattac cgtcgattgt gagatggtga
      301 cagaataaga gaggccagcc cattttctga agcagcggat cagcaaattc cctgaaggtg
      361 tctgacacaa ctatcatctg gagccttccg tgcagccact cgataaaatc ctgtgcacca
      421 ggcatcaggt caagaagaga tatcactttc tgaatgtcgg tgatggtgat tccgtgttgt
      481 ctgagaatct caagcctcct tgtcatcaga gcgctgtaat cacttatgtc tcgcgtggtc
      541 agcttcaatt catctatacc ggtccatctc gccacattta tccagacttc cggtaccaaa
      601 actccctcca gatcagtgca aacaatgtac atttttccag tttctgttta attagtaata
      661 tttcctgata aaatttttaa agatattgta atcattttcc agtgaagtaa atttctccgg
      721 aacatcctca acttttgcca gcgattcggg aaacatgatt ccgccaggtc tgactttaag
      781 tatttcatcc ataaattttg ccggatgtgc cgtttcaagg atgcagcaaa gcttttcatc
      841 gatccggaaa ccgggtcctg actcaatgaa tttctccagg cctctccatg ccacagcccc
      901 atgcggttca agaatgattt ccgaattgtc gtggcatgtt tttattgttt cacgtgtttc
      961 attgtcattc acactgaccg catacatgat ctcgcgcatt ttccccagat caggttctgt
     1021 tatgaatttc cctttctcat tcatgtttcc gccgaacatt gccacaattc ttgccaggtt
     1081 actcggatga cccacattca tggcactgga aatacagttc cgggaaggtg agattggaac
     1141 atagttcccg gacctgaaat attccggcac ttcgtcattc tcatttgtag caactatgaa
     1201 ttttttcaca gggagaccca tctgccacgc ggtaaggcct cctgccagat tgccgaagtt
     1261 cccgcttgga acgctgaaaa caaccttttc tcctgtttca ccgcaaatcc tcgaccatgc
     1321 ccagaaataa tatacggact gaggaagaag tcttcctata ttgattgaat ttgcagatga
     1381 cagatgtttt ccggcaagat cctgatccat aaaggctttc tttaccagag cctggcagtc
     1441 atcaaattta ccgtcgacag caagcacctg tatattgtca tgcagagttg tcatctgttt
     1501 tcgctggatg tcagtgacct cacctgaagg aaataaaatg attacacgga tgttcttcat
     1561 cccgtggaag gcattggcta ccgcactccc cgtatctcct gaggttgctg tcaggataat
     1621 aatgtgcctg ttttccaggc tcaggaaatg ctgcatcatt ctgctcatga acagggcggc
     1681 aaaatctttg aatgaagcag tcggccccct gtcaagacgc atgatattaa acctgccgtc
     1741 aactgtttcg atggggaccc cgaaatcata aatttcatta catattccgg atatgtcatc
     1801 cctgtctgct tcctcgtgaa gaaactttcc caaaacctgg gaagctattt cagaatactt
     1861 tttctccctg aaagattcca gctcatcgcg ggaaactgaa ggcagatgct tcggcatgta
     1921 taatccgccg tcaggcgcca ggcctttcat cagggcttcc ctgaatgtaa cttccggcga
     1981 aatgaggttt gttgagtaat atagattctg attttccacg acattaacgg tttaaacatt
     2041 cacaaccctc attatgtcag caaaaactcc tgctgcagtc acatctgcac cagcgccata
     2101 tcctttgatg accatcggga gctcgcggta acgttcggtg gtcagcagta caatattatt
     2161 gctgccttcg agattgtagg atggatgcga tgagtcaact gtgatcagct caacacttgc
     2221 cttgccctga tccatgatcg catagaacct ccatttccta tggctttgct ccaactccct
     2281 gcgttcccgt tcaaatgccg gatcaagttc cctgaccttc tcatagaaac tggcaaggct
     2341 ccccttgaaa caactttccg gaaggaactt cttaacgatc acatcctttt cttcaacggg
     2401 atacccggct tcccgcgata atatcataat ttttctgaca acgtccttgc cactcagatc
     2461 aatccttgga tccggctcgg aaattccttt ttccttcgcc atccggattg cagaactgag
     2521 aggtatttca ggacttatgg tgttaaatat gaaattaaga gtgcctgaga ggacagcctc
     2581 aattttgaga attctgtcac cacttacaac aagatcattg atggttttga taattggcag
     2641 acccgcacct actgtggttt catacataaa ccttacaccc ctttcattcg caagggcctt
     2701 aagcttacca taatatgaat agtctgatga gcaggcaatt ttatttgctg ccacgattga
     2761 aacatatttt tcgaggacat ctccgtatat ggcaccgacc cttgcatctg cagtgcaatc
     2821 aatgaaaacc gaattccgca gatttaattt tgccatccgg cttataaaaa ggccaatatc
     2881 actcttttcg ccttttgaac tcagcatttc agtgcatttg tcaagaggga tgaatttgct
     2941 gtccagaagc atatgcctgg aattaatcag acccacaaga tttagtttga gattatggtc
     3001 cttcaggaga atgtcatgct gtttcctgag ttgtttgata agacttgaac caacatttcc
     3061 ggttcctgca aggaaaagat gcaactcctt gtatcttgaa agaaagaaac cgtcatgaat
     3121 agcattcagg gcacgtttga ggttttcgtt ccttatcaca acagaaatgt tcagttcgga
     3181 tgagccctgg gctgttgcaa tgacacttat cccgtttctt gcaagtgatc tgaaaaggat
     3241 tgcagatata cctggtgtgt tcttcatttt ctcaccaact attgcaatta ttgacagatc
     3301 gtgttctatc aatacattga gctccctgct gtatgttatt tcacggctga attccaggtt
     3361 gattgcctcc gaagcctgct tcgtatattg gggatcgatg gcaaaggtga tcgaatattc
     3421 ggaggatgcc tgggtaatga gaactatatt tatattttta tttgccaggg cgccgaaaag
     3481 cctcatcgat gttccagtaa cacctaccat ggccgttccc tggagagtga tcagatcaat
     3541 gtggtcaatg gaagaaattc cttttatggg gctgattgtc ccgtttgtgg atttttcgct
     3601 tatgacggtc ccttccgact tcgggttgaa ggaattctta acataaaccg gtatttgttt
     3661 tttatatacc ggacgaaggg aaggagtgta aatgaccttt gccccgaaat gggacaattc
     3721 aatggcttct gaatatgtga gcgaacgtat tgcatacgct ttttcaacct ttttgggatc
     3781 ggctgtcatg aatccgtcgg tgtcggtcca tatttcaagt gcgactgcat taacggctgc
     3841 agcaattatg gctgcggtgt agtctgatcc tcctctgccc agcgtggttg tttcacctgt
     3901 tgaagtactg gaaatgaatc ccgggacaca atataaattg ttttttccta tgattctttc
     3961 ccgtatcagt ttgcttgacg attcaaaatc cacggctgca ttgccgaaat tatcatcagt
     4021 cttgattatt tcccgtgtat ctataagaat acacctgtcg aaaaacaatg aaattatcag
     4081 ggcagagatc ctttcaccgg cactgaggac tttatcaagg gaatatttac tcagctccct
     4141 taccaggaat atcccgttca gaatttcttt cagctcatca aagaattctt caattttttt
     4201 cagtgcaata tcaaggttct gcccggtaag cagatctttg gcaaatgaaa tatggcggct
     4261 gtacaattca tcaagactgt taccgtaccc cggattatga agtgaggcaa gctcacttgt
     4321 ttttttcagt gagtcggtaa ctccctcaaa ggcagatacg acagcgacac atggtaaaga
     4381 ttccgcttca ataatctttt ttacactccg tattctttca ggagtaccta tagaagaccc
     4441 gccaaatttt aaaaccttca taaatcttaa agtgatttct ttaacatccc gggggatgtc
     4501 agaaattaaa aagcacaaaa atagcaaaaa tcaggataat tatttaatta attgaaatta
     4561 ttaactaaac atgcctttct tgctgaaaac agatggacag gatcccttat taactaacag
     4621 atcagttttc agacgaggct ttttccagtc tttttaaaat tgaaaaaatg aatgtggctg
     4681 acagcaaaca gcatactatg aacacgagcc agagggttcc aagggcaacc ctgtcccaca
     4741 gtaatgttcc tataaacaga agcttgttgc ctactgctgt tgccagcagc cagccgccct
     4801 gcatgagacc ctggaaccgc gatggcgcca cctttgacac aaatgacaat cccatgggac
     4861 taaggaataa ctcagcaata gtcagtacaa ggtaactgct tatgagccag tacggtgaaa
     4921 ccctgtcggc gtcagaaacg ggggctcctg ccaatgaatg tggcgatggc gcatgcaaag
     4981 agcctatcag tatgataata aatccaatgg aagcaattat catgcctatg ccaatctttt
     5041 ttggtgttga cggttctatc cctttcctgt tcatccatgc aaatgcaccc ataaccggga
     5101 atgtcagcgc tacgataaac aatggattga atgactgaaa tacctccggt gcaatggaat
     5161 tctgtgagcc gaatggcagc aggttcttct tcaggattgt tgtaaaatca aatcccgtta
     5221 cataaaatgc cgcgagccag aaagagataa gaaataaaac tcctcctgct attttgtgtt
     5281 ttgttttaat gtttttatat agcaggatca caattcctgt aattgacccg atcacagcga
     5341 gcattgacgg aagggtgaag aaaagatttg tgaagggacc gacctgcttg agggtatagt
     5401 cccgtgcaaa taatgtaagt gttaatccgt tctggtgaaa ggacatccag aagaatatga
     5461 caacaaaaaa taccaggatg agcgatgtta tccgatccct ttcttcctta tgcgaagtca
     5521 ggataatcca ggtaacgaat gcagcaaaaa ccccgaaagc aaatcctgcc gcccatccga
     5581 cctgcctgat aaagtgaagt ccgaatgcgg tgaccaccat ggcgccaagt gccgcgatga
     5641 acggtgccgg cctgaattca atcctttctg aagatacctt tgccgcccta acttccttcg
     5701 aaggaagcag cttgttgaaa ataatatata ccaggagaga aattaccatc gcaacagcag
     5761 ctatgccaaa tgcatagtta taaccctttg agaaaacatc aagataaccg gaggcaaatg
     5821 ctgccagatc agataccggc cccctgaggc tgaccttgtc tgcaagctcc tgcaggactg
     5881 cagtatccgg aagggtccct ttaatatacc gatgacacag tgccggaaga ctggcatcat
     5941 gtgcaaaccc gttgacctta agccaccaat tgcgcattcc gttggcagca aagggtgcaa
     6001 aaaaggcacc gacatttatt cccatgtaaa atatcatata tgccgtgtcc cgaagcttgg
     6061 agtaagcagg attgtcgtac atctgcccta caaccgcctg caggttgcct ttgaacaatc
     6121 cattacccag ggcaatggtc atcagcccaa tgatcgtgac ggtcagtgac attccgggta
     6181 tcgccatcac cacgtaaccg gcaaacatta ttataatgcc tgtgagaatt accagcttgt
     6241 acttctttgt tgaatcagcc aaaattcctc ccacaagggc gagtgcatat atggcaaagt
     6301 aaaaccagct gtaaatatca cctgcatctt tggccgggag gtcatacctt gcctgtaaaa
     6361 acaatacaag tattgccatc atggtataaa aaccaaatct ttcccccatg tttgagaaaa
     6421 aagcaaccag taaacctttt ggatgattct tcagcatata attctgtttt agagtttaag
     6481 gatctgcggg aaataaccca aaatatacaa tcagaaaatc aggtttatca ctaaatgcaa
     6541 ctatttcaag gcggaagcaa atatattata tcttttttac agtaaaaaat caacttagtt
     6601 ttaaataccc gagtaagatc gtaattttgc aggtaattga gcggccaatg aagattttta
     6661 cctgtgcaca gataaaagag atagatgaga taactatcag gactgaaccc atttcctctt
     6721 ttaaccttat ggaaagggca gccggtcagt tgcttctttg gtatttgaaa accttcgacc
     6781 gggcccgcag agtgcttgtc ttcgtgggcc ccggaaacaa tgggggcgat ggtctggctc
     6841 ttgccagaat gctggcactc aacagatata acgtcggcgt ttactacctt gttatttcgg
     6901 agagaacttc gcccgaatgg aaattaaatc atcagaagct caggcaggaa accaatgtgc
     6961 cttttatctc tatttcgcgc agggatcaga ttcccgattt tcgtcccgac gatgtcctgg
     7021 tagatgcagt gttcggatcc gggctgacca gaccggccgg ggggctggca tcagatatta
     7081 tcagattaat taatcagacc ggctgtacag tagtatccat agatatcccc tccggacttt
     7141 ttggtgagga taacggacaa aatgatcctg aatcaataat aatggccgac tataccctga
     7201 cttttcagtt tcccaggctt tcgtttatgt ttgccgaaaa tgcaaagtat acgggtgaat
     7261 ggatagtgct tcccataggt ttgagcaaca gtgcaataaa taatactgaa acaccgtact
     7321 cctttgtaca ggcagatgcc ctgtcgactt ctttaaaaat cagaaaaaga ttcgatcaca
     7381 agggaattta cgggcacggc atgcttatag ccgggtcagg gggaaaaatg ggcgctgcag
     7441 ttcttggcgc cagagctgcc ctgagaacgg gtatcggcct gcttacctgt catgttccag
     7501 gcgctggaaa tcagattatt cagacttgcg ttccggaagc catgtcacaa ccggattcaa
     7561 acaatgagat aatttcggaa attccgggta ttacactgtt tgatgcaatt gggattggtc
     7621 cgggtattgg cactgatcct gtcacacaga aagcctttca gtcagtgctc ctcaacaggt
     7681 cggtcccgat agtgatcgat gccgatggac ttaatatcct gggattaaat gacaaatggt
     7741 tgtcagtttt acccgaaaaa accgttctta ccccgcatct gaaagaattt gaaaggatgg
     7801 ccggtaaatc agataacggg tatatgcggc ttaaaaatca aattagtttt tcagcgaaat
     7861 ataattgtat tgttgtcctt aaaagtgctt ttacctcagt ttcaagccct gatggcagag
     7921 tgtccttcaa cagtaccggt aatcccggta tggcaactgc cggcagtggt gatgtactta
     7981 cgggaatgat ccttgcactt ctggctcagg gatactcccc ggaaaatgcg gctgtcatcg
     8041 gagtatacct gcatggactt gcaggagata tcgctgctga aaaagacagc cctgattcaa
     8101 taattgcctc cgatataatt gaaaatatcg gaaatgcatt caaacagctg aagggatcct
     8161 tttgacgcca tctgtttggc attggaaaca aaaccgtgat tattttttga aaccttttat
     8221 aattaaagaa aaaaattcca tgaaagtaaa atccagaatc accatccttg ttttattagc
     8281 tgcttttgcc ggagcctgtt caccttcggg aaaactcaag gagtcaggcg ccgttataag
     8341 tcccgtttca gatcgtatgg ccttttcaga cggatcattg atatacgggc tgcccctttc
     8401 tgttctggac ataaaaattg atgctgttcg gatcattgaa aagcccggac catatgcacg
     8461 ctttgcagaa gactatcttg gactgaggga tgtgataaaa actgagcgtg aatactggaa
     8521 aatcaggact ataaccgtta gttcccattc ggaactggac ccgtcgcaat tctacatgat
     8581 tgaaacaagt aatactttcc ggacaaatgc gctcacttta aaaaaggaag ggctgattct
     8641 tgatcttaat cccgatttgt acaacaacgg ggccaggtat tttgccggag ctcctgctga
     8701 ttacagctct tcaaggctct ttgacctggg agctgatgaa tattttcagg aaagacggga
     8761 taccgcttac aaagtcgtga atgtggatac cgcttttatc cggattcctt atcttgttga
     8821 aaagaagcag aaactatcat atgatcagct tgcggaacgg gctgccaggc gactcatgga
     8881 gatgagggac gggaagcatc ttatactttc cggggaaacc aatgtttttc cccagaatga
     8941 atatgccatc aatgagatga accggatgga aaaggaatat accgatctct ttgcaggtaa
     9001 aatatggagg gaatcccatt cttttcaata ccagatcatt cccgaaaagg gaacaacagg
     9061 ccgacagata acaatctgcc gcttctccga aaccagcgga ccaactgctt ccgccggcga
     9121 aggtacccca ctgatgattg aattcctgcc cgaatcgaag accagacgcc tggaaataat
     9181 taaccggaat acttcatcat cttcttccag gaaatccaga ttagacaaac tctattacag
     9241 ggttccggag attgtgcaga taaaaatcac atacggagga gaacttctga acaacacccg
     9301 ccatctgatt tatcagtttg gggaagttgt gcagctgcct tcaaatttta ttatcgggaa
     9361 ataattgtaa ttactggatc cttcctttaa agctgcgctg ataatcttcc catttttggg
     9421 aaaggtacgc ctgatcggca aaaaatgaca aaagcatttc catatctttg ggctgcctga
     9481 atcccggaac gggggatact gaaaactgat catcctgttt cgtaaggaaa accactgtcg
     9541 gatacgacag ctggccctgc aggagtgcaa ttgcaagctg atgggttctt ccgctctttc
     9601 cgtcgttgac aaaggtctga ccgagaaaag tgacgctttc ttttccctct gcattgaatt
     9661 tcaccggata gaacctggta ttcaatattt cagcaataac gttgtttgtg aaggtttcct
     9721 gatccatttt cttgcaccat ccgcaccagt cggtataggt gtcaataaag atagggcggg
     9781 gtgactgttt gctgagtctt tctgcttcct gaagggtgta ccatcttacc tgggcattct
     9841 gggaatatgt aactaatgtt aatgctgcca gaaacattaa gcctattttc ttctttatca
     9901 tatgtatttg atcttataca gaattctttc caaaataaat caatttttac caatgttttt
     9961 aaagcacacc gtacttttat gctttaaaac aatgtatatc tgaaaatgtt cgataaactt
    10021 gcgactggcg gaagtagcat tggaatattt ttttacattt gacgtttctg aatttcatcg
    10081 cctgaacata tggaaacaat aagaagaaca ttgcgcgatt ccaaaagcgc cagatggata
    10141 gcccttgcta ttgtctcatt taccatgctc tgcggttatt atcttacaga tgtcatggct
    10201 ccgctgaagc cgcttcttga aaaggagctg ctttggaaca gcgctgaata tggctttttt
    10261 acaagcgcct acgggtggtt taatgtattt ctcctgatgc tgatcttcgg cggaatcatt
    10321 ctcgacagaa tgggggttcg gtttaccgga aaaatggcaa caataattat ggtgatcggc
    10381 actgctgtta aatactgggc agtatcgacg cattcccttg acggttctgt aatactcgga
    10441 atgaaagccc aggtggtgat tgcgggcctc gggtttgcca ttttcggggt aggagtagag
    10501 gttgccggga taacagtttc caagattata gtaaagtggt tcaagggaaa agagatggca
    10561 ctggccatgg gacttgaaat ggctactgcc agaataggaa ccaccctggc catggtatca
    10621 tcagtcccta ttgcacgtgc cctgaacggc gtttcaaaac caattcttgt ctgtctcatt
    10681 atgctgtgca tcggaatgat tgcctttttc ctttacgcaa ttatggataa gagacttgat
    10741 cagtcgctcc agctggctgc agcggaaaac agtgaacctt tatctgaaga atcattcaat
    10801 gtcagggata ttgtttacat cattaccaac aagggctggt ggttcattgc catcctgtgt
    10861 gtactgttct attcggctgt ctttccattt ttaaaatatg ctaccgacct tatggtcaat
    10921 aaattcaacg ttaggcccga tcttgccggg ataattccgg gtatgctgcc tttcggaacc
    10981 atgattctta cccctctttt cggaaatatg tacgacagga aaggaaaggg tgcctcaatt
    11041 atgctgctgg gggcactgct tctgatcttt gtgcatgcaa tgttttcaat tcccttcctc
    11101 tcggcaactc cccttgcaat cgccctgata attattcttg gaataggatt ctcgcttgta
    11161 ccgtctgcga tgtggccctc agttccgaaa ataattcctg aaaaacaact gggtacagct
    11221 tatgccatga ttttctgggt ccagaacatt ggattaatgg gcgttcctgc ccttattggc
    11281 tgggtgctca acaagtactg tatcacaggt acacatctga tcgatggcgt tgaagttccc
    11341 agctataatt atacgctgcc aatgatcatt tttacctgct ttggcataat agcttttgtt
    11401 tttgcactgc tgctgaaggc tgaagacaaa agaaaaggtt atggccttga attacccaat
    11461 ataaaaaaat aaggatgccg atattctgat tgcaggtttg cacaaaattc aattgacgtg
    11521 aatcattcaa taattaaaca aatacgatct tatgaatccg aatgaatttg aaaaggccaa
    11581 aatcttcagt ttccctgaat cgatcgagta ttctgacggg ggaatagtta gtaaaaccgt
    11641 tttgaagaaa tcaaccggca acatttctct tttttctttc gctgccggtg agggattaag
    11701 cgaacatacc gcaccgtttg atgcaatgat acaggtcgtg gaggggaaag gtgaaatcat
    11761 aataggaggt aaatcattta ttctcaatgc cggccaggtg attataatgc cagcaaatat
    11821 acctcattcg gtgaaggctg cggaaaagtt caagatggtc cttacaatga tcaggagcaa
    11881 ttgatctgac cggagtatcg cgggactaag ggcgggaaaa ggagtttcgg ttttttcatg
    11941 ccgatattct gataatcagg ccagttttat cagaatggat ttaatgacat cggtaacatc
    12001 ctcctcctca tcaacgcatc tttccagtct ttcgagtatc ttgttggttt tcaggatctc
    12061 cacggggttg gattctttct caaataattc agaaactgcc tcgaaataga tctcgtccgc
    12121 ctgatgttca atatcagtca ccctgttgca cgcccgggtt atctcgtcct tgttggcagc
    12181 agcatcatta aggttaatga cacaattgtt tatttcgcca gtaccttcga ggatcatctg
    12241 ggccagttgc ccgcataccg gcattatgga tttgggtttg tacaggcaga cagttctgct
    12301 gatgccattt atggaatcaa caacgtcatc aatgcttccg gcgagttcat gaatgtcctc
    12361 tcggtcgaaa ggagtgatga atgttttgtt tagttgttga aatatcttat cggtgatctg
    12421 atccccgata tgctcaagct ctttgatctg tttgttgatc ctttcatgat catccagctc
    12481 tgtgcttgac agcagctcgt taagcaatct ggcagctttt accaggttct ttgcatcctc
    12541 cacaaacagc ggaaagaaag aatgatcttt gggaacgaaa aatttaagca gtttatcgat
    12601 attcatgtga tccgttttta atgtttaagt ctcttcaatc aatacaaagg attgaaccag
    12661 acaaggaaat aatacattat tcctcctata attgcagaaa ccgggatggt aaggatccag
    12721 gcccagaaaa tgttccgggt cacaccccat tttacggccg ataagcgctt ccgggcacct
    12781 acccccataa tcgcacctgt gatggtatgt gttgtactta caggaatacc ccagtgcgtg
    12841 gctccaacaa gtgtgagcgt tccggcagcc tcagcgcaaa aaccttcaaa aggcttgatt
    12901 tttgtgattt tctgccccat ggtcttaaca atgcgccatc ctccaaacaa agtgccgagt
    12961 gctatggcgg aataacatga cactacaacc catccggggg gttccgtagc ctctttggtt
    13021 acaagtcctg agattatcat tgccacccat atgataccca ccgacttctg cgcatcattg
    13081 cctccatggc ccagactgaa tgcagacgcc gatatgatct gaagccggcg aaaccatttg
    13141 tcaaccgcaa atgcaggaac attccgaaga atccacatta taagtgaaga gagaaaaaag
    13201 gaaaagataa atccgagagc aggagcaata aagataaatg ctacgatctt cataactcct
    13261 gcggtgatta ttgatccgaa tccggaactt gcaacagctg agccaatgag cccgccaacg
    13321 agggtgtgtg acgaactcga tggcaggccc caccaccagg tgaacaaatt ccaggttatg
    13381 gcagcaataa gggcggctcc gatgacatta atattaattg catcagtttc aacgacacct
    13441 ttaccgattg ttgaggcaac tttcaaatga aaaaccaggt atgcaaaaaa gttaaaaaat
    13501 gctgctatgg atacagcagc aagtggtgaa agtacttttg tggaaacaat acaggcaatg
    13561 gagttggcag aatcgtgaaa gccgttgatg aaatcgaaaa tgagaacaag ggtaataata
    13621 aaatgcagga aagtcatctg ggtgttacag tataaataat tgatcctttg gatattgttg
    13681 attgaaaaga atgcatttta ttcagccact tccgttttat tccttctgtt gcgtcaatca
    13741 ggcagacaaa aataaaaaaa ttgtcaacaa gtgcaaaata aatagttttc aagttatgac
    13801 actgtcgtat cagccggatg gccggaaaac tactttcgcg gcacctgttt tctgcattgc
    13861 ggctgatgat aatccttatg gttttttccc ctcttttttg tacttttatg ttttaatcat
    13921 tttgataatt aaatccatat tgttatgaac gacataagaa acctcgaacc gaaatccctg
    13981 tggagctact tttatgatat aacccggatt ccccgtccct ccaaaaaaga aaaacagatc
    14041 acagaattta ttttgaagtt tgccggacaa tacaagcttg aatcgctggt tgacaaaacc
    14101 gggaatgtga ttctgagaaa aaatgcaaca aaaggtatgg aaaatcgcaa aggtgttatc
    14161 ctccaggcac atctcgacat ggtaccccag aaaaacagcg acaaggttca tgatttcgaa
    14221 aaagatccca ttgaaacaat tattgatggc gaatgggtca gggcaaacgg taccacgctc
    14281 ggatctgaca acgggattgg cgttgccgcg gccatggctg tcctggcatc ggataatatt
    14341 gttcacggcc cgctggaggt gcttttaaca gtcgatgaag agaccggaat gaccggagcc
    14401 ttcggactga aaaaaggcat cctcaggggt gatatacttt taaatctcga ctcggaggat
    14461 gatggcgaat tcaacgtagg atgcgctggc ggaattgatg tgagtgccac cggaagatac
    14521 gggcaagtaa aatgtccggg cggaatgact gcatatcaaa ttatgatcag gggactgaag
    14581 ggcgggcatt caggcgttga tatagccctt ggcagggcaa attcaaacaa gcttttgttc
    14641 cgtttcctga tgcaggctga atcagacttc agaatacgac tcgcagaagc agccgggggt
    14701 gatcttcgca acgctatccc ccgtgaatca caggcaatcg ttcttgttcc gtccgcaagc
    14761 gaagggaaat ttgagaaatt cgtaaaaggt tataataaaa tgtacaagga tgaatttgcc
    14821 ggagttgaac caaccatatc cattacgtgc aaaaagacag aacgccctgc acaggtaatg
    14881 agcatcaagg atcagtacgg gataatccga tcggtatttg cctgccccaa tggtgtgcag
    14941 cggatgagcc ccgccgtgga gggcctggtc gaaacatcaa acaacctggc aattgttaaa
    15001 tgcagcaaag ggaagtatga agtacacaac ctgacaagaa gttctgttga ttcggcaaag
    15061 gaggcaaccg catggaaaat tgccgcaata ttccacctga ttggtgcaaa gatttcatta
    15121 tacggcagtt atccgggatg gaaacccaac atggattccc cgatattaaa aaccatgacc
    15181 aatgtataca ggaaaatgtt cggggaggag ccggaaataa aagctattca tgccggactt
    15241 gaatgcgggc tgataggcgg tatatatccc agacttgata tgatatcatt cgggcctaca
    15301 ataaaatatc cccattcccc tgatgaaaaa gtcctggtac ctacagtcaa aaagtttttt
    15361 gattttcttt gcgaaacctt gaaaaagatt cccgaaaagt aggctctgtc agaaatattt
    15421 caggctgaaa gatctgtttc ccgtgccata caaatcattg tgacaattcc ctgcaatacc
    15481 atagactggc attgcattca gtggtttttt attctatctt gatctctaaa agaggatcat
    15541 ccttcattac gttcgtattg ggtcttgtat taacggcagt aaccctgcca ttgacgggcg
    15601 actgtatttc attctgcatc ttcatggctt caagaattat caccggttcg cccctgagta
    15661 cgctggcacc ttcgcggacc agtacatcta taattttacc aggcatcgga gccttaacca
    15721 gttccttttc agcttttccc ctgcctgagc caaggacttt cagccttttc agggaaaatg
    15781 gagtttcaac ggtaaaggta taacttatgt cgttgaacag tatttcatac ttattctgtc
    15841 tcgatctgac aatttcaact gggtacttcc tgttcttcca taaaatgtag gttccgaact
    15901 tgtcgtctgt cttaagcctg acatcataca ctctcttgcc tattttaata gaatgtttta
    15961 acgggcgcgg gaagcagaat accttatttt cagacgaaag ggcgtaaagc ttgttcaatc
    16021 ctggcttttt gtctgacatt tcaatttaag tattaaaact gattaattct tttattcagc
    16081 aaccatggac tcatttcact ggtatgttca aaatcaaccg gaatactgtc ctcaaggaaa
    16141 ttttcctcga tgaatagttt tgtggccagc caggccgcaa gcatctcttc atattcactg
    16201 ctcaggaaat atttatcgag atccttttca ataaatgcgg tcgtgaaatc gccgttgagg
    16261 aatttctgat tcctgacaag cattctgaaa aacggaaggg ttgttttcgt acccttgatc
    16321 cataccttat ccagtgccat cttgcaattc cggatggcat ccttgcgatc gcggccgctt
    16381 attatgagtt tggctatcat tgaatcgtag ttggcgacga cagatgatcc tgcctgtact
    16441 cctgtgtcaa cccttatata tggtgccgag ggaagactga gattttctat ccttccgggc
    16501 acgggagcaa atcccgactg aacatcttca gcattgatcc tgcattcgat ggcccatcct
    16561 ttaagattta catcattctg ccttattttg agcttatcgc catttgcaac caggatctgc
    16621 tgttcaatga gatcaatgcc ggtaacaatt tcggtcacag gatgctccac ctgtattctt
    16681 gtgttcattt ccatgaaata gaaattcttg tccgaatcaa gaagaaattc aaccgttccg
    16741 gcagaataat aattcaccgc agcagctatt ctgacggcca tattacccat ggttttcctg
    16801 agttcttcat caagtgcaac tgacggagct tcttccagca atttctgatg ctttctctgt
    16861 atggaacagt ccctttcacc aagatggata atattgccgg cagtatcccc gagtatctga
    16921 aattcaatat gtttaggatt ttcaatatat ttctcaatga aaacagaagg atcgttgaat
    16981 gctgtttccg cttccttgct tgcaataatg aacatcctat ccatttcctg gggattcctc
    17041 acaatcctca ttcccctgcc gccacctcct gaagctgcct tgataattat cgggtaacct
    17101 atctttccgg caaccgccat tgcttcatct tcgttgccta tatttccctc acttccgaca
    17161 gccattggaa ttcctgaggc cagcgcaatt ctgcgcgcta ttgttttgtt ccccatttta
    17221 taaatagcat caggtgacgg accgataaaa atgatattgt tatcgatgca tttctgggca
    17281 aaataggcat tctcggcaag gaagccatat cccggatgaa ctgcatcggc gtttgttttt
    17341 cccgcaatct caatgagctt atcgatattc agaaaaaccg gtatgtcaga ggtatcttca
    17401 gtattgtcgt atacaatatc agccattgat acatacattg cgttgggctc tacagctgtc
    17461 ttgatcacaa tcgtactgat cttcatcctg gcagccgttt tgattatcct tacggcaatt
    17521 tctccacggt tggcaataag tattcgttta atcttcatat cagttgattt gatattcatt
    17581 cattcctgat tttagtgtga ggatatttac atgcattttt cccagcccct gccagtcagc
    17641 ttatctgcat taatgattcc aggcctgtta caacggcatg tttccgtgct ttcgtgcagg
    17701 tactttaacc cacttattac tcagggcaga tagggcagat atcactttct tccgggtcac
    17761 ggcaggatca attacttcat cgatatatcc cagctcgtct gcaatgaacg gatttgcaac
    17821 cttgtcgcgg tatttcttta ataattcctt tttgagttca gctggatttt tagattgggc
    17881 aagctctttg cggtaaagaa ttgcaacagc tccgtcggga cccataactg ctatttcagc
    17941 agaaggccat gcataattga aatcgcctcc aaggttttta ctgttcatca ctataaatgc
    18001 acccccgtaa gccttgcgca ggattatggt aattttagg
//
LOCUS       DAOOYG010000048        17738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3080_length_17738_cov_7.219476, whole genome shotgun sequence.
ACCESSION   DAOOYG010000048 DAOOYG010000000
VERSION     DAOOYG010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17738
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3080_length_17738_cov_7.219476"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PK288_05930"
     CDS             complement(<1..366)
                     /locus_tag="PK288_05930"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338270.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPM18493.1"
                     /translation="MKKSTKISLSEKEIPRQWYNIVADMPNKPLPPLHPVTREPIGPD
                     ALAAVFPMELIKQEVSAERYIDIPDEVLDLYKVYRPSPMFRAVNLEKAIGTRSKIYYK
                     YEGGNYSGSHKTNTAIAQAY"
     gene            complement(639..1889)
                     /gene="aroA"
                     /locus_tag="PK288_05935"
     CDS             complement(639..1889)
                     /gene="aroA"
                     /locus_tag="PK288_05935"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPM18494.1"
                     /translation="MVRSVDPSFIAGEIKSPSSKSMTQRAIAAAMLAKGSSIIKNPSY
                     CNDSLAAMSIARGFGAKIVPEESLMYVTGRPGVTDNTLNCGESGLAIRMFSPIAALFP
                     EKIVMLGKGSLRNRPMQMIGDALIQLGARFESTGGFLPLTIQGPLKGGRCEIDGSVSS
                     QLLTGLLMALPLTAEDSEIFVTNLKSKPYVDMTMKVLRDFGITVSHEDYKLFTIRGRQ
                     AYSPVNIEVEGDWSGGAFLLVAGAINGNVVVRGLQAGSCQSDRAILEAMEKAGAMITI
                     SQDWISVTKSDMKAFEFDATDAPDLFPPLAALAAYCNGISRIKGASRLIHKESNRAAA
                     LISEFGKMNIEIRLEDDLMLITGGRTMGAAVSSHNDHRIAMAAAVAALGAEGRVSIAD
                     SQCVAKSYPDFFNDLMKAGVSVIE"
     gene            complement(1902..3020)
                     /locus_tag="PK288_05940"
     CDS             complement(1902..3020)
                     /locus_tag="PK288_05940"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPM18495.1"
                     /translation="MVLETGTTHIRDWRGYKGRPFLISGPCSAETEEQVMATAKELAQ
                     LKEVNLFRAGIWKPRTRPNSFEGIGIEGLKWLRNVKQETGLMVGTEVANEKHVYEALK
                     YGVDMLWIGARTSVNPFTVQEISNALNGVDVMVLVKNPINPDLDLWIGAIERIARAGI
                     SRLGAIHRGFSTYEKTVYRNQPNWQLPIELRRRMPDLPILCDPSHIAGARRYLYEIAQ
                     KALDLNFDGLMIESHIDPDKALSDSAQQVTPNDLRELLSRLILRSPVPSDPKLLDILG
                     ELRQQIDIFDDYLLDILEKRMKVAETIGQYKKENNITILQSTRWDEIISKVMINGESK
                     GLSRELIDTVFKAIHQESINHQMRVMNNGVMNLAVDKH"
     gene            complement(3032..3877)
                     /locus_tag="PK288_05945"
     CDS             complement(3032..3877)
                     /locus_tag="PK288_05945"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPM18496.1"
                     /translation="MTIAVVGIGLIGGSLMIDLRKRGFAQKIIGVDLNMQHRNIAQLC
                     GIVDETDSLENAVDRSDLVILSSPVDANCMMLPSILDRIQGTGKVVSDMGSTKASIAR
                     VSEHHPGRGRFVALHPMAGTEFSGPLAAIGKLFDYKIAIICDAEKSDVDARRIVESML
                     DTLNMRRIYMDSADHDIHVAYVSHISHITSFSLALSVLDKEKEEEHITTLAGGGFEST
                     VRLAKSSAETWAPIFLENSEFILEVMDNYIEKMNLFKKMIRERNKEGLMALMEEANKI
                     RKILH"
     gene            complement(3874..5064)
                     /locus_tag="PK288_05950"
     CDS             complement(3874..5064)
                     /locus_tag="PK288_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM18497.1"
                     /translation="MKVEPADRTLLVEEYYFSRKLKQIEEMRKGGADVINLGIGSPDQ
                     PPSAGTIERLTAEAAKPNVHGYQSYTGIPALRKAFADWYMRYFGIRLDPEGEILPLIG
                     SKEGIMHITMAFVNPGDEVLVPDPGYPTYSSATSLAGGRVRYYDLTEENRWLPDLDLL
                     EKSDLSRTKIMWINYPHMPTGAKASAGKLSEMVSFASSHGILLCNDNPYSFILNTEYM
                     SVLSAENAMDTAIELNSLSKSHNMAGWRIGMAAGNREYINTILKVKSNMDSGMFMPMQ
                     MAAVAALSNPDSWYEGLNSLYLSRRKIAESILEILGCTFDTDQAGLFLWGRIPDDAVS
                     AEDLSEKILTEAHVFITPGFIFGKNGRRYIRISLCAGEDRLSEARDRLTSAKHGHSHS
                     TLLS"
     gene            complement(5054..5887)
                     /locus_tag="PK288_05955"
     CDS             complement(5054..5887)
                     /locus_tag="PK288_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPM18498.1"
                     /translation="MKVSMQGEPGCFHEVAARQYFGNDAVIDMIPCSTFDQTLKVVQE
                     GYADLAMMAIENARSGSILYNYNLIRESGLKMLGEHNLRIVQNLMALPGQSINDIKQI
                     RSHPIALSQCMTFLNNYPNITLVEASDTAGSAKLISEHKLKKIAAIASSKAAEMYGLE
                     IIAPGIETYKKNYTRFLVIGNEQMGKKDGNKMSVCFATGHKPGSLATVLVRLAQMNIN
                     LSKIQSVPRLNGGWEYMFYLDLELPSGISLDTIEEILYKDTSNLEILGIYNKGEMIHE
                     S"
     gene            complement(6321..6974)
                     /gene="hisIE"
                     /locus_tag="PK288_05960"
     CDS             complement(6321..6974)
                     /gene="hisIE"
                     /locus_tag="PK288_05960"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HPM18499.1"
                     /translation="MKTRYRSTEKQAMESIDFSKGNGLVPAVIQDYNTLQVLMVGFMN
                     EEALKKTVAEGRVTFFSRSKNRLWTKGETSGNYLYVREITADCDQDSLLVRVNPAGPV
                     CHTGNKSCFGSEVPKGFIYELEKIVNQRIDDNAGNSYTNKIFRQGINRMAQKVGEEAI
                     ELIIESKDDNNNLFINEAADLLYHLLILLKAKKLSFSDVEKVLLSRHKPAKNSDSIS"
     gene            complement(7051..7806)
                     /gene="hisF"
                     /locus_tag="PK288_05965"
     CDS             complement(7051..7806)
                     /gene="hisF"
                     /locus_tag="PK288_05965"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPM18500.1"
                     /translation="MMKKRIIPCLDIKDGRTVKGVNFVRFRDAGDPVELAREYVKQQA
                     DELVFLDITATVEKRKTITELVSRIAGEISIPFTVGGGINSAEDVELLLAAGADKVSI
                     NTAAVRNPGLVEEIAGKFGSQCLVVAIDTDRINGNWNVIVDGGQTPTTLGTVEWAVKV
                     MKLGAGEILLTSMDADGTKSGFSIEITRMVTEAVKIPVIASGGAGNMDHFVDVFSQTG
                     CSAALAAGIFHFGEVSIPELKKYLAARNIQVRI"
     gene            complement(8072..8785)
                     /locus_tag="PK288_05970"
     CDS             complement(8072..8785)
                     /locus_tag="PK288_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HPM18501.1"
                     /translation="MRIIIAMDIIDGKCVRLTRGDYSTRRIYGSDPLEVARQAEDHGF
                     SYLHLVDLDGAREKKIINHNILRKIASATSLKIDFGGGIRTTDDLRLLFESGANQITA
                     GSISVTNEALFMNWIEIFGPEKIILGADSRNRLISSGAWMEDSDIEVIEFLTGYAARG
                     VKYALCTDIEKDGMLNGPSVSLYREILEIPGINLIASGGISTIEDVKMLSDMGCEGAV
                     IGKALYEGLMDLNELSKLL"
     gene            8954..9667
                     /locus_tag="PK288_05975"
     CDS             8954..9667
                     /locus_tag="PK288_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HPM18502.1"
                     /translation="MRIIIAMDIIDGKCVRLTRGDYSTRRIYGSDPLEVARQAEDHGF
                     SYLHLVDLDGAREKKIINHNILRKIASATSLKIDFGGGIRTTDDLRLLFESGANQITA
                     GSISVTNEALFMNWIEIFGPEKIILGADSRNRLISSGAWMEDSDIEVIEFLTGYAARG
                     VKYALCTDIEKDGMLNGPSVSLYREILEIPGINLIASGGISTIEDVKMLSDMGCEGAV
                     IGKALYEGLMDLNELSKLL"
     gene            9933..10688
                     /gene="hisF"
                     /locus_tag="PK288_05980"
     CDS             9933..10688
                     /gene="hisF"
                     /locus_tag="PK288_05980"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPM18503.1"
                     /translation="MMKKRIIPCLDIKDGRTVKGVNFVRFRDAGDPVELAREYVKQQA
                     DELVFLDITATVEKRKTITELVSRIAGEISIPFTVGGGINSAEDVELLLAAGADKVSI
                     NTAAVRNPGLVEEIAGKFGSQCLVVAIDTDRINGNWNVIVDGGQTPTTLGTVEWAVKV
                     MKLGAGEILLTSMDADGTKSGFSIEITRMVTEAVKIPVIASGGAGNMDHFVDVFSQTG
                     CSAALAAGIFHFGEVSIPELKKYLAARNIQVRI"
     gene            10765..11418
                     /gene="hisIE"
                     /locus_tag="PK288_05985"
     CDS             10765..11418
                     /gene="hisIE"
                     /locus_tag="PK288_05985"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HPM18504.1"
                     /translation="MKTRYRSTEKQAMESIDFSKGNGLVPAVIQDYNTLQVLMVGFMN
                     EEALKKTVAEGRVTFFSRSKNRLWTKGETSGNYLYVREITADCDQDSLLVRVNPAGPV
                     CHTGNKSCFGSEVPKGFIYELEKIVNQRIDDNAGNSYTNKIFRQGINRMAQKVGEEAI
                     ELIIESKDDNNNLFINEAADLLYHLLILLKAKKLSFSDVEKVLLSRHKPAKNSDSIS"
     gene            11852..12685
                     /locus_tag="PK288_05990"
     CDS             11852..12685
                     /locus_tag="PK288_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPM18505.1"
                     /translation="MKVSMQGEPGCFHEVAARQYFGNDAVIDMIPCSTFDQTLKVVQE
                     GYADLAMMAIENARSGSILYNYNLIRESGLKMLGEHNLRIVQNLMALPGQSINDIKQI
                     RSHPIALSQCMTFLNNYPNITLVEASDTAGSAKLISEHKLKKIAAIASSKAAEMYGLE
                     IIAPGIETYKKNYTRFLVIGNEQMGKKDGNKMSVCFATGHKPGSLATVLVRLAQMNIN
                     LSKIQSVPRLNGGWEYMFYLDLELPSGISLDTIEEILYKDTSNLEILGIYNKGEMIHE
                     S"
     gene            12675..13865
                     /locus_tag="PK288_05995"
     CDS             12675..13865
                     /locus_tag="PK288_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPM18506.1"
                     /translation="MKVEPADRTLLVEEYYFSRKLKQIEEMRKGGADVINLGIGSPDQ
                     PPSAGTIERLTAEAAKPNVHGYQSYTGIPALRKAFADWYMRYFGIRLDPEGEILPLIG
                     SKEGIMHITMAFVNPGDEVLVPDPGYPTYSSATSLAGGRVRYYDLTEENRWLPDLDLL
                     EKSDLSRTKIMWINYPHMPTGAKASAGKLSEMVSFASSHGILLCNDNPYSFILNTEYM
                     SVLSAENAMDTAIELNSLSKSHNMAGWRIGMAAGNREYINTILKVKSNMDSGMFMPMQ
                     MAAVAALSNPDSWYEGLNSLYLSRRKIAESILEILGCTFDTDQAGLFLWGRIPDDAVS
                     AEDLSEKILTEAHVFITPGFIFGKNGRRYIRISLCAGEDRLSEARDRLTSAKHGHSHS
                     TLLS"
     gene            13862..14707
                     /locus_tag="PK288_06000"
     CDS             13862..14707
                     /locus_tag="PK288_06000"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPM18507.1"
                     /translation="MTIAVVGIGLIGGSLMIDLRKRGFAQKIIGVDLNMQHRNIAQLC
                     GIVDETDSLENAVDRSDLVILSSPVDANCMMLPSILDRIQGTGKVVSDMGSTKASIAR
                     VSEHHPGRGRFVALHPMAGTEFSGPLAAIGKLFDYKIAIICDAEKSDVDARRIVESML
                     DTLNMRRIYMDSADHDIHVAYVSHISHITSFSLALSVLDKEKEEEHITTLAGGGFEST
                     VRLAKSSAETWAPIFLENSEFILEVMDNYIEKMNLFKKMIRERNKEGLMALMEEANKI
                     RKILH"
     gene            14719..15837
                     /locus_tag="PK288_06005"
     CDS             14719..15837
                     /locus_tag="PK288_06005"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPM18508.1"
                     /translation="MVLETGTTHIRDWRGYKGRPFLISGPCSAETEEQVMATAKELAQ
                     LKEVNLFRAGIWKPRTRPNSFEGIGIEGLKWLRNVKQETGLMVGTEVANEKHVYEALK
                     YGVDMLWIGARTSVNPFTVQEISNALNGVDVMVLVKNPINPDLDLWIGAIERIARAGI
                     SRLGAIHRGFSTYEKTVYRNQPNWQLPIELRRRMPDLPILCDPSHIAGARRYLYEIAQ
                     KALDLNFDGLMIESHIDPDKALSDSAQQVTPNDLRELLSRLILRSPVPSDPKLLDILG
                     ELRQQIDIFDDYLLDILEKRMKVAETIGQYKKENNITILQSTRWDEIISKVMINGESK
                     GLSRELIDTVFKAIHQESINHQMRVMNNGVMNLAVDKH"
     gene            15850..17100
                     /gene="aroA"
                     /locus_tag="PK288_06010"
     CDS             15850..17100
                     /gene="aroA"
                     /locus_tag="PK288_06010"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPM18509.1"
                     /translation="MVRSVDPSFIAGEIKSPSSKSMTQRAIAAAMLAKGSSIIKNPSY
                     CNDSLAAMSIARGFGAKIVPEESLMYVTGRPGVTDNTLNCGESGLAIRMFSPIAALFP
                     EKIVMLGKGSLRNRPMQMIGDALIQLGARFESTGGFLPLTIQGPLKGGRCEIDGSVSS
                     QLLTGLLMALPLTAEDSEIFVTNLKSKPYVDMTMKVLRDFGITVSHEDYKLFTIRGRQ
                     AYSPVNIEVEGDWSGGAFLLVAGAINGNVVVRGLQAGSCQSDRAILEAMEKAGAMITI
                     SQDWISVTKSDMKAFEFDATDAPDLFPPLAALAAYCNGISRIKGASRLIHKESNRAAA
                     LISEFGKMNIEIRLEDDLMLITGGRTMGAAVSSHNDHRIAMAAAVAALGAEGRVSIAD
                     SQCVAKSYPDFFNDLMKAGVSVIE"
     gene            17373..>17738
                     /locus_tag="PK288_06015"
     CDS             17373..>17738
                     /locus_tag="PK288_06015"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338270.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPM18510.1"
                     /translation="MKKSTKISLSEKEIPRQWYNIVADMPNKPLPPLHPVTREPIGPD
                     ALAAVFPMELIKQEVSAERYIDIPDEVLDLYKVYRPSPMFRAVNLEKAIGTRSKIYYK
                     YEGGNYSGSHKTNTAIAQAY"
BASE COUNT         4774 a         4095 c         4095 g         4774 t
ORIGIN      
        1 ataggcctgt gcaatggcag tgttggtctt atgggaaccg gagtagttgc ctccctcgta
       61 cttgtagtat atcttgctcc tggttcctat tgccttttcc agattgaccg ccctgaacat
      121 cggacttggc cggtatacct tgtacagatc caggacttcg tcgggtatat caatataccg
      181 ttctgcagaa acctcctgtt tgatcagttc cattggaaaa accgcggcaa gtgcatcggg
      241 tccaatgggc tcgcgtgtca ccggatgaag aggcggcagc ggtttattgg gcatatctgc
      301 cacaatatta taccattgcc gggggatttc tttttcactg agcgaaattt ttgtactttt
      361 tttcatgact ttatttattt tggttaatga gttttaaata aaaaaggcat gctgtgaaca
      421 gcatgccttt ggcgggaaaa tatctttgcg aagattatat acaggcctta ttcacagctt
      481 gctgattgat gctgcgccaa gaccagatat ataacattcc atggttcatg gctccaaaat
      541 tatttaattt ttttaaattc caataaaaaa gttctttttt ttaatgattt tcagaaaaaa
      601 tatatctgac cgggaaatct tatagctgcc tgcaattatt attcaataac agaaacacct
      661 gctttcatga ggtcattgaa gaaatccgga tacgattttg ccacacattg cgaatcagct
      721 attgacaccc tgccttcggc ccctagtgca gcaacagcgg ctgccattgc gatccggtgg
      781 tcgttatgcg atgatactgc ggctcccatt gtcctgcctc cggtaatcag catcaggtca
      841 tcctcgagtc ttatttcaat gttcatttta ccgaattcgc ttataagggc tgctgccctg
      901 ttgctttcct tgtggatgag ccgcgatgca cccttaatcc ttgaaatgcc gttgcagtag
      961 gctgccaggg cggcaagagg gggaaacagg tcgggcgcgt cggtggcgtc gaactcgaaa
     1021 gctttcatat cggatttggt tactgaaatc cagtcctggc ttattgtgat cattgctccg
     1081 gctttttcca tggcttccag gattgccctg tcgctctggc aacttcctgc ctgaaggccc
     1141 ctgacaacca cgttgccgtt tattgctccc gcaacgagca ggaaagctcc gccgctccag
     1201 tcgccctcta cttcaatgtt taccggggaa taggcctgcc tgcccctgat agtgaacagt
     1261 ttatagtctt catggcttac cgttatgcca aaatccctga gaactttcat tgtcatgtca
     1321 acatacggct tgcttttaag gttggtcacg aagatctcgg aatcttcagc ggtaagcggc
     1381 agggccatta ggagccctgt aagaagctgg gaactcaccg atccgtcaat ttcgcaccgt
     1441 ccgcctttta acggcccctg aattgtaagt ggaagaaaac cgccggtact ttcaaacctg
     1501 gctcccagct ggattaatgc atcccctatc atttgcatcg gcctgtttct gagtgagcct
     1561 tttccaagca tgacgatctt ttcgggaaac aatgccgcaa tcggagagaa catcctgatg
     1621 gcaagacctg actctccgca attgagagta ttgtccgtca ccccgggccg gcctgtgaca
     1681 tacatcagac tctcttccgg cactattttt gctccaaaac ccctggcaat actcatagca
     1741 gcgagagagt cattgcaata cgaaggattc tttattatac tgcttccttt tgcaagcatt
     1801 gctgctgcaa tggctctctg tgtcatgctc ttggaagatg gtgattttat ctcccctgcg
     1861 ataaaggatg gatcaacaga tcgaaccata tcactgcaaa attaatgttt atcaacggca
     1921 agattcatga caccattatt cattactctc atctggtggt tgatcgattc ctggtgaatg
     1981 gccttaaaga cggtatctat cagttccctg ctgagtccct tggactctcc gttgatcatg
     2041 accttgctga tgatctcatc ccagcgggtt gactgcagga tagtgatatt gttttccttt
     2101 ttgtactggc cgattgtttc tgcgactttc attcgcttct ccagaatatc gaggaggtag
     2161 tcgtcgaaaa tatcaatctg ctgccttaat tctccgagaa tatcgagcag tttcggatcg
     2221 gaaggtaccg ggcttctcag gatcagcctg ctgagaagtt ccctgagatc attgggggta
     2281 acctgctgtg ctgaatcact gagagcttta tcgggatcga tatgagattc tatcattaaa
     2341 ccatcaaaat tcaggtcgag ggctttctgt gcgatttcat acaggtatct cctggccccg
     2401 gcaatgtgac ttgggtcaca cagtattggc agatccggca tgcgtcttcg cagctcaatc
     2461 ggcagctgcc agttgggctg attacggtaa actgtttttt cgtatgttga aaatccgcga
     2521 tgaattgccc cgagcctgct tatccctgcc cttgcaatac gttcgatggc accgatccac
     2581 agatcaagat cggggttgat ggggttcttt accagtacca tgacatccac accgttgagt
     2641 gcatttgaaa tttcctgtac ggtgaaagga ttgacagaag tcctggcgcc tatccacagc
     2701 atgtcaacgc catatttaag tgcttcataa acgtgttttt cgttggcaac ttccgtaccg
     2761 accatcaggc ctgtttcctg tttcacattt ctcagccatt tgagtccttc aatacctatt
     2821 ccctcaaatg aattcggcct ggttcggggt ttccagatgc ctgccctgaa aaggttgact
     2881 tctttgagct gggccagttc ctttgcagtt gccataacct gttcctcggt ttcagcgctg
     2941 caggggcccg atatcaggaa aggtctgccc ttgtagcctc tccagtccct gatgtgtgtt
     3001 gttcccgttt caagtaccat gttattgttt tttagtgtaa tattttcctg atcttgtttg
     3061 cctcttccat taatgccatc aggccctctt tattcctttc ccttatcatt ttcttgaaca
     3121 gattcatttt ttcgatataa ttatccatga cttccaatat gaattcggag ttttccagga
     3181 aaatgggagc ccaggtttcg gcactgctct ttgccagcct gacggtgctt tcaaaaccgc
     3241 cgcctgcaag ggttgtaatg tgctcttcct ccttctcctt gtccagcacg ctcagggcca
     3301 gggagaaaga agtgatgtgg gaaatatgcg atacataggc cacatgtatg tcatgatcag
     3361 cagaatccat gtatatcctc ctcatgttaa gagtgtcgag catactttcg actatccttc
     3421 ttgcatcaac atcgcttttt tcggcatcgc agattattgc gatcttgtaa tcaaacagct
     3481 tgccaatggc cgcaagcggt ccggaaaact ctgttccggc cattgggtgc agggcaacga
     3541 atcgccccct gcccgggtga tgttccgata ccctggcaat acttgccttg gtcgatccca
     3601 tgtcgctaac caccttgccg gtaccctgaa ttctgtcgag gattgacggg agcatcatgc
     3661 aattggcatc gaccgggctg cttaaaatga ccaggtcgct cctgtcaacg gcattttcaa
     3721 gcgaatcggt ttcatccaca atgccgcaga gctgggctat gttccggtgc tgcatattta
     3781 aatccacgcc aattattttc tgggcaaacc ctcgcttgcg aagatcgatc atcaatgatc
     3841 ctccgatgag ccctattcct acaacagcta ttgtcatgag agtaaagttg aatgcgaatg
     3901 accgtgtttt gcagatgtca gcctgtcccg ggcttctgaa agccggtcct cgccggcaca
     3961 tagtgaaata cggatatatc tccgtccgtt ctttccgaaa ataaatcccg gagtaataaa
     4021 aacatgcgct tctgtcagta ttttttctga aagatcttcg gcgcttacag cgtcgtccgg
     4081 tattctgccc cagaggaaca accctgcctg gtcagtgtcg aaagtgcaac ccaggatttc
     4141 aaggattgat tctgctattt tccggcgact gagatacaga ctgttcaggc cttcatacca
     4201 tgaatccgga ttactgagcg cagcaacggc agccatttgc attggcatga acatccctga
     4261 gtccatgttg cttttcactt tcagaatagt gtttatatac tccctgttcc ctgctgccat
     4321 tccaatcctc catccggcca tattatggga tttactcagg gagtttagct cgattgccgt
     4381 gtccattgca ttttcggcac tcaggacgct catgtattcg gtgttcagga tgaaactgta
     4441 tggattgtcg ttgcataaca ggataccatg gctggaagca aatgaaacca tctccgataa
     4501 ttttccagct gaggcttttg ctccggttgg catgtgagga tagtttatcc acatgatttt
     4561 ggtcctggac agatcgcttt tttcaagcag atcaagatca ggaagccacc tgttttcctc
     4621 tgtgagatca tagtacctga ccctgccgcc cgcgagactc gttgccgatg agtaggttgg
     4681 atatccggga tcaggcacca gaacttcatc accggggttt acaaaggcca tggtgatatg
     4741 cataatgcct tccttgctgc caatgagcgg caggatctca ccttccggat caagccttat
     4801 cccgaagtac ctcatgtacc agtcggcaaa tgcttttctg agtgcaggga taccagtgta
     4861 actctgataa ccatgaacat tgggttttgc agcctcagca gtaaggcgct caatcgtgcc
     4921 ggctgacgga ggctgatcgg ggctgccgat accaagattt ataacatcgg cccctccctt
     4981 gcgcatttcc tcaatctgct tcagtttgcg ggagaagtaa tattcctcga ccagtaaggt
     5041 cctgtctgca ggttcaactt tcatggatca tttctccttt attgtaaata cccagtattt
     5101 ccagattgct ggtatccttg taaagaattt cctcaatagt atcaagcgag attcccgaag
     5161 gaagctccag atcgagatag aacatgtatt cccatcctcc gttgagccgt ggtaccgact
     5221 gtatttttga aaggttgatg ttcatttgtg cgagcctgac gagtactgtt gcaagtgacc
     5281 cgggcttatg gcctgtggca aagcagactg acatcttgtt gccgtccttt ttccccattt
     5341 gctcattacc gataaccagg aatcgtgtat aattcttttt ataggtttca atgccgggag
     5401 caattatctc aagaccgtac atctcggctg cctttgaaga agcaatggct gctatctttt
     5461 taagcttgtg ttccgagatt agttttgcac ttcctgcagt atcacttgcc tcaacgagtg
     5521 ttatattcgg gtaattgttc aggaaggtca tgcattgaga caatgctatc ggatgcgacc
     5581 tgatctgctt aatgtcatta attgactgtc cgggcaaagc catcagattc tggactatcc
     5641 tgagattgtg ctctccaagc attttcagcc ccgattcccg gataaggttg tagttgtaca
     5701 aaatgcttcc ggagcgggca ttctcaattg ccatcatggc caggtcagcg taaccttcct
     5761 gcacgacttt aagcgtctgg tcaaaagttg aacaaggaat catgtcaatg acggcatcgt
     5821 tgccgaaata ctgtcttgcc gctacttcgt gaaaacatcc gggttctccc tgcattgata
     5881 ctttcatttc ctatggtatt aaaattaaga agggtccctt ttggggaccc ttgctttcat
     5941 attgtctttt tatacgtata caaaaatccc cttatctctt tttgaaataa aaaaagaaat
     6001 aaaagaagaa atagttgtat acgaaattca ttgtcatttg ctttactggg tgacaaaatt
     6061 aactcataaa acaataattc caaaaaaaat aaataaaaat aaagaattac tgtaattttt
     6121 tatgttttga gcgggatatc gataagagtg agtctcacat ggagcatctt ttcttttatt
     6181 ggtttgatgg cattttcttt ttcttcacca taagaaagtg acatcaaaaa gggaggttcc
     6241 gcgccgccaa atccaggatt tgctgttgcg aatcatgtaa aatgcgcaat aaccaggctg
     6301 aaataaatta ataactgctg tcaggatata gaatctgaat ttttagcggg tttgtgtctt
     6361 gaaagaagta ctttttccac gtcggaaaac gacagttttt tggctttaag cagtatcagc
     6421 agatggtaaa gcagatcagc tgcttcgtta atgaacaggt tattgttatc atcttttgat
     6481 tctatgatca gttcaatggc ttcttcaccc actttctggg ccatcctgtt tattccctgc
     6541 ctgaagatct tgtttgtgta ggaattcccg gcattatcgt caatcctttg atttacgatt
     6601 ttttcaagct cgtatatgaa tcccttggga acttcactgc caaaacaaga cttgtttccc
     6661 gtgtgacaca cagggccggc gggattgacc ctgacaagca atgaatcctg gtcacaatct
     6721 gctgttattt ctctaacata caaataattg ccggaagttt ccccttttgt ccacaaacgg
     6781 tttttgcttc tgctgaagaa tgtaaccctg ccttcagcca ctgtcttttt caatgcttcc
     6841 tcattcatga accctaccat taaaacctgg agggtgttgt agtcctgaat gactgctggg
     6901 accagtccgt ttcctttact gaagtcaatt gattccatag cctgtttttc tgttgatctg
     6961 tatcttgttt ttatttattt ctgattatct gtgaaatgcc cggttgctta ttggatttcc
     7021 gcaattgcta ccgggggcgg aacgggtgta ttatattctt acctggatat ttctggcggc
     7081 aagatatttt ttcaattcag ggatggaaac ttccccgaaa tgaaatatac ctgccgccaa
     7141 tgcggcacta cagccggttt gactgaagac atccacgaaa tgatccatat ttccagctcc
     7201 gccggaggcg ataaccggta tctttacagc ttcagttacc attctggtaa tttcgatgga
     7261 aaatcccgat tttgtaccgt cggcatccat cgaggtcaga aggatctccc cggctcccag
     7321 tttcatgacc ttcactgccc actcaactgt tcccagggtt gtcggagtct ggccgccatc
     7381 gacaataacg ttccagtttc cgtttatgcg gtcagtgtct attgcaacca caaggcactg
     7441 gctcccgaat ttccctgcaa tttcttctac caggcccggg ttcctgactg cagctgtgtt
     7501 tatgcttact ttatctgcac cggcggcaag gagtaactca acgtcttctg cagagtttat
     7561 gcctccgccg acagtaaatg gaatgcttat ttctcctgca atccggctta ccagttccgt
     7621 aatggtcttc cgtttttcca cggtggcggt gatatcgagg aaaacgagtt catcggcttg
     7681 ctgttttaca tattccctgg caagttcaac aggatcgcct gcatcccgga acctcacgaa
     7741 attaaccccc ttgactgtcc ttccgtcttt aatgtcgagg catggaatga ttcttttttt
     7801 cattataata taattttgat tttaatggta tgatgcccgc ctgacccgtc gggcagggaa
     7861 ccgcacatga ttgactttgt cgagctcgca agcccggttg aaaattacat tcttatcgtg
     7921 tttgtgattg tggtcaatca tgtgggtttc atctgtatat catttatgtt ttaaagtccg
     7981 gatgcctgtc tgagccccgg gcaggggacc acacatgacc ggatgagttt atatccatcc
     8041 gggtttatgt ttttggttaa ccttgagggt ttcatagcag tttgctaagt tcatttaaat
     8101 ccattaaacc ttcataaagt gcttttccaa ttacagctcc ttcacatccc atgtccgaaa
     8161 gcattttcac atcttcaatt gtcgaaatac caccactggc aataaggttt attccgggga
     8221 tctccaggat ttccctgtaa aggcttactg aaggaccgtt aagcatacca tccttttcaa
     8281 tgtcggtgca gagggcatac ttcacgccac gtgcggcata gccggttaaa aattcaatga
     8341 cctcaatatc tgaatcctcc atccatgctc ctgatgatat cagcctgttc ctcgaatcag
     8401 cacccaggat tattttttcc ggtccgaaaa tttcaatcca gttcatgaag agtgcttcat
     8461 tggttactga aatacttcca gcggtaatct gatttgcccc ggattcaaaa agaagcctga
     8521 ggtcgtcggt agttctgatg cctcctccga aatcgatctt cagggatgtg gcggatgcga
     8581 tcttcctgag aatattgtga ttgatgatct ttttctccct ggcgccatcc agatcgacca
     8641 ggtgcaggta actgaagccg tgatcctctg cctgccttgc cacttcaagg gggtccgaac
     8701 cgtaaatccg gcgtgtcgag taatcgcccc tggtgagcct cacacatttg ccgtcaataa
     8761 tatccattgc aataattatc ctcatagttc gggtagatta taaaatatcc gtatgcccgg
     8821 ctccgggtat gatcattttc ccggcagaaa taattccccc gggatcgccg gcgatcccgg
     8881 gggaattatt tctgccggga aaatgatcat acccggagcc gggcatacgg atattttata
     8941 atctacccga actatgagga taattattgc aatggatatt attgacggca aatgtgtgag
     9001 gctcaccagg ggcgattact cgacacgccg gatttacggt tcggaccccc ttgaagtggc
     9061 aaggcaggca gaggatcacg gcttcagtta cctgcacctg gtcgatctgg atggcgccag
     9121 ggagaaaaag atcatcaatc acaatattct caggaagatc gcatccgcca catccctgaa
     9181 gatcgatttc ggaggaggca tcagaactac cgacgacctc aggcttcttt ttgaatccgg
     9241 ggcaaatcag attaccgctg gaagtatttc agtaaccaat gaagcactct tcatgaactg
     9301 gattgaaatt ttcggaccgg aaaaaataat cctgggtgct gattcgagga acaggctgat
     9361 atcatcagga gcatggatgg aggattcaga tattgaggtc attgaatttt taaccggcta
     9421 tgccgcacgt ggcgtgaagt atgccctctg caccgacatt gaaaaggatg gtatgcttaa
     9481 cggtccttca gtaagccttt acagggaaat cctggagatc cccggaataa accttattgc
     9541 cagtggtggt atttcgacaa ttgaagatgt gaaaatgctt tcggacatgg gatgtgaagg
     9601 agctgtaatt ggaaaagcac tttatgaagg tttaatggat ttaaatgaac ttagcaaact
     9661 gctatgaaac cctcaaggtt aaccaaaaac ataaacccgg atggatataa actcatccgg
     9721 tcatgtgtgg tcccctgccc ggggctcaga caggcatccg gactttaaaa cataaatgat
     9781 atacagatga aacccacatg attgaccaca atcacaaaca cgataagaat gtaattttca
     9841 accgggcttg cgagctcgac aaagtcaatc atgtgcggtt ccctgcccga cgggtcaggc
     9901 gggcatcata ccattaaaat caaaattata ttataatgaa aaaaagaatc attccatgcc
     9961 tcgacattaa agacggaagg acagtcaagg gggttaattt cgtgaggttc cgggatgcag
    10021 gcgatcctgt tgaacttgcc agggaatatg taaaacagca agccgatgaa ctcgttttcc
    10081 tcgatatcac cgccaccgtg gaaaaacgga agaccattac ggaactggta agccggattg
    10141 caggagaaat aagcattcca tttactgtcg gcggaggcat aaactctgca gaagacgttg
    10201 agttactcct tgccgccggt gcagataaag taagcataaa cacagctgca gtcaggaacc
    10261 cgggcctggt agaagaaatt gcagggaaat tcgggagcca gtgccttgtg gttgcaatag
    10321 acactgaccg cataaacgga aactggaacg ttattgtcga tggcggccag actccgacaa
    10381 ccctgggaac agttgagtgg gcagtgaagg tcatgaaact gggagccggg gagatccttc
    10441 tgacctcgat ggatgccgac ggtacaaaat cgggattttc catcgaaatt accagaatgg
    10501 taactgaagc tgtaaagata ccggttatcg cctccggcgg agctggaaat atggatcatt
    10561 tcgtggatgt cttcagtcaa accggctgta gtgccgcatt ggcggcaggt atatttcatt
    10621 tcggggaagt ttccatccct gaattgaaaa aatatcttgc cgccagaaat atccaggtaa
    10681 gaatataata cacccgttcc gcccccggta gcaattgcgg aaatccaata agcaaccggg
    10741 catttcacag ataatcagaa ataaataaaa acaagataca gatcaacaga aaaacaggct
    10801 atggaatcaa ttgacttcag taaaggaaac ggactggtcc cagcagtcat tcaggactac
    10861 aacaccctcc aggttttaat ggtagggttc atgaatgagg aagcattgaa aaagacagtg
    10921 gctgaaggca gggttacatt cttcagcaga agcaaaaacc gtttgtggac aaaaggggaa
    10981 acttccggca attatttgta tgttagagaa ataacagcag attgtgacca ggattcattg
    11041 cttgtcaggg tcaatcccgc cggccctgtg tgtcacacgg gaaacaagtc ttgttttggc
    11101 agtgaagttc ccaagggatt catatacgag cttgaaaaaa tcgtaaatca aaggattgac
    11161 gataatgccg ggaattccta cacaaacaag atcttcaggc agggaataaa caggatggcc
    11221 cagaaagtgg gtgaagaagc cattgaactg atcatagaat caaaagatga taacaataac
    11281 ctgttcatta acgaagcagc tgatctgctt taccatctgc tgatactgct taaagccaaa
    11341 aaactgtcgt tttccgacgt ggaaaaagta cttctttcaa gacacaaacc cgctaaaaat
    11401 tcagattcta tatcctgaca gcagttatta atttatttca gcctggttat tgcgcatttt
    11461 acatgattcg caacagcaaa tcctggattt ggcggcgcgg aacctccctt tttgatgtca
    11521 ctttcttatg gtgaagaaaa agaaaatgcc atcaaaccaa taaaagaaaa gatgctccat
    11581 gtgagactca ctcttatcga tatcccgctc aaaacataaa aaattacagt aattctttat
    11641 ttttatttat tttttttgga attattgttt tatgagttaa ttttgtcacc cagtaaagca
    11701 aatgacaatg aatttcgtat acaactattt cttcttttat ttcttttttt atttcaaaaa
    11761 gagataaggg gatttttgta tacgtataaa aagacaatat gaaagcaagg gtccccaaaa
    11821 gggacccttc ttaattttaa taccatagga aatgaaagta tcaatgcagg gagaacccgg
    11881 atgttttcac gaagtagcgg caagacagta tttcggcaac gatgccgtca ttgacatgat
    11941 tccttgttca acttttgacc agacgcttaa agtcgtgcag gaaggttacg ctgacctggc
    12001 catgatggca attgagaatg cccgctccgg aagcattttg tacaactaca accttatccg
    12061 ggaatcgggg ctgaaaatgc ttggagagca caatctcagg atagtccaga atctgatggc
    12121 tttgcccgga cagtcaatta atgacattaa gcagatcagg tcgcatccga tagcattgtc
    12181 tcaatgcatg accttcctga acaattaccc gaatataaca ctcgttgagg caagtgatac
    12241 tgcaggaagt gcaaaactaa tctcggaaca caagcttaaa aagatagcag ccattgcttc
    12301 ttcaaaggca gccgagatgt acggtcttga gataattgct cccggcattg aaacctataa
    12361 aaagaattat acacgattcc tggttatcgg taatgagcaa atggggaaaa aggacggcaa
    12421 caagatgtca gtctgctttg ccacaggcca taagcccggg tcacttgcaa cagtactcgt
    12481 caggctcgca caaatgaaca tcaacctttc aaaaatacag tcggtaccac ggctcaacgg
    12541 aggatgggaa tacatgttct atctcgatct ggagcttcct tcgggaatct cgcttgatac
    12601 tattgaggaa attctttaca aggataccag caatctggaa atactgggta tttacaataa
    12661 aggagaaatg atccatgaaa gttgaacctg cagacaggac cttactggtc gaggaatatt
    12721 acttctcccg caaactgaag cagattgagg aaatgcgcaa gggaggggcc gatgttataa
    12781 atcttggtat cggcagcccc gatcagcctc cgtcagccgg cacgattgag cgccttactg
    12841 ctgaggctgc aaaacccaat gttcatggtt atcagagtta cactggtatc cctgcactca
    12901 gaaaagcatt tgccgactgg tacatgaggt acttcgggat aaggcttgat ccggaaggtg
    12961 agatcctgcc gctcattggc agcaaggaag gcattatgca tatcaccatg gcctttgtaa
    13021 accccggtga tgaagttctg gtgcctgatc ccggatatcc aacctactca tcggcaacga
    13081 gtctcgcggg cggcagggtc aggtactatg atctcacaga ggaaaacagg tggcttcctg
    13141 atcttgatct gcttgaaaaa agcgatctgt ccaggaccaa aatcatgtgg ataaactatc
    13201 ctcacatgcc aaccggagca aaagcctcag ctggaaaatt atcggagatg gtttcatttg
    13261 cttccagcca tggtatcctg ttatgcaacg acaatccata cagtttcatc ctgaacaccg
    13321 aatacatgag cgtcctgagt gccgaaaatg caatggacac ggcaatcgag ctaaactccc
    13381 tgagtaaatc ccataatatg gccggatgga ggattggaat ggcagcaggg aacagggagt
    13441 atataaacac tattctgaaa gtgaaaagca acatggactc agggatgttc atgccaatgc
    13501 aaatggctgc cgttgctgcg ctcagtaatc cggattcatg gtatgaaggc ctgaacagtc
    13561 tgtatctcag tcgccggaaa atagcagaat caatccttga aatcctgggt tgcactttcg
    13621 acactgacca ggcagggttg ttcctctggg gcagaatacc ggacgacgct gtaagcgccg
    13681 aagatctttc agaaaaaata ctgacagaag cgcatgtttt tattactccg ggatttattt
    13741 tcggaaagaa cggacggaga tatatccgta tttcactatg tgccggcgag gaccggcttt
    13801 cagaagcccg ggacaggctg acatctgcaa aacacggtca ttcgcattca actttactct
    13861 catgacaata gctgttgtag gaatagggct catcggagga tcattgatga tcgatcttcg
    13921 caagcgaggg tttgcccaga aaataattgg cgtggattta aatatgcagc accggaacat
    13981 agcccagctc tgcggcattg tggatgaaac cgattcgctt gaaaatgccg ttgacaggag
    14041 cgacctggtc attttaagca gcccggtcga tgccaattgc atgatgctcc cgtcaatcct
    14101 cgacagaatt cagggtaccg gcaaggtggt tagcgacatg ggatcgacca aggcaagtat
    14161 tgccagggta tcggaacatc acccgggcag ggggcgattc gttgccctgc acccaatggc
    14221 cggaacagag ttttccggac cgcttgcggc cattggcaag ctgtttgatt acaagatcgc
    14281 aataatctgc gatgccgaaa aaagcgatgt tgatgcaaga aggatagtcg aaagtatgct
    14341 cgacactctt aacatgagga ggatatacat ggattctgct gatcatgaca tacatgtggc
    14401 ctatgtatcg catatttccc acatcacttc tttctccctg gccctgagcg tgctggacaa
    14461 ggagaaggag gaagagcaca ttacaaccct tgcaggcggc ggttttgaaa gcaccgtcag
    14521 gctggcaaag agcagtgccg aaacctgggc tcccattttc ctggaaaact ccgaattcat
    14581 attggaagtc atggataatt atatcgaaaa aatgaatctg ttcaagaaaa tgataaggga
    14641 aaggaataaa gagggcctga tggcattaat ggaagaggca aacaagatca ggaaaatatt
    14701 acactaaaaa acaataacat ggtacttgaa acgggaacaa cacacatcag ggactggaga
    14761 ggctacaagg gcagaccttt cctgatatcg ggcccctgca gcgctgaaac cgaggaacag
    14821 gttatggcaa ctgcaaagga actggcccag ctcaaagaag tcaacctttt cagggcaggc
    14881 atctggaaac cccgaaccag gccgaattca tttgagggaa taggtattga aggactcaaa
    14941 tggctgagaa atgtgaaaca ggaaacaggc ctgatggtcg gtacggaagt tgccaacgaa
    15001 aaacacgttt atgaagcact taaatatggc gttgacatgc tgtggatagg cgccaggact
    15061 tctgtcaatc ctttcaccgt acaggaaatt tcaaatgcac tcaacggtgt ggatgtcatg
    15121 gtactggtaa agaaccccat caaccccgat cttgatctgt ggatcggtgc catcgaacgt
    15181 attgcaaggg cagggataag caggctcggg gcaattcatc gcggattttc aacatacgaa
    15241 aaaacagttt accgtaatca gcccaactgg cagctgccga ttgagctgcg aagacgcatg
    15301 ccggatctgc caatactgtg tgacccaagt cacattgccg gggccaggag atacctgtat
    15361 gaaatcgcac agaaagccct cgacctgaat tttgatggtt taatgataga atctcatatc
    15421 gatcccgata aagctctcag tgattcagca cagcaggtta cccccaatga tctcagggaa
    15481 cttctcagca ggctgatcct gagaagcccg gtaccttccg atccgaaact gctcgatatt
    15541 ctcggagaat taaggcagca gattgatatt ttcgacgact acctcctcga tattctggag
    15601 aagcgaatga aagtcgcaga aacaatcggc cagtacaaaa aggaaaacaa tatcactatc
    15661 ctgcagtcaa cccgctggga tgagatcatc agcaaggtca tgatcaacgg agagtccaag
    15721 ggactcagca gggaactgat agataccgtc tttaaggcca ttcaccagga atcgatcaac
    15781 caccagatga gagtaatgaa taatggtgtc atgaatcttg ccgttgataa acattaattt
    15841 tgcagtgata tggttcgatc tgttgatcca tcctttatcg caggggagat aaaatcacca
    15901 tcttccaaga gcatgacaca gagagccatt gcagcagcaa tgcttgcaaa aggaagcagt
    15961 ataataaaga atccttcgta ttgcaatgac tctctcgctg ctatgagtat tgccaggggt
    16021 tttggagcaa aaatagtgcc ggaagagagt ctgatgtatg tcacaggccg gcccggggtg
    16081 acggacaata ctctcaattg cggagagtca ggtcttgcca tcaggatgtt ctctccgatt
    16141 gcggcattgt ttcccgaaaa gatcgtcatg cttggaaaag gctcactcag aaacaggccg
    16201 atgcaaatga taggggatgc attaatccag ctgggagcca ggtttgaaag taccggcggt
    16261 tttcttccac ttacaattca ggggccgtta aaaggcggac ggtgcgaaat tgacggatcg
    16321 gtgagttccc agcttcttac agggctccta atggccctgc cgcttaccgc tgaagattcc
    16381 gagatcttcg tgaccaacct taaaagcaag ccgtatgttg acatgacaat gaaagttctc
    16441 agggattttg gcataacggt aagccatgaa gactataaac tgttcactat caggggcagg
    16501 caggcctatt ccccggtaaa cattgaagta gagggcgact ggagcggcgg agctttcctg
    16561 ctcgttgcgg gagcaataaa cggcaacgtg gttgtcaggg gccttcaggc aggaagttgc
    16621 cagagcgaca gggcaatcct ggaagccatg gaaaaagccg gagcaatgat cacaataagc
    16681 caggactgga tttcagtaac caaatccgat atgaaagctt tcgagttcga cgccaccgac
    16741 gcgcccgacc tgtttccccc tcttgccgcc ctggcagcct actgcaacgg catttcaagg
    16801 attaagggtg catcgcggct catccacaag gaaagcaaca gggcagcagc ccttataagc
    16861 gaattcggta aaatgaacat tgaaataaga ctcgaggatg acctgatgct gattaccgga
    16921 ggcaggacaa tgggagccgc agtatcatcg cataacgacc accggatcgc aatggcagcc
    16981 gctgttgctg cactaggggc cgaaggcagg gtgtcaatag ctgattcgca atgtgtggca
    17041 aaatcgtatc cggatttctt caatgacctc atgaaagcag gtgtttctgt tattgaataa
    17101 taattgcagg cagctataag atttcccggt cagatatatt ttttctgaaa atcattaaaa
    17161 aaaagaactt ttttattgga atttaaaaaa attaaataat tttggagcca tgaaccatgg
    17221 aatgttatat atctggtctt ggcgcagcat caatcagcaa gctgtgaata aggcctgtat
    17281 ataatcttcg caaagatatt ttcccgccaa aggcatgctg ttcacagcat gcctttttta
    17341 tttaaaactc attaaccaaa ataaataaag tcatgaaaaa aagtacaaaa atttcgctca
    17401 gtgaaaaaga aatcccccgg caatggtata atattgtggc agatatgccc aataaaccgc
    17461 tgccgcctct tcatccggtg acacgcgagc ccattggacc cgatgcactt gccgcggttt
    17521 ttccaatgga actgatcaaa caggaggttt ctgcagaacg gtatattgat atacccgacg
    17581 aagtcctgga tctgtacaag gtataccggc caagtccgat gttcagggcg gtcaatctgg
    17641 aaaaggcaat aggaaccagg agcaagatat actacaagta cgagggaggc aactactccg
    17701 gttcccataa gaccaacact gccattgcac aggcctat
//
LOCUS       DAOOYG010000049        17367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3190_length_17367_cov_5.423059, whole genome shotgun sequence.
ACCESSION   DAOOYG010000049 DAOOYG010000000
VERSION     DAOOYG010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3190_length_17367_cov_5.423059"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PK288_06020"
     CDS             complement(<1..680)
                     /locus_tag="PK288_06020"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPM18511.1"
                     /translation="MDSQFSQRVKDILGYSKEEAIRLGNSHISPEHLFLGILRDGEGV
                     AVEVLINQGIDLPVLKGSLEKSIKVENPVPVADHEIIPLLRSTERILKLVYLEAKSLK
                     NNTIDSEHLLLAILKDENALVTRFLAELDVDYQSVRRAVEAGFPSAKADFPREDDDEG
                     QSYGSPGRGSQSAPSSSKSSSDTPVLDNFGIDLTKAAEEGRLDPIVGREREIERLAQI
                     LSRRKKNNP"
     gene            938..3463
                     /gene="gyrA"
                     /locus_tag="PK288_06025"
     CDS             938..3463
                     /gene="gyrA"
                     /locus_tag="PK288_06025"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPM18512.1"
                     /translation="MSERERIIQVNIEEEMKSAYIDYSMSVIVSRALPDVRDGLKPVH
                     RRVLYGMSELGVLSNRAYKKSARIVGEVLGKYHPHGDSSVYEAMVRMAQEWTMRYPLV
                     DGQGNFGSIDGDSPAAMRYTEARLKRIAEETLSDIEKETVDFQPNFDDSLKEPVVLPT
                     RIPNLLVNGASGIAVGMATNMPPHNLGEIIDATVAFIDNRNITTEELLTHIKGPDFPT
                     GGIIYGQQGIRDACETGRGRIVIRAKTEIELTHSGRECIVVTEIPYMVNKAEMIRKIA
                     DLINEKKLEGISYINDESDRNGMRVVIILKKDATANVVLNNLFKYTSLQTSFNVNNIA
                     LVRGRPRTLSTRDLIDYFFRHRHDVVIRRSRFDLDQAEKRAHILEGLIIASDNIDEVI
                     AIIKASGNPDEARERLMARFGLSEAQSRAIVEMRLRQLTGLEQEKLRAEYTEVMALID
                     YLRNVLADENLQMKIIKDELLEIKEKYGDKRRTEIIPNAEEFNPEDFYSDDEMVITIS
                     HLGYIKRTPLTEFRRQNRGGTGAKGGTTRDEDFIEHLYIATMHNTMLFFTSKGKCYWL
                     KVYNIPEGSRVSKGRAMQNLINIEQDDKVRAFINVKNLSDEDYINNNFIVLCTTKGII
                     KKTSLEAYSRPRTSGVNAITVREGDELLEARMTNGHHHIMLAVKSGRAIRFPEDSVRP
                     MGRTASGVRGIKLDNEVTDYVIGMITVENKEKEILVVSEKGYGKRSDIEDYRITNRGG
                     KGVKTINITEKTGYLIALKDVTDNHDLMIITQFGNILRSPVSALRIMGRATQGVRLIN
                     LRENDIIASVASVFVSGDESGDEADMEQQDDNNEKDFGKEFDD"
     gene            3522..4685
                     /locus_tag="PK288_06030"
     CDS             3522..4685
                     /locus_tag="PK288_06030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM18513.1"
                     /translation="MKKYLLLIAVLAVSLGTYAQKGKVTSALSMIEQGNLDKAKEALD
                     QAMVDEKSKDWFNTYFAKGKLCQAVFKTDNQSFKKLCGADPLGEAYAAYEKAMELDPK
                     GTVKKRIIANMIYNSLALDLYNQGGDQFEAKDFEGALKSFETQIKITESDEFAGAVDT
                     GMYYNAGLAAINSHKYEKAIDYFNKCAEMQYMGITPYYQMYESYLGLGDTLKAEATLK
                     SLPEKFPDDKTITLQLIDLYIKSGKNDEALKYLKVAKEADPTNYSLYFAEGIIYLNSN
                     QYDQAIENLQKSIELKSDLYDSQYGLGAAYINKASDMFAKANDIMDVNKYNAAIDEAM
                     TVFAKALPYMERANELIPDDVYALRGLRELYYRLRMTDKYDAVKAKLDQIEQQ"
     gene            4764..5606
                     /locus_tag="PK288_06035"
     CDS             4764..5606
                     /locus_tag="PK288_06035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPM18514.1"
                     /translation="MINEIPSIDYFLRMIIRPVFLLLLSCIALPACKPEQEEIPADEG
                     KIIVLMYHRIVEDTPENLYERSLRDLESDIKYLITNNITIIDFNDLGSVLTGNKKSYG
                     NSAIITFDDGDCSWYSLVRPLLERYRIKATFFLWTDMIGKDSFISWEEVENMSHYAIS
                     PGIRPFTFGSHSCSHPFLHQSRDSFSTNEDYNSFLDYELGASRQIIEKHTPGSVQVFA
                     LPYGDGAGDSDIIDAAIRNGYEFIRTSIWGTIDTPSTDPFLIPCLPMLDSTDPELIGA
                     YLGL"
     gene            complement(5866..7500)
                     /locus_tag="PK288_06040"
     CDS             complement(5866..7500)
                     /locus_tag="PK288_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPM18515.1"
                     /translation="MNDRFSVSPGGEEFPLPDHKDYRDEFERLSKRIEQERAKNREIV
                     VVMGVGFVGAVMAAIVADTVDKDGRSTKFVIGMQRPSVRSYWKIPMLNRGVSPVKAED
                     PEVDPMIARCVKDKKTLIATFTYDVLKLADVVVVDVQCDYIKEDLGDVRTGQTDMAAL
                     ESSLHVIAEHIPPDALVLIETTVAPGTTEQVAYPIMKKVFRKRGINTDPLLAHSYERV
                     MPGKNYVASIRDFWRVCSGINEEARNRVVKFLTEVLNTEKYPLTVMDRPIESETAKIV
                     ENSYRATILAFLDEWSLFAERNGVDLIKVIKAIKVRPTHSNMIFPGPGIGGYCLPKDG
                     GLGLWAYKHILGFEDDIFRITPLAIDINDTRGLHVGQLARDALRNMGKPIAAANILVL
                     GAAYREDVGDTRYSGSEIVIRKLAEMGAEIKVHDPYVEHWWEFEQQDTFPGVGQSKAR
                     FFRNQEKLKEIRVEHDLEKVLEDVDAVILAVPHKNYLNLDPDYVISKIGKPAAIIDCF
                     GILSDDGIRRYFQLGCEVKGLGRGHIQRIKDEVRKL"
     gene            7693..10389
                     /locus_tag="PK288_06045"
     CDS             7693..10389
                     /locus_tag="PK288_06045"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPM18516.1"
                     /translation="MFIKQILPALVSSLTVITTFMAMIVFSSCLRTNPDPKLYDLRTE
                     NLSSPLGIGTTSPLLSWKIKSSVNGTAQSSYQVLAASSEALLNEEEADLWNSGKVSSS
                     RNFLLSYAGTGLNPGSVCYWKARIWDQNDRPTECSEATCFSIGLLEQSDWQAQYIGLP
                     DEEEYSTSPQLRKTFNLEKVPERALLHVNSLGYHEIWLNGQKAGENVLVPAVSQFSKR
                     SLTVTYDITRLLVVGRNDLVIWLGRGWYSRGLPGVAWKGPLVRAQAQILTDGIWQKFL
                     PTDSTWTGRKSGYSTMGTWRPGDFGGELVDGSMLLEDLSPVGLDKVSWNPVTVASVNE
                     GIASPQPVEGNRIMETIGPESVTRLADSVWLADMGTTLTGWFEMNIPPVPHGHVIKLE
                     YCDHLDKEGNFVNQRQEDRYISAGRGNEVFCNKFNYHGFRYVKISNLPSEPTREKLKA
                     HLIHTGYAVSSSFSCSDPDLNRIHDMISYTLRCLSLGGYLVDCTQIERLGYGGDGNAS
                     TETAQTLFGLEPLYRNWLQAWADCIRDDGGMPHTAPNPYPAGGGPYWCGFIITAAWRT
                     YVNYGNAAILEKYYPVMQKWLEYVGKYSPEGLLEPWPETDYRTWYLGDWATPEGTDQK
                     DPESIGLVNNCFIVVCYDTMQKIAELLGKTTDAGEYSDKAAALRNKINDRYYSVKKAT
                     YGSGSQIDLTYPLLAKVVPDTLKEKITKSLHNLIIINKKGHIATGLVGIPVFTEWAIR
                     NRESELMYTILKSKGYPGYLHMLDNGATTTWEHWNGARSRMHNCYNGIGAWFYQALCG
                     ITPGETDPGYTRLTVDPQIPHGVSRAECTKDTPSGPISIAWVKTPGKLKVEIVIPVGC
                     IADFKIPDNTLRCRINGKQGKASGTETLRSGRYIIRCILNNN"
     gene            10478..11152
                     /locus_tag="PK288_06050"
     CDS             10478..11152
                     /locus_tag="PK288_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPM18517.1"
                     /translation="MEQQIRKSTLDFLRAIRQNNEREWFNLNRAWYEDARDNFSAFIQ
                     AVIDRTTRFEPILRGLEAKSCTYRIYRDTRFSNDKTIYKSHMGALIVRGGKQNGNRFA
                     GYYFHLEPDGASLAGGGAYLPPMPWLSAIRTRIAEDGDQFIRIINNKAFKKCFGEIGG
                     EKLKSPPRGFISDNPFIELIKFKSFLASKTLSDDIVTGKECLDQVVETFRLMKPFNDF
                     LNSVYS"
     gene            11330..12985
                     /locus_tag="PK288_06055"
     CDS             11330..12985
                     /locus_tag="PK288_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HPM18518.1"
                     /translation="MKKESALYATDGKYNPRFIVLITLVATLGGFLFGYDTAVVNGAE
                     KALVDFFIARSTDPSHYDYAVKLITQYRLLVVFSILLVGIVISSLIIRLSDRKKGLPV
                     VLVLAVTMIFLLFRYLNQPVPPEGAVEELQGIADSIKGFIISGALIGCILGSSVAGFV
                     SRWAGRKNGLLLSAVMFTLSGIGAWRPESFNFFGTLDVFSFLIYRIIGGIGIGLASIL
                     SPMYIAETAPAGKRGRLVSWNQFAIISGMVIIYFVNYLIARSGDEKWLTAEGWRWMFF
                     SGVIPSVAFLLLLLLIPETPRYLLLKGREKEAMRIISRISGSGNAGIMVSEIRETLQE
                     KNAPWLSYGFMVIFTGIMLAIFQQFVGINVVMYYAGNIFRNMGASVNTSLIQTIIVGL
                     INMLFTILAISSVDRYGRKPLMIAGALTMALSMLALGFAFYSGNTGFIALLFMLSFTA
                     GFAMSWGPVTWVLLSEIFPNSIRGAMSIPGAALWISNLIVSWTFPVMNDNVYLTSLFN
                     HGFAYWIYGIMGLLAALFVYRFIPETKGKTLEEIEGLWKTPGR"
     gene            13144..14154
                     /locus_tag="PK288_06060"
     CDS             13144..14154
                     /locus_tag="PK288_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPM18519.1"
                     /translation="MTGLQRTIEFIRGTDVDHPPFHPIIMRWAARYAGVKYRDFCTVP
                     AAKCMAMIRCARDFGIDWVTVMSDPWAEASAFGVEIHYPEDSLPVDTGGHLHDAAHAA
                     ALNPYDPLKSSRCTNRISEIHEFSKSVGDELFIVGWVEGPVAEYVDLRGASEASMDFL
                     TEPALVGKAMDVIIESAMEFISLQISAGAHCIGIGDSFCSQIGPELYYSFAFERHKKL
                     IDHIHNKGALAKLHICGNTEPILPGMIRTGVDIIDIDHLVPSISDFAAMIGPGQVFSG
                     KSDPVTVIQAGPADTINRSVAADFIDSGRRCIVSAGCEITPETPVENMKYFHAAARAI
                     RL"
     gene            14263..14799
                     /locus_tag="PK288_06065"
     CDS             14263..14799
                     /locus_tag="PK288_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HPM18520.1"
                     /translation="MKKLIITLFSLMLMIPLLTAQTGKLQVGMKAPEWVFTDADKKEF
                     TMDSWQGKVLQVNYVDPDESDLNDLFNDAVNKAADVDKIIDRDKFKGFGIVDCKSTWK
                     PNGLIRTIAGNKARKYNTTILFDYKATLQDLWGLPKDNYSVVILDKNRICRAIYTGRI
                     PESEHQKIISMIIDLTKE"
     gene            14796..15335
                     /locus_tag="PK288_06070"
     CDS             14796..15335
                     /locus_tag="PK288_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18521.1"
                     /translation="MIAPNLVIIAGTGSKSGKTSAACRIIEQFRDAGIAAVKITPHQH
                     ETTRGLTEVDRKPGYDIFLETNPETDKDTSRMLRAGASRVYFVRATDEYLSEAFGMIL
                     ESVPAATPLVCESAALRYFIEPGLFVIMTSVVSHRTKDIRSLQRLPHVEFELESLSAG
                     MELPLSFHNGRWICWHYGR"
     gene            complement(15336..16163)
                     /locus_tag="PK288_06075"
     CDS             complement(15336..16163)
                     /locus_tag="PK288_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HPM18522.1"
                     /translation="MYFTSNIKFLRKRRGRTQDEVAAALNLKRSTLSGYENGVAQPGI
                     EILVSFSGYFSMSVDTLLKVDMTKLSESQLGELERGYDAYIRGSNLRVLVTTVDKENR
                     ENIELVAEKAKAGYTTGYADPEYIGELPVFRLPFLSDRRKYRTFQLKGDSMLPIPDGS
                     WVTGEFLQDWNEIKSGNAYIVFTINDGIVFKIIENRLMTDGRLVLYSLNPVYEPYEVS
                     INEVKEIWKFVNYISEELPEPVLPEKQLIRSIASMKDDLEKIKARLGRDISDACEVI"
     gene            16379..>17367
                     /gene="dinB"
                     /locus_tag="PK288_06080"
     CDS             16379..>17367
                     /gene="dinB"
                     /locus_tag="PK288_06080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPM18523.1"
                     /translation="MERSVLHLDLDTFFVSVERLKNSRLIGKPVIIGGLSDRSVVSSC
                     SYEARKYGVNSAMPMKMARSLCPEAIMIRGDMEMYSRYSNMVTEIIAEKSPLYEKSSI
                     DEHYVDLTGMDRFFGCCKWAHELRQRIMKETGLPVSAGLSVNKTVSKIAAGEAKPNGE
                     IEVGREIVFPFLDPLPVSRIPMVGPKTCHILRSMGIATIYTLRNIPAEMVEKVLGKNG
                     IEIWKKANGIDPSPVMSFSEQKSISTEHTFEKDTTDIARLNQLLAGMVEKIAYDLRNQ
                     EKLTSCVTVKIRYSNFDTHTLQKRIPYTSFDHVLTDVAKELLSRLYRRRMLIRL"
BASE COUNT         4892 a         4042 c         4093 g         4340 t
ORIGIN      
        1 ggattgttct tttttcttcg cgacaggatc tgtgcaagtc tttctatttc tcgttccctt
       61 cccacaatgg gatcaagtcg tccttcctcg gctgccttgg taagatcaat gccgaagtta
      121 tcaagaaccg gcgtgtcgga agaggatttt gaagatgaag gggcagattg ggagcctctt
      181 cccgggctgc cgtatgactg gccctcatca tcgtcttccc gcggaaaatc agctttagcc
      241 gaagggaatc ctgcttcgac ggcccttctt accgactggt aatcaacgtc gagctctgcc
      301 aggaacctgg tcacaagggc attttcgtcc ttaaggattg ccagaagaag atgttctgaa
      361 tcaatggtat tgtttttaag ggatttggct tcgagataaa caagcttgag gattctctca
      421 gtgctgcgca acaaagggat tatctcatga tcagccaccg gaacgggatt ttcaaccttg
      481 atgctttttt caagggatcc cttcagaacc ggaagatcaa taccctggtt tataaggacc
      541 tcaaccgcta ctccttcacc atccctgaga attccaagaa acaggtgttc aggacttata
      601 tggctgttac ccagccttat agcctcttcc ttgctgtatc caagaatatc ttttacacgt
      661 tgtgaaaatt gtgaatccat atttatttta aatacttttc aatattatgt ctaaaaacag
      721 tcattatgtc agtataaacg atcaaaaata ccattgatgt ataaatcaaa ggtaaattta
      781 atacaattaa ctacaggcaa ttttcgtgcc caattataaa caacgaatgt tcaaaagtca
      841 tcatgtcacc cgagcaaaat tacccaaatt atcactaatt tcgtcctcga aacactcatg
      901 aagtgtgatt gttcgtgatg atttcataat taatactatg tcagaaagag aaaggataat
      961 tcaggtaaac attgaagagg agatgaagtc ggcctatatt gactattcca tgtcagtaat
     1021 agtgtcgagg gcgttacctg atgtcaggga tggactgaag cctgttcaca gaagggttct
     1081 ttacggtatg tccgagcttg gagttctctc aaacagagcc tataagaagt cggcgaggat
     1141 agtaggggag gtgcttggga aatatcatcc ccacggagat tcgtcagttt acgaggcaat
     1201 ggtcagaatg gcacaggaat ggacgatgag atatccgctg gttgacggtc agggcaattt
     1261 cggttcgatt gacggagaca gcccggcagc aatgagatat accgaagcca ggctgaaaag
     1321 gatcgcggag gaaaccctgt cggacattga aaaggaaact gttgactttc agccaaattt
     1381 cgatgattca ttaaaagagc cagtagtact gccgacaaga atccccaacc tcctggtaaa
     1441 cggtgcatcg ggtattgccg tgggcatggc tacaaatatg ccgcctcaca acctgggaga
     1501 gatcatagat gcaaccgtag cctttattga taaccgcaac ataactaccg aagaacttct
     1561 cacccatatc aaaggccccg attttccgac aggcggaata atttacggcc agcaggggat
     1621 aagggatgcc tgtgaaaccg gcagaggaag gatagtcatc agggcaaaaa ccgagataga
     1681 gctgacccat tcgggccgtg aatgcattgt tgtcactgaa atcccataca tggtcaataa
     1741 ggcagaaatg atcagaaaga ttgccgacct gataaatgag aaaaaactgg aggggatttc
     1801 ctacatcaat gacgaatcgg accgcaacgg aatgagggtg gtgataatcc tgaaaaagga
     1861 tgccactgcc aatgttgtgc tcaataacct tttcaaatac acctcgcttc aaacttcctt
     1921 caacgtaaat aatattgccc ttgtcagggg gaggccgcga accctcagca ccagggatct
     1981 gatagactat ttcttcaggc accgccatga tgtagtgatc agaaggtcgc ggtttgacct
     2041 tgatcaggca gaaaagcggg ctcatatact ggaaggactc attatcgcca gcgacaatat
     2101 tgatgaggtg attgcaataa tcaaggcatc ggggaatcct gatgaagcaa gagagagact
     2161 tatggcccgg ttcgggcttt ccgaagccca gtcaagggcc atcgtggaaa tgcgcctgcg
     2221 ccagcttacg ggactggagc aggaaaagct gagggctgaa tacaccgagg ttatggcatt
     2281 gatagattat ctgagaaatg tccttgctga cgaaaacctc cagatgaaga tcatcaagga
     2341 cgaacttctg gaaattaagg aaaagtacgg tgataaaaga cgtaccgaaa taatacccaa
     2401 tgccgaggaa ttcaatcccg aagatttcta ctccgatgat gaaatggtaa taaccatttc
     2461 ccatctggga tatataaaac gcaccccgtt aacagaattc aggcgtcaga acaggggcgg
     2521 aacaggggca aagggaggca caacaaggga tgaggatttc attgagcatc tttatattgc
     2581 cacaatgcac aatacgatgc tcttttttac aagcaagggt aaatgttact ggctgaaggt
     2641 ctataatatc cccgaaggct cgagggtctc aaagggaagg gcaatgcaga atctcataaa
     2701 catcgaacag gatgacaagg taagggcatt catcaatgta aagaacctca gtgatgagga
     2761 ctatatcaac aacaatttca tcgtgctctg caccactaag ggaatcatta agaaaacttc
     2821 actggaagcc tattccagac ccagaaccag cggtgtcaac gccatcacgg tcagggaagg
     2881 tgatgaactt cttgaagcaa ggatgacaaa cggacatcat catatcatgc tggctgtcaa
     2941 atcgggaaga gcaataagat ttcccgagga ctctgtgagg cccatgggca ggaccgcatc
     3001 aggagtcagg ggtataaaac tcgacaatga ggtgaccgat tacgtaatcg gaatgattac
     3061 agtcgaaaac aaggaaaaag aaatacttgt cgtctctgaa aaaggctacg gaaaaagatc
     3121 ggatatcgaa gattaccgca ttaccaaccg tggcggcaag ggagtgaaaa cgatcaacat
     3181 cactgaaaaa acagggtacc tgattgcatt gaaggatgtg acagacaacc atgacctgat
     3241 gattataacc cagttcggca acatcctgag aagccccgtt tcggcactcc gcataatggg
     3301 cagggcaacc cagggagtca gacttattaa cctcagggag aatgacatca ttgcctcggt
     3361 ggcaagcgtg tttgtaagcg gggatgaatc aggtgatgaa gcggatatgg aacaacagga
     3421 tgataataat gaaaaggatt ttggcaaaga atttgatgat taacaagtaa agttttacat
     3481 ttgcaaaact attattgaca cattaacaag atttttaaac tatgaaaaag tatttactgc
     3541 ttatcgcggt tttagctgta tctcttggca cctacgcaca aaaaggaaag gtgaccagtg
     3601 ctctttcgat gatcgaacag ggaaatctgg ataaagcgaa agaggcgctt gatcaggcta
     3661 tggttgatga gaaatcaaaa gactggttca atacctattt tgccaaaggg aaactctgtc
     3721 aggcagtttt taaaacagat aatcaaagtt tcaagaaact ctgcggagcc gatcctctcg
     3781 gcgaagctta cgctgcctac gaaaaggcaa tggagctgga tcccaaaggc actgtaaaaa
     3841 agaggatcat cgcaaatatg atctataatt ccctggctct cgatctgtac aaccagggag
     3901 gagatcagtt tgaagccaag gactttgaag gcgcactcaa gtcgttcgaa acccagatta
     3961 aaattactga aagcgatgag ttcgccggag ccgtcgatac cggaatgtat tacaatgcag
     4021 gtctggctgc cataaactcg cacaaatacg aaaaggcaat cgattatttc aacaaatgtg
     4081 ctgaaatgca gtatatggga attaccccat actatcagat gtatgaaagc tatcttggac
     4141 tgggcgatac cctcaaagcc gaggctacac tcaaaagcct tcctgagaaa ttccccgatg
     4201 ataaaaccat taccctgcag ctcattgacc tttatatcaa aagcggaaag aatgacgagg
     4261 cactcaaata cctgaaagtg gcaaaggagg ccgatcccac caactacagc ctgtattttg
     4321 ccgaaggcat aatttacctc aattcaaacc agtatgacca ggcaattgaa aacctgcaga
     4381 aatccatcga gctgaaatcc gatctttacg acagccagta cggacttggt gcggcatata
     4441 tcaacaaagc ctcggatatg tttgcaaagg caaatgacat aatggatgtc aataagtaca
     4501 atgcggccat agatgaagcc atgactgtgt ttgccaaagc attgccgtac atggagaggg
     4561 ccaatgaact cattcctgat gatgtctatg cactgagagg cctcagagag ctttattacc
     4621 ggctcaggat gactgacaaa tacgacgcag ttaaagctaa actcgaccag atcgaacaac
     4681 agtaaataat aattccaatt catataaaga ggttgtatca gaagataact acttttgaaa
     4741 caacctcttt tcttttacgg gaaatgataa atgaaattcc atccatcgat tatttcctca
     4801 ggatgatcat ccgcccggta ttcctgctgc tattgtcttg tattgcatta ccggcctgta
     4861 agcctgaaca ggaagaaata cccgcggatg aggggaaaat cattgttctt atgtatcacc
     4921 ggattgtaga agacacgccg gaaaacctgt acgaaagaag cctcagggat cttgagtctg
     4981 atattaaata tctgataacc aataatataa ctatcattga cttcaatgat ctgggttcag
     5041 ttctcaccgg taacaagaag agttacggaa attccgcaat cattaccttc gacgacgggg
     5101 actgctcatg gtattcactt gtcagaccct tgctggagag atacaggatc aaggccacct
     5161 ttttcctgtg gaccgacatg atcgggaaag attccttcat ctcatgggaa gaagttgaaa
     5221 atatgagtca ctatgcaata agcccgggaa taaggccatt tacattcggg tctcactcct
     5281 gttctcatcc tttcctgcat cagagcagag attcattctc cacgaacgaa gattacaatt
     5341 catttctcga ttatgagctt ggtgcctcca ggcagatcat agaaaaacat actcccggaa
     5401 gtgtgcaggt tttcgccctg ccgtatggcg acggggcagg agactcagac attatcgatg
     5461 ctgccatacg caatggttat gaatttatca gaacatccat ctggggcacg attgacactc
     5521 cttcaactga tccttttctg attccatgcc ttccgatgct tgactcaacc gatccggaac
     5581 tgataggagc ctatctcgga ctttaattta acagttgcag tcggaagagg atggaagcat
     5641 taaaagagac acctgggcat gtctcaaagc taaaattcgt atattatatt ttcaatttga
     5701 cataatatta attataggaa tattatttga ctttttaaac tgagcccgct caccccaaaa
     5761 aggatttatt ccaaaaaaaa agcagatatg cactgtaggt ccatatctgc ctgcgttggt
     5821 cgtattatct gggtgcaata ttaaccccgc gggatttcga gagcattata acttacgaac
     5881 ttcatccttg atcctctgga tatgtccgcg tccaagtcct tttacttcac aacccagctg
     5941 gaaatatctc cttatcccgt catcggaaag tattccgaaa caatcaataa tagcggctgg
     6001 tttgcctatc ttgcttatga cataatcagg atcaagattc agatagtttt tatgcggtac
     6061 tgcaagtatc acggcatcaa catcttcaag aaccttttcc aggtcatgct ccacacggat
     6121 ctcctttagt ttttcctggt ttctgaaaaa tcttgccttg ctttgtccga ctcccggaaa
     6181 agtatcctgc tgctcaaact cccaccaatg ctcaacatac ggatcatgaa ccttaatttc
     6241 tgcgcccatt tccgcaagct ttcttattac gatttctgag ccgctgtagc gggtatcgcc
     6301 cacatcctcc ctgtatgcgg ctccaagcac caggatattg gcagcggcaa tgggcttccc
     6361 catattccgc aacgcatccc tggcaagctg acccacatga aggcccctgg tgtcattaat
     6421 atcaattgcg agtggtgtga tcctgaatat atcatcctca aaaccaagta tatgcttgta
     6481 tgcccataat cccagtccgc cgtccttcgg aagacagtaa cctccaattc ccggtcccgg
     6541 gaaaatcata ttcgaatgtg ttggccgcac cttaattgcc ttgataacct ttatcaggtc
     6601 aactccgttg cgttcggcaa aaaggctcca ttcatcaagg aatgcaagaa tagtggccct
     6661 gtaactgttt tccactatct tggcggtttc tgattcgatc ggacggtcca tgaccgtaag
     6721 aggatatttc tctgtgttaa gcacttccgt caggaacttc accacccggt tcctggcttc
     6781 ctcattaata ccgctgcata ccctccagaa atcacggata cttgccacat aattctttcc
     6841 cggcataact ctctcatagc tatgtgcaag cagaggatcg gtatttattc cccttttcct
     6901 gaataccttt ttcattatgg gatatgcaac ctgctccgtc gtaccaggtg caacggttgt
     6961 ctcgatcaga accagcgcat ctggaggtat atgttcggct ataacatgaa gcgaagattc
     7021 aagggcagcc atatcagtct gacctgtccg gacatctccg agatcttcct taatatagtc
     7081 acactgcacg tcaaccacca caacatcagc cagcttaaga acatcatacg taaaagtggc
     7141 tataagcgtc ttcttgtcct tgacgcatcg tgctatcatg ggatccactt cagggtcctc
     7201 tgcctttacc ggcgacactc cccggttcag catcggtatt ttccagtaac tccggacact
     7261 gggccgttgc atgccaatga caaattttgt ggaccgcccg tccttgtcaa cagtgtcggc
     7321 aactattgca gccatgactg ccccgacaaa tccaaccccc attacaacta caatttccct
     7381 gttcttggct ctttcttgct ctatgcgctt gctaagcctc tcaaactcat cacggtaatc
     7441 cttatgatca ggcaaaggaa attcctcgcc gcctggactt acagaaaatc tatcgttcat
     7501 gtttatctcc gttaacttat atagtataga ttacttttaa aaaaatgcaa tttagcttaa
     7561 atagtcttga tttttaaact caaaaatctg atttttagct tgccagtcca tttatacttt
     7621 cattaggggc attttcatta tattgatcca gcaatttact catttaccat ctttacaata
     7681 aactacattc ccatgttcat caaacagatc cttccggcac ttgtttcatc actaactgtc
     7741 ataactactt tcatggccat gattgtcttc agcagttgtc tgaggacaaa ccctgatccg
     7801 aagctttatg acctgcggac cgagaacctc tcctctcccc tgggcattgg aacaacctct
     7861 cccctattga gctggaaaat taaatcttcg gtgaacggaa cggcacagtc atcataccag
     7921 gtgcttgccg caagcagtga ggctcttctc aatgaggagg aagccgatct ctggaattca
     7981 ggcaaggtgt catcatcgcg gaattttttg ctgtcatatg ccggtacggg gcttaatccg
     8041 ggctcagtat gctactggaa agctaggata tgggatcaga atgacaggcc tacggaatgt
     8101 agtgaggcta cgtgtttcag catcgggctt cttgaacagt cagactggca ggcacaatat
     8161 ataggacttc ccgatgagga ggaatattct accagccccc agctgaggaa gacctttaac
     8221 ctggaaaagg ttccggaaag ggctttactt cacgtaaact cactgggata ccatgagata
     8281 tggctgaatg gacagaaagc aggcgaaaat gtacttgtac cggcggtgtc acagttcagc
     8341 aaaaggtcgc tgactgttac atacgatatt acccgtttgc tggttgtcgg caggaacgac
     8401 ctggttatct ggctgggccg ggggtggtac agccggggtc tgcccggcgt tgcctggaaa
     8461 ggtccgcttg tcagggcaca ggcccagatc ctcactgacg ggatatggca aaaattcctg
     8521 ccgaccgact caacctggac cgggagaaaa agcggctatt cgaccatggg cacatggaga
     8581 cccggcgatt tcggtggtga acttgttgac ggatccatgc tgctggaaga tctttcaccg
     8641 gtcggtcttg acaaggtcag ctggaatccc gtcacggtgg catccgtgaa tgaaggcatc
     8701 gcttctcccc agccggttga aggcaacagg ataatggaga ctatcgggcc cgaatctgtc
     8761 accaggttgg cggattcggt gtggcttgcc gatatgggca caacgctgac cggctggttt
     8821 gaaatgaaca tccctcctgt tccccacggt catgtgatta aactggaata ctgcgaccac
     8881 ctcgacaagg aggggaactt tgtgaaccag agacaggaag acaggtatat ttcagccggc
     8941 cgcggcaatg aagttttctg caataaattc aactatcacg gattcaggta tgtaaaaata
     9001 tccaacctgc cctccgagcc gacaagggaa aaattaaagg cccacctgat tcataccggt
     9061 tacgccgtat cttcatcgtt cagctgttcc gatcctgatc tgaacagaat tcatgatatg
     9121 atatcctaca ccctcagatg cctcagcctt gggggttacc tggtcgattg cacgcagata
     9181 gaacgcctgg gatacggggg cgatggaaat gcttcgacgg aaactgccca gaccttattc
     9241 ggcctggagc cgttataccg caactggctg caggcatggg ccgattgcat cagggatgac
     9301 ggcggaatgc cccacaccgc acccaatccc tatccggccg gcggcggccc ttactggtgt
     9361 ggttttataa tcacggctgc atggaggaca tacgtaaact atggaaacgc cgcgatcctg
     9421 gaaaaatatt atcccgtgat gcagaaatgg ctggaatatg ttggcaaata ttccccggaa
     9481 ggccttctgg aaccctggcc cgaaaccgat tacaggacct ggtatcttgg cgactgggcc
     9541 accccggaag gaaccgatca gaaggacccg gaatcgatcg gccttgtaaa caactgtttt
     9601 atcgttgtct gctatgacac aatgcagaaa attgcggagc tgctgggcaa aaccaccgac
     9661 gccggggagt attctgacaa agctgcagcc ttaagaaaca agatcaatga caggtattat
     9721 tccgtgaaaa aggccacata cggcagcgga tcgcagattg accttactta tccgcttctg
     9781 gctaaggtag tacctgacac actgaaggaa aaaataacta aaagtctcca taaccttatc
     9841 ataatcaaca agaaaggaca tattgccacc ggcctggtcg ggatcccggt tttcacggaa
     9901 tgggcaataa ggaacaggga atccgaactg atgtacacaa tacttaaaag caaaggctat
     9961 ccgggttatc ttcatatgct cgacaacggg gccacaacca catgggagca ctggaacgga
    10021 gcacgaagca ggatgcacaa ctgttataac ggaataggag catggtttta ccaggccctt
    10081 tgcgggatta ctcccggtga gacggatccc ggctacacca ggctaactgt tgatcctcag
    10141 atacctcacg gagtcagccg ggctgaatgc accaaggata ctccttccgg cccgataagc
    10201 attgcctggg taaaaacccc cggaaaatta aaagttgaga tagttatccc cgtgggctgc
    10261 attgccgact ttaaaatccc cgataatacc ctgagatgca ggatcaacgg gaaacaggga
    10321 aaagcttccg gcacagaaac cctcagaagc ggcagataca ttatccggtg catacttaac
    10381 aacaattagc cgtacagaat cttaaaaaca aaacacaggc caatatcttt ccgattttta
    10441 attatctttt ctgccggttt taaatccgat ttcagtcatg gaacagcaga tcaggaaatc
    10501 cacccttgat ttcctccgcg ccatcaggca aaacaatgag agggaatggt tcaacctgaa
    10561 ccgggcctgg tatgaggatg cccgcgacaa tttcagcgct tttatacagg cagttattga
    10621 caggacaaca agatttgaac ccattctcag gggcctggag gcaaaaagct gcacctacag
    10681 aatatacagg gacaccaggt tctcaaacga caaaaccatc tacaaatccc acatgggagc
    10741 cctcatagtg aggggaggaa aacaaaacgg aaacagattt gccgggtatt attttcacct
    10801 cgaacccgac ggagcgagcc tggccggtgg cggagcatac ctgcctccca tgccctggct
    10861 gtcggcaata agaacaagaa tagcagaaga cggggatcag tttataagaa taatcaataa
    10921 caaagcattt aagaagtgct tcggtgagat cgggggagaa aagctcaaat cacctcccag
    10981 gggcttcatt tccgataatc cattcatcga attgatcaag ttcaagagtt tcctggcaag
    11041 taaaacatta tccgacgata tagtcaccgg caaggaatgc cttgaccagg ttgttgaaac
    11101 cttcaggctg atgaaaccat tcaatgactt tcttaattca gtatatagtt aaagttatga
    11161 tcaagataat cctgttaata tattgtttaa ccatttatta tccgacatga tcggtttata
    11221 tcattaatat ttgcaattgt gtgtattgat atatatcaat acatacagtg atatatatca
    11281 atataattaa ataaaccttt ccctgccgca aaacaaaaag aactcagtct tgaaaaaaga
    11341 atcagccctt tatgccacgg acggaaaata caatccccgt tttatcgttc tgatcacact
    11401 ggttgccaca ctgggcggtt ttctgttcgg ttatgatacg gcagtcgtca acggtgctga
    11461 aaaggccctt gttgattttt tcattgcaag gtcaacagac ccgtcccact acgattatgc
    11521 agtaaaactc ataacccagt accggctcct ggtagtattt tcaatactgc tggtcggaat
    11581 tgtaatttca tctttaatca tcagactatc tgatcgtaaa aagggattac ctgtagtgtt
    11641 ggttcttgcg gtgacaatga ttttcctcct gttccggtat ttaaaccagc cggtccctcc
    11701 tgagggagct gttgaagaac ttcaggggat tgcagattca atcaagggtt ttatcatttc
    11761 gggggccctt atcggctgca ttctcggcag ttctgttgcc gggtttgttt ccaggtgggc
    11821 gggccggaaa aacgggctgc tcctttcggc agtcatgttc acactttccg gcattggtgc
    11881 atggcgacca gaatcattta atttttttgg cacccttgac gttttttcat ttctgattta
    11941 caggatcata ggaggcatcg gaatcgggct tgcatcaatc ctttcgccca tgtatatagc
    12001 tgaaactgcg cctgcgggga aaaggggacg actggtatcg tggaaccagt ttgcaattat
    12061 ttcggggatg gtgatcattt atttcgtcaa ttacctgata gcaaggtcgg gtgacgaaaa
    12121 atggctcacc gccgagggct ggaggtggat gttcttttcg ggggtcatac cttcagtagc
    12181 ttttctgctg ttgcttctgc tgattcctga gacgccgcgc tacctgctgc tgaaaggcag
    12241 ggaaaaggag gccatgagga ttatatccag gatatcgggc tctggcaatg ccggcatcat
    12301 ggtttcggag atcagggaaa ccctgcagga aaagaatgct ccctggcttt cctatggctt
    12361 catggtgatt ttcacaggga taatgcttgc catttttcag cagtttgtcg ggatcaatgt
    12421 tgtgatgtac tatgcaggca atatcttcag gaacatgggt gcaagtgtca atacttctct
    12481 tatccagacg ataattgtgg gcctgataaa catgcttttc actatcctgg caatatctag
    12541 cgttgacagg tacggcagga aaccgctgat gatcgccggt gcgctaacaa tggccctgag
    12601 catgctggca ctcggatttg cattttattc gggaaatacg ggatttattg cgctcctttt
    12661 catgctttcg ttcactgcag gttttgcaat gagctggggg cccgtaacct gggttctgct
    12721 ttcagagatt ttccctaatt ccatccgggg cgcaatgtcc attccgggag cagccttgtg
    12781 gatatcaaat ctcatagtgt catggacttt tccggtaatg aacgataatg tttatctgac
    12841 ctcattgttc aaccatggat ttgcatactg gatatacggg atcatgggcc tgctggcagc
    12901 actgttcgtc tacaggttca ttcccgaaac aaaggggaaa acactggagg agattgaagg
    12961 attatggaaa acgcccggcc ggtagacggc gccttcagtc ctgctgatct tcagatctgc
    13021 aggttcagag gcctgctgcg gaaatgtatc agtcaatgaa gcggttccgg gcaccaggcg
    13081 gcgtatcgga ccggaggatt gaaaatgaat caggggctgt aaaatactga tataataaaa
    13141 aacatgactg gactacagag aacaatcgaa ttcatcagag gaaccgatgt cgatcatcca
    13201 ccctttcatc cgataataat gcgatgggca gcgaggtatg caggggttaa gtacagggat
    13261 ttctgtactg ttcccgccgc gaaatgcatg gcaatgatcc ggtgcgccag ggatttcggg
    13321 atcgactggg tgacagtgat gtcggatcca tgggcagagg catcggcatt cggggtcgag
    13381 atacattatc cggaagattc ccttcctgtt gataccgggg gacatctgca tgacgccgca
    13441 cacgcagcgg ccctgaatcc atatgatccg cttaagagca gcaggtgcac aaacaggatc
    13501 agtgagatcc atgagttcag caaaagtgtc ggggatgaac tcttcattgt tggctgggtc
    13561 gagggtccgg ttgcagaata tgtcgatctg cggggagcct ccgaggcatc aatggatttc
    13621 cttactgaac cagccctggt cggaaaggca atggatgtga tcatagaatc cgcgatggaa
    13681 ttcatatccc tccagataag cgcaggcgca cactgcatag ggatcggcga ttccttctgc
    13741 tcacagattg gccctgagct ttattacagt tttgccttcg agcgtcataa gaaactgatc
    13801 gatcatatcc acaacaaagg cgcgctggcc aaacttcata tctgcggaaa cactgaaccg
    13861 atattgcccg gtatgatccg cacgggtgtt gatattattg atatcgatca ccttgtacca
    13921 tcaatttcgg attttgccgc gatgatcggg cccggacagg tattttccgg caagagcgat
    13981 cctgtgacag tgatccaggc aggaccggcc gatacgatca accgctcggt ggctgcggat
    14041 ttcatcgact ccggcaggcg gtgcatagtg tcggcaggat gtgagatcac gccggaaacg
    14101 cccgttgaaa atatgaaata ttttcatgct gccgcccggg ccataaggtt gtgaaaatcc
    14161 cgggcagaca aaacctggtt tacggtcggg ggtgaattat tccataaaga aggttttttt
    14221 gaaaaaattc atatattaca ccaaatttaa aaaaacagaa caatgaagaa actgattatt
    14281 acactgtttt cccttatgct gatgataccc ctcctgactg cccagacagg aaagctccag
    14341 gttggaatga aagcgcccga atgggttttt acggatgcgg acaaaaagga attcaccatg
    14401 gactcctggc agggcaaggt attgcaggtc aattatgtcg atccagatga atcagatctg
    14461 aacgatcttt tcaatgatgc cgtaaacaaa gccgcggatg tggacaagat aatcgacagg
    14521 gataagttca agggcttcgg gattgtggat tgcaaatcga cctggaagcc aaacggcctg
    14581 ataaggacga ttgcaggcaa caaggccagg aaatacaata ccaccatcct gttcgactat
    14641 aaggcaacac tccaggatct ctggggcctg ccgaaagaca attacagtgt cgtaatactc
    14701 gacaagaacc ggatctgccg ggcaatctac accggcagga tccctgaatc cgagcaccag
    14761 aaaatcatca gcatgataat tgatcttacc aaggaatgat cgctcctaac ctggtcataa
    14821 ttgcaggcac gggcagcaaa tcgggcaaaa cctcggcagc gtgccggatc atcgaacaat
    14881 tcagggatgc aggcattgcg gctgtaaaga tcactcccca tcagcatgaa acaacccggg
    14941 ggctcactga agttgacagg aagcccggtt atgatatttt tctggagacg aatcctgaaa
    15001 cagacaagga cacttcccgg atgctccgtg ccggggcatc aagggtatac tttgtcaggg
    15061 ccaccgatga atatctttcc gaagcattcg gaatgatact tgaatcagtt cctgccgcga
    15121 ctccccttgt atgtgaatca gcagccctga gatacttcat cgaacccgga ttgtttgtaa
    15181 taatgacctc tgtagtaagc cacaggacaa aggatataag atcattacag agactacctc
    15241 atgtagagtt tgagctggag agtttgtcgg caggcatgga attgccgttg agtttccata
    15301 atggcagatg gatatgctgg cactacggtc gctgatcaga tgacttcaca ggcatcggaa
    15361 atatccctgc cgagcctggc cttgattttt tcaagatcgt ccttcattga agcaatggac
    15421 cggatcagct gtttctcggg cagaacgggc tcaggcagct cttcacttat gtaattgaca
    15481 aatttccaga tctccttcac ctcatttata ctgacttcat agggttcata gacagggttg
    15541 agagaataaa gcaccagcct tccatccgtc atcagcctgt tttctatgat tttaaatact
    15601 attccgtcgt ttatcgtaaa cacaatgtat gcattgccgc tttttatttc gttccagtcc
    15661 tgaaggaact cacccgtaac ccatgacccg tcgggtatcg gcagcatcga gtcgcctttc
    15721 agctgaaagg tcctgtattt tctcctgtca gagagaaagg gaagcctgaa taccggaagc
    15781 tcgccgatat attccggatc ggcatagccg gttgtatagc cggcctttgc tttttcagca
    15841 acaagctcaa tgttttccct gttctcttta tccacagttg taaccagcac acggagattg
    15901 cttcccctga tgtatgcgtc gtagcctcgt tccagctcgc cgagctggct ttccgacagc
    15961 tttgtcatat cgaccttcag cagggtgtca accgacatgc tgaaatatcc cgaaaaagac
    16021 acaagtattt ctattccggg ctgagccaca ccgttctcat aaccgctgag cgttgaacgc
    16081 ttgagattta aggcagcggc aacttcatcc tgggtcctgc cccgcctttt ccgtaaaaat
    16141 tttatgttgg aagtaaagta catattgtaa aaatgattat attgtaatca tacttttgat
    16201 taatttttaa tcaaaagtaa aacattattc tgaaacaagc aaataatccg gaaaaaaaag
    16261 cccggaacag gttaaggttc atgagccgta gaatggcaaa acgaggaata cggcaaaacc
    16321 cttaatcccg ggctctttaa accgtattaa ccactttggg atgttaattg ccggcaacat
    16381 ggaaagatcg gttttgcatc tcgacctcga cacgtttttc gtgtcggtcg agaggctgaa
    16441 gaacagcagg cttatcggaa agcctgtgat cataggaggc ttgtcggacc gcagtgttgt
    16501 ttcgagctgc agctatgagg cccggaaata cggggtcaac tcggcaatgc cgatgaaaat
    16561 ggcaagaagc ctgtgtccgg aagccattat gatccgcggc gacatggaaa tgtacagcag
    16621 gtattcaaac atggttaccg aaataattgc tgaaaagtct cccctgtatg aaaaatcatc
    16681 catcgacgag cactatgtcg acctcaccgg catggatcgt ttcttcggat gctgcaaatg
    16741 ggcgcatgaa ctgaggcagc gcataatgaa agagacgggc ctgcctgtat cggcaggtct
    16801 ctcagtgaac aagacggtat ccaagattgc agccggggag gcaaagccga acggcgagat
    16861 tgaagtgggc agggagattg tcttcccctt tctcgatccg ctgccggtaa gcaggatccc
    16921 catggttggc ccgaaaacct gtcatattct ccgttcaatg ggtatcgcaa caatatatac
    16981 gctcaggaac attccggctg aaatggttga gaaggtactg gggaaaaacg ggattgaaat
    17041 atggaagaaa gccaacggaa ttgatccgtc gcctgtcatg agcttctccg aacaaaaatc
    17101 catcagcacg gagcatacgt tcgaaaagga caccaccgac atcgcacgcc tgaatcagct
    17161 gcttgcgggc atggtggaaa agatcgcgta tgatctgagg aaccaggaaa agctgacgtc
    17221 gtgcgtcacc gtgaagatca ggtattcaaa tttcgacacc cacaccctgc agaaaaggat
    17281 accgtacacc tctttcgacc atgtactcac tgatgttgcg aaagaactgc tttcaaggct
    17341 ttaccggcgc aggatgctga taagact
//
LOCUS       DAOOYG010000050        17077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3251_length_17077_cov_6.472154, whole genome shotgun sequence.
ACCESSION   DAOOYG010000050 DAOOYG010000000
VERSION     DAOOYG010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17077
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3251_length_17077_cov_6.472154"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..1781
                     /gene="guaB"
                     /locus_tag="PK288_06085"
     CDS             300..1781
                     /gene="guaB"
                     /locus_tag="PK288_06085"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPM18524.1"
                     /translation="MSFIKDKILFEGLTFDDVLLVPSYSEVLPREVELGSMFTRNIRI
                     NTPIVSAAMDTVTEDELAIAIAREGGIGVIHKNMPAEKQAALVKRVKRAENVMILDPI
                     TINLHASVAEAINLMSEYHIGGIPVVDDNGLLKGIVTNRDLRLENNYAKKITEVMTTK
                     LITTNHETDLEAAAKILQKHKIEKLPMIDESGRLIGLITYKDITKAKDRPNSCKDEKG
                     RLRVAAAVGIASDTLKRVEALIGANVDAIAIDTAHGHSKGVIRMLREIKKAFPGTDII
                     AGNIGTAEAARVLVDEGADALKVGIGPGSICTTRVIAGVGIPQLSAIYNVAKAVEGSG
                     VPVIADGGIRYSGDIVKALAAGADSIMAGSLFAGVEESPGETIIYSGRKFKTYRGMGS
                     IEAMSQGSKDRYFQDVEDDINKLVPEGIAARVPYKGLLSEVIYQMTGGLRAGMGYLGA
                     RNIAALKKEARFVRITNSGIIESHPHDVTITRESPNYSRKTFE"
     gene            1800..3767
                     /locus_tag="PK288_06090"
     CDS             1800..3767
                     /locus_tag="PK288_06090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM18525.1"
                     /translation="MKTRSILFATFILFAFPGTGVSQIIDEQILMTVNHREVPAGEFI
                     RMYIKSPAGSEKTAMDDYLEQYTIFKLKVADAIESGIDTTVAFRTELEGYRNQLAGSY
                     LTDPGLKEELMRKMYLRLLTEIRASHLLVKCPPDALPQDTLIAWNKAIELRNRILKGE
                     SFERIAAESSDDKSARRNGGNLGFFTAFQMVKPFEDAAFSLEPGGLSGPVRTSFGYHL
                     ILVTGKRPSMGRVKVAHIMKSVPENAGEKEISEAKAQIDSVYNMLEAGSPFGQLARLY
                     SDHTGSSSKNGELNWFGTGEMIPDFAEAAFAISDTGKYTAPVRTVYGFHIIKLLERKP
                     PPAFEEAMPLIESKISSAELDALQQQSLVRRLKKEYRFTLNKAVYEWFLKNTGPLIPT
                     GKSLYNQTHVPDGLIYTFADQAMTAEDFAVQLNNQDRFPANDPKVFIDHVLESVVSRH
                     ICEYEKSVLEKKYPDFRYLMNEFHDGILLFEISSDKVWDRAGTDTAGLFRYYEINKEK
                     HLSKRVLEGKIYSLSLPGGMKKLTNAYEKFRNNRDIDSRMAKRFNRGKAVPLTISEQA
                     WNAGEDQDIDGIEWKPGLQTFVWNKVPSLIFVKSIQEPSPLPFPAVQADLIPEYQDYL
                     MDDWIRQLKSKYTVEVNREIFEKVKKLLNDD"
     gene            3757..4614
                     /locus_tag="PK288_06095"
     CDS             3757..4614
                     /locus_tag="PK288_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18526.1"
                     /translation="MMIRIICYAVLILILNPGCRSNSPASRRIPLAKAGNTILYYDEI
                     PEQVTGLITAGDSNIVIQNYINSWAKRVLMYQKAEANLSSELKNEIEKQLDETRVNLI
                     VHEYQQKMMQAKMDTIITDNDLESYYAENENSFYLTSNIVKALFIKLPVETPGINRIK
                     QLYRSDNQKDMQDLEALCFQFAEKFDDFNEKWITMDRLMLELNEKITGQEAFLSRNNH
                     IEVTDSFSVYLIRIYDFRLRGSLAPFEYVRDDIKRIIWNNRRIGFIKNLETEIYNDAI
                     KENIFKTYR"
     gene            4614..5975
                     /locus_tag="PK288_06100"
     CDS             4614..5975
                     /locus_tag="PK288_06100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM18527.1"
                     /translation="MKNSLGLRSLIAICLFLASSACFSQVIVESIVAIVGNEVVYLSD
                     IENAVLDLRRNGDRTPIDELRCRVLHELLVSKLFLDQARIDSIEVTPDMVEGDLNMRI
                     NDAIRTAGSEKTLEDYFRKSMIEIRRDIRKSLMEQQIIMEVRSKIVKDLTITPADVRR
                     FYNSIPRDSLPVLPARVQISLIQLDPPDNEANKAEARQKLLDIRGEILAGKSFNVLAV
                     MYSEDPGSAANGGEIGFKLRGELEKEYADAAFSLAKNAISRIVETKYGFHLIQLIERK
                     GEMANTRHILIRPKVKPEEAERAISKLDSLARLIRKDSLKFEDVAYRFSTHKDSRING
                     GKFVSTNPSERINWFTLEEFNREMYTRIRDLKIGEISDPFRTTDEIGNVVFRIVRLDG
                     ETPAHRPDLKNDYQYLYAAALSSKQQESYREWIIKKIETTYIKIIDEFRDCRFLEEEG
                     WLK"
     gene            5963..7597
                     /locus_tag="PK288_06105"
     CDS             5963..7597
                     /locus_tag="PK288_06105"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HPM18528.1"
                     /translation="MAEMKLLRNTFGYVIFRISLPAFIFCSFPLDLLCQHVSGSQAGK
                     KKIEVIHSAVGIEELERLTGRRLTRLLGDVSLKHNDILMTCDSAHFYTGHNQTKAWGR
                     IHMQQGDTLHLYGDSLFYDGTTQAASVDGNVELIDQQTHLYTNSVKYDVANKIARYND
                     HGKIINGENTLTSITGVYYVSENLYHFKDSVKIVNPDYVMTADTMDYNSRSETAYFTG
                     PSELVGDSLYLYCEKGWYDTKNDISRIWKNSVIDNRQQVIRGDSMYYDGQAGFGQSFR
                     NTSITDTTNNIIVKGDYAWYSKTPEKFMVTDKALFIQASGNDSLFLHADTIFAVTRTT
                     AEGKNYRLVRAYFGTRIFSKNLQGKCDSLSYSFQDSVIRMYTLPVLWSDQNQLTADSM
                     ALFTVNRKADRLELYNSAFVAAQVDDIRFNQVKGRSLTGYFRDNELYKINIVGNAETI
                     YFLLDRDMVVGINNAKCARIEIFVSDGKITGINEYQSPEGTIDPPVVGNTETPKLEGF
                     MWLDKLRPKNIDDLFIKGLTDESEPEPEPDPNIHLH"
     gene            complement(7578..7799)
                     /locus_tag="PK288_06110"
     CDS             complement(7578..7799)
                     /locus_tag="PK288_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HPM18529.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDFAIRSGAPMEVIENLQEIE
                     DEGDIYESIEDIWPDYPSKDDFFFNEDEY"
     gene            complement(7894..8430)
                     /locus_tag="PK288_06115"
     CDS             complement(7894..8430)
                     /locus_tag="PK288_06115"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPM18530.1"
                     /translation="MKIYTRNGDDGLTSLTGKTRVPKYHPRIEACGSIDELIAWIGHL
                     LDFRENEGRKEMLLHIQDQLMRCAYLVASEDTIREEVMPDPCFLQKIENEIDRMEADL
                     PSLRNFILPGGHAAISGCHIARCVCRRAERSVVKLNFMEAIPEIVLKLLNRLADYLFV
                     LARRLGSDLDIQCVEWKV"
     gene            complement(8467..9177)
                     /locus_tag="PK288_06120"
     CDS             complement(8467..9177)
                     /locus_tag="PK288_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM18531.1"
                     /translation="MNKVIEIHDIVKNYQVGSVIVRALRSVSINIEKNEYVAIMGPSG
                     SGKSTLMNLLGCLDTPTGGTYILNGTDVSKMEDDYLAEIRNKEIGFVFQTFNLLPRYT
                     ALENVTLPLIYAGVPKAEREALAIETLHHVGLGDRMTHKPNELSGGQRQRVAIARALV
                     NKPSIILADEPTGNLDSKTSLDIMGLLDEIHRNGNTVILVTHEEDIANHAARIIRLLD
                     GTVHQDYINEKYKQKVVS"
     gene            complement(9250..9873)
                     /locus_tag="PK288_06125"
     CDS             complement(9250..9873)
                     /locus_tag="PK288_06125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM18532.1"
                     /translation="MKKDKRVINFRDYGSRTIKSEHDLRRVSDIAKHSPVSKKYGQLL
                     FRLSSAFGKQMIIEFGTSLGISTMYLAFSRPDSHVYTMEGCQEVSEIARENFREAGLS
                     NIILLTGRFEDLFTEIRTPGIRPGLVFIDGNHRKTPTLDYFNLVAGISGDETVVVLDD
                     IYHSPEMAEAWGIIRNDGRVTATIDIFKMGIVFFRRNITPGSYLVRY"
     gene            10164..12269
                     /gene="recG"
                     /locus_tag="PK288_06130"
     CDS             10164..12269
                     /gene="recG"
                     /locus_tag="PK288_06130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPM18533.1"
                     /translation="MEDFLDTNITYLSGVGPRRAELLGREAGIHTFRDLLYYFPYRYV
                     DRTRFYKIHELDPDLPYVQIRGSITGFSSEGYGAGRRLTADFRDETGTVRLVWFKGTR
                     WITSTYQPGVEYIVFGKPGIFNGMINIIHPEIDTAEKIEKSINAGLQAQYSTTENLKN
                     HYVTSKTISRLVESLLRQIRYRIPETLPSFLVSKYRLMDLHDALFKVHFPSGTDEMER
                     ARYRLKFEELFYIQLNLLRYKVNRNIRTKGFVFSNVGQYFNDFYHNYLPFSLTGAQKR
                     VIREIRKDLGSGRQMNRLLQGDVGSGKTLVALMCMLIAIDNGYQACLMAPTEILASQH
                     YQTILKLLGDIGLEVDLLTGSSTKKVRRDIDEKLRGGTLNILIGTHALIEENVRFRNL
                     GLVIIDEQHRFGVAQRAKLWEKSPDPPHVLVMTATPIPRTLAMTVYGDLDVSVIDELP
                     PGRIPVKTMHFTEPERNKVFGFIRNQISTGRQIFIVYPLIRESEKMDYRDLEDGWDTI
                     TRAFPAPEYCVSIVHGRMSTAEKDASMKLFKDGTAHILVATTVIEVGVDIPNATVMII
                     ESAERFGLSQLHQLRGRVGRGAEQSYCILMSSNKMSKEAAKRIEVMIRTNDGFEIAET
                     DLQLRGPGDIEGTLQSGIPFDLRIANLGKDGQIIEYVRNIAGEILANDPMLESSENLI
                     LRTELKRVFGTRQSWSNIS"
     gene            12386..12808
                     /locus_tag="PK288_06135"
     CDS             12386..12808
                     /locus_tag="PK288_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPM18534.1"
                     /translation="MKEAGITGTIEHEGTVQKVGSDSVTVNIHPVSACSGCHADGYCS
                     LSGRKEKTIRISGRYNVKPGEKVTVMMNESTGFRAVVLGYIVPLAVLVFSLAVLTAAS
                     VNELTAGIISVSVLVPYFLILYFTRHRIERNFLFTLKK"
     gene            12809..13675
                     /locus_tag="PK288_06140"
     CDS             12809..13675
                     /locus_tag="PK288_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPM18535.1"
                     /translation="MMGSTVIITIITLSLLALASSVILYFVAQRFRVFEDPRIDEVQA
                     ILPAANCGGCGFAGCRNFAEALVKAETFEGLNCPVGGATVMNEAARILGKVAEVVDPT
                     VAVLLCNGSPEFRPRTSRYNGASECRISHSLYLGDTDCSWGCLGDGDCVRSCSFGAMY
                     MDPVTMLPVIIDDKCVSCGMCVKACPRHLIELRKRAKKDRKIYVACQNCDKGGPARRA
                     CKVACIGCGKCVKVCAFDAITMENNLAYIDAVKCTFCRKCVVECPTNSILEINFPPRK
                     PKAEPQAEVAKV"
     gene            13700..15034
                     /gene="rsxC"
                     /locus_tag="PK288_06145"
     CDS             13700..15034
                     /gene="rsxC"
                     /locus_tag="PK288_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPM18536.1"
                     /translation="MLKTFPAGGVHPPENKITAGTPIKYLPVPENVIIPVTQHIGAPA
                     SPTVSKGDSVRAGQIIAAGKGFVSANVHSSVSGKVNKIDHIIDSTGFKQTAVFIDVEG
                     DEWVESIDRSSSILKETDLSPEDIIKKCLDSGIVGLGGATFPSHVKLTPVSGKHCEVL
                     IINGVECEPFLTSDHRLMLEKGEEIIAGIRIMMKALQVKKAMIGIENNKPDAIANLTK
                     LASAYCEISVHPLKVKYPQGAEKQLIRALIKREVPSGRLPLDVGAVVHNVGTVFAVYE
                     AVQKNKPLFERVVTITGKYLSNPGNYFVRTGTPLRHLIAAAGGQPENTGKIVNGGPMM
                     GKAIVNTDVPVVKGMSGVILFDEKESVRKTIEPCIRCAKCVSVCPLGLEPYLLMTLSE
                     RNLFEKAERERITDCMECGCCSFTCPANRPLLDYIRLGKSTVMKMIRERNIK"
     gene            15049..16050
                     /locus_tag="PK288_06150"
     CDS             15049..16050
                     /locus_tag="PK288_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPM18537.1"
                     /translation="MSNLLNVSQSPHIHGKESTQKLMLNVVIALMPALFTSVFYFGLG
                     AIIVTLTSVASCLLFEYLIQKFVLKKPISITDGSALVTGLLLAFNLPSNIPVFIIVIG
                     SFVSIGIAKMTFGGLGNNPFNPALVGRVFMLISFPVQMTSWPVPKGLGTGYADAVTGA
                     TPLAIIKEGLKNGESLSKLMGEIPSPAQMLMGDMGGSMGEVAAIALIIGFIYLLATRV
                     ITWHIPVSVTGSIAIFTTILWLANPEKNADPMFHLLAGGVLLGAIFMATDYVTSPMVP
                     KAMIIYGCGIGIITVVIRVWGAYPEGVSFAILIMNSFVPLMNAYIKPKRFGEEVTNG"
     gene            16043..16618
                     /locus_tag="PK288_06155"
     CDS             16043..16618
                     /locus_tag="PK288_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPM18538.1"
                     /translation="MAKTESTFKNMVISLTLISLVASACLGFVYEMTREPIELANLNK
                     KLSAIRQVVPEFTNNPNDEMYMLPTGDGDSLEIYPAKSGNEIVGYAVNTNTSTGFSGN
                     ISLMAGFKPDGTIINITVLEQKETPGLGTKMAEPAFKDQFKDKNPGNFILKVKNDGGP
                     VDAITAATISSRAFCHAVQRAYNALQKGGEK"
     gene            16615..>17077
                     /locus_tag="PK288_06160"
     CDS             16615..>17077
                     /locus_tag="PK288_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPM18539.1"
                     /translation="MNQLQNFTKGFVRENPILMLVLGCCPTLATTTSAVNGLGMGIAT
                     AFVLVGSNTVVAMLANLIPDKVRIPSFIIIIASFVTVVDLLMKAYVPDLYETLGIFIP
                     LIVVNCIVLGRAEAFASKNTVLSSAIDGLGMGLGFTMALGILGAFREILGSG"
BASE COUNT         4990 a         3728 c         4061 g         4298 t
ORIGIN      
        1 gccgcctgta ggcattaggc caagcgtatc cctgccgccg gcaacggact gtatgatctc
       61 ttcctggagt ggcctgaagg aagaataacc ccagtacttg acaagtatct gccggtgctg
      121 ttcaggattc atgaccgatt aattagccaa tgcaggaact cccccagggg catgcagtgt
      181 tccctgcaaa agtaataact gtatttcaat tttttatgaa ggttaaaaat aaagaaccag
      241 attcaggtat cctgtttttt tttgtaattt gcgagaccat tatttaaagc gatcagacaa
      301 tgtcatttat taaggataaa atcctctttg aaggcctgac tttcgacgat gtacttcttg
      361 tcccttcata ttctgaggta ttaccacggg aagttgagct gggttcaatg tttacccgca
      421 acatcaggat caacactcct attgtttcag cagcaatgga tactgttacc gaggatgagc
      481 ttgccattgc aattgccagg gaaggaggaa tcggtgtcat tcataaaaac atgccggccg
      541 aaaaacaggc agcactggta aagcgggtaa aaagagccga aaacgtgatg atactcgatc
      601 ctatcacaat caatctgcat gccagtgtgg ccgaggcgat caatctcatg agcgagtacc
      661 atataggagg aatcccggtt gttgacgaca acgggttact gaagggaatt gtcacgaaca
      721 gggatctgag actggaaaac aattatgcaa aaaagatcac tgaggtgatg acaacaaaac
      781 tgatcaccac caatcacgaa accgacctgg aagctgcagc aaagatcctg cagaagcaca
      841 agatagagaa actgccaatg atcgatgaat caggaagact tatcggacta ataacttaca
      901 aggacataac gaaggcaaag gaccggccaa actcctgcaa ggatgaaaaa ggaaggctga
      961 gggtggcagc tgcggtggga atagcctccg ataccctgaa acgcgtcgag gcacttatcg
     1021 gtgcaaatgt cgatgctatt gccatcgata ccgcccatgg ccattcaaaa ggagttatca
     1081 ggatgctgag ggaaataaag aaagctttcc cgggaaccga tatcattgca gggaatatag
     1141 gtaccgcgga agcagccagg gtacttgttg atgaaggagc agatgccctg aaggttggga
     1201 tcggaccggg atcgatatgt accaccaggg tgatagccgg cgtgggtata cctcagcttt
     1261 ctgccatata taatgtggca aaggcagtgg aaggctccgg ggtgcctgtt attgctgatg
     1321 gcggaataag gtattcggga gatatagtaa aagccctggc tgcaggtgct gattccatta
     1381 tggcaggatc actgtttgca ggagttgagg aatcacccgg ggaaaccatc atatacagcg
     1441 gccggaaatt caaaacttac cgcggtatgg gatcaatcga agcgatgtca cagggatcaa
     1501 aggaccgcta cttccaggat gtggaagatg atatcaataa acttgtcccg gaaggtattg
     1561 cagcacgggt tccgtacaag ggcctcctgt cggaggtgat ataccagatg acaggcggac
     1621 tgcgtgccgg tatgggttat ctcggagcaa ggaacattgc tgcattgaaa aaagaagcca
     1681 ggtttgtcag gatcacaaat tcaggaataa tcgagagcca tcctcacgat gtaactataa
     1741 ccagggagtc tcctaattac agcaggaaaa cattcgaata aggatcgcag gagaaccaga
     1801 tgaaaaccag atccatttta tttgcaacat tcatcctgtt tgcatttcca ggtacgggcg
     1861 tgtcgcagat aatcgacgaa caaatcctga tgacggttaa tcacagagag gtacctgcag
     1921 gcgagtttat caggatgtac ataaaaagtc cggcgggatc tgaaaaaacc gccatggatg
     1981 attacctgga acagtacacc atatttaaac tgaaggtagc cgatgcaatt gaatccggta
     2041 tcgatactac cgtggcattc cgcacggaac ttgaaggata caggaaccag cttgcgggta
     2101 gttatttaac tgatcccgga ttaaaggaag aactgatgag gaaaatgtac ctgaggctgc
     2161 tcactgaaat cagggcatca catttacttg taaaatgtcc gcccgatgct ttgccacagg
     2221 atacccttat cgcatggaac aaggcaatcg aactgcggaa caggatcctg aaaggagagt
     2281 ctttcgagag gattgcggct gaatcgtccg atgacaaatc ggcacggaga aacggcggaa
     2341 atctgggctt cttcactgcc ttccagatgg ttaaaccatt tgaagatgct gctttctcac
     2401 ttgaaccggg cggcctctca ggacctgtca gaacatcttt cggataccac ctgatacttg
     2461 ttaccggcaa acgaccatca atgggcaggg taaaggttgc tcatattatg aagtccgttc
     2521 ctgagaatgc cggggaaaaa gaaataagtg aagcaaaagc acagattgac tcggtttaca
     2581 acatgcttga agcgggcagt ccgttcggtc agctggcccg gctttattcg gatcataccg
     2641 gatcttcttc aaaaaacgga gaactgaact ggttcgggac aggggaaatg atcccggatt
     2701 ttgctgaagc agcatttgca atcagcgaca cgggaaagta tacagcccct gtaagaactg
     2761 tatacggatt tcatataatc aaactgcttg aaagaaaacc gccgcctgca tttgaagagg
     2821 ccatgccatt gattgaaagt aaaatcagct ccgctgaact tgatgcgctt caacagcaat
     2881 cgcttgtgcg gaggttgaag aaagaataca gattcaccct taataaggct gtttatgaat
     2941 ggttcctgaa aaataccggt ccgcttattc ccaccggaaa atccttatat aatcaaacac
     3001 acgttccgga tggattgatt tatacgtttg ccgaccaggc aatgacggca gaagattttg
     3061 ccgttcagct taataatcag gacagattcc ctgcaaatga tccgaaagtg ttcattgacc
     3121 atgtcctcga atcagtggta tcacgccata tctgtgaata tgagaaatct gttctcgaaa
     3181 agaaatatcc cgatttcaga tatctgatga atgagtttca cgacggaata ctgttatttg
     3241 aaatctcttc cgataaagtc tgggacaggg cgggtaccga cacagcagga ttattccgct
     3301 actacgaaat taataaggaa aaacaccttt caaaaagggt tctcgaggga aagatctatt
     3361 cgcttagtct gccgggaggc atgaaaaaac tcaccaacgc atatgaaaag ttcaggaata
     3421 atcgtgatat tgattccaga atggcaaaga gattcaaccg cgggaaggca gttccgctga
     3481 cgatcagcga acaagcatgg aacgcaggag aggaccagga tattgatgga atagaatgga
     3541 aacccggttt gcaaactttt gtctggaata aggttccttc acttatcttt gtaaaaagta
     3601 ttcaggagcc ttctcccctg ccgtttccgg cagtgcaggc tgatttgatt cctgaatatc
     3661 aggattatct tatggatgac tggataaggc aattaaaaag taaatacacc gttgaggtta
     3721 accgggaaat atttgaaaaa gttaagaaat tactgaatga tgattaggat tatctgctat
     3781 gctgtactta tcctcatcct caatccggga tgcaggagca acagtccggc ttcaaggaga
     3841 atacccctgg caaaggcagg gaacacgatc ctttattatg atgagatacc agaacaggtt
     3901 accggcctga ttactgccgg tgacagcaac attgtcattc agaactatat aaactcctgg
     3961 gccaaaaggg tactgatgta tcagaaagct gaagccaatt tatcttccga gcttaaaaat
     4021 gagatcgaaa agcagctgga tgaaacaagg gtgaacctta ttgtacacga gtatcagcaa
     4081 aagatgatgc aggcaaaaat ggataccata ataacggaca atgacctgga gagctattac
     4141 gctgagaacg aaaacagttt ttacctgacc tcgaacattg taaaggcact tttcataaaa
     4201 ttgcctgtgg aaactccggg aataaaccgg ataaaacagc tttacagatc agataaccag
     4261 aaagacatgc aggatctgga ggccctctgc tttcagtttg ccgagaagtt tgatgatttc
     4321 aatgagaaat ggatcaccat ggacaggctg atgcttgaac tcaatgaaaa aatcaccggc
     4381 caggaagcgt tcctcagcag aaacaaccat atagaagtta cggattcctt ctcggtctat
     4441 ctgataagga tatacgattt cagacttagg ggctcccttg cccctttcga atatgtcagg
     4501 gatgatatta aaaggatcat atggaataat cgcaggatcg gattcatcaa gaatcttgag
     4561 acagaaatat acaacgatgc gattaaggaa aatatattta agacctatag ataatgaaaa
     4621 acagtttagg attgcgttcc ctcatagcca tttgtttatt tcttgcttca tctgcatgct
     4681 tttcacaggt cattgttgaa tcaattgttg ccatagtagg aaatgaagtg gtttatcttt
     4741 cggatattga gaatgctgta ctcgatctga gaaggaacgg agacagaacg cccatagatg
     4801 agctcagatg cagggtcctg catgaattac ttgtttcaaa gctgtttctt gatcaggcca
     4861 ggattgacag catcgaggtc acccccgaca tggttgaggg cgatcttaac atgagaatta
     4921 atgatgctat cagaactgcc gggagcgaaa aaacactgga ggattacttc aggaaaagca
     4981 tgattgagat ccgacgcgat ataaggaaaa gccttatgga acaacagata ataatggagg
     5041 ttcgcagtaa aatagtcaag gatcttacaa ttacacctgc cgacgtcaga aggttctaca
     5101 attccattcc cagggacagt ctgccggtct tgccggccag ggttcagatc agccttatcc
     5161 agcttgatcc gcccgacaat gaagccaata aggcagaagc caggcagaag ctgcttgaca
     5221 taagaggcga gatactggcc ggcaagagtt tcaatgtgct ggcagtaatg tattcagagg
     5281 atccgggctc ggctgcaaac ggaggagaga tcggattcaa gctcagggga gagctcgaga
     5341 aagaatatgc cgatgctgct ttcagccttg caaaaaacgc aatctcaaga attgttgaaa
     5401 caaagtatgg gtttcacctt atacagctca tcgaacgcaa aggagaaatg gccaacacca
     5461 ggcatatttt gatcagaccc aaggtaaaac cggaagaagc tgaaagggcc atttcaaaac
     5521 tcgacagcct tgcaagactc atccgaaagg acagcctgaa attcgaggat gtggcataca
     5581 ggttctccac tcataaagac agccggatca atggtggaaa attcgtgagc accaatccgt
     5641 cggaaaggat aaactggttt acccttgagg aattcaacag ggaaatgtac accaggatca
     5701 gggatctcaa gatcggcgaa atatccgatc ctttcaggac cactgatgaa ataggcaatg
     5761 ttgttttcag gatcgtcagg cttgatgggg aaacgcccgc tcacagaccg gatcttaaaa
     5821 atgattacca gtatctgtac gctgctgctc tttcatcgaa acagcaggaa tcgtacaggg
     5881 aatggataat aaaaaaaatt gagaccactt atataaaaat aattgacgaa ttcagagact
     5941 gcagatttct ggaagaagaa ggatggctga aatgaagctg ctgcgcaata cctttggtta
     6001 tgtgattttc aggatctcct tgccggcttt tattttttgc tcgtttcccc tggatctgct
     6061 ttgccagcac gtatcaggtt cccaggcagg gaagaagaaa atagaagtga ttcattcggc
     6121 agtaggaatc gaggagctcg aaagactgac aggaagaagg ctcacaagac tattgggcga
     6181 tgtatccctg aagcataatg atatactaat gacctgcgac agcgcccatt tttacacagg
     6241 ccataatcag accaaagcct ggggaagaat ccatatgcag cagggcgata ctcttcacct
     6301 gtacggggat tcattatttt atgacggcac aacacaggct gcttccgttg acggcaacgt
     6361 agaacttatt gatcagcaga cacatctgta tacaaactcc gtgaaatatg atgtggccaa
     6421 taaaattgcc agatacaatg atcatggaaa aataatcaac ggggaaaata cccttacaag
     6481 catcacgggg gtttattatg tgtcggagaa cctgtatcac ttcaaggaca gcgtgaaaat
     6541 tgtcaatcct gactatgtga tgacagccga tacaatggac tataattcca ggagtgaaac
     6601 agcttatttc acaggtccgt cggaactcgt tggagacagc ctgtacctgt attgtgaaaa
     6661 gggatggtat gatactaaaa atgacatatc aaggatatgg aaaaactctg tcattgataa
     6721 cagacagcag gttatccggg gagactcaat gtactatgac ggccaggcag gattcggaca
     6781 gtcattcagg aacacaagca taacagatac tactaataat attatcgtaa agggtgatta
     6841 cgcatggtat tccaagacac ccgaaaaatt catggttacc gacaaggccc tgttcatcca
     6901 ggcatcagga aatgattctc ttttccttca tgcagataca atttttgcag ttacacggac
     6961 aactgctgaa ggaaagaact acaggcttgt gagagcctat ttcggcacca ggatattcag
     7021 caaaaacctg cagggcaaat gtgactccct gtcatattcg ttccaggatt cggtgatcag
     7081 aatgtatacc ttgcctgtcc tctggtcgga ccagaaccaa ttaaccgcag attcaatggc
     7141 actgttcacc gtaaaccgca aagctgacag gcttgaattg tataattcag catttgttgc
     7201 cgcacaggtt gacgatatca ggttcaacca ggtcaaaggc cggtcgctta cgggatattt
     7261 cagggataat gaactgtata aaataaacat tgtaggtaat gccgaaacaa tttatttcct
     7321 ccttgaccgc gacatggttg tgggcataaa caatgccaaa tgcgcacgta tcgagatctt
     7381 cgtgtcggat ggtaagatca ccggcataaa cgaataccag agtccggaag gaaccattga
     7441 tcccccggta gtgggaaaca ccgaaacgcc gaagctggaa ggatttatgt ggctcgacaa
     7501 actccgcccc aagaatattg atgacctatt tattaaggga ttaacagatg aatcagaacc
     7561 ggaaccggaa ccggatccta atattcatct tcattgaaga aaaaatcatc cttgctggga
     7621 taatccggcc atatatcttc tatgctctcg tagatatctc cctcatcttc tatttcctgc
     7681 aaattctcaa ttacttccat cggggcacct gatctgatgg caaaatcaat cagctcatcc
     7741 ttcgttgcgg gccacggggc atcctcaagt tttgatgcta attcaagtgt ccagtacatt
     7801 tccaaatgtt gtgttaaaaa gtgtgacaaa gtgtgcaaat atatatatta ttttatataa
     7861 aaaacaatac aatatttcta catatgcacc ttattatact ttccactcaa cacactgtat
     7921 atcaagatct gagcccagcc tgcgggccaa tacgaataaa taatcggcaa gccggttcag
     7981 taatttcagg actatttcag gaatcgcctc catgaaatta agttttacga ccgagcgttc
     8041 agcccttctg cagacacatc ttgctatatg acaaccggat atggctgcat gtccgcccgg
     8101 caggataaaa ttcctgagtg aaggcaagtc tgcttccatc ctgtcaatct cattttcaat
     8161 tttttgaagg aagcagggat cgggcataac ctcttccctg atagtgtctt cggaagccac
     8221 cagatatgca catctcatca gctgatcctg aatgtgaaga agcatttctt tcctgccttc
     8281 attctccctg aagtcaagca gatgaccgat ccatgcaatc agttcatcga ttgatccgca
     8341 ggcttctatc ctgggatgat actttggcac gcgggtcttt ccggtcaggg atgtgagtcc
     8401 gtcatcacca ttcctcgtgt atattttcat gtttttcagg tttagaaggc aacccgtgga
     8461 tctgaatcag gaaacaactt tctgcttgta tttctcattg atataatcct gatgaacagt
     8521 accatcaagc aggcgaatga tcctggcggc atgattggcg atatcttcct cgtgtgtgac
     8581 aaggataacg gtgttgccgt tcctgtgaat ttcatcaagc agtcccatga tgtcaagtga
     8641 tgttttgctg tcgaggtttc cggtgggctc gtcagcaaga attatcgaag gtttgtttac
     8701 gagggccctt gcaattgcaa ccctctgcct ttgtcctccc gatagttcat tcggcttatg
     8761 tgtcatcctg tcgcccaggc cgacgtgatg cagtgtttct attgcaagcg cttctctttc
     8821 agccttggga acacctgcat atatcagggg aagggtaaca ttctcaagtg cagtatacct
     8881 gggcagcaga ttgaatgtct gaaatacgaa tccaatttcc ttgttcctta tttctgcaag
     8941 ataatcgtcc tccatcttgc tcacatcagt cccgttcagt atatatgtcc cgcctgttgg
     9001 agtatcgaga cagccaagaa ggttcatcag ggtagatttc cctgatcctg aaggccccat
     9061 gatagctaca tattcattct tttcaatgtt tattgaaacc gaccttagag ccctgacaat
     9121 aactgatcca acctgataat tctttactat atcatgaatt tcaattacct tgttcataac
     9181 gattttttta ttaaggttcg aaattagatt aattaataca attctggaat tatatctgct
     9241 taatttttgt taatacctca ccaaatagct gcccggcgtg atattcctgc ggaaaaaaac
     9301 aattcccatc ttaaaaatat caattgtggc ggtaaccctg ccgtcattcc ttattatccc
     9361 ccatgcttca gccatctcgg gggaatggta tatatcatca agaaccacaa ccgtctcatc
     9421 gccggagatc ccggccacca ggttgaaata atcaagagtt ggggttttcc tgtgattacc
     9481 gtctataaag accaggccag gtctgattcc cggtgtcctg atctccgtaa agagatcttc
     9541 gaaacgaccg gttaaaagga taatattcga caatccggct tccctgaaat tctcacgggc
     9601 aatttcagat acttcctggc atccttccat cgtgtaaacg tgtgaatcag ggcgggaaaa
     9661 tgccaggtac attgtgctta ttcccaatga ggttccaaat tcgataatca tctgcttccc
     9721 gaaggcagaa gacagcctga aaagcaattg tccgtatttc tttgataccg gggagtgctt
     9781 tgcaatatcc gacactcttc tcagatcatg ttcgctcttt atagtccggg aaccataatc
     9841 cctaaaattt atcaccctct tgtctttctt catttttttt ctcagggttt caatcttaaa
     9901 gacaatttcc ggatcagttt tattctgaaa tattcctgaa accacattaa aaagaaaagg
     9961 gctgtgtatc ccatgcccct ttcggtggcc cgaactcagc agatatactg catatctggc
    10021 taaaggatag agattcactt ccccgtgaca ataaatgtat gacagttctc caaaaatagc
    10081 tattttcgaa tatccggttt gactgatcgt tcaaaataac ctttaatcag tcaaccgcta
    10141 atggatatca ggtgagtagt tccatggagg attttcttga cacaaacatc acctatcttt
    10201 cgggtgtagg gcccaggcgt gcggagcttc ttggcaggga ggcgggtatt catacattcc
    10261 gcgatcttct ttactatttc ccctacaggt atgtcgacag gaccaggttt tataaaatac
    10321 atgaacttga tcctgatctg ccttatgttc agatcagggg atcaataacc gggttttcat
    10381 ccgaaggtta tggtgcaggc aggcggctga cagctgattt tcgtgatgaa acaggaaccg
    10441 tcaggcttgt ctggttcaag ggtaccagat ggatcacttc aacgtaccag ccgggagtgg
    10501 aatacattgt tttcgggaag cccggaatat tcaacgggat gataaatatt atccatcctg
    10561 agatcgatac agcagaaaag attgaaaaaa gcattaatgc ggggctgcag gctcagtaca
    10621 gtacaacgga aaatcttaaa aaccactatg ttacatcaaa aaccataagc aggctggttg
    10681 aaagcctgct caggcagatc aggtaccgaa tccctgaaac cctgccttcc tttcttgtgt
    10741 caaaatatcg cctgatggat cttcatgatg ctcttttcaa ggtgcatttc ccgtcaggta
    10801 ccgatgagat ggaaagggcc aggtacaggc tgaagtttga agaacttttc tatatccagc
    10861 ttaatctttt gaggtacaag gtcaacagaa acattagaac caaaggattt gtattttcaa
    10921 acgtcgggca gtatttcaat gatttctatc ataactatct tcctttctca cttacgggcg
    10981 cccagaaaag ggtgatcagg gaaatcagga aagaccttgg gtcgggcagg cagatgaaca
    11041 ggctgctgca gggcgatgtg ggaagcggaa aaaccctggt tgccctgatg tgcatgctta
    11101 ttgccatcga caacggttac caggcatgcc ttatggcgcc cactgaaata cttgccagcc
    11161 agcactatca gacaatcctg aaacttctcg gggatatcgg tctggaggtg gatcttctca
    11221 cgggttcctc aacaaaaaaa gtacgcaggg acattgatga aaaactcagg ggaggtacac
    11281 tgaacattct tatcgggaca catgccctta tcgaggaaaa tgtcaggttc aggaatcttg
    11341 ggctggtgat tatcgatgaa cagcacagat tcggggttgc acaaagggcc aagctgtggg
    11401 aaaaaagccc tgatccgccc catgtgcttg taatgactgc cacgcccatt ccgcgtactc
    11461 ttgccatgac tgtttacggc gaccttgatg tgtcggtgat tgatgagctt ccgccgggcc
    11521 gtattcctgt aaaaaccatg cactttacag aacctgaaag gaacaaggta tttggattca
    11581 taaggaacca gatcagtaca ggcaggcaga tttttatcgt ctatcctctg atcagggaat
    11641 cggaaaagat ggattacagg gaccttgagg acggatggga taccatcaca agggctttcc
    11701 ccgctccgga atattgtgtg agcatagtgc acggccggat gagcaccgct gaaaaagatg
    11761 cctcgatgaa actattcaag gacggcacag cacatatcct ggttgcaaca acagttatag
    11821 aggttggtgt tgacatcccc aatgcaacgg taatgatcat tgaaagtgcc gagagatttg
    11881 gcctgtcgca gcttcaccag ttaaggggcc gggtcgggag gggcgcagaa cagtcatact
    11941 gtattctgat gagctccaac aaaatgtcga aggaagcagc aaaacggatt gaagtaatga
    12001 tcaggacaaa tgatggtttt gagattgcag aaaccgacct gcagctaagg ggcccgggtg
    12061 atatagaagg aactctccag agcggcatcc ctttcgatct ccggatcgca aatctcggaa
    12121 aagacgggca aataattgaa tatgtaagaa acattgcagg agagatactt gcaaatgatc
    12181 ccatgcttga aagcagcgaa aacctgattc tcaggacaga actaaaaagg gtcttcggta
    12241 cgagacaatc atggagtaac atcagttgat tcgattaatt agaatgattc taaataatta
    12301 tcaatgttcc aaaatctgac aaaaggcagt aactaatttt aaatgatttg taaatttgtc
    12361 ggcttcgtat ccactgtcat tgtttatgaa agaggctggt attacgggaa caatagaaca
    12421 tgaagggacg gtgcagaagg taggcagtga ttcagttact gtaaatattc atccggtatc
    12481 tgcctgttcc gggtgccatg cggatggcta ttgcagtctg tccggcagga aggaaaagac
    12541 aatcagaatt tcaggaagat ataatgtaaa gccaggagaa aaagttaccg ttatgatgaa
    12601 tgagtcaacg ggattcaggg cagtggtcct gggttatatt gttcctcttg cggtattggt
    12661 tttcagcctg gcagtactga ccgccgcatc tgttaatgaa ttaacagcag ggattatctc
    12721 ggtctctgtt cttgtgcctt actttttaat tctttatttt acaaggcacc ggattgagcg
    12781 caacttccta ttcaccttaa aaaagtaaat gatgggatca acggttatca taacaataat
    12841 cacgctgagt ctgctggctc tggcatcttc ggtgatcctg tattttgtgg ctcaaagatt
    12901 cagggttttc gaagatccga ggattgatga agtccaggcg atacttcccg ccgccaactg
    12961 tggcggatgc ggctttgccg gatgtcgcaa ctttgctgag gcgctggtaa aagccgaaac
    13021 atttgaagga ttgaactgtc cggtcggcgg ggcaacagta atgaatgaag ctgccaggat
    13081 cctcgggaag gtggcggagg ttgttgatcc gacagtggct gtactgcttt gcaacggcag
    13141 tcctgagttc cgtcccagga cttcaagata taatggagct tctgagtgca ggatatcgca
    13201 cagtttgtac ctgggagata cggattgcag ctggggatgc ctgggagacg gtgactgcgt
    13261 aagatcctgc agtttcggag ccatgtacat ggacccggta accatgcttc cggtaattat
    13321 cgacgacaaa tgcgtatcgt gcgggatgtg cgttaaggca tgtccgcggc atctgataga
    13381 gcttcgcaaa agagccaaaa aggacaggaa gatctatgtg gcctgccaga actgcgacaa
    13441 gggaggtccg gccaggagag catgcaaagt tgcatgcatt ggctgcggaa aatgcgttaa
    13501 ggtatgtgca ttcgatgcaa taaccatgga aaacaatctg gcttatatcg atgccgtaaa
    13561 atgcactttc tgcagaaagt gcgtggtgga atgccctaca aattcaatcc tggaaattaa
    13621 tttccctccc agaaaaccca aggcagaacc acaggctgaa gttgccaaag tataatttgt
    13681 tttataaaac atcagaattg tgttaaaaac atttcccgcc ggaggcgttc atccacccga
    13741 gaacaagata acagcaggca ctcccataaa gtatcttcct gtacctgaaa atgttattat
    13801 ccctgttacc cagcatatcg gtgcaccggc ctctcccacg gtcagcaagg gcgacagtgt
    13861 cagggccggt cagataattg cggcaggcaa gggatttgtt tcagcaaatg ttcattcatc
    13921 ggtatcaggt aaagtaaaca agattgacca tattattgac agcactggtt tcaaacagac
    13981 tgccgtattc attgatgttg agggtgatga atgggttgag tcgattgacc gaagcagcag
    14041 tatcctgaaa gaaaccgatc tttctcccga agatattata aaaaaatgcc tcgattcagg
    14101 catcgtcggc cttggagggg caacattccc ttcacatgtc aaactcaccc cggtatcggg
    14161 gaagcattgt gaggtcctta taattaacgg agtggaatgc gaaccatttc taacctccga
    14221 tcacaggctc atgctggaga aaggtgaaga gatcattgcc gggatccgta taatgatgaa
    14281 agcccttcag gtgaaaaagg caatgatcgg aattgagaac aacaagcccg atgcaattgc
    14341 aaatctcaca aagctggcat cagcctactg tgagatcagt gttcatccgc taaaggtaaa
    14401 ataccctcag ggtgcggaga aacaacttat cagggccctg ataaaaaggg aagtcccgtc
    14461 cggcaggctc cccctcgatg taggtgctgt tgtgcataat gtcggaaccg tttttgcggt
    14521 ttatgaagct gtccagaaaa acaagcccct ttttgaacgg gttgtcacca ttaccggtaa
    14581 atatctttcc aatcccggga attactttgt caggacagga acccccctta ggcatcttat
    14641 tgcggctgcg ggcggacagc ctgaaaatac cggaaagata gtgaacggcg gcccgatgat
    14701 gggcaaagcc attgtaaaca ccgatgttcc tgttgtaaag ggaatgtcag gcgtgatcct
    14761 ttttgatgaa aaagagtcgg tgagaaaaac catagagcca tgcatcagat gtgcaaaatg
    14821 tgtttccgta tgtccgctcg gcctcgagcc atatctgctg atgactttgt cggaaaggaa
    14881 tctgtttgaa aaagcggaga gagaacggat taccgactgc atggaatgcg gctgctgcag
    14941 ctttacatgt ccggcaaacc ggccgctgct tgattatatc aggctgggaa aatcgacagt
    15001 gatgaaaatg attcgtgaaa gaaacattaa ataggaatac cggaaaaaat gagcaacctg
    15061 ctgaatgtat cccagtcgcc ccatattcac gggaaggaat ccacacagaa actgatgctg
    15121 aatgtagtca ttgccctgat gccggcatta ttcacatctg tattctattt cggccttgga
    15181 gccattatag taacactgac ctcagtagcc tcatgtcttc tcttcgaata cttaattcag
    15241 aaattcgtcc tgaaaaagcc aatctcaata accgacgggt cggctctcgt aacgggactt
    15301 ctgcttgcat tcaaccttcc gtcaaatatt ccggtattca taatagtaat aggcagtttt
    15361 gtctccatag gaattgccaa aatgactttc ggcgggttag ggaacaatcc ttttaaccct
    15421 gcactggtgg gacgcgtttt catgctgatc tcatttcccg ttcagatgac ctcctggccg
    15481 gttcccaaag gcctgggcac cggttatgcc gatgccgtta ccggagctac accgctcgca
    15541 ataattaagg aaggcctgaa aaacggtgaa tccctttcca agcttatggg tgaaattcca
    15601 tctcccgcgc agatgctgat gggcgacatg ggaggatcga tgggcgaagt agccgccata
    15661 gctcttatca tcggatttat ttacctgctg gcaaccaggg taatcacatg gcacatacca
    15721 gtatcggtga caggttcaat agcaatcttc acaacgatcc tctggcttgc aaatcccgaa
    15781 aaaaatgctg atccgatgtt ccatcttctt gccggaggcg ttctgctcgg cgccatattc
    15841 atggcaaccg attacgtaac atccccgatg gttcccaaag cgatgatcat ctatggctgc
    15901 ggaatcggga taataacagt cgtaatacgt gtgtggggag catatcccga aggcgtttcc
    15961 tttgcaatcc tcataatgaa ttcttttgta ccgctgatga acgcatatat taagccaaaa
    16021 cgtttcggag aggaggtaac caatggctaa aaccgaatca acattcaaaa acatggtgat
    16081 atcgctcacc ctgatatcac tggtggcctc agcatgcctg ggctttgtat atgaaatgac
    16141 aagggagcct attgaacttg ccaatcttaa caaaaaactc agtgcaataa ggcaggtcgt
    16201 tcccgaattt accaataatc cgaatgacga aatgtacatg ctgcctacgg gagacggaga
    16261 cagccttgag atatacccag cgaaatccgg caatgaaatt gtcggttacg cggtaaacac
    16321 caatacttca accgggttca gcggcaatat cagtcttatg gccggattca aacccgatgg
    16381 tacgataatc aacattaccg tactggagca gaaagagact ccgggactgg gaacgaaaat
    16441 ggccgaacca gcctttaaag accagtttaa agataaaaat ccgggaaatt tcattctaaa
    16501 agtcaaaaat gacggagggc ctgttgatgc gatcacggcg gccacaataa gctcaagagc
    16561 attctgccat gcggtgcagc gtgcgtacaa tgctcttcag aaaggaggtg aaaaatgaat
    16621 cagcttcaaa acttcacaaa aggatttgtc agggagaatc ctatcctgat gctggtcctt
    16681 ggatgctgcc cgacactggc aacaaccaca tcggcagtta acgggctggg aatgggaatt
    16741 gcaacagcct ttgtgcttgt cggatcaaac actgttgtgg caatgcttgc aaacctgatc
    16801 cccgacaagg tcaggatccc gtcattcata attattattg cttcattcgt gaccgtggtg
    16861 gatctgctca tgaaggcata tgtacccgac ctttatgaaa cccttggtat ctttattccg
    16921 ctgatcgtag tcaactgtat agtacttggc agggcagaag cattcgcaag caagaatacc
    16981 gttctttcat cggctatcga cgggctgggg atgggtctcg gctttaccat ggcacttggc
    17041 atccttggag cattcaggga aatcctgggc agcggtt
//
LOCUS       DAOOYG010000051        16951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3291_length_16951_cov_7.303267, whole genome shotgun sequence.
ACCESSION   DAOOYG010000051 DAOOYG010000000
VERSION     DAOOYG010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16951
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3291_length_16951_cov_7.303267"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2140)
                     /locus_tag="PK288_06165"
     CDS             complement(<1..2140)
                     /locus_tag="PK288_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM18540.1"
                     /translation="MKKKLLLFLTLLTFIVSGVLAQDRTISGVVSSADDGSPLPGVNV
                     RVQGSTLGTITDAQGRYTLRVPSGFNTLEFSFIGMESRTETIGESNTINIAMESSSQV
                     LDEVVVTALGIERKVRAVGFAQQELKTDQLTAARESNLTNFLTAKVAGVKIAKTSSGT
                     GGSTAITIRGAKSLLGNNQPLFVLDGVPITNIGNTGGGTWEGIDLGDGMGDLNPEDIE
                     SMQVLKGPNASALYGARGSNGVILITTKSGRQKKGIGVEFNSNFSLETLNLFPTFQNY
                     YATGYEETNLYGEWVEIPKGSGKFYETMDTWHGDSWGPPMDGRRVIVDPFMFPDEEPY
                     SRTFTLLPQPEDNVRKFYNNGLGISNTLAISGSGEKTTARLSLGHTTNNGIIPNSKWE
                     KQTITINVNSKVTDYLSFDAKISYLRDEGNNRPILGTAYQSGNVSQVFASMGRYVPHD
                     FLREYYEKTKKPGNWPAVQYNPYYIVNELKSNDVKDRIIGQISTTLKIADWLSLMGRV
                     GTDYYSQKLVRKWPVGSLRSDNKEGRIYNELNLVKDLNADIILTASKELSSHFTGNLS
                     VGSSILYQERNNQTIDGRKFKAPGVYDISNCLDLRPSTYLSRKEMQSVFFTGQLAYNN
                     YLFLDVTGRNDWSSALGINNYSFFYPSVSTSFVFTDAFKSLSGNILSFGKIRASWAEV
                     GNDSDPYLTKNGYASTTVTFAGRPISWMNSR"
     gene            complement(2754..3479)
                     /locus_tag="PK288_06170"
     CDS             complement(2754..3479)
                     /locus_tag="PK288_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18541.1"
                     /translation="MRNPAVFSEENNLRNRINDISIGYPEIKGLLKRRPGENNVDFAV
                     RINKTVNDGFIHYWKDAGINKYHMRVPVWENYLLFAASYIDPEEYRKYEFSNYRKNLE
                     RGVGLCSTHSIVVKGVLKDNGIKAELLDVGGRHVVVRAELNDTATYLLDPDYGIVVPY
                     DTAAITANPELVRESYKNMADLYYPDAADPYTTDFMVKIFGDRKHVYSVDNWFEKFSY
                     AAIWILPVLLAMPSVFQITGKSG"
     gene            complement(3784..5262)
                     /locus_tag="PK288_06175"
     CDS             complement(3784..5262)
                     /locus_tag="PK288_06175"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HPM18542.1"
                     /translation="MKNKTKICVIICFSIISVSIPMGLQSQNPDHFLIIKPELINDVL
                     SNPGMGFMTFQRFNGDDLNAGPGWTEGFPIDYRDFNGNLTNKDYPATTIAYWRIYWKF
                     MEPEKGIYRFDMLDKALAIARSRGQTLLLRIAPYGTQSNNDVPDWYRKMVGPNRNWKY
                     NNPVNLWVVDPEDPRYAEHFGGLIRALGQRYDGHPDLEAVDLSIVGAWGEGAGSELLS
                     RDAREALVNAYTDSFRKTPIIALLMDRETNTYAASQIPVGWRIDCLGDLGFWAAEQNG
                     WSHMYDFYPREIINFNVSDAWIKSPVSFEICGTFLNWRDEQKYNREQVRYIFDQSLKW
                     HVSSFNAKSSPVPPEWSDLVDDWLRRMGYRFALRRFTCPESAEVNGKLEYTTWWENLG
                     VAPCYKDFAMAIRLRSGDRSEVFITGADVRNWLPGDIVYDDCIFVPWDFPTGPCDVQV
                     AIVDKLKHEPRVSLAIRGKQPDGWYQLGKVTITSDKLLLKDP"
     gene            5499..6521
                     /locus_tag="PK288_06180"
     CDS             5499..6521
                     /locus_tag="PK288_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18543.1"
                     /translation="MDNPDRKPNVAFQGFEDLGSPKFEHLKQKYMLDTVFHGETDEFK
                     RILLLRHWIKSVIAIDDHGDPYPGEGDVEAIIDNGLKGTGYHCGHYMIVQNAILNAYG
                     YVTRTLGAGPGVKGGPDGHHGIDEVWLNRYNKWFLSDNKYDHHFEKNGIPLSALEIRE
                     EFLKNRAADIIVVKGPGRVPTETDEETKISKERFAQAYTWIEWHGHNDIFTVWPAYTE
                     LLVMYNDDFFRNNVWIWDNKPHWAYSRPEFMKLVDNSDHIYWTPNTISSDVRIEGRQA
                     SIILDSDTPNLREYQVRTSPGGTWARTDPSFTLDLKKKKYELNFRVMNLAGVAGPEHR
                     IIIESK"
     gene            6615..8363
                     /locus_tag="PK288_06185"
     CDS             6615..8363
                     /locus_tag="PK288_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPM18544.1"
                     /translation="MNMNTSGRLLVSVLIAVVLKSFPGSGQISAQTGEYGIIEEIDVR
                     VPMRDGVRLSTNIYRPGAPGQYPVLLMRSPYGNGGSGNKTAIRAAGRGYAVVLQDTRG
                     RYESEGVFDAMQPEALDGYDTQQWIGAQSWCNGKIGTFGGSYVGFTQWMPAPLRSPYL
                     VTMFPTVTFSDLHDAIYQNGAFRYELFGLWSYEMTSPYNLSQDSLDARISRVLMSLPL
                     IDHDRKLGWKVPFLRDWLMHPEPDRYWDRTTIGDDYPKIKASVYAIGGWYDILLKGTI
                     DNYLKMTSPSVDPEIRKKQKLMIGPWVHDYGKRKVGELDFGEEADLNEEELMLRWFDN
                     QLRGVENGMDEEAPVKIFVMGANKWRFENEWPLARTVYQRYYLDSKGGANTRSGNGLL
                     DLKAPETGRSDTFVYDPSDPVPTTGSMGPYDLQKTEDRPDVLVYTSGPLKNDLEVTGP
                     VTAVVYASSSAMNTDFTARLTDVYPDGRSIRICEGIIRASHRNPDLPPTNIEPGKIYR
                     YNIDLWATSNVFKKGHRIRLEISSSNFPRFDRNLNTGTFFATETNWVKAEQRIFHGPD
                     YPSCIILPVITSSAAR"
     gene            complement(8495..9613)
                     /locus_tag="PK288_06190"
     CDS             complement(8495..9613)
                     /locus_tag="PK288_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM18545.1"
                     /translation="MKTLPKLKFSFVYPVIILSFFIAAQGCNQSGAAKNEYCTSADFY
                     TMPKIDVHCHVSVERPDFMELAIKDNFRILTINTDAAEEGPIEEQQRFALYQRNAFPG
                     RLAYLTTFSMKGWDEPDWQGRSLAYIKESFEKGAKGMKVWKNIGMVEKDRNNRFIMID
                     DPRFDTIFNYLEKNNIPVCGHIGEPKNCWLPLEEMTVNNDRKYFEEFPQYHMYLHPDY
                     PSYEDQVNSRDNLLKKHPDLHFMGAHLGSLEWDTDELAKRFDMFPNFNVDLAARICHI
                     QKQAIEDWKKVHDFFIKYQDRIMYGTDMGDFYGAKADPAEMKKRTHESWVRDWKFLTT
                     DSLMTSWEVNGEFRGLKLPRKVVEKIYHGNAEKMFPGI"
     gene            complement(9706..11937)
                     /locus_tag="PK288_06195"
     CDS             complement(9706..11937)
                     /locus_tag="PK288_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPM18546.1"
                     /translation="MRKIRNAFSFLAVAAFTLQAGGFGPGKTIITDSGHYSNVFGEFR
                     NFRIFLPPAYYENRGKRYPVIYFYHGWSQRYFGSISSEGYDEGKSNGGDNIAAFVETH
                     DVIVVKPDGYNRKPHEEYYLRPYNVSPVETFRQYPVYFPELVKFIDNNYRTIPDREHR
                     AVAGLSMGGFMAFWIGGKYPDLVTAAGSFCGSPEFYAGPQEFPAEYRHIDLAKNYKGV
                     NVRLNYGNRDFIRCYHRDLNRIWPNIMDNYQYKIYEAEHTTCGLGEMFGFIMQTFADP
                     PKKPLKWHHIDVYPSFEVWEYQVSSDRNLPGFTILENVDNRGFRSSVREFLPDGELLP
                     SVRLSVITAPVYDKNQLYTIRDFDLQTFSCTEKTIRSNESGRLKIDLDGGLHEIGINR
                     IRDKPNLCCGSVVIDGQPWVIAGREARIRLRILNKGNSEAVKVMAVVSAAGGNAAIRQ
                     NTASFGNIAPGNSSQGSDYLSFSVTADTIEMVKLKLMIRDGNRNEWSDQIEIAVRKDP
                     PAIENFEIADGRLFNVACSGTDTSALFLGNGNGDGIANPGESIVILVKDQGLFRRTEL
                     FSADQYINKNGINERHSDNWANYDHVGGSAKYSVPLISSDCPENHNIEFIASYWLPSY
                     PDHIIKYGKVSIPVSGQDNTSPEIRWINVTGDNTVQVKIRDGAGIRSARARFTSGGED
                     GRVFEADLNDEGRDGDRIKNDGLFSATVPKQNFGLFRMEVETIDIFGNRTIKKFDRLF
                     VFH"
     gene            complement(11996..13270)
                     /locus_tag="PK288_06200"
     CDS             complement(11996..13270)
                     /locus_tag="PK288_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPM18547.1"
                     /translation="MMKKIVLIICLGVLSGIPVIPQESPNYDESRVPSFSLPELLITR
                     EGKRISTAAEWMEIRRPEILSLFENNIYGRIPGKLSADFRKVIEQDNNALGGKAVRKQ
                     VMLNFRSNNRDLKVNLLIYLPKTGSKVPVFVGYNFDGNQAVVDDPAVFVTSSWIRNDP
                     ASGITNNRTTEKSRGTDKTAWQIDKIISSGYGLVTLYYGDVDPDKDDFTDGVHPLLYK
                     DGQTMPLPDEWGSIGAWAWGLSRVMDYLEKDPDIDASKVAVIGHSRLGKTALWAGALD
                     PRFAIVISNNSGCGGAALSRRMYGETLGGMNRYFTHWLCDNCSRYDNDVNLLPVDQHM
                     LIALMAPRPVYIASAEEDKWADPKGEYLSGYYATPVYELFGKQGLPSPEMPPVNSPVM
                     NTIGYHIRSGGHAVLEYDWDQFIRFARMHFGK"
     gene            complement(13531..15750)
                     /locus_tag="PK288_06205"
     CDS             complement(13531..15750)
                     /locus_tag="PK288_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18548.1"
                     /translation="MMFKLLYFHLTMLLITLIHCSELSGQFSPSAHRQPGPGEIPVSE
                     AGSYAIPGATYVLVNDITSPRSAVFLGKDVTLDLNGYTIAFADGNYGHVTNYGFEEGL
                     RGWDITKAPGAEIRNTKDTHAFIGKKLMSLKAGDELISDYVYLPLGERSYCAICGVTG
                     FYYDEMGGDLTRDMKVSIYVDDENGNGIQCVTRYSDSTKTGCPVLNRSPRLGGGFVFA
                     HLNNLPAGRYRVRVKAETDCLVDEADIRPAMDVGIGIVGQTHPMGHYDHLFNRDHSAF
                     FDYTADASDGTPVEGIPVLKGKGTVTIRNGTIRNAATGFLSWGIQSTAGDTRVILDNL
                     EIISSGINCTAVDVEQATITRCSFKIDNPFIINRHGAEFYAVDLRGKEASEVSFSSFL
                     GGQGCLSFKGDFSRIHHNTFMNRQTVTNHYSIMAMGDSSLIFSNYIEPETGSGIEVYI
                     HRGMEIFNNEFHISAAPPSCEYRDHLSTNGIRLADYGAKKGAPNGCFGNRVYNNRFFI
                     TGKKYHEYPGYIPMASAFFYSASGGDNEIFRNEIYIDQKDPGTDAEAFAFYIGNADGG
                     MIYSNNIISNVTPVWVGSAYGRAENTVLKGNTFERSPYAARNFMPVRMGSSESAECVA
                     DGIEFRSNSFKGLEFGVDATGQEHKYSVSWTLRINVRDRAGKPLNDRDVVITDRYGRE
                     SARLKSDIHGSVAAELAEFIQDGSQITRFSPYRISSGRKKMDIDLKNNTAADLIIRR"
     gene            complement(15791..16789)
                     /locus_tag="PK288_06210"
     CDS             complement(15791..16789)
                     /locus_tag="PK288_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HPM18549.1"
                     /translation="MFIPQSYSLAVVLCIVTMLCWGSWGNTQKLSGKSWRFELFYWDY
                     VIGILLFSLLLGFTLGSSGTQGRSFAADIGQAESANIFRAIAGGIIFNASNILLVAAM
                     AIAGMALAFPVGVGIALALGVIINYIFTPKGDPVILFAGVALIVTAIVIDAVAYKKHS
                     VSQQKVSGKGILLSVSAGVLMALFYRFVASSMATDFVNPEPGKLTPYGAVFFFALGVL
                     ASNFLFNYLIMKKPFEGKPLSFSDYRKGSAGVHLTGILGGVIWNLGMSMSILASGKAG
                     FAISYGLGQGATLIAALWGVFIWKEFRGASKMVNTLISLMFIAYLAGLILLVIAGK"
BASE COUNT         4335 a         4081 c         3884 g         4651 t
ORIGIN      
        1 tcctgctgtt catccatgag atcggccttc cggcaaaggt taccgttgtt gatgcatatc
       61 cgttcttggt aagatatgga tcggaatcgt ttccaacctc agcccaggaa gccctgatct
      121 ttccgaacga cagtatgttg ccggaaaggg atttgaatgc atccgtgaaa acaaagcttg
      181 tgctgacaga ggggtagaag aatgaatagt tgtttatccc aagcgccgac gaccagtcgt
      241 tcctccctgt cacatcaagg aagagatagt tgttgtatgc aagctggccg gtaaaaaaca
      301 cggattgcat ttctttcctc gataaatatg tgcttggccg gagatcaaga cagttgctta
      361 tgtcgtaaac accaggagcc ttaaatttcc ttccgtcgat ggtctgatta tttctttcct
      421 ggtacaaaat gcttgaaccg accgaaagat tgcccgtgaa atgacttgaa agctcctttg
      481 aggcagtaag aatgatatcc gcattcaggt cctttacaag attcagttca ttgtaaatcc
      541 ttccttcctt attatcgctt cttaatgaac caacaggcca tttcctgaca agtttctggg
      601 aataataatc ggtaccgacc cttcccatga ggctgagcca gtcagcaatc ttcagggttg
      661 tggagatctg tcctatgatc ctgtccttta cgtcgttgct ttttaattca tttacgatgt
      721 agtatggatt gtattgcacg gcgggccagt ttcctggctt ttttgtcttc tcgtaatact
      781 ctctcagaaa atcgtgcggc acatatcgtc ccattgaagc gaaaacctgt gaaacatttc
      841 cgctctggta cgcggtaccc aggatgggcc ggttattgcc ctcatcgcgc agataactga
      901 ttttagcatc aaatgacagg taatcggtta cttttgaatt gacattaatc gttattgtct
      961 gtttttccca tttggaattg ggtatgatac cattatttgt agtatgaccc agtgagagcc
     1021 ttgccgtagt tttttcgcct gacccggaaa ttgccagtgt gtttgatatc cccaggccat
     1081 tgttgtagaa tttcctgaca ttgtcttcag gctgcgggag cagggtgaag gttctcgagt
     1141 agggttcctc gtcggggaac atgaatggat ccacaattac ccttcttccg tccattggcg
     1201 gaccccagct gtctccgtgc catgtgtcca ttgtttcata aaatttaccg cttcccttgg
     1261 ggatttccac ccactcaccg taaaggtttg tttcctcgta tccggtggca taataattct
     1321 ggaacgtggg aaacaggttt aaggtttcaa gcgaaaagtt ggagttgaat tccacgccga
     1381 tacctttttt ctgccttccc gatttggtgg ttatcaggat gactccattg cttccccttg
     1441 ctccgtaaag ggcagaagcg ttcggaccct tcagaacctg cattgattca atgtcctcag
     1501 ggttgagatc acccataccg tcgcccaggt caattccttc ccaggtaccg ccgcctgtgt
     1561 tacctatgtt tgttatgggc acaccatcga ggacaaaaag cggctgattg tttccaagga
     1621 gtgattttgc tcccctgatg gtgattgcag ttgatccgcc ggttcctgaa gaagtttttg
     1681 caatctttac cccggcaacc ttggctgtca gaaaattagt gaggttgctt tccctggctg
     1741 cggtgagctg gtcagttttc agctcctgct gggcaaaccc tactgccctg accttccttt
     1801 caataccgag ggcggtaacg acaacctcat caagaacctg ggaggacgat tccatggcga
     1861 tattgatggt gtttgactca cctatagtct cggtcctgga ttccatcccg ataaatgaaa
     1921 actcaagcgt attgaagcct gaaggtaccc tcagggtgta acgcccctgg gcatcggtaa
     1981 ttgtacctaa tgttgagccc tgcaccctga cattcacgcc gggcagcggc gatccgtcat
     2041 cagctgagga taccacaccg cttatggtac ggtcctgcgc cagtaccccg ctcacaataa
     2101 aagttaacag ggttaaaaac agaagaagct tctttttcat agtggtagtt ttggtttatg
     2161 aattaaggac aaatctcaac ttttttatca acccctgatc ggagttggaa atattagctg
     2221 attattaatt taatattaaa taagtactag agcttgcgta ttcctatatt attgtataag
     2281 gtaatctttt gtttcattgt aaatatatta atatttttaa aaaaagtaag aaaaaaatca
     2341 tttttttaag attttaagaa aaaattataa tatgtaaatg gaaaaatgcg gttgttgtct
     2401 tctgtcttct catatcctgt atttgtaata atatcgcatt ccataccctt tcttaaggtt
     2461 gttgtcagcc tgccattcaa cacatacctg tccgggaaag gacaggatcc gggttcctgc
     2521 tcaatttttg caggcatggt gactgaagtt ctaccgttat cggatccgga agctcaggtg
     2581 atgagactgt agatcccagg catgattcat atgttttctt ttttaatgtt ttccttttgc
     2641 cactataagc acagccttac gggtatttca aaatcgtgct ggaaaaatta tccggcatca
     2701 tatcgtttta acagaggaat gcctgatgat taagacattg ctcccatgtt taatcagcct
     2761 gattttcctg tgatctgaaa tacagacggc attgccagca aaaccggtaa gatccagatt
     2821 gccgcgtaag aaaatttctc aaaccagtta tccaccgaat agacatgttt cctgtcgccg
     2881 aagatcttta ccataaaatc cgttgtataa gggtcagcag catcgggata gtacagatcg
     2941 gccatatttt tatatgattc ccttaccagt tcgggatttg cggtaattgc cgccgtgtcg
     3001 taaggtacaa caatgccgta atccggatca agcaggtatg ttgcagtgtc gttaagttct
     3061 gccctgacaa cgacatgccg cccgcccacg tcaagcagtt cggcctttat tccgttgtct
     3121 ttcagaacac cctttacaac gatggagtgg gtcgaacaca gccccacccc gcgttccagg
     3181 ttcttcctgt aattgctgaa ctcgtatttc ctgtactcct ccggatcaat gtaagatgcc
     3241 gcaaacaaaa gatagttttc ccagacaggg acccgcatat gatatttatt tattccggcg
     3301 tctttccagt aatgaatgaa tccgtcattt accgttttgt ttatcctgac agcgaaatca
     3361 acattgtttt ccccgggcct tcttttcaat aatcctttta tttcggggta tccgatactg
     3421 atatcattta tccggttcct gagattgttt tcctcggaaa agacagccgg atttctcatt
     3481 gtcatgaaca atccggtgat attgatgcag agtaaaatga taccggttaa gagaccgatt
     3541 ttatatagaa ctttcataac ctttcataat tggaaattta atcatcaata ttgccgtgct
     3601 caggttttta aacatcaata tggacttacc atattttaaa catggatgat tgaaatcatt
     3661 tttatttgtt ttggcatctg cagtctctgg gacttataaa aaaccaggta gatttatcag
     3721 aaaaccaaag ttcaaagaga taaatacttt atctggaatt gacaaatgaa tttgctgatt
     3781 taattaagga tctttcagta ataatttgtc cgacgttatt gtcactttac ccagctgata
     3841 ccagccgtca ggctgtttcc ccctgatggc caggctgaca cgcggctcat gcttaagctt
     3901 gtccactatt gcgacctgga catcacaggg gccagttggg aagtcccatg gtacgaaaat
     3961 gcagtcatca tatacaatgt cgccgggaag ccagttacga acatcggcac ccgtgatgaa
     4021 cacctcgctc cggtcgccgg acctaagcct gatcgccatg gcaaaatcct tgtaacacgg
     4081 agcaactcca agattttccc accaggtggt atattcaagc tttccgttga cttcagcgga
     4141 ttccggacag gtgaagcgcc tcagggcaaa cctgtatccc atcctgcgga gccagtcgtc
     4201 aaccaggtcg gaccattcgg gaggaacagg agatgatttg gcgttaaatg aggatacgtg
     4261 ccacttcaat gactggtcaa atatgtatct gacctgctcc ctgttgtatt tctgttcatc
     4321 ccgccagttg agaaaagtgc cgcagatttc aaagcttacg ggcgatttta tccaggcatc
     4381 gcttacattg aagttgatta tttcgcgggg ataaaagtca tacatgtgcg accatccgtt
     4441 ctgttccgct gcccagaacc caaggtcgcc gagacaatcg atccgccatc ccaccgggat
     4501 ctgcgatgcg gcataagtat tggtctccct gtccatcaga agggctatta tcggggtctt
     4561 tctgaaggaa tcagtgtagg catttaccag cgcctccctt gcatccctgc tgagcagctc
     4621 agagccggca ccttcccccc aggcacccac aatcgaaaga tcgaccgctt cgagatcggg
     4681 atgaccgtca tacctttgtc ccagtgccct gatcagaccg ccaaagtgtt ctgcatatct
     4741 gggatcttcc ggatcaacaa cccacagatt gacgggatta ttatacttcc agttcctgtt
     4801 tggacctacc attttcctgt accagtccgg aacatcatta ttgctttgcg tgccgtatgg
     4861 agcaatgcgc agcagcagtg tctggcccct ggaacgggct atggcaagtg ccttgtcgag
     4921 catgtcaaac ctgtagatgc ctttttcagg ctccatgaac ttccagtata tccgccagta
     4981 agcaatcgtt gtggccggat aatccttgtt tgtcagattg ccattgaaat cccggtaatc
     5041 gatgggaaag ccttccgtcc agcccggccc cgcgttgaga tcgtctccgt tgaaacgctg
     5101 gaatgtcata aatcccatgc cgggatttga aaggacgtca ttgatcaatt ccggcttaat
     5161 gatcaaaaaa tgatcgggat tctgcgattg cagccccatt ggtatggaaa cagaaataat
     5221 tgaaaagcaa ataatgacac agatttttgt tttgttcttc attgcagttt ttttgacagg
     5281 ttgtggaaat aattaacttt gttaatgttt tgcaacaaaa ttaaaaactt gatcagataa
     5341 atatattgaa aattttgtaa acacaaggat gtgttaattt attaacagta aatatttatt
     5401 attatcagaa ttataagaat atgaaattaa gaatactgtt attattttta atattttgtt
     5461 ctttttcttg tggtaagaga acttattaca ttgctgaggt ggacaatcca gatcgtaaac
     5521 ccaatgttgc ctttcagggt ttcgaggacc tgggatcacc gaagtttgag caccttaaac
     5581 agaaatacat gcttgacact gtgttccacg gggagacaga tgaatttaag agaatattgc
     5641 ttctccgcca ctggataaaa tcagtgatcg cgatcgacga tcatggcgac ccctatccgg
     5701 gcgaaggcga tgttgaagcc attatcgata acggcctgaa aggaacaggc tatcattgcg
     5761 gacattatat gattgtgcag aatgccattc tcaatgctta tggctatgtc acacgtaccc
     5821 ttggcgccgg accaggcgtc aaaggaggcc ctgacggtca tcacggtatc gatgaagtat
     5881 ggctcaaccg gtacaataag tggttccttt cagataacaa atacgatcac cattttgaga
     5941 aaaatggaat acccctctcg gctctcgaaa taagggagga attcctgaag aacagggctg
     6001 cggatatcat tgtggttaag ggaccgggac gcgttccgac cgaaaccgat gaagaaacaa
     6061 agatcagcaa ggagcggttt gcccaggcat atacctggat tgaatggcac ggacacaacg
     6121 atatctttac cgtctggccc gcttacaccg agctgctggt aatgtacaat gacgatttct
     6181 tcaggaacaa tgtatggatt tgggacaata aacctcattg ggcatacagc cgcccggagt
     6241 tcatgaagct tgttgacaat tccgatcata tttactggac gccgaatacg atttcttccg
     6301 atgtcaggat tgagggcaga caggcatcca taatactgga ttccgatact ccgaatctga
     6361 gggaatacca ggtacgaacc tcgcccggag gaacatgggc ccgaacggat ccttcattca
     6421 cacttgacct gaaaaagaaa aaatatgaac tcaatttcag agttatgaat cttgcaggag
     6481 ttgcgggacc agaacacagg ataattattg aaagtaagta aaaaagcgtt ccggacataa
     6541 gctgccgcct gtctggaaaa gccgtataat atataagttg tattcattta taatcattaa
     6601 cttaatttct tattatgaat atgaatacct caggcaggtt attggtgtcc gttttaatag
     6661 cagttgttct taaatcattt ccgggttcgg gacaaatttc cgcccagaca ggcgaatatg
     6721 gaattattga ggagattgat gtcagggttc cgatgaggga cggagtaagg ctttcaacca
     6781 atatttaccg gcccggcgcc cccggtcagt acccggtgtt gctcatgcga tcgccgtacg
     6841 gcaacggcgg gtcagggaac aaaactgcca ttagggcagc gggcaggggc tacgcggttg
     6901 ttctccagga tacaaggggg agatatgaat cagaaggtgt atttgacgca atgcagcccg
     6961 aggccctgga cggatacgac acccagcaat ggattggagc ccagtcgtgg tgcaacggga
     7021 agatcggcac tttcggcgga tcctatgtcg gcttcaccca gtggatgcct gcacccctcc
     7081 ggagccctta tctggtaaca atgttcccga cagtcacttt ttccgatctt cacgatgcga
     7141 tctaccagaa cggagctttc aggtacgaac tgttcggatt gtggagttat gaaatgacat
     7201 caccgtataa tctgagccag gattcccttg atgccagaat tagcagggta cttatgagcc
     7261 tgccgttgat tgaccatgac agaaaattag gctggaaggt ccctttcctc cgcgactggc
     7321 tgatgcaccc ggagcctgac aggtattggg accggacaac catcggggat gattacccga
     7381 agataaaggc atcagtctat gcaatcggag gctggtacga catactgctg aaagggacaa
     7441 tagataatta ccttaaaatg acctctccat ccgttgatcc ggaaatcagg aaaaagcaga
     7501 aactcatgat cggcccctgg gttcacgatt acggaaagcg caaggtgggg gagctggatt
     7561 ttggtgaaga ggctgatctg aatgaagaag aactgatgct ccgatggttc gacaaccagc
     7621 tcaggggagt cgaaaacggt atggatgagg aagcgccggt aaagattttt gtcatgggag
     7681 caaataaatg gaggtttgaa aatgaatggc ccctggccag gactgtttac cagcgctatt
     7741 atctggatag caagggcggt gcaaacaccc ggtcggggaa tgggctgctt gatttaaaag
     7801 cacctgaaac gggcagatcc gacacttttg tatatgatcc ttccgatccg gtaccgacaa
     7861 caggcagcat gggaccctat gacctgcaaa aaaccgaaga ccgcccggat gtcctggttt
     7921 atacctcagg cccccttaaa aacgatcttg aggtcacggg acccgttaca gctgttgttt
     7981 atgcttcaag ttccgccatg aacacagatt ttacagccag gcttaccgat gtttaccccg
     8041 atggaaggtc aataaggatc tgtgagggga tcatcagggc ttcccacagg aatcctgatc
     8101 tgccgccaac aaatatcgaa cccggcaaaa tctacagata taatattgat ctctgggcaa
     8161 ccagcaatgt atttaaaaag ggccacagga taaggctgga gatatccagc agtaatttcc
     8221 ccaggtttga cagaaatctt aataccggaa ctttttttgc aaccgaaaca aactgggtca
     8281 aagccgaaca aaggatattt cacgggccgg attatccgtc gtgtatcata ttgcctgtta
     8341 taaccagctc cgctgcaaga taagaagaag tatccatgcc ccgggtgctg accttcagca
     8401 agtgcggccg gtggtttatt cgggcattct gctccggaag tcgggacggc gccagatccg
     8461 gaggctgaaa atgttccggg acctgatacg ggctttaaat tcccgggaac attttttcag
     8521 cattcccgtg atatattttc tcaaccactt tacgtggcag tttaagtccc ctgaattcac
     8581 cgttcacttc ccatgaagtc atcagggaat cggtcgtcag gaatttccag tctcttaccc
     8641 atgattcgtg ggtccttttc ttcatttctg ccggatctgc tttggccccg taaaagtcgc
     8701 ccatgtcggt tccgtacatg atcctgtcct ggtatttaat gaaaaaatca tggactttct
     8761 tccagtcttc aatcgcctgc ttctggatat gacaaatccg ggcagccagg tcaacgttga
     8821 agtttggaaa catgtcaaaa cgcttagcca gttcatctgt atcccattca aggctgccga
     8881 gatgggctcc catgaaatga agatcaggat gtttcttcaa aagattatcc cttgagttga
     8941 cctgatcctc ataggatgga taatcgggat ggaggtacat atgatactgc ggaaactcct
     9001 caaaatattt cctgtcgttg ttcactgtca tttcctccaa tggcagccag cagttctttg
     9061 gttctccgat atggccgcag acaggaatat tgttcttttc aagatagttg aatatggtgt
     9121 cgaaccgcgg atcgtcgatc attatgaacc ggttgttcct gtccttttcc accatgccga
     9181 tgttcttcca tactttcatg ccttttgctc ccttttcaaa ggattctttt atgtaggcaa
     9241 ggcttctgcc ctgccagtct ggttcatccc atcccttcat cgaaaatgtg gtcaggtatg
     9301 ccagcctgcc tgggaaagca ttgcgctggt acagggcaaa tctttgctgt tcttcaatcg
     9361 gtccttcctc tgcggcatcg gtattgatgg tgagtatcct gaaattatct tttattgcca
     9421 gttccatgaa atcgggcctt tcaacactaa catggcaatg tacgtctatt ttcggcatgg
     9481 tgtaaaaatc agcgctggtg caatattcat tctttgctgc ccctgactgg ttgcagccct
     9541 gggcggcgat gaaaaaagaa agtattataa ctggatagac aaaactaaac ttcaattttg
     9601 gtaatgtttt catgatcatt ggtttttaaa tcactatttc cttatccagc catcgtaaaa
     9661 cacatcccgg gaaacaccac tgaatggttg ttttaattga tccggtcagt gaaatacgaa
     9721 aagcctgtca aattttttaa tggttctgtt tccaaagata tcaattgttt ccacttccat
     9781 cctgaacaat ccgaaatttt gttttggtac ggttgcgctg aacaaaccgt cgttttttat
     9841 cctgtcaccg tctctccctt catcattcag atctgcctcg aaaacccttc catcttcacc
     9901 gcctgaagta aaccgggctc ttgccgacct gatcccggcg ccatccctga ttttaacctg
     9961 caccgtattg tctcctgtca catttatcca tcttatttcg ggggacgtgt tgtcctggcc
    10021 ggaaaccggg atggagactt tgccatattt tattatatga tcagggtagg aggggagcca
    10081 gtaagaagct atgaattcaa tattatgatt ttcagggcaa tccgatgaaa tcagcggcac
    10141 cgagtatttg gccgaaccgc cgacgtggtc atagttggcc cagttatcgc tgtgtctttc
    10201 atttatgcca ttcttattaa tgtattgatc tgctgaaaac aattcggtcc gcctgaaaag
    10261 tccctggtct ttcacaagaa ttactatcga ttcaccgggg ttggcaattc cgtccccgtt
    10321 cccgtttccc aggaaaagtg ctgacgtatc tgttcccgaa catgccacat tgaaaagccg
    10381 gccatcggca atttcaaaat tctcaattgc cgggggatct ttccttaccg cgatctcaat
    10441 ttgatctgac cattcattcc tgttcccgtc cctgatcata agttttagtt ttaccatctc
    10501 aatggtatca gcagtcaccg agaaggataa ataatcgctt ccctggctgc tgtttcccgg
    10561 cgcaatgtta ccgaaagatg cagtattctg acggatagcc gcattgccac cggccgccga
    10621 cacaacagcc attaccttca ctgcttcaga gttgcccttg ttcagaatgc gtagtctgat
    10681 cctggcttcc ctgccggcaa taacccaagg ctgaccgtca atcacaacac tgccgcaaca
    10741 cagatttggt ttgtccctga tcctgtttat ccctatctca tgcagcccgc cgtcgagatc
    10801 gatcttcagc cggccggatt cattgctgcg aatggttttt tcagtgcagg aaaaggtttg
    10861 aagatcaaaa tccctgatgg tgtaaagctg gttcttgtca tatacggggg ccgtgatcac
    10921 actcagcctg acggagggca gaagttcgcc gtcgggcagg aattcccgta ccgagcttct
    10981 gaatcccctg ttatccacgt tctcaagaat ggtgaaaccg ggcaggttac ggtcggaact
    11041 gacctggtat tcccagactt caaatgaggg atacacatca atgtggtgcc atttcagggg
    11101 ctttttaggc ggatcggcaa atgtctgcat gatgaatccg aacatttcac cgagtccgca
    11161 tgtggtatgt tcagcttcat atattttgta ctgataattg tccatgatgt ttggccagat
    11221 cctgttaaga tcacggtgat agcagcggat aaaatccctg ttgccgtaat tcagcctgac
    11281 gttgactccc ttgtagtttt ttgccaggtc aatgtgcctg tattcggcag gaaactcctg
    11341 cggaccggca taaaattcgg gcgacccgca gaaactgccg gctgccgtta ccaggtccgg
    11401 atacttgccg ccgatccaga atgccatgaa cccgcccatg gagagccccg caactgccct
    11461 gtgctccctg tcaggaatgg tcctgtagtt attgtcgata aatttcacaa gttcagggaa
    11521 gtaaacggga tactggcgaa aagtctcaac cggtgagaca ttgtatggcc gcaggtagta
    11581 ttcctcatgc ggtttccggt tatacccgtc gggtttgaca acaatcacgt catgggtttc
    11641 aacaaatgcg gcaatattgt cgccgccgtt gctttttccc tcatcgtagc cttccgagct
    11701 tatgcttccg aaataccttt gcgaccagcc atgataaaag tagattacag gatatctttt
    11761 accacgattt tcataatatg ccggcggcag gaatatcctg aagttcctga actccccgaa
    11821 cacattgctg taatgacccg aatcggtaat tattgtttta cccggtccga agccgcctgc
    11881 ctgaagcgta aaagcagcaa ctgcaaggaa actgaaggca tttctgattt ttcgcatggt
    11941 tatatatgat aaggagaatt tggggcgcag ctattctgaa caggtggcca gtggtttatt
    12001 ttccgaaatg catccgggca aagcgtatga actgatccca gtcatactca aggaccgcat
    12061 ggcctccgct gcggatgtgg tagccgattg tgttcatcac cggtgaattg accgggggca
    12121 tttcagggga gggaagtccc tgtttcccga aaagctcgta aacgggtgtg gcataatagc
    12181 ctgaaagata ttctcccttc ggatccgccc atttgtcctc ttcggcactt gcaatataaa
    12241 cgggccgcgg agccatcagg gcaataagca tatgctgatc gaccggcaga aggttgacat
    12301 cgttgtcata cctgctgcaa ttgtcacaca gccagtgggt gaaataccgg ttcatcccgc
    12361 cgagggtttc tccgtacatc ctgcgcgaga gagctgctcc cccgcagccg gaattatttg
    12421 agatgacgat ggcaaacctc ggatcgagcg cccctgccca caaggccgtc ttgccgagcc
    12481 gcgaatgacc aattaccgcc accttcgatg cgtcaatgtc aggatccttt tccaggtaat
    12541 ccataacccg gctcaatccc catgcccagg caccaattga gccccactca tccggaagag
    12601 gcatggtctg accatcctta tacaggagag ggtgtacacc atccgtaaaa tcgtccttgt
    12661 cgggatccac atccccgtaa taaagggtga ccagaccgta tcccgatgag ataattttat
    12721 caatctgcca ggctgtttta tccgttcccc tcgatttttc agtggtgcgg ttgttggtta
    12781 taccggatgc cggatcattg cggatccacg aactcgtgac aaaaaccgcc ggatcatcaa
    12841 caaccgcctg gttaccatcg aaattataac ccacaaaaac gggaacctta ctgccggtct
    12901 tcgggagata aataaggaga ttgaccttaa gatcccggtt gttgctgcgg aaattcagca
    12961 taacctgttt cctcacggct tttcctccca gggcattgtt gtcctgctcg atcaccttcc
    13021 ggaaatcagc tgaaagttta ccggggatcc tgccatagat gttgttctca aacagcgaca
    13081 ggatctccgg ccgcctgatt tccatccatt cggctgcggt ggatatgcgt ttcccttccc
    13141 tggtaatcag aagttcggga agactgaatg acggaaccct gctctcgtca taattcggac
    13201 tttcctgcgg aatgaccgga ataccggaca gtacgccgag gcagatgatt aatacgatct
    13261 ttttcatcat aatataattt tgattttaat ggtatgatgc ccgcctgacc cgtcgggcag
    13321 ggaaccgcac atgattgact ttgtcgagct cgcaagctcg gttgaaaatt atattcttat
    13381 cgtgtttgtg attgtggtca atcatgtggg tttcatgtga aaattatttt gattatacag
    13441 accctgttca ttttatcaaa gcctgataaa tattccgttc caggcttata ggcagcaggc
    13501 ggacagcttc cacggacaaa ttcctgacag ttacctcctg attataagat ccgctgccgt
    13561 attattcttc agatcaatat ccattttttt cctgcctgaa cttatcctgt agggcgagaa
    13621 cctggtgatc tggctgccat cctgaatgaa ttcagccagt tcggctgcaa ctgatccgtg
    13681 tatatcgctc ttcagacgtg ccgattccct cccgtacctg tcggtgatga ccacatcccg
    13741 atcattaagg ggtttgccgg ccctgtccct gacatttatc ctgagtgtcc aggaaactga
    13801 gtatttatgc tcctggccgg tcgcatcgac cccaaactcc agtcccttaa aactgtttga
    13861 cctgaattca atcccgtcgg caacacattc ggcactttcg gacgatccca tcctcaccgg
    13921 catgaaattc cgggcggcat agggagatct ttcaaaagtg tttcctttca ataccgtatt
    13981 ttctgccctt ccgtaagctg atcccaccca taccggtgta acattggata ttatgttgtt
    14041 gctgtatatc atacccccgt cagcattgcc gatgtagaaa gcaaaggcct cggcatctgt
    14101 gccgggatcc ttctggtcaa tatagatctc attcctgaag atttcattgt cgcccccgct
    14161 ggcactgtaa aagaacgctg aagccatggg tatgtaaccg ggatattcat gatatttctt
    14221 tcctgtgatg aagaacctgt tgttgtaaac cctgtttccg aagcaaccgt tcggtgcccc
    14281 tttttttgcc ccgtaatcag ccagccttat tccgttagtg ctgagatggt cgcgatattc
    14341 acagctggga ggtgccgcac tgatatggaa ttcattgttg aagatctcca tgccgcggtg
    14401 tatgtaaacc tcaatccccg aaccggtttc gggttctatg tagtttgaga atatcaggga
    14461 gctgtcgccc atcgccatga tgctgtaatg atttgtcact gtctggcggt tcataaaagt
    14521 attatgatga atccttgaga aatctccctt gaatgaaaga catccctgcc ctcccaggaa
    14581 agaggaaaaa gaaacctctg atgcctcctt acccctaaga tcaacggcat agaattcagc
    14641 tccgtgacgg ttgatgataa acgggttatc aattttaaaa gagcatcttg ttatggttgc
    14701 ctgttcaaca tcgacggcag tgcagttaat tcctgatgat attatttcca gattgtcgag
    14761 gattacccgg gtatctccgg ctgttgactg aattccccat gagaggaaac cggttgcagc
    14821 gttccttatg gttccgttcc tgatggtaac ggttcctttt cctttcaaaa ccggtatgcc
    14881 ttccacgggt gttccatccg atgcatcggc tgtgtaatcg aagaaagccg aatgatccct
    14941 gttgaacaga tgatcgtaat gccccatggg atgggtttgt ccgacgatgc ctattcccac
    15001 gtccattgcc ggccttatat ctgcttcatc caccaggcag tcggtttcgg ctttcacacg
    15061 gacacggtac cggccggcag gaagattatt aaggtgtgca aacacgaaac cgcctccgag
    15121 tcttggcgac cggttaagaa ccgggcagcc cgttttcgtt gaatcactgt agcgggtaac
    15181 gcattggatc ccgttgccgt tttcgtcatc cacatatatg ctgaccttca tgtccctggt
    15241 gagatctcct cccatttcat cataataaaa gcctgttacg ccgcagatgg cgcagtaaga
    15301 cctttcaccc agcggcagat aaacataatc ggaaatcagt tcatccccgg ctttcaggct
    15361 catcagcttt tttcctatga aggcatgggt gtctttggta ttccttattt cagcccccgg
    15421 tgccttcgta atatcccagc ccctaagtcc ttcctcaaat ccgtaattgg ttacatgtcc
    15481 gtaattcccg tcagcgaatg caatagtgta gccgttgagg tcgagagtaa catccttgcc
    15541 gagaaaaaca gcactccggg gtgaagtaat gtcatttacc agaacataag tggcaccggg
    15601 gatcgcgtaa cttccggctt cgctgacagg aatctcgccc ggaccgggct gcctgtgggc
    15661 ggaaggagag aattgtcctg acagttcgga gcaatggatc aatgttatca gcaacattgt
    15721 caggtgaaaa taaagcagct taaacatcat tttgcagact gattcagttt gttatatttc
    15781 atcaaagcgg tcatttacct gcaataacca gaagaatgag gccggccaga taggcgatga
    15841 acattagcga gataagagtg ttgaccattt tggatgcgcc cctgaattcc ttccagatga
    15901 acacacccca gagggcagca atgagcgttg ctccctgtcc gagaccgtat gaaattgcaa
    15961 accctgcttt tccggatgcc aggatgctca tcgacatgcc aaggttccag atcaccccgc
    16021 ccaggatacc ggtgagatga acccctgcgc tgcctttcct gtaatcactg aatgaaagag
    16081 gctttccttc gaatggcttc ttcataatca aatagttgaa gaggaagtta cttgccagta
    16141 cacccagtgc aaaaaagaaa acagcaccgt atggcgtgag cttgccgggt tcgggattca
    16201 cgaaatcggt tgccatcgaa ctggcaacaa accgatagaa cagagccatg agaacgccgg
    16261 ccgacacgga aagcaggatt cccttgcctg aaaccttctg ttgtgataca ctatgctttt
    16321 tgtaggcaac tgcatctatt actatggcag taacaatgag tgccacaccg gcaaacagga
    16381 taacgggatc tcccttgggt gtaaatatgt aattgattat tactcccagc gccagagcga
    16441 tgccgacgcc caccggaaaa gcaagtgcca tccctgcgat ggccatggct gcaaccagca
    16501 gaatatttga ggcattgaat attattcccc cggctattgc cctgaaaata tttgcagatt
    16561 cagcctggcc gatatcggct gcaaaactcc gtccctgtgt tccggaactg ccgagggtga
    16621 atccaagcag aagcgaaaac agcagtattc caataacata atcccagtag aacaattcaa
    16681 agcgccatga cttccctgag agtttctggg tattgcccca tgagccccag cacagcattg
    16741 tgacaatgca caggaccacc gcaagtgagt aggattgtgg aatgaacata attgaaaaga
    16801 taaaaggata acagaaaaag aataaaaata aaaagataaa agttaacagg gctaagataa
    16861 aagggaaaat aatttaaata ttacaacaac agtaaataaa aatgccggaa aactctttca
    16921 gctttcatga ttaacagggc cacattctgt t
//
LOCUS       DAOOYG010000052        16903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3311_length_16903_cov_6.590871, whole genome shotgun sequence.
ACCESSION   DAOOYG010000052 DAOOYG010000000
VERSION     DAOOYG010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16903
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3311_length_16903_cov_6.590871"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PK288_06215"
     CDS             complement(<1..468)
                     /locus_tag="PK288_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18550.1"
                     /translation="MKKYLVIFFLISALSGCKTWNRSFKAALQTITADDMKKHISVLA
                     SDEFLGRAPSTEGETKTISYLAEQFRQIGLQPAARGSYFQEVPLVKLTADHDMKLVIT
                     GGKNMVRLKYSDDFIGNTPQRKDHIRIDNSDMIFVGYGINSTEYGWNDYEGLDV"
     gene            complement(714..2066)
                     /locus_tag="PK288_06220"
     CDS             complement(714..2066)
                     /locus_tag="PK288_06220"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPM18551.1"
                     /translation="MKTTDYSRREFLAKSSLGGLGLLTAANTSGCRRDFERSSMTVGQ
                     RAKIDTLGGMTLEQLRDQYRAELFDNFIPNMDRYAIDHEYGGVMCSMDIRTGELENMN
                     KTAWYVGRGLWVYSYMYNNLEKNARFLEIAARSKDLLLKIQPADGSFWTESFTREGVP
                     LSGPGDIYGSLFVAEGLSEYAEASGEKQYRIMAKKIIFDCVRRYDQPDYSYSIGSYLK
                     NSPAIQGPRILGHWMILLSLSTQMLMHEPDEDLENLAARCVDAILNHHVNKEYGLTNE
                     VLNHDFSLPDNAYAQHSITGHGLETLAFVMFEAGRRRDGKLFRSAEKVFRKIVDVCTD
                     HVYGGYFHTLQQVDNNIWSTEKSLWCQQEVINGTLFMIEHTGDEWAIGHFERTNPYIF
                     DKYYNPAHKFWHFGGDRKMAYRNVTLMEHYHHARQFIRGLLTFERLIERGGRISGLFA
                     "
     gene            2647..3108
                     /locus_tag="PK288_06225"
     CDS             2647..3108
                     /locus_tag="PK288_06225"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPM18552.1"
                     /translation="MKTERRSVLKKLFAAFIGASGASLTARAGSGEGMEKVTAEAVNF
                     QDQPLFSPFTRYGNLVFVSGHGKRLEGDIKVHTDNVLKMIEGELIKAGSSMEKVLKVT
                     VFLETMDDYNGMNEVYKGRFGKNPPARTTVAVPNGLPPADHLIQMDCIAYI"
     gene            3556..5127
                     /locus_tag="PK288_06230"
     CDS             3556..5127
                     /locus_tag="PK288_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPM18553.1"
                     /translation="MIKKLFFIWVTTVIALNVNSQQSKEIDRIIELGRTDNRTMHHLD
                     IITNRFGGRLVGSDAYENAALWCASEFKKWGMEVILDEAGSVPVGFNRGPWFGRLLAE
                     NGMTLHFATPSYTSGTKGVQRGHVLIEPRNREEFERMKGALKGAWVLIGGKNDGWPVD
                     YSAKADSVRAGIIAYNDSIGKINREIMFWNFANRGKPARELHQLKEEPALFYREMREA
                     GILGIIQSSAVPIRALYDRKNLNKMTFDNLPTVCDIKLDEQQYAIIEKMAGERRYFLL
                     EFDIRNHFKPGPVRFHNVIGIIRGTRYPDEYVMMGGHLDAFDVATGGVDDGSGATPVM
                     EAARLIMAAGGKPKRTILACLWAGEEFGLLGSKHWVESNKDKLPKISNYFNRDGGPTV
                     ASGLEVPEAMYEDFEKVCRPLNRINPDFPFTLTKNTNPPRPRPATAGSSDHAYFAMNG
                     VPTLSFDTPDTKGYDFSYMEIWHTEKDTYDKSIAEYQEHTSVVMAVVVYGLANLDHLL
                     SRKGLYLEPEVKKTK"
     gene            complement(5148..5438)
                     /locus_tag="PK288_06235"
     CDS             complement(5148..5438)
                     /locus_tag="PK288_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPM18554.1"
                     /translation="MRRSKTISLADAMKDYLREMKLEDKLREISLVNSWEEVVGRAIS
                     SRTLKIYIKDGVLYVHLSSSVARNELMMLREELMDKLNQKAGSEVITRIVFR"
     gene            complement(5425..6570)
                     /locus_tag="PK288_06240"
     CDS             complement(5425..6570)
                     /locus_tag="PK288_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPM18555.1"
                     /translation="MYIKRLTLTNFKNYAQADLEFSPRINCFVGNNGVGKTNILDAIH
                     YLSLTKSFFNNIDSVSIRHGDDFFLLQGIFRRGGDEDNIVCSFHRQKQKVLRRNSREY
                     QRLSDHVGRYPVVMISPADSSLINEGSEERRKFMNKIISQYNPGYLDSVLNYNKALQQ
                     RNKLLRDFAGRSDFDTEMLDVWDVQLMKYGEHIFGERKLLMEELGPVFQEYYSMISGN
                     KERVRLIYNSHLIKGDFGETLKLALKKDLLLEYTTTGIHKDELVFEMNGYPVRTLGSQ
                     GQQKSFLTALKLAKFDYIKRKAGFAPILLLDDIFDKFDAGRVTSIIKLVGNHRFGQIF
                     ITDTHQDRLEVILNETGIEYRLFRIGESGIGEILQNGENGNMTDEAE"
     gene            7078..7767
                     /locus_tag="PK288_06245"
     CDS             7078..7767
                     /locus_tag="PK288_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM18556.1"
                     /translation="MAKGKKKGENPQGLKNVEETLTKTEQFLESNYKALLAGLGVIVL
                     LVGLIWLGKLYLNNRNDEAQVQMYQAQRYLESDSLNLALNGDGNYLGFIDIANDYKYT
                     KAGNLARYCAGICFLHLGHYEEAIDYLGKYSKKDKVIGSLAIGAIGDAYVELGDTKKG
                     LSKYLEAAEYADNSFNTPVFLMKAAELYEMENNYSKALQIYQRIKDEYPESTEGTTID
                     KYIARVKILSE"
     gene            7767..8252
                     /gene="ribH"
                     /locus_tag="PK288_06250"
     CDS             7767..8252
                     /gene="ribH"
                     /locus_tag="PK288_06250"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPM18557.1"
                     /translation="MATANLSEYAPGSVPDAGKMRFGIVVSDWNHQITGSLLKGAVET
                     LKKHGASESNIIVKHVPGSFELTLGAQFLAEYEDLDAIICLGCVIQGETPHFTYICQG
                     VTHGITQLNLDYNIPFIFGVLTTLNLEQAEERSGGKHGNKGDEAAVTAIRMAALQREM
                     E"
     gene            complement(8271..11171)
                     /locus_tag="PK288_06255"
     CDS             complement(8271..11171)
                     /locus_tag="PK288_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPM18558.1"
                     /translation="MEYFLERIARILTDGFVDRLDTQCIVFPSRRAGLFFMKYLADRV
                     DKPVWAPSVMTINDLFRLFSPLRLADNEVLLFELYKAFRSVRKTTETFDDFYYWGGML
                     LNDFDDIDKYLADPASIFRNVRDLKMIDRQFGGLSQHQAEVVQKFWVNFEPGKPTPEK
                     KQFEETWYILNDLYNAFREALRQKRLAYEGMIFRDAIERKGWQNPKALRWDKIHFAGF
                     NALNECEKTLMSSLKNNGKARFYWDYDNSYLGSKDVNSAGYFLRENILSFGNDMPADW
                     SYDTLLTDKSGTVRRTVIETSSDVAQVKLVSRLTAETDDPEQGSDHQTAIVLADENLL
                     IPLLSSLPEKRDHVNVTMGYPMRETPVYQLAKSLLDLQSNAREKDGRVSFHHSDVMKV
                     LENSLVSPLIGKEGKLLAREVANGNSEYIPSDYFGSSGFTRIIFEKQDDPPMLAEYLR
                     KIFFFIAREDTEEMSDQPGTFMTKKIRNEFIYRAVLSINRMKTLTGDPEVNFTVRTFA
                     AMLERILRAQSVPFIGEPLKGIQIMGLLETRTLDFRNIIILSVNEGVLPSLTTASSFI
                     PFSLREAFGLPSINHQESVYAYHFYRLLHRARNVTFIYNSGSEGLRSGEISRFILQMK
                     YGSVVAQPDHLSLNLEIKNPASPGEFLERTETMNLKLMSRFSGNAGAKIISPSAINVW
                     LGCRMKFWYRYVNDIREPVKQKGEIDPATLGSIFHDVAKRLYMPYLGQTIEASGFEAL
                     GKNREYLGILIDTAVREHFPDNSDSYSALNELISENVLMTYISRILETDRKYAPVRII
                     ALEKPVQFRIGSPAEKREAGFLVGGIIDRIDSTGGTTRIVDYKTGSVADHIKAIGELF
                     EDDRKRDIDCWLQALLYCEGYISGSSDPVVAPAIYKIRRIPGDRPAEMLSIKVPGTDE
                     VVVGDYNMIRDEYLDGLQKTIKAMFDIDEPFYMTRDWVGKCSYCEYRRLCKR"
     gene            complement(11199..12173)
                     /locus_tag="PK288_06260"
     CDS             complement(11199..12173)
                     /locus_tag="PK288_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133901.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPM18559.1"
                     /translation="MREKNKESRIYLWGHERRFNAYSNYFRQLYGTRVQKVSVDAGFT
                     CPNRDGKKGTGGCTYCDNQAFNPSYCDPVKSITQQIKEGIEFHKWRYRRAVSYLTYFQ
                     SYTNTYAPLEHLRKLFMEALDYPGVIGLTIGTRPDCIGKEKLEYLGELSKDHYITVEY
                     GIESCYNRTLERINRGHSFEEAVEILKQTAEYGIPAGAHFIFGLPGETVEDMLSMAEI
                     ISSLPLRTVKFHQLQILKNTRMEKEYRNKIQDFHIFTLEEYINFIVKFLEMLNPAIVV
                     ERFTGEVPPRFRVNEGFGNYRSEQILILVERRLEELDTWQGRLCKTMN"
     gene            complement(12173..15430)
                     /locus_tag="PK288_06265"
     CDS             complement(12173..15430)
                     /locus_tag="PK288_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPM18560.1"
                     /translation="MGEGTLTIYSASAGSGKTFRLASSYLAALFRSKYQYRRILAVTF
                     TNKATAEMKGRILDQLDRLASGRDSEYLGDLVAATGKSEQDLRAAAKEILSSILHDYS
                     RFSVSTIDAFFQKVIRAFARECGLNSAFSLEIDHSLILTTAVDEMIASTAKDRNLRNW
                     LMRYILSNIDEEKSWNIKDSIIRLSEELFREKFRILSSGEIGNLKDKNFLLRYIEKLS
                     TLRSSFEKRMISFGKKAEQIFSEFGLTDDMFYNKSRGVPGFIRDLSKGRIVRPGEKVR
                     AALGNPPRWATGNPHPHLLSAVTGGLESVLKEAVRLFDETITDYNTAGSILQNFYALG
                     ILSDVLAKVHEVVSGENMFLLSDAGELLSLITGNDQTPFIYEKIGSRYENFMIDEFQD
                     TSVLQWNNFRHLIENSMSEGNDNMVVGDVKQSIYRFRNSDWKILGDMLEKKTDNNRIK
                     SIPLGRNYRSRSNIISFNNSFFSVLPVLLDRVFSESPGDTSFVKLYAEAVQSDPGRST
                     GGYVRIEFIGDDAEGEQMTDTEGRINKDTGKKQGNDWKAKVLKKIPGIIETILDKGYN
                     ASDVGILVRDGREGAEVMKTLLGYSGSLQESPGQHRFKVVSNDSLVFSGSNVITFIIS
                     ALKVIVNPEDQISRALMTRYYLFAKEVPGADRINLFREDLISGSAGYLPDGYELFLEE
                     AGKMTLYDAAEAIIDFFGVGDYSWNVSYLVSFMDIVLEYSRTRNTDFRSFIEWWESGG
                     ESKSLSLPSGMDAVRVLTIHKSKGLEFPVVILPFLSWNLDHRPSRRPVIWARPGRPPF
                     NDIGIVPLKYSKDLSETIFNVEYHEERFNSFLDNLNLLYVAMTRAIDALYGFVPLNPV
                     SSGGIASYIREAMQCDLNPAGLSGIVMSKMSDNEGRVFELGEVPLNQEHGREAGRSIT
                     LTSYHSGKRPQSLRLKLHAGNYFVTDGERLREMTNYGLIMHEVFAGISCREDIPEAVA
                     GMVLEGKITENEAGALVERLQDLVSDPAVSSWFDPGNTLMKEAEILLPSGSIKRPDRI
                     ILRNGNATIVDFKFGEKDPKHAEQISLYRNLLVMMGYRNTDAYIWYVDANLIEKA"
     gene            15479..16678
                     /gene="mnmA"
                     /locus_tag="PK288_06270"
     CDS             15479..16678
                     /gene="mnmA"
                     /locus_tag="PK288_06270"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPM18561.1"
                     /translation="MKKKVVIGLSGGVDSSVAAWVLKNEGYEVEALFMRNWNDTTGLL
                     KSDCHWEDDLMFAEMVAKKLKIPFRFIDLSESYRKRVADYMFSEYEKGRTPNPDVLCN
                     REIKFDSFAREALKTGADYMATGHYCRKDAIKRDGKIIFRLLSGMDNNKDQSYFLCQV
                     SQKQLAKALFPIGEMTKPRVREIADELKLPTARRKDSQGICFIGKVHLPTFLKQKLKS
                     EKGEVIRIPAEKCITRWLRGIPGPELDDSLLLMNTTPPVYSPETGSVAGVHEGAHFFT
                     IGQRKGLNIGGSKEPLFVIGTDTLNNIVFVGEGEAHPGLYRKGLRIRRDDVHWIRKDL
                     ELNENESRLYMVRIRYRQPLQKARLYRRNNDIHIIFDRLQRGITAGQFAAWYDGDELI
                     GSGAIDG"
     gene            complement(16695..>16903)
                     /locus_tag="PK288_06275"
     CDS             complement(16695..>16903)
                     /locus_tag="PK288_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HPM18562.1"
                     /translation="GDVMGDWSFEKPLRPGDRVIFLDMIHYTMVKTTTFNGVQHPSIG
                     IWTADNSFRLLRQFGYEDYKGRLS"
BASE COUNT         4542 a         4064 c         3665 g         4632 t
ORIGIN      
        1 cacatcaagg ccttcataat cgttccatcc atactccgtg gaattaatcc cgtatccgac
       61 aaaaatcatg tcagaattgt caatcctgat atgatccttc ctctgcgggg tgttcccgat
      121 aaaatcgtcg ctgtatttca gtctgaccat attcttcccc cctgttatga cgagtttcat
      181 gtcatggtca gccgttagct tgaccagggg aacttcctgg aagtagctgc ccctggcagc
      241 aggctgaagt ccgatctgcc tgaattgttc ggccaggtaa ctgatagtct ttgtttcgcc
      301 ctcggtggac ggggccctgc caaggaattc atccgaggca agtaccgaaa tgtgtttctt
      361 catgtcatcg gcagtgatgg tctgcaaggc ggccttaaag ctcctgttcc aggtcttgca
      421 gcctgataag gctgagatca gaaaaaagat aacaaggtat ttcttcatct tttcagcagt
      481 gattaaattc atccagtaaa tcaaaattat ggttcagggc ttaaaaataa ctgatttatc
      541 ggatgatcat gcatcatacc gaattatgga tgcagacgat tgtcattttt taggttgatc
      601 aggaaggaac ccttgtttca atcctctgtc atacctatcc ttccggaatc attaagcaca
      661 cgaaaaaaca tggccttcca ggctgctgac acagacccac caaagggtaa ttctcaggca
      721 aacaaacctg atatcctgcc tcccctttca atgagacgtt caaatgtaag cagaccgcgg
      781 ataaactgcc gggcatgatg gtagtgttcc ataagggtca cattgcgata tgccatcttc
      841 cggtcaccgc cgaaatgcca gaatttatgg gccggattat aatatttatc aaaaatatat
      901 ggatttgtcc tttcaaaatg tccgattgcc cattcatcac cggtgtgttc aatcataaac
      961 agagtaccgt tgatgacttc ctgctggcac cagagtgatt tttccgtgct ccagatgttg
     1021 ttatccacct gctgcaaggt atggaaatat ccgccataga cgtgatcggt gcagacatct
     1081 acaattttcc tgaaaacttt ttccgcagac cgaaacagtt tcccgtccct gcgacggcct
     1141 gcctcgaaca tgacaaaggc aagggtttcc agaccatgcc cggtaattga atgttgtgca
     1201 tatgcgttat ccggcaggct gaaatcatgg ttcagaacct cgttcgtaag tccatattcc
     1261 ttgttgacat gatgattcag gattgcatcg acgcatcgtg cagcaagatt ttcaagatct
     1321 tcatcaggct catgcatgag catctgtgta gacaggctca gaaggatcat ccaatgaccc
     1381 aatattctcg gtccctgaat tgcaggtgaa tttttcaggt aactgcctat actgtaggaa
     1441 tagtcaggct gatcgtaacg cctgacacaa tcaaaaatga tcttctttgc catgatcctg
     1501 tattgtttct cccccgatgc ctcagcatat tccgacaggc cttccgcaac aaacaaactg
     1561 ccatatatgt cccccggccc ggaaagaggc acaccttcac gggtaaaact ttcggtccag
     1621 aaactgccgt cagcgggttg aattttcagg agcagatcct ttgatctggc tgcaatctcc
     1681 aggaagcggg cattcttttc aagattattg tacatgtatg aatataccca caatcccctg
     1741 ccaacgtacc atgcggtttt attcatgttt tccagttcgc ccgtgcggat atccatcgaa
     1801 cacattactc ctccgtattc atgatcaatc gcgtaccggt ccatgttggg aataaaattg
     1861 tcaaacagtt cggccctgta ctgatcccgc agctgttcga gagtcattcc tcccagagtg
     1921 tctatctttg cccgttggcc tacggtcatg gaggatcttt caaagtcgcg cctgcagccg
     1981 gatgtgtttg cagcggtcag caagccgagc ccgcctaatg agcttttggc taaaaattca
     2041 cgcctcgaat aatcagtcgt tttcatacct gatatttata actttccaca agaaataact
     2101 ttatgccgga tttgctgaaa tgatgcaaac aagtgcaacc atatgcaacc tgacatgaca
     2161 ctcagaataa cctgtcccca atacagcata aacgtgtcat tctgatattg cctggaaatt
     2221 tactcattat tactgacatc atgacacatt aattccaaca gccctgtcgt aatcacacgg
     2281 tctgaaaggt tcttttcccc ggcctgaata acttgctgaa ttcagatcaa taatattcgg
     2341 cttatcttta agtgcatgag gattaaaatt ctgacggtaa catcatcact ccaatgtgat
     2401 ccggagaata acaacgtatg tatgggaaaa taatttaaat aataatcccg gttatatgcc
     2461 tttaaggcgg tttaacatgg taaatttctg taagttgcct gaagatgcga aattcccggt
     2521 gagaatctta taaaccaaac ttcgtaggag tgagttgttt gcagaaatgg ttttattccc
     2581 atattttagc tttgcgggag caatcataat ttgacctgac acagtgattt caaataaacc
     2641 tacagtatga aaacagaaag aagatcagta ctcaaaaaat tattcgctgc atttataggt
     2701 gcatccggag caagcctgac agcaagggca ggatcagggg aaggcatgga aaaggtaact
     2761 gcggaagcgg taaatttcca ggatcagcct ttgttttcac cttttaccag gtacggtaat
     2821 cttgtatttg tttcaggtca tggaaaacgg cttgaaggtg acataaaggt acacaccgac
     2881 aatgtcctca aaatgattga aggcgaactc atcaaggcgg gctcttcgat ggagaaggtg
     2941 cttaaggtaa ccgtattcct ggaaaccatg gatgactata acgggatgaa tgaagtatac
     3001 aagggacggt tcggaaagaa tcccccggcc aggacaaccg tagcagtccc caacggtctg
     3061 cctcccgcag accacctgat ccagatggat tgcattgcct atatctgaaa acaataattc
     3121 atcagctcag gttattggca gctattcctc agcattgtca ccattgtgat tgatccggga
     3181 cccccctgta aatcttcgtt ttattccatt tgtagacatg tgattgccgg gtatccgcag
     3241 tttttgtcgt acataaacga aattgaccgg agctttcagt cccaatattc atttcccttg
     3301 ccgcacagag ggaatgcaca ggaggaataa gtcgtaacag gctgatttaa tatggtttat
     3361 tattgtcatt taaacagctc aatatgatct tttacaaaga aacattgaaa caattgttta
     3421 attcccaacc ccggatgtca atagggcaaa agcgttgata tttttgcttc tattttatct
     3481 tttgataaac ggaggtgata tattttttaa atataattag tagaattgtg gcttgcattt
     3541 aaaaaacacg gcagaatgat taagaaactt tttttcatat gggtcaccac agtgattgcc
     3601 ttgaatgtaa attcacagca atcaaaggaa atcgacagga tcattgaact gggcagaaca
     3661 gataaccgga ccatgcatca ccttgatatt ataacaaacc ggttcggagg caggctagtc
     3721 ggctccgatg cctatgaaaa cgcagccctt tggtgtgctt cggaatttaa gaagtggggt
     3781 atggaagtga ttctggatga agctggaagt gttcctgtag gctttaatcg ggggccctgg
     3841 ttcggaaggc tgcttgcgga gaacggcatg acacttcatt ttgccacgcc atcatatacc
     3901 tccggaacaa aaggagttca gcgggggcat gtacttatcg agcccaggaa ccgtgaagaa
     3961 tttgaaagga tgaagggcgc cctcaaaggg gcctgggtac tgataggcgg caaaaacgac
     4021 ggctggcccg tggactattc cgcaaaagca gattctgtcc gggcagggat aatagcatac
     4081 aatgactcca tcggaaagat taaccgtgaa atcatgttct ggaattttgc gaatcgcgga
     4141 aagccagcca gggaactaca tcagcttaag gaagaacccg ctcttttcta ccgcgagatg
     4201 cgcgaagcag ggatactcgg cataatccag tcgtcggctg ttccgataag ggctctctac
     4261 gacaggaaaa acctgaataa aatgaccttt gacaacctgc cgacagtttg tgacataaag
     4321 cttgatgagc agcaatatgc catcatcgaa aaaatggccg gggaacgacg ctacttcctc
     4381 cttgagttcg acatccgcaa tcattttaaa cctggcccgg tcaggttcca taatgtgatc
     4441 gggatcatca ggggcacgcg ttatcccgat gaatatgtta tgatgggagg ccacctggat
     4501 gcttttgacg ttgcaacggg cggagttgat gacgggtcgg gtgcaactcc ggtcatggag
     4561 gccgccaggc tgataatggc agccgggggc aaacccaaaa gaactatact tgcatgcctg
     4621 tgggctggag aggagttcgg gcttctcggg tcaaaacact gggtggagtc aaacaaggac
     4681 aaacttccga aaatatcaaa ctatttcaac cgcgacggcg gtccgacagt ggcatcaggc
     4741 ctggaagtcc ccgaagccat gtatgaggat ttcgaaaaag tgtgcaggcc attgaacagg
     4801 atcaatcctg atttcccttt caccctcacc aaaaacacca atcctccccg gccgagaccg
     4861 gcaactgccg gcagctccga tcatgcctat tttgccatga acggggtccc gacactgtcg
     4921 ttcgacaccc ccgataccaa gggttacgat ttcagctaca tggaaatatg gcacactgaa
     4981 aaagatactt atgacaagag tattgcagaa taccaggaac acacttcggt ggttatggcg
     5041 gtagtagtat acgggcttgc aaatctcgac cacctgcttt caagaaaggg actttacctg
     5101 gaaccggaag tcaaaaaaac aaagtgaagg ataattatcg tttaccctca cctgaataca
     5161 atccgggtga taacttctga tccggctttc tgattcagct tgtccatcag ttcctccctc
     5221 agcatcataa gttcatttct ggcaaccgac gagctgagat gtacatacaa aacgccatcc
     5281 ttaatgtata tttttaatgt tcttgacgat atggcccttc caactacctc ctcccaggaa
     5341 ttgacaagac tgatctcacg cagcttgtcc tccagtttca tttcacgcag ataatccttc
     5401 atggcatcgg ccagcgatat cgttttactc cgcctcatca gtcatatttc cgttttcacc
     5461 attctgcagt atctctccta ttcccgattc gccgatcctg aaaagcctgt attctatccc
     5521 ggtctcattc agaataacct cgagcctgtc ctgatgtgtg tccgtgataa agatctgtcc
     5581 gaatctgtga ttgccgacca gttttattat actggtcacc cggccggcat cgaatttgtc
     5641 gaaaatatca tcaagcaaaa ggatcggggc aaatcctgct tttcttttaa tataatcaaa
     5701 ctttgccagt ttaagggccg tcaggaatga tttctgctga ccctgtgagc caagggtgcg
     5761 aacgggataa ccattcattt caaatacaag ctcatcctta tggatccccg ttgtcgtata
     5821 ttcaagcaac aaatccttct tcagcgcaag cttcagggtc tccccaaaat cacccttaat
     5881 gaggtgcgaa ttgtaaatca gccgtactct ttccttattt cctgagatca tggaataata
     5941 ctcctggaaa acagggccaa gttcttccat aagaagcttt ctctctccaa aaatatgctc
     6001 cccgtatttc atcagctgta cgtcccacac atcaagcatc tcggtatcga aatctgatct
     6061 tcccgcgaaa tccctgagaa gtttgttcct ctgctgaagt gccttgttat agttgagaac
     6121 ggagtcaagg taacccggat tgtactggct tattatcttg ttcatgaact tcctccgttc
     6181 ctcactccct tcattgatca gtgaactatc agcgggtgag atcattacga ccggatatcg
     6241 gcctacatga tcagataacc gctgatattc cctgctgttc cgccttaaaa ccttttgttt
     6301 ctgcctgtga aaggaacaga cgatattgtc ctcatccccg ccccttctga aaattccctg
     6361 aagaagaaag aaatcgtcac catgcctgat gcttacgctg tcgatattgt tgaaaaaact
     6421 ttttgtaagg gaaagataat gtatggcatc gaggatattc gtcttgccga caccattgtt
     6481 gccgacaaaa caattgatcc tcggggagaa ctcgagatcc gcctgggcat aattcttgaa
     6541 attggtaagg gtcagccttt taatatacat aaataccgtt ccttcctttg aattaaaata
     6601 ttcagagcca caatcatgtt tcgtgaggca ataagccgat tgttttattt gcttccctga
     6661 ttcaaaaaca taactgtatc agattgcaaa tataacctaa agatggcctg gccggtacat
     6721 ccggctgacc attttagccg cggcgacgga aaatataaat atgcgcaaat cagcaggaat
     6781 attttcggaa cagtggaatc aggcctaatg ctgcctccag tgattggtca gccaattatc
     6841 agtggctgcc tgcgtgtact tttccagatt gattttatgt attccgggtt cattatctat
     6901 tccggcatga aacctgctga aatcatttct cgggcagatc cgattcatat atttgatatt
     6961 caatatatta ttatcgaatc cctatctcat tgaattttta attaattttt tcataataat
     7021 tttaaaagaa ctacttttgc ggagttaaaa ttttaagtgt tttaaggata aactgttatg
     7081 gcaaaaggca agaaaaaagg cgaaaatccg cagggcctga agaatgttga ggagacattg
     7141 accaagaccg aacaatttct tgaaagtaat tacaaggcat tgctggcagg tctgggtgtg
     7201 attgtcctgc tggtgggatt gatctggctt ggaaaactct atctcaacaa ccggaatgac
     7261 gaggcacagg tacaaatgta ccaggcccag agatatctgg aatcggattc tttgaatctt
     7321 gcactgaacg gagatggcaa ctatcttgga ttcatcgata ttgccaatga ttacaagtac
     7381 accaaggcag ggaatcttgc acggtactgt gccgggatct gttttcttca tctcgggcat
     7441 tatgaggaag caatcgatta cctgggaaaa tattcaaaga aggataaagt tatcgggtcg
     7501 cttgccattg gagccatcgg cgatgcctat gttgagctgg gcgatacaaa aaaaggcctg
     7561 tcaaagtatc ttgaggctgc tgaatatgct gataattcct tcaatacacc cgtttttctg
     7621 atgaaagctg ccgaacttta cgaaatggag aacaattatt ccaaagcatt gcagatatat
     7681 cagaggatca aggatgaata tcctgaaagt accgaaggca caacaatcga caaatacatt
     7741 gcaagggtaa aaatactttc tgaataatgg ccacggcaaa cctttccgaa tatgctcccg
     7801 gatcggtgcc cgatgccgga aaaatgaggt ttggcattgt tgtatcggac tggaaccatc
     7861 agattaccgg ttcgttgctg aagggagcag tggaaacatt aaaaaagcac ggggcttccg
     7921 aaagcaatat aatcgtaaag catgtacccg gaagctttga gcttacgctg ggagctcaat
     7981 tccttgccga atatgaggat ctggatgcaa tcatctgcct cggatgtgtc atacagggtg
     8041 aaacacctca tttcacatac atctgccagg gtgtaaccca cgggatcaca cagttgaatc
     8101 ttgattacaa catacctttt atttttggcg tcctgacaac cctgaacctg gaacaggcag
     8161 aagaaaggtc ggggggaaag catggcaaca agggcgatga agctgcagta accgcaatac
     8221 ggatggctgc acttcagcgt gaaatggagt gatatccgcc ccggttacag ttatctttta
     8281 catagcctcc tgtattcaca gtaggaacat ttccccaccc aatcacgggt catgtaaaat
     8341 ggttcgtcaa tatcgaacat ggcctttatt gttttttgca gtccatcaag atattcatcc
     8401 cttatcatat tataatcccc gactacaacc tcatctgttc ccggcacttt tatgctcagc
     8461 atctcagccg gcctgtcccc gggaattctt ctgatcttgt agattgcagg tgcgacgacg
     8521 ggatcggaac ttcctgaaat gtacccttcg cagtacaaca atgcctgcag ccagcagtca
     8581 atgtcccttt tcctgtcatc ctcgaaaagc tcccctatag cttttatatg atcagccacc
     8641 gaaccggtct tgtaatcgac tattcttgtt gttcccccgg tactgtctat cctgtcaata
     8701 ataccgccta ccaggaaccc ggcttctctt ttttctgctg gtgatccaat cctgaactga
     8761 acaggttttt ccagggcaat gatcctgaca ggagcatatt tcctgtcagt ttccaggatt
     8821 cttgaaatgt aggtcataag tacattttca gagatcagct cattaagcgc tgaataggaa
     8881 tcgctgttat ccggaaagtg ctccctgaca gcggtatcta tcaatatgcc cagatattcc
     8941 ctgttcttcc caagagcttc aaaacccgaa gcttcgattg tttgtccaag gtagggcata
     9001 taaagtcttt tggcaacatc atgaaaaata cttcccaatg ttgccggatc tatttctccc
     9061 ttctgcttca ccggttccct tatatcattc acataacggt accagaactt catcctgcat
     9121 ccgagccaga cgtttattgc cgagggggaa atgatcttcg cgccggcgtt accgctgaac
     9181 ctcgacatta gtttcaggtt cattgtctcc gttctttcca ggaattcccc gggggatgca
     9241 ggattcttta tttcaagatt caggctcaga tggtcaggct gtgctacaac agatccgtac
     9301 ttcatctgca gaataaacct gcttatttct ccgctcctca aaccttctga gccggaattg
     9361 taaataaatg tcacattccg ggccctgtga agaagcctgt aaaaatggta tgcataaacc
     9421 gattcctggt gattgatcga gggcaggccg aatgcctcgc gcaagctgaa cggaatgaat
     9481 gaggatgcag ttgtaagaga tggcagaact ccttcattaa ctgaaagaat tataatgttc
     9541 cggaaatcaa gggttcgggt ctcaagcagt cccattatct ggatcccttt aagcggttca
     9601 ccgatgaacg gtaccgactg ggctcggaga atcctttcaa gcatggccgc aaaggttctc
     9661 accgtaaaat tcacttcagg atcacccgtg agcgtcttca tccggttaat cgaaagcact
     9721 gccctgtaaa taaattcatt ccttattttt tttgtcatga atgttccggg ctggtccgac
     9781 atttcctcag tatcttccct ggcgataaag aaaaatatct ttctgagata ttccgccagc
     9841 atcggaggat catcctgttt ttcaaatata atcctggtaa atcccgatga accaaaataa
     9901 tcagaaggta tatattcaga attgccgttc gcaacctccc tggccagtaa cttcccctcc
     9961 tttccgatca gcggtgacac aagactgttt tccaacacct tcattacgtc tgaatgatga
    10021 aagctgacac ggccgtcttt ctccctggca ttggactgta aatcgagaag gctctttgca
    10081 agctgataaa ccggcgtctc cctcataggg taccccatgg ttacattcac atgatccctc
    10141 ttttcaggaa ggctcgataa cagtggaatc agcagatttt catcagcaag gacaattgca
    10201 gtctgatgat cagagccctg ttccggatca tccgtttcgg cagtgagacg ggaaaccagt
    10261 ttaacctgtg caacgtcgga tgatgtttca atcacggtcc gtcttacagt acccgattta
    10321 tccgtgagaa gcgtatcata gctccagtcg gccggcatat cgttgccaaa ggacaggatg
    10381 ttttctctga gaaaatatcc cgctgaattc acatccttcg aaccgaggta tgaattatca
    10441 taatcccagt agaacctggc ttttccgtta tttttcagac tgctcatcag tgtcttttca
    10501 cactcattca gcgcattgaa tcctgcaaaa tgaattttgt cccaccggag tgcctttgga
    10561 ttctgccacc ctttcctttc aatggcatcc ctgaatatca taccctcata tgcaagcctt
    10621 ttctgtctga gagcctccct gaatgcattg taaagatcgt tcagtatata ccaggtttct
    10681 tcaaactgtt ttttctcggg tgttggttta ccgggttcaa agttcaccca gaatttttgc
    10741 actacctcag cctggtgctg actaaggccg ccgaactgac gatcgatcat tttaagatca
    10801 cggacatttc gaaatattga agccggatct gccaggtatt tgtcaatatc gtcaaaatcg
    10861 ttcagcagca tgcctcccca gtaataaaaa tcatcaaaag tttctgtcgt ttttctgact
    10921 gaccggaatg ccttgtaaag ctcaaaaaga aggacttcat tatccgcaag cctgagtggg
    10981 gagaataaac ggaatagatc atttattgtc attaccgacg gggcccagac gggtttatca
    11041 accctgtcgg caagatactt catgaaaaac aaacctgccc tcctgctggg gaacacaatg
    11101 cactgtgtat cgagccggtc aacgaaaccg tcggtcaata tccttgcaat gcgttccaga
    11161 aagtattcca tctattattc aggataaaac atcattgatc agttcatcgt tttgcagagt
    11221 cgcccctgcc aggtgtcaag ttcttcaagc cgtctttcca cgaggataag gatctgttca
    11281 gaccggtaat tgccaaagcc ttcattaacc ctgaaacgcg gcggaacttc tccagtgaag
    11341 cgttccacta ctattgccgg attcagcatc tccaggaatt tcacaatgaa gtttatatac
    11401 tcctccaggg tgaaaatatg aaaatcctgt atcttgttcc tgtattcctt ctccatccgg
    11461 gtgttcttca gtatctgcag ctgatggaac ttcactgttc tgaggggcag ggaggaaatt
    11521 atctcagcca tggacagcat atcttcaaca gtctccccgg gcagtccaaa tatgaaatgg
    11581 gctcctgcag ggatgccata ttccgcggtc tgcttaagga tttcgacagc ttcttcaaaa
    11641 ctatggcccc tgttgatcct ttccagggtc ctgttatagc atgattctat tccatattca
    11701 actgtaatat agtgatcttt tgacagttcc cccaggtatt ccagcttttc ctttccgatg
    11761 caatcgggcc tggtcccgat tgtcagaccg atcacacccg gatagtccag ggcttccatg
    11821 aacagcttcc tgagatgttc cagcggggca tatgtattcg tgtatgactg gaaatatgtc
    11881 agatatgata ctgccctgcg atacctccat ttgtggaatt ctatgccttc ctttatctgt
    11941 tgtgttattg atttcacagg atcacaataa gatggattaa atgcctgatt atcgcagtac
    12001 gtgcatccgc ccgtaccttt tttcccgtcc ctgttcggac aggtgaatcc cgcatcaacc
    12061 gatactttct gtaccctggt gccatacagc tgcctgaaat agtttgagta tgcattgaac
    12121 cgccgttcat gcccccagag atatatcctg ctttctttgt ttttctcccg catcaggctt
    12181 tttctatgag atttgcatca acataccaga tgtaggcatc agtattccga tagcccatca
    12241 ttacaagtaa attacgatac aggctgattt gctctgcatg cttaggatcc ttttcaccaa
    12301 acttaaaatc aacaatcgta gcattcccgt ttctcaggat aattctgtcg ggtcttttta
    12361 tactgcctga aggaagcagt atttccgcct ctttcataag agtattcccg ggatcgaacc
    12421 aggatgatac tgccggatcc gatacgagat cctgtaatcg ctcaacaagc gccccggctt
    12481 cattttccgt aatttttccc tcaagcacca taccggcaac cgcctccgga atatcttccc
    12541 tgcatgatat ccctgcaaaa acctcatgca tgatcaatcc atagttcgtc atttccctga
    12601 gccgttcgcc atccgttaca aaataattcc cggcatgaag ttttagtctc agggattgcg
    12661 gcctttttcc cgaatggtag gaagtcaggg ttattgatct gccggcttcc ctgccatgtt
    12721 cctgattcag tggtacttca cccagttcaa ataccctccc ctcgttgtcg gacattttgc
    12781 tcataacaat acccgacaac cctgccgggt tcaaatcaca ttgcatcgct tccctgatat
    12841 atgaggcaat tccgccggat gacaccggat taagcggaac aaatccgtat aatgcatcaa
    12901 tagccctggt cattgcaaca tagagcaggt taaggttatc aaggaatgaa ttaaaacgtt
    12961 cctcatggta ttcaacgtta aaaatcgttt ccgataaatc cttgctgtat ttcagtggaa
    13021 cgattccgat gtcattgaaa ggaggccggc cgggccgggc ccagatcaca gggcgccttg
    13081 aaggacggtg atcgaggttc catgaaagaa aaggaagaat tacaaccggg aattccagtc
    13141 ctttcgattt atgtatggtc agcaccctga ctgcatccat acctgacgga agagacagtg
    13201 actttgactc gccgccggat tcccaccatt ctatgaacga cctgaaatcg gtattccttg
    13261 tcctcgaata ctcgagtaca atatccatga aggaaacaag gtaggatacg ttccatgaat
    13321 agtccccgac accaaaaaag tcaattattg cctcagcagc gtcgtaaaga gtcatctttc
    13381 ccgcttcttc aagaaacagt tcataaccat ccggcaaata ccctgctgat ccgcttatga
    13441 gatcttccct gaacaggttg atccgatcag caccgggtac ttcctttgca aaaaggtaat
    13501 atcttgtcat cagggccctg cttatctggt cctcgggatt gactatcacc tttagagcag
    13561 aaataatgaa tgttatcacg ttcgatcctg aaaagaccag ggaatcgttt gaaacaacct
    13621 tgaaccgatg ctgccccggg ctttcctgca ggctgccgga gtatccgagc agtgtcttca
    13681 ttacttccgc accctcacgg ccatccctta caagtatccc gacatcggaa gcgttataac
    13741 ctttatcaag gattgtttca ataatacccg gaattttctt aagtactttt gctttccagt
    13801 cattgccctg ttttttccct gtatccttgt taatacgacc ctctgtatcg gtcatctgtt
    13861 ctccttccgc atcgtcccct atgaactcaa tcctgacgta accacctgtt gaacggcccg
    13921 ggtctgactg gacagcttcc gcatacaact ttacaaagct tgtgtcaccg ggactttcgg
    13981 aaaaaacccg gtcgagcaac acgggcaata ctgaaaaaaa ggaattattg aaagatatga
    14041 tgttcgatct gctcctgtaa ttcctcccaa gtggtatgct tttaattcta ttgttatctg
    14101 tcttcttttc aagcatatct cccaggatct tccaatcgct gttcctgaac ctgtatatgg
    14161 attgtttgac gtcacccaca accatattgt cgttgccttc cgacatgctg ttttcaataa
    14221 ggtgccggaa attgttccac tgaagaactg aagtgtcctg gaactcatca atcatgaagt
    14281 tctcataacg tgatcctatc ttttcataaa tgaacggggt ctgatcattc ccggttatga
    14341 gacttagaag ttccccggca tcagacagca ggaacatgtt ctcgcctgaa acaacttcat
    14401 ggaccttggc gagaacgtcg gaaagtatac ccagtgcata aaaattctga aggatggaac
    14461 cagcagtgtt gtaatctgta atcgtttcat caaagagtct gactgcttct ttaagaacac
    14521 tttcaaggcc tccggtcaca gcacttagca gatggggatg cggattaccc gtggcccatc
    14581 gcggcggatt tccgagggca gcccttacct tctcgccagg tctgacaatc ctgccttttg
    14641 acagatccct gataaatccc ggtactcccc tgctcttatt atagaacata tcatcagtta
    14701 gtccaaattc tgaaaagatc tgttccgcct ttttcccgaa ggagatcatt cttttttcaa
    14761 aggatgacct caatgtactt agtttttcga tatacctgag caggaaattc ttatccttca
    14821 gattgccgat ctcacccgag gagagtatcc tgaacttttc cctgaacagt tcttccgaaa
    14881 gcctaataat cgaatcctta atgttccagc ttttctcttc atcaatattt gaaaggatgt
    14941 atctcatgag ccagttcctg agattccggt ctttcgcggt tgaagctatc atttcatcaa
    15001 ctgcggttgt cagaatgagg ctgtgatcaa tttcaaggct gaatgccgaa ttaagaccgc
    15061 actccctggc aaatgctcgg ataactttct ggaaaaaggc gtctattgta gaaaccgaga
    15121 aacgcgaata atcgtgaaga attgacgaaa ggatctcctt tgcagccgcc ctgaggtctt
    15181 gctctgattt gcccgtggct gcaacaagat ccccgagata ttccgaatcc ctgcccgatg
    15241 caagcctgtc aagctgatcg aggattcttc ccttcatttc agctgtggcc ttattggtaa
    15301 aggtcacagc cagtatcctg cggtattggt attttgatct gaaaagggca gcaagataac
    15361 tggacgcaag ccggaatgtc tttcccgaac cggcggatgc actgtaaatt gttaatgtcc
    15421 cttctcccat aaccggataa aggtatcaat tttaaagcct tttaactacc tttgcctggt
    15481 gaaaaaaaag gttgtcatcg gattgtcggg aggtgttgac tccagtgttg ccgcctgggt
    15541 tctgaaaaat gaaggctatg aagttgaggc ccttttcatg agaaactgga atgataccac
    15601 cggtttgctg aaaagtgact gtcactggga agatgatctt atgttcgctg aaatggtggc
    15661 aaagaagctg aaaattccat ttcgttttat cgatctgagt gaatcgtaca gaaaacgggt
    15721 ggccgactac atgttcagcg agtatgaaaa aggaagaaca cccaatcccg atgtactctg
    15781 caacagggaa atcaaattcg attcctttgc ccgggaggct ctgaaaacag gtgctgatta
    15841 catggcaacc gggcactatt gccgcaagga tgccattaaa agggatggca aaatcatatt
    15901 ccgcctgctg tcgggcatgg acaacaacaa ggaccagagt tattttctat gccaggtcag
    15961 ccagaagcag ctggccaagg cgctgttccc cataggcgaa atgacaaaac cccgggtacg
    16021 tgaaatagct gatgaactta agcttcctac agcccggaga aaagattcac agggcatctg
    16081 cttcatcggt aaggttcacc tgccaacctt tctgaaacaa aaactcaaat cagagaaagg
    16141 tgaagttatc aggatcccgg ccgaaaaatg cattacccgg tggctcaggg gaattcccgg
    16201 ccccgagctt gatgacagcc tactcctgat gaataccacg cccccggttt acagccctga
    16261 aacgggttct gtcgcggggg tgcatgaagg agctcacttc ttcaccatcg ggcagcggaa
    16321 gggattgaat ataggcggat caaaagagcc gcttttcgtc atcggtacag acactttaaa
    16381 caacattgta ttcgtgggag aaggcgaggc tcatcccggg ctttaccgga aagggttgag
    16441 gatcagacgg gacgatgtac actggattcg aaaggatctt gagctgaatg aaaatgagtc
    16501 tcgtttatac atggtcagaa taagatacag gcagccgctc cagaaagcca ggttgtacag
    16561 gagaaataat gatattcaca tcattttcga cagattgcag cggggcatta ctgcagggca
    16621 gtttgcagca tggtatgacg gcgatgagct tatcggatcg ggggcaattg acggttaaaa
    16681 ggtaagcctg cctgtcagct taaccttccc ttgtaatcct catatccgaa ctgacgtaac
    16741 agccggaagc tgttatcagc agtccaaatt ccgatagacg gatgctgtac cccgttgaag
    16801 gttgttgtct tgaccatggt ataatggatc atgtcgagga atattaccct gtcacccggc
    16861 cgcaggggct tctcaaacga ccagtctccc atcacatcac ccg
//
LOCUS       DAOOYG010000053        16839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3329_length_16839_cov_7.508222, whole genome shotgun sequence.
ACCESSION   DAOOYG010000053 DAOOYG010000000
VERSION     DAOOYG010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16839
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3329_length_16839_cov_7.508222"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PK288_06280"
     CDS             complement(<1..364)
                     /locus_tag="PK288_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18563.1"
                     /translation="MKKIFTLISASFFTVCLFAGGLVTNTNQSASWVRLPARDASTGI
                     DAVYFNPAGLMKLDNGLHLSLSNQTIFQNKEVENFYKGPGGSFGLNQSLYKGKVKAPL
                     FPSIYAAYKLDKLAFSIGF"
     gene            complement(603..3728)
                     /locus_tag="PK288_06285"
     CDS             complement(603..3728)
                     /locus_tag="PK288_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPM18564.1"
                     /translation="MKLRSASLIIILLPVTFTCLDSCRKRSVNEITINDFATLSKEFR
                     QPAAEYTTAPFFVWNYKITKEEIDSYLNEFNRQGSKQVFIHPRPGLITEYLSDEWFEL
                     CRHTVDKAGELGMKVWLYDENSYPSGFAGGHVPDRVPESYNQGQGLAMTRFTSLPDTA
                     DRFFLCLKEESGQFEDITSRLGDEKGKTGSYLLFSKTYNRKSDWYGGFSYVDLLCPGV
                     TEEFIKITMTGYKKHLGREFGRIVPGIFTDEPQIESPGGIRWTPDLFDVFEKQWNYDL
                     RTHLPSLFEETGDWKKVRHNYTQTLLNLFIDRWAKPYFRYCEKNNLKFTGHYWEHEWP
                     NMNNGGDNMAMYAWHQVPAIDMLFNQWNDSTPRGQFGNVRSVKELASAANQTGRQRKL
                     SETYGGAGWDLTFTDMKRLGDWEYVLGVNFMNQHLSHITLAGARKYDYPPTFTYHEPW
                     WNNYRYINDHFARLSVALSSGEQVNKILILEPTTSAWLYDSYTNKNPKMGEIGQSFQS
                     FITRLEKEQVEYDLGSENMIKNLGSVKKAKLVIGHRAYPVVVIPPMMENLDLTTFRIL
                     KRFVSEGGKLVAFSIPTLVAGSSNNDLAGFFSGTANISAVKKPDQETIKTYFSDPEIS
                     FLNVKGGNLYHHRRILSDGQLVFLTNSSLTESTSGLLKIRGKGAIEFNTLSGEITGYP
                     CTDDGDHIRLPFNLYPAGSLLLFVPAMKPDGFSAPPAPGNPEVVPSDSQMKVIREEDN
                     AFPIEFCDIIAGGESATDLNTYDAADKIFRIHGFANGNPWNTSVQFKTNIVDRDTFGI
                     TSGFIATYRFEVKGTFDCSSIMAIIERPYLWKTTVNGKEIVPEPGRWWLDRSFGVFKI
                     GPMINQGENTITLTASPMRVHAEIEPVYLTGDFSVKPGAKGWYLEAPVNNLTTGSWKD
                     KGLPFYSWGITYRKEYDIEKISGKYFVSMGKWNGTIAEVKVNGREAPLIAFPPYRSDI
                     SDLLVPGTNTIDVTVTGSLKNLLGPHFNNPAPGLASPWHWRNVKIYPPGNEYQLHDYG
                     LMEEFILVHSD"
     gene            complement(3888..5321)
                     /locus_tag="PK288_06290"
     CDS             complement(3888..5321)
                     /locus_tag="PK288_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose isomerase"
                     /protein_id="HPM18565.1"
                     /translation="MKDIYRLKIGLFGIGLDTYWPQFIGLKERLEGYLALVASRLRKY
                     DSEIINLGLIDNPADALRAGHDFRKADVDIIFLYVTTYALSSTVLPVVRRAKVPVIIL
                     NLSPEKAIDYSAFNKLGDRTKMTGEWLANVSACPVPEIANVFLRSGIKFHQITGVLQG
                     DEDVWKEAGEWIEAASVANIMAHNRLGCMGHYYGGMLDVYTDLTLHLSVLGGHIEMLE
                     VDELSALRKEVTDAEISERVSMFHKEFEIDRGCPEEDLKNAARTSVALGKMVQAHDLG
                     SLAYFYSGSGIRENEESISTIILANSLLTARGVPVAGEYEIKNIHAMKIMDSFGAGGS
                     FTEFYALDFNDDVILMGHDGPGHLAIAEGKIKVKPLEVYHGKVGKGLSVEMSVKHGEI
                     TLLSLVQKPDGRLMFLVAEGESVPGPILEIGNTNSRYRFPTGLKNFVNDWNKYGPAHH
                     CAAGVGHIASKISKLGELLDIEVVKIC"
     gene            complement(5456..7675)
                     /locus_tag="PK288_06295"
     CDS             complement(5456..7675)
                     /locus_tag="PK288_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM18566.1"
                     /translation="MTASAIQAAVSDGVVRGKVTDKSTGEALPGVYVLYGKNQGTTSN
                     DKGYYIFSTQAEKLTIVFQLVGYGALTREISFSRRDTVTLDVSLEMKIREIDQIVVSA
                     GKYEQKISELTVSMDVIRPALLTSNHITDAQEMLNKTPGIEVMDGQASVRGGSGFSYG
                     AGSRVMALIDGLPFLSADAGNIKWQFLPLENISQVEIIKGASSVLYGSSALNGIINFR
                     QADATNIPETRFYTETGIYGSPRNRNWKWWNSPRFSSSASLSHLRKLGNTSIGLSASV
                     LYDNGYRRLNDEKLARIGIRLKHNSSKLKGLSYGVNLNSGFTKAQDFVLWEDGYTGAL
                     KQSESTAITLHGDFLSVDPFVSYSGSGRTRHELKVRLQSTRNRFPDSEKNNSDAVSFY
                     SEYQGSLKPGEKTGLTFGICESYGKINSEFFGDHTSLNLAGYAQAEYRPVNRLKAAAG
                     LRLENYFLDGIADKLVPVFRTGLNLQAASYTFLRLSFGQGYRFPSIAEKFASTTLGSV
                     KIFPNPGVQPETGWNSEIGIKQGLSIGNISGQADFAAFLTRNNRLIEYIFSYYQDPVT
                     GIPDFGFMATNVEQSRILGFETEFLLNIPAGNVNTTVTAGYTWLSPVGYNRITHKTTG
                     VYLKYRRKHSASLNIASSWEKFDFGLSLYAKSKILNIDDVFLDPGTREEILPGFYDYW
                     TRNNKGYFTLDGSLGYKITPRLSLSFVVKNFTNTEYMGRPGDIQPHRNFSVRLTGNY"
     gene            complement(7705..8112)
                     /locus_tag="PK288_06300"
     CDS             complement(7705..8112)
                     /locus_tag="PK288_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18567.1"
                     /translation="MRNLFFLLVITGLLVNFSCKKTESERFTFLTGAIWVTDSLVANG
                     QDAGGPGQLLADFSGEARFNKDGTGSFGNYTGEWSFSSDETQLVIKTNAIAFPVTADI
                     KELTKVSLKLTTVMPNPMDIQNPYRIRMTFKAK"
     gene            complement(8182..9345)
                     /locus_tag="PK288_06305"
     CDS             complement(8182..9345)
                     /locus_tag="PK288_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPM18568.1"
                     /translation="MSTKKINRRMFVRTTALTAAGLSIGAANAFPSGTGAVRNKLPKW
                     KGFNLLDIFSPYPPKGPDPSKTTEDDIKWMADWGFDFVRIPMAYPRYVLFDRSRDITK
                     DEVYKFDEKVLEEIDKLISTAHKYNMHVSLNLHRGPGFCINAGFHEPFNLWKDAEAQD
                     AFNFHWSMWAKRYKGIPSGKLSFDLLNEPLYREDMNDQFSKSSPLPGELYRKVAKGAA
                     DAIRAVDPDRLIVADGNGGGSYVTPELIDLNIAQSCRGYYPHYISHYQASWVWKDPAM
                     APRPVWPGIIDGKQFGRKSLEDFYAPWIELLEKGVGVHCGECGCYSKTPHEVFLAWFT
                     DVIDILTSHGIGFALWNFRGTFGIMDSDRSDINYTDWYGHKLDSKLLDLIRKY"
     gene            9422..10675
                     /locus_tag="PK288_06310"
     CDS             9422..10675
                     /locus_tag="PK288_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPM18569.1"
                     /translation="MNRFIMNSRERLVTAISHREPDRIPVDLGATPSSGISAVAYHNL
                     ILHLGLGLENHVYDVVQEVTQPEMELLDLFRVDVLDIGRFFNSHESYWHRLELINGIP
                     GLYPKWFNPQRQEDGSWLASGPTGEFIAKMPAGATFFDQTIFPYADGYPDNFYDLEHA
                     MSRVSWGGFGFPPFDRITEEDFWSNLRTTLKKVRASTDRALVLGAGCNLFEWGTFLRR
                     IDNFLMDLYLDPDNVNKLLDALMERHMDFLSKVCQSVGDIVDIIKFGDDLGTNTGPFM
                     PPEIYDRFFKPRHRMLCEYVKANSTAYTMLHCCGGIYELIPGLIDAGFEILNPVQINA
                     FGMDPARLKKEFGSSLTFWGGGANTQSVLNKASAVEVRKHVRENIRILSKGGGFVFNT
                     VHNIMPDVPPGNIVAMFEAVREFDR"
     gene            complement(10767..11888)
                     /locus_tag="PK288_06315"
     CDS             complement(10767..11888)
                     /locus_tag="PK288_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPM18570.1"
                     /translation="MEKGHNIHSERLYSLDALRGFDMFWIMGGAGIFTGLATLTNWAP
                     LNWWADQLKHVQWQGFRFYDLIFPLFLYIAGISFPFSLAKRTALNQSRKSIYRHVIFR
                     GLILVFLGILYNNAVRFNLAGLRYGSVLGRIGLAWMFAAIIYMNTKLTLRIVWFWVIL
                     AGYWLLLLLFPAHDLGSSDPFSQTGNLASYIDRLLMPGKLLLDNHDPEGLLSTVPAIG
                     TALLGMLTGDFMRSGYQADKLIRKVTIMVIASVFLIVLGKIWDTVFPINKNIWSSSFV
                     CFAGGLSLLLFAIFYLVIDVWKFRKWAFFFVVIGLNPITIYLANRIINFRHAADFFFG
                     GFTAILPETWHGLIGGIGLTTIGWLFLYFLYKKKIFLKV"
     gene            12158..13345
                     /locus_tag="PK288_06320"
     CDS             12158..13345
                     /locus_tag="PK288_06320"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM18571.1"
                     /translation="MPGPGAFWFGKEETDAVIEVIRSGYLFRYGSENDPSFLHKTATL
                     EREFARYSNAGYALATSSGTASLLVSLLAAGLEPGDEIIVPAYTFVASYSSAIFAGLV
                     PVLAEIDESLTLDPVDIESRITPRTRAIMPVHMLGNPCNMDAVMKIAEKHGLLVIEDA
                     CQSAGASYKGKKAGTIGNIGAFSLNIFKTINSGDGGIVITSDKDLYERAFAIHDQGHK
                     PMRFGVEVGSRNILGLNFRMNELTAAVSLAQLQKIDRIISTLREKREKFKKLISPAKG
                     FRFRRLNDPDGDCATLCSVIFDTREQAARVAGSLGSKTVDQSGWHVYSNMEHVLSHLK
                     KIGQPHTRGAYPRTDDILGRTINISVGVVDGGLGSGWGININSTDDQIEAAAEQFIAA
                     CEN"
     gene            13499..14830
                     /locus_tag="PK288_06325"
     CDS             13499..14830
                     /locus_tag="PK288_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18572.1"
                     /translation="MATRRVFVKKASLGAASIVFSAKSYSRIVGANDRVRVGIIGYSD
                     RFKGSLSPSFLSLSKEMNFEFTVISDIWRLRREEADRFYKSMGINLRLARNNDELYEK
                     NDTDAVIISTADFQHALHCAEAVGAGRDAYVEKPFAETMADNRTALKAVQNSGKIVQI
                     GSQRRSAPNYIAANEYIKSGKFGKIVMVEMTWNVNQPGRWRRPNVVPTLKEADTDWKR
                     YLMNRPLTAFDPRRYLEFRLFWPYSSGIPGQWMAHQIDTVHWFTGLGHPRSVAANGGI
                     YLWKDGRTNFDTMTAVFDYGNNADPNSGFQVVYSSRFTNSAGGVKELYFSNGGMLNLD
                     TNRITSEGGLEESDASAMNMKPNLLKTFELPSVSIETAADTGGDTMTIAHMKNWMECV
                     RSRKKPNADVTAGYNHSIANIMCTASLRTGEKATFDEKNQEVLAGGKIFQY"
     gene            14859..15656
                     /locus_tag="PK288_06330"
     CDS             14859..15656
                     /locus_tag="PK288_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HPM18573.1"
                     /translation="MKRSVLSVAFAVLAAGTLLYSCRSSATPDEKQMYYELRIYRLKD
                     PGKNDLVDRYLKNAFIPAMRKAGIQAVGVFRPVETDTAYGKMVYVFIPYKNMDQYSGA
                     LEILEKDPDHQKAGREFLDAPYNDPAFIRYESIFMKAFAFMPVYRKYTFDTPPEERIY
                     ELRSYESWTEVKAMKKIQMFNEGGEIAIFDEIGSNAVFYGQVLFGSLKPRLMYLTTYS
                     NMQSRDEHWNAFRAHPVWKKISVMEEYANTVIRPDAYLLHPAPYSDF"
     gene            complement(15824..>16839)
                     /locus_tag="PK288_06335"
     CDS             complement(15824..>16839)
                     /locus_tag="PK288_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPM18574.1"
                     /translation="AIAQAYYNKKEGIKRITTETGAGQWGSALSYACNHFGLELLVFM
                     VRVSYTQKPGRKLLMNSYNAKVVPSPSTMTEAGRKILEKDPDSPGSLGIAISEAMEIA
                     VQDPYTKYSLGSVLNHVCLHQTIIGQEALLQMEKAGDYPDVVIGCFGGGSNFSGIAFP
                     FIRENIVNDRKTRFVTVEPSSCPKLTKGKFMYDFGDTAGMTPLLPMFTLGHNFVPEKI
                     HAGGLRYHGAGVLVSQLLKDGFIEAKAKMQRECFEGGVLFARTEGILPAPESNYAIAG
                     ALDEARKADLEGISKTILFNLSGHGHFDISSYEKYFEGNLPDHEMTSAEVEATISKIE
                     LPD"
BASE COUNT         4381 a         3834 c         3985 g         4639 t
ORIGIN      
        1 tgaatccgat tgagaatgca agcttgtcga gtttataggc agcatatata cttgggaaca
       61 gcggagcttt caccttgcct ttgtacaggc tctgattcag tccgaatgat cctcccggcc
      121 ctttataaaa attttcaact tccttgttct ggaagatggt ctggttggaa agtgataaat
      181 gcagtccatt atccagtttc atcaaaccgg caggattaaa gtaaacggca tcgatgccgg
      241 ttgatgcatc cctcgcagga agcctgaccc aggaggcact ctggttggtg ttggtcacca
      301 gtccgccggc aaagagacag acggtgaaga acgaagcaga aataagagta aaaatttttt
      361 tcatcggatc atttttaagt ttgtaactag gaaacacagt cacttttgac cgttactgtt
      421 attttcctga tttttaatat gatttgtttt caggttcaaa gaacaaaagc agggcaaatg
      481 tagaaaaata tttgaattat gcaaataagt ccaaaatacc aaattgatat taagatattt
      541 gtgaccccga tttgatgttg ttcgaaaaat aacactgccg gtactacggc actataccgg
      601 aatcaatcac tgtgaactaa aatgaactct tccatcaggc cataatcatg aagctggtat
      661 tcattaccgg gagggtagat ttttacattt ctccagtgcc agggcgaggc caggccggga
      721 gccggattgt tgaaatgcgg tcccagcagg tttttaaggc ttcctgttac tgtaacatca
      781 atggtattgg ttcctggtac gagaaggtct gatatgtctg acctgtaggg aggaaaggct
      841 attaggggtg cttcccggcc gttaactttt acctcggcaa tggttccgtt ccattttccc
      901 attgaaacaa aatattttcc ggagattttt tcaatatcat attcttttct gtaggttatt
      961 ccccacgaat agaacggaag tcccttatct ttccagctgc cggttgttaa attattaaca
     1021 ggagcctcaa ggtaccatcc ctttgctccg ggctttacgg agaagtcacc cgtcaggtag
     1081 acaggttcaa tctcggcatg tactctcatt ggtgatgcag taagggtaat tgtattctct
     1141 ccctgattaa tcattggtcc tattttaaaa acaccaaagg atctgtcgag ccaccatctg
     1201 cccggttcag gaactatttc cttaccattc actgtagttt tccataggta gggtctttca
     1261 atgattgcca ttattgatga acaatcgaaa gttcccttaa cttcaaaccg gtatgtggcg
     1321 ataaagcctg atgtaatccc gaaggtgtcc cggtcaacaa tgttagtttt aaactgaacc
     1381 gatgtgttcc agggattgcc gttggcgaag ccgtggatcc tgaatatttt atcggctgca
     1441 tcataggtgt tgagatctgt tgcagattcg ccacctgcaa taatatcaca gaattcaatc
     1501 gggaaagcat tgtcttcttc cctgatgact ttcatttgtg aatcagaagg aacaacttcc
     1561 ggattcccgg gtgccggagg tgcggaaaaa ccatcaggct tcatggccgg aacaaaaagg
     1621 agaaggctgc ctgcaggata aaggttaaat gggagcctga tatggtcgcc atcgtctgtg
     1681 cagggatatc ctgttatttc acctgaaagg gtattgaatt cgatggcacc ttttcccctt
     1741 attttcagta aaccggaggt tgattcggtg agacttgagt ttgtaagaaa aacaagttgt
     1801 ccgtcggata atattcttct atggtgatag agattacctc ccttaacatt caggaaagaa
     1861 atctccggat ctgaaaaata ggttttgatc gtttcctgat cgggtttttt aacagctgaa
     1921 atattagcag tcccggagaa gaaccctgcc agatcattgt ttgatgatcc ggctaccagg
     1981 gtcggtatgg aaaaggctac gagttttcct ccttccgaga cgaatctctt caggatcctg
     2041 aaagttgtaa gatccagatt ttccatcatg ggcgggatca caacaaccgg ataggcccga
     2101 tgaccgatta ccaactttgc cttttttacc gatccgaggt ttttaatcat attttccgat
     2161 ccgagatcat attcaacctg ctccttctcc agtcttgtaa taaaggactg gaatgattgt
     2221 ccgatttctc ccatttttgg atttttattg gtatatgagt catagagcca ggcagatgta
     2281 gtgggttcga gaataaggat cttgtttact tgttcgccgg aggacaatgc aactgataat
     2341 cttgcaaaat ggtcattgat gtatctgtaa ttgttccacc atggttcatg ataggtgaaa
     2401 gtgggcggat agtcatattt ccgggcccca gccagggtta tatgggaaag gtgctgattc
     2461 atgaaattga cgccgagcac atactcccag tctccaagtc ttttcatatc ggtgaaggtg
     2521 aggtcccacc ctgcgccgcc gtatgtttcg ctcagtttcc tttgcctgcc ggtctgattc
     2581 gcggcgcttg cgagctcttt gaccgatctt acattgccga actgccccct gggtgttgaa
     2641 tcgttccact ggttgaacag catatcgatc gcaggcacct ggtgccacgc atacattgcc
     2701 atattatccc cgccgttgtt catgttcggc cactcatgct cccagtaatg ccctgtgaac
     2761 ttgaggttgt tcttttcgca ataccggaag tacggttttg cccagcggtc aataaaaagg
     2821 ttcagaagag tctgggtata attatgccgt acctttttcc agtctcccgt ctcctcaaaa
     2881 agcgagggca gatgagtacg gagatcataa ttccactgtt tttcgaaaac atcaaacaga
     2941 tccggtgtcc atcttatacc acccggagac tcaatctgtg gttcatcggt aaagatgccg
     3001 ggaacgatcc gtccgaattc cctgccgaga tgttttttat acccggtcat tgttatttta
     3061 atgaattcct cagttactcc gggacagagg aggtcgacgt aggagaagcc tccgtaccag
     3121 tcgcttttcc tgttgtaagt tttactgaaa agcagatagc tgccggtttt acccttttcg
     3181 tcaccaaggc gtgatgtaat atcttcaaac tgacccgact cctctttcag gcacaggaag
     3241 aacctgtcgg ccgtatccgg aagactggtg aatctggtca tggccagtcc ctgtccctgg
     3301 ttatatgatt ccggaaccct gtcaggaaca tgtccgccag caaaaccgct gggataagag
     3361 ttttcatcat ataaccagac tttcattccc agctccccgg ctttatcaac cgtatggcgg
     3421 cagagttcaa accattcatc agaaaggtat tcggttatca ggccgggccg cggatgaatg
     3481 aaaacctgtt tgctgccctg cctgttgaat tcattcaggt aactgtcgat ctcttctttg
     3541 gtgatcttat agttccacac aaaaaaagga gctgttgtat attctgcggc tggctgcctg
     3601 aattcctttg aaagagtagc aaaatcattg attgttattt cattaacact tctctttctg
     3661 caagaatcaa gacaggtaaa ggtaacaggg agaagaatga taatcaggct ggcggatctt
     3721 aatttcatca ctgacagggt ttgatttgat aatgtttgta ttttgttaaa ggttagtcat
     3781 tcagatgttt agcaagatat tgcagagtgt ggcaattatt gttattaccg atagtttgca
     3841 tttgatttcc ctggcacctg gttcctttca accggtatac gtcaatctca gcatattttc
     3901 accacttcga tatccagcag ctctcccagc ttactgatct ttgaggcaat atgtccgaca
     3961 cctgccgcac agtgatgagc cggtccgtat ttgttccagt cattaacaaa atttttcagg
     4021 cctgtcggga atctgtacct gctgttggta ttgccgattt ccagaatcgg ccccggtacc
     4081 gattcgccct cagcgacaag gaacatcagc ctgccgtcgg gtttttgtac aagtgacagc
     4141 agggtgatct ccccgtgttt cactgacatc tctaccgaca ggcccttccc gaccttcccg
     4201 tggtacactt caagcggttt tacctttatc ttcccttcgg cgatggccag atgtccggga
     4261 ccgtcgtgcc ccatgagtat gacgtcatca ttgaaatcga gggcgtaaaa ttcggtaaac
     4321 gatcctcctg ctccaaagct gtccattatt ttcattgcat ggatattctt tatctcatat
     4381 tcacctgcaa ccggaacgcc cctggccgta agcagggaat tggcaagaat aatggtgctt
     4441 attgattctt cattttccct gatgcctgag ccgctgtaaa aatatgccag ggaacccagg
     4501 tcatgagcct gaaccatttt tcccagggcc acggaagtgc gtgctgcatt tttcaggtct
     4561 tcttccgggc atcccctgtc gatctcgaac tccttatgga acatacttac ccgttcgctg
     4621 atttcagcat cagttacttc cttgcgcagt gcggagagtt catccacctc aagcatctcg
     4681 atatgacctc ccaggactga aaggtgcagg gtgaggtcag tgtacacatc cagcattcct
     4741 ccatagtagt ggcccatgca tccgagcctg ttatgcgcca ttatgtttgc aaccgaggct
     4801 gcctctatcc attctcctgc ttccttccat acatcttcat cgccctgaag tacacccgtt
     4861 atctggtgaa acttaattcc cgacctgagg aatacgtttg ctatttccgg aacagggcag
     4921 gcagaaacat tagccagcca ttcgcctgtc atttttgtcc ggtcgcccaa tttgttgaaa
     4981 gcactgtaat caattgcctt ttcgggtgac agattcagta ttatcaccgg cacttttgct
     5041 cttctaacca cgggaagtac ggttgatgaa agggcatagg tggtgacgta cagaaagata
     5101 atgtcaacat cagctttccg gaagtcatga ccggcgcgca gggcatcagc aggattatca
     5161 ataagcccca ggttgattat ttcactgtca tatttcctaa gccttgaggc gaccagggcc
     5221 aggtatccct cgagtctttc tttcagcccg atgaactggg gccagtatgt atccagacca
     5281 atgccaaaaa gaccgatctt cagcctgtaa atatctttca tgtttctttg tttaatatta
     5341 tgatcaggct tcgcctgtat cagttcagtt cattaataac agccccggat cccggaaatt
     5401 ccatgaaata aaaaatatgc ccgttgtttg atttcagccg ttccggaaat attcatcagt
     5461 aattccccgt cagtctcaca ctgaaattcc tgtgcggctg aatatctccc ggacgtccca
     5521 tatactccgt gtttgtgaaa tttttaacaa caaaagagag gctgaggcgg ggagtgattt
     5581 tatatcccag gctcccatca agcgtaaaat atcctttgtt gttccgtgtc cagtaatcat
     5641 aaaaaccggg caatatttct tcccttgtac caggatcgag gaaaacatcg tcgatgttga
     5701 ggattttcga ctttgcataa aggctcagtc cgaaatcaaa tttttcccat gaacttgcga
     5761 tattcaggct tgccgaatgt ttcctcctgt acttcagata gacgccggtg gttttatggg
     5821 tgatcctgtt gtacccgacg ggggatagcc aggtgtaacc tgcggttaca gtggtattga
     5881 cattcccggc ggggatatta aggaggaatt cggtttcaaa tcccagtatc cgtgattgtt
     5941 ccacattggt tgccatgaag ccgaaatcag ggatgcctgt gaccggatcc tggtaatatg
     6001 aaaaaatata ctcaatgagc ctgttgtttc tggtgaggaa agctgcaaaa tcagcctgcc
     6061 cgctgatatt tccaatactt aatccctgtt ttattcctat ttccgagttc caccctgttt
     6121 caggctgaac gccggggttc gggaagattt taaccgaacc gagtgttgtt gatgcaaatt
     6181 tctctgctat tgaagggaag cggtaaccct gtccaaatga taacctgagg aaagtataac
     6241 ttgctgcctg aagattcaga cctgtacgga aaacagggac cagtttgtct gcaatgccgt
     6301 cgagaaaata attttcaagt ctgagtcctg ctgctgcctt aagccggtta accggcctgt
     6361 attcagcctg ggcatatcct gcaaggttca ggcttgtatg gtcgccgaaa aattcagaat
     6421 tgatctttcc gtacgattcg cagatgccaa atgtcagacc tgttttttcc ccgggtttca
     6481 gtgacccctg gtactcggag tagaaggaaa ctgcgtcact gttgtttttt tcgctgtcgg
     6541 ggaacctgtt ccttgtagac tgcagcctga ctttcaactc gtgacgggtc cggcccgatc
     6601 cggaatagga gacaaacggg tcgacggaca ggaaatcccc gtgaagcgtg attgcggttg
     6661 actcactttg cttaagggct cccgtgtacc cgtcttccca caggacgaaa tcctgcgctt
     6721 tcgtgaatcc cgagttcagg ttcactccgt aactcagtcc tttcagtttt gaactgttgt
     6781 gtttcagcct tatgccgatg cgggcaagct tttcgtcgtt aagacgcctg taaccgttat
     6841 cgtaaagaac agatgcactt aatccgatac tggtgtttcc gagcttcctc aaatgggaga
     6901 gggaagctga cgaagagaat ctgggcgagt tccaccattt ccagtttctg tttctcgggc
     6961 tgccataaat ccctgtttcg gtgtagaatc ttgtttcagg gatgtttgtt gcgtcggcct
     7021 ggcggaagtt gattattccg ttcaatgctg atgaaccata gagaacagat gaggcgccct
     7081 ttataatttc aacctggctg atgttttcga gcggcaggaa ctgccatttt atgttgccgg
     7141 catcggcaga aaggaaaggc agcccgtcga taagtgccat aaccctgctt cctgctccat
     7201 agctgaatcc gctgccgccc ctgacggaag cctgaccgtc catgacttca atgcccgggg
     7261 tcttgttcag catctcctgt gcgtcggtaa tatgatttga agtgagaaga gcaggcctga
     7321 ttacatccat tgaaacagta agctcagata tcttttgctc atattttccc gcgctgacaa
     7381 ctatctgatc aatttctctg atcttcattt cgagactgac gtcgagggta acggtgtctc
     7441 tccttgaaaa ggagatttcc ctggtcaggg ctccgtagcc gacgagctga aacactatgg
     7501 taagtttttc agcctgagtg gaaaaaatat aataaccttt atcattactt gtcgttccct
     7561 ggtttttgcc atacaggaca tagacacccg gcaaagcttc accagtactt ttatcggtta
     7621 cttttcccct gacaacacca tctgatacag ccgcttgtat cgctgatgca gtcaaaagga
     7681 aaattgccgt aatgatgcac cgggtcattt tgccttgaat gtcattctga tcctgtaagg
     7741 attctgaata tccatagggt tcggcatcac ggttgtcagt ttcagggaga cttttgtgag
     7801 ctcttttata tctgctgtta ccggaaaggc aatagcgttg gtttttatta caagctgggt
     7861 ttcatcgctg ctgaaactcc actctcccgt gtaatttccg aaacttcccg ttccgtcctt
     7921 gttgaacctg gcttcaccac taaaatcggc aagcagctga ccgggtccac cagcatcctg
     7981 tccgtttgca accaggctgt cggttaccca tatggcgccg gtcagaaaag tgaatctttc
     8041 cgattccgtc tttttgcagg agaaattcac gagaagtccc gttattacca gtagaaaaaa
     8101 caaatttctc atcgcagtgt gattttactt tttaaaaaga tcagtcaatc atattaacgg
     8161 gataatgccg attgcattcc ctcagtattt tcttataagg tcgagcagtt tactgtcgag
     8221 cttatggccg taccagtcgg tatagtttat atccgatctg tcagaatcca ttattccgaa
     8281 ggtacccctg aagttccaca gtgcgaagcc gataccgtgt gacgtgagaa tatcgatcac
     8341 gtcggtgaac caggccagaa aaacctcatg gggtgttttg ctgtagcatc cgcactcacc
     8401 gcagtgaaca cccacccctt tttccaggag ttctatccac ggtgcataga agtcttcaag
     8461 cgacttgcgt ccgaactgtt ttccgtcaat gatcccgggc cagacgggtc tgggtgccat
     8521 tgcaggatct ttccacaccc acgaggcctg gtagtgggaa atataatggg gataatatcc
     8581 ccggcagctt tgggcaatat taagatcaat aagttcagga gttacatagc tccctccgcc
     8641 gttgccgtcg gcaacaatga gacggtcggg gtcaacggcc ctgattgcat cagctgcccc
     8701 tttggcaacc tttctgtaaa gctcgcccgg aaggggagag ctttttgaga actggtcatt
     8761 catatcctcc ctgtataatg gctcattcag cagatcgaag cttaactttc cggaggggat
     8821 tcccttatat ctctttgccc acatgctcca gtggaaatta aaggcatcct gtgcctcggc
     8881 atccttccag aggttaaatg gttcatgaaa tcccgcgttt atacagaaac ccggtccgcg
     8941 atgcaggttg aggcttacat gcatattata cttgtgggct gtggaaatta gtttgtcaat
     9001 ttcttccagc accttttcat cgaacttgta aacctcgtct tttgttatgt ccctcgatct
     9061 gtcaaacaag acatatctgg gataagccat tggtattctg acaaaatcaa aaccccagtc
     9121 tgccatccat tttatatcgt cttcggttgt cttcgacgga tcggggcctt tcggcggata
     9181 aggtgagaaa atatccagca ggttaaagcc tttccatttc ggcagcttat ttctgactgc
     9241 cccggttccg gacgggaatg catttgcggc accgatggaa agaccggcgg ctgtcagtgc
     9301 agtggttctg acgaacatgc ggcgattgat ttttttcgtg ctcataatat gacggattat
     9361 ttatttgtca gtgaatactt ctcccaaatt aacaattatt taataaattc atagtcaaat
     9421 aatgaatcga tttatcatga attccaggga aaggcttgtc acagccatat cgcacaggga
     9481 gccggaccgg atacctgttg atctgggagc cactccaagt tcaggtattt cagctgtggc
     9541 gtatcataac ctgatcctgc atcttggcct tggccttgaa aaccatgttt atgatgttgt
     9601 tcaggaagtc acccagcctg aaatggagct gcttgatctc ttcagggtgg atgtgcttga
     9661 cataggcagg tttttcaaca gtcatgaaag ttactggcac aggctagagc tgatcaatgg
     9721 catccctggc ctctatccga aatggtttaa tcctcagcgt caggaagacg gaagctggct
     9781 cgcatccgga cccacaggtg aattcattgc aaaaatgcct gccggggcca ctttctttga
     9841 tcagaccatc ttcccatatg ctgacggata tcctgacaat ttctatgatc tggaacatgc
     9901 catgtcgcgg gtaagctggg gaggcttcgg ttttccgccc tttgaccgga ttaccgaaga
     9961 ggatttctgg agcaatctga gaaccacatt gaaaaaggta agagcatcaa ccgacagggc
    10021 tttggtgttg ggagccggat gcaatctttt cgagtgggga acctttttgc ggaggataga
    10081 taattttctt atggatctgt accttgaccc ggacaatgtc aataagctgc tggatgccct
    10141 gatggagagg catatggact tcctttcaaa ggtgtgccaa tcggtagggg atatcgttga
    10201 cataataaaa ttcggagatg atctgggtac caataccggt ccgttcatgc cgccggaaat
    10261 ttatgaccgg tttttcaaac cccggcacag aatgttatgt gagtatgtaa aggcgaacag
    10321 tactgcatac acaatgcttc actgctgcgg cgggatctat gaacttatac cgggtctgat
    10381 cgatgcagga ttcgagatcc tgaatcccgt gcagataaac gcctttggaa tggaccctgc
    10441 ccgtctcaag aaagaattcg ggagcagcct gacattctgg gggggcggag caaataccca
    10501 gtcggtgctc aataaagcct ctgccgttga ggttagaaaa catgtaaggg agaatatcag
    10561 aatcctgtcg aaaggaggag gatttgtttt caatacggtt cacaatataa tgcctgatgt
    10621 tcctccaggg aatattgtgg caatgttcga agctgtcagg gagtttgacc ggtaagcagc
    10681 cgggcttgct gcaataattg tccggggagg atattcctct tgatttcatc ggggactgaa
    10741 cgaagcaggc aggccgtttt gggtcattat actttcagga agatcttctt tttataaagg
    10801 aaatagagga acagccatcc aatggttgtc agtccgatcc ccccgatcaa cccatgccat
    10861 gtttcaggca ggatcgcagt gaatccgccg aagaagaaat ccgcggcatg tcggaagtta
    10921 atgatcctgt ttgcaaggta gatcgtgatg ggattcagcc cgataactac aaagaagaaa
    10981 gcccatttcc gaaatttcca tacatcaatc accaggtaga aaattgcgaa aagcagcagg
    11041 ctcaatcctc ctgcaaagca cacgaatgaa ctgctccaga tgtttttgtt tatcgggaac
    11101 actgtgtccc agatttttcc cagtacgatt aaaaaaactg atgcaataac catgatcgtt
    11161 actttgcgga tcagtttatc ggcctggtat ccggatctca tgaagtcgcc ggtcagcatg
    11221 cccagaagag ctgtcccgat ggcagggact gtactcagta gtccttccgg gtcgtgattg
    11281 tccagtaaca gttttcctgg catcagcagg cggtcgatat aactggcaag gtttcccgtt
    11341 tgagagaaag gatctgaaga cccgagatca tgagcgggga aaagcaggag caacagccag
    11401 tatccggcaa ggatgaccca gaaccagaca atcctgagag taagttttgt gttcatatag
    11461 atgattgcgg caaacatcca tgcaagcccg atccggccaa ggacactccc atagcggagc
    11521 ccggccagat tgaacctgac ggcattattg taaaggatac caagaaaaac aagaatcagt
    11581 ccccggaaaa tcacatgccg gtaaattgat ttccggctct ggttcaatgc agtcctcttt
    11641 gccagtgaaa atgggaatga aattccggct atatacagga aaagcggaaa gatcaaatca
    11701 taaaacctga aaccttgcca ttgcacatgc ttaagctggt ccgcccacca gtttagcgga
    11761 gcccagtttg taagggtggc aagaccggtg aatatgcctg caccgcccat gatccagaac
    11821 atatcaaacc cgcgtagtgc atcgagggaa taaagccttt ccgagtggat attgtgtcct
    11881 ttttccattt ctgtgttatt aagttcaatt atgttgaaat gatgatgcaa taatcagaaa
    11941 acaaaggaaa taatatttta aatgaatatt gatactgcct ttaaaagaaa ataggaaaag
    12001 aatactaact ttgagatcag gcgcatttgt atttaattcc agcctcaggc tgacataaat
    12061 actgcggatt gatttgaatg catgattgga gaataatttc caggggagcc gtgcctgcac
    12121 ttatttattt tattgttaat cctcaaaaaa aatcttaatg cctggacccg gagctttttg
    12181 gtttggaaag gaagaaactg atgccgtgat tgaagtaatc aggagcggtt atctatttcg
    12241 ttatggaagc gaaaatgacc cgtctttcct tcataagaca gcgactttgg aaagagaatt
    12301 tgcaagatac agcaatgccg gttatgccct tgccacatcg tcgggaacgg cttccctgct
    12361 ggtttcactc ctggccgccg gtttagagcc gggagatgaa ataattgttc ctgcctatac
    12421 atttgttgca agctattcat cagcaatttt tgcagggctg gttccggtcc ttgctgaaat
    12481 agatgaaagc ctgacccttg atcctgtcga tattgagtcg cggataaccc cgcgtacaag
    12541 ggcgataatg cccgtacaca tgctgggtaa tccgtgcaat atggatgccg taatgaaaat
    12601 tgcggaaaag cacggattac ttgttattga agatgcatgc cagtcggcgg gcgcctccta
    12661 caagggtaaa aaggccggaa cgatcggcaa tatcggggct ttttcactga atatattcaa
    12721 aacgatcaac agcggggacg gaggtattgt tataaccagc gacaaagatc tgtatgaaag
    12781 ggcatttgca attcatgatc agggacataa accgatgaga ttcggagtgg aggtcggttc
    12841 acgcaatatc cttggactga acttcaggat gaatgaactg acagctgccg tcagtcttgc
    12901 acagcttcag aagatagaca gaataatatc cacgctcagg gagaaacgag agaaattcaa
    12961 aaaactcatt tccccggcaa aaggattcag attcagaagg ctcaatgatc cagacggcga
    13021 ctgtgcaaca ctgtgcagcg tgatattcga cacaagggaa caggcggcca gggttgccgg
    13081 atcactggga tccaaaacag tcgatcagag cggctggcat gtttactcaa atatggaaca
    13141 tgtgctgagc catctgaaga aaatcggaca gccacatacc aggggtgcct atccgcggac
    13201 cgatgatatt ctgggcagga ctattaatat aagcgtggga gttgttgacg gggggctggg
    13261 ttccggatgg ggcataaaca taaattcaac ggatgaccag atagaagctg ctgcagagca
    13321 gtttatcgct gcctgtgaaa actgaatcca tcaccttttc tggtttacgc tggaaataat
    13381 caatgccgga ctgttattgc ccgtatattc ccggattatt atatttgttt ccgtcaaatt
    13441 ccggtattgt tatttaatta acgcataaaa cagggttaca ataaaaaaat tatttctcat
    13501 ggcaacacgc agggtatttg ttaaaaaagc ctcactgggc gctgcatcga ttgttttcag
    13561 tgcaaagagc tattccagga tcgtcggggc aaacgaccgg gtcagggtcg ggatcattgg
    13621 ttattccgac cgtttcaaag gctcattgtc tccttctttt ctcagcctgt caaaggaaat
    13681 gaactttgaa tttaccgtca tttccgatat ctggaggctt cgcagggagg aggcagacag
    13741 attttacaaa tcaatgggta tcaatctgcg gctggcacga aacaatgatg agctgtatga
    13801 aaagaatgac actgatgcag tcataataag cacagctgat tttcagcatg ctctccactg
    13861 tgccgaagcc gtcggggcgg gacgcgatgc atatgttgag aagccttttg ccgaaacgat
    13921 ggcagacaac cgtacagcac tgaaggctgt ccagaactcg gggaagattg tgcagatagg
    13981 ctcacagagg cggagtgcac ccaattatat cgctgcaaac gagtatataa aatcggggaa
    14041 attcggcaag attgtgatgg ttgaaatgac atggaatgtc aaccagccag ggagatggag
    14101 aaggccaaat gtggtcccga cactgaagga ggctgatacc gactggaaaa gatatctcat
    14161 gaatcgtcct ttaacagctt ttgatccaag gagatatctc gagttcaggc ttttctggcc
    14221 ttattcatca ggtattcccg gccagtggat ggcacatcag attgataccg ttcactggtt
    14281 caccgggctg gggcatccca ggagcgtggc tgcaaacggt ggtatttacc tgtggaaaga
    14341 cggccgcact aattttgaca caatgacggc tgtttttgat tacgggaata atgccgaccc
    14401 caattcaggc ttccaggtag tatattcgtc gagattcacc aattctgcag gcggagtcaa
    14461 ggagctatat ttttcaaacg gcgggatgct gaacctcgat acaaacagga tcacatcaga
    14521 aggaggactt gaagaatctg atgcaagtgc aatgaatatg aagccgaatc ttcttaaaac
    14581 atttgaactg ccttctgtat caattgagac agcagctgat accgggggcg ataccatgac
    14641 aattgcacat atgaaaaact ggatggaatg tgtcagaagc cgtaaaaagc cgaatgctga
    14701 cgttacggca ggatataatc attccattgc gaacatcatg tgcactgcaa gccttcgcac
    14761 gggagaaaag gcaactttcg acgagaaaaa ccaggaggta ctggctggtg gcaaaatttt
    14821 tcagtattga caaggcgggt aaacaaaaac atgtgtaaat gaaaagatca gttttatcag
    14881 tggcatttgc ggtattggca gccggaacgt tattgtattc atgcagatca tctgctactc
    14941 ccgatgaaaa acaaatgtat tatgaactga gaatttacag gttaaaggat cctgggaaga
    15001 atgatctcgt tgacaggtat ttgaaaaatg cttttattcc agccatgcgt aaggccggaa
    15061 tacaggcagt tggagtattc agacccgtgg aaacggatac agcttacggg aagatggtat
    15121 atgtcttcat tccatataaa aatatggatc agtattccgg ggcattggaa atacttgaaa
    15181 aagatcccga ccatcagaag gcgggcaggg aatttttaga tgcaccttat aatgatcctg
    15241 catttatacg gtacgaaagc atattcatga aagcttttgc ttttatgccg gtataccgta
    15301 aatatacctt cgacacccct ccggaagaaa gaatatatga actgaggagc tatgaaagct
    15361 ggacggaggt caaggcaatg aagaagatcc aaatgttcaa cgaaggaggc gagattgcca
    15421 tctttgacga gataggttca aatgccgtat tttacggaca ggttcttttt ggcagcctca
    15481 agccgcggct gatgtacctg acaacctatt caaatatgca atcgagggat gaacattgga
    15541 atgctttcag ggctcatccc gtgtggaaga agatctcagt tatggaggaa tatgccaata
    15601 cggtcatcag accggacgcc taccttctcc atccggcacc ttattccgac ttttgacgaa
    15661 agccggattc ggaatgaaaa ttaaccgcaa agctgccagt accggctcat tgcatacaaa
    15721 atcctgatat ccgtaatcag tctttgatga acatggccgg taacacagcc ggctttgcgg
    15781 ttattggttt tccggtcctg aataatcacg gattaaactg cgttcagtcg gggagctcaa
    15841 tttttgaaat tgtagcttca acttcggcag aggtcatttc atgatcgggg agatttcctt
    15901 cgaagtattt ctcgtaagaa gaaatatcga aatgaccatg gccgctaaga ttaaacagga
    15961 tcgtttttga gattccctca agatctgcct ttctggcttc atcgagtgca cctgcaatgg
    16021 cataatttga ttcgggagcc ggaaggatac cttcggttct ggcaaaaagc actccgccct
    16081 caaaacactc tctctgcatt tttgccttgg cttcgatgaa tccgtccttg agcagctggc
    16141 taaccagtac ccctgcacca tggtaccgca atcctcctgc atgtattttt tcaggaacga
    16201 agttatgtcc aagggtgaac attggcaaca ggggggtcat tcctgccgtg tcgccgaagt
    16261 catacatgaa ttttcccttt gtaagcttag ggcatgatga aggttccacc gtaacgaacc
    16321 tggtcttccg gtcatttaca atattttccc gtataaatgg aaatgcaatt cccgagaaat
    16381 ttgatccgcc tccgaaacat ccaatcacta cgtcggggta atccccggct ttttccatct
    16441 gcaaaagtgc ttcctgtccg attattgtct ggtgaaggca gacgtgattc agcacactgc
    16501 caagtgaata cttggtatag ggatcctgga cggcgatctc cattgcttcg gaaattgcaa
    16561 tgcccaggct tcccggtgaa tcgggatcct tttcaagaat ctttctgccc gcttccgtca
    16621 ttgtactggg cgaaggcacc acttttgcat tgtaagagtt catgagaagc tttctgccgg
    16681 gtttctgggt atagctgacc ctgaccatga aaaccagcag ttcaagtccg aaatgattgc
    16741 atgcgtaact cagtgcactt ccccattgcc cggcacctgt ttcggtggtg atcctcttta
    16801 tcccttcctt tttgttgtaa taggcctgtg caatggcag
//
LOCUS       DAOOYG010000054        16694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3374_length_16694_cov_6.646974, whole genome shotgun sequence.
ACCESSION   DAOOYG010000054 DAOOYG010000000
VERSION     DAOOYG010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16694
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3374_length_16694_cov_6.646974"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PK288_06340"
     CDS             complement(<1..722)
                     /locus_tag="PK288_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18575.1"
                     /translation="MEVTERLTYRRIFLFWGPLALTWLMMAIEQPFLIAFIARLDDAK
                     FNLAAFGIAGSFATIIESPIIMLMSASTALVTGRHSYIKLKKFTDILNAAITGIQLVI
                     LIPPVFRFIVIDLMEVPGNVAELAHVALLIFIPWAASIGYRRFYQGILIRNNLTRRVT
                     YGTLVRLSVIVIMGLILFFTGINGAYVGAGAMSLAVLCEAIATRMMVSRTLKDLMLKN
                     DTVNGNLSLRSVSKFYFPLALTS"
     gene            810..1352
                     /locus_tag="PK288_06345"
     CDS             810..1352
                     /locus_tag="PK288_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18576.1"
                     /translation="MSLLNPGAIREKVVNSSLSEQNIFFFVTIAATFIVKMIKKTAAS
                     AFLILAGLLLLVQAAVPHHHHGKLVCFAKSHCINDEPTDDRGNSHPDHSNDGDCGSDN
                     CMLKEPALISSNQPDSGFKFLNKNSCQDWNDNSPAGIPGSETDNQLSALLHSVIQTKI
                     SFPDPGLSFTSKGLRSPPAI"
     gene            1447..2949
                     /locus_tag="PK288_06350"
     CDS             1447..2949
                     /locus_tag="PK288_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM18577.1"
                     /translation="MNLKYFPVPLMILLISSCNPDHGTEHHDHHEETKIQFTAYNQNH
                     ELFAEADPFITGHTSKIMTYLSSLSDFKPVSASAVTLKMTVSGQTREQTIENPAQKGK
                     FSFEVKPEKSGNGTLEFIVKTDTGNEIIIVPEIKVYSSDHEADEDPEVHSHTAANTTV
                     FTKEQSWKIDFSTGYPLRESFGQAIKTTARVQPALNDEMLVTAKTNGIVSFSGNIILE
                     GTKLSEGQPLLMIKGSGFAENNSAVRFTEAQNNYEQAKADFDRMKLLASGKIVSDRQL
                     METKTQYENARTVYENLRQNFSLTGQTVNSPIAGFVRQLLVENGQYVEAGQPLILISQ
                     NRSLLLRAEIQQKYAGYLSSVTTAIIRKVEDNKTFTLEELKGKILSVGKAATNDNYLI
                     PLTIQVNNPGSLIPGSFVELYLKTVSDAEVLTIPNQAIIEEQGRYSIFVQVTPELFEK
                     REIKTGQTDGIRTEVISGLADNERIVTKGAVMIRLAQSTGALDAHSGHVH"
     gene            2964..6065
                     /locus_tag="PK288_06355"
     CDS             2964..6065
                     /locus_tag="PK288_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM18578.1"
                     /translation="MINNIIKFSLKNKYLVILLSLALVVFGIRTAINMDIDVFPDLTA
                     PTVVVMTDAHGMAPEEVERLVTFPIETSVNGATGVRRVRSASSHGYSFVWVEFDWGTD
                     IFRARQIVSEKLITVSSQMPLGVDQPVLAPQSSVMGEIFFIGLQADSTSLMDLRTIAE
                     WNIKPLLLATGGVSQVTIIGGDYKQYQVLANPQKMNFYNVSMNELSEACESISENSTG
                     GVVRQYGNEYVVRGIARASDTEILGNTLVSTFNGKPVRLNDVAEVKIGSAVKMGHASR
                     NASPAVIISISKQPHTNTLRVTEKIEENLSALKKTLPPDVKLDTKIFRQADFIETSVR
                     NVQNALIEGAVFVIIILFIFLGSFRTTIISLLAIPISLLGAIVAMKFLGLNINTMSLG
                     GMAIAIGALVDDAIIDVENVYKRLRENRLKPEGERQGSFTVVYEASKEIRASILNATV
                     IIIVAFLPLFFLSGMEGKMLKPLGISFIVSLFVSLIVAMTLTPLLSELLLSDERYLAR
                     NEKDKWLVRKLSHYYKRSLRWSLNHKKTILLSTLGLFIAVLILMSTRGRSFLPEFNEG
                     SLTISIVTRPGTSLEESNMLGNLVETELLAIPGISSTARRSGRGELDEHSQTTNSAEI
                     DVNFSLNGRDRDKLMAEVRNTLSQIPGIAFTVGQPLGHRIDHMLSGTRANIAIKLFGT
                     DLNRMFSLGKEVKGSIEGIGGLVDVNVEQQVEIPQIQIRANRDMLSWYGITLQDFNQF
                     VDLAFAGKKLADIYEDQRSFDLILRLDKDYTDNIEGIRNALIDTHDGKKVPLEQVADI
                     VSASGPSSISRENVQRKLVVSANVAGRDLRGAVQEIREKINTAIVLPEGYRIEYGGQF
                     ESEARASQTLLITSVFALLIIFLLLFQEFRNFRLAGIILLNLPLALIGGVMAIFLTSD
                     VISIPSIIGFITLFGIATRNGILLVSHYQRLQDIGTPLGETIIKGSADRLNPILMTAL
                     TAALALIPLALRGSLAGNEIQSPMAKVILGGLITSTLLNIYIVPMVYSIFRNHGIISN
                     NDL"
     gene            6270..6629
                     /locus_tag="PK288_06360"
     CDS             6270..6629
                     /locus_tag="PK288_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HPM18579.1"
                     /translation="MTKKILILINDAPYGSEKAYNAFRLAMQLCREHDDVEVRIFLMA
                     DAAACAVANQNTPAGYYNIERMLKAVLAKGGMVKICGSCIEARGLKNTMLTEGTEIST
                     MAALANWVIDSDKVITF"
     gene            6693..7883
                     /locus_tag="PK288_06365"
     CDS             6693..7883
                     /locus_tag="PK288_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM18580.1"
                     /translation="MIYIFKELRRHKWRTIFSVTGYALATVFILLILSVADANKNDSY
                     GVLQSTGTHFIVYIPTEANLCTSNIANGSVFAEGVKTMMLDHDMVRTIRNVEGVKDAA
                     ACLQYKMFDGVFQSEVSIAGIDTSSLATRTNVLAPAHVISGRYFSGRPEELLAEESFA
                     AAHDLSPGDTLKIFGGKMIVAGIVNSGIRPVKADFYAPISLVRTILKDRLLCNAPNFD
                     MNIILVEVADARVQDDVITRIRNMMYKFAVSTYNCYQPANRVMSIIDRSSIGLTGLIF
                     LFLVIFSAKTQLTALMERFREIGILKSLGWSDSSLAIHVFLVSLIQSVAGVTAGVLLA
                     FGLIEFLGHLDNPVFSSYSFRLRFGTIPLIYALSLIGAVAAGLYPAIRIFRTKAGDII
                     RNHM"
     gene            7903..8583
                     /locus_tag="PK288_06370"
     CDS             7903..8583
                     /locus_tag="PK288_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM18581.1"
                     /translation="MNNYKIECISLNKQYKGRNVLITAVRDVSLKIEKGEFILLKGRS
                     GAGKSTLINLIGGLIAPTSGKVTIDGFIVNQLNNKSLSNLLLNRVGIVFQGFNLLPTY
                     DIYENIEVALAPRGLSKREIKKLAIPYFERFGLSEMMHLLPEELSTGQQQKVAVIRTL
                     IRQPSIILADEPTASIDDITAGEILELMRNLCNEQNVTIILATHGIVPETIADRMIVM
                     ENGKVINQ"
     gene            complement(8591..9736)
                     /locus_tag="PK288_06375"
     CDS             complement(8591..9736)
                     /locus_tag="PK288_06375"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPM18582.1"
                     /translation="MKISPRFLMMTLLISLILNPSLCRERIKKNQEEYLFESGTNGYT
                     TFRIPAIITTIKGTLLAFAEGRKNSSSDTGDIDLVMRRSDDNGKTWSDLIVIWDDKDN
                     VCGNPAPVQDVVTGKIFLLSTWNLGSDNEPDIIKGKSMDTRRIFLLISSDDGRTWSEP
                     SEITGSVKQTNWTWYATGPCHGIQVREGKYRNRLIIPCDHIESVTERYYSHVIYSDDN
                     GSTWHLGGTTPQDQVNECSVAELADGRLMLNMRNYDRIRKTRKVSVSTDGGISWSDIY
                     PDPFLVEPVCQGSLLAHNKKKILLFSNPADEISRVRMTLRVSSDQGKTWAHSRVLHEG
                     PAAYSDITILRSGRVGCLFEMGHKSPYEGIVFRSLKLKDLMAGNQVD"
     gene            10254..12149
                     /gene="acs"
                     /locus_tag="PK288_06380"
     CDS             10254..12149
                     /gene="acs"
                     /locus_tag="PK288_06380"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPM18583.1"
                     /translation="MNNLNDRRFYYPSQQITENAIVKDYDELYRYSVEHREEFWAEQA
                     ENLFWHKRWDAVLDKSNPPFYRWFPGARTNIVYNALDRHQQTPVRNKLALIWEGEPGD
                     VRTFSYHSVNREVSQFANVLKSMGVKKGDVVTIYMPQIPEQIFAMLACARIGAVHSVV
                     FGGFSHESLADRINDANSRVVITADGGYKRGKVVEMKSIVNKAMELSPTMEICITIRR
                     ANVDVYMENGRDYWYHDLVGLPIASYKCETEVMESDDMLFLLYTSGSTGKPKALVHTH
                     GGYSVYTSTTHRMVFDLKPEDRYWCAADPGWITGHSYIVYGTLINGATVVMYEGAPTY
                     PYPDRWWKMVDKYGVNILYTSPTAIRSLMRFGDSWPKRNDLSSLRLLGSVGEPINPEA
                     WKWYHEIIGNGRCPIMDTWWQTETGGFMITPLPITPLKPGSASKPFFGNEISVVNEEG
                     REVNVGEEGNLVIKSPWPGMTAGVYKDNQRFIDTYWSKYSKQGWYQTGDSAKIDEDGY
                     FWIIGRTDDVIKISGYRLGSYELESIMSSHAAIVESAAIALPHPVKGNCIHIYAVLRK
                     GYEPGKELEKELIGHVITHLGPIAKPEEIIFVDQLPKTRSGKIMRRVLKARALGLPEG
                     DLSTLED"
     gene            complement(12187..12414)
                     /locus_tag="PK288_06385"
     CDS             complement(12187..12414)
                     /locus_tag="PK288_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18584.1"
                     /translation="MKRPILLILITFILGITFTGCMTGRKYQADLRGLMLQDNLQMKR
                     NRAFYSKHNAKLRKQAFRHSRRSGRIFGSKY"
     gene            12576..12767
                     /locus_tag="PK288_06390"
     CDS             12576..12767
                     /locus_tag="PK288_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18585.1"
                     /translation="MNNKRSNKPRNTKFVRIDNSTWIETDALVPDDVARMQFLQKVQL
                     SRPGSFESQVKDDLFYLRT"
     gene            complement(12867..13874)
                     /locus_tag="PK288_06395"
     CDS             complement(12867..13874)
                     /locus_tag="PK288_06395"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HPM18586.1"
                     /translation="MKTKELLQYIGNLDQLGGTRHYELTDGRGRNMRAIDVNSGSGLN
                     YTVLPDRGMDISLASYRGINLVYLTSTGESHPAFYEPENSGWLRTFTGGLLTTCGLTY
                     LGPPVLDEDELLGLHGRYSNIPARQVADLSHWDEDEYHIRLRGIVEEAALFGNKIRLE
                     REITTTIGQNRIRIADAITNFGYAESPYTILYHMNFGYPFLSEESELIINPEETIPRD
                     EHSASGAVNFKRFSLPQAGFTEQVYFHRLRPDEKGISSVSLINKKSGIDLTIEFTGAA
                     LPWFTQWKMMGPGEYVLGLEPGNVPVRNRKVLREEGLLPFLQPGETVTNQVDVTVKEL
                     H"
     gene            complement(14018..15187)
                     /locus_tag="PK288_06400"
     CDS             complement(14018..15187)
                     /locus_tag="PK288_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPM18587.1"
                     /translation="MKLKLLLFACFFGFISAHSQESFLCQGYYTTEEGGRANLEKFSR
                     TYHDQASWMKRAGQIRATILKGAKLDELPARCNLNPIRKIKQVFDGYTVENVAFESLP
                     GFYVTGNLYLPASFTGKIPGIACPHGHWNNPADYGRYRIDMQYRCAALALMGAAVFAY
                     DMVGYGESLPCLHGTPEVLRLQTWNSMRVIDFLLSLGFVDDTRLGVTGASGGGTQSFL
                     LAAVDDRIDVSVPAVQVSAHFYGGCNCESGMPIHRTPGFETNNVEIAASFAPKPQLII
                     SDGDDWTRNVPDIEYPYIKKVYSLFGKSENVENAHFENEIHDYGYNKRVAMYRFMAKH
                     LGLKLENLTDENGKINDSNAQLQILPLLKVFPDGVRPENEVKSCDEVIRLLNTYR"
     gene            complement(15515..>16694)
                     /locus_tag="PK288_06405"
     CDS             complement(15515..>16694)
                     /locus_tag="PK288_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18588.1"
                     /translation="VARVQQLIDIAVNFRDSRNVNIFCGEFGVYIPNSGNTDRSYWYN
                     AVRQYLDEKNIPWTIWDYQGGFGLFNKNSNELFDHDLNITLLQSLGLNIPPQTPFVIT
                     PETNGFLIYSDFICDQISDGSYGTGKINFYSTDLPNNGIYNLLWQDFGQYNAIVFRFK
                     PFKDLSKLAAGGYAVDFMVRGNKPDIKFDIRFVDTKTDEQDHPWRMGITIDGTVAEWD
                     RKWHHVHIPLSDLYEKGSWDNNSWYDPEGKFDWSAVERFEISSEFPVSSGQYVWFDNI
                     HVTNLDTAQVREDRVVNVSEVSEQALPSLMIAPNPVRGHATVAYELAGEDHVDLSIFS
                     LTGLKILTLDKGVRGPGLTILTWNGCIDPGKRIGQGMYFCVLTTSRFKVSCKLLVAGN
                     "
BASE COUNT         4829 a         3761 c         3659 g         4445 t
ORIGIN      
        1 gaagtcagcg caagggggaa atagaacttt gaaaccgatc tcaggctgag attaccgttt
       61 accgtatcgt tcttaagcat cagatcttta agggtcctgg aaaccatcat ccttgtggct
      121 atagcttcgc aaagtacggc cagcgacatt gcccccgcac caacgtatgc cccgttgatt
      181 cccgtaaaaa acaatatcaa acccataatt acaattaccg ataaccttac cagggtacca
      241 tatgtcaccc gtcgtgtcag attattcctg ataaggatac cctggtaaaa tctcctgtaa
      301 ccaattgagg cggcccaggg aataaagatc aggagagcga catgggcgag ttctgccacg
      361 ttccccggaa cttccatcag atcaatgaca atgaacctga agaccggagg gataaggatg
      421 acaagctgaa taccggtaat ggccgcatta agtatatcgg tgaatttttt cagtttaatg
      481 taggaatggc gtccggttac cagagcggtg gatgcactca tcagcatgat aatcggcgac
      541 tcaatgatgg ttgcaaaaga tccggcaatg ccaaaggcgg caagattgaa cttggcatca
      601 tcaagccttg cgataaaggc aataagaaaa ggctgctcga tggccatcat aagccaggta
      661 agggccagcg gcccccagaa aagaaatatc ctccggtatg tcagcctttc agttacctcc
      721 acactatcaa aatcgcaaag atagacaatt cactgcgatg gagatctttt tcatcttttc
      781 actgatcagc ccggactcaa tcagtattaa tgtcattatt aaatcccggg gcaatacgtg
      841 aaaaagttgt taatagttca ttgtctgaac aaaatatatt ttttttcgtt actattgcag
      901 ctacttttat cgtaaaaatg attaagaaaa cagcagcaag tgctttttta attcttgccg
      961 gtttattact gctggttcag gctgctgtgc cgcatcatca tcacggtaaa ctggtctgtt
     1021 ttgcaaaatc ccattgcatc aatgatgaac caacagatga caggggaaat agccatcccg
     1081 atcacagtaa tgacggtgat tgcggttctg acaattgtat gttgaaagaa cctgccctga
     1141 tcagctcaaa tcagccggat tcagggttta agtttttaaa taaaaactcc tgccaggact
     1201 ggaatgataa ttctccggca ggtatcccgg gcagtgaaac ggacaatcag ctatctgctt
     1261 tactgcattc agttatacaa acaaagataa gtttccctga ccccggcctt tcttttacct
     1321 ccaaaggtct gcgctccccg ccagcaatct gatcttccgt actgatttat atgacagcat
     1381 tccgcaggaa tttaatgttc agcgggatct atccattatt tattctattc ccaaacagtc
     1441 attcacatga atttaaaata ttttccggtt ccgctgatga tactgctgat ctcatcatgt
     1501 aatcccgatc atggtacgga acaccatgac catcatgaag agacaaagat tcagtttaca
     1561 gcatacaatc aaaaccatga gctgtttgct gaggcagatc cattcattac agggcatacc
     1621 tcaaaaataa tgacttatct atccagcctc tctgatttca aaccagtgtc cgcgtctgca
     1681 gtaaccctga aaatgactgt cagcgggcaa acccgcgagc aaacaattga aaaccctgcg
     1741 caaaaaggaa aattcagctt tgaagtcaaa cctgaaaaga gcgggaacgg cacattggaa
     1801 ttcattgtca aaaccgacac tggtaatgaa ataatcattg ttcctgaaat aaaagtatat
     1861 tccagtgacc atgaggcaga tgaggatccc gaagtacatt cacacactgc agcaaatacc
     1921 acggttttta caaaagagca atcctggaaa attgattttt caaccggcta ccctttgagg
     1981 gagtcattcg gacaggcaat aaagacgaca gcccgggttc agcctgcatt gaatgatgag
     2041 atgctggtga ctgcaaaaac aaacggtatt gtatcattct caggaaacat catcctggaa
     2101 ggaacaaaat tatcagaggg tcagcctctc cttatgatta aaggcagcgg tttcgccgaa
     2161 aacaattctg ccgtccggtt tacggaagca cagaacaatt acgaacaggc aaaggcagat
     2221 tttgacagga tgaaacttct tgcaagcggg aaaattgttt ctgacaggca attaatggaa
     2281 acgaagaccc agtatgagaa cgccaggact gtgtatgaaa atctcagaca gaatttcagc
     2341 ctgacagggc agacggtcaa cagtcctata gccggatttg tcagacaact gcttgtggaa
     2401 aacggacaat atgttgaagc tggccagcct ctgatactga tctctcaaaa caggtcattg
     2461 ctccttcggg ctgaaatcca gcagaaatat gccggatatc tcagttcagt aacaacagca
     2521 atcataagga aggttgagga taacaaaacc ttcacccttg aagaacttaa gggaaagatc
     2581 ctgtccgtcg ggaaagctgc caccaatgat aattatctta ttcccctgac cattcaggtg
     2641 aataacccgg gtagccttat ccccggaagt tttgtggaac tgtatcttaa aaccgtatcg
     2701 gatgccgagg ttctgacaat accaaatcag gcaataattg aagagcaggg caggtattcc
     2761 atatttgtcc aggtcactcc tgaactgttt gaaaaacgtg aaataaagac aggtcagaca
     2821 gatggcataa gaacggaagt tatcagcggt cttgctgata atgaacgtat tgtaaccaag
     2881 ggagctgtca tgataaggct tgcacaaagc accggggccc tcgatgcaca ttcaggtcat
     2941 gtccattaat aacgcggaaa tccatgatca ataatatcat aaagttctcc ctgaagaata
     3001 agtatcttgt tatactgctt tcattggcgc tggtggtttt cgggatcagg acagccataa
     3061 acatggatat cgatgtattt cccgatctga ctgctccaac cgtggttgtc atgaccgatg
     3121 ctcacgggat ggccccggaa gaggttgaac ggcttgtaac atttcccatt gaaacttccg
     3181 tgaatggtgc cacaggtgtg cgaagggtca ggtcggcctc atcacacggc tactcttttg
     3241 tatgggtaga atttgactgg ggtaccgaca ttttcagggc gcggcagata gtaagcgaaa
     3301 agctgatcac ggtctcctca cagatgcctt tgggagttga ccagccggtg cttgctccac
     3361 aatcaagcgt tatgggggaa atatttttta tcggactaca ggcggacagc acaagtctga
     3421 tggatctgag aaccatagca gaatggaaca ttaaaccatt gctgctggcg acgggcggtg
     3481 tctcacaggt gacaattatt ggcggtgact ataaacaata ccaggtgctt gccaatccac
     3541 agaagatgaa tttctacaat gtctcaatga atgaattgtc tgaggcttgt gaaagcatca
     3601 gtgaaaactc aaccggaggt gtggtcaggc agtacggtaa tgagtatgtg gtcagaggca
     3661 ttgcccgggc ctcagatacc gagatcctgg gtaacactct tgtcagtacc tttaatggca
     3721 aaccggtcag gctgaacgat gttgctgaag ttaagatcgg aagcgcggta aagatgggac
     3781 atgcttcaag aaacgccagc ccggccgtga ttatatccat atccaagcaa cctcacacaa
     3841 acacactcag ggttacggag aaaatagaag aaaatctaag tgccttgaag aaaacattac
     3901 cgccagatgt taaacttgac acaaaaattt tccgccaggc cgatttcatt gaaacctccg
     3961 tacgcaatgt tcagaatgca ctgattgaag gggcagtatt tgtcattatc atacttttta
     4021 tatttcttgg aagtttcaga acaacgatca tttcgctgct ggcaattcct atctccctgc
     4081 ttggtgcaat tgttgccatg aaatttctcg gattgaatat caatacaatg agtcttggcg
     4141 gaatggccat agcgattggt gcgctcgttg atgatgccat aattgatgtt gaaaatgttt
     4201 acaagcgtct tcgcgaaaac cgactgaagc ctgaaggaga gcgtcagggt tccttcacag
     4261 tggtgtatga agcatcaaaa gagataaggg cctcaattct gaatgccacg gtcattatca
     4321 ttgtggcatt cctcccattg ttttttctat cgggaatgga agggaaaatg ctgaagccgc
     4381 tgggcatatc ttttatcgtt tccctcttcg tgtcacttat tgtggcaatg acactgactc
     4441 cgctgttatc cgaactgcta ttatctgacg agaggtacct tgcaaggaat gaaaaagata
     4501 aatggcttgt gcgtaagctt tcgcattatt ataaaagatc tctcagatgg tcgcttaacc
     4561 ataaaaaaac aatcctgtta tcaacactcg gcttgttcat tgctgtcctg atcctgatgt
     4621 caacacgggg tagaagcttc cttcctgaat tcaatgaagg gtcattgacc atttccatag
     4681 tcaccaggcc cggtacctcc ctggaagaaa gcaacatgct gggcaacctt gttgaaacag
     4741 aattgctggc aattccaggc atatccagca cagcacgcag atcagggcgg ggtgagcttg
     4801 atgaacactc acagacaacc aacagcgctg aaatcgatgt taatttctca ctcaacggac
     4861 gggatcgtga taaactcatg gccgaagtcc gaaataccct gtcgcagatt ccgggaatag
     4921 catttactgt gggacagcct ctcggtcatc gcattgacca tatgctctcg ggaacacgtg
     4981 ccaacatcgc aataaaactg tttggaaccg acctcaacag gatgttctca ctcgggaagg
     5041 aggtaaaagg ttcgattgag ggcattggag gccttgttga cgttaatgtt gaacagcagg
     5101 ttgaaatccc gcagattcag atacgggcaa accgggatat gctttcctgg tatgggatca
     5161 cgctgcagga cttcaaccag ttcgtcgacc ttgctttcgc tggaaaaaaa ctggcagata
     5221 tttatgagga ccagcgcagc tttgatctca ttttaaggtt ggataaggat tatactgata
     5281 atattgaagg tatccgcaat gcgcttatag acactcacga cgggaagaag gtccccctcg
     5341 aacaggttgc agatattgtc tccgcttcag ggccaagttc aatcagccgt gagaatgttc
     5401 aacgaaagct ggttgtttct gcaaatgtgg caggacgtga tctgcggggg gcagtccagg
     5461 aaatacggga gaaaataaat actgcaatcg ttctgcccga gggatacagg attgaatatg
     5521 gcggccagtt tgaaagtgag gccagggcat cacaaactct cctgataacc tccgtatttg
     5581 ccttgctgat tatattcctg ctgttgttcc aggaattcag gaacttcagg ctggccggaa
     5641 ttatcctgct taacctgcca ctggccctga tcggaggtgt tatggcaata ttccttacat
     5701 cagatgttat aagcatcccc tcaatcatcg gatttattac cctgtttggc atagccacaa
     5761 gaaacgggat acttctcgtt tcccattacc agcgcttgca ggacattggc acaccgctgg
     5821 gtgaaacaat cattaaaggt tctgccgaca ggcttaaccc gatactgatg acggccctga
     5881 ctgctgccct tgcgcttatc ccgctggcac tgaggggcag tcttgcagga aacgaaattc
     5941 aaagtccgat ggcaaaagtc atactgggag gtttgataac ctcaacttta ttgaacatct
     6001 atattgttcc aatggtgtac agcatattca gaaatcacgg aataatatcg aacaatgatc
     6061 tttaaagcag gcaaccgcag gccggaatta agggtcatct gcctgaaagc agaaccatac
     6121 gtcactgtcg gtcggatact atgggacggg gtcagcagct gcttctgaaa cccgcattga
     6181 cattcatctc tttttcataa ttcctttaat gattataaag gctttttatg atatattcac
     6241 gagattaaaa cttgtgaaac catattctta tgacgaagaa aatcctcatt ttgataaatg
     6301 atgctcccta tggttcagag aaggcataca atgctttccg gctggcaatg cagctttgca
     6361 gggaacatga tgatgttgag gtaagaatat ttcttatggc agatgcagct gcctgcgcag
     6421 ttgcaaatca aaataccccg gccggatatt acaatatcga aaggatgctt aaggctgtcc
     6481 ttgcaaaagg aggaatggtt aaaatatgcg gcagttgcat tgaagccagg ggcctgaaga
     6541 acacaatgct gacagaagga actgaaataa gcacaatggc cgccctggca aactgggtta
     6601 ttgacagtga caaggtgatt acattctgat atttgttcag aatcctttta acataaccgg
     6661 aacaaaaatg acccgttaaa aaatcattca gaatgatata tatattcaag gaacttcgca
     6721 ggcataaatg gagaacaata ttcagtgtta ccgggtatgc ccttgccacc gtgttcatac
     6781 ttctgatact ctcagtggca gacgcaaata aaaatgattc ttacggtgtc cttcagagta
     6841 ccggaaccca ttttattgtt tacataccta cagaagccaa tttatgtacc agcaacattg
     6901 ccaatggttc tgtttttgca gaaggtgtaa aaacgatgat gctggaccac gatatggtca
     6961 ggacaatcag gaatgttgaa ggtgtgaagg atgcggcagc atgtcttcag tacaaaatgt
     7021 tcgacggagt ttttcagtca gaagtttcaa ttgcgggtat cgacacctca agtctggcta
     7081 caagaacaaa tgttcttgcc ccggcacatg taatatcagg caggtacttt tccggcagac
     7141 ctgaagaact actggctgaa gaatcatttg ccgctgcaca tgatctgtcg ccgggggata
     7201 ccctgaagat attcggtgga aaaatgattg tggccggcat agtgaattcc ggtatacggc
     7261 ctgtgaaagc agatttctat gcacccatct cccttgtcag gacaatcctt aaagacaggc
     7321 tgctctgcaa cgcccccaat ttcgatatga atatcattct ggttgaagtt gcggatgccc
     7381 gtgtacagga tgatgtgatc accaggatca ggaacatgat gtacaaattc gcagtatcaa
     7441 cctataactg ctatcagcca gccaacaggg taatgtcgat aattgacaga tcatccattg
     7501 gcctcacagg actgatattt cttttcctgg taattttttc ggcaaagact cagctcactg
     7561 ccctgatgga aagattcagg gaaataggaa tcctgaagtc attaggctgg tcggattcca
     7621 gcctggccat acatgttttc ctggtatccc ttatacagtc agttgccgga gtaacagcag
     7681 gagtattgct cgcattcggt ctaattgaat tcctgggaca tcttgataat ccggttttca
     7741 gttcttacag tttccggctc cgcttcggaa ctatcccgtt aatatacgct ctctcactga
     7801 ttggtgccgt tgccgcaggt ctttatccgg caataaggat attccggaca aaggccgggg
     7861 atattatccg gaatcacatg taatcttatc ggaattggaa ttatgaacaa ctataaaatt
     7921 gaatgcatct ccctgaacaa acaatataaa ggcaggaatg tacttatcac tgccgtcagg
     7981 gatgtcagcc tcaaaatcga aaaaggcgaa ttcatattgt tgaaagggag gtcaggagca
     8041 ggaaaatcta ccctgataaa cctcatagga ggactgatcg caccgacgtc aggtaaggta
     8101 actattgatg gcttcattgt aaatcaactt aacaataaat cattgagcaa cctgttgctg
     8161 aaccgggttg ggattgtttt ccagggcttc aatctccttc ccacatatga tatttatgag
     8221 aacatcgaag ttgccctggc ccccagagga ctttcaaaaa gagaaataaa aaaacttgca
     8281 ataccatatt ttgaacgttt cggactgtca gaaatgatgc atcttctacc cgaagaactc
     8341 agtacgggac aacagcagaa ggtggcagtc atccggaccc tgatacggca gccatccatt
     8401 attctggccg atgagccgac tgcctcgata gacgatatta cagccggcga gatactggag
     8461 ctcatgagga atctgtgcaa tgagcaaaat gtaacaatca tactggcaac tcacggtatt
     8521 gtccccgaaa cgatcgctga caggatgatt gtaatggaaa acgggaaagt gatcaatcaa
     8581 tgacatattt ttaatcaacc tgattacctg ccatcagatc cttcaacttc aggcttctga
     8641 acactattcc ttcataagga gacttatgcc ccatctcaaa aagacatcct accctgcctg
     8701 atcttaagat ggtaatgtct gagtaagctg caggtccttc atgaagtact ctcgaatgtg
     8761 cccaggtttt tccctggtca gaactgactc tcagggtcat tctcacccgt gaaatctcat
     8821 ctgcaggatt ggaaaacaac aggattttct ttttattatg tgccaggagg gagccctgac
     8881 acacaggttc aacaaggaaa ggatcaggat aaatatcgct ccagctgatt cccccgtcgg
     8941 tacttactga aacttttctt gttttccgga tgcgatcata attcctcata ttaagcataa
     9001 gcctgccgtc agccagctca gcaacggaac attcgttaac ctgatcctgg ggagtggttc
     9061 cgcccaaatg ccaggtggag ccattgtcgt cagagtaaat aacatgcgaa tagtatcttt
     9121 ctgttaccga ttctatatga tcgcatggaa ttataagcct gttcctgtat ttcccctccc
     9181 tgacctgaat tccatggcat ggtccggttg cataccatgt ccagttagtt tgcttaactg
     9241 atccggtgat ttccgaaggc tctgaccatg tcctgccgtc atcggatgag atcagcaaaa
     9301 atatccgtcg tgtatccata ctctttccct taataatatc cggttcatta tctgagccga
     9361 gattccaggt cgataaaaga aaaatctttc ccgttacaac gtcctgtacc ggagccggat
     9421 taccgcaaac attatcttta tcatcccaga taacaataag atcactccag gtctttccgt
     9481 tatcatccga ccgtctcata accagatcga tatcccccgt atcggaagag ctgttctttc
     9541 tgccctcggc aaaggccaga agagtgcctt taatggtagt aattattgcc ggaatcctga
     9601 aagtggtata cccgtttgtc ccgctctcaa ataaatattc ttcctgattt ttcttaatcc
     9661 tttcacggca taatgaagga ttgaggatca aagatatcag caaggtcatc atcaggaatc
     9721 tcggggaaat tttcatctgt aacatttatt gttttaaaaa tctgattgct gtcaaattct
     9781 gcccggatct aaaaataaac attattgttc ctcggtaaat taaaccccgg gactattttt
     9841 cggaaagcag gttttatcga tacaacaaac cgcctgcctg tttcggttgg aatcactctc
     9901 cctgttgtgt cggcttccag gcaaggcttg aatgatggct gtccagttta tggtcaggca
     9961 tatctggttt atccggaatt atgtaatggc ctgtcttacc aataacaggc attaacagta
    10021 tatggtacat cagggactgt gcccgggatc gatttgcagt atcgtcggtg atcacctcaa
    10081 caaaacattg tccggcaccg cgattaatag tgaaaaatat agtgggaatg gatcgacagg
    10141 gatcaacggt ccataaaaaa tcaacagaaa aatcttaacc gacaattgaa caatattctg
    10201 tagaacaatg ttatggtttt ggaataagtt gttgaactta atcttactca tctatgaata
    10261 acctgaatga ccggagattt tattatccct cccaacaaat aactgaaaat gccatagtta
    10321 aggactatga tgagctttac cggtactcag tggaacaccg tgaggagttc tgggcagaac
    10381 aggctgagaa tctcttctgg cacaaaagat gggatgccgt gctggataaa tccaatcccc
    10441 ccttttacag gtggtttccc ggagcaagga ccaatattgt ctacaatgcg cttgatcgtc
    10501 atcaacaaac gccggtaagg aacaaactgg cactgatatg ggagggagaa ccgggcgatg
    10561 tcaggacatt ctcctatcat tcggtaaacc gtgaagtttc gcagtttgca aatgtactga
    10621 aaagcatggg tgtaaaaaaa ggcgatgtgg ttacaatcta tatgccccag atccctgagc
    10681 agatttttgc gatgcttgcc tgtgcccgga ttggagcagt tcacagtgtg gtttttggcg
    10741 gattcagtca tgaatcactt gcagacagga taaatgatgc aaacagccgc gtggttatta
    10801 ctgccgacgg cggatacaag cgtggcaagg ttgtggaaat gaaatcgatt gtaaacaagg
    10861 caatggaatt gtctccgaca atggaaatat gtataacgat aagaagggca aatgttgatg
    10921 tttacatgga aaacggaagg gattactggt atcatgacct tgtcgggctg cccattgcat
    10981 cctataaatg cgaaacggaa gtaatggaat ctgacgacat gctttttctc ctttatacat
    11041 caggttccac aggcaagcca aaagcgcttg ttcacacaca tggcggatac agtgtctaca
    11101 cctctacaac ccaccgcatg gtgtttgact taaaaccgga ggacaggtat tggtgtgcag
    11161 ctgatccggg ttggattacc gggcacagct acattgtata cggcactctg ataaatggtg
    11221 caactgtggt catgtatgaa ggtgcaccta catatcccta tcctgacaga tggtggaaaa
    11281 tggttgacaa gtacggagtg aacatattat acacttcacc tactgcaata agaagcctga
    11341 tgcgtttcgg cgactcatgg ccaaagagaa atgatctgag ttccctgcgc cttttaggat
    11401 cagtgggtga acccatcaac ccggaagcat ggaaatggta tcatgaaatc atcggtaatg
    11461 gcaggtgccc gataatggat acctggtggc aaactgaaac aggcggattc atgataacac
    11521 ccctgccgat caccccgctc aaaccgggtt ccgcatcaaa accttttttc ggaaatgaaa
    11581 tttcagttgt aaacgaggaa ggccgggaag tgaatgttgg tgaagaagga aatctggtaa
    11641 taaaatcccc ctggcccggt atgacagccg gagtttataa ggacaatcaa cgttttattg
    11701 atacgtactg gtccaaatac agtaagcagg gttggtacca gacaggtgat tcggccaaaa
    11761 tagatgaaga tggttatttc tggataatag gaaggactga tgatgttata aaaataagcg
    11821 gataccggct tggttcatat gagctcgaaa gtattatgag cagccatgcc gcaatagtgg
    11881 aatcggccgc catagcccta ccccaccctg tcaaaggtaa ttgcattcac atctatgcag
    11941 tgttgaggaa aggatatgag ccgggcaaag aactggaaaa agaacttatt ggtcatgtga
    12001 tcacccacct ggggcctatt gcaaaacctg aagaaataat tttcgttgat cagcttccaa
    12061 aaacaagaag cggtaaaatc atgcgcaggg tattgaaggc tagggccctt gggcttcctg
    12121 aaggggacct cagtacactt gaagattaat atacccgcta acagactgac cgggaataag
    12181 tattggtcag tattttgaac cgaagatccg gccgcttctt cttgaatgcc tgaaagcctg
    12241 cttccttagc ttggcattgt gtttggaata gaaagccctg ttccttttca tctgcaggtt
    12301 atcctgaagc atcagccctc tcagatcagc ctgatatttc ctgccggtca tgcaacctgt
    12361 gaaagttatc cccagaatga atgttatcag tattaataat atcggccttt tcatttttca
    12421 gtactttgtc ggataaaaac catatttaaa acttatttcc tgtagattta gaagtacatt
    12481 ttcaatcttt aaacggattt gcaaaaaaaa agttgcattt tgcaatatac tcaggctaca
    12541 tggcgtttta acaacgaaac catgatacgc cggaaatgaa taataaaaga tcaaacaaac
    12601 cgagaaatac caaattcgtc aggatagata attccacctg gatcgaaaca gatgccctgg
    12661 ttcctgatga tgttgcaaga atgcaattcc tgcagaaggt tcagttgtcg agacccggtt
    12721 catttgaaag tcaggtcaaa gatgatttat tttacctgag aacctaatat tatccttccc
    12781 ttaatcgtaa aatcagggct gaatgattcg tgttgccggt ttatggccgc atcgtaaaaa
    12841 agcctcagat tattattgct gtaaaatcaa tgtaattcct taacagttac atcaacctga
    12901 ttggtaactg tttctcccgg ctgtagaaat ggcagcaggc cttcctccct caatactttt
    12961 ctgttcctga ccggaacatt ccccggttca agtccgagaa catactcacc gggacccatc
    13021 attttccact gagtaaacca tggcaatgca gcccctgtaa actcaatggt gaggtcaatc
    13081 cctgattttt tatttatcag cgaaactgag gaaatccctt tttcatccgg tctcaacctg
    13141 tgaaagtata cctgttctgt gaatccggcc tgcggcaggc tgaaacgctt aaaatttact
    13201 gctcctgatg cagaatgttc atcccttgga attgtttcct ccggattaat tatcagttcc
    13261 gattcttcag aaaggaacgg ataaccgaaa ttcatgtgat atagaattgt atacggactt
    13321 tctgcatatc cgaaattggt gatcgcgtca gcaatgcgaa tcctgttctg ccctatggta
    13381 gttgtaattt cccgttcaag ccggatcttg tttccaaaaa gagcggcctc ctcaacaatt
    13441 cccctcagcc tgatgtgata ttcatcctca tcccaatgtg acagatcagc cacctgacgg
    13501 gcaggaatat tggaataacg tccatgcaga cccagcaact catcttcatc caaaacagga
    13561 ggcccaagat aggtcaggcc gcaggtcgtc agcagtccgc ccgtgaaagt cctcaaccag
    13621 cctgaattct caggctcgta aaaagccgga tgagattccc ccgtgctggt gagataaaca
    13681 aggttaatac ccctgtaaga agcaagggaa atatccattc cccggtcagg aaggactgta
    13741 taattcagcc cgcttccgga gttgacatca attgcccgca tgttacggcc cctgccatca
    13801 gtaagctcgt aatgccttgt ccccccgagc tgatccagat ttccaatata ttgcaatagc
    13861 tcttttgttt tcatttcagg ttattttatg tgttcgcaga ctattaaccg cgatttaaaa
    13921 tgaattttca tcttattacc tgttgccggc ttcaggctcc cctgacatga tcagtactta
    13981 aaaccatttt cagggcggag atacttaaaa taggattcta tctgtaggta ttaagaagcc
    14041 ttataacctc atcacagctt tttacttcat tttcaggacg gaccccgtcg ggaaaaactt
    14101 taagcaatgg cagtatctgc aattgtgcat tactgtcatt gatctttcca ttttcgtctg
    14161 tgaggttttc aagcttcagt ccgagatgtt ttgccatgaa cctgtacatt gccactctct
    14221 tgttataacc ataatcatga atctcattct caaaatgggc attttcgaca ttttctgact
    14281 tgccgaacag gctgtaaacc tttttgatat acggatattc tatatccggg acattccttg
    14341 tccagtcatc tccatctgag attatcagct gtggctttgg tgcgaatgat gcagcaatct
    14401 caacattgtt ggtctcaaag cctggggtcc tgtgaatggg cataccgctt tcacaattac
    14461 atccgccgta aaaatgagct gatacctgca cggccggaac agagacatca attctgtcat
    14521 cgacagcggc aagcagaaag gattgggtcc cgcctccaga tgctccggta acaccaagtc
    14581 tcgtgtcatc aacaaaacca agtgaaagga ggaaatcgat aaccctcata ctgttccagg
    14641 tctggagcct taatacttcc ggagtcccat gcaggcacgg aagagattca ccatatccca
    14701 ccatatcgta ggcaaatacg gctgcgccca ttaaggcaag ggctgcacac ctgtattgca
    14761 tatcgatcct gtatcgtcca tagtcggcag gattgttcca atgtccgtgc ggacaggcaa
    14821 tacccggtat cttaccggta aatgacgccg gcagatagag atttcctgta acataaaaac
    14881 ccggcaggct ttcaaatgca acgttttcaa cagtgtatcc gtcaaaaacc tgttttatct
    14941 tccttatcgg gttcagattg caccgggcag gcagctcatc aagttttgca cctttcaaaa
    15001 tcgtggccct gatctgcccg gcacgtttca tccatgaagc ctgatcatga tatgttctgg
    15061 aaaatttttc aagattggcc ctgcctcctt cttcagtggt ataataaccc tgacacagaa
    15121 aagattcctg ggaatgggct gaaataaatc caaaaaaaca tgcgaataat aataatttca
    15181 atttcataat catataaact aaatggtttg aaaatctcat acaagataaa tgtttccata
    15241 aagcaattta cataagcagc gattaatttt atatgaggat caataccggg gatattatct
    15301 ctggagtccg tttctacctg tatgaaactg ggttgatgat cctgatcatt ccacataccg
    15361 ggaattgcca tgaccaattc actgtttgca cgttaacagt tcgccattat tgattttcag
    15421 cacagcccgg atcgttagtt tattcatgac cgattcagga cccaaaagca tatgcaggtt
    15481 ctcccttatc cggccggaaa gaagaaggat ccggctaatt accggccaca agcagtttgc
    15541 agctcacctt aaaccgtgaa gtagttaaca cacagaaata catcccctgt ccaatccgtt
    15601 tccccggatc aatgcagcca ttccaggtaa gaatagttaa acccggcccc cttaccccct
    15661 tatcaagggt gagaatcttt aatcctgtca aactgaaaat ggaaaggtcc acgtgatctt
    15721 caccggcaag ttcgtatgcg acagttgcat gccctctgac aggatttgga gcaatcatga
    15781 gtgaaggaag tgcctgctcc gatacttcac tgacatttac caccctgtct tcacgaacct
    15841 gcgcagtatc aagattggtt acatgaatat tgtcaaacca cacatactga cctgaactga
    15901 ccggaaactc tgatgatatt tcaaatcttt ctactgccga ccagtcaaac ttaccttcag
    15961 gatcatacca ggaattattg tcccatgatc ccttctcgta aagatctgac aagggtatat
    16021 ggacatgatg ccatttccgg tcccattctg cgacagtgcc gtcaatcgta atacccatcc
    16081 tccacggatg atcctgttca tcagtctttg tgtcaacgaa cctgatatcg aattttatgt
    16141 cgggcttatt cccacgaacc ataaaatcca ccgcataccc cccggctgca agttttgaca
    16201 gatccttaaa tggcttgaat ctgaaaacga ttgcattata ttgaccaaaa tcctgccaga
    16261 gcaagttata tattccgttg ttaggcagat cagtcgaata gaaattgatt ttgcccgtac
    16321 catagcttcc atctgatatt tgatcgcaga taaaatcaga atatattaaa aatccgttgg
    16381 tttcaggagt aattacaaaa ggagtctggg gtggtatatt taaacccagc gactgaagaa
    16441 gagtgatatt aagatcatga tcaaaaagct catttgagtt tttgttgaat aaaccgaatc
    16501 cgccctggta atcccatatt gtccagggga tatttttttc atcaagatac tgtcttacag
    16561 cgttatacca ataagatctg tcggtattgc cgctgtttgg aatatagaca ccgaattcac
    16621 cgcagaaaat attaacattc ctgctgtccc tgaaatttac cgcaatatcg atcagttgct
    16681 gaacacgggc aaca
//
LOCUS       DAOOYG010000055        16543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3423_length_16543_cov_6.862931, whole genome shotgun sequence.
ACCESSION   DAOOYG010000055 DAOOYG010000000
VERSION     DAOOYG010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3423_length_16543_cov_6.862931"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PK288_06410"
     CDS             complement(<1..410)
                     /locus_tag="PK288_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18589.1"
                     /translation="MSTPDYVVLFGYFILITTFGIWISRRIKTSDSYFRGGRQFKWYI
                     MIGQAFGVGTHAEMPVAQTGATFSNGFATIWYQWKNMLITPFYWLIAPFYRRSERTTV
                     GEIVEDRYGSSMGLVYTIFAILFFVFNMGVMLQGA"
     gene            complement(416..1594)
                     /gene="nagA"
                     /locus_tag="PK288_06415"
     CDS             complement(416..1594)
                     /gene="nagA"
                     /locus_tag="PK288_06415"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPM18590.1"
                     /translation="MTGNSFAEAFHYQTGEYVRIEFSDDGLIREISTVDCAIENQSRI
                     FIAPGLIDNQVNGYMGVDFSEDGLSADRMEMVVEALRGSGISSFMPTIITNSHKNLLR
                     NFRLAVKTLRNSNIRDSIPGFHLEGPYLSPEHGYYGTHPPEFVRKADWNEFLEYQEAA
                     EGNIIQVTIAPEIEGAIGFIEKCTDSGIVVSLGHSNASAEIVNLAVEKGARISTHLGN
                     GLANKIDRHRNPIWPQLANDLLTPSIIADGHHLLREEIIVFTRVKGPDNIFLTSDLSP
                     LGGMPPGTYTYMGSEVIHTPDGFLKNPRMNCLAGASQTIKTGVENMIIFTGCSLPAAI
                     NMASKNVAKACRLNDRGTIETGKRADLILFESAENKINILKTIVKGRIAYTGGTTPNS
                     "
     gene            complement(1616..2350)
                     /locus_tag="PK288_06420"
     CDS             complement(1616..2350)
                     /locus_tag="PK288_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPM18591.1"
                     /translation="MQINISSDALELGTKAARHISAKLKEVIAEKGEARMVVSTGSSQ
                     FEMFQSLIRENVDWARVEIFHLDEYIGLPVTHIASFRKYLYERFINHVAVRKFHSVDV
                     EGNIQEKIERLSAEISKKPVDLGIIGIGVNGHIAFNDPPADFDTRDPYIVVELDDQCK
                     LQQVNEGWFDTVADVPRKAVSMSVWQIMQCDSIISVVPHKVKADAVFRTFTSKLTNLV
                     PATMLKEHKDLTIYLDRNSASKLIAF"
     gene            2479..4656
                     /locus_tag="PK288_06425"
     CDS             2479..4656
                     /locus_tag="PK288_06425"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPM18592.1"
                     /translation="MTRYLVFAFTSVLFLFSIGEIFSQTKGINREAYRIHIKETSEQI
                     SVDGVLDEDVWQVAEKAGHLQRVLPTDTGYAIAQTEVKLTYTPTTLYLGITCFDPLPG
                     KRPVESLRRDFNFGKNDNFIVFIDTFNDQTNGFAFGISPAGAQWDGVQADGGTVNLNW
                     DIKWRSAVKNYDDRWVAEFAIPFRSLRYHGGTPEWGINFSRQDLKTNEKSSWAPMPRQ
                     FATATLAYTGTLVWDKPLPKSGPRFSLIPYVSAKSTQDKLNGKDFNFEPSAGIDAKAI
                     ISTSLNLDLTVNPDYSQVEVDAQQTNLDRFELFFPEKRQFYLENSDLFASLGTETVRP
                     FFSRRIGLDNPVRAGARLSGKLGNKWRLGVMDIQTGNKDTVQASNYMVAALQRQVFSR
                     SSISAFLVNKQVTGGRKGENGQERFNRIGGLEYNLSSPDNKWTGKAFYHHDIHQGTTF
                     NDAAVAANIKYSAQYLSASLDQAAVGKDFNAETGFVRRKGYYEMAPKIGYKFFPHSST
                     IANHGPGIESDLFFNPSGSFSLTDRDTRISYSIEWLNRNVFSIEFQNSYVRLAEPFDP
                     TNTGGTKFNANDDFTWNGIGATFISDVRRLLNFEITGKYGGYYTGTRLYLDGKLYYRV
                     QPYGSLSVVATYNNILLPEPYNSAKLLLIGPRFDFTFTDRLFFTSFIQYNNQIDNLNL
                     NLRFQWRFAPVSDLFIVYTENSYPGNYSVKNRGIVVKLSYWFN"
     gene            complement(4734..5345)
                     /locus_tag="PK288_06430"
     CDS             complement(4734..5345)
                     /locus_tag="PK288_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPM18593.1"
                     /translation="MKIICIGVNYRSHAIEMGRQFPAEPVVFLKPDSAVLKNNKPFFL
                     PDFSANIHYETEVVVRISRLGKGIAPEFAYRYYDEVTAGIDITARDLQSRLSADRLPW
                     TLSKAFDSSAPVGEFLPVSAVGDINNLDFRLEINNVTVQKGNTADMIFGIDKIISFVS
                     GFFTLKTGDLIFTGTPSGVGPLKKNDNLVAYIGDHRVLDFMIK"
     gene            complement(5455..6231)
                     /locus_tag="PK288_06435"
     CDS             complement(5455..6231)
                     /locus_tag="PK288_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPM18594.1"
                     /translation="MELNLRRPLAFIDIETTGINVSSDRIVEISILKIHPNRKEEWIS
                     LRINPEMPIPQSSTSIHGITDADVADAPVFREVAKKLSAFLEGCDLAGFNAIKFDIPL
                     LAEEFLRVNADFSFAKRKYVDVQVIFHKKEQRTLAAAYRFYCGRELQNAHSSKADTQA
                     SYEVLKAQLDMYDDLENNVDKLAEFSSFHNIADFAGRIVYDESGVEIFNFGKHKGKSV
                     ESVFRDDPSYYSWMMNGDFPLYTKKILTEIKLRSFGKGGQ"
     gene            complement(6235..7365)
                     /gene="dnaN"
                     /locus_tag="PK288_06440"
     CDS             complement(6235..7365)
                     /gene="dnaN"
                     /locus_tag="PK288_06440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPM18595.1"
                     /translation="MKFVVSSTELLGHLQAISRVISSKNTLPILDNFLFNLSGNDLEI
                     TASDLESTLITRMKLENASGDGIIALPARILLDTLREFPVQPLSFDINLETMAVVITS
                     ENGKFNVMGQNGIDFPALPAIMKEKRFTFQINADVLLSGINRTLFATADDELRPVMGG
                     IFIEASDDRITFVASDAHKLVRYQRTDAGADAGSSFILPKKPASLLKNILPKEEGPVT
                     VEFDDKNAFFNLNDYKVVCRLIEGNYPNYSSVIPKNNPRKITIDRVEFYNTLRRVSVF
                     SNQASNLVKLQLKGNQVMVSAQDIDFSISAYEKIKCQYEGDDMEIGFKSVFLLEILAN
                     ISSQDVIIELADPTRAGLFLPGEADNQAEDLLMLLMPMMINA"
     gene            complement(7504..8472)
                     /locus_tag="PK288_06445"
     CDS             complement(7504..8472)
                     /locus_tag="PK288_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18596.1"
                     /translation="MTGKSLKYLSVIVILIAVFLFLFRDRDPFGRKNSSFASKPKEEI
                     TRIELSDKSSSISLTRKDNIWMLNGSHEVRKSSISFLIQVVSAMEIKSPVSPELFRAE
                     IREKGIEPVKVSVFGKRKRLNSFLVYKTQSNIYGNIMKKREGAKPFIVYLPGYETNIG
                     SIFTVNELYWKPFMVFNQLPSDIASVNFENMTDTASSFSIQKEGGRFILSDKGRELSG
                     WDTSRVIRYISYFAYIPFESWELEIDPAEQKTITDSGPLCIISLVTTAGENIRLTLWE
                     RFLNDGREKIKDTDRLWAKTDSNTALFIIRYMDIDPLLKKRSYFYP"
     gene            complement(8475..10193)
                     /gene="gldG"
                     /locus_tag="PK288_06450"
     CDS             complement(8475..10193)
                     /gene="gldG"
                     /locus_tag="PK288_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HPM18597.1"
                     /translation="MAGSNSNIRSIRRKILTEFLVAVALIVFVAAILSYFRLRLDLTG
                     DKRYSLSVPTRKVLSGLRNDVFIQVYLDGEMPIPFKRLKRSVREMLDEFRIASGNRVD
                     YAFLNPSEGTDRKQREARYQSLIGKGLNPVNIQASDEEGGASQKIIFPGMIVNYNGIE
                     VPVNFLKNNPSLPAEENLLHSVEGLEYEMIQTISTLTSDTIYKIAFIEGHGELSEPQV
                     ADITLSLARFFTIDRGKLTDDPGILDNYAAVIIAGPQNEFPESDKFILDQYIMKGGKV
                     LWLLEEVDVNADSLVSGETEALIRTLNLEDQLFRYGARINPAVVQDVECVMIRLALMT
                     SGTRQQFVPAPWFYYPRLYPSQDHPVTRNLNRVKGEYVNYIDTVGLDGRIKKTVLLST
                     SGFSRILSPPFMISLKDAEITPDEREFNRSKLPVALLLEGVFPSAFRNRMTGDLTGDK
                     SFKPRNESIPTKMIVIADGDIIRNEVRQIGSSVSHYPLGQDIYTGEMYGNRDFIINCL
                     NYLVDDNGIMELRSRELKLRMLDRTRIKNEKLKWQLVNILLPPLLAIVSGLIFTYFRK
                     RRYTKP"
     gene            complement(10194..10937)
                     /gene="gldF"
                     /locus_tag="PK288_06455"
     CDS             complement(10194..10937)
                     /gene="gldF"
                     /locus_tag="PK288_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HPM18598.1"
                     /translation="MFAIFRKEISGFFSSLTGYIVIIVFLLINSLFMWVFPGELNVLE
                     SGYAGLDTLFLISPWVFLFLVPAITMKMFAEEKKLGTIELIYSRPVSERRIVYGKYLA
                     SVALAFLSLLPCIIYYISVSRLVETPGNIDKGAVLGASAGLLCLAAVYAAAGLLASSF
                     TDNQVIAFIIAVLFAFFLFMGFDSFAYLPGLKKVDEFVIRLGINEHYKSMSRGVVDLS
                     DLVYFAAVVLIFNEATRLVLLSRKWREKS"
     gene            11238..14318
                     /locus_tag="PK288_06460"
     CDS             11238..14318
                     /locus_tag="PK288_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM18599.1"
                     /translation="MSETVIEALVRLFALISDIHDENTVISRKKELVRLFLERHLNKE
                     MVTRYMMQFEIFLEQYYSESIERGTIRERKRVALNSIKILAICEKINTELYQKQKIYV
                     IVQLLDFISYGEDITGTESEFVDTVASAFNIPDDEYANIRQFILQDVNNVRDKSKILT
                     INNRKEAVHPEIRHLYDSNLSGNISFLLIPTTLTYIMRYKGLEDLYLNGQVVFPNQTY
                     IFDQGSSIRGAGIKTVYYSEISGLIAETSRKFRISLDAINISFKFTDSQYGLHNLNFH
                     GEGGNLIGIIGGSGVGKSTTLSILNGTLKPQSGEVLINGYNIYDENDREALRGVIGYV
                     PQDDLLIEELTVFQNIWFNARLCLNNLNESEITDAVNNILVDFDLQEIKDLKVGNPLK
                     KVISGGQRKRINIALELLREPTILFVDEPTSGLSSVDSEVVMNLLKAQTHKGRLVVVN
                     IHQPSSEIYKMFDRIMIIDRGGYQVFFGNPSKAITYFKTQTNHANPKEDQCIKCGNVN
                     TDQILQIIEAKVVDEHGKMTRTRRVTPAEWAEKFVQSGAVSIRREDVKKQEIPRNNFN
                     IPGIFKQSVIFLTRDILSKLADKQYILISLLGSPVLAFFLAYFTRSARGDIYHFSEND
                     NIPAYLFMAVITSLFFGLMLSSEEIIKDRKILKREAFLNLSWFSYLNSKIMIMFILSA
                     IQTLSFAIIGNAVLGIKGMTLSYWLVLFTTSCFGNILGLNISSVLNSVITVYIIIPFI
                     IIPQLLFSGVLVKFDKLHINSKSAHEFVPVIGDVMTARWSFEALAVKQFRDNRYERNF
                     FVHDMAESQNNYYSSFLIDELNRELYVCNLYRDSSNYRNSVLNSFERLRFHIGELSEI
                     SGISPGSWRDSISLELSGSSVEKKAAAYLATLKKYFTDRQKEARLARDTVSRSLLDRY
                     GEEKMLEMRNNYENKQIKRIVLDADNLDKTYRLPGKIVQKADPGYMVAAARNGRAHFY
                     APCKRLGNLKIDTYFFNIMVIWLESIVLYFTLYTNIFRKLFTFIGSLRRDMTET"
     gene            complement(14362..15510)
                     /locus_tag="PK288_06465"
     CDS             complement(14362..15510)
                     /locus_tag="PK288_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPM18600.1"
                     /translation="MLRVVISIQVFLAFFALQASGQVKIRLFAAKPLHWIVFNVNEGS
                     YRLDFYDDAPIYLKKGDNAIVSFYNGKVAVKTKGDSGFTCDSMVFTGQTGQDLFSVRV
                     GNEPATGRIYDGDLQCIHDLGFLVLINTCNIDRYIAGVVQAEGGTGKNIEYIKSQALI
                     ARTYMYRYLNKHIIDGYNLCDGTHCQAYMGNSQDPAINRAVLETTGLVVLGRDSLPIM
                     SAFHSNCGGETESSENVWLSGQPYLKKVVDPYCTGSRNAKWTKSITVKEWKDYLRNSG
                     FTTPEAGKPDLNFSQITRQKDYRTGSFSLPFTKIRDDFNLKSSFFSVVQSGDSMIFRG
                     KGYGHGVGLCQEGAMVMAAQGKNYRQILQFYYSNIIISDIRNAKNPAQ"
     gene            complement(15596..>16543)
                     /gene="lpxB"
                     /locus_tag="PK288_06470"
     CDS             complement(15596..>16543)
                     /gene="lpxB"
                     /locus_tag="PK288_06470"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPM18601.1"
                     /translation="RKNISLCKSQIISSGPDLLILIDYPGFNLRIAEWAKKSGFRVFY
                     YISPKLWAWNEKRVKKVKKYVDRMYIIFPFEETFYDKHNIRAIYCGNPLLDETEVKLT
                     SLPDKDKCKRSLGLDDRPVIALLAGSRKSEVKHILPKMLEVIPDFKDYRFIVAGVKNL
                     PETLYSDVAGSLDLQVIRDRTYEILHISDAALVASGTATLETALFDVPQVVCFRGDLL
                     SMLIAWMVIRVKYISLVNLIAGEEIVKELVQYSLNRRNLVRELSSILPGGPGRRKMLD
                     GYNKVKKILGPSGASSRAAEDIVKTLSGFMRAGNDQSVD"
BASE COUNT         4613 a         3898 c         3508 g         4524 t
ORIGIN      
        1 gctccctgca gcatcactcc catgttgaac acaaagaaca atattgcaaa tattgtataa
       61 acaagtccca ttgaacttcc gtatctgtcc tcaactattt ctcccacggt ggtgcgctcg
      121 cttctcctgt aaaaaggagc aatgagccag tagaaaggtg ttataagcat attcttccac
      181 tggtaccata tcgtggcaaa tccattgctg aaagtagcac cggtctgggc aacaggcatc
      241 tcggcatggg tccccacacc aaatgcctgg ccgatcataa tgtaccactt aaactgtctg
      301 cctcccctga aataactgtc gcttgtcttt attcgtctcg atatccagat gccaaatgtg
      361 gttatcagaa taaaatatcc gaacaatact acataatcag gtgtactcat aaggctcagc
      421 tgtttggtgt ggttccgcca gtataggcaa ttctgccctt cactatggtt tttaagatat
      481 taattttatt ttcggctgat tcaaacagga tcagatctgc ccgtttcccg gtttcaattg
      541 ttccccggtc atttagccta caggcctttg ccacgttttt cgatgccatg tttatagcag
      601 caggcagtga acatcctgta aaaataatca tattctccac acccgttttt atggtctgtg
      661 aggcacctgc aaggcaattc atccttgggt ttttcaggaa accgtcgggg gtgtggatta
      721 cttcactgcc catgtaggtg tatgttccgg gaggcattcc gcccagcggg cttaaatcag
      781 aggtgagaaa aatattatcg ggccctttta cccttgtgaa cacgataatt tcttccctca
      841 ggaggtgatg tccgtcagca attatcgagg gagtcagcag gtcatttgcc agctggggcc
      901 agatgggatt ccggtggcgg tctatcttgt ttgcaagtcc gttcccaaga tgagtcgaaa
      961 ttcttgcgcc tttttccaca gccagattca ctatctctgc tgatgcattt gaatgcccca
     1021 gtgaaaccac aattccgcta tcggtgcatt tttcaataaa tccgattgca ccctcaattt
     1081 cgggagcaat ggttacctgt ataatatttc cctctgccgc ttcctggtat tccagaaatt
     1141 cattccagtc agccttcctg acaaactccg gaggatgagt gccataataa ccatgttcag
     1201 gcgaaagata ggggccctcg agatggaaac caggaattga gtcacggata ttactatttc
     1261 tcagtgtttt aacggccagc ctgaaatttc tgagcagatt cttatggctg ttggtaataa
     1321 tggtgggcat gaatgaactg attccactcc cacgtagtgc ttcaacaacc atttccatac
     1381 ggtcggcaga gaggccgtct tcagaaaaat caacccccat ataaccgttt acctggttat
     1441 caatcagccc gggtgcaata aaaatccggg attgattttc gatcgcgcaa tcaacggtgc
     1501 tgatttccct gatcaacccg tcatcgctga actcgatcct cacatattcc cccgtctgat
     1561 aatgaaaagc ctctgcaaag ctgttgcccg tcatgaaata gccggaattt aatgattaga
     1621 atgcaattaa ttttgatgcg gaattccggt ccagatatat ggtgagatcc ttatgttcct
     1681 taagcatggt tgccggcaca agattggtca gcttactggt gaaagtcctg aacactgcat
     1741 ctgctttaac cttgtgggga acaaccgaaa taattgaatc gcattgcatt atctgccaga
     1801 cactcatgga aacggctttc cggggcacat ctgcaacggt atcgaaccat ccctcattta
     1861 cctgttgaag cttgcactga tcgtcaagtt ccacgactat ataaggatca cgggtatcaa
     1921 aatcggcagg aggatcgttg aatgcaatat gcccgttcac gcctatacca atgatgccaa
     1981 ggtcaacggg ttttttgctt atttcagctg aaagccgttc aatcttttcc tgaatgttcc
     2041 cttcaacatc cacactgtga aatttcctga cagcgacatg atttataaaa cgctcataca
     2101 ggtatttcct gaaactggca atatgggtaa caggcaatcc tatgtactca tcgaggtgaa
     2161 atatctcaac cctggcccag tctacattct ctctgatgag gctctggaac atttcgaact
     2221 gggagcttcc ggtagacacg accatcctgg cttctccctt ttctgcaatt acttccttca
     2281 gttttgctga aatatgcctg gctgcctttg ttccgagctc cagtgcgtcg ctgctaatgt
     2341 ttatttgcat ctcttttttt gcttaaaaat aaaaaataaa actaactttc gggccttttg
     2401 gttaaaagtt aaaagacgtt aacgataata gcagctcccg gaaatcattc taaattacat
     2461 cgtcaatttt tgttacacat gaccaggtat ttggtttttg cttttacatc cgttcttttc
     2521 cttttcagca taggagaaat cttttcccag acaaaaggta tcaaccggga agcatacagg
     2581 atccatataa aggaaacctc cgaacaaata tcagttgacg gtgttttgga tgaggatgta
     2641 tggcaggttg cggaaaaagc cggacatctg cagagggttc tgcccactga caccggttat
     2701 gcaattgccc agacagaagt gaagcttaca tacactccaa ccacacttta cctggggatt
     2761 acctgttttg atccactccc ggggaagcgc cccgtggaat cactccgccg cgatttcaat
     2821 ttcgggaaaa atgacaattt cattgttttt atcgatacat tcaatgacca gacaaatggt
     2881 tttgccttcg ggatctcacc ggccggggca caatgggacg gcgttcaggc agacggaggc
     2941 accgtaaacc taaactggga tataaagtgg agatcagctg taaagaacta tgatgaccgc
     3001 tgggttgccg agtttgcaat acctttcaga agtttgaggt accacggcgg aacgcctgaa
     3061 tggggaataa acttcagcag gcaggacctc aaaacaaatg agaaatcaag ctgggcaccg
     3121 atgccaaggc agtttgcaac cgcaaccctc gcatacaccg ggacgctcgt atgggataaa
     3181 cctctgccca aatccggacc ccggttctcg cttatacctt atgtgtcggc aaaaagtact
     3241 caggataaac ttaacggaaa ggattttaat ttcgaacctt ccgccggaat agatgcaaaa
     3301 gcaattattt caacctcgct caatctcgat cttacagtaa atcccgacta ttcacaggtg
     3361 gaggtggatg cccagcaaac caacctcgac cgctttgaat tgttctttcc ggaaaagcgt
     3421 cagttctatc tcgagaacag cgatttgttt gcaagcctgg gaactgaaac cgtcaggcct
     3481 tttttttcaa gaaggattgg tctcgataac ccggtaaggg caggcgccag gctcagcggc
     3541 aagcttggaa acaaatggag actgggagtt atggatatcc agactgggaa caaagatacc
     3601 gttcaggcat cgaattatat ggtggcagct ttgcaaagac aggttttcag ccgttcaagt
     3661 atttccgcct tccttgtaaa caagcaggtt acggggggca ggaaagggga aaacgggcag
     3721 gaaaggttca acaggatagg cggtctggaa tacaaccttt caagcccaga caacaaatgg
     3781 acgggcaagg cattctatca tcatgatatt caccagggca caacattcaa cgacgccgcc
     3841 gtggccgcaa atatcaagta ttccgcccag tatctgtcag cttcccttga tcaggctgca
     3901 gtcggcaaag atttcaatgc cgaaaccggt tttgtaagaa gaaagggata ttatgaaatg
     3961 gcgccgaaaa tcggctataa gttttttccg cattcaagca ccatcgccaa tcacggaccg
     4021 ggaatagaat ctgacctgtt ctttaatccg tccggatcat tttcgcttac cgaccgggat
     4081 accaggatct cctacagcat agagtggctc aacaggaacg tattttccat cgagttccag
     4141 aattcatatg tcaggcttgc tgaacccttc gaccccacaa acaccggagg aactaaattc
     4201 aatgccaacg acgattttac atggaacggg atcggagcaa ctttcatatc ggacgtacgc
     4261 aggctgctga actttgaaat aacaggcaaa tacggaggtt attacaccgg gaccaggctg
     4321 tatcttgacg ggaaattgta ctaccgtgtc cagccctatg gaagcctgtc tgttgtcgcc
     4381 acatataaca acattttact gcctgaacca tacaacagtg caaaactgct tctgataggt
     4441 ccaagattcg acttcacctt taccgacagg cttttcttta caagcttcat ccaatacaac
     4501 aaccagatcg acaatcttaa cctgaatctc cggttccagt ggcggtttgc tcccgtctcg
     4561 gacctgttca tcgtatacac cgagaactca taccctggaa attacagtgt taaaaacagg
     4621 ggaattgttg tcaagctttc atactggttc aattagatcc tgccttcccg atcatgggga
     4681 accacgcggg gaaagagggc tgtcactgat tggtttccgg cataaccatc tgtttattta
     4741 atcataaagt caagtacccg atggtcgccg atatatgcca cgagattgtc attttttttc
     4801 aggggtccta cgccagaggg tgttcctgtg aatatgaggt caccggtttt aagggtgaaa
     4861 aatccggaaa caaatgagat tattttatct atcccgaaaa tcatgtctgc agtattaccc
     4921 ttctgcactg tgacattgtt tatctcaagc cggaaatcga gattatttat atctccaacg
     4981 gcgcttaccg gaaggaattc accaacagga gctgaactgt caaaagcctt ggaaagggtc
     5041 cagggcagtc tgtctgctga caggcgtgac tgcagatcgc gagccgtgat atcaatacct
     5101 gcggtaactt catcgtaata acgataagca aattcaggag caattccttt tcccagcctg
     5161 gaaattctga caacaacctc tgtctcataa tggatattgg ccgaaaaatc gggaagaaag
     5221 aaaggcttgt tattcttcag aacggctgaa tcgggcttca gaaaaactac cggttcagca
     5281 ggaaactgcc tgcccatctc aatggcgtga cttctgtaat tgactcctat gcaaataatt
     5341 ttcataacta actgcttctg ttcccgtttt cacatatcgg gttatcaacg tgcggcaatc
     5401 tgcacctggc gaatactgtt caacagtggc gacctgatcc atggagctga caagctattg
     5461 acctcccttg ccaaacgacc tgagcttgat ctccgtaaga atcttcttgg tataaagtgg
     5521 aaaatccccg ttcatcatcc atgaataata tgaagggtca tccctgaaaa cactttcaac
     5581 ggacttgcct ttgtgcttgc cgaaattaaa tatctcaaca ccgctttcgt cgtaaacaat
     5641 ccttccggca aaatcagcaa tattatggaa cgaactgaat tcagcaagct tgtccacatt
     5701 gttttccagg tcatcataca tgtcgagctg tgccttgaga acttcatagg aggcctgggt
     5761 gtcggctttt gaactatggg cattctgaag ttcccggccg cagtaaaacc ggtatgccgc
     5821 tgccagggtt cgttgctcct ttttatgaaa gatcacctga acgtcaacat attttctttt
     5881 tgcgaaactg aaatcggcat taactctcag gaattcttcg gcaagaaggg gaatgtcgaa
     5941 ctttatggca ttgaatcctg ccagatcgca tccttcgaga aaggcggaga gcttttttgc
     6001 cacttccctg aatacagggg catcagctac atccgcatca gtgattccgt gtatgctggt
     6061 agaactctga ggaatgggca tttccggatt gattctcagg gagatccatt cctctttcct
     6121 attcggatgg attttaagga tggaaatttc cacaatacga tcggacgaga catttattcc
     6181 cgtcgtttcg atatcaataa aagccagggg gcgcctaaga ttcagttcca actgtcaggc
     6241 atttatcatc attggcataa gaagcataag gagatcctcc gcctggttgt cggcctcccc
     6301 gggaaggaac agccccgccc ttgtaggatc tgccagttcg attatcacat cctgcgaaga
     6361 aatatttgcc agtatctcaa gcaggaatac tgatttgaat cctatctcca tatcatcgcc
     6421 ttcatactgg catttgatct tctcataggc agatattgaa aaatcaatat cctgtgcgga
     6481 taccatgacc tgatttccct ttagctgaag cttaacaagg ttactggcct gatttgaaaa
     6541 aactgatacc ctcctgagag tattgtagaa ttcaaccctg tcgatggtta tcttcctggg
     6601 attatttttg ggtatgactg aactgtaatt cggatagtta ccctcaatca ggcgacaaac
     6661 caccttataa tcgttcagat tgaaaaatgc gtttttgtca tcgaactcaa cagtcacagg
     6721 tccctcctct ttaggaagga tgtttttcag gagggaggcc ggtttttttg gcaggataaa
     6781 ggaggagcct gcatcggcac cggcgtctgt cctctggtat ctgacaagct tgtgagcatc
     6841 cgaggcaaca aaggttatcc tatcgtcaga agcttcaatg aatatgcctc ccattacggg
     6901 ccgcagctca tcgtccgccg ttgcaaacaa cgtccggttt atgcctgaca gcaaaacatc
     6961 cgcattgatc tgaaatgtaa atctcttttc cttcattatt gcggggagtg caggaaaatc
     7021 aatcccgttc tggcccataa cattgaactt gccgttttcc gaagtaatta cgacagccat
     7081 ggtttcaagg tttatatcaa atgaaagcgg ctggaccggg aattctctca gggtgtcaag
     7141 cagaatcctc gcaggaagtg caatgatccc gtctcccgaa gcattttcaa gcttcattct
     7201 ggtaataagt gttgattcaa gatcagatgc cgttatttcc aggtcattcc cggatagatt
     7261 aaaaaggaaa ttatcaagaa tgggaagggt gttcttcgaa ctgatcaccc tgcttattgc
     7321 ctgcaaatga ccaagaagct ctgtactgga tacaacaaat ttcattttat aggatgattt
     7381 aatttaattt cttttcagga attagtacat atagctgtca ttaaacaggt taaaaggatg
     7441 gctgtttatg gcaactaact atcaatatta caaaaaaaac tattaacctc aaataaaaag
     7501 ttttcaggga tagaaataag atctcttttt caggagagga tcaatatcca tatatctgat
     7561 tataaacagg gcggtattgg aatccgtttt tgcccacagc ctgtcggtat ctttaatctt
     7621 ttccctgcca tcattgagaa atcgctccca aagtgtcagc cgtatgtttt ccccggcagt
     7681 ggtcacaagt gagataatac aaagcggacc tgaatcagta atagtctttt gttctgcagg
     7741 atcaatctca agttcccacg attcgaacgg aatataggca aaatatgaaa tataccggat
     7801 tacccgcgaa gtatcccagc ctgaaagctc cctgcccttg tctgaaagaa tgaatctccc
     7861 gccttccttt tgaattgaaa atgaagaagc cgtatcggtc atgttttcaa aattcactga
     7921 tgctatatcc gaaggaagct gattgaaaac cataaatggc ttccagtaaa gttcattgac
     7981 ggtgaagatt gagccgatat ttgtttcata tccgggaaga taaacaataa acggttttgc
     8041 tccttcccgt tttttcataa tgttcccgta aatgttcgac tgggttttgt agaccaggaa
     8101 agaattaagt ctcttccgtt ttccgaaaac actgactttc accggctcta tccccttctc
     8161 cctgatctcg gccctgaaca attcgggcga aactggtgat tttatttcca tcgcggaaac
     8221 gacctgaatt aggaaagaga tgctgctttt ccttacctca tggctgccat tgagcatcca
     8281 gatgttatct ttcctggtca gactgatgga tgagcttttg tcagataact ctatcctggt
     8341 aatctcctct tttggctttg aagcaaaact gctgttcttc ctgccgaaag gatccctgtc
     8401 cctgaacagg aaaagaaata cggcaatcag aattacaata accgataggt attttaaact
     8461 tttgcctgtc ataattatgg cttagtatat ctccttttcc tgaaataggt gaaaatcaat
     8521 ccggaaacaa ttgccagaag cggggggagg agaatgttaa ccagctgcca tttgagcttc
     8581 tcatttttga tccttgtgcg gtcaagcatt ctcagtttca gctcacgtga gcgaagctcc
     8641 ataattccat tgtcgtcaac aagatagttc aggcaattaa tgatgaaatc gcggtttccg
     8701 tacatttcac ctgtatagat atcctgacca agcgggtaat gggatacact gctgcctatt
     8761 tgtctgactt cattcctgat tatgtcgcca tcggcaatga ctatcatttt tgtaggaatg
     8821 ctttcgttac ggggtttaaa gcttttatcg ccggtaaggt ctccggtcat cctgtttctg
     8881 aatgctgacg ggaacacccc ctcaagcagc agggcaaccg gtagttttga tctgttgaac
     8941 tctctctcat ccggtgttat ctcggcatcc ttcagactta tcataaaagg aggagacagg
     9001 attcttgaaa atcccgaagt tgagagcagg actgtttttt taattctgcc gtcgaggccc
     9061 accgtatcaa tataattgac atattctccc ttcaccctgt taagatttct ggtaacagga
     9121 tgatcctgcg aggggtaaag tctgggataa taaaaccatg gtgcggggac aaactgctgg
     9181 cgggtgccgc tcgtcatcag ggcaagcctt atcataacgc attccacatc ctgaactact
     9241 gccgggttta tcctggcacc atacctgaaa agctgatcct ccagattcaa tgtacggata
     9301 agagcttcgg tctcccccga aaccaggctg tcagcattta cgtcaacctc ctccagaagc
     9361 cacaaaacct tacccccttt catgatgtac tgatcgagaa taaatttgtc gctttccgga
     9421 aattcattct gaggtccggc aataataact gcagcatagt tgtcaagaat gcccggatca
     9481 tctgtcagtt ttcccctgtc aatagtgaaa aaccttgcca gactgagcgt tatatcagca
     9541 acctggggct ccgaaagctc accgtgaccc tctataaagg caatcttata tattgtatcg
     9601 gatgtcaggg ttgaaatggt ctggatcatc tcatactcaa ggccttcaac tgaatgcagc
     9661 agattctcct cggctggtag cgaaggattg tttttcagga aattgacagg cacttcgata
     9721 ccattataat ttacaatcat gcccggaaat attattttct ggctcgctcc cccctcctcg
     9781 tctgaggcct gaatattcac aggattgagc cccttgccta tcagtgactg atacctggct
     9841 tccctctgtt tccggtccgt cccttccgaa ggattcagga atgcataatc gaccctgttg
     9901 cctgatgcta ttctgaactc atcaagcatt tcccgcaccg aacgtttcag ccgcttgaag
     9961 gggatcggca tctctccgtc gagatatacc tgtatgaaaa catcattcct taacccggaa
    10021 agcactttcc gggtaggaac ggaaagagaa taccgtttgt cccctgtcag atcgagcctc
    10081 agtctgaaat aggacaatat cgctgcaacg aatacgatta gtgccactgc taccaggaac
    10141 tccgtcaaaa ttttccttct gattgaccgt atgttggaat tactgcctgc cattcagctt
    10201 ttctccctcc acttccgtga taaaagcaca agcctcgttg cctcattgaa aataagaaca
    10261 accgcagcaa aatacaccag atcacttaaa tcgacaacac ctctgctcat tgacttgtaa
    10321 tgttcgttta ttccaagtcg tataacgaac tcatcaactt ttttcagtcc gggcagataa
    10381 gcgaaagaat caaatcccat gaacaggaaa aatgcaaaaa gaactgcgat gataaaagca
    10441 ataacctggt tatcggtaaa tgatgaagca agaagcccgg cggcggcata taccgcagca
    10501 agacaaagaa gaccggctga tgcaccaaga actgcacctt tgtcgatatt tcccggtgtc
    10561 tctacaagcc tcgagaccga aatataatag attatgcatg gcagcaatga cagaaaagca
    10621 agggcaacgg atgccaggta tttaccatat actattcttc tttcacttac cggccttgaa
    10681 taaataagtt caatcgtgcc gagtttcttt tcctcggcaa acattttcat tgtaatagcc
    10741 ggcaccagga aaagaaacac ccagggagag atcaggaaca gggtatccag gcctgcatat
    10801 ccgctttcaa gaacattaag ctcacccggg aaaacccaca tgaacagtga attgatcagc
    10861 aggaaaacga ttatgacaat atagcctgtc aggctgctga aaaatccgct tatctctttt
    10921 ctgaaaattg cgaacatgta attaaacaaa cggcaaaaat aaacaaataa gcttaatcaa
    10981 aattacaatc tttcaaccaa cccggatctt tccagcatgc tgccagcctg ccattttcct
    11041 ggttctcatt gtcttaattt ttcttaaacg ccagatccgc cggatcgttc cgggtagtga
    11101 taacccctga aataaaatgt ttttttattt atgagaaaat actaacttca aatgtttcag
    11161 acaaaattaa tctttaaaag cttcttacgg ataaaagaga gcttttcatt cctgatctgc
    11221 tgatatattt ttaaactatg agcgaaacag ttattgaggc cctggtacgt ctttttgcgc
    11281 ttatcagcga tatccatgac gaaaacactg taatcagccg caaaaaggag cttgtcaggc
    11341 tctttcttga acgccattta aacaaggaaa tggtaacaag atacatgatg cagtttgaaa
    11401 tctttcttga acaatattat tcggagagca ttgaaagagg gaccatcagg gaacggaagc
    11461 gtgttgcact gaattcaatt aagatcctgg caatctgtga aaagattaac actgaactgt
    11521 atcagaaaca gaaaatttat gttattgtgc agcttcttga ctttatctcc tatggtgagg
    11581 atattaccgg aaccgaatca gaatttgtag atactgtcgc cagtgctttc aatattcctg
    11641 atgatgaata tgcaaacatc aggcaattca ttttgcaaga cgtgaataat gtcagggaca
    11701 aaagcaagat actgacaata aacaaccgta aagaagctgt tcatcccgaa ataaggcacc
    11761 tttatgactc aaacctttcg gggaacatct cattcctgct cattccaacc acgcttacct
    11821 atattatgag gtacaagggg cttgaggacc tgtatctgaa cgggcaggtt gtttttccga
    11881 atcagactta catattcgat cagggttcat caataagggg agcaggtatc aaaactgtct
    11941 attacagcga aataagcgga ctgattgccg aaacatcacg gaagttcagg atatctctcg
    12001 acgcaattaa catatcattc aaattcaccg acagccagta cgggctccac aacctgaatt
    12061 ttcacggcga gggagggaac cttatcggga tcattggcgg aagcggtgtg ggcaaatcca
    12121 caactctcag cattcttaac ggaacattga agccgcaaag cggtgaagtt ctcattaacg
    12181 ggtacaatat ttacgatgag aacgacaggg aagcactaag gggagttata ggctatgtcc
    12241 ctcaggatga ccttctgatc gaagagctga cagtgttcca gaacatatgg tttaatgcaa
    12301 ggctctgcct gaataatctg aatgagagtg aaataaccga tgcggttaac aacatccttg
    12361 ttgattttga tctccaggaa ataaaggatc tcaaggttgg aaatccactt aaaaaagtaa
    12421 tcagcggcgg acagagaaaa aggatcaata ttgcgcttga acttctgcgg gagcccacca
    12481 tactgtttgt agatgaacct acgtcaggat tgtcttccgt cgactccgag gtggtaatga
    12541 acctgcttaa ggctcagacc cataaaggca ggcttgttgt agtaaacata caccagcctt
    12601 cctccgagat ctataaaatg ttcgacagga ttatgatcat tgacagggga ggttaccagg
    12661 tttttttcgg aaatccttcc aaagcaatca cttatttcaa gacccagacc aatcatgcga
    12721 atccgaagga ggaccagtgt attaaatgcg gcaacgtaaa taccgatcag atcctccaga
    12781 tcattgaggc caaagtggtt gacgaacatg gcaagatgac ccggacaaga agggtgactc
    12841 ctgctgaatg ggctgaaaaa ttcgtgcaat caggagcagt ttccatcaga agggaagatg
    12901 tgaaaaagca ggagatcccc cggaacaatt tcaatatccc gggaatattt aaacagtcag
    12961 taattttcct tacgcgcgat attttatcaa agctggcaga taaacaatat atcctgatca
    13021 gtcttttggg atcccccgta ctggctttct ttcttgcata ctttacaagg tctgcccggg
    13081 gcgatattta ccacttcagt gaaaatgaca atatccccgc atacctgttc atggctgtaa
    13141 taacctcgtt gtttttcggg ttaatgctca gctcggaaga gatcataaaa gacagaaaga
    13201 tcctgaaaag ggaagccttt ctcaacctga gctggttcag ttatctgaat tccaaaataa
    13261 tgatcatgtt cattctttcg gcaatacaaa cgttatcctt cgcaataatc ggcaatgccg
    13321 tactcggtat aaaaggaatg actttaagtt actggctggt gctgttcacc acatcatgct
    13381 ttggcaacat cctgggcctg aacatctctt cagtcctgaa ttccgtgatt acagtctaca
    13441 taataattcc tttcataatc attccccagc tgcttttcag cggggtcctg gttaagtttg
    13501 acaagctgca tatcaacagc aagtcagctc atgaattcgt tcccgtcata ggggatgtca
    13561 tgacggcccg ctggtcgttt gaagcactgg ctgtcaaaca gttccgggac aaccgatatg
    13621 aaaggaactt cttcgtccat gacatggctg aaagccagaa caactattat tcatccttcc
    13681 tcatcgacga actgaacagg gagctgtatg tctgcaacct ctacagggac agcagcaact
    13741 acaggaacag cgttctgaac agctttgaaa ggctcaggtt tcatatcggt gaactgtcgg
    13801 agatatcggg gatcagtccc gggagctggc gtgattcgat ttcactggaa ctttccggca
    13861 gcagtgttga gaaaaaagca gctgcttatc ttgctacttt gaagaagtat tttaccgaca
    13921 gacagaagga agcaagactg gccagggaca ccgtcagccg ctccctgctg gacagatatg
    13981 gggaggaaaa aatgcttgaa atgcgcaata actatgaaaa caagcagata aaaaggattg
    14041 ttctcgatgc cgacaatctc gacaaaactt accggcttcc cggcaaaatc gtgcaaaaag
    14101 ccgatcccgg atatatggtt gctgcagcac gtaacggaag ggcccatttt tatgctccct
    14161 gtaaacggtt aggcaacctg aagatcgaca cctacttttt caatataatg gtaatatggc
    14221 tggagtccat tgtattgtac tttacactct acacaaacat tttcaggaaa ctattcacct
    14281 ttatcggcag tctgcgccgt gatatgacgg aaacatgaaa aacaggcatc attttcatta
    14341 tttgcaacag cgtttacttt tttactgcgc cggatttttg gcatttctga tgtcggaaat
    14401 aatgatattg gaataataga actgcaatat ctgcctgtaa ttttttcctt gtgcggccat
    14461 aaccatggca ccctcctggc aaaggccaac accatgaccg taaccctttc ccctgaaaat
    14521 cattgaatca ccgctctgaa cgactgaaaa aaaagaggat ttcaagttga aatcatccct
    14581 tatttttgtg aatggcaatg aaaacgatcc cgtacggtag tctttctgtc gcgtaatctg
    14641 ggagaaattc aagtcaggtt ttcccgcttc gggtgtagtg aatcctgagt ttctcaggta
    14701 atctttccat tccttaaccg ttatgctttt tgtccatttt gcattcctcg agcctgtaca
    14761 atacggatca accactttct taaggtaggg ttgtcccgag agccagacat tctccgatga
    14821 ctctgtttca cccccgcagt tggaatgaaa tgccgacatt attggcaggc tgtcgcgtcc
    14881 cagcaccacg agccctgttg tctcaagaac tgccctgttg atggcaggat cctgtgaatt
    14941 acccatatag gcctgacaat gtgtaccatc acacagattg tacccgtcaa tgatatgctt
    15001 gttcaaatac ctgtacatat aggtccttgc aatcaaagcc tggcttttta tatattcaat
    15061 attctttccc gtccctcctt ctgcctgcac cacaccggca atatacctgt cgatgttaca
    15121 ggtatttatc aaaaccagga aacccagatc gtgaatgcat tgcagatcgc cgtcataaat
    15181 ccggcctgtc gccggctcat tgccaaccct cactgaaaac aggtcttgcc cggtctgacc
    15241 tgtaaatacc atggaatcgc aggtaaaacc gctgtcacct ttggtcttca ctgcaacttt
    15301 tccattgtaa aatgagacga tcgcattatc ccctttcttc agataaatcg gggcatcatc
    15361 gtaaaaatcc agccggtaac ttccttcatt gacattaaac acgatccagt ggaggggttt
    15421 tgctgcaaac agccgtatct taacctggcc cgaagcctga agtgcaaaaa aagccaggaa
    15481 tacctgtatt gaaattacga ctcttagcat tgcattcagt attatgtccc gtccggcgtc
    15541 atatcacctg caaatggaac ttatgatgat cttgccgttc aatagatatt aaatgtcagt
    15601 caacagattg atcattaccc gcgcgcatga atccggagag ggtctttaca atatcttcag
    15661 ctgccctact ggatgcaccg gaaggtccca ggatcttttt caccttgttg tagccatcaa
    15721 gcatctttct gcgtcccgga ccgcccggga gaattgagct gagttcccgt accaggtttc
    15781 tcctgttcag tgaatactgc acaagttctt tcacaatttc ctcaccggca atcaggttca
    15841 caagcgaaat gtatttcacc cttattacca tccaggcaat aagcattgat aataagtcgc
    15901 ccctgaagca tactacctgg ggaacatcaa aaagggcagt ctcaagcgtg gcggtacctg
    15961 atgccaccag agcggcatct gaaatatgaa ggatctcata ggtcctgtcc ctgatcacct
    16021 gcagatccaa actgccagcc acatcggaat acagcgtctc gggcagattc ttaactcctg
    16081 caacaataaa ccggtagtct ttaaagtcag gaatcacttc cagcattttc gggaggatat
    16141 gtttgacctc acttttcctg cttccggcca gcagggcaat aactggcctg tcatccaggc
    16201 caagagatct cttgcacttg tctttatccg gtaatgaggt cagtttgacc tccgtttcat
    16261 caagcagcgg attcccgcaa tatatggccc ggatattatg cttgtcataa aaagtctctt
    16321 cgaacgggaa aatgatatac atcctgtcaa cgtacttttt cacttttttg acccgctttt
    16381 cattccaggc ccataatttg ggcgatatat agtaaaaaac cctgaagccg cttttttttg
    16441 cccactcagc gatccggaga ttaaagccgg gatagtcaat caatatcaac agatcgggtc
    16501 ctgatgaaat aatctgactt ttgcacaatg agatattctt tct
//
LOCUS       DAOOYG010000056        16491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3441_length_16491_cov_6.180092, whole genome shotgun sequence.
ACCESSION   DAOOYG010000056 DAOOYG010000000
VERSION     DAOOYG010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16491
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3441_length_16491_cov_6.180092"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..4159
                     /locus_tag="PK288_06475"
     CDS             392..4159
                     /locus_tag="PK288_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPM18602.1"
                     /translation="MYLETRTVLKSLIFLLFVSSPVYSQTGKSHKYQFKGDLQETSIL
                     PGRNSIVINYSLSELDIYSITNENGSFYRISIPGHNLSTDPGKPELPVFQRLITAPEG
                     SMPRIRISNVRSTRIRPSEKEIKGILMPAQYGESKKANTGERREFIMDKKAYASAGTL
                     QSDTVRIEHLGKIRERKLSNLIISPVKYNPAKNSIEIITSMRIEVSFSGESSGISKVS
                     DYESVAFNQVLEKGVLNYYPDNYITGYSTKPVGMIIVSDTAFRKHLKPLVLWKTQKGF
                     RVTVIYTGDAGNSAEEIKSSIALAYNSLRASGNSPEYLLIIGDVEKVPAFGSVNISDM
                     YYGEFDGGGDYIPDLFIGRIPAADTTEVKSVINKIIQYEKFGFAESNKFYSKALALAG
                     KDENYASYMNGQIKYAVTNYLKPENRIEPYPFYYPEGHTRKDSLIALINRGLSFINYT
                     GHGVKSGWLHLEFKSDDIRKLKNEGMYPFVISNACRTAQFNDSTSLGNKFVLATNKGA
                     IGFIGCSNDSYWDEDFNWAVGAGMPGSDPKYNETGLGAYDRLFHTHGEAPSEWYTTMG
                     QVNFAGNLAVSSTSSLRKKYYWETYSLIGDPSLTPYIGTPGRFNISIPDTLPVGIRSL
                     AITGEPFSYMAISRRGQLLDASFLSPSGSAVLDFQGIHNDSCLVVLTGQNKIPLIKTI
                     YFSSIAGEFLNLSKISLIDSLGNNNQKADYGETVFPNFTISNLGESDATNLTARITSS
                     SPWVTIVKGNAVIGTLRGKSEIELRDKLEIKLAEEVPDQGIITLDLILRDDRGEKRFK
                     TDITIHAPVLEIVNCLIDDSEEGNNNSVADPGETFNLVFQVRNYGSSDVSGYFGIRNF
                     NSALTVPDSNIKSGVLQFGKNSLITVRVKLSETAMFGDYISLLSTLDCTPYIVNRDFT
                     LRVGRVRESFESSGFDVFPWINFDPVPWQVTRTDSYDGNMSARSGRIGHNGATILRIR
                     TLYPEDDSLAFYYKVSSELNYDYLQFKLNGSEMLKASGETAWLRKAVKVPAGINDMEW
                     IYKKDNSVSQGADCAWVDLIDFSGTSPLKYVKRDLDLARIISPYQKDLLGLEMVSVRL
                     LNQGRDTLSGVSLAYQVNKEAPVHQFFPVILYPNQDSVTVTFDTRADLDRNGLYEIVV
                     YAYGDGDDYLLNDTLRISIINNELEEDVVVYPNPFGERLYVSVSSNMTGRITISLTDL
                     AGKNVMTSFHEISAGENLIELPIPKLGTSMYFLSVRGKRIYKVIPVIRMEQ"
     gene            4200..4781
                     /locus_tag="PK288_06480"
     CDS             4200..4781
                     /locus_tag="PK288_06480"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM18603.1"
                     /translation="MTNKQIPTREQALALLKKYNKTDSLLRHAYAVEGVMRHMAAKAG
                     EDVERWGIIGLIHDLDYEMYPDQHCRMTEKILRENNWPDDYIRAALSHGWGIVTDVEP
                     LSQLERTLYAIDELTGLVATSALVRPSRSVLDMEVRSVKKKWNDRRFAAGVDRKIIEK
                     GAAMLGISLDELINDCILGMREVAEETGLKGSM"
     gene            complement(4835..5581)
                     /locus_tag="PK288_06485"
     CDS             complement(4835..5581)
                     /locus_tag="PK288_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPM18604.1"
                     /translation="MRLLNIFIAAVLVFGLSTSCGSKGKKAETTQKAAAEAEGWTVLF
                     DGTSTAGWRGYNKPSFPEKGWEVVDGTLHCIGSGAGEAGGSGGDIIYDKKFGNFELSL
                     EWKISQGGNSGIFILAQEIPGEPIYKSSPEMQILDNDRHPDAKLGVNGNRMAGSLYDL
                     IPAVPQNTKPAGEWNQVGILCYQGTVVFKSNGVNVVEFHLWTDEWKKMVANSKFKDWE
                     WFINPAREGYIGLQDHGNDVWFRNIKIKEM"
     gene            complement(5613..7103)
                     /locus_tag="PK288_06490"
     CDS             complement(5613..7103)
                     /locus_tag="PK288_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18605.1"
                     /translation="MKENSKNITRRDFIGKTGSLAAGLTILPSTVISGFGYRAPSDKL
                     NIAIVGIGGMGNSNLKNVAPTENIVALCDVDWGYSKKVFEANPKAKAYWDWRKMYDEM
                     GKSIDAVMVATADHTHAIISAHAITMGKHVYLQKPLTHSVYESRLLTRLAAKYKVATQ
                     MGNQGSSDEGVSQTIEWLQAGEIGDVRKVEAFTDRPIWPQGLNTPKGEWVPETLNWDL
                     FVGPAKMRPYNSLYTPWNWRGWWDFGTGALGDMACHVLHPVFKGLKLQYPIRVQGSST
                     LLLTDCAPNAQMVTLVYPERVAPRNAKMKLPQVEVIWYDGGLQPPKPAGWPAGKNMND
                     AGGGVIFHGTKDKLICGCYGKDPWLLSGRKPVVPKTERRIENAMRGGHEMDWVRACKE
                     SPENRVPTKSDFAEAGPFNEMVVMGVLAVRLQGLNKELEWNGEKMEFTNIGREENVRL
                     TISDNFEIIDGDPRFNRPNITINAWDFATEMVKHTYRSGWSLPDMP"
     gene            complement(7213..7983)
                     /locus_tag="PK288_06495"
     CDS             complement(7213..7983)
                     /locus_tag="PK288_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPM18606.1"
                     /translation="MKKVYLSGILLLLGITVMAQTGQVPSGPAPMKPEMTEFYEPEVR
                     VVQPATILGMAPSDAIILFDGNDINREWEDSEGKPTRWVVRDGALVCVKGAGIIRTKR
                     KFNDFQLHIEWRTPAEVVGDGQGRGNSGVFLQGLYEVQVLDSYNNRTYRQGQAGSIYK
                     QYAPLVNASRKPGEWQTYDIIYTAPRFSSDSTQYFTPPRVTVLHNGVLIQNNVSLRGP
                     TLYIGIPEYSIRKHGAEPLVLQDHGDPVAFRNIWIREL"
     gene            complement(8400..9953)
                     /locus_tag="PK288_06500"
     CDS             complement(8400..9953)
                     /locus_tag="PK288_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPM18607.1"
                     /translation="MKIKYLLSTLFALLFVISSCDRDFEKINTNPILATDLDPLYLFT
                     EAQRVADVSTYHYQGEIVQQIVTPYGGVLEGGNRNTVNDANSNAMFNTLYQSPVRNLV
                     DALRKLEGNPARTNLYNMCRIWKAYCFQLLVDTYGDVPYTEAGLGFINANFLPKYDDQ
                     KAIYEDILKEYEQATDALDPAKDVVKEMFFNGDIAKWKKLGNSLLLRAGMRYTKLNPD
                     KAKQIVEKAVNPSRGGVMSSNADNVYLQYNPTYSYGTGNMLNSTERHNYYVGEPFVDY
                     LKSTNDPRARYILIKYEFPYNPLETAGATNNNLEDQQGMPYGYDENSISSAPGFPGKI
                     GAAFAYSQYNRATVGKIDGRSWLVTYALTELLLAEACHRDFITTGTALSHYEAGIRAA
                     MEQNNNLFGPAYAITPDEINDYLAQTEIAFDPAKALKQINEQYWVSSCMNWAENWANF
                     RRSGYPQLSPINYRGEDPSVQTPSAGGFIHRLPYPLREKTVNTANVNAAATNIGGDNL
                     GTRVFWDVQ"
     gene            complement(9975..13136)
                     /locus_tag="PK288_06505"
     CDS             complement(9975..13136)
                     /locus_tag="PK288_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM18608.1"
                     /translation="MKKKPNRMMQIHPVFKKLRHGIMATVVITCISVLFSVIDQAYAL
                     ASDPQQTVITGRVTDSRGEALPGVNVLLKGTTTGTITDTQGRYSLSVPDLTGTLIFSF
                     IGYTPLETPIAGRRSVDAQMTEETTALGEVVVTALGIKREAKSLGYSATAVDAREFAA
                     TKLTNMGNSLIGKVAGLNVSTPPTGPGGSSKIRIRGQSSFGGNNSPLFVVNGVPIDNS
                     YMTGSYNVDLGDGLLSLNADDIESMTVLKGATAAALYGYRAKDGVIIITTKSGAGQKG
                     IGVELSSSFQADRALDYTDFQYEYGQGENGIRPASLVDARRTGVWSFGTKFDGLPAWQ
                     YDGTEKPYLPFKDRIKAFYQTGLSFINSAAISGGDSRGGFRLSFTNHNSSSIVPTARF
                     KKNVVDLGLNYNLTKKLSVQMNANYSLENNHNPPAVGGQDFTTANTIYTLANSVDPRW
                     LEATYKDPVTGNEIALSRFTNRTNPYWTMYERFEDLKRNRVIGNILLRYQFTPWLYAQ
                     GRIGQDWYSMYHEFNMPTGTASLASPVTGFNGSYTQNFRDRRETNMDFLVGANRKLGD
                     FAVGATFGGNTMDNTYQMLQESVTNFYVRGLYTIGNGQTKNPSYSYSRKKVNSLYGTL
                     DLSFRDYLFVNLTGRNDWFSTLNPKSNNYLYPSASTSFIFSQALADLMPSWLDYGKIR
                     VAYAEVGGDTSPYTNALFYSMNTNPFNGYAIGGISGSVSPNPNLKPLKVKEAEAGLEM
                     IFFDRRISFDLAVYRKNTVDEILNVDISNASGFSSTRVNVGRLRNQGVEILFSVVPVR
                     TVNFSWESAFNYTYNDSKVLELAGGQQKIDVGEGDFIGVTSHEVGKPMASIRGIDYKR
                     DEKGRVITSDGRFLAGDYKTFGSAIPKHIGAWINTLTYRMFFVKAQIDFKSGHKMLSN
                     TNFNCLRHGLTKSSLVGREGGVVFDGYNSDGSPNTTPVEAEAFYADYRGKFVVTPFIY
                     DASFIKFRTLSAGADLTNILKLYFIKGITVSGYINNFFIISKHVDNLDPETVYSVSDN
                     KAGLESSGLPTLRSYGLSLNVKF"
     gene            13810..14295
                     /locus_tag="PK288_06510"
     CDS             13810..14295
                     /locus_tag="PK288_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18609.1"
                     /translation="MKKFFLDPGETGKIIRGFQFFFGIICVVVAIAWLILYPDPVKSG
                     ISFWVSFLFLSLFGIYQINAGAGRGRRFIGVGDKSLSLKRIALLPPRSINYDEIIKIE
                     IFPLSIVLILKSGRHITIRLGTTYTDIISPLKDAVAIRAEEMKIPVEFREAENHNSSS
                     G"
     gene            complement(14274..>16491)
                     /locus_tag="PK288_06515"
     CDS             complement(14274..>16491)
                     /locus_tag="PK288_06515"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HPM18610.1"
                     /translation="PVGNGRMGAMVFGDPGNECIQINEESIWAGSKINNNNPAALKNL
                     QHLREAIFRGDIRSAEKTAADNFVGTPPRIRSYQPLGNLLISYSWDSPVVDYNRRLDL
                     RNGIVSTSFTVGGHAFRQEVFASAVDDVIAFRIRPLKRGKINATLELHRERDAVIKLS
                     EDGTIVLSGQINDVPDSLSGPGGMHMKFAGETRLRIRKGKLAVEGSRLKISGAGEIMA
                     LFTAATDYNPAILDFDRKIDPEFVCRTILDRAGMKSWNSLIDAHIREHRKMFDRVSID
                     FGPDSLMVLPVDERLERLRSGKTDNGLIALYFQYGRYLLMSSSRHPGNLPANLQGIWN
                     KEYNAPWNSDFHTNINLQMNYWPAEVCNLSETALVLANFMKRITWPGSVTAREMYGAG
                     GWTMHHLTDPFGRTGVADGLWGLTPTSGPWMTFPLYEHFLFTRDTNYLRNIAYPVLKG
                     SAEFVLDFLVTSPEGFLVTCPSTSPENRYYLPGKKEPAYLTYAATIDIQTVRAVLDYC
                     SEAAQITEQDRELVERIKSAAGKLPPVLVSSKGTIQEWIKDYEEVEPGHRHMSHLLGL
                     YPLAQITPATMVLYEAAGKTIDRRLSYGGGHTGWSRAWIISFYARLHEGEKAYSHVIS
                     LLEKSTLPNLFDSHPPFQIDGNFGGTAGIAEMILQSHQGFIELLPALPDAWQEGNVKG
                     LVARGNFVVDLSWKNHKITSARIHSPTGGYCRIKYNDRLIGFNCEAGKDYYPLEEL"
BASE COUNT         4403 a         3779 c         3647 g         4662 t
ORIGIN      
        1 tgggttacca gaaccaggtc tgcagcaggc agcccttcat agttaccgga actgctaacg
       61 ggatcaatgt gaattacaaa accctcatgc cggaacataa gcgaaccatg gccgatgaag
      121 aacatttcaa catcgccggc tgaagtatga attctgtcaa cttcaggcat cttctgggaa
      181 aaagcggcag ctgtcattgt aaagtaaaat attgttacac ttattaatat cagaacccgt
      241 gatttcattt ttttcatttt gcaatataaa tccataatac aaatatacat gaacctttta
      301 cattatcttt gtgtttaatt cggggtagag attgaaattt ttatttgtat gcaaactgaa
      361 tcagggctgt agtgttaatt aattaacatt aatgtatttg gagacgcgca cagtattaaa
      421 gtcactaatt tttctcctgt ttgtatcctc accggtctat agtcaaacag gaaaatctca
      481 caaataccag ttcaagggtg atttgcagga aaccagtatt ctgcccggaa gaaattcaat
      541 cgttataaat tattcattat cagagctgga tatttattcc ataacaaatg agaatggcag
      601 tttttaccgg atctcaatcc ccggacataa tttgagtacc gatccgggca agcctgaatt
      661 acctgttttt caaaggttga tcacagctcc ggaaggcagt atgccgcgca tccggatcag
      721 caatgtccgt tcaaccagga tcaggccttc ggaaaaagaa attaaaggta ttctgatgcc
      781 agcccaatac ggggaatcaa aaaaagcaaa cacgggagaa aggcgggaat ttataatgga
      841 caaaaaggcc tatgcatctg ccggaacatt gcaatccgat acggtcagga ttgaacatct
      901 tggcaaaata agggaaagga aattatcaaa tctcatcatt tcgccggtaa agtataaccc
      961 ggcaaagaat agcattgaaa taatcacttc gatgaggatt gaagtcagtt tctcaggtga
     1021 gtcatcaggt atttcaaagg tttctgacta tgaatcagta gcttttaatc aggtacttga
     1081 aaaaggcgtt cttaattact atcccgataa ttatattacc ggttattcaa ccaaaccggt
     1141 aggcatgatt atagtaagcg atactgcttt caggaaacat ctgaagcctc ttgttttatg
     1201 gaaaacccag aaaggattca gggtcacggt aatctatacc ggagatgcgg gtaacagtgc
     1261 tgaggaaata aagagttcaa tagctttagc ttacaattcg ctcagggctt caggaaattc
     1321 accggaatat cttcttataa tcggcgatgt tgaaaaggtt cctgcgtttg gttctgtcaa
     1381 tataagcgac atgtattacg gggaatttga cggtggaggc gattatattc ccgacctgtt
     1441 tataggcagg atacctgctg ccgacacaac ggaagtaaag tcggtcataa acaagattat
     1501 tcagtatgaa aaattcggat ttgccgaatc aaataagttt tattcaaaag cactggcact
     1561 ggcagggaaa gatgagaatt atgccagtta catgaacggt cagatcaagt atgcagtgac
     1621 aaattatctg aaacctgaaa acaggattga accttaccct ttttattatc ccgaaggaca
     1681 tacaaggaaa gattcattga tagcacttat aaacagagga ttatctttta ttaattatac
     1741 cggacacgga gtcaagtcgg gatggctcca tctggaattt aaatcggatg acatcaggaa
     1801 actgaagaat gagggtatgt atccttttgt aataagcaat gcctgccgga ccgctcagtt
     1861 taatgattca acttcgttgg gcaacaaatt tgtgcttgca acaaacaagg gggcaattgg
     1921 ttttataggt tgctccaacg actcttactg ggatgaggac tttaactggg ctgtaggcgc
     1981 aggcatgccg ggttcagatc cgaaatacaa cgaaacaggt ttgggagctt acgacaggct
     2041 tttccacact catggagaag ctccgtctga atggtacaca acaatgggcc aggtcaattt
     2101 cgccggcaac ctggcagtca gctcaacttc ttccctgcgc aaaaagtatt actgggaaac
     2161 ctattcatta ataggagatc ccagccttac cccatatatc ggaactcccg gcagattcaa
     2221 catttcaatc cccgataccc tgccagttgg aatacggtca cttgcaataa cgggtgagcc
     2281 tttttcatac atggcaatat cccggagggg ccagctgctc gatgcctcct ttttaagtcc
     2341 gtccggttct gcagtattgg atttccaggg tattcacaac gattcctgcc ttgtggtcct
     2401 tacaggtcag aataaaatcc cgctgatcaa aacaatatac ttctccagca ttgccggaga
     2461 attcttaaat ctgagcaaaa tctctttaat agacagttta ggcaacaata accaaaaagc
     2521 ggattacgga gaaactgttt tcccgaattt tacaataagc aacctgggtg aatccgatgc
     2581 cactaatctt actgcacgca ttacttcctc atcaccctgg gttacaattg tgaaagggaa
     2641 cgcagtgatc ggaacactaa ggggaaaatc ggagatcgag cttcgcgata agcttgaaat
     2701 aaagttagca gaagaagtac ctgaccaggg aattataact ctcgatctta ttctcaggga
     2761 tgacaggggt gagaaaagat ttaaaacaga cataaccata catgctccgg tccttgaaat
     2821 tgttaattgc ctgatcgatg attccgaaga aggaaacaat aactcagtcg ccgatcccgg
     2881 tgaaaccttt aacctggtat ttcaggtcag gaattatggc agcagtgacg tttcaggata
     2941 tttcgggatc aggaacttca attctgccct gaccgttcca gattccaaca taaaatcagg
     3001 agtccttcag ttcggtaaaa attcacttat caccgtgagg gttaaactat ctgaaacagc
     3061 aatgttcggg gactatattt ctcttctttc aacactcgat tgtaccccat acatagttaa
     3121 cagggatttt accctaaggg tgggcagggt aagggagagt ttcgaatcat ccggcttcga
     3181 tgtttttccc tggatcaact ttgatccggt gccatggcag gtcacccgta ccgactccta
     3241 cgacgggaat atgtctgcaa gatcggggag aataggtcac aatggagcta caatcctaag
     3301 aatcagaaca ttgtatcccg aagatgattc cctggcattc tactacaagg tgtcgtcaga
     3361 gctgaactat gattatttgc agttcaaact gaatggttcg gagatgttaa aggcatcagg
     3421 tgaaactgcc tggctgagga aagccgtaaa agttcctgca ggaataaatg atatggaatg
     3481 gatttataaa aaggacaact cagtttcgca gggagctgac tgtgcatggg tagatctgat
     3541 tgatttttca gggacaagcc cgcttaaata tgttaaaagg gatttggacc ttgccagaat
     3601 tattagcccc tatcagaagg atcttttggg cctggaaatg gtatctgtca ggcttcttaa
     3661 ccagggtagg gatacattgt caggggtcag tcttgcctac caggtaaaca aggaggcacc
     3721 agttcatcag ttttttcctg tgatacttta tcccaaccag gattcggtga ccgtgacttt
     3781 tgataccagg gctgatcttg acaggaacgg tttgtatgaa attgttgttt acgcatatgg
     3841 cgacggcgac gattacctgt taaacgatac tctcagaatc agtatcataa acaacgaact
     3901 tgaagaagat gtcgttgtct atccaaatcc tttcggcgaa agattgtatg tttccgtttc
     3961 atccaatatg acaggcagga ttaccatcag tttaactgat cttgccggaa aaaacgttat
     4021 gacctcattt cacgagatta gtgcaggtga gaatcttatt gaacttccaa taccgaaact
     4081 gggtacttca atgtattttc tgtcggttcg tggaaaacgg atctataagg tgatacctgt
     4141 gatcaggatg gaacaatgaa ttgtcgggat aatagtactc caaaatacgc agaacattta
     4201 tgacgaataa acaaattccg accagggagc aggctctcgc attgctgaaa aaatacaata
     4261 aaactgacag tcttctcagg catgcgtatg cggtggaggg tgtaatgcgt cacatggcag
     4321 cgaaagcagg ggaagatgtt gaaaggtggg ggattattgg tctgattcat gacctggatt
     4381 atgaaatgta tcctgatcag cactgcagga tgaccgaaaa gatattacgg gagaacaact
     4441 ggcctgatga ctatataagg gctgctttaa gccatggctg gggaattgta accgacgttg
     4501 aaccgctttc gcaactggaa agaacgctgt atgccattga tgagctgacg ggtcttgtag
     4561 cgacaagcgc cctggtcagg ccttccagga gtgttctgga tatggaagtc agatcggtta
     4621 agaaaaaatg gaacgacagg aggttcgctg ccggtgtcga caggaaaatc atcgaaaagg
     4681 gtgccgcaat gctcggaata tcacttgatg aactgataaa tgattgtata cttgggatga
     4741 gagaggttgc agaagaaaca gggctgaagg ggagcatgta aaaaaagcgg gaacaccgtt
     4801 aatggtgttc ccgcatcccg gaagcccggt actcttacat ttccttgatt tttatattcc
     4861 tgaaccagac atcgttccca tgatcctgca gacctatgta tccttccctg gccggattta
     4921 tgaaccactc ccaatccttg aacttggaat ttgcaaccat ttttttccat tcgtctgtcc
     4981 agagatggaa ttcaacaaca ttaaccccgt tcgatttaaa aacaacagtt ccctggtaac
     5041 atagtattcc cacctggttc cattcgccgg caggctttgt attctggggt accgcgggaa
     5101 tgagatcata taatgatcct gccattctgt taccgtttac tccaagtttt gcgtcaggat
     5161 gcctgtcgtt gtccaggatc tgcatctcgg gagacgattt gtagattggt tcaccgggta
     5221 tttcctgtgc caggatgaaa ataccgctgt tccctccctg ggagattttc cattcaagcg
     5281 acaattcgaa gttgccgaat tttttgtcat aaattatgtc acctccggat ccgcctgcct
     5341 caccggcgcc ggatccgata caatgtagtg ttccgtcgac aacctcccat cccttttcgg
     5401 ggaatgatgg tttattgtat ccgcgccagc cggcagtgga tgttccgtca aagagaactg
     5461 tccatccttc tgcttcagcg gctgccttct gggtggtctc agcctttttt ccttttgacc
     5521 cgcatgatgt tgaaagtccg aaaaccaaaa cagctgcaat aaaaatgttt aacaatctca
     5581 tattttataa tttaatgatt aaaaatacaa tttcagggca tatcaggaag tgaccatccg
     5641 gaacggtaag tatgcttgac catttccgtg gcaaaatccc atgcattaat tgtaatatta
     5701 ggccggttga atctggggtc accgtcaatt atttcaaaat tgtcggatat tgtaagcctg
     5761 acattctctt cccgtccgat atttgtgaat tccatctttt ccccgttcca ttccagctcc
     5821 ttattgaggc cctgaagtct tacggccagt acccccatta caaccatttc gttgaatggt
     5881 cctgcttccg caaagtcaga ttttgtcggg acgcggtttt ccggactttc tttgcaggcc
     5941 cttacccagt ccatttcgtg tccgcccctc atggcatttt ctattcttct ttccgttttt
     6001 ggaacaaccg gtttgcgacc cgagagcagc catggatctt ttccataaca gccgcagata
     6061 agcttgtcct tcgtcccgtg gaaaattact ccgccgcctg catcattcat gtttttcccg
     6121 gcaggccatc cggcaggttt aggtggctgg agtccgccat cataccaaat gacttctacc
     6181 tgggggagtt tcattttggc atttcgcggt gcaactcttt caggatacac cagtgtgacc
     6241 atctgtgcat ttggagcaca atcagtcagc agcagggtgg aactgccctg aaccctgatc
     6301 ggatactgga gtttcagtcc cttgaaaacc ggatgaagaa catggcatgc catatcgccc
     6361 agagcgccag taccgaaatc ccaccagcca cgccagttcc agggggtgta gaggctgttg
     6421 tagggacgca tttttgcagg accgacaaag agatcccagt ttaaggtttc cgggacccat
     6481 tcacctttcg gagtattcaa tccctgcggc cagatgggcc tgtcagtgaa agcttccact
     6541 tttctcacat cgccgatctc tccggcctga agccattcta ttgtctggct cacgccttca
     6601 tcagaagaac cctggttacc catttgggtt gccaccttat actttgcagc aagcctggtc
     6661 agtaatctcg actcataaac agagtgggta agcggtttct gcaaataaac gtgcttaccc
     6721 attgttattg catgtgctga aataatggca tgtgtgtgat cggcggtggc aaccatcact
     6781 gcatcgatcg acttacccat ttcatcgtac atcttccgcc aatcccagta tgcttttgcc
     6841 tttggattgg cttcaaagac cttctttgaa tatccccagt caacatcaca cagagctaca
     6901 atattctcgg taggtgcaac attcttaaga tttgagttgc ccatccctcc tattcctact
     6961 attgcgatgt taagcttgtc acttggagcg cggtatccga aaccacttat aacagtgctg
     7021 ggaagtattg taaggccagc tgcaagtgaa ccggtcttcc caataaaatc gcgtcgcgta
     7081 atgtttttag aattttcttt cattgtaagg ttttttaaag ttttaagagg aattgaaaga
     7141 catgtaaaat tagtaatttc tgaattaaaa tcattattaa tcatttaggg attcaaatta
     7201 aaaccggcat cttcacagtt ccctgatcca tatattcctg aaggctaccg gatcaccatg
     7261 atcctgaaga acaaggggtt ccgctccatg cttccggatg gaatattccg ggataccaat
     7321 gtataaagta ggtccccgca agctgacatt gttctgaatc aggacaccat tatgtaatac
     7381 cgttacccgc ggcggtgtga aatattgagt actgtccgat gaaaaccggg gagcagtata
     7441 tataatatca taggtctgcc attcgcctgg ttttctgctg gcatttacca ggggggcata
     7501 ttgtttgtaa atactgcctg cctggccctg tctgtatgtt cggttattat aactgtcaag
     7561 gacctgaacc tcgtagaggc cctgcaggaa aactccgctg ttacccctgc cctgcccgtc
     7621 tccaacaact tcagcgggtg tcctccattc aatatgaagc tggaaatcat tgaatttcct
     7681 ttttgtccgg attatgcctg ctcctttcac gcaaaccagg gctccatccc tgacaaccca
     7741 tctggtcggt tttccctcac tgtcctccca ctcgcggtta atatcattcc cgtcgaacaa
     7801 aataatcgca tctgaaggcg ccatacccaa aattgtcgcg ggctgtacaa ctcttacttc
     7861 cggctcatag aactctgtca tttccggttt catgggagcc ggcccggaag gtacttgtcc
     7921 ggtctgtgcc ataacagtaa tacccagcaa tagaaggatg ccggatagat agactttttt
     7981 catttcagta aattatttcg gttaaggtaa ataatgatag gaagataaat cattgtatgc
     8041 aaataactgg tgttcaacaa aaccaaatga cagcttacaa gaaggtatca ttgtcgaaga
     8101 aagaatccat gtccgtgtat tcaaaaatac gaatctttcg ttatttcaca gtcactttcg
     8161 atgttttgca tcttgaagtt ttggattttc ttcattttca atccggatcg aaatcatttc
     8221 cgccagcttc tgtacctcaa aaatgaaaag gccttatcag aagatcaatt acccgattcc
     8281 tgccatcaaa tcccggatga caaatgaaag caggcttaac gatcctctga caaggccgta
     8341 atataacaat ttcagcctta acttaggatt gctgcatttt tgccgtgaaa tctatattct
     8401 cactgaacat cccagaatac tcttgtacca aggttatcgc ctcctatgtt tgtcgcagcg
     8461 gcattaacat tggctgtatt tactgttttt tccctcaggg gatatggcag ccggtgaata
     8521 aacccgccgg cagacggagt ttgtaccgac ggatcttctc cccggtaatt tatcggcgac
     8581 agctggggat aaccgcttct cctgaaatta gcccagtttt ctgcccagtt catgcatgaa
     8641 gaaacccaat actgttcatt tatctgttta agcgccttgg caggatcaaa agcaatttca
     8701 gtctgggcca ggtaatcatt tatttcatca ggagtaattg cataggctgg tccgaagagg
     8761 ttgttgtttt gctccatggc cgccctgata ccggcttcat agtgactgag tgccgttccg
     8821 gttgtgataa aatcccggtg acatgcttcg gcaagcagca actccgtcag ggcatatgtt
     8881 accagccagc ttctgccgtc aatttttccg actgttgccc tgttatactg ggaataggca
     8941 aaagctgcgc ctatcttacc tggaaatccc ggtgcagatg aaatggaatt ttcatcataa
     9001 ccgtatggca tcccctgctg atcttcaaga ttattatttg ttgcacccgc tgtctcaaga
     9061 ggattgtagg ggaactcgta cttaatcaga atatatctgg ccctaggatc atttgtgctc
     9121 ttcagataat caacgaaagg ctcacccaca taataattat gcctttcggt actgttcagc
     9181 atgtttcccg ttccgtagga gtaagtggga ttatactgta aatacacatt atctgcattt
     9241 gacgacataa ctcctcctct tgacggatta actgcctttt caacaatttg tttggcttta
     9301 tcgggattca gttttgtgta tctcataccg gctcgcagca aaagggagtt gccaagtttc
     9361 ttccattttg caatatcgcc gttgaaaaac atttccttca caacatcctt ggccggatcc
     9421 agtgcatcag ttgcctgttc gtattcctta aggatatctt catagatagc tttctgatca
     9481 tcatatttcg gaaggaaatt ggcattgata aaacccagtc ctgcttctgt gtagggcaca
     9541 tcgccgtaag tatctaccag gagctgaaaa caatatgcct tccagatccg gcacatatta
     9601 taaaggttgg ttctggctgg attgccttcc agtttccgga gagcatcaac cagatttctc
     9661 accggacttt gatacaatgt gttgaacatt gcattggaat tggcatcatt caccgtattt
     9721 ctgtttcccc cttcgagaac tcctccataa ggagtaacga tctgctgtac aatttcaccc
     9781 tggtagtgat aggttgacac atccgcgacc cgctgagctt cggtaaataa atacagtgga
     9841 tcaagatcag tcgcaagaat cggatttgtg ttgattttct cgaaatccct gtcgcaactg
     9901 ctgattacaa aaagcagggc aaacagtgtc gaaagcaaat attttatttt cataatctgt
     9961 gcaattaggg taggttagaa cttaacattt aaacttaatc cgtagctgcg aagcgtcggg
    10021 aggccggaag attccagtcc ggctttatta tcagacacgg agtatactgt ttcaggatca
    10081 aggttgtcaa catgtttgct gataatgaag aaattgttga tataaccgct gactgttatc
    10141 cccttgataa aatagagttt caggatattt gtaaggtccg cgcccgcaga aagtgttctg
    10201 aacttgatga agctggcgtc atatatgaag ggggtcacaa cgaatttacc gcggtaatct
    10261 gcatagaaag cttctgcttc caccggggta gtgttaggtg aaccatcact gttataaccg
    10321 tcaaaaacaa ctcctccttc cctgccgaca agagatgatt tggtaagtcc gtgtctcagg
    10381 caattaaagt tggtattgga aagcatttta tgccccgatt tgaaatctat ttgtgccttc
    10441 acaaaaaaca tcctgtatgt taatgtattt atccaggcgc ctatatgctt tgggatggcg
    10501 cttccgaaag ttttgtagtc tcctgcaaga aatcttccgt cggaggttat tacccttccc
    10561 ttctcatccc gtttataatc gatacctctg attgatgcca ttggttttcc gacttcatga
    10621 gaggtaactc cgataaaatc accttcgccc acatctatct tttgctgtcc gccagccagc
    10681 tccagcacct tgctgtcgtt ataagtatag ttaaatgcgc tttcccatga gaaattcact
    10741 gtacgtacgg ggaccacact gaacagtatc tctaccccct ggttcctgag ccttcccaca
    10801 ttgactctgg ttgatgaaaa ccctgatgca tttgaaatat cgacattcag tatctcatcc
    10861 acagtgtttt tccggtacac tgcaaggtca aaagagatcc tgcgatcgaa aaagatcatt
    10921 tccaggcctg cttctgcttc tttgactttc agaggtttca ggtttggatt agggcttacg
    10981 gatccggaga ttccgccgat tgcatagcca ttgaaaggat tggtattcat ggaatagaaa
    11041 agtgcatttg tataggggct ggtatcgcca cccacttcag cataggccac tctgattttt
    11101 ccgtaatcaa gccatgaagg cattaaatcg gccagggcct ggctgaaaat aaagcttgtg
    11161 cttgcagagg gatacaggta attgttggat tttggattaa gggtagagaa ccagtcgttc
    11221 cggccggtca ggttcacaaa cagataatcc ctgaatgaaa ggtcgagggt tccgtaaagt
    11281 gagttgacct ttttccggct gtaactgtag ctcgggttct tcgtttgtcc gttgcctatg
    11341 gtatacaggc cgcgtacata aaagtttgtg acggactcct ggagcatctg ataggtgtta
    11401 tccattgtgt ttccgccaaa agtcgctcca actgcaaaat cgcccagttt cctgttggca
    11461 cctacaagaa aatccatatt ggtttcccgg cgatccctga aattttgtgt atacgaacca
    11521 ttaaatcctg taacgggaga ggcaagtgat gctgtgccgg tgggcatgtt gaattcatga
    11581 tacatcgagt accagtcctg tccgattctt ccctgtgcat aaagccaggg ggtaaactgg
    11641 tatctaagca atatattgcc aataaccctg ttccttttca ggtcttcaaa acgctcatac
    11701 attgtccagt agggattggt gcggtttgta aaccttgaaa gggcaatttc gtttccggtt
    11761 accggatcct tgtatgtagc ctcgagccat ctcggatcaa cactgtttgc cagggtatat
    11821 atggtatttg cagtggtaaa atcctgtccg cccaccgcag gagggttatg attgttttcc
    11881 agtgaatagt tggcattcat ctgaacggaa agctttttcg taagattgta gttcagaccg
    11941 agatcgacca cgttcttttt gaatcgtgca gtaggaacaa tgctcgaaga attgtgattg
    12001 gtgaaggaga gtctgaaccc gcctcttgag tcacctcccg atatggctgc tgaattgata
    12061 aagctgagac cagtctgata aaaagccttt atcctgtcct taaaggggag atatggtttt
    12121 tccgtaccat catactgcca tgctggcagt ccgtcgaact tagtaccgaa gctccatacg
    12181 cctgttcttc gtgcatcgac aaggctagcc ggtctgatac cgttttctcc ctgtccgtat
    12241 tcatactgga aatctgtata atccagtgcc cggtcagcct ggaaacttga tgaaagttca
    12301 acgccaatac ctttctgccc tgcacctgat tttgtggtaa ttatgattac cccgtctttg
    12361 gcgcgatacc cgtaaagggc tgcagcagtt gcccccttaa gtaccgtcat tgactcaata
    12421 tcatccgcat taaggctgag caatccgtca cccaggtcaa cgttgtagga gcctgtcata
    12481 taactattgt caataggtac cccgttgaca acaaaaagcg gcgaattatt tcccccaaag
    12541 gatgattgcc cccttatcct gatttttgat gagcccccgg ggcctgtggg aggtgttgaa
    12601 acattcagtc cggcaacctt gccaatcagg ctgtttccca tattggtcag cttagttgca
    12661 gcgaattccc gggcatcaac cgcagtggct gaatacccga gactcttggc ttcacgcttg
    12721 attcccagag cggtaactac aacctcaccc agggcggtgg tttcctcggt catttgtgca
    12781 tcaaccgaac gccttccggc gataggtgtt tcaagcggag tgtaaccgat aaaactgaag
    12841 atcagggttc ctgtcagatc ggggacagaa agggagtatc ttccctgggt atcggtgata
    12901 gtaccggtag tcgtgccttt caataggaca ttaactcccg gcaaggcctc tcccctgctg
    12961 tcagtgaccc tgcccgtgat cacggtctgc tgaggatcgc tggcaagtgc ataggcctga
    13021 tcaatcactg aaaacaagac gcttatgcaa gtaatcacaa ctgtcgccat gatgccatgg
    13081 cgcagttttt taaaaacagg atgaatctgc atcatccgat taggtttctt tttcatagtg
    13141 aggaaaattt aattagggtt taactcaggt agttttgaaa taataatatt ctcccggttt
    13201 tcttcagact tctttattgt caggggaaat gtatcttccg gtcatctttt tatcataatg
    13261 accggctttg ctaaactctg acaatatttt attgcattct cattactcaa ttaatatatc
    13321 ccctcgaatg cagtacaaca aatacttcag tcaatgaatc agcacatttg cttcctttcc
    13381 ggatcagccc acacaggcac cgccatccaa tcagtaatcc tgccgaaaaa gccggccggt
    13441 cattttacct ggacgagaca aacccggaaa tcaataagaa atttattatc attacagaca
    13501 ctatccatac aaatatatat aaaaaaatta aacaaaaaag tgctccaaat gcgatgcaaa
    13561 tactgtcgcc aaatttccca tgcccggtct gtacttatgc agtaagaatt aatctcctgt
    13621 tcagcagcag tcttttacgc aaggtttaat ttaccggcga aagggctttt aaatatttgc
    13681 catatattgc gtaaaaaaat cagggtatga aacccacatg atacaaaatc acaaacaagc
    13741 atgaaaataa gatctatgtg gtttctatgc gaaccattta atcttcagta gcttataaat
    13801 ttttatcata tgaaaaaatt ctttctcgat ccgggcgaga ctggaaaaat tattcgcggg
    13861 ttccagtttt ttttcgggat catatgtgtt gttgtagcta ttgcttggtt gattctgtac
    13921 cctgatccgg ttaaatccgg gatttccttc tgggtcagtt ttctgtttct gtcattattc
    13981 ggcatttacc agataaatgc cggtgctggc aggggcagga gatttatcgg ggtcggggat
    14041 aaaagcttat ctcttaagcg tattgcctta ttacccccac ggtcaataaa ctacgatgaa
    14101 attataaaaa ttgagatatt tcccctgagc attgtgttaa tcctgaaatc aggcaggcat
    14161 ataaccataa gactggggac aacatatacg gatattatca gtcccctcaa ggatgctgtt
    14221 gcgataaggg ctgaagagat gaagattcca gttgaattca gggaggcaga aaatcacaat
    14281 tcttccagtg gataataatc ttttccggct tcacaattaa acccgattaa ccggtcatta
    14341 tacttaatcc tgcaatagcc gcctgttggt gaatggatcc tggccgacgt gattttatga
    14401 tttttccagc ttaaatcaac aacaaaattt ccccttgcaa caaggccttt tacatttcct
    14461 tcctgccagg catccggcag ggcaggaagt aattcgataa agccctggtg actttgcagg
    14521 atcatttccg ctattccggc cgttcccccg aaattgccat caatctggaa cggcggatgg
    14581 ctgtcaaaaa ggtttggcag ggttgatttt tcaagaaggc tgattacgtg actgtaagct
    14641 ttctctccct catgcaggcg cgcatagaaa ctaattatcc aggccctgct ccaccctgtg
    14701 tggcctcctc cgtatgagag gcgtctgtcg attgttttcc cggcagcttc atacagaacc
    14761 atcgtggcag gggtgatctg ggccagcgga tacaatccca gtaaatggga catatgtctg
    14821 tgccctggct caacttcctc ataatccttt atccattcct gtatcgttcc ttttgaacta
    14881 accagaacgg gcggcagttt cccggctgca ctttttatcc gctccacaag ttcacgatcc
    14941 tgttccgtga tttgtgcagc ttcggaacag taatcaagca cagccctgac agtctggata
    15001 tcaatagtgg cagcatatgt aagatatgcc ggttcttttt tgccgggcag gtaatacctg
    15061 ttttcaggtg aagttgaagg gcatgtaacc aggaatcctt cgggagaagt cacaagaaag
    15121 tccagtacaa attctgctga tcccttaaga acaggatatg caatattcct gaggtaattc
    15181 gtatcgcggg taaataaaaa atgttcatag agagggaacg tcatccaggg tccgcttgtc
    15241 ggagtcaggc cccagagtcc gtcagcaact cccgtccgtc cgaacggatc ggtgagatgg
    15301 tgcattgtcc agcctccggc tccgtacatt tcccgggctg ttacgctgcc gggccaggta
    15361 atccttttca tgaaattagc aagcaccagt gccgtttcag acagattgca cacctccgcc
    15421 ggccagtaat tcatctgaag gttgatattg gtatgaaaat cggagttcca cggggcattg
    15481 tattccttgt tccagatgcc ctgcaggttt gcgggaagat tgcccggatg ccgtgatgag
    15541 ctcataagca ggtaacgtcc gtactggaaa taaagcgcga tgagcccgtt atccgtcttt
    15601 cctgaccgaa gccgttcaag gcgctcatct accggaagta ccatcagaga gtcggggcca
    15661 aaatcaatgc tgacccggtc gaacattttg cggtgttccc tgatatgggc atcaatgaga
    15721 ctgttccagg atttcattcc ggccctgtca agaatggttc tgcaaacaaa ctccggatca
    15781 atctttctgt caaaatcaag tattgcagga ttataatcag ttgcagctgt aaatagtgcc
    15841 ataatttcgc ctgcgcctga gatcttcagc ctgcttccct caactgctag tttacccttt
    15901 ctgattctca gccgggtttc ccctgcaaat ttcatatgca ttcctcccgg accgctcagg
    15961 gaatcgggga cgtcatttat ctgtcctgac aggactatgg tcccgtcctc tgacagcttt
    16021 atgacggcat ccctttccct gtgcagctca agcgtggcat ttatcttccc tctttttaac
    16081 ggtcgtatcc taaaggcgat tacatcatca accgctgagg caaaaacttc ctgccggaaa
    16141 gcatgtccgc cgacagtaaa tgaggttgaa acaatcccgt tacgaaggtc aaggcgtcgg
    16201 ttgtaatcca cgaccggtga atcccaggaa taactaatca gcaaattgcc cagtggctgg
    16261 tatgagcgta ttcgcggcgg ggttcccacg aaattgtctg ctgccgtttt ctcagcagat
    16321 ctgatatcgc ccctgaagat ggcttcacgc aaatgctgaa gatttttcag ggcagccgga
    16381 ttgttgttat tgatcttaga tcctgcccag atgctctcct catttatctg aatgcattca
    16441 tttccgggat ctccgaacac cattgcaccc atgcgcccgt tacccacagg c
//
LOCUS       DAOOYG010000057        15659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3710_length_15659_cov_7.811266, whole genome shotgun sequence.
ACCESSION   DAOOYG010000057 DAOOYG010000000
VERSION     DAOOYG010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15659
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3710_length_15659_cov_7.811266"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PK288_06520"
     CDS             complement(<1..230)
                     /locus_tag="PK288_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM18611.1"
                     /translation="MHLKGNPSKGLLGTTIGFFFGFAAVALYGPTAVKFREAMDLTPA
                     MVGLLIAIPSLSGSLLRIPFGAWVDITGGKIP"
     gene            530..1297
                     /locus_tag="PK288_06525"
     CDS             530..1297
                     /locus_tag="PK288_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPM18612.1"
                     /translation="MKIISWNVNGIRAAAKKSFFKDLEMMDPDIICLQETKAQDHQVA
                     ETLESVGHFHIFSNSAQRPGYSGTAVISKIKPLGISKDIDVSEHDTEGRVICLEFEQF
                     FLVNVYVPNSGAELARLTYRQEWDKAFFSYLKNLEKVKPLVICGDLNVAHREIDLARP
                     RENYNKTAGYMQEEIDGMDRLIEGGFADTFRHFYPDQAERYSWWSFRAGARRKNIGWR
                     IDYFLVSRAFLPKVKDAFILEEIGGSDHCPVGVILDI"
     gene            complement(1331..2500)
                     /locus_tag="PK288_06530"
     CDS             complement(1331..2500)
                     /locus_tag="PK288_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18613.1"
                     /translation="MQTNRRRFLKNSIGPATGLAALSMFPQGIIGQGINHASSPAEIK
                     VKPVKRTVPSGSIRFSVIGLNHGHIYEMTDAVIGGGGKLVSVYALEPELLSGFKKRYP
                     GVRIARSEEEILGDKTIQMVVSASIPVERAPLGIRVMKAGKDFMVDKPGIITMSQYDE
                     VRKVQKAENRIYSIMYGERLGSPAAVKAGELVMAGAIGKVVQTIGLGPHRMNPASRPA
                     WFFDPSRAGGILCDIGSHQCDQFLFYTGSPEAEVVVSQVGNFNTAGYPAYRDFGDMIV
                     RSPHATGYIRIDWFTPDGLATWGDGRTFILGTEGYIELRKYIDIAGRKGGNHLFLVNR
                     KETVYYDCSEVRTPYGEQLVNDVINRTETSMSQEHCFLAAWLALSAQEKAREIGG"
     gene            complement(2737..4404)
                     /locus_tag="PK288_06535"
     CDS             complement(2737..4404)
                     /locus_tag="PK288_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPM18614.1"
                     /translation="MKITVNNLQLELHEGARVKDAVLKYYSQSGKKSRVRNPVVEDGY
                     GNQVAGDGELTDGSVLFIRKGRGKDVSFPGLVSLLLIAGLAYSCSTGRKAPAGRESGR
                     QAVIFAVNDMHAAIDNFPRLAFIVDSLRGIYPDMLLVGAGDNQTGNPVNDQFPEKGLP
                     MIELMNATGFDLSAVGNHEFDSHQDGFSNLTHKARFSFICANLASDPALDILISPYKI
                     ITLPNGLRLAFLGLLQINQNGIPDSHPDNVKGFTFSSPFETAREFMWLKDQCDIFIPL
                     THIGFENDVKLAEAMPKGISLIIGGHSHTRIDKEQIHNGILITQAGSKLRYGTLIRLS
                     LKSDGTLDRSMKLISVSGSGSEKTSVRAMVDKFNDNPVLNEVIATAEDDLSSHQELGY
                     LMADAQRDAAKADISFVNPGGVRINHLVKGPITVMNVYQLDPFGNELVVTRLTGNEIR
                     SLMFAAFPLDDNSPLWPSGIRTELTMDGKGNLKEVVLLTENGTPFDMNKTYNVAMNSY
                     MTSVYRYDHADPGQSLFLTTAEATINYLKQSGSIRSYKGEKRVVVIR"
     gene            complement(4494..6155)
                     /locus_tag="PK288_06540"
     CDS             complement(4494..6155)
                     /locus_tag="PK288_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM18615.1"
                     /translation="MINKYLKQTEFGSYGEFCSGFEIDTPENFNFAWDVVDEIARVEP
                     EKTAMVWCDDKGNEAVFTFAQMKYYSDKAAGFFRSAGICKGDPVMLILKRHYAFWFCT
                     LALNRIGAITIPATHLLSAKDIIYRNNAAGIKMIVCVPDPLVMQHIEESETLSPTLKL
                     KAVIDRDSRGWLNFTRGMEESPEEFPKPAADEAPGNNDIMLLYFTSGTTGMPKMVTHD
                     FTYPLGHIVTAKYWQNVQENGLHFTIADTGWAKSAWGKIYGQWLAGSAVMTYDYDKFV
                     PRDILKVIEKYKVTTFCAPPTIYRFLIKEDFDGIDLGSLKYCVVAGEPLNPEVFRIFL
                     EKTGIPLMEGYGQTECTVAIATYPWMDPKPGSMGKPTPGYDIDIVDESGNSCEVGYEG
                     EIVIYTGKKKPSGMFRGYYRDAELTRTVWSDGVYRTGDMAWRDEDGYYWFVGRADDVI
                     KSSGYRIGPFEVESALIEHPAVLECAVTAVPDPDRGQIVKATVVLAGNYEPGEELAKI
                     LQDHVKRVTAPYKYPRIVEFVAELPKTISGKIRRVQIRENEGQAG"
     gene            complement(6179..6733)
                     /locus_tag="PK288_06545"
     CDS             complement(6179..6733)
                     /locus_tag="PK288_06545"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPM18616.1"
                     /translation="MTDQIRQVATRIKEIREISEVSPETLALKLGLSTDLYLKYESGE
                     ADIPVGIIFRISEMFNVDMSVLLGGDAPRLQIFSIVRKGKGLKLERRQQYRYESLAGH
                     FINKKAEVFMVTIDPKPEGSLPEFSSHPGQEFNYVLEGSMLTVIGGHEIVLNAGDAVY
                     FDSGYNHAMKALNNEQVRFLAMVL"
     gene            7098..8192
                     /locus_tag="PK288_06550"
     CDS             7098..8192
                     /locus_tag="PK288_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18617.1"
                     /translation="MSYFFRTFILFSKIQFVIKVVVIYDLNPSWDHTEIREVRNSARI
                     LFESLQLEGIKAYIEELNNPHLERILEKYNPDNTIIFNLCESLPGLQYSEKKIVEIIE
                     NHGFTYTGNTPEVIGLSYDKQKVKDVLSSMGIMVPRGAILDPEEAEGWTLFPAIVKPS
                     REHCSLTITEKSVVYDTSSLREQILLVNNELKQQAMVEDFIDGREFHVSVWNNEEPEM
                     LPPAEMDFSAFREAKERLCTYDSKFIPGSEHYEKIQTLVPAPLDGKQFRKLEKTALAA
                     WKGFGCRDYARFDFRLRNDEFYLLDINPNNDISVDTSFSMAAEIQNYSYSQMVKRIML
                     MAAERHPELAEEFQLNNLPARGHSRKSIVA"
     gene            8473..9717
                     /locus_tag="PK288_06555"
     CDS             8473..9717
                     /locus_tag="PK288_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPM18618.1"
                     /translation="MKTNVIQVLAIFAALIFAGSCSKSDSPEPDLTPANIKLTPVALQ
                     AIQSSNEFGIGLFTRVAEEENMNLMLSPLSASTVLTMLLNGCGENTYTQLKEMLNYPS
                     GMTIGDINEAYKSLVGQLLKADPDVKLSLANAIFYRNGFTVKPPFLAVMGNDYSATVG
                     GLDFDSPSALATINKWANDNTNGKIPKVLDEIDPDAVMFLMNALYFNGDWSYQFDKSL
                     TQDRPFYPDGSSTINVSTMNSEVGAKIVAGTGFRAIEMPYGRTNFTMVVIVPDKTLAG
                     FYTSFTPLAWSQLTGSLDAKPEFDKIKVYMPKFKFSYEKYLNSQLQSMGMVDAFIPYQ
                     ANLSGISDASIFVSFVKQNTFVEVDEEGTEAAAVTTIGVNITSMPPEPLQFVIDKSFI
                     FAIRERTTNTLLFIGQVVNPEN"
     gene            9997..11283
                     /locus_tag="PK288_06560"
     CDS             9997..11283
                     /locus_tag="PK288_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18619.1"
                     /translation="MKTFIRTSVTLLILAASLPACMDKTVERITYEANVPIYMPFNKF
                     RASFSKSEPVEITAPGKFYFKDNYLFVNEVGKGIHVIDNSNPANPIKIAFYEIMGNVD
                     MAIRGNILFADSYIDLVAIDISDIENPVETGRIKNIFPEVVPEGDIWYPYAMVDKSKG
                     VIVGWEVKTVTEEHEPGSKWDGWIYRKEMVMMGAADSRANWSAGAGTAGSMARFMLNG
                     RYLHLIAHPWMLKTVDVEDSRKMNIADSVNVSRNMETLFLLGQNMFVGTTTGMLIYDV
                     SDPVKPVLVSQYDHITACDPVVADGDYAYVTLRTGTRCANGQNLLEVIDISSITSPYL
                     VKSYPMYNPHGLGIDGNLLFICDGAAGLKIFDKSDPLDIINKKIAHYPDFLTFDVIPM
                     KGILMLVGEDGIYQYNYSDPTNIVQISHIPITGDKK"
     gene            11287..12033
                     /locus_tag="PK288_06565"
     CDS             11287..12033
                     /locus_tag="PK288_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18620.1"
                     /translation="MYGTRITAAIVLFFLTFTLNAQNRSKQTIVLNNGSRLTGTVLAD
                     SSGYLKLRITAPQVLTLEKSGIALIKPDLRTEKPVTDRHGYSIRLSASVLAGRNLDGN
                     IRDLSIHLSNGYQFRNGICAGFGSGIEKFDEVSVLPVYADLRYHLLKSRLSPFFWVKG
                     GISFTYDDLGDRQNYYYGYYSESKGGFMFNTGAGIELASWRRNAVNMGVGYRYQKITF
                     RQVNRWNEEVINELVSSFNRIELQFGLVFR"
     gene            12111..12698
                     /locus_tag="PK288_06570"
     CDS             12111..12698
                     /locus_tag="PK288_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM18621.1"
                     /translation="MRKSDKDEHTLSDLELIRRCSEGDPRAQELLYKRYFSYAMSVCI
                     RYTRNRYEAMEIVNDSFMKVLDNIREHDGSGSFRSWYGRILVNSAIDNYRRNARHNSH
                     LQISEIESTEDCEPEISAELSANEILSLFDLLPDHYRVTFNLYEIEGYSHEEIGQMLD
                     ISSSTSRSNLSRAKKMLRELYKRNFNPGNSSNEQV"
     gene            12685..13986
                     /locus_tag="PK288_06575"
     CDS             12685..13986
                     /locus_tag="PK288_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18622.1"
                     /translation="MNRFDDIFRDNVEKAFSSYNADHLAEEGWNSFVAGKAKRRRRTI
                     WFPLWARAASVMLVIGLGVFIAYKASIRHSAPEILSGPGSSSNLPAESPAEDKTPEPS
                     SASAARPSISGTTPVQDKTEKSPAWQQNSYEEKYSPSEFILTGAEPLPLPEISLDRFI
                     NRYGSPPPHVLAENELPEDKIPEDPVSAAVMPEGFVMDKNSSGGEKRHGTGILMAGVS
                     GLLAQSAGTASPSSGLSVGFYLDQKLSGKISVRPGLAIAMQSFGTGDVSSQDRFNYTM
                     SLNDGTKGIPHSSSGHLSMLAMEVPLNIVFRISGKERSGFYLSTGASTMIYLSQQFRA
                     GYVNKYTKSEINTMTGESFSETRYSTVEVENNYSAFSHTDFFGLANLSAGYSFAYGKT
                     GTMLVEPFLQLPVSDLTSLNLRVRYGGISMKLKFGRQERDN"
     gene            14194..15657
                     /locus_tag="PK288_06580"
     CDS             14194..15657
                     /locus_tag="PK288_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HPM18623.1"
                     /translation="MKKLFRALVIIFALLVLIVIIKTATFSSMQLKPEAIELPAFPDA
                     SVEHLSEAITYPTISEAIDSPVDTAAFKGFHKFLMEAYPLIHRDLKKEVFSSFSLLYT
                     WEGSDSRLKPVVLTAHLDVVPTGELSSWSEPPFSGKNDGTFIWGRGTLDDKSSMISIM
                     EAVEKLLSENYKPERTIYLAFGHDEEIGGLKGAGVMAGELVKRNLDAEFVLDEGYAVT
                     IGMVPMIKKPVALIGTSEKGYLSVSLSVEMEGGHSAFPARESAITLITGALNRIVSNQ
                     MKSGISEPVNDFIRYIGPEMPWYARAIFANKWLFKGLILKIYEGGNNSNALVRTTTAP
                     TIIHAGIKDNVIPTRAEAVVNFRILPGETSGDVINHLERVIADDRISIRPLEDLKEPA
                     PVSPIDAPGFDIIHKTIRQVYPEALVAPTMMLAASDSRKYLGISRNIYNFAPIIVTDE
                     DLARTHGLNERTQIGHFIRGVGFYYQLIRNCNNQSAG"
BASE COUNT         4340 a         3861 c         3464 g         3994 t
ORIGIN      
        1 aagggtatct tgcccccggt gatatctacc caggctccga agggtatccg gagcaatgac
       61 cctgacaacg acgggatggc aatcagcaag cctaccatgg ccggagtcag atccatggct
      121 tccctgaatt tcacggccgt tggcccgtaa agggccaccg ctgcaaatcc gaaaaaaaat
      181 ccgatggtgg tacccagtaa tccctttgaa gggttccctt tgagatgcat aatgtcagat
      241 tgaaatttta atattgcaat ttatccaaaa catgaaaaag gaataagaaa aaatgaaaat
      301 tatcattctg agatttgctt cagtgaaaag ataatccgct gcaaatgctt caaatgcttt
      361 tcaggccgga gatatttatc atacaataat gactattttt acaaaatccc aataaaaatc
      421 cgattatgaa acccacatga tataaaatca caaacaagca tgaaaataaa attcatgcag
      481 gttccatgtg aaccacttaa tcttcagtag tatataaaat tattttcaca tgaaaattat
      541 atcatggaat gtgaatggca tccgggccgc ggcaaagaaa tcgtttttta aagatcttga
      601 aatgatggat cccgacataa tctgtctcca ggaaaccaag gcacaggatc accaggttgc
      661 cgagacactt gaatctgtcg gtcattttca tatcttttcg aactccgccc aaaggccggg
      721 atattcaggg accgccgtca tctcgaaaat caagcccctt ggtatttcaa aagatatcga
      781 tgtcagcgaa catgatactg aaggccgtgt tatttgcctt gaatttgaac agttcttcct
      841 cgtgaatgtg tatgttccca attcgggtgc cgaacttgca agactgacct acagacagga
      901 atgggataag gcttttttca gctatctgaa gaatcttgaa aaggtcaaac cgctggtaat
      961 ctgcggcgac ctcaacgttg cccacaggga aatagacctg gcgcgcccaa gggaaaacta
     1021 caacaagact gccggctaca tgcaggaaga aattgacgga atggacaggc tgatagaagg
     1081 cggatttgcc gataccttcc gccatttcta tcccgatcag gcggaaagat actcatggtg
     1141 gagcttcagg gccggggcaa gacgaaagaa tatcggatgg agaatcgatt atttccttgt
     1201 cagcagggca ttccttccaa aggtgaagga tgcatttatc ctagaggaaa tcggcggatc
     1261 agatcattgt cccgttggag tgatactcga tatttaatat attataataa ggtatccccg
     1321 taaatcagaa tcatccccct atctcccttg ccttttcctg ggcactcagg gccagccagg
     1381 cggcaaggaa gcaatgttcc tgcgacattg acgtttcagt acggttgata acatcattca
     1441 ccagttgttc cccgtatggt gttcggactt ctgaacagtc ataataaact gtctctttcc
     1501 ggttcaccag gaaaagatgg ttgcctcctt tcctgcctgc gatatcaata tatttccgca
     1561 attctatata accttcggta cccagtatga atgtccttcc atcaccccat gttgcgagtc
     1621 cgtcgggggt gaaccagtca atgcggatat aacccgttgc atgcgggctt cttactatca
     1681 tatcgccaaa atcccggtaa gccggatagc cggcagtatt gaaattgccc acctgggaaa
     1741 cgactacctc agcttccggg gaacctgtat aaaagagaaa ctgatcgcac tggtgcgatc
     1801 caatgtcaca taatattccc ccggccctgg atggatcaaa gaaccaggca gggcgcgaag
     1861 cgggattcat ccggtgcggt cccagcccga tggtctggac tacttttcca atggcccctg
     1921 ccatgaccag ctcccctgcc ttgaccgcgg cggggcttcc cagccgctca ccgtacatta
     1981 tggagtaaat tcggttttct gccttctgaa ctttccttac ctcgtcatat tgactcattg
     2041 tgataattcc gggtttgtca accatgaaat cctttcctgc cttcataacc cttattccca
     2101 ggggtgccct ttcgaccggg atggaagcac tgaccaccat ctgaatcgtt ttatccccca
     2161 ggatctcttc ctcgctcctg gcaattctca caccgggata tcgctttttg aaaccggaaa
     2221 gcaattccgg ttccagcgca taaacagata ccagtttccc accgccgcca attactgcat
     2281 ccgtcatttc ataaatatgg ccgtgattga gccctattac cgaaaacctt attgatcctg
     2341 acggtacagt ccgtttaaca ggtttaacct ttatctcagc cggactggaa gcatgattta
     2401 ttccctgacc aataattcct tgcgggaaca ttgacaaagc tgccagccct gttgccggac
     2461 caatggagtt cttcaggaat ctgcgtcggt tggtttgcat gattgcaagt gtttaatcag
     2521 tagatgaaaa tatcaaactt aagaaaaatg attaaaaatg atctccggaa aaatcataca
     2581 atgcttcaaa ccattaatat cttccagtcc gatcatcagt ataccaacag gggaaaatga
     2641 cttccggaaa cggtatccat ccttgctgca gcacttccgt caggcaggaa aacggcctcg
     2701 taaaatcagc cggaacggga tcagcttcca ggctggttac ctgataacaa cgacccgttt
     2761 ttctccctta taacttctaa tactgcctga ttgcttcaga tagtttatgg tcgcctcagc
     2821 agtagtgaga aaaagcgact ggcccggatc agcatgatca tacctgtaaa cagatgtcat
     2881 gtaactgttc atggcaacgt tataggtctt attcatgtcg aacggagtac cgttttcagt
     2941 aagtagaaca acctctttca ggttaccctt gccatccatc gtgagttcag tcctgatccc
     3001 cgatggccag agcggtgaat tgtcatcaag gggaaatgcc gcaaacataa gggaacgaat
     3061 ctcattcccc gtaagcctcg ttacaacaag ttcatttccg aacggatcaa gctgataaac
     3121 attcataact gttatcggac cttttaccag gtgattgata cgaacacccc cggggttaac
     3181 aaacgaaata tctgcctttg cagcatcacg ctgggcatcg gccataaggt atcccagttc
     3241 ctggtgtgag gaaagatcat cttcagccgt cgcaatcact tcattgagaa ccgggttatc
     3301 attgaactta tcgaccattg cacgaacgga ggttttttca gatcccgatc ccgaaacact
     3361 gatcagcttc atgctgcggt ccagggtacc gtcacttttc agggataacc tgataagggt
     3421 accgtatctg agtttactcc ccgcctgggt aatcaggatc ccgttatgga tctgctcttt
     3481 gtcaatccgt gtatgcgaat gccccccgat aatgagactg attccctttg gcattgcttc
     3541 ggccagtttc acatcgttct caaagcctat atgggtcaat ggaataaaga tgtcacactg
     3601 atccttcagc cacataaatt cacgggcggt ttcgaaggga gaactgaatg tgaatccttt
     3661 aacattatca ggatgtgagt cgggaatacc gttctgattg atctgcagca gcccgagaaa
     3721 tgccagtctc agtccgttgg gcagggtgat gattttatag gggcttatca ggatgtccag
     3781 ggcgggatct gatgccaggt tggcacagat aaagctgaat cgcgccttgt gagtcaggtt
     3841 gctgaatccg tcctggtgtg aatcgaactc atgattcccc actgcactca gatcaaagcc
     3901 cgtggcattc atcagttcaa tcatcggcag tcccttttcg ggaaactggt cgttcacagg
     3961 attccctgtc tgattgtcac ctgcgccaac cagaagcata tcaggataaa taccccgcag
     4021 gctatcaacg ataaatgcca gtctggggaa attatcaatt gcagcgtgca tgtcgttaac
     4081 tgcaaagata acggcctgcc ttccggactc cctgcctgca ggggcttttc tgccggtgct
     4141 gcaggagtaa gcaagtccgg cgataagtag cagtgaaacg agtcccggga atgaaacatc
     4201 cttcccgcgc cctttcctga taaaaagcac gctgccgtca gtcagttccc cgtccccggc
     4261 aacctggttc ccgtatccgt cctccacaac aggattacgt acacggctct ttttccccga
     4321 ttgcgaataa tacttgagca cggcatcctt cacccgtgcc ccttcgtgaa gctcaagctg
     4381 taaattgttc acagtgattt tcatatgtta cggatttgaa aatgatacat agattttaat
     4441 taccaggcac atgaaatcga tcaatgctgc cggatcccgg cctgttttcc gggttatccg
     4501 gcttgtcctt cattttcacg gatctgtact cttctgatct tgccgctgat cgttttaggc
     4561 agttcagcga cgaattcaac tatcctcgga tacttatagg gagctgttac tctcttaaca
     4621 tgatcctgaa gtattttggc cagttcttca cccggttcat aattcccggc aaggaccacc
     4681 gttgccttaa ctatctgtcc cctgtcggga tcaggcaccg cggttaccgc acattcaagc
     4741 acggcaggat gctcaataag cgcactttcg acttcgaaag gaccgatcct gtagcccgaa
     4801 ctcttgatga catcatccgc cctgccgaca aaccagtaat atccgtcctc gtcgcgccag
     4861 gccatatcgc cggttctgta aactccgtcc gaccagacgg tcctggtcag ttctgcgtcc
     4921 cggtaataac ccctgaacat tcctgagggc tttttcttcc ctgtataaat gacaatctct
     4981 ccttcatacc ccacttcgca ggaattcccc gactcatcaa caatatcaat atcataaccg
     5041 ggggttggct ttcccattga accgggtttg ggatccatcc agggataggt ggctatggca
     5101 accgtacatt cagtctgtcc gtacccttcc atcagcggga tcccggtctt ttcaagaaag
     5161 atcctgaata cttcgggatt taacggctca ccggcaacaa cacagtattt cagactgccc
     5221 agatcaatac cgtcaaaatc ttcctttata agaaacctgt aaatggtcgg aggggcacag
     5281 aaggtagtta ccttgtattt ctcaataact ttaaggatat cccggggaac aaacttatca
     5341 taatcataag tcataaccgc actccctgcc agccactgac catatatctt accccaggct
     5401 gatttcgccc aaccggtgtc tgcaatggtg aaatggaggc cgttctcctg tacattctgc
     5461 cagtattttg ccgtaacaat gtgacccaga ggataagtga aatcatgtgt taccattttg
     5521 ggcataccgg ttgttcccga tgtaaaatag agaagcatga tatcattgtt gccaggcgct
     5581 tcatccgcag caggctttgg aaactcctcc ggactctctt ccattccccg cgtaaaattg
     5641 agccagcccc tgctgtccct gtcaatcacc gctttcagct tcagggtcgg ggacagggtt
     5701 tcagattcct caatatgctg cataacaagt ggatcaggta cgcaaacgat catttttatc
     5761 cctgcagcat tgttcctgta aattatgtct tttgcagaca aaaggtgagt ggcgggaatc
     5821 gtgattgccc ctatcctgtt cagggccagt gtgcagaacc agaaagcata atgcctttta
     5881 aggatcagca tcaccggatc acccttgcat atacccgcag acctgaagaa tccggcagcc
     5941 ttatcactgt agtatttcat ctgcgcgaaa gtgaaaacgg cctcgttgcc cttgtcgtca
     6001 caccatacca tggccgtctt ttctggttcg acccgggcta tttcatcaac aacatcccag
     6061 gcaaaattga aattctccgg tgtatcaatc tcaaagccgg aacaaaattc cccatacgac
     6121 ccaaactcag tttgctttaa gtatttattt ataatcatca tccagatccg aattaatcct
     6181 ataaaaccat tgccaggaat cttacctgct cattattaag ggccttcatt gcatgattgt
     6241 aaccggaatc aaaataaaca gcatctccgg cgttcaatac aatttcatgc cctcctatta
     6301 ccgtcagcat tgatccctcc aggacataat taaactcctg gccgggatgt gaactgaact
     6361 caggcaatga tccttccggc ttcggatcaa tcgtaaccat aaatacctca gcctttttgt
     6421 tgataaaatg tccggcaaga ctctcatacc ggtactgctg cctgcgctca agcttcagcc
     6481 cctttccttt cctgacaatg ctgaatatct gcagcctcgg ggcatccccc ccgagcagga
     6541 ccgacatatc aacattgaac atctcggata ttctgaatat tatgcccaca ggaatatccg
     6601 cttccccgct ttcatatttc aaataaagat ctgttgaaag acccagcttt aatgcaagag
     6661 tttctggcga aacctccgag atctcacgga tctcctttat ccttgtggca acctgcctta
     6721 tctgatctgt catgattccg ggtttttggg atctcaatat tacataaatt atttaattgt
     6781 gacctgaacg gcgtgctggt aaatatcaca ggggaagatc ggccatccgg cccgggcagt
     6841 cgttgtcaat acccgatgac aatcattgcc tgccggtata tcattgaccg ggaacagtcc
     6901 cggtatctga ataatcaatg ccgccttcct taccaattta ttctggcaat atttgatttt
     6961 tttaacccca tcggatgaaa tcccgcccgg ggagctgaat ggaaccgttt ttcagatttt
     7021 ttcaactttc cgtttttcag gccgataatt ccttttgtga attacctgtt aatagaaatt
     7081 cagacatttt gaattccatg tcttattttt ttcgcacttt cattttattt tcaaaaattc
     7141 agtttgtaat caaggtagtt gtaatatacg atctgaatcc ctcctgggat cacacagaaa
     7201 tcagggaagt ccggaactcc gccaggattc tttttgagtc acttcagctt gaagggatta
     7261 aagcctatat cgaggagctg aataacccgc acctggaaag gatccttgaa aaatacaatc
     7321 ccgataacac cataattttt aatctgtgtg aaagcctgcc cggattgcaa tacagtgaaa
     7381 aaaagattgt tgaaataatt gagaaccacg gattcaccta caccgggaat acgccggaag
     7441 tgattgggct gagctacgac aagcaaaagg taaaggatgt tctatcttcc atgggcatca
     7501 tggtgccccg cggtgcaata cttgacccgg aagaggctga aggatggaca ctttttccgg
     7561 caattgtaaa accctcccgc gagcactgca gccttactat cactgaaaaa tcagtggtct
     7621 atgacacttc ctccctcagg gaacagatcc tgctcgtaaa caatgaattg aaacaacagg
     7681 ccatggttga ggatttcatc gatggccgcg aattccatgt gtcggtttgg aacaatgagg
     7741 aaccggaaat gctgccccct gcagaaatgg atttttcagc tttccgtgaa gccaaagaaa
     7801 ggctgtgtac ctacgattcg aaatttatac cgggctcgga acattatgag aaaatccaga
     7861 cacttgttcc cgctcccctc gacggaaaac aattcaggaa acttgaaaaa acagcacttg
     7921 cagcttggaa aggattcgga tgccgcgatt atgccaggtt cgacttccgc ctgcgcaatg
     7981 atgaatttta cctcctcgac atcaatccga ataatgatat cagcgttgat acgagttttt
     8041 caatggcagc tgaaatacag aattactcct acagccagat ggtaaaacgg attatgctga
     8101 tggctgctga acgacatcct gaacttgcag aggaattcca gcttaataat ctgccggccc
     8161 gcggccattc aagaaaaagt atcgttgcat aaaagctgcc cgtatttcag tcttcccgtt
     8221 tttttgcttt tacctgcctc cggctgattg cttccgctgg tatcccagcc cctgaaaata
     8281 ccgtaacact ggcggcagat ttccccctac tgtctgccgc tgaccggaaa cattgatcgc
     8341 cccggtgaaa cgatactgcc tttgcggcct gtcatgatca cttttataag aaatttctgc
     8401 agcatttcac gggcatgctg cgtatatgtt atgtgattaa atcataaatc attaccaata
     8461 aaagacaatg acatgaaaac caatgtgata caagttctgg caatatttgc ggcacttatt
     8521 tttgccggct catgctcaaa aagtgacagt cctgaaccag atctgacacc agcgaatatt
     8581 aagctgactc ctgtagcact gcaggctatt cagagcagca atgaatttgg catcggtctt
     8641 ttcaccaggg ttgcagaaga agaaaatatg aacctgatgc tttcgcccct cagtgcaagc
     8701 acagtcctta caatgctgct caacggctgc ggtgaaaata catacaccca gcttaaggaa
     8761 atgctgaatt atccttccgg aatgactatc ggtgatatca atgaagccta caagagtctt
     8821 gtcggtcaac tcctgaaagc cgatccggat gtaaaacttt cactggcgaa tgctattttc
     8881 tacaggaacg gtttcactgt aaaacccccg tttcttgctg taatgggcaa tgactacagt
     8941 gccacggttg gcggactcga ttttgattcc ccgtcagccc tggcaacaat caataaatgg
     9001 gcaaatgaca ataccaacgg aaaaataccc aaagtgctcg acgaaatcga tcccgatgca
     9061 gtgatgttcc tcatgaacgc cctgtatttt aatggcgact ggagctatca gttcgataaa
     9121 tcacttacac aggaccgtcc tttctatccc gacggcagca gtacaataaa tgtaagtacc
     9181 atgaatagcg aagtgggtgc taagattgtt gcaggcaccg gcttcagggc cattgaaatg
     9241 ccctatggcc gtacaaattt cacaatggtg gtgattgtgc cggataaaac cctcgccgga
     9301 ttttatactt catttacccc actggcgtgg tcgcaactta ctggttcact ggacgccaag
     9361 cctgaattcg acaaaataaa ggtttatatg ccaaaattca aattctcata cgaaaaatat
     9421 cttaacagcc agttgcaaag catgggaatg gtcgatgcat tcatccctta ccaggcgaac
     9481 ctttcaggta tttccgacgc gtccatcttt gtcagcttcg tgaaacagaa cactttcgtt
     9541 gaggttgatg aggaaggaac cgaggctgct gctgttacaa caatcggagt aaatataacg
     9601 agtatgcctc ctgaaccgct ccagtttgta attgacaagt cattcatttt cgcgatacgg
     9661 gaacggacaa ccaacaccct gctctttatc ggacaggttg taaatccgga gaactaggac
     9721 acatgatttt caagtacgcc ataaagcctg caaaagtgtt ggatccgggt aataaagaca
     9781 taaggaaggt actgcaaggg tcttcccccc ggacaggcag gctgcagatt tcttaacaga
     9841 cagataacag ataacaggaa gagcccggat ggagaggaag cgaaaggccg aaggtcaggc
     9901 acccaacacg cactgaagcg atccctgatt actgaacacc gaatactgaa taccgattac
     9961 tgaatacata aaaaatcata aacacccgca accaacatga agacatttat cagaacatca
    10021 gtcacccttc tcatccttgc cgcatccctt cctgcctgca tggacaagac agtggaacgt
    10081 atcacctacg aggcaaatgt accgatatac atgccgttca ataaattccg ggccagcttc
    10141 agcaaaagtg agccggttga aataacagcc ccgggaaaat tctatttcaa ggataattat
    10201 ctttttgtta acgaggtcgg taaaggaatc catgtaatcg acaattcaaa cccggccaac
    10261 ccaataaaga tcgccttcta cgaaataatg ggcaacgttg acatggccat acggggaaac
    10321 atcctttttg ccgacagcta catcgacctg gttgcaatag atatctccga cattgaaaac
    10381 ccggtagaga ccggaagaat taaaaacatc tttcccgagg ttgttccgga aggcgatatc
    10441 tggtatcctt atgccatggt tgacaagtcg aaaggtgtta ttgtaggctg ggaagtaaaa
    10501 acagtcactg aggaacacga gcccggcagt aaatgggatg gctggatata ccgtaaggaa
    10561 atggtgatga tgggagctgc cgacagcagg gcaaactggt cggccggtgc aggaactgca
    10621 ggttcaatgg caagattcat gctgaatggc aggtatctgc atcttattgc ccatccgtgg
    10681 atgctgaaga ccgtcgatgt tgaagattca cgcaagatga atattgccga cagcgtaaat
    10741 gtttcccgca atatggaaac ccttttcctc cttgggcaga atatgtttgt cggaacaacc
    10801 acgggcatgc tgatatatga tgtatcagat cctgttaaac ccgtcctggt gtcacagtac
    10861 gaccatatca ccgcatgcga tccggtagtg gcggacggcg actatgcata tgtgaccctc
    10921 cgtacaggca ccagatgtgc caacgggcag aacctgctcg aagtgattga catatcatca
    10981 atcactagcc cctacctggt taaatcctat cccatgtaca atccgcacgg actggggatc
    11041 gacggcaatc tcctcttcat ttgcgacggg gcagccggcc tgaagatctt cgataagtcc
    11101 gatcccctcg atattattaa taaaaaaatt gcccattatc ccgatttcct gacatttgac
    11161 gtgataccaa tgaaaggtat actcatgctt gtgggagagg acggtatata ccagtataat
    11221 tattctgatc ccacaaacat tgtacagata agtcacatcc ctataacagg tgataaaaaa
    11281 taaaatatgt acggaacaag aatcaccgca gcaattgtgc tgttttttct gaccttcacg
    11341 ctaaatgcac aaaaccggtc aaaacagaca attgtcctta ataacggctc gcgcttgacg
    11401 ggtaccgttc tggctgattc ttccggttac cttaaactca gaattacagc cccgcaggtt
    11461 ctgacacttg aaaaatccgg tatcgcactg atcaaacctg acctgcgcac tgaaaaacct
    11521 gttaccgaca ggcatggtta ttcgataaga ctatctgcat cagtactggc agggcgcaac
    11581 cttgacggga acatccgcga tttaagtatt cacctgtcaa acggatatca gttcaggaac
    11641 ggaatatgtg caggtttcgg cagcggtatt gaaaaatttg atgaagtttc cgttctgcct
    11701 gtatacgccg atctcagata ccatcttctc aaatcacgcc tttcgccatt tttttgggtt
    11761 aagggcggca tttcattcac ctatgacgat cttggcgaca ggcaaaatta ttactatggc
    11821 tattattccg aatcaaaggg aggattcatg ttcaacactg gagcaggtat cgaactagcc
    11881 tcgtggcgga ggaatgccgt gaacatgggt gtgggttacc ggtatcagaa gatcactttc
    11941 aggcaagtga acaggtggaa tgaggaagtt ataaatgagc tagtttccag ttttaaccgc
    12001 attgagcttc aattcggtct tgtattcagg taaagaagaa ttatgcgcag ttaagtcata
    12061 aaaccatcca aattaagtta ttttgataca gcttaatccg gatggcagga atgagaaaaa
    12121 gtgataagga tgaacataca ctgtctgacc tggaacttat caggcgctgt tcggaaggag
    12181 atccgcgtgc acaggaactt ctttacaaac gctatttttc ttatgccatg tcagtctgca
    12241 tcaggtatac aaggaacagg tatgaggcca tggagatcgt taacgacagt ttcatgaaag
    12301 tgctggacaa cataagggag catgacggtt cgggatcctt caggtcatgg tacggcagga
    12361 tcctggtcaa ttcggccatc gataattaca ggaggaacgc aagacataat tcccatctgc
    12421 agattagcga gatagaatca accgaagact gcgaaccgga gatcagcgcg gaactgtcag
    12481 caaatgagat cctttccctg ttcgatctgc ttcccgatca ttaccgggta actttcaacc
    12541 tctatgagat cgaaggttat tcccatgaag aaatagggca aatgcttgat atcagctctt
    12601 caacctcaag gtcaaatctt tccagggcaa agaaaatgct cagggaactg tataaaagaa
    12661 acttcaatcc cggaaacagc agcaatgaac aggtttgacg acatattcag ggataatgtt
    12721 gaaaaggctt tcagcagcta caatgccgac catcttgcag aagagggatg gaattcgttc
    12781 gttgccggga aagcgaagcg gcgcaggcgg acaatatggt tcccgctgtg ggcgcgtgca
    12841 gcttcagtga tgctggtaat tggtcttggc gtttttattg catataaggc atccatacgg
    12901 cattctgccc cggagattct ctccggaccg ggatcatcgt caaatcttcc cgcggagtca
    12961 cccgcagaag ataaaacccc ggaaccgtct tctgcatcag ctgccagacc gtcgatttca
    13021 ggcacaacgc ccgtacagga taaaaccgaa aaaagccccg cctggcaaca gaattcttat
    13081 gaagaaaagt actcaccgtc agagtttatc ctcacaggcg ctgaacccct cccgcttccg
    13141 gaaatctcgc tcgacaggtt tattaatcgt tatggatccc cacctcctca tgttctggct
    13201 gagaacgaat tgcctgaaga taaaatacct gaagaccctg tttcagccgc cgtgatgcct
    13261 gaaggctttg tcatggataa aaactcatcc ggaggagaaa agcgtcacgg gacgggaatc
    13321 ctcatggcag gagtatcagg attgctggca cagagtgccg gaacagcgtc tccgtcatca
    13381 ggcctgtcgg ttggctttta cctcgatcag aagctctcag ggaaaatctc ggtccgtccc
    13441 ggactcgcca tagccatgca gtcgttcgga acaggggacg tcagcagcca ggacagattc
    13501 aattatacca tgtcgctgaa cgacggaaca aagggcatac ctcattcatc cagcggacat
    13561 ctgagcatgc tggcaatgga agtgccgctg aatattgttt tcaggatctc gggaaaggag
    13621 cgctcggggt tctacctgtc gaccggagca tccaccatga tctatctcag ccagcagttc
    13681 agggccggtt acgttaacaa atacacaaaa tcggagatca acaccatgac aggtgaatcc
    13741 ttttccgaaa cccgttattc gaccgttgag gtcgaaaata attacagcgc attcagccat
    13801 acagactttt tcggacttgc caacctgtcg gcaggatact ccttcgccta cggcaaaaca
    13861 ggcactatgc tcgtggagcc tttcctgcaa ctgcccgtaa gcgatcttac cagccttaac
    13921 ctcagagtca ggtacggagg tatttcaatg aaactgaagt tcggcagaca ggaacgggat
    13981 aattaaagaa ataattgctt ccggatttac cagggtcatc ccttccgcca ctgaaccccg
    14041 tggcctgaaa aacaccaccg ctatttatga aatgtaaaac taacgggaat atttctgccc
    14101 ctacttaatg ggcttcattt atacattatt ttccgaataa ttaatacctt tcgtgaaatg
    14161 aaactgcctg gcctaaccaa aatatttaac agaatgaaaa agctgttccg tgcactggtc
    14221 attatctttg cattgcttgt actgattgtc ataattaaaa ctgcgacatt cagttcgatg
    14281 cagctcaaac ctgaagcaat agagcttcct gctttccctg acgcaagcgt tgaacatctg
    14341 tcggaagcaa ttacatatcc cacaatctcg gaagcaattg attcccctgt tgacacagcc
    14401 gcttttaagg gattccacaa atttctcatg gaagcctatc cgctgataca ccgggatctt
    14461 aaaaaagaag tcttttcgtc attcagcctg ctctatacct gggaaggcag tgattcacgc
    14521 ctgaaaccgg tcgttcttac agcccacctt gatgttgtac caacgggcga attgagttca
    14581 tggtcggagc ctccgttttc cggaaaaaat gacggcacat tcatatgggg aagaggaacc
    14641 ctcgatgaca agtcctcaat gatatcaata atggaggctg ttgaaaagct tttgtccgaa
    14701 aactacaaac ccgaaagaac catttacctg gcatttggtc atgacgaaga gatcggcgga
    14761 ctgaaaggcg caggagtgat ggctggtgaa ctggtaaaaa gaaaccttga tgctgaattt
    14821 gttctcgatg agggttatgc agtaacaatc ggaatggttc caatgataaa aaaaccggtt
    14881 gcacttatcg gcacctcgga aaaaggatat ttgtcggtca gcctctctgt tgagatggaa
    14941 ggtggccatt cagcctttcc ggcaagggaa tcggcaatta ccctgatcac aggagctttg
    15001 aacaggatcg tcagcaacca gatgaaatcc gggatatcag aaccagtaaa tgactttatc
    15061 aggtatatcg gcccggagat gccctggtat gcaagggcaa tatttgccaa caaatggctt
    15121 ttcaaagggc tgatccttaa aatatatgag ggaggaaata actccaacgc ccttgtaagg
    15181 acaaccaccg ctccgacaat tattcatgcc ggaattaaag acaacgtgat acccacccgg
    15241 gcggaagcag ttgtgaattt caggatactg ccaggtgaaa cctcaggtga tgtcataaat
    15301 caccttgaaa gggtcattgc tgatgaccgg atcagcatcc ggccgcttga agacctgaaa
    15361 gagcctgcac ccgtttcccc gattgatgca cccggatttg atattattca caagaccata
    15421 agacaggttt atcccgaggc acttgttgca ccaacaatga tgctggcagc ctcagacagc
    15481 aggaagtatc tgggaatcag caggaatatc tacaatttcg caccgatcat tgttaccgat
    15541 gaggatcttg cgagaacaca cgggctaaat gaaaggactc aaatcgggca tttcatccgc
    15601 ggcgtcggtt tttactacca gctgatcagg aactgcaaca atcaatcagc cggctgatt
//
LOCUS       DAOOYG010000058        15529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3764_length_15529_cov_7.418832, whole genome shotgun sequence.
ACCESSION   DAOOYG010000058 DAOOYG010000000
VERSION     DAOOYG010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15529
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_15529_cov_7.418832"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PK288_06585"
     CDS             complement(<1..485)
                     /locus_tag="PK288_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HPM18624.1"
                     /translation="MKNVYLPAIILSVLMFFSCSQEKPFKKIVDDSLDFSLKQYGLMT
                     DVMKSMPGQLPRTIDSEGKLITESSSWWTSGFYPGSLWYLYEYSNDPGLKGNAVEMSM
                     RVEKEKYTTDNHDVGFILYCSFGNGLRLTGEESYNEILMTGARSLCTRFRPVTGCIQS
                     W"
     gene            1101..2036
                     /locus_tag="PK288_06590"
     CDS             1101..2036
                     /locus_tag="PK288_06590"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18625.1"
                     /translation="MMKKTLFLFIGFLVSFHYASAQNIGSSPEYIKALTAEWQGERFP
                     DGRPKVSDRLLERLKNISMEEAWGVLRSKGYMNQFESGWMILNPDEPMTGRVVTAQYM
                     PLRPDYDKYIREQGKRENRAQQGGSNSWPIDVLVEGDVYVADSYDKIVDGTLIGDNLG
                     NSIYAKSKRGVIFWGSVRDAEGLDDIEGFNAWIRGADPSYITQMMLTSINAPIRVGRA
                     TVMPGDVVLAKKYGVIFIPAHLVEELVITSEVTALRDKFGHQRLKEGKYKPGEIDSSW
                     SNEIKKDFLNWLRNYPGQLPMSQQELDNYFKERHW"
     gene            2084..3556
                     /locus_tag="PK288_06595"
     CDS             2084..3556
                     /locus_tag="PK288_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HPM18626.1"
                     /translation="MKGTLKHILESERKRENEELQKSYASGSTGESSRRGFLKKAALG
                     GIALGGFMYSSIEDTIAQTTSGVNRASAPSELKITDMRYATVMGKHIIRIYTNQGIYG
                     LGEVRDGADVRYALFLKSRILGLNPCNVEMIFKIIRQFGYHGRQGGGVCAVEMALWDL
                     TGKAYNVPAWQLLGGRYRDKVRLYADTPEVDDPVKFAEVMKKRVGEQGFTWLKMDLGI
                     HVVNKIPGALVNAEFWNGAAGQYDLREYMGYGNTLHPFTQIQITDRGLEGLQEYVAKV
                     RDGVGYEIPLCADHFGHFDLNNSIRFGKAMEKFRLAWLEDMVPWFSTEQWKKVTDALE
                     TPTCTGEDIYCLKDGFKPLIDAQAVDIIHPDLGTSGGLLETKKIGDYAEEHGIAMAMH
                     MAGSPVCFMANVHCAAATQNFLALGHHSVDVPYYNNLVKMTGSKPMIEKGFANVPLDA
                     PGLGIELNEEECKKHMPKGAGWFEPTPEWDQKRSHDRLWS"
     gene            3932..5695
                     /locus_tag="PK288_06600"
     CDS             3932..5695
                     /locus_tag="PK288_06600"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPM18627.1"
                     /translation="MSQNKRFDISGLQGKPSSIRDRMRTWRIPPRLLFFLMGIVSTAW
                     FLLRVIPKPSRAAYPCMKVAAPLMSGFVLYLISMGGAVVAFRKAGRNLYRARYLAAFL
                     FLVAAAAGIVVSLLHGSFNLSAATTQVSGPADGPNQPMGTARGANPGRVVWVWNPDAT
                     NENCMNNFDRQDWFFKPENTNGRIVGDMVRSSLMELTGKKNIRESWDVLFKTHNLRKY
                     GLDSGYRKGQKIFIKINQGTSRLGLTPEEKDNGFYYPSTLKPGDEKRKPLYGATETGP
                     YVVLELLRELVYEVGVDQEDIGVGDPMTDICGHNYEVWHGEFPGIVYTGKYSDKHGRT
                     LIKPTEKDLLFYSDKIYTDKLYDVLENADYLINSACLKPHMSAGITLTAKNHFGSQAR
                     ENAIHLHYSLMVPIGSRPLNTGYRKYRVLVDLMGSKYLGQNTVLYLVEGLFGGGSSET
                     RTPVKYLSPPFCNDWCSSVFVSQDQVALESVCFDILRNEWNGKNKHDPANNASEDRPN
                     LHGVDDYLHQAADRANWPVNIIYDPDNSGKPLPSLGTHEHWNNPKDRQYSRNLGRNSG
                     IELVTIPDSLVKKSVTSGTIN"
     gene            5731..6861
                     /locus_tag="PK288_06605"
     CDS             5731..6861
                     /locus_tag="PK288_06605"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF015727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HPM18628.1"
                     /translation="MKNNRRDFIKKGGALAALSIAGLNSCQTNTSRKQDKEQSYLKDR
                     SDVSVREPSIKIAYQAPAEPGENDISFIRQMGVDYVVLWTDAKKAGYEYYNSRRELYE
                     SHGIKVFGFGNWDVHNQDKITLNLPGRDEKIEEYLAHLRNLSKAGIYYTTYAHMANGI
                     WSTANEKTRGGAVARAFNLDSADAGYWHGVRYEAPLSHGRKYSKDELWANYEYFIKKA
                     AATAEEYKVRIGIHPDDPPVEELGGIPRCIFGNFDGYKRALEIADSPYVGMCLCVGCW
                     LEGGTMMGRDVIETIKYFGERKKIFKVHFRNVNQPMPHFTETFLDDGYADMYQILRAL
                     KEVKFDGVIIADHIPAMVHPHSGTAFSIGYMKALVERVTAEG"
     gene            7148..8452
                     /locus_tag="PK288_06610"
     CDS             7148..8452
                     /locus_tag="PK288_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HPM18629.1"
                     /translation="MKKLNPHKAALGFALLMLVIILITALTGNGAIAGPLLIIMFITL
                     SYAVKGFPAVKGLSFTMWMFTAVCAAMFYPGLFVEIGGFQTKALIVPLLQIIMFGMGS
                     QMSLNDFTGVIKMPKGVVIGVLGHYIIMPLMALLITKVFHFPAEIAAGIILVACVPSG
                     LASNVMSLLANANLALAVTIGALTTIIAPFVTPFLMKIIGGQFVAIDIWKMMLDIIDM
                     MIFPIIAGFIFNLIWGGKGLTRKAITELASYLAIIFLGNLIFLKVKDSGFTGYLLENA
                     KIIGIFFILPIVGALAFRYFLKDNKQAMKKILSMFSMVGIAVIVTVITAAGRDSLLQV
                     GALLLIMTILHNLGGYALGYGLAWSFRMPEQDRRTIAFEVGMPNAGLASGLAITIGNI
                     ATIGLAPAIYGPLMNVTGSTLASWWRTKPTHKEEDTEKKINN"
     gene            8477..9514
                     /locus_tag="PK288_06615"
     CDS             8477..9514
                     /locus_tag="PK288_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPM18630.1"
                     /translation="MNHRIVTFGEIMLRLATPGFERFSQIKQFNASFGGGEANVAVSL
                     ANFGLKTDYVTRLPKNDIADSCIMELRRLNVGTDKIIFGGDRIGIYFLENGAVARPSK
                     VIYDRANSAIAEIKPGMVDWDSVFEGATWFHWTGITPAISEGAALACKEAIEVANKKG
                     ITVSTDLNFRKNLWKWGKKAPEVMPDLVSGCDIILGNEEDAEKVFGIHPEGVDVTKGH
                     VEAAAYESVCRQLMQKFPRAKKVIITLRGSINANHNTWSGVLWDGKKLYEAPVYDITH
                     IVDRVGGGDSFMGGLIYGLITFPGDDQRALNFAVAASCLKHTIHGDFNMVNVKEVETL
                     MGGDASGRVSR"
     gene            9573..10262
                     /locus_tag="PK288_06620"
     CDS             9573..10262
                     /locus_tag="PK288_06620"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPM18631.1"
                     /translation="MARFTRIQVALRMAEEGMIPVFFNSNAEVCKKVIEACYKGGIRL
                     FEFTNRGDYAHVVFEQLNKFAAAEFPDMIIGTGSVIDAPTAILYVQLGSNFIVSPILN
                     EEMAGVLNRRKILWSPGCGSASEISRAEALGAEIVKIFPGSQVGGPKFVAAMKGPMPW
                     TQIMPTGGVEPTEANLTEWFKAGVVCVGMGSQLIKSELVKAGDFKKIEEDVRNAVALV
                     KRLRQQYPVKI"
     gene            10309..10764
                     /locus_tag="PK288_06625"
     CDS             10309..10764
                     /locus_tag="PK288_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HPM18632.1"
                     /translation="MIADIIKNRQLYSAISPGIARGLEYIAGTDFSAMEPGRYEIDGA
                     NLYALVQVYDSIPKEQGKWECHRNYIDIQYIADGVEQIGVNHTGKMHVITPYNPEKDI
                     EILGGDGDYITLSKGAYGIFFPEDAHQPKIAPANQPGPVRKVVVKVRIG"
     gene            11016..11915
                     /gene="rbsK"
                     /locus_tag="PK288_06630"
     CDS             11016..11915
                     /gene="rbsK"
                     /locus_tag="PK288_06630"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPM18633.1"
                     /translation="MNRIIVIGSSNTDMVIKAKKLPAPGETVLGGTFLMNPGGKGANQ
                     AVAAARLEGKVTFVTKTGNDLFGEQAIHLFKFEGIDTRFIIRDPKNPSGIALITVDEN
                     GENCIVVAQGSNATLAAYDIEDAAFEADSHDVFLMQLEIPVSTVEFVAQKVFNKGIRV
                     ILNPAPARLLPNELLECLYLITPNEIEAELLTGIKVTDAASARTAAGKLLHKGVRNVV
                     ITMGAAGAFLMNQDFAGMVPVVPVKAVDTTAAGDVFNGALAVAIAEGAELKDAVDFAN
                     RAAAISVTRMGAQSSAPYRKELQ"
     gene            11975..12976
                     /locus_tag="PK288_06635"
     CDS             11975..12976
                     /locus_tag="PK288_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HPM18634.1"
                     /translation="MMTNKILFLIFLVLTLGGCLNPASRNETGIPKIIFDTDLGPDYD
                     DVGALAFLHAMADSGKAEILATISSNKHELVAPSIEIINTYFKRPELEIGAPKSEGAN
                     MGSTQHWADSLVANYPHKIRSTDEVQDAVSLYRKILNSQPDGSVTIVTVGFLTNLANL
                     LRSEADAVSPLSGRDLVSKKVKLLVSMAGKFPEGREFNVYIDSLSSKYVFDNWPGKII
                     FTGWEIGVEIRTGLKVMQLPVSGSPVKDVFKISIPMSEEDRMGRMSWDETAVLIGVYG
                     TDGFFDTVRGKIIVNTDGSNGWTDDPDGNQFYVVQKMPVQDMAGFIEERIMHIPLGK"
     gene            13149..14765
                     /locus_tag="PK288_06640"
     CDS             13149..14765
                     /locus_tag="PK288_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPM18635.1"
                     /translation="MKKIFGFLSVILVLGMFSCKESGTGFRKIDPAVLKDKIAGGWAG
                     KMIGVTYGAPTEFKAGGKTYEDPINWKPEDIKGSIWQDDIYVQLTFLMTMDKFGMDAS
                     QKQFQEMLAKAGYPLWHANMQARKNYLDSIFAPLSGNPEYNIHADDIDFQIEADYIGF
                     MCPGMPRTASGIADKIGHIMNYGDGVYGGIFVAALYAEAYFEPDIRKIVKQALRSLPE
                     ESDYHKIVEDVIKLHGHYPSDWRAAWQELENKWGDVDICGAGTDFNIDAKLNGAYIVM
                     GLLYGDGDPYKTLEISTRCGQDSDCNPSNALAVLGVINGFSGLPQEMQDGVKAIGDSL
                     FIFTTYSFNSAVQSTYNYALDLISKEGGRVTGNRILIRPQEPVRPRLEVAFPNILFDK
                     KISVFEKGGFRFTGAWKPLEVNYGAGSVPVRQSMYSDRPGDELVLEFSGTGISLTGNL
                     YRDGGKADVYIDGKLHRTIDTYYYFGNQQQPEESMWHIMGLTPGEHKVRLVVKGEKRP
                     ESSGTRIYVSSANIFRTGSKKNENFRFSFE"
     gene            complement(15076..15468)
                     /locus_tag="PK288_06645"
     CDS             complement(15076..15468)
                     /locus_tag="PK288_06645"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HPM18636.1"
                     /translation="MKRFPGNFMFQLTEKEKEKMVVICDHLQNLKYSPYLPYAFTEHG
                     SVMLANILNSDRAIEASIRIVEIYIKMRELVLSHKDILLKLEQLEKRVGKQDENIAMI
                     LQYLRKFIDVQERPRKTIGYKQSGTDKQ"
BASE COUNT         4413 a         3463 c         3868 g         3785 t
ORIGIN      
        1 tcccatgact gtatacaccc tgtaacgggc ctgaaccgtg tacacagcga ccgggctcca
       61 gtcatgagaa tctcattgta actctcttcc ccggtgagtc tcaatccgtt accaaagctg
      121 caataaagaa tgaaacccac atcatggttg tcggttgtgt atttctcctt ttcaaccctc
      181 atgctcatct cgacggcatt ccctttcagg cccggatcat tggaatattc atacaggtac
      241 cacagactgc cgggatagaa tccgctggtc caccatgagg aactctctgt aataagtttt
      301 ccttcactgt caatagttcg gggcagctgg ccaggcatgg atttcatgac gtcagtcatc
      361 agcccgtact gtttcagtga aaaatcaagc gaatcatcaa cgattttttt gaaaggcttc
      421 tcctggctgc aggaaaaaaa catcaaaact gaaaggataa ttgccggcag gtaaacattc
      481 ttcattttaa aagtcagtta agttattgaa attatagttc aggtttattt ttctttattc
      541 tgaatattac aggtgcaggg catctgcgca aaatgatcct ggtaccgcaa tactgaaaaa
      601 tcccgtcccg taattcgtca ttcgtgtcct gcccgctcga accagtcagg ccccgaaaca
      661 acgaaccgct aatttaatca atggttaatt tccgccaaat taataatttt tcccgccggt
      721 gctaaaataa gtgattaaat cctgattatt ttaatatctg cggattatgg atgaaactga
      781 cgaggtccgg gcagctatca tgtgttcgta cccgctgcaa ttaaggactg aagcgatccg
      841 gtttcctgaa tgtcgaaaaa agaagggata ctaaaacaaa ataaatggct ttaattcaaa
      901 aaactgtcta tatcgatttg ccgaatcaga aaaattcttt taattgccgg cgttaatgtc
      961 agatttcagg cagttaaccc tgaatcccta ttgaaccaat tattatacaa cacaaaaaca
     1021 tgtataaata ccagttcggc attggagttt ctccccatgg ggtcaggcag gtttcggacc
     1081 attaatttaa aattataatt atgatgaaaa aaacgttgtt ccttttcatt gggtttctgg
     1141 tttccttcca ctatgcaagt gcccagaaca ttggctcatc accggaatac ataaaagccc
     1201 ttaccgcaga atggcagggg gaaaggtttc ctgacggacg gccgaaggtt tccgacaggc
     1261 tgctggaaag gctgaaaaat atttccatgg aagaagcatg gggggttctt cggtcaaagg
     1321 gatatatgaa ccagtttgaa tcaggctgga tgatcctgaa tcctgatgag cccatgacgg
     1381 gacgggtggt cactgcacag tatatgccgc tcaggcctga ttatgacaaa tatatccggg
     1441 agcagggcaa gcgggaaaac agggcccagc agggcggatc aaattcctgg ccgattgatg
     1501 tcctggttga gggggatgtc tatgtggcag acagttatga taagattgtt gacgggacgc
     1561 tgatcggcga caatctgggc aactcgatat atgccaaatc gaagcgggga gtaattttct
     1621 gggggtcggt gagggatgcc gaaggacttg atgatattga aggtttcaat gcatggatca
     1681 ggggagctga tccttcgtac atcacccaga tgatgctcac atcaatcaat gcaccgattc
     1741 gtgtgggaag agccactgta atgcccggcg atgtggtgct ggcaaaaaaa tacggcgtta
     1801 ttttcatccc tgctcatctg gtcgaggagc ttgtaattac ctcggaggtt accgctttga
     1861 gggacaaatt cgggcatcag aggctgaaag agggtaagta caaacccgga gaaatagaca
     1921 gcagctggtc aaatgagatc aaaaaagact tcctgaactg gctcaggaat tatccgggcc
     1981 agctcccgat gagtcagcag gaactggata attattttaa ggaacgtcac tggtaatgca
     2041 gaatcagaat cagaatcaca ttaaaaacta aatccaaacc atcatgaaag gcactttgaa
     2101 acacatcctt gaatcggaaa ggaaaaggga aaatgaagag ttacagaagt cgtatgcttc
     2161 cggcagcacc ggggaaagca gccgccgcgg attcctgaag aaggctgccc tgggaggaat
     2221 tgcacttgga ggattcatgt actcatcaat tgaggacact atagcccaga caacctctgg
     2281 tgttaacagg gcctccgcac cctccgaact taagataacc gacatgcgct atgccactgt
     2341 aatgggaaaa catattatca ggatctacac caaccagggt atttacgggc ttggcgaagt
     2401 aagagacgga gctgatgtca ggtatgcatt gttcctgaaa agcaggatac ttggtctgaa
     2461 tccctgcaat gttgaaatga tcttcaaaat aatcaggcag ttcggttatc atggccgcca
     2521 gggcggcgga gtgtgtgccg ttgagatggc tctgtgggat cttaccggaa aggcgtacaa
     2581 cgttcctgca tggcagcttc tcggtggcag ataccgcgac aaagtcaggt tatatgccga
     2641 tacacccgag gttgacgatc ctgtaaaatt tgccgaggtc atgaagaaaa gagtcggaga
     2701 acagggattt acatggctca aaatggatct cggcatccat gttgtgaaca aaataccggg
     2761 agccctggta aatgcggaat tctggaacgg agcagccgga cagtatgacc tgagggaata
     2821 catgggatac gggaacaccc ttcatccgtt cacccaaatc cagataaccg acaggggtct
     2881 ggaaggattg caggagtatg tggcaaaggt aagggatggt gtgggatatg agatccccct
     2941 ttgtgccgat catttcggac attttgacct taataactcg ataaggtttg gtaaagccat
     3001 ggaaaaattc aggctggcat ggcttgagga tatggtacca tggttcagca ccgaacaatg
     3061 gaagaaagtt actgatgccc tcgaaacccc cacttgcaca ggggaggaca tctactgcct
     3121 gaaggatgga ttcaaaccgc tcatcgatgc tcaggcagtc gatataatcc atcccgacct
     3181 tggaacatcg ggcgggctgc tcgaaacaaa aaagattggt gattacgccg aagaacacgg
     3241 aattgcaatg gcaatgcata tggcaggatc gccggtttgc ttcatggcaa atgttcattg
     3301 cgctgcagca acacagaatt tcctggctct tggacatcat agcgtggatg tgccatatta
     3361 caacaacctt gtgaagatga ccggttcaaa accaatgatc gaaaaaggtt ttgccaacgt
     3421 accgcttgat gccccggggc tcggcataga actcaacgag gaagaatgca aaaaacatat
     3481 gccaaagggg gccggctggt ttgaaccaac tcctgaatgg gaccagaaac gctcacacga
     3541 caggctctgg agctaatcgg gaaaaagact tcttataaga ttggctataa gactacatat
     3601 tatcctgtgg ctgttgagca aatatcttta accgcaggga acgcagcatg catccgccag
     3661 gcatcaggcg catggatatt ccaggcggaa attgatatgt ctgcggttcc tgtagttatt
     3721 ttatcctttc gttcagcaga gtatattcct gatttatcct gtaaataata actataatct
     3781 taacggtgtg gcagggaaaa gacaagaaaa atcgctattt tcacatcggc tgatccgttg
     3841 cctttggatt tatcaggcaa ttctccgtat actcaacatg tatgaacaac agtaatatac
     3901 agttcttcag attatcagca aacttaaggc tatgtcgcaa aataaacgat ttgatatttc
     3961 cgggcttcag gggaaaccct cgtcaatcag ggaccggatg agaacgtgga gaatacctcc
     4021 ccgtctcttg ttctttctga tgggaatagt ttcaaccgcc tggtttctgc tgcgtgtcat
     4081 tcccaaacct tccagggcag catatccctg catgaaggtt gcggcaccgc tgatgtcggg
     4141 tttcgtatta tacctcattt ccatgggcgg ggctgttgtg gcattcagaa aagcaggccg
     4201 gaatctttac agggcccgtt acctggctgc ttttcttttc ctcgttgcag ctgcagctgg
     4261 aatagttgta tcccttctcc acggatcatt taatttatca gcagctacaa cccaggtctc
     4321 cgggcctgcc gatggtccca atcagccaat gggtaccgca cgtggtgcaa acccgggcag
     4381 ggtagtctgg gtctggaatc ctgacgctac caacgaaaac tgcatgaaca atttcgatcg
     4441 tcaggactgg ttcttcaaac cggagaacac caacggcagg atagtaggtg atatggtcag
     4501 gagctccctc atggaactta ccggcaaaaa aaatatccgt gaatcatggg atgtcctttt
     4561 taagacccat aacctgagaa aatacggcct ggattccggt tacaggaaag gacagaaaat
     4621 atttatcaag atcaatcagg gtacttcacg actgggactt accccggagg agaaggataa
     4681 cggattttac tatccctcca ccctgaaacc aggcgatgag aaaagaaaac ccctgtacgg
     4741 cgcaacggaa accggacctt atgtcgtact cgagctgctc agggagcttg tctatgaagt
     4801 gggtgtagat caggaagata tcggggttgg tgatccaatg accgatattt gcggacacaa
     4861 ctatgaggtc tggcacggtg agtttcccgg tatagtctat actggaaagt attccgacaa
     4921 acatggaagg accctgatca aacccacaga aaaggatctt ctgttctatt ccgacaaaat
     4981 ctacaccgac aagctttatg acgttctgga aaatgccgat tacctgatta attcagcctg
     5041 tcttaaacct catatgtcag ccggcattac cctaaccgcc aaaaaccatt tcggttcgca
     5101 ggccagggag aacgcaattc acctccatta ctccctgatg gtgcccatcg gttcaaggcc
     5161 cctgaacacg ggctaccgca aataccgcgt tcttgtcgac ctgatgggaa gcaaatatct
     5221 cgggcaaaac acagtattat acctggttga aggcctgttc ggcggaggat ccagtgaaac
     5281 aagaaccccg gtaaagtatc tgagtcctcc tttctgcaac gactggtgca gctcggtatt
     5341 tgtatcacag gaccaggtgg ctcttgaatc tgtctgcttc gatattctga ggaacgaatg
     5401 gaacggaaag aacaagcacg atcccgctaa taatgcgtcg gaagacaggc cgaatctaca
     5461 cggagtggat gattatctcc atcaggccgc cgacagggca aactggcccg tgaatattat
     5521 ttacgatccc gacaacagcg ggaagccgct tccaagcctg ggtacccatg aacactggaa
     5581 caatcccaaa gacaggcaat actccaggaa tctggggaga aattccggga tagagcttgt
     5641 tacaattcct gattcactgg taaaaaaatc agtgacttcc ggcacgataa attaaagaat
     5701 gaaccataac cgtaacctga tcaacctgca atgaaaaaca accggagaga tttcatcaaa
     5761 aaggggggag cacttgctgc cctgtcaata gcaggcctta attcctgcca gacaaatacc
     5821 tcacgcaaac aggataagga acagtcatac ctgaaggacc ggtctgacgt atctgttcgt
     5881 gaaccctcca taaaaatagc ttaccaggca ccagccgagc cgggtgaaaa tgacatcagc
     5941 ttcatccggc agatgggcgt agattatgtg gttttatgga ccgatgcaaa aaaagcaggt
     6001 tatgaatact acaacagccg gcgtgaattg tatgaatcgc atggaataaa ggttttcggt
     6061 ttcggaaact gggatgttca taatcaggac aagataaccc ttaatcttcc cggaagggat
     6121 gaaaaaatag aagaatatct tgcacatttg cgcaatcttt caaaagccgg aatatattac
     6181 acaacctatg cacacatggc caacggtatc tggagcaccg ccaatgaaaa aacgcgtggc
     6241 ggtgccgttg caagggcctt caatctcgat tcggcggatg cgggatactg gcacggcgta
     6301 aggtacgagg ctccgctttc gcatggaagg aaatattcga aggacgaact ctgggccaac
     6361 tatgagtatt ttataaaaaa ggcggcagcc acagctgagg aatataaggt ccgcattggc
     6421 atccatcccg atgatccccc agttgaagag cttggaggta taccaagatg catattcggt
     6481 aatttcgacg gctataaaag ggccctggaa atcgccgaca gcccatacgt gggaatgtgc
     6541 ctttgcgtgg gctgctggct cgaaggcggt acgatgatgg gaagagatgt gattgaaaca
     6601 ataaaatact tcggtgagcg aaaaaaaatc ttcaaggtgc atttccggaa cgtaaatcag
     6661 cccatgccgc attttaccga gacattcctc gacgacggct acgccgacat gtatcagata
     6721 cttagggcac tgaaggaagt aaagtttgat ggcgttatta ttgccgacca tatcccggcg
     6781 atggtacatc cgcattcagg aacagctttc tcaataggat acatgaaagc cctggttgag
     6841 cgcgtaactg ccgaaggatg atttttttac ctgctataac caatctctag cgactttgtg
     6901 agggcttaaa gttcatttca ccatttgggt gatatttcaa tatatttctt caaacagagt
     6961 tttgtattaa aaaagtttaa aatagttccg ggagttaagg ttaaattagg ctaataagaa
     7021 agcaagaatt atatttgtct gaggtgaatt cccattaaca taactaggac aggctgacag
     7081 gccattgatc ggagactgaa aacagactaa ttattaatca acctatttat ttataacaga
     7141 ccagcttatg aaaaaactga accctcacaa ggcagctctt ggctttgcct tactcatgct
     7201 tgttattatt ctcataacgg cattaaccgg caatggtgca attgccggac ctctgctgat
     7261 aataatgttc ataacgctgt cctatgctgt gaagggattt cctgcagtaa agggcctgtc
     7321 attcaccatg tggatgttca ccgctgtttg tgccgcgatg ttctatcccg ggctgtttgt
     7381 ggagatcgga ggtttccaga ccaaagcatt aatagtgcct ttgctccaga taataatgtt
     7441 cgggatgggt tcgcagatga gcctgaatga ttttaccggg gttattaaaa tgcccaaggg
     7501 cgtggtaata ggtgtacttg gacactacat aatcatgcct ttgatggctc ttcttatcac
     7561 caaagttttc cattttccag ctgagatagc tgccggaata atactcgttg catgcgttcc
     7621 cagcggccta gcctccaatg taatgtcact cctggcaaat gccaaccttg cacttgctgt
     7681 aacaattggt gcactgacaa cgatcatcgc accttttgtt acgccctttc tcatgaaaat
     7741 aatcggagga cagtttgttg cgatagatat ctggaagatg atgctggata ttattgacat
     7801 gatgatcttt ccgataatcg ccgggttcat attcaacctg atctgggggg gaaagggcct
     7861 gacacggaaa gccataacag agcttgcctc ctacctggcc ataattttcc ttggaaacct
     7921 tatttttctt aaggttaagg acagcggatt cacaggttac ctgttggaaa atgcaaaaat
     7981 aattggaata ttttttatac tgcccattgt cggcgcgttg gcattccgat acttcctcaa
     8041 agacaataaa caggcaatga agaaaatact ttcaatgttt tccatggtcg ggattgcggt
     8101 tattgttacg gtaataacag ctgcgggacg ggacagcctg ctccaggttg gtgcattact
     8161 gctgataatg accattctgc ataatcttgg aggctatgcc ctcggatatg gtcttgcctg
     8221 gtcattccgg atgcctgagc aggatcgcag gacaattgcc ttcgaggtcg ggatgcccaa
     8281 tgcaggactg gcatccgggc tggctatcac aataggaaat atcgccacaa taggccttgc
     8341 tccggcaatt tacggcccgc tgatgaatgt gaccggatca accctcgcca gctggtggag
     8401 gacaaagcct actcacaagg aggaggacac agaaaagaaa atcaataatt aattaaaaaa
     8461 ccataaaatt cacactatga atcacagaat cgtaactttc ggagagatca tgctgaggct
     8521 cgcaactccc ggttttgagc gcttcagcca gataaagcag ttcaatgcct cattcggagg
     8581 aggtgaggcc aatgtggctg tttccctggc caacttcgga ttgaagaccg actatgttac
     8641 caggctcccg aagaatgaca tcgcggattc ctgcatcatg gaactcagaa ggctcaatgt
     8701 ggggactgac aaaatcattt tcggcggaga caggataggc atatatttcc tcgaaaacgg
     8761 tgcagtggca cgtccaagta aggtgatcta cgaccgtgcc aattccgcca ttgctgaaat
     8821 caagcccggt atggtagact gggattcggt atttgaaggt gcaacatggt ttcactggac
     8881 aggaataacc cctgcaatat ctgaaggagc ggcacttgca tgcaaggagg ccattgaggt
     8941 tgccaacaaa aaaggaataa ccgtatcgac agatcttaat ttcaggaaaa atctctggaa
     9001 atggggcaaa aaggcgccgg aagtaatgcc cgacctggtg tcaggttgcg acattatcct
     9061 tggcaatgag gaagatgcgg agaaagtgtt tggtatacac cctgagggag tggatgttac
     9121 aaaggggcac gtggaggcag cggcctatga atcggtgtgc cgccagctga tgcagaaatt
     9181 cccgcgggca aagaaagtca taatcacact ccgcggatcg atcaacgcca accataacac
     9241 ctggtcgggt gtgctttggg atggtaagaa actttacgaa gcacccgtat acgacataac
     9301 ccatattgtt gacagggtcg gaggcggtga cagcttcatg ggcggactta tttacggtct
     9361 catcactttc cccggcgatg accagcgcgc cctgaatttt gcagttgcgg cttcttgcct
     9421 caaacatacc atccatggcg acttcaatat ggtaaacgtc aaagaggtcg aaactctcat
     9481 gggcggcgat gcctcaggaa gagtgtcaag gtaagccctt gaaaatgtct caacagaaat
     9541 aatttacgga tataaatatc aaataatata caatggctag atttacaaga attcaggttg
     9601 ccctgaggat ggcagaagaa ggaatgatcc cggtcttctt caacagtaat gcagaagtgt
     9661 gcaagaaagt aattgaagca tgctataaag gcgggataag actattcgag tttaccaaca
     9721 ggggcgatta tgcacatgtg gtctttgaac agctgaacaa gtttgctgca gccgagtttc
     9781 ccgacatgat cattggcacc ggctcggtca ttgatgcccc cacagctatt ctgtatgtac
     9841 agttggggtc caacttcatt gtgtcgccga tactcaatga ggaaatggcc ggggttctca
     9901 acaggaggaa aatcctctgg tcgccgggct gcggatcagc ttctgaaata tcaagggcag
     9961 aggctctcgg ggccgaaatc gtaaaaatat tcccgggcag ccaggtcggt ggtccgaagt
    10021 tcgtggcagc aatgaagggc ccgatgccat ggacccagat aatgccaacc ggcggcgtgg
    10081 aacccactga ggccaacctg accgaatggt tcaaggccgg ggttgtctgc gtaggcatgg
    10141 gatcacaact gatcaaatcc gaacttgtca aagccggaga cttcaaaaaa atcgaagaag
    10201 atgtcaggaa tgcagttgcc ctggtgaaga gactgaggca gcagtacccg gtaaagatct
    10261 gaaaacagct gaaattacag taaaatccaa cttaaaaaaa cttctgaaat gatagcagac
    10321 attataaaaa acagacaatt gtactcggca atatcgccgg ggatcgccag gggacttgag
    10381 tatattgccg ggaccgactt ctcggcaatg gaaccaggca ggtatgaaat tgacggggca
    10441 aacctttacg cactggtaca ggtgtacgac tcgataccca aagaacaggg aaaatgggaa
    10501 tgtcacagga attatattga tattcagtat atcgccgacg gtgttgaaca gattggcgtc
    10561 aatcacacag gaaagatgca tgtcatcact ccctacaacc cagaaaagga tattgagatc
    10621 ctgggtggcg acggggacta tattaccctc tcaaagggag catacgggat atttttccct
    10681 gaagatgcac atcagcccaa aatagctccg gccaaccaac ccggtccagt caggaaagtg
    10741 gttgtaaaag tcaggatcgg ctgaggaatc gtcacggacg gacggggaga aacgcaggac
    10801 aacacaggag agtgaggaat attttatgtt agtactcctt accgtcctgt gtttttttgt
    10861 cccgtgccat ccggatcagt ttacatgctt ctccggtatc atttaattac agccatgcgt
    10921 tatttccgct gccggccttt tttgactgtt acaatttatg tatttattta aaggaaaaat
    10981 ttatcttagg gatctgtatt tttactcgca gccaaatgaa cagaataatc gtcatcggaa
    11041 gctccaacac cgatatggtg atcaaagcaa aaaaactgcc ggctcccggt gaaaccgtac
    11101 tcggcggcac ttttctcatg aacccgggag gcaagggagc aaaccaggct gttgcggctg
    11161 caaggctcga aggcaaggtg acatttgtca caaagaccgg gaatgacctg ttcggggaac
    11221 aggcaatcca tttgtttaaa tttgaaggta tcgacacaag attcataatc agggatccca
    11281 aaaatccatc cggaattgct ctgattacgg tagatgagaa tggtgaaaac tgcattgtag
    11341 ttgcccaggg ttcaaatgcc acacttgcag cctacgacat tgaggatgcc gcctttgagg
    11401 ctgattcgca tgatgttttt ctcatgcagc ttgagatccc ggtgagtact gtggaatttg
    11461 tggcgcagaa agtgttcaac aaggggatca gggttatcct caatcccgct ccggcaaggc
    11521 tgctgcccaa cgaactgctt gagtgccttt acctgatcac tccaaatgaa attgaggctg
    11581 aattacttac aggtatcaaa gtaaccgatg ccgcatctgc caggacagct gccgggaaac
    11641 ttctccataa aggcgtccgt aatgttgtaa taaccatggg tgcggcagga gcctttctta
    11701 tgaaccagga ttttgcaggc atggtgccgg ttgtacctgt gaaggcagtg gatacgaccg
    11761 cggcaggcga tgttttcaac ggggcgctgg cagttgcgat cgcggaaggc gcggaattga
    11821 aggatgctgt ggattttgcc aacagggccg cggcaatatc ggtcacaagg atgggggccc
    11881 agtcttcagc cccatacagg aaggaattac agtaaacact gaacgaagcc tctgctgggg
    11941 tttcatcaga acaaataaca cttttatccc gatcatgatg accaacaaaa tccttttcct
    12001 cattttcctg gtcctcaccc tcggcggatg cctgaacccg gcatccagga atgagaccgg
    12061 catcccgaaa ataatcttcg ataccgatct gggtcctgac tatgacgacg tgggtgccct
    12121 ggcctttctc cacgccatgg ccgacagcgg aaaagccgaa atcctggcaa ccatatcatc
    12181 aaataagcac gaacttgttg ctccttctat cgaaataata aatacatatt tcaaaaggcc
    12241 ggaacttgag ataggtgcac cgaaatcgga aggtgcaaat atgggatcaa cccagcactg
    12301 ggcagattcg ctggttgcaa actatccaca taaaataaga tcgactgatg aggtacagga
    12361 cgcagtatcg ctgtacagaa aaatcctgaa cagccagccc gacggcagtg ttacaattgt
    12421 cacagtcgga ttcctgacaa acctggccaa tcttctcaga tcagaggcgg atgctgtcag
    12481 ccccctctcc ggccgtgacc tggtttcaaa aaaggtaaag ctgctcgtgt caatggccgg
    12541 aaaattccct gaaggcaggg aattcaatgt ttacattgac tcattatcgt caaagtatgt
    12601 gttcgacaac tggcccggta aaattatatt caccggctgg gaaattggag tagaaatcag
    12661 gacgggcctg aaggtgatgc agctgccggt atctggcagc ccggtaaagg atgtgttcaa
    12721 aataagcata ccaatgtcgg aggaagacag gatgggaagg atgagctggg atgagactgc
    12781 cgttcttatc ggtgtttacg gaaccgacgg gtttttcgat actgtgaggg ggaaaatcat
    12841 tgtgaacacc gacggcagca acggatggac tgatgacccg gatggcaatc aattctacgt
    12901 ggttcagaaa atgcccgtac aggatatggc cgggttcata gaggaaagga ttatgcacat
    12961 tccgcttgga aagtaaatga ctcaggcttt ttgttcagaa tgtgacgcgt gggagaaaat
    13021 ccgactatca taaaataatc cgccgtgaaa acgggaaaac atgattggta aatgaattca
    13081 gatgtttccc gaaacagaaa agcaaaaaaa ggaaaagaac aaggttttac agaataaact
    13141 caactggtat gaaaaaaatt ttcggttttt tgtcagttat cctggtgctt ggcatgtttt
    13201 cctgcaagga atcaggcacc ggattcagga agatagatcc cgccgttttg aaggataaga
    13261 ttgccggcgg ctgggcaggc aaaatgattg gtgtcacata cggtgctccc acggaattca
    13321 aagccggggg aaagacctat gaagatccca taaactggaa accggaggat attaaaggaa
    13381 gcatatggca ggatgatata tatgtccagc ttacatttct tatgacaatg gataaatttg
    13441 ggatggatgc ttcgcagaaa cagttccagg agatgctcgc aaaggccggt tatcctttat
    13501 ggcatgccaa catgcaggcc cggaagaatt atctcgattc gatctttgct cccctgtcag
    13561 gaaacccgga atacaatatt catgccgatg acattgattt tcagattgag gccgactaca
    13621 ttgggttcat gtgtccagga atgccacgca cagcttcggg gattgccgac aagataggac
    13681 atatcatgaa ttacggcgac ggtgtgtacg ggggaatatt tgttgcagcg ctttatgcag
    13741 aggcttactt cgaaccggac atcaggaaga tcgtcaaaca ggccctgaga tcactccctg
    13801 aagaaagtga ctatcataag atagtggaag atgtgataaa gcttcacggg cactatcctt
    13861 ccgactggcg cgcagcctgg caggaacttg aaaataaatg gggtgatgtg gatatctgcg
    13921 gggcaggaac cgacttcaat atcgatgcaa aactcaatgg agcctacata gttatgggat
    13981 tactgtatgg tgacggtgat ccgtacaaaa cccttgaaat ctcaacacgc tgcggccagg
    14041 attcggactg caatccctcg aatgcactcg cggtcctcgg agttataaac ggtttcagcg
    14101 gactgccgca ggaaatgcag gatggtgtaa aggcaatcgg cgattcactt ttcattttta
    14161 caacctattc atttaattcg gcagtgcaaa gcacatacaa ttatgccctg gatcttattt
    14221 ccaaagaagg cggcagggta accggcaaca ggatccttat ccgtccgcag gaaccggtcc
    14281 ggccccggct tgaggttgcg tttcccaata tcctgtttga caagaagatc tcggtgtttg
    14341 aaaagggcgg tttcaggttc accggtgcat ggaaaccact cgaggtaaat tacggggccg
    14401 gcagcgtgcc cgttaggcaa tccatgtatt ctgacaggcc tggtgatgaa cttgtactgg
    14461 aattcagcgg aacgggtatc tccctcaccg ggaacctgta cagggacggc ggcaaggccg
    14521 acgtttatat cgacggaaag cttcacagga ccattgatac ctattattac tttggcaacc
    14581 agcagcagcc ggaggaaagc atgtggcaca taatgggcct cacccccggg gaacacaagg
    14641 tgcgcctggt ggttaaagga gaaaaacgac cggagtcctc cggaaccagg atttatgttt
    14701 catctgccaa catattcaga acaggatcaa agaaaaacga aaatttcagg ttctcatttg
    14761 agtaggctgc tgaagcttaa accgtgacag tatccataat gcccttccgt agtctggggt
    14821 aatcagccat tggaggatta ttttccggcc gtgtcttttt gtttgaacgg tgggcgcggc
    14881 tctttgcttt tggcttttta ccgctaaaat caattaaaag agaaaacaaa aacaatgtgc
    14941 tgcaagtgtg ccggagcaaa aagtctatct ggcggcagac aggcatgaca aaaaataagc
    15001 cggcagggca cggccgaggg ggagctgctc ccaaaaatgt gggaacaatt agaagatttt
    15061 tgggagctgc agggattatt gtttgtcggt cccggattgt ttatagccta tcgttttcct
    15121 gggtctttcc tggacatcaa taaattttct caaatactgg aggatcatag caatgttttc
    15181 atcctgcttg cctactcttt tttccagctg ttccagcttt agtaaaatat ccttatgaga
    15241 aagtaccaac tccctcattt taatatagat ctcaacgatc cgtatagatg cctctatcgc
    15301 cctgtcgctg ttcagaatat ttgcaagcat aacagatcca tgttctgtaa aagcataagg
    15361 taaatacggg gagtatttca ggttctggag gtggtcgcaa attacgacca tcttttcttt
    15421 ctctttttca gttagttgga acataaaatt gccaggaaag cgctttatgt ttcttttaat
    15481 tttttaaagc ctgggagcct gccccgctcc agcggggctt ttaagtcca
//
LOCUS       DAOOYG010000059        15506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3774_length_15506_cov_7.093457, whole genome shotgun sequence.
ACCESSION   DAOOYG010000059 DAOOYG010000000
VERSION     DAOOYG010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15506
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3774_length_15506_cov_7.093457"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="PK288_06650"
     CDS             complement(<1..1538)
                     /locus_tag="PK288_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPM18637.1"
                     /translation="MKDIIEVQGARVHNLQNIDVVIPRNKLVVITGLSGSGKSSLAFD
                     TIYAEGQRRYMETLSAYARQFLGGMERPDVDRITGLSPVISIEQKTTNKNPRSTVGTI
                     TEIYDFLRLLYARAGEAFSWASGEKMVKYTDEQVIELIRNQFDDRKVYFLAPVVKGRK
                     GHYKELFEQLRRKGFLHVRIDGEIREITHGLRLDRFKIHYIELVVDKFRVGSVNTKRL
                     TDSVLLSLDQGDGVMMLIDQENPEPRYFSRHLMCPVTGISYNEPAPHTFSFNSPQGAC
                     PHCNGLGEISGIDIKKLLPDPGLSIRKGGIEPLGKYKNSWIFWQIEAIAEKNGFTLDT
                     PVGEIPEDALNKVLYGSDEVLKLSNTPLGMTSNYFLTFEGVVNYIGNIEYINGKNNGT
                     RKRDQYVHYETCPECNGTRLKKEALFFRIAGKNIGELASMGIRELYAFLGEAEEKMTE
                     KQKLISAEVLKEIRSRLGFLLDVGLDYLSLNRGARTLSGGESQRIRLATQIGSKLVNV
                     LYIL"
     gene            complement(1564..2523)
                     /locus_tag="PK288_06655"
     CDS             complement(1564..2523)
                     /locus_tag="PK288_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPM18638.1"
                     /translation="MLSTERILTLALFVAIGFIFPGSASNRDTASLRKAEVNSVLPDT
                     IWYNTSFTIPDNVTFAGERMPLENFDTRESLERELLTSAYRHSSTIMIIKRAKRYLPV
                     IEKILEKNRVPDDFKYLVAAESEYMNVVSPKGATGFWQIMPGTGREAGLEISAEVDER
                     YHIEKSTQFACDFLLRSYEKYGNWTLAAASYNGGRAAIDEQIKIQKEKNYYDLLLAEE
                     TARYIFRVVAYKLIITDPQRYGFNIREDELYPELNCNIVKVDSAIQDISEFARKHGTN
                     YKILKFLNPWLRKPYFSPKPGKEYYIKLPVEGMRSIERIDSDY"
     gene            complement(2735..3061)
                     /locus_tag="PK288_06660"
     CDS             complement(2735..3061)
                     /locus_tag="PK288_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18639.1"
                     /translation="MPLILRYKLIDKDLDFNLIGGFSYNMLINNSVYTYVNGSKYQVG
                     ETEGLNPLMVSSSLGMGLEYNLSRKISLNLEPTFRYYLNPFSQTQAVKTHPYSFGLFS
                     GLSYRF"
     gene            complement(3007..4053)
                     /locus_tag="PK288_06665"
     CDS             complement(3007..4053)
                     /locus_tag="PK288_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18640.1"
                     /translation="MVEKKANIDLLFRKGLDDLEILPPVGTWNNIYPVIRKRQKPYML
                     LRSAALIAVLISISFLAYKWSREISTSLQNSFIVRDEESTRTRIDNFNPVIIRQEAVV
                     FNPAESHVRTTVPGIPEPEAVTARESNQEIIVVPVSANSYPGFNLAGHADNNMIRIAD
                     YQPVKDYSEPYGVIEVIPGNPAGNGKARWSLSAIASPSYYLKSVYGTDDISGQINSYE
                     KPRMSYSGGIGFAYKINDRLSVQSGLFYTSVGQEIENIYSYAGFRPYDNTKNHHNFEV
                     LTSGGMIYTDNRDVFLLDNGGERVITKFTNDVFDPAKQVFFISTTHCTRHSAILKCRL
                     FSGTNLLTRIWTLT"
     gene            complement(4046..4606)
                     /locus_tag="PK288_06670"
     CDS             complement(4046..4606)
                     /locus_tag="PK288_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPM18641.1"
                     /translation="MPETVNIIKGCIAGNRRDQELLYRRHAPRLYAVCLQYSDNDEEA
                     RDILQDGFIKIFENLINFKNEGSFEGWMRRIIVNTALEKYRSRHNLYRVDDIDLIPEP
                     DADPDNEDYSGLEAIDLLNIIRELPPKYRMVFNLYAIEGYAHKEISRMMKISEGTSKS
                     NLSRARLILQKKVSLYTGMNKKVVNG"
     gene            complement(4822..6213)
                     /gene="lpdA"
                     /locus_tag="PK288_06675"
     CDS             complement(4822..6213)
                     /gene="lpdA"
                     /locus_tag="PK288_06675"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPM18642.1"
                     /translation="MEYDLIVIGSGPGGYVASIRASQLKMKVAVIEKADIGGVCLNWG
                     CIPTKALLKSAQVYEYLKHPADYGITAEGSVKPDFGAIIARSRAVAEGMSKGVQYLFR
                     KNKIDQLTGKGRLAGNGMVEVVDAAGEKKQYSAKNIILATGARSKELPGLKQDGRKII
                     GYREAMSLEKQPESMVIVGSGAIGSEFACFYQTLGTSVTLVEFLPRIVPNEDEEVSKY
                     LERSFKKMGMKIMTGSSVESAEIAGEKCLARIRTPKGEETVETDIIFSAVGVTPNLED
                     LGLETAGVGTDRGKVIVDEYYRTPVPGIFAIGDIVHGPALAHVASAEGIICVEKIAGL
                     NPEPLDYRNIPACTYTSPEIASCGYTEAAAKEAGFDIKVGKFPFTASGKASAAGQKEG
                     FVKLVFDAAYGELLGAHMIGANVTEMISEIVVARKLETTAHELMKSVHPHPTMSEAIA
                     EAAAAAYDEVIHL"
     gene            complement(6488..7315)
                     /locus_tag="PK288_06680"
     CDS             complement(6488..7315)
                     /locus_tag="PK288_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPM18643.1"
                     /translation="MVEGSIFQYEFVIKGLLGAVFASITAGLAGTYIVSRRMVFLSGG
                     ITHASFGGIGIGYFAGISPVVGAAVFSVISALGVEYLSVRQKIREDSAIGILWAFGMA
                     IGIIFIYLTPGYSPNLLSYLFGSILTVTWADLFALGIMSVILIFYFGFFYRTILYISF
                     DEDFARTYSRYVNVFKYITTSLIALTIVLNIRMAGVVLVISLLTIPPNIAMLVSRNYF
                     TIVLLSVMAGFIGTATGYAISYYAGIPVGATIIFTLVVLWVLVKLMLKVINKIRTTS"
     gene            complement(7315..8040)
                     /locus_tag="PK288_06685"
     CDS             complement(7315..8040)
                     /locus_tag="PK288_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM18644.1"
                     /translation="MKSLSASYGAGLVLQDIDFRVCGNDFIGVIGPNGGGKTTLLKVI
                     LGLVRPVAGELIFSRELRNSNNIGYLPQISTGDSNYPVTVKDVVLSGLMIRKGLVSRM
                     NKADQKKADEVIEELGLESLAESSLNQLSGGQLQRVLLGRAIIGEPKLLLLDEPGNFV
                     DANFEHDFYEKLRDLNQRMAILMVSHDIGTISAHIKSFACVNRHLNYHPSSEISNEQL
                     LAYGCPIQLITHGAVPHTVLKTH"
     gene            complement(8074..8940)
                     /locus_tag="PK288_06690"
     CDS             complement(8074..8940)
                     /locus_tag="PK288_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPM18645.1"
                     /translation="MRNIPAFILLLLAVSCGKRGTVQEEKTLTVSIGPFKYFVEAIAG
                     NEYSVNVMVPPGSNPHIYEPYPDQISKLKKSAAYISNGFLGFEITWLDRFYEMNRKMK
                     TLSLGDVIDPIKPEHRHEREHLEGADPHYWVSPECAFKIAHSVKDFIAGLNPGNRDLY
                     EKRYEDLTARIKIIDSAMNELSSAPGRKEFIIYHPNLGYLARDYGFEEIPVEFEGKEP
                     SPARMKYLIDRARNNGLKVIMVQKEYDTRNARAIASETGAEVIVIDPLSEDWYSFELK
                     LIEVLKESFGQK"
     gene            9073..10740
                     /locus_tag="PK288_06695"
     CDS             9073..10740
                     /locus_tag="PK288_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPM18646.1"
                     /translation="MKMRLKTAFVFIILWLAILPLFSQYKYSDFSTLTRRINVLKDKY
                     PRLCTVKSLVKTAGGREIWVLTAGTGNMDEKPGIVICGGIEGNHLLGQEIAMGFAEKL
                     LENSSSQEIRNLLEKVTFYVFPNLSPDAAQQYFSDIRYERVANARPCDDDRDFLIDED
                     PFEDLNNDGLITLMRIYDPAGTHIENVRDNRLMVPADLSRGEKGKYLVYSEGIDNDDD
                     GLFNEDGEGGVNFNNNLTWKYEHFGKNAGLHPVSEPETKAVLDFLFDRFNVFAVFAFG
                     PQDNLGQPMKSPEPKSAGGEKGQDGDEGRWFMPRPVSLKSVLKSDELINIMVSDKYHE
                     ITGAKGAPVAAAAPGNFMEWAYYHYGRYSFSTPGWWFPIEKSETREEAFLKFAEKTGM
                     NDVFVPWSEISHPGFPGKKVEVGGIKPFVMINPPADTLGDLIEAHYRFLVAASLMHPE
                     LEFLDIKTENAGDNIFRLTVKVHNKGLFATCAEVGDESIWTRIMRIVITPARNQTIIS
                     GLKTQRIKRLQGNETAEFTWLISGKGSTGISAGAANTGFISTSIDLK"
     gene            10759..12486
                     /locus_tag="PK288_06700"
     CDS             10759..12486
                     /locus_tag="PK288_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPM18647.1"
                     /translation="MTRRIAIPILFLAISLSFQARAQNAELFFKAAGSPSNPKVSTSW
                     NKYFTYEGIMDFCRKLAKEHPDLVTLETAGKSYKGRDIIAIVISEKKGQSPDKKPGFY
                     IDGNIHSNEIQGSDMALYTAWYLCEMYKTNSFINQLLKDKVFYILPTINPDARQYFMD
                     EPNSTHSPRSGLVPFDNDRDGLYDEDGYDDLNNDGVISMMRRKNPDGKYITDPKDPRR
                     MIMVKPGEKGEYELLGLEGIDNDNDGLINEDGIGGYDPNRDFGFNWEPNIVQWGAGKY
                     PFSFPENQAVRDFSLKHRNIAGAQSFHNAGGMILRGPSAKDTGTEVFTRADEMVINTI
                     GKKGEKIIPGYSLLTIWKDLYPVYGGELDWWYGAIGSYIFSNELWTQRLMFNDTSNVD
                     EYEFERLLLFEDAFIPWKEADHPVYGKIEIGGFDKNFGRIHPGFLLESDAHRNAAFCI
                     YNAWTMPKLEISSINVRDLPGGLKEVTATIANTQLMPTHSGTNLKYKIDPPDYVYLDG
                     GKVIAGMIVTDIDRNITVEQKKNPQRIEVSNIEGYGRVHVRWIVKGGSRYGVRVESVK
                     GGHVSRQAG"
     gene            complement(12773..13495)
                     /locus_tag="PK288_06705"
     CDS             complement(12773..13495)
                     /locus_tag="PK288_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18648.1"
                     /translation="MKRSVLFLSVLFLVSATISFRPAAGQAKSKEDRERELRMQEEID
                     AHKKAITEQKKSKEIQEGNLENQGDDLDETLEKALEPLGDWKKYRYIFRDFDRFRNRP
                     FVLHDFNNPGAPFVFTPGSDFFPGKLLGPNVEKSSWELSRTIKEKSFSSDYTFDVDPS
                     SGTVIMAVNGDCRAGEIRIRITMPNGKTYSDIAIDEFGNLNWKKSFKVSDGENQDKTG
                     VWKFQVNASKATGYFRISLLTY"
     gene            13641..15044
                     /locus_tag="PK288_06710"
     CDS             13641..15044
                     /locus_tag="PK288_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPM18649.1"
                     /translation="MKARNTILLPGIISIIILICAQIIIVSGIWKQKDEMFNLRYRIY
                     SQDALEYMDRNWGTDGFDTARLLIGSYSAKTAPELAGITNDSLLEARKKDVLYFVTTV
                     LTREQDLSEFLSAYFESQGIEKEFSSKVKINYFELIYNDRRIPIFINQEQPGSGIQEA
                     GKLKHRGRPGSTSQILVKGDRSENNNFRLDFEYYIDIRDKQRLVLRETSFSLALSVFS
                     IFAVVALFMKTYRNLMMEKRLSSLKTDFINNMTHELKTPLSTITVAGKTLEMPQVIND
                     GKRIMETAKLIGKQSVHLNQLINMILEISMWERTQFEIEKKKVRIEETIREIVDCFRN
                     GSGQSASISETYDFSSAEMELDPVYFTTLINNLLSNAVKYSGDGPEIEVRGFTSGNNA
                     VIMISDNGIGISRTDQRHIFEKFYRAGSGNIHKYKGLGLGLYYVKKIAEAHGGNVTVS
                     SRTGKGSTFTVTIPYKI"
     gene            15058..>15506
                     /locus_tag="PK288_06715"
     CDS             15058..>15506
                     /locus_tag="PK288_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPM18650.1"
                     /translation="MKVLLAEDDRDFGNILSQYLTISGFEVILAKDGQEAITLFRSER
                     PDICVLDVMMPEMDGFSLGEKIKQIQPGLPVIFLTAKSLREDIIRGLKIGADDYITKP
                     FDPEILVLRINNILKRVYSAMNDEFSISDTVLKFNTLELISGGRREKL"
BASE COUNT         4290 a         3609 c         3380 g         4227 t
ORIGIN      
        1 tccaatatgt agaggacatt caccagtttt gatcctatct gagttgcaag cctgatcctc
       61 tggctttcac cacccgacaa tgttctggct cccctgttga gcgacagata atcaagtcca
      121 acatcaagta ggaaaccaag ccttgacctg atctccttga gtacctcggc agatatcagc
      181 ttttgctttt ccgtcatttt ctcctccgcc tccccaagaa atgcataaag ctcccgtatg
      241 cccatggaag caagttcacc aatatttttc ccggcgatcc tgaaaaaaag cgcttccttt
      301 ttcaacctgg taccattaca ttccggacat gtttcataat gaacatactg gtcacgtttc
      361 cgggtaccgt tatttttgcc attgatgtat tcaatgttac cgatgtaatt aacgacaccc
      421 tcaaaagtca ggaaataatt tgaggtcata cccagggggg tattggatag tttaaggacc
      481 tcatccgatc cgtacagcac cttgttcagt gcgtcttcag gaatttcccc gacaggagtg
      541 tcgagtgtaa aaccgttttt ttcggcaatt gcctcgatct gccagaagat ccaggaattt
      601 ttatattttc ccaggggttc aatgccgcct tttcgtatgc tgaggccggg atcaggtaaa
      661 agctttttaa tgtcaatgcc ggaaatctca cccagcccgt tacaatgcgg acaggcgccc
      721 tgcggggaat tgaatgagaa tgtatgggga gccggttcat tgtatgatat accggtcacc
      781 gggcacatca gatggcggct gaagtatcgt ggttcgggat tctcctggtc tatcagcatc
      841 attacaccgt cgccctgatc aagcgacaat agaaccgaat cagtcagacg ttttgtattt
      901 acgctcccta ccctgaattt gtcgacaaca agttcaatat aatgaatctt gaaccggtcg
      961 agccttaaac catgggtaat ctccctgatc tctccgtcaa tcctcacgtg aagaaagcct
     1021 tttcgcctca actgctcaaa aagttccttg taatgacctt tccttccttt tactacgggg
     1081 gcaagaaaat aaaccttgcg gtcgtcaaac tgattcctga taagctcgat cacctgctca
     1141 tcggtgtatt taaccatctt ctctcccgat gcccatgaga aggcttcccc ggcccgggca
     1201 tacaacaagc ggagaaaatc atagatttcg gttattgtcc caaccgtcga cctggggttt
     1261 ttatttgtgg ttttctgttc tattgatatt accggactca atccggttat cctgtctacg
     1321 tcgggtcgtt ccatacctcc cagaaactgc cgggcatatg ccgaaagagt ttccatgtag
     1381 cgcctctgcc cctcagcgta gattgtatca aaagccagtg atgatttccc gctcccgctg
     1441 agccctgtta ttacaacaag cttattccgc ggaatcacca catcaatatt ctggagatta
     1501 tggaccctgg ctccctgtac ctcaataata tccttcattt cacatcctta atcgctcaag
     1561 atttcagtaa tctgaatcaa ttctttcaat tgaacgcatt ccttcaaccg gaagctttat
     1621 atagtattcc ttaccgggct ttggtgaaaa atacggtttg cgtagccatg gattgagaaa
     1681 cttcagaatc ttgtaattgg ttccgtgctt cctcgcaaat tcagaaatat cctgtattgc
     1741 agaatcaacc ttaacaatat tgcaattcag ttccggatac agttcatctt ccctgatgtt
     1801 aaatccatag cgctggggat ctgtaatgat aagcttgtag gccaccactc tgaaaatata
     1861 cctagcagtt tcctcagcca gcagaagatc atagtaattc ttctccttct ggatttttat
     1921 ctgttcatcg attgcagccc tgccaccgtt gtatgaagca gctgcaagtg tccagtttcc
     1981 gtacttctca tacgacctga gaaggaaatc gcatgcaaac tgcgttgatt tctctatatg
     2041 gtaacgttca tcaacctcag cgcttatttc cagcccggct tccctgccgg ttcccggcat
     2101 aatctgccag aaacccgttg cacctttggg tgataccaca ttcatatatt cactttctgc
     2161 tgcaacaaga tacttgaaat catccggcac cctgttcttt tcaagtatct tttcaataac
     2221 gggaaggtat cgtttggccc tctttataat cataatagtg gatgaatgcc tgtaagcact
     2281 ggtcaacaat tccctttcaa ggctttcccg ggtatcgaaa ttctcaagag gcattctttc
     2341 tcccgcgaaa gtcacattgt caggaatggt aaaggatgtg ttataccata tcgtatcagg
     2401 aaggacacta tttacttcag ccttcctgag actggcagta tcgcggtttg aggcagatcc
     2461 cggaaagata aaaccaattg caacgaacaa ggctaatgtt aatatccttt cagtgcttaa
     2521 catgaatttt ctttcttctt ctgtactggc caattgttaa atacctatgc aaataattaa
     2581 tatacagata caaagtattt atacatccgt ttattttccc ataaggggga aataaagtgc
     2641 aaaagtaaca tttttaaccc ttataataat gcagggacaa tgttaaagaa ataaaataca
     2701 ggggtgggtt atggtaagaa ttggggttca ggatctaaaa ccggtatgag agtccagaga
     2761 acaaaccgaa ggaatatgga tgggttttaa cagcctgtgt ctggctgaaa ggattaagat
     2821 aatatctgaa tgtaggttca agattgagtg atatcttcct ggacagattg tattccaggc
     2881 ccattccgag agaactgctg accataagcg ggttcagtcc ctcagtttcg cccacctggt
     2941 atttactgcc gtttacatat gtatatactg aattattgat cagcatgtta tacgagaaac
     3001 ccccaatcag gttaaagtcc agatccttgt caataagttt gtacctgaga ataagcggca
     3061 tttcaagata gctgaatgcc tggtgcaatg tgttgttgag ataaagaaga cttgcttggc
     3121 cggatcaaaa acgtcattgg taaattttgt gatgaccctt tctcctccgt tgtcaagaag
     3181 gaaaacatcc ctgttatccg tatagatcat tcctcctgag gtaagcacct cgaaattatg
     3241 atgattcttg gtattgtcat agggcctgaa accagcgtaa gaataaatat tttcaatttc
     3301 ctgcccgact gatgtataaa acagcccgga ctggacactc agcctgtcat ttatcttata
     3361 ggcgaatccg atacctcccg aataggacat cctgggtttt tcataggaat taatctggcc
     3421 tgagatatcg tcggtcccgt acacggattt caaataatag gatggtgatg caatcgcaga
     3481 cagcgaccat ctggcctttc cgtttccggc aggattgcct ggtataacct caatgacacc
     3541 gtaaggctca ctgtaatctt tgacaggctg ataatcagca atcctgatca tattattatc
     3601 tgcatgcccg gccaggttga atcccgggta actgttggcg ctgaccggaa caacgattat
     3661 ttcctggttg ctttcacgtg ccgtgacggc ttcaggttcg ggaatgccag gaacggtggt
     3721 cctgacatga ctttccgccg gattaaatac gactgcttcc tgcctgatta ttaccggatt
     3781 gaagttatcg atacgggttc ttgtggattc ttcatcacgt acaatgaaag aattttgcag
     3841 tgaggttgat atctctctgc tccatttgta ggccaggaag ctgattgata tcagcacggc
     3901 tatcaatgct gcacttcgta acagcatgta tggtttctgc ctctttcgta tgaccggata
     3961 gatattgttc caggtgccaa ccgggggaag gatctcaaga tcatcgagtc ccttcctgaa
     4021 aaggagatca atatttgctt tcttttcaac cattgaccac ttttttattc attccggtgt
     4081 ataaactcac ttttttctgc aggatcagcc ttgcccttga aagattggat tttgaagttc
     4141 cctcggagat cttcatcatt ctgcttatct ccttatgtgc atatccttca atggcataaa
     4201 ggttaaaaac catcctgtac ttgggtggca gctcccttat tatattgagc agatcaattg
     4261 cctcaagccc tgaataatcc tcattgtcgg gatccgcatc aggctcaggg atcagatcaa
     4321 tgtcatcaac cctgtagaga ttatgcctgc tgcggtattt ttccaatgcg gtattgacaa
     4381 ttattctgcg catccagcct tcaaaagaac cttcattctt aaaattgatg agattctcaa
     4441 agatctttat gaaaccgtcc tggagaatat cccttgcttc ctcatcgttg tccgaatact
     4501 gaaggcacac ggcataaagc cttggagcat gcctcctgta aaggagctcc tggtccctcc
     4561 ggtttccggc aatgcatcct ttaattatgt ttacagtttc aggcaaggtt ttttacctat
     4621 taaacatata aaaataatta aaattcatct gaattgccag aattatttta aaagaatgat
     4681 gcatttttga tcaaattggt tgcgtctatt attttttaaa caaaaatgcc ggcccgtgaa
     4741 ttgcagcagg cagctgtcag tttttcatcc ggcagcactt taccagctgt tgcaccagtc
     4801 cctccagcgg ctttcagtat ctcacagatg tatcacttca tcgtaggctg ctgctgctgc
     4861 ctctgcaatt gcttccgaca ttgtgggatg aggatgaacc gatttcataa gctcgtgtgc
     4921 ggttgtttcg agttttcttg caacgactat ctcagaaatc atttctgtaa cattagcgcc
     4981 gatcatatga gctccaagca attcaccgta ggcagcatca aacacgagct tcacaaaacc
     5041 ttctttttgt ccggcggcgc tggctttccc cgaggctgtg aaagggaatt tcccaacctt
     5101 gatatcaaag cccgcttctt tggccgccgc ctcagtgtaa ccgcaggatg caatctcagg
     5161 gctggtatat gtacaggcag gaatgttcct gtaatcgagt ggttcgggat tgagccccgc
     5221 aatcttttca acacagatga ttccttcggc tgaggcaaca tgtgcaaggg ccggaccatg
     5281 tacaatgtcg ccgatggcaa atattcccgg taccggggtc ctgtaatact catccacaat
     5341 aacttttccc ctgtccgttc cgacgcctgc ggtttcaagt cccagatcct ccagattcgg
     5401 ggtaaccccg accgctgaga aaatgatatc cgtttcaacc gtctcttcac ctttgggggt
     5461 cctgatcctg gccagacatt tttcaccggc aatttcagcc gattccactg aagagccagt
     5521 catgatcttc attcccatct tcttgaacga cctctcaagg tattttgaaa cttcttcatc
     5581 ttcattgggt actattctcg gaaggaactc cacaagggtt actgaggttc cgagtgtctg
     5641 gtaaaaacag gcgaactcgc tcccgattgc accagacccg actatgacca tcgattccgg
     5701 ctgtttttca agcgacatgg cctccctgta tccaattatc ttccggccat cctgcttcag
     5761 ccccggaagc tctttcgacc ttgcccctgt cgcaaggata atgttctttg ctgagtattg
     5821 ctttttttcc cctgccgcat ccaccacttc aaccatgcca tttccggcaa gacgcccctt
     5881 tccggtgagc tgatctatct tattcttcct gaaaagatac tgtacaccct tgctcatccc
     5941 ttctgccact gccctgctcc tggctattat cgccccaaaa tccggcttca ccgatccttc
     6001 tgcagttata ccgtaatcag ccggatgctt cagatactca tacacctggg cactcttcag
     6061 caatgcttta gtgggtatgc aaccccagtt caggcagaca cctccgatat cggccttctc
     6121 aataacagcc actttcatct taagttgtga agccctgatt gaggcaacat aaccgccggg
     6181 cccgcttcct ataacaataa ggtcatattc cattttaatc tgtttttagt cagtgaaaaa
     6241 aatcatttcc cgttatttaa actatattga tttacatcat atttcttact gattttgagc
     6301 tatttaatta ttgatatatg tcattacatc aagcaatatt tatcaatatc gcagtcattc
     6361 tcctgactga ttccaggata ttgaggccgg ccggtggttt catatccgtc agttaatagc
     6421 agtaatgaaa acaacgccag gataacttct ctgcaaaccg gcctgctgta agtcagaatt
     6481 gttgatatca tgatgtcgtt ctgattttat taatgacctt caacatgagc ttgaccaaca
     6541 cccacaaaac aacaagtgtg aatattattg tagcgccgac cggaatacct gcataatatg
     6601 aaatggcata acctgttgct gttccaataa agcctgccat aaccgaaagg agcacgatgg
     6661 taaaatagtt tcttgaaaca agcatggcga tattcggtgg aatggtcagc agggatatta
     6721 ccagcaccac gcctgccatt ctgatattca gaacaatggt aagggcgatg agggaagtag
     6781 ttatgtattt gaacacattc acgtatcttg aatatgttcg ggcaaaatcc tcatcgaatg
     6841 agatataaag tatggtccgg taaaagaacc cgaaataaaa gatcaatatc accgacatta
     6901 ttccaagggc aaacaggtcc gcccaggtta ccgtcaggat gcttccgaac aggtaactta
     6961 gcaggttagg ggaatatccc ggagtgagat agatgaatat tataccaatg gccattccga
     7021 aagcccagag aattccaatt gctgaatctt ctcttatttt ttgcctcact gacaggtatt
     7081 caacgccaag tgcggaaatg acactaaaga cggctgcgcc gacaacagga ctgatgcctg
     7141 caaaatatcc tatcccgatt ccgccgaaag atgcatgggt aatacctccg ctcaggaaaa
     7201 ccattcgcct ggaaacgata taggttcctg caagtcctgc agttatgctt gcaaatacag
     7261 ctcccagcaa acccttgata acaaactcat attgaaaaat actcccttcc accatcaatg
     7321 agttttaagg accgtatgcg gaaccgcacc gtgagtaata agctggatgg gacaaccata
     7381 tgcaagcagc tgttcatttg atatttcaga tgagggatgg taattaagat gccggttcac
     7441 acacgcaaat gattttatgt gagcagaaat ggttcctatg tcatgtgaaa ccataagaat
     7501 ggccatccgc tggtttagat ccctcagctt ttcatagaaa tcatgttcaa aattggcatc
     7561 gacaaaattt ccgggttcat cgagaagcag gagtttgggc tcgcctataa tggcccgtcc
     7621 caggaggaca cgctgcagct ggccgcctga aagctggtta agcgacgatt ctgccaggct
     7681 ttccaggccc agctcctcaa taacttcgtc tgctttcttc tggtcagcct tattcattct
     7741 tgaaacaaga ccctttctga tcatcagtcc ggataataca acatccttca cggtaaccgg
     7801 gtagttggaa tctcctgtag agatctgcgg caggtaacct atattgttgc tgttcctgag
     7861 ctcgcggctg aagatgagtt ctcccgccac aggtctgacc agacccagga taactttcag
     7921 cagggtggtt ttccctcctc cgttgggtcc gataaccccg ataaaatcat ttccgcaaac
     7981 cctgaaatca atatcctgaa gtaccaggcc agcgccatag gaggctgaaa gtgatttcat
     8041 ttcaaacaaa caggttgcca tacacaagtc agcttatttc tgcccgaaac tttcttttaa
     8101 tacttcaatc agcttcagtt caaatgaata ccaatcttct gacaaaggat cgatcactat
     8161 gacctctgct ccggtttcag aagcaatcgc cctggcattc cttgtatcat attccttctg
     8221 aaccattatt actttcagtc cattgtttct tgcacgatcg atcaggtatt tcatcctggc
     8281 cggcgagggt tccttacctt caaactcaac cggtatttcc tcaaatccat aatcacgggc
     8341 aaggtaacca aggttcggat gataaataat gaattctttc cttcccggtg ccgaggaaag
     8401 ctcattcatt gccgaatcaa taatcttaat ccttgcagta aggtcttcat atcttttttc
     8461 ataaaggtcc ctgtttcccg gattgaggcc tgctataaag tccttcaccg aatgtgcaat
     8521 tttgaatgca cattcgggtg acacccaata gtgaggatcc gcaccttcca gatgttccct
     8581 ttcatgccgg tgttccggtt ttatgggatc aattacatct ccgagcgaaa gggttttcat
     8641 ctttctgttc atttcataaa accggtcaag ccaggttatt tcaaaaccca ggaaaccgtt
     8701 gcttatatag gcagccgatt ttttcagttt gctgatctgg tcggggtaag gctcatatat
     8761 atgtggattt gagcctggcg gcaccatgac attgacactg tattcattcc ctgctatcgc
     8821 ctcaacaaag tatttgaaag ggcctatact gactgtcagt gttttctcct cctgcacggt
     8881 tcctcttttt ccgcatgata cagcaagcag caggaggata aaggcaggaa tatttctcat
     8941 cagccgggca tttacgttaa ttacaaattt aacatttaac ctgataaaag gtttaggtgc
     9001 cggaaataaa ataatatttg tagttttgga tcttattatg acaaataacc gcctgattaa
     9061 atcaagaaaa taatgaaaat gcgattaaaa actgctttcg tttttataat tctctggctg
     9121 gcaatactgc ctctgttttc ccaatacaag tactccgact tcagtaccct tacccggcgt
     9181 attaatgttc tgaaggataa atatccccgg ctttgtacgg tgaaatcgct cgtaaaaacg
     9241 gccggaggaa gggagatatg ggttctgacc gccggcaccg gcaacatgga cgaaaaaccc
     9301 ggcattgtta tctgcggcgg aattgagggg aatcatctcc tgggtcagga aattgccatg
     9361 ggatttgcgg agaagcttct tgagaactcg tcttcacagg agatcaggaa tctcctggaa
     9421 aaggttactt tttatgtatt tccaaacctt tcacccgatg ccgcacaaca gtacttttcg
     9481 gacatcaggt atgagagggt tgcaaatgcc agaccctgcg acgatgaccg ggattttctc
     9541 atagacgagg atccctttga ggatcttaat aacgacggtc tgataaccct gatgaggata
     9601 tacgatcctg ccggcacaca tattgagaat gtcagggata acaggctgat ggtcccggcc
     9661 gacctttcca ggggggaaaa aggcaaatat cttgtttaca gcgaagggat agacaacgac
     9721 gatgatggac tcttcaacga agatggtgaa ggcggagtaa atttcaataa caacctgacc
     9781 tggaaatacg agcatttcgg gaaaaacgcc ggactgcatc cggtgtcgga gcctgaaaca
     9841 aaagctgttc ttgattttct ttttgacagg ttcaatgttt ttgccgtttt cgccttcgga
     9901 ccgcaggata atctcggtca gcccatgaaa tcgcctgaac cgaagtctgc cggcggggaa
     9961 aaaggtcagg atggtgatga aggaagatgg ttcatgccaa ggccggtatc tctgaagtca
    10021 gttctgaaat cagatgagct gattaacata atggtctcag acaagtacca tgagataacc
    10081 ggggccaaag gagcgcctgt tgccgcagca gctccgggga atttcatgga atgggcatat
    10141 tatcattatg ggcgctatag cttcagcacc cccggatggt ggtttcccat tgaaaaatca
    10201 gaaacacgtg aagaggcatt cctgaaattt gcagaaaaga ccggcatgaa tgatgtgttt
    10261 gttccctggt cggagatcag tcaccccgga tttcccggca aaaaggtgga agtaggaggc
    10321 atcaagcctt ttgtgatgat caatcctccg gctgacacat tgggtgatct tattgaggcg
    10381 cactacaggt tcctggtagc tgcatcgctg atgcacccgg aactggaatt ccttgatatc
    10441 aaaacagaaa atgccggtga caatattttc agacttactg tgaaagtgca taataaaggg
    10501 ctttttgcca catgtgccga agtaggtgat gagagtatat ggacccggat aatgaggatc
    10561 gtcattaccc ccgccaggaa ccagaccatc atcagcggat tgaaaaccca gaggataaaa
    10621 cggttacagg gaaatgaaac agccgaattc acctggctca tttcaggtaa aggcagtaca
    10681 ggaatcagcg ccggcgccgc caacacagga ttcatcagta catcaattga cttaaagtaa
    10741 catatcaaaa taaagataat gacaagaaga atagcaatcc cgatattgtt cctggcaatt
    10801 tccctgtcat tccaggccag ggcccagaat gccgaactgt ttttcaaggc agcaggatca
    10861 ccatccaatc cgaaagtatc aacatcatgg aataaatact tcacatatga aggcatcatg
    10921 gatttctgcc ggaagcttgc caaggagcat cccgatctgg tcacgcttga gactgccggc
    10981 aaatcatata agggaaggga tattattgcc attgtgatat cggaaaagaa agggcagagc
    11041 cctgacaaga agcccggatt ctatattgac ggaaacattc attcaaatga aatccaggga
    11101 tcggatatgg ctctttatac cgcatggtac ctgtgtgaaa tgtacaaaac caactcattc
    11161 atcaatcagt tgctgaaaga caaggttttc tacatcctgc caacgatcaa ccccgatgcc
    11221 agacaatatt tcatggatga gcccaactcc acccattctc cgcgttcagg cctcgtcccg
    11281 ttcgacaatg accgcgacgg actgtatgat gaggacgggt atgatgattt gaacaacgac
    11341 ggagtcatca gcatgatgcg cagaaagaat cccgacggaa agtatattac cgacccgaag
    11401 gatccgcgaa ggatgatcat ggtgaagccg ggtgaaaagg gagaatatga gctgcttggc
    11461 cttgaaggca ttgacaacga caatgacgga ctgatcaacg aagacggcat cggcggttac
    11521 gatcccaacc gcgatttcgg attcaactgg gaacccaaca ttgtgcagtg gggtgcagga
    11581 aagtacccgt tttcatttcc tgaaaaccag gctgtaaggg acttcagcct gaagcatcgg
    11641 aatattgcag gtgcacagtc atttcataat gccggtggta tgatcctgag gggtccgtcg
    11701 gcgaaggata ccggtactga ggttttcacc agggccgatg agatggtaat caataccatc
    11761 gggaagaaag gtgaaaaaat catccccgga tacagtctgc tgaccatctg gaaggatctg
    11821 tacccggtat acggaggtga gctcgactgg tggtatggtg caatagggag ctatatcttt
    11881 tccaatgagc tgtggaccca gaggttgatg ttcaacgaca catccaacgt cgatgaatat
    11941 gaattcgaaa ggctccttct ctttgaagac gcgtttatcc catggaagga agccgatcat
    12001 cctgtttacg gtaagatcga gatcggcgga ttcgacaaaa atttcggacg gatccatccc
    12061 ggatttcttc ttgaaagcga tgcacaccgg aatgctgcgt tctgtatcta caatgcatgg
    12121 accatgccca aactggagat ttcatcgata aatgtcaggg atctgccggg cggcctgaaa
    12181 gaggtaactg caactattgc gaatacccag cttatgccga cgcattcggg aaccaacctt
    12241 aaatacaaaa tcgatcctcc cgattatgta tatctcgatg gcggcaaagt aattgccggg
    12301 atgatcgtaa cggacattga ccgcaatatt acagttgagc aaaagaagaa tccgcagcgt
    12361 attgaggtct cgaatattga aggatatggc agggttcacg tcaggtggat cgtaaagggc
    12421 ggcagcagat atggcgtcag ggtggaaagc gtcaagggag gacacgtttc ccgacaggcc
    12481 ggataaaccc aaagaccata cctgatgctg tctttgaaag actgcctgta aagaaaggtt
    12541 tttaccgaaa tctctccaaa ggaggatgac agcagccgga tctcaggttc ccggaactgt
    12601 cacggggact ccgggaatat aaggtgataa cccctgcagc cctgaattat tcagacagaa
    12661 gcttaccctc gtacaaaaat cgggcgaaat aaagaaatga tacaggtatc caccagggcg
    12721 gaaaaaaaga agatgacccg ttgagagtcc ttccgcctgg cggcaataaa tactaataag
    12781 tcagaagcga tattctgaaa tatcccgtgg ccttgctggc atttacctga aatttccaga
    12841 cccctgtctt atcctggttt tccccgtcag aaactttaaa cgacttcttc cagttaagat
    12901 ttccaaactc atcgattgca atatcagaat aagttttgcc gtttggcatt gttatcctga
    12961 tccgtatctc tcctgcccgg cagtcgccat ttaccgccat tataaccgta cccgatgacg
    13021 ggtcgacatc aaatgtataa tcgcttgaaa aggatttttc cttaatggtc ctggataatt
    13081 cccacgagct tttttcaaca ttcgggccaa gcagtttacc ggggaagaaa tcgcttccag
    13141 gggtaaaaac aaagggagct ccgggattat taaagtcgtg caatacgaag ggcctgtttc
    13201 tgaaacgatc aaaatccctg aatatatatc tgtatttctt ccaatcccct aacggttcca
    13261 gggccttttc cagggtctca tccaaatcat caccctgatt ttccaaattc ccctcctgaa
    13321 tctcctttga ctttttctgc tcggttattg cctttttatg ggcatctatc tcttcctgca
    13381 tcctgagttc ccgctccctg tcctccttcg acttagcctg tcccgctgcc ggccggaatg
    13441 aaatggttgc agataccagg aataatactg ataaaaacaa aactgatctt ttcatggctg
    13501 ccttttttct gtttcagttt caaatttaaa aaccggaatc ctgccgcatt gttaatgcgg
    13561 ggttaatgtc aggttaatgt ttattcagta tgtccggggt aattgtaatt tgaataattt
    13621 taagaatcaa atcaatactg atgaaagcca ggaacaccat acttcttccc ggaattatat
    13681 ccattataat actgatatgt gcccagatca tcattgtaag cggcatctgg aagcaaaagg
    13741 acgaaatgtt caatttaagg tacaggattt actcacagga cgcacttgaa tacatggaca
    13801 ggaactgggg tacggacggt tttgatactg cccggcttct tataggcagc tattcggcaa
    13861 aaacagcacc tgaacttgca ggaataacaa atgattcact tctcgaggca agaaaaaaag
    13921 atgtattgta ttttgtcacc acagttctta cccgggagca ggatctttcc gaattccttt
    13981 ctgcttactt tgaaagtcag ggaattgaga aggaattcag ttccaaagtc aagatcaact
    14041 attttgaact catttacaac gacaggagga taccgatatt cataaatcag gagcagccgg
    14101 gcagcggaat tcaggaagca ggtaaactga aacaccgggg caggcctgga agcacttcac
    14161 agatactggt taaaggagac agatcggaaa ataacaactt ccggcttgat tttgaatact
    14221 acattgacat aagggacaaa cagaggctcg ttctcagaga gacttcattc tccctcgcac
    14281 ttagcgtatt cagtattttt gcagtagtgg ctcttttcat gaaaacctac aggaacctga
    14341 tgatggagaa aaggctttca agcctgaaga ccgatttcat caacaatatg acacatgaac
    14401 tcaaaactcc tctttcaaca atcaccgttg ccggtaagac tcttgaaatg cctcaggtca
    14461 tcaatgacgg gaaaaggatc atggaaaccg caaaacttat cggaaagcag agtgttcatc
    14521 tcaaccagct gataaacatg atacttgaaa taagcatgtg ggaaaggact cagtttgaaa
    14581 ttgaaaaaaa gaaagtaagg attgaggaaa cgatcaggga aattgtggat tgtttcagaa
    14641 acggcagcgg tcagagtgct tcaataagcg aaacctatga tttcagcagc gccgaaatgg
    14701 aactggatcc ggtttacttt acaactctca tcaataatct tctttcgaat gctgttaaat
    14761 attcgggtga tggcccggaa atagaggtca ggggatttac cagcggcaat aatgccgtca
    14821 tcatgatttc cgacaacggc ataggtatct caagaaccga tcagaggcac atttttgaga
    14881 agttctacag ggccggttcc ggtaacattc ataaatacaa ggggctcggc ctcggacttt
    14941 attatgtaaa aaagatcgct gaagctcatg gtggcaatgt aacagtaagc agtagaaccg
    15001 gaaagggcag tactttcacg gtaacaattc catataaaat ctaaaacatt tctcctgatg
    15061 aaagtattac ttgccgagga tgacagggac tttggaaaca tactgtcaca ataccttaca
    15121 ataagcggct ttgaagtcat cctggcaaag gatggacagg aagcaattac tcttttcaga
    15181 agtgagaggc ccgatatctg cgtgctcgac gtaatgatgc cggaaatgga cggattttct
    15241 cttggagaga agataaaaca gatccagccg gggctgcctg ttatattcct cacggcaaaa
    15301 agcctgaggg aagatatcat aagaggtctg aagatcgggg ccgatgacta tataaccaag
    15361 ccatttgatc ctgaaatact tgtactgagg atcaataata tcctgaaaag ggtgtattca
    15421 gcaatgaatg atgaattcag catatccgac acggttttga agttcaatac tcttgaactg
    15481 atctcgggag gcaggcgtga aaagct
//
LOCUS       DAOOYG010000060        15497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3780_length_15497_cov_7.326512, whole genome shotgun sequence.
ACCESSION   DAOOYG010000060 DAOOYG010000000
VERSION     DAOOYG010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15497
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3780_length_15497_cov_7.326512"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PK288_06720"
     CDS             complement(<1..302)
                     /locus_tag="PK288_06720"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPM18651.1"
                     /translation="MKKILVSMFALLTLAGMISGQRSASTITGRITGNGGMPLAGASV
                     IIVETGTGVAADNDGRFALRNLRDGYYNLRVSFTGYDPVDTLVKLNDLAVIDIALS"
     regulatory      complement(368..464)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            560..982
                     /locus_tag="PK288_06725"
     CDS             560..982
                     /locus_tag="PK288_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18652.1"
                     /translation="MKTLLDKLNYRGQLRIAIINPESNFKLAPSRILTSIQIDDVIDQ
                     RFPYGFMMIFVREPKDIEKYAPLALHNLATDGLLWFCYPKKTSNKYCPGIDRNHGWKV
                     LNEMDFYGTRVVTIDDDWAALRFRNKKFIKSRADKSSK"
     gene            1076..1606
                     /locus_tag="PK288_06730"
     CDS             1076..1606
                     /locus_tag="PK288_06730"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF014870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPM18653.1"
                     /translation="MIKIATIETPIGEMTVAAIKEGICMLEFTEARKPGADYAKLRQI
                     FNMDADFGTNRHTRALKKQLKEYFSGKRKEFSVPLFYHGSDFQKSVWRILEKIPYGKT
                     ISYMQLAEMLNNPKAIRAAANATGSNPLAIIIPCHRVVGSDGELTGYRGGIERKSWLI
                     DHERRFSGQPVDGILF"
     gene            1628..1984
                     /locus_tag="PK288_06735"
     CDS             1628..1984
                     /locus_tag="PK288_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18654.1"
                     /translation="MNSSLKNHYSFLFLSLLLFIILAGCARSEPVDHCLTGHTYGFFG
                     GLWHGFIAPFDLIGMLFNKKITMYAQNNNGGFYALGFLLGSGGWGFFGSRGSRRIYHR
                     KISVKSDRFDEAQIVE"
     gene            2068..2889
                     /locus_tag="PK288_06740"
     CDS             2068..2889
                     /locus_tag="PK288_06740"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM18655.1"
                     /translation="MDNIEKIINIRSAFRNSNSVFLRSIPGFFVTILEKLIFQHEMNA
                     TIHRSRDKEGVPFINDVLEGWNVKVKIIGGENVPAAGRFIFAGNHPVGGIDALAFYST
                     IYKFFPDVISPTNELLNQIPNLRPVMLGINVFGKNTRENAARIDELFESDSQIMIFPS
                     GEVSRKIKGVISDPVWQKTFVTKAIRHKRDIIPVHISGRNSGFFYFVASLRKFLGVRM
                     YLETILLPREMMKQRNSEVTITIGKPVSHLSLTKDKTHYEWAQEIRKAVYNLNIS"
     gene            2938..3819
                     /locus_tag="PK288_06745"
     CDS             2938..3819
                     /locus_tag="PK288_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HPM18656.1"
                     /translation="MTNIRNIEAIIPPPPYHMVGDGFKVHNFFPSSRIIGDTGMSPFF
                     LMDYNAKWEITPSERPRGVGVHPHRGIETITIAYHGRIAHHDSAGNSGVIGEGDVQWM
                     TAGGGVLHKEYHEKEFSRKGGLFQMVQLWISLPKRFKMTPPKYQTIESKDMTRVMLKD
                     GVSHVDLIAGSYMEKKGPASTFTPTNLFNASLKKGIRADFSFPLEYNTGLLVIEGEIN
                     INGSRNATADNFVYFSHDGEKFTVEAVTDSIVLLLSGEPVNEEIYPYGPFVMNTRDEI
                     KQAYEDFYNGKFGHLED"
     gene            complement(3901..4860)
                     /locus_tag="PK288_06750"
     CDS             complement(3901..4860)
                     /locus_tag="PK288_06750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPM18657.1"
                     /translation="MKTKIPRRRFIRVAAVTAAVGTIPVLGSSAAVAATSESAVKAEG
                     LPKRRLGRTNRMVSCIGFGGGSRYTNWIAPDEILQKHIDHAIKLGITYFDSARSYGDG
                     LCEERYGKFLTPKYREQIFLNSKSGERTYDGVMRDIEKSLSLLKTDYLDLYCMHAIDT
                     MEDVNTLLGQSGGYKAFLKLRNDGVVKNIGFSFHKWNEASQKSFREFDIDAVMCVINA
                     ARSNGNEDNLIPLAIERNLGIIAIKIMGQNALIGNVSGNDLLRFALSLPVTVANVGMD
                     GFAPLESCVEIGKRPIISAREAEEIVKNLDFDPKVTRLPYFTG"
     gene            complement(4899..7379)
                     /locus_tag="PK288_06755"
     CDS             complement(4899..7379)
                     /locus_tag="PK288_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPM18658.1"
                     /translation="MKKTDLFKLFVIVSFLGLLTGCSGDKGSDRLIINDQEYLEKPGL
                     SVLAFHNYYTVGHQGGIEIIQHGERIATNGFIRLRPADERRLPGPDRAEREADRQKQE
                     IVSSISYDDFDLNYKVRLKPEGNGFKLIVDLEKPVPAEWENRLSFEMEVYPPLYYGKT
                     FQIGETFGIIPRMGVRSVFTDEDGRISPVNMGQGKILTLAGEDPLRKIVFENKKGEII
                     LTDNRESSKSGWIMVRSVISPGVTQGALEWIIRPNVVNGWIREPVIAVSQVGYHPDQV
                     KQAVIELDRKTGKPEKARLMKVSGGKVREVLSGSPKPWGDFLCYKYAVFDFSAVKEPG
                     MYFVSYGTKSTNPFSIDQDVYRNGVWQPTLEGYFPVQMCHVKVRDGQAIWHGACHLDD
                     ALQAPLDIEHVDGYRQYKEAETSYRPLTTVPFQNRGGWHDAGDDDLAAGSQAATTHYL
                     VLANEICNNQTDQTSVDFQGLYVEMHKPDGIPDFVQQIKHGAINLLSGYRAAGHSFAG
                     IIATREGRNITGDWASQTDQLFYDPRLRPEQRTLTHSGLMDERWVFTNKDTGLEYKVS
                     AALAAASRSLRGFDDALASECLATAKKAWDYEQSHDPVIQPNAYVPRNTKVQEIIATS
                     ELLFTTGEEKYASHLKKLLPEIGKNMGRAAWCVARVADRLKDEEFNTAYLEMLREYKT
                     GLDSTLALNPFGVPWHPAIWGIGWNIQEFALEHYYLVTRYPDIFNREIVLRVVNYVLG
                     CHPGSSTSLVSGVGAHSVTNAFGVYRHLESYIPGGMVSGTALIRPDFPELKETTPFLW
                     QQSEYVMPGAASYIFCVLAADGLLNQNN"
     gene            complement(7400..9874)
                     /locus_tag="PK288_06760"
     CDS             complement(7400..9874)
                     /locus_tag="PK288_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPM18659.1"
                     /translation="MKKLIFLMAFCGLVLLSCSHDSIKRTWTVNEQEYLETQGFNVLV
                     FHNYYPEGDQGGIEFIHHDDRTATNGFIRVDLPQGQQFPRPVKAIRKVINDRNEIQAR
                     IEIPEYDFNYTVRVWPENDRIRLSVDLEKPIPSGWKGRATFDLQLFPVTYFGKTYMLG
                     DQSGVFPVEALGPMTSLPDGRIRPVPLATGKKLVMAPECQTCRLQIEQLNGDLSLIDE
                     RNTTLGGWIIVRSEIPENVDRGAVEWVITPSIIPVWLHKPVIGISQAGYHPAQQKKAV
                     IELDKRAGKPEMARLRKIENDGRMVKIKSGKPDLWGGFLRYKYVIFDFSEVKDPGLYT
                     VSYGNYESEPFRISRDVYRQSVWQPTLEIYFPVQMCHMRVRDRSRVWHGACHLDDARQ
                     APLSSILPDGYRTYSTTETRFSPDDHVPGLNRGGWHDAADNDLAAGSQAQTILFLSLA
                     GEAFGINVDQTTVSQDKLLVELRKPDGIPDLQQQVEHGVLNLLSGYRISGHSYVGIIE
                     TFEGRRFLGDVASVSDQLIYDPSLKPGEIRGNRSGKDDDRWVVTNKDTSLEYEVISAL
                     VAASRVLRGYNDPLAEECLAVAARAYNHEQTHEPVIQPNAYVPGNYEWQEVHATCELL
                     ITTGEKRYKDRLLELIPVIRKTPGRVGWSAARAMDFVGDENFKNELRQSLQNYRTTLD
                     STLAQNPFGVPWRPEIWGIGWNIQSYAVAQYYLCKAFPDLFDRENVCRVVNYVLGCHP
                     GSNTSLVSAVGAHSIIPAYGFNMHWWSYIPGGMVSGTALIRPDFPEMKEPFPYLWQQT
                     EYVMPGAASYIFCVLAADKLLNDSLL"
     gene            complement(9895..12168)
                     /locus_tag="PK288_06765"
     CDS             complement(9895..12168)
                     /locus_tag="PK288_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPM18660.1"
                     /translation="MKKVFCKSSFLLPAFIFLLFNPGSSSAQLIDPGVNVFVNQQPEE
                     EDLRVFRQWIKWNNPGSMLIHFITGETLKHYDVRSKEVAQLVTAEDWIGRQNSVRKKL
                     EDIIGDFPAKTPLNAKVTGVIRRDGYRIEKIIFESFPGFYVPGCLYIPEKIKGRIPAV
                     LHLMGHNQESFRNELYQVISYNLVKKGMVVFAIDPPGQGEHVQQYDPEIGFSSVGYSV
                     EEHNYLGNWCFLSGYSSAKYFIWDALRAIDYLVSRREVDSERIGVTGFSGGGTITSYI
                     GALDERVKVAVPCSWSNANRRLIETKGIQDAESHLYHGLMNGISNEDLIELRAPRPTL
                     LTFVSRDEYLCLQGAREAYTEALGAFRAFGRPDNLVLVEDDSKHWLTPKIRHAIYSFF
                     IKHFDLKVDPAEIEADILPREQLAVTPTGQIATFIEGKMIFDLNKEITADLLGRLENS
                     RKNDPDHIIKAIAGARDLSGFRLEGPSPGRLFINGKYQREGYSVGKYAIEVGDDYAIP
                     LLLFVPYGNNLKYPAVIYLHPEGKQADAGKGGRIEQLVRNGFVVAAVDLAGVGEVKNT
                     AGREPIDSYTALQIGRSIPGIRAENIVIAASVLSRLDIVDPAAIVGLGIGEMCIPLVH
                     AAAFSNVISSIVLDGPLVSYRTIAMNRNYRTGFTVRNGGDYWHPYEVDFSWGVGGALT
                     SYDLPDLIAGLAPRKVALNGIRNEMLQPADEEVIRQDLSFPFSVYSARGVPRNIMAGP
                     SGDTDYLSVLRWCLQKD"
     gene            complement(12619..13254)
                     /locus_tag="PK288_06770"
     CDS             complement(12619..13254)
                     /locus_tag="PK288_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPM18661.1"
                     /translation="MEIINQVVDWYMANLNYFTIAFLMTVESSFIPFPSEVVIPFAAY
                     KAAQGNLNVFGVVIAGTIGALAGALFNYYLAMYLGRPLVYKLAGSKAGRFLLLSEEKI
                     GHAESYFIKHGKSSTFIGRLVPAVRQLISIPAGLAKMNLKDFIVYTMAGAGIWNIILA
                     AIGYYIYDLRDKIFPYVDDILIAAGLIFVLYLSVKAIVQKKKQKKQITGSD"
     gene            complement(13517..15478)
                     /locus_tag="PK288_06775"
     CDS             complement(13517..15478)
                     /locus_tag="PK288_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HPM18662.1"
                     /translation="MKILSLKERPGGLDINRFSLAGLVITLGIVYGDLGTSPLYTMKA
                     IVGGGAGRIDQLLIYGSLSCVFWTLTLQTTVKYILITLRADNKGEGGVFALFALIRKK
                     SPRAAILTMIGGAALLADGVITPSITVTSSIEGLRMVVPEAPVVPVVLIIFALLFFFQ
                     QFGTNIVGGSFGPIMVIWFLMLGAAGTNQLIRHPEIFAAVNPVYAVRFLTEYPGGFIL
                     LGAVFLCTTGAEALYSDLGHCGRENIRISWIFVKAMLLLNYFGQGAWLMLNPQSLAGQ
                     NPFFSIFPGWFLIPGIIIATAAAIIASQAVITGSFTLISEAVSLNFWPKIRILFPAAL
                     KGQVYLPFVNWFLWIMCSIVVIAFRESSNMEAAYGLAITIAMIMTTMLLSYYLYQRKT
                     SRRFVFPVLIVFLAIEGTFLIANLHKFWHGGWLTLLLATIYFLVMCGWYFGRKIKNRY
                     ITFSRLTDYLPMITDLVRDQTIPRTATNLVYLIKANHKDQVESKVMYSIFYKQPKKAE
                     TYWLLHVDRVDDPYRFDYEVEHILPGILVRIDLHLGFKVEPRINLYFKEVLEDLVSTG
                     EIALFSSFSSLKKHNFPGDFVFILIERIMIRDFKLSRWENIILALNAVASRLSISEIK
                     ALNLDPTNSMVEQVPIIIDRKIPVRIKRI"
BASE COUNT         4261 a         3715 c         3356 g         4165 t
ORIGIN      
        1 tcgctcaagg ctatgtcaat caccgccagg tcattgagct tcaccagagt gtcaacggga
       61 tcataaccgg tgaagcttac ccgaagattg tagtatccgt cccgaaggtt gcggagagcg
      121 aacctgccgt cattgtcagc agccacaccc gtaccggttt cgactatgat cacagaagcc
      181 cctgccagcg gcatacctcc gttaccagtg atccggcctg tgatcgtact ggcagaacgc
      241 tggcctgata tcatcccggc cagagtcagc agtgcaaaca ttgaaactaa aatctttttc
      301 atcctcagtc ctgatatcca aagtttaaac acaactgtgt cagaagagaa gatgtcagaa
      361 caagccggaa atgattactc actttcccta cgccggtatt acccggatca ggttttaagg
      421 gtatgatctc agcccgtaat tttaaggcac cccctctttg gcaatgcaaa tttacaaatt
      481 ttatctggta ttatttaaca aaagctgttt tttacatgtt gccttttttg taaattagcc
      541 ttctaatcac ctgaatcgta tgaaaacact cctggataag ctaaattaca gagggcaatt
      601 gcgtattgcg attatcaatc ccgaaagcaa tttcaaactg gcaccatcca ggatccttac
      661 aagcatacag atagatgatg tgatagacca gaggtttcct tatggcttta tgatgatttt
      721 cgtaagggaa ccgaaggata ttgagaaata tgctccactg gccctgcata atcttgcaac
      781 tgacgggctt ttatggttct gttatcccaa aaaaacatca aataaatact gcccgggaat
      841 agacaggaac cacggatgga aagttctcaa tgaaatggat ttttacggga cgcgggttgt
      901 taccatagac gacgactggg ccgcgttgag attcaggaac aagaagttca tcaaatccag
      961 ggctgacaag tcgtccaagt agtaacaacg ggctctcacc tgtattggaa gctgaaacga
     1021 ttaataatcc tgctccccgg acttagcatt tcagagcagg aacattattc cggaaatgat
     1081 caaaattgcc actatagaaa ctccgattgg tgaaatgacc gttgctgcca taaaagaagg
     1141 catatgcatg cttgagttca cggaggccag gaaacccgga gcagattatg caaagcttag
     1201 gcagatcttc aacatggatg cagattttgg aactaacagg cataccagag ctttgaaaaa
     1261 acagctgaaa gaatatttca gcggaaaaag aaaagagttt tccgtcccgc tcttctatca
     1321 tggttctgat tttcagaaat cagtctggag gatattggaa aagatcccat acggtaaaac
     1381 catctcatat atgcagctgg ctgagatgct gaacaatccg aaagcaatca gggcagcagc
     1441 aaacgcaacc ggatcgaatc ccctcgcaat aattatacca tgccacaggg ttgtaggctc
     1501 tgacggagag cttaccggat acaggggtgg tatcgaaagg aaaagctggc tcattgatca
     1561 cgaaagaaga ttctctggac agcctgtgga tggcatcctt ttttaactat cacgaaaaat
     1621 ttcagaaatg aattcatcat taaaaaatca ttattcattt ttattcctgt cgctattatt
     1681 atttatcata ctcgccggct gtgcacgcag tgaacctgta gatcattgcc ttacagggca
     1741 cacgtacggg ttcttcggag gtttgtggca cgggttcatt gccccttttg acctgattgg
     1801 catgctgttt aacaagaaaa tcaccatgta cgcccaaaac aacaatggcg gattttatgc
     1861 gcttggattt ctcctgggaa gcggaggttg gggatttttt ggcagcaggg gatcgcgccg
     1921 gatctatcac cggaagataa gcgtaaaaag cgacagattt gatgaggcac aaatagtaga
     1981 ataaataagc agatcgtctg acccttcttc cggaattaat aacaggattt tcatgaaatg
     2041 tcatgatgag acacgtcagt cataggtatg gataatattg agaaaatcat caatatcagg
     2101 agtgctttca gaaacagcaa ttcggtattt ctccggtcaa ttcccggctt ttttgtaaca
     2161 atacttgaaa agctcatttt ccagcacgaa atgaatgcaa ccattcacag gagccgtgac
     2221 aaggaaggag tcccgttcat aaatgacgta ctggaaggat ggaacgtaaa ggttaaaatt
     2281 atcggcgggg aaaatgttcc tgctgccggc aggttcattt ttgccggtaa tcatcctgtg
     2341 ggaggcattg atgcactggc tttctatagt acgatataca aatttttccc tgatgtcata
     2401 tccccgacaa acgagcttct gaaccagata cccaatctga ggccggtaat gctgggcatt
     2461 aatgtttttg ggaaaaatac cagggaaaat gctgcaagga tagatgaact ttttgaatcc
     2521 gattcacaga taatgatttt tccctcaggt gaggtttccc gtaaaataaa aggagtaata
     2581 tccgacccgg tatggcagaa aacatttgtg acaaaggcaa tccggcataa aagggatata
     2641 ataccggttc atatcagcgg caggaattcg ggattttttt attttgttgc cagcctgcga
     2701 aaattccttg gtgtcaggat gtatctggaa acaattcttt tgcccaggga aatgatgaaa
     2761 caaagaaatt ccgaggttac aatcacaatt ggaaaacctg taagccacct gtctctgaca
     2821 aaagataaaa cccattatga atgggcccag gagatcagaa aagccgttta caacctgaat
     2881 ataagttaaa caatacggtt gtttgcctgt tgtacctgta aggaaaatta ccagatcatg
     2941 acgaatatca ggaatattga ggctataatt ccgcctccgc cataccatat ggtgggcgat
     3001 ggattcaaag ttcataattt cttcccatcc agccgtatta tcggggatac agggatgagt
     3061 ccattttttt taatggatta taatgcgaag tgggaaataa ccccatccga aagacccagg
     3121 ggagtcgggg ttcatccgca ccgcggaatt gaaacaataa ccattgcata tcacggcagg
     3181 atcgcccatc acgacagcgc cggcaacagt ggtgttatcg gtgagggaga cgttcagtgg
     3241 atgacagctg gaggaggggt gcttcacaag gaatatcatg aaaaggagtt cagccggaaa
     3301 ggaggtcttt tccagatggt ccagctatgg ataagtcttc cgaaaagatt caagatgacc
     3361 ccgcctaagt accagacaat agaaagcaag gatatgacac gtgtgatgct gaaggacggt
     3421 gtaagccatg tggatctcat tgctggcagt tacatggaaa agaaggggcc ggcatctacc
     3481 ttcactccta caaatctttt caacgccagt ctgaaaaagg gtatcagggc tgatttcagc
     3541 tttcccttgg aatacaatac cggcctgctc gtaatcgagg gtgagataaa tatcaacgga
     3601 tcgcggaatg ccacggctga taactttgtg tatttcagcc atgacggaga aaaatttact
     3661 gttgaagcag taaccgacag tattgttctt ttactgagcg gtgaaccggt caatgaggaa
     3721 atttatccct atggcccttt tgtaatgaat accagggatg aaataaaaca ggcatacgaa
     3781 gatttctata acggcaagtt cggccatctt gaagactgat gaaagattaa tggccaaccc
     3841 ctttataatg aatcattttt cagtaattcc cctgctgctg aagcaggtcc ttttcttttc
     3901 ttacccggta aaataaggca gtcttgttac ttttggatca aaatcgagat ttttgacaat
     3961 ttcttctgct tcccgtgctg aaataattgg tcgtttccca atttccacac acgattccaa
     4021 tggtgcaaac ccgtccattc cgacattggc aacagttacc ggaagtgaca gtgcaaatct
     4081 caaaaggtcg ttccccgaaa catttccgat gagggcattc tgtcccatta ttttgattgc
     4141 aatgatgccc aggttccttt caatggccaa tggaatcagg ttatcctcat ttccgttcga
     4201 cctggcagca ttaatcacac acattaccgc atcgatatca aattccctga acgatttttg
     4261 cgatgcctcg ttccatttgt gaaatgaaaa tccgatgttt ttcaccacac cgtcattcct
     4321 tagcttgaga aatgccttat atccacctga ctgaccaagc aatgtattta catcttccat
     4381 tgtatcaatt gcatgcatac agtacagatc gagataatcc gtcttgagaa gggaaagtga
     4441 tttttcaata tcccgcataa cgccgtcata ggttctctcc ccggattttg agttgagaaa
     4501 tatttgttcc ctgtattttg gggtaaggaa ctttccgtag cgttcctcgc acaatccgtc
     4561 gccatatgac cttgcagaat caaaataagt aatgcctagt tttatggcat gatcaatatg
     4621 cttctgaagt atttcatccg gggcgatcca gttggtatat cttgaaccgc ctccgaatcc
     4681 aatgcatgac accatcctgt tggttcttcc aagcctgcgt tttggcaaac cctccgcttt
     4741 aactgctgat tcactggtgg cagcaactgc agctgaactg cccaaaacag gtatcgtacc
     4801 aactgcagca gtaactgctg caacccggat aaatctgcgt cgtggaattt tcgttttcat
     4861 ttaatccagt tttttgtcaa agatataaat tgattggatc agttgttctg gttcaacagc
     4921 ccgtctgctg ccagtacgca aaagatatag gaagctgccc ccggcattac atattcggat
     4981 tgctgccaca gaaaaggtgt tgtttctttc aattcgggaa aatcgggtct gattagtgcc
     5041 gttcctgata ccatgccccc gggaatatag gattccagat gcctgtaaac cccgaaagcg
     5101 ttggtcactg aatgtgcgcc tacacctgaa accagggatg tactggatcc cggatgacag
     5161 cccaggacat aattgaccac cctcaacaca atttcccggt taaaaatatc aggatacctg
     5221 gtcacaagat agtagtgttc aagtgcgaat tcctggatat tccatccaat gccccagatt
     5281 gcgggatgcc atggtacacc gaaaggattc agtgccagtg tggaatcgag ccctgttttg
     5341 tattccctga gcatctcaag gtaggcggta ttgaactcct catcttttaa ccggtcagca
     5401 actctggcca cgcaccatgc tgccctaccc atatttttcc ctatttcggg gagcagtttt
     5461 ttcaagtggg atgcatattt ttcttctccc gtggtgaata aaagctcaga agtggctatg
     5521 atctcctgaa ctttggtatt tcgcgggaca taggcgttag gctggatgac aggatcgtgt
     5581 gattgttcat agtcccaggc tttttttgcg gtggcaaggc attcagaagc cagggcatca
     5641 tcaaatcccc tgagtgaacg gctggcagca gccagagccg ctgaaacctt gtactcaagc
     5701 cctgtatcct tgttggtaaa tacccatcgt tcatccataa gcccggagtg agtcagggtt
     5761 ctttgttcgg gccttaggcg gggatcatag aacaactgat ctgtctgcga agcccagtcg
     5821 ccagtaatgt ttcttccttc acgggtggca atgattccgg caaagctgtg acccgctgcc
     5881 ctgtagcctg aaagaagatt gatagctcca tgcttgatct gctggacaaa atcagggatt
     5941 ccgtcgggtt tatgcatttc aacgtacaga ccctggaaat cgacggatgt ctgatcagtc
     6001 tgattattgc atatctcatt cgccagtacc agataatgag ttgttgcagc ctgtgaacct
     6061 gcggcaagat catcatcgcc ggcgtcgtgc catcctcccc tgttctgaaa tggaaccgtt
     6121 gtaaggggtc tgtaactggt ctctgcctcc ttgtactgcc tgtagccatc aacgtgctca
     6181 atgtcgaggg gagcctggag tgcatcatca agatgacagg caccgtgcca gattgcctgt
     6241 ccgtccctga cctttacatg acacatctga accggaaaat atccctcaag ggtaggttgc
     6301 cagactccat ttctgtaaac atcctgatct attgaaaaag gattggtgga ttttgtcccg
     6361 taagatacaa aatacattcc tggttcctta acagctgaaa aatcaaaaac cgcatacttg
     6421 tagcacagga aatcgcccca gggtttaggc gatcctgaaa gtacttccct gaccttacct
     6481 cccgaaactt tcatcagacg ggccttttcc ggctttccgg ttttccggtc gagttcaatg
     6541 accgcctgct tgacctgatc aggatgatag ccaacctgtg aaacggcaat cacaggctcc
     6601 ctgatccagc cattaacaac attcggcctt atgatccatt ccaaagctcc ttgggtcaca
     6661 cctggggata tgaccgatct gaccatgatc cagccgcttt tgcttgattc acggttatct
     6721 gtaagaataa tttccccttt cttattttca aagacgattt ttcttagagg atcttctcct
     6781 gccagtgtca gtatttttcc ctgccccata tttacgggac taatccttcc gtcttcatcc
     6841 gtaaaaacgg atctcacacc catcctgggt attattccaa aagtctcacc tatctggaaa
     6901 gttttgccat aataaagcgg cggataaact tccatttcga aactgagcct gttctcccat
     6961 tccgcaggca cgggtttctc aaggtctaca attaatttaa agccgttgcc ttcaggttta
     7021 agcctcactt tataattcag gtcaaaatcg tcatagctta tggaagagac gatttcctgt
     7081 ttctgcctgt ctgcttcacg ctctgccctg tcagggcctg gcagcctgcg ttcatctgcc
     7141 ggacgaagcc tgataaatcc attggttgcg atccgctctc catgctggat tatctcaatt
     7201 cctccctggt ggcccacggt gtaatagtta tggaatgcaa gtaccgataa acccggtttt
     7261 tcaagatatt cctgatcgtt gataatcagt cggtcgcttc ctttatcccc ggaacaacct
     7321 gtcagtaatc ccaaaaagga tacgatgaca aatagtttaa acagatcggt ttttttcatt
     7381 gtgatataag tgaaagtatt tacaataaac tgtcatttaa taacttatca gctgcaagca
     7441 cacaaaagat ataggaagct gcacccggca tcacatattc agtctgctgc cagagatacg
     7501 gaaacggttc cttcatttca ggaaagtcgg gcctgataag agctgtaccc gaaaccatac
     7561 cccccggaat atatgaccac cagtgcatat taaacccgta tgcaggtatt attgaatgtg
     7621 cacccacagc tgatacgagg gaggtatttg atccaggatg gcagccaaga acataattta
     7681 ccaccctgca gacattctcc ctgtcaaaca gatcaggaaa ggctttgcag agataatatt
     7741 gtgccacggc ataagattga atattccatc ctattcccca tatttcgggt ctccagggta
     7801 ctccaaacgg attctgtgcc agggtggaat cgagtgtcgt cctgtaattc tgcaacgact
     7861 gcctgagttc atttttaaag ttctcatccc cgacaaaatc cattgctctt gcagcagacc
     7921 atccaacccg gccgggggtt ttcctgatga ccgggatcaa ttccagaagc ctgtccttgt
     7981 aacgcttttc cccggttgtt atcagcaatt cacaggttgc atgaacctcc tgccattcat
     8041 aatttcccgg aacgtaagca ttaggctgaa taacaggttc atgtgtctgt tcatggttat
     8101 atgcccgagc tgccactgca aggcattctt cagccagcgg atcgttatat ccgcggagta
     8161 cgcggctggc agcaacgagt gctgaaataa cctcatactc cagggatgta tccttgtttg
     8221 tcaccaccca ccggtcatcg tctttccccg accggtttcc ccttatctca ccgggtttca
     8281 gggaaggatc atagatcagc tggtcagaaa cggaagcaac atctccaagg aacctccttc
     8341 cttcaaaagt ttcgatgata cccacgtacg aatggcctga tatcctgtat cctgacaaca
     8401 gattcaatac tccgtgttcg acctgttgct gaagatcagg gattccgtca ggttttctaa
     8461 gttccaccag gagcttatcc tgagaaacgg tggtctgatc tacattaata ccgaatgcct
     8521 cgccggccag tgagagaaac aggatagtct gggcctgtga tcctgcagca agatcattgt
     8581 cggcagcatc gtgccatccc ccgcggttca aacccgggac atgatcatcc ggactgaacc
     8641 tggtttctgt tgtcgagtat gtcctgtatc cgtcggggag gatggatgac aatggtgcct
     8701 ggcgtgcatc atcgagatga catgctccat gccatacacg tgatctgtcc ctgacacgca
     8761 tatgacacat ctgaacgggg aaataaatct caagggtcgg ctgccagact gattgccggt
     8821 aaacatccct gcttatcctg aaaggttcgc tctcataatt accataactt actgtgtaga
     8881 gcccgggatc ttttacttcc gagaaatcaa aaatcacata tttgtacctc aggaatccgc
     8941 cccataaatc cggcttgcct gacttaattt taaccatcct gccgtcattc tctattttcc
     9001 tgaggcgtgc catttccggt tttcccgccc ttttatcaag ttcaatgact gcctttttct
     9061 gctgagcggg atgatagccg gcctgtgaaa tgccaataac cggtttatga agccataccg
     9121 ggataatgga tggagtgatc acccattcaa ctgctcccct gtcaacattt tccggaatct
     9181 ccgatcttac gattatccat cccccgaggg ttgtgttcct ctcatcgata agcgacaaat
     9241 ctccgttcag ctgttcgatc tgcagcctgc aggtctggca ttcaggagcc atgaccagct
     9301 ttttcccggt tgccagaggc acaggtctga tccttccgtc cggtaatgat gtcatcggtc
     9361 ccaaggcctc gacaggaaaa acgcccgact gatcccccag catataggtc tttccgaaat
     9421 aagtaaccgg gaaaagctgg agatcaaagg ttgcacgtcc tttccaaccg gaaggaatgg
     9481 gcttctccag atcaaccgat agtcttatcc tgtcattctc aggccagacc cggacagtat
     9541 aattaaaatc atactcagga atttcgatac gtgcctgtat ttcgttcctg tcatttatca
     9601 ctttcctgat tgccttgacc ggtctgggaa attgctgtcc ctgcggcaga tcaaccctga
     9661 tgaaaccgtt tgtggcagtc ctgtcatcat gatgtataaa ttcaatccct ccctgatcac
     9721 cctcagggta ataattatga aatacaagta cattgaaacc ctgtgtttca agatactcct
     9781 gttcatttac cgtccaggtc ctctttatac tgtcatggga acacgacagg agaacaagtc
     9841 cgcaaaatgc catcaaaaaa atcaatttct tcataatatc agtttttatt tgtttcaatc
     9901 tttctgaaga caccaccgga gcactgaaag ataatccgta tctccggatg gacccgccat
     9961 gatattcctt ggaacacccc tcgcagaata cactgaaaac gggaaggaga gatcctgcct
    10021 gatcacctct tcatcagcag gctgaagcat ctcattcctg atcccgttaa gtgccacttt
    10081 tctcggtgca aggccggcaa tcaggtcggg aagatcgtaa gaggtaaggg caccgcctac
    10141 accccatgaa aaatccactt cataaggatg ccagtaatcc cctccatttc tgacggtaaa
    10201 gcctgttctg taattcctgt tcatggctat tgtcctgtaa gaaacaagtg gcccatccag
    10261 aacaatgctg cttatcacgt tactgaatgc agcagcatga acaaggggaa tgcacatttc
    10321 accgatcccc aggccaacaa tcgcggcagg atcgacgata tcaaggcggc tcaggacact
    10381 ggctgcaata acaatatttt ctgcacgtat ccccggaatg cttctgccaa tctgcaaggc
    10441 agtatagctg tcgattggtt cccgccccgc agtattcttt acttccccaa caccggcaag
    10501 gtcaactgct gcaacaacga atccatttct taccagctgc tctatacgcc cccctttccc
    10561 cgcatctgcc tgtttaccct ccggatgaag ataaattacg gcgggatatt tcagattatt
    10621 tccataggga acgaacaata gcaagggtat tgcataatca tctcctactt ctatggcata
    10681 tttcccaacg ctatatcctt ccctctggta ttttccgttt ataaaaagcc tgcccgggga
    10741 agggccctca agcctgaagc cggataaatc cctggccccg gcaatggcct tgattatatg
    10801 atccggatca ttttttctgg aattttcaag tctgcccagc aggtcagcag taatttcctt
    10861 atttagatca aaaatcattt tgccttcgat aaatgtggca atctgaccgg tcggtgtgac
    10921 tgcaagctgt tcgcggggta atatatccgc ctcaatttca gccggatcaa ctttgagatc
    10981 aaagtgtttt ataaagaaag agtaaatggc atgcctgatc ttcggggtga gccagtgctt
    11041 tgagtcgtct tcaaccagga ccagattgtc aggcctgcca aaagcacgga atgcacccaa
    11101 agcttcagta taggcttccc tggcaccctg cagacagaga tattcatcgc gtgagacgaa
    11161 tgtaagcaag gttggccggg gagccctcag ttcaattaaa tcttcattgc tgatgccatt
    11221 catgagccca tggtacagat gtgattcggc atcctgaatt cccttagttt caatgagcct
    11281 tctattggca tttgaccagc tgcagggaac agcaaccttc acccgttcgt caagtgctcc
    11341 gatatatgaa gttatcgtgc ctcccccgga aaaacccgtg acacctatcc tttcactgtc
    11401 cacttccctg cgcgacacca gatagtcaat agcccttaaa gcatcccaga tgaagtattt
    11461 cgcacttgaa tatcccgaaa gaaaacacca gttccctaga taattatgct cctccacgga
    11521 atagcctact gaagaaaatc caatttccgg atcatattgc tgaacatgtt cgccctgccc
    11581 cggaggatca attgcaaaaa ccaccattcc cttctttacc agattataac ttatcacctg
    11641 gtaaagttca ttccggaacg attcctggtt gtggcccatc agatgcaata cggccggaat
    11701 tctgcccttg attttttcgg ggatgtaaag gcatccgggc acataaaagc cggggaacga
    11761 ttcaaaaatg atcttttcaa tcctgtatcc atcccttctg atgacacctg taactttcgc
    11821 attcagcgga gtcttcgccg ggaaatctcc aattatatct tcaagtttct tccttacact
    11881 gttctgccgg ccaatccagt cttcggccgt aaccagctga gccacttcct tactgcggac
    11941 atcataatgt tttaaggttt ccccggtaat aaaatgtatg agcatgctgc ccgggttatt
    12001 ccatttaatc cactgccgga acaccctgag gtcttcttct tcgggttgct ggttaacaaa
    12061 aacattgact ccgggatcga tcaactgtgc gctggatgat ccaggattga ataacaggaa
    12121 aataaaggct ggaaggagaa aagagctttt gcagaacacc tttttcataa caatcagatt
    12181 accaggcaga ttactctagt tcaatgaatt acttcattaa atatcaataa atttaagaat
    12241 aaaatcaaca tgcaggggcc tttataattc ctaaatcatg gcaggattat catttgatac
    12301 agggctttta ctcgccaatt cccggcgacg gacataagag ggactgcctt tatgcttatt
    12361 tccggaaagt tttcttgttt ttaatacgga tttgaattaa ctccatacac aaatgaatca
    12421 tctgttccgc agcagacggt caaaattcac ttgcaagctc ctcaggaaaa ggggaggtct
    12481 gttctgttaa ttccttaaca ataccgcaat acgttcatca acactgctta ctgcaccaga
    12541 ataattgcaa gaaccaaaac tccgcagggc tgagttaatt taaaggtttg ttgaaggaga
    12601 ccttttctca acaggatact aatcactgcc agtgatctgc tttttttgct tctttttctg
    12661 aacaatggct tttacagaca ggtataacac aaatatcagg cctgcggcta tcaggatatc
    12721 atccacatag ggaaagattt tatccctcag gtcataaata tagtatccga tcgctgccag
    12781 gataatgttc catatcccgg ctccggccat ggtataaaca atgaagtcct tcaggttcat
    12841 tttcgcgagc ccggccggta tggaaatcag ttgtctcaca gcagggacca ggcgcccgat
    12901 aaatgttgat gattttccgt gtttgataaa gtaggattcg gcatgtccga tcttttcctc
    12961 cgacaaaaga agaaaccttc cggcctttga cccggctagt ttataaacca gcggacgtcc
    13021 cagatacatt gccaggtaat aattgaacaa cgcacccgca agagcaccaa ttgttccggc
    13081 aataactacc ccgaaaacat taagatttcc ctgggcagcc ttgtaggcag caaaaggaat
    13141 tacaacttct gaggggaaag gaataaatga actttccacc gtcatgagaa aggcaattgt
    13201 gaaataatta agattggcca tgtaccaatc gacaacctgg ttgattattt ccattgtaaa
    13261 taactggtaa gacttataaa cacattaatg gttcctttta ttatactcat catcatccac
    13321 ggggaaacaa agttgcaaaa ataacagaat catgcaattc agtggtagtt attgaatcag
    13381 caacagtcag gcacccgttg tttttatgcg gcaataatac ctgaattatc ggtaccggta
    13441 ctgcatatga ctgcagttca gaccgaaaaa ccaacatgga tttaaatatt cggtcaggat
    13501 atggcatata aaatgatcag atccgtttta tcctgaccgg gattttccgg tcgataataa
    13561 ttggcacctg ctcaaccatt gaatttgtcg gatccaggtt cagtgcctta atttccgaga
    13621 tactcaaccg gctggcaacg gcgttgagag caagtattat gttttcccat ctcgagagtt
    13681 tgaaatcacg gatcataatt ctctcaataa gaataaaaac aaagtcaccc gggaagttat
    13741 gctttttaag ggaactgaaa ctgctgaaaa gagcgatttc tcccgtgctt accaggtcct
    13801 caagcacttc tttgaaataa aggttaattc tcggttcaac tttaaaacca aggtgcaaat
    13861 caattcttac caggatacct ggcagaatgt gctcgacttc ataatcaaat cggtaaggat
    13921 catcaaccct gtcgacgtgg agaagccagt aggtttcagc ttttttgggc tgtttataaa
    13981 aaatcgaata cattaccttc gattctacct ggtccttatg atttgccttg atcaggtaaa
    14041 ccaggtttgt agccgtacgg ggaatggtct gatcccttac caggtcagtt atcattggaa
    14101 ggtagtccgt aaggcgtgaa aaagtgatat acctgttttt tatttttcgt ccgaagtacc
    14161 agccgcacat taccaggaaa tagatcgtcg caagcagcag tgtgagccag cctccatgcc
    14221 aaaacttgtg aaggttggca atcaggaagg ttccttctat tgcaaggaat acaatgagca
    14281 cgggaaaaac aaatcgtcgt gaagtcttcc tttgataaag ataatatgac agtaacattg
    14341 tggtcatgat catggcaatg gtgattgcca aaccgtaggc agcctccatg tttgatgatt
    14401 ccctgaatgc aatgactaca atgctgcaca tgatccacag gaaccagttc acaaaaggaa
    14461 gataaacctg acccttcagg gcagcaggga acaagatcct gatctttggc cagaagttta
    14521 gtgagactgc ttcgctgata agtgtaaatg agcctgttat taccgcctgg ctcgctatta
    14581 ttgcggcagc cgttgcaatg attatccctg ggatcagaaa ccacccgggg aaaatggaaa
    14641 agaaaggatt ctgaccggca agtgactgag ggttaagcat tagccaggcg ccctgcccaa
    14701 aatagttaag gagaagcatg gcttttacga agatccatga aatccttatg ttctcccgtc
    14761 cgcaatgacc cagatcggaa tataaagctt cggcgcctgt tgtacaaaga aagactgctc
    14821 ccaggagaat aaatccgccg ggatattcag ttagaaatct gactgcataa acaggattga
    14881 cggcagcaaa tatttccgga tgccggataa gctgattggt tccggctgcg ccgagcatca
    14941 ggaaccagat caccattatc gggccaaatg atccgcccac tatgtttgtt ccgaattgct
    15001 gaaagaaaaa cagtagggca aaaattatca ggaccacagg aaccaccggt gcttccggaa
    15061 caaccattct taatccttcg attgaagatg tcactgtgat cgatggagtg ataaccccgt
    15121 cagccagcaa tgctgccccg cctatcattg tcagtattgc cgcccgcggt gatttctttc
    15181 ttatcagtgc aaagagggca aatacacctc cttcaccctt gttatcagcc cttagggtta
    15241 ttaatatata cttgacagtt gtctgcaggg tcagggtcca gaaaacgcaa gagagcgaac
    15301 catatatcag gagctggtca attctcccgg caccaccccc aacaattgcc ttcatggtgt
    15361 agagagggct tgtgcccagg tcgccataaa cgatacccag cgtaatgacc agtcctgcca
    15421 gggagaacct gttgatatcc aatccgcctg gcctttcctt taggcttaaa atcttcatat
    15481 ttccttcatg cgttgta
//
LOCUS       DAOOYG010000061        15380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_3833_length_15380_cov_8.338010, whole genome shotgun sequence.
ACCESSION   DAOOYG010000061 DAOOYG010000000
VERSION     DAOOYG010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15380
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3833_length_15380_cov_8.338010"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PK288_06780"
     CDS             complement(<1..907)
                     /locus_tag="PK288_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18663.1"
                     /translation="MKTHCLPLVFLASVIVSFACSRPPEPQEPVLLRENWAIQSSAAV
                     GESGSVISTTAFKPENWYPATVPSTVLGTLVENKVYPDPYFGTNILKIPGYITKWPPI
                     VPDDSPFKVSWWYRTEFHLPAGNGAKNTWIRFHSINYKANIWLNGSLIADTASVQGAY
                     RIYDLNITDYAKPGEINCLALEIFPPERFDLTIAWVDWNPTPPGRGMGIWYDVTVRST
                     GPVTVKNPQVITKLNLPSTDQARLTIASEVENLTDGKIKGKLKGTIDNIVFTQEVTLG
                     PKESKKVVFDPSDFSQLVISEPRLWW"
     gene            complement(924..3113)
                     /locus_tag="PK288_06785"
     CDS             complement(924..3113)
                     /locus_tag="PK288_06785"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HPM18664.1"
                     /translation="MRYRILKKRYLITACIFLSVALLLLAVPGIARWYIEKNSMELIG
                     RRITIDRIGIRYLTGTLKVRGFKIFEADEKTVFASFRQFRISVSYLPLLRNEFVIRKV
                     SLDDPFIQVMQNGSMFNFSDLVAPDSTENERENVRKTPAKYVIHNIRIAGGFVKYTDV
                     PLQHTIYLDSIDLTIPGFTWNSDSARLGAGFRFVEGGGISSDLEINQADSTYRLSLMI
                     DSLNLGITEPYVRNYLNVSTLTGNLTSDLMINGSISSILNLSVQGTSQVSNFGLQDTL
                     GRTILSFNDLSVKIDTLDLSAGRLHLGSISLTDPFVLMELQDSVTNWTTLMKTGESAD
                     SAAISADTTGITGGFRYNIPEVSVSGGSILYMDKNLRYPFEYKIDSLKLNVKEITDQP
                     GRISYDVAAGLNGTGKLITHGMFSLENPGSDVELSLDISRLRMKDLDAYFRHYLGFPV
                     TGGTMNLRTDNKLKGKSLVSNNEIYFRRFSLGKKVADETRFNVPLRLALAVLSDRNGI
                     IDLKAPVRMKGEEVRIVNLGKIIFRIIGNLFVKAAVSPINLLSGLIQSDPSSLQSVRL
                     NLTEFQPDEKAIRSLDAIAGILDKKPMLKADFIYCINRGRAADTLAGIMARKELMNSG
                     ILREKDIRNIADSSLFRYLRNKLTLPDGTDSLPLAALSRQYFGEDVLSSSLDSLRAGH
                     AGYLKKYLATERNIPAERFSVIEITPDSLVPVKGDPLFRIYFTAEDQ"
     gene            3449..4801
                     /locus_tag="PK288_06790"
     CDS             3449..4801
                     /locus_tag="PK288_06790"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM18665.1"
                     /translation="MKTRKFTRRQVIAATSAGFLGSMLNYSFRPFTVAGTSQSVLAIN
                     GGEKVHKGPWSDWPFWDRSAEPGILEMFRSGRWWRGEGEHVAEFEKKYAELMGARSCL
                     ATASGTTSLIIAMEALGVDAGDEVLISPYTFIATYNSVFAHKALPVFVDSDPATFLMD
                     PAKIEEKITDRTVAIIPVHIYGLPVDMDSVNSVARKHGLKVIEDACQAWLAEYRGKKT
                     GTLGDLGCFSFQNSKHLPAGEGGAILSMDEALVDKCYAIHNCGRPRGTMKPGPGYPYR
                     GRNYRMQQSQALILLSQMKRIEKDNDVRLSNALYLDRKLREIPGIIPYRPANGATRAV
                     YHLYPFRYIKEEFNNIPKEKFIEALNAEGIPATGGYGKQNKDGLMEEALTSRGYKRLF
                     SEARLRQWREQNNLPGNDQLCDQAVTLYQSILLGSKADMDDIVNAITKIHKLKNTLRP
                     "
     gene            4926..6320
                     /locus_tag="PK288_06795"
     CDS             4926..6320
                     /locus_tag="PK288_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPM18666.1"
                     /translation="MGRLLEEGEFAELEKLADSLKTLRLSDNDLVAKADSFVQIAGRI
                     AIDFSVPEADFIDDLKKRTGGYSLEEFRLWEKNNWLERRKINGEIRYFRRAASNLVRL
                     RDFNLRRAYFDSLNSVSPEIRFRLKHIESVIRAARENSAPVVPVEMTITYSLTVKSDA
                     VPEGEIVRCWLPYPREDQPRQQKVKLISASHGNYIVSPDSVTHRTIFMEGRAVKGIPL
                     VFKVEFSYQFGAQYYDPDNINNLPSSGYSGICDKYISEQLPHICFTERVKKLADSIAG
                     NEKEPFRILKKLYYWIDSNIPWAGALEYSTIPCIPEYVLANRRGDCGMKTFLLMSMLR
                     YKGIPVRWQSGWMMPPDDENLHDWCEVFIEGTGWLPVDMSYGLQYSDDKKIKEFYLSG
                     IDSYRLIVNNGVSGKLFPPKKFLRSETLDFQRGEVEWEGGNLYFNEWTYNMGIEYTAK
                     SNYSEKRNRMQLTP"
     gene            6335..7531
                     /locus_tag="PK288_06800"
     CDS             6335..7531
                     /locus_tag="PK288_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPM18667.1"
                     /translation="MKQTRLPCFLLLLAGGLLSGISLPGCRQDSLVLLSEGLDSIGAS
                     VVKDKREGIFNINLYRAGEKIILSGETDSRDARNSVLGYLEGKGVRFIDSLMLLPDTA
                     ALGTQWGLVNVSVCNMRLVPSYDAELISQSLMGTPVRLLKRDHGWFLVQTPDRYIGWV
                     DSDAIAVLTSEEYRDWKSSERIFFTGKTGDIYGDPEKKTTVSDIVAGCILKVSGISGR
                     DYRVILPDGRSGFIPASGAMILDDLDPVRLLTPETLISRAESFNGIPYLWGGTSVKGF
                     DCSGFVKTVYYLSGIILARDASLQFRHGTETDTVNFPASLKPGDLLFFGSVREGRPRA
                     THVAMYTGNTEYIHASGMVRTNSLDRSRSNFSGYRRDTFIGAKRIIGAGYGEGIIPVA
                     GHPWYR"
     gene            7535..8677
                     /locus_tag="PK288_06805"
     CDS             7535..8677
                     /locus_tag="PK288_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPM18668.1"
                     /translation="MNKRTFIKYCTSLAGISLVGRNFAGAATTFPVNRYNMKKSGTLK
                     LRYAPYTLQLKHAFTLATSSRTTTPVVLTELEFEGITGYGEASMPPYLGESHESVLKF
                     LAEVDLSRFSDPFLLEDILIYVDNVMPGNYAAKASIDIALHDLIGKITGQPCYRLFGL
                     NPAKTPLTSFTIGLDSPEIIKQKVEEAEPYRILKVKLGRDNDREMIETIRSLTDKPLC
                     VDVNQGWTDRNHSLEMAHWLHEKGVIFLEQPMPRSRKDDIAWLTQNSPLPIIGDEAIQ
                     HADDIVSQKDVYSGVNIKLMKCGGMHAARNMIGLARALGLKVMIGCMTETSCAVSAAA
                     QLSPLVDWCDLDGNLLISNDVFDGMKITGGKVTLPDRPGIGVIKLT"
     gene            complement(8707..10020)
                     /locus_tag="PK288_06810"
     CDS             complement(8707..10020)
                     /locus_tag="PK288_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18669.1"
                     /translation="MKSNRRGFLKAAGTLGIGLAGFDFPEKINSDYRHNKSNDIYMNS
                     ETESTNMERPITVQDQVSLIGVYGSWAAGLSGKNLPRLSFRRKEWKDVKSWKTVAKER
                     LFERVVMPETGKSPEVQQLKSYTFDGLHIEELRWQLPYGRPTDAIFLKPAGAVGKLPG
                     ILAFHDHGGNKYFGTRKITKTSADQHPLMKDHQEHYYEGRAWANEIARRGYAVLVSDA
                     FPFASRRVMMQDVPVHLKQGLTDDDPEDPANVNAYNNWAREHEHIMAKSLFSAGTTWP
                     GVFMAEDSVALDILCSRKDVDSSRIGCAGLSGGGMRTVFTGGVDERIKCAVCVGFMTT
                     WRDFVLNKSFTHTWMTYVPLLPNELDFPEILGLRVPLPTMVLNDNDDDLYTLPEMKNA
                     DEILREIYDKAGAPEKYKCSFYPGHHKFDAEMQKEAFDWFDRWLK"
     gene            10138..12507
                     /locus_tag="PK288_06815"
     CDS             10138..12507
                     /locus_tag="PK288_06815"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPM18670.1"
                     /translation="MKRARLADTTKRGDLKCGSRFDPGINTGRIKRIFTLLFYTMLSL
                     SVLVSCREKQSLSGLVNVFIGTGGHGHTFPGATVPFGMVQLSPDTRIDGWDACSGYHD
                     DDRTIIGFSHTHLSGTGIGDYGDILIMPVSGREALVRGDEKVPGSGYRSGFSKSNEKG
                     SPGYYSVILDDYGIRAELTATTRAGFHRYTFPESDESGMIIDLDHTLQSHENLLLRIK
                     VISDTEIEGVKITRGWAKYHPVYFYAEFSKPFSYVLAVNNVPDSSLKEAEGRNLKALI
                     SFGTAKDEQVLVKVGISSVDIEGARNNVVTEIPGWDFEKVRFQAQEAWDRELGKIKVE
                     GGTDDQKVIFYTALYHSMISPNIFSDADGRYFGMDHKIHNSDGSLNYTIFSLWDTFRA
                     EHPLLVLINPSRDAGMIRSLLRKSDEGGLLPMWDLASNYTGTMIGYHAVPVIVDAYVK
                     GIRDFDAEKAFRAMVRASDFDTTNINAADRDILMLLMPLAKYYNNTLGYIPADKDNQS
                     VAKALEFAYDDWCIARMARELGKEKEYGIYSERALRYKKYFDPVTGFMRGRMSSGEWR
                     TPFNPRFSDHFHADYVEGNAWQWTWFVPHDPAGLIDLMGGKERFTAKLDSLFTVESTI
                     EGENKSADISGLIGQYAHGNEPSHHIIYFYNYAGQPWKTQKLADQILNTLYFNNPDGL
                     SGNEDCGQMSAWYVLNALGFYPVAPGDPSYSIGRPLFDKAVVSLEDGRKFTVTALNNS
                     AENIYIQKITLNGRPLDIPFFAHKDIAGGGEMVFEMGPSPSLTWGLMAK"
     gene            complement(12542..15181)
                     /locus_tag="PK288_06820"
     CDS             complement(12542..15181)
                     /locus_tag="PK288_06820"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPM18671.1"
                     /translation="MKRIPIYCLILILIFPGVTGQTSGKKQYRAEKLLSAPVIDGILD
                     DEAWMNEGTWIDDFTQYEPYNGGRSSQKTEFKILFDNNNIYVAIRAFDTSPDSIVRRL
                     TRRDNSDGDMVAIIFDSFHDLRTGFLFGISSAGVKYDQVFTNDGQTEDSSWDPNWWVK
                     TSVNPEGWVAEMKIPFSQLRFEKNSGDIWGLQVARVLYRKRETSFWQHIPKDAPGLIH
                     LLGELTGLREIKPGKIFDITPYGVARGETYKADPANPFLQKGRRYGFNGGIDAKIGVT
                     SNMTMDLAINPDFGQVEADPSEVNLSAYETFFREKRPFFIEGNNITNFNLGIGNGDVG
                     NDNLFYSRRIGKRPRGNPDLDEGMYADIPRFTTILGAAKLTGKTGKGLSVGFIEAVTA
                     EEKAEIRKSGERSYETVEPLTNYLVGRVQKDLDEGKTIFGGIFTGTNRDLDGNLAASM
                     HRSAYTGGLDFTRYFRDKSWMLSVSTAFSQVSGTREAIAETQKSSARYFQRPDKEYAR
                     FDPDRKSLTGTGGRLQLMKLDGHLNLMGVMTWKTPGFEINDLGYSQNSDQILAVLWAG
                     YREWEPRGIYRRYNINADFFSSWNFGGINVGKGFETNANMTLKNYWNLWTGGNIETGS
                     VSTGILRGGPMMKLPGRFHWQFGFSTDNRKKLGFQFFSNYNKGFKNYSRSTYTSLELS
                     YKPANYLSFFLGPAYNRSFSELQYVTTLQFNNNDRYIFAGINRETISASFRINFNLKP
                     DLTLQYWGQPFIATGRYNSYKYITNPLADSFTGRFRTYTDEQITQGVDYFEIDENSDG
                     IADYRFNRVDFNVREFLSNLVIRWEFSPGSTVYLVWSQNRRGFDNSGKMHFYNDLGNL
                     FSTGTDSYNNVFLVKFSYRFGLR"
BASE COUNT         3917 a         3508 c         3809 g         4146 t
ORIGIN      
        1 gccaccataa acggggctcc gatatcacca gctggctgaa gtccgaagga tcaaaaacta
       61 ctttcttcga ttccttcggt ccgagtgtta cttcctgtgt aaaaacaata ttgtcaatgg
      121 tgcctttaag cttacctttg atcttcccgt cagtaaggtt ttcaacttcg gatgcaatgg
      181 tcaggcgggc ctggtcagtt gagggaaggt tgagtttggt gatcacctgc gggtttttca
      241 ctgtcaccgg ccctgttgac cgtaccgtaa catcatacca gatacccatt cccctgcccg
      301 ggggcgtggg gttccagtcg acccaggcaa ttgttagatc gaatctttcg gggggaaata
      361 tttcaagtgc aaggcagttg atttctcccg gttttgcata atcagtaatg ttcaggtcat
      421 agatgcgata ggctccctgc actgaggcgg tgtcagctat caggctgccg ttgagccaaa
      481 tatttgcctt gtagttaatg ctgtggaacc tgatccatgt gtttttagct ccgttaccgg
      541 ccggaagatg aaattcggtc ctgtaccacc atgagacttt aaagggacta tcgtcaggaa
      601 caataggggg ccattttgta atgtagcccg ggattttgag gatgtttgtg ccgaaataag
      661 gatcgggata aactttgttc tccacaaggg ttccaagcac agttgaggga acggttgcag
      721 ggtaccagtt ctccggtttg aatgcagttg tcgaaataac gctgccggat tccccgacag
      781 cagctgatga ctgtatcgcc cagttctccc ttaacaaaac gggttcctgt ggttcaggcg
      841 gccgggaaca ggcaaaggat acaattactg aggcaaggaa aactaatggc aggcaatgtg
      901 ttttcatggt tattatgggg tttttactgg tcttccgcgg taaaataaat cctgaaaagg
      961 ggatctccct ttacgggaac tagtgaatcg ggtgtaatct caatgacgct gaacctttca
     1021 gcaggtatgt ttcgttcggt ggcaaggtat tttttcaggt atccggcatg tcctgctctc
     1081 agggaatcga gagatgaact aagtacatct tcgccaaagt attgcctgga aagggctgcc
     1141 agcggcaagc tgtccgttcc gtcagggagg gttaatttat tcctgagata tctgaacagg
     1201 gatgaatcgg ctatgttcct tatatccttt tcccggagga tgcctgaatt catcagttct
     1261 ttcctggcca ttatgcctgc cagagtgtcg gctgccctgc cgcgattgat gcagtagata
     1321 aaatctgctt tcagcattgg cttcttgtcg aggatacctg ctattgcgtc aagtgaccga
     1381 atcgcttttt cgtccggctg aaactcagtc aaattcaacc tgactgactg aagtgaggaa
     1441 ggatcggact gaatcagtcc ggaaagcaga tttatggggg aaactgctgc tttaacaaag
     1501 aggtttccaa ttatccggaa aatgattttc cccagattga caatcctgac ctcttctcct
     1561 ttcatcctta cgggagcttt cagatcgata atcccgtttc tgtcgctgag tactgccagg
     1621 gcaaggcgaa gggggacatt gaaccgggtt tcatctgcga cttttttgcc aaggctgaat
     1681 ctcctgaagt agatttcatt attgcttaca agcgattttc ccttcagttt gttgtcggtt
     1741 cgtaaattca ttgttccgcc ggtaacggga aatccaaggt aatgtctgaa gtatgcatcc
     1801 agatccttca tccggagcct gctgatatcc agtgaaagct ccacgtcact gccggggttt
     1861 tcaaggctga acattccgtg tgtaatcagt ttgccggttc cgttcagtcc ggccgcgaca
     1921 tcgtaggata ttcttccggg ttgatctgtg atttccttaa catttagttt gagactgtcg
     1981 attttatatt caaacggata gcggagattt ttatccatat acaggatcga tccgccgctg
     2041 accgaaactt cagggatatt atacctgaat cctcccgtta taccggtggt gtcggcgctg
     2101 atcgcggcag aatccgcact ttccccggtt ttcatcagtg tggtccagtt ggttaccgag
     2161 tcctgcagtt ccataagcac gaatggatct gttagactga tactgcctaa atgcaggcgg
     2221 ccggcggaaa gatcaagcgt atcaattttc acggaaagat cgttgaatga gaggatggtt
     2281 ctcccgaggg tgtcctgcag tccgaaatta ctgacctggc tggttccctg aacggaaagg
     2341 ttaagtatgc tgctgatact tccgtttatc atgaggtcgc tggtgaggtt gccggttagg
     2401 gttgacacat tcagataatt cctcacatag ggttctgtga tccccaggtt caggctgtcg
     2461 atcatgaggc tgagcctgta ggtgctgtca gcctggttga tctcaaggtc agaacttatg
     2521 ccaccccctt caacaaacct gaaacctgcg ccaagcctgg ccgaatcgct gttccaggtg
     2581 aacccgggaa tcgtcaggtc aatactgtcg agatagatgg tatgctgcag gggaacatcg
     2641 gtgtatttca cgaaaccgcc ggcaatcctg atattatgga ttacatattt tgccggggtt
     2701 ttccggacgt tttccctttc attttctgtg gagtccgggg ccacaagatc agaaaagttg
     2761 aacatgctgc cattctgcat cacctgtata aacggatcat cgagcgacac ctttctgatc
     2821 acgaattcat tccgtagaag aggcaggtaa ctgacagaga tcctgaactg cctgaagctt
     2881 gcaaaaaccg ttttttcgtc ggcttcaaat attttaaagc ccctgacctt cagggtacca
     2941 gtcagataac gaatcccgat tctgtcaatt gtgatccggc gcccgatcag ctccatgctg
     3001 tttttctcaa tataccatcg ggcgatcccg ggaactgcaa gaagcaataa ggcaacagaa
     3061 aggaatatgc aggctgttat cagatatctt ttctttaaaa tcctgtacct cattcttaat
     3121 gctgtcttgt tatgggattg gatccggagt gcttttcctg cttaagggat gtgtcataag
     3181 cagatttact gtcatgtaca aagttaatca aaatctgaaa aaagctccgg accttaaaaa
     3241 cgtctaaaag gtaatcgggg aacagatgtc atcctgatct tccttcatga cctgtaaaca
     3301 tgattctggt ctgaattaag attcagacaa tccggttttt attttttcca tttcatattt
     3361 tgaactttta ttggattttg caacaattca ttataactta cagattcttc ttttgcctac
     3421 caggaatata acttaaacca gataagaaat gaaaacaagg aaattcaccc gccgccaggt
     3481 cattgcggca acatcggcag gcttcttagg ttcaatgctg aattattcgt tcagaccttt
     3541 taccgtggca ggtacaagcc agtcggtact ggcaatcaac ggaggagaaa aggtccacaa
     3601 gggaccatgg tccgactggc cgttctggga caggagtgcg gaaccgggta ttctggaaat
     3661 gttccgttcc gggcgatggt ggagaggtga aggggaacat gttgccgaat ttgagaaaaa
     3721 atacgccgaa cttatgggtg ccagaagctg ccttgcaacg gccagcggca caacttccct
     3781 gattattgcc atggaggcgc ttggtgtcga tgcgggggat gaggttctca tatcgccgta
     3841 tacattcatt gccacataca attctgtatt tgctcacaag gcactgccgg tatttgttga
     3901 ttcagatcct gcgaccttcc tgatggatcc ggcaaagatt gaagaaaaga tcaccgacag
     3961 gaccgtggcc ataattcccg ttcacatata tggcctgccg gttgatatgg acagtgtcaa
     4021 ttccgttgcc cggaagcatg gccttaaggt tattgaggat gcctgccagg catggcttgc
     4081 tgaatacagg gggaaaaaaa caggcaccct gggtgacctg ggatgcttca gtttccagaa
     4141 ctcaaagcac ctgcctgcag gtgagggcgg agccatcctg agcatggatg aggctcttgt
     4201 cgacaagtgc tatgcgattc ataattgcgg caggccaaga ggtaccatga aacccggtcc
     4261 gggatatccc taccggggaa gaaattacag gatgcagcaa tcgcaggctc tgatcctgtt
     4321 gtctcagatg aaaaggattg aaaaagataa tgacgtccgc ctttcaaacg cattgtatct
     4381 tgacagaaaa ttgcgggaga tccccgggat aataccgtac cggccggcaa acggagccac
     4441 aagggcagtc tatcatcttt atccgttcag atatattaag gaagaattca ataatattcc
     4501 gaaagaaaaa ttcattgagg cacttaatgc agaaggaatt cccgctacag gcggttacgg
     4561 gaagcagaat aaggacggac ttatggaaga ggccctgacc tcaaggggct ataaaaggct
     4621 cttttcggaa gcaagactca ggcagtggag agagcagaac aatcttccgg gtaatgacca
     4681 gctttgcgat caggcagtaa cattatacca gagcatcctt ctcggatcga aggccgatat
     4741 ggatgatatt gttaatgcaa taacaaagat ccataagctt aaaaacacac tcagaccgtg
     4801 atttgttata tttgcagaat cacaccgtga gaatgaagat tttaaggccc gttgctgcag
     4861 cagtattact ggtttcatta gccgcatgca agcactccgc tgaccggagc atcctgtatg
     4921 aacggatggg aagactttta gaagaaggtg aatttgcaga acttgagaaa ctggcggatt
     4981 ctcttaagac cctgagactg tcagacaacg atctggttgc caaagccgat tcctttgttc
     5041 agattgccgg cagaatcgca attgattttt cagtgccgga agctgatttt attgatgatc
     5101 tgaaaaaaag aactggaggt tattccctgg aggaattcag attatgggag aaaaacaact
     5161 ggctcgaaag gaggaaaatt aatggcgaaa taagatattt caggcgggcg gcctcaaacc
     5221 tggtccgact cagggatttt aacctgcgca gggcttattt tgattcgctg aattccgtat
     5281 ctcctgaaat caggttcaga ttgaagcata ttgaatcggt catcagggcg gcccgggaaa
     5341 attccgcacc tgtggttccc gtggaaatga caattaccta ctcgcttact gtaaagtccg
     5401 atgctgtgcc ggaaggagaa attgtcagat gctggctccc ttacccgcgg gaagatcaac
     5461 cccggcagca gaaagtgaaa ctcatttctg cctctcatgg aaattacatt gtctctcccg
     5521 attcagttac ccacaggacg atcttcatgg aaggaagggc tgtaaaggga atacccctcg
     5581 ttttcaaagt ggaattcagc tatcagttcg gagcgcaata ttatgatccg gataatataa
     5641 ataatctgcc ttcttcgggt tattcgggga tctgtgataa atatatctcg gaacagttgc
     5701 cgcatatctg tttcacggaa agggtgaaaa aacttgccga cagcattgcc gggaatgaaa
     5761 aggagccctt caggatactg aaaaagcttt attactggat tgacagcaat attccctggg
     5821 caggagcgct ggaatattcg acgattccct gcatacctga atatgtattg gccaacagga
     5881 ggggcgattg cggtatgaag actttccttc tgatgtcaat gttgagatac aagggtattc
     5941 ctgtcagatg gcagagcggc tggatgatgc ccccagacga tgaaaacctg catgactggt
     6001 gcgaagtttt tattgaaggt acaggatggc tgcctgttga catgtcgtac gggcttcagt
     6061 attcagatga caagaaaata aaggaattct atttgtctgg gatagattca tacaggctga
     6121 ttgtgaacaa tggtgtttca gggaagcttt ttcctccgaa gaaattcctg agaagtgaga
     6181 cattggactt tcaaaggggt gaggttgaat gggaaggcgg aaacttatat tttaatgagt
     6241 ggacctataa tatgggaatt gagtatacgg caaaaagcaa ttattccgaa aaaaggaacc
     6301 gtatgcagct taccccgtaa aaactcggaa cgaaatgaag caaacaagat taccctgttt
     6361 tttgttgctg cttgccggcg ggctgctgtc aggcatttca ttgcctggat gccggcagga
     6421 ctcgttggtt ttgttgtctg aaggactgga ttcgataggt gcttcagtgg ttaaggataa
     6481 aagggaaggg atctttaaca ttaatcttta cagggcaggg gaaaaaataa ttctcagcgg
     6541 tgagactgac agtcgtgatg cccgaaattc agtgttgggc taccttgagg ggaaaggagt
     6601 tcgttttatt gacagcctga tgcttttgcc cgatactgcg gcgcttggaa ctcagtgggg
     6661 tctggtgaat gtcagcgtgt gcaatatgcg gctggtcccg tcgtatgatg cagagctgat
     6721 ttcacaatcc ctgatgggaa cgcctgtccg gctccttaaa agggatcacg gctggtttct
     6781 ggtgcaaacc cccgaccggt atatcggctg ggtcgacagc gacgccattg cggtcttaac
     6841 cagtgaagaa tatcgagatt ggaaatcgtc ggaaaggatt ttcttcaccg gcaaaacagg
     6901 cgatatttac ggagatcctg aaaaaaagac gactgtatcc gatattgttg cgggatgcat
     6961 tttaaaggtc agcggcatca gcggcaggga ttaccgggta atactgcccg acggccgcag
     7021 cggattcatc ccggcttccg gtgcaatgat acttgatgat cttgatccag tcaggctgct
     7081 tacacccgaa acactgatat cacgtgcgga atcattcaat ggtatcccct atctctgggg
     7141 aggaacatcc gtaaaaggat tcgactgcag cggattcgta aaaacagtgt attacctgag
     7201 cgggattatc cttgcgaggg atgcatcgct gcagttcagg cacggaactg aaacggacac
     7261 agtaaatttt cccgcttcgc tgaagcccgg cgacctgctg tttttcggat cagtcaggga
     7321 aggccggccg agggccacac atgttgccat gtataccggc aatacagagt atattcatgc
     7381 ttcggggatg gtcaggacca acagtctcga ccgttcgcgg agcaatttca gcggataccg
     7441 gagagataca ttcattggag caaagaggat tatcggggca gggtatggcg agggaatcat
     7501 tcctgttgcc gggcatccgt ggtacaggta gaagatgaac aaaaggactt tcataaaata
     7561 ctgcacatct ctggcaggaa tatcattggt tggcagaaat tttgccggtg cagcaactac
     7621 tttcccggta aacaggtaca atatgaaaaa atcaggcact cttaagttgc ggtatgctcc
     7681 gtatacactc caactaaaac atgcatttac gcttgcaaca agttcaagga ccacaactcc
     7741 ggttgtactt acagaactgg aattcgaagg gatcacgggc tatggggaag cttctatgcc
     7801 cccgtatctc ggggagagcc acgaatcggt gctgaagttc cttgctgaag tcgatctttc
     7861 ccggttttct gaccctttcc tgctggagga tattctgatt tatgttgata atgtgatgcc
     7921 tggaaattat gctgcaaagg catcaataga tattgcactc cacgatctta tcggaaagat
     7981 cacgggacag ccctgttaca ggctgttcgg gctcaatccg gcaaagaccc cgctgacaag
     8041 cttcaccata ggtctggatt ccccggaaat tataaagcag aaagttgagg aagcggaacc
     8101 ttacaggata ctgaaggtaa agcttggccg tgacaatgac agggaaatga ttgaaaccat
     8161 cagatcactg actgacaaac cattgtgtgt tgatgtaaat cagggatgga ccgacaggaa
     8221 tcattccctt gaaatggcac actggcttca tgaaaaagga gtgatattcc ttgagcagcc
     8281 tatgccccgg tcaaggaagg atgatatagc ctggctgacg caaaacagtc ccttgcccat
     8341 aataggcgat gaagcgattc agcatgctga cgacattgtc agccagaagg atgtttattc
     8401 gggtgtcaac ataaagctca tgaaatgcgg cgggatgcat gcggcccgca atatgatcgg
     8461 ccttgcaagg gccctggggt tgaaagtcat gatcggttgc atgaccgaaa cctcatgtgc
     8521 cgtctcggct gcggcccagc tctccccgct ggttgactgg tgcgacctcg acgggaactt
     8581 gcttatcagc aatgatgtgt ttgatggtat gaagatcacc ggcggtaagg tgactcttcc
     8641 tgaccggccc ggtatcggag taataaaact gacctgaaag acgggaaggg attccggcat
     8701 tacagcctat ttcagccacc tgtcgaacca gtcaaacgct tctttctgca tctccgcgtc
     8761 gaatttatga tgtcccggat aaaatgagca tttgtatttt tccggagcgc cggccttatc
     8821 ataaatttcc ctgagaatct catcagcatt tttcatctca ggcagggtat aaaggtcatc
     8881 atcgttgtcg ttcagcacca tagtgggaag tggcaccctg agcccaagta tttcaggaaa
     8941 atcgagttcg ttgggtagca ggggtacgta ggtcatccag gtatgtgtga atgatttatt
     9001 cagtacaaaa tcgcgccagg ttgtcatgaa ccccacgcag actgcacatt tgatcctttc
     9061 atcgacaccc ccggtaaaga ctgtcctcat tcctcctcct gacaggcccg cgcatccgat
     9121 ccggctgctg tctacatcct tcctggaaca taggatatcg agggcaactg agtcttcagc
     9181 catgaacacc ccgggccaag ttgtgccagc gctgaaaagc gactttgcca ttatgtgctc
     9241 atgctcacgg gcccagttat tataggcatt tacgtttgcc ggatcctccg gatcatcgtc
     9301 agtcagtccc tgcttcagat gaaccggcac atcctgcatc atgaccctcc tgctcgcgaa
     9361 ggggaaagca tcggaaacaa gcacggcata acctcttctg gctatctcat ttgcccacgc
     9421 cctgccttca taatagtgct cctggtgatc tttcatcagg ggatgctgat cagctgaggt
     9481 ttttgtaatt ttcctggtac cgaaatactt gttgcctcca tgatcatgaa aggcaagaat
     9541 tccgggcagc tttcccactg cccctgccgg cttaaggaaa atggcgtcgg tgggccgtcc
     9601 gtagggaagc tgccatctga gctcctcgat atggagaccg tcgaaagtat agctttttaa
     9661 ttgctgaact tccgggcttt taccggtttc aggcatgacc acgcgctcaa aaagcctttc
     9721 ctttgcaaca gtcttccacg atttaacatc tttccattcc tttcgcctga aggacagtcg
     9781 gggaagattt ttccccgaaa gcccggcggc ccagctgcca tacacaccga tgagacttac
     9841 ctgatcctgc acggttatcg gcctttccat attcgtactt tccgtttcgg aattcatata
     9901 tatatcattt gatttgttat gccggtaatc gctattgatt ttctccggaa aatcaaaacc
     9961 tgccaaccct ataccaaggg tcccggcagc ttttaaaaac cccctccggt tgcttttcat
    10021 atttttgaga ttgttaaaat gcttaaattt acattaattg tttttaatca ttatttattc
    10081 gggttgaaaa agaaaaatac cttcaggaaa acgtattaac ccataaaaac aaatcatatg
    10141 aaacgagctc gtctggcaga tacaaccaaa cgtggggatc tgaaatgcgg atcccggttt
    10201 gatccgggca tcaatactgg caggataaaa aggatcttca ccctcctgtt ttatacaatg
    10261 ctttcactat cggtgctggt ttcatgcagg gaaaaacagt ctctttccgg acttgtaaat
    10321 gtttttatag ggaccggcgg gcatggtcat actttccccg gggcaaccgt tccattcggg
    10381 atggttcagc tcagccccga cacaaggatt gatggctggg atgcctgctc aggatatcat
    10441 gatgacgaca ggacaataat aggtttttcc catactcatc tgagcgggac ggggatcggt
    10501 gattacggtg acatcctgat catgcccgta tcaggaaggg aagccctggt aaggggcgat
    10561 gaaaaggttc ccggatcggg atacaggtca ggcttttcca aatccaatga aaagggttct
    10621 cccggctact actccgtcat tcttgacgat tatggcatca gggctgaact tactgctaca
    10681 accagggccg ggtttcatcg ctacactttt cctgaatcag atgaatctgg aatgataatt
    10741 gaccttgacc atacccttca gtcacatgaa aacctgcttc tccggataaa ggtgatcagt
    10801 gatacggaaa ttgagggtgt aaagatcaca aggggatggg cgaaatacca tccggtatat
    10861 ttttatgcag aattctcaaa accattcagt tatgttcttg ctgtcaacaa tgttcctgat
    10921 tcttcgttaa aggaagcgga aggccgtaat ctcaaggcac tgatttcatt cggtaccgca
    10981 aaggatgaac aggtgctggt caaggttggt atttcttccg ttgatattga gggagcaagg
    11041 aacaatgtcg tgacagagat cccgggatgg gattttgaaa aggtcaggtt tcaggctcag
    11101 gaagcctggg acagggagct tggcaagatt aaggttgaag gcgggacgga tgatcagaag
    11161 gtgatttttt acactgccct gtatcattca atgataagcc ccaatatttt ttccgatgct
    11221 gacgggcgtt attttggcat ggatcataag atccataatt cagatggttc gctcaattac
    11281 accatctttt cactctggga caccttcagg gccgaacatc ctctccttgt gctcatcaac
    11341 ccttcccgcg atgccgggat gataagatcg ctgcttagaa aatctgatga aggcggcctg
    11401 ctgcccatgt gggatctggc ctccaactac accgggacaa tgatcggcta tcatgcggtc
    11461 ccggtcatcg tcgatgcata tgtgaaggga atccgtgatt tcgatgcgga aaaggcattc
    11521 agggccatgg ttcgggcatc tgatttcgac acaaccaata tcaacgccgc agacagggat
    11581 attctgatgt tgttgatgcc gcttgccaaa tattataaca acactctggg gtacattcct
    11641 gctgacaagg acaaccagtc ggtggccaag gcccttgagt tcgcctacga cgattggtgt
    11701 attgcccgga tggccaggga acttgggaag gaaaaagaat atgggatcta ttcggagaga
    11761 gcgttgcgct ataaaaagta ctttgacccg gtaacaggtt ttatgagagg caggatgtcg
    11821 tcaggggaat ggagaacccc gtttaatccc aggttttctg atcattttca tgctgactat
    11881 gttgagggaa atgcctggca atggacatgg ttcgtgcccc atgatccggc cggactgatt
    11941 gatcttatgg gaggaaaaga gagattcacc gccaaacttg attccctttt caccgttgag
    12001 tccactattg aaggagagaa caagtcagcc gatatttccg ggcttatcgg ccagtatgcc
    12061 catggaaacg aaccaagtca tcatataatt tatttttaca actatgccgg ccagccctgg
    12121 aaaacccaga agcttgcaga tcagattctt aatacattgt attttaacaa tcctgatggt
    12181 ttatcgggta atgaggactg tggccagatg tcggcatggt atgttctgaa cgcattggga
    12241 ttttatccgg ttgcccccgg cgatccatcc tattcaatag gccgcccctt gtttgacaag
    12301 gctgttgtca gtctggagga cggcaggaaa ttcactgtaa ctgcattaaa caattccgct
    12361 gagaatattt atatacaaaa gatcacgctg aacggaaggc cgctggatat tccttttttc
    12421 gcgcataagg atattgccgg cggcggggaa atggtttttg agatgggccc ctcgccctcc
    12481 cttacctggg gattaatggc gaagtagaag atcttcagat aaataatata tcttcggaat
    12541 actatctcag tccgaagcgg taactgaact ttacaagaaa cacattattg taactgtcgg
    12601 ttccagtgct gaaaagattt cccaggtcat tgtaaaaatg cattttgcct gaattatcga
    12661 atccgcgcct gttctggctc cagactaaat aaactgttga tcctggcgaa aactcccacc
    12721 tgatgaccag gttggaaagg aactcccgta cattgaaatc aaccctgtta aacctgtaat
    12781 cggcaatacc gtcagagttt tcatcaattt caaagtaatc aacgccctgt gttatctgct
    12841 catcggtgta agttctgaac cttccggtga atgaatcagc tagggggttt gtgatgtatt
    12901 tatatgaatt gtacctgccg gttgcgatga acggctggcc ccagtattga agggttagat
    12961 cgggtttcag gttgaaattg atcctgaagg aagcgctgat ggtttccctg ttgatgccgg
    13021 caaaaatata cctgtcattg ttgttgaact gcagtgttgt gacatactga agctcagaga
    13081 atgatctgtt gtaggccgga ccgaggaaaa atgaaaggta attggctggt ttatatgata
    13141 attccaggct ggtataggtg cttctgctgt agtttttaaa tcctttgttg taatttgaga
    13201 agaattgaaa accgagcttc ttacggttgt cggtagaaaa gccaaattgc cagtgaaatc
    13261 ttccgggcag tttcatcatc gggccgcccc tgagtatgcc ggtacttaca gaaccagtct
    13321 ctatgttccc gcctgtccac aggttccaat agtttttcag tgtcatgttg gcgtttgttt
    13381 caaaaccctt tccgacattg attccgccga aattccatga agaaaagaaa tccgcattga
    13441 tattgtacct cctgtatatt ccccggggtt cccattcgcg gtaacccgcc cagagtacag
    13501 ccagtatctg atccgagttc tggctgtagc ccaggtcatt tatttcaaag cccggagttt
    13561 tccaggtcat cactcccata aggttgaggt gcccgtcgag cttcatgagc tgcagcctgc
    13621 cgcccgtccc tgtaagcgat ttcctgtcag gatcaaacct tgcatactcc ttgtcgggtc
    13681 tctggaagta gcgggctgaa gatttttgtg tttccgcaat ggcctccctc gttccgctga
    13741 cctggctgaa agccgtgctg acactcagca tccagctttt gtccctgaag taccgggtga
    13801 aatcgagtcc tcctgtatag gctgatctat gcattgaggc tgcaagattg ccgtcaagat
    13861 ccctgtttgt tccggtaaag atgcccccaa aaatggtttt cccctcatcc aggtctttct
    13921 gaaccctgcc cacaagataa tttgtcaatg gttcaaccgt ctcgtatgac ctttcgccag
    13981 acttgcgtat ctcagctttt tcttcagctg tgactgcctc gatgaatccg accgagagtc
    14041 ctttgccggt tttgccggta agcttggctg cgcctaggat agttgtaaat ctcggaatat
    14101 cggcatacat accctcgtcc aggtcaggat ttccccgagg tctcttacct attcgcctcg
    14161 aatagaacag gttgtcattt ccaacatcac cgttgccaat tccaaggttg aaattggtta
    14221 tgttgttacc ctcaataaag aaaggtcgct tttccctgaa gaatgtttcg taggcagaaa
    14281 gattcacttc tgacggatcg gcttcaacct gaccgaaatc gggatttatt gcgagatcca
    14341 ttgtcatgtt gctggtgacg ccgatttttg catctatgcc gccattgaag ccgtacctgc
    14401 gtcctttttg cagaaatgga ttcgccgggt cggccttata ggtttccccc cgggcaaccc
    14461 cgtatggagt tatatcgaag atctttccgg gtttgatctc cctgagtccg gtcaattctc
    14521 ccaggagatg aataagtccg ggggcatctt tgggtatgtg ctgccagaag ctggtttcgc
    14581 gcttcctgta taaaaccctg gcaacctgca gaccccatat atctccggag tttttttcga
    14641 agcggagctg gctgaaaggg atcttcattt cagccaccca gccttcaggg ttaaccgaag
    14701 ttttgaccca ccagttcgga tcccatgagg aatcttcagt ctgaccgtca tttgtgaaca
    14761 cctggtcata tttgacaccg gcggaactaa ttccaaaaag gaaccctgtc cggaggtcat
    14821 gaaaactgtc gaatattatg gccaccatgt cgccgtcgga attgtcacgc cttgtaaggc
    14881 gtcgtacaat gctgtcgggg ctcgtgtcga aagccctgat ggcaacatag atattattat
    14941 tgtcaaaaag tattttgaat tcggtctttt gagacgatct gcctccgttg tagggttcat
    15001 attgtgtaaa atcatcaatc caggtccctt cattcatcca cgcctcatca tcaagtatcc
    15061 cgtcgatcac gggtgctgac aaaagctttt ctgccctgta ctgtttcttc ccggatgtct
    15121 gtcccgttac acccgggaag atcagtatta agatgagaca atatatcggg attcttttca
    15181 tggaagatat tgcaatctgc ttaccttttg tctaatgacg ttcaagataa gaaaatgatg
    15241 caagataatg gtatttggat tcattcttaa caatcagcct gaaaaccggt cttatcggtt
    15301 tcatttgccg gaatttcccc gtttctttca tcataatata attttgattt taatggtatg
    15361 atgcccgcct gacccgtcgg
//
LOCUS       DAOOYG010000062        14779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4083_length_14779_cov_6.755094, whole genome shotgun sequence.
ACCESSION   DAOOYG010000062 DAOOYG010000000
VERSION     DAOOYG010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14779
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4083_length_14779_cov_6.755094"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1554)
                     /locus_tag="PK288_06825"
     CDS             complement(262..1554)
                     /locus_tag="PK288_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18672.1"
                     /translation="MKRICTANPHKYEDLSCGSNPDLKTVFFRKVKIRSFQMIFTAIA
                     ATLTLTVQEAGSIPAFARKYQISCQVCHSPAMPRLKPFGDDFAGDGFRLTEYESPRYF
                     IKTGDDMLSLFRELPVAIRMDGFVTYNFGNKGSVDFGSPFMLKILSGGELSDKLSYYF
                     YFIFNERGSIAGVEDAFIMYHDLLNTGINLYLGQFQVSDPLFKGELRYTIEPYKIYSS
                     APGTSSADLKYDRGIVVEKDFSTGTTVVGQILNGSGIGEAGSGYLFDKDKYKNFMLRI
                     SQSVGKSISIGLFGYAGREVIENFLLGQATSNIRMFGPDLTIDLDEKLIVNCQFVRRT
                     DSRVYTVEFPYTEKKDVVTSGAFAEVIFAPKGDMSKWYLTGLFNWVDSDFDALDYTSA
                     TLHAGYLLRRNMRVVGEFTQVMSGKNFGKVNVGVITAF"
     gene            complement(1589..2971)
                     /locus_tag="PK288_06830"
     CDS             complement(1589..2971)
                     /locus_tag="PK288_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HPM18673.1"
                     /translation="MRKKNYLKILMLAGPLSVILLLLPGFVLRMKAPEENLKSRYSYR
                     DFESAKKCGSCHQGIYEQWSQALMSQAYTHHWDEIEYFDLAVKHSEADPALKEVVDGC
                     NGCHTPVAFMGAREFPPPRPSANSMANESVSCEACHLVQSAQTDPPFNFSYLIRPGMT
                     KFAVREPAVESPAHKIVTNDFYKTTEFCGNCHNEKSPFDVWVKTTQIEWKEGPYAREG
                     VHCQDCHMPRGGPFLNALMTKPYNDARIHLFHGGHDPGKVRGTIELRINPDIREAEPG
                     ETVVFTVVLFNQKTGHKFPTGSVEDRIAWLQVEATDAKGKVYHLDVDKKGFEGEEYTI
                     SGDYLAYQDMGVPLGIPDFRGVQRDGIPAGNRIFRMPFFDPEGRMTIMQWNTASLGVD
                     YRIGPRETKVETFTFRLPYETASGEMKVRAALNYQLLVKPVADLLKVPDDESEIKLVN
                     EHFTTINVLP"
     gene            3360..3779
                     /locus_tag="PK288_06835"
     CDS             3360..3779
                     /locus_tag="PK288_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18674.1"
                     /translation="MVFQRHGPILVLTFISIFTLLSCESGKDPVYAGSWRSVSRVETG
                     DIFYRTIRTLILTRSTYEETYEIQRENSGLTVGILGMKGKIAFSRYYMIFRLEELGSC
                     VRDESDACTRTVQWFGEGSQYWNDNIPYFQKTVRGRI"
     gene            3888..5543
                     /locus_tag="PK288_06840"
     CDS             3888..5543
                     /locus_tag="PK288_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM18675.1"
                     /translation="MRLTNSFLFAIILISAGCKGVGTGSETIKADVIIYGGTSSAVSA
                     AVQVSRMGRSVMVVSPDRHIGGLSSSGLGYTDTGNKEVIGGIAREFYNLIYRHYQNPE
                     AWVWEKQSEYGNRGQGIPAIDGEKRTMWIFEPHVAEEAFETLIRENQVPVYRDEWLDR
                     EHGVVKGRGKIKSIRTLSGKVFKGKIFIDATYEGDLMAAAGVGYTTGREPCSLYNEQW
                     NGVQTTDNPYKHHDFTDLDISPYVVPGDPASGVLPRISTEDPGRQGDGDRRIQAYCFR
                     TCLTKHEANRVPFDRPDDYDSTRYELLIRIYDAGWRGTFNKFDEIPNRKTDVNNHGPF
                     SFDNIGMNYDYPDATYERRKEIIDEHISYQKGLLYFIANDPRVPADTREEMKKWGYSK
                     DEFTDNGYWPYNIYVREARRMTGDYIMTENDVLSKRVVPHPVGMGSYNLDSHNTQRYI
                     KPDGFVDNEGDVQLPAGKPYMIDLGSILPKKEDCSNLLVPVCVSSSHIAFGSIRMEPV
                     FMILGQSAGTLASLALERKLAVHDVPYGELRKKLLADGQVLEH"
     gene            5812..6321
                     /locus_tag="PK288_06845"
     CDS             5812..6321
                     /locus_tag="PK288_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HPM18676.1"
                     /translation="MKKLIFIPLMLFAVNAALFAQASATATATAVIVGPIGISNTANM
                     NFGNVAVGATGGTVILEPSTTAGRTATGGVTLPATTGTVAAAAFTVTGVAGYTYAITL
                     PSAALTISSGANSMTVDNWTSNPTPTGTLDGGGSQTLWVGATLHVSGSQAAGTYTSAT
                     PFTVTVNYN"
     gene            6356..6889
                     /locus_tag="PK288_06850"
     CDS             6356..6889
                     /locus_tag="PK288_06850"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HPM18677.1"
                     /translation="MIRLRTVFFSLLLLFVTGGRGLFAQSAPPGSATGHVIAEVIPVF
                     SATETSQLNFGRFSPGPEGGKIILTPQNTLSVVGSIFKGPGAFNAASFYVTGDSDGAY
                     SITLPAGPVILKHTSDAKTMVIDNWVSVPVAGTGTGKLQNGFQTVFVGATLNVGSLKD
                     NPVGIYTGSYNITFDFY"
     gene            6917..7810
                     /locus_tag="PK288_06855"
     CDS             6917..7810
                     /locus_tag="PK288_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18678.1"
                     /translation="MAHLKSQKATNFSGSCRTGPAWLKPAAGLMVLVSLFCGNVSGQG
                     DLLITPRRIVFEGPKRSMDLNLANTGQDTAVYAISLIQIRMKDDGGFETIAEPDPGQR
                     FADRYLRFFPRTVVLGPNEAQTVKIQLTRTNELTDGEYRSHFYFRSVPRIKPLGGEEK
                     PEDTTAISVRLIPVFGITIPAIIRVGESTSKVTLSGLSLGAGGDGMPMLEMVINRTGN
                     MSVYGDITIDHISADGKITRVGMANGVAVYTPNATRRFGISLMDVPGVDYGAGKLRVT
                     YSASSDVKPAKFAENELILNK"
     gene            7915..>14779
                     /locus_tag="PK288_06860"
     CDS             7915..>14779
                     /locus_tag="PK288_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18679.1"
                     /translation="MNKFSGNKQASAAFFLLFIFPAVSLGQTAGDYQSRGTGNWNTPG
                     TWQKYNGSVWQNNQGYPGQNPGTGTVTIRDGHTVTLNVSPTNPVGNLVIGEGSSGTLQ
                     TDNSNRILNTSGNLSVSDGATFNLGRITLTVSGTTSINGSLTDSHNNGSASFNGQFII
                     NSNGSFSTTNNSPFTFSGGITNNGTFSKGGSGTATFAGSQAINGSGPLSFSGGNVSIS
                     SGSSVTNSGIVTIAGVLNGDDAGSQWINGANATLNYGNGSVPMSTGSLVATATNNTVN
                     YNGTVNQSVASIIYSNLIISGSGTSTLTGEVTVENTITLASGTLSISANTLILNGSPI
                     AGSGSLNTTTTSSLVFGGTSSGVFVPSNVSNLRNLTINNPGGVTINNNLTLSSAGELT
                     LTSGILDIGSNTLSIANTAATAITNNSGAFVNITTGALRRTLPRNLSGTGNDYFFPVG
                     EGGVYKGLNLIDVNTGSPEPVLSVSVSPAGALDGDNITIGPVDSRYWTLIKVSGNLTS
                     ARIELFESGLSSQKTIGMSNSVSGPYVSIGGTLNPSSMTSSVVSGLSTFFCIGDKIRS
                     DKFYSYQTGNWNSVTTWTSDPSGTLQIGNEVPGNTDKAIILSDRTVTLTGNINTTDLE
                     LTIEPGGVLDQAGYRFTNTLAKLEGQGTLRLSSVNFPDAVTNTFITAGGGTTEYYNSS
                     DFTIPFSPSSYNNLIINCTGRVATLLGDLTLYGNLDVKAGNFRINDNISTTKLILTIN
                     GNVTVNNAASVTVGNGATNPAIGSAGLGGSAPFLNYYLNFHTVIIRGDFTNNGTVRFT
                     NLTYPLYTAFPPASAGPTSGAASVYFQGAADNTLTCNGTTDFYNLIVNKGTDQTYKLT
                     INSSEYDNFRLFGANSLATESVSENPLLKKALWIHTGTLVLKGKLIIPSLTEGTAPNA
                     HYYIPSNGALVTDGIDVVIFTTADDYREVNTSYGVAAPNNSTIGITTGGNSSLYVFGK
                     LQINNGILSTRESGGIVTSSTASGQIIINGGTVDAKQMLGSTGSASYTQTGGLFILRG
                     RLQRIPVSYVTIADITDISNASLGTSRINNGISTAHGSFNLENPANIYSVSGGIIRIY
                     DVTLNTGGKAFDVKSSLSNINVTGGSLEIMPVAGTVLADATSFLIYSNAPIYDLSVNR
                     LGSTSVAGLSTPLNVLNDLNLISGSLTANNFNLGVGGDFTIENGAAYSPGTNTTIFNG
                     SGNQVFTINTGAALSLNALTLNKQAGDSIILAGSQNIINAAGSFNLTLGTLVDGGKTL
                     NLSGGTIINSGIHTGTGIINLNGTAAQSIDGNGTFQNISLNNTSGTPVSLAANMTING
                     TLTFSNNALFNIGTYNLRLNASATIVNYGANRYILTAGNAGDGGLTRVYSSTDALVFP
                     VGAPTITPARAAKYTPATLGFTSAPSTYGSVTVIPVGYENPNTTIKGQSLTYYWRIKS
                     SGFSGIPLYSVTHTFVYGQPDVVGSEAAYAPALYDETAHLWYYGTTSSVNTTTNIISD
                     WTTPTNSTNFLDGNYTAGVPSGFGTPRIYYSRRSGPWANVSTWSLTGHTVDNPPAAVP
                     GPNDIVMIGDRDSVYLATHNTNPNTGVQNCATLLIEAGSALDIGYNPACRFAMVLSHP
                     SGNGNFRLTTNNNTGAIFAFPSGDFSDFNANRGTTELYTTNPTAGTTYYMQTGISSYG
                     NLILSPLGGSNLIFPNNDVTIYGNLITRGQNADSWFVPAWQTTYPGNVQVIPKTITIM
                     GNMDIQGGALIWYGSGNRAQDFVIHGDVKIGVYASLYVYSGATNQSMAIGGNLINDAN
                     GQINGSGVTTVSKADFNNIPLTFFGPNSSAVTNTTGMPSTTFGSVTVNKGSSQETTLT
                     INIAGTLNTPANNWLNLLNGTLIYNRVGNLTISQGTDFTIPATSGLVINTPSNVYIAN
                     NNAINRTLFLDGRLAITGGDGNVYIGPAGNSTNNADIEYSGSGASQLDIQAGNLYVNG
                     QIRRPLASTNGILNYSQTGGNVYIFGNNPTTTNLTKAKLEVLNEGSSFTMTGGILTIV
                     RGGGTTFGDLYLRPSSSSVTGGTIIFTQVPSSGPVIDAPQSYSLDANIPLNNLTITGK
                     TAPTRRAATLALMVSSLSLNGSLTLTNSYSIFNSNDLNISLKGDLNNNGTYNYGTNLT
                     TFNGGAQFITGSSTSDFHDLDVLSLTTLTVSNSFTVGRNLRISNGDLVLGSNRVTVIG
                     NLVNNGSYTDNNSSGGITLAGTTRQQISGTGLFGLLDINNTEGARLYSDINLRNNLVL
                     SNGVLDINMYLLTLSQNSIISGAPFSDHKMIMPDGVVSSP"
BASE COUNT         4072 a         3527 c         3515 g         3665 t
ORIGIN      
        1 accggagcgc cggcagcagc atcagccggc agcagccaaa gagcctcgaa aggaacagca
       61 gtaagggaaa gcagaaccag cagtcagggc aggaatccag aaagcagcag ttcgcggagg
      121 aaataaaagg atcatttcat tcaaaataat taccaggaag gtatccgcac gggatacctt
      181 tttttttatg tcctgcccgg aaaagtatga cttgccggaa ctatttccgg ctgattgtta
      241 atcttttaac aattgaccgt atcagaatgc agtaatgact cccacattta cctttccgaa
      301 attctttccc gacattacct gtgtgaattc ccccacaacc cgcatattcc tccggagcag
      361 gtatcccgca tgaagcgttg ccgaagtgta atcgagggca tcaaagtcag aatcaaccca
      421 gttaaacaat ccggtaaggt accacttgct catatcacct ttcggagcaa aaatgacttc
      481 tgcgaatgcc cctgatgtaa ccacatcttt tttttctgta tatggaaatt ccacggtata
      541 aacccttgaa tcggtcctcc taacaaactg gcagtttaca ataagttttt catcaaggtc
      601 aattgttaaa tcaggcccga acatccggat attactcgtc gcctgaccaa gaagaaaatt
      661 ttcaatgact tccctgccgg catacccaaa caggccgatt gaaatgctct tcccaaccga
      721 ctggcttatt ctcagcatga aattcttgta tttgtccttg tcgaacaaat aacccgaccc
      781 tgcttcccct attccggagc cgttcagtat ctgtccgacg acggtggttc cggtgctgaa
      841 atctttttcc acgactatcc ccctgtcata cttcaggtca gcggaagaag taccgggagc
      901 cgaactgtaa attttatatg gttcaattgt atacctgagt tctcccttga agagaggatc
      961 ggagacctgg aactgaccga gataaagatt aatgcccgta ttcagaagat cgtggtacat
     1021 gataaacgca tcctccactc cggcaattga tcccctttca ttaaaaataa agtagaaata
     1081 gtaggacagt ttgtctgaca actctcctcc cgacagtatt ttaagcatga aaggtgatcc
     1141 aaagtcgact gatcccttat ttccgaaatt ataggtgaca aagccatcca tcctgattgc
     1201 aaccgggagc tcccggaaaa gggaaagcat gtcgtcgcct gttttgataa aataacgggg
     1261 tgattcatat tccgtgagtc tgaaaccgtc accggcgaaa tcatctccaa atggtttgag
     1321 ccgcggcatg gccggtgaat ggcagacctg gcagcttatc tggtatttac gtgcaaaagc
     1381 cggtatggag ccggcctcct gaactgtcaa tgtaagtgtt gcagcaattg ccgtaaagat
     1441 catttggaag gatcttattt tcaccttccg gaagaaaacg gttttcaaat caggattgct
     1501 cccgcatgaa agatcttcat acttgtgagg atttgctgta catattcttt tcattggatg
     1561 atatttttac tcgttgatga caacagattt acggaaggac gttaatagtt gtaaaatgct
     1621 catttaccag tttgatttca gactcatcat cagggacctt cagtaaatcg gccacgggtt
     1681 taaccagcag ctggtaatta agtgcggctc tcaccttcat ttcgccggat gccgtttcat
     1741 agggaagcct gaaggtgaat gtctctacct ttgtttcgcg cgggccaatc cgataatcaa
     1801 ccccaagtga tgctgtgttc cattgcatta ttgtcattct gccctcggga tcaaagaaag
     1861 gcattctgaa gatccggttt cccgcaggga tcccgtcgcg ctgcactccc ctgaagtcgg
     1921 gaatgccaag gggaacgccc atatcctggt atgccaggta gtcgccggag atagtgtatt
     1981 cctccccttc aaagcctttc ttgtccacat cgagatggta aactttgcct tttgcgtcgg
     2041 tggcctcaac ctgcagccat gctattctgt cctccaccga ccctgtaggg aacttatggc
     2101 cggtcttctg gttgaaaagc acaacggtaa ataccaccgt ttcaccgggt tctgcctcgc
     2161 gaatatcggg gttgatcctg agttctattg tgcccctgac tttcccggga tcatgtccgc
     2221 catggaagag atggatcctt gcgtcattat agggctttgt catcagggca ttcaggaagg
     2281 gcccgcccct tggcatatga cagtcctggc agtgcacgcc ctccctggca tagggtcctt
     2341 ccttccattc aatctgggtt gtttttaccc atacatcgaa agggcttttt tcgttgtggc
     2401 agtttccgca aaactcagtg gttttataga aatcgttggt cacaatttta tgtgcaggtg
     2461 attcaactgc cggttccctg acggcaaact tggtcatccc gggcctgatc aggtagctga
     2521 aattgaaagg agggtcggtc tgggccgact gaaccaggtg gcacgcctca catgaaaccg
     2581 attcgttagc catgctgttg gctgagggcc tcgggggcgg aaattccctg gcacccatga
     2641 atgccaccgg ggtgtggcag ccattgcaac cgtcaaccac ctctttcagt gccggatcgg
     2701 cctcggagtg tttaacggca agatcaaaat actcaatctc atcccagtga tgagtatagg
     2761 cctgcgacat cagggcctga ctccactgct cataaatacc ttgatgacat gagccgcatt
     2821 ttttagccga ctcgaaatcg cggtacgaat acctgctttt aaggttctcc tccggagctt
     2881 tcatccgcaa gacaaagccg ggcaacagga ggagaattac cgaaagcggt cccgccagca
     2941 tcaatatctt taaataattt tttttccgca tatgccgata ttttaagttc agaatgactg
     3001 taaaagggtg taacctcttt tttcgtggta aatttcacgg gggtgcatcg tggaaatgcc
     3061 ttaaaccggt cttcttatta atcgttatca aaacaaagtc agcagttctg aaagagatca
     3121 ggtcagaaat catttacaat ttagcaatat ttcaaattct gttatttcaa atatgccaaa
     3181 aatgctgaaa tttctgcagc atatttgcct gccgggaaaa atcaacaata caacttacag
     3241 gatattatat ttattgatcc ggtcagccag gaaagttaag taaaacataa tcacgatcag
     3301 aaaggagttt gtacaaaact ttttgtaatt ttagcattaa gacttaaatc ccgtgaccaa
     3361 tggttttcca acgccacgga ccaatcctgg tcctgacatt catcagtatt ttcacattat
     3421 tgtcatgcga atcaggaaaa gatccggtgt atgccggatc gtggagatca gtgagcaggg
     3481 tggaaaccgg ggatattttt tacaggacaa taagaacact gattcttacc aggtcgacct
     3541 atgaggaaac ttatgagatc cagcgggaaa attcagggct gacggtcggg atcctgggca
     3601 tgaaaggcaa gattgcattt tcacgatatt acatgatctt caggcttgag gagcttggct
     3661 cctgcgtacg ggatgagtcg gatgcctgca cccggacggt acagtggttt ggagaaggca
     3721 gccagtactg gaacgacaac ataccatatt tccagaagac cgtgaggggg agaatttgaa
     3781 gcgggtgaga caagattgca tctgaggcgg gatctgaata atgacaatga caccgatgat
     3841 ctgggcgaag atattgaatt tgaaagaatc tgataaataa ttcggggatg agactgacaa
     3901 acagctttct ttttgcaatc attttaatct ccgccggatg taagggcgtg ggaacgggat
     3961 ctgaaacaat caaggcggat gtaattatat acggcggcac gtcctctgcc gtttcagcag
     4021 cagttcaggt gtcgaggatg ggcagaagcg tcatggttgt ttcgcccgac aggcacatcg
     4081 gggggctttc atcttcgggg cttggttaca ccgacaccgg caacaaggaa gtgataggcg
     4141 gcattgcacg ggagttttac aatcttattt accgtcatta ccagaaccct gaagcatggg
     4201 tatgggaaaa gcagtcggaa tacggcaaca ggggccaggg cattcctgct atcgacggtg
     4261 aaaagaggac aatgtggatc ttcgaaccgc atgttgcgga agaagctttc gaaacgctga
     4321 ttagggagaa ccaggtaccg gtttacaggg atgaatggct cgacagggag cacggtgtgg
     4381 ttaaagggag aggtaagata aaatcaataa ggactttaag cggaaaggta ttcaaaggga
     4441 aaatctttat tgacgcaacg tatgagggag atcttatggc cgcagcggga gttggataca
     4501 caaccggcag ggagccctgc agcctttaca atgaacagtg gaacggtgtg cagactaccg
     4561 acaatccata caagcatcat gatttcactg atctggacat cagcccctat gttgttccgg
     4621 gcgatccggc gagcggtgtg ctgccccgca tatcaacaga agatccgggc aggcagggtg
     4681 acggagacag gagaatacag gcatattgtt tcaggacctg tcttacaaaa catgaggcca
     4741 acagggtgcc gtttgacagg ccggatgatt atgactcaac ccggtatgag ctgctgataa
     4801 gaatatatga tgcgggatgg aggggaacct tcaacaagtt tgatgagatc cctaaccgca
     4861 agacggatgt aaataaccat gggcccttca gcttcgacaa tataggtatg aattatgatt
     4921 atcccgatgc cacctatgaa aggaggaagg agatcattga tgagcacatt tcctaccaga
     4981 agggattgct gtatttcatt gccaacgatc ccagggttcc cgccgacacc agggaagaaa
     5041 tgaagaaatg gggatattca aaggatgaat tcacagacaa tggttactgg ccgtataata
     5101 tttatgtaag ggaagcccgt cgcatgaccg gggattacat aatgaccgaa aacgatgtcc
     5161 tcagcaaaag ggttgtacct catccggttg gcatgggctc atacaatctc gactcccaca
     5221 acacccagag atatatcaaa ccagacggtt ttgtcgacaa cgagggagac gtacagttac
     5281 ccgcaggtaa gccgtacatg atcgacctgg gttccatcct gccgaagaag gaggactgca
     5341 gcaacctgct ggtacccgtc tgtgtttcca gttcgcacat tgcattcgga tcgataagga
     5401 tggaaccggt tttcatgatc ctaggccaga gcgccggaac actggcatcg cttgcacttg
     5461 aaaggaaact ggcagtccac gatgttccct acggtgaact caggaaaaag ctccttgccg
     5521 acggacaggt actggagcat taaaatcaca ttttttagga taaatactta acggaagcac
     5581 attcccggca ttcttcatgc ctggtttcga atggcatgca attccatgtt gataaaaaaa
     5641 aggaattgtt aaataataat aaaatgagat acgtgccgtt aagtcaattg gttattaatt
     5701 tggctgtggt aaccgggcga acaaatcagt cagggcggtt tcccggtaat gatcagctaa
     5761 atccaatcaa gacaataaac attaattaga aattttttaa actaaaagaa gatgaaaaaa
     5821 ctgattttta ttccattgat gctttttgca gttaacgcgg cattgttcgc acaggcttca
     5881 gccacagcta cggcaactgc agttattgtg ggtccgatag ggatatcgaa taccgcgaac
     5941 atgaattttg gcaacgttgc agtcggtgct accggtggaa ccgttatcct tgaaccttca
     6001 actaccgctg ggcgcactgc aaccgggggc gttactctgc cggcaacaac cggaaccgtt
     6061 gcagcagctg cattcactgt aacaggcgta gcaggctaca cttatgcaat aaccctgcct
     6121 tcagcggctc taacgattag ttcaggagct aacagtatga cagtagataa ctggaccagt
     6181 aatcccaccc caacgggaac cctggatgga ggcggaagcc agactctttg ggtcggagcc
     6241 accctccatg tttcaggtag tcaggctgcc ggaacctata catcggcaac cccctttacg
     6301 gttacggtaa attataacta gaataacagg aatcagtcag cgttacaccg atataatgat
     6361 aaggctcaga acagtatttt tcagcctgtt gttgctattt gttacaggcg ggaggggttt
     6421 atttgcccag tcagctcctc ccgggagtgc aacgggtcat gtcatagccg aagttattcc
     6481 ggtgttttcc gcaaccgaaa catcccagct gaatttcggg aggttttccc ccggccctga
     6541 aggaggaaaa atcatcctca ccccgcagaa tacgctgtcg gtggttggaa gcatattcaa
     6601 gggacccggg gcattcaatg cggcaagctt ttatgtaacg ggcgactccg atggcgcgta
     6661 ttcgataacc ctgccggcag gtccggtaat cctgaagcac acctctgatg ccaaaaccat
     6721 ggtcattgac aactgggtgt cggtcccggt tgcagggaca ggaacgggaa agctgcagaa
     6781 cggtttccag actgtttttg tgggggcaac cctcaacgtg ggcagcctga aggacaatcc
     6841 ggtgggcatt tacacgggat catacaatat aacctttgac ttttactagc tgtctatcct
     6901 taatccctga cataaaatgg cgcatttaaa atcacaaaaa gcaacaaact tctccggatc
     6961 gtgcaggacg ggtccggcat ggcttaagcc tgctgcaggg ctcatggtcc tggtttcatt
     7021 gttttgcggc aatgtttcag gccagggtga tcttctgatc acgccccgga ggattgtttt
     7081 cgaagggccg aagcgatcga tggatctgaa cctggcgaat accggccagg atactgcagt
     7141 atatgcgata tcgctgatcc agataaggat gaaggacgac ggaggattcg agaccattgc
     7201 cgagcctgat cccggtcagc gttttgccga ccgttacctg agattttttc cgagaacggt
     7261 ggtccttggc cccaatgagg cccagacggt gaagatacag cttacccgga ccaatgaact
     7321 gacagatggg gaataccggt cccattttta tttcagatcg gttcccagga taaagccgct
     7381 cggcggggag gaaaagccgg aggatactac agccatttca gtcaggctga tacctgtgtt
     7441 cggtataacc attcccgcga taatcagggt tggtgaatcg acgtcgaagg ttactctgag
     7501 cgggctgtcg ctcggggccg gaggagatgg aatgcccatg cttgagatgg tcataaaccg
     7561 taccggcaac atgtcggttt acggcgatat aacgattgat catatttcgg ccgacgggaa
     7621 gattaccagg gtaggcatgg ccaacggggt ggcggtttac acgcccaatg ccacaaggag
     7681 attcggaatc agtcttatgg atgtccccgg agttgattac ggggcgggta aattaagagt
     7741 aacgtattca gcctcatcgg atgtaaaacc agcaaaattt gcggagaatg agctcattct
     7801 gaataaatga tgtacttagc gctttttaat acggacatga tcctgatggt cctggctgtt
     7861 atagcggtac tttcgggatt ttgtctttat tgcccgagcc ggtttatatc ttttatgaac
     7921 aaattttccg gcaataagca ggccagtgcc gcattttttc tgttattcat ttttccggca
     7981 gtatcattgg gtcagacggc aggtgattac cagtctagag gaacaggtaa ttggaatact
     8041 ccgggtacat ggcaaaaata caacgggtca gtgtggcaaa acaatcaggg ctatccgggt
     8101 cagaatcctg gtaccggaac ggttaccatc cgcgatggac acactgttac attgaatgtt
     8161 tctcctacca atcctgttgg taatcttgtt attggtgaag gaagcagcgg aacgcttcaa
     8221 actgacaatt caaacagaat cctaaacact tcgggaaacc tctcagtttc agatggtgca
     8281 acatttaatc tgggaaggat tacattgaca gtaagcggaa caacgagtat caatggatcc
     8341 cttacagata gccacaacaa tggatcggcc agttttaacg ggcagttcat tattaacagc
     8401 aatggttctt tttcaaccac gaacaattcc ccgtttactt tcagcggagg aatcacaaat
     8461 aatggtactt tcagcaaagg tggcagcggc actgccacct ttgccggttc acaggcaata
     8521 aacggttccg gccccttgtc attcagcggc ggcaatgttt caatttccag cggttcatct
     8581 gtgactaatt ccggtattgt cacaatagca ggtgttttga atggcgatga tgccggttca
     8641 caatggatca atggtgccaa tgcaaccctt aattacggta acggctcagt accaatgtcc
     8701 accggcagcc ttgttgccac agccacaaat aatacagtta attacaacgg aacagtgaac
     8761 cagtcagtcg catcaattat ttacagcaat ctgataattt caggctcagg cacctcaact
     8821 ctgactggtg aggtaacggt tgaaaacacc attacactgg catcgggaac tctgtcaata
     8881 tcagcgaata cccttatcct taacggatct cccattgccg gaagtggcag tctcaataca
     8941 acaacaacat caagtcttgt tttcggcggg acatcatcgg gtgtttttgt accatcaaat
     9001 gtttccaatt taagaaatct cactataaac aacccgggag gtgtcacaat aaacaacaat
     9061 ctgaccttat cttcggctgg tgaattgaca ctcacatccg gaatccttga tatagggtca
     9121 aatacgctta gtatagccaa tactgctgca acagccatta caaataactc aggggccttt
     9181 gtgaacatta ctaccggggc gttacgaaga acactacccc gcaatctttc aggaacagga
     9241 aatgattact ttttccctgt aggtgagggc ggcgtatata aaggattaaa tcttatagat
     9301 gttaataccg gctcaccgga acctgtgcta agtgtttccg tcagtccggc cggcgccctg
     9361 gatggtgaca atattacgat cggcccggtt gattccaggt actggaccct gataaaagtc
     9421 agcggaaacc tgaccagtgc aagaattgag ttgttcgaat caggtctgtc ctctcagaag
     9481 accattggta tgtcaaattc cgtgtcaggt ccctatgtat caatcggagg caccctgaac
     9541 ccatcatcaa tgacatcatc tgtcgttagt ggtttgagta cctttttttg cataggggat
     9601 aaaataagat cagataaatt ttattcatat cagactggga actggaactc tgtcaccacc
     9661 tggacctcgg atcccagcgg cacactgcag atcggaaatg aggtgcccgg gaacaccgat
     9721 aaggctatta ttctttctga ccgaacggtg accctgacag gcaacataaa cactacagat
     9781 cttgagctga ccattgaacc ggggggtgtt ctcgaccagg ccggctaccg gttcacaaac
     9841 accctggcaa aactcgaagg ccagggcact ctcaggcttt cttccgtcaa tttcccagat
     9901 gccgtcacga acacatttat aactgccgga ggcggaacaa cggaatacta taacagttct
     9961 gactttacaa ttcctttctc cccttcgtcc tataataacc tgataataaa ctgtaccggg
    10021 cgggttgcaa cattgctcgg cgatctgaca ctttacggga acctggatgt taaagctggt
    10081 aacttcagga taaacgacaa tatctcgaca acgaaactca ttctcacgat caacggcaat
    10141 gtaacggtta acaacgcggc atctgttacc gtgggaaatg gggctactaa cccggccata
    10201 ggatcggcag gattgggggg aagcgcaccc tttctgaact attaccttaa cttccacacg
    10261 gtgataatca ggggtgattt tacaaataac gggacagtaa gattcaccaa tcttacatat
    10321 cctctttata ctgcctttcc acctgcctcg gcaggtccga cctctggcgc tgcatccgtg
    10381 tactttcagg gagcagccga caacacactc acatgcaacg gcacgaccga cttttacaat
    10441 ctcattgtaa ataaaggaac ggatcagaca tacaagctga caataaactc ctccgaatat
    10501 gataatttcc gcttgttcgg cgccaattcc cttgcaacag aatcggtttc agaaaatccg
    10561 cttctgaaga aagcgctctg gattcacacg ggaacgcttg tattgaaagg taagctgatt
    10621 ataccatcac ttacagaagg gacggcaccg aacgctcatt attatattcc ttcaaacggt
    10681 gcactggtga ctgatggcat cgatgttgtc atattcacca ccgctgatga ttacagggag
    10741 gttaacacgt cttatggggt agctgcaccc aacaattcaa cgattggaat aacaaccggg
    10801 ggaaacagtt ctctttacgt gttcgggaag cttcaaataa acaacggaat cctgtcgaca
    10861 cgtgaatcgg gcggtattgt tacttcttca acagcctcgg ggcagataat cataaacgga
    10921 ggcacagttg atgccaaaca aatgctcgga tcaacgggca gcgcttcata tacccagacg
    10981 ggcggactgt ttatcctgag gggccggctt cagaggatcc cggtatctta tgtaacaatt
    11041 gcggatatca ctgatatctc aaatgcaagt ttgggtactt ccaggatcaa caacggaata
    11101 agcactgctc acggttcctt caacctcgaa aatccggcaa atatctattc tgtttccgga
    11161 ggtattatcc ggatttatga tgtaactctc aacactggcg gaaaagcttt cgacgtcaaa
    11221 agctccctgt ccaatatcaa tgttacggga ggaagtcttg aaatcatgcc ggtagcaggg
    11281 acggtattgg cagatgcaac atcattcctg atctacagca atgcccctat ctatgatctt
    11341 tcggtaaaca gactgggcag cacatctgtt gcaggattgt caacacctct gaacgtcctt
    11401 aatgatttaa acctgatctc cggtagttta actgccaata atttcaatct gggcgttggc
    11461 ggtgatttca ccattgaaaa cggggcagca tatagcccgg gtacaaacac caccatcttc
    11521 aacggttccg gaaaccaggt attcacgata aacacaggag cggctttatc actaaatgca
    11581 cttaccctga acaagcaggc aggagacagc atcatattgg caggatcgca gaatataatc
    11641 aatgcagcgg gcagttttaa cctgaccctg ggaacattag ttgacggagg gaagacattg
    11701 aacctctcgg gcggaacaat aatcaattcg ggcatacata caggaacagg tataattaac
    11761 ctgaacggaa cagccgctca gtcaattgat ggcaacggta cgttccagaa tatcagtctg
    11821 aacaatacat caggtacacc ggtttcactt gcggccaata tgaccatcaa cggaaccctg
    11881 actttttcaa acaatgccct gttcaatatc ggcacttaca acctccgtct caatgcttcg
    11941 gcaacgatcg tgaactacgg ggccaaccgg tacattctca ctgcgggaaa tgcaggggac
    12001 ggaggcctca ccagagttta ttcatcgaca gatgcgctgg tttttccggt gggggctccg
    12061 acaataacgc cggcgagggc ggcaaaatac actcccgcaa ccctgggttt tacgtccgcc
    12121 ccgtcaactt acggatcggt gactgttatc cccgtgggtt acgagaatcc caatacaact
    12181 attaaaggac aaagccttac atattactgg cggataaaat catccggatt ttcaggcatt
    12241 ccgctttatt cggtgacaca tacatttgtc tatggtcagc cggatgttgt cggatctgaa
    12301 gccgcctatg ctccagcatt atatgacgaa acggcacatc tctggtatta cggcacaact
    12361 tcatcagtaa acacaaccac caatatcatt tcagactgga caaccccgac caacagcacc
    12421 aacttccttg acgggaatta tacggccggc gttccatcgg gtttcggaac gcctaggatt
    12481 tattacagcc gcagaagcgg cccttgggca aatgtctcaa cctggtcatt gaccggtcat
    12541 accgttgaca atccccctgc tgccgtgccg gggcctaatg acatagtgat gattggcgac
    12601 agggattccg tttacctggc aacacacaac actaatccaa acaccggtgt tcagaattgc
    12661 gccacgcttt tgattgaggc gggctcagcc ctcgacattg gctacaaccc agcctgcagg
    12721 tttgccatgg tactgtcaca tccttccggc aacggaaatt tcaggctaac gacaaataat
    12781 aacactggcg ccatatttgc ctttccttca ggcgatttca gcgatttcaa cgcgaacagg
    12841 gggacaaccg agctttatac aaccaatcca acggcaggaa caacatatta catgcagacg
    12901 ggaatttcaa gctacgggaa cctgatatta tccccccttg gcggatcaaa cctgattttt
    12961 ccgaacaatg atgtgacaat ttacggaaat cttattacaa ggggtcagaa cgcggattca
    13021 tggtttgtac cggcctggca aactacctat cccgggaatg tccaggtgat tcccaaaaca
    13081 atcacgataa tgggtaacat ggatattcag ggcggagcgc ttatctggta tggaagcggg
    13141 aaccgggccc aggatttcgt tatccacggt gatgttaaaa taggggtata cgcctccctt
    13201 tatgtataca gcggtgccac aaaccagagt atggccattg gcgggaattt gatcaatgat
    13261 gcaaacgggc agataaacgg cagcggagtt acaacagtgt ctaaagctga ttttaacaac
    13321 atccccctca ctttcttcgg tcccaattca tcggcggtga caaacaccac gggaatgcct
    13381 tcgacaactt tcggttcagt aacggtaaat aaaggtagtt cacaggaaac aacacttacc
    13441 atcaatatag caggaaccct gaacacacca gccaataact ggcttaactt actgaacggc
    13501 acactgatct acaacagggt cggcaatctc acgatatcgc agggcactga ttttactatc
    13561 ccggcaacct cgggcctggt tataaatact ccttcaaacg tgtatatagc gaacaataac
    13621 gcgattaacc ggacgctttt ccttgacggc cggcttgcca ttaccggtgg cgacggtaac
    13681 gtttacattg gtccagcagg gaacagtacc aataatgcag acattgaata ttcggggagc
    13741 ggtgcttcgc agcttgacat ccaggcgggc aacctctatg tgaatggcca gatcaggagg
    13801 cctctcgcct caaccaatgg tattttaaac tattcacaaa caggaggcaa tgtttatata
    13861 ttcggaaaca atccaacgac tacaaatctt acaaaagcca aacttgaagt tttaaatgaa
    13921 ggcagctcat tcacaatgac agggggtatt cttaccattg taagaggagg agggacgacc
    13981 tttggtgatc tttatttaag gccctcctca tcgtcagtga caggaggtac aattatattt
    14041 acccaggtac catcttcagg acccgtgatc gatgcgcccc agtcctacag ccttgatgct
    14101 aatattcctc tgaacaatct gacaatcacc gggaaaacag caccgaccag aagggctgct
    14161 accctggctc ttatggtaag ttcgctgtca ttgaatggtt ctctaactct tacaaacagt
    14221 tacagtatat tcaattcaaa cgacctgaat atatccctga agggtgatct gaacaacaac
    14281 ggaacatata attacggcac gaatctgact acatttaatg ggggggccca gtttataacg
    14341 ggaagttcaa catcagattt tcacgatctt gatgtactgt cactcacaac ccttacggtc
    14401 agcaacagtt tcacggttgg aagaaatctg aggataagca acggcgattt ggtccttggt
    14461 tcaaacaggg ttacggtgat tggaaatctt gtaaataacg gttcctacac cgacaacaac
    14521 agttccggcg ggattactct tgccggcaca acccggcagc agatttccgg cacgggactg
    14581 ttcgggctcc ttgatatcaa caacacggaa ggtgcaaggt tatacagcga catcaacctg
    14641 aggaacaatc ttgtattgtc caacggagtt ctggatatca acatgtatct tcttaccctg
    14701 agtcagaaca gtataatatc gggtgctccc ttcagtgatc ataaaatgat catgccggat
    14761 ggagtcgtca gcagcccgg
//
LOCUS       DAOOYG010000063        14735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4102_length_14735_cov_6.445845, whole genome shotgun sequence.
ACCESSION   DAOOYG010000063 DAOOYG010000000
VERSION     DAOOYG010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14735
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4102_length_14735_cov_6.445845"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2236
                     /locus_tag="PK288_06865"
     CDS             <1..2236
                     /locus_tag="PK288_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM18680.1"
                     /translation="GNVGVSGSRSIKPDQYLDNPYARLYDHEAEVFPGSNFFTNYISV
                     GSNLGKTNFLISFENNRQTGVVAETDGYKRNSFRLNVDHRLSPKLTLSASNLYVKSTT
                     QDPGGANYYNGGVFFNTLLIEPDVDFKGVNPDGQPYLFIPNIWQDEQENPIYNLWKIE
                     DFTKRDRILGTYSAKYNLSHSINLEGKYAFEYSLSDLTSYSPYDTWSRGGEGPVYSQG
                     EYYRSSRADFSQNFQATLNFNRKFGEFNTRAKLSYLIEKETYKSHTATGYDFSLGGVP
                     SFDAIAGNKSLTSYQDEIIAKNYFAILYLDYKDKYIFDGMYRYDGSSLFGSEARWNPY
                     YRVSAAWRITEDIHIPDVQELKLRVAYGTAGQRPAFADQYEVMAISGGLTSKSQLGNK
                     NLKPSLSKEWEVGLNLDFFNRFSFELIRSETNTTDQILAVPLPVQYGGWPSQVQNAGS
                     LNSKVWEASLNAAVVRQTNLSYNVGFIFDRIRTKITKLTVPPFQTGPQGQEADKCFYI
                     REGEVFGAMYGYKFVRSLDELAQQLGETDNISNYVVNSDGYVILKGTEGTTSEKAIKK
                     LDEEGALWYGKTGDSNPKFKLAMTNNLSYKGFGLYALLEWKNGGDIYNKSAQWLTRDD
                     RHGMMDQYGKPDYLKKTLTYYKTFYDVNDFNDFWVEDGSYLKLREISLSYTVRSAALN
                     NIFNGFVKGVKFSILGKNIWTLTNYSGYDPEVATTDGTQYFSYDFMGYPNYRSFAASL
                     ELKF"
     gene            2256..3752
                     /locus_tag="PK288_06870"
     CDS             2256..3752
                     /locus_tag="PK288_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18681.1"
                     /translation="MKTYKYLFLLTVLTGLILTGCESLDVKNLNDPDFATAFSKPSDV
                     KGVAGGLINTWFQYTQEYNGPALALWVGADAGTCSHGNAAMWHFSMEPRIAWDNTPSY
                     SYAVITENFYKGMYSLLSSSNEILEKVVGENMKITADDGSDETPMVKAMAYLGQGLAL
                     GYVGLFFDKGFVVTDETDLGETIPVSSYTELITAAIASLDKCIAVCESSTFTLPGPWI
                     PGKTYTQVEIGQLANSMAARLLSYSPRNKAENDAVDWGKVLAYANKGLTYDFAPKMDD
                     INWYDLYHTYANYAGWGRTDMRIAHMMDPAMPERWTLGADQWAALPPPTTAHKDGVDD
                     RIFTDFQYLSSCTFRVERGYYHYSCYRYSRLDDYLATWITPSPVFRKAENDLLKAEAM
                     LHQNNYSGAADIINAGERVTRGSLPPIGATAAEVDAAIFHERNIELYCSGLGVEFCTM
                     RKADKLQKGTPLHFPIPGQQLEVNLMESYSFGATKGVAGKDYSNGGWF"
     gene            3788..4597
                     /locus_tag="PK288_06875"
     CDS             3788..4597
                     /locus_tag="PK288_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18682.1"
                     /translation="MNKKILIRLTSLLVAVSLLAACEKTPMEKAQDAYDASQVVPVVL
                     STTGPSLVLQTFSYDYSVDYFRAGSTWSWSAVDATVSSVSQDTRTAKVLFDKLPASGK
                     AQIKVVETTAGGVKSPEKVIEVNVNPFCPLPVEDFAGTWQGTDGAADETYPSTVTAVL
                     EGDKVVLTGLNEGFMDGFWGESIIAGGSCKVTVNPDGTLVIPEQYFCETDYIDGYKIK
                     GSGTWDNCGAKPTMKIDYDIWFPEDGIWIAAKYGPSYLGGKTILTANLTKE"
     gene            complement(4700..5458)
                     /locus_tag="PK288_06880"
     CDS             complement(4700..5458)
                     /locus_tag="PK288_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPM18683.1"
                     /translation="MEKYICENVFVPLRSAPSHKSEMLSQVLFGEKYSIIDSAGHWYK
                     IKTCFDDYSGWIDMDHLCHSPDTDGSGGNVITRSLSCFRPDGTRLVLEPGCEIYHPDF
                     ERKTFMLSRKTYTAGEEFTESIIRNDLSLSDTAMRFLNSPYIWGGRIPSGIDCSGFTQ
                     LVYKLLGKAIPRDSRQQAEKGEIISFLEEAKAGDLAFFDNERGVITHVGLILAKGLII
                     HSSGRVKIDIIDQQGIFRQGKYSHHLRLIKRMQS"
     gene            5607..7718
                     /locus_tag="PK288_06885"
     CDS             5607..7718
                     /locus_tag="PK288_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPM18684.1"
                     /translation="MAESRRPPEIKPDRKYLFHLAFFIASAIIVVLISPREGKFRYEF
                     QKGKPWLNSSLIAPWDFPVLKPASVIARERDSISRNFTPYFRTSPEISSLELEKFKEY
                     LDASLNDYLAVHKQVPASLKNKLHEDLLEILSEIYRRGILETDAIGSSYGQNFRDVTV
                     INGKIAEKKPFGSFYLQKTAYLAAEQKKEELMNGFKAAADKPVRDFLENVAFYNFIRP
                     NLIFDPQTSSAVRDKMLSEVSPNRGMIQEGELIISRGEIVNDAKYLILESLRDESERR
                     LGMYDKWLVVLGRIILVCSCYMALYLFMYHFRFDVLSSTQKTFFIILIILLFIAIIRL
                     MLYAPSDVVFLVPLPIIPIVIRTFYDSRLALFVYLIAIMLAGFIVPNSFEFVFISYLA
                     GVIVIFSLTNINRRAKLFFTAFMVFLAYSVVYFGIGIMQEGSLENMNWRNYIWFAGNG
                     VLLLLSYPLIFVFEKTFRFLSDATLFELADTNQPLLRKLSENAPGSFQHSLQVANLAE
                     EAAREIGANNLLARTGALYHDIGKIVNAQYFIENQSEGFSPHDRLDPEKSARIIIDHV
                     KEGVELARKHKLPSQIIDFIQTHHGTSVAYYFYKKFLDKNPDRTDMQKNFSYSGPKPF
                     SRETAIVMMADSVEASSRTLPEYTEDKIRDLVERIIYLQEQDGLFSETPLTFRDVTDI
                     KRVFVKRLLNIYHARIAYPAR"
     gene            complement(7729..8367)
                     /locus_tag="PK288_06890"
     CDS             complement(7729..8367)
                     /locus_tag="PK288_06890"
                     /inference="COORDINATES: protein motif:HMM:NF027898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPM18685.1"
                     /translation="MKKILFLAFLQLLFPARGFSQNDPAALAVLDRFSSRALGAPSVS
                     MEFNLRTENLADRTSSSRKGSVILAGDKYKLDLEDNIIWFNGTTSWNLLPAEKEVTIT
                     TPGKNDDSFLGKPSQIFSLYRKGYKSRLLEEKADSYLIDLYPEELNSDHVRIRLKIGK
                     PALDLRNLEYKYKNGTTITLDVTSFDLKKNPSSTTFIFEPEKYRGVEIIDMR"
     gene            complement(8378..10816)
                     /locus_tag="PK288_06895"
     CDS             complement(8378..10816)
                     /locus_tag="PK288_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPM18686.1"
                     /translation="MKKEKEEAARRKSKKGGKTSTFFKDERIKFILGILITGFAFYLL
                     LACISYIISWKTDLSLPDSEVFSEPGVNVRNWSGKGGHFLAKMIIGFGFGFGAFFIPL
                     IIGVTGLYLLNFPKINLLKLVTKLTLATILLSLILGFIFGDANGYLYGGPGGEQGYKV
                     TQWLNSFAGKTGTAILLLILTIAYLVFALRFKPETFTDKIPATIKGVVPPISFGKTSD
                     KIIDDGEGSGGPDGSDEEGDAGEFYGAADHDNSTFAINRPGSEDYEQEAGLKKQIIHE
                     IGRNEVTGREPGPAHTEINRPGITGIMTEEEVRQIMEDYDPRLDLSRYKFPPVSILKE
                     YRSTADSDDNEVLENKEIITRTLADHKISIRHKAATVGPTVTLYEIVPESGVKISRIK
                     ALENDIALNLSALGIRIIAPIPGRGTVGIEVPNKNPKIVSMRSLITSRVFQETKYDLA
                     LALGRTITNEPFIVDLTKMPHLLVAGATGQGKSVGINAIITSILYKKHPAEVKFVLID
                     PKRVELPLYAKIERHFLAKLPGDEDAIITDTQKVINTLKSLTFEMDNRLELLKEAQSR
                     NIKEYNDKFISRRLNPEKGHRYLPYIVLIIDEFADLIMTAGREIEPPIGRLAQLARAV
                     GVHLIISTQRPSTNIITGFIKANFPTRIAFRVFSSIDSRTIIDATGADRLVGRGDMLV
                     SSGAESVRVQCAYIDTSEIEELTEFIGSQQGYPEAMHLPEYIDDSETGQTEVDLRKRD
                     SMFDDAARLVVQHQQGSTSLIQRKLSIGYNRAGRIIDQLEAAGIVGPFEGSRAREVLV
                     SDFMHLEQILKGLE"
     gene            complement(11033..11743)
                     /locus_tag="PK288_06900"
     CDS             complement(11033..11743)
                     /locus_tag="PK288_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18687.1"
                     /translation="MLNSSQSVIRAGYIHLFIFFAISLNISCKKNGSGNLDQGEIHYH
                     VEYPENSTILPPELMPKTMVVSFKDNNLLFELISPFGNSGITNLSNPSKKIYDTYFSL
                     FTIKCFYPAKPGETYPGFESMEGMQITKTPKTAIICGMHCKNAEVTVPSVPGRKYNIW
                     YTNEIPVSNPNASTPYAEIDGVLMSFFYLMGKSEFHFEAVNIYRKKIPDEAFDRRSRF
                     NEVSRTDLIKFISKMVSL"
     gene            12032..13033
                     /gene="argF"
                     /locus_tag="PK288_06905"
     CDS             12032..13033
                     /gene="argF"
                     /locus_tag="PK288_06905"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPM18688.1"
                     /translation="MTISFRNRSFLKLLDFETEEIQYLLDLAASLKKAKRSGTEQQYL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGANVTYLGPTGSQIGQKESMRDTARVLGRMF
                     DGIEYRGFGQEIVEELSAYAGVPVWNGLTNEFHPTQVLADLMTMAEHCNKSLDSISFC
                     YLGDARYNMGNSLLVGASKMGMDFRTAAPPEFQQGSNLVDRCRIIAGQTGARILITDD
                     LDEAVKGVDFLYTDVWVSMGEPDSVWEERIRLLRPYQVNSAVIEKTGNPDVKFLHCLP
                     AFHNRETKVGEEIFRKFGLDGMEVTEEVFESENSVVFDQAENRLHTIKAVMVATLTD"
     gene            complement(13074..13553)
                     /locus_tag="PK288_06910"
     CDS             complement(13074..13553)
                     /locus_tag="PK288_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPM18689.1"
                     /translation="MSDYILLILAIILMILGIIGCLVPILPGPPLSYLGMILLHFSRF
                     AHFSSNVLIIMAVIAALVTALDFIVPVWGTKKFGGSKYGIRGATVGLIIGLFLGPPGI
                     IIGPFIGAFVGELIFKDDIRYALKAGFGSLLGFLTGIGLKLAASFIMTFYFVKELIT"
     gene            complement(13554..13970)
                     /locus_tag="PK288_06915"
     CDS             complement(13554..13970)
                     /locus_tag="PK288_06915"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPM18690.1"
                     /translation="MEEEAGIKEATVGTEEKANKEEIFTRVVRAGKRTYFFDVKATRK
                     DDYYLTITESKKRLGKEGKVFYEKHKIFLYKEDFEKFTDGLKDAVSFIDNGYGELKQL
                     ESQPGNDTKQESKNISTEEFTNIDFDDLGKNKEVSF"
     gene            14133..>14735
                     /locus_tag="PK288_06920"
     CDS             14133..>14735
                     /locus_tag="PK288_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor"
                     /protein_id="HPM18691.1"
                     /translation="MISRRLLRIKAMMALYAFNRREDGNLEQAEKELMFSIDKTYDLY
                     HLLLLLILEVADVAAGKIDQALQKHIPTHEDLNPKRKFIDNLVIAQLRKNKPFNDYIS
                     SRKLSWANYQHIPRLMYASMIAWKKYDSYMSSEEYGYSGDRKFIISLVTELFAASEDL
                     EANLEEQSIYWNDDTEYVVLMIEKTLKKFKPDSGNDTKLMP"
BASE COUNT         3972 a         3300 c         3392 g         4071 t
ORIGIN      
        1 tggcaacgtg ggagtctcgg gatcaagatc aataaagccg gatcagtatc ttgacaatcc
       61 ttatgctcgt ttatatgatc atgaagcaga ggtatttccc ggaagcaatt tttttaccaa
      121 ctatatatca gtcggttcta acctgggaaa gacgaacttc ctgatctctt tcgagaataa
      181 ccgccagact ggtgttgttg ccgagacgga cggctacaag cgtaacagtt tcaggctcaa
      241 tgtcgaccac aggctgtcgc caaaacttac tctctccgcc tcgaacctgt atgtgaaatc
      301 cacgactcag gaccccggtg gtgccaacta ttacaacgga ggagtgttct ttaacaccct
      361 gcttattgaa cccgatgttg acttcaaggg agtaaatccc gacggacagc cttacctgtt
      421 tataccaaac atctggcagg atgagcagga aaatccgatc tacaatctct ggaaaattga
      481 ggattttaca aagcgtgaca ggatcctggg tacttattcg gcaaaataca acctttctca
      541 ttcgattaat ctcgaaggaa aatatgcctt tgaatattcg cttagcgatc tgaccagtta
      601 cagtccttac gatacctggt cgcggggcgg cgaaggcccg gtctattcac agggagaata
      661 ctacaggtca agcagggccg acttttccca gaatttccag gctaccctta atttcaaccg
      721 gaagttcggg gagttcaata ccagggccaa actgagttat ctcattgaaa aggaaacata
      781 caaaagtcac actgcaacgg gttatgactt cagcctcggc ggagttccct ccttcgatgc
      841 cattgcagga aacaaatctc ttaccagcta ccaggatgaa attattgcaa agaactattt
      901 cgccatcctt tacctcgatt ataaagacaa gtatattttc gacggaatgt accgctacga
      961 cggttcttcc cttttcggct cggaagcaag atggaatccg tattacaggg tttcggctgc
     1021 atggaggata accgaggata ttcatatacc ggatgtacag gaactcaagc tcagggtcgc
     1081 ttacggtact gcaggacaaa gacctgcttt tgccgatcag tatgaagtga tggccatcag
     1141 cgggggattg acctcaaaat cacagcttgg caacaagaat cttaaacctt ctctttccaa
     1201 ggaatgggag gtcggtctga atctcgattt cttcaacagg ttcagttttg agctcatcag
     1261 gtctgaaacc aataccaccg accagatcct tgcggttccg cttccggtgc agtacggtgg
     1321 atggcccagc caggttcaga acgccggttc ccttaattca aaggtatggg aagcatcatt
     1381 gaatgcagct gttgtaagac agaccaatct cagctataat gtcggattta tttttgacag
     1441 gatcaggacc aagatcacaa aactgaccgt tcccccgttc cagaccggtc ctcagggtca
     1501 ggaagccgat aagtgcttct atatccgcga aggtgaagtt ttcggagcaa tgtacggtta
     1561 caaattcgtc agatcactcg atgagcttgc ccagcagctt ggcgaaacag acaatatcag
     1621 caattacgta gtcaacagtg atgggtatgt gatcctcaaa ggtacggaag gaacaacatc
     1681 cgaaaaagca atcaagaagc ttgatgagga aggagcttta tggtatggca aaactggcga
     1741 ttcaaatccg aagttcaaac tggcaatgac caacaatcta agttacaagg gcttcgggct
     1801 ttatgccctc cttgaatgga agaacggagg tgatatctat aataaatcgg cgcagtggct
     1861 caccagggat gacaggcatg gtatgatgga ccagtacggc aaacccgact acctgaagaa
     1921 aacacttaca tattacaaga ctttctatga tgtcaacgac ttcaacgatt tctgggtcga
     1981 ggacggaagc taccttaaac tcagggaaat atccctgtca tatactgtca ggtcagcagc
     2041 tcttaacaat atattcaacg gatttgttaa gggagtcaaa ttttctattc ttggcaagaa
     2101 tatctggacc ctgacaaatt attcaggtta tgatccggag gttgctacaa ccgacggcac
     2161 gcagtatttc tcctacgatt tcatgggata cccgaactac aggtcgttcg ccgcatctct
     2221 tgaactgaag ttctaattat taaactatta tggttatgaa aacatacaaa tatctattcc
     2281 tcttaacggt tcttactgga ttaattctga caggttgcga aagtctggat gtcaagaacc
     2341 tgaacgaccc ggattttgcc acggcatttt ccaaaccttc tgatgtgaag ggagttgcag
     2401 gaggtctcat aaatacctgg ttccaatata cccaggagta taatggaccg gcgcttgccc
     2461 tttgggtcgg tgctgatgcc ggtacctgct cccatggtaa tgccgcaatg tggcatttca
     2521 gcatggaacc cagaattgca tgggataata ccccgtctta cagttatgcc gtgattacag
     2581 aaaacttcta caagggcatg tattcccttc tctcctcatc aaatgaaatc cttgaaaagg
     2641 ttgtgggtga gaatatgaag atcactgccg atgacggaag cgatgaaact cccatggtta
     2701 aggcaatggc ataccttggt cagggacttg cattgggtta cgtgggcctg tttttcgaca
     2761 agggattcgt cgtaacggat gaaaccgacc ttggagagac tattccggta tcctcttaca
     2821 ctgaactgat caccgcagct attgcaagcc tggataaatg cattgccgtc tgtgaaagca
     2881 gcacgtttac actcccaggc ccctggatac ccggtaagac ctatacacag gtggagatag
     2941 gccagctggc caattctatg gctgccaggc tgctttccta ttcaccgagg aacaaggcag
     3001 aaaatgatgc ggttgactgg ggcaaggtac tcgcctatgc caacaagggt ctgacctacg
     3061 attttgcacc caagatggat gatatcaact ggtatgattt atatcacact tatgcaaatt
     3121 atgccggatg gggcagaacg gacatgagga tcgcccatat gatggaccct gcaatgcctg
     3181 agcgctggac actcggggcc gatcagtggg cagcccttcc tcctccgacc acggctcata
     3241 aggatggtgt tgatgaccgc atttttaccg atttccagta tctgtcatcc tgtactttca
     3301 gggtggagag gggttactat cactattcct gctacagata cagcaggctg gatgattacc
     3361 ttgccacatg gatcactccc tcacctgttt tcagaaaagc cgagaacgac ctgctgaaag
     3421 ccgaagcaat gttgcatcaa aacaactaca gcggtgcagc cgatataatc aacgccggtg
     3481 aaagagtaac cagaggcagc cttccgccaa taggtgcaac tgccgccgag gtggatgccg
     3541 caatcttcca tgaacgcaat atcgaacttt attgcagcgg tcttggagtt gaattctgca
     3601 caatgcgcaa ggcagacaag cttcagaagg gtactcccct tcatttcccg atccctggcc
     3661 agcagctcga ggtcaatctg atggaatcct acagtttcgg tgcaaccaag ggtgttgcag
     3721 gcaaggatta ttcaaatggc ggatggtttt agccccactt aataccttaa ttattaaata
     3781 acagattatg aataaaaaaa tattaatcag gttaacatca cttctggtgg ctgtttcact
     3841 cctggccgcc tgtgaaaaaa ccccgatgga gaaagcacag gatgcttatg atgcatccca
     3901 ggtcgttccc gtcgtcctca gcaccacagg tccgtcactt gtgcttcaga ccttcagcta
     3961 tgattactct gtcgactatt tccgggcagg atcaacctgg agctggagtg cagttgatgc
     4021 tactgtttct tcggttagcc aggatacaag aaccgcaaaa gtactgttcg ataagcttcc
     4081 ggccagcgga aaggcccaga ttaaagtggt tgagacaact gccggcgggg ttaaatcgcc
     4141 tgagaaagtg atcgaagtaa atgtgaatcc cttttgcccc cttcctgtgg aagattttgc
     4201 cggaacatgg caaggaacgg acggggcggc tgatgaaacc tatccaagta cggttactgc
     4261 cgtcctggaa ggagataaag ttgtccttac aggactcaat gaaggtttta tggatggttt
     4321 ctggggagag agcattattg ccggcggcag ctgcaaggtt accgtcaatc ctgacggaac
     4381 gctggtcatc cccgagcagt atttctgcga aaccgactat atcgacggat acaagatcaa
     4441 gggaagcggt acatgggata attgcggggc caaaccaaca atgaaaattg attatgatat
     4501 ctggttccct gaagatggca tctggatagc agccaaatac ggacccagtt acctcggggg
     4561 caaaaccatc ctgacagcaa acctcacaaa ggaataaatt atcaaataat taaagctgag
     4621 ggtgtttcaa cggaaacacc cttttttatt caatccgggt aaattgctta acatgcctca
     4681 gattaccata tgttgttttc tatgattgca ttctttttat cagcctgagg tgatgtgagt
     4741 atttaccctg cctgaatatg ccctgctggt cgataatgtc aattttcacc ctgcccgatg
     4801 aatgaataat gagtcctttg gccagtataa gtcccacatg ggttatcacc cctctttcgt
     4861 tgtcaaaaaa ggccagatcg cctgcctttg cttcttcaag aaaactgatg atttctccct
     4921 tttctgcctg ctgcctgctg tcgcgcggaa ttgccttgcc cagaagctta taaaccagct
     4981 gggtgaaccc tgaacaatca attcctgaag gtatccgtcc accccagatg tacgggctgt
     5041 taaggaatct cattgcagta tcggataaag acagatcatt tctgatgatg ctttcggtga
     5101 attcttcacc ggcagtatag gttttcctcg atagcatgaa agtcttccgt tcaaaatcag
     5161 gatgatatat ctcacaaccc ggctcaagga caagcctggt cccgtcaggc cggaaacagg
     5221 acagtgacct ggtgatgaca ttgcccccgg aaccgtcagt atcaggtgaa tggcacaggt
     5281 gatccatatc gatccagccg gaatagtcgt cgaagcaggt ttttatttta taccagtgac
     5341 ctgcactgtc gataatcgaa tatttctctc caaagagaac ctggctgagc atttctgatt
     5401 tatgtgaggg agccgaacgc aaaggaacaa aaacgttctc gcagatatat ttttccattg
     5461 gttttgattt ttctcaaaat taacaaatta atgatttccc gcgtgaagcc ggctgaccgg
     5521 atttaaatgg ttccggctga tgagggataa ataaaaaaag catatttttg ccaatgattg
     5581 tcggttaagt ctttaatgag cgtgtaatgg ctgaatcaag gaggcctccc gaaattaaac
     5641 cggacaggaa atatctcttt cacctggctt tttttattgc aagtgcaatt attgtggtgc
     5701 tcatctcccc gcgcgaaggc aaattcaggt atgaattcca aaagggaaaa ccatggctga
     5761 attcatcact cattgctccc tgggatttcc cggtgctgaa accagcctca gtaattgcaa
     5821 gggagcgtga cagcatttcc aggaacttca ccccatattt caggaccagt ccggaaatat
     5881 catcactgga gctggaaaaa tttaaggaat atcttgatgc ttctttgaat gattacctgg
     5941 cagtacacaa acaagtgccg gcatcgttga aaaacaaact ccatgaagat ctgctggaaa
     6001 tactgtcgga gatctaccgc aggggaattc ttgaaacaga tgcaatagga tcctcctacg
     6061 gtcagaattt cagggatgtt accgtaatca acggtaaaat tgctgaaaaa aaaccatttg
     6121 gttcctttta tcttcaaaaa acagcatacc tcgcggctga acaaaaaaag gaagaactga
     6181 tgaacgggtt caaagcggca gccgataaac cggttcgtga ttttcttgaa aatgttgcat
     6241 tttacaactt tatcaggccg aacctgatct ttgatccaca gacttcttca gcagtcaggg
     6301 ataagatgct gagtgaggta agcccgaacc ggggaatgat ccaggaaggg gaacttataa
     6361 tcagccgagg tgagatagtg aatgatgcaa aatacctgat tcttgaatcg ctcagggatg
     6421 aatcggaaag acgcctgggg atgtacgaca aatggctcgt ggtactggga agaattatcc
     6481 tggtatgttc atgttatatg gcgctttacc ttttcatgta ccatttcagg tttgatgtcc
     6541 tgagttcaac ccagaagaca tttttcataa tccttatcat ccttcttttc attgccatta
     6601 tcaggctcat gctctatgcc ccttccgacg tggtttttct tgtacccctg ccaatcatac
     6661 ccattgtgat caggactttc tatgattcaa ggctcgccct ttttgtttac ctgattgcaa
     6721 tcatgcttgc tggcttcatt gtaccaaact cgtttgaatt cgtttttata agctacctgg
     6781 ccggggtaat tgtgatcttt tcactgacca atatcaacag gagggcaaag cttttcttta
     6841 ccgctttcat ggttttcctt gcctactcgg tggtttactt tggtattggc ataatgcagg
     6901 aaggcagtct tgagaacatg aattggagaa attacatatg gtttgcggga aacggggttc
     6961 tccttctgct ttcttatccg ctgatctttg tttttgaaaa gactttcaga ttcctttccg
     7021 atgcaacact gtttgaactg gcagatacta accagcccct tctgcgaaag ctttccgaaa
     7081 atgccccggg ttctttccag cattcccttc aggtggcaaa tcttgcagag gaggccgcca
     7141 gggaaatagg cgctaacaat ctcctggcca ggacaggtgc cctttatcat gacatcggga
     7201 aaattgtcaa tgcacaatac tttatcgaaa accagtcgga aggattcagc ccccacgaca
     7261 ggctagatcc ggaaaagagc gcaagaatta tcatcgacca tgttaaggag ggtgttgaac
     7321 tcgccaggaa acataaactg ccttctcaga taattgattt cattcagaca catcacggga
     7381 cttcagttgc ctattatttc tataaaaaat tcctggataa aaatcctgac aggaccgaca
     7441 tgcaaaagaa cttctcctac tcagggccca agcctttttc cagggaaaca gcgattgtca
     7501 tgatggctga ttcggtggaa gcatcgtcaa ggactctgcc tgaatacacg gaagacaaga
     7561 tcagggatct tgtggaaaga atcatttatc tgcaggaaca ggacggactt ttttccgaga
     7621 cacctctcac attcagggat gtgactgata ttaagagggt ttttgtgaaa agactcctga
     7681 atatctatca tgcacggatt gcttatcccg ctagatgagt tttgagcctc atcgcatgtc
     7741 gatgatttcc actcccctgt atttttccgg ttcaaaaatg aatgtagtac tggaggggtt
     7801 cttcttcagg tcgaatgatg tgacatcgag ggttatggtt gtaccgttct tgtatttata
     7861 ttcaaggttt ctgagatcaa gggcgggctt ccctattttc agccgtatcc tcacatgatc
     7921 gctgtttagc tcctccgggt agaggtcgat gagatatgag tctgcctttt cttcaagtaa
     7981 ccttgatttg taacctttcc tgtacaatga aaatatctgt gagggtttgc ccaggaatga
     8041 atcatcattt ttcccgggag tggtgattgt tacttccttt tctgccggca ggaggttcca
     8101 cgaggttgtg ccgttgaacc atatgatatt gtcttcgaga tccagcttgt atttgtcacc
     8161 ggcaaggata acggagcctt ttcgtgagct gctggtcctg tcagcaaggt tttcggtacg
     8221 gaggttgaac tccatcgata cggaaggtgc tccgagggcc ctggaagaaa acctgtcaag
     8281 cactgcaagc gccgcgggat cattctgtga aaatccccgt gcaggaaaga gaagttgaag
     8341 aaaagcaaga aacagaatct ttttcataat atgtgatcta ttccagaccc ttcaaaatct
     8401 gttccaaatg catgaaatcg gaaaccagga cttcccttgc cctgcttccc tcgaatggac
     8461 cgactatacc cgcagcttca agctggtcaa ttatccttcc ggccctgttg taccctattg
     8521 acagctttct ctggataagg gaggttgagc cctgctgatg ctgtacgaca agccttgcag
     8581 catcatcaaa cattgaatca cgcttgcgca gatccacctc agtctgtcct gtttcactgt
     8641 cgtcaatgta ttcaggaagg tgcatggctt caggatatcc ctgctgtgat ccgataaatt
     8701 cggtaagctc ttcgatttca gaggtatcga tataagcaca ctgaaccctc acggattcgg
     8761 cccccgatga aacgagcata tcaccgcgtc ccactagcct gtcggctccg gttgcatcaa
     8821 tgattgtacg tgagtcgatg gatgagaata ccctgaaagc aatcctggtg ggaaaattgg
     8881 cctttataaa accggttatg atgttggtgg agggcctctg ggttgaaatg atcaggtgga
     8941 cccccactgc ccttgcgagc tgtgccagcc ttccgatagg gggttcaatc tcccttccgg
     9001 ccgtcataat cagatctgca aattcatcga ttatgagaac aatataggga agatacctgt
     9061 gccctttttc agggttcagc cttcttgata taaatttatc attgtactcc tttatgttcc
     9121 gcgactgggc ttccttgaga agttccagcc tgttgtccat ctcgaaggtc agtgatttga
     9181 gtgtgtttat caccttctgg gtgtcggtga ttatggcgtc ttcgtcaccg ggaagctttg
     9241 ccaggaaatg ccgttcgatc tttgcataca ggggaagctc aacgcgcttg ggatcgatca
     9301 gcacaaattt aacttcagcc ggatgctttt tatacagaat tgaagtgatt atggcattta
     9361 ttcccactga ttttccctgt ccggttgccc cggcgacaag caggtggggc attttcgtca
     9421 ggtcaactat aaaaggttcg tttgtgatgg tccttccgag tgcgagtgca aggtcatatt
     9481 tcgtttcctg gaacaccctg gatgttataa gggaacgcat ggaaacgatc tttggattct
     9541 tgttgggcac ctcaatacct accgtgcccc tcccggggat cggggctatt attcttattc
     9601 cgagcgccga aaggttaagc gcaatgtcat tttcaagcgc ttttatacgg gatattttaa
     9661 ctccgctttc gggaacaatc tcgtaaaggg tgaccgtggg tccgacggtt gctgccttat
     9721 gcctgatact gatcttgtga tctgcaagcg tcctggtaat aatctccttg ttttccagga
     9781 cttcattgtc atcagaatca gcggtcgatc tgtactcttt aagtattgaa acgggaggaa
     9841 atttataacg tgaaagatcg agccttggat catagtcttc catgatctgc cggacttctt
     9901 cttccgtcat aattccagtg atcccgggcc tgtttatttc agtatgagcc ggccctggtt
     9961 cccttcccgt aacttcattc ctgccaattt catgaattat ttgttttttc aggccagcct
    10021 cctgttcata gtcttcactt cccggcctgt tgatggcaaa tgttgaatta tcatgatccg
    10081 cagctccgta gaattctccg gcatcgcctt cttcatcaga tccgtcaggt cctccggaac
    10141 cttctccgtc atcaatgatc ttatccgatg ttttgccaaa gcttattggc ggaacaacgc
    10201 ctttgattgt tgccggtatc ttatcggtaa atgtttcagg tttgaatctc agggcaaaga
    10261 ccagatatgc aatggtcagg atcaggagca atatcgccgt gcccgtcttt cccgcaaagg
    10321 aattcagcca ttgtgtgact ttatatccct gttctccccc gggcccgccg taaagatacc
    10381 cattggcatc cccgaaaata aaaccaagaa taagcgatag gaggatcgta gccagtgtga
    10441 gtttcgtaac aagttttaag agattgatct tcgggaaatt aagcagatac aatccggtaa
    10501 ccccgatgat cagtggaata aagaaagctc cgaatccgaa tccgaatccg attatcattt
    10561 tggcaagaaa atgcccgcct ttccctgacc agttcctgac gtttacaccg ggttccgaaa
    10621 atacctcgga gtcgggcagg ctcagatcgg ttttccacga tattatatag gaaatgcatg
    10681 caagcagaag ataaaaggcg aagccggtga tcagaatgcc cagaataaat ttaattctct
    10741 catctttgaa gaaagtactg gtttttcccc cttttttgct ttttctcctg gctgcttctt
    10801 ccttttcctt tttcattgcc gtttatgatt tctgtgggga caaagttgtt caaatgcctt
    10861 ggttccccgg aaaagcatca caaaggtaat aattttgaac tatcgcggca ggggttgcaa
    10921 aacaaaatga atgactaaaa ggaacctgca tgacaggctt tccgaaactt cgaaaaaggg
    10981 cggatttttc cagattcagt tacacaatgc cttagtattg agacattcac tatcagaggc
    11041 tcaccatttt gcttatgaac tttatcagat cggttcttga aacctcgttg aagcgggatc
    11101 tcctgtcaaa tgcttcatcg gggattttct tcctgtaaat attcactgcc tcaaaatgga
    11161 attccgattt tcccataaga tagaaaaaac tcataagaac accatctatt tcagcataag
    11221 gtgttgatgc attgggattg ctcaccggga tttcatttgt ataccagata ttgtatttac
    11281 gtccgggaac ggaaggtact gtaacttcgg cgtttttaca atgcatgccg cagataattg
    11341 cagttttcgg ggtcttggtg atctgcatgc cttccatcga ctcaaagccc ggataagttt
    11401 cacccggttt tgcaggatag aaacatttta tggtgaaaag gctgaaatag gtatcgtaga
    11461 ttttcttgga cggattggaa agatttgtta ttcctgaatt gccaaaggga gaaatgagtt
    11521 caaacaggag attgttgtct ttgaaggaaa ccaccatcgt tttgggcata agttcgggcg
    11581 gaaggatggt tgaattctcg ggatattcca catgatagtg gatttcaccc tgatccagat
    11641 taccggatcc gttctttttg caggaaatgt tcaaggatat tgcaaaaaag ataaaaagat
    11701 gaatatatcc ggctctgatc acgctctgac ttgaattaag catctaaatg tagatatttt
    11761 ttttcattcc ggacagcaaa ggtatcataa attaaactta ttactgattt gtgactcaca
    11821 ctgcaactga tcttactaac ctttggcttc cttcttgaat attgatggct gattcagtcc
    11881 ggaaattacg atgcttgcct gaacctcggg aaaattcaat tcaggtttca ttccccggtt
    11941 gcgaatattt caatcatcgt aaaaaaacaa ttacatttga tcccttcaat tgatcagcca
    12001 atcattatat gaatcataaa cacgtaaatt catgacaatt agttttcgta accgcagctt
    12061 ccttaaattg cttgattttg agacggaaga aatacaatat ctcctggatc ttgcggccag
    12121 cctgaaaaaa gccaaaagat cgggtaccga gcagcaatat cttaaaggga agaatatcgc
    12181 gcttattttt gaaaagacat caacgcgtac gcggtgtgcc tttgaggttg cagcttatga
    12241 tcagggggca aatgtaacat atcttgggcc gaccggttcc cagatagggc aaaaggaatc
    12301 tatgcgcgac actgccaggg tactgggaag gatgtttgac ggtattgaat acagggggtt
    12361 cggacaggaa attgtcgaag aactttcggc atatgccggt gtaccggttt ggaacgggct
    12421 gaccaatgaa tttcacccca cgcaggttct ggctgacctg atgacaatgg ccgagcattg
    12481 caataagtct cttgacagca tctctttctg ttatctgggt gatgcgcggt acaatatggg
    12541 taattcgcta ctggtggggg catctaaaat gggaatggac ttcaggactg ccgctccgcc
    12601 tgaatttcag caggggagca accttgttga ccgttgcagg attattgccg gccagacggg
    12661 tgccagaata cttatcactg atgatcttga tgaagctgta aaaggagtgg atttcctcta
    12721 taccgatgtc tgggtatcta tgggcgagcc tgattcagta tgggaagaga ggatcaggct
    12781 tttgaggcca taccaggtga acagcgccgt tattgaaaaa accggaaacc ctgatgtgaa
    12841 attccttcac tgcctgcctg cgtttcataa tcgtgaaaca aaggtaggag aggagatttt
    12901 ccggaaattt ggacttgacg gcatggaggt tactgaggag gtctttgagt cagagaattc
    12961 ggttgtattt gaccaggctg aaaacagact ccatacaatt aaggcagtga tggttgcaac
    13021 attgacggac tgaaagcgaa aaaacagccc tgattacaat gtttcgtctg ccatcaggta
    13081 atcagctcct taacgaaata aaaggtcatt ataaatgatg ctgcaagttt cagtccaatc
    13141 ccggtcagga atccgagaag gcttccgaag ccggctttaa gggcatatct gatgtcatcc
    13201 ttgaagatca gctctcccac gaaggcaccg atgaatggtc ctattattat cccgggggga
    13261 ccgaggaaaa gcccgatgat caatccgact gtagctccgc ggatcccgta ttttgaacct
    13321 ccgaatttct tcgtacccca gaccggtaca atgaagtcca gcgctgttac cagagctgca
    13381 ataacagcca tgatgatgag aacattggac gaaaaatggg caaatctcga aaaatgcagg
    13441 agaatcatac cgaggtagct caggggaggg cccggaagta tcggcacaag acacccgatt
    13501 attccaagga tcatcagaat aattgcaagt attaagagta tatagtcaga cattcagaat
    13561 gatacttctt tgttttttcc aagatcgtca aagtcgatat tggtaaattc ttcggtggag
    13621 atgtttttgg attcctgttt tgtatcattc ccgggctggg attccagttg ttttaattcg
    13681 ccataaccat tatcaataaa gcttacggca tctttgagtc cgtcggtaaa tttttcaaaa
    13741 tcttccttgt ataggaatat tttgtgtttt tcgtaaaaaa ccttgccctc tttgcccagg
    13801 cgttttttgc tttccgtaat ggtcaggtaa taatcatctt tccttgtagc cttgacatca
    13861 aaaaaatagg ttctttttcc tgctcgtaca accctggtaa agatctcttc cttgttggct
    13921 ttttcttcag taccaactgt agcttcttta attccggctt cttcttccat ttctgcgtca
    13981 gtttaggtta ggctggtgtt atccctgtca aatgtaaaaa tattttttaa aataaaaaca
    14041 agctcaatca tttgtgtctt attttagaag tgcggtatta acggaaatgg aaaaagatta
    14101 tttttgcagc acaaatttga ggttctttac tgatgataag ccgtagactt ttgcgtatca
    14161 aagccatgat ggccctgtat gcgtttaaca ggagagagga tggaaatctt gaacaggctg
    14221 agaaggagct gatgttcagt atagataaaa catacgatct ctaccatctt ctgctgctgc
    14281 ttattcttga ggttgccgat gttgctgccg gcaaaatcga tcaggccctt cagaaacata
    14341 tacccactca cgaggatctg aacccgaaaa gaaagtttat tgataatctg gtcatcgccc
    14401 agttgcggaa aaacaagcca ttcaatgatt atatttcctc aaggaagctt tcctgggcaa
    14461 actatcagca tattcccagg ctgatgtacg caagtatgat agcctggaag aaatatgatt
    14521 catacatgtc ttctgaggaa tacggttatt cgggcgacag gaagttcata ataagcctgg
    14581 tgaccgaact ttttgcggcc tctgaagacc tagaggcaaa cctcgaggag cagagcatct
    14641 actggaacga tgatacagaa tatgtcgtcc tgatgatcga gaaaacactg aagaagttca
    14701 agcctgattc gggtaatgat accaaactca tgcca
//
LOCUS       DAOOYG010000064        14113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4369_length_14113_cov_7.479229, whole genome shotgun sequence.
ACCESSION   DAOOYG010000064 DAOOYG010000000
VERSION     DAOOYG010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4369_length_14113_cov_7.479229"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PK288_06925"
     CDS             <1..533
                     /locus_tag="PK288_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18692.1"
                     /translation="QAVTLSGTGGIEFGTGYTNSPHTSTASCTDCHMATPNRTSGGHS
                     FIAKNNFNGCNTTGCHSGMSASNPSFTATKAEITALIAQLADKINALGGGHDILQKDP
                     KDGSYHGYIDIYDAGANPTGYWRNPSLGSPAFPALTNAQFGAIINFQILVHDGSSGAH
                     NYSYIKKLLENTIAAI"
     gene            complement(605..1024)
                     /locus_tag="PK288_06930"
     CDS             complement(605..1024)
                     /locus_tag="PK288_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPM18693.1"
                     /translation="MEKTKKILIHSVVGIAAFTFASWGLFAQEKQAKPWEVPAEYKNM
                     ANPVPKTDESIKAGQGLYVKYCASCHGKTGLGDGVKARMLKDYAGDFSGAYYQNQTDG
                     EHFYKTKFGRNEMPKYEGKLSDENIWHMVNYMRTFKK"
     gene            complement(1240..2739)
                     /locus_tag="PK288_06935"
     CDS             complement(1240..2739)
                     /locus_tag="PK288_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HPM18694.1"
                     /translation="MKLTSLLRNWISIIGLLIASVNLILIIVLFIISAVLDKSATVLG
                     LFIYIILPGFMLFGLIMVPVGILAARKRKASASPEGIKRLPSIDLNNPVHKKAFIIFA
                     VVTVIILFLSTYGSFEAYHLTESVEFCGTLCHKVMEPEYTAYQNSPHANVACVDCHVG
                     AGASWYVKSKLSGLYQVYATITKSYQTPIETPLHDLRPARETCERCHWPQKFYSRKIF
                     TKKYFLTDSLNSEWDIMLLMKTGPEYENLGFTEGIHWHINPDVKIEYVSENDKREIIS
                     YVKYTNKETGEVTEYRIPDSEVSDSALASASPRTMDCIDCHNRPSHNYYSPPAYFDRI
                     MQAGGVSKEIPFIKKSAMEILSNTFTDRDTAMMQIRDGILNFYESGYAGYLSDNVEKI
                     ERSITSVQKAFSRNTFPAMGVNYSVYPDHAGHLESEGCFRCHNDAFESETGRRITRDC
                     NICHIIISQGTPGSMQYSAVREPLEFIHPVDIGDVWQEANCSECHKFLY"
     gene            complement(3008..3412)
                     /locus_tag="PK288_06940"
     CDS             complement(3008..3412)
                     /locus_tag="PK288_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPM18695.1"
                     /translation="MKKRLVRTVMVIAGVSFLCLYVNAQQPKPWDVPAEYKNMKNPVA
                     KGEESNKAGLATYTKFCASCHGKTGLGDGVKARMLKDFPGDFSGDYFQNQTDGEHFYK
                     TKFGRNEMPKFEGKLSDENIWNLVNFMRTFKK"
     gene            complement(3517..4143)
                     /locus_tag="PK288_06945"
     CDS             complement(3517..4143)
                     /locus_tag="PK288_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HPM18696.1"
                     /translation="MSHKMYLYPKWVRGWHVLNAVLFLVLIVTGLSMQYSGKDSIFII
                     GFANAVKWHNFAAIVLTINYIVFVTGNLTTRNGRYYRISKQNFWSDLLIQLKYYSMGM
                     FKGEKHPFPVTEQSKFNPLQKFTYVLAMYLALPLLIISGFGLLFPEITIKRFAGVSGL
                     ILTDILHITMGFLLSVFMVIHIYTCTLGTKPWSLFKGIITGYHESDEL"
     gene            complement(4133..5143)
                     /locus_tag="PK288_06950"
     CDS             complement(4133..5143)
                     /locus_tag="PK288_06950"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPM18697.1"
                     /translation="MKFWRTWMLVPFLSLLPAGSHGAWFPADAALTGGAEADTSEFIS
                     VFAEDNERCFVCHGQNRYEYENPNLGRKVRALMSKERIIDRKAFYESNHRSFSCTDCH
                     SEMYTEFPHPGELRMEQGFNCIDCHGGDENWAHFHFDEIEIEYQNSVHFKLEEEGFSC
                     WKCHGPHDYKISVRNSGNLKEAILYDNNICLDCHSNFNRFQILSDREEINILARHDWL
                     PSQATHFKSVRCIECHTQVNDTILVAHQINPKEMAVKNCTECHSRNSLLMSSLYKFQS
                     KEQRKDGFLNGVILNESYVIGANRNTFLNRISVVVFILTLGVVGVHIVFRILKNKKAD
                     ES"
     gene            complement(5161..6024)
                     /locus_tag="PK288_06955"
     CDS             complement(5161..6024)
                     /locus_tag="PK288_06955"
                     /inference="COORDINATES: protein motif:HMM:NF040196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HPM18698.1"
                     /translation="MKKTFLLLLLFAFIVSVSAQEGEKAKEKSKPVRFTFGTGLLIDN
                     QTVETNFKGSLEFQIHHRFSLIENYHNLYGIYGAANTRLGLNYGVTERLMIGVGTTKD
                     YKLQDIQWKYLILQQTEDNSMPVSMSYYGNFVLDLQKKEAFGPVERYRDIHRFSYFNQ
                     LIVARKFNDVFSFQIAPSVIYFNAVPQYTNELKYKNLNFGLSAGGKVAVKGAHSVIFE
                     YDQLLNKQQLDEQPKPNLSLGWEIGTATHNFQIFVANYSQIIYQRNLVFNTNDFRKGD
                     YLVGFNIVVRF"
     gene            complement(6054..6431)
                     /locus_tag="PK288_06960"
     CDS             complement(6054..6431)
                     /locus_tag="PK288_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18699.1"
                     /translation="MKLKALISLPSLIKVLFLSGAVFMASCEKYAYLIETVNPDEPVL
                     FQTQIQPIFTNNCITCHKGARNPDLREGNSYASLTEGGYVTQPAESSKLYKKLLEGSH
                     ASFTIDIEKQLILTWIKQGAQDN"
     gene            complement(6688..8469)
                     /locus_tag="PK288_06965"
     CDS             complement(6688..8469)
                     /locus_tag="PK288_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPM18700.1"
                     /translation="MMETRNIIKSGEFLVREVENKDIFIPEEFDEEQRMIAATCSDFL
                     ESEVFPNLQAVEKGDRELMKSILKKSGDLGMMGISIPEEYGGFGQSFVTQMLVAETTG
                     AGYSFSVAYMAHCGIGTLPIMYYGNEDQRQRYVTRLATGELIGAYCLTEPGAGSDANS
                     GKTSARLSDDGRHYIINGQKMWITNAGFADTLVVFAKIDNDRVLSAFIIDADSPGVVI
                     GPDEHKMGIKGSSTAQIYFNDVMVPVENLLGKRGEGFRIALSILHMGRIKLGANVIGA
                     AKKAITDSVKYANERKQFGVQISTFGAIKHKLAEQVIRLFASESAVYRVSSDIDILME
                     KLKSEYGDKGRASIEAISHYAVEAALLKVVGSEMLDYIADEAVQIHGGMGYSSEMDVE
                     RGYRDSRINRIFEGTNEINRLLVADTAMKRAMKGDFDLFGEAEKLFAGLESLTSEKPA
                     GEGYFAEKYRYLKNFKKIILLCMHAATKQFGKNLVTEQEVMNNITDMMMEAYLSESLA
                     LRIEKLENRRGMVTEYRDILDVNIYDTAGKIRKSAADAVYSIDLKESVPGLIKAVELL
                     SEVEGINIKNSRRRIADKLIADNMYKF"
     gene            complement(8501..10576)
                     /locus_tag="PK288_06970"
     CDS             complement(8501..10576)
                     /locus_tag="PK288_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPM18701.1"
                     /translation="MTRQIIFALTLLITLGVFYFTIVRIVRFFRFTRPAFPVRDFGKR
                     FSLMMKVAFGQSRIFRKPILGLFHALVFWGFCIILFGSIEMVIDGLSGTERALRFLGP
                     VYDFIIASGDIFAVLVAISIIVFLARRLFFRVRRFEGVEMKSKSHIDANISLTLILLL
                     MISLLTMNTGYVGYNHSEGREIYGIFPVSSILAGMMQGFSVRDFLMMHEISWWTHILL
                     IFVFANYLPYSKHFHVFMSVPNVFLSRLDPLGKLENMESITREVRLMMDPGTAYAPAA
                     DTSPGRFGVKDAEDVTWKNYFDSLSCTECGRCTEVCPANITGKKLSPRKIMMDLRARM
                     KEKGPGMVREGRGFDDGRSLLGNYVSEEELWACTTCNACARECPININHPSLIVDMRR
                     YLVLEEASAPGELKSMFSYIENNGAPWQYPADDRLNWTRDLKFRVPVLSELAGRGEKP
                     EYLFWVGCAGAFDDRYRKVVRAFAEILNHIGVSYAVLGKEETCTGDPAKRAGNELLFQ
                     MQAFQVIDLLQKYGVRKIITTCPHCFNIFKNEYPGLGGDFETISHVSFLNDRLRDGSL
                     RLSGSSLKDVRITYHDPCYLGRANEIYSEPRALLKAVTGDLAEMKSHGSFALCCGAGG
                     AQMFKEAEKGDQEIFIRRTADALETGAEIIATACPFCMTMLTDGLKYKNREEEVRNMD
                     IAELIAGSL"
     gene            complement(10839..11810)
                     /locus_tag="PK288_06975"
     CDS             complement(10839..11810)
                     /locus_tag="PK288_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPM18702.1"
                     /translation="MSVLVFTENWDGKFKRLSFELVSYASSLAGMLNTSVTAVTIGKA
                     EGDELKKLGEFGADRILNITCPQLSFLDNRAYATIISEVARKENSGVILFSNNNTGKA
                     LAPRVAVRLKAGMASGVNGLPSGVEPFVVRKRAFSGNAFAHVVIKTPYKVITLAQNSF
                     EPASRPTGAIPEEMEVSIDGSSVKASILETQKQTGRIVLTDADIVVSGGRGMRSPDNW
                     GPLVELATLLGGATACSRPVSDEGWRPHEEHTGQTGKIIAPNLYIAVGISGATQHLAG
                     VSASKYIVAINTDKDAPIFETAQYGIVGDAMKVLPKLVETVRNIRKH"
     gene            complement(11830..12579)
                     /locus_tag="PK288_06980"
     CDS             complement(11830..12579)
                     /locus_tag="PK288_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPM18703.1"
                     /translation="MKILVCISNVPDTTTKIKFTGDNKFLDTTGIQWVINPWDELSLT
                     RALELKDDPKSGVKSVTVLHVGGAGSEPTIRKALAIGADDAVRVNAEPSDAYFVAGQI
                     AEIVNREKYDIILCGIESSDYNGSEVGGMTAEFLDIPSFSSVSAIMIEGGAPVLTREI
                     DGGKEIIATECPFVAVVQKGIAKEPRIASMRGIMSARSKQIRVFEPVQVEALTEIDAF
                     EKPQPRAACKFVDPENPAELIDLLQNEAKVI"
     gene            12882..13805
                     /locus_tag="PK288_06985"
     CDS             12882..13805
                     /locus_tag="PK288_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPM18704.1"
                     /translation="MNNNLRIYGSVILSMIFWAFSFIWFKLANKTFGPITIVFFRLVI
                     SVVLLSSYLYIKKKFIRIKREDFRFFLMLAVFEPFFYFLGESFGLTYVSATVGSVLIS
                     TIPVVAAISAWIIFRERLRIINYIGIVLSFVGVLIFIINWDGTITYNAKGISLLMLAV
                     ISAAGYNLTLSRLVGSYSPVFIVNFQNCLGSVLFLPLFMGFELKHLTAASFSVSSLMP
                     VIALAIFASCFAFILFAYSVRHMGVTRANVFTNFIPVFTAVFSFIILGDKLTFQNIAG
                     MVIVISGLLMSQINGRKKKYDEALVLTGKTA"
BASE COUNT         3652 a         3493 c         2924 g         4044 t
ORIGIN      
        1 cacaggccgt tactctttca ggtaccggag ggattgagtt cggaacggga tacacaaact
       61 ctccacatac ttcaacagca tcctgtaccg attgccatat ggccactccc aacaggacat
      121 ccggcggcca ttcattcatc gcaaagaata atttcaacgg ctgcaatacc acaggatgtc
      181 acagcggcat gagtgcctcc aatccatcct tcaccgcaac aaaagccgaa atcactgccc
      241 ttattgcaca gcttgccgac aagatcaatg cactgggcgg cggtcatgac atactccaga
      301 aagatcctaa agacggaagt taccatggtt acatcgatat ctacgatgcc ggtgccaatc
      361 cgaccggcta ctggagaaat ccgtcacttg gaagtcccgc attccctgcc ctcaccaacg
      421 cacagttcgg agccattatc aatttccaga tcctggtcca cgacggcagt tccggtgcgc
      481 ataactattc ttatatcaag aagcttcttg agaacacaat tgccgcaata tagaaaggca
      541 taaatgcata aagaaaaaga gagccgcccc gattggggcg gctctctttt tctcggctca
      601 ttgtttattt cttgaatgtg cgcatgtaat tcaccatgtg ccagatattc tcatcactta
      661 atttaccctc atatttcggc atttcgttcc tgccaaactt tgtcttgtag aaatgctctc
      721 catcagtctg gttctgataa taggcaccag agaaatcacc ggcataatcc ttgagcattc
      781 ttgcttttac cccgtcaccc agaccggttt ttccatggca tgaagcgcag tatttaacat
      841 aaaggccctg gcctgcttta atgctttcat ctgtcttggg caccggatta gccatgttct
      901 tatattcggc aggaacctcc cagggttttg cctgtttttc ctgggcaaaa aggccccatg
      961 aagcaaatgt aaatgcagca atgcctacaa ctgagtgaat caaaatcttt tttgtttttt
     1021 ccataataga tttatttaag tatttctgca atgtttacaa taaccgtgca aatttatcaa
     1081 aagttactta aaaaccatca gatggtgcct tttatgatgt tttgtgaaaa aaataacagc
     1141 tgatccttcc ggcaggaaac cgtataaagc cgttaccgac tggtattttc acggtatgga
     1201 tgccgcctgc aggcttaaca agaatgctgc tcctgtgttc taatacagga atttatggca
     1261 ttcggaacag tttgcttcct gccatacgtc tccgatatca acagggtgaa taaattcaag
     1321 cggctccctg actgccgaat attgcatcga tccgggtgta ccctggctga taataatgtg
     1381 acatatattg caatcgcggg taatccttct gccggtctca gattcgaagg catcattatg
     1441 acacctgaag cagccttcag attcaagatg gccggcatga tcaggataaa cactataatt
     1501 cactcccatt gccgggaaag tgtttcttga aaaggctttc tggactgaag tgattgaccg
     1561 ttcaatcttt tcaacgttat ctgacaaata acccgcataa cctgattcgt agaaattcag
     1621 tattccatcc cttatctgca tcattgctgt atccctgtcg gtgaaggtat ttgaaagtat
     1681 ctccattgcc gactttttta tgaaggggat ctccttcgat acgccgcctg cctgcattat
     1741 cctgtcaaaa tatgccggag gcgaataata attatgggat ggcctgttat gacagtcaat
     1801 gcaatccatt gtacggggag aggctgatgc caatgcgctg tcagacactt cagaatccgg
     1861 gatacggtat tctgtcactt ctcctgtttc cttattggta tatttcacat aactgattat
     1921 ctccctttta tcattctcgg atacatattc aatcttaaca tcgggattta tgtgccagtg
     1981 aattccttcg gtaaacccaa gattctcata ttcagggccc gttttcatca gcagcatgat
     2041 atcccattcg gaattcagag aatcggtcag aaaatatttt tttgtaaaaa tctttcgcga
     2101 atagaatttc tgcggccagt gacagcgttc acacgtctcc cttgcaggcc gcagatcatg
     2161 cagcggagtt tcaatggggg tctgatagct tttggtaatg gttgcatata cctggtacaa
     2221 ccctgacaat ttcgatttga cataccacga tgcgccggct ccaacatggc agtcgacgca
     2281 agccacgttg gcatgtggtg aattctggta tgcagtatat tccggttcca taaccttgtg
     2341 gcacagggta ccgcaaaact ctaccgattc tgtcaggtga tatgcctcaa aactgccata
     2401 cgtcgacagg aaaagaataa ttacggttac aacggcaaaa atgatgaagg ctttcttatg
     2461 tacagggttg ttaagatcaa tgctgggaag cctctttatc ccctcgggag aagcagatgc
     2521 ttttcttttc ctggcagcca gtataccgac aggcaccata atcagaccaa acagcatgaa
     2581 gcccggaagg attatataaa tgaacaaacc aaggacggtt gcacttttat cgagcaccgc
     2641 tgatataatg aacaaaacaa ttatcaggat cagattgact gatgcaatca gcaacccgat
     2701 aatggatatc caattcctga gtaaagacgt cagtttcata gtgatctgtt atttcaggtt
     2761 aatcagttac agcaatgaca gtgaattaag tttctgattc caataacccg ggattcagat
     2821 aagtgatgat ctgtaaggta cattgcaact ccgatcaaat aactcgggtc atccttcctg
     2881 agtgagccgg caacagaaca aaaacgggaa tgaattcagg tgcaattcat ccccgtgagc
     2941 cgaaaacaaa agctcatcaa tattattctt ccttcaaaaa aaccagatcc gggtattgcc
     3001 cccaattcta ttttttgaat gttctcataa agttaaccag attccagata ttttcgtcgc
     3061 ttaatttgcc ttcaaatttg ggcatttcat tccggccgaa tttagtttta tagaaatgct
     3121 ccccgtcggt ctggttctgg aaataatcac ctgaaaaatc gcccggaaaa tccttaagca
     3181 ttctggcctt gacgccatcg cccaaaccgg tttttccatg gcatgatgca caaaatttcg
     3241 tataggttgc caaacctgcc ttgttgcttt cttctccttt ggcaacagga tttttcatgt
     3301 tcttatattc ggcaggaaca tcccagggtt tgggttgctg cgcatttaca taaaggcaaa
     3361 ggaatgacac tccggcaatt accattactg ttctcacaag tcgcttcttc atagtaatca
     3421 tagtcgtgtt ttttaagtta gcaattcagt acaaatatcg acaaatggct caatcaacat
     3481 tcaatatatt gaaatatatt gacagagata cagatattaa agttcgtccg attcatggta
     3541 gccggtgatg atgcccttga agagcgacca gggctttgta cccagggtac aggtatagat
     3601 atggatcacc atgaataccg acagcaggaa acccattgta atgtgaagga tatccgtaag
     3661 gataaggccg ctgacccctg caaatctctt tatggttatt tcaggaaata acaggccaaa
     3721 tcctgaaatg atcagcagcg gcaatgccag atacattgcc aggacatagg taaacttctg
     3781 cagaggattg aatttactct gctcagtcac gggaaacggg tgcttttctc ccttaaacat
     3841 tcccatggaa taatatttaa gctgaatcag caggtcggac cagaaattct gttttgagat
     3901 cctgtagtac ctgccattcc ttgttgtaag attcccggtc acaaagacaa tatagttgat
     3961 agtcagtaca atcgcggcga agttatgcca ctttacggca tttgcaaatc cgatgataaa
     4021 gatgctgtcc ttaccggaat actgcatgct gagcccggtc acaataagga ccaggaacaa
     4081 aacggcgttg agaacatgcc agccccttac ccacttggga tacaggtaca ttttatgact
     4141 catcggcttt tttgttttta aggattctga aaacaatatg aactccaaca acacccagtg
     4201 tcagtataaa aaccaccacg cttatgcggt taagaaatgt gttcctgttt gcgccgatca
     4261 catacgactc attgagaatt acgccgttca ggaatccgtc cttccgctgc tccttcgact
     4321 gaaatttata aagcgacgac ataagcagtg agttacgcga atgacattca gtgcagtttt
     4381 ttacagccat ctccttcggg ttgatctggt gtgcaacaag gatggtgtca ttgacctggg
     4441 tatggcattc aatgcatctt acgcttttaa agtgcgtagc ctggctcggc agccagtcgt
     4501 gcctggcaag aatgttaatt tcctccctgt cagacagtat ctggaaccgg ttaaaattcg
     4561 agtggcagtc aaggcagata ttgttatcgt aaagtatcgc ctctttcagg ttaccgctgt
     4621 tcctgaccga gattttataa tcatgcggcc cgtggcactt ccagcatgaa aacccctctt
     4681 cctcaagctt gaagtgtacg ctgttctgat attcaatctc aatttcatca aaatggaaat
     4741 gtgcccagtt ttcatcgccc ccgtggcagt caatacagtt gaaaccctgt tccatcctta
     4801 attccccggg atgcggaaac tcggtataca tttccgaatg gcagtcggtg cagctgaagc
     4861 ttcggtggtt cgactcataa aatgctttcc tgtcaattat cctctccttc gacatcaatg
     4921 cccttacttt cctgccgaga ttgggatttt catattcata acggttctgg ccatgacaga
     4981 caaaacaccg ttcattatcc tctgcaaata cggaaatgaa ttcagatgta tcagcttccg
     5041 ctccgcctgt taatgctgca tcagctggga accaggcacc atggcttccg gccggcagca
     5101 aggacaggaa gggcacaagc atccaggtcc tccagaattt cattattttc ctgaaattat
     5161 tcagaatctt acaactatat tgaatcctac caggtaatca ccttttcgga aatcgttcgt
     5221 attgaatacc aggttccgct gataaatgat ctgtgagtaa ttggcaacga atatctggaa
     5281 attatgggtg gctgttccaa tctcccatcc cagcgaaaga tttggtttcg gctgctcatc
     5341 aagctgctgc ttgttcagaa gctgatcata ttcaaaaata accgagtgtg ctcccttaac
     5401 ggcaactttt cctccggccg acaagccaaa gttcaggttt ttgtatttta actcgttggt
     5461 atactgggga acggcattga aataaatcac tgaaggggca atctgaaacg aaaaaacgtc
     5521 attgaatttc cttgccacaa tgagctggtt aaaataggag aacctgtgaa tgtccctgta
     5581 acgctcaacc ggtccgaaag cctccttttt ctgaagatcg agcacaaaat tcccgtaata
     5641 cgacattgaa acaggcatgc tgttgtcctc ggtctgctga agtataagat atttccactg
     5701 aatatcctgc aatttataat ccttggttgt acccacacct atcatcagac gctccgtcac
     5761 accgtagtta agacccagcc ttgtgttggc cgcaccgtat atcccataca gattatggta
     5821 gttttcaatc agactgaagc ggtgatgtat ctgaaactca agacttcctt tgaaattcgt
     5881 ttcaacagtc tgattatcta tcagcagccc tgttccaaag gtaaatctga ccggttttga
     5941 tttttctttt gctttttctc cttcctgggc cgaaacggaa actatgaagg caaataacag
     6001 cagcaatagg aaagtctttt tcatcttatc gggtttttat ggttttctgg cttttaatta
     6061 tcctgtgcgc cctgttttat ccaggtgagt atcaattgct tctcgatatc aattgtaaac
     6121 gaggcatggc ttccttcaag cagtttctta tatagtttgc tcgattccgc cggctgggtc
     6181 acatatccgc cttcagtaag agatgcatag gagtttcctt ccctgaggtc gggattccgg
     6241 gctcccttgt gacaggtaat acaattgttg gtaaaaattg gttgtatctg ggtctggaaa
     6301 agaacaggct catccggatt taccgtttca atcaggtagg catacttctc gcatgatgcc
     6361 atgaatacag ctcctgaaag aaataatacc tttatcaggc tgggtaatga tattagagct
     6421 tttaatttca tctctcggct tttgttaatt atttcacaaa tatatcccag attacatacc
     6481 aatcgcaaca tggatactgc atttttaact ttaattaaat gttctttatc acatgttgtt
     6541 ttgatctaag tcatcagtaa atatgataaa tatcaactac attaccgttc attaacatat
     6601 atcccggtaa aaaccaacct gagggaacaa tccgtttccg gggatttgtt taacaacacc
     6661 cggcctgcaa attccggatc gcaaggttta aaacttgtac atattatcgg caataagctt
     6721 gtcggcgatt cttcttctgg agttctttat gttgatgcct tcaacttcag aaagcagctc
     6781 cactgccttt atcaatccgg gaaccgattc cttcaaatca attgaataaa cggcatcggc
     6841 tgccgatttc ctgattttcc cggcagtgtc gtatatattc acatcaagaa tatcccggta
     6901 ctcagttacc attcccctgc gattttccag cttttcgatc ctcaatgcaa gcgattcgga
     6961 aagatacgct tccatcatca tgtcggtgat attgttcatg acctcctgct cagtcacgag
     7021 gtttttcccg aattgtttcg ttgcagcatg catacagagc aggatgattt tcttgaaatt
     7081 tttcagatat ctgtattttt cagcgaaata gccttcacct gcaggctttt cactggtcag
     7141 gctttcaagt ccggcaaaca gtttttctgc ttcgccgaaa agatcaaaat cgcctttcat
     7201 ggctcttttc attgcagtat cggctaccag cagcctgttt atttcatttg tcccttcaaa
     7261 gatcctgttt atccttgaat cgcggtaacc cctttccaca tccatttcgg aagaataccc
     7321 cattccgcca tggatctgga cagcctcatc ggcaatatag tcgagcattt ccgatcccac
     7381 caccttcagc agtgcagctt caacagcata atggctgatg gcttctatgg atgcccttcc
     7441 cttgtcgccg tattctgatt taagcttttc catcagaatg tcgatatcac tgctgaccct
     7501 gtatactgcc gattctgaag cgaaaagtcg tataacctgt tctgcaagct tatgctttat
     7561 tgctccgaat gttgagatct gaaccccgaa ctgctttcgc tcgttggcat atttcacgga
     7621 atcggttata gctttttttg cagccccgat aacgttggct ccaagcttga tcctgcccat
     7681 gtgaagaatg ctgagcgcga tcctgaagcc ttctcccctt tttcccagga ggttctcaac
     7741 cggaaccatg acatcattga aatatatctg tgcggtcgac gatcccttta ttcccatttt
     7801 atgttcatcc ggtccgatta ccactccggg tgagtcagca tcaatgatga aagcacttaa
     7861 aacacggtcg ttgtcgatct tggcgaaaac aacaagggtg tctgcgaaac cggcatttgt
     7921 gatccacatt ttctgaccgt tgataatata atgcctgcca tcatcggaaa gccttgcact
     7981 ggtctttccg gaatttgcat cgctcccggc cccgggttcg gtcaggcagt aagcgcctat
     8041 aagttcgccg gttgcaagcc tggtgacata acgctgccgc tggtcctcat ttccgtaata
     8101 cattatgggc agggttccga tgccgcagtg agccatatat gcaaccgaga atgaataacc
     8161 tgcgcctgtg gtctcagcca ccagcatctg ggtaacaaac gactgaccaa accctccgta
     8221 ttcttccgga atggaaattc ccatcatccc gaggtctcca gattttttca gaattgactt
     8281 catcagttca cggtcgccct tttcaacagc ctgaagattc ggaaatacct cagattcgag
     8341 aaaatcactg caggtcgcgg caatcattct ctgttcctcg tcaaactcct cgggtatgaa
     8401 aatatccttg ttttcaactt cccttacaag gaattctccg cttttaataa tattcctggt
     8461 ttccatcatt gtatctttgg ttttttattt aattccgtca tcaaagcgat ccggcaatca
     8521 gttcagcaat atccatattc ctgacctctt cctcccggtt tttgtatttc agaccgtcgg
     8581 tcagcattgt catgcagaaa gggcaggccg tggcaatgat ctctgcgccg gtttcaagtg
     8641 catctgcagt gcgccttata aaaatttcct gatccccttt ctccgcctcc ttgaacattt
     8701 gtgcacctcc cgcaccgcag cataaggcaa aactcccgtg actcttcatc tctgccagat
     8761 cacccgtaac ggctttcagc agggcacgcg gctctgaata aatttcattt gccctgccaa
     8821 ggtaacaggg atcgtgatag gtgatcctga catccttcaa cgagcttccc gataatcgga
     8881 gcgacccgtc cctcagcctg tcattcagaa agctgacatg gcttatcgtt tcaaaatccc
     8941 ctcccaggcc gggatattca ttcttaaata tattgaaaca gtgcgggcag gttgttatga
     9001 tcttcctgac cccgtatttc tgcaggagat caataacctg gaatgcctgc atctggaaca
     9061 gcaactcatt tccagcccgt ttggccggat cgcctgtgca ggtctcctcc ttgcccagca
     9121 cggcatagct cactccgata tggttcagta tttcggcaaa agctcttaca accttcctgt
     9181 acctgtcatc gaatgcgccc gcacagccga cccagaataa atattcgggc ttttctcccc
     9241 ggcctgccag ctcagacaga accgggaccc ggaatttcag atccctggtc cagttcagcc
     9301 ggtcatctgc aggatactgc cacggagctc cgttattttc aatatagctg aacatgctct
     9361 taagctcccc tggagcagat gcctcttcaa gtaccaggta ccgcctcatg tcaactatca
     9421 gggaaggatg attgatgttg atggggcact ccctggcaca ggcattgcat gtggtgcatg
     9481 cccataattc ttcttcggaa acatagtttc ccagaagcga cctgccgtca tcgaatcccc
     9541 tgccctccct caccattcct ggaccttttt ccttcatcct tgcacgcagg tccatcatta
     9601 tcttgcgggg tgaaagcttt ttacccgtaa tattcgcagg gcaaacctca gtacatctcc
     9661 cgcactcggt acatgacagc gaatcaaagt aattcttcca tgtaacatct tctgcatcct
     9721 tcaccccaaa tctcccgggc gaggtgtctg ccgccggagc ataggccgtc cccggatcca
     9781 tcataagcct gacctccctg gtaatgcttt ccatgttctc cagcttcccg agcgggtcga
     9841 gtcttgaaag aaaaacattc ggtaccgaca tgaacacatg aaagtgtttt gagtatggca
     9901 ggtagttggc aaacacgaaa atgaggagaa tgtgagtcca ccacgatatc tcgtgcatca
     9961 tcaaaaaatc cctgacggaa aatccctgca tcattcctgc caggatgctg ctgaccggaa
    10021 aaataccgta aatctccctt ccttctgaat gattgtaccc gacataccct gtattcatcg
    10081 taagcagcga gatcataagc agcagaatca gcgtaagaga gatattggca tcaatatgag
    10141 atttactctt catctccacc ccttcgaatc tcctgaccct gaagaacagg cgcctggcaa
    10201 ggaagacaat tattgagatt gccaccagga cggcaaaaat atcacccgaa gcaataatga
    10261 aatcataaac agggcccagg aatctgaggg cacgttctgt ccccgaaaga ccatcgatca
    10321 ccatttcaat gcttccaaag agaattatgc agaagcccca gaaaacaaga gcatgaaata
    10381 atcccaggat cggttttctg aaaatccttg actggccgaa tgccactttc atcatgaggc
    10441 tgaatcgctt cccgaaatca cgcacgggga atgccggacg cgtaaaacgg aagaagcgta
    10501 caattcttac tatcgtgaaa taaaacactc ccagtgtgat caggagtgtc agggcaaaaa
    10561 taatctgtcg ggtcattgat caggtatttt gtattttcag gttattgtca gtattccctt
    10621 gaattcaatt aggcggacct ggattgaaag ttacggagag gacacaactg aaatgactgt
    10681 catcggataa ttgcttcagg aacttaaatt cagatagttt tttcctgtac cggattcccc
    10741 ggcaggactc cctgcttaac aggacccgtt tcagcaactc cgcggttttc ggcagattcc
    10801 ttgtgcattt cagtggcaaa tcctcaggaa gctcagaatc agtgtttacg gatattcctg
    10861 acagtttcaa ccagtttcgg cagcactttc atggcatccc cgactattcc atattgtgca
    10921 gtttcaaaaa tcggggcatc cttgtcggta ttaatggcaa cgatgtactt tgaagcgctc
    10981 actcctgcca gatgctgggt ggcacccgaa atgccaaccg caatatacag gtttggtgca
    11041 atgatcttcc cggtttgccc ggtatgttct tcgtggggtc tccatccttc atctgagaca
    11101 ggccgcgagc aggctgttgc gcctcccagg agggtggcaa gttcaacaag cggaccccag
    11161 ttatccggcg acctcatgcc cctgcccccg gaaaccacga tatcagcatc ggtaagcact
    11221 atcctgcctg tttgtttttg ggtttccagt atacttgcct taacggacga tccgtcaatg
    11281 gagacctcca tttcctccgg tatggctcct gtaggtctgc tggccggttc aaacgaattc
    11341 tgcgccagtg taatgacttt gtacggggtc ttaattacaa catgtgcaaa agcatttccc
    11401 gagaaagcac gcttacgaac cacaaacggc tccacacctg aaggaagacc gttaactccc
    11461 gaagccatcc cggcctttag cctgactgca accctggggg ccagggcttt cccggtatta
    11521 ttgttggaaa acaggatgac ccctgagttt tcttttcgtg caacttcaga aattatcgtt
    11581 gcatatgccc ggttatcaag gaaagacaat tgcgggcatg tgatattcaa tatcctgtct
    11641 gcaccaaact cacccagttt tttcagctca tccccttccg ctttaccgat tgtgactgca
    11701 gtcacactgg tatttaacat accggccagg gaggaggcat atgataccaa ttcaaatgac
    11761 agtctcttga atttaccgtc ccagttttcc gtaaaaacta atactgacat gtttttctgg
    11821 ttttattttt cagatcactt tggcttcatt ctgaagaaga tcaataagct cagccggatt
    11881 ctccggatca acaaatttgc aggcggcacg cggctgcggc ttttcaaaag catcaatctc
    11941 agtcaatgcc tcaacctgaa caggttcaaa tacccttatt tgttttgaac gggccgacat
    12001 tataccccgc atcgaggcaa tccggggttc ttttgcaatt cccttctgta ccactgcaac
    12061 aaacgggcac tctgtagcaa tgatctcctt tccgccgtca atttcccttg ttagtacggg
    12121 agctccgccc tcaatcataa ttgccgagac cgatgagaat gaagggatat ccagaaattc
    12181 ggctgtcatt cctcctactt ccgaaccatt gtaatcgctt gactcaatgc cgcacaaaat
    12241 tatgtcatat ttttccctgt tcactatttc agcaatctgc cctgcaacga aatatgcgtc
    12301 ggatggttct gcattcactc ttactgcgtc atcagcacct attgcaaggg ctttcctgat
    12361 ggtgggctcg gatccggctc ctccgacgtg taatacggtt accgatttca ctccgctttt
    12421 cggatcatct ttaagctcaa gtgcgcgggt aagcgacagt tcatcccatg gatttatcac
    12481 ccattgaata ccagtggtat ccaggaattt gttgtctccg gtaaacttta tttttgtggt
    12541 ggtgtccggc acattgctta tacaaaccag gattttcatt gctaacaggt tttagatttt
    12601 attgatacac ttaggtttgt caaagtttag cgttcgaggc ggcaaatata ataatttaac
    12661 cttcataatc aaatcgcgga acggactatt attcgcatgg tcgaaataca gctgtatgtt
    12721 aattaattaa tgatctgtca attaagtcac tgaaaatctg tcttccgaac tgccgttcga
    12781 aacaataaca aattagcatt aagaataatt acggaatggc tcgatcaaat acaattttta
    12841 agattaactt cgcacgccgg taatttctct gtttaacccc tatgaacaac aacctcagga
    12901 tatatggttc tgtaatcctt tcgatgatat tctgggcatt ctcatttatc tggttcaaac
    12961 tggcaaataa aacattcggg ccaattacca ttgtgttttt caggctggtc atttcagtcg
    13021 tccttctttc gtcatacctg tacattaaga agaaattcat caggataaaa agggaggatt
    13081 tcagattctt cctgatgctg gctgtttttg agccattctt ttatttcctg ggagagagtt
    13141 tcggcctgac ctatgtttcc gcaacggttg ggtcggtact tatttctaca atcccggtgg
    13201 ttgctgccat aagcgcatgg attattttca gggaaagact caggattatc aattatatcg
    13261 ggatagtcct gtcgttcgtt ggtgttttga tattcatcat caactgggac ggcaccataa
    13321 catacaatgc aaaggggatc agcctgctga tgctggcagt catttccgcc gccggctaca
    13381 atctgaccct gagcaggctc gtaggaagtt acagtccggt attcattgtg aatttccaga
    13441 actgcttggg ttccgtcctc tttctgcccc tgttcatggg atttgaactg aagcacctta
    13501 cagccgcttc cttttcagtc agttcattaa tgcctgtcat tgcgctcgca atttttgcat
    13561 catgcttcgc attcatcctg ttcgcctatt cggtacggca catgggtgtt acaagggcaa
    13621 atgtattcac aaacttcatt ccggtattta ctgctgtctt ctcgttcatt attcttggtg
    13681 acaagctgac cttccagaat attgcaggta tggtcatagt catttccggg ctccttatgt
    13741 cgcagataaa cggccgtaag aagaagtatg acgaagcatt ggtgcttacc ggcaaaactg
    13801 cctgaccggc aaggacgggt atgaactctg catatctgtt ttaaattctg aactgataag
    13861 gggccgatac actgcttaac ttccatattt gcacgctcat ttaatttttt aaagcctggg
    13921 agcctgcccc gctccagcgg ggcttttaag tccaatgtcc ccggtaacat ccggggtagt
    13981 tctccgaccc tgctgaccaa ccccggagag ggttgaagct gctgttcttc aacacctccg
    14041 gcgttgaaag ccagggcaga catttaccat ggatgtaacc atggatattg gacttgcagc
    14101 cctccgggct ggc
//
LOCUS       DAOOYG010000065        14080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4381_length_14080_cov_6.793440, whole genome shotgun sequence.
ACCESSION   DAOOYG010000065 DAOOYG010000000
VERSION     DAOOYG010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4381_length_14080_cov_6.793440"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="PK288_06990"
     CDS             <1..1182
                     /locus_tag="PK288_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPM18705.1"
                     /translation="KTVVLLINDPGYVTSDSSLFNGRAMTYYGRWTYKYEEAARQGAE
                     AVIIIHETGAAAYPWGVVQNSWSGPEFCLEDDELASSGLQFKGWITNESARKIFGSAG
                     LDFDRLTKSASTRGFKPVDMKLKLSLELRNRTEYTRSNNVAAVWPGSRRPDEYIIYTA
                     HWDHFGVNNAFKGDSILNGAVDNATGTASLLVMAKAFTLLHPVQDRSVLFLSVTCEEQ
                     GLLGSDYYARNPLFPPGKTIGVINIDGLNIFGRTKDMNITGYGFSGLDDYAIAVLGKY
                     GRYATPDPTPEKGSYFRSDHFSFAKVGIPSLNLSSGVDNVEHGREWGLAQNEKWTMDN
                     YHKPSDNYEPDKWDFSGMIEDTRVYFEIGYDLSNTDDFPEWKPGIPYKSLRDMMMQKQ
                     N"
     gene            1287..2744
                     /locus_tag="PK288_06995"
     CDS             1287..2744
                     /locus_tag="PK288_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPM18706.1"
                     /translation="MKSADLVFSFQRAVIMCNGKLLAGDLSFDVHRGESWWVCGANGT
                     GKTTLLEIIAGKQRLAGGEMILPENVTVSEFYSSVAIVRRDFSLYQWFTGSASFYQQR
                     YFSPGVEETPGVLDLIHEKTGFSKEDICSAAHEFGFGSLLDRRIVSLSTGEGRRILLL
                     MLWLSGKKVICIDDPYEGLDPEGKQLVTDTLHSLVKKKAAVLVTGTDTSPPDFVSHVL
                     YLRNQRVGYAGEAELFNLRNADSPQPYKPPGLKRYVPDNFNRAFMTMAEMKNITIRYG
                     SNIVQENFSWRIRRGEKWLLSGPNGSGKTTLMSLIYADNPMAYAYDLVLFDRKRGTGE
                     TIWEIKEPIGYFSSELQNFFPRHLTLHESVLTGYSNHLLVRNDLTPEHYRQADGLIEA
                     AGLSALRMVPMYRLSFSQCRLALVCRALVKLPPVVILDEPCQGLDRTAAQTVNCLVDN
                     VCSGELNTLIYVTHRIEFVPGIINKHLRLVKYSQE"
     gene            complement(2863..3429)
                     /locus_tag="PK288_07000"
     CDS             complement(2863..3429)
                     /locus_tag="PK288_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HPM18707.1"
                     /translation="MELKEAILNRRTVRRFNSKAISDETLLDILEAGTWAPSHGNNQP
                     WEFILAGPETRGKLAVAYRKAMESGPLLNPDLPEERKQVIRNFSMNFGDAPVILAVTC
                     APALTDLDRYDFPMSAAAAIQNILLAAWEKQIAGVWLSFGSTPQARSLFGIPEEGKIA
                     GILALGYPEVIPPAQPRISAINKLSKLP"
     gene            complement(3516..3896)
                     /locus_tag="PK288_07005"
     CDS             complement(3516..3896)
                     /locus_tag="PK288_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPM18708.1"
                     /translation="MKTLIPEKLLEVLKHEGVVAIAMLDDNGIHMVNTWNSYLKVTTG
                     GKIIGPAGGMRTTEAIVRKNNKIVMTLGSREVQGFHSKGTGFLIHGTLAFISEGDEYN
                     ELKQRFPWARAMFEIQPDTITQTL"
     gene            4100..6466
                     /locus_tag="PK288_07010"
     CDS             4100..6466
                     /locus_tag="PK288_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPM18709.1"
                     /translation="MKKPFQKHATSLFMALILMLFGFPAVSQLNEASPGAAPYKNPSV
                     PVMLRVEDLLSRMTLAEKIGQLNMPCVYESALGNDIESRTVNCKTLAEGTFQDYLGPI
                     GGFFTLANTILHEGTLQQANFFNELQKIAVEKTRLGIPLLQTEEGTHGLMCSGGTIFP
                     EGLAIGSTWNMDLVSRMYTVAACEARSVGIHQLFTLVVEPNRDPRLGRNQEGFSEDPF
                     FCSRMAETIVNAVQGTDVSANDKVVAGLCHYPGQSQPNSGMERGAMEISERQLREVFL
                     PPWIAGVKKAGALGVMATYPAVDGIPTHASEFLLTKILRNELGFKGLVLSEGGGLNTL
                     LYTGVAENDQQAGELALRAGLDVGISFEDGYLKPLIDAVKEGRVSMELIDRSVRRVLE
                     LKFRLGLFDKPYVDPQRALDVTHTKESRELALDAAREGIVLLKNEKNLLPVRKNIRKV
                     AVIGPNADNERNQLGDYAAHVILQEITTVLDGIRSMVSPGTVVEYVKGCDVTGNKVNE
                     LARARRAASGADLAVVVLGENEWQAPDKTGTSGEGYDVASLDLTGLQEDLLRAVHSSG
                     TPVVLVLINGRPLSVRWAAENIPAIIEAWIPGEAGGTAVAEIIFGDCNPSGKLAVTIP
                     RHVGQIPFYYNYMPEKLYWMEKGWGKAYADMPATPLWEFGFGLSYTTFMYSNLKINPE
                     VTGIFGEVKVSLDVENTGKRAGRETVQLYVRDRHASVTVPVKELKGFEKISLAPGEKK
                     TVEFRLTHDDLALYNRNMEFVTEPGIFEVMAGSSSADIRLKGKFELKN"
     gene            complement(6498..8246)
                     /locus_tag="PK288_07015"
     CDS             complement(6498..8246)
                     /locus_tag="PK288_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18710.1"
                     /translation="MVPDNNPSVTEIKKLEKYSSAFTLSDMEIFIFPELFFPLVIANI
                     MSPVIWKWRDDPWFSNIESKSFNYKVNRIKQFIIDNYVFNLDLSTWGLTTKTREMSRF
                     SDFFDLEIMKQSNALFGYEGDKYYFDIDIRKHFGLDKYDSEVIPYWKTETVEAMTAFR
                     YKDGFTTGAGECVSLSSLYAAALFIVGRIPLEKIFLIATPLHSQNFVTEKEGLITNNR
                     RIVTKNMWFNGTSLSQKARRALENEKITMVTHITGFIHIIYNEATISRKDYETFSTKL
                     KSFLRTELTDTIFINFLRFKPRYKSLFQYYCDCSGKHLYISLEKMFEYEHTSKHNLTK
                     ETREQLVNEIEGDEFHLSPINGKILLNNVEKIFQENKSISIDEFIKEFEKLAGSVPSS
                     LLDEMFSDLRVFIVTDPRLPDPAKSFRKSLEPEIGIDDTRESILKKINDMAGISETAL
                     LSLYVYRQMDMIDWVPFIKAAIERNPVCFKDLEEKDLNEVYGILMDLENESIYDEKRL
                     ALPDEVWNFQRGDGIEKALLLADLIVHRDGSNQITLEINGSDVTLEHMGTNYSFISGK
                     ALVKSVKIQGNSYSAE"
     gene            complement(8356..8778)
                     /gene="ndk"
                     /locus_tag="PK288_07020"
     CDS             complement(8356..8778)
                     /gene="ndk"
                     /locus_tag="PK288_07020"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPM18711.1"
                     /translation="MNGEITFSIIKPDAFGSGNTGPILAMIHEAGFEIVALKLLRMTR
                     EQAKSFYAIHKDRPFYNDLVEFMISGPVIVMILRQENAVDKFRKLIGATDPAKAEPGT
                     VRKRFASSVQMNAVHGSDSDENALRESNFFFSEAERCL"
     gene            8852..9889
                     /locus_tag="PK288_07025"
     CDS             8852..9889
                     /locus_tag="PK288_07025"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPM18712.1"
                     /translation="MEKVIDPAKYTKELSKLIASSDNILIICHINPDGDAIGSQLALF
                     HYLTSKGRHVDMISPNNLQEFLKWMPGADQIRIFIRQRAECIALIKQADLIIMTDFSH
                     PGRLGEAEKYVISSGAKKVIIDHHLDPADFTDLTISDPSKCATTELVYELITLMDGVP
                     YTDNKFSECIYVGIISDTGNFDHGALTGRTFRIVADLLDNGINREKMINVVFNNYSAD
                     RMRLMGLALNKRMVIIPELKTAYIYLTRRDLTDFNHVKGDTEGFVNLPLSIRDIVFSV
                     LFIEKENHVKISFRSKGNFPVNKFASEYFSGGGHMNASGGEYYENLDNTIAYFLRILN
                     DNAALIKNATV"
     gene            9876..10409
                     /locus_tag="PK288_07030"
     CDS             9876..10409
                     /locus_tag="PK288_07030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPM18713.1"
                     /translation="MQPFKYYRICIFTFFLSVLLISCRGRNEAERVVSGPGKNDMTEL
                     NRYFVQKDREIIENYIERKGLGMQESPTGLWYFLRKEGRGNLISDNKKVRIAYECSLL
                     DGTKCYSSDDSGPKEIVLGKTGIEQGLYEGLKMLRPGAEAIFIIPPFLGHGLIGDGRK
                     IPPRAVIVYEVKVLSVE"
     gene            complement(10532..11212)
                     /locus_tag="PK288_07035"
     CDS             complement(10532..11212)
                     /locus_tag="PK288_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPM18714.1"
                     /translation="MIPVTLGSDDYRLLIPDKVIGITRSLRVFIVENVRTARRFLRLI
                     DTEFPIGDTLFVELNEHTPEPDIIRCLDPVIDGRDAGLMSEAGMPGIADPGARVVSLA
                     HQKKIRVIPLSGPSSITMALMASGLNGQNFAFHGYLPVRDKERIAKIRELETKSRSGQ
                     AQIFMETPYRNQKILESLLDVCSSDRKLCIASDISLHSESIKTKTIGEWRKNIPDIKN
                     HPSIFILQ"
     gene            complement(11290..11535)
                     /locus_tag="PK288_07040"
     CDS             complement(11290..11535)
                     /locus_tag="PK288_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18715.1"
                     /translation="MECSINIGRGSHIGKLFISRISFQSLVLSIGVLYEYFRKITEYQ
                     IIKVVSGKHHVGKTDPSGYNTTEWKVLPEMAQQTKPE"
     gene            11721..12488
                     /locus_tag="PK288_07045"
     CDS             11721..12488
                     /locus_tag="PK288_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM18716.1"
                     /translation="MKITFLGTGTSQGVPVIACECDTCTSDDIHDKRLRTSILVEKDE
                     TCIVIDAGPDFRQQMLRENVKKLDAILLTHEHKDHIAGMDDVRAYNYRSRDAIDIYAE
                     ERVQKAVRKEYSYVFAEYQYPGIPKMRLNLIPEYGFEVGSIGVLPLRVYHYRLPVYGF
                     RIGNFAYITDANYVPEETKEKIFGVKYLVINALRKEKHISHFSLREAIDFIRQISPRK
                     AFITHIGHQMGRHDEVSAGLPAGIMLAYDGLRLVCDE"
     gene            complement(12502..12639)
                     /locus_tag="PK288_07050"
     CDS             complement(12502..12639)
                     /locus_tag="PK288_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18717.1"
                     /translation="MPARRAPAHSKKMTTQEASTTPMGRNRYYYSPSYQKKLNKNFRR
                     K"
     gene            13128..>14080
                     /locus_tag="PK288_07055"
     CDS             13128..>14080
                     /locus_tag="PK288_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18718.1"
                     /translation="MRTENKVGLRFKPLPGNFQVWDCEFENISSDTVTISNVLPFEID
                     DESVSITGYGTDELARACLFRPGCRPLRIILPDNAWELGYTSFGIDDEYSVCSMTRRR
                     NITGGIRLRYATKLPPGAKVQYRIYFEAFKGTWQDGLSVMFREKYLYDTENFDNSLYE
                     RPDLKWIRESYLVVLQMAWDREFFDRISGKYTYPDVLRNGMSRFGHIDVFGIWPTWPR
                     LGLDQRNQWDLYRDLPGGIAQLRNFVRLSHQSGTRFFIAYNPWDLSTRKEDHLRGLAR
                     VLKETEADGVVLDTRSSSSAELQLAADSVGRGIVMYSEGMAV"
BASE COUNT         3723 a         3008 c         3393 g         3956 t
ORIGIN      
        1 aaaacggtgg tattgcttat taatgatccg ggatatgtca cttccgacag cagtcttttc
       61 aacggcaggg cgatgacata ttatggacgc tggacatata aatacgagga agctgcgcgg
      121 cagggagccg aagcagtaat tatcatccac gaaacaggtg ccgccgctta cccctggggt
      181 gtggttcaga acagctggag tggccctgaa ttctgcctcg aagatgatga attggcttca
      241 tccggcctgc agttcaaagg ctggatcaca aatgaatcgg caaggaaaat attcggatct
      301 gccggccttg attttgaccg tttgacaaaa tccgcatcca ccaggggctt caagcctgtt
      361 gatatgaaac tgaaactatc cctggagctg agaaacagga ctgaatatac ccggtcaaac
      421 aatgtcgctg ccgtctggcc gggcagcagg aggccggatg aatacatcat ctacactgct
      481 cactgggatc attttggtgt aaacaacgct tttaagggcg attccatact gaacggtgcg
      541 gttgacaacg caaccggaac agcatctctg ctggtgatgg caaaggcatt cacattgctt
      601 cacccggtgc aggaccggtc agttctgttc ctctcggtta cctgcgagga acagggattg
      661 ctcggaagtg attactatgc aagaaacccg cttttccctc ctgggaaaac gatcggggtg
      721 ataaacattg acggcctgaa catctttgga aggacgaagg acatgaatat taccggttac
      781 ggcttcagtg gactggacga ttatgcaatt gcagtcctgg gaaaatatgg ccgttatgct
      841 actcctgatc ccacccctga gaaaggcagt tatttccgat ccgatcattt tagttttgca
      901 aaggtgggaa ttccttcatt gaacctttcg tcgggggtcg acaatgttga gcatggcagg
      961 gaatggggac tggcccagaa cgaaaaatgg accatggata attatcacaa gccttctgat
     1021 aattacgagc cagacaaatg ggatttcagc gggatgattg aagatacacg tgtatatttt
     1081 gagattggct atgatctcag caacactgat gacttcccgg agtggaagcc gggaatcccg
     1141 tacaagtcac tcagggatat gatgatgcag aagcagaact gatgcatatg agaatacagg
     1201 gccggtaatc agggtattcc ggtaattaaa tccctttctt tccgtagatt cttgtttgtt
     1261 aacgtgaaaa agatgttcta tagttaatga agtcggctga tctggttttt tcatttcaga
     1321 gagcggtaat catgtgcaac ggaaagctcc ttgcaggcga tctgagcttt gatgtgcacc
     1381 gcggtgaaag ctggtgggtt tgcggggcca acggaactgg aaaaaccacc ttactcgaga
     1441 tcattgccgg gaagcagcgg ctggccgggg gtgaaatgat cctgccggaa aatgttaccg
     1501 tttcggaatt ctattcatcg gttgccattg tcaggaggga cttttcattg tatcaatggt
     1561 ttaccggttc ggccagtttt taccagcaac gctatttttc cccgggagtt gaagagactc
     1621 ccggggtttt ggacttgatt catgagaaaa cagggttttc aaaagaagat atttgttcag
     1681 ccgctcatga atttggtttt ggttcgctgc ttgacagacg cattgtttca ttgtcgacag
     1741 gagaaggacg gagaatcttg ctgttaatgt tatggctgag cggcaaaaag gttatttgca
     1801 tcgatgatcc ttacgaaggc ctggatcctg aaggtaagca acttgttacg gatacccttc
     1861 attccctggt taaaaagaag gcagctgtcc tggtaactgg tacagatacc agtccgccag
     1921 actttgtaag tcacgtttta tatctccgga atcagcgggt tggatatgcg ggggaggcgg
     1981 agctttttaa tttgcggaac gctgattctc cgcagcctta taagccgccc ggactgaaaa
     2041 gatatgttcc tgacaatttc aatcgtgcgt ttatgacaat ggctgaaatg aaaaatatta
     2101 ccatcaggta tggcagcaac attgtccagg aaaatttctc atggagaatc agaaggggtg
     2161 aaaaatggtt gctttcaggc cccaacggat cgggaaaaac tacgctgatg agtcttatct
     2221 atgctgataa tcccatggct tatgcctatg atctcgttct ttttgacaga aagaggggaa
     2281 cgggggaaac catatgggaa attaaggaac ccattggata cttctcatcg gaactccaga
     2341 atttttttcc gcgtcatctg acgcttcatg aatcagtttt aaccggatac agcaaccatc
     2401 ttctggttag gaatgatctt acaccggaac attaccggca agccgacgga ttgattgagg
     2461 cagccggttt gtcggctttg aggatggtgc ctatgtatcg cctttcattc agtcagtgcc
     2521 gtcttgcact ggtttgccgg gcgctggtaa aacttccccc tgtggttatc ctcgatgaac
     2581 cctgccaggg tcttgaccgg actgcggcac agactgtcaa ctgcctggtg gacaatgttt
     2641 gcagcggaga actcaacacc ctgatctatg taacccacag aattgaattt gttcccggaa
     2701 taatcaataa gcatttgcgc cttgttaaat acagtcagga ataagttaat catttctcat
     2761 cggaacgttt ggccgttacg ttttctatca ctgcaaatac cggccgggcc ctgaagcact
     2821 gttaattaat taacagtatg cggattgttt tgattcctgg cttcagggca atttgctgag
     2881 tttgttaatt gcgcttatcc ggggctgggc gggaggtatc acttcgggat atccgagagc
     2941 caggatccct gcgatttttc cctcttccgg gatcccgaat aatgaccggg cctgaggtgt
     3001 gcttccgaat gagagccata cgccggctat ctgtttttcc catgcagcaa ggagaatgtt
     3061 ctggatagca gctgcagccg acatcggaaa gtcatatctg tcgagatcgg taagtgcggg
     3121 agcacaggtt actgccagta taaccggtgc atcgccgaag ttcatggaga aattgcggat
     3181 aacctgcttg cgctcttcag gtaagtcggg attaagcaac ggccctgact ccatggcctt
     3241 cctgtatgcc actgccagtt tgccacgcgt ttccgggcct gcaaggatga attcccatgg
     3301 ctgattgttc ccatgcgatg gcgcccaggt accggcttca agaatatcca gcaacgtttc
     3361 gtctgatata gctttggagt tgaatctgcg cactgtcctc ctgttaagga ttgcttcttt
     3421 aagttccatt gtttcctttg aaattatttg attggttata tcgggatcat tgatcttgtc
     3481 gggaaaaacc gatgcctctt ttcattttgc aaggtctata gggtctgggt aatcgtatcg
     3541 ggctgtattt cgaacatggc cctggcccat ggaaaccttt gcttcaattc gttgtattca
     3601 tcgccttccg agataaaagc cagtgtaccg tggatgagga atcctgtccc cttggaatgg
     3661 aatccctgaa cctccctgct tccgagggtc attacgatct tattgttttt tcggacaata
     3721 gcttctgtag ttctcatgcc gccggccgga ccgataattt ttccgccggt ggtaaccttc
     3781 aggtaactgt tccatgtatt taccatatgg atgccgttat cgtcaagcat tgcaatggct
     3841 acaaccccct catgcttcag tacctccagc agcttctcag gtatcagggt tttcatgtat
     3901 gaagtatgtg tgaaaaatta aaaatacaat gaattttatg tcacattatt cctgaagttg
     3961 tttttatcat aaacttgctg cgacatgaga gctcccgcaa tattggattt cgtttttatt
     4021 aattgaccat tactgctgtt ttcctgtttt taatcaaatg attttatgta tgtttatatc
     4081 aaactaacct tatactgaaa tgaaaaaacc gttccagaaa catgcaacaa gtcttttcat
     4141 ggctttgata ttgatgctct ttggttttcc ggctgtttcc cagttaaatg aagcttcccc
     4201 cggagcagcg ccgtataaaa atccttcagt gcctgtcatg ctgagggtgg aggatcttct
     4261 ttcccgcatg acactcgcgg agaagatcgg gcagttaaat atgccttgtg tatatgaatc
     4321 tgctctggga aatgatattg aatcccggac cgttaactgt aaaacgcttg ctgagggtac
     4381 ttttcaggac tatctcggtc ccataggagg ttttttcacc ctggcgaaca cgatattgca
     4441 tgaaggcaca cttcagcagg caaatttctt caatgagctt cagaagatag cggttgaaaa
     4501 aacacggctt ggcattccgc tcctgcagac agaagaggga acacacgggc tgatgtgttc
     4561 gggaggaacc atctttcctg agggactggc aattggcagt acctggaaca tggatctggt
     4621 cagcaggatg tatacggtag cagcctgtga ggcaaggtct gtggggattc accagttgtt
     4681 caccctggtt gttgaaccca acagggatcc aaggcttggc cggaatcagg aaggattcag
     4741 tgaagatccg tttttctgtt cacgtatggc cgagacaata gtcaatgcag tgcagggaac
     4801 agatgtaagc gcaaatgaca aggtcgtggc cggcctgtgt cattatccgg gccagagtca
     4861 gccaaacagc ggaatggaaa gaggagcgat ggagatttcg gaaaggcagt tgcgggaagt
     4921 ttttcttcct ccatggatcg ccggggtaaa aaaagccgga gctcttggag tgatggccac
     4981 ttacccggca gttgacggga tcccgaccca tgcatctgaa ttcctgctga caaagatact
     5041 cagaaatgag ctagggttta aaggattggt actgagcgaa ggaggcggac ttaacacact
     5101 gttgtacacg ggtgttgccg agaacgatca gcaggccggt gaactagcgc tcagggcggg
     5161 acttgacgtt ggaatttcat tcgaagacgg ttatcttaaa ccgctgatcg acgccgttaa
     5221 ggaagggaga gtgtcaatgg agctgattga cagatctgta agacgggtac ttgaacttaa
     5281 gttcaggctt gggttattcg acaaacctta tgttgatcct caaagggctt tggatgttac
     5341 ccataccaaa gaaagccgtg aacttgccct tgatgctgcc cgggaaggaa ttgttctcct
     5401 gaagaatgag aaaaaccttc tgcctgtcag aaagaatatc aggaaagtag cggtcatcgg
     5461 acccaatgcc gacaatgaaa gaaatcagct tggtgattac gcggcacatg ttattctcca
     5521 ggagataaca acggtactag acggaataag atcaatggtg tctcccggaa ctgttgtcga
     5581 gtatgtaaaa ggatgtgatg ttaccggtaa taaagtgaat gaactggcac gggcccgcag
     5641 agctgcctcc ggtgccgacc tggctgttgt tgttctggga gaaaatgaat ggcaggcccc
     5701 tgacaagacc ggtacctcag gggagggata tgatgtggcc agcctcgatc tgacagggct
     5761 gcaggaagat cttctgagag ccgtgcactc atccggaact ccggttgtac tggtactcat
     5821 taacggcagg ccgctttccg tccgctgggc agcggaaaac atcccggcca ttattgaggc
     5881 ctggatcccc ggtgaagcgg gtgggacagc tgttgcagag atcattttcg gcgactgcaa
     5941 tcccagcggt aaactggcgg tcaccatacc caggcatgtc gggcagatac ccttctatta
     6001 caattatatg cccgaaaagc tttactggat ggaaaaagga tggggaaaag cctatgccga
     6061 catgcctgca acaccattat gggaattcgg atttggattg agctatacta cattcatgta
     6121 cagcaacctg aaaattaacc ctgaagttac cgggatcttt ggtgaagtga aagtgagcct
     6181 ggatgtggaa aacaccggca agagggcggg cagggaaacg gtgcagctgt atgtcaggga
     6241 caggcatgca tctgttacag tacctgtgaa ggaactgaag ggattcgaga agataagcct
     6301 tgcaccgggt gaaaaaaaga cagttgaatt caggcttacc catgatgacc tggctttgta
     6361 taacaggaac atggagtttg ttaccgaacc cggaatattc gaggtcatgg ctggcagctc
     6421 ctcagccgat atcagactga aaggaaagtt tgagctgaaa aactaggtaa tcatcgggaa
     6481 tgaggaatac tgcatattca ttctgctgaa tagctgtttc cctgtatttt tactgacttt
     6541 accagggctt tacccgaaat gaaactgtaa tttgttccca tgtgttcaag ggtgacatca
     6601 ctgccattga tctccagtgt aatctgattg cttccgtccc tgtgaacaat gagatctgca
     6661 agcagaagag ctttttcaat gccgtcgccc ctctggaaat tccagacttc atcgggaagg
     6721 gcaagccttt tttcatcata tattgattca ttttcaagat ccatgagaat cccgtaaacc
     6781 tcattcaagt ccttctcctc aagatccttg aaacataccg ggtttctttc aatggctgcc
     6841 ttgatgaacg gtacccagtc aatcatgtcc atctgccggt aaacataaag cgaaagcagc
     6901 gctgtttccg aaatgcctgc catatcattg atctttttta agatgctttc gcgtgtgtca
     6961 tcaattccaa tctctggttc gaggcttttt ctgaatgact tggccggatc gggcagcctc
     7021 ggatcggtaa caataaaaac cctgaggtct gaaaacattt cgtcaagcag cgaactgggc
     7081 actgagccgg ccagcttctc aaattcctta atgaattcat caatgcttat gctcttgttc
     7141 tcctggaaga ttttttccac attgttcagg agaatttttc cgtttatggg actcagatga
     7201 aattcatcgc cttctatctc atttacaagc tgctcacgtg tttcttttgt taaattgtgt
     7261 ttgcttgtgt gttcatattc gaacatcttt tcaagactta tgtataagtg cttccctgaa
     7321 cagtcgcaat aatactggaa aagagatttg tacctgggct tgaaccgaag gaaattaatg
     7381 aatattgtgt cagtcagctc agtgcgaagg aaacttttaa gttttgtgct gaaagtttcg
     7441 taatcttttc tgcttattgt cgcctcattg taaataatgt gaatgaatcc tgttatatgg
     7501 gtgaccattg tgattttttc attttccagg gcacgtctgg ctttctgtga caatgaggtc
     7561 ccgttaaacc acatgttctt tgtaacaatt ctcctgttgt ttgtgataag cccttccttt
     7621 tcagtgacga aattctgaga atgcagggga gttgctataa ggaatatttt ttccagcggg
     7681 atcctgccga caataaacaa tgctgccgca taaagcgacg atagtgaaac acattccccc
     7741 gctccggtgg taaacccgtc cttgtacctg aatgcggtca ttgcttccac cgtttcggtc
     7801 ttccagtacg gaattacttc cgaatcatat ttatccagcc cgaagtgctt cctgatatca
     7861 atatcgaaat aatacttatc cccctcgtat ccgaatagtg catttgattg tttcatgatc
     7921 tcaaggtcaa agaaatcact gaaccgggac atttccctgg tttttgtagt gagtccccag
     7981 gttgacagat cgagattgaa aacataattg tcaataataa actgttttat ccggttcacc
     8041 ttgtaattga aactttttga ttcaatattg ctgaaccatg gatcatccct ccatttccag
     8101 ataaccggcg acatgatatt ggcgattaca agcggaaaaa agagttccgg aaaaataaaa
     8161 atctccatat cggagagtgt aaatgccgaa gaatattttt caagcttttt aatttctgta
     8221 acggatggat tattatcagg caccataata aaatgatcaa atcaggaatc ttgacattaa
     8281 aataacaatc gttttcccgg aatgttttgg aaaaatatat gaacggaagt acaggacaga
     8341 acggagcccg tgaatttaaa ggcatctttc ggcttccgaa aagaagaagt tggattcacg
     8401 gagggcattt tcatcactgt ctgagccatg aactgcattc atctgcactg aggatgcaaa
     8461 tcttttcctg acggttcccg gctcagcttt tgccggatct gttgctccga tcagcttcct
     8521 gaatttatca accgcatttt cctgcctgag tatcatgaca atcacaggtc cgcttatcat
     8581 gaattccaca agatcgttat aaaatggcct gtccttatgt atggcataga agctcttggc
     8641 ctgctccctg gtcatcctca gaagcttaag tgcaactatt tcaaagcctg cttcatgaat
     8701 cattgccagg atcgggcccg tgtttccgct gccgaatgca tcgggtttaa ttattgagaa
     8761 tgtgatttcg ccgttcattg ctgttattta tagagtcttt caagtcaact gtcataagga
     8821 aatttcctat ctttgacaac ttgtcaaaaa aatggaaaaa gtgattgatc cggccaaata
     8881 tacaaaagaa ttatcgaaac tgattgcatc gtctgacaac atacttatta tctgccacat
     8941 taatcctgac ggagatgcaa ttggctcaca gcttgcattg ttccactatc ttacatcaaa
     9001 aggcaggcat gttgacatga tctctccaaa taatcttcag gagtttctga aatggatgcc
     9061 gggagctgac cagataagga tattcataag acagagagct gaatgtatag ccctgatcaa
     9121 acaggccgat ctgattatta tgactgattt cagccatccg ggcaggctgg gtgaggcaga
     9181 aaaatacgtc atcagctcag gggcaaaaaa ggtaattatt gaccatcatc ttgatccggc
     9241 tgattttact gatctcacga tatctgaccc atccaaatgt gctacgaccg aactggttta
     9301 tgaactgatt accctgatgg acggggtacc atatacggat aataaatttt ccgaatgcat
     9361 ttatgtcggg atcatttccg acacgggaaa ttttgatcat ggtgcattga cgggcaggac
     9421 cttcaggatc gttgctgacc ttctggataa cggcatcaac agggagaaga tgattaatgt
     9481 tgttttcaac aattattcgg ctgaccgtat gcgcctcatg ggacttgcgc tgaataagcg
     9541 catggtgatc attcccgaat taaagacggc atatatctac cttacccgtc gtgatctgac
     9601 cgacttcaat catgtcaagg gcgatactga aggatttgtg aatcttcctc tttcaatcag
     9661 ggatatagta ttctctgttc ttttcattga aaaggaaaat cacgtgaaaa tttccttccg
     9721 ctcaaaaggg aatttcccgg taaataaatt tgcttcagag tatttctccg gcggcggaca
     9781 catgaatgcc tccggcggag aatattacga gaatcttgat aatactatcg cttatttcct
     9841 ccgtatcctg aacgacaatg ccgccctgat aaaaaatgca accgtttaaa tattaccgga
     9901 tctgtatctt cacttttttt ctgtcagtcc tgctcatctc ctgcaggggc aggaacgagg
     9961 cagaaagagt tgtgagcggg ccgggaaaga acgacatgac ggaactgaac cgttatttcg
    10021 tccagaaaga cagggaaatt attgaaaatt acattgaaag gaaaggcctc ggtatgcagg
    10081 aatcccctac gggtctctgg tattttttaa gaaaggaggg aaggggaaat ctgatatctg
    10141 acaataaaaa ggtaaggatc gcatacgaat gttccctcct ggatggtaca aaatgttatt
    10201 catcagatga ttcggggccg aaagagattg tccttgggaa aaccggcatc gagcagggac
    10261 tttatgaagg tcttaaaatg ctgaggcctg gggcagaagc catatttatt atccctccgt
    10321 ttcttggtca cggactgata ggggatgggc ggaaaatccc accgcgcgcc gtaattgttt
    10381 atgaagttaa ggtgctttca gttgaataga tgtgtatttt attcatttgt agcctgaaat
    10441 agtttccggc agctgcagct cgacactctg gtctgttaat gatttcacac tgccggagga
    10501 ccattgaatc gataaatcag gaccggaact tttactgaag aataaagata gagggatgat
    10561 tcttaatgtc cggaatattt ttcctccatt caccgatagt ctttgttttt atgctttccg
    10621 aatgcaggga tatatcgctt gcaatacaaa gcttcctgtc agagctgcaa acatccagca
    10681 gggattccag gattttctgg tttctgtaag gtgtctccat gaagatttgg gcctgaccgc
    10741 tgcgtgattt tgtttcaagt tcacggattt ttgcgatcct ttctttatcc ctgaccggca
    10801 aataaccgtg aaacgcaaaa ttctgcccat tcagtcccga cgccattaaa gccatggtga
    10861 tgcttgatgg gcctgaaagg ggaattaccc ttatcttttt ctgatgagcc agtgatacca
    10921 cccgggcccc gggatcagct attcccggca taccagcctc actcatgagc ccggcatccc
    10981 tgccgtcaat gacaggatcg aggcaccgga taatatccgg ttcgggtgtg tgctcattaa
    11041 gttcgacgaa caatgtatcg ccgatcggga attcagtgtc gatcagcctc agaaatcttc
    11101 tggcggtcct gacgttctca acaatgaaaa ccctaaggga tctggtaatt ccgattactt
    11161 tgtcaggtat gagtaacctg taatcatcac ttcccagagt gaccgggatc atatatattt
    11221 ttcctggcac agcagagctt ttggaatgta aatttaagga ttatgacgta atgacccgct
    11281 taaataattt tattccggtt tcgtctgttg cgccatttca ggtaatacct tccattcagt
    11341 tgtattgtac ccggatggat ctgtcttccc gacatgatgt ttccctgaga cgactttaat
    11401 tatctggtat tcagtaattt ttctgaagta ttcgtataat acacctatgg ataaaaccag
    11461 cgattggaat gaaatcctgg aaatgaacaa ctttccgata tgactgccgc gcccgatatt
    11521 aattgagcat tccattcatg caacggatgg agatgcttta cccagaccgc tttcatcccg
    11581 ttatgttcct ggctttccgg ctctgcagga aaactgatcg ggtaagaaaa gatatccggg
    11641 aaagcggtct gtttcaaatt tacaggactt attgcctgtt agggaatttt aatataggtt
    11701 tgccttcaaa ttcgttacct gtgaaaatta catttctagg aaccggaact tcccagggtg
    11761 ttccggtcat tgcttgtgaa tgtgatactt gtacatcgga tgatattcat gataaacggc
    11821 tgcgcacatc gatacttgtt gagaaagacg agacctgcat tgtgattgat gccggccctg
    11881 atttcaggca gcagatgctg cgtgaaaatg taaagaaact cgatgcgata cttcttactc
    11941 atgaacacaa ggatcatatt gcaggtatgg atgatgtaag ggcttataat tacagaagcc
    12001 gtgatgcaat tgatatttac gcggaagaga gggtgcagaa ggctgtcagg aaggaatatt
    12061 cctatgtttt tgccgagtat caatatccgg gaattccgaa gatgcggctc aaccttattc
    12121 ctgaatatgg atttgaggtg ggttccattg gtgtcctgcc tctcagggtt tatcattatc
    12181 ggctgccggt ttacgggttc cgcataggca attttgcata tattactgat gcaaattatg
    12241 ttccggaaga aacgaaggaa aagatttttg gggtcaaata cctggtaata aatgcactgc
    12301 gaaaagaaaa acatatctcg catttcagcc ttcgcgaagc aatcgatttt ataaggcaga
    12361 tatcaccgag aaaggcattc attacccata tcggtcatca gatgggacgt catgatgagg
    12421 tatccgccgg gctgccggca ggaatcatgc ttgcttatga cggactcagg ctggtatgtg
    12481 atgaataatt gcttaagtcc cctattttct tctgaagttc ttgttaagct ttttctggta
    12541 cgacggggaa taataatacc tgtttcttcc catcggagtg gtactggcct cctgggtggt
    12601 catctttttg gaatgggccg gagcccttct cgcaggcata catcccgtat cggctaacaa
    12661 aataagcagc atggccggca atattagtgt gattattttc ttgaatgctg acatcttttg
    12721 taatgtctgt cggtttgtac ggagcttttt aaccaatgaa ttctgcatgt aactgcggca
    12781 cttgcgaatt ccttaactta aaaacttttt tttagtgact gcgatattat aacttgcata
    12841 aaaatcaacc ggttattatc attagttttc ctatgatccc tttcgtatta aagatcagat
    12901 atttactact ttttttcatc tgccttattc ctgtttccct gaactcccag aaacttgaaa
    12961 aggttaatat ctcaaccgtt tcaggcctct gtatagacag tattacatta actgacaaca
    13021 ataatcttgt aattaaacgg aaaaaacctt ttttaagttt ccagattgat caaaaaaact
    13081 ataacacagg aacgcagggc gttattgctg aagattccgt tattcacgtg agaacagaga
    13141 ataaggtcgg cctgagattt aaaccattgc cggggaattt tcaggtatgg gactgcgaat
    13201 ttgaaaatat atccagtgac acggttacca taagcaatgt attgccattt gaaattgacg
    13261 atgagtcagt cagtattacc ggttacggga cagacgaact ggcacgggcc tgcctcttcc
    13321 ggccgggatg ccggcctctg aggatcattc tgcccgacaa tgcatgggag ctgggctata
    13381 catcgttcgg tattgatgac gaatattcgg tatgcagcat gacacggcgc agaaatatta
    13441 ccggagggat caggctcagg tacgccacaa aactgcctcc gggagccaaa gttcagtaca
    13501 ggatttattt tgaggctttc aagggtacct ggcaggatgg attgtcagta atgttcaggg
    13561 aaaaatacct gtatgacact gagaacttcg acaattccct ttatgaaaga ccggatctga
    13621 agtggataag ggaatcctat ctggtcgttc ttcaaatggc ctgggacagg gaattctttg
    13681 acaggatcag cggtaaatat acttaccctg atgttttgag aaacggcatg agtcgttttg
    13741 gtcatattga tgtatttgga atctggccaa catggccacg attaggtctt gatcagagga
    13801 atcaatggga tctttacagg gacctgccgg gagggattgc ccagctcaga aattttgtcc
    13861 gcttgtcaca tcagagcggt accaggtttt tcattgcata taatccctgg gatcttagca
    13921 cacggaagga agatcatttg agaggattgg cacgagtact gaaggagacc gaagccgatg
    13981 gcgtggtgct tgatacaagg agcagctcaa gtgccgaact tcagttggct gccgacagcg
    14041 tggggagagg tattgttatg tactcagagg gcatggctgt
//
LOCUS       DAOOYG010000066        14021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4406_length_14021_cov_7.026923, whole genome shotgun sequence.
ACCESSION   DAOOYG010000066 DAOOYG010000000
VERSION     DAOOYG010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14021
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4406_length_14021_cov_7.026923"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PK288_07060"
     CDS             <1..837
                     /locus_tag="PK288_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18719.1"
                     /translation="HPAPGFLPVSSCSVELNDTVYNLKLVRSLGLDERPEVKLHIYDP
                     DIRARLFYRRYRSEEPYMTADFVYKAYPVNSFLMNRVFGITEEKGLFAEIPSQPPAGK
                     IEYYIEITDSKGTTALFRENPVVIRFKGAVPGSILVPHILFMFLAMFFSTLTGLMAAV
                     NHPKFRKYGVITLVILGIGGMIFGPLVQKFAFGDLWTGIPFGWDLTDNKTLIAFIFWI
                     AAVWLNRRKERPVYSIIASVVLILIFSIPHSMFGSELDYESGKVIQGSILAFFLSLRK
                     KN"
     gene            943..1503
                     /locus_tag="PK288_07065"
     CDS             943..1503
                     /locus_tag="PK288_07065"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM18720.1"
                     /translation="MANAEAAFRNIHQDLVDGCKTGDQKAQFQIYKLYYKAMYNTSLR
                     IINDTMEAEDIMQESFLSAFEKIDTYSGTVSFGAWLKKIVINRSLDALSRRKAIFEDI
                     EAHTGIRDEGSDDVSRQEEIDIKVDEIKEAINNLPDGYRIILSLYLLEGYDHDEIAEI
                     LGISSSTSRSQLSRAKQKLISELKNY"
     gene            1516..2079
                     /locus_tag="PK288_07070"
     CDS             1516..2079
                     /locus_tag="PK288_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18721.1"
                     /translation="MKNIEDIIRNNRDLFDTAEPQDGHFERFCSRLEERFPAHTMVRR
                     SIVPYLLKAAVVTLLVTLSSLWTWEHFLKPGRDRMTLGDVSPQYREVENYYMYQVNLM
                     SNEIASLDENDEGQKQMLLAEMKSMDSVYIQLQKDLRANPNDERIINAMIEHYQTKLE
                     VMNYIVNQLKIIRNDNQTRKAHENSGL"
     gene            2036..3142
                     /locus_tag="PK288_07075"
     CDS             2036..3142
                     /locus_tag="PK288_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18722.1"
                     /translation="MITKQGRLMKTQAYKSGIILVLFILLTSGAIRGQQAVKDFHKEY
                     TAGPGTTLDVSNRYGNVTVETTDGNMVIIDVRVTVDLPSRERAEKLLSYIDVNFAESD
                     NVITAKTVIDDKFSFSGWGGSNRKFSINYKIKMPARINFTLANKYGNTDLDEIHGLVN
                     LDIKYGNLTAENLSRGNEKPLNALTIAYGNASISSASWLDINSRYVGNLRIDRIQALL
                     ISSKYSKIRLGETSSVVGDSKYDNFRISKINNLVLDAGYSDIVVETLLKKLKFEGGYG
                     ALTVGEIPAGFELIDTDTRYIGVKLGIDDNASYKLDARLSYGNLKFNEENYRNQKRIA
                     QSTSNEIQGIVGNETDPTSVVKVHASYGSVRLTR"
     gene            complement(3245..5719)
                     /locus_tag="PK288_07080"
     CDS             complement(3245..5719)
                     /locus_tag="PK288_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPM18723.1"
                     /translation="MIYPGNFEAKTGFDRIRALLKDNCLSTMGSELIEEMKFMTDLQP
                     VDARLSETFEFQQLLLLEDSFPSEDYFEISGCLNKIRIEGTFPETREVFELRRSLETV
                     RSILAFFRRKESDKYPVLRSLCGNVKVFPYVIDAADRIIDRKGEIKDSASPRLKEIRG
                     SISSLTAQASKRLNAILRQAKAEGIVEADTVATVRDGRGVIPVNASEKRKIRGLILDQ
                     SASGKTVYVEPVEVVEINNEIVELEYEELREIVRILTSFADDIRPYIDDLLLSNDFLG
                     QMDFIRAKALFGNQLGSIKPALADVPGISWKRAVHPLLELAFRKTPGRKVVPLDIRLD
                     RNDRILLISGPNAGGKSVCLKTVGLLQYMLQCGLTIPVGEGSESGIFKNIFIDIGDEQ
                     SIDNDLSTYSSHLLNMKFFVRNASSETLILIDEFGTGTEPMLGGSIAEAVLGELNRAG
                     VYGVLTTHYTNLKHFASLTEGIVNGAMLFDNHLMQPLFQLSIGRPGSSFAFEIAKKIG
                     LPEHVLAIASEKAGVKNINYDRHLKDIARDKRYWEAKRQNIRHQEKRLEELIAEYEKE
                     LAGAKNLRKEIISRARDDARALLDGSNRIIENTIRQIKEAQAEREKTRDLRQKLDEFR
                     ENIEEEPKLVITDAEEKATRLAQKAKKIKPEAVRKKAEPMAEKVKPLSAGDAVRMVET
                     QAAGEIVKIESGMATVEAGSIRFLVPLEKIERISRSELKKSERSRQAVHRSDPVIEQR
                     RSSFRPEIDIRGVRGEEALNIVRDFIDDALLVQHRNLRILHGKGNGILRQLVRDYLAT
                     VNVVRSFRDEHVEMGGSGITVVEMDF"
     gene            5936..7510
                     /locus_tag="PK288_07085"
     CDS             5936..7510
                     /locus_tag="PK288_07085"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPM18724.1"
                     /translation="MKSLNYLATLIVLIFFPVLVNAQTETVDLNMIYRIKQEGMKNSA
                     IEELAFGLTDLSGSRLTGSPGLARGNEWAMKKMLELGFQNVRIEEAADFSQGGWDNLK
                     TYAAMISPYYINFACNPVAWTGSTNGPVKAEVILLDVKTEEDLDKYKGKLSGKIVMMP
                     STASYEVSFEPLATRYTDDELKELSMAAVRPQGGRVPADWANYMAQRALQMKITGFLK
                     SEGVAVILNNSGFFNVPRSASASYKAGDPEPVPQLSIPVEAYGRMERLIAHSVRVEME
                     IEIINKFLGPQKVYNVIGEIPGTDKLLKNEVVLLGAHIDSWHGGTGAADNASGCVVMM
                     EALRILKNLDVAPRRTIRVALWGGEEQGLIGSRAYADKYLADRETKAHKPDFDRFAAY
                     FNMDNGTGKYRGIYTQENDMVRPIFEEWLKPFADMGAATVSGRNTGGTDHLSFDAYGL
                     PAFQFIQDKIEYGRGYHSVMDTYERLLMNDLRHNAIITAAFAYNAAMRNSKLPSKPAM
                     KPATNVKQPQMAPWMN"
     gene            complement(7720..9882)
                     /locus_tag="PK288_07090"
     CDS             complement(7720..9882)
                     /locus_tag="PK288_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPM18725.1"
                     /translation="MGTQKNISLVAEKLGLHLWQVENTIRLLEDGSTIPFISRYRKEM
                     TGSLDEVKLLQIRDEYNRLKELDIRRESIIRSIEEQDKMTPELRQKLEAATRMSEIED
                     LYLPFRPKRRTRATIAKEKGLEPLASLIMEQGETDPGKKAEEFLGDSVADKGEALAGA
                     CDIIAEWISEDQKARARLRILFEREAVISSKVVRGKEQEGIKYSDYFDWSEPLRKCPS
                     HRLLAMRRGEEEGFLRLSVLPEDEKAIDILKSLFIKGRNESSRLVGEALADSWKRLLS
                     GSLETEFRNVSKEKADGEAIAVFAENLRQLLLASPLGEKNVLAIDPGFRTGCKIVCLD
                     RQGNLVHNETIFPHPPQNQTALSVRKLLTLVNAYKTEAIAIGNGTASRETEQFIKWIK
                     FEKDIQVFVVNEAGASVYSASKVAREEFPDYDVTVRGAVSIGRRLMDPLAELVKIDPK
                     SIGVGQYQHDVDQQKLQQSLDEVVVSCVNAVGVEVNTASSHLLTYVSGLGPQLARNIV
                     DYRTENGPFRSRNEFLNVRRMGAKAFEQCAGFLRIRNAGNPLDSSAVHPESYHVVEKM
                     SADLGCDVRELMADQEKRKQIRLENYVTETTGLPTLRDITEELAKPGRDPRSKIKEFS
                     FADIHTMEDLTPGMIVPGIVTNITKFGAFVDIGIKQDGLVHISNLSSTYVRDPADVVK
                     LHQHVMVKVLDIDPERKRIQLSMKGLDKGNKGQEPSSF"
     gene            complement(10025..10612)
                     /locus_tag="PK288_07095"
     CDS             complement(10025..10612)
                     /locus_tag="PK288_07095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPM18726.1"
                     /translation="MLRNNYPATISAEEIALKELSWFRGEIVLIDNMAAFRKTFPRLI
                     GSPFLGFDTETKPSFRKGRKNRVSLLQLANSELACLFRINRIGIPDPLTELLASPDVI
                     KAGVAVHDDISFLKRINGFVPSGFIDLQKFVKEYGIQNSGLKKLAAIVLGIRISKRQQ
                     VSDWEADELTEAQIIYAATDAWICHQIYDRLTKKG"
     gene            complement(10599..11222)
                     /locus_tag="PK288_07100"
     CDS             complement(10599..11222)
                     /locus_tag="PK288_07100"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HPM18727.1"
                     /translation="MNKKSDPVYSRSVVEFVAAANEFCKYAEHGSEVPGDELLRIFQR
                     LLPYLYIKASLLPAPEPVFEDGNEKFVTEADWNRVHDTLKDRFGAADDYYEVFDERIA
                     DGGEPVIASLSENFADIYQDLKDFLLLYQTGSKEVMNDAVWECRMNFENIWGQKLVNS
                     LRAIHRFIYSGESTDIAENGGSDSYERDKSEWFISRRQKEFREDAEE"
     gene            11425..12840
                     /locus_tag="PK288_07105"
     CDS             11425..12840
                     /locus_tag="PK288_07105"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HPM18728.1"
                     /translation="MKKFLIVFTALLLIHLTSSHFLNGQPAGLTRGWRAGVAKVNITP
                     EGSIWLAGYASRDHPSEGVLHDIWSKVLVIEDSLGNRAALITNDLLEIPIHYSTQIRD
                     RLEKEYGLHRSQVILNCSHTHSGPVMRDTLANWYPLDDAMEKKVKKYSEDFVEKIVEM
                     VGRAIYRMIPVEISAGNGTARFQVNRRNNSEAMSEYYTDLKGPNDYAVPVLRIDRKQG
                     GIMAIVFGYACHSTVLNFYQISGDYPGFAQIELENLYPGTTALFFQGAGADQNPLPRR
                     SVPLARQYGQTLSAAVDRVLNEKMKRLVPLLKTAYSEIPLQFDGSPPDRQELLGHIHD
                     TSSTAPYLKLMAQTLIRRLDNGEVFRNGYPYPCQVWKLGDQIIMALGGELVVEYALEL
                     KKRFGQDVFVMGYTNDVMAYIPSDTILSEGGYEGTRSVFFTRAWSRNTQTAIINEMSS
                     LAGKLGITPIEAPDHNETAGR"
     gene            12901..>14021
                     /locus_tag="PK288_07110"
     CDS             12901..>14021
                     /locus_tag="PK288_07110"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM18729.1"
                     /translation="MTKRILTRRRFIASVSAGSVAAIAGSVPVLGKTGNDAGRLAILG
                     GIPAIPPKKWPDWPYVDQKMIDAMIRTTKSGIWSRIQSETGSVPTFEKEFAALMGAKR
                     CVTTGSGTQALSCCVEAMGIGAGDEVITSPYTDMGTISSILTSRALPVMADLDPASFQ
                     IDPADVERKITPATKAIMPVDMGGQPCNMARINAIAKKHKLRVIEDSCQGHLGEYQGK
                     KLGTVGELGCFSFQTSKTIACGEGGAVIGNDEALMDRVFSVMMNGSHRIIGTKYRMTE
                     FEAALLLSQLEGAKERFAIRNRNAAYLTSKLRKFPGLVPQKLYPGTTSGSYYLYMMAY
                     KKEYFNNADRSKFLKALAAEGIELSPYIAKGLHREPWTE"
BASE COUNT         3781 a         3492 c         3210 g         3538 t
ORIGIN      
        1 catccagccc caggcttctt accagtttca agttgtagcg tcgaactgaa tgacactgtt
       61 tacaacctga aactggtaag aagcctgggg ctggatgaac ggccggaagt taaattacat
      121 atatatgatc cggacatcag ggcaaggctg ttttacagga ggtacaggag tgaagaacca
      181 tacatgacgg cagattttgt gtacaaagca tacccggtca attcatttct tatgaacagg
      241 gtcttcggga tcactgagga aaaaggactc tttgcagaaa ttccctccca gcctcccgcc
      301 gggaaaattg agtattacat agaaataacc gactcaaagg gtacaacagc acttttcagg
      361 gaaaatccgg tggttatcag attcaaggga gccgtacccg gatccattct tgtaccccac
      421 atccttttca tgttcctggc catgttcttt tcaaccctca ccgggctgat ggctgcggta
      481 aaccatccta agttcaggaa atacggtgta ataaccctcg taatacttgg tattggagga
      541 atgatcttcg gtccgctggt tcagaaattc gcttttggcg acctttggac aggtatcccc
      601 ttcggctggg acctgaccga taacaagaca ctgattgcct tcattttctg gatagcagca
      661 gtgtggctca atcggagaaa agaacgcccg gtatacagca tcattgcctc agttgtcctg
      721 atcctgatat tttccatacc ccacagcatg ttcggttccg aactggacta cgagagcggc
      781 aaagtcattc agggatcaat tcttgctttt ttcctttccc ttcggaaaaa aaattaaaat
      841 caagtgcagc attcatcgtt tacctgcgtc ttttagatga aaatctgaaa tatgttaaac
      901 gtgagtgcgg catctcagtt aaaaacaagt ccttacataa agatggctaa tgcagaagca
      961 gcattcagga acattcacca ggatctggtg gacggatgca agacaggcga tcagaaggca
     1021 cagtttcaga tttacaaatt gtactacaag gcaatgtaca atacgagtct gaggatcatt
     1081 aacgacacaa tggaagccga agacataatg caggaatcgt tcctgtcggc ctttgaaaaa
     1141 attgatacct actccggcac cgtgagtttc ggagcatggc ttaaaaaaat agtcatcaac
     1201 cgttccctcg atgccctgag cagaagaaaa gcgatctttg aagatattga ggcccacaca
     1261 ggtatcaggg atgaaggatc tgacgatgta tcgaggcagg aagaaatcga tataaaggtg
     1321 gatgagataa aagaggcaat aaacaatctg cccgacgggt acaggataat tctctcgctt
     1381 taccttctgg aaggctatga tcacgatgaa attgcagaga tactcggaat ttccagcagt
     1441 acgtccagat cacagctttc aagggccaaa cagaaattga tttcggaact taagaattat
     1501 tgaaaatcag cggcaatgaa aaatatagaa gacataataa gaaacaacag ggatcttttc
     1561 gacactgcgg aaccacagga tggtcacttt gaaagattct gctccaggct ggaggaaaga
     1621 tttcctgcgc acaccatggt caggcgaagc attgtacctt atctgctgaa ggcagcagtg
     1681 gtcacattgc tggtaaccct ttcatcattg tggacatggg aacatttcct gaagcccggc
     1741 agggacagga tgaccctggg cgatgtatca ccacagtacc gggaagttga aaactattat
     1801 atgtaccagg tgaacctgat gagcaatgaa attgcaagtc tcgatgaaaa tgatgaggga
     1861 cagaagcaaa tgctgctggc tgaaatgaaa agcatggact cagtttacat ccagcttcag
     1921 aaagacctca gggcaaatcc caatgatgaa agaataatca atgcaatgat agaacactat
     1981 cagacgaaac tggaagtaat gaactatata gtcaaccagc tcaagataat cagaaatgat
     2041 aaccaaacaa ggaaggctca tgaaaactca ggcttataaa tcaggaatta tccttgtatt
     2101 atttatcctg ctcacttcgg gagcaatccg gggccagcag gcggtaaagg attttcacaa
     2161 ggaatatacg gcaggacccg gtaccaccct tgacgtcagc aacaggtatg gcaatgtgac
     2221 tgtcgaaaca actgacggca acatggtaat cattgatgta agggttactg ttgatcttcc
     2281 cagcagggaa cgggctgaaa agctcctgtc gtacattgat gtcaactttg ccgaaagcga
     2341 taatgtgatt actgcaaaaa ccgttatcga cgataaattc agcttctcgg gatggggagg
     2401 gagtaacagg aaattcagca taaactataa gataaaaatg cctgcccgga taaacttcac
     2461 cctggcaaac aaatacggca acacagacct tgatgaaatt cacggtcttg tgaacctgga
     2521 tatcaaatac ggcaacctca ctgccgaaaa tctttccagg ggaaatgaaa aaccactgaa
     2581 tgcacttacc attgcctatg gcaatgcttc aatatcatcg gccagctggc tcgacattaa
     2641 ctcaagatac gtgggtaacc tcaggatcga caggatccag gctcttctta tcagcagcaa
     2701 gtattcaaaa atcaggctcg gagaaacaag ctccgttgtg ggagattcaa aatacgacaa
     2761 cttccggata agtaaaatca ataacctcgt tctcgatgcc ggctattcgg atatagttgt
     2821 ggaaacattg ctgaagaaac tgaaatttga aggaggctac ggagccctga ccgtcggaga
     2881 aatacctgcg gggttcgaat taatcgatac cgatacaaga tacatagggg tcaagctcgg
     2941 gatcgatgat aatgcaagct acaagctcga tgccaggctt tcgtacggga atctcaaatt
     3001 caatgaagaa aattaccgca accagaagag gatagctcag agcacttcaa atgaaataca
     3061 gggaattgtg ggaaatgaaa ctgatcccac atcagttgta aaagttcatg catcctacgg
     3121 gtcggtaagg ttgacccggt aatgacttca cggtaataac cggatcatgc ccgacataat
     3181 ataattggtg cacagttaca cgactgtgca ttcatattgt gctgtaaagg caaaataacg
     3241 gaagctaaaa atccatctcg acaacagtaa tacccgaacc tcccatttcc acatgttcat
     3301 ccctgaagga tcttacaaca ttcactgttg ccaggtaatc ccttacaagc tgcctcagaa
     3361 tgccgttacc cttgccatga agtatcctca ggttcctgtg ctggaccaga agggcatcat
     3421 caataaaatc gcgtacaata ttcaatgctt cctctcccct taccccgcga atgtctatct
     3481 cgggcctgaa actgcttctg cgctgttcga tgacgggatc tgacctgtgc acagcctggc
     3541 gcgacctttc gcttttttta agctccgatc tgctgatcct ttcaatcttt tcgagcggaa
     3601 caaggaacct gatactgcct gcctcaactg ttgccattcc tgattcaatc ttcacaattt
     3661 cccccgccgc ctgtgtttca accattctga cagcatcacc ggccgacagg ggttttacct
     3721 tttcagccat tggctctgcc ttcttcctga cagcttccgg ctttatcttt tttgccttct
     3781 gtgccagacg tgttgctttc tcttctgcgt cggtaatcac cagttttggc tcttcctcta
     3841 tattctccct gaattcatcg agcttctgcc tcagatctct ggtcttttcc ctttctgcct
     3901 gtgcttcctt aatctgacgt atggtattct caatgatacg gtttgagccg tcaagcaagg
     3961 cccgggcatc gtcccttgcc ctggatatta tctccttccg gagatttttt gcaccggcca
     4021 gttccttttc atattcggca atcagttctt caagtcgctt ttcctgatgc ctgatattct
     4081 gccttttggc ttcccagtat cttttatccc ttgcaatatc cttcaggtgc ctgtcgtaat
     4141 ttatgttctt cacccccgct ttctccgaag caattgcgag aacatgctca ggaagtccaa
     4201 ttttctttgc gatctcaaag gcaaatgaac ttccgggcct gccgatgctg agctgaaaca
     4261 gcggttgcat caggtgattg tcgaaaagca ttgcgccgtt gacaatgcct tcggtcaggg
     4321 atgcaaaatg cttgagattt gtgtaatgcg ttgtcagcac tccatatact ccggccctgt
     4381 tcaattcgcc gagtacagcc tcggcaatcg atccgccaag catcggctcg gtacccgttc
     4441 cgaactcgtc aatcaggatc agggtttcag aagaagcgtt cctgacgaaa aacttcatgt
     4501 taagaagatg tgaactgtag gtactcaggt cattgtcaat actctgttca tcaccaatat
     4561 caatgaagat gtttttgaag atgcccgatt ccgaaccttc cccgacgggt atggtgagcc
     4621 cgcactgcag catgtattgc aacaaaccca cagtcttgag acaaaccgat tttccgccgg
     4681 cattgggccc cgaaataagc aggatcctgt cgttccggtc tagccttata tcaagtggaa
     4741 ccacctttct gcccggggtt ttccggaatg ccagttcaag cagcggatgg accgcccttt
     4801 tccagctgat acccggaacg tccgcaagag caggcttgat ggaaccaagt tgattgccaa
     4861 acaatgcttt ggcccttatg aaatccatct gccccagaaa atcgttggaa agcaggagat
     4921 catcaatgta cgggcgtata tcatcggcaa aagatgttag aattctcact atctcacgca
     4981 gctcttcgta ctccagttcc acgatctcat tatttatctc cacaacctcc acgggttcaa
     5041 cataaacggt ctttcctgat gccgactgat ccaatatcag gcccctgatt ttccgcttct
     5101 cggaagcatt aaccgggatc actccccttc cgtcgcgaac cgtggccacc gtgtctgcct
     5161 cgacgattcc ttcagccttt gcctgcctta aaattgcatt caggcgcttt gaagcctgcg
     5221 cggtgagcga agaaatactg ccgcgtatct ccttcagccg gggcgaagca ctgtctttta
     5281 tttctccctt cctgtcaatg atccggtcag ctgcatcaat cacataggga aaaaccttca
     5341 cattgccgca tagcgatctg agaacagggt atttatcact ttccttcctc ctgaaaaatg
     5401 ccaggattga cctgactgtc tccagggagc gcctgagttc aaacacttcc cttgtctcag
     5461 gaaatgtacc ttctatcctt atcttgttca gacagccgga aatttcaaaa taatcttccg
     5521 agggaaaaga gtcctcaagc aggagcagct gctgaaattc aaaggtttct gacaaccgtg
     5581 catcaaccgg ctgcaggtca gtcatgaatt tcatctcctc aatcaattcc gaacccattg
     5641 tactgaggca gttatccttc agaagggcgc ggatcctgtc aaaacctgtc ttagcttcaa
     5701 aattgccggg atagatcatg gtctttttgc ttcaggtttg atgaaaccgg attccgggtt
     5761 ttcttttcct gcaaatttac cttattaaat cataacatct gagctgctaa atctccataa
     5821 tatgcagaat attctgagcg acggcctccg ataatttaat atctttaaca tttgagaggc
     5881 atcaaattaa tatattttcc tacttttgaa gtttgacttt aaaataatta ctcctatgaa
     5941 atcattgaat tacctggcaa cactgatcgt cctgatattt ttcccggttc tggtcaatgc
     6001 ccagactgaa accgttgacc tgaacatgat ctacaggata aaacaggaag ggatgaagaa
     6061 ctcggcaatt gaggaactgg ctttcggatt gacagatctg tcggggtcaa ggctgacggg
     6121 gtcgcctggc cttgcacggg ggaacgaatg ggctatgaaa aaaatgttgg agctcggttt
     6181 ccagaatgta aggattgagg aagctgctga tttttcccag ggaggatggg acaatctgaa
     6241 aacctatgca gccatgatct caccctatta tataaatttt gcctgcaacc cggttgcatg
     6301 gacaggaagc acgaacgggc ccgtaaaggc agaggttatt ctcctcgacg tcaaaacgga
     6361 ggaagatctt gataaataca aggggaaatt atcaggaaag atagtaatga tgccatcgac
     6421 ggcttcctat gaagtgagtt ttgagcctct tgcaaccagg tacactgacg atgagctgaa
     6481 agaactctca atggctgcag tcaggcctca gggaggaaga gtgcccgccg actgggccaa
     6541 ctatatggct cagagggcgc tgcagatgaa aattaccggg ttcctgaaat ccgagggagt
     6601 agcagttatt cttaacaatt ccggattttt caatgttccc aggtcagctt ctgccagtta
     6661 taaggccgga gatccggaac cggttccgca acttagtatc ccggtggaag catacggacg
     6721 gatggaaagg ctgattgctc attccgtcag ggttgaaatg gaaatagaga tcataaataa
     6781 gttcctcgga ccacagaaag tctacaacgt gatcggtgaa attcccggaa ccgacaagct
     6841 tttaaagaat gaggtggttc ttctgggagc ccatatcgat tcatggcacg gaggaaccgg
     6901 agctgccgac aatgcatcgg gttgcgtggt aatgatggaa gccctgcgca tacttaaaaa
     6961 tctcgacgtt gcaccccgga ggacaatcag ggttgccctg tggggaggcg aagagcaggg
     7021 cctgattggt tcaagggcct atgcggacaa atatcttgcc gaccgtgaaa caaaagcaca
     7081 taagcctgat tttgacaggt ttgcagcata cttcaacatg gataacggca cagggaaata
     7141 cagggggata tacacccagg aaaatgatat ggtgcggccg atattcgagg aatggcttaa
     7201 gccgttcgcc gacatgggag ccgcaacagt ttccggaagg aataccggcg gcaccgatca
     7261 cctgtcattc gatgcttacg gactccccgc cttccagttt atccaggaca aaattgagta
     7321 cggcaggggt tatcactctg ttatggacac ctatgaaaga ctcctcatga acgatctcag
     7381 gcacaatgcc ataataaccg cagcgtttgc ctacaatgcc gccatgagaa actcaaaact
     7441 tccgtcaaag cctgccatga agcctgccac gaatgtaaaa cagccacaaa tggcaccgtg
     7501 gatgaattag actgtatccg gcaacattga ttatcacagg tcaggcatat tcataaggga
     7561 agcgaaggag cccattgcac ctatgcaaaa acgggtatgg cggaaaacca taaaatccgg
     7621 ggttcacgca tgcatcctgt caggccgcga cccagcgacc ggacggttcc ggtatggtct
     7681 gccggtgtcg aaagggcata tcctgaattt aaatgcagtt taaaaagatg aaggctcctg
     7741 tcccttgttt cccttatcca gtcctttcat ggacagctgt atgcgtttac gctcagggtc
     7801 aatatcaaga accttcacca taacatgctg atgcagcttc actacatctg cagggtccct
     7861 gacgtaggtg ctgctcagat ttgatatatg caccagtccg tcctgcttga tgccgatgtc
     7921 cacgaaggcc ccgaattttg ttatgttggt cacaattccc ggaacaatca ttccgggggt
     7981 gagatcctcc atcgtatgga tatcggcaaa actgaattcc tttatttttg aacgagggtc
     8041 ccttccgggc tttgcaagct cttccgtgat atccctgagc gtgggcaggc ccgttgtttc
     8101 cgttacatag ttctcgagcc tgatttgttt ccttttttcc tgatcggcca taagttccct
     8161 gacgtcgcat ccaaggtcag ccgacatttt ctcaacaaca tgataacttt caggatgtac
     8221 ggcacttgaa tcgagggggt tcccggcgtt ccttatacga aggaatccgg cacattgttc
     8281 aaaagccttg gcacccatcc tcctgacatt caggaattca tttctcgatc ggaagggtcc
     8341 gttctcggta cggtaatcca ctatgttgcg ggcaagctgg gggcccagcc cggagacgta
     8401 ggtaagcaaa tgactgcttg ccgtgttcac ttccacaccg acggcgttga cacagctgac
     8461 caccacctca tcgagacttt gctgcaattt ctgctgatcc acgtcatgct gatattgccc
     8521 cactcctatc gatttcggat cgatcttaac aagctccgca agcggatcca tcagccttct
     8581 tccgatggaa acggcgcccc tgaccgttac atcatagtcg gggaactcct cccttgcaac
     8641 cttcgatgcc gaataaactg aggcaccggc ctcattgaca acaaagacct ggatatcttt
     8701 ttcaaatttt atccatttga tgaactgttc ggtctcccgg cttgcagtac cgttacctat
     8761 ggctatcgct tcggttttgt aggcgttcac cagcgttaaa agtttcctga ccgacagcgc
     8821 cgtctgattc tgcggtggat gaggaaatat tgtctcatta tggacaaggt ttccctggcg
     8881 gtcgaggcag acaatcttgc agcctgtccg gaagcccgga tcaatggcaa gtacgttttt
     8941 ttcacccagg ggtgaagcca acagaagctg acgcaggttc tctgcaaaca cggcaatggc
     9001 ttcaccatcc gccttctcct tcgacacatt cctgaattcg gtctcaagcg aacctgaaag
     9061 cagccttttc cagctgtcgg ccaaagcttc cccgacaagt ctcgatgact cgttccggcc
     9121 ctttatgaat aaagacttta atatgtcaat cgccttttca tcctcaggca ataccgaaag
     9181 gcgaagaaac ccttcctctt caccccttct catggcaaga agcctgtgtg acgggcattt
     9241 cctcagcggt tccgaccaat cgaaataatc actgtattta ataccctcct gttcctttcc
     9301 ccttacaact tttgaactga tgacagcttc ccgttcaaaa agaatcctca gccgggcccg
     9361 ggctttctgg tcttcactga tccattccgc tataatatca catgcaccgg ccagagcctc
     9421 ccctttgtcc gccacagaat cgcccaggaa ctcctctgcc ttttttcccg ggtcagtctc
     9481 accctgttcc attatcagtg aagcaagggg ctcaaggccc ttctcctttg caattgtcgc
     9541 ccgtgttctg cgttttggcc tgaagggcag gtataaatcc tctatctccg acattctggt
     9601 ggcagcctcc agcttctgcc tgagttcagg cgtcatttta tcctgctcct ctatcgacct
     9661 gattatgctc tcgcggcgga tgtcaagttc cttcagcctg ttgtattcat ccctgatttg
     9721 aagaagctta acctcatcga ggcttcctgt catttccttc ctgtacctgc tgatgaaagg
     9781 aatggtcgaa ccgtcttcca gcagtcttat tgtattctca acctgccata aatgcaatcc
     9841 aagcttttcc gcaaccagtg aaatgttctt ttgagtaccc atattaaaat aattccaggt
     9901 gttaaccata atttccggaa atcgtattat ccggcaatcg ctgcaaaagg ccgggctgga
     9961 ttttcatact ttccgcctga agcacctgcg cgcaacccac gggagatcct gaagaaaacg
    10021 atcctcaacc ctttttcgta agtctgtcat atatctggtg acagatccat gcatcggttg
    10081 cagcataaat tatctgtgct tcggttagtt cgtcagcctc ccagtcggat acctgctgcc
    10141 gtttggatat cctgatccca agtacaatcg ctgccagctt cttcagcccc gaattctgta
    10201 tcccgtattc cttcacgaat ttctgaaggt caataaaccc cgaaggtacg aagccgttga
    10261 tacgtttcag gaagctgata tcgtcatgaa ctgccacccc ggccttgatg acgtcgggac
    10321 tggccagaag ttcagtcaat ggatcgggaa taccgatcct gttgatcctg aacagacagg
    10381 caagctcgct gtttgccagc tgaagcagcg aaaccctgtt tttcctgcct ttcctgaaag
    10441 agggtttggt ctcggtgtca aatcccagaa agggagaacc aatcagcctg ggaaaagttt
    10501 tcctgaaagc cgccatatta tcaatcagca caatctcacc tctgaaccag gacaattcct
    10561 tcaatgctat ttcttctgcc gaaatagtgg caggataatt attcctcagc atcttccctg
    10621 aattcctttt gacggcggga aataaaccat tcggatttat ccctttcata ggaatcactc
    10681 ccgccatttt ctgcaatatc tgtcgattca cccgaataga tgaacctgtg gatagcccta
    10741 agtgaattta ccagtttttg accccagata ttctcaaaat tcatccggca ttcccagaca
    10801 gcgtcattca tgacctcctt tgagccggtc tggtacaata acaggaagtc cttgagatcc
    10861 tggtaaatat cagcaaaatt ctcagacagg gaggcaataa ccggctcccc tccgtccgca
    10921 atcctctcgt caaacacctc gtaatagtca tcagcagcgc caaaccggtc cttcaatgta
    10981 tcatgaaccc tgttccagtc agcctcggta acgaactttt cattgccatc ctcaaaaacc
    11041 ggttccgggg ccggaagaag ggaagccttg atgtacaggt atggcagaag acgttgaaat
    11101 attctcagca gttcatcacc aggaacttca gaaccatgct cggcgtattt gcaaaactca
    11161 ttggctgcag caacaaattc cacaacgctt cgcgaataga ccggatctga tttcttattc
    11221 atttcattaa tatacctccc ggcaaaataa gtcataaatt caatgcgatg tttcgcatca
    11281 accgaacaaa gtgcagaatt tgcttgccgt tgagaaataa attgaataaa ttccctaatt
    11341 gataaaaacc tggctccgcc atattgttaa gcataaataa gccggttgat atcagtaatt
    11401 taattggacc agacctgttt tgtcatgaaa aagttcctga tcgtatttac tgctttgctt
    11461 cttatacatt taaccagcag tcatttcctg aacggacagc cggcaggatt aacccgcgga
    11521 tggagggccg gagttgcaaa agtcaatatt acaccggagg gatccatttg gttggcaggt
    11581 tatgcttcac gcgatcatcc gtcggaagga gtgttgcacg atatctggtc caaagtcctg
    11641 gttatagaag attccctggg aaacagggct gcattgataa ccaacgatct ccttgagatc
    11701 ccgattcatt actcaacaca gatcagggac aggcttgaaa aagaatatgg cctgcaccgg
    11761 tctcaggtaa tactcaattg ctcacacacg cattcggggc cggttatgag ggacaccctg
    11821 gccaactggt atccactcga cgatgcaatg gaaaaaaagg taaagaaata ctctgaagat
    11881 tttgtcgaaa aaatagtgga aatggtcggc agggccatct acaggatgat tcctgttgaa
    11941 atcagtgccg ggaacggaac agcaaggttc caggtaaacc ggcggaataa ttccgaggca
    12001 atgagtgaat attacaccga tctgaagggg cccaatgatt acgctgtgcc cgtgctcagg
    12061 atcgacagga aacagggagg gatcatggca attgttttcg gctatgcctg ccattcaacc
    12121 gttctgaatt tttatcagat aagcggtgat tatcccggtt ttgcccagat tgaactggaa
    12181 aacctttatc ccggtacaac ggcactcttt ttccagggtg ccggagccga ccagaatccc
    12241 cttccccgca gaagtgtccc gcttgcccgc cagtacgggc aaaccctttc ggcagcagtc
    12301 gacagggtcc tcaatgaaaa aatgaaaaga cttgtcccgc tgctgaaaac cgcctattct
    12361 gagattccgc ttcagtttga cggttcccct cccgacaggc aggaattgct tggtcatatc
    12421 cacgacactt catcaactgc gccatatctg aaactgatgg cacaaaccct tatccgcagg
    12481 ctcgacaacg gagaggtatt caggaacggg tatccttatc cgtgccaggt ctggaagctt
    12541 ggagaccaga tcatcatggc ccttggagga gagcttgtgg ttgaatatgc cctggagctg
    12601 aaaaaaaggt tcggccagga tgtctttgta atgggatata ccaatgatgt tatggcctac
    12661 ataccttccg acaccatcct cagcgaaggg ggatatgagg gtacacgctc ggtttttttt
    12721 accagggcat ggagccgtaa cacccaaaca gctattataa atgaaatgtc gagcctagcc
    12781 gggaaactgg gaatcacacc tattgaggca cctgaccaca atgaaacggc aggaagataa
    12841 tcctgccctg catcctgaat acctgaatta aaacaaatca acccttaatc tgaaaaagaa
    12901 atgacgaaac gcattttaac aagaagacgt tttattgcct cggtgtcagc aggttctgtt
    12961 gctgccattg ccggctccgt accggtgctt ggaaagactg gcaatgacgc cggcaggctg
    13021 gccattcttg gaggcatccc tgcaatacct cccaaaaaat ggcccgactg gccgtacgtc
    13081 gatcagaaga tgatcgacgc catgatcaga accacgaaaa gcggaatatg gtcaagaata
    13141 cagtcagaaa ccggatccgt tccgaccttt gaaaaggaat ttgctgctct tatgggcgca
    13201 aaaagatgcg taacaaccgg atcgggtaca caggctctga gctgctgtgt agaggcaatg
    13261 ggaataggtg ccggcgacga ggtgatcaca tcgccatata ccgacatggg gactatttcc
    13321 tccattctta catcgcgtgc cctgccggtt atggcagatc ttgatccggc atcattccag
    13381 attgatccgg ctgatgttga aaggaagatc acacctgcaa caaaagccat catgcctgtt
    13441 gacatgggag gacagccctg caacatggcc cggataaatg ccattgcaaa aaagcataaa
    13501 ctcagggtga tcgaggattc gtgccaggga cacctcggtg aataccaggg aaaaaaatta
    13561 ggaaccgtgg gtgagctggg atgtttcagt tttcagacat ccaagaccat cgcctgcggc
    13621 gaaggcggtg ctgtgatcgg caatgatgaa gcgctgatgg acagggtgtt ttcggttatg
    13681 atgaacggca gccacaggat catcggaacc aagtacagga tgaccgaatt tgaagctgcc
    13741 ctgcttctca gccagttgga aggagcaaag gaaagattcg cgatccgtaa ccggaacgct
    13801 gcctatctga cctcaaagct caggaaattc ccggggctgg tgccgcagaa actgtacccc
    13861 ggaacaacca gcggctccta ttacctttat atgatggcat ataaaaagga gtatttcaac
    13921 aatgctgaca ggagcaaatt cctgaaagca ctggcagccg aaggaataga actgagcccg
    13981 tatattgcaa agggcctgca cagggaaccc tggacggagt t
//
LOCUS       DAOOYG010000067        13998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4416_length_13998_cov_7.736355, whole genome shotgun sequence.
ACCESSION   DAOOYG010000067 DAOOYG010000000
VERSION     DAOOYG010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13998
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4416_length_13998_cov_7.736355"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PK288_07115"
     CDS             complement(<1..1157)
                     /locus_tag="PK288_07115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPM18730.1"
                     /translation="MNRNDLLNSLLENNDRQWDIIVIGGGATGLGIALDAVTRGYRTI
                     LFEQSDFSAGTSSRSTKLVHGGVRYLAQGDILLVKEALHERGLMLNNAPHLTSDQEFI
                     IPAYTWFDCMLYTVGLKFYDFLAGRLSMGRSYFISGPEVKSRLPLINPKGLKGGIVYH
                     DGQFDDSRMAIALADAFVNRGGIALNYFRVTGLLKDGNNRISGVMTSDMISGQEHLVR
                     GRLVINATGVFADEIHRMDDPMSKPTIRPSQGIHLVLDGSFLQSRSALMIPKTDDGRV
                     LFAIPWFGKVVTGTTDTPLDRISREPRALDSEIDFILGTAGRYLVRMPGRDDILCIFA
                     GLRPLAADPANPGSTREISRRHKIIISRSGLLSIVGGKWTSYRRMAEETID"
     gene            complement(1226..2341)
                     /locus_tag="PK288_07120"
     CDS             complement(1226..2341)
                     /locus_tag="PK288_07120"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HPM18731.1"
                     /translation="MERKIYSLLTLIMMLIGFGTADGQVSRLYAGKYAGQGEKGLLVL
                     DVDFARGTIAAISENDAGINPSYFCISRKNSMLYAINEVGRFNGQRSGSVTTLKLDIK
                     KGTAEKITDLAVPTGGPCYVSLSPGEDYLLIANYGSGSVAVVRLDAGGVPSEVSDTII
                     FRGPEGTRSHPHMIAPGPEGKKIYLTDLGLDRIAIYDLDKASGKLKNTGYAQLAKGAG
                     PRHFAFGKDGSKMYVVNELNSTLTVFDVAKNGNLTEIQTLSALPPEFTGKSYCGDIHV
                     SNDGKFVYGSNRGDNSITTFKISADGRVSVAGFTPCGGEWPRNFVIDPSGKYILVGNQ
                     NSNNISVHKIDPVSGIPGPQISNFDTKSPVCLKFITP"
     gene            complement(2382..3320)
                     /locus_tag="PK288_07125"
     CDS             complement(2382..3320)
                     /locus_tag="PK288_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPM18732.1"
                     /translation="MDNSRRDFIKKATATGAAIMAAPVLSGSQKKQAAKKAPEAVAPF
                     KLKYAPSIGMFREHAGTDIIDNIKFCHDMGFRAMFDNGLMSRPVADQELLTGELRRLG
                     MDFGPFVLYADFGVTSFVLNKPEIRQMLIGKMKEGVEVSKRTGARFALVVPGRYDERL
                     HRDFQTANVIDNLRYCCDIVEPAGLTIVLEPLNVLHDHPGLFLTGIPQAFAICRAVNR
                     KSCKIINDIYHQQITEGNIIPNIEAAWDEIGAFHCGDNPGRNEPGTGEINYKNVFKYI
                     HSRNYDGVICMEHGIREKTREGEARVIQAYRDCDNF"
     gene            complement(3351..4688)
                     /locus_tag="PK288_07130"
     CDS             complement(3351..4688)
                     /locus_tag="PK288_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18733.1"
                     /translation="MKKSSISRRDFFGKAAAAGIVLPTIITGCSSQPRKVVAVPNFLD
                     QAPDGAVLKAGVIGCGGRGTGAAINFLSAGPNLQVTALADTFKDRVDNCRERILQEKG
                     QEVPAENCFVGFDAYQKVIDSGVDIVILATPPFFRPEHLAAAVQARKHIFSEKPVCVD
                     PVGARSVMATAKKAEGLELSIVTGTQRRHQRDYVATWQQVQQGIIGELTGGNVWWNGG
                     KLWHRDKNPDWTEMEWMIRDWVNWTWLSGDHIVEQHVHNLDVANWFTGKHPVKAVGMG
                     SRLRRVTGDQYDNFSVDYTFENGTHIHSMCRQINGCANNVSERLQGTKGSTNCQNSIL
                     SLDGEEIWKYEYPLDKDQKPTNTVSVDPYVQEHIDLVTAIRTGKPFNELENTAISTMV
                     AIMGRVSAYTGKETTYEEMMNSDLKLGPKVFVFGPVDIPKEVPIAGLPYVPQA"
     gene            complement(4796..6334)
                     /locus_tag="PK288_07135"
     CDS             complement(4796..6334)
                     /locus_tag="PK288_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM18734.1"
                     /translation="MKKLMLWFTPGLIAGALVILGAQRAVDITSTNKFCVSCHIHPVA
                     DAEWKKSVHYDTKSGYRVGCAECHLPPKGEGFLWEKGKTGLRDLWSYIFRDSASFDWE
                     AKGRLEYARKHVYESSCIKCHENLFPAKLTKEGEDAHLYYTQQPKTPDLYCINCHLNA
                     GHYIEGYTHGSNVTFGKVSSAPKEIFSEPAIVTGFENFTEKIPASSVSFEMVAIPGGT
                     FEMGSPEDEPFRKPDEGPVRTVEISPFFMGQTEVTWDEYLAFYVQTSVEGRSTDTEGL
                     RNEISAGARTDAISGATPPYGQPDQGWGLGQRPAISFSFHAAETYCKWLSQVTGKNYR
                     LPTEAEWEYACRAGTTGPYFFEGDPRKFEKSGLRARISKNDTAVINSYVVYNENSPSK
                     TQPPDFVKPNPFGLINMLGNVAEFCSDWYQPNAYSEYPGGTIRDPKGPETGSEHVIRG
                     GSFMNPAGLVRSAARDYTRTEAWLKTDPQMPKSIWWYSDCFNVGFRVVCEYDEKTGKE
                     VKKP"
     gene            6707..8137
                     /locus_tag="PK288_07140"
     CDS             6707..8137
                     /locus_tag="PK288_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HPM18735.1"
                     /translation="MKKYFFIAIFLILNFTLLAHPWKPSHYVIIDTDGGIDDMRAITM
                     FLASPDVRVLAVIASSGTLSAENTYRKVRSLLNSYFHEGVPVGINSSVSFKSPDISMA
                     LKTQWGDETNLSPQDAPAALDLISWVFSEEKTQISFVSLGGLTTAWTAFNAIPSFSRQ
                     VKEIVWSADGFSDTKGFNYNIDKKAAAGILKGSIPVKIIKPAGEKEFYNTELIADISG
                     ICSIYAEKLSVFFSSATDAGHDFIYAATDETIPLFIHFPALFNTTASGINSESRAADI
                     NQLREKTLAILKGETVERNQVIKEFPDNPAFYFDDLKDHVSEIIKKYGIDEWSSGVLA
                     NELHRHLGVFAIIGVKMGIRAREYFNTGVDEFRTTSYAGSITPVSCFNDGLQVSTGST
                     PGHGLLKVVDDVPAKPMAEFSYMNRKIRLTLRPDIAEKIISELKEINFIYGLDSNIYW
                     ELVRKNTIRYWLSLDRHEIFEIEEII"
     gene            complement(8140..9753)
                     /locus_tag="PK288_07145"
     CDS             complement(8140..9753)
                     /locus_tag="PK288_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HPM18736.1"
                     /translation="MADFIYEKPFQTGGDSTRYRKLAGDFVKIVESGGRKVLKVDPEG
                     LELLAAMAVSDLSFYLRTSHIEKLRKIIDDPEATDNDRFVAYVMIRNSVISARGELPW
                     CQDTGTAIVMGKKGEDVYTGANDALHLSKGIYRTFQDRNLRYSQIVPYTMFEEKNSGT
                     NLPAQIDIYATEGNVYEFLFITKGGGSANKTYLYQESKSLLNEESLEKFVREKIKDLG
                     TSACPPYHLALVIGGTSAEATLKAVKEASAGYLDDLPVTGNEAGRAFRDIAWEQRIEK
                     ICHEYGVGAQFGGKYFVHDVRVIRLPRHAASCPVGLGVSCSADRNIRARIDANGIFME
                     ELEKDPARFLPEKAPELEKPVELDLDRPMKEILAELTRYPVRTRLNLRGTLIVARDIA
                     HARIKKMIDEGQPMPKYFKNHPVYYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLFQSM
                     GGSLIMIAKGNRNRQVIDSCRKYGGFYLGSIGGPAAILAEENIKSVELVDFGDLGMEA
                     VRKIRVENMAAFIITDDKGNDFFDEYNKE"
     gene            complement(10070..10918)
                     /locus_tag="PK288_07150"
     CDS             complement(10070..10918)
                     /locus_tag="PK288_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM18737.1"
                     /translation="MKLNLFNITNFKVDGGAMFGVIPRVLWSRVCVPDENNFIVLALR
                     SIVAETDGHVILVDTGWGDKQDAKFFRHLNLSGGEGLIEGLRRLGYSGNDITDVVFTH
                     LHADHCGGATRLSNDGRTTKMVFPNAALHVSRVQWEWAVKNNPREEDSFLEENILPLM
                     HGGRLNLIDEDCELFRGFSVRICYGHTPGLMYAMIDYNGKTIVFAGDLIPTAAHVPLL
                     WNMSYDIETLRTIEEKNRLLNEALDKNHIIVFQHDELTECCTLEMTPKGIRPGRKFSF
                     SEILRK"
     gene            11160..11975
                     /locus_tag="PK288_07155"
     CDS             11160..11975
                     /locus_tag="PK288_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPM18738.1"
                     /translation="MPAQTGKVMDNMSMPSKILKMDRKFAVYLPPDYETSQRAYPVLY
                     LLHGYGDDQTGWVQFGEVQHITDKAINEGKATPMVIIMPDANTGKVGYVNDIKGEWRY
                     EDFFFDELMPYVEKIFRIKGEKNYRAIAGLSMGGEGTFIYALHRPELFSSACPLSAAT
                     GPENIEETGQYILWPAVKRISGAERDKYFNNYSVLSLINNIQEKNKKAVRWYIDCGDD
                     DFLFEGNSLVHIAMRKKEIPHEFRIRDGGHTWSYWRSALPSVLEFISMSFHQH"
     gene            complement(11997..13175)
                     /locus_tag="PK288_07160"
     CDS             complement(11997..13175)
                     /locus_tag="PK288_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPM18739.1"
                     /translation="MDTRLKRDYIGGHSLELLRSGEQFFAENVRLINEAKRYIHFQTY
                     IVDEDQTGLRVIDALIRAAERGVRIYLLLDAFGTKYLSGGLIKRIESSGILFRFFSPV
                     FIKKGFQLSLRLHHKVLLVDGEAAIIGGMNYADRYHGTPQKKEWLDFAVLMKGPECVH
                     VNSILRRHWNKTFISRQERAHETASQIKTYEENIRLRVLENNWYRNKIEILRSYRHAF
                     KHAHLRMIILASYFLPGRLERKLLRQASTRGVDIKIVLTAESDAPMFKRATRFLYEYI
                     LRNNITIYEYLPANLHAKVAAVDGKWCTIGSYNLNHISDYGSIEMNVGVTDTAFTRKF
                     EDLLSEIIRNDCRQITFEEYLRRRTWYSQLRGWMSYQMIRLFMRIMAQMTSRKKKPSN
                     "
     gene            13414..13884
                     /locus_tag="PK288_07165"
     CDS             13414..13884
                     /locus_tag="PK288_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18740.1"
                     /translation="MKRGFLIVLLSLILASSSYSQEYKTSMGIRAGLPIGLTLKHFLN
                     RDYALEGMLATRWGGITLAGFLEKEHRVKNHHGLYWFWGGGIHSGIWDAGYNPRLKAG
                     YTGPVFGIDGIIGLEYTLDYHPLNFSFDLYPSLNLIGSAGWGGINGAVSVRYVF"
BASE COUNT         3600 a         3426 c         3116 g         3856 t
ORIGIN      
        1 ctgtcgattg tttcttctgc catccgccgg taggaggtcc atttccctcc tacaattgac
       61 agaagtcccg acctggaaat gattatctta tgacggcgag atatttccct ggttgatccc
      121 gggttggcag gatctgcggc aagcggccgc aatccggcaa aaatgcacag gatatcgtct
      181 ctacccggca ttctgacaag atacctgccg gcagttccta aaataaaatc tatttctgag
      241 tcgagggcac gcggctcgcg ggatattcta tcaagagggg tatcagtggt gcctgtcacc
      301 accttaccaa accatggaat ggcaaacagt acacggccgt cgtccgtttt cgggatcatt
      361 aatgctgaac ggctctgcag gaaactgccg tcaagcacca ggtgtatgcc ctggctcggg
      421 cgaattgtag gttttgacat cggatcatcc atcctgtgaa tctcatctgc aaaaaccccc
      481 gtggcattta taaccaatct gccgcggact aaatgctcct gtccggaaat catgtcagac
      541 gtcattaccc ctgatatcct gttgttcccg tcctttaaca ggcccgttac cctgaaataa
      601 ttaagggcaa tccctcccct gttgacgaag gcatcagcaa gagcaattgc catcctggaa
      661 tcgtcaaact gtccgtcatg gtaaacgatt ccccccttga gccctttcgg attgatgaga
      721 ggaagccggc ttttgacctc agggcctgag atgaaatatg atctgcccat gcttaacctg
      781 ccggcaagga aatcatagaa tttcaatccc acggtataga gcatgcaatc gaaccatgta
      841 taagcaggta tgataaattc ctggtctgat gtcaggtgag gtgcattgtt cagcattagt
      901 cctctctcat gcaatgcctc cttcacaagt agtatgtcgc cctgtgcaag ataacggacc
      961 cctccgtgta caagttttgt gcttctggaa gaggtacctg cggaaaagtc tgactgctca
     1021 aacaaaattg ttctgtaacc ccgggtgaca gcatcaaggg caatccccag accggtggcc
     1081 ccgccaccta ttacaattat atcccactgc cggtcattgt tctccagcag gctgttcaac
     1141 agatcattac gattcataat gaataattcc ggtcagagat atgcaatttt caatttcccg
     1201 gattggatca atccgtttca ggacttcaag gtgtaataaa cttaaggcaa accgggctct
     1261 ttgtatcaaa atttgaaatt tgcggtcccg gtattcctga aaccggatca attttatgaa
     1321 ccgaaatatt attggaattc tggtttccca caagaatgta cttccctgac ggatcaatta
     1381 caaagttcct tggccattcc cctccgcagg gtgtaaagcc tgctacggaa accctgccat
     1441 cggcagatat tttgaacgtt gtgattgagt tatctccacg gtttgaacca tagacaaatt
     1501 tgccgtcatt agaaacgtgt atgtcgccgc agtagctttt accggtgaac tccgggggca
     1561 gtgctgacag ggtctggatc tcggttaaat ttccgttttt tgcaacgtca aaaaccgtca
     1621 gggtggaatt aagctcattg accacataca ttttggaccc gtcctttcca aaggcaaagt
     1681 gtcgtggtcc cgctcccttt gcaagttgag catacccggt gttcttaagc tttcctgaag
     1741 ccttgtcgag gtcatagatt gctatccggt cgagcccgag atcggtgaga taaatcttct
     1801 ttccttcagg gccgggggct atcatgtgcg ggtgagagcg tgtcccttca ggacccctga
     1861 aaataattgt gtcacttact tccgaaggaa cacctccggc atcaagtctg accactgcca
     1921 ccgagccgct tccataattt gcaatcagca ggtagtcttc cccaggtgaa agagaaacat
     1981 agcacgggcc gccggtggga actgcaagat ctgtgatctt ttcagccgtt ccttttttta
     2041 tgtcgagttt tagtgtggta acactgccgc ttctctgacc attgaatctg ccgacctcat
     2101 ttattgcata taacatactg ttctttctgg aaatgcagaa atatgaagga ttgataccgg
     2161 catcattttc cgaaatggct gcgatggtgc ccctggcaaa atcaacatcg agtaccagta
     2221 gtcctttctc tccctgtccg gcgtattttc ctgcataaag ccgcgatacc tgtccgtctg
     2281 ctgttccgaa gccgatcaac atcattatca gagtaagcaa tgaataaatc tttctttcca
     2341 ttttttattt tgtcattttt ccaatagctg gttcctgatt tttaaaaatt gtcacaatcc
     2401 ctgtaggcct ggatcacccg ggcctcacct tccctggttt tttccctgat cccgtgttcc
     2461 atgcatatca ctccgtcata attcctgctg tgaatgtatt taaacacatt tttgtaattg
     2521 atctctcccg ttccgggctc gtttctcccg gggttgtcgc cgcaatgaaa ggcgcctatt
     2581 tcatcccagg ctgcttcaat gttgggaata atgttccctt ccgttatctg ctgatggtag
     2641 atatcattta tgatcttgca tgatttcctg tttacagccc tgcagatggc aaaggcctgg
     2701 ggaattccgg tcaggaacaa tcccggatgg tcgtgcagca cgttgagtgg ttcaagtact
     2761 atggtgagtc ctgccggttc aacaatatcg cagcaatatc tcaggttatc aatcacattg
     2821 gcagtctgga aatcccggtg cagcctttcg tcataacggc cggggacgac gagtgcaaat
     2881 ctggctcccg tccgctttga tacttcgacg ccttccttca ttttaccgat cagcatttgc
     2941 ctgatctccg gcttgttcag aacgaaagat gttaccccga aatctgcgta gagaacaaag
     3001 ggaccaaaat ccattccaag ccgtctgagt tcaccagtca acaattcctg gtctgcaacc
     3061 ggcctgctca tcagtccgtt gtcaaacatg gccctgaagc ccatatcatg acagaactta
     3121 atattgtcga taatatctgt accggcatgt tccctgaaca tgccgattga gggagcatat
     3181 ttgagcttaa agggagcaac ggcctcaggg gcttttttcg cggcctgttt tttctggctg
     3241 cccgacagaa caggagctgc cattatggcg gctcctgtcg cagttgcctt cttaatgaaa
     3301 tcccttcttg aattatccat atacgatgca tttactgttc actgaaaatc ttaagcctgg
     3361 ggaacatatg gcaggccggc aatcggcact tccttaggaa tatcaaccgg accgaaaaca
     3421 aacaccttgg gaccaagctt cagatccgag ttcatcattt cttcgtaagt tgtttccttg
     3481 cccgtgtaag ccgacacgcg gcccataatg gccaccatgg tggaaattgc ggtattttcc
     3541 agttcattga atggtttgcc tgtgcgtatt gcggttacaa gatcgatatg ctcctgaaca
     3601 tatggatcaa cgcttacggt gttcgtaggt ttctgatcct tgtcgagagg gtattcatat
     3661 ttccatattt cttcaccgtc aaggctgaga attgaattct ggcagttcgt ggatcccttg
     3721 gtaccctgaa ggcgttccga gacattattg gcacagccgt ttatctggcg gcacatgctg
     3781 tgaatatggg ttccgttttc aaatgtatag tcgacgctga aattatcata ctggtcgccg
     3841 gttacacgtc tgagcctcga tcccataccg actgccttga cgggatgttt gcctgtgaac
     3901 cagtttgcca catccaggtt atgaacatgc tgttcaacaa tatgatcgcc cgacagccac
     3961 gtccagttga cccagtcacg gatcatccat tccatttccg tccagtcggg gtttttatcg
     4021 cggtgccata gcttccctcc gttccaccat acatttcctc ccgtcagttc gccgataatc
     4081 ccctgctgaa cctgctgcca ggtggcgaca taatcgcgct ggtgacggcg ctgggtaccg
     4141 gttacaatac taagttccaa gccctcggct ttctttgcag ttgccataac cgacctggct
     4201 cctaccgggt ccacgcaaac aggtttctcg gaaaaaatat gcttcctggc ctgaacggct
     4261 gcggccaggt gttccggtcg gaaaaatgga ggagtggcaa gaatgacaat atcaactcct
     4321 gaatctatta ccttctgata ggcatcgaat ccaacgaagc agttctcagc aggcacttcc
     4381 tggcctttct cctgaagaat tctctccctg caattgtcga cgcgatcctt gaaggtgtcc
     4441 gcaagggcgg ttacctgaag atttggaccg gcactaagga agttgatcgc tgcccctgtt
     4501 ccgcgtcctc cgcaaccgat cacgccggct ttaagcaccg ctccgtcggg agcctgatct
     4561 agaaaatttg gtactgcaac aacttttctg ggctggctgc tgcatcctgt aataattgtt
     4621 ggcaatacta tccctgcagc tgcagctttg ccaaaaaaat cacgtcttga gatactggat
     4681 tttttcatgt cggaaaggat ttatgaatat cttaataaaa tactggctat caaggcaact
     4741 gtaactaatc aaatgtaaat atagttattt taaattatca ttgtcaggcc tttatttaag
     4801 gttttttaac ttcctttccc gtcttttcat catactcaca cacaaccctg aatcccacat
     4861 taaaacagtc tgaataccac cagatgcttt taggcatttg cggatctgtt ttcagccacg
     4921 cttcagtcct ggtataatcg cgtgctgcac tgcggaccaa tcctgccgga ttcatgaaac
     4981 ttccaccacg gataacatgc tccgaacctg tttcaggtcc tttcggatcc cggatggttc
     5041 ctccgggata ttccgaatat gcatttggct gataccagtc ggaacagaac tcggcaacgt
     5101 tccccagcat attgatcaat ccaaacgggt ttggcttcac aaaatcagga ggctgggttt
     5161 tagacgggct gttttcgtta taaactacat agctgttgat gacagccgta tcatttttgg
     5221 atatccttgc cctgagcccc gacttctcga attttctcgg atcaccttca aaaaagtaag
     5281 gtccggtcgt acctgccctg caggcatact cccattcagc ttcagtgggc agcctgtaat
     5341 tttttccggt cacctgcgac agccatttgc aataagtctc agcagcatgg aaggagaacg
     5401 aaatagcagg gcgttgtccc aggccccatc cctggtcggg ctggccgtaa ggcggggtag
     5461 ccccggaaat ggcatcagtc ctggcccctg ccgaaatttc gtttctgagc ccttccgtat
     5521 cggttgagcg cccctcaact gaagtctgca catagaatgc caggtactca tcccacgtta
     5581 cctctgtctg ccccatgaag aacggactta tctcaaccgt cctcaccggt ccttcatcgg
     5641 gctttctgaa aggttcatcc tcaggactgc ccatttcaaa cgttcctccc ggaattgcaa
     5701 ccatttcgaa agaaacggat gaggccggaa tcttttccgt gaaattttca aacccggtga
     5761 caatagcagg ttcggaaaag atttcctttg gagctgagga taccttgccg aatgttacat
     5821 tgctcccgtg agtgtagccc tcaatgtagt gaccggcatt cagatgacag ttaatacagt
     5881 acagatcagg agtctttggc tgctgggtat aatacagatg cgcatcctcc ccctcttttg
     5941 tgagctttgc aggaaaaaga ttctcatggc atttgataca ggagctttca taaacatgct
     6001 tcctggcata ttcaagccgt ccttttgctt cccagtcaaa ggaagccgaa tccctgaaaa
     6061 tatagctcca cagatcgcgc agcccggttt ttcctttttc ccaaagaaac ccctctccct
     6121 ttgggggcag atgacactcg gcacatccta cccgatagcc ggattttgta tcataatgaa
     6181 ctgacttctt ccattcagca tcagcaacgg gatgaatgtg acaagaaacg cagaacttat
     6241 tggttgaggt tatatccacc gcccgctggg caccaagtat gacaagcgcc ccggcaatca
     6301 gccccggggt gaaccataac atgagtttct tcataaattt aaaaatgtgc gggtcaagat
     6361 aaaaaataat ctcattcgta aaactgaata aatcatgatt taaaaggaaa aagtgaattt
     6421 ttaaaattca ggacgaatcc cggcaagcat ttcttatttt tacgatttct gacaagaaga
     6481 tataacacac atgactgaca aaaattcaaa tatgaatgaa aataatcgtg ttcaatcatt
     6541 tatgattccc tgccgcggga gtcagacagg catacgaaca aaaacaatta attattttta
     6601 tatgaaaccc acatgataca aaatcacaaa caagcatgaa aataaaatcc attttggact
     6661 ccatgtgaat cacttaacat tcagtagtat ataaaattct tctcacatga aaaagtattt
     6721 ttttattgcc atattcctaa ttctgaattt caccctgctg gctcatccgt ggaaaccatc
     6781 acattatgta ataatcgata ccgacggagg aatagacgat atgagggcaa taacaatgtt
     6841 cctggcatcg cccgatgtaa gggttctggc tgttatcgcc tcatccggca ccctttccgc
     6901 tgaaaatacc tacaggaagg taaggagcct gctgaactcc tattttcatg aaggggtacc
     6961 ggtgggaata aacagcagtg taagtttcaa atcaccagat atcagcatgg cactgaaaac
     7021 acagtggggt gatgaaacaa acctgtcacc gcaggatgca ccggcggccc tggatctaat
     7081 cagctgggtc ttttccgagg aaaagaccca aatatcattt gtttcgcttg gaggactgac
     7141 aactgcatgg acggcattta acgcgatccc gtcgttcagc cggcaggtga aagagattgt
     7201 ttggagtgct gatggatttt cggataccaa aggattcaat tacaatattg ataaaaaggc
     7261 tgcggcaggt atactgaaag ggagtattcc cgtaaaaatc ataaagccgg ccggtgaaaa
     7321 ggagttttat aacactgaac tgattgcgga tatatccggg atatgcagta tctatgcaga
     7381 aaagttatct gtttttttca gttctgcaac tgatgccgga catgatttta tatatgccgc
     7441 gaccgatgaa acgatccctc tttttattca ttttccggcg ctgttcaata caactgcctc
     7501 cggtattaat tcagaatccc gggcagctga tataaatcaa ctgagggaaa agaccctggc
     7561 aattctgaaa ggagaaacag ttgaacggaa ccaggtgata aaggaatttc cagataatcc
     7621 ggcattctat ttcgacgatc tgaaggatca tgtttcagaa attataaaaa aatacggtat
     7681 tgatgaatgg agttcaggtg tattggccaa cgaacttcac cggcatctag gagtgttcgc
     7741 cattataggc gtcaaaatgg gaattagggc aagggaatat ttcaacaccg gtgtcgatga
     7801 attcaggacc acatcctatg ccgggtccat taccccggta agctgtttca acgacggtct
     7861 gcaggttagc actggatcca ctcccgggca tggattgctg aaggtggtcg acgatgtacc
     7921 ggcaaaacca atggcggaat tcagttatat gaacaggaag atcagactca ccctgaggcc
     7981 tgatattgcc gaaaagatca tctccgaact gaaagagatc aattttattt acgggctcga
     8041 cagtaatatt tactgggagc ttgtaaggaa gaacaccatc aggtactggc tcagtctcga
     8101 caggcacgag atatttgaaa ttgaggaaat aatttagttt tactccttat tgtactcgtc
     8161 aaagaagtca ttgcccttgt cgtcggtaat gatgaatgcc gccatgttct ccacccttat
     8221 ttttctcacg gcttccattc ccagatcccc gaaatcgacc agttcgaccg atttaatatt
     8281 ctcctcggcc agaatggctg cggggccgcc gatcgatccc aggtaaaatc cgccgtactt
     8341 cctgcacgaa tcgatcacct gtctgttccg gttgcctttg gctatcatta taagggagcc
     8401 tcccatgctc tggaaaaggt ccacgtagct gtccatcctg cctgctgtgg ttggcccaaa
     8461 actccccgaa ggcttgccgg caggtgtttt ggccggaccg gcataataaa cagggtggtt
     8521 tttgaagtat ttcggcatgg gttgtccttc atctatcatt ttctttatcc gggcatgagc
     8581 tatgtcgcgg gcaactatca atgttccgcg aaggttcagc cgggttctga cgggatacct
     8641 ggtaagctcg gcaagtattt ccttcattgg acggtcgagg tccagctcaa cgggtttctc
     8701 aagttccggt gccttttctg gcaggaaacg tgccgggtcc ttttccagct cttccataaa
     8761 aataccattc gcgtctatcc ttgccctgat attcctgtcg gcgctgcaac tgaccccaag
     8821 tccaacggga caggatgcag cgtggcgggg aagtcgtata accctgacat catgcacgaa
     8881 atatttgcct ccgaactggg cccctacccc gtattcgtga caaatctttt ctatcctttg
     8941 ttcccaggcg atatccctga atgcccgtcc ggcctcgttc ccggtcaccg gaagatcatc
     9001 gagatagcct gcagaggctt ccttcacagc cttcagcgtt gcttccgctg aagtaccgcc
     9061 gatcaccagc gcaagatgat acggagggca tgctgaagtg cccagatcct taattttttc
     9121 cctgacaaat ttttccagcg attcctcatt cagcaacgat ttgctctcct gataaaggta
     9181 agtcttgtta gccgatcctc ctccctttgt tatgaaaagg aactcgtata cattaccttc
     9241 cgttgcgtaa atatcaatct gggcaggaag atttgttccg gaattctttt cttcgaacat
     9301 ggtgtagggc acaatctggg aataccggag attcctatcc tgaaatgtcc tgtaaatacc
     9361 tttcgaaaga tgcagggcat cattggctcc ggtatagaca tcctctccct ttttccccat
     9421 cacaattgcg gtgccggtgt cctggcacca tggcagttct ccgcgcgctg atattaccga
     9481 gttacggatc attacatagg ccacgaacct gtcattatct gttgcctcag gatcatcaat
     9541 gattttcctg agtttttcga tgtgtgatgt cctcaggtaa aaggaaagat ccgaaaccgc
     9601 catggcggca aggagttcaa gtccctccgg atccaccttg agaacttttc tgccacccga
     9661 ttcgacaatt tttacaaaat cacctgccag tttacgatac ctggttgaat cccctccggt
     9721 ctgaaagggt ttttcataaa taaaatcggc catatctctg ttattcaatt aacaatgaat
     9781 ttattgcagg atactgcaga atctttgtca aagataggaa caaaacggga atgatattct
     9841 ccaaagtttt ccacaaccgt gaacaatccc gggcgctaaa gttctgacaa cacagttgcc
     9901 gcataccgtt ctccagtcct ggtgcgggta ttttggaaga tgcttatcaa atgcatatgg
     9961 gattttggtg gcctgtgcag gtcagggtat aatttctccg ggtgaattta ttacatcaat
    10021 acgggttttt aagaataata tccctgtcca ggcaaaccta gcggaggagt tatttacgga
    10081 ggatctcact gaaactgaac tttcttccgg gcctgatccc tttgggcgtc atttccaatg
    10141 tacaacattc cgtaagttcg tcatgctgaa atacaattat gtggttcttg tccagagcct
    10201 cattcagtaa cctgtttttt tcctcgatgg tcctgagggt ttcaatatca taactcatgt
    10261 tccacaatag cggaacatgg gccgcggttg gtataaggtc gcccgcgaac acaatggttt
    10321 tgccgttata atcaatcatt gcatacatca ggccgggggt gtgcccatag cagatcctga
    10381 ctgaaaatcc cctgaacagc tcacaatcct catcgattaa gttaagccgg ccgccatgca
    10441 tcagtggaag aatattttcc tcaaggaagg aatcctcttc gcgcggattg tttttcacag
    10501 cccattccca ttgaacacgg ctcacatgga gggcagcatt tgggaacacc atcttggttg
    10561 tccggccatc attgctcagc cgtgttgcgc ctccgcaatg gtcagcatgc agatgtgtga
    10621 aaacaacatc ggtaatatcg tttcccgaat agcccagcct gcgaaggcct tcaataaggc
    10681 cttcgccccc actcagattc aggtgtctga aaaattttgc atcctgtttg tcaccccagc
    10741 cggtgtcaac aagtataaca tgaccgtcgg tttccgcaac aatcgatctg agcgccagaa
    10801 caataaaatt attttcatcg ggaacacaga cccttgacca taaaaccctg ggaataactc
    10861 cgaacattgc tccgccatca accttgaaat tagtgatatt gaaaagatta agcttcattt
    10921 atcccggttt gaacacaata atggcaaaaa taattatttg acccgggaaa aatccgattc
    10981 atgtgttatg gcagtaaaaa agtttgttac agtgattatc ggggcctgtc aatttgaaaa
    11041 aatcgattat ttttagggct gaataataag ctatcccgtt ttccagtaag aaaaaacaaa
    11101 aatctgactg tatgaaaaag ttattgcttc cagtgattat atttttattc ccacaggtta
    11161 tgccagctca gaccggaaaa gtcatggaca atatgtcgat gccaagcaag atattaaaaa
    11221 tggacaggaa atttgccgtt tacctgccac ccgactatga aacctctcaa agggcttacc
    11281 cggtactgta tctgttgcat ggttacggtg atgatcagac cggttgggtg cagttcggag
    11341 aggttcagca tataactgac aaggcaataa atgaaggcaa ggcaacgcct atggtaataa
    11401 taatgcccga tgcaaacacc gggaaggtgg gttatgtcaa tgatatcaag ggagagtggc
    11461 gatatgagga tttctttttt gatgagctga tgccatacgt tgaaaagatt ttccgcataa
    11521 aaggtgaaaa gaattaccgt gcaattgccg ggttatcgat gggaggagaa ggaactttca
    11581 tttatgcgct ccatcgtccg gaattgtttt cttctgcctg ccccctcagc gccgcaaccg
    11641 gtccggaaaa tattgaggag acgggtcagt acatactgtg gcctgccgta aaaagaatat
    11701 ccggtgccga aagggataag tatttcaata attacagtgt cctgagtctg ataaacaata
    11761 ttcaggaaaa aaacaagaaa gccgtaagat ggtacatcga ctgcggtgat gatgatttcc
    11821 tctttgaggg aaattcgctt gtccacatag caatgcggaa gaaggagatt ccccatgagt
    11881 tcaggatccg cgacggcggc cacacctggt cgtactggag aagcgctttg ccatcggttc
    11941 ttgaattcat ttcgatgagc ttccatcagc actgaaaaac agtttatccg gagttttcag
    12001 ttagacggct tctttttcct ggaagtcatc tgcgccatta tgcgcatgaa gagcctgatc
    12061 atctgatacg acatccatcc cctgagctgc gaataccagg tcctgcgcct cagatattct
    12121 tcaaatgtta tctgcctgca atcgttcctg ataatttccg aaagaagatc ctcgaatttc
    12181 cgggtaaaag ccgtgtcagt cacacccacg ttcatttcta tgctcccgta gtcgctgata
    12241 tgattcagat tataactgcc aatagtgcac cattttccgt cgacagcggc cacctttgca
    12301 tgaaggttgg caggcaggta ttcataaatc gtgatattgt tccttaaaat atattcataa
    12361 agaaaacgtg ttgcacgctt gaacatggga gcatcagatt ctgccgtgag gactattttt
    12421 atatccacac ccctcgtgct tgcctgcctc agcagtttcc tttcgaggcg ccccggaaga
    12481 aagtaactgg caagaatgat catcctcaaa tgggcatgtt tgaatgcatg ccggtaactc
    12541 ctgagtattt ctatcttgtt gcggtaccag ttattttcga gaaccctgag cctgatattc
    12601 tcttcatagg ttttgatctg gctggcagtc tcatgagccc gctcttgcct ggaaataaat
    12661 gttttgttcc aatgcctcct gagaatactg tttacgtgca cgcactcagg tcctttcatc
    12721 aaaacagcaa aatcgagcca ttcttttttt tgcggagttc cgtgatagcg gtcagcatag
    12781 ttcatccctc cgattattgc agcttcgcca tcaacgagaa gtaccttgtg atgaaggcgg
    12841 agactgagct ggaaaccttt cttgataaaa accggggaga aaaacctgaa aaggatcccg
    12901 gagctttcaa tccttttgat caacccaccg gaaaggtatt ttgtaccgaa agcatcgaga
    12961 agcagatata tcctgactcc cctttcagcg gctctgatca gtgcgtcgat cactctcagg
    13021 ccagtctgat cctcatcaac aatgtaggtt tggaaatgta tatatcgttt ggcctcattt
    13081 atcagcctga cattctcggc aaaaaactgc tcaccgctcc gcaacagttc cagtgagtga
    13141 cccccgatat agtcgcgctt cagtcttgta tccataaaac agctcctttc atggtacatt
    13201 gtacaaatat aagcgtttaa ccggaaggct cctattggat gttcctgcaa cttttcacag
    13261 tacccgcggc ccacctccgc taaattccgt tcctgccaca cggattgtaa taacaaaaag
    13321 caacgggttc cgttatacct ctacatttca gcattcccgg atgttgccgg caaattcagg
    13381 caacctggga tgatgggtta cattatttcg ttaatgaaaa gaggtttttt aatcgtcctg
    13441 ctgagtctga tccttgcatc atcctcgtac agccaggaat ataaaaccag tatgggtata
    13501 agggcaggcc ttcccattgg actgactctt aagcattttc tgaacaggga ttatgcattg
    13561 gagggtatgc tggctaccag atggggcggc attacacttg caggtttcct tgaaaaggaa
    13621 caccgggtaa aaaatcacca cggcttatac tggttctggg gaggcggaat ccattcaggg
    13681 atctgggatg ccggttacaa ccccaggctg aaggccggtt atacgggccc ggtttttgga
    13741 attgacggga taataggact tgaatacaca ctggactacc atcccctgaa tttctcattc
    13801 gacctgtatc cctcattgaa tcttatcgga tcagcaggat ggggaggaat aaacggtgcc
    13861 gtgtcagtca gatacgtttt ttaatgaaaa attttgtata tcaaaaaaaa cattgtttct
    13921 ttgcatccgc atttaaaagt tcttttaacg gtccattcgt ctagtggtta ggacgtcagg
    13981 ttttcatcct ggtaacag
//
LOCUS       DAOOYG010000068        13805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4498_length_13805_cov_6.339709, whole genome shotgun sequence.
ACCESSION   DAOOYG010000068 DAOOYG010000000
VERSION     DAOOYG010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4498_length_13805_cov_6.339709"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PK288_07170"
     CDS             complement(<1..785)
                     /locus_tag="PK288_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18741.1"
                     /translation="MKKLLVFFVLINCCTGIILAQGEIDKQQRVFLRNERSFGLLLSS
                     DGIGIGYREAKRVDYFNKRFFEIEAGTLKHPREHKMTNPIYQTPGTFVFGKLNSVMYL
                     RGSYGHQRELFQKADLGGVAIRYFYEGGPVIAIYKPIYYKILYPISSYEFAIREEKID
                     VNTHQPYDVYSKASFIKGFGETKLLPGLYAKGGFNFEYSKEDKVIHAIEIGAQINGFP
                     KKIPIMATTDNKAIFFSLFVSYRFGVVIDPLNPETNKFSYIFRR"
     gene            1160..2044
                     /locus_tag="PK288_07175"
     CDS             1160..2044
                     /locus_tag="PK288_07175"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HPM18742.1"
                     /translation="MGTDLFSQGRLPYNDLTLQESRVIVNKGTEIPFTGEYTDHYENG
                     TYICKRCGAALYYSSSKFRSDCGWPSFDDEIKGAVNRFPDPDGRRTEIVCSSCGAHLG
                     HVFTGEHNTSKNIRHCVNSVSLDFVPVHLQPGRYGTAIFAGGCFWGVEYFLQKAEGVY
                     SAISGYTGGHVRNPSYREVCTGTTGHAEAVKVIYDPVKTSYEKLLKLFLEIHDPTQVG
                     RQGPDIGEQYRSEIFYLNDEQKEAAERNIAVLKGKGIKVVTAVTKATEFFPAEEYHQN
                     YYFNNGKTPYCHVYTKRF"
     gene            complement(2073..3206)
                     /locus_tag="PK288_07180"
     CDS             complement(2073..3206)
                     /locus_tag="PK288_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18743.1"
                     /translation="MEKIRLGMIGGGPGAFIGDAHRRASRICNDYELVGGVFSSNSEK
                     NTQFAVNEGIDLSRCYSGVESLIEAEKALPANKRMEAVAIVTPNALHYSAARMLLENG
                     FHIVCEKPMTMTVEEALELEEIVNQTKLTFALAHTYTGYPMVRQMRDLISQGILGTIQ
                     RIDAQYYQGWINSFIHGTESRITGVWRLDPRHAGVSSCMGDIGVHAFNLIEYTTGLEI
                     REILSDLSRVKDNIKLDLDGTVLLRFGGYLKGVIRASQVCGGEENGITIAVYGSRASL
                     KWAQENPNYLYMLSDTEPTRVFKPAHDYNGKFAEISHTMPPGHPEGIYEALANIYRGA
                     AKSIRGEEFIPGEFPTVHEGVRGMKFIHAVVNSDANGNVWTKV"
     gene            complement(3289..4281)
                     /locus_tag="PK288_07185"
     CDS             complement(3289..4281)
                     /locus_tag="PK288_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005646997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPM18744.1"
                     /translation="MSRPVTLFTGQWADLPLETVCAKAVSFGYDGLELACWGDHFEVD
                     KATDEYCMKKRELLDRHHLKVFSISTHLVGQAVADRIDERHKGILPEHVWGDGDPEGV
                     RERAAREVIKTGEAAKRLGVSVVNGFTGSPIWHLCYSFPPVPAAMIENGFREFARRWK
                     PILDAYRELGIRYALEVHPTEIAFDIASARRALEAVDNHPAFGFNYDPSHFGYQGVDY
                     VKFIYEFRDRIFHVHVKDAGWSDTPADAGVFGGHTEFGTLGRFWDFRSPGRGNINFEE
                     IIRALNRIGYKGPLSVEWEDSGMDREHGAAEACEFVKSIDFAPSDIAFDSAFEK"
     gene            4421..5167
                     /locus_tag="PK288_07190"
     CDS             4421..5167
                     /locus_tag="PK288_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPM18745.1"
                     /translation="MQDILLNRRTIRKYSLEPVDDNLLVELLKAGCRSSTTGNMQVYS
                     IIINRDESLKKALAPLHFNQAMVTEAPVLLTFCADFNRFNRWCLLRKAVPGYDNFISF
                     MTAAIDALLVAQTLCVAAEARGLGICYLGTTTYMAHKIIEVLRLPKGVVPVTCISLGW
                     PAEKPQQVDRLPLEAVIHNETYKDYTDEEIEKLYCEKENRSDSMQFIEENKKETLAQV
                     FTDIRYKKEDNVYFSKVLLQVLHDQGFMNQ"
     gene            complement(5371..6441)
                     /locus_tag="PK288_07195"
     CDS             complement(5371..6441)
                     /locus_tag="PK288_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPM18746.1"
                     /translation="MYTKAFLYDFTTMVFMKMGCSEADAGIIADVFLAAESRGHPSHG
                     MIRLRDYFELWKAGRINMNPDIRIVHESPSTAVVNGDNAVGMIPARRSMEIAIEKARA
                     AGTGWVATFNSNHFGIAGYYSMMALSRDMIGICLTNANPLVAPTFSVSRLLGTNPIAV
                     AVPALHQPPLIADFATTPVARGKLAIAEKKGEKVAFGFVQDKDGNPSDDPAILKSGGS
                     MLTLGGDRLHGSHKGYCLSALVDIFSALLSGANFGPFVPPSVAYLPILEKKTGEGTGH
                     FFGAMRIDAFQPANAFKAKMDEWIATFRSAKTATGEERVIIPGDPEREAEERTSREGI
                     AIVPAVRNDLKAIAAELGLEFE"
     gene            complement(6580..7338)
                     /locus_tag="PK288_07200"
     CDS             complement(6580..7338)
                     /locus_tag="PK288_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM18747.1"
                     /translation="MNGIVMVTGATAGFGRAIAVRFASEGYGVIITGRRKDRLDELER
                     ELTRSGAKVLPLNFDVRSKTEVEHVLNRIPDAWKEVGILVNNAGLAAGLSHIDQGDTD
                     DWERMIDTNVKGLLYVTRTIVPVMIARGGGHIINISSIAGSDIYENGNVYCATKSAVN
                     SLSRAMRIDLLGHNIKVTNIAPGMAETEFSLVRFKGDREKAKAVYKGVDPLTAADIAD
                     IVYFCATLPAHVCINDLTVTPTQQAGSGFVYRRG"
     gene            7463..8209
                     /gene="ubiE"
                     /locus_tag="PK288_07205"
     CDS             7463..8209
                     /gene="ubiE"
                     /locus_tag="PK288_07205"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HPM18748.1"
                     /translation="MKDHLHRKDESVSGDKTAAIRKMFDSIAWRYDFLNHFLSFNIDR
                     YWRRRVSVIIREHGSFSRILDVATGTCDLAIAVARLNPEKITGIDISERMLEIGQAKI
                     DRRGLSGLIELMKCDSENICFSDNSFDVAMVAFGVRNFTDPLEGLKEMHRVLRRGGLL
                     IVLEFSKPGGFLFKRIYNLYFRYLLPFLGQLFSKDKGAYRYLNESVMKFPDNEQFLAI
                     MKSAGLSDLKQLKLTFGIVSIYTGLKVQKQ"
     gene            8267..9019
                     /locus_tag="PK288_07210"
     CDS             8267..9019
                     /locus_tag="PK288_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPM18749.1"
                     /translation="MNRRNFYFLLAGLLLIFQVDAFPQKQKPKNESWYDEKPLHFGFT
                     LGMNLMDFNITPADSLKAPDFLNPEVSKLNPGINIQIVTNLRINSHADLRFLPGVSFG
                     QRNIRYYKNKSGTGTNNGNPEMEIYNERQRLESSFLEFPLIFKYKGERLNNVRPYVIG
                     GLNYRYDLAGKKEYDDEKAVYLRLKRSDLYYEAGAGLDFYLPYFKLSVEIKMSNGLRD
                     VIVHDPAPGYPQFVNAIEKVKSQIWILSFHFE"
     gene            complement(9062..9841)
                     /locus_tag="PK288_07215"
     CDS             complement(9062..9841)
                     /locus_tag="PK288_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPM18750.1"
                     /translation="MISKSKINYIKSLRTKKARIEESSYIIEGDKLIKEYIMSGKVIR
                     LLVAKSGFIESLTDEQRALIDDIETVNERGLERISSLSTPHNALAVVPITTVKTDPGT
                     ILEKLCAAIDCIQDPGNLGTIIRSAAWFGITDIICSPDSVDSYNPKVIQSAMGALMHV
                     NVHYCRLETFLGEAVRKNVPVYGALLEGKPVYSMKLRNTGVILLGNESRGISNELLEF
                     VTEKMAIPRFVSFRYGINSLNVSMAATVIFSEFARRRGSRQ"
     gene            9947..12307
                     /locus_tag="PK288_07220"
     CDS             9947..12307
                     /locus_tag="PK288_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPM18751.1"
                     /translation="MDKTLKERTSNNNAKFPGRSLLPLFLTLLSIYSCNPTKYIPEGQ
                     TLLDKNHISLTRDGLKKSELQPYIRQKPNKKIFGARFHLGLYNLSNIKKEKWPHAWLR
                     NIGEEPVIYDPDATERSREQIRSYIASKGYFDSSVEDSVRVVKRKSEVYYNVDLKTPY
                     TIRNLYYEFSDTTIRKLFNFDSVNCIIERGKPYDVDVLQAERTRFERFIKDRGYYGFS
                     SENIYFRVDSTIGNRQVNIYYGIRNYLRLDDSGKPVYVPHSVYRIKNIYIFPDFVPKE
                     VLEGGESYQQSLDTMLYKNYYFVTPRKKSDLKYDLILQAIYLKPGFVYNVTNTEQTQN
                     HLMALRTFRHVNIFFREEGTVKDGRDELALNCFIQLTLLDQQSYKIELEGTNTTGDLG
                     GALNLVYQHKNLFHGAELFNLKLKGAYEAISQSDTVRKLKSTQEYGVETSLRFPKFLL
                     PVLKAERFVKKYSPSTTVVAAYNYQKMPSYTRTMANASFGYNWNAGPYKTHIVNPLQM
                     NLINLLWIDEKFKAKIDTTSYLSYSYKDVMILGSNYSFIFNNQKIQKSRDYWFLRVNA
                     ETSGNLLALISSMSGLQKKDGSYNIFGQPFAQYVKADMDLRYNVILNEASSIVYRGFL
                     GVGIPYGNSKALPFERQYFGGGANGIRAWPARSLGPGSYLHQEKGFVNQTADIKLEAN
                     AEYRFKLFWILEGALFLDAGNIWTYNEDESRPGSKFVFKYLLDDIALGTGTGLRFDFK
                     FVIGRLDMGMKLRDPSIRGGPNWIMANRKYNFRNDFTFVLGIGYPF"
     gene            12406..13062
                     /locus_tag="PK288_07225"
     CDS             12406..13062
                     /locus_tag="PK288_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPM18752.1"
                     /translation="MNSFREFVRSWFGYTRRERRASFILLIIVILISLFRLAIPKHTV
                     SIEMMALDIKNPAADPVLMAEQQSPVKKTGDERKFNRKIPLLDINTSDSSALVTLPGI
                     GPVLSMRIIRYRNLLGGFWSVNQLKEIYGLPEETFVKISSRLYADTLKIRKIKINAAD
                     YREMIRHPYFRKEEVQAIIKYRKLEGFIKDIEDLYHNNILTRETLQKIKPYIDYSGQP
                     "
     gene            13336..13527
                     /gene="rpsU"
                     /locus_tag="PK288_07230"
     CDS             13336..13527
                     /gene="rpsU"
                     /locus_tag="PK288_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPM18753.1"
                     /translation="MIIVPLKEGENIERALKKFKRKFEKTGVIRELRARQAFIKPSVR
                     RREEIKKARYVQKMQERED"
BASE COUNT         3833 a         3097 c         3038 g         3837 t
ORIGIN      
        1 cgtcggaaga tgtaggaaaa tttattggtc tcggggttga ggggatcaat taccacccca
       61 aatcggtaac tgacgaataa tgagaagaat attgctttgt tgtcggtcgt tgccattatc
      121 ggtatctttt tgggaaatcc gttgatctgg gcaccgattt caatggcatg aattacctta
      181 tcctccttgc tgtattcaaa attgaatccg cccttggcat aaagaccggg aaggagtttt
      241 gtttcaccga agccctttat gaatgaagct ttactgtata cgtcataagg ctgatgtgta
      301 tttacatcga ttttttcttc cctgatcgca aattcatagg atgatatcgg gtacagtatt
      361 ttgtagtata tgggtttgta tattgcaatt acgggcccgc cttcatagaa ataccttatt
      421 gcaacgcctc caaggtcagc tttctggaac agttcgcgct ggtgcccgta acttcccctc
      481 aggtacatca ccgagttaag ctttccgaaa acaaatgtgc cgggagtctg atagatagga
      541 tttgtcattt tgtgttccct gggatgtttc agggtgccgg cttctatctc aaaaaagcgc
      601 ttgttgaagt aatcaacccg ttttgcttcg cggtaaccga ttccgatccc gtcggagctg
      661 agcaggagcc caaatgacct ttcattacgt aaaaacaccc tctgctgctt atcaatttct
      721 ccctgtgcca gtattatacc ggtacaacaa tttataagca caaagaatac aagcagcttc
      781 ttcattaatc atttgaatga ataagtggag atcctgctag atccggcagc actcagacat
      841 tatcttcagc ctgctcagtt tcatttttgg agtatgccgg acaagctttc tcattacatg
      901 agctgacaaa tattgcagtt ataaaacata acacaagcaa aacagcaatc tttttcataa
      961 aaccatgtaa tttattagtt cacaaaatta agatattcac tttaaaaaca atctttgtgc
     1021 caaattatta ttttaaggct catcattttg ttctttaacc gtaatttcaa aacataatta
     1081 ttataaaatt gttgtaccgg aggaattaaa tatttcagaa atgaaattga ggacatggat
     1141 catattttat cttttattta tgggaacaga tttgttttct cagggcagac tgccttataa
     1201 tgatcttaca ttacaggagt caagggttat tgtaaacaag ggaacagaga ttccttttac
     1261 cggggagtat accgaccatt atgaaaacgg aacctatata tgcaaaagat gcggggctgc
     1321 tctttattat tcatcatcca agttccgctc agactgcggc tggcccagtt tcgacgatga
     1381 gataaagggg gccgtgaaca ggtttcccga tcccgacggc aggaggacgg aaattgtatg
     1441 ctcatcgtgc ggggcgcatt tgggtcatgt tttcacagga gaacacaata cctcaaagaa
     1501 tataaggcac tgcgtgaatt ccgtttcact cgattttgtg cctgtccatt tgcaacccgg
     1561 aaggtacggc accgcaattt ttgcgggcgg atgtttctgg ggagtggaat attttctgca
     1621 gaaggctgaa ggtgtttatt cagctatatc cggttacacg ggtggacatg tcaggaatcc
     1681 ctcctacagg gaagtctgta cgggaacgac tggtcatgct gaagctgtta aggttattta
     1741 tgatccggtc aaaacttcat atgaaaaact gctgaagctt tttctggaaa ttcatgatcc
     1801 gacccaggtc ggcaggcagg ggccggacat tggtgaacag tacaggtcag aaatattcta
     1861 tctcaacgac gaacaaaagg aagcagctga aaggaatatt gctgttctga aaggaaaagg
     1921 gatcaaagtt gttactgcag tgacaaaagc cacagagttt ttccctgccg aggaatacca
     1981 tcagaattat tatttcaaca acggaaaaac tccatattgc cacgtatata caaagcgatt
     2041 ctgactggct gcagaaaagt gtttcctcgt cattacactt ttgtccatac gtttccgttg
     2101 gcatcagagt taacaaccgc atggatgaat ttcattccgc gaactccctc atgcaccgtg
     2161 gggaattctc ccggaatgaa ttcttcaccc ctgattgact tggcagcacc cctgtaaata
     2221 tttgccagag cctcgtagat tccttcagga tgccccggag gcattgtatg gctgatttcc
     2281 gcgaattttc cgttgtagtc atgagcaggt ttgaagaccc tggtcggttc cgtgtcgctc
     2341 agcatataca gatagttcgg attttcctgt gcccatttga gacttgccct ggatccataa
     2401 actgcaattg taataccgtt ttcctcgccg ccgcatacct ggctcgccct gataacaccc
     2461 ttgagatagc cgccgaaccg gagcagaact gttccgtcga gatcgagctt gatattatcc
     2521 ttcaccctgc tcagatccga aagtatttcc cttatttcca ggccggttgt atattcaatc
     2581 aggttaaatg cgtggactcc gatgtctccc atacagctgc ttacccctgc atgccggggg
     2641 tctagtctcc aaacccctgt tatcctgctt tctgtgccgt gaatgaacga attgatccat
     2701 ccctggtaat actgtgcatc tattctctgg attgtaccca gtatgccctg ggaaattaag
     2761 tcgcgcatct ggcggaccat gggataccct gtataggtgt gtgccagagc aaaagtaagt
     2821 ttggtctggt ttacaatctc ttcgagctca agggcttcct ctaccgtcat tgtcataggt
     2881 ttttcacaca cgatatggaa tccgttctca agaagcatcc tggcagcgga atagtgcagc
     2941 gcatttggag tcacaatggc aactgcttcc attcttttgt tggcaggcag ggctttttca
     3001 gcttcaataa gtgattcaac gccactgtag catcttgaga gatcaatacc ctcatttacg
     3061 gcaaattgag tgtttttttc gctgttggag ctgaatactc cgccaacaag ttcataatcg
     3121 ttgcatatgc gcgaggctct ccggtgagca tctcctataa aagcaccggg acctccgccg
     3181 atcattccca atctaatttt ttccatgtta tacggttata aaaagtttgc ttcaataata
     3241 caatctgcag gtaaggatgg tttaaatatt accgggtgag caggatcatt atttttcaaa
     3301 tgctgaatca aatgcaatgt ctgacggtgc aaagtcgatc gatttaacaa attcgcatgc
     3361 ttcagcagcg ccgtgttccc ggtccatacc actgtcttcc cattcaaccg acaagggccc
     3421 cttgtaacct atcctgttca gtgccctgat aatttcctcg aagtttatat tgccgcgtcc
     3481 cggactcctg aaatcccaga accttcctaa ggttccgaat tctgtatgtc ccccgaaaac
     3541 gcccgcatca gcgggagtat ctgaccagcc ggcatctttt acatgaacat gaaatatcct
     3601 gtcgcggaat tcataaatga atttcacata atcaactccc tggtatccga agtgcgacgg
     3661 atcgtaattg aacccgaagg cggggtggtt atcaacagct tccaatgctc tccttgctga
     3721 tgcaatatcg aatgcaatct cggtcggatg cacttccagg gcgtacctga taccgagttc
     3781 gcggtatgcg tccaggatgg gtttccaccg gcgtgcaaat tccctgaagc cgttttcaat
     3841 catagccgcc ggaacaggcg gaaatgagta gcagagatgc cagattgggc ttcctgtgaa
     3901 accattaaca acagaaactc ccagcctttt tgccgcttca cccgttttta tcacttcccg
     3961 ggcagcccgc tcacggactc cttcaggatc tccgtcgccc caaacatgtt caggaaggat
     4021 ccctttatgc ctctcatcaa tgcggtctgc cactgcctga ccaacaaggt gtgttgatat
     4081 tgagaacact tttaaatgat gtctgtcaag caactccctt tttttcatgc aatattcgtc
     4141 tgtagccttg tcaacctcaa aatgatcgcc ccagcatgca agctcaagac cgtcatatcc
     4201 gaacgaaaca gccttggcac aaacagtttc aagtggcaaa tcggcccact ggcccgtgaa
     4261 taatgttact ggtctgctca tagtattgtt gttttgattt gattgaacct gattcaatgg
     4321 ttcatgctaa gataagaaaa tccttttcat aagctcttcc gcttccttca gaatttgctt
     4381 actttgtgta taatcttcaa attaattgtc tatggctgat atacaggata tacttctgaa
     4441 ccgcagaacc atcagaaaat acagtctgga accggttgat gacaatcttc tcgtagaact
     4501 gctgaaggcg ggctgccgtt catcaacaac gggtaacatg caggtctaca gtataataat
     4561 caacagggat gagtccctga aaaaagcttt ggcaccattg catttcaatc aggcaatggt
     4621 aaccgaagcc cccgtgttgc ttactttctg cgcagatttc aacaggttta acagatggtg
     4681 tctgctcaga aaggcggtcc cgggttatga taatttcatc tcattcatga cggctgccat
     4741 tgatgcattg ctggttgccc agacgctttg tgtcgcggca gaagccaggg gactaggcat
     4801 atgctatctt ggcactacaa cctatatggc acacaaaatc atcgaagtgc tcaggctgcc
     4861 gaaaggagtt gtgccagtaa catgcatatc cctcggttgg cctgcagaaa aacctcaaca
     4921 ggttgacagg cttccccttg aagctgttat acataatgaa acatacaagg attatactga
     4981 cgaggaaatc gaaaaattat actgtgaaaa ggaaaacaga tctgattcca tgcaattcat
     5041 agaggaaaat aaaaaagaaa cactggcaca ggtcttcacc gatatccggt ataaaaagga
     5101 agataatgtg tatttttcaa aagtcctatt acaggttctc catgaccagg gatttatgaa
     5161 tcaataagag tttgaaagac ccgattcccc gaaacgggaa atgaagaaat acaagatcaa
     5221 cttattacac tgccggattc ttattctccc ccgttgcggg gatggatgcg cagtatgcct
     5281 ttacctaagc cccggtttta cctttaaaga ccggcagatc attacaccag tattggaatt
     5341 ggccggaacg ggagccttca caggaagact ttactcaaat tcaagtccaa gctccgccgc
     5401 aatcgccttc agatcatttc tcacagccgg aacaattgca atgccttccc ttgaggtcct
     5461 ttcctcagct tccctttccg gatctcccgg aatgatgaca cgttcctctc ctgtagcggt
     5521 tttggctgac ctgaatgttg ctatccattc atccattttc gccttgaagg catttgcagg
     5581 ctgaaatgca tcgatcctca ttgctccgaa aaaatgtccg gttccctcac ctgttttttt
     5641 ctcgaggata ggaaggtatg ccacactggg agggacgaac ggaccaaaat tggctccgct
     5701 cagaagtgcg gaaaaaatat cgacaagtgc cgacaggcag taacccttat gactgccgtg
     5761 gagcctgtcg cctccgagag tgagcatcga tcctcccgat tttagtattg caggatcatc
     5821 cgagggatta ccatccttat cctgaacaaa accaaatgca accttttctc cttttttctc
     5881 agctatggca agttttcccc tggcaacagg ggttgtggca aaatcggcta tcaggggggg
     5941 ctgatgcaac gcgggtacag ccaccgcgat cggattggtg cccagaagcc tgctgaccga
     6001 aaaggtaggc gcaacaagag gattggcatt ggtaagacag atgccgatca tatccctgct
     6061 cagggccatc atcgaataat acccggcaat cccgaaatga ttggaattaa atgttgctac
     6121 ccatcctgta ccggctgccc gggccttttc gatggcaatc tccatcgatc tcctggccgg
     6181 aatcatgcca actgcgttat caccattgac caccgcggta ctgggtgatt catgaacgat
     6241 ccttatatca gggttcatat ttatccttcc ggctttccat aactcaaaat aatcccttag
     6301 cctaatcatt ccgtgagacg gatgccccct cgactcggcg gcaaggaaaa catcggcaat
     6361 gatgccggca tcagcctcgc tgcaccccat cttcatgaaa accatcgtgg tgaagtcata
     6421 aagaaatgcc tttgtataca tgatctgctg cctgtttttt atcccttaaa tcccgggact
     6481 tgatttcgtt ttattttgaa ctgtattgaa tatactccgt accagggttt gacagccgga
     6541 cggctaatcc ctgcctgcgg catgaactgt cctgtcctgt catccccgcc tgtatacaaa
     6601 tcctgagcct gcctgctggg tgggagttac cgtaagatca tttatacata catgtgccgg
     6661 cagggttgca cagaaataaa cgatatcggc tatatctgca gctgtaagcg gatcaactcc
     6721 tttataaacc gccttagctt tttcccggtc gcctttgaag cggacaagtg agaattctgt
     6781 ttctgccatc cccggggcaa tgttggtgac cttgatgtta tgcccgagca aatcaattct
     6841 catcgccctg gagagtgaat tgacggcact ttttgttgca cagtaaacgt tgccgttctc
     6901 ataaatatca cttccggcta ttgaggaaat atttataata tgcccgccgc ccctggcaat
     6961 catcacaggt actatcgtgc gggtgacgta caataatccc ttgacgtttg tatctatcat
     7021 cctttcccag tcatccgtat ctccctggtc aatgtgagat aatcctgcgg caagaccggc
     7081 attgttgacc aggatgccga cctccttcca ggcgtccgga atacggttca gaacatgttc
     7141 gacctcggtt ttactcctta catcaaaatt gagcggcagc acttttgctc ctgaacgggt
     7201 gagttccctt tcaagttcgt caagccggtc tttccggcgt ccggtaatga tcacaccgta
     7261 accctccgat gcaaatctga cagcaattgc tctcccaaaa ccggcagtgg caccggttac
     7321 cataacaatt ccattcatcc tgcgtgattt gatttccgat gtaaaaataa cataaaaaaa
     7381 cacaaatatg cggactgttg cagtctgaac aatatgaact gacctgattc aaattgtgca
     7441 tatatttgca tccggatgta aaatgaaaga tcacttgcat agaaaggacg aatccgtgtc
     7501 cggggataaa actgctgcca tccggaagat gttcgattca attgcgtgga gatatgattt
     7561 tctgaatcat ttcctgtcct ttaatatcga tcgctactgg agaagacggg tatccgtgat
     7621 catcagggaa catggctcat tttcccggat ccttgatgtc gccaccggta cctgcgacct
     7681 tgccattgcc gtggcgaggc ttaatccgga aaaaataacc ggaattgata tttcagaacg
     7741 gatgctggaa atcgggcagg caaaaattga caggcggggt ttgtcgggcc tgatcgaact
     7801 aatgaagtgt gattcggaaa acatctgttt cagcgataac agctttgatg ttgcaatggt
     7861 ggctttcggg gtcaggaact tcactgatcc tctcgaaggg ctgaaggaaa tgcaccgtgt
     7921 ccttcgcagg ggaggattgc tcattgtgct tgaattctca aaacccggcg gattcctgtt
     7981 taaaaggatt tataatttgt atttcagata cctgcttcca ttcctcgggc aattattctc
     8041 aaaggataaa ggggcttaca ggtatctgaa cgaatcagta atgaaattcc ctgacaacga
     8101 gcaattcctt gcaataatga aaagtgccgg actgtcagat ctgaagcagc tgaaactgac
     8161 attcggcatt gtaagtattt ataccggact taaggttcag aaacaataat tgaaaagtaa
     8221 tcgagtttaa taaattggcg ccaatataaa accaggatca gcaattttga acagaagaaa
     8281 tttttatttc ctgcttgccg gattattgct aatatttcag gttgatgcat ttccccagaa
     8341 gcagaaacct aaaaatgaat cgtggtacga tgaaaagccg cttcattttg gttttactct
     8401 gggtatgaac ctgatggatt ttaatattac acctgctgac agtctgaaag caccagattt
     8461 tctgaatcct gaggtcagca aactgaatcc cgggatcaat attcagattg tgacaaacct
     8521 gcgcataaac agtcatgctg acctgagatt cctgccagga gtatcattcg ggcaacggaa
     8581 catcaggtat tataaaaata aatcaggtac tggaacgaat aacgggaatc ctgaaatgga
     8641 gatctacaat gagcgtcagc gtcttgaatc ttcattcctg gaatttcccc tgatattcaa
     8701 gtacaagggt gaacgtctta acaatgtgag accttatgtt ataggaggtt tgaattatcg
     8761 ttacgatctt gccggtaaaa aagaatatga cgatgaaaag gcggtttatc tcaggctcaa
     8821 gcgttcggat ctttattatg aagctggcgc agggcttgat ttctacctgc cctatttcaa
     8881 gctttcagtt gaaattaaaa tgtccaacgg attgagggat gtcattgttc acgatcctgc
     8941 gccgggctat ccccagtttg taaatgcaat tgagaaggtt aagtcacaga tatggatcct
     9001 gtcgttccat tttgaatagc gatactgaaa cttgtctggc cctgcagttt gttttataaa
     9061 ttcattgcct gctgcctctc cttctggcaa attccgaaaa aatcactgtg gcggccatgc
     9121 tgacattcaa tgaattgatc ccataacgga aactcacgaa tctcgggatt gccatttttt
     9181 ctgtaacaaa ttccagcagt tcgtttgaaa tccccctgga ttcattgccg agaagaatca
     9241 ctccggtgtt ccggagtttc atcgagtaaa caggcttgcc ctcaagcagt gctccatata
     9301 cggggacatt ttttctgacg gcttccccga gaaaggtttc cagcctgcag tagtgcacgt
     9361 ttacatgcat gagtgcaccc atggccgact gaataacctt gggattgtaa ctgtcaacgg
     9421 aatccgggga gcagataatg tccgtaatgc cgaaccaggc ggcggaacgt attattgttc
     9481 ccaggttacc cggatcctga atgcaatcaa tggcagcaca tagcttttca aggatcgtgc
     9541 ctggatcagt tttgacggtt gtgatgggta ctaccgcaag ggcattatgc ggggtagaaa
     9601 gggagcttat tctctccagg ccccgttcat tcaccgtttc aatatcatct atgagtgccc
     9661 tttgctcatc cgtcagcgat tcaatgaacc cggattttgc aacaagcagc ctgattactt
     9721 tgcccgacat gatgtattcc ttaataagct tgtccccttc aatgatatat gatgattcct
     9781 caatcctggc ctttttagtc cgcaatgatt ttatgtaatt tattttgctt ttgctgatca
     9841 ttccagatga ttttagattt tatacatcat aaaaatagcg agttatttca ttatgaaggg
     9901 atcgtgcccg tcttatttgt tataataagt atatttgcct gatgctttgg ataagacttt
     9961 gaaagaaagg acttcaaata ataatgctaa attcccggga agatcgcttc ttccattatt
    10021 cctgacctta ttatcgatat attcctgcaa tcctacaaaa tatattcctg aaggacagac
    10081 ccttcttgac aagaaccata tttcccttac cagggatggt ttgaaaaaga gtgagcttca
    10141 accttatatc aggcagaagc cgaacaagaa gatattcgga gccaggtttc acctgggttt
    10201 atataatctg tccaatatca aaaaggaaaa gtggcctcat gcctggctga ggaacatcgg
    10261 agaagaaccg gttatctatg atcccgacgc aacagagaga agccgggagc agatccggtc
    10321 atatattgcc tccaaaggat atttcgacag cagtgttgaa gacagtgtca gggttgtgaa
    10381 aagaaaatcg gaggtttact ataatgtgga tcttaaaact ccatatacca tccggaacct
    10441 gtattatgaa ttttccgata caactatcag gaaactattt aattttgatt cggttaactg
    10501 tattatagag cggggaaaac cctatgatgt tgacgttttg caggctgagc gcacaaggtt
    10561 tgaacgattc ataaaagatc ggggttatta tgggttttca agcgaaaata tctatttcag
    10621 ggtcgacagt acgattggca acaggcaggt aaacatctat tacggtatca ggaattacct
    10681 ccgtcttgat gactccggga aaccggttta tgttccgcac tctgtttacc ggataaaaaa
    10741 catctatatt ttccctgatt ttgtaccgaa ggaagttctc gaaggaggtg agtcgtatca
    10801 gcagagcctg gatacaatgc tttacaaaaa ttactacttt gttacacccc ggaaaaaatc
    10861 cgatcttaaa tatgacctga ttctccaggc aatatacctg aaacccggat ttgtctataa
    10921 cgtcaccaat acggaacaga cgcagaacca tctgatggct ttgagaactt tcaggcatgt
    10981 aaatattttt ttcagggagg aaggaacggt taaagatggg agggatgaac tggccctgaa
    11041 ctgttttatt cagctgacgc ttctcgacca gcagtcatac aagatcgagc ttgaaggaac
    11101 caatactacc ggtgatcttg gcggagcact taacctggtc tatcagcata agaacctatt
    11161 tcacggcgct gaactgttca atcttaaact gaagggggca tatgaagcca tttcccagag
    11221 cgacacggtc agaaaactaa aaagcacaca ggagtatggc gttgaaacga gtctgaggtt
    11281 tcccaaattc ctccttccgg tgcttaaagc cgaacgcttt gttaagaaat acagtccatc
    11341 aacaacggtt gttgctgctt acaattatca gaagatgcct tcctataccc gtaccatggc
    11401 aaatgcatct tttggttata actggaatgc agggccctac aagacgcata tagtcaatcc
    11461 gcttcagatg aacctcatca acctgctgtg gatagatgaa aagttcaagg caaagattga
    11521 cactacatcc tatctttcct atagttataa ggatgtgatg atactcggat ccaactactc
    11581 ttttattttc aataatcaga aaatacagaa atcaagggat tactggttcc tcagggtgaa
    11641 cgccgaaaca tcgggaaatc tgctggcgtt gataagcagt atgagcggac tgcagaaaaa
    11701 ggatgggagc tacaacattt tcggacagcc ttttgcccag tatgttaaag ccgatatgga
    11761 cctgaggtat aatgtgatcc tgaacgaggc gagctcaatt gtttacaggg gatttctcgg
    11821 ggtaggcata ccatacggaa attcaaaggc gcttcctttt gagaggcaat atttcggcgg
    11881 cggggcgaac ggtataagag catggccggc aagatcactc gggccgggtt catatctcca
    11941 ccaggaaaag ggcttcgtga accagaccgc cgatattaaa cttgaagcga acgcggaata
    12001 caggtttaaa ctgttttgga tcctggaagg agcacttttc cttgatgcgg gaaatatctg
    12061 gacctataat gaagatgaat cccgaccagg ttcaaagttt gtgttcaaat atcttctcga
    12121 tgatattgcc ctgggtaccg gtacggggct gaggttcgac ttcaaattcg ttattgggag
    12181 gcttgacatg ggaatgaaac ttcgcgatcc atccatacgc ggtggaccaa actggatcat
    12241 ggctaaccgg aaatacaatt tcaggaacga ctttaccttt gtccttggca taggataccc
    12301 attctgatgc attttaatgg attattgaaa taaagtaagc agaagacgct taatattccg
    12361 cgaagttttc taaattgcaa ttgtcgagct gctttaactg caatcatgaa ttcattcagg
    12421 gaatttgtcc ggagctggtt cggttatacc cggcgggaac gaagggctag ttttatttta
    12481 ttgattattg taatactgat ctcacttttc agactggcca tacccaaaca tactgtttca
    12541 attgaaatga tggcccttga tataaaaaat cctgctgctg acccggtcct aatggctgaa
    12601 cagcaaagcc ctgtgaaaaa aaccggtgat gaacgaaagt tcaacaggaa aatccccctg
    12661 cttgatatca acacaagcga ctcatcagcc ctggtcacgt tacccgggat cgggccggtg
    12721 ctttcgatgc ggattatcag gtacagaaac ttgctcgggg gcttctggtc ggtaaatcag
    12781 ctgaaagaga tatatgggct accggaggag actttcgtga aaatatcttc aagattatat
    12841 gccgacacgt taaaaataag aaagataaaa ataaatgccg ctgattaccg ggaaatgatc
    12901 agacacccgt atttcaggaa ggaagaagtg caggccataa tcaaatacag gaaactggaa
    12961 gggttcataa aagacattga ggacctgtat cacaataata tactcaccag ggaaacactg
    13021 cagaagatta aaccctacat agattattcg ggacagccat gaagtgacca ggagattcgc
    13081 ctggactgct ttccgccctc gttttggcag attggtttaa aaaagcccgg ttttatcaat
    13141 aattagcctc atattttttg gaagagaccg gggttttgcc tatctttatt acatccgtag
    13201 ataacattgt ttatgttcat ccgatgtaat attcaataat acagttaaga atgagaaaat
    13261 aattaaagga aatattttga aaaaactttt tcagtatcta cctttgcgcc tcatttgaaa
    13321 ccagaaaatt aaactatgat tattgtacca ttaaaagaag gcgaaaatat tgaaagagct
    13381 ttaaagaagt tcaagaggaa attcgaaaag acaggcgtta tccgtgaatt acgtgcacgc
    13441 caggcgttta ttaagccttc agttcgccgg agggaagaaa tcaagaaggc aagatatgtt
    13501 cagaaaatgc aggaaaggga agattagttt ccgttccatc tttacgtaat atatccaaac
    13561 cgataaggcg gttcctttat ggatcataag gaatcattct tgcagtatct tcagattgaa
    13621 aagcgttatt ctccgcttac cgtaacatca tatctgaatg atttaaatca gtttcacagg
    13681 tttctgtcag aacaggccat ccattcggat cctgccggga caacatccca tgatatcaga
    13741 tcctggatag tgagcctggc agagtcagaa tattcatcgc ggagcatcca taggaaaatt
    13801 tcttc
//
LOCUS       DAOOYG010000069        13290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4761_length_13290_cov_7.120740, whole genome shotgun sequence.
ACCESSION   DAOOYG010000069 DAOOYG010000000
VERSION     DAOOYG010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13290
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4761_length_13290_cov_7.120740"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..779)
                     /gene="hypB"
                     /locus_tag="PK288_07235"
     CDS             complement(117..779)
                     /gene="hypB"
                     /locus_tag="PK288_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPM18754.1"
                     /translation="MRNIELNTDILASNNIIASMNRRFFEGRKTFCLNLVSSPGSGKT
                     TILEKTIDIIGKNRKIYVIEGDQQTLIDAERIGRTGVPVLQINTGSGCHLDAGMIESA
                     LKKMDPEPDSILFIENVGNLVCPALFDLGEYRRVLIVSATEGDDKPLKYPLMFESSQL
                     CLINKSDLLPYVDFSTDDFIRNARSINPGLEFITLSAKTGEGMDAWYRWISEKSVLFA
                     GK"
     gene            complement(776..952)
                     /locus_tag="PK288_07240"
     CDS             complement(776..952)
                     /locus_tag="PK288_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18755.1"
                     /translation="MNMIIFMAVIILMFIPIPMTMIITIMTMIITMVTSMRTVMDTIT
                     RMTMKTTITRGRTG"
     gene            complement(1071..1418)
                     /gene="hypA"
                     /locus_tag="PK288_07245"
     CDS             complement(1071..1418)
                     /gene="hypA"
                     /locus_tag="PK288_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPM18756.1"
                     /translation="MHEFSLAAEVLKIAGKEAGKNNARAVNEITIEIGNMSGVEISTF
                     RSALEMLSEGSILDKTRLNLIMIKGKGFCRRCSLEFEMEHRTDTCPACSTFPEEIRNG
                     HEFRIVSLLIEEE"
     gene            1536..2924
                     /locus_tag="PK288_07250"
     CDS             1536..2924
                     /locus_tag="PK288_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPM18757.1"
                     /translation="MEDQRSLIRTLGLGYVILFVVANIVGSGVFKKIAPMAAELQSSS
                     WVLLAWIAGGIVTLFGALSNAEVAGLLADTGGDFVYFKKIYNRFFAFLYGWSLFTVIQ
                     TATIASLAWVFAQSLNSIVPVPEVLGSLKDFTLGGIFYPFLDFGIKLVAIVLILLLTG
                     LNISGLKSGAGFSRAILVLVGLGLLVIIISGLASGPDRQADLLNAGDITSGSVSLSAF
                     YTAMLAAFWAYQGWVSVGFIGGEVLEPKKNIPRGIVIGVFIVILIYVVVNTTYLSILS
                     IPRLIQIHESQNQIAAVEAVRSYAGTTGAVLISVLILVTTVSCANASILTGARPYFAM
                     AREGLFIPGIGKINRNNVPGTSLLLQGIWASVLVLSGTFDQLTDMIIFTVYIFYGAIA
                     LGVFILRRRMPDAFRPYRVWGYPVVPALFILFCIGLFLNTIITRPREAAIGLTLVVAG
                     IPVYMLMNRKQK"
     gene            3024..4016
                     /locus_tag="PK288_07255"
     CDS             3024..4016
                     /locus_tag="PK288_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPM18758.1"
                     /translation="MSHYLHSLLAGLVTFLFVTGSLNAARYVTIATIGNVPSVERNLQ
                     PEKMVDQVIAFWRNELNQVLPDKPDLILLPEFCDLSGAGEEYLNARKDRVLDFFASVA
                     RDHRCYIVFGTKRMNPEGIWRNSCIILDRGGMIAGIYDKNFPTIGEMEQGIKASDEAP
                     VFKCDFGRVGIAICFDLNFDELRLKYAAQKPDMILFSSMYHGGFVQSLWAYSCRSFFV
                     GSVYRSTPSEILNPLGEVIASNTNYFDYAVAKINLDNEIVHLDYNWDKLKELKNKYGI
                     GVNIHDPGKLGVVLVTSNNEKVTVQEMIDEFRLERVDDYFQRARKFRLNVGNLR"
     gene            4101..4385
                     /locus_tag="PK288_07260"
     CDS             4101..4385
                     /locus_tag="PK288_07260"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HPM18759.1"
                     /translation="MILAGLGALSLSREKAEEISRDLIKRGELAETDEAKFVRDLMDL
                     VEKNKAGLEEKVEKAVEKAMAKLDIPSRKEINALKEEIGKLTGKDKVSGE"
     gene            4450..6099
                     /locus_tag="PK288_07265"
     CDS             4450..6099
                     /locus_tag="PK288_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HPM18760.1"
                     /translation="MQHFKNLDKLRRTRQIAGVLTRYGLGYLIDRQRKGFFLTGQKDK
                     VKLSAPQKFCRALEELGPTFIKFGQILSTRPDFLPPEFIKELERLRDHTPPFKVSEAL
                     QIIEKELGRPADKIFASFDPVPVAAASLSQVHKAALPNGDDVAVKIQRPGIRQIIETD
                     LAILQDLAGFAEDRLHNGWLYRPRLMIEEYRKAVRKELDFTREAQNFEKFRINFRNTE
                     YIRVPNICWELTTSTVLTMEFIEGIKINETVREEYRDVYDPRKVAERGVEAVMKQILE
                     DGFFHADPHPANLFVMPPAEIVMLDAGMVGYLDRNTRFYGAKLLQALVNRDAESTLNR
                     FEDLKIIISDVDRSFLCQELTELFDSYLGMPIGNLDISKAGQEVIGIMIRHNLTIPPN
                     LVLMIKALSMIEATGKELDPDLNILEIAGPFVRNLSRKRLDPREILKKSNAILQESVE
                     LIERLPEDINLILNKIREGKLKFVFEFQEAEKLKKAACTAGHQISLSIIIAALVIGSS
                     VIFALQENSPVIFGFPASGIAGYFLALILAVIFLWSVLRHR"
     gene            6129..6947
                     /locus_tag="PK288_07270"
     CDS             6129..6947
                     /locus_tag="PK288_07270"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM18761.1"
                     /translation="MIIDGHAHLGGDYRDVKSIVSVLDRLGVDKVVLCPADSIRRQSM
                     SLPDMGTRVSGMKLNFVVNRFIRSVSATRSKWAAIEMANKKIFDTAALSGGRVLQFYW
                     VNPLETTADKLEEKLELWKFAGIKLHQGCHPFRIKSGIFRDIAGFAALKSLPLFIHLY
                     SRKEVMDFISVSGNFSTNFIVGHLIGLEIFIKQRKMLGDNVFFDISCPPLVSSERIRL
                     AINEFGVRKIIMGSDTPYGRDNLERVLSRIRSLELKTEEIDMILGNNLEKILLI"
     gene            complement(6978..8438)
                     /locus_tag="PK288_07275"
     CDS             complement(6978..8438)
                     /locus_tag="PK288_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPM18762.1"
                     /translation="MRPAAKSTGTRKYFRHCFCIRKAALLTLLKVFFISGLQSQPSEF
                     TRTVSCEEASAILSEYIRYPSVTGNEKPAGQYIEGICREKGLHVRVFTDSTDSYNFAA
                     SLYPLAMNKPNIIFLNHIDVVQEGDDSGWVYPPFSGTITGDTVWGRGAADMKGAAVMQ
                     LMAIASFAGDAAESDFPVNATLLCVSGEEKYGKTGAGIISSSFLGELNPAVVFGEGGI
                     GARNLIKKAPEKPVFAISLSDKRALWLKLRLINKTSGHGSIPPPEYANKIMINALHSL
                     VNSRTRIRFSSPVRTMFRNYGKLEKGITRLVLGKPGLFKPVIAGVVKKDPLLLSSVTN
                     TITITRFRGQENEVNQIPQEVEVMLDCRLLPETPTDEFINLLGRKLKNNDIEISVLIE
                     TQNAAPTVPDKYYDMFCESLDEAYPGSGILPVLFPATTDNNYFRSAGIPVYGIIPAIF
                     SEEEMKSIHNFNERISVGALEKGTEVYMRFLGKLLR"
     gene            9090..9692
                     /locus_tag="PK288_07280"
     CDS             9090..9692
                     /locus_tag="PK288_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HPM18763.1"
                     /translation="MSSVAFDNRLSSGSGIWFFLIAELILFGLLFIFYLVYRITDAQA
                     FHFAAGELSVFSGTVGAVILLVSSAVLAGSVTAVKQEKKRLALQLLGMTFLLGIIFLV
                     ARCLEWRVHIKAQIFPGSPVLALRGPGDLLFYGFYFFITGLHALHVIIGLAVIGFLFR
                     GFSRGKIGSGDYPAYEKGVWYWHLVVLLGILIFPVFYLIT"
     gene            complement(9896..11074)
                     /locus_tag="PK288_07285"
     CDS             complement(9896..11074)
                     /locus_tag="PK288_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18764.1"
                     /translation="MKEQFQVSTTFRIIILILMAIGACTFVIGLFTDRETTWAGYLVT
                     NYYFFSIAMGGVFFYLLQSITQSGWSSAFKRVPEAMMSYIPVAALFFLLLYFGTHDLY
                     RWSDPEAAAVDPLIRSRAVFMNVPFFYIRIALFFALWIIFIRILRKLSLREDSFDPAD
                     HEGIMALFAKSELYSKIFIFILAITFTLSAFDLVMSIDAHWYSTIFAFKNLVAAFLHG
                     VSVMILIIFILHRRGYFPFLNKYHLHDFARYLFMLSILWGYFWFAQFMLIWFGNIPEE
                     TVYYYVRWQDGWKVMFFLEIGLNWAIPFMVLLPIKTSRNMMVITMVIAFLIIGQYIDL
                     FVQIIPGTTGVLKFGLIHAGLFVGYAALFALVVATALGKAKIIAPNHPYLEESLKHKF
                     "
     gene            complement(11127..11735)
                     /locus_tag="PK288_07290"
     CDS             complement(11127..11735)
                     /locus_tag="PK288_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPM18765.1"
                     /translation="MIHYNIKMASRRSIVLIILLMLLNSCSRDRHTTGWDYFPDMFYS
                     IAYETYSPNPNFADGMTMREPVPGTVPRGYIPFEYTIELESRIEAGKELRNPLLPTAD
                     ALARGKEEYTIFCSGCHGVLGDGKGFLFTSGLYPLQPKSLTDSVARNLKEGEIFHTIT
                     LGRGFMGAHGAQVAPDDRWKIILYIRQMQEETLQTAGAGREE"
     gene            complement(11732..12265)
                     /locus_tag="PK288_07295"
     CDS             complement(11732..12265)
                     /locus_tag="PK288_07295"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HPM18766.1"
                     /translation="MARTFKIGIFDDEEKFISSLKRLKEQKHSVYEVFTPYPVNEVLH
                     LLKRRTRLPTAAYFFGLFGIVATLMFLYYTSVISWPVVYGGKPFNSFPSFIVVTIVVT
                     ILTVTILSLALFSGRSKLYPGRDNTIFDGRATDDMFIIVMDTEALDEQSAEEAGEIMR
                     TEGAIEIINKEIETVYT"
     gene            complement(12277..>13290)
                     /gene="nrfD"
                     /locus_tag="PK288_07300"
     CDS             complement(12277..>13290)
                     /gene="nrfD"
                     /locus_tag="PK288_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPM18767.1"
                     /translation="VLAVACAALFPLIHMGRIWLAFYIIPYPNTRGPLWVNFNSPLFW
                     DFTAITAYLLISVSFWYLGMLPDFGTIRDKTSSKIRKAVYGFFSLGWTGSSRQWIRFE
                     SLSFVFGGLAAVLVVSVHSIVATDFAAGIVPGWHSTVFPPFFVVGAIFSGFAMVMTLM
                     VIIRKIYRLTDYITDNHMDAIARILVFISLIMAVSYLVELFIGWYSGNEYEKYAVIHN
                     RVAGEYAPQFWGMILCNALIPQLFWFRKFRRNWVSLLLISLVINIGMWLERYNIVVTP
                     LSKDFLPSSWVTYKPTFIDIGVFVGTIGLFATGVLLFMRYIPMMAISELKGVVNIGNK
                     EED"
BASE COUNT         3497 a         2950 c         3217 g         3626 t
ORIGIN      
        1 aacagggtta cggaagtacc tttgcttaaa aggggcagaa atcagtcgtt cgatatagat
       61 gccggttaca ggaccctgtg gtacataatc gtcaggtagc agcagttgca cagaggttat
      121 ttcccggcaa aaagtacaga cttttcgctg atccatctgt accatgcatc cattccttca
      181 cctgtttttg ccgacagggt gatgaattca agacccggat ttatgctccg cgcattccgg
      241 atgaaatcat cggtgctgaa atcaacatag ggcaggaggt ccgacttgtt tataaggcat
      301 agttgggatg actcgaacat taacggatac ttcaggggct tgtcgtcgcc ttccgtagca
      361 ctcacaatca gcaccctccg gtattcgccc aggtcgaaca aggcaggaca gacaaggttc
      421 ccgacattct caatgaaaag gattgagtca ggttcgggat ccattttttt aagcgccgat
      481 tcaatcattc ctgcatcgag atgacagccc gaacccgtgt ttatctgaag aacaggaacc
      541 cctgtcctgc cgatgcgttc agcgtcgatg agtgtctgct gatcgccttc aatgacataa
      601 attttcctgt tttttccaat aatgtcaatg gtcttttcaa ggatggtggt cttgccggat
      661 cccggagaac ttaccagatt caggcagaaa gtttttctcc cttcaaaaaa ccttcggttc
      721 atggaggcga taatattgtt tgacgcaaga atgtcagtat tgagttcgat atttctcagc
      781 ctgtgcgtcc cctggttatg gtggtcttca tggtcatgcg ggtgatcgtg tccatgactg
      841 tgcgcatgga ggtcaccatg gtgatgatca tggtcatgat ggtaatgatc atggtcatgg
      901 ggatgggaat gaacatgagt atgatcactg ccatgaagat gatcatgttc atgtccggtc
      961 ttttctgcct gggaagacaa tggatccgtt attacaaatc cttccgtctt gccgcaaccg
     1021 catgaatcac acatattcgg attttttatg atttatcagc atggtgcatt ttattcttct
     1081 tcaatcagca gggaaacaat cctgaattca tggccattcc tgatctcttc ggggaatgtg
     1141 ctgcaggcag gacatgtatc ggtgcgatgt tccatttcaa actcaaggct gcatctccgg
     1201 caaaaccctt tacccttaat catgatcaga ttcagtctgg tcttatctaa tatcgaacct
     1261 tccgaaagca tttccagggc agaccggaag gtgctgattt caaccccgct catgttgccg
     1321 atctcgattg ttatttcatt cacagcacgg gcattgtttt ttcctgcttc tttcccggca
     1381 attttcaata cttcagcggc cagtgaaaac tcatgcatga tgtacatttt gcatatccaa
     1441 tttagccttt ttttttcagg cagcaaaaaa aatatgatgc agaaacccat gaaaaggaat
     1501 catagcttat tttcgggaaa taattcttca tcgggatgga agatcagagg agccttataa
     1561 ggacgctcgg ccttggatat gtgatcttat ttgttgttgc caacatagta ggttcaggag
     1621 tatttaagaa gatcgcacca atggcagccg aactccagtc ttcctcgtgg gtgctgcttg
     1681 catggatagc aggcggaata gtcaccttgt tcggtgcgct cagcaatgcc gaggtggccg
     1741 gtcttcttgc agataccggc ggcgattttg tttatttcaa aaaaatatac aaccgtttct
     1801 ttgcatttct ttacggatgg tcgctgttta cggtgatcca gactgccacg atagcatcgc
     1861 ttgcatgggt ttttgctcaa tcgctgaaca gcattgttcc cgttccggaa gtactcggtt
     1921 cattgaagga tttcaccctt gggggaattt tttatccgtt ccttgatttc gggataaagc
     1981 ttgttgcaat tgtgctgatc ctgctgctga caggattgaa catttccgga ctgaagagcg
     2041 gggccggctt cagcagggcc atccttgttc tggtgggcct cggactgctg gtgatcatta
     2101 tctccgggct ggcctcggga ccggaccgcc aggctgatct tctgaatgcc ggtgatatca
     2161 cttcaggcag cgtttccctt tcagctttct atacggcgat gcttgccgca ttctgggcat
     2221 accagggatg ggtttccgtt ggattcatcg gtggggaggt attggagccg aaaaagaata
     2281 tacccagggg aatagttatc ggagtattta ttgtgattct gatctatgtt gtggttaaca
     2341 ccacctatct gtctatcctt tcaatccccc ggctcatcca gatacatgaa tcgcagaatc
     2401 agattgctgc agttgaagct gtcaggagct atgcagggac tacaggagct gtcctcattt
     2461 ctgttcttat tcttgtaacc acggttagtt gtgcaaatgc cagtatactg acaggtgccc
     2521 gtccatattt cgccatggcc agggaaggcc tgttcatccc gggcatcgga aagataaaca
     2581 ggaacaacgt tcctggtaca tcacttctgc tgcaggggat atgggcatcc gtcctggtcc
     2641 tgtcaggaac attcgatcag ctgactgaca tgatcatttt taccgtttat atcttttacg
     2701 gagcaatagc cctcggcgta tttatcctgc gccgcaggat gcccgacgcc ttccggccct
     2761 acagggtatg gggatatccg gttgtaccgg cactcttcat attgttctgc ataggtcttt
     2821 tcttaaacac aataattacg cgtccccgcg aggcagcaat aggcttgact cttgtcgttg
     2881 ccggcatacc ggtttatatg ctgatgaaca ggaaacagaa ataaccgtct gctgcccggg
     2941 ctgctatcct atcttccaat gtgagcagcc gcgtgcctca ttcttcagga tttcttatct
     3001 ttgattaaac aatagctgaa cagatgtccc attatttaca ttcactcctg gccggactgg
     3061 taacatttct gtttgtcacg ggcagcctca acgcggcaag gtatgttacg attgcaacca
     3121 ttggcaatgt tccctctgtt gaaaggaacc tgcagcctga aaaaatggtc gaccaggtga
     3181 tcgcgttctg gaggaatgag cttaatcagg ttcttcccga caagccagat ctgatcctgt
     3241 taccggagtt ctgcgatctt tcaggcgcag gagaggaata tcttaatgcc agaaaggaca
     3301 gggttctaga tttttttgca tcggttgcca gggatcacag atgttatatt gtttttggga
     3361 cgaaaagaat gaatcctgaa gggatatggc gcaattcatg tataatactt gatcgtggcg
     3421 gcatgattgc aggaatttat gacaagaatt tccctacgat aggagaaatg gaacagggga
     3481 taaaagcatc tgatgaggct cctgtcttta agtgcgactt tgggagggta gggattgcaa
     3541 tctgttttga ccttaacttt gacgaattga ggttgaaata tgcggcccaa aaaccagata
     3601 tgatcctgtt ttcctccatg taccacggcg gttttgtcca gagcctgtgg gcctattcgt
     3661 gcaggtcgtt ttttgtggga tcggtttaca ggtcaactcc gtcggagatc cttaacccat
     3721 tgggcgaagt gattgcatca aacaccaact attttgacta tgctgtcgca aaaataaacc
     3781 ttgataatga aattgttcat cttgattaca actgggataa actcaaagag ttaaaaaata
     3841 aatacggtat tggtgttaat atccatgatc ccgggaaact gggcgtggta cttgtgacaa
     3901 gcaataatga aaaagtcacc gtacaggaaa tgatcgatga gttcaggctt gaaagggttg
     3961 atgattattt ccagcgtgca aggaagttca ggctgaatgt cgggaatctg aggtagcaga
     4021 aatctgtggc agtaataaaa tgtagcatta tcgtttgaaa accaaataat atagaaccca
     4081 tgacagattt gatcaaaaaa atgattttag cgggtctcgg agctttatca ctcagccgtg
     4141 aaaaagctga agagatatcg agggatctga taaagagagg agaactggca gaaacggatg
     4201 aagcgaaatt tgtcagggat ctgatggatc ttgttgagaa gaacaaagcc gggttggaag
     4261 aaaaggttga aaaggcagtg gaaaaagcca tggcaaagct tgatatacca tcccggaagg
     4321 agatcaatgc cctgaaggaa gagattggca aactaaccgg gaaggacaaa gtttcagggg
     4381 aatagaaata cattgtcctt ctgtcctttt ttccatggat tgttcttcag ctttagccgt
     4441 aagaccatta tgcaacattt taagaacctg gataagctaa ggcgcaccag gcagatagcc
     4501 ggggttctta caaggtatgg cctgggatat cttattgacc ggcagaggaa agggtttttt
     4561 ttgaccggac aaaaagacaa ggtaaaactg agtgctcccc aaaagttttg cagggccctg
     4621 gaggagctgg gtcccacgtt tataaagttc gggcagatac tcagcacacg tcccgacttt
     4681 ttgcctcctg aattcataaa ggaactggaa agactccggg accatactcc cccctttaag
     4741 gtatctgagg ctttacagat tattgagaag gaactcggaa ggcctgcaga taagattttt
     4801 gccagttttg atcctgtccc ggtggcagct gcctccctga gccaggtcca taaggctgct
     4861 cttcccaatg gtgatgatgt ggctgtcaag atccagagac ccggaataag gcagataatt
     4921 gaaaccgatc ttgcaatcct gcaggatctt gcaggatttg ccgaggatcg ccttcacaac
     4981 ggatggcttt acaggccccg tttaatgatt gaagagtaca ggaaggctgt aagaaaagaa
     5041 cttgacttta cacgggaggc tcagaatttt gaaaaattca ggataaattt caggaatacc
     5101 gaatacattc gtgttccaaa tatctgctgg gagcttacca catcgacggt gctgaccatg
     5161 gagttcattg aagggataaa aataaatgag acggtccggg aagagtacag ggatgtttat
     5221 gatccgcgca aggttgcaga aagaggagtc gaggctgtga tgaagcagat actcgaagat
     5281 ggcttttttc atgcggaccc ccatccggcc aaccttttcg tcatgcctcc ggctgaaata
     5341 gtaatgcttg acgcgggaat ggtaggatac cttgacagga ataccagatt ctacggggcg
     5401 aaattgctcc aggctcttgt aaacagggac gcagaaagca ccctgaacag atttgaagac
     5461 ctgaaaataa taatcagcga cgttgaccgc agcttccttt gccaggaact taccgaactg
     5521 ttcgacagtt atcttggaat gcctatcggg aacctcgata taagcaaagc cggtcaggaa
     5581 gtaataggta tcatgatccg gcataacctg accattccgc caaacctggt gcttatgatc
     5641 aaagcactct caatgattga agcaacaggc aaggaactgg atccggacct taacattctg
     5701 gaaattgccg gaccatttgt caggaattta tcacggaaac gacttgatcc ccgggaaatt
     5761 ctaaagaaaa gcaatgccat acttcaggaa agcgttgagc tgattgagcg gttgccagag
     5821 gacataaatt tgattctaaa caaaatacgg gaaggcaaat taaaatttgt attcgaattc
     5881 caggaagctg aaaagctaaa aaaagcggct tgcacagccg gtcaccagat atcactcagt
     5941 attatcattg cggcccttgt tatcggttct tcagttatct ttgccttgca ggaaaacagc
     6001 cccgtcatct ttggattccc ggcatctggg attgccggct attttctggc actgatcctg
     6061 gctgtgatat ttttatggtc agtgctgcgg caccggtaat ttaaggaata ataatccatc
     6121 agtctgaaat gattattgac ggacacgcac atctgggcgg tgattacagg gatgtaaaaa
     6181 gtattgtatc tgtcctggac aggctgggcg ttgacaaggt tgttttatgc cctgctgatt
     6241 ccatacgcag gcaatcaatg tcccttcccg atatgggaac aagggtgagc ggcatgaaac
     6301 tgaattttgt tgttaacagg tttatcaggt ccgtatcggc aaccagaagc aaatgggcag
     6361 ccattgaaat ggcaaataaa aagatctttg atactgcagc tttgtcgggt ggccgcgtat
     6421 tacaattcta ttgggtcaat ccgcttgaga caacggcaga taagttggaa gaaaagctgg
     6481 aattatggaa gttcgccgga ataaagcttc accagggctg tcatcctttt cgcatcaaat
     6541 cggggatatt cagggatatt gccggctttg ctgctttgaa gagcctgcct ttattcattc
     6601 acctgtattc aagaaaggaa gtcatggact tcatttccgt gtctggtaat ttcagtacca
     6661 attttatcgt aggacatctt attggtttgg agatctttat aaagcagagg aaaatgctgg
     6721 gcgataatgt tttttttgat atttcctgtc ccccgctggt ttcttcggaa cgtataaggt
     6781 tggcgattaa tgagttcggg gtccgaaaaa taataatggg atcagacacc ccgtacggcc
     6841 gggacaatct ggagagggtg ctttcaagga taaggtcact tgaactgaag acggaagaga
     6901 ttgatatgat ccttgggaac aacctggaaa agatccttct gatctgagaa ccatgctttc
     6961 agatatttcg caggaattta ccttaaaagc tttccgagga atctcatata aacctccgtt
     7021 cccttttcaa gcgcaccgac tgaaatcctc tcattgaagt tgtggatcga tttcatctct
     7081 tcctcactga aaatggcggg aataattccg tatacgggaa tacctgcact cctgaaatag
     7141 ttgttgtcag tggtggcggg aaacaaaacg ggcaggatac ctgatcctgg gtatgcttca
     7201 tcgagcgatt cgcagaacat gtcgtaatat ttgtcgggga cggtgggcgc ggcattttgt
     7261 gtttcaatga gaacactgat ttcaatatcg ttgtttttga gtttccttcc cagcaaattg
     7321 ataaattcat ccgtcggagt ttccggcagc aggcggcaat cgagcattac ctcaacttcc
     7381 tggggtatct ggttaacctc attttcctgt cccctgaaac gggtgatggt tattgtgttc
     7441 gtgaccgacg aaagcaacag gggatccttt ttcaccacgc cggcaataac aggtttgaac
     7501 aaaccgggtt tgcctagtac cagcctggtt attccctttt ccagctttcc gtaatttctg
     7561 aacatggttc tgaccggact gctgaatcgt atcctggtac gggagttaac aagggaatga
     7621 agggcattga tcattatctt gtttgcatat tcaggcgggg gaattgaacc gtgcccggaa
     7681 gtcttgttaa tcagcctgag tttgagccac agggcccgtt tgtcgcttaa tgagatggca
     7741 aataccggct tttcaggagc tttctttatt aagtttcttg caccaatgcc tccctcgcca
     7801 aaaaccaccg ccggattcag ttccccgagg aatgaactgc tgattatacc cgcaccggtt
     7861 ttcccgtatt tctcttcacc cgaaacacac agcagggtag catttacagg aaaatccgat
     7921 tcggcggcat caccggcgaa tgaagctatt gccatcagct gcatgacagc ggctcctttc
     7981 atgtcggcgg cgccccgacc ccagacggtg tcaccggtta ttgtgccgga aaacggcgga
     8041 tacacccatc ctgagtcatc accttcctgt actacgtcaa tatgattcag gaaaatgatg
     8101 ttgggcttgt tcatcgccag gggataaagt gaggcagcaa agttgtaact gtcagtactg
     8161 tcggtgaaaa ctctgacatg caggcccttt tcacggcaaa tgccctcaat atactggccg
     8221 gccggctttt catttcctgt tactgaagga tagcggatat attcactcag gatcgctgaa
     8281 gcttcttcgc acgatactgt ccttgtgaat tctgacggtt gactttgaag accggaaatg
     8341 aaaaagactt tcagcagggt cagcaaagct gccttcctaa tgcagaaaca atgccggaaa
     8401 tatttcctgg taccggtgct tttggccgca ggtcgcatct tgctgtcgga gtttaatcaa
     8461 ttaccagtga agtaacaaaa tatcttaacg tgaattcgaa ataagaagat aatttaaaaa
     8521 cactgattgt taataaaata acaaacaatt attgataatc acatggtata caattagtta
     8581 taaaaaggat tctttccaga tttatagtgc aaacaaaaaa aggaaagaat ccatgaaaaa
     8641 tcctgaaccc aaacttattg aaactttttg tcatagagat gattttaaca gggtattcat
     8701 caactaatta caaacacatc agctcactga tgggacacgc gtgcggatca aaccctgcag
     8761 ttttaatgag agcgaggtaa tgaccattgt aatttacttc catttaatgc gttacaggga
     8821 cttcaaacat tactgtcttt ttcatgtatg cgagcacatg caggatgatt ttccggggct
     8881 tgtatcgtat aaccgttttg ttgagctaat gcagaagact attctcccgc tggctgttta
     8941 cctgaagacc cgttacatgg gtaagtgtag tggaaattca agaatttata ctttttcggc
     9001 caatgtttgt ctttggattt ggcaattaat cttttatctt tatacaatca tccggcagtt
     9061 caggctgcca catacgattg taaatggtta tgtcttcagt agcatttgac aacaggctct
     9121 catcggggtc agggatttgg ttttttctca ttgcggagct gatcctgttc ggtttgctct
     9181 ttattttcta tctggtatac cgtattactg atgcgcaggc ctttcacttt gccgccggcg
     9241 aattgtcagt tttttccggt acggtcggtg cagttatact gctggtcagc agtgctgtac
     9301 tggcagggtc ggttactgct gtcaagcagg agaagaaaag gcttgcactt cagcttctgg
     9361 gcatgacttt cctgctcggg atcattttcc ttgttgcaag atgccttgaa tggcgcgtac
     9421 acataaaagc tcagattttc cccggctccc cggtacttgc cctgcgcggc ccgggagacc
     9481 ttctcttcta tggcttctat tttttcataa ccggtttgca cgcactgcat gtcataatcg
     9541 gcctggcagt gattgggttt cttttccgcg ggttcagcag ggggaagatt gggtcaggag
     9601 attatcctgc ctatgaaaaa ggtgtatggt actggcacct ggttgtcctg ctggggatcc
     9661 tgatctttcc cgtcttttac ctgataacat aagacgccct gcggtaaagg cggctgcatt
     9721 cccactgagc tgatctttcg tttatatttg ttcccggcgg tgttgcccgg taaagggtca
     9781 gcaatggttg cccgctaatt tccggcagta acccggcggc gccggtttta cttatggcgc
     9841 tggttttact tatggtgaca gctgcaaaga ctcttctgaa gcagattgcc tgtgatcaga
     9901 acttatgctt caggctctct tccagatagg gatgattggg tgcaataatc tttgccttgc
     9961 cgagggccgt tgccaccacc agggcaaaaa gggctgcata tcccacgaag agtccggcgt
    10021 ggatcagtcc gaacttgaga accccggttg ttcccggaat gatctgaacg aaaaggtcta
    10081 tatattgacc gatgatcagg aatgctatta ccatggttat taccatcatg ttcctgctgg
    10141 ttttgatcgg gagcagaacc atgaagggga ttgcccagtt caggcctatt tcgaggaaga
    10201 acatcacttt ccagccatcc tgccaccgca catagtagta gacagtctct tccgggatat
    10261 tcccgaacca gatcagcatg aactgtgcaa accagaaata tccccacagg atggagagca
    10321 tgaacaggta cctcgcaaag tcatgcaaat gatatttgtt gagaaacggg aagtatcctc
    10381 tcctgtggag gatgaaaatg atgagtatca taacagatac accgtgcagg aatgcggcaa
    10441 ccaggttctt gaaggcaaaa attgtactgt accaatgtgc atcaattgac attaccaggt
    10501 cgaatgctga gagggtaaaa gtaatggcca gtatgaaaat aaagatttta gagtacagtt
    10561 cactctttgc aaagagtgcc ataataccct catgatctgc cgggtcaaag ctgtcttccc
    10621 tgagtgacag ctttctcagt atccgtataa aaatgatcca tagagcaaag aagagagcga
    10681 ttctgatata gaaaaaggga acattcataa agactgccct gctccggatg agcggatcca
    10741 cggctgcggc ttcaggatca gaccagcgat agaggtcatg ggtgccgaag tagagaagca
    10801 gaaaaaagag tgccgccacg gggatataag acatcattgc ttcgggtaca cgcttgaagg
    10861 ccgatgacca tcctgactgg gtgatactct ggaggagata gaagaaaacc ccgcccattg
    10921 caatcgagaa gaaatagtag ttcgtgacaa gatagccggc ccaggtggtc tcacgatccg
    10981 taaacagtcc tatgacaaag gtgcatgcac caatggccat caggatcagg attattattc
    11041 tgaaggttgt cgatacctga aattgttcct tcattgctcc catggtatta aacaattata
    11101 ttaatcgagt cgctctcctg tgaggttcac tcttctcttc cggctcctgc agtctgcagg
    11161 gtctcttcct gcatctgcct gatatagagt ataatcttcc atctgtcatc aggtgccacc
    11221 tgtgctccgt gtgcgcccat aaatccgcgg cccagtgtga tggtatgaaa tatttcaccc
    11281 tctttcaaat tccttgcaac agaatcagtg agtgattttg gctgtaatgg gtagaggccg
    11341 ctcgtaaaaa ggaatccttt tccgtctccc agaacgccat ggcatccgga acagaaaatg
    11401 gtgtattcct cctttcccct ggccaatgca tcagcagttg gcagcagcgg gttcctaagc
    11461 tctttccctg cctctatcct cgattccagc tcaattgtat actcaaaagg gatatagcca
    11521 cgcggtactg ttcccggcac aggctctctc atggtcattc cgtctgcgaa gttcggattc
    11581 ggtgaatagg tttcataggc tatggaatag aacatgtcag ggaaataatc ccatcccgta
    11641 gtgtgcctgt ccctgctgca actgttcaga agcatcaaca ggatgataag cactatcgac
    11701 ctccgggaag ccatttttat attgtagtgt atcatgtata aactgtttcg atttccttat
    11761 tgataatctc aattgccccc tctgtccgca ttatctctcc ggcttcctct gctgactgtt
    11821 catccagggc ttctgtatcc ataacaataa tgaacatatc gtcagtggcc ctgccatcga
    11881 atatcgtatt atcgcgcccc gggtagagct tcgatcttcc tgagaagagc gcaaggctca
    11941 gaatggtcac tgtcagtata gtcacgacga ttgtaactac aatgaatgag gggaatgagt
    12001 tgaatggctt gcctccatac acaaccggcc agctgatgac tgaggtgtaa tagaggaaca
    12061 tgagtgtagc cacaataccg aagaggccga agaagtaggc tgccgttggc agtcttgtcc
    12121 tgcgtttcag caggtgcagc acctcattga ccgggtaagg cgtaaagact tcataaacag
    12181 aatgcttctg ctccttcagc cgcttcagtg atgagatgaa cttctcctca tcatcgaata
    12241 ttcctatttt aaaagttctg gccataatca ggaatgttaa tcttcttctt tgttaccgat
    12301 atttacaact cccttgagtt cgctgatcgc catcattggg atatacctca tgaacagcag
    12361 caccccggtg gcaaacaggc caatagtacc tacaaatact ccgatgtcaa tgaaagtagg
    12421 tttataggtg acccaacttg aaggcaggaa gtccttgctg agcggtgtta cgactatatt
    12481 atatctttcg agccacattc caatatttat gaccagtgag atcagcagga gggagaccca
    12541 gtttcttctg aacttcctga accagaaaag ctgtgggatc agggcattac agagtatcat
    12601 tccccagaac tgcggtgcat actcaccggc aaccctgtta tggatgacag catacttctc
    12661 atattcattg cctgaatacc atccgatgaa gagttcgaca agatatgaga ctgccatgat
    12721 aagagatatg aagacaagta ttctggctat ggcatccata tgattatcgg tgatgtaatc
    12781 agtgagccga tagattttcc tgataattac cataagggtc atcaccatgg caaaccctga
    12841 aaagattgca cccaccacaa agaaaggagg gaagacggtt gaatgccagc ccggtactat
    12901 cccggcggcg aagtcagttg caacaattga gtgtaccgat accaccagta cggcagcgag
    12961 gcctccgaaa acaaagctga gcgattcaaa ccggatccac tggcggcttg agccggtcca
    13021 tccgagactg aagaatccgt aaacagcttt tctgattttt gatgatgtct tatcgcgtat
    13081 tgttccgaag tcaggcaaca ttcccaggta ccagaacgag actgatataa ggagataggc
    13141 agttattgca gtgaaatccc agaagagcgg tgagttgaaa ttgacccata gcgggcccct
    13201 ggtattcggg tatgggataa tatagaatgc cagccatatg cggcccatgt gtatcagggg
    13261 gaagagagcg gcgcaggcga cagccagtac
//
LOCUS       DAOOYG010000070        13212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4806_length_13212_cov_7.486129, whole genome shotgun sequence.
ACCESSION   DAOOYG010000070 DAOOYG010000000
VERSION     DAOOYG010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4806_length_13212_cov_7.486129"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1147
                     /locus_tag="PK288_07305"
     CDS             122..1147
                     /locus_tag="PK288_07305"
                     /inference="COORDINATES: protein motif:HMM:NF037867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5627 domain-containing protein"
                     /protein_id="HPM18768.1"
                     /translation="MKKLLIILALVTGLIACENWEQKFDDYNFTGVYFPYQYPVRTLV
                     LGDDIYPNENDNNHMCIIYAAFGGVYENKFNRVVNFTLDQSLCNSVRFGLSAGDTVRL
                     MPTTYYTLSSNSSLTIPKGEFMGGVEVHLTDAFFNDPMSVGINYVIPLRITGTSDVDS
                     VLSGRTSKESPDRRVAGDWDVLPKDFTMYAVKFVNPWHGHYLHRGVTVTKEIAPDTVV
                     NTQVQHQKFIEKDEVWTVSTSGKNKVTYSGTLRSNKLSGDLKMDLTFDANNNCVISDA
                     GSAFPVSGTGKFVKNAESWGNKDRNVIYLTYTATDGTYSLTANDTLVVRDRGTVLQTY
                     NPVILTK"
     gene            1249..3717
                     /locus_tag="PK288_07310"
     CDS             1249..3717
                     /locus_tag="PK288_07310"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HPM18769.1"
                     /translation="MKHVLLSLFLGVFMIIASCNNYRNRDQNTADLLQPTPADYLSPR
                     SDTAFFAKLNLDFSGMEKVRKRVKREDFAGAKAAYLEFRRKKSLAKWDVNPAEKPDSA
                     AGDNYPAADDWIMKHKIRPSMSAPEAILGDDINWEYNPVAPTEPHFSKEWTWQNLNRM
                     PNWNILAAAYWYTLDEKYAEEWVAQVIDWVKDNPVPLEEGPGSTLTWRTIEAGIRMAG
                     SWMNSYYHFLNSPSFTPEAHTAFVIGVIEHGQRLEKTAADFPERSGNWVTMECNGLGT
                     IGILFPELKMADRYRAIAFDRLLNELDKQVYPDGAQIELAPGYHQVSRSNFMELAKTA
                     QLNNVNIPEGYMDRLKKMYEFNLYLMDPSGTLPPFNDSGPTRTASSLQEAYEIWKDQR
                     FLFGATLGREGEKPGFDSYFFNYAGYYVMRSGWERKDNCLFFDAGPVGYGHEHEDMLN
                     LYLYSRGKILLTEPGSYSYDLSEWRRYALSTPSHNTILVDGKEQHRADIRKSRLITEL
                     LKNPWITTKLFDYGAGTYSSGYQENRYKPVQYMPKEYVGPKDTSVFHNRHVIFLRPWY
                     FIEVDFLEGTGVHTYESHFHLDAPDAVMAKGAMSVQTLRQDSVQLGLWPMDTERLQAK
                     IVKGQVNPLLGWMPREKRPIPTVVYLKKEEAPAVFSTLIYPYFISVPEVSYIGITGNS
                     SLWGKKITTPYENAVVVINKAVPNAVWDIVPVIAPEFKTDAKVSIIRVLKGKNQICYG
                     FYRLKEFNDKTLAFSVDTEISILIVKDTEGRQVLFNPGGTEIKLSFSLPLKQVVTLTP
                     FKWTEVSSTGISERNEDVLNDLKL"
     gene            3714..6665
                     /locus_tag="PK288_07315"
     CDS             3714..6665
                     /locus_tag="PK288_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPM18770.1"
                     /translation="MSGRIIALILAFSLTAGSLSQSYSSGPSKNRPAFPWPAGKKLAI
                     SLTFDDARLSQADKGIPLLDKYGVKATFYISPNSTMQRLEAWKKALQTGHEIGNHSLV
                     HPCMGNFEFARDKALEDYDLSYMKSELDSAGRFISETFGVTALSFAYPCGQTFIGSGV
                     NTKSYVPLIAAMFESGRGWLGESPNDPWYCNMSQLTGIELDGKSFEQILDLINAAASK
                     GQWLLLAGHEMSDGGRQTSLLSTIDQLCKYASDPAIGIWIDNVHNIAAWIREQRGEGP
                     FTRLPVYKDALFSTKERVDDLLSRMTIEEKIGQMNMPCVYVDELGKDVPAKTDACLKL
                     AEGKFPALPGPAGGFFTLANTILHEGTLQQANFFNQLQKTAVEKTRLGIPLLQTKEGT
                     HGLMCTGGTIFPEGLALGSTWNMDLVSKIYSIAAREARAVGIHQIFTLVIEPNRNPRL
                     GRNQEGYSEDPYFCSRMAETIVNAVQGNDITAPDKTIAGLCHYPGQSQPISGLDRGSM
                     EISERKLREVFLPPWFAGINTVKYMGLASTDGEAGMLNLQAGLDVGISFEDGFIMPMI
                     ENVKEGKVSMHLIDRAVRRILELKFRLGLFENPYVNPDRAVKVTHTEESRGVALEAAR
                     EGIVLLRNENNTVPLKKNLRRIAVIGPNADNEKNQFGDYTANVVNQDIVTVLEGIRSS
                     VAPGTIVDYVKGCNVTGTELNEISKAVQTARKADVAIVVLGENEWQSPKKTGTDGEGY
                     DVATLELTGMQENLLKAIKATGKPVVLVLINGRPLAVMWAAENIPAILEAWIPGEEGG
                     TAIADILFGDYNPSRKLTITVPRHAGQRPVYYDYMPSKEYWLENAWGKPYADMLPTPL
                     WEFGFGLSYTKFEYSNLRVEPAETGTFGEIMISADVKNTGPKEGKEVVQLYIRDLKAS
                     VEVPVKELKGFEKVSLAPGETKSVKFIVTHEDLMFLDRSLTWVVESGDFQVLVGSSSA
                     DIRLKGNFILK"
     gene            6694..8592
                     /locus_tag="PK288_07320"
     CDS             6694..8592
                     /locus_tag="PK288_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPM18771.1"
                     /translation="MKVNNICLTTIAVILFAGSPCILPASEVNSRADQDTQPKKLENP
                     VTVQYLKSTLRKNTPRLVLTPAIEKNLRSKLKTDPVVKNYYANMKVSAKDILTRPALT
                     RNVIGRRLLGTSREMLSRMTILSMVYRIDREPAVLKKINEELLAVCNFTDWNPYHFLD
                     VAEMSMAVAFAVDWVGNWLPKSTLELAKTSLIRKGLEPSFVKGNFGWVNGGNNWNQVC
                     NGGMIAAAIAIAEKDPELAAKTISRSLDGMPGALRQYAPSGVYPEGATYWDYGTSFSV
                     TTSSILQSAFGTDFGLAAYPAFLESANFKMLSEAPSGYYFNFADCGDRAGSNGDIILA
                     WFAMKTGNPLYLEKENFLRPVEGTPRLSRLAGPGLVWLSQFTEKTGSTLPLNWFGDGS
                     NPLVVFRGGEKDPKNYYFGGKGGQGTLSHGNLDAGSFVFELNGVRWAIDPGNQDYNTL
                     EQAGFNLWSSCQECERWTLLTKGNQGHSTLTVDDARFPVSARAPITDFKAGPVPEATV
                     DMTPIFRGRLQSAKRKFIKDSDHSIVIEDQIVLEDSTKSITWAVMTTAEVTPTPDGAV
                     LQQDGKELLLKISSPAGVRISTLMMDPPPLKLDKRIPNLKRIEIRMPAYIFTEGKGTV
                     RVRLTAPE"
     gene            8793..10748
                     /locus_tag="PK288_07325"
     CDS             8793..10748
                     /locus_tag="PK288_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPM18772.1"
                     /translation="MKNSKLLIATLLLSIFSFSVLNAELKPRERNILANEAKAINLAS
                     AMITDNSWNKLPGYKSRSFWDGLPAGTRQEYIGRAEKYLTYDWPVVKATDYLEFIRSG
                     DRRQQVYSACSNALISLVMGELVEGKSRFLDQIINGVWYYSEQTWWGWSAHLGPQKAG
                     SGLPDVNDPIVDLGVGEVTSNLSWTYYLFKEEFDKVHPLISKRLLQAITTKALTPYFE
                     REDFGYMGFRGGRPNNWNPWVNYNMLTSYLLLETDPVKKVAEVTKILNSLDKFINGYS
                     DDGGCDEGPSYWGAAGALMYENLEIMKNITNGKFDVFDEPLIRNMGQYFYKVNIHAPY
                     FINFADADATTGGNASIVYRYGKAINDPIMWKFGAYIAQKGYKGEGALSGKIGDQIKN
                     LGIIPEILKAEGEEALVADFWFPDTEVAGARDKAGSFTGFFFGAKGGFNAESHNHNDI
                     GSCLMYFDGKPCLIDLGREEYVAKTFSSRRYEIWTMQSGYHNLPAINGADQRQGADFK
                     ARNSTFAANAKSATFSTDIAGAYTADAKVKSWIRSYTLNRGKSFVIRDNYILSEVVPG
                     KTTSSNLITYCKVTQVKPGLLKFQGDGFTLNMSYNPKVVAPKIEFIEITDRSLKRYWP
                     DGVTRVKLEFLNPGLKGSDTVTFTKSV"
     gene            10760..12664
                     /locus_tag="PK288_07330"
     CDS             10760..12664
                     /locus_tag="PK288_07330"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPM18773.1"
                     /translation="MAKKHLLTSIVSVFLLSGILQVLIAQESRFPQMTLPEKLLASLK
                     SGHPRLHATKADFEALKGRITTDETLKKWYDAVKTSGDRILGEEPSIYIIPDGLRLLS
                     TSRRVVNRMFTLGFLYQMEGDKKYAERAWKELEAVSKFPDWNPKHFLDVGEMTNAFGI
                     AYDWMYDYWNTDQKNIIKSAIIEKGLCRAMMAYEGVAVQNHSWWIRSEHNWNQVCHGG
                     IGIGALAIADEDPKLAEYILRNVIKFLPYAMVHFGPDGAWNEGPGYWGYATQYNIYIL
                     SGLETALGTDFGLSNIEGFSKTVLFPMYLTGPFNRSFNYADGGDGPTGGSQLYWLAAK
                     YNAPLAAKYQLQLTRSGSPLDLLWYSKELADRQAQLPPLAAYYRASEVATMRGAWDDR
                     NTWFVGFKAGDNNANHSNLDLGSFVLDAYGKRFIMDLGADNYNAPGYFSTGANGPRWN
                     YYRMRAEGHNTLVINPGTKADQDPLAFAGIKKFNSKGNTAFAVADLTPAYAAEAKSVQ
                     RGIALAGGNTVVIRDEVTTGKPSQLYWFLHTRAELKLAGNAKRATLTIDSAQVVAEIV
                     SPANAKFTIMNAEPLATSPKAENNKNEGIRKLAISLAGIANTNIVVVVRPVKGRASAA
                     AAFLKPLSSW"
     gene            12752..13180
                     /locus_tag="PK288_07335"
     CDS             12752..13180
                     /locus_tag="PK288_07335"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18774.1"
                     /translation="MVTGILREGGNESRVAMLPGEAAVLQKLGTQVLVENGAGANSFS
                     TDEEYTKAGATPVSRKEIFSKSDLILSVNAVPVDDLGYCRKGQVLCSVINVAESGEWL
                     EKARTAGLTVLALDIIPRTTRAQAMDILSSIPSPSMIVYP"
BASE COUNT         3809 a         2824 c         3247 g         3332 t
ORIGIN      
        1 acctttatgc attatggacc cattccccag aatgagatcc ttaagttcag tgcattgatc
       61 cagaacaagg ggtggtaagt atcagtatgt ataagaacag aaaaactaaa cacaaagaac
      121 tatgaagaaa ttattaatta tactggcact tgttaccggt ctgattgcct gcgaaaactg
      181 ggaacagaaa tttgatgact ataatttcac aggtgtttat ttcccgtatc agtacccggt
      241 aagaactctg gtattaggtg atgatatcta tccgaatgag aatgataaca accatatgtg
      301 tatcatttac gctgcttttg gcggagttta tgagaataag ttcaatcgtg ttgtaaactt
      361 cactcttgat cagtcacttt gcaacagtgt caggtttggc ctttcagcag gcgacactgt
      421 tcgactgatg ccaacaacat attatactct cagctcaaac agctcactta caattcctaa
      481 aggtgagttc atgggaggtg ttgaggtcca tctgacggat gctttcttta atgatccgat
      541 gtcggtgggc ataaattatg ttataccgct cagaataaca ggcacttcgg acgttgactc
      601 tgtactttca ggcagaactt caaaagaatc acccgatcgc agagtagccg gggattggga
      661 tgttctgccc aaggatttca caatgtatgc tgtaaaattt gttaatccat ggcacgggca
      721 ttatctgcac cgaggagtaa cagttacaaa ggagattgct ccggatacag ttgtaaacac
      781 tcaggttcag caccagaaat tcattgaaaa ggacgaagtg tggactgtca gtacttccgg
      841 gaagaataag gttacctact ccggtacact ccgttccaac aagctttcag gcgatctaaa
      901 aatggatctt acctttgatg caaacaataa ttgtgtgatc tcagatgcag gatcggcctt
      961 tcctgtttca ggtacaggta aattcgtgaa aaacgcagaa tcgtggggca acaaggatcg
     1021 taatgtaatt taccttacat ataccgctac tgatggtaca tactccctta ctgcgaatga
     1081 tacacttgta gtccgggata ggggaacagt gttacaaact tacaatcctg tgattttgac
     1141 caagtagttt aaggtttttt ttaacagatc agcatgttga tcttcactct ggaatgagaa
     1201 gccggttaag acaatatgct gattaagtta ttaataaaag atatcataat gaaacatgtt
     1261 ttattatccc ttttcttagg agtattcatg attattgctt cctgtaataa ttaccggaac
     1321 agggatcaga atactgcaga cctgcttcag ccaacgccag cagattattt aagccccagg
     1381 tcggatacag cgttttttgc aaagctgaac ctcgattttt ccggtatgga gaaagtaagg
     1441 aagagagtta agcgggaaga ttttgccggt gcaaaagcag catacctcga attcagaagg
     1501 aagaaaagcc tggctaaatg ggatgtgaat ccggcagaaa aaccagattc ggcagcagga
     1561 gataattacc cggccgctga tgactggata atgaaacaca agatacgtcc gtcaatgagt
     1621 gccccggagg caatccttgg agatgacata aactgggaat ataatcctgt agctccaacc
     1681 gaaccccatt tttcaaaaga atggacctgg cagaacctga atcgtatgcc gaactggaac
     1741 atccttgcgg cagcatactg gtatacactc gatgaaaaat atgcagagga atgggttgct
     1801 caggtcattg actgggttaa ggataatcct gtgcctcttg aagaaggacc gggcagtacc
     1861 ctgacctggc gtactatcga agcaggtata agaatggcag gatcctggat gaattcatac
     1921 taccatttcc ttaattctcc gtcattcaca cctgaggctc atacagcatt tgtcattggt
     1981 gtgattgagc atggacagcg gctcgaaaaa acagctgccg actttcctga gcgcagcggt
     2041 aactgggtta caatggaatg taacggactg gggaccatcg gaatactttt cccggaatta
     2101 aaaatggctg accgctaccg ggccattgca tttgacagat tgttaaatga actggataaa
     2161 caggtttatc ccgacggagc tcagattgaa cttgctccgg gctatcacca ggtatcacgc
     2221 tcaaacttta tggagctggc aaaaactgca cagcttaata atgtaaatat tcctgaagga
     2281 tatatggaca ggctgaagaa gatgtatgag ttcaaccttt atcttatgga tccttcagga
     2341 acactacctc ctttcaatga ttccgggcct acaagaacgg cctcttcact gcaggaggcg
     2401 tatgagatct ggaaagatca gcgtttcctg tttggtgcaa cactgggaag agagggtgaa
     2461 aagccgggat ttgattctta ttttttcaat tatgccggtt attatgtaat gcgaagcgga
     2521 tgggagagga aagacaattg tttgtttttc gatgcaggac ctgtcggata cggtcatgag
     2581 catgaggaca tgctgaacct ctatctttat tcgaggggaa aaatacttct gactgagccc
     2641 ggatcatatt cttatgacct ctcggaatgg cgaaggtatg cactttcaac acccagccat
     2701 aacacaattc tggtggatgg aaaagaacag caccgggctg acatcagaaa aagccgcctg
     2761 attactgaac tattaaaaaa tccctggatc acaacgaaat tgttcgatta cggagccggt
     2821 acttattctt ccggttatca ggaaaacagg tacaaacctg ttcaatacat gccaaaggaa
     2881 tatgtcggcc cgaaagacac ctcggttttt cataaccgtc atgtaatatt tctccggcca
     2941 tggtatttta tcgaggtgga ttttcttgaa ggtaccggag tacatacata tgaatcacat
     3001 tttcatcttg atgcaccgga tgctgttatg gcaaaaggag ctatgtcagt acagaccctg
     3061 cgacaagatt ctgtccagct gggactctgg ccgatggata cggaaagact acaggcaaaa
     3121 atcgtcaaag ggcaggtgaa tcctctcctg ggatggatgc cccgtgaaaa aagaccgata
     3181 cccactgttg tatatctgaa gaaagaggaa gcccctgcag tattttcgac actgatttat
     3241 ccttatttta tttcagtacc tgaagtaagt tatattggca ttacaggtaa ctccagccta
     3301 tggggaaaga agataaccac accgtatgag aatgcagtgg tggtgatcaa caaagctgta
     3361 cctaatgcag tttgggatat cgttcctgta atagctcctg aatttaaaac ggatgcaaaa
     3421 gtcagcataa taagggtact caaagggaaa aaccagatat gctatggttt ttacaggctg
     3481 aaagaattta atgacaaaac tctggctttc agtgttgata cagaaatatc cattcttata
     3541 gttaaagata cagaaggcag gcaggtttta tttaatcctg gcgggactga gattaagctg
     3601 tcgttttcgc taccgctgaa acaggtagtc acactcactc catttaaatg gacagaagtt
     3661 tcatcaaccg gtatttcaga gcgaaatgag gatgtattga atgatttaaa actatgagcg
     3721 gcagaataat tgcattaatt cttgcattca gtctgactgc agggagcttg tcccagtctt
     3781 acagctcagg tccgtctaag aaccggcctg cttttccctg gcctgccggt aaaaaactgg
     3841 caatcagcct gacctttgat gatgcaaggt tatcacaggc cgataaaggt attccgcttc
     3901 tcgataaata cggggtaaaa gcaacatttt atatctctcc caacagcaca atgcaaaggc
     3961 ttgaagcctg gaagaaggct ctccagacag ggcatgagat aggtaatcat tctcttgttc
     4021 atccctgtat gggtaatttt gaattcgcaa gggataaggc tctggaggat tatgatctga
     4081 gttatatgaa atcagagctt gattccgcag gcagattcat aagtgaaact tttggtgtca
     4141 ctgctttatc gtttgcatat ccttgtggac agacctttat cgggagcgga gtaaatacaa
     4201 agagctatgt tcctctgatt gcagctatgt tcgaatccgg tcgcggctgg cttggagaat
     4261 ctccgaatga tccatggtat tgcaatatgt cgcagcttac aggcatcgaa ctcgatggga
     4321 aatcctttga acagattctc gatttgataa acgctgcagc ttcaaaaggc cagtggctgc
     4381 ttcttgcagg ccatgaaatg agtgacggcg gccgtcagac gtcactcctc agcaccatcg
     4441 accagctttg taaatacgca tcagacccgg ccatcggcat ctggatcgac aatgttcata
     4501 atattgctgc ctggatcagg gaacaaagag gagagggtcc attcacgagg ttgcctgtat
     4561 ataaggatgc gctcttctca acgaaagaac gtgtggacga tctgttatcc cgcatgacaa
     4621 ttgaggaaaa gattggccag atgaatatgc cctgtgttta tgtagatgaa ttgggaaagg
     4681 atgtacctgc aaagacagat gcatgcctga agcttgccga gggtaagttt ccggctttgc
     4741 ccggacctgc aggtggattc ttcacactgg caaacacgat tctccatgaa ggtacccttc
     4801 agcaggcgaa ttttttcaac cagctgcaaa agacagctgt cgaaaaaaca agactgggca
     4861 tcccgttgct ccagaccaaa gaaggcactc atggactgat gtgcaccggc gggacaattt
     4921 ttcctgaagg tctggcgctt ggaagtacat ggaacatgga ccttgtaagt aagatttatt
     4981 cgatagcggc acgtgaagcc cgggctgtgg ggatccatca gatatttacc ctggtgattg
     5041 aaccaaaccg caatcccagg ctcggcagga accaggaagg atacagcgag gatccttatt
     5101 tctgctcgcg aatggcagaa accatcgtga acgccgtgca gggcaatgat ataacagcgc
     5161 cggataaaac catagccgga ctgtgtcatt accccggaca gagccaaccc ataagcggtc
     5221 tcgatagagg ttcgatggaa atttcagaaa ggaaactcag ggaagtgttc cttcctccat
     5281 ggtttgccgg gatcaatacc gttaaatata tgggactggc atcgactgat ggggaagccg
     5341 gtatgctgaa cctgcaggca ggacttgatg tcgggatatc atttgaagac ggatttatta
     5401 tgccgatgat tgaaaatgta aaagaaggca aggtttctat gcatctgata gacagggctg
     5461 tacgccggat ccttgaacta aagttcagac tcggcctttt cgagaatccg tatgtcaacc
     5521 ctgacagggc cgtaaaggtc actcataccg aagaaagccg cggagttgcc cttgaagcag
     5581 cccgggaagg gatagtcctc ctgagaaacg agaacaatac cgttccgttg aagaagaatc
     5641 tgaggagaat tgcggtaata ggcccgaatg ctgacaatga gaagaaccag tttggagact
     5701 atacggcaaa tgtggtgaac caggatattg taaccgtcct tgaaggaatc agatcgtcgg
     5761 ttgcccccgg gacaattgtt gattatgtta agggatgcaa cgttacagga actgaactta
     5821 acgaaatatc aaaagcagtt cagactgccc gtaaagctga tgtcgccata gttgtcctgg
     5881 gtgagaatga atggcagtcg ccgaaaaaga ccgggaccga cggtgagggc tatgatgttg
     5941 caaccctgga gcttaccgga atgcaggaaa atcttctcaa agcaattaaa gctaccggta
     6001 aacccgtagt gctcgttctg attaacggac gacccctggc tgtcatgtgg gcagcagaaa
     6061 atatcccggc gatccttgaa gcatggattc cgggtgaaga aggcgggact gcaattgccg
     6121 atatcctgtt cggtgattat aatccgtcac gtaagctgac gattacagtt ccacggcatg
     6181 ccggacaacg ccctgtttac tacgattata tgccatccaa agaatactgg ctcgaaaatg
     6241 cctgggggaa gccgtatgct gatatgcttc cgacacccct ctgggagttc ggatttggtc
     6301 tcagttatac gaagtttgag tattcaaacc tgagagtaga accagctgaa accggtactt
     6361 ttggtgagat tatgataagt gcagatgtaa agaatacagg accgaaagaa ggaaaagaag
     6421 ttgtccagct ttatatccgg gatttgaaag catctgtgga agtgcctgta aaagagctta
     6481 aaggatttga gaaggtcagt cttgccccgg gtgaaacgaa gagtgttaaa ttcattgtca
     6541 ctcatgagga ccttatgttc ctcgacagat cattgacctg ggttgtggaa tccggcgatt
     6601 tccaagtgct tgtcgggagc tcctcagccg atatcaggct gaaggggaat tttattttga
     6661 aataaaaaga tatgttaaac aaaaaataac aacatgaaag tgaataatat ttgtctgaca
     6721 accattgcag tcatactgtt tgcaggatca ccttgcattt taccggcatc tgaagttaat
     6781 agcagggctg atcaggacac tcagccaaag aagcttgaga atccagtaac agttcagtat
     6841 cttaagtcaa cgctgagaaa gaacacaccg cgtctggttc tgacaccggc aatagaaaaa
     6901 aacctcaggt caaaacttaa gaccgatcct gttgtaaaaa attactatgc aaacatgaag
     6961 gtgagcgcaa aagatattct tacacgtccg gcgcttacaa ggaatgtaat tggacgcagg
     7021 ttactcggca cctcacgcga aatgttgtcg cggatgacca tcctgtcgat ggtatacagg
     7081 atcgacagag aacctgcagt tctgaagaaa attaatgaag agctgctcgc ggtatgtaat
     7141 ttcactgact ggaacccgta tcatttcctt gatgtggcgg agatgtcgat ggcggttgca
     7201 tttgcagtgg actgggtcgg aaactggctg ccaaaaagca cactggaact tgccaaaaca
     7261 tcacttatca ggaaaggtct ggagccaagc tttgtgaaag gcaatttcgg atgggtcaac
     7321 ggaggcaata actggaacca ggtttgtaac ggagggatga tcgctgctgc gattgccatt
     7381 gcagaaaagg atcccgaact ggctgcaaag actataagcc gatcccttga cggcatgccg
     7441 ggagccctca ggcaatatgc acccagcggg gtttaccctg aaggcgccac atactgggat
     7501 tatggtacca gcttttcggt taccacgtca tcgatccttc agagtgcatt cggaactgat
     7561 ttcggactgg ctgcctatcc tgcttttctt gaaagcgcca atttcaaaat gctgagcgag
     7621 gcaccttccg gatactactt taattttgcc gactgcggtg acagagccgg ttctaacggc
     7681 gacatcattc tggcctggtt tgcgatgaaa acaggcaatc ctctgtacct ggagaaggaa
     7741 aatttcctcc gccccgtcga aggtacgcca agactttcta ggttggccgg ccccggactg
     7801 gtatggctgt cacaatttac tgaaaaaaca ggatcaactc ttccgcttaa ctggttcgga
     7861 gacggctcaa atccccttgt tgttttccgc ggtggggaaa aggatcctaa gaattattac
     7921 ttcggaggaa aaggcggaca gggtacgcta agtcatggca accttgatgc cggaagtttt
     7981 gttttcgaac tgaacggcgt aagatgggcc attgacccgg gtaaccagga ctacaatacg
     8041 cttgagcagg caggttttaa tctgtggagt tcgtgccagg agtgtgagcg ctggacactg
     8101 ctgacaaaag gtaatcaggg tcatagtaca ctgaccgttg atgatgcaag atttcctgta
     8161 agtgcaaggg ctccgataac tgatttcaag gcgggtcctg ttcctgaagc tacagtcgac
     8221 atgaccccga tcttcagggg ccggcttcag agcgcaaaaa ggaagttcat aaaagattct
     8281 gaccattcaa ttgttattga agatcagata gttctggaag attccacaaa aagcatcaca
     8341 tgggcagtga tgacaacagc agaagtaact ccgacacctg acggagcagt tctccagcag
     8401 gacgggaagg aacttcttct taaaatatcc tcacctgcag gagtcaggat ttcaacctta
     8461 atgatggacc cgcctcctct taagcttgat aaaaggatcc ccaacctgaa gaggattgag
     8521 atcaggatgc ctgcttacat ttttaccgaa ggaaaaggaa ctgtcagggt gagactgact
     8581 gctcctgaat agcagatgga attaaaattt caatattgcc ggaagtcata ttttagtctg
     8641 tttataaata tttatatacg atgtattcct ttgtgttcct tagtgataac cttaacggga
     8701 ctttgttgta agaagattta acttcaaagt tgcacaaagg tttactctaa ggatcacaat
     8761 gtaaatgtgg tttaaacaaa aaagctgtca ttatgaaaaa ttcaaaatta ttaattgcca
     8821 ctctgctcct gtcaattttt tcattttctg tattgaatgc agagctgaaa cccagagaac
     8881 gcaacatcct tgccaatgaa gcaaaagcca ttaacctggc atcagccatg ataacggata
     8941 attcatggaa taagctgccc ggttacaaat ccagatcctt ctgggacgga ttaccggcag
     9001 gtacccgcca ggaatatatc ggaagggctg aaaaatacct gacctacgac tggcctgtcg
     9061 ttaaagctac cgactacctt gagtttatac ggtcgggaga tcgccgccag caggtctatt
     9121 cagcctgcag caatgcactt atttcacttg ttatgggaga gctggtcgag ggtaaatccc
     9181 ggttccttga ccagataatt aatggcgtct ggtattacag cgagcaaacc tggtggggct
     9241 ggtcggctca cctcgggccc cagaaagcag gctccggatt gcccgatgta aatgatccga
     9301 tagttgacct cggagtcgga gaagttacat caaacctttc atggacatat tatctgttca
     9361 aagaagaatt tgacaaggtt catccgttga tttcaaaaag actcctacag gcaatcacaa
     9421 ccaaagcgct gactccgtac tttgaaaggg aggatttcgg atacatgggc ttcaggggtg
     9481 gccgtccgaa taactggaat ccatgggtca attacaacat gcttacaagc tatctgctcc
     9541 tcgagaccga tccagtaaaa aaagtagctg aggttacaaa gatcctcaac tcactcgaca
     9601 agttcattaa cgggtattcc gatgacggag gatgcgatga aggcccttcc tactggggtg
     9661 cagcaggtgc acttatgtat gaaaacctcg agatcatgaa gaacataaca aatggcaaat
     9721 tcgatgtttt cgatgaaccg ctcatccgta acatgggaca atacttctac aaggtaaata
     9781 ttcatgctcc ctatttcata aactttgccg atgccgatgc caccacaggc ggtaatgcat
     9841 ccattgttta taggtacggg aaagcaatca atgatcccat catgtggaaa ttcggcgctt
     9901 atatcgccca gaaaggctat aagggagaag gagcgctcag tggtaaaatt ggtgatcaga
     9961 taaagaacct cggcattatt ccggagatac ttaaagctga aggagaggaa gcgcttgttg
    10021 ccgacttctg gtttccggat acggaggttg ccggtgcacg tgataaggca ggcagcttca
    10081 ccggattttt ctttggcgcc aagggaggtt tcaacgctga aagccataat cacaatgata
    10141 tcggatcatg tctgatgtat ttcgacggca aaccatgcct tatcgacctc ggacgcgaag
    10201 aatacgttgc aaagaccttc agttcaagac gttatgaaat atggacaatg cagtcgggtt
    10261 atcataacct gccggcaata aacggtgcgg atcaaaggca gggtgctgat ttcaaagcca
    10321 ggaattcaac ctttgcggca aatgcgaagt ccgctacatt ttcaactgac attgcaggag
    10381 catacactgc agatgctaag gttaagagct ggatccggag ctacactcta aacaggggta
    10441 aaagctttgt gatccgcgac aactatattc tttctgaagt ggttccgggc aaaacaacaa
    10501 gttcaaatct tattacttac tgcaaggtta cccaggtcaa accggggtta ttgaaatttc
    10561 agggcgacgg atttacactg aatatgtcgt ataatccaaa agtggttgca ccaaaaatcg
    10621 agtttatcga gataactgac cgttctctga aacgctactg gcctgatggt gttacccggg
    10681 tgaaactgga attccttaat ccgggcctga aaggttctga tactgtaaca ttcacaaaat
    10741 cagtttaatt acatttgtta tggcaaaaaa acatcttctt acatcaattg tttcagtatt
    10801 cctgctttca gggattttac aagtccttat tgcgcaggaa tcgaggttcc ctcagatgac
    10861 gcttccggaa aaactccttg catcactgaa atccggacat cccaggctgc atgcaaccaa
    10921 agctgatttt gaggcgctga aaggcaggat cacgactgat gagacactta aaaagtggta
    10981 tgatgctgtt aaaaccagcg gtgacaggat cctcggtgag gaaccaagca tatatataat
    11041 tccggatggt ctgcgtctgc tgagtacgag caggagagta gtaaacagga tgttcacact
    11101 gggtttcctg taccagatgg aaggggataa aaaatatgcc gaaagagcgt ggaaagaact
    11161 tgaagcagtt tcaaaattcc ctgactggaa tccaaaacat ttccttgatg tgggagagat
    11221 gaccaatgcc ttcgggattg cctatgactg gatgtacgat tactggaata ctgaccagaa
    11281 gaatattatc aaaagcgcca tcatcgaaaa gggtctttgc cgggcaatga tggcatatga
    11341 aggtgtagct gtccagaacc acagctggtg gatcaggtca gagcataact ggaaccaggt
    11401 ctgccatggc ggaataggga tcggcgctct ggcaatagcc gacgaggatc cgaaactggc
    11461 agaatatatt ctacggaatg taataaaatt tctgccttat gctatggtgc acttcggtcc
    11521 cgacggagcc tggaatgaag gtcccggtta ctggggatat gcaacacaat ataatattta
    11581 tatcctgtcg gggcttgaga cagcgcttgg caccgatttt ggcctgagca atattgaagg
    11641 tttctccaaa acagttcttt tcccgatgta tctcaccggg cctttcaaca ggtcattcaa
    11701 ttatgccgat ggcggcgacg gacctacagg cggctcgcaa ctatactggc ttgcagcaaa
    11761 atacaatgca ccgcttgccg ctaaatatca gttgcagctg acgaggtcgg gttcaccact
    11821 cgacctatta tggtactcaa aggaactggc tgacaggcag gctcagcttc ctccgctggc
    11881 tgcctattac agggcttcag aagttgccac gatgcgggga gcatgggatg acaggaatac
    11941 atggtttgtc ggttttaaag caggcgacaa taacgccaat catagtaatc tcgacctcgg
    12001 gagcttcgtg ctcgacgctt acggaaaaag gtttataatg gaccttggcg ccgataatta
    12061 taacgccccc gggtatttca gcacaggggc aaacggaccc cgctggaatt attacaggat
    12121 gagggccgaa gggcataaca ccctggtaat taatccgggc acaaaggctg atcaggatcc
    12181 gcttgcattt gccggaatta aaaagtttaa ctcaaaaggt aatacggcat ttgctgtagc
    12241 cgacctgacg ccggcttatg ctgccgaagc aaagagtgtg cagcgcggaa ttgcactggc
    12301 cgggggaaat acggttgtca tcagggatga ggtaacaaca ggtaaaccgt ctcagctgta
    12361 ctggttcctg catacaaggg ctgaacttaa gctggccgga aacgcaaaaa gagcaaccct
    12421 taccatcgat tctgcacagg ttgtggctga gatcgtttca ccggcaaatg cgaagtttac
    12481 aataatgaat gccgaaccac tggccacttc tcccaaagcg gaaaacaata agaatgaagg
    12541 gatcaggaag ctggcaatct ctctggcagg aattgcaaat acgaacattg tggtggtagt
    12601 aagaccggtg aaaggcaggg cttctgctgc agccgcgttc cttaaaccgc taagcagctg
    12661 gtaaatatat tgttcaatcg cttccctttc tatgtttaat tgattttccc cttttgtttg
    12721 ctatattgtg gcccaaatta attcaggtta tatggtcaca ggtatactca gggaaggcgg
    12781 caacgaaagc agagttgcaa tgcttccggg agaagcagct gtattgcaga agcttgggac
    12841 gcaggttctt gttgaaaatg gtgcaggagc gaattctttt tcaaccgatg aggaatacac
    12901 taaggctggt gctacacccg tatcgcgcaa agagatattt tcaaaatctg acctcatcct
    12961 ttcggttaat gcagtgccgg ttgatgatct gggatattgc cggaaaggcc aggtcctttg
    13021 ttcagttatt aatgtagctg aaagcgggga gtggctggag aaggccagaa ccgcaggtct
    13081 tacagttctt gcactggata ttattccccg taccacccgc gcacaggcta tggatatact
    13141 atcatcgata ccgtcgccat cgatgatagt atatccatag cctgtgcgcg ggtggtacgg
    13201 ggaataatat cc
//
LOCUS       DAOOYG010000071        13165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4835_length_13165_cov_6.390847, whole genome shotgun sequence.
ACCESSION   DAOOYG010000071 DAOOYG010000000
VERSION     DAOOYG010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4835_length_13165_cov_6.390847"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            465..1592
                     /locus_tag="PK288_07340"
     CDS             465..1592
                     /locus_tag="PK288_07340"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPM18775.1"
                     /translation="MKKILFCSISFLIAVLVLSGCQDRKDPGKSDPFKGESFRIKKGT
                     NIAHWLSQSSARGEQRERFFTEKDVRYIDSLGFDHIRLPIDEEQMWDESGKRHDDAFA
                     LMKNCITWCHDAGLRVIIDLHILRSHYFNADVKPLWTEPAEQDKFIALWKDLSAFLKE
                     WPTAMVAYEPMNEPVADDPEQWNRLLMRLVDSIRTWEPARVIVLGSNKWQSADTFDQL
                     RIPAGDTNLILSYHFYEPFFLTHYQAGWTQLKNFRARVSYPGQIVSDPVIPDEMKASG
                     ARELERAMKRVYNRDTLEYMMRKPFEAARRTKLQLYCGEFGVYENFFPEAKLAWYRDM
                     VSIFREHGVAYANWNYKSGSFGIVDDQNVPSIEVTNILLGR"
     gene            1823..2956
                     /locus_tag="PK288_07345"
     CDS             1823..2956
                     /locus_tag="PK288_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPM18776.1"
                     /translation="MALCLVSAITLLFFSQVDIKAQYSDDSVFPVKGFCINSPSPSEL
                     DRFVKFISDELVPRGVNTLLLLGDYNYQFKTYPQLSDSGALSRDDIKKIVAVCKKNKF
                     RIIPQINLLGHQSWSNKTGNLLKHFPEFDETPHIKMPEKYVWPNDDFLYCKSYCPLHP
                     DVHKVLFAVIDELCDVYEADAFHAGMDEVFYLGDDKCPRCQGIDKAELFAGEVIRVRN
                     HLATRGRELWIWGDRLLEGKITGLGFWEAAYNYTWRAIDMIPKDVVICDWHYERPDQT
                     AVYFAMKGFRVVTCPWRTPATAVIQTEDMARWRKYATPEMRPRYYGMVQTTWMDTGGF
                     MDGFYGNLSARAVGAAHPKNPDFTPWATFKAMFTKIAELEKMK"
     gene            3045..4760
                     /locus_tag="PK288_07350"
     CDS             3045..4760
                     /locus_tag="PK288_07350"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HPM18777.1"
                     /translation="MLDMRQRPDTVGLFEKFEVSFNINNGNLYVNPFDPEEIDIMAEF
                     VAPSGKVWKIPGFYYYSGGTLWKLRFSPDEAGTWSYTVKIRDMGGEFTSARHTFTAVG
                     SSRKGPVVVADNKRYLKHSNGTDFYGVGLWYNDSYSGFGTGQVRPAELDRLKSLGVNF
                     ISSFITPLETPGSGLGRYDQNICGRLDQLLEMLEERDMILSLNIWFHSFLSETVWGGG
                     NIRWYANPYQQITGAKDFYRSELAWKYQEKLYRYIIARWGYSRALGIWFIIDEVNGTD
                     GWVTGDSLAAAGWGRKVHNYLKANDPYNHPTTGTRSGGIREFWHEGYQIFDIAAREIY
                     EAQGFPINKTSTLDSASVNPLTDSYRNYADQNQKLWHGYGKPVINGETGWDHTFYEPA
                     MPGYLAMYHNALWVTLATGSAMTPFWWAYSRMTNDNVVNQQMTSIRRFTDRIPFSKLT
                     DVSPATVKTLRGDAYGMKSNEMIFGWIVNCDADVAGEKVTVSSIKNGKYILRLYHTWR
                     GRFIEEKEVECIDGTITFSPPYMRITDSHANYIGQDMAFILEPVPEPAAIPVQAKSVR
                     RSRNK"
     gene            4926..7271
                     /locus_tag="PK288_07355"
     CDS             4926..7271
                     /locus_tag="PK288_07355"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPM18778.1"
                     /translation="MMKLSDYTRFYSVPLLLIFLISGLIIPIQHASAQKKKVKNEAPP
                     PSSKLVIADKGASLYRIVLPKSSTVYEKKAAQVLQDYLLEISGTALPIITADKPGSSY
                     EILLGQNERLGEKGIDINFNELEADGFVIRTDSMRLIIAGGNFKGTLYGVYTFLEKFL
                     DCRMYSAKVKVIPKRDVIALGPVDLKEIPVIRFRDVHYRVTYDEAFSEWHKLDHDARG
                     GRPAWGSWVHTFNSLVPPEIYYKDHPEYFALRDGKRLPTQLCLSNPEVLKITIQNLRK
                     QIASNPAAKYWSVSQNDNRQYCMCDKCRAIDEKEGSPSGSIINFVNRVADEFPDKMIS
                     TLAYEYGRKAPLTARPRKNVNIMLCSIEARRDIPITEATDPDSRSLVKDFEDWGRIAS
                     DIIVWDYVIQFTNLMSPFPNFQVLQPNLRFFVKNGVTAMFEQGNGDGGGEFAELRSYL
                     ISKLLWNPNENIDTLMNDFLRGYYGAAANPIRQYIDEMREALLASGKPLRIFGSPNEA
                     ADSYLAPALIQRYKMLFDQAEEAVRNKPDVLERVRAARLPLNFAIMEQAKKNFTGENG
                     VFIRTGEKWVVRPEIRSLVDPFTDLCIRTGVTLIKEWGTTPEAYRSSMYRMFYMGRNE
                     HLAFGKKLTLLSPDTTLISAMNLARLNDGIRGGHDPEYNWVSFPGKDLDVIIDLEKPT
                     MVRHIECAFYQRAYWLSIFPAKVDFLVSGDGKKYDLVAVVENTLPIDQWDSFQRDFIA
                     DFRPREVRYIRVVAHSIGNTPGEHPGAGQPARMHIDEIVVE"
     gene            7471..10683
                     /locus_tag="PK288_07360"
     CDS             7471..10683
                     /locus_tag="PK288_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM18779.1"
                     /translation="MIRKKVYQILMRTLFMLICVAFSHSLLAQQRVVSGSVRDVSGAG
                     LPGVSILVKGTTVGTTTDVQGRYSIAAAGDATLQFSFIGYVTQEVAVGNRSVIDMILE
                     EDIQQLGEVVVTALGIERSQKALQSTITKVPGMSLTQARENNLGSSIQGRVAGVNVSS
                     TGAGPAGSSRVIIRGNKSVGSNNQPLYVIDGIPMDNTQFGRAGVWGGTDQGDGMTSLN
                     PEDIESVTVLKGAAAAALYGSRGGYGVINIVTKRGQARKGVGIEFNSNYVFEHYLNFS
                     DLQREYGPGGLANADPNDPNSPRVYSKPISQEQAYGWGSGSMWGPKLDGTPVVQFDGV
                     LRPYSYAGDNWKRFYQTGHIWTNSLALSGGSEKQTFRFSFSDMDNKAIIPNETFKRRN
                     LSLSTNGKYGERLTFNAKVMYSNEKVNNRQMISDSPGNAPQAIWSLPNTLNVNDLKGD
                     PNKPGAVAPGVTTPDLKSVGEEFQPADNNWLQNPWWVAYQFKNENIRDRFITSGTLRY
                     NILKWWWVQGQIGMDWYTRNNNSLVPQGTGYQRGGFKNETSNFVREINMEWMSGVDKE
                     FGKFRLNAFVGGNQMKRESESINVSGENFNIPFYTSVTNTRSQNIGYGYGKSGVNSLF
                     GSAEVSFNNYLFITATARNDWFSVLNPANNSKLYPSLGGSFVFTDAFKNLPEVLSYGK
                     VRAAWGQVATANVGAYSSRITYGLIGQGHLGLPMAGFSGGDNIPNPNLSPALSTELEF
                     GADLRFFQGRLGLEFTYYNQKTTDDILSASISRSSGFLTTSVNIGELSNKGIELLITA
                     TPVQGPVTWDVSLNLAKNKNKVIRLSEGVTEVNVEEPRTRTVYIKHIVGYPFGMITGY
                     RQKTDDHGNLVYESNGSPVRSDVYEILGNGVADWTGGLNNSISWKRIRLDALIDFKFG
                     GDIYSGTNVRLTQWGLHKQTLVDREGGKVVKGVTQTGTDANGKPIYEDFTLTLDQEQT
                     RRYWSNLGERAQENFIYDASFIKLRQISLEYSLPVKLLTKTPFTNLAVSFVGRNLAIL
                     FKNIENVDPESNYTDSNAQGLDYFGMPTTRTYGFNLRVTF"
     gene            10703..12322
                     /locus_tag="PK288_07365"
     CDS             10703..12322
                     /locus_tag="PK288_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPM18780.1"
                     /translation="MKHILKLIQSALLIFIFVAGCDTDKLHDMNINPNAVNQIDVNYF
                     LTAAELSASNNGLRDDRYTDWRTNIGMCAHAIQQLASTSTGTISVGDKYLENDVEPSN
                     APWDHFFAGDGRVTMEIIKQTGPGGWYEGRKINTRHAARILRVLCFHRLTDWYGSIPY
                     FEANQGIEGIFQPKYDKQKDIYADLFKELDEACAAMSPSHPDEGFANADYYFNGDIAK
                     WKKFGYSLMLRLAMRVSNVDAAMANTWVTKAIQGGVMSSNDDNVIATMALGPSEWTNQ
                     NGISRGFAPGDGGQWQSSFLSKTFVDWLMGPDKNSTADDDPRLMIFTAGIIKWTPNEV
                     VFYETDPLKQRGLPNGLDGAMLNTLMGGPTIPYEHFSAVNPLMFEDDEPYMIMHAAES
                     EFLQAEALERGIGSGISGTAKEHYEKGVKLAMQMYTVYDPSFVVTDAQVANYLATYPY
                     GVTKPALEMIGEQMWASKWMNWWDAWSDYRRTGFPQLVPTNYPGNATNGQVPTKLTIP
                     AYEATGNPNYKTGATQPDRLVGKVWWDGGPE"
     gene            12685..>13165
                     /locus_tag="PK288_07370"
     CDS             12685..>13165
                     /locus_tag="PK288_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18781.1"
                     /translation="MIKALTGSQLALFFGLFLTAGCTTDFEGIDEIDREPVIEPDYSG
                     VTIPPNIAPMNFIIREDGKSFSLSVKSSNGYVKKVRTSKGVVRFPLHKWKKMLKDTGR
                     GEISIEITAVVNATEIRHYRPIKIFIANDPVDPWLCYRLLYPGYEAYKQLEIIQRCTE
                     "
BASE COUNT         3670 a         2939 c         3309 g         3247 t
ORIGIN      
        1 ttgaggaggt tgacgacagg gtgacaaaag tcatgactga agccctggac gatgccagga
       61 gtgatcatga ggaaaaatga gccggacagc ccggcggatc acacgattcg tactgcttcc
      121 ggatcattga tgaggccggc atgtctgcct ggcaagggcc aaataatatt taaagtttgc
      181 cccggatgaa ttgctgtata cggatgattt acctctgacg gatacaggaa aacaggttca
      241 gaaaaaccct gcagaaagcg ggaacctgtt tccaaatggt tgttttattg ccggcccggt
      301 atgtttttca gcaggtggtt aggtatattg caatttaagt cttaaattgg ctgatagaac
      361 ctgttccctg cagagctgca attctgttgg gaaaagaact gaccggacgg atcagccctg
      421 attatactga tccgcgattt aaaaaaacac taaaacctgt tcctatgaaa aaaatcttat
      481 tttgttcaat ctcattcctg atcgctgttc tggttttatc cggatgccag gacaggaagg
      541 accccggtaa atctgatccc ttcaaaggtg aatcattcag gataaaaaaa ggtacaaata
      601 tagctcactg gctgtcgcag agcagtgcca ggggcgaaca gagggaaaga ttcttcaccg
      661 agaaggatgt caggtacatc gattcccttg ggtttgatca tatacggctg cccatcgacg
      721 aggaacagat gtgggatgaa tcgggaaagc ggcatgatga tgcattcgcc ctgatgaaga
      781 attgcatcac gtggtgtcat gatgccggac tgagggttat tattgattta cacatccttc
      841 gctcgcatta tttcaatgcc gatgttaaac ctctctggac agagcctgcc gaacaggata
      901 aattcatagc tctatggaag gatctttccg cttttcttaa ggaatggccc acggcaatgg
      961 tggcgtatga acccatgaat gaacctgttg ccgatgatcc ggagcagtgg aaccggctgc
     1021 ttatgcgcct tgttgattcg atcagaacct gggaaccggc cagggtcata gtcctgggat
     1081 cgaacaaatg gcaatccgca gacacttttg atcagctgcg gataccggcg ggtgacacaa
     1141 acctcatttt gagctaccat ttttatgaac ctttcttcct tactcattac caggccggct
     1201 ggacccagct taaaaacttc cgtgccaggg tcagttatcc ggggcagatt gtaagtgatc
     1261 ccgtaattcc cgatgagatg aaagcatcgg gagccaggga gcttgaaagg gcaatgaaga
     1321 gagtgtacaa ccgcgatact cttgaataca tgatgagaaa accatttgaa gcagccagaa
     1381 ggacaaagct gcaattatac tgcggcgaat tcggggttta cgagaatttt ttcccggaag
     1441 ccaaactggc atggtaccgg gacatggtct ctattttcag agagcatggg gtggcatacg
     1501 caaactggaa ctataagtcg ggatctttcg ggatagtgga tgaccaaaat gtgccttcca
     1561 tcgaagtcac caatattctt ctgggcagat aaatccatgc ggtgaaggaa tgggaggcgt
     1621 tagtacggta aatggtgatg cctttcctga aaccttggtg ctaatttatc agcaccatgc
     1681 aatgtacctg tttcggtaaa tttcaattaa tccattaaat catattgtca tgaaacatgc
     1741 ctgtttaaca aaaacttacg acaatgaagt tctgtcctgc gcatttcagc cggcacaaaa
     1801 aatcacctct ttccggttga agatggcact atgccttgtt tcggcgatta ccctgctgtt
     1861 tttcagtcag gtagatatta aggcccagta ctctgatgat agtgtttttc cggtaaaggg
     1921 tttttgcatc aattctccgt caccttctga actggacaga ttcgttaagt tcattagcga
     1981 tgagctggtt ccgcgcgggg taaacaccct tttgctgctg ggcgattata attaccagtt
     2041 caagacctat ccccagctga gcgattcggg tgccctttca agggatgata taaaaaagat
     2101 cgtggctgta tgtaaaaaga ataagttcag gataattcca cagatcaacc tgcttggcca
     2161 ccaatcgtgg tcgaacaaaa cggggaacct tctcaagcat ttccctgaat ttgatgaaac
     2221 tccccatatc aaaatgccgg agaaatatgt ttggccaaat gacgatttcc tgtattgcaa
     2281 aagctattgt ccgcttcatc cggatgtaca taaagtgctt tttgccgtaa ttgacgagct
     2341 atgcgatgtt tatgaggcag atgcatttca tgcgggcatg gacgaggtct tttacctggg
     2401 tgatgataag tgcccgcgct gccagggaat agacaaagct gaactttttg caggagaggt
     2461 tattcgcgtc aggaaccatc ttgcaaccag gggcagggaa ttgtggatct ggggcgaccg
     2521 gctcctggaa ggaaagataa ccggcctggg attctgggaa gcagcataca attatacatg
     2581 gagggccatc gacatgatac caaaagatgt ggtaatatgc gactggcatt acgaaaggcc
     2641 ggatcagact gccgtctatt ttgcaatgaa gggcttcagg gtggtcacct gtccgtggag
     2701 aaccccggcg acagcagtga tacagactga agatatggcg agatggagaa aatatgccac
     2761 gcctgaaatg agaccaagat attatggaat ggtacagacc acctggatgg atacaggcgg
     2821 cttcatggac ggtttctacg gcaacctgtc agccagggcg gtcggggctg cccatcccaa
     2881 aaatcctgat tttaccccgt gggcaacatt caaggctatg ttcactaaaa tagccgaact
     2941 ggaaaaaatg aaataacccg gcagatatga gaaagaacat cagtttatta ttagtttttg
     3001 tctcaatcct tcatttcaac cttacggccc aaaaaaagcc ggaaatgctt gatatgagac
     3061 aaaggcccga taccgtcgga ctatttgaga aatttgaggt atccttcaat ataaacaatg
     3121 gaaacctgta tgtcaatccg ttcgatcctg aagaaataga catcatggcc gaattcgtgg
     3181 ctccatcggg aaaggtatgg aaaataccgg gattttatta ttattcaggt ggtaccttgt
     3241 ggaagctgag gttttctcct gatgaggccg gaacctggag ttatacagtg aagatccgtg
     3301 acatgggcgg cgagtttacc tcagcccgtc acacgttcac agctgtcggg tcttccagaa
     3361 agggaccggt tgtggttgcc gacaacaaaa gatatctcaa acacagcaac ggcactgatt
     3421 tttatggagt gggcctttgg tacaatgaca gctattcagg attcgggaca ggacaggtca
     3481 ggccggcaga gcttgacagg ctgaaatcgc tgggtgtcaa ttttatcagc tcattcatca
     3541 caccccttga aactccggga agcgggcttg gccgatacga ccagaatatc tgcgggagac
     3601 tcgaccagct tctggaaatg cttgaggaga gggatatgat actcagtctc aatatatggt
     3661 tccattcctt cctgtctgaa acggtatggg gcggcggaaa catcaggtgg tacgccaatc
     3721 cctatcagca gataaccggt gcaaaggact tctacaggag cgaactggcc tggaaatacc
     3781 aggaaaaact ataccgctat ataatagcac gctggggata tagcagggca cttggcattt
     3841 ggttcatcat tgatgaggtt aacggtactg acggctgggt gaccggcgac agcctggcag
     3901 cagccggatg gggaaggaaa gtgcacaatt atctgaaagc aaatgatccg tataaccatc
     3961 caactaccgg gacccgcagc ggggggataa gggaattctg gcatgaaggc tatcagatat
     4021 tcgatattgc cgccagggag atatatgaag cccagggctt tcctatcaac aaaaccagta
     4081 cgctcgattc ggcttctgtt aatccattaa cagacagcta ccggaattat gcggatcaga
     4141 accagaagct gtggcacggt tatggtaaac cggtcattaa cggggaaacc ggatgggatc
     4201 atacatttta tgaacccgca atgcccggtt atctggccat gtaccacaat gcactgtggg
     4261 tcacccttgc aacgggctcg gccatgacgc cgttctggtg ggcatattcg cgaatgacca
     4321 atgataacgt tgttaaccag caaatgacaa gcatccgcag atttactgac cggatccctt
     4381 tttcaaagct cactgacgta tctcctgcca cggttaaaac actcagggga gacgcatacg
     4441 gtatgaaaag caatgagatg atcttcggat ggatagtcaa ctgcgatgct gatgtcgccg
     4501 gggaaaaggt gacagtatca tcaattaaaa acggaaaata catcctcagg ctgtatcaca
     4561 cctggagggg acgattcatt gaggaaaaag aggtggaatg tatcgacggc acgataacat
     4621 tcagccctcc ctatatgaga ataacggatt cccatgcaaa ttacatcggt caggatatgg
     4681 catttatcct ggagcctgtc cctgaaccgg cggcaattcc ggtacaagct aaatccgtaa
     4741 ggaggagcag gaataaatga agtttaacca tgtattttaa ccacggagta acacagagtt
     4801 tgacacggag tttgaatgca ataacttgat tgttataacc ccgtgaaact ccatactgct
     4861 ctgtgcaact ccgtggttaa aaatacctta tacgttaacc ataaaatcac aatatttatt
     4921 atccaatgat gaaactatcc gattatacca gattttattc tgttccacta ttacttatct
     4981 ttctcatttc gggattgatc attcccattc agcatgcctc agcgcaaaag aagaaagtaa
     5041 aaaatgaagc tcctcctcct tcgtcaaagc tggtaatagc cgacaagggc gccagcctct
     5101 acaggatcgt actgcctaag tcatcaacgg tttatgagaa aaaggccgca caggtgctgc
     5161 aggactacct gcttgaaata tcaggaaccg ccctcccgat tattactgcc gataaaccgg
     5221 gaagttcata tgaaatattg cttggtcaga acgaacgtct tggagaaaaa ggcattgaca
     5281 tcaatttcaa tgagcttgaa gccgacggtt ttgtgatcag gaccgacagt atgcggctga
     5341 taattgcagg aggaaatttc aagggaaccc tttacggggt ttatactttc ctggaaaaat
     5401 tccttgactg caggatgtac agtgcaaagg taaaggtgat cccaaaacgg gatgtaattg
     5461 ccctggggcc cgttgatctg aaggagattc ccgtgatccg gttcagggat gtgcattaca
     5521 gggtgactta tgatgaggcg ttctccgaat ggcacaagct cgatcatgat gcccggggcg
     5581 gcagacctgc atggggatcg tgggttcata ccttcaacag tcttgttccc cctgaaattt
     5641 attataaaga tcatcctgaa tattttgccc tcagggacgg gaaaagactg ccgacgcagc
     5701 tttgcctgtc gaatccggag gttctgaaaa taaccattca gaacctccgt aaacagattg
     5761 cttcaaatcc ggcggcaaaa tactggtcgg tgagccagaa tgacaacagg cagtactgca
     5821 tgtgcgataa atgccgcgct attgatgaga aggaaggatc gccttcaggt tcaataatca
     5881 actttgtcaa cagggtggct gacgaatttc ctgataaaat gatttcaacc ctggcctatg
     5941 aatatggcag gaaagccccg ctgacagcaa ggccgcggaa aaatgtcaat attatgcttt
     6001 gcagcattga ggcacgacgc gatattccca ttaccgaagc caccgatccc gacagcagga
     6061 gtctggtaaa ggattttgaa gactggggaa gaattgccag cgatatcatt gtgtgggatt
     6121 atgtgattca gttcaccaac cttatgagtc cgttcccgaa tttccaggtt ctgcagccaa
     6181 atctcaggtt ttttgtaaag aacggcgtca ctgcaatgtt cgagcagggg aacggggacg
     6241 gcggcggaga atttgccgaa ctcaggagtt atctgatatc caagctgttg tggaatccca
     6301 atgaaaacat tgatactctt atgaatgatt ttctcagggg atattatggg gctgcggcaa
     6361 atccgatacg gcagtatatt gatgagatgc gtgaagcatt gctggcctcc ggtaagccgc
     6421 tgcgcatctt cggttcaccc aatgaggctg ctgacagtta cctggctccg gcacttattc
     6481 aacgatacaa gatgcttttc gatcaggctg aggaggctgt cagaaataag cctgatgtcc
     6541 tggaaagggt tcgggcggca agactgccac tgaattttgc cattatggaa caggcaaaaa
     6601 agaatttcac cggagaaaac ggagtattca tacggacagg tgagaaatgg gttgtcagac
     6661 cggaaatacg gtcattggtt gatcccttta ccgatctctg cataagaaca ggagtcaccc
     6721 tgatcaagga atggggcact actcccgagg cttaccgttc ttcaatgtac aggatgttct
     6781 atatgggaag gaatgaacat ctcgcgtttg gtaaaaagct tacccttctc agccctgaca
     6841 caaccctgat atcagccatg aacctggcaa ggctcaatga cgggatacgc ggggggcatg
     6901 acccggagta taattgggtg tctttcccgg gaaaagacct ggatgtaatt attgatcttg
     6961 agaagccgac aatggtgcgg cacattgaat gtgcttttta ccagagggca tactggctca
     7021 gcatatttcc ggcaaaagtc gatttcctgg tttcaggtga cggaaagaag tatgatctgg
     7081 tggctgtagt agagaataca ttgcctattg atcagtggga ttcgttccag agggatttca
     7141 ttgccgattt caggccgcgg gaagtccggt atataagagt tgtggcacac agtatcggga
     7201 acactcccgg cgaacatccc ggcgcgggtc agcctgcccg aatgcatatt gacgagattg
     7261 ttgtcgagta acaagtaacg atctggttac taaatggaaa caagacatta atgtcccggg
     7321 atgcctttag tgaaatgtta attttgttaa catgttttta atatatttta agaataatta
     7381 ggatttttaa aaactagttt ctatttttga tttaaacatg catccgttaa atatcgaaaa
     7441 atatttaacg gtgccctaaa actaaaacct atgatcagaa aaaaagtgta ccagattttg
     7501 atgcggacac tattcatgtt gatctgcgtg gcattctctc attcactgct ggcgcagcag
     7561 cgtgttgtat ccgggtccgt gagggacgta agtggtgcag gcctgccggg tgtgtcaatt
     7621 cttgtaaaag gcacaactgt cgggaccact actgatgtac agggaaggta tagtattgct
     7681 gcggcaggcg atgccaccct gcaattttcc tttatcggtt atgtgacaca ggaggttgct
     7741 gtcggaaacc gctctgtgat tgacatgatc cttgaggaag acatacagca gcttggggaa
     7801 gttgttgtaa cggcgcttgg tattgagaga agccagaaag cgctgcaatc cacaattacc
     7861 aaggttccgg gcatgagcct tacccaggcc cgtgaaaaca accttggttc atcgatccag
     7921 ggacgtgttg ccggtgtgaa cgtttccagt accggtgccg gccccgcagg gtcatcacgt
     7981 gttattatca ggggtaacaa atctgtcggt tcaaacaatc agccgctgta tgttatcgac
     8041 gggattccga tggataatac gcagttcgga cgtgcagggg tctggggagg taccgaccag
     8101 ggagacggta tgaccagcct taaccccgaa gatattgaat cggttacggt cctcaaggga
     8161 gctgctgctg ctgctcttta tggctcaaga gggggctatg gcgttatcaa tatcgtgaca
     8221 aagagggggc aggccaggaa aggtgtcggc attgaattca actccaacta cgtgtttgaa
     8281 cattacctga acttctctga ccttcagcgt gaatacggtc cgggaggact tgcaaacgct
     8341 gatccgaatg atccgaattc accaagggta tattcaaaac ccattagcca ggaacaggca
     8401 tatggctggg gttccggttc aatgtggggc ccgaaactcg acggtacccc tgttgttcag
     8461 ttcgacggtg ttttaaggcc ttattcatat gcgggtgaca actggaagcg tttttaccag
     8521 acaggtcata tctggaccaa cagcctcgcc ctttccggag gaagtgaaaa acagactttc
     8581 aggttctcct tctccgacat ggataataaa gccataattc cgaatgaaac attcaagaga
     8641 aggaaccttt cactttcgac aaatggcaaa tacggtgaga ggctcacttt caacgccaag
     8701 gtcatgtatt cgaatgaaaa ggtcaacaac cggcagatga tatccgacag cccgggaaat
     8761 gctccccagg caatatggtc acttcccaac accttaaacg taaatgacct gaaaggcgat
     8821 cccaataaac caggtgccgt tgcaccggga gtcaccacac ccgacctgaa atcagtgggt
     8881 gaggaattcc agccggcaga caacaactgg ctgcagaatc catggtgggt agcatatcag
     8941 ttcaagaacg aaaacatcag ggaccgtttc atcacctccg gaacattgag gtacaatatt
     9001 ctcaaatggt ggtgggtaca gggacagatc ggcatggact ggtatacccg gaacaacaat
     9061 tcacttgttc cgcagggtac cggatatcag cgcggcggat tcaagaacga gacatccaat
     9121 ttcgtcaggg aaatcaatat ggaatggatg tcaggtgttg acaaggaatt cggaaagttc
     9181 aggctgaatg cctttgtcgg aggaaaccag atgaagcgcg aatctgaaag cattaacgtg
     9241 tcgggtgaaa acttcaatat tccattctat acctcagtca ccaacacccg gtcgcagaac
     9301 atcggatacg ggtacggtaa gtcgggtgtc aactcccttt tcggatcagc tgaggtcagc
     9361 tttaacaact acctgtttat tactgcaacg gccaggaacg actggttctc ggtgctgaat
     9421 cctgccaaca acagtaagct ttacccatcc ctgggaggta gttttgtatt taccgatgct
     9481 ttcaagaatc ttcccgaagt tctgagctat ggtaaggtca gggccgcatg gggacaggtt
     9541 gcaactgcca acgtgggtgc ttattcatcg aggatcactt acggtcttat cggccagggt
     9601 caccttggac tgccgatggc aggattctca ggaggcgaca acattccaaa cccgaacctt
     9661 tctcctgcat tgtcaacaga gctcgagttc ggtgccgacc tgagattctt ccagggaaga
     9721 ctaggactcg aattcacata ttataaccag aagaccactg acgatatcct ttcggcctcg
     9781 atatccaggt cttcaggctt ccttacaacc tccgtcaata tcggcgagct ttcgaacaag
     9841 ggtatagaac tccttatcac tgccactcct gttcagggtc cggtaacatg ggatgtctcc
     9901 cttaaccttg ccaagaacaa gaataaggtt atccggctca gcgaaggcgt gacagaagtc
     9961 aacgttgaag agccccgcac caggacagtt tatatcaagc acatcgtcgg ctatcctttt
    10021 ggaatgataa caggatacag gcagaaaacc gacgatcatg gaaatcttgt ctatgaatca
    10081 aacggatcac ctgtaagatc agatgtatat gaaattctgg ggaacggtgt tgccgactgg
    10141 acaggcggcc ttaataattc cataagctgg aagcggatcc gcctcgatgc ccttatcgac
    10201 ttcaaattcg ggggagacat ttattcaggt accaatgtcc gtctgaccca gtggggtctc
    10261 cataaacaga cccttgttga ccgcgaaggc ggcaaggttg tgaaaggcgt aacccaaacg
    10321 ggaacagatg ccaatggcaa gccgatctac gaagatttca cgcttacact cgatcaggaa
    10381 cagaccaggc ggtactggag taaccttggt gaacgcgcac aggagaactt tatttatgat
    10441 gcttcgttca tcaagctgcg gcagatctca cttgaataca gtctgccggt caaactgctg
    10501 accaagactc cgtttaccaa cctggcagtg tcgtttgtcg ggagaaacct tgcaatactc
    10561 ttcaagaata ttgaaaatgt tgacccggaa agcaactata ctgacagcaa tgcacaggga
    10621 ctggactatt ttggcatgcc aacgacccgc acttatggtt ttaacctgag ggtgactttc
    10681 taacctttta aaactgagag atatgaaaca tatattaaaa ctgatacaga gtgcgttact
    10741 gatattcatt ttcgtggcag gatgcgatac cgataaactg catgatatga atatcaatcc
    10801 caatgcggta aatcagattg atgttaacta cttccttact gctgccgagc tgagtgcatc
    10861 caataacggc ttgcgcgacg accgttacac tgactggaga accaatatag gcatgtgtgc
    10921 gcatgccatc cagcagcttg caagcacaag tacaggtaca atatccgtgg gagataaata
    10981 cctggaaaat gacgttgaac cgtcgaatgc accgtgggac catttctttg caggcgatgg
    11041 cagggttacg atggagataa tcaaacagac cggccccggc ggatggtatg aaggtcgcaa
    11101 gatcaatacc cgtcatgcgg cgagaatcct gagagttctc tgcttccatc gtcttaccga
    11161 ctggtatggc agcattccct attttgaagc caaccagggc attgaaggaa tattccagcc
    11221 taagtacgac aagcagaaag atatctatgc cgatctgttt aaggaacttg atgaagcctg
    11281 tgcagccatg agcccgtctc atcccgatga gggattcgcg aatgccgatt actatttcaa
    11341 tggcgatatc gccaaatgga aaaagttcgg atattcgctg atgctcaggc tcgccatgag
    11401 agtttcaaat gtcgatgctg caatggctaa cacctgggtt accaaggcta tccagggcgg
    11461 agtaatgtcg agcaatgatg acaatgttat tgccacaatg gctctcggac cgagcgaatg
    11521 gaccaaccag aatggaattt cacgcggttt cgcacccggc gacggaggac aatggcaaag
    11581 ctctttcctg agcaaaacat ttgtcgactg gcttatgggt ccggacaaaa attcgactgc
    11641 cgatgatgat cctaggctga tgatattcac tgccgggatt atcaagtgga ctcccaatga
    11701 agtggttttc tatgaaactg atccgctgaa gcagagaggt ttgccaaacg gactggacgg
    11761 tgcaatgctt aatacactga tgggaggacc caccataccc tatgaacatt tctcggcagt
    11821 gaaccccctg atgtttgaag atgatgagcc ttacatgatc atgcatgcag ccgaatccga
    11881 attccttcag gccgaagctc tggaaagagg aatcgggagc ggtatttccg gaacagccaa
    11941 agaacactat gagaaaggtg tgaaactggc aatgcagatg tacacagttt atgatccgtc
    12001 atttgtggtt acggatgccc aggttgcgaa ttatcttgca acctatccgt acggtgtcac
    12061 caaacctgcc ctcgaaatga tcggagagca gatgtgggca tcgaagtgga tgaactggtg
    12121 ggatgcctgg tcagattaca gaagaacagg cttcccccaa ctggttccga caaactatcc
    12181 cggaaacgct accaatggac aggtgccgac caaacttacc atacctgcct atgaagctac
    12241 cggaaatccc aattataaaa ccggtgcaac acagcccgac aggcttgttg gcaaggtttg
    12301 gtgggatggc ggtcctgaat gattggactt gcctgtgaaa caacaactta aaaggggtcg
    12361 tccgacccct ttttttattt tcggctctgc gtcttatcct ggctgccggg caatcaaggc
    12421 ttaattaata tattttctat ggcagcccgt atgtacaaat aacagaatgt ttccggctca
    12481 gggttctgtc aacggttatc accgttatcc ggatgaactt tggttaccag ccgggctgtc
    12541 gagctgcagc ttccttaaaa aactgcctga atgattggcc ggcctggtgg tgaatattat
    12601 caaaacaacc ggtcttttga taattaattc ataattttac cctgactaaa tcatgtaaat
    12661 tttaaaccgg atataattca ccgaatgatc aaagctctga ccggatcgca actggcattg
    12721 ttttttggac tgttcctgac agcgggctgc accactgact ttgagggtat tgatgaaatt
    12781 gatcgcgagc cggtaattga accagattac tcgggtgtga ccatcccccc taatatagca
    12841 ccaatgaatt tcattatcag ggaagatgga aaatccttca gcctctcagt taaatcttca
    12901 aatggttatg taaaaaaagt caggaccagt aaaggtgttg ttcggtttcc tctgcataaa
    12961 tggaaaaaaa tgctgaagga tacaggcagg ggtgaaattt caattgagat cacggcagtt
    13021 gtgaatgcaa ctgagatcag gcattacagg ccgataaaaa ttttcatagc aaatgatccg
    13081 gttgatccat ggctgtgcta caggttgctt tatccgggat atgaggccta taagcagctt
    13141 gagatcatac agagatgcac cgaga
//
LOCUS       DAOOYG010000072        13136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4852_length_13136_cov_6.254109, whole genome shotgun sequence.
ACCESSION   DAOOYG010000072 DAOOYG010000000
VERSION     DAOOYG010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13136
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4852_length_13136_cov_6.254109"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PK288_07375"
     CDS             <1..1029
                     /locus_tag="PK288_07375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM18782.1"
                     /translation="VGLGGVILLLHTSYPGDMQGMYWLVLFFGFLSGCGIATFSVGIA
                     QTSYWFPRNRQGSALGIYAGLGNLAPGIFSIILPLFLTRFGFISAYFAWFLFLLLGTV
                     LYGLIGQNSYYFQYRRAGLSDDEARAKAHEHGQEIFPSGGVKESLLTSAAIPGTWALV
                     ILYFTTFGGFIALTAWFPTYWNEFQGFEPVKAGLLTATFSLLASLIRVNGGKAADRFG
                     GERTILISMTMILVSAALLSFSSGIVFSILIVILLALGMGISNGAIFKLVPTYVPKAV
                     GGASGWIGGLGAFGGFVLPPLMGEIAGKYGTEGYARGFLVFVALSLINLLIILVLIRN
                     RKKPGSGH"
     gene            1247..3889
                     /locus_tag="PK288_07380"
     CDS             1247..3889
                     /locus_tag="PK288_07380"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPM18783.1"
                     /translation="MKPNLHYPVCRFALLILLASVLLSCSGKKETASVILLGKEWQIK
                     SSLKTEKGGEELSAAGVEEPGWYRAEVPSTVFGSLVKNGIYGDVFFGKNLEKADPNDF
                     KSSWWYRKSFRINNDGASNWLLEFDGIIYRANIWLNGSRIASSDTLAGPFRQFSFNVT
                     GLIRKDGENYLAVEVFPPAAGDLTVGFVDWNPEAPDRGMGLWREVRLRSTGVVSLKYP
                     FVLTRLDTATLKHAELTVTCEATNNGSRKTTGYAEVSIGDGIRLKQKVKLEPGEKKLV
                     TFKPDDYPALKLENPRVWWTHDLGSPELYDLEVRFTNSGAISDKAATHFGIRDISWYT
                     YPKYGREHKGYRLNGRKILVKGGGWVDQLFLMPDSLKLERQIAYVKHMNMNAIRPEGF
                     WGNNHDMYDICDREGILIMTGWSCQWEWPEYCGKEECDELYGCIQTPEEIELISRSWR
                     DMIKWLRNHPSIFMWMTGSDMLPLPELEKKYIAILAEDDPSRPMVTSASMRKSTISGW
                     SGMKMEGPYEYVPPVYWYIDTIFGGAYGFNTETGPGAQVPPVESMREMIPAELLWPIN
                     DTWEYHCGRHAFNTLDRYNTAMNERFGKPSSLEEYCTKAQMLNYESVRPMFEAFQVNR
                     YDATGIIQWMLNSSWPKLIWQLYGFDLLPNGAFYGTRKALQPLQAIYDYGKNEIVVTN
                     SSPVRHDNLMLRIKVFNLNMKELLSREISFGLAGDDKKAIFRLPDQIKGLDPTYFLDL
                     RIVGVDGENLAINQYLLTQTKHEFDWGRTYFVHTPTRKHQNLRQINGLPDVKLESKLI
                     FTPEDDKMQIIIEVENHSGNLAHSVELMLLEAGTGHFVAPVYLDDNYFSLLPGERRVI
                     KGYCYLDDLNDTKLKLKVSGLNIK"
     gene            3961..4509
                     /locus_tag="PK288_07385"
     CDS             3961..4509
                     /locus_tag="PK288_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPM18784.1"
                     /translation="MTILITALAVLFGGVFLYFYNRLVKLDNLAREAWSGIDVQLRKR
                     HDLIPLLVNTVQGYARHERQLFDAITSLRTRGMSATGVRESEKAEVELSKALGKLFVV
                     VENYPELKASQNFLELQRDISRIENDLQKARRYYNGTVRNYNIIIEQFPSSLVARITG
                     RRSRDFFDIQNESERNVPGVNL"
     gene            4520..6208
                     /locus_tag="PK288_07390"
     CDS             4520..6208
                     /locus_tag="PK288_07390"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPM18785.1"
                     /translation="MKRVFIFFIFISAGVLIFSQERILSYDIYCVIETDGTLLVREQI
                     KVVAEGNEIKRGIYRSFPTKYTDRLGNRFRVGFEVTEVLKNGVPEPWFTENRSNGIVV
                     YIGDRDVELTPGVYEYSLSFRTTRQIGFFRNYDELYYNAIGGDWVFPIEAATVTVQLP
                     AGADVVQKAAYSGFAGSTGCDCEMTVDGNVVTLTTTRPLQPREQLTFAVAWPKGFVTP
                     PSVMQKMTFFLKDNRHVVFALAGLVITILLYYWKWRIAGKDPAKGTIIPLYDPPEGFS
                     PAATGYLAGMGMKERVITSALVNMALNGYITIYHTKKKYSISLVPEAATKLNPEEKAL
                     ADVLFSREPEIELDNKNHELFEEARNKVNDVLKGKLTPEYFKFNTRLLLSGFIFSVIW
                     TILAFIISPSPLVPIILIVLFLALLLLFFWLIKAPTVRGRALMDEAEGFRMYLSVAEK
                     DRLNLADEPELTPGRFEKYLPFAIALGVENAWGKRFENALKRSLKDATDYSPSWYVGP
                     ASAAKFSPLGFTSSVGHSFSSAISSASMAPGSSSGSGGGGSSGGGGGGGGGGGW"
     gene            complement(6338..8602)
                     /locus_tag="PK288_07395"
     CDS             complement(6338..8602)
                     /locus_tag="PK288_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HPM18786.1"
                     /translation="MKTRTLWVGLIVITLISFSVLLFFGKEIYRLKPPIPEKVVSEGG
                     EVLFTMQQIRDGQNVWQSIGGQQIGSVWGHGAYVAPDWSADWLHREAGFILDEWSSAE
                     FGVPFDRLEKEKQVMLEVRLQKALRANTYNESTGILTISGERARAVKSISSYYAGLFM
                     NNPSLEKERTAYSIPANTIKDNDRMAAMNAFFFWAAWACVTERPGSTHTYTSNWPPDP
                     AIGNIPSAGIVLWTGFSVIMLLVGIGLLAYYNARNRSDVLDSSLLPQNDPLGGLHPTP
                     SMKAVLKYYWVVVGLILLQILTGVITAHYGVEGNQFYGLSLASIMPYSISRTWHIQLA
                     LFWIATAWLAAGLYIAPAVSGHEPRFQKAGVNFLFFALLVIVVGSMAGEWMGVMQKLG
                     LVKNFWFGHQGYEYVDLGRFWQAFLFIGFIIWLFLMFRALWPAITKRSESRELLILFS
                     IATVAIGAFYGAGLMWGRQTHLSIAEYWRWWVVHLWVEGFFEVFATVIAAFLFVRMGL
                     LNIRYATAGVLFATIVYLAGGVLGTFHHLYFAGTPTAVIALGATFSALEVVPLVFMGF
                     EAYHNLQLSKATAWVKAYKWPIYFIISVSFWNLVGAGLFGFLINPPIALYYMQGLNTT
                     PLHGHAALFGVFGMLAIGLMLFVLKGLTIKYEWKTKMLSIAFWTMNGGLMLMLLLSLL
                     PVGVLQTVASIKVGMWYARSIEFMQEDHMQVFRWLRLVGDTVFAVGAVALALFVIGLK
                     TGWSIDTTRKLYND"
     gene            complement(8979..9332)
                     /locus_tag="PK288_07400"
     CDS             complement(8979..9332)
                     /locus_tag="PK288_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HPM18787.1"
                     /translation="MKFRVESQGFKMSPSLRKFAREKLGSLNSYYAEIQEIEVTLVME
                     VKGAREVVNCILNIRMPGRDAYVKSTASIFEDAILKAAESARRKLRKRKTQLENAKRK
                     ATPNRRVSAKKASAK"
     gene            9721..10350
                     /locus_tag="PK288_07405"
     CDS             9721..10350
                     /locus_tag="PK288_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPM18788.1"
                     /translation="MKISDIFSHNRKWIAERLESDPEYFVKLAGQQTPEYLFFGCSDS
                     RVSVESLLGTKPGDVFMHRNIGNVVPSNDLNALSVISYAVNHVRIKHIIVCGHYNCGG
                     VKAAMTARDEGLLNPWLRHIRDVYRLHRKELNGIASVERRYDRLVELNVLEQCINIVK
                     TRDVQKALLEKRISIHGWVFDLRTGRLIDLKINIKKIMDEIMDVYNLFE"
     gene            10475..11014
                     /locus_tag="PK288_07410"
     CDS             10475..11014
                     /locus_tag="PK288_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPM18789.1"
                     /translation="MTIIRMHGSLTAGLMSACLLISLSCHAPGRLNAKEPEVRYYQVI
                     RVIDGDTFRVDDGSEKGMAVRLIGIDAPETRRTRYKEAGFYAEESREYLSKLILGKSV
                     RLEFDVDTFDHYHRVLAYVFLRDGTFLNARLLREGYAMVLTVPPNVRYAEQFVKLARR
                     AREKGRGLWKGTMLSGNEI"
     gene            complement(10997..12118)
                     /locus_tag="PK288_07415"
     CDS             complement(10997..12118)
                     /locus_tag="PK288_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM18790.1"
                     /translation="MITRLLEEKILSKLNSGKAIILLGPRQTGKTTLLEKIAGQTGKY
                     LLIDCDDSMVRSQMENANTEDIRRIIGNHKTVFFDEAQRIKNIGLVLKIITDKFKDVQ
                     LLVSGSSSLDLLSEINEPLTGRKWEYYLYPISWAEFSIYAGQLKSIQQLETRILFGMY
                     PEVINKQGNERETLRQLSGSFLYKDLLSYQGIRKPDLLEKLLRAVALQTGSEVSYSEL
                     ASLLQVDRKTIITYLDLLEKAFVIFRLQAFSGNLRNEITSTRKIYFYDTGIRNALLAN
                     FNPLNIRQDTGALWENFLISERMKHLHYNGIWANCFFWRTKDRQEIDYLEEREGKLYA
                     FEFKWNPVKKHRFPVTFLNAYPGSSAELISQDNFSDFIS"
     gene            complement(12232..12849)
                     /locus_tag="PK288_07420"
     CDS             complement(12232..12849)
                     /locus_tag="PK288_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18791.1"
                     /translation="MPSNLNALIRYKTINSCLYGGRRRWSLEELIDRCSEALTEYRGR
                     KSSVSERTIRDDIRVMRSDILGFNAPIKQKDGLYFYDDPYYSILSISLTDSGLIKKIL
                     ETLIRIRPEVNHPELEILIEKLTGLSGIKPSTEIMEHTLDYRIQEFPAGIRYKIDSSA
                     KTGLPSDPVKEAAKKMVKEPGLTIPGDLALCWGDVLGLIHRPFTR"
BASE COUNT         3380 a         3026 c         3275 g         3455 t
ORIGIN      
        1 gtcggtctcg ggggggtcat ccttcttttg catacatcgt atcccgggga tatgcagggg
       61 atgtactggc tggtgctgtt tttcggtttt ctgagcggct gcggtattgc caccttttcg
      121 gtgggcattg cccagacctc atactggttc cctcggaaca gacagggaag tgccctggga
      181 atctacgccg ggttggggaa cctggctccg ggaatttttt cgataatcct gccgttgttc
      241 cttacacgtt tcggctttat atcagcctat tttgcatggt tccttttcct tctccttgga
      301 acggtacttt acgggctcat aggtcagaat tcatattatt tccagtacag gagggccggt
      361 ctgtcagatg atgaagcccg ggcgaaggcc catgaacacg gccaggagat cttcccttcg
      421 ggcggcgtga aggagagcct ccttacttct gccgcgatac cgggaacctg ggccctggtg
      481 atactttatt ttacaacctt cggaggcttt attgcactga ctgcgtggtt tcccacatac
      541 tggaacgaat tccagggttt tgaacccgta aaggcaggcc ttttaacggc taccttctcc
      601 cttcttgcat cccttatccg tgtcaatgga ggtaaggctg ccgacagatt cggcggggaa
      661 aggaccatat taatctcaat gaccatgatc ctggtctcgg ctgccctttt gtcattctcc
      721 tcagggattg tgttcagtat cctgattgtg atcctgctgg ctctcgggat gggtatttca
      781 aacggcgcca tcttcaagct ggttccgact tatgtgccga aggctgttgg cggagcttcc
      841 ggctggattg gagggctggg agcattcggc ggatttgtcc tgcctcccct gatgggagaa
      901 atagccggga aatacggtac agaaggatac gcaagaggct tcctggtttt tgttgccctt
      961 tccctgatca atctgctgat aatccttgtc ctgataagaa acaggaaaaa acctggaagc
     1021 ggtcattgaa aaataccgcg gatttttaca cacttctttt tatgtcaacg gaaacaaccg
     1081 gccgtgaaag gtaatatgac attttcccat atcagggtat atggtcctga tgattcctgt
     1141 ccttttacat gtcctggcac aaaagaattc ggggaaattg accagagcac cccatttttt
     1201 aataaattta taggattact gtaacataaa aaacaataat gatccgatga aacctaatct
     1261 ccattatccg gtttgccggt ttgcattgct gattctcctt gcctcagtgc tgctttcgtg
     1321 ttccgggaaa aaagaaacgg cctctgtaat tttacttggc aaggaatggc agatcaagtc
     1381 atcactgaag acagaaaagg gcggggagga actctccgcg gccggagttg aagaacccgg
     1441 ctggtacagg gccgaagtgc cgtccacagt attcggaagc ctggtcaaaa acgggattta
     1501 tggggatgtt tttttcggga aaaatcttga aaaagccgat cccaatgact ttaaaagttc
     1561 atggtggtac cggaaaagct tcaggataaa caatgacgga gcctcaaact ggctgcttga
     1621 gtttgacgga ataatctaca gggccaatat ctggctcaat ggcagccgga tagcatcgtc
     1681 cgatacactg gcagggcctt tccggcagtt ttcgttcaat gtaaccggcc tgataagaaa
     1741 ggacggggag aattatctgg cagttgaggt ttttcctccg gcagcaggtg atctgacggt
     1801 gggattcgtt gactggaatc ccgaggctcc cgaccgggga atggggttat ggcgggaagt
     1861 gaggctgcgg tcaacaggcg tggtctcact gaaatatcct ttcgtcctca caaggctcga
     1921 tacagccacc ctgaaacatg ccgaacttac cgttacctgc gaagcaacta acaacggaag
     1981 cagaaaaacc acgggttatg ctgaagtttc cataggtgac gggatccgtc tgaagcaaaa
     2041 ggttaaactt gagccgggtg aaaagaaact ggtcaccttc aaacctgatg attatcctgc
     2101 gctgaagctt gaaaacccga gggtgtggtg gacccacgac cttggcagcc ctgaactgta
     2161 tgacctggag gtcaggttca ccaactccgg cgcgatcagc gacaaggcgg caacacattt
     2221 cgggatacgt gatatttcat ggtacacata tccgaagtat ggaagggaac ataagggata
     2281 ccgtcttaac ggcagaaaaa tactggtaaa agggggtggc tgggtcgatc agcttttcct
     2341 catgccggac tcgctgaagc ttgagcgcca gattgcatat gtcaaacaca tgaacatgaa
     2401 tgccatcaga ccggaaggtt tctggggcaa taaccatgat atgtatgaca tctgcgacag
     2461 ggaagggatc cttattatga ccggatggag ctgtcagtgg gaatggccgg aatattgcgg
     2521 caaggaagaa tgcgatgagt tatacggatg tatccagaca ccggaggaaa tagaactgat
     2581 ttccaggtcg tggagagata tgataaagtg gctccgcaac catcccagta ttttcatgtg
     2641 gatgacgggc agcgatatgc ttcccctgcc tgagcttgaa aagaaataca ttgccatact
     2701 tgctgaagat gatccttccc ggccaatggt tacctcagct tccatgagga aaagcacgat
     2761 ttccggatgg tcgggtatga agatggaagg cccgtatgaa tatgtgcctc cggtttactg
     2821 gtatatcgac accattttcg gcggcgcata cggctttaat accgaaaccg gaccgggagc
     2881 acaggtaccg cctgttgaat cgatgcgtga aatgattccg gctgaattgc tctggcccat
     2941 caacgataca tgggaatatc attgcggaag gcatgccttc aatacgcttg accgttacaa
     3001 tacggcaatg aacgagcgtt tcgggaaacc atcctcactg gaggaatatt gcaccaaggc
     3061 ccagatgctg aattatgagt cggtgagacc catgtttgaa gctttccagg tgaaccgtta
     3121 tgatgcaacg ggtatcatcc agtggatgct caattcctca tggcccaagc ttatctggca
     3181 gctctatggc ttcgatcttc ttccaaacgg tgccttctac ggaaccagga aagcccttca
     3241 gccactgcag gcgatatatg attacgggaa aaacgaaatc gtcgtgacga actcctcccc
     3301 ggtcaggcat gataatctga tgcttcgcat taaagtgttt aacctcaata tgaaggaact
     3361 gctgagcagg gagatatcct tcgggcttgc aggagatgat aaaaaagcca ttttccggct
     3421 gcctgatcag attaaggggc tggatccgac atatttcctc gatcttcgga tcgtcggcgt
     3481 tgacggggag aatctggcaa tcaaccagta tctcctcacg cagacaaaac atgagtttga
     3541 ctggggaaga acctattttg tgcacacccc tacaaggaag catcagaacc ttcgccagat
     3601 caatggtttg ccggatgtta aactggaatc aaaattaata tttacacctg aagatgataa
     3661 aatgcaaatc atcattgaag ttgaaaatca ttcaggtaac ctcgcacatt cggttgaact
     3721 aatgctgctt gaagccggaa cggggcattt tgttgcaccg gtttatcttg atgacaacta
     3781 tttctcactg ttgcccggag aaagaagagt cataaaggga tattgttacc tggatgatct
     3841 taatgatacg aaactgaaac tgaaggtaag cgggctgaac ataaaatagc tgctgttctt
     3901 tcggatcggc tgtatccggc taagacacag ggcagagtaa tgaccttaaa aaacaacaca
     3961 atgactatac tgatcacggc attggcagtg ttattcggag gagtattcct gtatttctac
     4021 aacaggcttg taaagcttga taatcttgcc agggaagcat ggagcggcat agatgtgcag
     4081 ctcaggaaac gccacgacct tatacccctt cttgtaaata cggttcaggg ttatgccagg
     4141 catgagcggc agctttttga tgcgataacc tcgctccgta cacgcggtat gtcagccacc
     4201 ggtgtcaggg agtcggaaaa ggcagaggtt gaactttcaa aagccctggg aaaacttttc
     4261 gtcgtggtcg agaattaccc tgagcttaaa gccagtcaga atttcctgga gttgcagcgc
     4321 gatatctcca ggatcgagaa tgatcttcag aaagcccgcc gttattacaa cgggacagtc
     4381 aggaattata atataatcat tgaacaattt ccatcatctc ttgttgccag aataaccggg
     4441 cgcagaagcc gtgatttttt tgatatccag aatgaatcgg aaagaaacgt tccgggggtt
     4501 aatctgtaat ctgttcacca tgaaaagggt attcatattt ttcattttca tttccgccgg
     4561 agtgttaata ttttcacagg agaggatcct gtcatatgac atttattgcg tcattgaaac
     4621 ggacggcacc ctgcttgtcc gggaacagat aaaggtcgtg gcggaaggca atgagattaa
     4681 aagggggatt tacaggagtt ttcctacaaa atataccgac aggctgggga accgtttccg
     4741 ggtgggattt gaggttactg aagtgctgaa gaacggcgtt cccgagccct ggtttaccga
     4801 aaacagatcc aatggcattg tcgtgtatat aggcgacagg gatgtggaac tcactcccgg
     4861 ggtttatgag tattccctgt cattcaggac gaccaggcag ataggctttt tcaggaatta
     4921 tgatgagctg tattacaatg ccatcggcgg cgactgggtg ttccccattg aggctgccac
     4981 ggttacggtt cagcttccgg caggggctga tgttgttcag aaggctgcct attcaggctt
     5041 tgccgggtcc acgggttgcg attgtgaaat gaccgttgac ggcaatgtgg tgacccttac
     5101 caccacacgc cccctgcagc cccgggaaca gctcacgttt gcagtggcct ggccgaaagg
     5161 attcgtcacc ccgccttccg tcatgcaaaa gatgacgttt tttctgaagg ataaccggca
     5221 cgttgttttc gcccttgccg gacttgtcat tacaatattg ctgtattact ggaagtggag
     5281 gattgccggg aaggatcctg ctaaaggaac aataatacct ctttatgacc ctcctgaagg
     5341 tttcagtccc gcggcaacgg gatatcttgc aggaatggga atgaaagaaa gggtaataac
     5401 ctcagcgctg gttaatatgg ctttaaacgg ctatatcacc atttaccata cgaaaaaaaa
     5461 atacagcatc agccttgtac cggaggcagc gaccaagctt aatcccgagg aaaaagccct
     5521 ggccgatgta cttttttcac gtgaaccgga aattgagctt gacaataaga atcacgagct
     5581 ttttgaagag gcacgaaaca aggtaaatga tgttctgaaa gggaaactga cacctgagta
     5641 tttcaaattc aatacacggc tgctgttatc cggatttatt ttttcggtga tctggacgat
     5701 actggcgttt atcatatcac cctccccgct ggtcccgatt attctcattg ttcttttcct
     5761 ggcactgttg ctccttttct tctggctgat aaaggctcct acagttcggg gcagggcatt
     5821 gatggatgaa gccgaaggct tccggatgta tctttcggta gccgagaagg atcgtctgaa
     5881 cctggcagat gagccggagc tgactccggg gcggtttgaa aagtaccttc cctttgccat
     5941 tgccctcggg gtggagaatg cgtggggaaa aaggtttgaa aatgcattga agcggtcgtt
     6001 gaaggatgcc actgattata gtccttcgtg gtatgtgggg ccggcaagtg ccgcgaaatt
     6061 ttcgccgctt ggtttcactt caagtgtagg acatagtttt tcatctgcca tttcctcagc
     6121 ctccatggcc ccgggttcgt cttcgggcag cggaggcgga gggagttcgg gcggcggtgg
     6181 cggtggcggc ggtggcggcg gctggtgagc cggcccgctt ttttccgtat ttaaaaactt
     6241 tatcagcagg gatcctgttt cctgagaggc aaatctgcac agccgtgcaa gcctgtcaga
     6301 aaaacgggaa tccgatcaat acttaatagc agtgcggtta atcgttgtat agcttccgcg
     6361 ttgtatctat tgaccatcct gttttcagtc cgataacgaa cagcgccagt gccacggcac
     6421 ccaccgcgaa aacagtatca cccacgagcc tgagccagcg gaatacctgc atatgatctt
     6481 cctgcatgaa ttctattgat cgtgcatacc acatcccaac ctttatgctg gccactgtct
     6541 gcaatactcc tacagggagc aggctcagca gcagcataag catgagtccg ccgttcatgg
     6601 tccagaatgc aatgctaagc attttggttt tccattcgta cttgatggtc aggcctttga
     6661 gcacaaacag catcaggcct attgccagca tgccgaatac tccgaacagt gcggcgtgac
     6721 cgtgaagcgg ggtggtgttg agtccctgca tgtagtaaag ggcaatgggc ggattgatga
     6781 ggaatccgaa aagtccggct ccgaccaggt tccagaagct gaccgagatg atgaagtaaa
     6841 tgggccactt atatgctttg acccaggctg tagccttgct cagctgcagg ttatgatagg
     6901 cttcgaaccc catgaagacc agcggcacta cctcgagcgc gctgaaggtt gcgccgagtg
     6961 cgataacagc ggtcggggta ccggcaaaat agaggtggtg gaaagtcccg aggactccgc
     7021 cggccaggta aacgattgtg gcgaaaagta caccggcggt tgcatacctg atgttgagca
     7081 gccccatcct gacaaaaagg aaggcggcaa taaccgtggc aaacacttca aagaagccct
     7141 caacccacag gtggaccacc caccagcgcc agtattcggc aatggagagg tgggtctgcc
     7201 ggccccacat gagacctgcc ccgtagaacg cgccaatggc cactgttgca atgctgaaga
     7261 ggatcagcag ctcgcggctc tctgaacgtt tggtgatggc aggccacaat gccctgaaca
     7321 tcaggaaaag ccatatgatg aaaccgataa acagaaatgc ctgccagaat ctgccaagat
     7381 caacatactc gtaaccctgg tggccaaacc agaagttctt taccagtccg agcttctgca
     7441 taacgcccat ccactctcct gccattgagc caacgacaat gaccagcaat gcaaaaaaca
     7501 ggaaattgac gcctgctttc tggaatctgg gttcatggcc tgaaacagcc ggggcaatgt
     7561 acaatcctgc agccagccat gcggttgcta tccagaacag tgcaagctgg atatgccagg
     7621 ttcttgagat tgaatatggc atgatgctgg caaggctcaa tccgtagaat tgattaccct
     7681 ccactccgta atgtgcggtt atcacgcctg tgaggatctg cagcaggata agcccaacca
     7741 ccacccagta gtatttcagt accgctttca ttgaaggagt gggatgcagt ccgcccagcg
     7801 gatcattctg gggaagcagt gagctgtcga ggacgtcgga tctgttcctt gcattataat
     7861 aagcaaggag gcctatccct accagcagca ttatcacgct gaatcctgtc cacaggacaa
     7921 tgcctgccga tgggatgttt cctatggccg gatcaggcgg ccagttgctg gtgtaggtat
     7981 gggttgaccc gggccgttcc gttacacagg cccatgcggc ccaaaagaaa aaggcattca
     8041 tggcagccat cctgtcattg tccttgattg tattggcagg aatggaatat gctgtccttt
     8101 ccttttcaag ggaagggtta ttcatgaaaa gacctgcgta atatgatgaa atgcttttta
     8161 ctgccctggc cctttccccg gatatggtca gtatcccggt gctttcattg tatgtgttgg
     8221 cccgcaaagc tttctgaagc cgaacctcaa gcattacctg tttttccttt tcaagcctgt
     8281 caaaaggaac accgaactcc gcggaagacc attcatcgag gatgaaaccg gcctcgcggt
     8341 gcagccagtc tgccgaccag tcgggagcca catatgcacc atgaccccag accgagccta
     8401 tctgctggcc gccgattgac tgccacacat tctgcccgtc cctgatctgc tgcattgtaa
     8461 aaagtacctc ccctccttca gagacaacct tctcaggtat tggaggtttg agcctgtaga
     8521 tttctttacc gaaaaacaac aatacggaga aactgataag agtgatcact attagaccga
     8581 cccatagagt tcttgtcttc ataaaccgga tgttttggag ttaatgttat cttctgaaaa
     8641 aatatgcgct aaattataca ataacagcaa ttatagcagg aatttatgat aattatctct
     8701 tcggtgtttc catgaatgtt tcaataactt cttttgctgt ctgcattttg ctggcaggga
     8761 ggcataaaat ccggtccgcc aggcctttaa caggaacagg cagctgataa gcgctgaatt
     8821 tccgggatca tcggaccggc tgccggattc tgaaatcatt tcccgggaaa tccggaggaa
     8881 tgacccggag atgccaggca gaaccgctgt ttccaatccc ccgttttcgt gaaaacagcc
     8941 ggtggcttat cggaaaagcc ggtacaggag gagttcagtt actttgctga agcttttttt
     9001 gctgaaaccc ttctgttggg cgtggctttt ctttttgcat tctccagttg tgtcttgcgt
     9061 tttctcagct ttctcctggc cgattccgca gctttcagga ttgcatcctc aaatatggaa
     9121 gcggtggatt taacataagc atccctgccg ggcatcctga tgttcaggat acagttaaca
     9181 acctcccttg cacctttgac ctccattaca agagttactt ctatctcctg tatttcggca
     9241 taatacgaat taagtgatcc cagcttttcc ctggcgaact tccttaagga aggactcatt
     9301 ttaaatccct gtgattcaac tctgaatttc atatatctcc ttttaactgt ttattgttga
     9361 taattccggc agatcatata cctgcatgcc tcagtgcatt gtcagcgaat ttcatttcac
     9421 gataaatata gctgttttct caacacgggg agcagccggg ttgctttaat aattttatct
     9481 tagcgttatt acggattcat ggatttatcg gaagcctgaa ccggcagatt agcggcaggc
     9541 atcattgcaa ccagattaaa actattttaa gatgaaacct acatgattga ccacaatcac
     9601 aaacacgata agaatataat tgtcaatcga gcttgcgagc tcgacaaagt caatcatgtg
     9661 cggttccctg cccgacgggt caggcgggca tcataccatt aaaatcaaaa ttatattatg
     9721 atgaaaatca gtgatatctt cagtcataac aggaaatgga ttgcagaaag gctggagtct
     9781 gacccggagt attttgtgaa gctggcgggg cagcagacgc cggaatacct tttcttcgga
     9841 tgttccgaca gcagggtttc agttgaatca ctgctgggga ccaagccggg agatgtcttc
     9901 atgcaccgga acataggcaa tgttgttccc agcaatgacc tgaatgctct gtcggtgatc
     9961 agctatgcgg tgaaccatgt aaggattaag catattatcg tgtgcggtca ttataactgc
    10021 ggcggtgtaa aggctgcgat gactgcccgg gacgaggggc ttttaaatcc ctggctcagg
    10081 catatccggg atgtataccg cctgcacaga aaagagctta acggcattgc ctccgttgaa
    10141 cggcgctacg acaggcttgt tgagctgaat gtcctggagc agtgcatcaa cattgtgaaa
    10201 acccgggatg ttcagaaggc actgctcgaa aaaaggatat ccattcacgg ctgggtgttt
    10261 gatcttagaa ccggcaggtt gattgatctg aagatcaata ttaaaaagat catggatgag
    10321 atcatggatg tttacaacct ttttgagtag aatttctgcc cctggtcctt tctctgcccg
    10381 ccttttgttt cctttgcatt ccccgggctg ttcccggatt cccggaattg tttaactttc
    10441 cgctgtattg agcgtatgca gcggttttaa ctgaatgact attataagga tgcacggatc
    10501 actgactgca ggactgatgt cagcctgcct gcttatttcc ctttcgtgcc atgctccggg
    10561 caggttaaac gcaaaggaac ctgaagtcag atactaccag gtgatcaggg tcattgatgg
    10621 cgacacattc agggtcgacg atggttctga gaaggggatg gcagtgagac tcatcggtat
    10681 cgatgctccg gagacccgga ggacgaggta taaggaggca gggttttatg ctgaggaatc
    10741 gagggaatat ctttcaaagc tgattcttgg caaaagtgtc aggcttgagt ttgacgtgga
    10801 taccttcgat cattaccacc gggtgctggc ctatgttttc ctcagggacg gcacttttct
    10861 caatgccagg ctgctgaggg agggatatgc catggtattg acggtgccac ccaatgtaag
    10921 atatgctgaa caattcgtca aacttgcccg cagggccagg gaaaagggga ggggattgtg
    10981 gaaaggaacg atgctttcag gaaatgaaat ctgaaaaatt atcctgagag atcaattctg
    11041 cagagcttcc gggatatgca ttcagaaatg taaccgggaa cctgtgcttc tttacgggat
    11101 tccacttaaa ttcaaaggcg taaagttttc cttctctttc ttcaaggtag tcaatttcct
    11161 ggcggtcttt agtgcgccag aagaaacaat tggcccagat tccgttataa tgaaggtgct
    11221 tcatcctttc agatataagg aagttctccc atagggctcc tgtatcctgt ctgatattca
    11281 aaggattgaa atttgccaga agagcatttc ttatacccgt gtcatagaaa tatattttcc
    11341 gggtcgaggt tatctcattc ctcaggtttc cgctgaatgc ctgaagcctg aaaatgacaa
    11401 aggccttttc gagaaggtca agataagtga tgatggtctt tctgtcaact tgcagcagtg
    11461 acgcaagttc gctatatgac acttcgcttc ctgtctgaag cgccaccgct ctgagtagtt
    11521 tttcgagaag atcaggtttc cttattccct gatatgacaa gaggtctttg taaaggaagc
    11581 tccccgacaa ctgcctgagc gtttctcttt cattaccttg tttattaatc acctccggat
    11641 acattccgaa aagaatcctt gtctccagct gctgaatgct tttaagctgc cctgcataaa
    11701 tactgaactc tgcccagctg atgggataca aataatattc ccatttcctt cctgtgagtg
    11761 gttcattgat ttcactcaga aggtcaaggg acgaagatcc gctcaccaga agctgaacat
    11821 ccttaaattt atcagtgata atctttagaa caagtccgat atttttgatt ctttgggctt
    11881 catcaaaaaa tactgtttta tgattgccga taatacgcct gatatcttca gtatttgcat
    11941 tttccatctg agacctgacc atggaatcat cgcagtcaat aagcagatat ttccctgtct
    12001 gacctgctat tttctcaagg agggttgttt ttccggtctg ccttggaccc agtagaatga
    12061 ttgcttttcc tgaattcagt ttcgatagta ttttttcttc aagcagtctt gtaatcatat
    12121 tttggggaat ataatcctca aatataggta tattttagga atataatccc caaaagaaga
    12181 ttttttctcc tttcttcaat taattacggt tgtggtcttt taaaatttca tctatctagt
    12241 aaatgggcgg tggatcagcc ccagaacatc accccagcat aatgctaagt ccccgggaat
    12301 agtcagacca ggttctttta ccatcttttt cgcggcctct ttgacaggat ctgaaggaag
    12361 gcctgttttg gctgaactgt caattttgta gcgaatgccg gctggaaact cctgaatacg
    12421 gtaatccaga gtatgttcca tgatttcagt tgatggttta atgcctgaca gtcccgtcag
    12481 tttttcgata aggatctcga gctcgggatg attgacctca ggacgtatcc tgataagtgt
    12541 ttcaagtatc ttcttaatca gtcccgagtc ggtcaaactg attgagagta tggagtaata
    12601 agggtcatcg taaaagtaca aaccgtcctt ctgctttatg ggtgcgttga atccaagaat
    12661 atcgcttcgc atgacacgga tatcgtccct gatcgttctt tcagatacag aactttttcg
    12721 cccgcggtat tcggtcagag cttcggaaca cctgtcgata agttcttcca gggaccacct
    12781 tctccttccc ccgtaaagac aggaattgat ggttttgtaa cgtatcaggg cattaaggtt
    12841 tgaaggcata taaaaaagat tttttaatgc agaaagactg cagtggcact ggtttcattt
    12901 gtacattaaa gataagaaag tcttacaagt cgtgcaagtg gtgcaagtcg tgaaggttga
    12961 gcagacggtt tcaaaagaag ggacctgcaa gacccgcaag accgcaagac cgcaagaccg
    13021 caagaccgca agacaactat aatgatcttt caactattaa tcaaataatc caggtcataa
    13081 aaacaacaac aaaacaattc acagcttatg gcaacctggc cataatacag gcaagt
//
LOCUS       DAOOYG010000073        13004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_4918_length_13004_cov_5.883698, whole genome shotgun sequence.
ACCESSION   DAOOYG010000073 DAOOYG010000000
VERSION     DAOOYG010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4918_length_13004_cov_5.883698"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PK288_07425"
     CDS             <1..508
                     /locus_tag="PK288_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPM18792.1"
                     /translation="VAAITGSGIFDFLPEKHGIGCAIAGFEPTDILQSVLMLVRQVNL
                     KSPSVEIAYTRVVNAGGNRIALKAMSEVFEPCDTSWRGFGRIAASGLKLREEFAGFDA
                     ERRMPVEIPQGIENPACICPDILRGLMKPCECTLFGKGCTPDNPVGACMVSSEGACNT
                     HYRYNIYE"
     gene            501..1508
                     /gene="hypE"
                     /locus_tag="PK288_07430"
     CDS             501..1508
                     /gene="hypE"
                     /locus_tag="PK288_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HPM18793.1"
                     /translation="MNDTVTLNDGSGGRQSARLIRKVFVRRFGIPLPLTDSAVLEEDG
                     SLLAFTTDSYIVDPVFFPGGNIGKLAVCGTVNDLAVSGAVPMFISASFIIEEGFPLDD
                     LEQIAGSMAEEAENAGVRIVTGDTKVAGRGKCDRIFITTAGIGRLKPQFRSIGTGNKV
                     RAGDRIIVNGSLGNHAVAILGARHDLSFSTQVVSDCASMNHLIQLVLKECSEVHFMRD
                     LTRGGLAAVLNELAQMTGKGMTIDERSVPVDSVVRGLCETMGFDPLHLANEGKIAYVI
                     GESDYMKALEVLRNHPLGSGSAVIGHITDVNPGSVRLITSAGGTRIMEPPSGMLIPRI
                     C"
     gene            1662..2126
                     /locus_tag="PK288_07435"
     CDS             1662..2126
                     /locus_tag="PK288_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPM18794.1"
                     /translation="MYQVGNLVKELTEKHGRARDSLMPVLQGIVAKHSYLTDEAMVEV
                     AKELDISAAEVYGTASFYTFLDTKERGKYVIRVCKTITCAMKGKSEILEVLEDMLKIK
                     VGETTSDKLFTLMETNCIGWCHKAPAMLINEMPFTELTPERVVEIIKSYMKK"
     gene            2136..3302
                     /locus_tag="PK288_07440"
     CDS             2136..3302
                     /locus_tag="PK288_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPM18795.1"
                     /translation="MAMRHKGLNKVDLIFEKVDLREVLTKAFRMSSEEIIQEILESGL
                     KGRGGAGFPTGLKWKYTAAEKDPDKYVVCNADEGEPGTFKDREILDKVAGKMYGGMAI
                     AGHAIGAKKGIVYLRGEYRFLLPQLMRELESFHAMIAEIGYDFHINYALGSGAYICGE
                     ESALFESIEGFRGEPRNKPPYPTTSGVYGKPTSINNVETLVTVLMIIREGAGVYNQLG
                     TKDSRGSKVFSVSGDTPTPGIFELELGMTVQEFVNEFGDGDTKAVQVGGASGFCVPRK
                     RFTDTIIGFEGVPTGGSMKLFNSSRSMYNVLRNYLDFFAEESCGQSTPCRVGCQQLLK
                     GVEKVKKGEVQSSYLNELVKLAETMKIASKCGLGQSVGNAFRTIVENFKEEIIY"
     gene            3327..5108
                     /locus_tag="PK288_07445"
     CDS             3327..5108
                     /locus_tag="PK288_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPM18796.1"
                     /translation="MINLTINDQKIRVPAGTTVLEAAKKLNINIPTLCNHSDLCVAGN
                     CRVCLVELTGARTLIAACAMPVSEGMDIHTNTLKVRNARKHVVELLLSEHRSDCTKCY
                     KNQKCELQALANEFAFGDTIFLDLVEDHPYTIDRSSPSFIKDDSKCIRCQRCVRTCAE
                     LQYISAIAVANKGAHQKISSFHDRPMSHVICTNCGQCVNRCPTGALVEKTYIDHVWDA
                     IYDPDKHVVVQTAPAVRVALGEDLGYDPGERVTGKMVTALKQLGFDSVLDTDFSADLT
                     IMEEGTELLFRLKKALVDGDTSIKFPMFTSCSPGWIKFIEHKYPEFLPNLSTCKSPQQ
                     MFGALAKTFYAQKKKLDPAKMVSVSIMPCTAKKFEADRPEMRASGFKDIDYVLTTREL
                     AIMIKQAGIDFRNLEPGHYDSIMGESTGAGVIFGATGGVMEAALRTAYEIVTGREVPF
                     QNLNIVPVRGMEGVKEASIKIEGCTSEWKFLEGVELKVAIAHGLTNANEIMRLVRQGK
                     APYHFVEIMACPGGCIGGGGQPIPTSMEIRKNRMKAIYSEDEHMVLRKSHENPDVIAI
                     YSEFLGKPNSHKSHELLHTHYVERESY"
     gene            5372..6328
                     /locus_tag="PK288_07450"
     CDS             5372..6328
                     /locus_tag="PK288_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HPM18797.1"
                     /translation="MDPLRISTAQFENRSGDKKYNLSVIGTLAAKAAAAGSDVIAFHE
                     CSVTGYTFARNLDRAQMLDLAEPVPSGDSVSKLVDIAARSGITVLAGLFEKDDAGRIY
                     KAYVCVDGNGLIACHRKIHPFINQYITPGDWYTVFDISGWKCGILICYDNNVVENVRA
                     TALLGAEIIFMPHVTMCTPSPRPGSGFVDPELWKNRAVNPEALRAEFDGPKGREWLMK
                     WLPSRAHDNGIYVVFSNPIGMDDDQLKNGCSMILDPFGNIMAECRTLGDDFVTATVTA
                     DKLTQAGGYRYTRARRPELYRDILGKDHKPVQKVAWLPRQDS"
     gene            6404..7480
                     /gene="dinB"
                     /locus_tag="PK288_07455"
     CDS             6404..7480
                     /gene="dinB"
                     /locus_tag="PK288_07455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPM18798.1"
                     /translation="MRKIIHIDMDAFFASIEQLDNPGLRGKPVAVGGSGERHVVAAAS
                     YEARKFGVRSAMPSLTARRLCPDLIFVPHRFDRYEEISAIIFGIFREYTDRVEPLSID
                     EAFLDVTHDRKGIGSATLIARAVKQEIEKKTGLTASAGISYNKFLAKIASDIRKPNGL
                     FVISPDDAMKFIDELPVEKFFGIGRVTAEKMHRLGIHKGSELKQMDLLSLIRNFGKAG
                     RFYHDIVRGIDNRPVEAFTERKSIGTEVTYEKDLTSQFGIIAELYRVEKELMVRLENA
                     GTTGRTVTVKVKFSDFRQITRSRTLPCYIRDFDTLHREVTAIRKSMDLEGSRIRLLGV
                     TVSNLESDDHADRQLLLFDENVIS"
     gene            complement(7740..8651)
                     /gene="cysK"
                     /locus_tag="PK288_07460"
     CDS             complement(7740..8651)
                     /gene="cysK"
                     /locus_tag="PK288_07460"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPM18799.1"
                     /translation="MKANSILELIGNTPHIRINRLFGEGSEVWLKLERSNPGGSIKDR
                     IALEMIEDAEKNGLLEPGGVIIEPTSGNTGIGLAMVAAVKGYRIILVMPESMSPERRK
                     IMSAYGAQFLLTPREKGMKGAIERSRQLRDEIPGSWIPMQFDNQANVKAHRNTTAMEI
                     LRDFPEGIDYMFAGVGTGGHLTGCASLLKKEFPRLKVYAVEPELSPILSGGTPGPHPI
                     QGIGAGFIPAILDTSLIDEIIKVGKEESYRYARRAARDEGLFVGISTGAVLAAVSQVI
                     DDLKPGSRIMAFCYDTGERYLSVDELF"
     gene            complement(8675..9439)
                     /locus_tag="PK288_07465"
     CDS             complement(8675..9439)
                     /locus_tag="PK288_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HPM18800.1"
                     /translation="MTIPNKKSVEIAVKKLFKTLFPFTGDITAPFTDTGHKTVRDLLV
                     LIFSGLTDDPVDLTDRLMGRMPEIKTILLKDASFMFESDPAARSLEEVIAIYPGFFAI
                     YCYRIAHELERMSVPLLPRMITEYAHKITGIDIHPGAVIGTPFSIDHGTGIVIGETTR
                     IGNHVKVYQGVTLGALSVDKSKASRKRHPTIEDNVILYAGCTILGGNTVVGHHSIIGG
                     NVWLTESVAPFCVVYHKSEIIVKGKNDSPEGYNFII"
     gene            complement(9449..10585)
                     /locus_tag="PK288_07470"
     CDS             complement(9449..10585)
                     /locus_tag="PK288_07470"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HPM18801.1"
                     /translation="MKFETKAIWVGQEADPSTGATILPIYQTSTFSREEADKPGGYEY
                     SRVGNPTRAVLDKCLASLEEGRHALSFSSGMAAEHAVFSLLRPGDHAIVPEDMYGGTY
                     RLIKEILEPAGITFTFADYADYGSLLNSFKPSTRMVWLESPTNPLLKIFDIGKIADTA
                     RDHGALTVVDNTFASPYFQKPLLHGADIVVHSTTKYINGHSDVIGGAVILNDQAVYER
                     IRLVQKSVGAVPSPFDSWLTLRGLKTLAIRLKQHELNAFAVARHLKNIPKVTEVFYPG
                     LEEHPGHAIAKKQMTGFGGVVSFRMEGGLNEANAFFRKLKIFQLADSLGGVESLANYP
                     AIMTHGAFPAGLRKKIGVTDNLIRLSVGIEHIDDLLVDLENAFK"
     gene            complement(10616..11896)
                     /locus_tag="PK288_07475"
     CDS             complement(10616..11896)
                     /locus_tag="PK288_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPM18802.1"
                     /translation="MKLNGENRPIGLLRFTTAGSVDDGKSTLIGRLLYDSKSIFEDQM
                     EHIVQSSKRLGKEETDLSLLTDGLRAEREQGITIDVAYRYFATPARKFIIADTPGHIQ
                     YTRNMVTGASTADLAVILIDSRKGVVEQTMRHTYIASLLDIRHIVFCINKMDLVGYSE
                     DVYLRLCDDLKKLGSRLGTGDLHFIPISAKYGDNVVSPSENMKWYGGKTFLNLIETIE
                     IGKKRNSGPARFPVQTVIRPLSSEYHDYRGYAGRVASGTFSAGDRITVLPSGMESEIS
                     SITVMGKTISKAEAGDSVTLNLTDQIDISRGDMIVKKGEMPESGVDINLMVCWFNEKP
                     LQQGRKYLIRNNTNETTCVVSSINYRMNISNLERETAGNDVNMNDIANISIRTPRPLY
                     YDCYKNNNITGSMIFIEEGTNETVGAGMICGEYD"
     gene            complement(11996..13003)
                     /gene="cysD"
                     /locus_tag="PK288_07480"
     CDS             complement(11996..13003)
                     /gene="cysD"
                     /locus_tag="PK288_07480"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HPM18803.1"
                     /translation="MRAAFKIEYRDQEIQENMDNSVCSKRNDNAMKEQTLAINDNHLK
                     ELEDEAIFVMREVASQFEKKVILFSGGKDSIVLVHLARKAFWPAKMPFSLLHIDTGHN
                     FEETLKFRDDLANELGVELIVRLVQDTIDQGRVTEERGINASRNKAQSVTLLDAIEEL
                     RIDVAVGGGRRDEEKARAKERFFSHRNEFGQWDPKNQRPELWNIFNGHKNFGEHFRVF
                     PISNWTELDVWQYILKEGIRLPMLYYTHRRKIFLRNGVYLAWAPFMQLKPSEKVFEAD
                     IRCRTIGDITCTGVTMSKAGSLEEIISEIAATRVTERGGRYDDMRSEAAMEDRKKEGY
                     F"
BASE COUNT         3327 a         3083 c         3251 g         3343 t
ORIGIN      
        1 tgtggctgca atcaccggtt cagggatctt tgactttctc ccggagaagc acggcatcgg
       61 atgcgccatt gcaggatttg aacctaccga catacttcag tcagttctca tgcttgtccg
      121 ccaggtcaac ctgaaatcgc catcagttga gattgcctat acacgcgtcg ttaatgccgg
      181 cggcaacaga attgccctga aggccatgtc ggaagttttc gaaccatgcg ataccagctg
      241 gaggggtttt ggaaggatag cggcaagcgg actgaaactc agggaagaat tcgccggatt
      301 tgatgcagaa agaagaatgc cggttgagat cccgcaggga attgagaacc ctgcctgtat
      361 ttgccccgat attctcagag gtctgatgaa gccctgtgaa tgcacccttt tcggaaaagg
      421 atgcactcct gacaatccgg ttggagcatg catggtttcg tcagaaggag catgtaacac
      481 ccattacagg tacaatatat atgaatgaca cggttacact caacgacggc agcgggggaa
      541 ggcaatcggc caggcttatc cgaaaggttt ttgtcaggcg gttcgggata ccgctgccgc
      601 ttaccgattc ggcagtgctg gaagaggatg gaagcctgct tgcttttacc accgattcct
      661 atatagttga tccggtgttc tttccgggcg ggaatatcgg caagcttgca gtctgcggaa
      721 ctgtaaacga cctggccgtt tcaggcgccg tgccaatgtt catatctgct tcatttataa
      781 ttgaagaagg ttttccgttg gacgatcttg agcagattgc cgggtcaatg gctgaagagg
      841 ctgaaaatgc aggcgtcagg atagtcaccg gcgataccaa ggttgcaggc aggggcaagt
      901 gcgacaggat ctttattacc actgcaggga ttggcagatt gaaaccgcaa ttcagaagta
      961 taggcacagg aaacaaggtc agggcaggcg acaggatcat tgtgaacgga tcgcttggga
     1021 atcatgcagt tgccatcctt ggagcgcggc atgacctgag tttcagtaca caggtcgttt
     1081 ccgactgtgc atcaatgaac cacctgatac agcttgtgct taaagaatgc agcgaagttc
     1141 atttcatgag ggatctgaca cgcggaggac tggctgctgt tctcaatgaa cttgcccaga
     1201 tgacaggaaa gggaatgacc attgatgaaa gatctgttcc tgttgacagt gttgtcaggg
     1261 gcctttgtga aacaatgggc ttcgatcctc tgcatctggc caatgaagga aaaatagcat
     1321 atgtaatagg cgaaagcgat tatatgaaag cacttgaggt attgcgtaat catcctctcg
     1381 gatcaggctc cgccgtgatc ggtcatataa cggatgttaa tccgggaagc gtcaggctta
     1441 taacatccgc cggaggtaca agaattatgg aaccgccttc aggtatgctc ataccaagga
     1501 tatgctgaaa acccggatct accttattta atttcattct aaataatctt ttatgaccaa
     1561 tataattttt ttaaaaaagt attgttatta ttttaacagc acctgaaaaa acacattttt
     1621 aaccagggat attttattaa ttatctaaaa atcaatatga aatgtatcaa gtaggaaatc
     1681 ttgtaaagga actgactgaa aagcacggcc gggcaaggga cagtctgatg cccgtacttc
     1741 agggtatagt ggctaaacac agttacctta ccgatgaggc aatggtggag gtggcaaagg
     1801 aactggacat ctcggctgcc gaggtgtatg ggacggcctc tttttatact ttcctcgaca
     1861 caaaggagcg tggcaaatat gtgataagag tctgcaaaac aattacatgt gcaatgaagg
     1921 gtaagagtga gatccttgag gttctggaag acatgcttaa gataaaagtc ggggaaacca
     1981 cttccgacaa gcttttcacc ctgatggaaa cgaattgcat cggttggtgt cacaaagccc
     2041 cggcaatgct gatcaatgaa atgcctttca ctgagcttac tccggaaagg gttgtggaga
     2101 taataaaaag ttacatgaag aaataaataa ccgttatggc aatgagacac aaaggattaa
     2161 acaaggttga tctcatcttt gagaaggtag atctgaggga ggttctgacg aaagctttca
     2221 ggatgagcag tgaagaaata atccaggaga ttcttgaatc aggactcaag ggccgtggcg
     2281 gtgccgggtt ccctaccggg ttgaaatgga aatatactgc tgctgagaaa gatcccgata
     2341 aatatgtcgt ttgcaatgct gatgaaggcg aaccgggaac tttcaaggac cgggaaatac
     2401 ttgacaaggt cgccggtaag atgtatggcg gaatggctat tgcgggccat gcgataggag
     2461 caaaaaaagg aattgtttac ctgcggggcg aatacaggtt ccttcttccg cagctgatga
     2521 gggagcttga atcgttccac gccatgatag ccgaaatagg ttacgacttc catatcaatt
     2581 atgcccttgg aagcggtgca tatatatgcg gtgaagagag tgccctattc gaatcgatcg
     2641 agggattcag gggagaaccc cggaacaagc cgccttatcc caccacttca ggtgtttacg
     2701 gtaagccgac atccattaac aacgttgaaa ctctggtgac tgtgctgatg attatcaggg
     2761 aaggcgcggg ggtttacaat cagcttggaa cgaaggattc caggggatcg aaggtatttt
     2821 ccgtatcggg cgacactccg accccgggaa tatttgaact cgagcttgga atgaccgtgc
     2881 aggaattcgt gaatgagttc ggcgacggtg acacgaaggc tgtacaggtg ggcggcgcct
     2941 caggtttctg cgtgccgagg aaaaggttca ccgacacgat aattggtttt gagggggttc
     3001 ccaccggcgg ttccatgaaa ctgttcaaca gttccaggtc aatgtacaac gtattgcgca
     3061 actatctcga tttttttgcc gaagagtcat gcggacagag tacaccctgc cgcgtaggct
     3121 gccagcagct tctcaagggt gttgagaagg tcaagaaggg agaggtgcaa tcatcatatc
     3181 tcaatgagct tgtcaaactg gccgaaacca tgaagattgc ttccaaatgc gggcttggcc
     3241 agtccgtcgg aaatgccttc aggaccatcg ttgagaactt caaggaagag atcatttact
     3301 agtaataaat tgaaaatctg aaatatatga ttaatctgac aataaacgac cagaagatcc
     3361 gcgtacctgc gggtacaacc gttctggaag ccgcgaaaaa actgaatatt aatatcccga
     3421 ccctgtgcaa ccattccgac ctgtgtgtgg ccggaaactg ccgtgtctgc cttgttgaac
     3481 tgacgggcgc acggacactt attgctgcat gcgccatgcc tgtatcggag ggtatggata
     3541 ttcataccaa tacgctgaaa gtaaggaacg caaggaagca tgttgtggaa ctgctgctgt
     3601 cggaacacag gtccgactgc accaaatgct acaaaaacca gaagtgtgag ctgcaggcac
     3661 tggccaatga gtttgctttc ggtgacacaa ttttccttga cctcgtggaa gaccatccct
     3721 acacgataga cagatcatct ccgtcattca tcaaggatga cagcaagtgc atcaggtgcc
     3781 agaggtgtgt caggacatgt gctgagcttc agtatatttc tgccattgca gtggcaaata
     3841 agggtgctca tcagaaaatc tcgtcattcc atgacaggcc gatgagccat gtcatttgca
     3901 caaactgcgg gcagtgcgta aatcgctgcc cgaccggggc cctggttgaa aaaacatata
     3961 ttgaccatgt gtgggatgca atatatgatc ctgacaagca tgtggtggtt cagacagccc
     4021 ctgcggtaag ggttgccctc ggagaagatc tcggttatga tccgggcgaa agggtaacgg
     4081 gtaagatggt gacagccctt aaacagctgg ggtttgattc cgtgcttgat accgatttca
     4141 gcgccgacct tacgataatg gaagaaggaa cggaactgct gttccgcctg aaaaaagcac
     4201 ttgttgacgg ggatacaagc attaaatttc cgatgtttac gtcctgctca ccaggatgga
     4261 taaaattcat tgaacacaag tatcccgagt ttcttccgaa cctgtcaacc tgcaagtcgc
     4321 cccagcaaat gttcggagcc ctggccaaaa ctttctatgc acagaagaaa aagcttgatc
     4381 ccgcaaaaat ggtatcggtt tccatcatgc cgtgcacggc taagaagttt gaggctgatc
     4441 gtcctgaaat gagggccagc ggctttaaag acatcgatta cgtgttaacc acgagagaac
     4501 tggcaataat gatcaaacag gccggaattg atttcaggaa tctcgaaccc gggcattacg
     4561 attcaattat gggtgaatcg accggggccg gcgttatttt cggcgcaacc ggcggcgtaa
     4621 tggaggcagc cctgagaaca gcctatgaga ttgttaccgg cagggaagtg cctttccaaa
     4681 atcttaatat cgtccccgtt cgcggaatgg agggtgtgaa agaagcttca ataaagatcg
     4741 agggatgtac cagtgaatgg aaattcctgg agggtgttga actgaaggtg gccattgccc
     4801 atggtttgac aaatgccaat gagatcatgc ggcttgtacg ccagggaaaa gcaccctacc
     4861 attttgtgga gatcatggcc tgcccgggtg gatgcatcgg aggcggcgga cagcccattc
     4921 cgacaagcat ggagataagg aaaaaccgta tgaaagccat ctattcggaa gatgaacaca
     4981 tggttctgag aaagtcacat gaaaatcccg acgtgatcgc aatctatagc gagttcctcg
     5041 gcaaaccgaa cagccataaa tcccatgagc ttcttcatac acattatgtg gaaagggaga
     5101 gttattaaga ttctatgaga gtatcggcga ttgtgccgaa aggtttatgc cctgccggat
     5161 catgatccgg cagggcaggg gctcacccgg aaataaatga acctgatccg gcatggttaa
     5221 ccagcggatg cttccgcctg tgcatcggat cctttaccgg acatccgggc aacagccaga
     5281 cagtacttcc ggcccggcat acgtattttg cagggcttcc cggtgcctta cgactgctaa
     5341 tgttgaaatt gcctgaataa caccgaatca gatggaccca ttacgaatat ccacagccca
     5401 gtttgagaac agaagcggcg acaagaaata caatctttcc gtgatcggaa cactggctgc
     5461 aaaagcagcg gcagccggat ccgatgtcat tgcatttcat gaatgctccg tcacgggata
     5521 caccttcgca aggaatcttg accgggcaca gatgcttgat ctggccgaac cggttccttc
     5581 gggggatagt gtgtcgaaac ttgtcgatat tgctgcaaga tcagggataa cagtgcttgc
     5641 cggattgttt gaaaaagatg acgccggcag gatctacaag gcctatgtgt gtgttgacgg
     5701 aaatggcctt attgcctgtc ataggaagat tcatcctttt atcaatcaat acattacccc
     5761 gggcgactgg tatactgttt tcgacatctc gggatggaaa tgcgggatac tgatctgcta
     5821 cgacaataat gtggtggaaa atgtaagggc cacggccctg ctgggtgctg agattatttt
     5881 tatgccgcat gtcacgatgt gcactccgtc gccacggccg ggttcgggat ttgttgatcc
     5941 tgagctctgg aagaacaggg cagtgaatcc ggaagctctc agagccgaat ttgacggccc
     6001 aaaaggcagg gaatggctta tgaaatggct tccctcaagg gcacacgaca acggcatata
     6061 tgtggtgttt tcgaacccga ttggaatgga tgatgatcag ctgaaaaacg gttgttcaat
     6121 gatcctcgat ccgttcggga acataatggc ggagtgcaga actctcggcg atgattttgt
     6181 tactgcaacc gtaactgccg ataagcttac ccaggccgga ggttacaggt atacgagggc
     6241 gcgccggccg gaattgtacc gcgatatact gggaaaggac cataaaccgg ttcagaaagt
     6301 cgcctggttg ccccggcagg attcctgagc cttaatatga aagggtgacc tgatcgcggc
     6361 tttttccggg ttcttcattc gttttttgtc aggccggtca caaatgagaa agatcatcca
     6421 tatcgacatg gatgcttttt ttgcatcgat agaacagctg gataatccgg ggctgagagg
     6481 gaaacccgtt gcagtaggcg gaagcggaga gcgccatgtt gttgctgccg ccagctatga
     6541 ggcaagaaag ttcggcgtcc gttcggcaat gccgtcactt accgcccggc gcctgtgtcc
     6601 cgaccttatt tttgtacctc acaggtttga caggtacgag gagatatccg cgatcatttt
     6661 cggcatcttc agggaatata ccgacagggt tgaacccctt tcgattgatg aagcctttct
     6721 cgacgtaacg catgacagga agggtatcgg ctctgccacc ctgattgcaa gggctgttaa
     6781 gcaggagata gaaaaaaaga cagggcttac tgcttccgca ggcatatcat acaataaatt
     6841 ccttgccaag atagcatccg acatcaggaa acccaacgga cttttcgtca tttcacccga
     6901 tgacgccatg aaattcattg acgaactccc cgttgagaaa tttttcggga tcggcagggt
     6961 aacagcggag aagatgcaca ggctcggtat tcataaggga tcggaactga aacaaatgga
     7021 tctcctgtcc ctcatcagga atttcggtaa ggcaggcagg ttctaccacg atattgtcag
     7081 gggaatcgat aaccggcccg tcgaggcatt taccgaacgc aaatcgatag ggacggaggt
     7141 tacctatgaa aaggacctta caagccagtt cgggatcatt gcggagctgt acagggtgga
     7201 aaaggaactt atggtacgcc ttgaaaatgc aggaacaacc ggaaggacgg tcacggttaa
     7261 ggtgaaattc tcggatttca ggcagataac caggagcagg accttgccct gctatatccg
     7321 tgattttgat acccttcaca gggaagtgac cgcgataaga aaatccatgg atctggaagg
     7381 aagcaggata cggcttcttg gggtcactgt ttcaaacctt gaaagcgatg accatgccga
     7441 ccgccaactg ctgctgttcg atgagaacgt tatcagctaa accggccgga tcagcttttg
     7501 ccgcagtctg gatgcgcatt catcttcgtt tccgggtttc gcaggggcag gggacagcac
     7561 gaaccttccg gtgcttattc tgtgtggcgc aactgcccgc gatcagcagg atgatcatca
     7621 tgacaagagc cacccggcgc catgtaatcc gtaatctcat ttcagaaatc tgtcatttgt
     7681 tcagttatct gtatttaccg aaggtatcat acagttaacg ggaaaaaatg aaattaattt
     7741 caaaacaatt catctacgga taaatatctt tctcctgtat cgtagcaaaa ggccattata
     7801 cgggaaccgg gcttcaggtc atcaattacc tggctgacgg ctgcaaggac agcacctgtc
     7861 gagatgccga caaacaagcc ttcatccctg gcagctctcc gtgcatacct gtacgattct
     7921 tcctttccca ccttgattat ttcatcgata agcgaagtat cgagaatggc aggaatgaat
     7981 cccgccccga ttccctggat agggtggggc ccaggtgtgc cgccgctgag tataggcgac
     8041 aattcgggtt cgaccgcata aactttcagg cggggaaatt ccttcttcag cagactggca
     8101 catcccgtaa ggtggccacc tgttcctact cctgcaaaca tgtaatcgat gccttcggga
     8161 aaatcgcgga gaatctccat ggctgtcgtg ttccggtggg ccttaacatt ggcctggtta
     8221 tcaaactgca ttggtatcca tgaacccgga atttcatctc tgagttgcct cgatcgctcg
     8281 atggcaccct tcatcccttt ttccctcggg gtgagtaaga attgcgcacc ataggcgctc
     8341 ataatctttc ttctttcagg cgacatggat tcgggcatga caaggataat cctgtagcct
     8401 ttcacggctg ccaccatggc cagaccgatc ccggtattgc ctgaagtggg ttctataatt
     8461 acacctcccg gctcgagcaa tccattcttt tcggcatcct ctatcatttc cagcgcaatg
     8521 cggtccttga tgcttcctcc cggatttgat ctttcgagtt tgagccaaac ttcggatcct
     8581 tcaccaaaaa ggcggtttat tctgatgtgc ggagtattcc cgatcagttc aagaattgaa
     8641 tttgctttca ttgaattcag cagataatga cggattatat gatgaaattg tagccttcag
     8701 gggagtcatt cttccctttt acaattattt cactcttgtg atatacaacg caaaacgggg
     8761 caacgctttc ggtgagccac acattacctc cgataatact gtgatggcct acaacggtat
     8821 tgcctcccag gatagtacag ccggcataca atatcacatt gtcctcaatt gtcggatgcc
     8881 ttttcctgga agccttgctc ttgtcgacgc tcagtgcgcc aagggttaca ccctggtaaa
     8941 cttttacatg gtttcctatt ctcgttgttt cgccgataac tatccctgtt ccgtgatcaa
     9001 tcgaaaaggg cgttcctatc acggcacccg gatgaatatc aatgcctgtt attttatggg
     9061 catattcagt gatcatcctg ggaagcagcg gaacagacat cctctcaagc tcgtgggcga
     9121 tcctgtaaca atatattgca aaaaagcccg gatatatggc aatgacctcc tcaagactcc
     9181 tggctgcggg atctgactca aacatgaagg atgcatcctt aagcaggata gtctttattt
     9241 caggcattcg gcccatcagc cggtcagtca gatccacggg atcatcggtc agtccactga
     9301 agataagtac aagcagatcc ctgactgtct tgtggcccgt atcagtgaat ggtgccgtaa
     9361 tgtcgcccgt gaatggaaat aaagtcttga acagcttttt tacggctatt tcaacacttt
     9421 tcttgtttgg tatagtcacc gcggattatt atttaaatgc attctcaaga tcaacaagaa
     9481 gatcatcaat atgttcaatt cctaccgaca gacggatcag gttatcagta acccctatct
     9541 tctttctgag accggccgga aacgccccgt gagtcattat tgccgggtaa tttgccagcg
     9601 attccacacc gcccagactg tcggcaagct ggaatatttt cagttttctg aagaacgcat
     9661 ttgcttcgtt aagtccccct tccatcctga acgatacgac cccgccaaat cctgtcattt
     9721 gttttttcgc aatggcatgt ccgggatgtt cttcaaggcc cggataaaac acttccgtta
     9781 ccttcgggat atttttcaga tgccgggcca cggcaaatgc attaagttca tgctgtttca
     9841 acctgattgc cagggttttc aaaccccgca aggtaagcca tgaatcgaaa ggggagggaa
     9901 cggctcccac ggacttctgt acaagcctga tcctttcata taccgcctga tcgttcagta
     9961 tcactgcccc gcctataaca tcagagtgac cgttgatata tttggtggta ctgtggacaa
    10021 caatatccgc accatgcagg aggggtttct ggaaataggg ggaagcaaaa gtgttgtcaa
    10081 ccaccgttaa ggccccgtga tctcttgctg tatctgctat cttcccgata tcaaatattt
    10141 tgagcagggg gtttgtgggc gattcaagcc aaaccatcct ggttgaaggc ttgaaagagt
    10201 tcagtaatga accgtagtcg gcgtagtcgg cgaaggtgaa agtgatgccc gccggttcaa
    10261 gtatttcctt tatcagccga tatgttccac cgtacatgtc ttcagggaca attgcatgat
    10321 cgcccggccg caagagagaa aacaccgcat gttcagctgc cattcccgat gagaatgaca
    10381 gggcatgcct gccttcttcc agggaggcaa ggcatttgtc aagaaccgct cgggtgggat
    10441 ttcccacgcg tgaatactca tagcccccgg gtttgtctgc ttcttccctg gaaaaggtag
    10501 aggtctggta aatcggaagg atggttgcgc ctgttgaagg atcggcttcc tgtccgaccc
    10561 agatggcctt ggtttcaaac ttcatggatc aatgctgaaa acgatttgtg caaaattaat
    10621 catattcgcc acaaatcatt cctgcaccga cagtttcgtt ggttccttct tcaataaaga
    10681 tcatgctgcc ggttatgttg ttatttttat aacagtcata ataaaggggc cttggagtcc
    10741 tgatgctgat attggcaata tcattcatgt taacgtcgtt accggcggtt tccctttcaa
    10801 gattgcttat gttcatccgg taattaatgg aactgactac acaggtggtt tcattcgtat
    10861 tgttccttat caggtatttc cggccctgtt gcagcggttt ttcgttgaac cagcagacca
    10921 tcaggttaat atccacgcct gactctggca tttcaccctt tttcactatc atgtcgcccc
    10981 tgcttatgtc aatctggtcg gtgagattca gggtgacaga atctcccgcc tcagcttttg
    11041 aaatggtttt acccatcacc gtgatcgagg atatttccga ttccattccc gaaggaagaa
    11101 cggtaatcct gtcacccgca gagaatgttc cgctggccac cctgccggca tatcccctgt
    11161 aatcgtggta ttctgatgaa aggggccgga tcacggtctg aaccgggaat cttgcgggtc
    11221 ccgaattcct tttcttaccg atctctattg tttcaatcag gttgagaaag gtttttcctc
    11281 cgtaccattt catattttcc gacggactta caacattgtc accgtacttt gcgctaatag
    11341 gtatgaaatg caggtcgccg gtgccaagcc ttgatcccag tttcttcaga tcgtcgcaaa
    11401 gcctcaggta cacatcctcc gaatatccga caagatccat cttgttgatg cagaatacaa
    11461 tatgtcgaat gtcaagcagg ctggcgatat aggtgtgtct cattgtctgt tcaaccactc
    11521 cttttctcga gtcaatgagg atcaccgcaa ggtcggccgt gcttgcacct gttaccatat
    11581 tccttgtgta ctggatatgg ccgggtgtat cggcaatgat gaatttcctt gcgggagttg
    11641 caaagtacct gtatgcaacg tcaattgtga tgccctgttc cctttcagcc cggagtccgt
    11701 cggtgagcag cgacaggtcc gtttcctcct tgcccagtcg tttgctcgac tgaacaatgt
    11761 gctccatctg atcctcgaaa atggatttgc tgtcgtacaa aagacgtccg ataagtgtgc
    11821 ttttcccgtc gtcgacactc ccggcagtgg taaatctgag aagcccgatg ggccggtttt
    11881 caccattgag tttcatttgt atccggatga gatttgaata aattaccctg tgttcccggt
    11941 catcagcatg atccgggcaa aagtggccag tatatcctgt gggatcgatt tgcagttaaa
    12001 agtaaccttc ctttttcctg tcttccatgg ctgcttcaga tcgcatatca tcatatcttc
    12061 cgcctctttc tgttaccctt gtggcggcaa tctcggaaat aatctcttca agcgatccgg
    12121 ctttcgacat ggtgaccccg gtacaggtta tgtcgccaat ggtccggcat ctgatgtcgg
    12181 cttcaaaaac cttctcagat ggtttcaatt gcatgaacgg tgcccaggcg agataaaccc
    12241 cgttcctgag aaagatcttt cgccggtgcg tgtagtacag catgggcagc cggataccct
    12301 ccttcaggat gtattgccac acatcgagtt cggtccagtt actgatggga aaaaccctga
    12361 aatgttcccc gaaattcttg tgcccgttga atatgttcca cagttcgggc ctctggtttt
    12421 ttggatccca ttgtccgaat tcattcctgt gggagaaaaa ccgctccttg gcgcgtgcct
    12481 tttcttcatc ccgccgcccg ccgcccactg caacatcaat ccttagctcc tcgatggcat
    12541 caagcagggt gacagactgg gccttattac ggctcgcatt gatacctctt tcctcggtaa
    12601 cccttccctg gtcaatggta tcctggacta gcctgacaat cagctcaaca ccaagctcat
    12661 tggccagatc atccctgaac ttaagggttt cctcgaaatt atgaccagtg tcaatatgca
    12721 acaggctgaa tggcatcttt gccggccaga atgcttttct tgcaaggtgc accagaacaa
    12781 tcgagtcctt ccctccggaa aaaagtatta ctttcttttc aaactgtgat gcaacctccc
    12841 gcattacaaa tattgcctca tcctcaagtt ccttgagatg attgtcattt attgccagtg
    12901 tctgttcctt cattgcatta tcattccgtt tgctgcaaac tgaattatcc atgttttcct
    12961 gaatttcctg atcccgatat tctattttaa atgcagcccg catt
//
LOCUS       DAOOYG010000074        12567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_5204_length_12567_cov_6.280930, whole genome shotgun sequence.
ACCESSION   DAOOYG010000074 DAOOYG010000000
VERSION     DAOOYG010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12567
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5204_length_12567_cov_6.280930"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PK288_07485"
     CDS             <1..230
                     /locus_tag="PK288_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18804.1"
                     /translation="YYLNDALYSLPVTATSLTPSRVVDQLFYGVEVDPQTGNIVCLDA
                     VGSRAVVYNAAGIRQFEFETAKFPNSVTFSY"
     gene            435..1361
                     /locus_tag="PK288_07490"
     CDS             435..1361
                     /locus_tag="PK288_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPM18805.1"
                     /translation="MPEIVKYEGLVAAPFTPMDEKGNLNTGLIPVYYKFLEKNGITAV
                     FINGSTGEGASLSQREKELNASVWAGCMKNGGKMRVINLVGGTSYTECIENAIFSFEA
                     GISAVALVAPYYFKPSGAGQLAEFVARVGESVPGMPVYFYHIPVLTGVNLPMHDFLKR
                     ISGMLDNFAGIKFTHEDFMDFLLCLNFSGGRYDLLWGRDECTLSALVLGCRGFVGSTF
                     NYAAPLYHEMIRAYDGGDLEKARMLQLKSIEMILLLSKYGGIATGKAYMKLRGINAGQ
                     FRLPVRNMSDESYMAFKRDVENLGMGEYFSTL"
     gene            1533..2780
                     /locus_tag="PK288_07495"
     CDS             1533..2780
                     /locus_tag="PK288_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM18806.1"
                     /translation="MSLKNKSWYPWLIVGLLWGVALLNYMDRQMLSTMRIAIMTDIRE
                     LNEAENFGRLMAVFLWIYGLMSPAAGLIADRINRKWLIIGSLGVWSSVTLGMGFATTF
                     SQLYVLRAVMGVSEALYIPAGLALITDYHRGNTRSLAIGIHMTGLYTGQALGGFGATV
                     AHAWSWHTAFQSFGIIGIIYSIVLMLFLAEKRPEQAVEKRAIRISSGLGSVGKSLGLL
                     FGTISFWVILFYFAVPSFPGWAIKNWLPTLFSETLKTEMSVAGPASTITIALSSLLGV
                     ITGGIIADRWIMRNLKGRIYTGAIGLSLTVPALFLVGYGNWLPAIMGGSILFGIGFGM
                     FDANNMPILCQFVSPRHRAAGYGLMNMTGVFAGALITEWLGRSTDAGNLGKDMALLAI
                     PVAAAVILLLVTLKPKYADKTSD"
     gene            complement(2825..3457)
                     /locus_tag="PK288_07500"
     CDS             complement(2825..3457)
                     /locus_tag="PK288_07500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPM18807.1"
                     /translation="MFSVIIAAYNRARLIERAIKSLLMQTETDWEGIIVDDGSTDGTY
                     SVIAPYLEGETGIRYFRIPHGGESRAKNYGIGVSEGRYITFLDSDDEYHPDHLSERRR
                     YLAENPGVMFLYGGVRILGNQFVPDRNNPSAVINLKDCVIGGTFIIERDVLAALEGFR
                     DILLGPDSDLFQRAVEKGIPMAEIDKPTYIYHHENPNSITNVLLKKKKGF"
     gene            complement(3675..4568)
                     /locus_tag="PK288_07505"
     CDS             complement(3675..4568)
                     /locus_tag="PK288_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPM18808.1"
                     /translation="MEHSHTDSHDVKLGNAFKTGIIINVIFIVIEVIYGFLANSMALV
                     ADAGHNLSDVLALIFSWMALIVSQRKPTLKFTYGFRRSTILTAMLNTILLLVAVGFIA
                     WETISRIGRPMEINTGKVIFVASAGIVVNGFTAWLFMKDKRNDLNVRSAFLHFLADAL
                     VSAGVVVAGVIIALTGIVWVDSVVSFLIIAVILYSSYHLLIDSISLSLDAVPENINIE
                     EVRNYLGSLPEVTGVHDLHIWALGTTAAALTVHLNTKVPTGVDFISALQKQLHEKFDI
                     EHSTIQIEYGESMSECVSNCN"
     gene            complement(4582..6414)
                     /locus_tag="PK288_07510"
     CDS             complement(4582..6414)
                     /locus_tag="PK288_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPM18809.1"
                     /translation="MRILKSFNESSVKLLKYGIFLVLGLLLGRIFFHSREVRETLHDH
                     QTEVEQATVWTCSMHPQIRMDHPGKCPICGMDLIPLLKGGSLHTDPDAIHLSKEAAEL
                     ANVLTTKVSWQKPVRQIRLYGKVQIDERLFQSQVAHVPGRIEKLLVNFTGEQVRKGQK
                     LAEIYSPELVNARQELIETAATRKLYPEFYEAAREKLRKWKLTDEQIEALENSGPATD
                     NFDVLSNTSGTVRARRVSTGDYVSQGTVLFDIADLSRVWVEFDAYETDLPFIRRGDKV
                     DFTVRAIPGSVFSGNIVFIDPVIDPVTRVAKVRVEAANVSGKLKPEMYATGTVSSDLK
                     EYSNNLVIPGSAVLWTGKRSIVYVKDPGEEEPVFRMRVIELGPALGDSYIVTDGLKEG
                     EEIVTRGTFSVDAAAQLEGKPSMMNPPDNAAAMLVHGMGAGKPGPDNTETLSDRKLKD
                     DAKDAPAKINVSSDFKKQLTSVLDRYLSLKNSLVESDPEKVKQEAEKLKQAFLKSDLK
                     PLPENDRLQWNTLSDKLLRNVTELIASDGLMNQRLAFQKLSNNLYIAIKTYGLAGKTL
                     YYQFCPMFNNNNGGYWLSETGEIRNPYFGEEMLSCGETRENLDF"
     gene            complement(6425..7834)
                     /locus_tag="PK288_07515"
     CDS             complement(6425..7834)
                     /locus_tag="PK288_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPM18810.1"
                     /translation="MTGPAYSFGQQIPDSLLKYMEVAAANNPEVMQRLAEYEASLKKI
                     PQAGTLPDPQLELGVFLTPMEIVSGLQMADLRLMQMFPWFGVLRNARDEMSLMAKAKF
                     ELFRDSKLQVCYEVQKAWYELFRIRKEKDISEKNIEILRIIEQLTLTSFSAPEAEQSG
                     SAGGQSGAEEQTSPAVPSGPGNGMRGMGANQSARGAMSTGQAGSSMQSGSMGSSAGSS
                     GLTDLYRIRIETGELQNRIASLNDRERVVVAQFNGFLNRPPSTYVYTADVLIPDTLHP
                     YLSVIPDSLLNNNPMLGMLSFEQEAYKSRKKMVTGMGFPMVGLGLSYSLVGRSSMSTS
                     AMNGMDMIMPMVSVTVPVYRKKYRAMKEEADLLGKAGSYSLQAASNSLNSEYYSAIQL
                     YNDARRRMELYYDQYELASRSFEIMLKAFRASTAPLADVLRARQQLLDYELKQVEAIT
                     DFGTASAWLNRLAPASKFN"
     gene            complement(7873..11718)
                     /locus_tag="PK288_07520"
     CDS             complement(7873..11718)
                     /locus_tag="PK288_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPM18811.1"
                     /translation="MFNRLVKYFLENRLITFILFGAFIVTGLVTMPFNLKTSVLPREP
                     VPVDAIPDIGENQQIVSTEWMGRSPRDIQDQVTYPLTTALLGIPGVRTIRSTSMFGMS
                     FIYIIFNDNVEFYWSRSRILEKLNSLPPGTLPAGVQPSLGPDATALGQVFWYTLEGRN
                     PETGQPAGGWDPQELRSIQDFYVRYGLSAAEGVAEVASVGGYIKEYQVDLDPGALKAF
                     GVSVMDVMDAVKLANLDAGAETMELNNVEYIIRGLGYVKSLEDLELSVVTVKNGVPVR
                     VKDVAKVGFGPATRRGGLDKSGAEAVGGVVVARYKSNPLEVINSVKARISEIESGLPQ
                     KTLPDGTVSKVTIVPFYDRTGLIRETLGTLETALSHEILISIIVIIVLVLNLRASLVV
                     SGLLPAGVLMTFTLMRLSGVDANIVALSGIAIAIGVMVDIGIVDVENILRHLEMPENR
                     GLKGKQLLNVIYLATTEVRAAVVTSVATTVVSFLPVFAMQAQEGKLFHPLAYTKTFAL
                     LSAFILGIVVLPTMVHILFNVSFDRKRIRRIWNIILIPVGLVISAVWHFWPALALSAV
                     GLNNLFGYRWGTGREKYPDYINIIITVLFATWYLAKGWLPLGARNSELSNFLFVAGTV
                     SVILIALMSMVHFYENILTWALENKRKFLLIPAFTLIFGLLAWQGFDRVAGFVAAGFE
                     KAGWKKIRQTHFWQNAAGLFPGAGKEFMPALDEGSFLLMPTSMPHSGIEKNLEYVKTL
                     DLRLSAIPEVESAVGKWGRVNSALDPAPVQMFENTINYRPEYILDENGRRMRFSTDRE
                     GNFILKGGVKYNPANEKFRVIPADSLIPGKKGKYFRQWRPDIRKSDDIWKEIVKVTDI
                     PGLTSAPKLQPIETRLVMLSTGLRAPMGLKVYGPDLASIELAGTQFEKALKEVPSVNP
                     LTVFYDRAIGAPYLEIRLDREAMARYGMTVSGVQDVLQAAVGGMALSTSVEGRERFPL
                     RVRYARELRDSPEEIEKILVPALNGTQIPLGEIADIVYTRDAQMIQSENTFLVGYVIF
                     DKHEGKAEVDVVNEAGKILKSGIDSGDIVLPAGVTYKFAGNYENQVRATRRLAVVIPV
                     SLIVIFLLLYFQFRTVTASLIHFSGVFVAFSGGFIMLWLYGQDWFMNFSVAGLNMRDL
                     FQMHTINLSVAVWVGFIALFGIATNDGVIMGTYIHQVFEEHKLTTVHEVRQAVLTAGK
                     KRIRPAMMTTAVTVIALLPVLSSSGKGADIMVPMAIPAFGGMIIQVMTVFVVPLFQAI
                     WRENALKHHTLVSVNQIEK"
     gene            complement(11778..12149)
                     /locus_tag="PK288_07525"
     CDS             complement(11778..12149)
                     /locus_tag="PK288_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPM18812.1"
                     /translation="MKSLNLFLAVLLASVLSSPGPAQTRTDGSTGSVSARTESFKVWG
                     KCDMCKTRIEKTAKEAGATSASWDGKTQLLTVTFDPSVTGRDAISKKLASVGHDTEKY
                     KAPDDVYAKLPGCCHYERAKQ"
BASE COUNT         3190 a         3220 c         2868 g         3289 t
ORIGIN      
        1 tttattatct caacgatgcc ctttattccc tgcctgtgac tgcaacctcc ttaacgccat
       61 ccagggttgt tgaccagtta ttctatggag tggaagtgga tcctcaaaca ggaaatatcg
      121 tctgcctcga tgcagtcggt tccagggctg ttgtttacaa tgcagccgga atccggcagt
      181 tcgaattcga aactgcaaaa tttcctaatt ccgtgacttt cagttactaa ccctccttct
      241 gcaacaaggc ttaaacaccg gttcggagta aagggaacaa cacatatttt ccggatatca
      301 ttgcacgctc tgccggaaaa gatttttttc cggtaccata tccgcaggtt acaccgtagt
      361 tgtttcaacg ttttttcagt ctgttgcagg atcatcggaa aaacaatatt tttagaaatg
      421 aaaaattaca gatcatgcct gagatagtca aatacgaggg tcttgttgca gctcccttca
      481 caccgatgga tgaaaaggga aatctcaata ccggacttat tccggtgtat tacaaattcc
      541 tggagaaaaa cgggataaca gctgtgttca taaacgggtc aaccggcgag ggtgcctcat
      601 tgtcgcaacg ggaaaaggag ctcaatgcct ctgtatgggc cggctgcatg aagaacgggg
      661 gaaagatgcg ggtaataaac ctcgtcgggg gaacctcata caccgaatgc atcgaaaatg
      721 ccattttctc atttgaggca ggcatttctg ccgtggcact cgttgctcct tattatttca
      781 aaccctccgg ggccggtcag cttgccgaat ttgttgccag ggtaggagaa tccgtacccg
      841 gcatgcctgt ctatttctat catattcctg tacttaccgg agtgaaccta cccatgcatg
      901 atttcctgaa aagaatatcg ggaatgcttg acaattttgc cggcataaaa ttcactcatg
      961 aggattttat ggacttcctg ttatgcctta atttcagcgg agggcgctac gaccttctct
     1021 ggggaagaga tgaatgtacg ctctcagccc tggttctggg ctgcaggggt ttcgtgggca
     1081 gtaccttcaa ctatgctgcg ccgctttatc atgaaatgat aagggcatat gacggcggcg
     1141 acctggaaaa agccaggatg ctccagctta aatcaattga aatgatattg ctcctgtcaa
     1201 aatacggagg gatagcgacg ggaaaagctt acatgaaact taggggaatc aatgccggac
     1261 agttcaggct tcctgtacgc aatatgtcag acgaatctta tatggctttt aagcgggacg
     1321 ttgaaaattt aggcatggga gagtactttt caactttgta agctcaacgt cctgtcacac
     1381 aattgttata aaatgctata acgtatggaa ggaaatctat ttcgtctatc ccgggtcctg
     1441 tccggaacaa taaagctgcg gatgacaccg ggcaattgat aattttaatt tcaggatcag
     1501 atattgttgt cgaaccataa aactgaaaaa tcatcagcct taagaacaaa tcctggtatc
     1561 cctggcttat agtggggctt ctttggggag tggcactact caactatatg gatcgtcaga
     1621 tgctgtcgac tatgaggatc gccatcatga ccgacatcag ggaactcaat gaagcagaga
     1681 actttggccg gcttatggct gtttttctct ggatttacgg gttaatgtcc ccggcagccg
     1741 gactgattgc cgatcgcatc aaccgcaaat ggctcataat agggagcctc ggagtatggt
     1801 cgtcagttac tctgggaatg ggatttgcaa ccaccttcag tcagctgtat gttctgcggg
     1861 ccgtcatggg agtaagtgaa gccctgtata ttccggcggg ccttgccctg ataactgatt
     1921 atcaccgggg gaatacgcgt tcactggcca tcgggataca catgacaggt ctttacaccg
     1981 gtcaggccct cggcggattc ggggcaacag tagctcatgc ctggtcgtgg cacacagcct
     2041 ttcaaagttt cgggatcatc ggcattattt acagtatcgt gctgatgctt ttccttgcgg
     2101 agaaaaggcc ggagcaggcc gtcgaaaaaa gagcaataag gatttcctca ggccttggat
     2161 cagtcggaaa aagtctcggg ctcctgttcg gaacgataag cttctgggtg atactctttt
     2221 attttgcagt cccaagcttc ccgggttggg caataaaaaa ctggctccct accctgttct
     2281 ctgaaaccct gaaaacagag atgtcagttg ccggaccggc aagcactata accatagcat
     2341 tgtcatcatt gctcggagtg ataacggggg gtatcattgc cgatcggtgg atcatgagga
     2401 atctgaaagg gaggatctac accggggcaa taggactttc actcaccgta ccggcccttt
     2461 tcctcgtcgg ttacgggaac tggcttcccg cgataatggg cggcagcata cttttcggca
     2521 ttggtttcgg gatgttcgac gcaaacaaca tgccgatact ctgccagttt gtttcaccgc
     2581 ggcaccgggc agcaggttac gggctgatga acatgaccgg agtgtttgcc ggcgcattga
     2641 tcacagaatg gcttggcaga tcgaccgatg caggcaacct tggaaaggat atggccctgc
     2701 tggctattcc cgttgcagct gcagtgatcc tgctgcttgt cacgctgaaa ccaaaatatg
     2761 cagataaaac cagcgattag gaaagccggc aggcaggatt ttccttccgg gtgaaatatg
     2821 ccgctcagaa accttttttt ttcttaagaa gaacattcgt tattgaattg ggattctcat
     2881 gatgatagat gtaggtcggc ttgtcgattt cagccatagg gatacctttt tccaccgccc
     2941 tttgaaacag atcactgtca gggccaagaa gaatatccct gaatccttca agggcggcaa
     3001 ggacatctct ttcaataata aacgtgcctc caatcacaca atccttcaga ttaataactg
     3061 cagagggatt gttcctgtcg ggcacgaact ggttacccag tatccttacg ccgccataaa
     3121 ggaacatgac accgggattt tccgccagat atctacgcct ttctgacaga tgatcaggat
     3181 gatattcatc atccgaatcg aggaaagtta tatatctccc ttcagaaact cctataccgt
     3241 aattctttgc acgggattcg ccgccatgcg gtatcctgaa atacctgatc cctgtttccc
     3301 cttccaggta tggggcaatt acagaatagg tgccatcggt gcttccgtca tcgacaatta
     3361 tcccctccca gtctgtctca gtttgcatca gcagcgattt aatggctctc tcgatgagcc
     3421 gcgccctgtt atatgcagca attattaccg agaacattgg ccgggagtat atgtttcggc
     3481 ttttgtcatt ctgattttcc tttccccgga tcatacctgc gattcttcat gttgttttca
     3541 atgacagcct gaactcatgg ataatccgca ccggaattga tgcattaacg ggcagggatc
     3601 ttttcaacgg ctgcagattg atccgccagg tgttacaata tagggaaaaa acatataccc
     3661 cggtccgccg gaactcaatt gcagttactc acacactcag acatgctttc cccgtattcg
     3721 atctgaatgg ttgaatgttc aatgtcgaat ttttcatgca attgtttctg gagtgccgag
     3781 ataaagtcaa cgccggtggg tacctttgtg ttcagatgga cagtgagggc agcagcagtt
     3841 gttcccaggg cccagatatg cagatcatga accccggtta cttcaggtaa gctgccaaga
     3901 taatttctga cctcctcaat attgatattt tcaggtactg catcaagcga aagacttatg
     3961 gaatcgataa gcagatgata tgaactgtat agtattacgg caattattaa aaatgaaacg
     4021 acagaatcca cccatacaat gcctgtcagt gcaatgatca ccccggccac aaccacaccg
     4081 gccgacacca gtgcatctgc caggaaatgc agaaatgcac ttcgcacgtt aagatcgttc
     4141 cttttgtctt tcatgaacaa ccaggcagtg aatccgttca ccacgatgcc tgcggacgct
     4201 acaaatatta ccttgcccgt atttatttcc atgggtctgc cgatcctgga aatggtctcc
     4261 catgcaataa atcccactgc aaccagcagt aatatggtat tcagcattgc tgtgagtatc
     4321 gtcgatctcc tgaatccata ggtgaatttc agggttggtt tgcgctgtga cacgatcaat
     4381 gccatccacg agaatataag tgccagcaca tcactcagat tatggcccgc atcggcaacc
     4441 agggccattg aatttgccag gaaaccgtat ataacctcaa ttacgataaa aataacattg
     4501 ataataatgc ctgttttaaa ggcattgccg agcttaacat cgtgactgtc ggtatgagaa
     4561 tgttccatcg gccgttattc atcaaaaatc caggttttcc cttgtctctc cgcaactgag
     4621 catttcctcc ccgaaataag gattgcggat ctccccggtt tcactcagcc agtatcctcc
     4681 gttgttattg ttgaacatcg ggcagaactg gtaatacagg gttttacctg ccagtccgta
     4741 agttttgatg gcaatataaa gattattgct taatttctga aaagccagcc gctgattcat
     4801 aagtccatct gaagcaatca attccgtaac attcctcaga agtttgtcag acaatgtatt
     4861 ccactgcaat cggtcgtttt ccggaagggg tttcaggtcg gatttcaaaa aagcctgctt
     4921 cagcttctct gcctcctgtt tcaccttttc gggatcgctt tccaccagtg agttcttaag
     4981 actcagatac cggtcaagaa cactggtcag ctgtttttta aaatcactgc tcacattgat
     5041 cttcgctggt gcatcctttg cgtcatcttt cagcttccgg tcagagaggg tttcggtgtt
     5101 gtccgggccc ggctttcccg ctcccatacc gtgtactaac atggctgcag cattgtcagg
     5161 cggattcatc atgcttggtt tgccttcaag ctgtgcagca gcatccacgc tgaatgttcc
     5221 ccgggtcact atctcttcac cttccttcag accatcggta acaatataac tgtcgccaag
     5281 ggccggaccc aattcgatta ccctcattct gaaaacaggt tcttcttccc cgggatcctt
     5341 aacatacact atggatcgtt tacctgtcca aaggacggcc gacccgggaa taacaagatt
     5401 attgctgtac tccttcaggt ctgatgacac tgttccggtt gcatacattt cgggtttcag
     5461 ctttcctgaa acattggccg cctccacccg gacttttgca accctggtaa cggggtcgat
     5521 caccggatcg ataaacacta tgttccctga aaaaactgaa cccggtatgg cccggacagt
     5581 aaaatcaacc ttatcgcccc gcctgataaa gggaagatcg gtttcatagg catcgaactc
     5641 cacccataca cgtgaaaggt cagcaatgtc aaaaagcacc gtaccctgcg aaacataatc
     5701 ccctgtgctt accctgcggg cccggactgt tccggaagtg ttcgacaaaa catcgaaatt
     5761 gtccgttgcc ggcccggaat tttccaatgc ttcaatctgc tcgtcagtca gcttccattt
     5821 acggagtttt tccctggccg cttcatagaa ttcaggatac aacttcctgg ttgcggctgt
     5881 ttcaatcaat tcctggcgtg cgttcaccag ctccggagaa taaatctcag cgagtttctg
     5941 tcccttcctc acctgctcac ctgtaaagtt cacaaggagt ttttctatcc ttcccggcac
     6001 atgggcaacc tggctctgga aaagtctttc atcgatctga acttttccat aaagcctgat
     6061 ctgtctgaca ggcttctgcc acgacacttt cgtggtaaga acattggcca gctctgctgc
     6121 ttccttcgac aggtgaattg catccggatc agtatgcagg gaacctcctt tcagaagagg
     6181 aattagatcc attccgcaga tgggacattt gccagggtga tccattctta tctggggatg
     6241 catggaacag gtccaaactg tagcctgttc aacctccgtc tgatgatcgt gcaatgtttc
     6301 cctgacctcc cgggaatgga agaagatccg cccgagtaat aggcctagga caagaaagat
     6361 cccgtatttc agcagtttga cggaggattc attgaatgat ttcaggattc tcattgttct
     6421 tcaattaatt gaatttagaa gcaggcgcaa gcctgtttaa ccaggcgctg gccgtgccaa
     6481 agtctgtaat tgcctcaacc tgtttaagct cataatcaag aagctgctgc cttgcccgta
     6541 aaacatcagc aagaggagca gtggatgccc ggaaggcttt gagcattatc tcaaacgacc
     6601 tggatgcaag ctcatactgg tcataataaa gttccattct cctcctcgca tcattgtata
     6661 gctggatggc gctgtaatat tcactgttaa gtgaattgga agcagcctga agactgtagg
     6721 agccggcctt gcccagcaga tcagcttcct ccttcatggc cctgtatttt ttcctgtaaa
     6781 ccggaacggt aacactgacc atcggcataa tcatatccat accgttcatt gcagatgtgg
     6841 acatggagct tctccccacc aatgaataac tgagcccgag gcctaccatg ggaaagccca
     6901 ttcctgtcac catttttttt ctcgacttgt aagcttcctg ttcaaagctc agcataccca
     6961 gcatcggatt attattcagg agtgaatcgg gaatgactga aagatatgga tgaagcgtgt
     7021 caggtattaa gacgtcagcc gtataaacat atgttgacgg cggcctgttg agaaaaccgt
     7081 tgaattgtgc aactaccacc cgttccctgt cattgaggct ggctatcctg ttttgcagtt
     7141 caccggtttc tatcctgatc ctgtaaagat cagtaagacc tgaactgcca gccgaagatc
     7201 ccatcgaccc tgactgcatg gagctgccgg cctgccctgt ggacatggca ccccgggcag
     7261 actgattggc tcccattcca cgcatcccgt tccctgggcc cgaaggtacg gcagggcttg
     7321 tttgctcctc tgctcccgat tggccgccgg cggatcctga ttgttccgct tcgggtgctg
     7381 aaaaactggt aagggtcagc tgttcaatta tcctgagtat ttcaatattc ttttctgaaa
     7441 tatctttttc tttccgtatc ctgaatagct cataccatgc cttttgcacc tcataacaga
     7501 cctgcagctt tgaatccctg aacaattcga attttgcttt tgccataagg ctcatctcat
     7561 cccttgcatt cctcagtacc ccgaaccacg ggaacatttg cattagtcta aggtcggcca
     7621 tctgtaaccc tgaaacaatt tccatcgggg taagaaaaac cccgagctcc agttgcggat
     7681 cgggaagggt gccggcctgc ggtatttttt tcagggaagc ttcatattcg gcgagtctct
     7741 gcattacctc aggattatta gccgcagcaa cttccatata tttcagtagt gaatcgggga
     7801 tctgctggcc gaaggaatat gcaggacctg tcatcaatac ccagattaaa aacacaggtt
     7861 taattaaagc tctcattttt caatttgatt aacagatacc aatgtgtgat gtttcagggc
     7921 attctccctc catattgcct ggaaaagcgg aaccacaaaa acagtcataa cctgtatgat
     7981 cattcccccg aaggccggaa tggccatcgg aaccataata tcagcgcctt tgcctgaaga
     8041 tgagagcacc ggcagcaggg caattacggt taccgctgtt gtcatcatgg caggccggat
     8101 cctctttttg cccgctgtca gcactgcctg cctgacttca tgaacagtgg tgagtttgtg
     8161 ttcttcgaaa acctggtgga tatatgtacc catgataacc ccgtcatttg tggcaatgcc
     8221 gaataaggca ataaatccaa cccaaacggc aacgctgaga ttgattgtat gcatctggaa
     8281 aaggtcgcgc atgttgagtc cggccactga gaaattcatg aaccaatcct gtccgtaaag
     8341 ccatagcata ataaatcctc ccgaaaaagc tacgaacaca cctgagaaat gaatcaatga
     8401 tgctgtaacg gtacggaact ggaagtataa aagaagaaaa atcacaataa gactgaccgg
     8461 aataacaaca gcgagccgcc tggttgccct gacctggttt tcgtaatttc cggcgaattt
     8521 atacgtcacg cctgcaggaa gtacgatatc acccgaatca attcccgatt tcaggatctt
     8581 tccggcttca tttacaacat ccacctccgc cttaccctca tgcttgtcga agatcacgta
     8641 gcccacaagg aaagtgttct cgctctggat catctgtgca tcgcgcgtat aaactatgtc
     8701 tgcgatttct ccgaggggga tctgggtccc gtttaatgca ggaacaagaa ttttttctat
     8761 ctcctccggg ctgtccctga gctcccttgc gtagcgcacc ctgagaggaa acctttccct
     8821 gccctcaacc gatgtcgata aagccatgcc tcccacagca gcctgaagta catcctgtac
     8881 tccgctgacc gtcattccat atcgcgccat cgcttcccgg tcgagcctga tttcaagata
     8941 cggggctcct attgcccggt cgtaaaatac tgttaaagga ttaacagatg gtacttcctt
     9001 aagggctttt tcaaactgcg tcccggccag ttcaatggat gcgagatcgg gaccgtacac
     9061 tttgaggccc atgggagcgc gcaaacccgt tgaaagcatt accagcctcg tttcaatcgg
     9121 ctgcagtttc ggggcagatg tcaaacccgg aatatcagtc acctttacta tctctttcca
     9181 gatatcatcg gatttcctta tatccggccg ccactggcgg aaatacttgc ctttcttccc
     9241 cggaataaga ctgtcagccg gaatgaccct gaatttttca tttgccggat tgtatttaac
     9301 gcctcccttc agaatgaaat tcccttccct gtctgtgctg aaccgcatcc ttcgcccatt
     9361 ttcatcgaga atgtattcag gccggtaatt gatggtattt tcaaacatct gtacaggtgc
     9421 cggatcaagg gccgaattca ccctgcccca tttgccaacg gccgactcga cttcgggaat
     9481 ggccgaaagc cgcaggtcga gggtttttac atactcaagg tttttttcga ttcccgaatg
     9541 gggcatgctc gttggcatca gcaaaaagga accttcatcc agcgcgggca tgaattcctt
     9601 tcccgccccc ggaaagagtc ctgccgcatt ctgccagaaa tgcgtctgcc ttatcttttt
     9661 ccatccggct ttttcaaagc ccgcggcaac aaaacctgca acccggtcga agccctgcca
     9721 tgccagcaac ccgaaaatca gggtgaatgc cgggatcagc aggaattttc gcttgttttc
     9781 cagggcccat gtcagaatgt tctcataaaa atgaaccatt gacataagtg caatgagtat
     9841 gaccgaaacg gtccctgcca caaaaagaaa attggaaagc tcactgttgc gggccccaag
     9901 cggcagccat ccctttgcaa gataccatgt ggcgaaaagg acagtaatga ttatattgat
     9961 gtaatcaggg tacttttccc tccctgtacc ccagcggtaa ccaaaaagat tgttcagacc
    10021 gacagctgac agggcaagcg cgggccagaa gtgccagact gctgatatga ccagtccgac
    10081 cgggatcaga ataatattcc atatccgcct gatcctcttc ctgtcaaatg aaacattgaa
    10141 aagaatatgg accatggtgg gcagtactac aatacccagg ataaatgccg acagaagtgc
    10201 gaaggttttt gtgtatgcaa gaggatggaa caattttcct tcctgtgcct gcatggcaaa
    10261 gaccggaaga aagctaacca ccgtggtggc aacagaagtt accactgcgg ccctgacctc
    10321 ggtggtggca aggtaaatga cgtttaacag ctgtttacct ttcaatcccc ggttctcagg
    10381 catttccagg tgacgcagta tgttctctac atcaacaatc ccgatatcca ccatcacccc
    10441 gattgcaatg gcaatacccg acaatgccac aatattcgca tccacgcctg acagcctcat
    10501 cagtgtaaat gtcatcagca cccctgcagg cagaaggccg gaaaccacca gggaagccct
    10561 caaattaaga acaagcacga ttattacaat gatacttata aggatctcat gcgacagggc
    10621 agtttcgagc gttcccaggg tttccctaat aaggccggtt ctgtcataga atgggactat
    10681 tgttaccttc gaaacggtac catcgggcag tgttttttgc ggcagtccgg attcaatttc
    10741 acttatcctg gctttaacgc tgttaatcac ctcgagcgga ttcgacttat atcgcgccac
    10801 gacaacgcca ccaaccgcct ctgctcctga tttgtcaagg cctcccctac gggttgcagg
    10861 cccgaaacca accttcgcga catcttttac cctgacagga acgccgtttt tcactgtcac
    10921 aactgaaagt tcaagatctt caaggctttt gacatatccc agtcccctga taatatattc
    10981 gacattgtta agctccatgg tttctgcccc ggcatccaga ttcgccagtt ttactgcatc
    11041 cattacatcc atcactgaca ccccgaatgc tttgagggca cctggatcga gatcgacctg
    11101 gtactccttg atatatcccc cgactgaagc gacttcagca accccttcag ctgctgacag
    11161 tccatacctg acataaaaat cctggatcga tcttaactcc tggggatccc agccgcccgc
    11221 cggctgtcct gtttccggat tcctgccttc caatgtatac cagaatacct gtcccagggc
    11281 tgttgcatca ggtccgagag agggctgaac gccggccgga agtgtccccg gaggaaggga
    11341 attcagcttt tcaagtatcc tcgacctgct ccagtaaaac tcaacattgt cattgaaaat
    11401 gatgtatatg aaggacatgc cgaacattga cgtgctcctt attgtccgga cccccgggat
    11461 ccccagcagg gcagtggtaa gcggatatgt tacctggtcc tggatatccc tgggtgaacg
    11521 ccccatccat tcagttgaga cgatctgttg attctcaccg atatccggaa tagcatcaac
    11581 gggaaccggt tcacgtggca ggacggaggt tttcagattg aacggcattg taacaagccc
    11641 ggtcacaatg aaagctccga ataatataaa ggtgatgagc ctgttttcaa gaaaatattt
    11701 taccagccgg ttaaacatgg tcacggaatg ttaatcgcgg catgaagaag caggccggat
    11761 gcagtatgat cggcctttca ttgcttcgct ctttcataat gacagcaacc cggaagcttt
    11821 gcatatacat catcgggtgc tttgtatttt tcggtatcgt ggccgacaga tgccagcttt
    11881 ttgctaattg catccctccc tgtaactgag ggatcgaatg taacagtgag aagctgtgtc
    11941 ttgccatccc atgaggcgct ggtagcaccg gcttccttcg cggttttttc gatacgtgtc
    12001 ttgcacatat cacactttcc ccatacctta aatgactccg tcctggccga tactgaacca
    12061 gttgagccat ctgtgcgtgt ctgggcagga ccaggcgagg aaaggacaga agcaagcaaa
    12121 actgcaagaa aaagattcaa cgatttcatg ataagaattt ttaagagtta taaataccgg
    12181 atttgaatga atatgggaat tcggccacaa caggcttata aaattcagtg aacatgcctg
    12241 tcagaccgcg attctttcaa gctaaggcag attatcaggt acggagaatg taaatccccg
    12301 caatcggaat atccgttccc atcgcgggac cgggcggcaa aacccatgaa accggctcat
    12361 ttgtataaca ttgcctgaac agcatattta gaaaaaccgg atgctcgttc ccggggccgg
    12421 gatgctgcaa cctgaagtat actggaaggt acttgccgct gatattgtaa aacacgatct
    12481 gatcctcgca gcacctgctg ccggcaacag gttggtccgg caaagtgcag tctgcggttt
    12541 ccatgccgca cccgaccggc ttccccg
//
LOCUS       DAOOYG010000075        12490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_5264_length_12490_cov_7.198794, whole genome shotgun sequence.
ACCESSION   DAOOYG010000075 DAOOYG010000000
VERSION     DAOOYG010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12490
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5264_length_12490_cov_7.198794"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PK288_07530"
     CDS             complement(<1..664)
                     /locus_tag="PK288_07530"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HPM18813.1"
                     /translation="MNLKELTELLSEGSVEARLEKAASLFPGKVIFTTSFGLEDQAIS
                     HIIFSNSIDIEVVTLDTGRLFPETYKVFNETRKKYNKPIKVFFPDREDVEKMVTEKGP
                     YSFYYSKENRLECCHIRKVIPLNRALAGKSCWISGIRADQSDSRSKMHQVEHDVNRDL
                     YKFYPLFNWTYNQVREYVKLNNVPYNSLHDKGFVSIGCEPCTRAVQENGDFRSGRWWW
                     ENS"
     gene            complement(642..962)
                     /locus_tag="PK288_07535"
     CDS             complement(642..962)
                     /locus_tag="PK288_07535"
                     /inference="COORDINATES: protein motif:HMM:NF021362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HPM18814.1"
                     /translation="MLDVLINHSAVTDDQKGIIRVSERADKPIKSFMKSVSWRIVGTL
                     DTIIISYIITGKVTLAVSIGSIEVLTKTILYYFHERIWAHIHKIRLKINLKKRRGYEF
                     ERAD"
     gene            complement(1212..1457)
                     /locus_tag="PK288_07540"
     CDS             complement(1212..1457)
                     /locus_tag="PK288_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPM18815.1"
                     /translation="MAKTMTFNELRKLKDQLPSGSMKKIAEKLQLNEETVRNYFGGTN
                     FEKGDTVGVHFEQGPGGGIVSFDDTTILDLAKTMIGK"
     gene            complement(1577..2338)
                     /locus_tag="PK288_07545"
     CDS             complement(1577..2338)
                     /locus_tag="PK288_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HPM18816.1"
                     /translation="MNREKIRISAVSFANTYPFIYGLTQSGFDKKVILTTDHPAECAA
                     KLANGSADIGLIPVAALPMLDEYHLITDYCLGAYGKVRTVMLLSNTPFEDISIINLDY
                     RSRSSVSLARILAKNVWKKEFTWRDTSEGFDFLSIPYGEAVVLIGDLCFEYEKLFRFG
                     TDLAGEWFNYTGLPFAFACWTSNRKLDDSFLKDFNTALKAGVDNIPAVVEKYGTNGSI
                     RGNDLYVYLTRNMSFDLNDDKREAINLFLKLLKDL"
     gene            2459..3802
                     /gene="purB"
                     /locus_tag="PK288_07550"
     CDS             2459..3802
                     /gene="purB"
                     /locus_tag="PK288_07550"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPM18817.1"
                     /translation="MELNSLTAISPLDGRYRPKVEELDAYFSEYAFIYYRLLVEVEYF
                     IALCEIPLPQLADFRKENFTSLRSFYEEFSPDDALKIKEIEKTTNHDVKAVEYFLKDK
                     FIQSGLGRWSEFIHFGLTSQDINNTALPLSLKDALTDAYFPLLFELRETLSGLADEWN
                     DVPMLARTHGQPASPTRLGKELAVFISRLDEQSKYFSQIPYSAKFGGATGNLNAHKAA
                     YPEIDWVSFADSFVNETLGLHRSSPTTQIEHYDNLAALFDNLRRINVILIDLARDFWT
                     YISMDYFRQKTRKGEIGSSAMPHKINPVDFENCEGNLGYANAIFSHMSEKLPVSRLQR
                     DLTDSTVLRNTGVPIGHTIIAFSSLMKGLNRIIINREKIHSDLENNWAVVAEAVQTIL
                     RREGFNKPYEALLELTMTNQKITRGVLESFIDSLEISDDLKSELKAITPFNYTGF"
     gene            3819..5639
                     /locus_tag="PK288_07555"
     CDS             3819..5639
                     /locus_tag="PK288_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM18818.1"
                     /translation="MNKLRLLLKYLEPYKWSAFRNILYNILSALFALFSYTMVIPFLK
                     IMFNRMEPAPYPGEFQLNAEYLSAAARYYMTSLIDKHGETTALLLVCLVVVVASLFKN
                     GFIFLANNSMAYIRSCTVRDLRKKMYDKVLRLPLSYFTEARKGDIMTRISNDVQEIEI
                     SVVSSLTMLFRDPLYIMIFVTYLFVTSTRLTIFALLLLPVSGWLIGRASRTLRSSSFV
                     GQQFLGKLLSAVEETLTGLRIIKGFNAEEKMQKQFSQTNIHYSKVFKRITRKAYLASP
                     ISEFLATIVLMIIMYFGSTLAMKGVGNLDQERLIAFLVVFSQIIQPAKNITTAWFSIQ
                     KGMASVDRIDQVLEAPEKIAEKADALPIRDFKESIEFRGVWFAYNNEPVLKDINLKIL
                     KGQTVAIVGKSGAGKSTLADLLPRFMDVDKGAILIDGIDIRDLKIRDLRNLMGIVSQQ
                     PILFNTSFRENIAFGVDTHSQEQVEYSAHIANAHEFITDTEDGYESTVGESGNKLSGG
                     QRQRISIARAIMANPPILILDEATSALDTESERLVQEAIVNLMKNRTSVVIAHRLTTI
                     RHADLIVVIADGKIVETGTHDELIGRKDGYYNKLHCFQTP"
     gene            5838..7604
                     /locus_tag="PK288_07560"
     CDS             5838..7604
                     /locus_tag="PK288_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPM18819.1"
                     /translation="MIAQKARARHTFSMESSGFMLDGIPFQIISGEMHPARIPAEYWR
                     HRIQMAKAMGCNTIGIYIFWNFHEPREGEYDFTTSNHNLAQFIKTVQDEGMWLLVRPG
                     PYVCAEWDLGGIPPYLLRIPDIKLRCMDPRYMAAAEKYITRLAAELKPWLITNGGPIL
                     MLQIENEYGSYGNDRNYLLRLKEIWQQNGIDVPFFTGDGATPHMLEAGSLPGCAVGLD
                     PGSSPAHFELAEKMNPGVPVFSSETYPGWLTHWGENWARPDTSAFLKEIRFLMDNKKS
                     FNLYVVHGGTNFGFTAGANSGGKGYEPDVTSYDYDAPIDEQGRPTAKYMALRKLIGSY
                     LPKGKKLPDIPAAVEAVEILPIPLNVFTSVWDNLSQPVNSVQPGPFESYNQDYGFILY
                     KTELIGHKKGRLTVTDLHDYATVFVDGEYIGSLDRRLGINSLEIPESKSANPVLEILV
                     EGMGRINFAEHLIDRKGITERVTLNGMTLMNWKIYNLPMSDDYIYNLRSSARNPGKRG
                     VFFKANFFIDKAGDTFFDVSGLKKGIVWVNGHNLGRYWEIGPQKRLYCPGIWLRKGAN
                     EMVVFDLLQLTAPVVTGKKTME"
     gene            complement(7851..8798)
                     /locus_tag="PK288_07565"
     CDS             complement(7851..8798)
                     /locus_tag="PK288_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPM18820.1"
                     /translation="MKNSRSRREFLRLSAAGALGALALSQYSCRNSPGTAGKASAAKS
                     DPKSFGIGLQLYTIRDAMATDVRGSLKQVSDAGYKYVELANYADGKFYGYEPAEFLKI
                     VNDLGMEILSSHTQVEAQGVTLDNAKKMAEDHARLGVKYCIQPWVVEEARKTIDSYRK
                     MVADWNIVGGIMKEHGIMFGYHNHNFEFDTVEGKIPYFDVMLAELDKDLVTMEIDIFW
                     ATKAGQDPVEIFRKYPGRFQLFHMKDMFTKEAPFYTTDGVNDFAPVGAGVIDFRSILA
                     AKDVAGMKYMIVEQDRSREGKPFDDIRTSIANLTTKILV"
     gene            complement(8955..10208)
                     /locus_tag="PK288_07570"
     CDS             complement(8955..10208)
                     /locus_tag="PK288_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HPM18821.1"
                     /translation="MKKIGKYSFGIGDRFGRQGQAQLKAIIDAEKKGCEITPVWNKSN
                     REHIIIGTQPDDVRQEADRTTRNAGFGRPYFVDADHINLDNVERFIESSDFFTIDVAS
                     YIGKRAADSEIERFLSGVARYSRGIKIPGIPRIRKCSNEMLVEIAEKYLFAAKKAGEV
                     YRKIESAKGSGNFITEISMDEVAQPQSPVEILFILKMLSDERIPVQTLAPKFSGRFNK
                     GVDYVGDIKKFAGEFEQDLLVLSYAAGEFGLPENIKISVHSGSDKFSIYPVIGSIIRR
                     HDKGIHVKTAGTTWLEEVIGLAESGNEALEFVKEIYAEAVERIDELCGPYADVIDIDK
                     SKLPSAARVSGWNSRKFAASLRHDSKNPDYNQGMRQLIHIGYKLAAQRSEEFFRLLDE
                     NEHIVEKGVYQNIFTRHLCPLFCIQ"
     gene            complement(10257..11555)
                     /locus_tag="PK288_07575"
     CDS             complement(10257..11555)
                     /locus_tag="PK288_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM18822.1"
                     /translation="MPQNTKTGNYRWTVCALIFFATTVNYLDRQVIGILKPLLESDLN
                     IGEAEYGYIVTAFQIAYALGMVIAGRLIDRFGTKIGYGLSVLLWSIAAMGHALAKGGL
                     GFGFWRAFLGISESGNFPAAIKTVAEWFPKRERALATGIFNSGTNVGAIIAPLAVPAI
                     ATALGWQAAFIITGAIGLLWLILWFIYYEIPEKQKRLSQGELEFIQSDIEEKDEPAKS
                     IPWVQLLRYRQTWMFFIGKGLTDPIWWFYLFWIPGWLASVRGTGLDIKAFGLPLVVIY
                     TSTTIGSIFGGWLSSFMIKKGMSPFKARKIAMLVFALLVTPIVFAQSPNLSTWGAVGL
                     IALAASSHQAWSANIFTTVSDAFPKSAVSSVTGIGGMAGALGGAFVSILAGNLLEYYK
                     NLGHVETGYVIMFAIAGSAYLVAWIIMQVFAPKNKKVTLE"
     gene            complement(11581..>12490)
                     /gene="gnd"
                     /locus_tag="PK288_07580"
     CDS             complement(11581..>12490)
                     /gene="gnd"
                     /locus_tag="PK288_07580"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HPM18823.1"
                     /translation="FVKMVHNGIEYGDMQLICEAYQIMRDMLHLSADEMHSIFREWNE
                     GELNSYLIEITRDILAYKDEDGEPLVDKILDTAGQKGTGKWTGVAALDLGIPLTLIGE
                     AVFSRCLSAIKDERVMASSLLTGPRPGFSGNVAGFIENLREALYASKIVSYAQGYSLM
                     RAAAEEYGWKLNYGGIALMWRGGCIIRSVFLGKIKEAFDNDPGITNLLLDPFFRDKID
                     RAQNGWRAVVAAATAGGIPVPAIASALCYFDGYRCERLPANLLQAQRDYFGAHTYERI
                     DRPRGEFFHTNWTGRGGTTASSTYNV"
BASE COUNT         3222 a         3031 c         2876 g         3361 t
ORIGIN      
        1 tgctgttctc ccaccaccat cttccggaac ggaaatcacc attttcctgg acggccctgg
       61 tgcacggctc acagcctata cttacaaagc ccttgtcgtg caggctgtta tacggcacat
      121 tgtttaattt aacgtattcc cttacctgat tgtatgtcca gttgaacaac gggtaaaatt
      181 tgtaaaggtc cctgtttacg tcatgttcaa cctggtgcat cttactgcgg ctatcggact
      241 ggtcggccct gattccggaa atccagcatg atttgccggc cagcgccctg ttaaggggaa
      301 taaccttcct gatgtgacag cactcgagtc tgttctcctt tgaataatag aaactgtacg
      361 ggcccttttc tgtaaccatt ttttcaacat cttccctgtc ggggaaaaag actttgatgg
      421 gcttattgta ctttttgcgg gtctcgttaa acaccttgta agtttcgggg aaaagcctgc
      481 cggtatcgag cgtcaccact tcaatgtcaa ttgaatttga gaaaatgata tggctgattg
      541 cctggtcctc cagtccgaag ctggttgtaa atatcacttt ccccggaaac aatgatgcag
      601 ctttttccag ccgggcctca acagatcctt ctgataaaag ttcagtcagc tctttcaaat
      661 tcatatcctc tcctcttttt aaggttgatc ttcagcctta ttttatgaat atgagcccag
      721 atacgttcat ggaagtaata aaggatggtt ttggtgagca cctcgatact gccgatggag
      781 acagcaagtg ttaccttccc ggttattatg taacttatta ttattgtgtc aagggtccct
      841 acaatacgcc atgagaccga cttcataaaa ctcttgatgg gcttgtcggc cctttcgcta
      901 accctgatga tccctttttg gtcgtcagtt actgccgaat gattgataag aacatccaac
      961 atagcccttc atttttgatc tgcatcaaaa ataaggcagc cggcggaatg aaacactcgt
     1021 actaatacgg gaaaataatg ggtataaaaa catatagcgc aatgcgcaat cagacctatt
     1081 atccctagaa tgttcaatga ctgttcagct gattccctca ggttcgggat catgcaggaa
     1141 aactcaaccg gaaaataaaa aaagcaaaag acaatgcctt ttgcttcaga acaggcatcc
     1201 tgtcatgata atcactttcc tatcattgtt tttgccagat cgagaatggt ggtgtcgtca
     1261 aaactcacta tgcctcctcc cggtccctgt tcaaaatgca caccaacagt gtctcctttt
     1321 tcaaaatttg tccctccgaa gtagttccgt acagtttctt cgttcagttg aagtttttct
     1381 gcaattttct tcatactacc ggagggtaac tgatctttta gcttccggag ttcattgaat
     1441 gtcattgttt ttgccatttc ctgatattta tgtttttaaa tttgattgta ttcagtctgt
     1501 aaatcaaagt tactcattat atcattaaca taaaaaaata aagtctccaa tcatacaact
     1561 ggtttccggt gaattatcac aaatctttca gcaatttgag gaacaggttg atagcttctc
     1621 ttttgtcatc gttcagatcg aagctcatgt tccgggtcag gtagacataa agatcgttgc
     1681 cgcgtattga tccattagtg ccatattttt ccaccacggc aggaatgtta tccacccctg
     1741 ccttcagggc ggtgttgaaa tccttcagaa aactgtcgtc cagtttcctg ttcgatgtcc
     1801 agcaggcaaa tgcaaaaggc aggccggtat aattgaacca ttctccggcc aggtcagtac
     1861 cgaacctgaa aagcttctca tattcgaagc acagatcgcc gataaggaca acagcctcac
     1921 cgtaaggaat gctgagaaaa tcaaaccctt cactggtatc gcgccaggta aattcctttt
     1981 tccagacatt ttttgcaagg atcctggcca ggcttactga tgacctggac cggtaatcga
     2041 gattgataat gctgatgtct tcaaacggag tgttgctcag gagcattact gttctcacct
     2101 tgccgtacgc tccgagacaa tagtcggtta tcaggtgata ttcatcgagc atgggaagtg
     2161 cagcgaccgg gataagtccg atatctgcac ttccgtttgc cagctttgcc gcacattcgg
     2221 cagggtgatc agttgtaaga atgacttttt tatcgaatcc gctctgggtg aggccatata
     2281 tgaagggata ggtatttgca aaactgacgg ctgaaattct tattttttcg cggttcatac
     2341 ctttgttggt ttacggacta ttctgaatgc aagattccgt ttttttacaa cggctaaaat
     2401 actacttttg tgtataggat tattatctgc ccggaatcaa atcttaacag gatttaccat
     2461 ggaacttaac agcctcacgg caatctcccc tcttgacgga cgataccggc cgaaagttga
     2521 agaactggat gcatattttt cagagtatgc ctttatctat tacaggctgc tggttgaggt
     2581 ggagtatttc attgcgttgt gtgagattcc acttccccag ctcgctgatt tcagaaagga
     2641 gaacttcacc agcctgagat ctttctatga agagttttca cctgatgatg ccctgaaaat
     2701 aaaggagatt gaaaaaacaa cgaaccacga tgtaaaggct gtggaatact tcctcaagga
     2761 taagttcatt cagtcgggtc tgggcagatg gtcggaattc atccatttcg gacttacctc
     2821 acaggatata aacaacaccg cactgccgct ttcgctgaaa gatgcgctaa ctgatgcgta
     2881 ttttccgctt cttttcgaac tcagggaaac cctttcggga ctggcagacg aatggaatga
     2941 tgtacccatg ctggccagaa cgcacgggca gccggcttca ccaacccggc ttggaaagga
     3001 actggctgta tttatctcaa ggctcgacga gcagtcaaaa tatttcagcc agatacctta
     3061 ctccgccaag ttcggtgggg caacaggcaa cctgaacgca cacaaggccg cctatccaga
     3121 aatcgactgg gtttcctttg ccgatagctt tgtgaatgaa accctcggcc tccatcgctc
     3181 aagtcccacc acgcaaattg aacattacga caacctggca gcactttttg acaacctcag
     3241 gagaatcaac gtcatactta ttgatcttgc aagggatttc tggacataca tttcgatgga
     3301 ttatttcagg cagaaaacga gaaagggcga gatagggtcc tctgcaatgc cgcacaagat
     3361 caatcctgta gattttgaaa attgcgaggg caatctcgga tatgccaacg ccattttcag
     3421 ccatatgtcc gaaaagctgc cggtgtcgcg ccttcagcgc gatcttaccg actccactgt
     3481 gctgcggaat acaggcgtac cgattggaca tacgattatt gcattcagtt cattgatgaa
     3541 gggactcaac aggataataa taaatcgcga aaagatccat tccgacctgg aaaacaactg
     3601 ggcagtggtg gcggaagcag ttcagacaat tctgagaagg gagggtttta acaaaccata
     3661 tgaggctctt cttgagctta ccatgacaaa tcagaaaatt acacgcgggg tgcttgaatc
     3721 ttttatagac agtctcgaga tctccgatga tttaaaatcc gagctgaaag cgatcactcc
     3781 ttttaattac actggatttt aaatttgaag tgtaccggat gaataagctc agactcctgc
     3841 tgaaatatct tgaaccttac aaatggtcgg ctttcaggaa tattctatat aatatcctga
     3901 gcgccctgtt tgcacttttc tcatatacga tggtaattcc attcctgaaa ataatgttca
     3961 acaggatgga accagccccg tacccgggtg aatttcagct taatgccgaa tacctgtctg
     4021 ctgcagccag gtattatatg acttcactga tcgacaaaca cggcgagact acggcacttc
     4081 tccttgtctg ccttgtggtt gtggtggcca gcctgttcaa gaacggattt atttttcttg
     4141 cgaacaacag catggcttac atcaggtcat gcaccgtgcg cgacctgcgg aaaaagatgt
     4201 atgataaggt gctgagactt cccctttcat attttaccga ggccaggaaa ggggacataa
     4261 tgaccaggat atcgaacgac gtccaggaaa tcgaaatctc tgtagtgtca tccctaacga
     4321 tgctattcag ggaccccctg tacataatga tctttgtaac ttacctgttt gtaaccagca
     4381 caaggctgac gatttttgcc ctgctgcttc tgcctgtgag cggatggctc atcggacggg
     4441 cgagccgcac actccggtcc tcatcatttg tgggacagca gttcctgggg aaactccttt
     4501 cggcggtgga ggaaacatta acaggattaa ggataataaa gggattcaat gcggaggaga
     4561 aaatgcagaa gcagttttca cagacaaaca ttcattactc caaggtattc aagaggataa
     4621 cccgcaaggc ctatcttgcc agcccgataa gtgaattcct tgcaaccata gtgctgatga
     4681 taatcatgta tttcggaagc acgctggcaa tgaagggagt cggcaacctc gaccaggaaa
     4741 ggctcattgc ttttcttgtc gtattttccc agatcatcca gccggcgaaa aatatcacca
     4801 ccgcatggtt cagtatacag aaaggcatgg cttcggttga caggattgat caggtactgg
     4861 aggcacctga aaagatcgcc gaaaaggccg atgcccttcc catacgcgat ttcaaggaat
     4921 cgatcgaatt caggggagta tggtttgcat acaacaacga accggtactt aaagacatca
     4981 atcttaaaat actcaaaggg cagacggttg ctatcgtggg aaaatcgggc gccggcaaat
     5041 caaccctcgc cgatcttctt ccaaggttca tggatgtaga caagggtgcg atacttatcg
     5101 atggcataga tatacgcgat ctgaagatca gggatttacg aaacctgatg ggaatagtga
     5161 gccagcagcc gatacttttc aatacttcct tccgggagaa tattgctttc ggagttgaca
     5221 cccattcaca ggaacaggtt gaatattcag cgcatattgc gaatgctcat gaatttatta
     5281 cggatacaga ggacgggtat gagagcacgg tgggggaatc gggaaacaaa ttaagcgggg
     5341 gtcagcgtca gcggatcagt attgccaggg cgatcatggc caatcctccc attcttattc
     5401 tcgatgaagc cacctcagcg cttgatactg aatcggaaag gctcgtgcag gaagctattg
     5461 tgaacctgat gaaaaacagg acatccgtcg tcattgccca caggcttacc actatcaggc
     5521 atgctgatct cattgttgta atagctgacg gaaagatagt ggagaccggc actcacgatg
     5581 aacttattgg caggaaggac ggatactaca acaaacttca ttgcttccag accccctgac
     5641 agcaaaggac ggtataatcc atacccttat gaataaactg ctgacggaga ttctggataa
     5701 tgacctgtta attgtatatt tgaaaccggg aattatcttt tatcaggaac tttgcattta
     5761 aacaaactat aatattttgc catatgtcta aacgattctg gttaagcttg ataatgatat
     5821 tacttttatg tcctgccatg attgcacaaa aggccagggc caggcatacg ttttccatgg
     5881 aatcctcggg attcatgctc gacggaatcc catttcagat tataagcggg gaaatgcatc
     5941 ctgcccgcat tcccgccgaa tactggcgcc accggatcca gatggccaaa gccatgggat
     6001 gtaacacaat cggtatttat atcttctgga attttcatga accgagggag ggagaatatg
     6061 atttcacgac cagcaaccat aacctggcgc agttcataaa gaccgtccag gatgagggca
     6121 tgtggcttct tgtgcgcccc ggcccctatg tctgcgccga atgggacctt ggcggtattc
     6181 cgccttacct gctgcggata cccgacatta agctgcggtg catggatccg aggtatatgg
     6241 ctgccgctga aaaatatatc acccgtctgg ctgccgaact taagccatgg ctgataacga
     6301 acggaggacc catactcatg ctccagattg agaatgaata cggcagctat ggcaatgaca
     6361 ggaattacct tttgcggtta aaggagatat ggcagcagaa tgggatcgat gtaccgtttt
     6421 tcaccggcga cggagctacc cctcatatgc tggaagccgg ttcattgccc ggctgtgcgg
     6481 ttggcctgga tccgggaagc agcccggctc attttgaact tgcagaaaaa atgaacccgg
     6541 gagtccccgt cttcagcagc gaaacatatc cggggtggct gacacactgg ggagaaaact
     6601 gggccagacc cgacacttcc gcgttcctga aggaaatcag attcctgatg gataataaaa
     6661 aatcattcaa cctgtatgtt gtccacggag gtaccaattt cggctttaca gccggggcca
     6721 attccggggg aaaaggctat gagcctgatg ttacaagcta cgactatgac gctcccattg
     6781 atgaacaggg acgaccaacg gccaaataca tggcattgcg gaaacttatc ggatcatacc
     6841 tgcctaaggg taaaaagctt ccggatattc ctgctgcggt tgaggcagtt gaaatacttc
     6901 caatacctct caacgtgttc acatctgtct gggataatct ttcccagccc gttaactccg
     6961 ttcagccggg tccttttgaa tcatacaacc aggactacgg attcattctt tacaaaaccg
     7021 aactgattgg tcataaaaag ggccggctta ccgttaccga cctgcatgat tatgccacag
     7081 tatttgtcga cggggagtac atcggttcgc tcgacagacg gttagggatc aattccctgg
     7141 aaataccgga aagcaaaagc gcaaatcctg tgctggaaat ccttgttgaa gggatgggca
     7201 ggataaattt tgcagaacac ctcattgaca gaaagggaat tacggaaagg gtgacattaa
     7261 acgggatgac gctgatgaac tggaagattt acaaccttcc catgagtgat gattatatct
     7321 acaatctccg ttcgtcggca agaaatccgg gaaaacgggg cgtgtttttc aaggcaaact
     7381 tctttatcga caaggccggc gatactttct tcgacgtttc cggtcttaag aaaggcattg
     7441 tgtgggtgaa tggacacaat ctcggacgtt actgggaaat agggccgcag aaaagactct
     7501 actgtccggg gatttggctc cggaagggtg caaatgaaat ggtggttttc gatctgctgc
     7561 agctcacggc accggtggta acaggaaaga aaacgatgga ataattgccg gccggaacct
     7621 gatcagcgaa acctgccggt gaatgtcatt ccgatattct gatcaatctg atccggaact
     7681 ttcacaaacg gagccggcat agtcggcgca ttgcgaaccg tgatggcatt acgcggctgg
     7741 ttaacagcct ccgggagaac tcagatattt ccgcagcatc ccccgggata aaaaacgggg
     7801 atcatttgct gaaaatgatc cccggcagtt attgaataag ctattttgag tcagacaagg
     7861 atctttgtgg tgaggttcgc aatgcttgta cggatatcgt caaacggttt gccttcccgg
     7921 ctcctgtctt gttcaacgat catgtatttc attcctgcaa catcctttgc tgccaggatc
     7981 gacctgaaat cgatcactcc ggccccgacc ggtgcaaaat cattcacgcc gtcggttgta
     8041 tagaagggag cctcttttgt aaacatgtct ttcatgtgga acaactggaa ccttcccgga
     8101 tatttcctga atatctctac cggatcctgg cctgccttgg ttgcccagaa aatatctatt
     8161 tccattgtta ccagatcctt gtcgagttcg gcaagcataa cgtcaaaata gggtatcttt
     8221 ccttcgacgg tgtcaaattc aaaattatga ttgtgatacc cgaacattat gccgtgttcc
     8281 ttcattattc cgcctacaat gttccagtct gcaaccattt tcctgtaact gtcgatggtt
     8341 tttctcgctt cctccaccac ccagggctga atgcagtatt ttaccccgag cctggcatga
     8401 tcctccgcca tctttttggc attgtcaagg gttacaccct gagcttcgac ctgggtgtgg
     8461 ctgctgagta tttccattcc cagatcgttg acaatcttca ggaattccgc tggctcatag
     8521 ccgtaaaact tgccatcggc gtagttggcc agttcaacgt atttgtatcc ggcatcggaa
     8581 acctgtttca gcgaaccgcg gacgtcggtt gccatggcat cccggattgt atagagctgg
     8641 agccctatac cgaatgattt cggatctgat ttggcggcac tggccttgcc tgcggtaccc
     8701 ggagagttcc tgcatgaata ctgggaaagg gctaatgcac cgagagcgcc ggctgctgat
     8761 aatctgagga attccctcct cgaacgtgag tttttcattg ttttgccagt tggttaatat
     8821 ttaattcctt ggtttgatct tccaaagtta aaaaataata ttttctataa atgtaaagtc
     8881 atgcttcgga tttttaagga agaggctggt caaaggaaaa cggaacagga agtaaatgtg
     8941 atccgggtgc cggttcactg aatacagaac agggggcaca aatgccttgt aaaaatattc
     9001 tggtaaaccc ctttttccac tatatgttca ttttcgtcaa gcagcctgaa aaattcctct
     9061 gatctctggg cagcaagctt gtatcctata tggatcagct gtctcattcc ctggttataa
     9121 tccgggttct ttgagtcatg cctgagtgat gccgcaaatt tcctgctgtt ccagcctgat
     9181 accctggcag ctgagggaag ctttgacttg tcaatgtcaa tgacatcggc ataaggaccg
     9241 cataattcat caatcctttc aactgcctca gcgtagatct ccttgacgaa ttcgagggcc
     9301 tcgttcccgg attcagcaag accgatcacc tcttcaagcc aggttgttcc tgcggtcttt
     9361 acatgaatac ccttgtcgtg cctgcggata attgatccga taaccgggta gattgaaaac
     9421 ttatcggacc cggaatgaac gctgatcttg atattctcag gcaggccgaa ttcacctgcg
     9481 gcatagctca gtaccaggag atcctgttca aattcccctg caaatttttt gatatcgccg
     9541 acgtaatcaa cgcctttgtt gaagcgccct gaaaatttcg gcgccagtgt ctgaaccggg
     9601 atcctttcat cacttaacat cttgagtata aagagtattt caacgggaga ctggggctgg
     9661 gccacctcat ccatcgagat ttcagtaatg aaattccccg acccttttgc agattcaatt
     9721 ttcctgtaca cctcgcctgc cttttttgca gcaaagaggt atttttcggc aatttccacg
     9781 agcatttcat tggagcattt gcggatcctg ggaatccccg ggatctttat tccccttgag
     9841 taccgcgcca ctccggaaag gaatctttcg atctcgctgt ctgcggcccg cttgcctata
     9901 tatgaagcca catcaatggt gaaaaaatca gaactttcta tgaatctctc cacattgtcc
     9961 aggttaatgt gatcggcatc gacaaaatat ggtctgccaa agcctgcatt cctggttgtt
    10021 ctgtcagctt cctgccttac atcatcgggc tgggttccaa taatgatatg ctccctgttc
    10081 gatttgttcc agacaggtgt gatttcacag cctttttttt cagcatcaat tattgctttg
    10141 agctgggcct gcccctgtcg tccgaacctg tctccgatcc cgaaggagta ctttccaatt
    10201 tttttcatct gaacgataat aaattaaacc taagcggaca ttgtttcccg gagaagtcac
    10261 tcaagtgtta ccttcttgtt tttgggtgca aatacctgca tgattatcca ggccaccagg
    10321 tatgcgctcc ccgcaattgc gaacattatt acatatcctg tttccacgtg tcccagattt
    10381 ttatagtatt ccagcaggtt gccggccagg atggaaacga atgcaccgcc cagggcgccc
    10441 gccattcctc cgatacccgt caccgagctg actgcgcttt tggggaaagc atcagatacg
    10501 gtggtgaata tgtttgccga ccatgcctgg tgcgatgaag ctgccagggc gatgagccca
    10561 actgctcccc aggtactgag attcggtgat tgcgcaaaaa ctatgggtgt gaccagcagg
    10621 gcaaagacca gcattgcaat tttcctggct ttgaatggtg acattccctt ttttatcatg
    10681 aatgatgaga gccagccccc gaaaatgctt ccgatggtgg tggaggtata aataaccacc
    10741 agcggcaggc cgaaggcttt tatatcaagc cccgtgcctc tgacggatgc aagccatccg
    10801 ggtatccaga acaggtaaaa ccaccagatc ggatcagtaa gaccctttcc gataaagaac
    10861 atccatgtct gcctgtatct gagaagctga acccacggga tgctttttgc aggttcatcc
    10921 ttttcctcaa tgtcactctg gataaattca agttctccct gcgaaagcct tttttgcttc
    10981 tcgggaattt catagtagat aaaccagaga atgagccaca gtaacccaat agccccggtt
    11041 attatgaacg ctgcctgcca tccgagggcg gtggcaatag cagggacagc aagcggtgca
    11101 attattgctc ccacattggt tccggaattg aaaataccgg tggcaagggc cctttccctc
    11161 ttgggaaacc attctgcaac agtttttatc gcagccggaa aattacccga ctcactgatc
    11221 ccgagaaacg ccctccagaa tccaaaaccc agtccgcctt tggccagggc atgtcccatg
    11281 gcggcgatgc tccacaggag aaccgataat ccatagccta ttttcgtgcc gaacctgtca
    11341 atcaggcgtc cggcaatcac cattcccagt gcataggcta tctggaaggc tgtgacaata
    11401 tatccgtatt cagcttctcc aatattgagg tcggattcaa gcaggggttt cagtatacct
    11461 attacctgcc tgtcgagata gttaacggtg gttgcaaaaa atatcagggc acatacggtc
    11521 cacctgtagt ttccggtttt ggtattttgc ggcattgtgt ttcagtttga gtttcaggga
    11581 ttatacgtta taggttgatg aagcggtggt tcctccgcgc ccggtccagt tcgtatgaaa
    11641 gaattcgcct cttggtctgt cgatcctttc ataggtatgc gcgccaaagt aatcgcgctg
    11701 ggcctggagc agatttgccg gcagcctttc acatctgtat ccgtcaaagt aacaaagggc
    11761 agatgctatg gccggaacag ggataccgcc ggcagttgcg gcagccacaa ctgccctcca
    11821 tccattctgt gccctgtcga tcttatccct gaagaacggg tcgagcagca ggttggttat
    11881 tccgggatcg ttgtcaaaag cctctttaat cttgcccagg aaaaccgacc ggataataca
    11941 gccgcctctc cacataaggg ctattccccc gtaatttagc ttccagccgt attcctctgc
    12001 cgcagctctc atcagtgagt atccctgtgc atacgacact atcttcgatg cgtacaatgc
    12061 ttcgcggagg ttttcaataa atcctgcgac gttgccggaa aagcccggac gcggtccggt
    12121 cagcagggag gatgccataa cccgctcatc ctttatcgcg gacaggcatc tcgaaaaaac
    12181 tgcttctcct atcagtgtga gtggtattcc caggtcgagg gctgccaccc cggtccattt
    12241 acccgttccc ttctgccctg cagtatccag gatcttgtca accaggggtt ctccgtcttc
    12301 atccttgtat gccagaatat ccctggtgat ctcgatcagg taactgttga gctcaccctc
    12361 gttccattcc ctgaaaatgg aatgcatctc atcagcactc aggtgaagca tgtccctcat
    12421 gatctgatag gcttcgcaga taagctgcat atcgccgtac tcaattccgt tatgaaccat
    12481 tttgacaaaa
//
LOCUS       DAOOYG010000076        12197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_5433_length_12197_cov_8.342365, whole genome shotgun sequence.
ACCESSION   DAOOYG010000076 DAOOYG010000000
VERSION     DAOOYG010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12197
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5433_length_12197_cov_8.342365"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PK288_07585"
     CDS             <1..551
                     /locus_tag="PK288_07585"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPM18824.1"
                     /translation="LEIRPVIKTEFNIEGYPSDKFIELVLSVKPDQVTLVPDAHDAIT
                     SNAGWNVPRFRLFLADVVSVFRKEGIRTSIFVDTDPVNIETAAMTGADRVELYTEPYA
                     AGFDKDAPSAIAPFINAAKIARDSGLGLNAGHDLNLDNLRYLHQNIPWIDEVSIGHAL
                     ISDALYLGLENTIQMYRRQLAG"
     gene            560..1366
                     /locus_tag="PK288_07590"
     CDS             560..1366
                     /locus_tag="PK288_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPM18825.1"
                     /translation="MMLFYRKYGSGPALMILHGLYGSSDNWVSIAGKIDDRFTVIIPD
                     LRNHGASPHSSIHDYDAMSLDIKELADELNLGHFFLAGHSMGGKAAMKFAMRWPERLL
                     GLLIADISPVNSTERFKKDLEKHREIMQAMISADISSASSRKEAEALFSSLQSEKLTG
                     FILKNLRRKTDGGFTWKINLGTLAASIENIMASVEPGKNEDRSISGFPVFFLKAGKSD
                     YITDDDIGPILKIFPGAEIITIPEAGHWIHSDNPEAVVSALLKFLETDQD"
     gene            complement(1370..2089)
                     /locus_tag="PK288_07595"
     CDS             complement(1370..2089)
                     /locus_tag="PK288_07595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPM18826.1"
                     /translation="MLVNIKPEDILFLDIETVPSASSYYDLDPAFRIHWEKKSKQFRN
                     DDQLPGQVYNRAGIFAEFGKIICISAGFISAKDPFSFRIKSFYGDNEKDILTGFSELV
                     NKFSRNREMNLCAHNGREFDYPYIARRMIINRLAIPGILDNAGKKPWEVRLLDTLDLW
                     KFGDYKSYTSLDLLTLVLGINSPKDDIDGSMVAGIYHDEHDLKRIVVYCEKDVAAVAQ
                     VLLRFMNLPSIDPGRIESVTF"
     gene            complement(2106..2714)
                     /gene="udk"
                     /locus_tag="PK288_07600"
     CDS             complement(2106..2714)
                     /gene="udk"
                     /locus_tag="PK288_07600"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPM18827.1"
                     /translation="MLIVGIAGGTGSGKTTVVKKVMERFPSGEVIVLPQDSYYRDNRH
                     IPPEERQKINFDHPDSVEFSLLMEHIERLRKGQSIEMPVYSYLTCLRSKETLTVDPAR
                     VALVEGILILADPGLRDVLDIKVFVDADPDDRLGRVIKRDIEDRGRSVLQVLERYHDT
                     VKPSHLQFIEPSKRYADIIIPGGGENQIGIEVLISIIEKHLY"
     gene            2877..3872
                     /locus_tag="PK288_07605"
     CDS             2877..3872
                     /locus_tag="PK288_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPM18828.1"
                     /translation="MSELYPLKFEPILKEKIWGGQLLANHYGKNPVGLNNIGESWELS
                     SLRGDLSVVSNGFLAGNNIEEIIEVYMGDIVGDAVFEKFGIEFPLLIKLIEAEQDLSV
                     QVHPGDEQAKRLHNSFGKTEMWYILESSNNGGIYTGFREPLNRERYLEALGNNTIPDL
                     MNFEIAEPGDTFFTPAGRIHAIGAGVVLAEIQQASDITYRIYDWGRTNEKGEPRELHT
                     ELALEAIDYGAAGKIKLKKPIAENRTSVLVSNEFFNTNILHFNQSVSKDYTPIDSFVV
                     YLCTEGRFNIRYNNSTEIVTKGETVLLPAMIKNVILDPGPEATLLEIYIRINQTN"
     gene            3891..4661
                     /locus_tag="PK288_07610"
     CDS             3891..4661
                     /locus_tag="PK288_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18829.1"
                     /translation="MNIFYKQVFVIFIALVFAGCSGKGSAKKESVAEADTMQIPDTGF
                     TGIKKYYSINRLIKEVTFKNGIRHGEMKSFYQGGQLYQTFWYENGLKEDSAKWYYLEG
                     PVFRSTPFLHDTIHGTQIQYYRNGRVKAKLHFIKGLRTPELEEFTRDGKRIGNYPDIS
                     YRLTDNYAAAGRLRINLELTNQAIKTKFYRGEFYNGVFDTARVEPIKTTNGKAVLDLK
                     KSNKPQRNYIGIIAEIITDFGNKLFVYKRIELPYGDLN"
     gene            4673..5281
                     /gene="yihA"
                     /locus_tag="PK288_07615"
     CDS             4673..5281
                     /gene="yihA"
                     /locus_tag="PK288_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPM18830.1"
                     /translation="MIIRSAAFVSSSTSVRHCPPADKPEFAFIGRSNVGKSSIINMLT
                     GWSRLARTSSDPGKTRTINHFLINGSWYLVDLPGYGYAKVSRSLRNEWMKSVEEYILK
                     RENLACLFVLIDSRHEPQRQDLDFMEFLGTNELPFARIFTKTDKTSTASVRKTLKLYD
                     TVMLEDWEALPPTFISSAVKKEGRKEILDFIEETINKFSNSG"
     gene            5350..6288
                     /locus_tag="PK288_07620"
     CDS             5350..6288
                     /locus_tag="PK288_07620"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPM18831.1"
                     /translation="MYGKAPMKVFSCRSSRHLAQKIADHLGISLGQSSVTEFSDGEFQ
                     PCFDESVRGNMVFIVQSTNPPADNLFELLLMIDAAKRASAYKVIAVIPYFGFARQDRK
                     DRPRVSIGSKLAADLLGTAGVDRVITMDLHADQIQGFFNVPVDHLFGSAIFAPYIENL
                     HLDNLTVSAPDMGGSKRANAYSRHLHSEMVLCYKLRKKPNVVEEMSIIGEVEGKNVVI
                     IDDMVDTARTLVLAAQKMEEKKARSIRAFATHPVLSGNAVERINDSPISELVVTDSVP
                     LPRQSDKIKVLSIAEIFARTIQAVNLNQSISTSFVF"
     gene            6388..7005
                     /locus_tag="PK288_07625"
     CDS             6388..7005
                     /locus_tag="PK288_07625"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPM18832.1"
                     /translation="MKTIEIRGTVRTGLGKKNSKQTRKEGNVPCVIYGRDQNIHFHAH
                     ENSFKNLVYTPNAYIVNLDLDGSKHELIMQDIQFHPVTDRIIHIDFLEIDEKKPIVIS
                     LPIKVVGDSIGVKAGGKLRIKKRSLKVRGLASNIPDFLPIDITNLKIHQSIKIGDLSY
                     EKIELTEPKITTVLTVATSRVIQKSEGEGEPAAAAAEPSEPAAAE"
     gene            7051..7662
                     /gene="pth"
                     /locus_tag="PK288_07630"
     CDS             7051..7662
                     /gene="pth"
                     /locus_tag="PK288_07630"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPM18833.1"
                     /translation="MKYLIVGLGNTGEEYEDTRHNIGFTILDAFADASNVVFTDKRYG
                     SVCNVRLKGRELILLKPSTYMNLSGYAVDYWMKREKIPGENLLVIVDDIALPHGSFRM
                     RPKGGAGGHNGLLHINAILGTTEYARIRIGIGNGFKKGSQINFVLGKWSSEEKLFLQK
                     RIPVIIEMIKSFVLSGTELTMTAFNKLGRPAQEKEDTPRQENS"
     gene            7694..8089
                     /locus_tag="PK288_07635"
     CDS             7694..8089
                     /locus_tag="PK288_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPM18834.1"
                     /translation="MTEGKPVRIDRFLWAVRIYKTRSIAAEECRKGRIIIRGIQVKPS
                     KPVMKNDIIIVKKLPVTYSYRVIEPVEKRVSANLVSAHIEDLTTEDEKMKLEIKKSDI
                     NGFRLKGAGRPTKKERRNLDRLRMNLNNS"
     gene            8242..9570
                     /locus_tag="PK288_07640"
     CDS             8242..9570
                     /locus_tag="PK288_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM18835.1"
                     /translation="MGRNNIEKFSGRYLVLKKLIGFWHNKIYYRKVIVIGRENINPDD
                     HLIYAPNHQNALMDALAVLFTHKGQPVFLARADIFKRRIIASILYFMKILPVYRIRDG
                     FSSLKSNDEIFHKTIDVIKNKNGLVILPEGDHAGFRRLRQLKKGICRIAFQADEATGF
                     NLKIKIIPVGLDFTSYTRFRQVLTVVYGKPIEVSEFHQLYRENPERALTELRNRLSAE
                     MKRLIVHIESVEDYEAIDELRAIINGKYSDDIQNPKLFRDRQLIDKLNNLKVSDLMKY
                     NRICSLSLEIKNKTDALNLTYRLLEKRKNPLGWLIAGAAGLAATFPLWLFGNIFTLTF
                     LTIPDTQIRKIKDLQFHSSIRFGISFVLALILFPIYLVICLLLFSPWWLALIIFLGIP
                     LTGLFAWNYNLIFRRILGGFRVRKLKKHNHKDYLLLKNKFDDLITLVAEL"
     gene            9572..10378
                     /locus_tag="PK288_07645"
     CDS             9572..10378
                     /locus_tag="PK288_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM18836.1"
                     /translation="MRDNKLFEGKVVWITGASSGIGEALVYNFASLGSELIISSNDPA
                     GLERVKENCGMKSEKICCVPFDLSATEDIREITEKQINRTGKIDYLLNIGGVSQRCRI
                     EETPLWLDRKIFEINYFGTIALTKAVLPFMIRQKSGHIIATSSISGRFGFPLRSAYSA
                     SKQAIHGFIETLYLENKANNIRASVIIPGRVRTNISLHALNSDGREHGVMDPGQEGGI
                     PAEKAAAIIIKGIIRNKREILVGGKELLLLHVRRTAPWLFFRIADKIDTM"
     gene            10402..11469
                     /locus_tag="PK288_07650"
     CDS             10402..11469
                     /locus_tag="PK288_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPM18837.1"
                     /translation="MEYVIGGIQQIGIGVMNIEESWKWYIDMFGMDCKIFDDDSEANL
                     MSRYTGGTPVKRRAVLAYNLQSGSGFEVWQYKGRKPAEKSTMIKLGDLGILICKIKTR
                     NLKKTFEYFGRKNVNILGKPVRDPAGSESFFMKDPYNNLFQVMEFNHWFIDEKKPTGG
                     SCGAIIGVSDIDKARVVYSGILGYDEVVYDSTGIFPDLACLPGGDKKIRRVLLKCSKP
                     FNGPFIKVFGQSVIELVCDPEESGEKTLSGRLWGDPGFIHLCYDISGIDSLMETCNKA
                     GHPFTVDSRSNQNGDSFGMGAAAGHFAYIEDPDGTLIEFVETHKLPVLKKFGWYIDLK
                     KRRTYKPLPDWMIKMLRFSKV"
     gene            complement(11466..>12197)
                     /locus_tag="PK288_07655"
     CDS             complement(11466..>12197)
                     /locus_tag="PK288_07655"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPM18838.1"
                     /translation="YYDLGARYITLCHTRNNDICDSSTDPAGPEHNGLSDLGKQVVRE
                     MNRQGMMVDVSHISDKAFYDVLQISTAPVIASHSCCRALCESPRNLTDDMLLALKANG
                     GVVQICLLSGYVKTPAPNPEFEEKLKELRNTFGEYEKLSDEKKELMFNEYVKIKEKYE
                     KLATVRDVVDHIDHVVQVAGIDFVGIGTDFDGGGSVEGCKSAAEMRNITIELLRRGYS
                     KADISKIWGGNIMRVLRAASLGRKI"
BASE COUNT         3715 a         2704 c         2680 g         3098 t
ORIGIN      
        1 tgcttgagat ccggcccgtg ataaaaaccg aattcaatat cgaaggatat ccttctgata
       61 agtttatcga gctcgtattg tcagtcaaac ccgaccaggt tacacttgtt cccgacgctc
      121 acgatgccat tacttcaaat gccggatgga atgtcccgcg gttcaggctt ttccttgccg
      181 atgtagtctc cgtattcagg aaagaaggta tcagaacatc aatatttgtg gatacggatc
      241 cagtcaacat tgaaaccgcc gccatgaccg gagccgacag ggtcgaactc tacaccgaac
      301 cctacgcagc cggttttgac aaggacgcac cctcagccat agccccgttc atcaatgcag
      361 caaaaatagc ccgcgattca ggcctcgggc tcaacgcggg ccacgacctg aatctcgata
      421 atctccgata cctgcatcag aatatcccct ggatagatga agtatcaatc ggacatgcac
      481 ttatttccga cgcactctat ctgggcctgg agaacaccat ccaaatgtac aggaggcagc
      541 ttgcaggctg aaaacccaca tgatgctttt ttacaggaaa tatggctcag gtcctgccct
      601 gatgatttta cacggcctgt atggatcatc ggataactgg gtatcaatag ccgggaagat
      661 tgatgacaga ttcacggtaa tcattcccga tctcagaaat cacggggcat cccctcattc
      721 ctcaattcat gattatgatg caatgagcct cgacatcaaa gaactggctg atgaactgaa
      781 cctcggtcat ttcttccttg ccggccacag catgggagga aaggctgcca tgaaatttgc
      841 aatgagatgg cctgaaagac tcctcggact gcttatcgca gacatctccc ctgtaaactc
      901 aaccgaacga ttcaaaaagg atctggaaaa acatagggaa atcatgcagg caatgatctc
      961 ggcggatata tcctcagcaa gttcacgaaa agaagctgag gctttattct catcattgca
     1021 atctgaaaaa cttaccggat tcatacttaa aaacctgcga agaaaaaccg atggcggatt
     1081 cacctggaaa ataaaccttg gaaccctggc tgccagcatt gaaaatataa tggcatccgt
     1141 ggaaccggga aaaaatgagg accggagtat cagcggcttt cctgtcttct tccttaaggc
     1201 cggaaaatca gattatataa ccgatgacga tatcggtccc attctgaaaa tattccccgg
     1261 agcagagata ataaccattc ctgaagccgg acactggatc cattcagata accctgaagc
     1321 agttgtatca gcgctgttga aattccttga aaccgatcag gactgattcc taaaatgtga
     1381 ccgattcaat tcgtccggga tcaatcgaag ggagattcat gaatcttaga agcacctggg
     1441 caaccgctgc aacatccttt tcacaataaa caactatgcg tttaagatcg tgctcatcat
     1501 ggtagatccc ggcaaccatg ctgccgtcaa tgtcatcctt gggggagttg atgccaagta
     1561 ccagtgtaag aagatcgagt gatgtataac tcttgtagtc gccgaatttc cagagatcga
     1621 gagtatcaag caacctgact tcccagggtt tcttaccagc attgtcgaga atacctggaa
     1681 tggcaagcct gtttattatc atccgccgtg ctatatacgg atagtcaaat tccctgccgt
     1741 tatgcgcaca aagattcatt tccctgtttc gtgagaattt atttaccagt tccgaaaatc
     1801 ctgtaaggat atccttctca ttatccccat aaaacgattt gatcctgaaa gagaaaggat
     1861 cctttgcact gataaatccg gccgaaatgc agattatttt tccaaattct gcaaatatgc
     1921 ctgccctgtt ataaacctgg cccggcaact ggtcatcgtt cctgaactgt tttgattttt
     1981 tttcccaatg tatcctgaag gccgggtcaa gatcataata tgaggaagct gaaggaactg
     2041 tttcaatgtc aaggaacaga atatcctcgg gcttaatatt cacaagcatc gcagcataat
     2101 attgattagt aaagatgttt ctcaattatg gagatcagaa cctcaatgcc gatctgattt
     2161 tccccgcctc cgggaattat tatgtctgca tatctttttg aaggctcaat gaactggaga
     2221 tgggatggtt taaccgtgtc gtgatatctt tcaagcacct gcagaacgga tcttcccctg
     2281 tcctctatat cccgttttat aacccgtccg aggcgatcat cggggtctgc atccacaaaa
     2341 accttgatat caagcacatc ccgcaatccc ggatcagcca ggataaggat accctcaacc
     2401 aaagcaacgc gtgccggatc aacggttaaa gtctccttcg atctcaggca tgtaagatag
     2461 gagtatacag gcatttcaat gctctgtccc tttctgagcc gttcaatatg ttccatcagc
     2521 aggctgaact caacagaatc gggatgatca aaattgatct tctgacgttc ctccggaggg
     2581 atatggcgat tatccctgta atatgagtcc tggggtaaaa caattacctc tccggatgga
     2641 aatctctcca tcactttctt taccacagtg gtttttcccg atcctgttcc cccggcaata
     2701 ccaactatca gcattaattc ataaatttgc atcagattat cctttaaaaa taataatttt
     2761 taaaagatcg gggccagcga atcatatctg gattgatcct gatcgaagga ttccggaatt
     2821 tataaactat ttacacttta aatcattttt acaatcagct atttaaaaat cagatcatgt
     2881 cggaacttta ccctcttaag tttgagccaa tcctgaagga aaaaatatgg ggcgggcagt
     2941 tacttgcaaa tcattatggc aaaaacccgg ttggattgaa taacattggg gaaagctggg
     3001 agctttcatc gctgaggggt gatctgtcgg tcgtcagcaa tggtttcctg gcaggcaaca
     3061 atattgagga aataatcgag gtttatatgg gagatattgt tggggatgct gtttttgaga
     3121 aatttggtat tgagtttcct ctgctcatca aactcattga agccgaacag gatctgtcgg
     3181 tacaggtcca tccgggcgac gaacaggcaa agcggcttca taattcattc ggaaaaacag
     3241 agatgtggta tattctcgag agttcgaata acgggggtat ttatacaggt ttcagggaac
     3301 ctttgaaccg cgaaagatac cttgaggctc tcgggaacaa tactatcccg gatctgatga
     3361 atttcgagat tgcggaaccg ggtgatactt tttttactcc tgccggaaga atccacgcca
     3421 ttggagccgg agtagtcctg gccgaaatac aacaggcatc ggatataaca tacaggattt
     3481 acgactgggg aagaacgaac gaaaagggtg aaccccggga acttcatacc gaacttgctc
     3541 tcgaggccat cgattacggg gcggccggaa aaatcaaact gaaaaaacca attgcagaaa
     3601 acagaacatc tgttcttgta agcaacgagt tcttcaatac caatatcctt cactttaatc
     3661 agtctgtcag taaagactac actcccatcg attcatttgt tgtttacctt tgcaccgagg
     3721 gaagatttaa catccggtac aacaacagta ccgaaattgt aaccaaaggt gaaacggtcc
     3781 tgcttcctgc catgataaag aatgtcatcc tcgatcccgg acccgaagcc actttgcttg
     3841 aaatatacat ccggattaat cagacaaatt gaacattaac atcagctgca atgaatatat
     3901 tttacaaaca ggttttcgtt atttttatag ccctggtttt tgccgggtgt tccggcaaag
     3961 gttcagcaaa aaaagaatcc gttgctgagg ccgataccat gcagataccg gatacaggct
     4021 ttaccgggat aaaaaaatat tacagcatca accgtcttat caaggaagtt accttcaaga
     4081 acgggatacg tcatggagaa atgaaatcat tttaccaggg cggacagctt taccagactt
     4141 tctggtatga aaacggattg aaggaagatt ctgcaaaatg gtattatctg gaaggtccgg
     4201 ttttcaggtc aaccccgttt cttcatgata caatacacgg aacccagata caatattacc
     4261 gcaacggaag ggtcaaggct aagctgcact tcatcaaagg actccgcaca cccgaactgg
     4321 aagaattcac cagggacgga aagcgtattg gcaactatcc ggatatttcc tacaggctga
     4381 cagataacta cgctgcagcc ggcagattaa gaatcaacct ggaactgaca aaccaggcga
     4441 taaagaccaa attctacagg ggcgaattct ataacggggt ttttgacaca gcaagagttg
     4501 aaccgataaa gacaacaaac gggaaggcag ttctcgacct taaaaaaagt aataaacccc
     4561 aaaggaacta tatcggaatt atagctgaaa ttataaccga tttcggcaac aagctgtttg
     4621 tttacaaaag gatagaatta ccatacgggg atctgaacta ggtaaacaag gtatgattat
     4681 ccgttcggcc gcattcgtaa gcagcagtac atctgtcagg cattgccccc ccgcggacaa
     4741 accagaattt gcatttattg gcagatcaaa tgttgggaaa tcatccatta taaatatgct
     4801 caccggatgg tcgcgactgg cccggacttc ttccgatccg gggaaaacaa gaacaataaa
     4861 ccatttcctg atcaacggaa gctggtacct ggtggacctg cccggctacg ggtatgcaaa
     4921 agtatccagg agcctcagga atgaatggat gaagtccgtt gaagagtaca tactgaaaag
     4981 agagaatctg gcgtgtcttt tcgtacttat cgacagcagg catgaacccc agcggcagga
     5041 tctggatttt atggaattcc tgggaacaaa cgagctgcct tttgcgcgaa tttttacaaa
     5101 aaccgacaaa acatcaactg catcggtgag gaaaacattg aagctttatg atactgtcat
     5161 gctggaagac tgggaagccc tgcctccaac atttatttcg tctgctgtga aaaaagaagg
     5221 caggaaagag atcctggatt ttatcgaaga aactatcaac aagttttcaa attcaggatg
     5281 attctttccg gaatttctat atttgtatcc ttttaatctc ccaattcata acattttaat
     5341 tccagagcaa tgtacggtaa agcccctatg aaagtcttct cctgcagatc ctcaaggcac
     5401 cttgcacaga aaattgcaga tcatctggga atcagcctgg gtcaaagttc cgtgacggaa
     5461 ttcagtgatg gagaatttca accctgtttc gacgaatcgg tcaggggtaa tatggttttc
     5521 atagtgcaat ccacaaaccc tccggcagat aatttgttcg aactgcttct catgattgat
     5581 gctgccaaga gagcctcagc ctataaagta attgccgtga ttccctattt tggttttgcc
     5641 aggcaggaca gaaaagacag gccaagggtt tccattggat caaagctggc tgccgacctt
     5701 ttgggcacag caggtgtcga ccgggttatc acgatggatc tgcatgctga ccagattcag
     5761 gggtttttta acgtacctgt tgaccacctt ttcggatcgg caatctttgc accgtatata
     5821 gaaaatcttc acctcgacaa tcttacagta tctgctcccg atatgggcgg ttcaaaacgg
     5881 gcaaatgcct actccaggca tcttcattct gaaatggttt tgtgctataa actgcgtaaa
     5941 aaacccaacg tggtcgagga aatgtcaatc atcggggaag ttgaagggaa aaatgtggta
     6001 ataatagatg atatggttga tactgcaagg acactggttc tggccgccca gaaaatggag
     6061 gaaaaaaagg ccaggtcgat cagggccttc gccacgcatc ccgttctcag cggaaatgct
     6121 gtggaaagaa ttaacgactc gcccatttct gaactcgtgg tgactgattc agtgccgctt
     6181 ccacgtcaat cggacaaaat aaaggtgctg tcaatagcag agatctttgc aaggacaatc
     6241 caggctgtta acctgaatca atcgatctcc actagttttg tattctgaat cctttctatt
     6301 atatttgcac tccatttttt tgatgtcaaa cgtgtaatgg ggagatgtac aacatacaac
     6361 tctgagtagc acaataacaa ttaccgaatg aaaacaattg aaatcagggg aacggtcagg
     6421 acaggactgg gcaagaaaaa ctcaaaacag accaggaagg aaggaaatgt accttgcgtt
     6481 atatatggaa gggatcaaaa catacatttc catgcccatg agaacagttt taaaaatctt
     6541 gtctatacgc ccaatgccta tattgtaaac ctcgacctgg atggcagcaa acatgagctg
     6601 attatgcagg atatccagtt ccacccggtt accgaccgga ttatccatat tgatttttta
     6661 gaaatagatg aaaaaaaacc catagtaatc agtcttccca ttaaagtcgt tggagattca
     6721 atcggcgtca aagccggcgg taaactcaga ataaagaaaa gaagcctcaa ggtcaggggc
     6781 ctggcaagca atatccctga tttcctgcct attgacataa ccaatctcaa aattcatcaa
     6841 tccataaaaa taggcgacct ctcctatgaa aagatagaac tgaccgaacc taagataaca
     6901 actgtgctta ctgttgccac atcgagggta atccagaaat cagaaggcga gggtgaacct
     6961 gctgctgccg ctgccgaacc gagtgaacct gctgctgccg agtaagcagc cagtgaactc
     7021 ttcggataaa taattcaggc acaatgaaac atgaaatatc ttatagttgg cctgggcaat
     7081 accggtgaag aatacgagga tacccggcat aacataggtt tcacgatatt ggatgcattt
     7141 gcagatgcat ccaatgttgt ttttaccgac aaacgatacg gatcggtgtg caatgttcgc
     7201 cttaaaggac gggagcttat actcctgaaa ccttcaacct acatgaatct gagtggatat
     7261 gccgtagatt actggatgaa aagggaaaag atcccaggcg aaaatcttct cgtaattgtg
     7321 gatgatattg cattgccgca cggcagtttc agaatgaggc ccaagggtgg cgcaggcggt
     7381 cataacggac ttctgcatat aaatgccatt cttggaacaa cagaatacgc aaggatcagg
     7441 atcggaatcg gaaacggttt caaaaaaggc agccagataa atttcgtcct tggcaagtgg
     7501 agcagtgagg aaaagttatt tctgcagaaa agaatcccgg taatcattga aatgataaaa
     7561 tcattcgttt tatccggaac tgagcttaca atgactgctt ttaacaaact gggcaggcct
     7621 gcgcaggaaa aggaagatac tccccggcag gaaaactcat gattaaagcc actatcttaa
     7681 ttagcaagac acaatgacgg aaggaaaacc tgtaagaatt gacagattcc tctgggcggt
     7741 ccgtatctac aagactcgca gcatagccgc cgaagaatgc agaaaaggaa ggataatcat
     7801 caggggtata caggtcaaac cgtcaaaacc tgttatgaag aatgatatca ttatcgttaa
     7861 aaaacttccg gtaacttatt cataccgggt gatagaaccc gtggagaaac gggtttctgc
     7921 aaatcttgta tccgctcata ttgaagatct tacaacggaa gatgaaaaaa tgaagcttga
     7981 gataaaaaaa tcggatatta atggcttcag actcaaaggc gcaggcaggc caacaaaaaa
     8041 ggaaagaaga aatctcgaca ggttgagaat gaatctgaat aacagttaaa ggtggtataa
     8101 agacttatta ttgttgaaaa ccagtctaaa ataaattgcg gcacctcaat ccgggcttca
     8161 ttttttttaa cttgcagcta tctgaatatc tcatcagcat gcacgtaaag gactggagca
     8221 gcatcttaaa aacctgatca aatgggtagg aataatattg agaaattttc cgggagatat
     8281 ttagttctga aaaaactcat tggtttctgg cataacaaga tctattacag gaaagtaatc
     8341 gttatcggaa gggagaatat aaatcctgat gaccacctga tatacgcacc caatcatcag
     8401 aatgccctta tggatgccct ggcggtactt ttcacacaca agggtcagcc tgtcttcctc
     8461 gcaagagccg acatattcaa aagaaggatc attgcatcaa tcctctattt catgaaaatc
     8521 cttcccgttt acaggataag ggacggattc agttctctta agtcaaatga tgaaatcttt
     8581 cataaaacga tagatgttat caaaaataaa aacggactgg taattcttcc cgagggagac
     8641 catgcgggtt tccggaggct ccgccagtta aagaaaggta tctgccggat tgctttccag
     8701 gccgatgagg caactggttt taatcttaag atcaaaatca tacccgttgg gcttgatttt
     8761 accagttata cccggtttcg ccaggtacta accgtagtgt acggaaaacc aattgaagta
     8821 tctgaattcc atcagctcta cagggaaaat cccgaaagag cattgaccga attgagaaac
     8881 aggctgtcag ccgagatgaa aagactgata gtgcacattg aatccgtgga agattacgag
     8941 gcaatagacg aactcagggc aataataaat ggaaaataca gtgatgacat tcagaatcct
     9001 aaattattca gagacaggca gctaattgac aaactcaaca atcttaaagt cagcgatctg
     9061 atgaaataca accggatttg ttccctgagc ctggagatta aaaacaaaac cgatgcattg
     9121 aatctgactt acagattact tgaaaagaga aaaaatccac ttggctggct gatcgccgga
     9181 gcagccggcc tggctgcaac atttccttta tggcttttcg gcaacatttt cactcttaca
     9241 tttcttacca tacctgatac ccagatccgc aaaataaaag atctccagtt ccacagcagc
     9301 attagattcg gaatatcatt tgtactggca cttatcctct ttcccatata ccttgtcata
     9361 tgcctgcttc tgttttcacc atggtggctg gccctgatta tattcctggg tatccccctg
     9421 accggattat ttgcctggaa ttacaatctc attttccgcc ggatcctggg aggattccgg
     9481 gtgcgaaagt taaaaaaaca taatcacaag gattatttgc tgctaaagaa taaattcgac
     9541 gatttaataa cattggttgc cgagctgtga gatgagagat aataaattgt ttgaagggaa
     9601 agtggtctgg atcaccggtg catcctccgg cattggagag gcacttgtat acaattttgc
     9661 atcgctggga tcagaactaa taatttcctc caacgatccg gccggattgg aaagagtaaa
     9721 agaaaactgc ggcatgaaat ctgaaaagat ctgctgcgtt ccttttgacc tgtcagctac
     9781 tgaagatata cgggaaataa cagaaaaaca gatcaaccgg acaggaaaaa ttgactatct
     9841 cctcaacatc ggcggagtga gccagagatg ccggattgag gaaacaccat tgtggctcga
     9901 caggaaaata tttgaaatca actatttcgg aactatagca cttaccaagg ctgtccttcc
     9961 tttcatgatc agacaaaaaa gcggtcacat catcgctacc agcagcatat caggaagatt
    10021 cgggttccct ttgagatcag cctattcagc atcaaaacag gcaattcatg gtttcattga
    10081 aaccctttac cttgaaaaca aagccaataa catacgtgca tctgtaataa tacctggaag
    10141 ggtcagaaca aacatctcat tgcatgcact aaacagtgat ggcagggaac atggtgttat
    10201 ggatccggga caggaaggcg gaataccggc cgaaaaggct gcagcgataa tcatcaaggg
    10261 cattatcaga aacaagaggg agatcctggt tggagggaaa gagcttcttc tcttgcatgt
    10321 aagaaggacg gcaccatggc tctttttcag aattgccgat aaaatcgata caatgtaata
    10381 ctaactaaaa cctgaagaat aatggaatat gtcattggcg gaatacagca gatcggaatt
    10441 ggtgtcatga atattgagga atcatggaaa tggtatattg acatgtttgg aatggattgc
    10501 aagatttttg acgatgactc ggaagcaaat cttatgtcgc gctacaccgg aggaactccg
    10561 gtaaaacgac gtgcggtgct tgcctacaat cttcagagcg gcagtggatt tgaggtatgg
    10621 cagtacaagg gacgaaaacc tgcggaaaaa tcaaccatga tcaaactggg cgacctgggt
    10681 atccttatct gcaaaattaa aaccaggaat ctgaaaaaaa cctttgaata tttcggtagg
    10741 aaaaatgtca atatcctggg aaaacctgtc cgtgatccgg caggctcgga atcctttttc
    10801 atgaaggatc cctacaataa ccttttccag gttatggaat tcaaccactg gttcattgat
    10861 gagaaaaaac ctacgggggg ctcgtgcgga gcgattatcg gggtatccga catagataaa
    10921 gccagagtgg tttactctgg aatccttgga tacgatgaag tggtttacga ctcaacggga
    10981 atttttcccg atcttgcatg cctgcccggg ggtgataaaa aaatccgcag ggttctcctg
    11041 aaatgctcga aacctttcaa tggtcctttc atcaaggtat tcgggcaaag tgtcattgaa
    11101 ctcgtatgcg accctgaaga atccggtgaa aaaaccttga gcggccggct gtggggcgat
    11161 ccgggattca tacacctttg ttacgatata tccggaattg attccctcat ggaaacctgc
    11221 aacaaagctg gtcatccgtt cacggttgac agcaggagca accaaaatgg cgacagcttt
    11281 ggcatgggag ctgcggcagg ccactttgcc tatatcgagg accccgatgg tacgcttatt
    11341 gagtttgtgg aaactcacaa actccctgta ctgaaaaaat tcggatggta tattgatctg
    11401 aaaaaaagaa ggacatacaa accattgcct gactggatga tcaaaatgct cagattttca
    11461 aaggtctaga tcttcctccc caggcttgct gccctaagta ccctcatgat attgcctccc
    11521 catatcttgg aaatatcagc tttagaatat cccctgcgca gcaactcaat tgtaatattc
    11581 cgcatttctg cagctgattt gcagccttca acacttcctc ctccgtcgaa atcggttcca
    11641 atacccacaa aatctatacc tgccacctga accacatgat caatatgatc aacgacatcc
    11701 ctgacggtgg caagcttctc atacttctcc tttatcttta cgtattcatt gaacataagc
    11761 tccttcttct catcagaaag cttttcgtat tctccgaagg tatttctaag ttctttcagc
    11821 ttttcttcaa attcaggatt cggcgcagga gtcttcacat atcctgaaag caggcatatc
    11881 tggaccacgc caccgttagc cttgagggcc agaagcatgt catcggtcag gttcctcggg
    11941 ctctcgcaaa gtgccctgca gcacgagtgt gaagctatca ccggagccgt ggatatctga
    12001 agaacgtcgt aaaatgcttt gtcagagata tgggaaacat caaccatcat tccctggcgg
    12061 ttcatttccc ttaccacctg ctttcccaaa tcagaaaggc cgttgtgctc gggtccggcc
    12121 ggatcagtcg atgaatcaca aatatcattg ttcctggtgt gacaaagggt tatatacctg
    12181 gctccaagat cataata
//
LOCUS       DAOOYG010000077        12114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_5491_length_12114_cov_5.454681, whole genome shotgun sequence.
ACCESSION   DAOOYG010000077 DAOOYG010000000
VERSION     DAOOYG010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5491_length_12114_cov_5.454681"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PK288_07660"
     CDS             complement(<1..453)
                     /locus_tag="PK288_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18839.1"
                     /translation="MKILAGAVALLTVCYLFGCSGEGITNSSGKTDVKAVSLEPGNRP
                     LLSTGTGNTVARICIVSAKPGKAAGIRKMILGFSKESRLEDLAFVRVICEADPGEGIA
                     RDSLYKFIIRGNKVIIKGRFALQEGENRLSIVAGIRENANLDNVLELEK"
     gene            complement(471..1811)
                     /locus_tag="PK288_07665"
     CDS             complement(471..1811)
                     /locus_tag="PK288_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18840.1"
                     /translation="MRDTRRSFLKKAAAGSALLALEGGFSGLTAAGYRRTSGANSLIR
                     VAVFGCNSRGASLAGTFARQSGTEVKYICDVEENALKKGVDAVIGAAGKEPRTEKDFR
                     RILNDKDLDALVIAMPDHWHSPATIMGCAAGKHVYVEKPMSHNPYEGELVIRAVEKHK
                     TIVQVGTQRRSWSVLAQGLNELKNGIIGRVYMAKAWYANTRGPIGVGRTVPVPAGLDY
                     ELWQGPAPRKPFRDNLIHYNWHWFWNWGTGEALNNGTHHLDIIRWGLDLDYPSKVSSA
                     GGRYHYHDDWEVPDTQIITWEVPGITITWEGRSCNGGLVDNRSHGVIFYGEQGTLHTG
                     DNSYTVFDNTNKLVKEVKSDVIISESLNTTSPGEALDAVHVKDFLDNIRNGRTPSADA
                     QNGHKSALWLHLGNISQRVGRSLALDPSNGHITGDSEAEALWRREYENGWQPLV"
     gene            complement(1855..2694)
                     /locus_tag="PK288_07670"
     CDS             complement(1855..2694)
                     /locus_tag="PK288_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPM18841.1"
                     /translation="MKSRRNFLKLTTAGLIAAGASPVRVSAAALPANYSFAGPLTLGM
                     AGYTFREFTVEQTIEIMKKTGVTFLSLKDFHMPMNSTKQQIDSVIGKFRSAGITVYTV
                     GVIYMKTPEEVDRAFDYAKMAGVSMIVGAPDYDLLPYVEKKVREYDFRLAIHNHGPDN
                     PLFPNATDIWDHVKNLDPRMGICIDIGHTMRDGADPAADILKYASRIYDVHIKDVDKA
                     TKEGTTVEIGRGIIDIPGVISALRKIGYTGKCSLEYEKDMHDPLAGIAESLGFFRGVM
                     ACR"
     gene            complement(2958..4883)
                     /locus_tag="PK288_07675"
     CDS             complement(2958..4883)
                     /locus_tag="PK288_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HPM18842.1"
                     /translation="MKSRIRPVGIYAALLYRMALMMFIFTLCRTGFYLFNHKMFPGLT
                     GAEFLSILRGGLMFDISALVYINVLFILAQIIPFEVRYNRIYQDISRYLFFIINGLAI
                     AINCADFVYYRFVLKRATAEVFRTFQNESGLLRLLFRFQIDYWPVVLFGLALIFLMIF
                     LYNRIKTRSPLPHTRIRAFVLNLFAVPVIIIFFIVAARGGYRHSTRPVSISNAAKYVK
                     NPRDAAIVLNTPFSLIRTFDKKSLIRYSFFDDKKLNELYNPHYKPGKKGEFNPCNVVV
                     IILESFSREYIGALNRDLDGGSYRGYSPFLDSLIGQSLTFTVSLANGKKSIDAMPSIF
                     ASIPSLETPYVISHYANNTINGLPRLLKDKGYYSAFFHGAPNGSMGFDSFARMAGFDD
                     YFGLDQYKDKNDFDGMWGVWDEPFLDFFCKTIDAFTQPFFASVFTVSSHHPFRVPEKY
                     KNKFPKGPAPILEVIGYTDYALQKFFAASQERPWFSNTLFVITADHTNETVRKEFQND
                     FGSYCIPIIFYKPGSCLNGIKNKIAQQIDIMPTVLAYLNYDGEYIAFGNNLFDDSAES
                     FAFNTNGSTYHLYMKDHMIEMIDNKVVALYNFINDRMLQNNLKNSNDSLRTMMENKLR
                     AVIQSYNNRLIDNNMIP"
     gene            complement(5191..5841)
                     /locus_tag="PK288_07680"
     CDS             complement(5191..5841)
                     /locus_tag="PK288_07680"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HPM18843.1"
                     /translation="MSETSTFDFNAFIKESKETLLNPKAYFSTMKTSGGWAEPLIKAV
                     IYGAVAGIIAFIWSIVGLSGLKAGMFGGAIGAMALVWSIVGAVIGLFIGAVILLIISS
                     ICKGSTDFEANGRVVAASMVIMPINALLAFTAAISSWLGLIISLAVSLYALYIMYHGL
                     VESLKAKPETARIVMYILAALLVLFMLLGMSTRNKMNKYLDNPELKEMLQDLEKQG"
     gene            complement(5956..7848)
                     /gene="speA"
                     /locus_tag="PK288_07685"
     CDS             complement(5956..7848)
                     /gene="speA"
                     /locus_tag="PK288_07685"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HPM18844.1"
                     /translation="MRKWRIEDSAELYNINGWGVNYFSINSRGNVEVRPQKEGAAVDL
                     RDLLDELILNDVSTPVLVRFPDILDDRIITISRCFKTASEEYDYKAQNFIIYPIKVNQ
                     MRPVVEEIVSHGKKFNIGLEAGSKPELHAVIAINTDPESLIICNGYKDEDYIELALLA
                     QKMGKRIFLVIEKLNELRLITTIAKRLKVKPNLGIRIKLASVGSGKWEESGGDISKFG
                     LTSSELLEAIDFLERNKMRDCLRLIHFHIGSQVTKIRRIKVALREASQFYVQLRKMGF
                     NVEFVDIGGGLGVDYDGTGSSNSESSVNYTIQEYVNDSVSTFVDASNKNNIPHPNVIT
                     ESGRSLTAHHSVLIFEVLEATTLPSWDVEDTISDSDHELVKELYQLWENVNQPRMLET
                     WHDAQQIREEALDRFSLGLIDLKTRAQIESLFWSVARKVYQLANMIRHVPEEIRQISR
                     MLSDKYFSNFSLFQSLPDAWAIDQIFPIMPIHRLNEEPVRTATIQDITCDSDGKIDNF
                     ISTRNSSYHLPVHPLKGKEPYYMGVFLVGAYQEILGDLHNLFGDTNAVHVSVGEDGYK
                     IDQVIDGETIAEVLDYVQYNPKKMVRTVETWVTSSVKTGIITLEEGKEFLSNYRSGLY
                     GYTYLE"
     gene            complement(7978..9606)
                     /locus_tag="PK288_07690"
     CDS             complement(7978..9606)
                     /locus_tag="PK288_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HPM18845.1"
                     /translation="MNKLLRFVFFLIIILVTAEGSSGQDLLTVAERTGYKSTSGYSDV
                     VTFIDGLRALSPFIRVESMGRSVNGHDIPLLVIGDPFLQASGADRKNKLVVYIQAGIH
                     ADEVEGKEASLMFARDILRQGDPVLFRNLLILICPIFNIDGNEKISPLHRTYQNGPVN
                     GVGVRYNGQSLDLNRDAMKAESPEIRGLYSNVFNKWDPEIFMDCHTTNGSYHVEPVTF
                     TWMVNPNGDTALIGYMRRKMMPGLSAALVNKYGIENCFYGEFVNMLNPGLGWIYEISQ
                     PRYLTNYYGLRNRLAILNENYVYADFRSRVNGCYSLMRTLIEYAVANKPEIKDLLNSA
                     DRKTVMRGLDPGPEDSLGIVFDVRSAGDVTIRTYEAELVQGENGWNSYRRTDRQIDVT
                     VPYLIDYYATAKARFPFAYVLAVNDPEIIELLRIHGIRMEKLEKPVSVWVEKFEISRL
                     TGAARLNQGHYTNTLEGSFRRENTTFPEGTIIIRTSQPLAGVAACLLEPQSDDGLVVW
                     NFLDRYLVPQWGPGYNDLPVYKITGRIDLPTVDF"
     gene            9787..9978
                     /locus_tag="PK288_07695"
     CDS             9787..9978
                     /locus_tag="PK288_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPM18846.1"
                     /translation="MNKGTVKFFNVTKGFGFIKDADSDNEYFVHATGLIDKIKENDEV
                     TFELAEGKKGINAVNVKLS"
     gene            complement(10371..11129)
                     /gene="pgl"
                     /locus_tag="PK288_07700"
     CDS             complement(10371..11129)
                     /gene="pgl"
                     /locus_tag="PK288_07700"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPM18847.1"
                     /translation="MKASVRIFNSRFELAESMALDIVSLARNREAEGRPFAMAIPGGS
                     TPRILFTVLGEHFGGSVNWNNVHIFWVDERCVPPSDAASNYGMAKESFLDRIEIPGQN
                     VHRIKGEADPEKEAIRYSAALRENTEIRNGIPGLDFMLLGMGEDGHVASIFPGNEAFF
                     HSDKPCHVARHPQTGQKRITLSGKIINNSGKIYFMVTGKDKAEIVRRVLCTDSKQQPV
                     PASFVCPAGGAAVWYLDREAASLIPSGTADGLIL"
     gene            complement(11293..>12114)
                     /locus_tag="PK288_07705"
     CDS             complement(11293..>12114)
                     /locus_tag="PK288_07705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HPM18848.1"
                     /translation="SGALRDMVQNHLLQMVGLTAMEPPSSLDADAIRNEVLKVFQSFQ
                     PIKESEVPGQVIRGQYTGSRIRGEAVPGYRHEKNVNIHSRTETYVAMKFFINNWRWGG
                     VPFYIRTGKRLPTRVTEIVIHFKPTPHHLFQRVVGNRSGNQLIIRIQPDEGILLKFDM
                     KEPGAGFNVKNVNMDFHYKDLADVRVPSAYERLLFDVMEGDSTLFSRDDEVEMAWKFV
                     EPVQRAWKNNPEIKIYGYPAGTWGPEISNSLIEGDGLSWRYPCKNLADEELYCEL"
BASE COUNT         3197 a         2916 c         2551 g         3450 t
ORIGIN      
        1 cttttcaagc tccaaaacat tatcaaggtt ggcattctcc ctgatgcctg ccacgatgct
       61 cagcctgttc tcaccctcct gcagggcaaa cctgcctttt attattacct tgttgcccct
      121 tataatgaat ttataaagag aatccctggc aatcccctcc ccgggatctg cctcgcagat
      181 gacccggaca aaagccaggt cttccaatct gctctctttc gagaatccaa ggatcatttt
      241 tctgattcct gctgcctttc ccggtttagc tgatacaatg catatcctgg ccacagtgtt
      301 tcctgtaccg gtacttaaaa gtggtctgtt tcccggttca agggaaaccg ctttcacatc
      361 cgtttttccc gaactgttag taataccttc acctgaacat ccgaaaagat aacagactgt
      421 caggagtgca actgcaccgg ctaatatttt catacgcggc agattatatc tcagacaagc
      481 ggctgccatc cattctcata ctctctgcgc cacaatgctt cagcttcgct gtccccggtt
      541 atatgtccgt tggatggatc aagtgcaagg gagcgcccca ccctctgcga aatatttcca
      601 agatgaagcc acagagcgct tttatggccg ttttgtgcat ctgccgaagg agtccttcca
      661 tttcttatat tatcgaggaa atccttgaca tggacggcat cgagtgcttc gcccgggctt
      721 gtagtattga ggctttcgga gataatgaca tctgacttca cttccttcac cagcttgttc
      781 gtattgtcga aaacagtata actgttatcg cctgtgtgaa gggtgccctg ttcaccataa
      841 aaaataaccc catgcgaacg gttgtcgacc aatcctccgt tacagcttct tccctcccag
      901 gtaattgtta ttccaggcac ttcccaggta atgatctggg tatcgggaac ctcccagtcg
      961 tcatgataat gatagcgacc ccccgccgaa cttactttgg aaggataatc aaggtccagc
     1021 ccccacctga taatatcaag atggtgtgtt ccgttattca gcgcttctcc cgtaccccag
     1081 ttccagaacc agtgccagtt ataatgaata agattatccc tgaatggttt tcttggagca
     1141 ggtccctgcc acaattcata atcgagtcct gccggaacgg ggacagttct gccaacacca
     1201 atcggacccc gcgtgtttgc ataccaggct tttgccatgt ataccctgcc aatgataccg
     1261 tttttaagct cgttcagccc ctgggccagt acggaccacg atctccgttg ggttccgacc
     1321 tgaactatgg tcttgtgttt ttcaactgcc ctgataacca gttccccctc atacggatta
     1381 tgactcattg gcttttctac ataaacatgc tttcctgcag cgcatcccat gatagtggcc
     1441 ggtgagtgcc agtgatcggg catggctatg accaacgcat caagatcctt gtcattaagg
     1501 atacgtctga aatccttttc agtccggggc tcctttccgg ctgcgccaat gactgcgtca
     1561 acacccttct tcagggcatt ctcttccaca tcgcagatat acttcacctc tgtaccggat
     1621 tgcctggcaa atgttcccgc cagcgaagca ccacggctgt tgcatccgaa aactgcaacc
     1681 cgtataagac tgtttgcgcc tgatgttctt ctgtaaccag ctgccgtcag gcctgaaaaa
     1741 ccgccctcca aggcaagaag agcacttccg gctgctgcct tttttaaaaa cgatcgtctg
     1801 gtatctctca tgaattcatc gaatgaataa ttgattctgc ttttatgccg caggtcagcg
     1861 gcatgccatt actcccctga aaaagcccag agattctgca atacctgcga gcggatcgtg
     1921 catatccttt tcatattcaa ggctgcactt gcccgtataa ccgatcttcc tcagagctga
     1981 tatcaccccc ggaatatcga ttatccctct tccgatttca actgttgtgc cttcctttgt
     2041 cgctttgtca acatccttaa tatgtacatc atatatgcgg ctagcgtatt ttaatatgtc
     2101 agccgcaggg tccgcaccat ccctcatggt atggcctata tcgatacaga tccccatccg
     2161 cggatcgaga ttctttacat ggtcccatat gtcagttgca ttggggaaca acgggttgtc
     2221 cggaccatga ttatgaattg cgagcctgaa atcatattcc cttacctttt tctcgacata
     2281 gggaagtaag tcataatcag gggctccgac gatcatactg acccctgcca tttttgcata
     2341 gtcaaatgca cggtcaactt cctcaggagt tttcatatat atgactccaa cagtataaac
     2401 tgttattccg gcacttctga acttgcctat aaccgaatcg atctgttgtt ttgtactgtt
     2461 catcggcata tgaaaatcct tgagggagag gaatgtgact ccggtctttt tcataatctc
     2521 aatcgtctgt tcaacggtga attccctgaa tgtataacca gccataccaa gggtcagcgg
     2581 accggcaaat gaataattag ccggtaatgc agcagcagaa acgcggactg gagaagctcc
     2641 ggcggcaatc aaacccgctg ttgtcagctt caaaaaatta cggcgtgatt tcataatata
     2701 tctattaaat gatttaaata cctgaaaatc agatttgtca ctgatccgga aagttcatgg
     2761 atatcatcaa ggatgttttc attgaagcct tgtaataatt ggtttccgtt taataaagga
     2821 tgtcagctga atttgtgatt tcattatata caaatataca gataccgtta aaaaataagt
     2881 aaaaatcctg aaatttatga atatgcaagg gcattggccc ggataaaagc gttcagttgc
     2941 tggttccccc ttcctgatca ggggatcata ttattatcta tcaggcggtt attatagctc
     3001 tggataacgg ccctgagctt gttttccatc atggtacgca ggctgtcgtt gctgtttttg
     3061 agattattct gaagcatcct gtcattaatg aagttataaa gagccaccac cttattatca
     3121 atcatttcaa tcatatgatc cttcatatac aggtgatatg tgctgccatt tgtattgaat
     3181 gcgaatgatt cggctgaatc gtcaaataaa ttatttccga acgcaatata ttccccgtca
     3241 taattgagat atgcaagcac tgtgggcatt atgtcaatct gctgggcaat tttgttcttt
     3301 atcccattca ggcagcttcc cggtttgtag aaaataatgg gaatgcaata tgatccgaag
     3361 tcattctgaa actctttacg gaccgtttca tttgtatgat cggctgttat cacgaaaaga
     3421 gtattactga accaggggcg ttcctgactg gctgcgaaga atttctgaag ggcataatcg
     3481 gtgtaaccta tcacttcgag gataggagcc ggacctttgg gaaatttgtt tttatatttt
     3541 tccggaaccc tgaacggatg gtgggaagaa accgtaaaga cggaggcaaa aaatggctgg
     3601 gtaaaggcat ctatcgtctt acagaagaag tcgagaaaag gttcgtccca tactccccac
     3661 attccgtcaa aatcattctt gtctttatac tggtcaaggc cgaaataatc atcaaacccc
     3721 gccattctgg caaaggaatc aaatcccatg gaaccattag gtgcgccatg gaaaaacgcc
     3781 gaataatatc ccttatcttt cagcagccgg ggcagtccgt taatggtgtt attggcataa
     3841 tgcgaaatca catagggggt ttcaagcgaa gggatcgatg caaaaataga aggcatggca
     3901 tcaatcgatt tctttccgtt tgccagggat accgtaaatg taaggctttg tccgatcaat
     3961 gagtcgagaa agggtgaata acccctgtaa ctgcctccgt caaggtccct gttcagggct
     4021 ccgatatact cccttgaaaa actttcaaga ataattacaa ctacattgca gggattgaat
     4081 tcgccttttt ttccgggttt gtaatgagga ttgtaaagtt cattgagttt tttgtcatcg
     4141 aagaagctgt agcgtatgag ggatttcttg tcgaaggtcc ggataagact gaatggtgta
     4201 ttcaggacga tcgccgcatc gcggggattt ttcacatatt ttgcggcatt gcttatgctt
     4261 acgggcctgg ttgaatgcct gtaacctccc cgggctgcaa caataaagaa aataattatc
     4321 acggggaccg caaataaatt caaaacgaat gcccggatcc gggtatgcgg caaaggactc
     4381 cgggtcttaa tccggttgta cagaaatatc attaaaaaga tcagggctaa accaaacagg
     4441 accaccggcc agtagtcaat ctggaacctg aataacagtc tgagcaggcc cgattcattc
     4501 tgaaaagttc tgaaaacctc agcagtggcc cttttaagaa caaaccggta atatacaaaa
     4561 tctgcgcaat ttatggcaat tgccagtccg tttatgatga agaacaaata acgtgatata
     4621 tcctggtaaa ttcggttgta tcttacttca aaaggtatta tctgggctaa aatgaacaac
     4681 acattgatat ataccagggc cgagatatcg aacatcagcc cccccctgag aatgctaagg
     4741 aattctgccc cggtcaggcc ggggaacatt ttatggttga ataaataaaa tcctgtcctg
     4801 catagtgtaa aaatgaacat catcagtgcc atcctgtata gcagagctgc atatatgcca
     4861 acgggtctga tccggctttt catccctgac tcatataatc cctttcatta tccggaaagg
     4921 gcggattaat agaactgtca aagataaaaa taactgatct taaaagtcat gctgtcgtga
     4981 aaatagggaa atgccccgtg atgtccggtc agtctgtggc agcatgcgtg aagtaatctt
     5041 caccggtggc ttaagggatg acaaaaaaca gttattccgg ctgcagtcac ggtgaagacc
     5101 gtaagttaca gcacaggtaa ggcgataacg gtaatcacaa caaaagtgaa cctgttacgc
     5161 tgccccggaa tctattataa atttctgact ctaaccctgc ttttcaaggt cctggagcat
     5221 ttccttaagc tcaggattgt caaggtattt attcattttg tttctggtgc tcatcccgag
     5281 aagcatgaaa aggacgagca gggctgcaag gatatacatg acgatcctgg cagtttcagg
     5341 tttggccttc agcgattcaa caaggccgtg atacataatg taaagggcat agaggctgac
     5401 ggcaagactt attatcaatc caagccatga acttatcgct gcggtaaagg caagcagtgc
     5461 atttatcggc atgataacca tcgaagcggc aacaaccctg ccattagctt caaaatcagt
     5521 gctccccttg cagatcgatg aaattatgag cagaatcacc gcaccgatga acaatccgat
     5581 tacagcgcca acaatggacc agacaagtgc cattgcccca atggctccgc cgaacattcc
     5641 ggctttcagt ccgcttaatc ccacaatact ccagataaac gcaataattc cggccacagc
     5701 tccgtaaata accgctttga tcaatggttc cgcccatcct ccggaagttt tcattgtgga
     5761 gaaataagcc ttgggattca gcagtgtttc ctttgattcc tttataaagg cattgaaatc
     5821 gaaagtagaa gtttcgctca tagcaagtaa attaaaaggt taattgatat gatcagttaa
     5881 gattgaaata taaaacgtca agttagttat tttttttaac ctgacctata ctttgagtga
     5941 aatattagaa ttcttctatt ccaggtatgt gtatccgtac agtcccgaac ggtaatttga
     6001 aaggaattcc tttccttcct ccagcgttat tattcccgtt ttaactgatg atgtaaccca
     6061 tgtttccacg gtcctgacca ttttcttggg attatactgc acataatcaa gaacctcagc
     6121 gatggtctca ccatcaataa cctgatcgat cttataacca tcctcgccaa cgctgacatg
     6181 aactgcgttg gtatcaccga atagattatg cagatctccg agtatttcct ggtatgcccc
     6241 cacaagaaaa acacccatgt aatatggttc ctttcccttc aaagggtgta ccggaaggtg
     6301 gtaagatgaa ttcctggttg aaatgaaatt atcaattttg ccgtcggaat cacaggttat
     6361 atcctgaatg gtagctgttc tgaccggttc ctcattgagc ctgtgaatag gcattattgg
     6421 aaatatctga tcaattgccc atgcatcggg caatgactgg aacaacgaaa aattgctgaa
     6481 gtatttgtct gaaagcattc gtgagatctg cctgatttct tccgggacat gcctgatcat
     6541 attggccagc tggtaaacct tgcgggcaac tgaccagaaa aggctttcaa tctgtgccct
     6601 ggttttgagg tcaataagtc caaggctgaa cctgtcgagt gcttcttccc tgatctgctg
     6661 ggcatcatgc catgtttcca gcatccgcgg ctgatttaca ttttcccata gctgatacag
     6721 ctccttcacc agttcatgat cgctgtcgct tattgtatct tccacatccc atgaaggcag
     6781 cgttgttgcc tcaagcactt cgaatatcag gaccgaatga tgcgccgtaa gtgaacgtcc
     6841 cgactccgtt atgacgttgg gatggggtat gttgttcttg ttcgatgcat caacgaaagt
     6901 gctgactgaa tcattcacat attcctgaat ggtgtagttg acactactct cgctgtttga
     6961 cgatcctgtt ccgtcatagt caactccgag cccgccgccg atatcgacaa attccacgtt
     7021 gaatcccatt ttccggagct gtacataaaa ctgagaggct tccctcaggg caacttttat
     7081 tcttcttatc ttggttacct ggcttccgat atgaaaatgg atcagcctga ggcagtcgcg
     7141 cattttgttc cgttcgagga aatcaatagc ttcaagcagc tcgctggaag taagaccgaa
     7201 tttgcttata tccccgcctg attcttccca tttaccgctg ccaacgctgg ccagttttat
     7261 cctaataccc aggttgggtt ttactttcag tctttttgcg atagttgtta tcagcctgag
     7321 ttcattaagc ttttcaatga ccaggaatat ccttttaccc attttctgcg ccaggagtgc
     7381 aagctcaata tagtcttcat ccttataacc gttgcatatg ataagcgact ccgggtcggt
     7441 attgatggca atgacagcat gaagttcagg ttttgaacct gcctcaaggc ctatattgaa
     7501 ttttttccca tggctaacga tctcttcaac aacgggcctc atctggttaa ccttgatcgg
     7561 gtagattata aaattctggg ccttgtaatc atattcttcc gatgctgtct taaagcacct
     7621 tgagattgtg attatcctgt catcaagaat gtctggaaac cttacgagga ccggagtgga
     7681 aacatcattg agtatcagct cgtccagaag atcccttaaa tccactgctg cgccctcttt
     7741 ctggggtctg acttcaacgt ttcccctgct gtttattgaa aaatagttga caccccagcc
     7801 atttatgttg taaagctcag ccgagtcctc gatcctccat tttctcattt caagtcactt
     7861 ttcttattat tgatctggca ttctgaaatt tcccgcaatt ttatgaaata aacattaaag
     7921 agtgccaata ataatcaatt atttatcagc gcaggcagtt ttcaggtcat tttggtttca
     7981 gaagtcaact gtcggcaggt caatcctgcc ggttattttg tacacgggca ggtcgttata
     8041 acccgggccc cattgcggaa cgagataccg gtcgaggaaa ttccacacaa ccagaccgtc
     8101 gtcggactga ggttcaagaa gacaggcagc cacccctgca agcggctggg atgtccggat
     8161 tataattgta ccttcaggaa aggttgtgtt ttcccttctg aaggaacctt ccagggtatt
     8221 tgtataatgt ccctgattca acctcgctgc tcccgtaagc ctgcttattt caaacttctc
     8281 tacccagact gataccggct tttccagctt ctccatcctg ataccgtgta ttctcagaag
     8341 ctcaataatt tcaggatcat tcacagcaag cacataggca aaagggaacc tggccttcgc
     8401 cgtggcataa taatcaatca gataaggtac agtaacatca atctgcctgt cggtcctacg
     8461 gtagctgttc catccgtttt cgccctgaac cagttcagcc tcgtaggttc tgattgtgac
     8521 atccccggcc gaacggacat caaatactat tcccagtgaa tcttcaggtc ccggatcaag
     8581 tcccctcatc acggtcttcc tgtcggctga attcaacaaa tcctttattt ccggtttatt
     8641 ggcaaccgcg tactcaataa gcgtccgcat cagactgtag cagccattaa ccctcgatct
     8701 gaaatcggca taaacatagt tttcattcag aattgccagc cggtttctca ggccataata
     8761 atttgtcaga taccttggct gtgaaatctc ataaatccac ccgaggcccg ggttaagcat
     8821 attcacaaat tcaccataga aacagttttc tattccgtat ttattcacca atgcagcaga
     8881 caatccgggc atcattttcc ttcgcatgta accgataagg gcggtatcgc cgtttggatt
     8941 caccatccat gtgaaggtaa cgggttcaac atgatacgat ccgtttgttg tatggcaatc
     9001 catgaatatt tcaggatccc atttattgaa tacgtttgaa taaagtcccc ttatctcggg
     9061 agattcagct ttcattgcat ccctgttaag atcaagcgac tggccgttat acctcactcc
     9121 caccccgttg acggggccgt tctggtatgt ccggtgcagc ggggatatct tctcatttcc
     9181 gtcaatattg aatatgggac atatgagtat caacaggttc ctgaaaagaa cggggtcgcc
     9241 ctgccttaga atgtcccttg caaacatgag agacgcttcc ttgccttcaa cctcgtcggc
     9301 gtgtatgcct gcctggatat acacaaccag cttatttttc cggtcagctc cggaagcctg
     9361 aaggaacgga tctccaatta caagtagtgg tatatcatgg ccgttcactg acctgcccat
     9421 tgactcaacc ctgataaaag gcgacaaggc tctgagtcca tcaatgaatg ttacgacatc
     9481 actgtatccg gatgtggatt tgtaaccggt gcgttctgcc actgtcagca ggtcctgacc
     9541 ggatgatcct tcagcggtaa ccagtattat aatcagaaag aaaacaaatc tcagcagttt
     9601 attcatcccg gaaaacaatt gatttaaagc attaaaatta gaccttttcc aacaaataca
     9661 gtgtcgctga aaaagacaaa atgagtatca atccgggaca actgtcatat gcctttctta
     9721 aaagaattgg taaaattgaa attatatgcg tacattagcg gccttaattt tttaaaacat
     9781 attaaaatga acaaaggaac agttaagttt tttaatgtaa caaagggatt cggattcata
     9841 aaagatgccg attcggataa tgagtatttc gttcatgcaa caggtctgat tgacaagatc
     9901 aaggagaatg acgaagttac ttttgagctt gcagaaggca aaaaaggaat aaatgcagtg
     9961 aatgtaaaac tatcttagac ccgaaacatc acagtttatt ataccggagg ctgaagtatc
    10021 agcctccttt ttttgcgatt tatcgaaggg aaccggttgg aaaggaagtc aatcgggcat
    10081 tgtttccgag tgcctgccgg cggattgctt ctctaaccgg cagatcggca aagatcattc
    10141 ctcattatca tttacaatcc tccggcaata tgcagcctgc agttaaatcc cgggaaggat
    10201 cactgcttgc tctggtttgc cttttttgct tttttagctg cccttttttc ctttaatgat
    10261 ttggtggcct gtttcttcac ttttgtctgt gccttggctt tttcgtgtga catgacaata
    10321 ggtttttata tttaaacaag atagccaaat atataaaaac ctgtcatatc ttacaatatt
    10381 aaaccgtctg cggttccgga aggaatcagc gaagccgctt ccctgtcgag ataccatacg
    10441 gcagctccgc cggccggaca aacaaacgat gccggaacag gttgttgctt actatcggtg
    10501 cacaaaactc ttctgacaat ttctgcctta tctttcccgg taaccatgaa gtatattttc
    10561 ccggagttat tgataatctt accggaaagg gtaatcctct tctggcctgt ctgcggatgc
    10621 ctggcaacat gacacggttt atcggaatgg aaaaaagcct catttccggg gaaaattgaa
    10681 gccacatgac catcctcgcc cattcccagc agcataaagt ccagtccggg gatcccattc
    10741 ctgatttcgg tattttccct tagtgccgcc gagtaccgta tcgcttcctt ttccgggtca
    10801 gcctcaccct ttatccggtg tacattttgc cctgggattt caatcctgtc gaggaaggat
    10861 tcctttgcca ttccgtaatt gctggcggca tcggatggag gtacgcatct ttcatcaacc
    10921 cagaatatat gaacattatt ccagtttaca gatccgccaa agtgctcacc caataccgta
    10981 aacagaatcc gtggagtgct accacccgga atggccattg caaagggccg gccttcagcc
    11041 tcccggttcc gtgcaaggct tacaatatcc aacgccatgc tctcggccag ctcaaacctg
    11101 gaattgaaaa ttcgtacaga agctttcatt ggtatctgtt tatggtttca gttaaaaaat
    11161 tcagacaaaa ggaggcatgg attaagtaat aacatctccg gtaatgctgg tctggttgta
    11221 aaacatctgc tacatttgaa ataatgcatc cgagtgagtt caattaaacg ccggtatcag
    11281 ctgtgaaata attcacagct cacaatacag ttcctcgtcg gccaaattct tgcatggata
    11341 tctccacgat aggccgtcgc cttctatcag ggaatttgat atttcagggc cccatgttcc
    11401 tgcaggatac ccatagatct ttatttccgg attatttttc catgcgcgct gaacgggttc
    11461 gacgaatttc caggccattt caacttcgtc atccctggaa aagagggttg agtcgccttc
    11521 catcacgtcg aagagaagtc gttcgtaagc agaaggtact cttacgtcag caagatcctt
    11581 gtaatgaaaa tccatgttca cattcttcac attgaaaccc gctccgggct ccttcatgtc
    11641 aaatttgagg agtattccct catcgggctg gatcctgata ataagctgat ttcccgaccg
    11701 gttgccgaca actctctgaa aaagatggtg aggtgttggc ttgaaatgga tcactatctc
    11761 ggttactctt gtcggaaggc gctttccggt acgtatgtag aacgggaccc ctccccagcg
    11821 ccagttattg ataaaaaatt tcatggcaac ataggtttca gtcctggaat ggatattaac
    11881 atttttctca tgcctgtatc caggtacggc ctctcctctt atccttgaac cggtgtattg
    11941 ccctctgata acctggccgg gcacctctga ttccttaatg ggctgaaacg actggaatac
    12001 cttaaggacc tcattcctga tggcatcggc atcgagcgac gatggtggtt ccattgcggt
    12061 aaggcccacc atctgaagga ggtgattctg caccatatcc ctcagtgcac ctga
//
LOCUS       DAOOYG010000078        12066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_5527_length_12066_cov_6.827242, whole genome shotgun sequence.
ACCESSION   DAOOYG010000078 DAOOYG010000000
VERSION     DAOOYG010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5527_length_12066_cov_6.827242"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..327)
                     /locus_tag="PK288_07710"
     CDS             complement(88..327)
                     /locus_tag="PK288_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18849.1"
                     /translation="MKTISLIIDDSIYGETENILSKIKKPRNRYINEALDYYNKIQRR
                     KILESKLRKESALVKDESLSVLNEFEETDYASSQI"
     gene            complement(851..923)
                     /locus_tag="PK288_07715"
     tRNA            complement(851..923)
                     /locus_tag="PK288_07715"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(888..890),aa:Lys,seq:ctt)
     gene            complement(1165..2679)
                     /locus_tag="PK288_07720"
     CDS             complement(1165..2679)
                     /locus_tag="PK288_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM18850.1"
                     /translation="MSDLEKYFEPFRKNVIGIDACIETPYGTRKLVYADWIASGRLYG
                     PIEKRIINEIGPMVGNTHSESTATGKAMTDAYHMAQKIVKHHVNADENDVLIFTGTGM
                     TSAIAKLQRILGLKVPEQAINYCVFNHGEYNNCREVPNENRPVVFLTHMEHHSNHTSW
                     FETLADVVVLEPTADLTVNPDVLRREIARYRDRPLLIGSFTACSNVTGYIPPYYELAA
                     IMHQHNGYCFVDFAASAPYTEINMHPGNGLHQLDAIFFSPHKFIGGPGSAGVLVFNRQ
                     LYKNKTPDTPGGGTVKWTNRWGGYSYISDIEVKEDGGTPGFLQGIRAALAIRLKEEMT
                     TEKIQARERELRELAFKKLSAIKGLHILAENITDRLGVFSFYIDGVHHNLITKLLNDR
                     YGIQVRGGCSCAGTYGHFLLEVDIDLSREITDRIEAGDLSLKPGWVRLSLHPLMTDSE
                     LLYIAAAVEEIALNAAEWGKDYLYDRHTNEFNHRSYIEPVTAGKYGWFDNIGRD"
     gene            2789..3538
                     /locus_tag="PK288_07725"
     CDS             2789..3538
                     /locus_tag="PK288_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPM18851.1"
                     /translation="MTDLYHFLVPFVFIMSGVVLVILLFISAPYGKFLRRGWGPVIRS
                     KWAWMVMEFPSPALMILFFVTSADHGPVAGVFLSIWLLHYLHRTFVYPFRQSGRDKDF
                     PASLVVMALVFNIINGTINGYGIFHLRSYDVSYLAGGRFICGAVLFVTGFIINKWADE
                     KLRRLRSRNPDEYALPSGGLFDYISNPHYFGEILEWAGWATATWSLPGLAFFLFTFAN
                     LFPRAIASHKWYREHFSDFPGSRKALIPFIV"
     gene            3920..5194
                     /locus_tag="PK288_07730"
     CDS             3920..5194
                     /locus_tag="PK288_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPM18852.1"
                     /translation="MTSLTTFYLSGITGKEVFDADGEAIGRIRDLLISNAFSGTGDSN
                     QQRVIGIRLKVRKDIRYYSFDTFRVVKAREMISVTCSGLIELSEETVNNGLLLVENML
                     DRQIVDLNGRKLVRVNDVRFVTLPAGTFAVAVDIGIEGLLRRLGISIPIKRFLSLFNI
                     RIPAKFILWDDVQAIDFSNRNIKLSKSYAKLHMLHPSDLADILEDLGKKTSPHVFSAL
                     DEEKAADVLEELEPHAQIHIIESLPVPKVADVLEKMPADEVAYILDEIEDEKAELLLK
                     EMDSESSQEVRELLEYPDNSAGGLMTTDVLSFSPLLTVDEVIRELRIKKPEPSELYSL
                     FVTERNEELIGTFDLRDLVVADPETIIRNIMKTEPVSLFDYQRIDDIAELISKYNLLA
                     VPVVDKRNQLQGMVVVDDVIEDLISRRRTNKR"
     gene            5205..6461
                     /locus_tag="PK288_07735"
     CDS             5205..6461
                     /locus_tag="PK288_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HPM18853.1"
                     /translation="MFRTKFSKTRFYRNLTIFAALLGPGIITGSVDNDAGGITTYSVA
                     GAIYGYGMVWTLIPSLIVLLVIQEMNARMGIVTGKGLSDLIRENAGVKITFFIFIGLL
                     LSNIGNTTTEFAGVAGSMEIFGMSKYISVPLVAFMVWILVVKGTYKVAERIFLVFSAS
                     LLTYVVSALMGEPRWSEIGSAIIRPGIMLNTQSVTVIIALVGTTIAPWMQFYMQSSVI
                     EKGLKIKDYKYTVIDIVVGCIVTVVVAFFIMIACGSTLHPNGIRINEAKDAALALKPL
                     AGELASHVFAFGLFVASVFSATILPLATSFYICEAFGFETGLNKSWSEAREFYVLYTG
                     ILVVSAVIILIPNAPLIDISIWSQVLNGILLPVVLVCMILLVNDKKIMGEYTNNSLQN
                     IIGWGAVVIMTLLSLTLLVIPFFPGR"
     gene            complement(6464..7606)
                     /locus_tag="PK288_07740"
     CDS             complement(6464..7606)
                     /locus_tag="PK288_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPM18854.1"
                     /translation="MINSKGQYKKNNFAMVKERKKLTGLSIKFLTGILIFLSVNDFLC
                     AQDPFRGDQCIVRSEFIYDSSSVWFPGCHASTIAEMPGGFIAAWFGGTAEGNPDVGIW
                     ISRFTGGRWSIPEMVADGKTDSLRYPCWNPVLYNGDDEILLFYKVGPNPREWWGELKI
                     STDNGISWSAPRKLPDDILGPVKNKPVLLDNGKLLCPSSTEHDGWRVHMEFTPDFGIT
                     WERTGFLNNKDLYAIQPAILTHKTGRLQILCRTNRSRIFSSWSDDKGNSWSRLMPDSL
                     PNPNSGIDAVTLTDGRHFLVYNHLTKGRHQLNAALSDDGKTWKAALLLEDDSEGKEYS
                     YPAVIQSADGLIHITYTWRRRLIRHVVVDPARIEPRPMINGAWPRR"
     gene            complement(7638..9131)
                     /locus_tag="PK288_07745"
     CDS             complement(7638..9131)
                     /locus_tag="PK288_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPM18855.1"
                     /translation="MKLSPVDAVIFIILVLSNVIIGVSFYFRSRSSDQFVTAGGNIPA
                     WVAGMSIFATYLSSISFLALPGKAYMTDWNAFVFSLSIPVASFAAVRYFVPHYRKIGN
                     PSAYYFLEMRFGSWARIYASSCYVLTQLMRTGAILLLLALPLNALFGWNIKAIIILTG
                     LSVILYSMLGGIRAVVWTDTIQAFILITGALICAVYITLSMPGGAGQVFSIGASNGKF
                     SLGSFGTSLSESTFWVVLVYGLFINLQNYSIDQNYIQRYMTTGSDREAKKSALFGSLL
                     YLPVSMLFFYIGTALFAFYTAQPGRLAEELRPVSAGDRVFPYFIVHELPAGLTGLLIA
                     SIFAAGMSTISTSLNSTATIVLTDYFRKFAKRDPAEKASMRILYAATAATGFAGTAIA
                     LALTGVESVLDAWWALASIFSGGMLGLFLLGFLPVKAGKAEAVIGVTAGIIVIVWMSL
                     SPLLPDNGRLASFRNPVHSNLTIVFGTIVIFLTGFLISSYRARKKHS"
     gene            9242..10030
                     /locus_tag="PK288_07750"
     CDS             9242..10030
                     /locus_tag="PK288_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPM18856.1"
                     /translation="MKYGFFDSWSYDTPDKGKPGLCYRIIHHNRLYFTLKYATIVLRT
                     RKAAIKGVYDRKAWAESSYYIFRFIEKAGGKFHVSGMENIVACPSPVVFIGNHMSTLE
                     TMILPGLIAPHREVTFVVKEGLVRHPLFGHVMRSRDPVVVGRTDPRKDFEEVMTKGQE
                     LLSRGVSIVIFPQSTRSVEFRPEEFNSLGVKLAKKAGVNVVPVALKTDFWGQGKIIKE
                     LGPLDSSRPIFFRFGEPFAVTGSGREENQRVIDFVQSSLAEWEK"
     gene            10125..11777
                     /locus_tag="PK288_07755"
     CDS             10125..11777
                     /locus_tag="PK288_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPM18857.1"
                     /translation="METLDWIVLGLFCLGLIGIIIWVTRKKDETSTDYFLAGRDATWL
                     AIGSSIFASNIGSEHLVGLAGAGASSGMAMAHWEMHGWLILILGWFFVPFYARSGVFT
                     MPEFLEKRYTKGSRSVLSVISLVSYVFTKVAVTVYAGGIVFKQVFGIESMFGIDFFWI
                     SAVGLVVLTGIYTTLGGMKAVLWTSVLQTPILLIGSITVLIVGLVKLGGWSQMMEICR
                     AVPVNADGDTMTSLIRSANDPEYPWTGVILGSAIIGFWYWCTDQYIVQRVLSGRNQTE
                     SRRGAILGAAFKLSPVFIFLIPGMIAYALNYRGMISLPDSDSAFSVLVSELLPLGFKG
                     LVVGGILAALMSSLASLFNSSATLFTVDFYEKFRPQATEKELVRVGRMATVAVVILGI
                     LWIPVMKGLGKVLYAYLQDVQSLLAPGIAAVFLLGIASRKITPAAGFTGLLTGFVLGM
                     TRLGFKVFGTGLGTDSILYTVFLKLNWLHFEIILFFLVVILMIVVSSFTQAADPAAIR
                     GLYIGSASAEEKAVTRASWNNWDLLASGVIIAVIIAFYAYFW"
     gene            11887..>12066
                     /locus_tag="PK288_07760"
     CDS             11887..>12066
                     /locus_tag="PK288_07760"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the phosphorylation of ribulose to
                     ribulose 5-phosphate; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPM18858.1"
                     /translation="MTGKYVIGIDFGTDSVRSVIVDASNGAIAGTSVHEYTMWKKGMF
                     CDPAANRFRQHPADYL"
BASE COUNT         2979 a         2803 c         2981 g         3303 t
ORIGIN      
        1 agtgagttat acttcttaac tgaaatggtg gtatataatt gaactgaaat ttacatttga
       61 ggagggttta aatcagcaac ccatatttca aatctgtgaa ctggcataat ctgtctcttc
      121 aaattcattg agtacggaaa gggactcgtc ttttaccaga gcggattctt tcctgagttt
      181 tgattctaat attttacgtc tctgaatttt attataataa tcaagagcct catttatata
      241 cctgtttctt ggtttcttta tcttggagag aatattctca gtttctccgt agatcgagtc
      301 gtcaattatt aatgatattg ttttcatggc aaaaatatta ttctacaaag atatatacaa
      361 tatgtatata ctgtaagtat gtttaatgaa atttatcaga tacattacgg cattcagcat
      421 aactttttaa atgtttgcta aatacacata aagagttgga tttattgcag ttggaataac
      481 tgtaatgaca aaaagccggc cctgttttta gatcaataat gcatgccggg tgaatcaggg
      541 ttttcagggt gccggccggg cagcatgaat gtggtttaac agtaacagca ttccgttcaa
      601 atgagagttt ttttcttttc gatttcatta tgtactggtt taatgttaac aaatttaacc
      661 ttaaccggtg gtcttaattt tagggagtat tatagtgtcc cggcttcact gttcacttaa
      721 gtatcacctt tatccggatg gtgattttta tctggcaggg gaatgcgggc ccggaatatt
      781 cattttgtgt atcgggttgc atgcctagat aaaaaaaggc agttattttt gtaactgcct
      841 gattgccttg tcggctcgga gggactcgaa ccctcgaccc catgattaag agtcacgtgc
      901 tctaccagct gagctacgaa ccgttgaatt ttcagtttgc aaatttaata aattgtaatt
      961 ggctgcagca tgtattattg aaaaaatatt cctctccggc cttaactgcc ttttaatgtc
     1021 ccatttcatg cctgcttaat gttttgtccg gcagtaatcg gggctgtttt atcgtgccac
     1081 ggcatccgcg aaccatggga gcctgacact atccggcgca tatttacacc cttcatgctg
     1141 acccggtgat ggcggctgct tatttcagtc ccggcctatg ttgtcgaacc atccgtattt
     1201 cccggctgta acgggttcga tgtagctccg gtgattaaat tcgttggtgt ggcggtcgta
     1261 aagatagtcc ttgccccatt ccgcggcatt taatgcaatc tcttccaccg cggccgcaat
     1321 ataaagcagc tcactgtcgg tcatcagggg gtgaagcgac aacctgaccc atccgggttt
     1381 taatgacagg tcgccggctt ctatcctgtc agtgatttcc ctcgaaagat ctatatcaac
     1441 ctccaggaga aaatggccgt aggtgccggc gcatgagcat cctcccctta cctggatgcc
     1501 atacctgtca ttcagcagct ttgtgatgag attatggtgg acaccgtcaa tatagaatga
     1561 aaaaaccccg agccggtcgg ttatgttttc cgcgaggatg tgcagtcctt ttattgctga
     1621 cagtttttta aatgcaagtt ccctgagttc cctttccctg gcctgtatct tttcagtcgt
     1681 catctcctcc ttgagcctga ttgcaagtgc cgccctgatg ccctgcagga atcccggggt
     1741 tccgccatct tccttgactt caatatcgct tatgtaactg tatccgcccc agcggttggt
     1801 ccatttgaca gtccctccgc cgggagtgtc cggcgtttta ttcttgtaaa gctgcctgtt
     1861 gaaaaccagc acacccgcac tgcccggacc gccgatgaat ttatgggggg agaagaaaat
     1921 ggcatcaagc tggtgcagcc cgttccccgg gtgcatgttt atttcagtat agggagcgga
     1981 ggcggcaaaa tcaacaaagc aatagccgtt gtgctgatgc atgatcgcgg caagctcgta
     2041 atagggtgga atgtatcctg tgacgtttga acatgcggtg aatgatccga tcagcaaggg
     2101 tcgatctctg tacctggcga tctccctcct gagaacgtca gggtttacgg taaggtctgc
     2161 agttggctcc agcacaacca cgtcggcaag tgtttcgaac caggatgtgt gattggaatg
     2221 atgttccatg tgggtaagga acactacagg cctgttctca tttggtacct ccctgcagtt
     2281 gttgtattca ccgtggttga aaacgcagta gtttattgct tgctctggca ccttgagccc
     2341 gagtatcctc tgaagctttg ctatggccga cgtcatgccc gtcccggtga agatcagcac
     2401 atcattctca tcagcgttta catgatgttt tacgatcttc tgcgccatgt gataggcatc
     2461 cgtcattgct ttccctgtag ccgtcgattc tgaatgtgta ttgcccacca tgggcccgat
     2521 ctcattgatt atccgctttt ctatggggcc gtacaacctt ccgctggcta tccagtcggc
     2581 atagaccagc ttcctggttc cgtatggggt ttcaatacag gcatcaatgc caatgacatt
     2641 tttcctgaat ggttcaaagt atttttcaag atctgacatc ggggaagtga attgatgatt
     2701 tttccggatc agtaaaaata tcaaatattt gtatattgcg ccaataagct ttttgataat
     2761 cagtgtaatg aacaatcatt tctgttttat gacagatctt tatcattttc tggtcccctt
     2821 tgttttcata atgtcaggag tggtactggt catcctgctt ttcatttccg ccccttacgg
     2881 gaagtttctc cgcaggggct ggggcccggt tatcaggtca aaatgggcat ggatggtgat
     2941 ggagtttccc tcgccggcac tgatgatact gttttttgta acttctgccg atcacggccc
     3001 tgtggcaggc gtctttctct ccatctggct gctgcattat cttcaccgaa cctttgttta
     3061 ccctttccgc cagtcgggca gggataagga ttttcctgca tccctggttg taatggcttt
     3121 agtcttcaac atcatcaacg gcacgataaa cggttacggg atatttcatc tccgctcgta
     3181 cgacgtgtca tatctggccg gcggcagatt catttgcggg gcggtccttt tcgttacggg
     3241 atttattata aacaaatggg ccgatgagaa attgcgcagg ctcaggagcc ggaaccccga
     3301 tgaatatgca cttccttcgg gagggctttt tgactatata tcgaaccctc attatttcgg
     3361 tgagatcctt gagtgggccg gctgggcaac agctacctgg tcgctgcccg gactggcatt
     3421 tttcctgttc acatttgcca accttttccc cagggccatt gcttcccaca aatggtacag
     3481 ggaacatttc agcgattttc ccggaagcag gaaagctctt atccctttta tagtataagg
     3541 aaactcattc ccgcccgcca gccccggctg cacccggtct tccggaggct gccggaccgg
     3601 tatgccgtat catgtcatgg ttaaccctgg tccgcattca tttatggtcg gatcagtatg
     3661 tcagctgcta cacgggcccc tgttatcggc accggaagta cctgtcggtg aaacgttcaa
     3721 aaattatcaa tatccgtatt aaaattatgg aaaaaccttt tggaaagtaa ttaaatgaaa
     3781 tttacttttg cgcccgaaat aaatttctca taataaaatg gacgacggcc ctgcgaatat
     3841 atggtttaga aatcgggtat aggggaggct gactgttcac cggattgctt ccttcgtatc
     3901 caaagaaaag gaggcaatta tgacttcact cacaacattt tatttaagcg gaataaccgg
     3961 taaggaagtt tttgatgccg acggcgaagc aattggacgc atccgcgatc tgctcatcag
     4021 caatgctttt tccggaacgg gcgattcgaa ccagcagcgg gtgattggaa tcaggcttaa
     4081 agtccggaag gatataagat attattcgtt tgataccttc agggtggtga aggcacgtga
     4141 aatgatcagc gtaacctgtt cagggctcat tgaactgagc gaagagacgg tgaacaacgg
     4201 gctcctgctg gtggaaaaca tgctcgacag gcagattgtg gatcttaacg gccgcaagct
     4261 ggtaagggtc aatgatgtaa ggtttgtgac ccttccggcc ggtacctttg cggttgccgt
     4321 cgatatcggt atcgaaggcc tgctcaggag actcggcatt tcaatcccca ttaaaaggtt
     4381 cctgtccctt ttcaatataa ggataccggc gaaattcata ctctgggatg acgttcaggc
     4441 gatagatttt tcaaaccgca acataaaact ttcaaaaagc tacgcgaagc tgcacatgct
     4501 tcatccttcc gatcttgcag acattctgga ggacctgggt aaaaaaacca gcccccatgt
     4561 cttttctgcc ctcgatgagg aaaaagccgc cgatgttctg gaagaacttg aaccccatgc
     4621 ccagatccac attattgaaa gcctgcccgt tccgaaggtt gcagatgttc ttgaaaagat
     4681 gccggctgat gaggttgcat acattctcga tgagattgag gatgaaaagg ctgaactcct
     4741 cctgaaggag atggacagtg agtcgtcaca ggaagtcagg gagctccttg aataccccga
     4801 caactcggca ggaggtctga tgacaaccga cgttctttcc ttcagtcctt tgctcactgt
     4861 tgatgaggta atcagggagc tgaggatcaa aaagcccgaa ccttcggaac tgtattctct
     4921 ttttgtaacc gaaaggaatg aggagctgat cggtaccttt gatctgcgcg accttgttgt
     4981 tgccgatcct gaaacgataa taaggaatat catgaaaacc gaacccgtgt ctcttttcga
     5041 ttatcagcgt attgatgata tcgccgaact gatttcgaaa tataacctgc ttgcagttcc
     5101 cgttgtggat aaacggaacc agttgcaggg aatggtcgtt gttgatgatg tcatcgagga
     5161 tctgataagc cggagaagga ccaacaagcg ataaaacaaa cggcatgttc aggacaaaat
     5221 tcagcaagac aaggttctat cgtaacctga ctatcttcgc cgctctgctg ggacccggaa
     5281 taattaccgg gagtgttgac aatgatgccg ggggcataac aacatattcc gttgcaggag
     5341 ccatttatgg ctatggaatg gtatggacac ttataccttc actgatagta ttgctggtca
     5401 tccaggagat gaatgcacgt atgggaattg tgaccggcaa gggactttcc gatctgatcc
     5461 gcgaaaatgc cggagttaag atcaccttct ttattttcat cggtttgctg ctgtcaaata
     5521 tcggaaacac caccaccgag tttgcaggtg tcgccgggag catggagata ttcggcatga
     5581 gcaaatatat ttcggtaccc ctggtagctt ttatggtatg gatccttgtg gtaaaaggta
     5641 cctataaggt cgctgaaagg attttcctgg ttttcagcgc ttcattgctt acatacgtgg
     5701 tttcggccct gatgggcgag ccgcgctgga gcgagatcgg ttcagccata atcagacccg
     5761 gaataatgct taacactcaa agcgttacgg tcatcatagc ccttgtggga accaccatag
     5821 ccccgtggat gcagttttac atgcagtcgt cggtcattga gaaaggactc aagataaagg
     5881 actacaaata taccgtgatt gacatagttg tcggctgcat tgtgacggtt gtggttgctt
     5941 tttttattat gatagcctgc gggtcaacgc tgcatccgaa tgggatacgg ataaacgaag
     6001 caaaggacgc ggcccttgca cttaaacccc ttgcaggtga acttgcaagc catgtttttg
     6061 ctttcggcct gtttgttgca tccgtctttt ctgctacgat actgccgctg gcaacctcct
     6121 tttacatatg tgaggcattt ggttttgaaa ccggactgaa caaatcatgg agcgaagcca
     6181 gggaatttta tgttctgtat acaggaatac tggtcgtttc ggctgtcatt atcctgatcc
     6241 ccaatgcacc gttaatagat atatccatct ggtcgcaggt tctcaatggc atcctgctgc
     6301 ccgttgtgct tgtatgcatg atcttgctgg tcaatgacaa aaagatcatg ggtgaatata
     6361 caaataactc cctgcagaac ataataggat ggggggcggt agtcattatg accctgctgt
     6421 cgctgacact cctggtaata ccttttttcc ccggccggta aggctatctg cggggccagg
     6481 cgccattaat catgggcctg ggttcgatcc tggccggatc aacaacaacg tgcctgataa
     6541 gcctgcgtct ccatgtgtag gttatgtgga tcagtccgtc agcggattga attacggcag
     6601 gatacgaata ttcctttccc tctgaatcgt cttccagcag caaggctgcc ttccatgttt
     6661 ttccatcatc ggaaagtgca gcatttagct gatgcctgcc tttggtaagg tgattatata
     6721 caaggaaatg ccttccatcg gtcagggtca cggcatcaat ccccgaattg ggattgggca
     6781 gggaatcggg catcagcctg ctccagctat ttcccttgtc gtccgaccat gatgaaaaaa
     6841 tccgcgaccg gtttgtgcgg cacaggatct ggagcctgcc tgttttatgg gtcagaatgg
     6901 ctggctgtat cgcatatagg tccttgttat tgaggaaacc ggttctttcc catgttatgc
     6961 caaaatcagg agtgaactcc atgtgaaccc gccatccgtc atgttcggtg ctcgaagggc
     7021 aaaggagctt tccattatcc agcagtacag gtttgttttt cactgggccc agaatgtcgt
     7081 caggaagctt ccggggagca gaccaggaaa tgccattgtc cgtggaaatc ttcagctccc
     7141 cccaccattc ccggggattt ggtcctactt tatagaagag cagtatttca tcatcaccgt
     7201 tatacagaac cgggttccag cagggatatc tgagactgtc ggttttcccg tcagcaacca
     7261 tttccgggat cgaccatcgc ccgccggtaa atctgcttat ccagatcccg acatcgggat
     7321 tgccttcagc agtaccgccg aaccatgcgg caatgaaacc gcccggcatt tcggctatcg
     7381 ttgaggcatg acaacccgga aaccagaccg aggacgaatc atatatgaat tcgctcctga
     7441 caatgcactg atcacccctg aagggatcct gggcacaaag aaaatcattg acagacagga
     7501 atatcagtat tccggtcagg aattttatcg ataaaccggt tagctttttc ctttccttca
     7561 ccatggcaaa attgtttttt ttatactgac cttttgaatt aatcatttga aatgatttct
     7621 gaaccgcatt ttcaatatca ggaatgtttt ttccgggctc tgtaactgct gatcagaaag
     7681 ccggtaagga aaataacaat tgttccgaaa acgattgtca ggttgctgtg gaccggattt
     7741 ctgaatgaag caagcctgcc gttatccgga agcagcggag aaaggctcat ccagacaatt
     7801 acaattatgc cggctgttac accaataaca gcttcagctt tcccggcttt taccgggagg
     7861 aagccgagga gaaacaggcc aagcattccc ccgctgaaga ttgaagccag cgcccaccag
     7921 gcgtccaata cgctttccac gccggtaagc gccagtgcaa tagctgtccc ggcaaagccg
     7981 gtggcagccg tagccgcata aagaatcctc atggatgctt tttcggctgg gtcgcgcttt
     8041 gcaaatttcc tgaaataatc agtaagcacg atcgtggcag tgctgttgag actggtggat
     8101 atggtactca tcccggcagc aaagatggaa gctatcagca aaccggtcag tcctgcagga
     8161 agctcgtgta ctatgaagta gggaaagacc ctgtcgccgg ctgagaccgg ccggagctcc
     8221 tctgccagcc tcccgggctg tgctgtgtaa aatgcaaaca atgccgtgcc tatatagaaa
     8281 aacaacatcg aaacaggcag gtacagcaat gatccgaaaa gagccgattt cttggcttcc
     8341 ctgtcggagc cggttgtcat atacctttga atataattct ggtcgatgct gtagttctgc
     8401 aggtttatga ataacccata cacaagtaca acccagaatg tcgattcgga aaggcttgtg
     8461 ccgaaacttc ccagactgaa tttcccgttc gatgctccta tgctgaaaac ctgcccggct
     8521 ccccccggca tcgacagggt aatatatacg gcgcagatga gggctccggt aattaatata
     8581 aaagcctgga tcgtatcggt ccacactacc gccctaatgc ctccaagcat ggaatacaga
     8641 atgaccgaca gcccggtaag gataattatt gcctttatgt tccatccgaa cagggcattg
     8701 aggggcaggg cgagaagaag caggatcgcc ccggttctca tcagctgggt aagtacatag
     8761 caggaggaag catatatcct ggcccatgac ccgaaacgca tctcgaggaa gtagtatgcc
     8821 gacggatttc ctatcttcct gtaatgcgga acaaaatacc tgaccgcggc aaatgatgca
     8881 acaggtatgg agaggctgaa aacaaaagcg ttccagtcag tcatgtatgc ctttccggga
     8941 agggccagaa aactgatgct gctcaggtat gtcgcaaaaa tcgacatccc ggccacccat
     9001 gccggaatat ttcccccggc ggtaacaaac tggtcggagg atctgctcct gaaataaaaa
     9061 ctcacaccga taattacatt gctgagaacc agtataataa atatgacggc atcaacaggg
     9121 gaaagcttca tgtgacccta ttttgaaata cgcaccttga tgaccgtcta aatttacaat
     9181 ttaatattag atttgtatgt gtattgaaaa acctgtaaaa caagacacct ttcataaacg
     9241 tatgaaatac ggcttttttg attcatggtc atatgataca cccgataaag gcaaaccggg
     9301 cctgtgctac aggatcatcc atcacaacag gttgtatttc accctgaaat atgccaccat
     9361 agtcctcaga acaagaaaag cggccattaa gggtgtttat gacaggaaag catgggcaga
     9421 atcatcttat tatattttca ggttcattga gaaggccggc gggaagtttc acgtctcagg
     9481 gatggaaaat attgtggcgt gtccttctcc agtcgtgttc atcggaaatc acatgagtac
     9541 gctcgagacc atgatccttc cgggactgat cgcaccccat cgtgaagtga cattcgtggt
     9601 aaaggaagga cttgtcaggc atccgctgtt cgggcatgtt atgcgttcgc gcgatcctgt
     9661 tgttgtagga cgaaccgacc cgcggaagga tttcgaagag gtgatgacca aggggcagga
     9721 gcttttatcc aggggtgttt caattgtcat attcccgcaa agtacccgga gtgtggagtt
     9781 cagacctgag gaattcaatt cacttggcgt gaaactggca aaaaaagcag gggttaatgt
     9841 ggtcccggtc gcgttgaaaa ccgacttctg gggacagggg aagatcatta aggaacttgg
     9901 ccccctcgac agttccagac ctatcttttt caggtttggg gaaccatttg cagtaaccgg
     9961 gagcggccgg gaagaaaatc agcgggtcat tgattttgtc cagtcatcgc tggctgaatg
    10021 ggaaaaatga tgccgtgctt tgaaatgtat gccccggaac ccaattactg gctgtaacag
    10081 gtaacttttg ttatttcaat tcttcatatt tattaattct gagcatggaa acacttgact
    10141 ggatcgtcct cgggctgttt tgcctcggcc tgattggcat aatcatctgg gttaccagga
    10201 agaaagatga gacctcaacc gactatttcc tcgccggccg cgatgcaaca tggctcgcga
    10261 tcggatcctc gatctttgcc tccaacatag gctcagagca tctcgttggt cttgcagggg
    10321 caggggcatc gagcgggatg gcgatggccc actgggaaat gcacggatgg ctcatcctga
    10381 tactgggctg gttctttgtt cctttttatg ccagaagcgg agtgttcacc atgccggaat
    10441 tccttgaaaa acgttataca aaaggatcga ggagcgtcct gtcggttatt tccctcgtca
    10501 gttatgtctt cacaaaagtt gccgttacgg tttatgcagg aggaatcgtt ttcaagcagg
    10561 tcttcggcat tgagtcgatg ttcgggatcg attttttctg gataagcgcg gtgggcctgg
    10621 ttgtgctgac aggtatttac accaccctgg ggggaatgaa agccgtgctc tggacctccg
    10681 tcctgcagac acccatcctt ctgatcggtt caataacggt tctgatagtc ggacttgtta
    10741 aactcggagg ctggtcgcag atgatggaaa tctgcagggc ggtcccggtt aatgctgacg
    10801 gcgacaccat gaccagcctg atacggtcgg caaacgatcc tgaatacccg tggacgggag
    10861 tgatactggg atcagccata atcggtttct ggtactggtg caccgaccag tatatcgtcc
    10921 agagggttct gtccggacgg aatcagaccg aatcaaggag aggggccata ctgggtgcag
    10981 cattcaaact gtcgcctgtg tttatcttcc tcattcccgg tatgattgcc tatgcgctaa
    11041 actacagggg catgatcagc ctgcctgatt ccgattcggc attctctgtt cttgtcagcg
    11101 aactgcttcc cctgggattc aagggactag tggtgggcgg aatcctggcg gcactgatga
    11161 gctcccttgc ttcacttttt aattcgtcgg caaccctttt cacggttgac ttctatgaga
    11221 aattcaggcc tcaggcaacg gaaaaggagc tggtaagggt aggaaggatg gcgactgttg
    11281 cggtcgtcat ccttggaata ctttggatac cagtgatgaa gggactgggc aaggtccttt
    11341 atgcttatct ccaggatgtc cagtcactcc tggccccggg aatagcagca gtgttcctgt
    11401 tgggaatagc atccaggaag atcaccccgg cagccggatt cacgggcctt cttacgggat
    11461 ttgtactggg aatgaccagg ctgggattca aggtattcgg aacaggcctg ggtactgaca
    11521 gcatcctgta tacagtgttc ctgaaactta actggctgca ttttgagatt atccttttct
    11581 tccttgtggt aatactgatg attgtggtaa gctcattcac tcaggctgca gaccccgctg
    11641 ccatcagggg tttgtacata ggatcggcct cggcggagga aaaagccgtc acgagagcca
    11701 gctggaacaa ctgggatctt cttgcatcgg gagtgattat tgccgttatc atagcatttt
    11761 atgcttattt ctggtgacag attcacaccg gaggtaatat gcaaggttta ctgttttgtg
    11821 attaacattg attcattcat aactgataat tccttcgaaa aataatcatg atttaataag
    11881 ttggatatga ccggaaaata tgttattgga attgattttg gcactgactc ggtacgatcg
    11941 gtgattgtcg atgcatcgaa cggagcaatt gccgggacct cggttcatga gtacacgatg
    12001 tggaaaaagg ggatgttctg cgatcccgct gccaaccggt tcaggcagca tcctgccgat
    12061 tacctt
//
LOCUS       DAOOYG010000079        11738 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_5773_length_11738_cov_6.241462, whole genome shotgun sequence.
ACCESSION   DAOOYG010000079 DAOOYG010000000
VERSION     DAOOYG010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11738
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5773_length_11738_cov_6.241462"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PK288_07765"
     CDS             <1..342
                     /locus_tag="PK288_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPM18859.1"
                     /translation="PGERPSAYIIILGDKTITESFNADPGIAAQSMMLGATEAGLGGC
                     MLTSVKREVLRKEINIPDHYEILIVLALGKPVEKVVIEEVKDGNIKYWRDSDQTHHVP
                     KRKLEDVVIIV"
     gene            356..1072
                     /locus_tag="PK288_07770"
     CDS             356..1072
                     /locus_tag="PK288_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18860.1"
                     /translation="MNRIVIILLSFIFLSPVEAQYANNEQQRVRLQMIVRDAESFSPL
                     ENSQVFINRVFYGITDSTGTFSVLLGVNDTVECRLLGYRPALFVLSDSLSGRDFMAGI
                     YLNTDTVRIGEVIIVPRLVNLKSDIFRAPEPVSREMENARANLALSAYQAKASQGKLG
                     DPLTNYEVIKMQQRTQAYEKGQIPSSAVVAVSPFMIVPALYLLMNGLPPGPMPMKPDI
                     TEEELLQIHKKYLELQGTRK"
     gene            complement(1121..2032)
                     /locus_tag="PK288_07775"
     CDS             complement(1121..2032)
                     /locus_tag="PK288_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPM18861.1"
                     /translation="MLNKLIASLLPFFPKKFIWLFSRSYISGETIEDAMRVANELNSK
                     QIKATIDVLGEFITTLDEAEANKREYLNLIEVASRAGIDANFSIKPTSFGLLLDKNEC
                     QKHVREIIAKAASYNNFIRIDMEDSSCTDLEIELFRKMVTEFPGKVGLVVQAYLKRTL
                     NDIRELSDLNSGEYPLSFRLCKGIYVEHESIAYKKYEEINQHFLEDLEYMLKNNIHVG
                     IATHDKPLIEGAIDLVRKYDVPRKMYEFQMLYGVTPRLRDSIASDGHTMRVYVPFGKE
                     WFGYSTRRLKENPKMASHIIKAIFYKG"
     gene            2376..3362
                     /locus_tag="PK288_07780"
     CDS             2376..3362
                     /locus_tag="PK288_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPM18862.1"
                     /translation="MLKKFKYTFLLPLIIFADGYCQDPTFSQFYANPLYLAPSFAGAT
                     EEYRIALNYRNQWPAIPGVFHTYSISFDKALVNYNSGIGIQATYDVAGSGDLSTTNIG
                     LLYSYDFNINEDWHIRPGVNFKFRYLGLNIYKLVFASQMTGSGNVPSVYPPPFDKVAD
                     IDFAASALVYNDRIWGGFTLDHLLTPKQSFYGDDALLPIKLNVFGGIQIMRKTRLKQT
                     FHEVLSAAVNFQKQDKFYQTDLGLYYYRDPLIFGLWYRGIPFVTTQAGDAIIGLVGLK
                     TKQLHIGYSYDFTISNLITSTSGSHEISLVYEFNSFTLGQRKKIRAIPCPEF"
     gene            3525..4121
                     /locus_tag="PK288_07785"
     CDS             3525..4121
                     /locus_tag="PK288_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPM18863.1"
                     /translation="MKKLYVILFSILFLAVAALFVLHFTGGSKSGNNAGGGEAATSPQ
                     GIAFVNIDTIVFNFKMFEDRRSDLIEKQKKAEAELNTRGNQYERGIKDFQNKVSKGLI
                     TRAAAAEMEQALIQQQQELVNLRDRLQADLVEEDQVMNRQILEYITTFLEENKSEYNY
                     DYIFGKSFGSVVLYGNSGHDITRKVLDALNAKYKAEKK"
     gene            4128..5393
                     /locus_tag="PK288_07790"
     CDS             4128..5393
                     /locus_tag="PK288_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18864.1"
                     /translation="MGFASTYLKERTLYPKLIGEAPHRDTGIIVVVPSCNEPSITNLL
                     DSLASCAEPACATEVIIVVNSPAGAGPDVRNANIATLNETAEWKIRNPRSFLKLYTID
                     ADTSQFCKWGVGMARKAGMDEALRRFDELERPDGIILNIDADCTVENNYFTEVYGGIP
                     AGGNRSACSIYFEHPLEGKEFPQAVYRAIAQYELHLRYYYQGLCFAGFPNVHHTVGSA
                     IAVKAMAYLKSGGMNRRMAGEDFYFIQKLVSAGGYFNLNSTTVYPSPRPSARVPFGTG
                     PTIFRLAESNSGELMTYNTDAFRELRAFFCMTDSFYNTSNNENHYLYNELPESVRLFI
                     EIEEWNRAICEIKGNTASLKSFRKRFYDWFNMFRIVKYMNFAHEDIMKKKPVVESALE
                     LLNESGHDFYSGDPVALLNFYRSLEKGYL"
     gene            complement(5390..5728)
                     /locus_tag="PK288_07795"
     CDS             complement(5390..5728)
                     /locus_tag="PK288_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18865.1"
                     /translation="MVRFRFIEKIPPVLRNKYILTLVIFVIWLLLLDSNNLIARYREL
                     KNLRRLRAEKEYYTRRIEEDRSKLQELRTDNRNLEKFAREQYKMKKPDEDLYIVLSPR
                     EDRQIKRKHN"
     gene            complement(5895..7187)
                     /locus_tag="PK288_07800"
     CDS             complement(5895..7187)
                     /locus_tag="PK288_07800"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HPM18866.1"
                     /translation="MRKFSLLFFIFILPGLCTLVSAQNEEKILAGTIFSNSVKTVRLY
                     REEWNLSYPVISIGGNERLVLQFDLLGDISETFYYTFIHCDKDWNRSGIFTTDFIDGF
                     PENPVEDYHPSFNTTIHYTNYRLAFPNERVNLKLSGNYIIMIFPSGEPENPVLTQRFI
                     VSENLVGITVEAHRPGMTAYYNENQQIDFTVDYSGIKINDPYQDVFASILQNGRWNNA
                     KRNIKPEFYGNNKLKYSALSDKNIFAGGNEFRYFDIRSIRYQSEYVKKIDFITSNYHV
                     LLTPSENREFKPYFYSQDFNGKYYVAIQEENNPDTEADYVYVYFTLPSASKVEGGKMY
                     VSGALSNWSYGPDNLMSYNIETRAYECILLLKQGWYNYEYFFQQDGDESGVTSKFEGN
                     HYETENDYTVIIYYRNPRDRFDRVIGTQTVNTLNRIIY"
     gene            7397..8749
                     /locus_tag="PK288_07805"
     CDS             7397..8749
                     /locus_tag="PK288_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HPM18867.1"
                     /translation="MPRTIKLKKGFDIRLKGVAGKKMAGSAEPDLFGVKPIDFPGLTP
                     KLNVKEGSEVLAGAILFHDKHRPEIIYTSPVNGRVVSIERGERRKILEVVIEKRGNEA
                     IDFGKTRHEEITEERVKESLLASGLWPIIRQRPYHIVANPHDIPKSVFVTGFDSSPLS
                     PDYDFILENSNISHYRTGLYALSKLANGRINLVLKEKSGAGSYFSDIPGIEIHYFSGP
                     HPSGNPGIHIHHIDPVNKGEVVWFANLQDVSILGRLLEEGIYKPEKIVALTGSEVINP
                     QYYRILSGGSVASFVADNVRPGNNRYISGNVLTGTRIRSDGYLGYYDSQVTVIPEGNY
                     HEFFGWATPGIGKFSFSRTFLSSLFPRKEYRLDTNLHGGERAFVVTGQYEKVLPMDIY
                     PMQLLKAILAEDIEMMENLGIYEVAEEDLALCEYLCPSKIEIQSIVRKGIDLMIKEMS
                     "
     gene            8771..9946
                     /locus_tag="PK288_07810"
     CDS             8771..9946
                     /locus_tag="PK288_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HPM18868.1"
                     /translation="MNSLRKTLDKLKPHFSKGGRFSKLQSVFDGMESFMFVPDKVTFS
                     GSHIRDYTDLKRTMTIVIIALLPALLFGSYNTGLQHFRSVGIESGIFQSFGYGFLRIL
                     PFIIVSYLVGLGIEFIAAQIRGHEINEGYLVTGLLIPLIMPIDVPLWMLALAVAFAVI
                     LGKEVFGGTGMNTFNPALLARAFVFFSYPGWMTGDTVWTAGLTAGKNIADGFSGATPL
                     SLCVTGEISRLPSFMEMFVGTIPGSFGETSVIAILIGAAVLIATGVGSWKIMAGVFGG
                     GLAMGLLLNAISANEYMHLPFYYQFVMGGFAFGAVFMATDPVTAAQTEKGKWIYGFLI
                     GVLSILIRVLNPAYPEGVMLAILLMNVFAPLIDYFVIQSSINRRLKRVKVEIKTSVK"
     gene            9943..10644
                     /gene="nqrC"
                     /locus_tag="PK288_07815"
     CDS             9943..10644
                     /gene="nqrC"
                     /locus_tag="PK288_07815"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HPM18869.1"
                     /translation="MKQFSNRYIFIFSSVMVIAVAALLSLAATLLQPAQTRNLEIEKK
                     RNMLESINVAATRENTADLYGKYIKEGFVINSRGEMIEGVDAFSVVLRNEQKKPLAEQ
                     YLPVFRAVPDDGEQVVILPVEGKGLWGPIFGYISLRSDMNTIYGVNFDHKGETPGLGA
                     EINTSAFESQFRGKKLYDNTDFISVHVVKGGAREGDVHGVDAISGGTITSKGLEMMIH
                     DCMLKYNDFLLKNRN"
     gene            10657..11301
                     /locus_tag="PK288_07820"
     CDS             10657..11301
                     /locus_tag="PK288_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HPM18870.1"
                     /translation="MEKESIFSPRNLKLLTEPLGKNNPITVQVLGICSALAVTVKLKP
                     ALVMSLSVIIVTGMSNLVIAAFRKYIPETIRIIVQLVVIATLVILVDQFLKAYVFDVS
                     KQLSVFVGLIITNCIIMGRLEAFAMANKPWPSFLDGIGNGAGYGLILVVVAFFRELLG
                     SGTVYGYPVLEKLGLFKTGYENNGMVILPPMALIFVGLIIWFQRSRDKSLVEKN"
     gene            11312..>11738
                     /locus_tag="PK288_07825"
     CDS             11312..>11738
                     /locus_tag="PK288_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPM18871.1"
                     /translation="MENLLNIFIKSIFVDNMIFAFFLGMCSYLAVSKNVKTAIGLGMA
                     VVFVMFITVPVDYLIEKYVLKKGALSWLGEGFADVDLSFLTFMVFIAVIAAIVQLVEM
                     VVEKFSPALYNSLGIFLPLIAVNCAILGGSLFMQEREYAN"
BASE COUNT         3189 a         2387 c         2752 g         3410 t
ORIGIN      
        1 cccggggaaa gaccttcggc ttacataata atactgggcg acaagacaat taccgaatca
       61 ttcaatgccg atccggggat tgcagcgcaa agtatgatgc ttggagctac ggaggccggt
      121 cttggcggct gcatgctgac ttcagtaaaa agggaggtgt tgagaaagga aatcaatata
      181 cctgaccatt atgagatact gattgtgctt gctctcggaa agcctgtcga aaaagtggtt
      241 attgaggaag ttaaggatgg taatataaaa tactggcgtg acagcgatca gacacatcat
      301 gttccgaaaa ggaaacttga agatgtggta attatagttt aattttggtt atgccatgaa
      361 caggatagtg attatattac tgtcgttcat cttcctgagt cctgttgaag cccagtacgc
      421 aaacaatgag cagcagaggg ttcgtctgca gatgattgtg cgggatgctg aatccttctc
      481 gcctctggag aattcccagg ttttcatcaa cagggtattt tatggtatca ctgattccac
      541 gggcactttt tcggtacttc tgggggtgaa tgataccgtt gagtgcagac tgctcggtta
      601 caggccggcc ttgtttgtat taagcgattc cctttcgggg cgagatttca tggccggtat
      661 ctatctcaac accgataccg tgaggattgg cgaggtaata attgtcccaa ggcttgtgaa
      721 tctgaaatca gatatcttca gggcaccgga gcctgttagc cgcgagatgg agaacgcaag
      781 ggctaatctt gccctgtcag catatcaggc aaaagcaagt cagggaaaac ttggcgatcc
      841 cctgaccaat tatgaggtta tcaaaatgca acagcgtacc caggcctatg agaaaggtca
      901 gataccttca tcggctgttg ttgctgtgag ccccttcatg attgttccag cattatatct
      961 tttaatgaac gggctaccac cgggacccat gccaatgaaa cccgatatca ccgaagagga
     1021 attactccag atacacaaaa agtaccttga attgcagggg acgaggaaat gatatggcta
     1081 tttcctgcca tgatccgtac ctaatgcagg atatgcctta ttaaccttta tagaagatcg
     1141 ctttgataat gtgacttgcc attttcggat tttccttgag ccttctggtt gagtagccga
     1201 accattcctt tccgaatggg acataaaccc gcatggtatg gccatcgctt gcaatactgt
     1261 cgcgcaggcg cggtgtaact ccgtatagca tctggaactc atacattttg cggggaacat
     1321 cgtactttct gacaagatcg atggcacctt cgatcagagg tttatcatgg gtggcaatac
     1381 cgacatgaat attgttttta agcatatact caagatcctc aaggaaatgc tgatttatct
     1441 cttcatactt tttataagcg atcgattcat gttcaacata gatacctttg caaagcctga
     1501 aactcagggg gtattcacct gagttcagat ccgacagttc cctaatgtca ttgagcgttc
     1561 ttttcagata tgcctgaacg acgagtccca ccttgccggg gaattcggtc accattttgc
     1621 ggaatagttc aatttccagg tctgtgcatg acgaatcttc catgtcgatc ctgataaaat
     1681 tgttgtaaga agctgcttta gcaattattt cacgaacatg tttctggcac tcgtttttat
     1741 ccagaagcag tccgaagctt gtgggtttaa ttgaaaaatt cgcatcaata ccggcacgcg
     1801 atgcaacctc aatcaggtta agatactccc ttttgttggc ttcggcttcg tccagggttg
     1861 taataaattc ccctagaaca tcaatggtgg ctttaatctg cttgctgttt agttcattgg
     1921 ccaccctcat cgcatcctct atcgtttcac ctgaaatata agatcttgaa aagagccaga
     1981 taaacttttt aggaaagaat ggcagcaggg aagctataag tttgttcagc attttgatgg
     2041 tccggttaat aagtgatgaa aggtttcata ttgatgcccc agggaggtaa tcctgaaaac
     2101 aaaatagcaa ataaataaat atggaatata gatataaatg acgtttgtca caagaaagga
     2161 ctgattttac tcccggatct tttatccctg aatgataatt attttttgtt attatcctga
     2221 ttattatgcc ggcggcagtc aataaataat aaatttaata accccggaaa taaaaatctt
     2281 gtctgacagt gtaccttttt caggaaaatt gcgtttatca actgatgata caaagtgtta
     2341 attagtttag gttacacaaa acaaacggta cgggtttgct taaaaagttt aaatatacgt
     2401 tcttattgcc gctgatcata tttgccgatg gatattgcca ggatccgacc ttttcccagt
     2461 tctatgcaaa tcctctttat cttgcaccgt catttgccgg agccacggag gagtacagga
     2521 ttgccctgaa ttacaggaac cagtggcctg caattcccgg ggtgtttcat acctacagta
     2581 tttcattcga caaggcgctt gtgaattaca attcgggtat tggaattcag gcaacatacg
     2641 acgttgcagg atcgggcgat cttagcacaa caaatattgg cctgctgtat tcatatgatt
     2701 tcaacataaa tgaagactgg catatcaggc cgggcgtgaa ttttaaattc aggtatctgg
     2761 gcctgaatat ctacaaactt gtatttgcga gccagatgac aggcagcggg aatgttccct
     2821 cagtctatcc gcctcctttt gataaggttg cagacattga ttttgcagca tccgcacttg
     2881 tatataacga cagaatctgg ggaggattta cccttgatca cctgcttacc ccgaaacaat
     2941 cattttatgg tgatgatgct cttctgccaa tcaagctgaa tgtattcggg ggtatccaga
     3001 tcatgagaaa gactagattg aagcagactt ttcatgaggt tctgtcggct gctgtgaatt
     3061 tccagaagca ggacaaattc taccagaccg acctgggatt gtattactac agggatccgc
     3121 tgattttcgg actgtggtac cggggaatcc cgtttgttac cacacaggcc ggggacgcca
     3181 ttatagggct tgtgggcctg aaaacaaaac aacttcatat aggatacagc tacgatttta
     3241 caatttccaa cctgatcact tcaacatcag gatcgcatga gatttccctc gtgtatgaat
     3301 tcaattcctt tacccttggc cagaggaaga agataagggc aattccgtgt cccgaatttt
     3361 agaaatcctt tttaaaaagc aggggaaaca actggtatct ttttactttc tgattgctag
     3421 aaaacattca aaagcagagc gttaaaaata actttgcgga gtgtgccgat agatttattt
     3481 ttttatattt gcgcctgaat taaataatta acaaccaacc tgagatgaag aaattatatg
     3541 taattctgtt cagtatcctt tttttggcgg tcgcggccct gtttgttctt cattttaccg
     3601 gcgggagtaa atccgggaat aatgcgggcg gaggcgaggc tgcaacttcc cctcaaggaa
     3661 ttgcatttgt caatattgac accattgttt tcaacttcaa gatgtttgaa gacaggagat
     3721 ccgatctcat tgaaaagcaa aaaaaggcag aagcagagct taataccagg gggaaccagt
     3781 atgaaagagg gattaaggat tttcagaata aggtaagcaa aggactgata accagggctg
     3841 cagcggcaga aatggagcag gctctgatac agcagcagca ggaactggtc aaccttcgcg
     3901 acaggcttca ggccgacctt gtggaagaag accaggttat gaacaggcag atacttgagt
     3961 atattaccac gtttcttgaa gaaaacaagt cggaatacaa ttacgattat attttcggca
     4021 aaagtttcgg aagtgttgtt ctttacggta acagcggcca tgacataacc aggaaagttc
     4081 tggatgccct gaatgctaaa tacaaggcag aaaagaaata ggatcaaatg ggctttgcct
     4141 cgacctatct gaaagagagg acactttatc caaagcttat cggagaggca ccccaccgcg
     4201 atacagggat cattgtcgtt gttccctcct gtaacgaacc atcaatcaca aacctgctgg
     4261 attccctggc atcatgcgcc gagccggcat gtgcaaccga ggtgataata gttgttaatt
     4321 cgccggccgg tgccggcccc gatgtccgta acgcaaacat agcaacactg aatgagacag
     4381 cggaatggaa aatcagaaat ccgcgatcat ttctgaaact gtatacgata gatgccgaca
     4441 catcacaatt ttgcaagtgg ggggttggga tggcccggaa ggctggaatg gatgaagctt
     4501 tgagacgttt tgacgagttg gaaaggcctg atggaattat acttaacatc gatgccgatt
     4561 gtacggttga gaataactat tttacagagg tttatggcgg aattccagcc ggcggcaata
     4621 gatcggcctg ttccatttat tttgagcatc cgctggaggg aaaggaattt ccacaggcgg
     4681 tatacagggc aattgcccaa tatgaacttc acctgagata ttattaccag ggcttatgtt
     4741 ttgccgggtt tccgaatgta catcatacgg tcggttccgc cattgccgtc aaggcaatgg
     4801 cctatttaaa atcaggagga atgaaccgca ggatggcagg tgaggatttt tattttatcc
     4861 agaagcttgt gtctgccggc ggttatttca acctgaattc cacaacggtt tacccgtcgc
     4921 cgcgcccctc agcaagagtg ccgttcggta ccggaccaac aatcttcaga ctcgcggaaa
     4981 gcaattccgg ggaactcatg acttacaata ccgatgcatt cagggaactg agggcgtttt
     5041 tctgtatgac cgactcattt tacaatacaa gcaataatga aaaccactat ttgtacaacg
     5101 aacttccgga aagtgtgaga ttattcattg agattgaaga atggaatagg gcaatatgtg
     5161 agattaaggg aaacactgca agcctgaagt cgttcagaaa gaggttctac gactggttca
     5221 atatgttcag gattgttaag tatatgaact ttgcccatga agatattatg aaaaagaaac
     5281 ctgttgtgga atcggctctt gagcttttaa atgaatcagg acatgatttt tattcaggcg
     5341 atcccgtggc ccttctgaat ttttacagat cgcttgaaaa aggatatctt tagttatgct
     5401 tcctttttat ctgcctgtct tcacggggag aaaggacaat atacagatcc tcatcaggct
     5461 ttttcatttt atactgctcc ctggcgaatt tttcgaggtt ccggttgtcg gtcctgagtt
     5521 cctgaagctt acttctgtcc tcttcaatcc gtcgggtata gtattccttt tctgccctga
     5581 gcctgcgcag gtttttcaat tccctgtatc tggcgatgag attgtttgaa tcaagaagca
     5641 acagccagat gacaaaaatt acgagcgtaa ggatatattt attccttaaa accgggggta
     5701 tcttctctat aaacctgaac cttaccattg tttatgcaaa tctataactt ttttttatcc
     5761 cgcgctcaaa agatagttca tctgaacatt atctccgttt ttagtatgtg attaatcata
     5821 tttatcccta gcaaaaagtt gtcagttgat tttaacaact ctcaacctga aggtatactt
     5881 tttcatccgg cgcatcaata aatgatccgg ttgagagtgt tgaccgtctg cgtcccgatt
     5941 acccggtcaa atctgtcacg gggattccgg taataaatta ttaccgtgta atcgttttca
     6001 gtttcgtaat gattcccctc gaattttgag gtcacaccgc tttcatcacc gtcctgctga
     6061 aaaaaatact catagttgta ccagccctgt ttcagaagca atatacattc ataggccctg
     6121 gtttcaatgt tgtagctcat cagattatcg ggcccgtagg accagtttga cagtgcaccc
     6181 gaaacataca tttttccgcc ttcaaccttt gaagctgacg gaagggtaaa ataaacatat
     6241 acatagtctg cttcagtgtc gggattattt tcctcctgta tggctacata gtactttccg
     6301 ttgaaatcct ggctgtaaaa atatggtttg aattctctgt tttctgacgg agttagtaaa
     6361 acatggtagt ttgatgttat aaagtcaatt ttcttgacat actccgactg gtatctaatg
     6421 ctccttatat cgaaatatct gaattcgttt cctcccgcga aaatattctt atccgaaagt
     6481 gcactgtatt tcagcttgtt gtttccataa aattcaggtt tgatgtttct ctttgcgttg
     6541 ttccatctcc cgttctggag aatggaggca aaaacatcct gataaggatc attaatttta
     6601 ataccggaat aatccacggt aaaatcaatt tgctggtttt cgttataata tgctgtcatc
     6661 cctggcctgt gagcttcaac agttataccg acaagatttt ccgatacgat gaaacgctgc
     6721 gtgagaaccg ggttctcagg ttctccactg ggaaatatca ttattatata atttcctgac
     6781 agttttaaat ttacacgttc attcggaaag gcgagcctgt aatttgtgta atgtatcgtt
     6841 gtgttgaacg atgggtggta atcctcaacg gggttttccg gaaagccgtc gataaaatcg
     6901 gttgtgaaaa ttccggacct gttccagtcc ttgtcacaat gtataaaggt ataataaaag
     6961 gtctcgctga tatcgccaag caggtcaaac tggagtacca gcctttcatt cccgccgatg
     7021 gatattacgg gatatgaaag attccattct tccctgtata atcttaccgt ttttaccgaa
     7081 ttgctgaaga tggtacctgc gagtattttt tcttcattct gagctgaaac cagtgtgcat
     7141 agaccgggca ggataaaaat gaaaaaaagc aggctgaatt ttctcattgt ttttgtgcaa
     7201 gttttattac aaaattagtg caataatttt atgatttttc acactttctt ttatccatcc
     7261 ctcatgtttg ttaactttgc tgtctgttat tttattaaca gaagacttcc tgacaggatt
     7321 tcttttttat aatttgatct taattgctga aaaagaagtt attgattaac actaatttac
     7381 gatatctgga tctgatatgc cacgtacgat taagctgaaa aaaggatttg acatccggtt
     7441 aaagggtgtt gccgggaaga aaatggccgg cagtgcggag cctgatctgt ttggcgtaaa
     7501 gccaattgat tttcccggtc ttacgcccaa actgaatgtt aaggaaggca gcgaggtact
     7561 tgccggtgcc atcctgtttc atgataagca caggcccgag ataatttaca cctcaccagt
     7621 caatggcagg gtggtttcta ttgagagggg ggagaggaga aagatcctgg aagtggttat
     7681 cgagaaacgg ggaaatgaag caatcgattt tggaaaaacc cgtcatgagg agatcacgga
     7741 agaaagggtt aaagaatcac ttctggcatc gggactgtgg cccattatac gacagaggcc
     7801 gtatcatata gtggccaatc cgcatgacat tcccaaatcg gtttttgtaa ccggatttga
     7861 ttcatcaccg ctgagccctg attatgattt tattctcgag aattcaaata tttctcatta
     7921 caggactgga ttgtatgctc tttcaaagct cgccaatggc aggatcaatc tcgtacttaa
     7981 ggaaaaatct ggtgcaggca gttacttttc tgatatcccg ggcattgaaa ttcattactt
     8041 ttcaggaccg catccttccg gaaatcctgg tattcatatt caccacatcg atcctgtcaa
     8101 caagggtgaa gtggtgtggt ttgccaatct gcaggatgtt tcaatactgg gaaggctgct
     8161 ggaggaaggg atatacaagc cggaaaagat agtggcatta acaggatcgg aggttattaa
     8221 cccacaatac tacaggatcc tgtccggggg gtcggtggct tcatttgtgg cggataatgt
     8281 caggcccggc aataatcgtt acataagcgg caatgtgctt accgggacca ggatcaggtc
     8341 tgacggatat cttggatatt atgattctca ggttacagtt attccggaag ggaattatca
     8401 tgagttcttc ggttgggcaa cacccggtat cggaaaattc agcttttccc ggactttcct
     8461 gtcgagtctt ttcccgagga aggaataccg gctcgacacc aatctgcatg gcggagaaag
     8521 ggcttttgtc gtaaccggac agtacgaaaa ggttcttccc atggatatct atcccatgca
     8581 gctgctcaaa gccattcttg ccgaggatat tgagatgatg gagaatcttg ggatttacga
     8641 ggttgcggaa gaggatcttg ccctttgcga atatttatgc ccctctaaga ttgagataca
     8701 gtcgatagta agaaaaggta ttgaccttat gattaaggaa atgagctaat aaccagctac
     8761 cgcgatagat atgaattcat tgagaaagac actagataag cttaaacctc atttttcaaa
     8821 aggaggcaga ttttcaaaac ttcagtcggt atttgatggg atggagagct tcatgttcgt
     8881 tcccgacaag gtaacattca gtggatcaca tattcgtgat tatacggacc tgaagcggac
     8941 gatgaccatt gtaataatag ccctgctgcc tgcattgctg tttggttcat ataatacggg
     9001 attgcagcat ttcaggtcgg ttgggatcga atcgggaatt tttcagtcct tcggttatgg
     9061 tttcctgagg atcctgccgt ttataattgt aagctacctt gtcggcctgg gcattgaatt
     9121 cattgccgca cagatcaggg gacatgagat aaacgaggga tatcttgtaa caggcctgct
     9181 tattccgctc atcatgccaa ttgatgtgcc gctatggatg cttgcactgg cagtggcgtt
     9241 tgcagtaatc ctgggcaagg aagtgtttgg aggaacggga atgaatactt tcaatcctgc
     9301 cctgctggcc agggcgtttg tgtttttcag ctatcctggc tggatgacgg gtgacacggt
     9361 atggacagcg gggctcactg ccgggaaaaa tattgctgat ggattttccg gagccacacc
     9421 attgtcgctt tgcgtcacag gtgaaatatc caggttacct tctttcatgg aaatgtttgt
     9481 tgggacgatc ccggggtcgt ttggagagac atcagtcata gctattctca ttggtgccgc
     9541 ggtgctgatt gcaacaggtg tgggaagctg gaaaattatg gcaggtgttt tcggcggcgg
     9601 attggcaatg ggactcctgc tcaatgccat ttcagcaaat gaatacatgc atctgccgtt
     9661 ctactatcaa tttgtaatgg gcggatttgc cttcggtgca gtgtttatgg ccacagatcc
     9721 ggtaacagct gcgcaaaccg agaaaggcaa gtggatatat ggttttctga taggtgtgct
     9781 ctcgatcctt atcagggtgc ttaatccggc ctatcctgaa ggggtgatgc tggccattct
     9841 tctgatgaac gtattcgccc cgctgattga ttattttgtg attcagtcga gtattaacag
     9901 acgactaaaa agggtcaaag ttgaaattaa aacctctgta aaatgaagca attcagtaac
     9961 aggtatattt ttattttttc atcggtgatg gttattgcgg tggcagctct cctgtcgctg
    10021 gctgcaaccc tgcttcagcc cgcccagacc aggaacctcg agattgaaaa gaaacgcaat
    10081 atgcttgaat cgataaatgt tgctgctacc cgtgaaaata cagcggacct ttacggaaaa
    10141 tacataaagg aaggctttgt tattaattcc aggggagaaa tgattgaagg cgttgacgca
    10201 ttttcagtag tactcagaaa tgaacagaaa aagccgcttg cagagcagta tttgccggtt
    10261 ttcagggctg tacctgatga cggggagcag gttgtaatac tgccggtcga aggaaaaggt
    10321 ctttgggggc ccattttcgg atatatctct ttaagatctg atatgaatac catttacggg
    10381 gtcaatttcg atcataaggg ggagactccg ggccttggtg cagaaataaa tacctctgct
    10441 tttgaaagcc agttccgagg aaagaaactt tatgacaaca cggattttat ttccgtccat
    10501 gtcgtcaagg gaggcgccag ggaaggagat gtgcatggcg tggatgctat atcgggcgga
    10561 acaataacaa gcaagggcct cgaaatgatg attcacgatt gtatgctaaa atacaatgac
    10621 tttcttttaa aaaacaggaa ctgatatatg gacattatgg aaaaggaatc gatattttca
    10681 ccacggaatc tcaaactcct taccgaaccg ctggggaaga acaatccgat cacggtacag
    10741 gtacttggaa tctgttcagc actggctgtg acagtgaagc ttaagcctgc ccttgtgatg
    10801 tcgttatcgg tcatcatagt aaccggtatg tcgaatcttg tgatagctgc tttccggaag
    10861 tatatacctg aaaccatcag gatcattgtc cagctggtgg ttattgcaac actggttatt
    10921 cttgtcgacc agtttttaaa ggcatatgtc tttgatgtga gcaagcagct ctcggttttt
    10981 gtggggctga tcataacaaa ctgcataatt atgggcaggc ttgaggcctt tgccatggcc
    11041 aataagccct ggccctcatt ccttgacggc atagggaatg gtgccggtta cggtttgata
    11101 ctggtggtgg tggctttttt ccgcgaactg ctcggatcag gcacagttta tggttatccc
    11161 gtactggaaa agctcggttt gttcaaaacg ggttacgaaa acaacggcat ggtcattctg
    11221 cccccgatgg ctttgatttt tgtcggcctg atcatctggt ttcagcggag cagggacaaa
    11281 agcctggttg aaaaaaacta attgctaagc tatggaaaac ctgctgaata tcttcataaa
    11341 gtcgattttt gtcgacaata tgatctttgc ctttttcctt gggatgtgct cttatctggc
    11401 tgtttcaaag aatgtaaaaa ccgcgatagg gctgggaatg gccgtggtct ttgttatgtt
    11461 catcacggtt cccgttgatt atctcattga aaaatatgtt ctgaaaaaag gagcactttc
    11521 ctggctcgga gaaggatttg cagatgtcga tctcagtttc ctgacattca tggtttttat
    11581 tgcagtcatt gcagccattg ttcaactggt tgaaatggta gttgagaaat tcagccctgc
    11641 cctgtacaat tcactcggta ttttccttcc gctgattgcg gtgaactgtg cgattcttgg
    11701 cggttcactg ttcatgcagg aaagggagta tgccaacg
//
LOCUS       DAOOYG010000080        11657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_5826_length_11657_cov_8.161610, whole genome shotgun sequence.
ACCESSION   DAOOYG010000080 DAOOYG010000000
VERSION     DAOOYG010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11657
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5826_length_11657_cov_8.161610"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..2132
                     /locus_tag="PK288_07830"
     CDS             261..2132
                     /locus_tag="PK288_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18872.1"
                     /translation="MKPLLFKSAISLILLLSACSQKQESFTVKFGEVPTEKKWAISEL
                     NRDLPSDWSGARYLTFDMNSSTPQRFDLKLYDAEGVRILSIHPFQGAWVRASIPLVHF
                     QKMNTEGMDMAEIWKTPRPGYWIGFTDAVGPITEIDSLGVSMLMPAGSPILEMKNFRL
                     TMVPQDSILGPVPVVDEFGQWIPGDWPGKAGSMEDLKSSWNQEENELNSGNPEISRYG
                     GFTGKRYKSTGFFRVEKADGVWWFIDPDGYPFFSAGSNCVTPSAESARINGREYIYAE
                     MPPPEFRSRGRQSRGDQVSFYTWNLYRRFGDGWYEKWTDMTVKRMLSWGLNTVANWSD
                     PNLGKTQRIPYVATLRGWGIETGKMGMPDIYEPGYREKVDSAARYQCEPLRNDPFLIG
                     YFIGNEIPWPEREQELVDEILSGKETHMQKELRNYLAGGDTPERRKAFVYETYSKFLE
                     IVNSAVKKHDPNHLNMGIRYGGSPPDEIIKASKGFDVFSLNIYAYEPDQKVLQNIYNI
                     TGLPIVIGEFHFGTPGRGMAPGLAQTSNYEERGVAYRYYVENAASHPALIGTHWFQWI
                     DQPSTGRFDGENYNIGFLDVTDRPYPEMVSASREIFSRLYDVHSGKVPPVSRKALVN"
     gene            complement(2129..3493)
                     /locus_tag="PK288_07835"
     CDS             complement(2129..3493)
                     /locus_tag="PK288_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HPM18873.1"
                     /translation="MSKEDPPFSAERRNFLKTMAMGSAAGILGFAGSRADAAPYEAPA
                     YAKAMPPVKIRSVKAIPTAPSYSNLIVVKVETTEPGLYGLGCATFTQRAFPVVTAIEK
                     YMNEFCVGRDADNIEDMWQAFYVSSYWRNGPVLNNALSGLDEALWDIKGKRANMPVYQ
                     LLGGKCRFAVPVYAHASGRSIEEVVDNVRKYMDEGFKYIRIQLGGYGGVGGAAAIPDF
                     RKAGFGAESDHYMDEYAYLSNVPKLFEAVRKACGDQIELLHDIHERNQPIDVINMCRQ
                     LEQYRPFFIEDPLSPENTHWWKQLRESTTVPFAMGELFNNINEFVEPMVNHYFDFIRI
                     HISQIGGITPAMKVARLGEWFNVRTAWHGPGDVSPVGHAANAHIDLAVWNFGIQEGGY
                     FFTDKEKEVFPGCPECRDGYLYIKEAPGLGIDINEKEAAKYPINNKAPGWTVRKSDGT
                     IIRP"
     gene            3702..6116
                     /locus_tag="PK288_07840"
     CDS             3702..6116
                     /locus_tag="PK288_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18874.1"
                     /translation="MKRRKFVREAAIGSLAVTGLTGTIIQASSCARKTETASPAGEFK
                     SLSGFIPLNLEETGSEIRITGTTFSYTVCKITGLIISASVLGDEWLSGPLPDLWASPA
                     NDPRGEQYYARYSPDTRVRVTESLPYKITVVSQGLFSRINKTTIPLTYNLLYTFETDG
                     TVRVDLKVTADQDIVIRWLTLAEAGISAACRFAAHEHDQGEGPSTCRPVNYDVIAERE
                     INAEGKFIPWFHFGNDRGGLELVFPYSGRRHGGYTDTSPFPTGDPLGRPWEMFILKKT
                     GSALQCTVYGIRNCREVIGPGYSYEDTFFISALPGRTMRADANSVQVQWIGPHQYRSN
                     WRHPGEEDIRKWAAGGVNTIVGGANWFSGDYGNCSMPEETRQFLEICHRYGLRVIPYV
                     TFTDQEYQTPGFNETGPEWRIEPVTEFNYRSQLMCYGAEGWQEHWRREVSRIFDQFPF
                     DGLYIDFWAGKISCLNARHGCTGPYGRFTIEGLRRMARIAREMVDTKTRNGFILSNTN
                     ILPLAMINNWMDARLYGEWHNLEDTDPLALRIYYNSHRYGTGNVLLVSNVPKITGRTL
                     ALSGLFQGNPVLNHARTPGERMILESNALLLNAFGANKSAALNKFETDEITGSYKETG
                     LAGVSLFHNRENGDLLICLSSFSKTGTRVKLRPLLESIIAGIPGFQEPVDATSTQMPY
                     YLFVFEDRNLIGGRPLLLADMGAFTLDLPADDYRNLWVRPATGARRILYSLNNAEPPD
                     EDYRPDDASMTVSIPSLARLSAETAFLGSSPESVGSPEGPVRFIPHNGWWEAELPCNT
                     DITVKY"
     gene            complement(6150..7115)
                     /locus_tag="PK288_07845"
     CDS             complement(6150..7115)
                     /locus_tag="PK288_07845"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18875.1"
                     /translation="MKKNLLFVIRGRLAASLRRLPMSIIVVSALLAALMRPDQAGAQI
                     HTFTKEQMEAFTALNPYGRFPDGRPMIPDAVIDSVRNLEIKVIEALEALAARKYNNSQ
                     FDAGWKTLIPGKKIAGRAFTVQFMPPRPDLQEAMQNEAKKNNTTAISKSATIDMLQKN
                     DLAVIDLYGKVEGGLPYVGDKLAYYIWKTTGTGFIVDGGIYFLETMAQSGIQAFYRGG
                     TPLTNTGFAIVTGVNIPIRIGDAVIMPGDLVIGDEDGILAIPPHLVTDVIKTTIANRR
                     RDIWIKKAFDLGRYKSSEIYGRMQEPLQKMFNDYRATGDPKYLPK"
     gene            complement(7413..8456)
                     /locus_tag="PK288_07850"
     CDS             complement(7413..8456)
                     /locus_tag="PK288_07850"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPM18876.1"
                     /translation="MVRKETFGTHGGKEVFRLTLTNKAGNVIRLTNYGARIVWIEVPD
                     KNGKRDNVTFGYDTFEGMINGDMSFGSVVGRYANRIAGGRFSIDGVEYKLPLNNGPNT
                     LHGGPGGWHSVVWDTEVLKNTENPSVRFTYHSPDMEMGFPGNVKAEVVYTWTDDNEIV
                     MDYSCTTDKKTVINVTNHAYFNLHGAGNGDILDHQLTLRASAFLPIDSVSIPTGEIRP
                     VAGTPFDFTEPHAIGERINQDYEQLKMGRGYDHNFILDNREPVDVTVYDPVSGRILEV
                     ITDQPGMQLYTGNFLDGSQVGYGGKPYNFRTGFCLESGHYPDSPNRPEFPSTLLNPGE
                     VFKSRTIYRFSTE"
     gene            complement(8689..9834)
                     /locus_tag="PK288_07855"
     CDS             complement(8689..9834)
                     /locus_tag="PK288_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18877.1"
                     /translation="MQKEYFIFIPGVAGTFEEEWGQCFAGAAEKTGEGYRLIKLNVFI
                     DPENSEIYLRQKKFIENFLKNRLGDQCPAISVITQPPEDPWKISAEGMFIRAGTTEVT
                     TKFWGSVPYVVLGPEPGKELWAAGLGDDELSENTRSASVKSFEMMSGILDQEGMSYNN
                     LVRQWNYVGEILKISRRYQNYQTFNEVRSEYYSKYRTAPGFPAATGIGMKIGGVIIDF
                     CAIQDSPAVRTRPIDNPKQVNAYQYSQKVLKGLKVKGKPIKNPPQFERALFVANGDKL
                     TLFVSGTASIIGQDTIGKENIKEQTHITIENIKRLSEITYLGELTGYDLSHGKCTLIR
                     AYVKRQEYFSAVKDICREQFPGVPAIYTEADVCRDDLLVEIEAEYAF"
     gene            complement(10101..>11657)
                     /locus_tag="PK288_07860"
     CDS             complement(10101..>11657)
                     /locus_tag="PK288_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPM18878.1"
                     /translation="DGEKTEIKSLQAEPWGQFLRYEYLKFDFSDISGEGMYFVKYGKQ
                     QSQPFRIAADVYKRNVWQPTIEFFLPVQMCHMKVYEKYRVWHGLCHMDDALMAPVNHN
                     HFDGYIQGESTLTRFSPGDHVPGINTGGWHDAGDYDLRVESQSSEVNILSMIYETFGI
                     DYDETTIDQAARIVEIHQSDGKPDILQQIEHGLLSIAGGYKSLGRFYRGIIESDKRQY
                     VLLGDGSTMTDGLIFDKNLKPGERTGTHSSLTDDRWVFTEDNPPRELTVAAHLAAGAR
                     ALKGYNDGLARECIEIAETVFNNEYKISGMTANAKVHAAIELFLTTRKDKYRNFILEN
                     GETVVKGISQLGWIIGRAIPEINNAGFTSLVRKAAADLSEQINRQSEETPFGVPYRPH
                     IWGAGWDIQSFGVRQYFLYTAFPDIFRPEPVLNALNFILGVHPGENTSSYASGVGARS
                     TTIAYGINRADESYIPGGVSSGTALIRPDFPELLDWPYLWQQQEYVMGGGATNFVFLV
                     LAADDLLDKK"
BASE COUNT         2930 a         2874 c         2726 g         3127 t
ORIGIN      
        1 attcaaagag cgtctaaagt gcgatctact aaaggtttgc tcgtgcatgt ctttggaaaa
       61 atcgcagcag ctctcatcta cttaattttt aactcttaat tcgcattaat gataatttct
      121 ttttgagtct gaaaaatcaa atctcgctga aaggatccat catctgaaaa agacccctta
      181 taagaatcct tattttatac tttggagaag gtgcgaaatg ttttttaatt ttcttaaatt
      241 cgatacttta tacaaatcca atgaaaccat tattatttaa atccgccatc tctctgatcc
      301 tgcttttgtc agcctgctct caaaagcagg aatcattcac cgtgaaattc ggggaagtcc
      361 cgactgagaa gaaatgggcc atcagtgagc ttaaccgtga tctgccctcc gactggtccg
      421 gagcccgtta tcttactttc gatatgaact cgtcgacccc gcagcgattt gatctgaagc
      481 tctacgatgc tgaaggcgta aggatcctat cgattcatcc gtttcaggga gcctgggtgc
      541 gggcatcaat tccactggtt catttccaga agatgaatac cgaaggaatg gacatggctg
      601 aaatctggaa gacgccaaga cccggttact ggatcggatt caccgatgct gtgggaccga
      661 taactgaaat tgactccctt ggagtttcaa tgctgatgcc ggcgggctct ccgatactgg
      721 agatgaagaa tttccgtctt accatggttc ctcaggactc catactggga ccagtaccgg
      781 tggtcgatga attcgggcaa tggataccag gtgactggcc aggtaaggcc ggttcgatgg
      841 aggatctgaa atcttcatgg aaccaggaag aaaatgagct gaattccggc aatcctgaga
      901 tttcaaggta tggtggattt accgggaaga gatataagtc cacaggattc ttcagggttg
      961 aaaaagcaga cggagtctgg tggttcatcg atcctgacgg gtatcctttt tttagcgccg
     1021 gcagcaactg tgtaactccc agcgctgaat cagcccgcat caatgggcgg gaatacattt
     1081 atgccgagat gccgccgcct gaattcagaa gccggggccg tcagagcaga ggcgaccagg
     1141 tttcattcta tacctggaac ctgtaccgcc ggtttggcga tggctggtac gaaaaatgga
     1201 ccgacatgac tgtaaagagg atgttaagct gggggctcaa tacggtagcc aactggtcgg
     1261 atccgaacct gggcaagact caacggatcc catatgtcgc cacactgaga ggatggggca
     1321 ttgagaccgg gaagatgggg atgccggata tttacgagcc cggttacagg gagaaggtcg
     1381 attcagctgc aaggtatcag tgcgaaccat tgaggaacga tccgtttctt atcggttatt
     1441 ttataggcaa tgagattccg tggcccgaaa gggagcagga gctggtcgat gaaatacttt
     1501 ccggaaagga aacacacatg cagaaagaac tcaggaatta tctggcagga ggcgacacac
     1561 cggagcgccg gaaggctttc gtatacgaaa cctactcaaa attccttgag attgtgaaca
     1621 gtgccgttaa aaagcatgat cccaatcacc tgaacatggg gatcaggtac ggaggctctc
     1681 ctccggatga aattataaag gcttcaaagg gctttgacgt gttcagcctt aacatatacg
     1741 cttatgaacc tgatcagaaa gtcctgcaga atatttacaa tattacaggg cttcccatcg
     1801 tgatagggga attccatttc ggaacgcccg gacgcgggat ggctcccggc ctggcgcaga
     1861 caagcaatta tgaagagcgg ggcgtggcct atcgctatta tgttgagaat gcggcttcac
     1921 atccagccct gatagggaca cactggttcc agtggatcga ccagccctcg acaggccgtt
     1981 ttgacgggga gaattacaat ataggcttcc ttgatgtcac cgaccgtccg tacccggaga
     2041 tggtcagtgc atcaagggaa atcttcagta ggctttatga tgttcattcc ggcaaagtgc
     2101 cgccggtaag ccggaaagct cttgtcaatt agggtctgat tattgtcccg tcagatttgc
     2161 ggacggtcca gcccggagcc ttgttattga taggatactt tgcagcttcc ttctcattga
     2221 tgtcaattcc aagtccgggt gcctccttta tgtaaagata accatccctg cattcggggc
     2281 atcccggaaa gacttccttt tccttgtcgg tgaaaaagta tcctccttcc tggatcccga
     2341 aattccatac tgcaagatca atatgagcgt ttgcggcatg gcctaccgga gatacatccc
     2401 cggggccgtg ccatgctgtc cgcacattga accattcgcc gagcctggca accttcatgg
     2461 caggggtgat cccgccaatc tgtgatatat gtatccttat gaaatcaaag taatgattta
     2521 ccatgggttc cacgaactca ttgatattat tgaaaagttc tcccatagca aaagggacag
     2581 tggtgctttc acggagctgc ttccaccagt gggtgttctc gggtgagagc ggatcttcaa
     2641 tgaaaaaagg cctgtactgc tcgagctgcc tgcacatatt gatcacatca atcggctggt
     2701 tccgctcatg aatgtcatga agaagctcaa tctgatcacc gcaggcctta cgcactgcct
     2761 caaacagttt cggcacattc gacagatagg catattcgtc catgtaatgg tcggactccg
     2821 ctccaaaacc cgcctttctg aaatcaggta ttgctgcggc tcccccaact ccaccgtatc
     2881 ccccaagctg gatcctgatg tacttgaatc cctcgtccat atattttctg acattgtcaa
     2941 caacctcttc gattgatctg ccgcttgcat gggcataaac cggcacggca aacctgcatt
     3001 tgccgccgag cagctgatag accggcatgt tcgccctttt gccctttatg tcccacaggg
     3061 cttcgtcgag tccgctgagt gcgttgttaa gtaccgggcc gtttctccag taggagctta
     3121 catagaaagc ctgccacata tcttcgatat tgtcggcatc cctgcccaca cagaattcat
     3181 tcatgtactt ttcaatcgca gttacaaccg ggaaagcacg ctgggtgaat gtggcacaac
     3241 ccaggccata caaccccggt tccgtggttt ccaccttaac cactatcagg tttgaatagg
     3301 atggcgcggt aggaattgcc ttaacgctcc tgatctttac cgggggcatc gcttttgcat
     3361 atgccggggc ttcgtaaggg gcagcatctg cccttgatcc ggcaaaacca agaattccgg
     3421 cagcagagcc cattgccatg gtcttcagga aattccttct ttcagccgaa aacggaggat
     3481 cttctttgct catgatagtt taatttagat tagtacgatc tcattctaca cagatttaaa
     3541 aaggccagtc agctgagtca ggtattactg actggttttt gcatgaacta aaccattctg
     3601 ttatttttct taaatttatt gaaatgaact ttaagatcaa acagtccggc aaaaaaattt
     3661 aaacttgcca tttaacatca aaatgtaatc ggatatcaac catgaagcgc agaaaatttg
     3721 tccgtgaggc agcaatcggc agcctggcag ttacaggttt aacaggaacc atcattcagg
     3781 catcgtcatg tgcccggaaa acggaaacgg catcccctgc cggcgagttt aaaagccttt
     3841 caggattcat tcccctgaac cttgaggaaa ccggatcgga gatcaggata accggtacca
     3901 ctttttccta caccgtctgt aaaataaccg ggctcatcat cagtgccagt gtgctgggcg
     3961 atgaatggct ttcaggtcct cttcccgatc tgtgggcctc tccggcaaac gacccccggg
     4021 gtgaacagta ctatgcccga tactcacccg acacccgggt cagggttacc gaatcccttc
     4081 catataagat tacagttgtc tcacaaggat tattctcccg gataaataaa actaccattc
     4141 cgcttactta taatctgtta tacacttttg aaacggacgg gaccgtaagg gttgacctga
     4201 aagttacggc agatcaggac attgtcatcc gctggcttac ccttgctgaa gcgggcattt
     4261 cagcggcatg ccgttttgcg gcacatgaac acgaccaggg tgaggggccg tctacctgca
     4321 gaccggttaa ttacgacgtt atagctgaaa gggaaattaa tgccgaaggg aaatttatcc
     4381 cgtggttcca ttttggaaat gacaggggcg ggctggagct tgtatttccc tattccggca
     4441 gaagacatgg cggctatacc gataccagcc cgttcccgac aggtgatccg ctgggccgtc
     4501 catgggaaat gttcatcctg aagaagacag gatctgcact tcagtgcact gtttatggta
     4561 tccgcaactg cagggaggta atagggcccg ggtacagcta cgaggatacc tttttcatct
     4621 cagccttgcc gggcaggacc atgcgcgctg atgccaactc ggtccaggtg caatggatcg
     4681 gcccgcacca gtacagaagc aactggagac atccgggaga agaggatatc aggaaatggg
     4741 cagccggcgg ggtcaatacc attgtcggtg gtgccaactg gttctcaggc gattatggaa
     4801 attgcagcat gccggaagaa acaaggcagt tcctggaaat atgccacagg tacggtttac
     4861 gggtgatacc gtatgtaaca ttcactgacc aggaatatca gactccagga ttcaatgaaa
     4921 ccggtcctga atggagaatc gaacccgtta ccgaattcaa ttacaggagt cagctcatgt
     4981 gctacggagc tgaaggctgg caggaacact ggcgcaggga agtgagccgg atattcgatc
     5041 agtttccgtt tgacggactc tacatcgatt tctgggccgg aaagatcagc tgcctcaatg
     5101 caaggcacgg ctgtacggga ccgtacgggc gatttacaat agagggcctg cgcagaatgg
     5161 ccaggattgc aagggagatg gttgatacca aaacaaggaa cggattcata ctttcaaata
     5221 caaatatcct tcccctggca atgatcaata actggatgga tgccaggttg tatggtgaat
     5281 ggcataatct cgaagacact gatccgctgg cgctgcgcat ctattataac tcacacaggt
     5341 acggaaccgg gaacgtactg ctggtaagca atgtaccaaa aataaccgga cgtacgcttg
     5401 cactgtcggg gctgttccag ggaaatccgg tgctgaatca tgcccggact cctggcgaac
     5461 gcatgatcct cgagagcaat gccctgctcc tgaatgcttt cggggcaaat aagtctgccg
     5521 cactgaataa atttgaaaca gatgagataa ctggttccta taaggaaacg ggacttgccg
     5581 gggtatcact gtttcataac agggaaaacg gggatctgct catttgcctc tcttcctttt
     5641 ccaaaaccgg tacaagggtt aagttacgac cgctgcttga atcgattatt gcaggaatcc
     5701 ccggatttca ggaaccagtt gatgctactt caacacaaat gccatattac ctgtttgttt
     5761 ttgaagacag gaacctgatc ggtggacggc ctcttttatt agccgatatg ggtgcattca
     5821 ccctcgatct gcccgccgat gattacagga acctgtgggt aaggcctgca accggtgcgc
     5881 gccggattct ctacagcctg aacaatgcag aaccgccgga tgaagactac aggccagacg
     5941 acgcttcaat gaccgtttca attccttccc tcgcccgcct gtcagcagaa acagctttcc
     6001 ttggcagcag tccggaatcg gtgggctcac cggaagggcc tgtaaggttc atccctcaca
     6061 atggctggtg ggaagctgaa ttgccgtgca atacggacat taccgtgaaa tactgatccc
     6121 ttagattact gccaggaaat cctgagagat cattttggca gatatttggg atctcccgtg
     6181 gccctgtaat cgttgaacat cttctgaagc ggctcctgca tacggccgta aatctcactc
     6241 gacttgtacc tgccgagatc aaaggctttt ttgatccaga tatcacggcg gcgattggca
     6301 atggtggttt ttataacatc cgtaacaaga tggggaggga tggccagtat accatcctca
     6361 tcgcctatca caaggtcgcc aggcataatt acagcatccc caatgcggat cgggatattg
     6421 acgccggtaa caatggcgaa tccggtattt gtaagcgggg taccgccgcg gtagaaagcc
     6481 tgtatcccgc tctgggccat ggtttcaagg aaataaatcc ccccgtcgac aatgaacccg
     6541 gtgcctgtgg ttttccatat atagtaagcc agcttatctc ccacgtacgg caggcctccc
     6601 tcaaccttcc cgtaaagatc aatgacagca aggtcatttt tctgaagcat atcgatggtt
     6661 gcggatttgc ttattgccgt tgtattgttc tttttggctt cattctgcat tgcttcctgc
     6721 aggtcgggcc ttggaggcat gaattgtact gtaaatgccc tgccggcaat ttttttccct
     6781 ggtataagcg tcttccaccc ggcatcgaac tgactgttat tatacttcct tgctgccaat
     6841 gcctccagtg cttctataac cttgatttcc aggttcctca cggagtcgat cactgcatcg
     6901 ggaatcatcg ggcggccgtc aggaaaacgg ccatagggat tcagagccgt aaaagcttcc
     6961 atctgttcct ttgtgaaggt atggatctgt gcaccggcct ggtcaggcct cattaatgcc
     7021 gcaagtaatg cggatacaac gataatgctc atcggcaggc gcctgagact ggcagctagt
     7081 ctgccccgga taacaaacaa taaatttttt ttcatcagta tttagtttac tttttaaaag
     7141 atcctgaacc tttctgatcc ctggcacagg gaaccacaaa tgatgaacgc ggttattttc
     7201 atttgtgtcc atggtcagtc atgcagattc catcacaatt aaatcattat tgaaagcctg
     7261 gtgtccgacc gacatgcagg gcaggcattt caaataccag aatattatgc acatgcctca
     7321 ttgtccgaca tatcgggaat tcacgggctg atggttctga cctgatattc tttgcatgca
     7381 ttccggccaa aggaggagaa acattataac attcactcag tcgaaaacct gtaaatcgtc
     7441 cttgatttga atacctcacc cggattaagc aatgtcgaag ggaactccgg gcgatttggg
     7501 ctgtcagggt aatggcccga ttccaggcag aagcctgtcc tgaaattata gggtttcccg
     7561 ccatagccaa cctgggaacc gtcgaggaaa tttccggtgt aaagctgcat acccggctga
     7621 tcggttatga cctcaagaat ccggccgctt accggatcat aaacagtaac atcgacgggc
     7681 tccctgttgt cgaggatgaa gttatgatca tatccgcgcc ccattttcag ctgctcataa
     7741 tcctggttta tcctttcccc gattgcgtgg ggttccgtaa agtcaaacgg agtacctgcc
     7801 accggtctga tctcaccggt cggtatcgac actgaatcaa tcggcagaaa agctgatgcc
     7861 ctgagcgtca gctgatgatc gagaatgtcg ccgttgcctg ctccgtgaag attgaaatat
     7921 gcatggtttg tgacatttat cacagtcttt ttatcagtgg tgcaactgta atccattaca
     7981 atttcgttgt cgtcggtcca ggtataaact acctcagctt tcacgtttcc gggaaatccc
     8041 atttccatat cggggctatg atatgtgaac ctgacggaag gattctcagt atttttcagc
     8101 acctctgtgt cccataccac gctgtgccag ccacccggcc ctccgtgaag ggtgtttgga
     8161 ccgttgttca gcgggagttt gtattcaacc ccgtcaatgg agaatctgcc gccggctatt
     8221 ctgtttgcat acctgcccac gacagaaccg aacgacatgt cgccgttaat cattccctcg
     8281 aaggtatcgt acccgaaggt tacattatcc cttttcccgt ttttatcagg aacttcgatc
     8341 catacaatcc tggcaccgta gtttgttaat cttataacat tgcctgcctt gttggtgagt
     8401 gtcaggcgaa atacctcttt tccgccgtga gtgccgaaag tttcttttct gaccatttct
     8461 ttggtttttg cagaatgttt gcatgaaatc gcaagtccca atactaagga cagcgccagt
     8521 gaaaataata aaatcttttt catttttcag agtatgtttg ggaatatata gatcctgtcc
     8581 tgacagtgat ccttttcctg taaatttgac actgttacgt tccggaatgc gcttcagatc
     8641 cggataatgc acaggtactg agccagtgac cgtatttcgg ggaatatttc agaaagcata
     8701 ttccgcctca atctcaacca gcaggtcatc cctgcagaca tcagcttcag tgtatattgc
     8761 cggtacgccg ggaaattgct ccctgcatat gtcttttacg gcactgaaat attcctgccg
     8821 ttttacatat gcccttatca acgtacactt cccatgcgac agatcgtaac cggtgagttc
     8881 accaagataa gtgatttcgc tgagtctttt gatattttca atggtaatat gtgtctgttc
     8941 ctttatgttc tcttttccaa tggtgtcctg tcctattatt gatgccgtac ctgacacgaa
     9001 cagggtcagt ttgtcaccat ttgcaacaaa aagtgccctt tcgaactgcg gagggttctt
     9061 tatcggttta ccctttacct tcaatccttt cagcactttc tgactgtact ggtaggcgtt
     9121 gacctgtttc gggttatcaa taggccgggt cctcacagcc ggggaatcct ggattgcaca
     9181 gaaatcaatg ataacgcctc cgatcttcat ccctatacct gtggctgccg gaaaacctgg
     9241 cgctgtcctg tacttggaat agtattcgct tcttacttca ttgaaagtct gataattctg
     9301 ataccttctg ctaattttca ggatctcccc aacgtaattc cattgcctta ccagattgtt
     9361 atagctcatt ccttcctggt caagtatccc tgacatcatt tcaaatgact taacggaagc
     9421 ggacctggtg ttttcagata attcatcatc gcccagccct gcggcccaga gctcttttcc
     9481 cggttccggc cccagcacca cgtatgggac agatccccag aattttgttg taacttctgt
     9541 ggttccggcc ctgatgaaca ttccttctgc agagatcttc cacggatctt ccggaggttg
     9601 ggttataaca ctgattgccg ggcattgatc ccccagcctg ttcttcagga agttttcaat
     9661 gaattttttc tgccggagat atatttcgga attctccgga tcaataaaaa cattcagttt
     9721 tattaacctg taaccttctc cggtcttttc cgccgccccg gcaaagcatt ggccccattc
     9781 ttcttcaaag gtgcctgcga cgcccggaat gaaaataaaa tactccttct gcatgagaat
     9841 aagtcattat tctcaaataa agcacatcga attgaaatta acaaatgtta acatattgtt
     9901 aaatttggta attttcttat aatgtgaaat atacagacag aagtgtctga aaaaattgtg
     9961 taataaatta tttctttcac aaagttcaac tgataagctc aaagtatccc agagaatggt
    10021 tatattaact ctgtgcctcc ttactgtatc tctgtgaaaa ttcttaaaaa atgtttcgga
    10081 gaaacacaaa tcatgaaatt ctatttctta tcaaggagat catcagccgc aagaacaagg
    10141 aaaacaaagt ttgttgcgcc gcctcccata acatattcct gttgctgcca cagatagggc
    10201 cagtcgagca gttcggggaa atcaggcctg ataagagccg tgcccgatga tactcccccc
    10261 ggaatatatg attcatcagc cctgtttata ccataggcaa tggttgttga ccttgctccc
    10321 acccctgatg catatgatga agtattttca cccgggtgaa ccccaaggat gaaattaagt
    10381 gcattcagga cgggttccgg cctgaaaata tcagggaacg cagtataaag gaaatactgc
    10441 ctcaccccga agctctggat atcccatccg gctccccata tatgtggcct gtaaggtact
    10501 ccgaaaggag tttcctccga ttgccggttt atctgttccg aaagatccgc cgcagctttc
    10561 cttaccaggc tggtgaaacc agcattgtta atttccggaa tcgcccttcc tattatccag
    10621 ccaagctgac ttatgccttt taccactgtt tccccgtttt cgaggatgaa gttcctgtat
    10681 ttatccttgc gggtggtaag aaacagttcg attgcagcat gtacctttgc attggcagtc
    10741 atacctgaaa tcttgtattc attattgaaa acagtctcag ctatttcaat gcattcccgt
    10801 gcaagaccgt cattgtatcc tttgagtgct cttgctccgg cagcgagatg agcagcaacg
    10861 gtaagttccc tcggagggtt gtcttcggta aatacccacc tgtcgtctgt aagtgaggaa
    10921 tgggttccgg tccgctcacc cggtttaagg tttttatcga aaataaggcc gtcagtcatg
    10981 gtggaaccgt cgccgagaag aacatactgc cttttgtctg actctataat tcccctgtaa
    11041 aaccgtccaa ggcttttata ccctccggca atcgatagca atccatgctc aatctgctgg
    11101 agaatatcgg gttttccgtc ggactggtgg atttcaacga tcctggctgc ctggtctatg
    11161 gtagtttcat cgtaatcaat tccaaaagtc tcgtagatca ttgaaagtat atttacctca
    11221 gaactttgcg attccacacg gaggtcgtag tctcctgcat catgccatcc tcccgtattt
    11281 atgcccggca catgatcgcc cgggctgaat cttgtaaggg tagattcccc ctggatgtat
    11341 ccgtcaaaat gattgtggtt caccggtgcc atcagtgcat catccatatg gcacagcccg
    11401 tgccatacac ggtatttttc atacactttc atatggcaca tctggacagg gaggaagaac
    11461 tcgattgtcg gctgccagac atttcgttta taaacatcgg ctgctatcct gaaaggttgt
    11521 gactgctgtt taccgtattt cacgaaatac atcccttccc cgctgatatc tgaaaaatca
    11581 aatttcaggt attcatagcg gaggaattgt ccccacggtt cagcctgtag tgattttatt
    11641 tctgtctttt ccccgtc
//
LOCUS       DAOOYG010000081        11286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6118_length_11286_cov_6.200695, whole genome shotgun sequence.
ACCESSION   DAOOYG010000081 DAOOYG010000000
VERSION     DAOOYG010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6118_length_11286_cov_6.200695"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PK288_07865"
     CDS             <1..553
                     /locus_tag="PK288_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPM18879.1"
                     /translation="RAAGFYNTLLEGDDPAIVIEPLNGYRIKEKMPDNIGELKLPLGI
                     PEVITEGDDITIVTYGSTVRIAAEATEQLSKHGISAELIDVQTLVPFDTERRIIESIR
                     KTNRILFLDEDVPGGTTAYMLQEVLEKQGGWNYLDSPPKTLTGKEHRPAYTTDGDYFS
                     KPNAEDIFDMVYAMMKESVPARY"
     gene            complement(620..2389)
                     /locus_tag="PK288_07870"
     CDS             complement(620..2389)
                     /locus_tag="PK288_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HPM18880.1"
                     /translation="MQKSTLRIVGNKVVLNIRERMCETADELLASEKFRKILDHSIKH
                     LEDIGSPLLDIFENGKVDEESVTSLVKTLTLLFKYEGSVVPHIFDKSSVFLRDTHLLS
                     GFVEYLYDYWRHFDRFIINDSEGDRLDKRPYRTFNETIEHLTHLIRTVYRHVQENITG
                     NHPTVYRQVPAGAEMAVISLPFEITWPDPKYQFLNSIPVTRQILIYPPLVIHQPMNKR
                     TGDFRLIQQNPIDLIKLDPEDWLCYPAKVGKLVIMIYFHITFLELGLSMCNLFQVAED
                     EDLAKKPDAVFIYGAPPETFDSISKYSTVFHHDKANNLFVGAIPGRLEFGYFGYLKKM
                     VLTLHNSIIMQSGNLPFHGAMVKILLKGRKEATILMIGDTGAGKSETLEAFRVLGEKE
                     IRELRIIADDMGSIELDNDGNPVGFGTEIGAFLRLDDLQPGYAYGHLDRSIIMNPSQV
                     NARIVIPATNFATIIKGHKIDYILYANNYEEIDADHPVIEKFDNVDWALDVFREGAVM
                     SKGTTTSTGLVHSYFANIFGPPEYRELHEKIAKRFFESFFSKNIFVGQMRTRLGILGH
                     ETSGPLEAAKELLTIIGSKAEGL"
     gene            2528..3118
                     /locus_tag="PK288_07875"
     CDS             2528..3118
                     /locus_tag="PK288_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HPM18881.1"
                     /translation="MKPELDLFAIHPEDKIPEKGKVLISEPFLADTFFNRSIVYLTDH
                     NQKGSVGFILNKKLDIKICDAVSGFEGWDENLNMGGPVAPDTLHYLHSLGNLIPKSVP
                     VDGTIYWGGDIETIKGLIRTNAIKPYQIKFFLGYSGWSAGQLDRELKENSWVIARVKS
                     EIVMSAWGSDSWKRLLRSFNNKYRIWADFPESPEMN"
     gene            complement(3115..4125)
                     /gene="holA"
                     /locus_tag="PK288_07880"
     CDS             complement(3115..4125)
                     /gene="holA"
                     /locus_tag="PK288_07880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPM18882.1"
                     /translation="MPLTYEDIISDLTKRIFRPVYFLAGDEPYYIDLITDFIGDKVLS
                     EQEKAFNQLILYGEDTTIESIIDTARRFPMMASHQVVIVKEAQSVKKIENLIFYLEKP
                     LLSTILVLNYKYKTIDKRTKFYKALETHGIYFESKRLYDNQVPPWIERYLMTRGIRIE
                     PFASAMLTEFLGTDLHKIANELDKLLITLPAGKQVITTELIEKNIGISREYNNFELQK
                     AVGEKNILRANRIINYFSENQKDNPVTLTIASLFSLFVRILTYHYIADKSRNNVAAML
                     KINPFFVKDYETAAARYNISETVRVIELLRTFDMKSKGWGDTGADSGDLLKELIYKIL
                     HI"
     gene            complement(4128..4898)
                     /locus_tag="PK288_07885"
     CDS             complement(4128..4898)
                     /locus_tag="PK288_07885"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPM18883.1"
                     /translation="MKTKADIINNWLPRYTGKSLDTFGKYFLLTNFVYYVELFAQINN
                     SEVEGRDKNWPSSTADGITIICFGMGSPNAATVMDLVSAVEPEAVLFLGKCGGLKKKN
                     TLGDLILPIAAIRSEGTSNDYLPLEVPALPAFQLQSAISASITRHNKEYWTGTVYTTN
                     RRVWEYDDRFKKYLVKTRAMAIDMETATIFTVGFANEIPTGALLLVSDQPMISTGIKT
                     SAIDKNVTDKFVEMHLKIGIDALREIKNNGISVKHLRF"
     gene            4960..5421
                     /locus_tag="PK288_07890"
     CDS             4960..5421
                     /locus_tag="PK288_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPM18884.1"
                     /translation="MIQLNLPSYSFRITGSEGSEMIFDPIRRKYVRLTPEEWVRQNFV
                     QYLIHEGKYPPGLMETEVMFRLHNKLVKRIDIRVRDRRGNPVLLVECKSYKVAINDAK
                     VRDQIGEYNMHFKVPYVVITNGLDHYAFRYDQERNLYDFMMVIPLYEDLLT"
     gene            complement(5423..6586)
                     /locus_tag="PK288_07895"
     CDS             complement(5423..6586)
                     /locus_tag="PK288_07895"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPM18885.1"
                     /translation="MDFKSLASLYRDELLDNVLPFWLNNSIDREMGGYFTCLLRDGKV
                     FDTDKFIWLQGRQVWMFSMLFNKLERKQEWLECAIHGGEFLKKHGHDGNFNWYFSLAR
                     DGRPLVEPYNIFSYTFAAMAFGQLSLATGKQEYADIARKTFDLILSRAANPKDRWNKA
                     YPGTRNLKNFALPMILCNLSLEIEQLIDREFLEKTIETCIHEVMDIFYRPELGLIVEN
                     VTEDGALSDTFDGRLFNPGHALEAMWFIMDLGKRMNRPELITKANEIALRTIEYGWDR
                     EFGGILYFMDRLGYPPQQLEWDQKLWWVHIETLITLLKGYLLTGTANNLEWFDILHDY
                     TWDHFKDREHGEWFGYLNRRGEVLLPLKGGKWKGCFHVPRGLYQCWQILEKLA"
     gene            complement(6604..7977)
                     /locus_tag="PK288_07900"
     CDS             complement(6604..7977)
                     /locus_tag="PK288_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HPM18886.1"
                     /translation="MGYIVFLALVAALGGFLFGYDTAVISGTITQVSSQFNLSPLQEG
                     WYVGSALVGSIMGVAVAGVMSDFLGRKKTMIISAILFMVTGIGCALSNTINGLVAYRL
                     VGGIGIGVASIVSPIYISEISVAKHRGRLVSFYQLAITIGFLGAYLANFQLLKLAAGG
                     NWANPVLNKIFVSEVWRAMLGMETVPAVMFFFMIFFIPESPRWLILKHSPEKASRILK
                     RIYATDDEVNNQIAQTQQVVQTEKKSEWKFLAEPGILKAVLIGVAIAMLGQFMGVNAV
                     LYYGPSIFTDSGLSSGDSLFYQVLVGLVNMLTTVLALIIIDKVGRKQLVYWGVSGMII
                     SLICIAIYFAFGKEWGLTPVILLVFFLFYIFSCAISICAVIFVLLSEMYPMRVRGLAM
                     SIAGFSLWIGTYLIGQLTPWLLENLKPSGTFLLFALMCFPYIWIIWKKVPETTGKSLE
                     EIEKMWT"
     gene            8136..9146
                     /locus_tag="PK288_07905"
     CDS             8136..9146
                     /locus_tag="PK288_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPM18887.1"
                     /translation="MNRVSCAVIRNEENEILILQGEKDAGDPLRWEFPYGSPAEDETD
                     EECIIRVTREQLGIDIVICSLMEIVEYDCGYGQISLTPFICDTLDELPFLTVHTAFRW
                     ITPDELRNIEFTGFNVQVAHDYFSDHIGQGNRCEETLAGEFSNLDSDDAIRLMVGSFK
                     GAREAELLAGSAIANGEVLKKLLDYSFSDDRKLAFRASWTLSKLHDKIPEIVTPYLGR
                     LIGALDTIDNESVQRSFLRIVSLSDLNLVPARDQGMLADHCFRMLKSGFSAIAVKAYS
                     IEIIYKLALIYPELSGELAATVRLLEDEAPTGVLAKGRNLLRKLAAKQGEADPSGMNI
                     YD"
     gene            9179..9793
                     /gene="yedF"
                     /locus_tag="PK288_07910"
     CDS             9179..9793
                     /gene="yedF"
                     /locus_tag="PK288_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HPM18888.1"
                     /translation="MKVIDTSGLQCPAPLIATRRAMKETEEGESFGIITDSKSSFENI
                     SRFLKDHGQEFSCEESSGKWILTINKGPTGNISVSPAGYCETPVPHFSKGDFIVAFGS
                     DKMGEGNDELGRHLMINFVLALKDLDKLPVRMVFYNSGVLLGSEDSKAADHLNEMEKM
                     GIELLFCETCVSYYSLSKKMKVGTLSNMFEIAQIMASASNIVKP"
     gene            9812..10852
                     /gene="selD"
                     /locus_tag="PK288_07915"
     CDS             9812..10852
                     /gene="selD"
                     /locus_tag="PK288_07915"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPM18889.1"
                     /translation="MKIDLLEMADGGGCSSKIPALQLEEFLKHLPLPEDPDILVNISN
                     HDDAGVYRINDELALVFTNDFFPPVCSDPYEFGQIAAVNALSDVYAMGGRPVVALNIM
                     MFPSSRLSPDVYAGILRGGYETAAGAGVSIIGGHTIDDPVPKYGLAVIGFVHPSGIIT
                     NAGAMPGDSIILTKPLGTGIILAANRLGLASQADISEALASMKQLNKPGADIMKKYGV
                     KGATDITGFGLAGHTLKMARASEVSITLNMKQVRLIGNTLALVEDGCIPGAAFRNLDY
                     IEKDTRFSAGLAYNLKMAACDAQTSGGLLISAAPEIAGQVVDELSETGFHKAAIIGKV
                     TERSEKLLYLNE"
     gene            complement(10886..>11286)
                     /locus_tag="PK288_07920"
     CDS             complement(10886..>11286)
                     /locus_tag="PK288_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPM18890.1"
                     /translation="RNMMAVGVKPSKIVLGTAFYGKGRVVKSTDNNGLYQIPVPVRRW
                     LGGGYSYLKDSVVNKKGFTRYWDAESKAPYLFNADQKAFITYDDEESTRIKCEYILKH
                     KLAGIMFWEYFSDRKHYLLEVINKSFGYRK"
BASE COUNT         3046 a         2414 c         2652 g         3174 t
ORIGIN      
        1 cagggcggcg ggtttctaca ataccctgct tgagggcgat gatcctgcga ttgtcatcga
       61 acctcttaac ggatacagga tcaaggaaaa aatgcccgac aatataggtg agctgaaact
      121 gcccctggga attcctgaag taattactga aggtgatgac ataaccattg taacctatgg
      181 ttctacggta aggattgcag cagaagctac agaacagtta tcaaaacatg gaatcagtgc
      241 agaactaatt gatgttcaga cgcttgtgcc gtttgatacc gaacggagga taattgaatc
      301 catcaggaag accaaccgta ttcttttcct tgatgaggat gtgcccggtg gtacaacagc
      361 atatatgctt caggaagttc ttgaaaaaca gggaggttgg aattatcttg acagcccgcc
      421 aaaaacactg actggtaaag aacacaggcc tgcttacaca actgatgggg attatttctc
      481 aaagccaaat gcagaggata tttttgatat ggtttatgca atgatgaaag aatcagttcc
      541 ggcaagatac tgacgacagc aatcagtcat tcgctgggag gattacgcgt atatgattga
      601 tccgttagcg gttgacttct tataatcctt ctgcctttga tccaataata gtgagtaatt
      661 ctttcgcagc ctccagggga cccgatgttt catggcccag gatcccgagt cgtgttctca
      721 tctggccgac gaagatattt ttgctgaaga aggattcgaa aaaccttttt gctatttttt
      781 catgaagctc cctgtattcg ggaggaccga agatatttgc aaagtaggaa tgcaccagtc
      841 ccgtcgaggt tgtagtgccc ttgctcatca cggcgccttc acggaaaaca tcgagcgccc
      901 aatcaacgtt gtcaaatttt tcaattacgg gatgatcggc gtcgatttcc tcataattgt
      961 tggcgtacag gatgtaatca attttatgac ccttgattat ggttgcaaaa ttggtggcag
     1021 gaataacgat tcttgcattg acctggcttg gattcataat aattgatctg tcgagatgac
     1081 cgtaggcata gccgggttgc aggtcgtcga gcctcagaaa agctccgatt tccgttccga
     1141 aacctaccgg atttccgtcg ttatcgagtt caatcgaacc catatcgtca gcgatgatcc
     1201 tgagttccct gatttccttt tcgccaagaa ccctgaaagc ttcaagtgtt tctgactttc
     1261 cggccccggt atccccgatc atcaggatag ttgcttcctt tctgcctttg agcaggattt
     1321 tcaccatagc tccatggaaa ggaagatttc ccgactgcat tattattgaa ttatgaagcg
     1381 tgaggaccat ttttttcaga tagccgaaat atccgaattc cagccggccc gggattgctc
     1441 ccacgaaaag attgttggct ttgtcgtgat ggaataccgt ggaatatttt gaaatgctgt
     1501 cgaatgtttc cgggggagcc ccgtatatga acactgcatc gggctttttt gcaagatctt
     1561 catcttctgc aacctggaaa aggttacaca ttgaaagccc aagctcaaga aaggttatat
     1621 ggaaataaat cattataacc agtttgccga cttttgcggg gtaacataac cagtcttcgg
     1681 gatcgagttt tatcaggtct atagggtttt gctggatcag cctgaaatca ccggtacgtt
     1741 tattcatcgg ctggtgaata acgagaggag gatagatcag gatctgtctt gtaacaggaa
     1801 tgctgttaag aaactgatac ttaggatccg gccatgttat ttcaaaaggc agtgaaatta
     1861 ccgccatctc tgcacctgca ggcacctgcc tgtaaacggt cggatggttt cccgtgatgt
     1921 tttcctgcac atgcctgtaa actgtgcgta tcaggtgggt caaatgttca atggtttcat
     1981 taaaggtacg ataaggtctt ttatcaagcc tgtctccttc cgaatcattt atgatgaacc
     2041 tgtcgaaatg ccgccagtaa tcgtaaagat attctacaaa accgctcagc agatgggtat
     2101 ctcggaggaa aacggacgat ttgtcaaaga tatgaggtac gacgcttcct tcatatttaa
     2161 acaatagtgt aagggtttta accagcgaag tgactgattc ttcatcaacc ttaccgtttt
     2221 caaaaatatc gagcaaagga gatcctatat cttccaggtg ttttatggaa tgatccagta
     2281 ttttcctgaa tttttcgctt gcaaggagtt catccgctgt ttcacacatc ctctcccgaa
     2341 tattcaggac gactttgttt cctacaatcc ttaatgtact cttttgcata atggttttaa
     2401 acaaaacgca caaaagtatt acaatatttc attgttatca tatgaggctg taaatttttt
     2461 gtatttcata caccttttat gatttcgatc aataaaattc gtaaattgta gcactaaagt
     2521 gtgatgtatg aaaccggaac tcgatctctt tgcgattcac ccggaagata agataccgga
     2581 gaaaggaaaa gtgctgattt cagagccttt tctggcggat acttttttta atcgcagcat
     2641 tgtttatctg accgatcaca atcaaaaagg atcggtaggt tttatcctca acaagaaact
     2701 ggatataaag atttgtgatg cagtaagcgg ttttgaaggt tgggatgaaa atcttaatat
     2761 gggcggtccg gttgcgcccg acacacttca ttatcttcac agtcttggca atctcatacc
     2821 gaaaagcgta cctgttgatg gtaccatata ttggggaggt gatatagaaa ccattaaagg
     2881 attgatcagg acaaatgcga tcaagcctta tcagatcaag tttttccttg gctattcagg
     2941 ctggtctgca gggcagctcg accgggaact gaaagagaat tcgtgggtga ttgccagggt
     3001 aaaatcggaa atagttatgt ctgcatgggg cagcgacagc tggaaaaggc tgctcaggtc
     3061 gtttaataac aagtacagga tatgggccga ttttccagag tcccctgaaa tgaactagat
     3121 atgtaatatt ttatatatca attctttaag taaatctccg gaatcagccc cggtatcacc
     3181 ccagcctttt gatttcatgt cgaaggtacg gagtagttca atcacccgta ccgtttcaga
     3241 tatgttgtac ctggctgcag cagtttcata atcctttacg aaaaacggat tgattttaag
     3301 cattgcagca acattgtttc tcgacttgtc ggctatataa tgataggtaa gtatcctgac
     3361 aaacaggctg aacagggatg ctatggtaag ggttacggga ttatctttct ggttttctga
     3421 gaaataattg attatccggt tggccctgag gatatttttt tcacctacgg ccttttgcag
     3481 ttcaaaattg ttgtactccc tgcttattcc tatatttttt tcaatcagtt cagttgttat
     3541 aacctgtttt ccggcaggaa gagtgatgag gagtttatcg agctcgttgg caatcttatg
     3601 caggtcggtt ccaagaaact cagtgagcat cgcggacgca aagggttcga ttcttatgcc
     3661 cctggtcata agatatcttt caatccatgg cggaacctgg ttatcataca aacgtttcga
     3721 ttcaaaataa atcccatggg tttccaatgc cttgtagaat tttgttcttt tatcaattgt
     3781 cttgtatttg taattgagca ccagaatggt ggaaagcaga ggtttttcaa gataaaagat
     3841 taggttttca attttcttga ctgactgggc ttccttaact attacgacct ggtggcttgc
     3901 catcattggg aaccgccttg ctgtatcaat tattgactct atggttgtat cttccccgta
     3961 aagaatcagc tggttgaaag ctttttcctg ttctgacaga accttgtctc cgataaaatc
     4021 ggtgataagg tcaatataat agggttcatc accggcgaga aagtagacgg gcctgaagat
     4081 cctttttgtc agatcggaga ttatgtcttc atatgttagg ggcatggtca gaatcttaga
     4141 tgttttaccg agatcccgtt attttttatt tcccttaaag cgtcaattcc aattttaaga
     4201 tgcatctcaa caaatttgtc tgtcacattt ttgtcgattg ctgaggtctt tatgcccgtg
     4261 gaaatcattg gctggtcgga aacaagcaat aaggcaccag tgggaatttc atttgcaaac
     4321 ccgacagtga atattgtggc tgtttccatg tcgattgcca ttgcccgggt tttgaccagg
     4381 tattttttaa aacggtcgtc atattcccat accctgcggt ttgttgtata aacagtacct
     4441 gtccagtatt ccttgttgtg cctggtaatt gatgctgata ttgcgctctg cagctggaat
     4501 gcgggcaggg cagggacttc caggggcagg tagtcgtttg aggtaccttc gcttctgatg
     4561 gcagcgatcg gaagtataag gtcgccaaga gtgtttttct tttttaggcc cccgcatttt
     4621 cccaggaaca ggaccgcctc gggttccacc gcactgacaa ggtccataac ggtagctgcg
     4681 ttgggacttc ccatcccgaa gcagatgatg gtaatgccat cagccgtgga gcttggccag
     4741 ttcttgtccc ggccttccac ttccgaattg ttgatctggg caaaaagctc aacatagtat
     4801 acgaaattgg tcagaagaaa atatttgccg aaagtatcca ggcttttacc tgtatatcgc
     4861 ggcagccagt tattgataat atcggctttg gtcttcattt tttttagatt atgaagttta
     4921 tctgaatata tttggaagtt taaaaatacg aaaagtaagt tgatacagct gaatcttccc
     4981 tcatattcat tcaggattac cggcagtgag ggttctgaga tgatatttga tccgatccgc
     5041 aggaaatatg tcaggcttac gcctgaggag tgggtcaggc agaattttgt ccagtatctg
     5101 attcatgaag gaaaataccc gccggggctg atggagactg aagtgatgtt caggttgcat
     5161 aacaagcttg tgaaaagaat tgacatcagg gtgcgggatc gcaggggaaa tcctgtgctg
     5221 ctggttgagt gcaagtccta caaggttgct ataaatgatg ctaaggtcag ggatcagatt
     5281 ggcgagtata acatgcattt caaggtgcca tatgtagtga tcacaaatgg cctggatcat
     5341 tatgcattca gatatgatca ggaaaggaat ctttatgatt tcatgatggt tatcccgttg
     5401 tatgaagatc ttctgacctg attcaggcta atttctcaag tatctgccag cactgataca
     5461 aacccctggg aacatggaaa catcctttcc attttccccc tttcagagga aggagcactt
     5521 ctcccctgcg gttcaggtaa ccgaaccatt ccccatgttc cctgtctttg aaatgatccc
     5581 aggtataatc atgaagtata tcaaaccatt ccaggttgtt cgcggtaccc gtaagcaggt
     5641 atcctttcag cagagtgatc agtgtttcga tgtgaaccca ccatagtttc tggtcccatt
     5701 caagctgttg tgggggataa ccaagcctgt ccatgaaata gagtataccg ccaaattccc
     5761 tgtcccatcc gtattcaatg gttctgaggg ctatttcgtt agcctttgta atcagttccg
     5821 gtctgttcat ccgttttcct aaatccatga tgaaccacat tgcctccagt gcatgccccg
     5881 ggttgaagag tcttccgtcg aaggtgtcgg acagagctcc gtcctcagtt acattttcga
     5941 caatcagacc cagctcaggc cggtaaaaaa tatccataac ttcgtgtatg catgtctcta
     6001 tggttttttc caggaattcc ctgtcaatca gctgctctat ttccagtgaa aggttgcaaa
     6061 ggatcattgg cagtgcaaaa ttcttaaggt tgcgtgtccc cggataagct ttattccagc
     6121 ggtctttggg attggctgcc cgtgacagga ttaaatcaaa ggttttcctg gcaatgtcgg
     6181 catactcctg tttccccgtg gcgaggctca gctgcccgaa cgccatggcg gcaaaggtat
     6241 aagagaaaat attgtaaggt tcaaccagag gtctgccgtc tcttgcaagg gaaaaatacc
     6301 agttgaaatt gccgtcatgg ccgtgttttt tcaggaattc acctccgtga atcgcgcact
     6361 caagccattc ctgttttctt tcaagcttgt tgaaaagcat tgagaacatc cagacctgcc
     6421 ttccctgaag ccagataaac ttatcagtat caaatacttt tccgtcccgg agaagacagg
     6481 tgaaataacc tcccatctcc ctgtcgattg aattgttcag ccagaaaggg agcacattgt
     6541 caagcagttc atccctgtac agggatgcca gtgatttaaa gtccatgata ttatttttgg
     6601 gttttatgtc cacatttttt caatctcctc gagtgatttg ccagttgttt caggcacctt
     6661 tttccagatg atccatatgt aggggaagca cataagggcg aacaacagga atgttcctga
     6721 gggtttcagg ttttcgagca gccatggtgt cagctgtccg ataagatacg tacctatcca
     6781 tagtgagaaa ccggcaattg acatggccag tcccctgacc ctcatgggat acatttctga
     6841 cagaagcacg aaaataacag cgcatattga gatggcacat gagaaaatat aaaacaggaa
     6901 aaagaccagc aggattaccg gggttagtcc ccattccttt ccaaaggcaa agtaaatggc
     6961 aatgcatatg agagaaatta tcatacctga aacaccccag taaaccagct gttttcttcc
     7021 taccttgtcg atgattataa gggccaggac agtggtcagc atgttgacaa ggcctacgag
     7081 aacctggtag aataatgaat ctccgcttga taatccgctg tcggtgaaaa tagaagggcc
     7141 gtaataaaga acggcattga cgcccatgaa ttgtcccagc atggctatgg caactcctat
     7201 caatactgcc ttcaggatcc cgggttctgc aaggaatttc cattctgatt ttttctcagt
     7261 ctgaacaacc tgctgtgtct gcgcaatttg gttattaacc tcatcgtccg ttgcatatat
     7321 ccgtttcagg attcttgagg ctttttccgg cgagtgcttc aggatcagcc atcgcggact
     7381 ttcgggtata aaaaagatca taaagaagaa catgacagcc ggtacggtct ccatgcccag
     7441 catggctctc catacttcac tgacaaaaat cttattcagg acaggattag cccaattacc
     7501 gccggctgcc agtttcagta actggaagtt ggcaagataa gcaccaagaa aaccgattgt
     7561 tattgccagc tgataaaatg atacaagcct gccccggtgc tttgcaacag atatctccga
     7621 tatatagatt ggtgatacaa ttgatgctac gccaatccct attccgccga caagcctgta
     7681 tgcaaccagc ccgttgatgg tgtttgaaag tgcacatcct atccctgtaa ccatgaaaag
     7741 tattgcggag atgatcattg tcttttttct tccgaggaag tcgctcatga ctcccgccac
     7801 tgcaactccc atgatcgatc cgaccagggc gcttccaaca taccatcctt cctgcagcgg
     7861 acttagattg aactggctgg atacctgggt tattgtacct gaaatcactg cggtatcata
     7921 accaaaaaga aagccgccaa gagcagctac aagggcaaga aaaactatat aacccataat
     7981 tatggcttgg ttgagaagtt cgttataaaa agatctgcca aaattcctga atcaagctga
     8041 atcaggaaaa tccgtaaatg ttaattttag catccgtaaa tggagtggat aaaattaaat
     8101 ccggtgataa aaataataca atattttaat tattcatgaa tagggtatcg tgtgcggtaa
     8161 tcaggaacga agagaatgaa atcctcatac ttcaggggga gaaggatgcc ggcgaccctt
     8221 tgagatggga attcccttat ggcagccccg cagaagacga gacggatgag gaatgcatta
     8281 taagggtaac aagggaacag cttggtattg atattgtcat ctgcagtctg atggaaatag
     8341 ttgagtatga ctgtggttac gggcagatca gcctgacccc gttcatatgc gatacacttg
     8401 atgaattgcc attcctgacc gttcataccg cattcagatg gataaccccg gatgaattac
     8461 ggaacattga attcacaggg tttaatgttc aggtagccca cgattatttt tcggatcata
     8521 tagggcaagg aaatcgctgc gaagaaactt tggccggtga gttcagcaac ctggacagtg
     8581 atgatgccat cagactgatg gtgggctctt ttaagggggc cagggaagct gaactgcttg
     8641 caggatcggc aatcgccaac ggagaggtct tgaaaaaact gctcgactat tcgttttctg
     8701 atgacagaaa actggcattc agggcatcct ggacattgtc aaagcttcat gataagatac
     8761 cggagattgt cacaccctat ctgggcagat tgataggagc gcttgatact attgataatg
     8821 agagtgtcca aagatctttt ctgaggattg tgtccctgag cgatcttaat cttgttccgg
     8881 ccagggatca gggaatgctg gccgatcatt gcttcaggat gctgaaatca ggattttcag
     8941 caattgccgt aaaggcttat tccattgaaa taatttataa actcgcactt atttaccccg
     9001 agctttccgg tgagttagcc gcaacggtga ggctgcttga ggatgaggct ccgacagggg
     9061 tcctggcgaa agggagaaac ctgctcagaa aactggctgc aaaacaggga gaagcagatc
     9121 cctcaggtat gaatatttat gactgaatct ttgtaatttt gcagctcaat ctgatcagat
     9181 gaaagtaatt gatacctcgg gcctgcaatg tcccgctcct cttattgcaa ccaggagggc
     9241 aatgaaggaa acagaagagg gagaatcttt cggtataatc actgacagta aatcttcctt
     9301 tgaaaatatt tcccggttcc ttaaagacca cgggcaggaa ttttcctgtg aagaatcttc
     9361 cggcaaatgg atcctgacga taaacaaggg gccgaccggc aatatatcag tgagtcctgc
     9421 cgggtattgt gaaaccccgg ttcctcattt ctccaaaggc gatttcatcg ttgcattcgg
     9481 atcagataaa atgggagaag gaaacgatga actcggacgt catctgatga tcaatttcgt
     9541 cctggctttg aaagatcttg acaagctgcc ggtcaggatg gttttttaca acagcggggt
     9601 cctgcttggg tcagaagatt cgaaggccgc agatcatctg aatgaaatgg aaaagatggg
     9661 cattgagctg ttgttttgtg aaacatgcgt aagttactat tcgctgagta aaaaaatgaa
     9721 ggtcggcaca ttgagcaata tgtttgagat agcacagatc atggcctcag cttcgaatat
     9781 tgtaaagccc tgaaatgatt tagcctgtat catgaaaatc gatcttcttg aaatggctga
     9841 cggcggaggg tgttcatcga agataccggc gctgcagctt gaggaatttc tgaaacatct
     9901 gcccttgcct gaggatccgg atatcctcgt aaatatcagt aatcatgatg atgccggggt
     9961 ataccggata aatgatgaac tggccctggt gtttacaaat gattttttcc cgccggtatg
    10021 cagtgatccg tatgaatttg gtcagatagc agccgtcaat gcgttgagcg atgtatatgc
    10081 gatgggaggg aggccggttg ttgcccttaa cataatgatg ttcccgtcat cccggctttc
    10141 acctgacgta tatgccggga tactcagggg cggatatgag actgcagccg gagcgggagt
    10201 gagcataata gggggacata ccattgatga tccggttccg aaatatggtc ttgcagtgat
    10261 tggctttgtt catccttcgg ggattataac gaatgcaggc gccatgccgg gcgattccat
    10321 aattcttacg aaacccctgg gaaccggtat tatcctggcg gcaaacaggc ttggccttgc
    10381 atcgcaggca gatatcagcg aggcactggc atcgatgaaa cagttgaaca aaccgggtgc
    10441 agatataatg aaaaagtacg gtgtgaaggg agccaccgat ataaccggat tcggtctggc
    10501 cggtcacacc ctgaagatgg ccagggcgag tgaagtatcg ataaccctga acatgaagca
    10561 ggtccgtctg attggaaaca cattggccct ggtggaagac ggatgtattc ccggtgcagc
    10621 tttcagaaat cttgattata ttgaaaaaga tacccgcttc tctgccgggc ttgcgtataa
    10681 ccttaaaatg gcagcctgtg atgcccagac ctcgggaggc ctgctgataa gcgctgcacc
    10741 ggaaattgct ggccaggttg tggatgagct ttccgaaacc ggattccaca aggctgcaat
    10801 aattgggaaa gttactgaaa gaagcgaaaa acttctttac ctcaatgaat agaatcaata
    10861 aaggcaacca aattgcagaa tgcagttatt tcctgtatcc gaacgacttg tttatcacct
    10921 ccagcagata gtgtttccgg tcgctgaagt actcccagaa catgatcccg gccagcttat
    10981 gcttcagtat atactcgcac tttatcctgg ttgattcctc atcatcgtag gtgatgaatg
    11041 ccttttggtc ggcattgaac aggtaagggg cctttgattc agcatcccag tagcgggtga
    11101 agcctttttt attgacaacg ctgtccttca ggtaggaata accgcccccc agccatctcc
    11161 tgaccgggac gggtatctgg tacaagccat tgttgtcggt gctctttaca accctgcctt
    11221 tcccgtagaa agctgtgcca aggacaatct ttgacggttt gacgcccaca gccatcatgt
    11281 tcctta
//
LOCUS       DAOOYG010000082        11250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6147_length_11250_cov_6.045377, whole genome shotgun sequence.
ACCESSION   DAOOYG010000082 DAOOYG010000000
VERSION     DAOOYG010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11250
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6147_length_11250_cov_6.045377"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PK288_07925"
     CDS             complement(<1..1115)
                     /locus_tag="PK288_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HPM18891.1"
                     /translation="MIRIIADDNIPFLKGILEPFSSISYLPGREITNQVVKGADALLI
                     RTRTICNEELLRDTSVKFIGTATIGYDHIDTGYCDKKGINWVSAPGCNSFSVQQYIAS
                     ALLRIAAENNFSLKDKTIGIVGVGNVGTKVEKFARTMGMNVLLNDPPRARQEKDIRFS
                     DLLTLLKESDIVTLHVPLSFNGEDRTFHLFSDEMFQLMKKESWFINTSRGEVVDTEAL
                     KGAIGNERIGGAVLDVWENEPELDIPLMHMAYLATPHIAGYSTDGKVNGTVAVVRKLS
                     SYFGLPLTDWSPEQIPLPRFQTIDMDCNGKGNEEIIRRSVLHTYNIVEDDVKLRFDPS
                     LFEKLRGTYPVRREFPAYTVKLRGCPSEAVTQLEELG"
     gene            1515..2417
                     /locus_tag="PK288_07930"
     CDS             1515..2417
                     /locus_tag="PK288_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18892.1"
                     /translation="MRLRFIFILVSLVPFLGTRAQTPITVLNDSLKIGNSNLPAISVT
                     IPEADYDKVLKAWIKELQSGTKSKVVTDNGEMSIFGARIKAVSPDPVNVYSILKKFDL
                     MLQLVVSFELKKDLYIESTGGAAEYAKATNYLKEFSKTQYIAVAKQEADLEQKKLRDL
                     EKELASLQKEKSKKLQKSIQSANSSILREQENITVQKNELSLVTSTLDAQNQELLAMQ
                     EDNPLRKEKAQSVKDLEKRQKKAVNSIESSEKKIKRAKSSIERAEAEIPRNERMQDKV
                     KEQIRLQEAVYRQYDEKLKKIRSY"
     gene            complement(2421..4559)
                     /locus_tag="PK288_07935"
     CDS             complement(2421..4559)
                     /locus_tag="PK288_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPM18893.1"
                     /translation="MMLVLSLLTVPAKTDEGMWIPILIEKYNISIMREKGFKLTAEDI
                     YSINKASMKDAVVRFGGGCTAEFISSEGLLITNHHCGYGTIQAHSSLEHDYLTDGFWA
                     MSAKDELPNPGLTVTLLKRMEDVTDRVLAGTTDEMANEKREGIILSNIKKIETEAMKN
                     NGYQANVRPFFMGNQYFLFVEETFRDVRLVGAPPSSIGRFGGDTDNWVWPRHTGDFSL
                     FRVYADRNNNPSDYAPDNVPYKPGFYFPISLRGLNEGDFTMVIGFPGTTYEYVPSYYI
                     DMIKNQVNPRLIDIRARKIEVIDEAMESDPLLRIKYSAKKAGISNSWKKWTGEILGLE
                     RMKTIEKKREFEDQISEWIKADSARNRKYGMIFQAYRDIYPLLSEYMLVNGITGEVMN
                     GIETVALARNLKGLSDLVESRGNSGDIESLKKRLIEYSGSFFRDYDRWTDEKLFVALL
                     KIYGEILEEKWQIPEYRKLMKMSKGDLGHFARKLYDSSVFTEEKEFRKFVEGFGLGSL
                     KKLKKDVFYNLAIDAAELVAKTIRPELGRFNAELQEHNRLYMKAQMEFQDDRIFYPDA
                     NSTLRVAYGKISGYYARDAVYLTHRTTLGGIIEKDNPEIYDYAVPGRLKELYASGDFG
                     RYGQDGQLNVCFIANNHTTGGNSGSPVLNANGHLIGINFDRGWEGVASDMAFNPDQSR
                     NISLDIRYALFVIDKFAGAGYLLGEMTIVE"
     gene            complement(4739..5338)
                     /locus_tag="PK288_07940"
     CDS             complement(4739..5338)
                     /locus_tag="PK288_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HPM18894.1"
                     /translation="MPLDEINRHTIILASGSPRRRQLLEDLGLRFEVVNHDWDEDFPD
                     DLKGAEIALYLAAKKAETLGGQLREGVIIITADTIVWCGNRVLDKPSDENEAMDIIRR
                     ISGNTHEVITGVCLLSSRGLRSFYSSTKVTFEELTEAEIEYYVRNFKPFDKAGAYGIQ
                     EWIGLAACSRIEGSYFNVMGLPVEKVYHELLSLVRSEGR"
     gene            complement(5340..6323)
                     /locus_tag="PK288_07945"
     CDS             complement(5340..6323)
                     /locus_tag="PK288_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HPM18895.1"
                     /translation="MKAFFKLIRWQNLLLIVLTMMLMRYAVIEPVISKLSVVLVHGQE
                     GEVPLMLKFPWYDFVILILATLLITAGGYVINDYFDIKTDLINKGKVIVGTAIPRKKA
                     MMWHNILNAAGVAAGFYISWKAGYFWLGTIFLIVSGLLYFYSASYKRQFLVGNLVVSV
                     LTAMVPMLVFIYEWPALYRYYSVHAMAPPDLSILFYWIGGFALFAFITTLTRELIKDI
                     EDFEGDLAYGRNTIPVVLGVLTSKMLSVCLIIITIVLLLLVWYFFLADRYTLIYISLF
                     IVLPLFYVIYQLIVSKGRKQLHIASTMMKLIMLAGVLYSVLVKYIITWNLV"
     gene            complement(6326..6847)
                     /locus_tag="PK288_07950"
     CDS             complement(6326..6847)
                     /locus_tag="PK288_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPM18896.1"
                     /translation="MNNFKQALTRIKAFVFDIDGVLSLQTIALNVFGTPNRTVNLRDG
                     YALQLAVKKGYHVGIISGSNSKEYLKRLKYLGIKDVYLNSRSKTDHFREFLKKHNLHE
                     SEVMYMGDDIPDLNVMKEAGVPVCPADADIEIKQASQYISDRKGGEGCVRDVIEQVLR
                     LHDKWLDDDAYSW"
     gene            complement(6840..7628)
                     /locus_tag="PK288_07955"
     CDS             complement(6840..7628)
                     /locus_tag="PK288_07955"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPM18897.1"
                     /translation="MKKTGISFIGAGKVAGAFCSELFNSGHRIINIVAETEDSCRRLA
                     ESCNASWSLDTEFKEDTDLILIAVPDDKIESVAAGIKCNEKTVVAHMAGSVGLDVFHQ
                     KYRHVGVIYPLQTFSAGRNIEFSKIPFFIEGSDKDTITLLCGLVESLGAKAYFADAEG
                     RRLLHLSAVFVNNFVNHMLVSGKAIASMAGFQFEVLVPLLNETILKAVTNGPENSQTG
                     PAVRSDQGTIKSHIKLLSFSPDLQNIYREVTGSIMRFQKKNINE"
     gene            complement(8047..8364)
                     /locus_tag="PK288_07960"
     CDS             complement(8047..8364)
                     /locus_tag="PK288_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPM18898.1"
                     /translation="MSDQKNPNQINIELNEETAQGIYSNLAVITHSSSEFVVDFVRIM
                     PGIPKAQVKSRIILTPEHAKRLVAALQDNISKYESVHGIIKEVKGSAPVLPLTFGGPT
                     AQA"
     gene            complement(8382..>11250)
                     /locus_tag="PK288_07965"
     CDS             complement(8382..>11250)
                     /locus_tag="PK288_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPM18899.1"
                     /translation="QMAVHLPLGNGAILEAQMLMLASHNILNPANGAPITVPSQDMVL
                     GLYYITKGKRSTATEKVKGEGLIFYSPEEVNIAYNEGKVDLHAFIKVKVNDRVDDKLV
                     NHLIETTVGRVIFNQYVPEEVGYINEILTKKSLRDIIGHVLKLAGTAKTAAFLDAIKN
                     LGFYSAFKGGLSFNLDDVIIPEEKTKFVTEGYEQVEEVLNNHNMGFITNNERYNQIID
                     IWTHVNARLTQSLMKQLSADKQGFNSVYMMLDSGARGSKEQIRQLSGMRGLMAKPQKS
                     GATGSEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVSQ
                     DVIINEEDCGTLRGLVAVAIKKNEEIVESLYERILGRTTVHNVYHPLTGELIVESGQE
                     LTEELAKKIEDSPIEQVEIRSVLTCESKRGVCAKCYGRNLATGRMVQKGEAVGVIAAQ
                     SIGEPGTQLTLRTFHVGGTASNITTESRLVARYSGIAEIDELRTVPKKDTEKGEIDIV
                     IGRLAELRIIDKKTGIALTTHTVPYGSRLYIKPGEEVEKGKLICEWDPFNAVIIAEVA
                     GKIDYDYLIEGVTFREESDEQTGFKEKVIIESRDKTKNPAIRITSVNSQEPRVYNLPV
                     GAHLIVETNRMVEAGEVLAKIPRAVGKSGDITGGLPRVTELFEARNPSNPAIVSEIDG
                     EVTFGKIKRGNREISIKSKTDEIKKYLVPLSKQILVQENDYVKAGIPLSDGAITPSDI
                     LAIKGPTKVQEYIVNEIQEVYRLQGVKINDKHFEIIVRQMMRKVEIVDPGDTKFLERQ
                     VIDKLDFMDENDWIYGKKYIVDSGDSQTLRPGMIVTARKLRDENSMLKRRDLKTVEAR
                     DAVPATSNQVLQGITRAALQTNSFFSAASFQETTKVLNEAAILGKVDKLEGLKENVIV
                     GHLIPAGTGQREYNSIIVGSLEEYESFTGNVPVATEAEPGK"
BASE COUNT         3044 a         2714 c         2225 g         3267 t
ORIGIN      
        1 aacccgagct cctcaagctg agtgacggct tcagaggggc atcccctcag tttcacagta
       61 taggcaggga attcccttct caccggatag gtacccctta acttctcgaa cagggaaggg
      121 tcaaaccgaa gtttcacatc atcctccaca atattgtaag tatggagcac cgatcttcgt
      181 attatttctt cattcccctt gccgttacag tccatgtcga tggtctggaa ccggggtaag
      241 ggaatctgtt caggcgacca gtcagtcagt ggcaatccga agtaagaact cagtttcctg
      301 acaaccgcca cagttccatt gacttttccg tcagttgaat aacctgctat atgaggagtt
      361 gcaagatatg ccatgtgcat aagcggtata tccaactccg gctcgttttc ccaaacgtcg
      421 agaactgctc ctcctattct ttcattgccg attgctccct ttaaggcctc ggtgtcaaca
      481 acttccccac gcgatgtgtt gatgaaccag ctttctttct tcatcagctg aaacatctca
      541 tccgagaaca aatgaaaagt cctgtcttcg ccattaaaac taagaggcac atgcagggta
      601 acaatatcag attctttaag cagtgtcaac aggtcggaaa aacggatatc tttttcctgc
      661 ctggcccgtg gagggtcatt gagcagcaca ttcatcccca tggtcctggc aaacttctca
      721 acctttgtac ctacatttcc cacacctacg attccgatgg tcttatcttt caggctaaaa
      781 ttattttccg ctgcgatcct cagaagagca gaggcaatat actgctgaac tgagaaggaa
      841 ttgcacccag gtgcactgac ccaatttatt ccctttttgt cacaataacc tgtatcaatg
      901 tgatcatatc cgatagtcgc ggtcccgatg aatttcacac tggtatccct gagaagttct
      961 tcattgcata tggtacgggt cctgataaga agtgcatccg caccttttac cacctgattt
     1021 gtgatttccc ttccgggtaa ataggaaatg cttgaaaatg gttcaagaat acctttcaga
     1081 aaaggtatgt tgtcatcagc tattatcctt atcatcagat ctttaatata tattgaaggc
     1141 cagggatgtt ccggaccttg aatttccgcg gtttttactg aaagatataa cggaacaaac
     1201 acgcggaata ttttaggcta aaaaaataat gaaagagcta gttataagta ataaacctta
     1261 ggaggaaatg ccatttcagc atcttgtttt tccaaccggc tatttatcgg cctatttgct
     1321 taaagataca tcttactgtt atcagtttaa cagtcacacc atatcaggat gtgatccagg
     1381 tcctgaacaa aataatccga tcaaacaaaa ggttctgaac tggcattggt tttttaatat
     1441 taacgacaga aggtaatatg attaattatt tatatattta tatcaaacaa aaaagactaa
     1501 aaaatccatt tgccatgaga ttaaggttta tcttcatttt agtttcttta gtaccctttt
     1561 tgggaaccag ggcccagaca cccattacag tccttaatga cagcctgaag atagggaaca
     1621 gcaatctgcc ggcaatatcc gttacaattc cggaagctga ctacgacaag gttctgaaag
     1681 cctggatcaa ggaacttcaa tccgggacca aatcaaaagt ggtgaccgat aacggtgaaa
     1741 tgtcaatttt cggggcccgg ataaaagcag tgagtcctga cccggttaat gtttacagta
     1801 tactaaagaa atttgatctt atgctgcagc tcgtagtctc atttgaactg aaaaaggatc
     1861 tttatattga aagcacggga ggtgcagcag aatatgcaaa agccaccaat tatctgaaag
     1921 aattctctaa aacccaatac attgccgtgg caaagcagga ggctgatctg gaacaaaaaa
     1981 agcttcgtga tcttgaaaag gagctcgcat cgcttcaaaa ggagaaatcc aagaagcttc
     2041 agaaatcaat tcaatcggct aacagtagta tcctcaggga gcaggagaac atcacggtac
     2101 agaaaaatga actaagcctt gtgaccagta cccttgatgc tcagaaccag gaactcctgg
     2161 ccatgcagga agataatccc ctcagaaagg aaaaggccca gtcggttaag gatctggaaa
     2221 aaaggcaaaa aaaggcagtg aattccatag aatcatccga gaaaaaaatc aaaagagcca
     2281 agtccagcat agaaagggca gaagctgaaa ttccgcgcaa tgaaaggatg caggacaagg
     2341 ttaaggaaca gataagattg caggaagcag tataccggca gtacgacgaa aagctgaaaa
     2401 aaatcaggtc atactgatca tcattcaacg attgtcattt ctcccagcag gtaaccagct
     2461 ccggcaaatt tatcaataac gaacagcgca tatctgatat cgaggcttat attacggctc
     2521 tgatcaggat taaaggccat gtcgctggca accccctccc atcccctgtc aaagtttatt
     2581 cctatcagat gtccgttggc atttaatacg ggacttcccg agtttccgcc tgttgtgtgg
     2641 ttatttgcaa tgaaacagac atttagctgt ccatcctgcc cataacgtcc gaaatctccg
     2701 gatgcataca attccttgag ccttccgggc acagcataat cataaatttc aggattatct
     2761 ttttcaatta tgccgccaag ggtagttcta tgggttaaat aaaccgcatc ccttgcataa
     2821 tagcctgata ttttcccgta agcaaccctc agggttgaat tggcatcggg ataaaagatc
     2881 ctgtcatcct ggaactccat ctgggctttc atgtaaagcc tgttatgctc ctgcaattcg
     2941 gcattaaacc tgccaagttc cggtctgatt gtttttgcga ccagctccgc agcatcaatt
     3001 gcaagattat aaaacacatc ctttttcagc tttttcagag aacccagacc gaagccttca
     3061 acgaatttcc tgaattcctt ttcttccgta aaaaccgaag agtcatacaa tttgcgggca
     3121 aagtgcccca aatcgccttt ggacattttc atcaatttcc ggtattcggg aatctgccat
     3181 ttctcctcaa gaatttcacc atatattttc aacaaggcaa cgaatagttt ttcatcggtc
     3241 cacctgtcat aatccctgaa aaaagatcct gaatattcaa tcaatctctt tttcaggctt
     3301 tcaatatcac cggaattgcc cctggattcg acaagatccg aaagaccttt gagattcctg
     3361 gccagggcaa cagtttcaat gccattcatt acttcaccgg taatgccatt cacaagcata
     3421 tactcgctga gaagaggata tatgtcgcgg taagcctgaa aaatcattcc atatttcctg
     3481 tttctggcac tgtcagcttt tatccactca gatatttgat cctcgaattc ccgttttttc
     3541 tcaattgttt tcattctttc gagccccagg atctctccgg tccatttttt ccaggagttt
     3601 gagatacccg ccttttttgc agagtatttt atcctcagga gaggatctga ttccatcgct
     3661 tcatcgatca cttctatctt cctggccctg atatctatca gcctgggatt gacttgattc
     3721 ttaatcatat caatataata agacggcaca tactcgtaag ttgtgccggg gaagccaatg
     3781 accatcgtaa aatcaccttc attcagacca cgcagagata tcgggaaata aaaacctggt
     3841 ttgtacggaa cattatcggg ggcataatcg gatggattat tgttcctgtc agcatagacc
     3901 cggaagagcg agaaatcacc tgtatgccgc ggccacaccc aattgtccgt atctccccca
     3961 aacctgccga ttgaggaggg aggtgcgcct acaagcctta cgtccctgaa ggtttcttca
     4021 acaaacagga aatactggtt gcccatgaaa aaaggcctga cgtttgcctg ataaccatta
     4081 ttcttcatgg cctccgtttc aatctttttt atatttgata ggataatccc ctctcttttc
     4141 tcatttgcca tttcatcagt tgttccagca agaaccctgt cggtaacatc ttccattctc
     4201 ttcaggagag taacggtaag accgggattg ggtaattcat cctttgccga catagcccag
     4261 aatccatcgg tgagataatc gtgctcaaga ctggaatgag cctggatcgt accgtaaccg
     4321 caatgatgat tagtaatcag gagaccttct gaagagatga attctgccgt acatccgcca
     4381 ccaaacctga caaccgcatc tttcattgat gctttgttta tgctgtaaat atcttctgcg
     4441 gttaatttaa atcctttttc acgcataata ctgatattgt atttttcaat cagtatggga
     4501 atccacatgc cttcgtcagt ctttgccgga actgtcagca acgacaagac cagcatcatt
     4561 ccaggtaaat ttaaatatct catattgtga gttctataat cctttatcat ttctaaccgg
     4621 cattttttct gatcatcacg ccaggttcac tgaaacctga tgtgccaata tttgtcagtc
     4681 aaacacgtac cggatcgttt cttttcaata tgcatccaga tctgccgggc ttacgcatct
     4741 atctcccctc cgatctgacc aatgacagca gctcatggta aaccttttca acagggagac
     4801 ccataacatt aaaataagag ccttcaatcc ggctgcaggc agcaagtccg atccattcct
     4861 ggatcccata tgccccggct ttgtcaaatg gcttaaagtt ccggacatag tattcaatct
     4921 ccgcctctgt gagctcttca aatgtaactt ttgtcgagga gtaaaaagat cttaatccac
     4981 gggaagacag aaggcagaca ccggttatta cctcatgggt gtttcctgag atcctgcgaa
     5041 ttatgtccat tgcctcattt tcatccgacg gtttgtccaa aaccctgttg ccacaccata
     5101 ctatggtgtc ggctgttatt attataacac cttcccgaag ctgcccgccc aatgtctcgg
     5161 ctttttttgc ggcaagatac aaggctattt ccgctccctt taaatcgtcg ggaaaatctt
     5221 catcccagtc atggttcaca acctcaaatc ttaatccgag atcctccaga agctgcctgc
     5281 gcctgggtga gcctgaagcc agtattatcg tgtggcgatt gatttcatcc aggggcatat
     5341 cagaccagat tccaggttat tatatatttt accagtaccg aatataaaac cccggccagc
     5401 atgatcagct tcatcatcgt gctggcaata tgaagttgtt ttctgccctt gctgacaatg
     5461 agctggtaga tcacgtaaaa tagcgggagt acaataaaaa gagaaatata aattagtgta
     5521 tacctgtcgg ccaggaaaaa ataccagaca agcagaagca ggactattgt tataattatc
     5581 aggcataccg acagcatttt ggaagtgagc acacccagta ccacgggaat agtatttcgt
     5641 ccgtatgcca aatctccttc aaaatcttct atatctttaa ttaactcacg ggtaagagtg
     5701 gtaataaaag cgaacaatgc aaatcctccg atccagtaaa ataatattga aagatcgggc
     5761 ggggccatcg catgtacact gtagtaccta tataatgccg gccattcata aataaacaca
     5821 agcatcggga ccatggcagt aaggactgaa acaaccaggt ttcccacaag gaactgtctc
     5881 ttatagcttg ccgagtaaaa atacagcaag cccgaaacta tcaggaatat cgttccaagc
     5941 cagaaatacc cggccttcca tgagatataa aatccggcag caacacctgc tgcattaagg
     6001 atattatgcc acatcatggc tttttttctt ggtattgctg ttcccacaat tactttcccc
     6061 ttgtttatca ggtcagtctt gatgtcaaaa taatcattta tcacgtaacc ccctgctgtt
     6121 atcaacaaag ttgccagaat caggattaca aagtcgtacc agggaaattt aagcatcagg
     6181 ggaacctctc cttcctgtcc atgcacaaga acgacggaca gtttgctgat caccggttca
     6241 atgactgcat atctcatcag catcattgtg aggacaataa gcaggagatt ctgccatctg
     6301 atcagcttaa aaaacgcttt catggctacc aggaataagc atcgtcatca agccatttat
     6361 catgcagcct cagaacctgt tcaatcacat ccctcacgca gccctcaccg cctttcctgt
     6421 cagaaatata ctgggaggcc tgttttattt caatgtcagc atcagcagga cacaccggga
     6481 ccccggcttc tttcatcaca ttcagatctg gaatgtcatc tcccatatac atcacttccg
     6541 attcatgaag gttgtgcttc tttagaaatt ctctgaaatg gtcagtcttg gagcggctgt
     6601 taaggtaaac atcctttatt cccagatatt tcagcctttt aagatattct tttgaattgc
     6661 ttccagatat aatccccaca tgataccctt tttttacagc aagctgaagg gcatatccat
     6721 cgcgcagatt aaccgtcctg ttcggtgttc cgaaaacgtt cagggcaatc gtctgcagag
     6781 acagaacgcc atcaatatca aaaacaaatg cttttatccg ggtcagggct tgcttgaaat
     6841 tattcattta tgttcttttt ttgaaatctc atgattgatc ctgttacttc cctgtaaata
     6901 ttttgcagat caggtgagaa agataataat ttaatatgac ttttgatagt gccctgatca
     6961 gacctgacag ccggtcctgt ctgggagttt tcgggcccgt tcgttacagc tttcaggatt
     7021 gtctcattga gaagcggaac gagtacctca aactgaaatc ctgccatgga ggcaatcgct
     7081 ttgccagaaa cgagcatgtg gtttacaaag ttattcacaa aaacagccga caggtgaaga
     7141 agcctcctgc cctccgcatc ggcaaaataa gcctttgccc ccaatgactc aacaagtccg
     7201 cacaagaggg taattgtatc cttatcgctt ccctcaatga aaaacggaat cttgctgaat
     7261 tcgatgttcc ttcctgccga aaaagtctga agcggataaa ttactccaac atgcctgtat
     7321 ttctgatgaa acacatcaag gcccacgctt ccggccatgt gtgctacaac tgttttttcg
     7381 ttgcatttaa tgccggcagc aaccgattct atcttatcat cgggtactgc gatcaatatc
     7441 agatcagtgt cttccttaaa ttcagtatcc aacgaccatg atgcattgca ggattcagcc
     7501 aggcgtcggc agctatcctc agtttcagct acgatattta ttatcctgtg accagaatta
     7561 aaaagctccg aacaaaatgc cccggcaact ttaccggctc cgatgaatga tattcctgtt
     7621 tttttcatgt aaaaataaat ttatatactt ctgaagatta agtgattcac atggaaccca
     7681 catgagtttt atttccatgc ttgtttgtga tcttgtatca tgtgggttca tacttgtgat
     7741 tttttaaaag tttgactgcc gtctgtccac agggtagtga accaaatatg attgaacacg
     7801 gtaatattca ttcatgctgg tgttcctggt cattcatacg gatttcatga gtcctgaatt
     7861 tatattttat tgaaatgatg cctcccgggc ctgtcggacc ggtaattaat atgccttacg
     7921 gtgttgtgaa caattattta tggattcagc acatgcggga ttcatcagat atctgacaga
     7981 cacgaaatga ttgaaaaaaa tgaaggtgtc cttgtgagac accctcttat gatcatgtaa
     8041 tgcatttcaa gcctgtgcag tcggcccccc gaatgtaaga ggaagaaccg gggccgaccc
     8101 cttgacttcc ttgattattc catgcacgga ttcatacttg gatatattgt cctgaagagc
     8161 ggcaaccagc cgttttgcat gttcgggggt cagaatgatc ctcgatttaa cctgtgcctt
     8221 gggtattccc ggcattatcc ttacaaaatc cacaacaaac tctgaagaag aatgagtaat
     8281 tactgcgaga tttgaatata tcccctgggc tgtttcttca ttcaactcaa tgttgatctg
     8341 attagggttc ttctgatctg acataagctg ttttttggtt attatttgcc tggttctgct
     8401 tcagttgcaa caggaacatt gccagtaaag ctttcatatt cctcgagcga tcctacgatt
     8461 atgctgttgt attcacgctg accggttcct gcaggtatca gatgtccgac tatcacattc
     8521 tccttaaggc cttcaagctt atctaccttt ccgagaattg cagcctcatt caatactttt
     8581 gtagtttcct ggaaagatgc tgcagagaag aaactgtttg tctgaagcgc agccctggta
     8641 ataccctgca atacctggtt ggatgtggca ggaactgcat cccgtgcctc aacagtcttc
     8701 aggtcacggc gtttaagcat tgaattctcg tccctcagtt tgcgggcagt aacaatcata
     8761 ccgggcctca gggtctggga atcacctgaa tctacaatat actttttccc gtagatccag
     8821 tcattctcat ccatgaagtc gagtttgtca atcacctggc gttcaaggaa tttagtgtca
     8881 cccggatcaa caatctcaac cttccgcatc atttgcctga caatgatctc gaaatgtttg
     8941 tcatttatct taacaccctg caggcgatat acctcctgta tctcattaac tatatattcc
     9001 tgaaccttgg ttggcccctt gattgcaaga atgtcggatg gagttatcgc accgtcggaa
     9061 agaggtatgc cagccttcac ataatcattc tcctgaacca gtatctgttt tgatagggga
     9121 accaggtatt ttttaatttc atcggttttt gatttgatgg aaatctccct gttgcctctc
     9181 ttaatcttcc cgaaagtaac ctcaccgtca atttctgaaa caatggccgg gttggaagga
     9241 ttacgtgcct caaataattc tgtcaccctg ggtaatccgc ctgtaatgtc gcctgattta
     9301 ccaactgccc ttgggatctt tgctagcact tcgccagctt cgaccatcct gttcgtttca
     9361 acaataagat gggcgcctac gggaaggtta tataccctgg gttcctgact attcacgctt
     9421 gttatcctga ttgcagggtt cttggtttta tccctgcttt cgatgattac tttttccttg
     9481 aagcctgtct gctcgtccga ttcctcccgg aacgttaccc cttcaatcag ataatcatag
     9541 tctatttttc ctgcaacctc ggcaataatt acagcattga aaggatccca ctcgcagatg
     9601 agtttccctt tttccacttc ctctccgggt ttgatgtaaa gtcttgaacc ataaggaacg
     9661 gtatgggttg taagtgcaat tcctgttttc ttgtcaatta tcctgagttc tgcgagcctt
     9721 ccgataacaa tgtcgatttc gcctttttca gtatcctttt tcggtacggt tctcagctca
     9781 tcgatctcgg caattccgct atagcgggct acaagccgcg attcagtggt tatgtttgac
     9841 gccgttccgc caacgtggaa agtacgaagt gtaagctggg tgccgggttc gcctatactc
     9901 tgggcagcga taactcccac cgcctcgcct ttctggacca tccttcctgt agccaggttc
     9961 cttccataac attttgcaca tacccctctc ttcgattcgc aagtcagcac tgaacgtatc
    10021 tccacctgtt cgattggaga atcttcaatt ttctttgcca gctcctctgt aagttcctgg
    10081 cctgattcta caatgagctc acctgtaagc gggtgatata cattatgtac ggttgtacgg
    10141 cccagtatcc tttcatagag cgattcgacg atctcttcat ttttctttat tgcaactgca
    10201 acaagacctc tcaatgttcc gcagtcctcc tcattgatga tcacatcctg ggaaacatcc
    10261 acgaggcgtc tggtaaggta tcctgcatca gctgttttga gcgcggtatc agccagcccc
    10321 ttacgtgccc catgtgttga tataaagtat tcaagaacgg aaagtccttc cttaaaattc
    10381 gaaaggatcg ggttttcaat gatctccgaa cctgtcgctc ccgacttctg cggtttcgcc
    10441 atgagccccc tcatccccga caactggcgt atctgttcct ttgagcccct tgcccccgaa
    10501 tcgagcatca tgtacactga attaaagccc tgcttatcgg cagagagctg tttcatcagc
    10561 gactgggtaa gccttgcatt aacatgtgtc cagatgtcaa ttatctgatt gtatcgctca
    10621 ttattggtaa taaatcccat attatggtta ttcaatacct cctcaacctg ctcataacct
    10681 tcggtaacga actttgtctt ttcctcgggt ataatcacat catcaaggtt gaaagagaga
    10741 cctcccttga atgctgaata aaatccaaga ttcttgatcg catcaaggaa cgcggcagtt
    10801 tttgccgttc cggcaagctt gagtacatgc ccgataatat ccctcagaga tttctttgtc
    10861 aggatctcat taatatatcc cacttcctca ggtacatact gattgaatat caccctgccg
    10921 accgtggttt caatcagatg gttcacaagt ttgtcatcaa ccctgtcgtt gaccttcact
    10981 ttaataaagg catgaagatc aaccttgcct tcgttatatg ctatattgac ttcttcaggt
    11041 gagtagaaaa ttagcccttc acccttaact ttctcagttg ccgtgcttct ttttccctta
    11101 gtgatgtaat aaaggcccag aaccatgtct tgtgaaggaa ccgttatcgg ggccccattt
    11161 gcaggattca gtatgttgtg cgaggcgagc atcagcatct gtgcttctag gatggcacca
    11221 tttcccaggg gaagatgaac tgccatctgg
//
LOCUS       DAOOYG010000083        11248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6148_length_11248_cov_7.055928, whole genome shotgun sequence.
ACCESSION   DAOOYG010000083 DAOOYG010000000
VERSION     DAOOYG010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6148_length_11248_cov_7.055928"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="PK288_07970"
     CDS             complement(<1..1375)
                     /locus_tag="PK288_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPM18900.1"
                     /translation="MDKNTILQFLKDVELFTNLTADELQEVAGNAKEKHYNAGATLFH
                     ENDPREDVFIIYEGDVELFKQDSYGVEVRLALFGKGDFLGEGIWDHKTTHSTSARILE
                     KATLLVVNREFLNYNAETTLKILANITRVISRRMRNVNNRMLNLSTQYESGRTRSEQD
                     LLGVREVPYEAYYGIQTLRAAENFNITGVTLNFFPSIIEALSMVKEAAAKANFELGLL
                     DKKIKDAIVQACHELQNGKYQNQFVVDMIQGGAGTSTNMNANEVIANRAQEILGHEKG
                     SYSVCHPNNHVNMSQSTNDAYPTAVKIALFNSNKRIVSVLNTLIESFRKKGHEFSGVI
                     KMGRTQLQDAVPMTLGQEFEAYAVTLGEEVERLNQNAKLFLEINMGATAIGTGITAEP
                     GYSEIVIRHLRKITGLNLVLASNLVEATQDTGSFVMYSSAIKRLAVKLSKICNDLRLL
                     SSGPRTGI"
     gene            2030..2701
                     /locus_tag="PK288_07975"
     CDS             2030..2701
                     /locus_tag="PK288_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18901.1"
                     /translation="MEAHSVKIKSIGHITRDVLQIQTERPPELNFTPGQAIDIAIDKK
                     NWQEEKRPFTFTSLPDDDFLQFTIKTYPSRNGVTNQLLHLKRNDRLLLYDVFGAIEYR
                     KEGYFIAGGAGITPFISIFKYLGSVNAIGNNKLIYANKTNDDIILKYEFEYLLGKNFI
                     NILSDEKVEGYAYGYITRDFLEKQIADFGKNIYLCGPPPMMDAVEKILSDLNVDKSLI
                     VKEAD"
     gene            2821..3009
                     /locus_tag="PK288_07980"
     CDS             2821..3009
                     /locus_tag="PK288_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPM18902.1"
                     /translation="MAISKVWIEDGCTSCGLCEDICPEVFKVEDIATVKEGVNFNDFE
                     EGIKDAADSCPVEVIKYE"
     gene            3073..3501
                     /locus_tag="PK288_07985"
     CDS             3073..3501
                     /locus_tag="PK288_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HPM18903.1"
                     /translation="MATSKAQAHWKGKIKEGRGTMKFTGYEGTYTFASRFENAGGTNP
                     EELVGAAHAGCYSMYLSLLLTEEGLDPESIETTAEVTIDKDDSGPHIKDIKLHCKVKC
                     SGLSEGRLKELAAVTKEKCPVSRLYAGGTAKISAEALLIK"
     gene            complement(3591..4247)
                     /gene="pdxH"
                     /locus_tag="PK288_07990"
     CDS             complement(3591..4247)
                     /gene="pdxH"
                     /locus_tag="PK288_07990"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HPM18904.1"
                     /translation="MKSVRKKIVSSRSEFISDGLNEEELSADPIDQFEKWMDEAMRED
                     PEMANAVHLATTGPDGRPSGRIVLLRGFDQRGFVFYTNYDSRKGSDLKINNNASMTFF
                     WSKSYRQVRIEGTVFKLPPGESDSYFSSRPRESNISALASRQSRPLENRRILEERVRE
                     LENMLKDQTIKRPDNWGGYCLSPLYIEFWQGMTHRLHDRIFYRRENTNTPWTRGRLYP
                     "
     gene            complement(4388..5713)
                     /locus_tag="PK288_07995"
     CDS             complement(4388..5713)
                     /locus_tag="PK288_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPM18905.1"
                     /translation="MAELKDNSRKRISKPLKDNEGISRREALKTIGVMSAGISFLKIP
                     ENEICPPAFNAGLRPDALRQDPILAEAISQLEYLTPAKKFIVQRRGNPVLPELPEELL
                     PSIGLSQETWRLEIISDPESDSELGNPFTIENNNALTWSGLMKMAEKHAVSYLHVLSC
                     TNAPKPYGMGLWEGVPLREVLWQTKPGRNIRRIRFSGYHNNDPKQIFQSSLPINRVLE
                     EIPGELPVILAYKLNGQYFSHANGGPVRLFVPGFYGNRSIKWLQKIVLTNNHQANDTY
                     ADWNNDVESPVKTCAKFIKVPAEVRTKQMFAFTGMAQVGLLGLSKVQYCIMPADEPPD
                     ANDPYLALADWRDAMILPPPANWGSDLPGGKLPGNLMQIDSKTGKLVSWPLPNTIVHW
                     AASAKIDIPGKYDLRCRTIDANGTAQPMPRPFGRSGVNAIEAAKITANS"
     gene            complement(5990..6865)
                     /locus_tag="PK288_08000"
     CDS             complement(5990..6865)
                     /locus_tag="PK288_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPM18906.1"
                     /translation="MFRTIIDNVRTYAVITFGLMLMSVAWTAFLLPHRIAGSGVSGIG
                     AIVFYATGIPMGYTYLAINVILVIAGLRILGTGFGVKTIYGIVLSAIMLTVFQSLIPT
                     AVVQEKFMSAIIGGILCGTGLAIVFSQGGSTGGTDIIALIIIKYRNYSPGRIILLCDV
                     FIISSSYVVLTHLDSAQRVETMVYGFVTMAIQAYTLDAILSGYRQSVQIFIFSKHYEA
                     IADQITSRMRRGVTVIDGTGWYTKDSQKIIITLVRKNETSDVYRIIKSIDDKAFITVA
                     SVMGVYGKGFDVLKK"
     gene            complement(7074..7703)
                     /locus_tag="PK288_08005"
     CDS             complement(7074..7703)
                     /locus_tag="PK288_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18907.1"
                     /translation="MKKSILIAIMVFAISLNAHSQFSFGVAPGIATNSAYFGYKAGKV
                     VPWIGLQAANGGFKNEYKDHYYNGSEWVTDVSSRSVNLNLLMPSVGVKLFAVEKGGLK
                     AYFNLSGSKPMISGKMKEDGDVNEDFSEDLKKIKLFGAELGFGAEYFFSNNFSIGGEY
                     AIRFLGGNYTDRHEYEYWGETAVETDKFCARIAPTIAKITLNFYFGGGE"
     gene            8247..9041
                     /locus_tag="PK288_08010"
     CDS             8247..9041
                     /locus_tag="PK288_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM18908.1"
                     /translation="MDLIFCSLSSGSSGNCYYLGNRGHGILIDAGISATSIRNYLRTI
                     NVSMRSIDGVVVTHNHSDHIRGLEVFTRKNSIPAYATMKVRESILAAEKKISPGYIKE
                     IPMRQAFSLAGFEIEVFPVCHDAPETIGLHISAGTKKITIATDLGHICQTSAPYIKAA
                     NLLVIESNYDEQMLLNGKYPQFLKARIKSDHGHLGNHQAASFLAEMAGDNLENICLAH
                     LSLNNNTPELALNTLYKAFSEKGIDLNGHPRILVLERNRPSEMIKL"
     gene            complement(9124..9639)
                     /locus_tag="PK288_08015"
     CDS             complement(9124..9639)
                     /locus_tag="PK288_08015"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPM18909.1"
                     /translation="MKRLDTLFHDTESLILFGSIIIVTILVSVIIDKGLMNKLVSRSK
                     AAGNDITSIVFIKNIIRLTIYLFGVGWAFLSLPITKSFAHSLFAGAGVTTLIMGFSAQ
                     QILSNLMSGMFLILNRPFRIHDTIEVQDFRGKVVRITWHDTVIENEENSRIFVPNSVI
                     TSNIIKVISVK"
     gene            complement(9710..10771)
                     /locus_tag="PK288_08020"
     CDS             complement(9710..10771)
                     /locus_tag="PK288_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPM18910.1"
                     /translation="MKSIASALVALFILTGICAFRTGGNNKASDGNQNCRSGVAVIMT
                     GAAARIPQEAALLEELDNRGLFKDVVFISGVSSGALNSVMLNAVLSGKLSWEKYRNIL
                     FGLKNSDIFTQGQKKIPVNSAPERELFRRIVEDSLGYETIGDLPFTTAISFTHLQELG
                     VKKNVYRMCSRKINPESDTTLSLVDIMMASSAFPVAFPPVRIKNARTIPDVEYVDGGV
                     GEDHVPYHALLQFEEFRGKGVDTVYIISRKCDSIPEVSEELMNLGINDRGIFDRLGIS
                     LDEILKKGIIKRLKAYANEAPELVSRTFVWMPDFKADFLMFDFSNLQEQYEMTSQWAR
                     INEPVPLADFIRAEKKKER"
BASE COUNT         2924 a         2535 c         2596 g         3193 t
ORIGIN      
        1 agatcccggt tcgcggccct gaagacaaca atctcaggtc gttgcagatc tttgatagtt
       61 ttacagccag gcgttttatt gcggatgaat acattacaaa agatcctgta tcctgtgtgg
      121 cctctaccag gttggatgca aggaccaggt tgagacccgt gatcttcctc aggtgcctga
      181 tcactatttc cgaatagccg ggttcggcgg ttatgccggt tcctattgcc gttgcgccca
      241 tattgatctc aaggaacaat tttgcattct ggttcagcct ttcaacttct tccccgaggg
      301 tgaccgcata ggcttcaaac tcctgaccga gtgtcatggg gacagcatcc tgcagctgtg
      361 ttcttcccat tttaataaca cctgaaaact catgcccttt tttcctgaat gactcaatca
      421 gggtattcag aacagatacg attctcttgt ttgaattgaa cagtgcgatc tttacagccg
      481 tgggatatgc gtcgttggtt gactgggaca tgtttacatg attattcgga tgacagacgg
      541 aatagctgcc tttttcatgg ccgaggattt cctgggcccg gtttgcaatc acttcattgg
      601 cattcatatt tgttgaggta ccggcaccgc cctggatcat gtcaaccaca aactgattct
      661 ggtatttccc gttttgcagt tcatggcagg cttgcacaat agcatccttt atctttttat
      721 ccagcagccc cagctcgaaa ttcgcctttg cagctgcttc ctttaccatc gacaatgctt
      781 caattattga agggaaaaag ttcagggtta cccctgtgat attgaagttt tccgccgccc
      841 tgagtgtctg tatgccataa taagcctcat agggcacttc acgcactccg agaagatcct
      901 gttcagatct tgttcttcct gattcatact gggttgacag attcagcatc cggttgttga
      961 cattgcgcat tcgcctggaa ataacccggg tgatatttgc aaggatcttc agggtggttt
     1021 cagcattata atttaaaaac tccctgttga caacaagcag ggtggctttt tccagtatcc
     1081 ttgccgaagt tgaatgagtt gttttatgat cccatatccc ttcgccgagg aaatcaccct
     1141 tgccaaataa ggccagcctg acttcaacgc cataggaatc ctgtttgaaa agttcaacat
     1201 cgccctcgta aattatgaaa acatcttccc gcgggtcatt ctcatggaac agggtggccc
     1261 cggcattgta atgcttttct tttgcgttcc cggcaacttc ctgcagttca tcagcagtga
     1321 gattggtgaa aagctccacg tctttcagga actgaagaat tgtgttttta tccatgattt
     1381 atatctggca ttagtttcta ttaagttttg cgcgcagaac cctgatatct gttcctccga
     1441 atccaccgcc cgggatttga gaatgattca gtgaatcctt ctgaccggac aggcacaacc
     1501 ggtacgggtc tgcgaataat ttaccggtat gtgttttcca ttttgccctg gcagaacctg
     1561 gtgccttttg cataagaagg gccgcatttt ttgagccatc ctccttttat atttcattat
     1621 atctagtaaa cggcaagtat tcccttatca tatgttacgc agctattatt ttactccaac
     1681 gatttcattt ttatacgttt tgcctgtaag ggcagctgat ttgaccaggc cgggttcacg
     1741 cccctttacg tgatatggcg ggcaggactc ggtccatcca ggatttgatt caatcgtgaa
     1801 tctttaataa aaaccgctga atttaggatc ggagaacttt tttcaacccg atttgtttag
     1861 gatacagaag atgatcgtca acagcaaacg ggatcttcag gaaaataaca gatatccggt
     1921 gatcatgcca ggacccctgc acagccggcc ggtacgacag gacaggaacg aagagcgatc
     1981 aatccggaga aatcagccag actgaatctg aataaaaaac aagtataaaa tggaagcaca
     2041 cagtgtaaaa atcaaatcaa tcggacacat cacccgcgat gtgcttcaga tccagacgga
     2101 aagaccgccg gaactgaatt tcacacccgg acaggcaata gatattgcaa tagacaagaa
     2161 aaactggcag gaagaaaaga ggccatttac atttacaagc ctgccggatg atgattttct
     2221 tcagtttacg atcaagacct atccgtcacg gaacggagtg accaatcaac tgcttcacct
     2281 gaaaaggaat gacagacttc tcctgtacga cgtttttggt gcaattgaat atcgcaagga
     2341 aggatatttc attgcgggag gagcgggtat aactcctttt atctccatct ttaaatacct
     2401 ggggtcagtc aatgccatag gaaataataa gctgatctat gccaacaaaa caaatgacga
     2461 tattatcctg aaatatgagt ttgagtatct tctgggaaag aatttcatta atatcctgtc
     2521 agatgaaaaa gtggaaggtt atgcatatgg ttatataacc agggacttcc tggaaaaaca
     2581 gatagctgat ttcggaaaga acatttatct gtgcgggcca ccgcccatga tggatgcagt
     2641 tgaaaagata ctgtcggatc tgaatgtaga taaaagcctg atagtcaagg aagctgactg
     2701 aaatctgttt cagcagtcag ggccgaactg gaatttagat gtaccgcaga ttaaggcgtt
     2761 acaaaatatt atgataattg aagtttattg aataaattac aggaaaaatt aaacaaaatt
     2821 atggcaatca gtaaagtttg gatcgaggat ggatgtacat cctgcgggct gtgtgaagat
     2881 atctgccccg aagtcttcaa ggttgaagat attgcaacgg ttaaagaagg tgttaacttc
     2941 aatgattttg aggagggaat caaggatgca gccgacagct gtccggtgga agtgataaaa
     3001 tatgagtaag ggcagcatat attaaacgca tttattaacc tgattatcaa ataacaataa
     3061 aacaccacag ttatggcaac atcaaaagca caagcgcact ggaaaggaaa gattaaagag
     3121 ggtcggggca caatgaaatt caccggatac gaaggcacct acacatttgc ctcgcgtttc
     3181 gagaatgccg gagggactaa tcctgaggaa ctggtgggcg cggcacatgc cggatgctat
     3241 tccatgtatc tttcccttct gcttaccgaa gaagggcttg accctgaaag cattgagaca
     3301 acggccgaag taaccattga taaggatgat tccggtccgc atattaagga tattaagctt
     3361 cactgcaagg ttaaatgcag cgggctgtct gagggcaggc tgaaggaact ggcggctgtc
     3421 accaaggaaa aatgcccggt ttctcggttg tatgccggtg gaactgcaaa aataagtgcc
     3481 gaggcactgt taattaaata acagtagctg agacaggccg gataagctga ttggtttttt
     3541 gatgatttaa cggtgatccg gaaacggggg cggggatcgg ggatcgcgat tcagggatat
     3601 aatctgcccc tggtccatgg agtattggta ttttccctgc gatagaagat cctgtcatgg
     3661 agacggtggg tcatgccctg ccagaactca atgtataaag gagacaggca ataaccgccc
     3721 cagttgtcgg gtcgtttaat ggtctgatcc ttcagcatgt tttccagttc cctgacccgt
     3781 tcttccagta tcctcctgtt ttccagcggt ctgctttgcc gggaagccag tgcactgata
     3841 ttactctcac gcggacgact tgaaaaatat gaatccgatt ccccgggagg taatttgaaa
     3901 actgtacctt caattcggac ctgccggtac gatttgctcc agaaaaaggt catggatgca
     3961 ttattgttga tcttcagatc tgatcctttt ctgctgtcgt agttggtata gaaaacaaaa
     4021 cctctctgat caaatcccct gagcagcacg attcttcccg acggccttcc gtcgggtcct
     4081 gttgttgcga gatggaccgc attggccatt tcgggatcct ccctcattgc ctcatccatc
     4141 catttttcaa actgatcgat tggatctgct gagagttctt cctcattcag gccatcagag
     4201 ataaattcgc ttcgggagct gacaattttc tttcgtaccg atttcatccc cggtataata
     4261 aaatttgtta aagctaaatt ataacaatct ctccatgaaa atgttttgcc tgtctgcagc
     4321 agtcagctcc ggataaaagt gccgggcagg aatcaaacgt ttcgcagatc agggcaaaca
     4381 tgcgttgcta tgaattggcg gtaatctttg ccgcttcgat cgcattgact cctgaccggc
     4441 cgaatggccg gggcataggt tgcgctgtgc cgtttgcatc gattgttctg caccggaggt
     4501 cgtattttcc gggtatatca atttttgccg atgcggccca gtgaactatc gtgttgggaa
     4561 gcggccagga aacaagcttc cccgttttac tgtcaatttg catgaggttg ccaggcagtt
     4621 ttccgccggg caaatcgctg ccccagttcg cgggcggcgg taagatcatt gcatccctcc
     4681 agtctgccag tgccaggtag ggatcattgg cgtcaggggg ctcatcggca ggcattatgc
     4741 aatactgaac cttactcagt cccagcaggc cgacctgggc cattcccgta aaggcaaaca
     4801 tctgtttcgt cctgacttca gccggcacct taatgaactt agcgcatgtt tttacaggac
     4861 tttcgacatc attgttccag tcggcgtacg tgtcgttggc ctgatgattg tttgtaagca
     4921 ctattttctg aagccatttg attgagcggt tgccgtagaa tccgggaacg aataacctga
     4981 ccggtccgcc gttggcatga gaaaagtatt gtccgttaag tttataggcg aggatgacag
     5041 gcagttctcc ggggatctcc tcgagcaccc ggttgatggg cagggaactt tgaaaaatct
     5101 gtttgggatc gttgttgtga tagcccgaga agcggatccg tctgatgttc cgcccgggtt
     5161 ttgtctgcca tagtacctca cgcaggggta ctccctccca cagtcccata ccgtagggtt
     5221 ttggtgcatt tgtgcagctg aggacatgaa ggtagcttac ggcatgcttt tcagccatct
     5281 tcatcaggcc ggaccaggtg agggcgttgt tgttttcaat ggtaaaaggg ttccccagtt
     5341 cgctgtcgct ttcgggatcg gagatgattt caagtctcca ggtttcctga ctaagtccga
     5401 ttgacggcag aagttcttcg ggcagctcgg gaagcaccgg attgcctctg cgctggacga
     5461 tgaatttttt tgccggggta agatattcaa gctggctgat ggcttctgcg aggatcggat
     5521 cctggcggag ggcgtcgggc cgcagtccgg cattaaaggc cggcggacag atctcatttt
     5581 cagggatttt caggaatgat attcccgccg acatcacccc tattgttttt aatgcttccc
     5641 gccttgaaat gccttcattg tctttcagcg gtttgctgat ccttttcctg gagttatcct
     5701 tgagttctgc catggaatat tgtgtttttc cggtacctgt gtactttaaa tttcaaattt
     5761 aacaaaattc ctgtccggct tctgattatc acaattttta acactttgct gaattcagac
     5821 aatctgattt gcggaattgg acggttcaga tgtataagcc ttaacagatc ccgcatcact
     5881 gccgcctgct gttatcagtg accgggtgaa agccggagaa aaaggaagtc cgcccctttc
     5941 ggttctgtgg tattgtggag gcgatggaat aagccggttc cgggttgttt catttcttca
     6001 gaacgtcaaa tcctttcccg tagacaccca ttacgctggc aacggtgatg aaggccttgt
     6061 cgtcaatgct cttgatgatc cggtaaacat cactggtctc gttttttctg accagggtta
     6121 ttattatctt ctggctgtcc ttggtgtacc atcctgttcc gtcgataacc gtcactcccc
     6181 tgcgcatccg ggacgtgatc tggtcggcta tcgcttcata atgcttcgaa aaaatgaaaa
     6241 tctgaactga ctgcctgtag cccgaaagta ttgcatcgag ggtataggcc tggatagcca
     6301 tggtcacgaa accgtaaacc attgtttcca ccctctgagc cgagtcgagg tgtgtaagga
     6361 ccacatatga ggagctgatt atgaacacat cgcacaggag aattatcctg ccgggactgt
     6421 aattccggta ttttattatt atcagtgcaa ttatatcagt tccccctgtg ctgccaccct
     6481 ggctgaagac aatggccaga ccggttccgc aaaggatgcc tccgattatg gctgacataa
     6541 acttctcctg aaccaccgcc gtcgggataa gtgactgaaa cactgtgagc atgatggcag
     6601 acaacacaat tccgtagatg gttttcacgc cgaagcctgt tcccagaatc ctgagtccgg
     6661 caattacaag tattacattt atggcaagat aggtgtatcc catgggtatt cccgtggcat
     6721 agaatactat ggcgccgata cccgaaacac cgctgcctgc aattctgtga ggcaggagga
     6781 aggcggtcca tgctacggac ataagcatca ggccaaatgt gatgactgca taggtcctga
     6841 cattgtcaat gattgtacga aacaaaacag gcggctttaa aatcacatgc aaaggtatat
     6901 tgcatgccct gtacttccaa aaagttaatg tttctttatt tcagccactg caattttccg
     6961 tatatgccag gcaggttttt aagtaatttt ggcagagtgc ggatcaatcc gcaataaaaa
     7021 aactgcagcg ccagttaacc agcgctgcag cctgtaatgt attgcatatc gttttattcc
     7081 ccccctccga aatagaagtt aagggtgatt ttggcaattg tgggtgcaat tcttgcgcag
     7141 aatttgtctg tttcaactgc ggtttctccc cagtattcat attcatgcct gtcagtatag
     7201 ttacccccca gaaaacgtat tgcatattcg ccgcctatgc tgaaattatt gctgaaaaaa
     7261 tactccgctc caaaaccaag ttctgcaccg aacagtttga tttttttcag atcttctgag
     7321 aaatcttcat ttacatcacc gtcttctttc atttttccac tgatcatagg tttactgccg
     7381 ctgagattga aataggcctt gaggccgcct ttttcaacag caaataattt cactccgact
     7441 gacggcataa gtaaatttaa atttactgat ctgcttgaca catcagtaac ccattcggaa
     7501 ccattataat aatggtcctt atattcattc ttaaatccgc cgttggccgc ctgtaatccg
     7561 atccacggga caacctttcc ggccttatag ccaaaatagg ccgaattcgt tgcaattcct
     7621 ggagccacac caaaggaaaa ctggctgtgg gcgttaagtg aaatggcaaa aaccattatc
     7681 gcaataagga tgctcttttt catttcaatt gattttaatt tgttaataat gattgattat
     7741 ttatttgtat tcatgtatcc gataactatc atctgatccc gggctgttca taaagccggc
     7801 cacggccagt ttttatccgg atgaggattt ggatgtgtga aataattcac agcatcagaa
     7861 accagcgaat tgttaactga atgcagattt gtcttttttt cagatgtttc aacatttgcc
     7921 gacacaattt ttaaaacagt atgttttgaa ttctaacgga cggtttcgtg gaaaattgcg
     7981 gaagcctgcg agttctgcga agtttgcaac tacacatggg gcttttttgt tggaactagt
     8041 actgttattc ccaaggggat atcttattga aaagtccctt tccgggtggt atggcagggt
     8101 ggatatgttt attttgttta actaatttta aattaattca acgaaagttg atgctgatcc
     8161 attcgcggtg tgatcgaagt gcgattccga caggcggaac cgggattgct ttatttttgt
     8221 aattttgttt cagtgattca gtattgatgg atttgatttt ttgcagtctc agcagtggaa
     8281 gcagtggaaa ctgttattat ctgggcaaca ggggtcatgg gattttaatc gatgcaggca
     8341 tttctgcaac ctccatccgg aattatctaa ggacaataaa tgtttccatg cgttcgatag
     8401 atggtgtggt tgttacccat aatcattcgg accatatcag ggggctggag gtgtttacaa
     8461 gaaagaacag tatcccggca tacgcaacaa tgaaggtccg ggaaagcatt ttagctgcag
     8521 agaaaaaaat ttcacccggg tatattaagg aaatccccat gaggcaggca ttcagcctgg
     8581 caggttttga aatcgaagta tttccggtat gtcatgatgc accggaaacc attggattgc
     8641 atatttccgc cggaacaaaa aaaattacaa tagccaccga cctcggacat atctgtcaga
     8701 catctgcacc ttatattaaa gctgcaaatc ttctcgtgat agaatcaaac tatgatgagc
     8761 agatgctatt aaacggaaaa tacccgcaat tcctgaaagc aaggatcaaa tctgaccatg
     8821 gtcacctggg aaatcatcag gcagcttcct tccttgctga aatggcaggc gataatcttg
     8881 aaaacatttg tcttgcccat ctgagcctga acaacaatac tccggagctg gcactcaata
     8941 cgctgtataa ggccttttcg gaaaagggga ttgatttaaa tggtcacccc cgtatattgg
     9001 tactggaacg taacaggcct tcggaaatga taaaactgtg aaaaaaaccg gggcgtcaat
     9061 ccggtactca agaaaaaatc ttttgtaagg attcctcctg taccattggt tgcttctatc
     9121 tggctatttc acagatatga ccttaatgat atttgaggtg ataaccgaat tcggaacaaa
     9181 gatcctgcta ttttcttcgt tctcgataac tgtgtcgtgc catgttatcc tgacaacctt
     9241 acccctgaaa tcctgtacct caatggtatc gtggatcctg aagggtctgt tcaggatcag
     9301 gaacataccg ctcatgagat tgctgagtat ctgctgggct gaaaaaccca tgatcagggt
     9361 tgttaccccg gctcccgcaa acagggagtg ggcaaaactc ttcgttattg gcaatgagag
     9421 aaatgcccag ccaaccccaa aaagatatat tgtaagacga atgatgtttt taataaaaac
     9481 aatactcgtg atatcattac cagcggcttt gctcctgctg acaagcttat tcatcagtcc
     9541 tttgtcaata atgactgaaa caagtattgt tacgataatg attgatccga aaaggataag
     9601 agattcggta tcatgaaaca gcgtatcaag tcttttcatt ttttcggccg gtatagtgaa
     9661 atgaatagtt actggtggta aatttaatga tttgaaattc agcctgtgat tatctttcct
     9721 ttttcttctc agcccggata aaatcggcga gaggtaccgg ctcattgatc ctggcccatt
     9781 gggaagtcat ttcatactgc tcctgcagat tgctgaaatc aaacatgaga aaatccgctt
     9841 taaagtcagg catccagaca aaagtacggg aaaccagttc aggtgcttca ttggcatagg
     9901 ctttaagtct tttgatgatt ccttttttaa ggatctcgtc aagtgaaata cccagcctgt
     9961 caaatattcc cctgtcatta atgcccaggt tcataagttc ttcgcttacc tcgggtatac
    10021 tgtcacattt ccggcttata atgtaaaccg tatccacgcc ctttcccctg aattcttcga
    10081 attgcaggag tgcgtggtag ggtacatgat cttcaccaac tcccccgtca acgtattcaa
    10141 cgtcgggaat agtacgggca ttttttatcc tgacgggagg gaaggccacc gggaatgcgg
    10201 atgatgccat cattatatcg acgaggctca gggttgtgtc agactcagga ttgatcttgc
    10261 ggctgcacat cctgtaaacg tttttcttta cacccagttc ctgaaggtga gtgaatgata
    10321 tcgcggttgt gaacggcaga tcgcctatcg tttcgtatcc gagactgtct tccactatcc
    10381 tcctgaacag ttctctttcg ggtgcggagt taaccgggat tttcttctgg ccctgggtga
    10441 aaatatcact gtttttcagg ccgaaaagta tgttccggta cttctcccac gataatttac
    10501 cgcttagaac agcgtttagc atcactgaat taagcgcccc ggagcttaca ccggagatga
    10561 atacgacatc tttgaacagg cctctgttgt caagttcctc gagcaaggca gcctcctgcg
    10621 ggattctggc ggcggctcct gtcattatta ctgcaacacc gctgcggcag ttctgatttc
    10681 catctgatgc tttattattt ccgcctgttc tgaaagcgca tattcctgtc agaatgaata
    10741 atgcaacaag tgcggaagca atgcttttca tggcccgggt taactggaat gacatacagt
    10801 gcatcgaagc aataaaatta caataaagtt acgggaaaag agataatttt cagtgcgggg
    10861 aaccatggga tagtctatgt taaacagata aacttttatg cagcatatgg tcctatcagc
    10921 tgaacccgtc ggacaggtac cgtgagaacc gctccccggt acctcagcca gggagtcagg
    10981 tgccggagca ttgagacaac tctgcctgac tgaacatcat tttctgtatc atgaattcac
    11041 cggcattgcc tgtctgtacc gggaccggtc ccggccatat taaccatagc atggttttac
    11101 cgttgcttga ttttttaata caacacggaa ccggttacca caggatgagg atccggatcg
    11161 ctggtaaggc agagttatac gatttgcggc actcatatgt cagcattcac attcctttcc
    11221 ccttgcttct tcgaaagctt ccctgcct
//
LOCUS       DAOOYG010000084        11127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6260_length_11127_cov_7.155347, whole genome shotgun sequence.
ACCESSION   DAOOYG010000084 DAOOYG010000000
VERSION     DAOOYG010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6260_length_11127_cov_7.155347"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1381
                     /locus_tag="PK288_08025"
     CDS             347..1381
                     /locus_tag="PK288_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM18911.1"
                     /translation="MVSRRGTLKLLCYAGLAGFIQDFNNNMNENRKLTGSGFEPVYLK
                     LHRNGELKRRGEELWSMMESCRLCPRMCGANRLRGEKGFCGSGSQLEISSFHPHFGEE
                     KPLVGNGGSGTVFMSNCSLRCVFCINWEISQGGEGFTRSIDQLAGMMLSLQRTGCKNI
                     NIVTPTHYSPHIMLALDRAAAGGLRLPLVYNTCGWERIEILRMLDGVVDIYLPDFKYS
                     DGKMASKYSSGAESYPDIVKTALLEMHRQAGVAIPEPDGLMYRDLMIRHLVMPNNVSG
                     TREVIDWISENLPKNTYLNLMSQYSPHYKASVYPEISRRITRAEYADAVNHAKRSGLS
                     NLEIQGYHWL"
     gene            1727..3898
                     /locus_tag="PK288_08030"
     CDS             1727..3898
                     /locus_tag="PK288_08030"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HPM18912.1"
                     /translation="MTADHKVVSARFRIHLLSNPGFLFFIMLGFFCLAPASPQGNHIT
                     DSLSVLLKQSKRQAALLNKIAAEYNHISYSGALGIATEALEAARKEGNEHEAAYAWYN
                     MACNYMSRGAYDSSGIMLERSLKLAEKLNDELAILRVGNANASISFLKGRLDESFRGF
                     ESTLERARGAGFTEIIISAVANIGRIHWLQGRNESALRCYDEALFIADSVKNKFMSGM
                     IRLFRGILYQAMGFYELAAGSISVSAAVFEHMNYLDKLPYAYNYLGSVYMDLGENDNA
                     MTFFRKALMLFERNNDIWGKALTARFMGSIYSEKGRPDSAGFFIRESLALAARLNDLN
                     GMLFSRRFLAELMLKTGKPDSARVLLLGNLEDALKTDNRQEMVNNLYDLGRVYTHEGR
                     YSSALNYFHRAGALADSLGYFHEGMLVSKHLSGLYEKLGDYAKALYWNKQYKSLTDTI
                     FSTEKRRSIDELRLKYETEKKNSEISTLMMEKKVQDENLRTQRILGYALFAILGLVLV
                     ATVILWRNYRQKKKSEREKEILLKEIHHRVKNNLQTISSLLSLQSYNIADPGVKIAVK
                     ESQERVKSMALIHQMLYQQEKLSEIDFGLYLQRLAESVSSGYGDKAATISYHVDCNNV
                     ALDIDTAIPLGLIANELIVNAYKYAFIGKTSGDIFISLSRNSNNKYLFRIKDNGIGIP
                     GHISPDGAGTLGLKLVSLLVRQIKGVMVFLCMEGTEITITF"
     gene            3981..4733
                     /locus_tag="PK288_08035"
     CDS             3981..4733
                     /locus_tag="PK288_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM18913.1"
                     /translation="MEASGDIRILIVEDELIIAEDIRTKLVSLGYIVTGMAVSADEAE
                     QLLKNIKPDLVILDIMLSGERDGIDLARVIRDQYKVPFIFLTSYADKETVERARRVMP
                     DGYLVKPFTDKDLFAAIEVAVFRKSRVNGHVDDGLRETPSEILNDCIFIKKDYLLVKI
                     KFNDLLWIKSDGNYLELFCSGGKKHLIRSTLKDFINKLPGSVFLQIHKSYVVNINHIE
                     AIEYSHMIVGNYNIPLGRLFIDKIKDTLHIDF"
     gene            5080..6405
                     /locus_tag="PK288_08040"
     CDS             5080..6405
                     /locus_tag="PK288_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM18914.1"
                     /translation="MKKAIILMILFAPLICINSQIKVDVKGKINREADRRANRKADEA
                     VTKVFDKAEEGVVSVFRKKEQSRKESGSKEAATGNAAKKEAGGEVNREVSQAAGQVNE
                     QASQVAPDKQKPELVWAKYDFVPGDRILFEDNHENEQNGEFPARWDLVYGGGIENAVF
                     DNHKVIYFRSGDAYIVPYLKDPKSDYLPDVFTLEFDAWFEGREYCYYHIWLCDRKNQK
                     NLPDACNLITITANGVDYKGVGGSLYPGARYSDHHDDSFWRHVALSFNIRALKVYLVD
                     ARLVNIPNLECNPTGITLGVPGFNSAGTKGINRFIRNIRIAEGGVKLYDRLLQDGKII
                     ASGIRFDVNKATLKPESMGVINTICQLMNEHPELRFSVEGHTDSDGEEAFNQRLSEQR
                     AATVVSTLMQLGIDGSRLTSKGWGESKPVSDNTTPEGKANNRRVEFVKI"
     gene            6422..8311
                     /locus_tag="PK288_08045"
     CDS             6422..8311
                     /locus_tag="PK288_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18915.1"
                     /translation="MKNTGYRKAYAFILLFRVLFFFSGTISAQPAGCSDCSRTAVLGS
                     TTLTDPMHLVNADAFRSSVFSRIAELVTTPCFHLGGADADSYTGSLRDAGNLPGYGTE
                     TKTPEYIFTSELVSGLNETGADGRPVRSKWTISLWFEGKQREMVHSWQTLGTQEKRPA
                     SPTERNTGTTFSGHGNMMSEQFRGSHGITEIIERFEKRPVECTVDPEYEEVGAGAMME
                     IRVKEFKDRYGQTSREFNRIVVHARNGEITNGEECDIGPDYRVFMIGDGTVRIQYQAP
                     EETNENSEIITIYNACEILPKYKVPYSKTTIATRIAEKRMEITSNDAELTLTATRRKS
                     RMTSSQGSYQVREGKDCRKSFSSHKEFLEESDVRVKISLKRSELSNVTQNPLIMTNQT
                     LLAFTPVSAEISAFVYRLHESSEGREEVTGSDCRNNGSEGSSLRTRSVTGTPSFVFLP
                     ATYIPASVQVIFDSKTGKALKFAFPDMDIKYSYHETREGWSRWWPTDSPPSSWAENEE
                     TETSLRVGPVEDPVPDPYSFELSYNPLLDTLAAMKKRARGEMAEQLMSMVMAQAEMAQ
                     AEMKMDARDVESSSTIRPDLLVTKGDGKSTFGGEGRKLTEKKLDNGTEREELTFSWLL
                     VLTKK"
     gene            8571..8894
                     /locus_tag="PK288_08050"
     CDS             8571..8894
                     /locus_tag="PK288_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18916.1"
                     /translation="MSDGGSTVKNRGICWSTGLTPAISDNKTNDGTGAGSFISSITGL
                     NPTTTYYVRAYATNSYGTGYDSAFSFTIKEDSFTDLRDGNVYKFAEIGDQVWMAENLR
                     FPFVV"
     gene            complement(9409..10254)
                     /locus_tag="PK288_08055"
     CDS             complement(9409..10254)
                     /locus_tag="PK288_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPM18917.1"
                     /translation="MKKYLFILMMTLASLNIVNAQTDAGGCKDHPLLSRLENFYISEC
                     EENYNELQLRTGSNKTETKEGNLFYIYYRYNSDAGVKPKSVLQIMKNYEAAITKNGGK
                     MLYKNSNSLDADLEATYYLSTKEKEYWIQLASFAGTDNAVEAFALKILEIEAMKQEVA
                     ASEMFEAIRKDGFVALYINFETGKSTIKPESAPIINQIVEMLQQNPDLKISIEGHTDN
                     TGTMASNQTLSESRAKSVMDALISKGIAAARLKSKGWGQTKPIADNSTGEGKEKNRRV
                     EIVKQ"
     gene            10695..11111
                     /locus_tag="PK288_08060"
     CDS             10695..11111
                     /locus_tag="PK288_08060"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPM18918.1"
                     /translation="MRKNKARGLFEDQFRQEKITNMKDPLEQLNKFIRWEDFRPVIDK
                     AFVVTDPSVGGRPAFDRVMMFKVLVLQRMYNLSDDNTEYQILDRHSFCKFLGIDSYAQ
                     VPDSKTIWHYREQLKKHESINEIFSLVSKRFILKPL"
BASE COUNT         3114 a         2362 c         2695 g         2956 t
ORIGIN      
        1 cacggagaaa cacggagcaa aaccctctgt gcccctctgt gccctctgtg gtgaaaaaaa
       61 cattcagcac cgagaacacg gagatccacg gagcagaatc ctctgtgccc ctctgtgccc
      121 tctgtggtga aaaaaacatt caggaccgag aacacagaga aacacggagc agaatcctct
      181 gtgcccctct gtgccctctg tggtgaagaa gaactcacca cggagaacac taacggatca
      241 ttcatgtata atccttcatc aggaacttac atggctgaat atttgctttt taccggtttt
      301 tttggtaatt tgtaagtttg tagtgtcagt taattttctg caagaaatgg tctcaagaag
      361 gggaactcta aagttgttat gctatgcagg actggctggt tttattcagg atttcaataa
      421 taatatgaac gaaaacagga aactaacagg ttcaggcttt gagcctgttt atttaaagtt
      481 gcacagaaat ggtgaactta aacggcgagg ggaggaatta tggtcaatga tggagagttg
      541 cagattgtgc ccgaggatgt gcggtgccaa caggctcagg ggtgagaagg gcttctgcgg
      601 atccggttcg cagctagaaa tatcttcatt tcaccctcat ttcggtgagg aaaaaccact
      661 ggttggcaat ggagggtcag gaacggtgtt catgtccaac tgtagtctca ggtgcgtttt
      721 ttgcataaac tgggagatca gccagggcgg tgaaggtttc acgcgaagta tcgaccagct
      781 tgctggtatg atgctttcgc ttcagagaac gggctgtaag aatatcaata ttgtaacccc
      841 aacccattac tcccctcata ttatgttagc cctcgacagg gcagctgcag gggggctcag
      901 gctcccgctt gtatataata cctgcggatg ggaaagaatt gaaattctca gaatgctcga
      961 cggggtggtg gatatctatc tcccggattt caagtactcc gacggaaaga tggcatcgaa
     1021 atactcatcg ggtgctgaat cgtatccgga tattgtaaaa acggcccttc ttgaaatgca
     1081 ccgccaggca ggggttgcca tacccgaacc ggacgggctg atgtaccgcg atcttatgat
     1141 ccgtcatctt gttatgccga acaatgtaag cggaaccagg gaagtgatcg actggatctc
     1201 ggaaaacctt ccgaaaaaca cttatttaaa ccttatgtct caatacagtc ctcattacaa
     1261 agcatccgtt tatcctgaga tatcgcgcag gatcaccagg gccgaatatg ccgatgccgt
     1321 taatcatgca aagagatcgg gcctttcaaa ccttgagata cagggatatc actggctgta
     1381 gatctttctg ccctgtccga taaatgagaa cagttttgct ttcaatggaa gacccgggat
     1441 tttctgccgg gttaaattat ttggtccata cggctcctga tctgccttga gcaggcaggt
     1501 tcctttttgt cagcattaag agggattgga tcattgttca ttccggggca gtattggcag
     1561 ctgacaatcg ccggatgcca cctgcggata taccggaaac catgctgttg aaaaagttgc
     1621 ataacccgcc cccgggcata ccggaaggca taaataatta ttcggatctg aaaaaatagt
     1681 attaaattgt agctgtaaac cggcttccaa aacactggat tcgctgatga ctgctgatca
     1741 caaagtggtt tctgcccgtt tcaggattca tctcctgagc aatccgggat ttttgttttt
     1801 cataatgctc ggtttctttt gtctcgctcc tgccagtcct cagggcaatc atattaccga
     1861 cagcctcagt gtcctgctga aacaatcgaa gcggcaagcc gcactgctga ataaaatcgc
     1921 tgccgaatac aaccacattt catattccgg ggccctcggg attgccactg aggcacttga
     1981 agctgccagg aaggagggta acgaacatga ggctgcctat gcctggtaca atatggcctg
     2041 taattacatg tcgcgaggag cctatgactc gtcggggata atgcttgaaa gatcacttaa
     2101 gctggctgag aaactgaacg atgagctggc gatccttcgg gtcggcaatg cgaatgccag
     2161 cataagtttc ctgaagggca gacttgatga atctttccgg gggtttgaga gcacccttga
     2221 gcgggcccgc ggggccggat tcactgagat catcatatcc gccgtcgcaa acattgggcg
     2281 tattcactgg ctgcagggca ggaatgaatc cgccctcaga tgttacgatg aggctctgtt
     2341 cattgctgac tcagtcaaaa acaagttcat gagtggcatg atccgtttgt tcaggggaat
     2401 tttgtaccag gcgatgggtt tttatgagct tgctgcaggg agtatcagcg tatctgccgc
     2461 ggtgtttgaa catatgaact acctcgataa gctgccgtat gcctataact atctcggctc
     2521 tgtttacatg gatctcgggg aaaatgacaa cgcgatgaca tttttcagga aggcacttat
     2581 gctttttgaa cgaaacaatg atatatgggg taaagcctta actgcacgct ttatgggatc
     2641 aatctacagt gagaaaggcc gccccgattc ggccggcttc ttcatcaggg aatcacttgc
     2701 cctggcagcc aggctgaatg atttgaacgg aatgcttttc tcacggcgat tcctggcaga
     2761 gctcatgttg aagaccggca aacccgacag cgcacgcgtg ctcctgctcg ggaacctgga
     2821 ggatgcattg aaaacagata acaggcagga gatggtcaat aatttgtatg acctgggcag
     2881 ggtgtacaca catgagggtc gttattcatc agcacttaac tatttccaca gagctggcgc
     2941 cctggccgat tcactgggtt atttccatga ggggatgctg gtcagcaagc acctctccgg
     3001 cctttatgag aaactgggcg actatgccaa agccctctat tggaataaac agtacaaatc
     3061 cctgacagat acgatctttt caactgagaa aaggcggtcg atcgacgaac tccggcttaa
     3121 atacgagact gagaagaaga acagtgagat cagcactctc atgatggaaa aaaaggtaca
     3181 ggacgaaaat ctgaggaccc agcgcatcct tgggtatgct cttttcgcta tcctcgggct
     3241 ggtgcttgtt gcgacagtca tcctctggag gaactaccgg cagaaaaaaa agtcggagag
     3301 ggaaaaggag atcctactaa aggagatcca tcacagggtc aagaataatc tgcagactat
     3361 ttcaagcctt ctgagcctgc agtcatacaa tatcgccgat ccgggagtaa agatcgccgt
     3421 taaggagagc caggagcgtg tcaaatcgat ggctttgata catcagatgc tgtaccagca
     3481 ggaaaagttg tcggaaatcg acttcgggct gtatcttcag agacttgcgg aatctgtttc
     3541 ttcaggatat ggcgataagg ctgccacgat atcgtatcat gttgattgca ataacgttgc
     3601 actggatatc gatacagcca ttcccctggg gctgattgcg aacgagctca ttgtgaacgc
     3661 gtacaaatac gcgtttattg gaaaaaccag cggcgacatt ttcatttcac tatcacggaa
     3721 cagcaataat aaatacctgt ttaggataaa ggacaatggt attgggattc ccgggcatat
     3781 atcacctgac ggagccggaa cactcggtct gaagcttgta tcactcctgg taaggcagat
     3841 aaaaggggtt atggtttttt tgtgtatgga gggaaccgag atcacaataa ctttttaatt
     3901 acggtagtta tagatttgtc agtaaatttg tattttgatt acaggcaaaa taaggccatt
     3961 aagatataaa tccgctggtt ttggaagcat cgggtgacat aaggatcctt atagtggagg
     4021 atgagctgat aatagctgaa gacatccgga ccaaacttgt cagccttgga tatattgtaa
     4081 ccgggatggc tgtttcggca gatgaggcgg aacaattact caaaaacatt aaacccgacc
     4141 ttgtcatact cgacatcatg ctgagtggtg aacgggacgg tattgatctc gcgcgcgtta
     4201 tcagggatca gtacaaagtg ccattcattt ttctgacttc atatgcagac aaggagaccg
     4261 ttgagagggc caggagagtg atgcctgacg gatatcttgt taagccattc acggataagg
     4321 atcttttcgc tgcaattgaa gtggctgttt tcaggaaaag ccgggtaaac ggacatgtgg
     4381 atgatggttt acgcgaaact ccaagcgaga tactaaatga ttgtattttc ataaaaaagg
     4441 attacctgct ggtcaaaata aagttcaatg accttctatg gatcaaatca gacgggaact
     4501 accttgaatt attctgcagc ggaggcaaaa aacacctgat aagatctaca cttaaagatt
     4561 ttattaataa actgcccggc tctgttttcc tccagatcca taaatcatat gtggttaata
     4621 taaatcatat tgaagcaatc gagtacagcc atatgatcgt tggaaattac aacatccctc
     4681 tcggacggct ttttattgat aagataaagg ataccctcca cattgatttc tgataccctc
     4741 cgggtagttt ctttcttttt tgtccctcgc tgcctgcttt tcatcacccg cggcggatgt
     4801 ttgtcacaaa atattgattg gtagtccgtt gatctttatt tttaggctgg gtcctgtgaa
     4861 ccgcgggagc tctgttataa ataccggggg tgactcagaa ccgacaattt catatgaagg
     4921 ctatcctcaa aaacagtaag acacagccag ggaaggtgca aacctgatca cgacattatt
     4981 gcagtatatc acttccctgt gcagttcatc acattctttt gtcttttatt cttctgattt
     5041 ataacttcat acaaaaagac aaacaagata atgcaggtta tgaaaaaagc aattattctc
     5101 atgatccttt ttgcgccctt aatctgtatt aactcgcaga taaaggttga cgtgaaaggc
     5161 aagataaaca gggaggctga ccgcagggct aaccgtaaag ctgatgaggc tgtgaccaaa
     5221 gtctttgata aggctgagga aggagtcgtc tctgtattca ggaaaaaaga gcagagcagg
     5281 aaagagagcg ggagcaagga ggcagcaacc ggaaacgccg cgaaaaaaga agctggcgga
     5341 gaggtaaaca gggaggtgtc ccaggctgcc ggacaggtaa atgagcaggc gtcacaggtt
     5401 gccccggata aacaaaaacc tgaactcgta tgggcgaaat atgattttgt cccgggcgac
     5461 aggattcttt ttgaagataa tcatgagaac gaacagaacg gcgagttccc tgcacggtgg
     5521 gatcttgttt acggcggggg catcgaaaat gccgtgtttg acaatcacaa ggtcatctat
     5581 ttcaggtcag gtgacgcata tatcgttcct tacctgaaag atccgaaatc ggattatctg
     5641 cctgatgtct tcacactcga attcgatgcc tggtttgaag ggcgggaata ttgctactat
     5701 catatatggc tctgtgaccg taaaaaccag aaaaacctgc cggatgcctg caacctgata
     5761 actataactg ccaatggagt tgattacaaa ggtgttggag ggtccttgta tccgggagcc
     5821 aggtacagtg atcaccacga cgacagtttc tggcggcacg tcgccctctc attcaacata
     5881 cgggcactga aagtttatct tgttgatgcc cgcctggtca atatacccaa tcttgagtgc
     5941 aatccgacgg gtatcaccct cggtgtgccc ggtttcaatt cggccggcac caaggggata
     6001 aaccgcttca tcaggaacat ccggatcgct gaaggagggg tgaagctgta tgacaggctt
     6061 ctgcaggacg ggaagatcat tgccagcggg ataaggtttg atgtgaacaa ggcaaccctg
     6121 aagccggaat caatgggagt gatcaataca atctgccagc tgatgaacga gcatcccgag
     6181 ctgagattca gtgtagaggg gcataccgac agcgatggtg aagaggcatt taaccagcga
     6241 ctctccgaac agagagcagc aaccgtggta agtaccctga tgcagcttgg gattgacggc
     6301 agccgcctga catcgaaggg gtggggtgag agcaaaccgg tcagcgacaa taccacgccg
     6361 gaggggaagg ccaataaccg gagagttgaa tttgtgaaga tctagcttaa acgacagatt
     6421 tatgaaaaat acaggttaca gaaaagctta cgcatttatc cttctgtttc gtgttttatt
     6481 ctttttctcc ggcaccatat ctgcacagcc agcaggatgt tccgattgca gccggacggc
     6541 cgttctgggc agtacaactc tgactgaccc catgcacctg gttaatgccg atgcattccg
     6601 ctcttcggtc ttttcccgga tagcggaatt ggtaaccacc ccgtgcttcc accttggcgg
     6661 agcagatgca gacagctaca ccgggagcct gagagatgcc ggtaacctgc cgggttatgg
     6721 gactgaaaca aagactccgg aatacatatt tacttctgaa cttgtttccg gattgaatga
     6781 gaccggtgcc gacggccggc ctgtaaggtc aaaatggacc atatctctct ggttcgaagg
     6841 gaagcagcgt gaaatggtcc atagctggca gaccctgggg acacaggaga aaagaccggc
     6901 atcacccacc gaacgaaata cgggaaccac tttttcaggg cacgggaata tgatgtcaga
     6961 gcagttccgc ggcagccatg gcattacgga aataatagaa cgatttgaga agagacctgt
     7021 tgaatgcacg gtggatcccg agtatgaaga ggtcggcgcc ggcgcgatga tggagatcag
     7081 ggtaaaagag ttcaaagaca ggtatggaca gacctcacgg gagtttaacc gcattgtcgt
     7141 gcatgcccgc aatggcgaga tcacgaacgg tgaggaatgc gacatcggcc ccgattacag
     7201 ggttttcatg atcggtgatg gtactgtcag gatacaatac caggcgcctg aagagaccaa
     7261 tgagaacagc gagataatca caatttacaa cgcatgtgag atacttccga agtacaaggt
     7321 accatacagc aaaaccacca ttgccaccag gattgccgaa aagcgcatgg agataaccag
     7381 caatgatgcg gaactgaccc tgacagccac cagacgcaaa agccgcatga cctcttcaca
     7441 gggaagttac caggtaagag aggggaagga ttgccgtaaa tcgttttctt cacataagga
     7501 gtttctggag gagtctgatg taagggttaa gatatccctg aaacggtctg aattgagcaa
     7561 tgtaacccag aacccactga taatgaccaa tcagacactg ctggccttta caccggtaag
     7621 tgctgaaata tctgcatttg tttacaggct ccacgagtct tctgaaggcc gggaagaagt
     7681 aaccggctct gactgccgta acaatgggtc tgaaggtagt tccctccgta caagaagcgt
     7741 aaccggtacg ccatcatttg tgtttttacc tgctacctac attcctgctt cagtccaggt
     7801 gatttttgac tccaaaacag gtaaagccct gaaattcgcc tttccggata tggatattaa
     7861 atacagctat catgagacac gggagggatg gtcgagatgg tggccgacag attctccccc
     7921 tagctcctgg gcagaaaatg aagaaacgga aacttcactc cgtgttggcc ctgtggaaga
     7981 tcctgttccc gatccttact ctttcgaatt aagttacaat cccctgctcg acacacttgc
     8041 cgcaatgaag aagagagcca ggggggaaat ggcagaacag cttatgtcaa tggttatggc
     8101 tcaggcagag atggctcagg cagagatgaa aatggatgcc cgggatgtag aatcatcttc
     8161 aacaatccgg cctgatctcc tggttacaaa aggtgacggg aaaagcacct tcggcggaga
     8221 aggcaggaaa ctgactgaaa agaaactgga taatggcact gaacgggagg agctcacctt
     8281 ttcgtggctg cttgtcctga caaagaaatg aactaaatct ataaccatta tacgggtcaa
     8341 ttctgaatct aaaaaagtaa tcgggcatta gagaattaga acaagtttta accattgatt
     8401 taaagagaga tgaataagca actattttta aattatccag tgcaaataat attagcatta
     8461 ttgtctttta tgtttagctg tggagaagac aaagacaata tacctgtttt ttaacaacta
     8521 tggacgttac caacataact tcgcattcag ctgtaactgg tggtaatatt atgtctgatg
     8581 gcggatcaac tgttaaaaac cgtgggatat gctggagcac agggcttaca ccggcaatat
     8641 cagataacaa aaccaatgat ggaacaggag caggcagctt tatcagcagt attaccggct
     8701 taaatcctac aactacttat tatgtacgtg cttacgcaac taatagttac gggacaggat
     8761 atgacagtgc tttctctttt acaataaaag aagatagttt tactgacctg cgcgacggca
     8821 atgtttacaa atttgcagaa ataggggatc aggtatggat ggcagaaaac ctgaggtttc
     8881 cgttcgttgt ataagagatt aaagaacctg tgaaaatttt gtttaaactg gtaactgcaa
     8941 ggttaaaaaa agaatctttt ttcaccacgg agttcacaga gggacacaga ggattcttct
     9001 ccgtgtttct ccgtgctctc cgtggtaaga ttttttcacc acggagttca cagaggagca
     9061 cagaggattc tgctccgtgt atctccgtgt tctctgtggt aagatactta atcagcttga
     9121 atatccagcc aggttattaa tttccaggga caggagaact ttcaattcat taatattaac
     9181 gtgagttcga tataaagtta aaaagataaa gccaatgatt catcaataat ctccagatta
     9241 agagatggtt cctttggcag atagtaataa gcgattaatc cagataacag attgataata
     9301 aacccttcct ggcttctata atgggtgtgt tatatcttgt ccgggattaa agggcatact
     9361 gcccggcagg cgctgaggcg cctgctgatg aatcatattg tcggaatttt attgttttac
     9421 aatttcaacc ctccggtttt tctctttccc ttcaccggta ctattatcag cgattggctt
     9481 tgtctgcccc cagccttttg attttaagcg tgcagctgca ataccttttg aaatcagggc
     9541 atccatcacc gatttcgctc tcgattctga aagggtttga tttgatgcca ttgtgccggt
     9601 attgtcggtg tgcccttcga tgctgatttt cagatcagga ttttgctgca gcatttccac
     9661 gatctggttg atgataggtg ccgattcggg ttttatagtg gattttcctg tttcaaaatt
     9721 gatatacaaa gctacaaaac catcctttct gatagcttcg aacatttcac ttgccgccac
     9781 ttcctgtttc atcgcttcta tttccagtat cttcagggcg aatgcttcaa cagcattgtc
     9841 ggttcctgca aatgaagcaa gctggatcca atattccttt tctttggttg acaggtagta
     9901 tgtggcttcc aggtctgcat cgagcgaatt tgagttctta tacagcattt tacccccgtt
     9961 ttttgtaatt gctgcctcgt aattcttcat tatctgcagc acactttttg gtttcacgcc
    10021 tgcatcggag ttgtatctgt aataaatata gaacaggttg ccttctttgg tctctgtttt
    10081 attgcttcct gtgcgaagct gaagttcgtt gtaattttcc tcacattcgg agatgtagaa
    10141 attttcgagc cgggataaca gcggatgatc cttgcagcca ccggcatcgg tttgggcgtt
    10201 gacaatattt aatgatgcca atgtcatcat aagaataaac agatactttt tcattgtttg
    10261 atgtttgttg gttaatgagt ttctgatcgg ttatctaaat ttctggtctt ttggctgata
    10321 tttaaaccat taaatcgcga atttgaaact gaggagctgt tgttatcccg cctttgatat
    10381 ggttttggtt ttcctgcagt tcatggtatg aaattacatt atgaaaacac cgggtataac
    10441 tggtttgatg attttgttat taacaggaca aaaaaaagtt gttattaaaa tcttcttatt
    10501 tttcccggag ggtcattgaa aaaccagtaa taattatcgc tgtctttttg tgctcaacgg
    10561 attgtaacag attctatctg gaattatctt atgggtacct ctaaatacca aattgttaat
    10621 aaaaagttta taactaattg tttatcaaat atatagatta atttgattgc gtatctttaa
    10681 taaaaaaaca aaagatgcgc aaaaataaag ctcgtggact ctttgaagac caatttcgcc
    10741 aggaaaagat caccaatatg aaagatcctt tggaacagtt aaacaagttt attcgatggg
    10801 aggatttccg tcctgtgatc gataaagcct ttgtggtaac tgatccttca gttggtggcc
    10861 ggcctgcctt tgatcgggtc atgatgttca aagtattggt tttacaaagg atgtacaatc
    10921 tttccgatga taataccgaa taccagatcc ttgaccgtca cagtttttgc aaattcctgg
    10981 gaatcgactc ttatgcacaa gtcccggatt ccaagaccat ttggcattat cgtgagcaat
    11041 tgaaaaagca tgagagcatt aatgagatat tttcactagt gtccaaaaga tttattttaa
    11101 aaccattgta aatggttaat atataat
//
LOCUS       DAOOYG010000085        11046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6328_length_11046_cov_6.257119, whole genome shotgun sequence.
ACCESSION   DAOOYG010000085 DAOOYG010000000
VERSION     DAOOYG010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6328_length_11046_cov_6.257119"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1133..2911
                     /gene="rpsA"
                     /locus_tag="PK288_08065"
     CDS             1133..2911
                     /gene="rpsA"
                     /locus_tag="PK288_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPM18919.1"
                     /translation="MSPEDFDWDSFEKDELKNSPRHKEYEAMYDKTLSTIAVDEVVQG
                     TVIQMTPREVVINIGYKSEGVVSLNEFRYNPNLKVGDVVEVYVESQEDKKGQLLLSHK
                     KARAINSWDRVNASLENDEIINGYIKCRTKGGMIVDVFGIEAFLPGSQIDVKPIRDYD
                     VYVGKTMEFKVVKINQEFKNVVVSHKALIEAELEQQKKEIIAKLEKGQVLEGTVKNIT
                     SYGVFIDLGGVDGLIHITDLSWGRVNHPEEIVQLDQKLNVVILDFDDDKKRIALGLKQ
                     LTPHPWDSVDPDLKVGDKVRGKVVVMADYGAFVEIAPGVEGLIHVSEMSWSQHLRSAQ
                     EFLKVGDEVEAVILTLDRAERKMSLGIKQLKPDPWQNIDEKYTIGSKHMAKVRNFTNF
                     GVFVEIEEGVDGLIHISDLSWTKKIKHPAEFTSIDSEIEVVVLEIDKANRRLSLGHKQ
                     LEENPWDVFEDLFTVDSIHEGTVIEVFDKGAVVALPYGVEGFVTPKNMVKEDGTLIKV
                     DEKLQFKVIEFNKSSKKIIISHSRVFEDEKKSADSTARKGEAETARKGSRKAKTNVEK
                     TTLGDITQLAALKEEMEEKEGKASKK"
     gene            2948..3286
                     /locus_tag="PK288_08070"
     CDS             2948..3286
                     /locus_tag="PK288_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPM18920.1"
                     /translation="MDFNIESRNNSTLIQIQTEKLDTHIAPALKSELVLISGKGEKNI
                     ILDLEKCQYCDSSGLSAILVANRLCKNAGGTFVLCGLSEAVDRLITISQLDTVLNITG
                     SVADAEKLIS"
     gene            3310..4239
                     /locus_tag="PK288_08075"
     CDS             3310..4239
                     /locus_tag="PK288_08075"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPM18921.1"
                     /translation="MKLTILGSSSALPTSERYPSAHVLNVHEHLFLIDCGEGTQMQLR
                     KCRIRFGKINNIFISHLHGDHVFGLYGLLSTFNLMGRTVPLSLFAPASYMAKLSSHLH
                     DFDINLNFQIDFHPLAGKEPVQIFENKHMTVTSFPLKHRIEAFGFLFREKPAERNIIR
                     ERIEELGISVAQIRAIKKGSDFVAADGRLIKNSDLTLDPEPPLSYAYCSDTAYFRGLA
                     GVVRNVDLLYHEATFATNMKELARQTGHSTAADAAKTARAASAGTLIIGHFSARYRDI
                     DPLVEEARTIFPSTIPALDGFTYDIAELARRIK"
     gene            4392..5987
                     /locus_tag="PK288_08080"
     CDS             4392..5987
                     /locus_tag="PK288_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPM18922.1"
                     /translation="MGRFLKLAFIVLAFILAGSKGYSQDPNEGTLKIGRTLGLIDAFY
                     VDTVNLGSLTEKAIIEMLRTLDPHSSYISAKDVKDMNEPLNGNFEGIGIQFNLLHDTI
                     IVIEPVAGGPSEKVGLQAGDKIITIDNERVTGINITTAGVRKRLMGMKGTKVEISVFR
                     KGEKNLLEFTIVRDKIPINSLDASYMLNNETGYIKLNKFAATTEKEFMDAITQLQKEK
                     IKNLIIDLRSNGGGYMLAATALAGKFFPDKKLLVYLSGRKTPRQDYNSSGMGILSSAK
                     VAILTDEGSASASEILAGALQDWDRGIIVGRRTFGKGLVQNGYYLPDGSMIRLTIARY
                     YTPTGRLIQSPYNEGYDKYIENFYKRYTTGELMSADSIHFPDSLKFKTLISQRTVYGG
                     GGIMPDIFVPADTSHYSDYYRNLVRRGVLNSFALEYSDGNRDKLSAEYKTFADFRKKF
                     SFTTDEIASFIRKAEEAGVKYNDEQFQKSKEEILIVLKGLLASNRWQTNAYYQIINEN
                     DKVIEKALEAVSDDSVYNRVLGR"
     gene            complement(5984..6418)
                     /locus_tag="PK288_08085"
     CDS             complement(5984..6418)
                     /locus_tag="PK288_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18923.1"
                     /translation="MMKKSVSLPVIILFSALLATFSCKKDEPVDKLLLGKWKVQSVTV
                     IYYENNVKIWEQTLYSIEPELIFQFSDGGTGTIYEDNEVKGAFTWQLKGDKLALDMGV
                     DDKPEWKISIDKNTFVWSFEEYETTNKVLQKLEYIYTATKIS"
     gene            6612..7745
                     /locus_tag="PK288_08090"
     CDS             6612..7745
                     /locus_tag="PK288_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPM18924.1"
                     /translation="MKYLAKTLFGLEEVLAKELEDAGASEVEPLNRAVSFRGDLKMLY
                     KANYCLRTALSILVPIAEFRIASARDLMKKSLLVEWARYMDAGQTFSVVPVVNSGLFS
                     HTGYPALVLKDAVADWFRMNKGRRPSVDTSDPHVVLNLHISGEAVTISLDSSGVPLYR
                     RGYRRGSGTAPLNEVLAAGIILLSGWDGSVPMLDPMCGSGTFPVEACLLACGIPPGKY
                     RKSFGFQRWNDFNPELFDEVRRECNALVPMKHAAISGSDISGEAVRLARLNVESAGLD
                     HLISIEKHDFRDIQSENREGIIFLNPPYGQRIKTHETETLYKMIGSTLKHNFPGFRAF
                     IISSDTEALKHVGLKPSVKRTLFNGSLKCSLMGYNLYAGSLKR"
     gene            7917..8489
                     /locus_tag="PK288_08095"
     CDS             7917..8489
                     /locus_tag="PK288_08095"
                     /inference="COORDINATES: protein motif:HMM:NF038262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HPM18925.1"
                     /translation="MSFDVEDYFSAVSSGDTILCFKGNVDSERINETLDTIEEKLIRD
                     NEPPRLRKRVYNVLVESLQNLYHHMEKAPPDFHDQKSERYGMLAINRTGNGYRIYTCN
                     FILNDNIPQLEEKIEQINRSTHKEISELYKFILNHQRISAKGGGGLGLVDIARKTGNK
                     LEYSFKKYNDKYSLFCMNALVREDNKNINT"
     gene            8495..8875
                     /locus_tag="PK288_08100"
     CDS             8495..8875
                     /locus_tag="PK288_08100"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HPM18926.1"
                     /translation="MKPIVIEGTPKTPSIKFDPSEGVFEISGRSIPENSVEFYRPLLE
                     WLDQYKEVPLEKTVVKIRLEYFNTSSSKCILDVFKKLEAIHKAKNEVEVNWYYEEDDE
                     DMLEAGEDYESIIRVPFKMMEIEA"
     gene            complement(8947..>11046)
                     /locus_tag="PK288_08105"
     CDS             complement(8947..>11046)
                     /locus_tag="PK288_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM18927.1"
                     /translation="GYVSQDDLLIEELTVFENLYYNARLCFGNLSETEIEERVDGVLK
                     SLGLHEIRNMKVGSPLNKKISGGQRKRLNISLELIREPAILFLDEPTSGLSSRDSENI
                     LDLLKELSRKGKLLFVVIHQPSSDIFKMFDKLIILDTGGYMIYNGNPVDSIEYFKKKI
                     GQADYSESECHSCGNVNPEQIFNIVETKVFTESGRPTSTRRISPGEWWKLYESEKKEE
                     HLEKGDSLPEINFRTPSWYKQFLVFAQRDILSKAADLQYVLITLLEAPVLAFFLAFLI
                     RYFDVSAKNPGYTLMDNSNLPVYLFMAVIVAIFMGLTVSAEEIIKDRKILRRESFLNL
                     SRSGYLASKMLVQFGISAVQSLSFVLIGNSITGIRGMTFEYWLVLFSCWASANVMGLL
                     ISDSFKTVVTIYILIPFLVIPQILLSGVMVKFEKLNPRISSPVDIPVYGEMITARWGY
                     EALAVKQFMDNRYERLFYEYDKDMSNARYKKDYWNSKLKGKLDILMLDLERGSRSDDF
                     NSNLLIVSNEIGKELKILPDISFANAPMLTPEKVTPEIISEAAGYVEAVRRYYVRFYN
                     EANSLKDLLITTMEEKDKSYFQKLRNDHSNVALEEFVTAKNETQKIIEYKNELVRKLD
                     PIFMDPGPAFIKAHFYSPEKRIFGRLTDTYVVNVAVIWFMTLVLYIILYFRLLHKLLV
                     SFESLSGKRNKTSDKET"
BASE COUNT         3202 a         2380 c         2566 g         2898 t
ORIGIN      
        1 cctgaaccgc cggcaagaat aattcctttc atagatgtca gttttacaat atggttaagt
       61 atacgagctt attattgtcc ggtcatgtac cggtttgatc cctgcctgaa aatctccggc
      121 cggccgttgt ttgttacttc cttcaaaaac tacccttcct gataccagtt cctgtaatcc
      181 tgattgttcc ggatgacaaa accctgtctc gccggagccg gggggaaagt caataatccc
      241 ggctgcctga aggctgcctt ccgcacggtc ggataaagac tttcctgttt tatccttatc
      301 ttgtttaaac ggtgtgaagc atgttcctat tgcttggttt accggatctg tattgaattt
      361 aaggggatca ttgtttaatg atatggcctt cataaagctg tttacagccc tgtcgctttt
      421 agggaaatat acagtttcag gagccgttat tttcataatg acttcgaaat caaaaaggat
      481 tatccgggca aatataatac aacgaactaa aaaatgaaac taacccggac agatgcctgc
      541 tgatgaatca aacagttgca gaagttttta ttttctgtat gttaacgctt gtattactgt
      601 taatttaata acacaccttt tccggagcgg aatttttggt ttgagcctat ctgagaccgg
      661 gctgtgccca ttgcggcttg actcagccga ttttttaact cagtgaaaac cttaaatata
      721 ttgtgtcaat tcataaaaaa tcatatcttt gcatcctgtt ttttatacaa aataatgtct
      781 aacttattaa ttattagatc ttttaattac aactatgact gacacaaaga agaaatcagg
      841 caataaggat aacattgaaa ggaatgacga tgaattcaga gccgaaaaaa agccaaaaat
      901 aaaggaagcc aacctgatgc aggagattga ggaggaattg ctggacacgg aactgcccgt
      961 ccgggattcg gcagatgctg aagttttcgc ggaaccttta aaagcagcag gggaacagga
     1021 aactgacgac ctgcatgttg aagattcaga cgacgaggtg ttttccgtgc ctggcattga
     1081 atctgttcag ggcgcagtac ccgacatcag gggaacaagc gaagcggcca ggatgtcacc
     1141 tgaggatttc gactgggata gtttcgaaaa ggatgaattg aaaaattcac ccagacacaa
     1201 ggagtacgag gcaatgtacg acaaaaccct gtcaaccatt gctgttgatg aagttgtaca
     1261 gggtaccgtt atccagatga cgccgcgcga ggttgtgata aatatcgggt acaaatcgga
     1321 aggagttgta agcctcaatg aattcaggta taatcccaac ctgaaggttg gcgatgttgt
     1381 ggaagtttat gttgaaagcc aggaagacaa aaagggccag ctgcttcttt ctcacaagaa
     1441 agcaagagcc ataaattcct gggacagggt aaatgcatct cttgaaaacg atgaaataat
     1501 aaacggttat atcaagtgca ggacgaaagg cggaatgatt gtggatgttt tcgggataga
     1561 agcttttctt ccgggatcac agatcgatgt aaaaccgatt cgcgattatg atgtttacgt
     1621 gggaaaaacc atggagttca aggttgtgaa gattaaccag gaattcaaga atgtggtagt
     1681 ttcacataaa gcgctgattg aagctgaact tgagcagcag aaaaaggaga taatagcaaa
     1741 actcgagaaa ggacaggtac tcgagggcac agtcaagaat ataacttcat acggtgtgtt
     1801 catcgatctg ggcggagtcg acggcctgat tcacataacc gacctttcat ggggaagggt
     1861 taaccatcct gaagaaattg ttcagcttga tcagaaactg aatgttgtca tcctcgattt
     1921 tgacgatgac aagaagcgta tagccctggg actcaagcag cttactcccc atccctggga
     1981 ttcggttgat cccgacctta aggttggcga caaggtcagg ggcaaggttg tggtaatggc
     2041 cgattacggg gctttcgtcg agattgctcc cggagtcgaa ggccttattc atgtttctga
     2101 aatgtcgtgg tcgcagcacc ttagaagtgc gcaggaattc cttaaggtgg gagatgaggt
     2161 ggaggcagta atactgaccc tcgacagggc agaaagaaaa atgtcactgg gcatcaagca
     2221 gcttaagccg gatccgtggc agaatatcga tgagaagtat acaataggct caaagcatat
     2281 ggccaaagtg aggaatttta ccaatttcgg agtttttgtt gagattgagg aaggcgttga
     2341 cggacttatt catatatcgg acctgtcgtg gacaaagaaa atcaaacatc ccgcagaatt
     2401 tacatcaatc gattccgaaa ttgaagttgt tgttcttgag attgacaagg caaaccgccg
     2461 cctgagcctt ggtcataaac agctcgagga aaatccatgg gatgtttttg aagacttgtt
     2521 cacagtcgat tccattcatg aaggcaccgt tatcgaagta ttcgacaaag gcgcggtggt
     2581 tgcccttcca tatggcgtcg aaggattcgt tacaccgaaa aacatggtga aggaagacgg
     2641 aacccttatc aaagttgatg aaaaacttca gttcaaggtg atcgagttca ataaatcctc
     2701 gaagaaaatc atcatttccc acagcagggt gttcgaagat gaaaagaaat cagccgacag
     2761 cacggccagg aaaggcgaag cagaaactgc caggaaagga tcgagaaaag caaaaacaaa
     2821 tgtcgaaaaa acaacccttg gcgacataac ccagcttgct gccctgaaag aggaaatgga
     2881 agaaaaagaa ggcaaagctt ccaaaaaata gaaataatag ttacatttag gctataataa
     2941 taaaactatg gattttaata ttgagagtcg gaataacagc acattaattc agatccagac
     3001 tgaaaagctg gatacccata ttgcccctgc gctaaaatcc gagcttgtac taatttctgg
     3061 taaaggagag aaaaacatta tactcgacct ggaaaaatgt cagtattgtg actcttccgg
     3121 tcttagcgcc attctggttg ccaacaggct gtgcaaaaat gccggcggga cctttgttct
     3181 ctgcggacta agcgaagctg tcgacagact gattaccatt tcccagcttg atactgtcct
     3241 taatattaca ggatcggtag ccgatgccga gaaattgatt tcctagcggg cctgtattgc
     3301 agattgccca tgaaactaac aatccttggc agcagctctg cattaccaac ttccgaaaga
     3361 tatccatctg ctcatgtact caatgtgcat gagcacctgt ttttaatcga ctgcggcgaa
     3421 ggaacacaga tgcagctgag aaaatgcagg atcaggttcg gtaaaataaa caatatcttt
     3481 atttcacatc tgcatggcga tcatgtgttc gggctttacg ggcttttgtc tactttcaat
     3541 ctgatgggca gaacggtgcc cttaagcctt tttgcccccg ccagctatat ggcaaagctc
     3601 agttcgcacc ttcacgattt cgatattaat cttaattttc agatcgattt tcatccgctg
     3661 gcaggaaagg agccggtaca gatttttgaa aacaaacata tgaccgttac ctcttttccc
     3721 ctgaaacaca ggatagaagc ttttgggttc ctcttcaggg aaaagcctgc ggaaagaaac
     3781 ataatcaggg aacgaattga agaactgggc atatcagtgg cacagataag ggctataaaa
     3841 aaaggcagcg attttgttgc agctgatggc agacttatca aaaacagcga tctgaccctg
     3901 gatccggagc ccccgctctc atacgcctat tgtagcgaca ctgcatattt ccggggattg
     3961 gccggggtgg tcaggaatgt cgatttgctt taccatgaag caactttcgc gacgaacatg
     4021 aaggaactgg ccaggcagac aggtcactca actgcagccg atgccgcgaa aaccgcaagg
     4081 gcagcatcgg ccggaacact cattatcggc catttttcag cacggtacag ggacatcgac
     4141 cctctggttg aagaagcaag gacaatattc ccatcaacaa ttccagccct cgacggtttt
     4201 acttacgata tagctgaact ggcccggaga atcaaatgaa agaataacta tctttacctg
     4261 cgttttcccg ggtaatggtg cagcaggaat gatatcccat gcattaatcc cggcatggag
     4321 tttgccggct gaatggaatc acacccttaa ttatcaggaa ccagtggaga actaatttaa
     4381 aacagaaaaa cgtgggaaga tttttaaaac ttgccttcat tgtcctcgct tttatactgg
     4441 ctggcagtaa aggctactcc caggatccca acgaagggac cctgaaaata ggccgtaccc
     4501 tggggctcat cgatgcattc tacgtcgata cggttaacct gggcagcctg actgaaaaag
     4561 ctataattga aatgctgcgg accctcgatc cgcattcctc ctatatctca gccaaggatg
     4621 taaaggacat gaacgaaccc ctcaacggta attttgaagg catcgggatc cagttcaacc
     4681 tccttcacga taccatcatt gtaattgaac ctgttgcagg aggaccttct gaaaaggtgg
     4741 gactgcaggc aggtgataaa ataattacga tcgataacga gagagttacg ggaataaaca
     4801 tcactacggc aggagtcagg aaacgactta tgggcatgaa aggcacaaag gttgaaataa
     4861 gtgttttcag gaaaggtgaa aaaaacctcc ttgaatttac gatagtccgt gacaaaatcc
     4921 cgataaacag ccttgatgcc tcctacatgc tgaataatga aacaggttac atcaagctga
     4981 acaaatttgc agcaaccacc gaaaaggaat tcatggatgc cattacccag ctgcagaagg
     5041 aaaaaatcaa aaaccttatc atagatcttc gctcgaacgg gggcggatat atgcttgcag
     5101 caacagcact cgccgggaaa ttctttcccg ataaaaaact tctcgtttat ctcagcggaa
     5161 ggaagacacc cagacaggac tataattcat ccgggatggg aatactgtca tcagcaaagg
     5221 tggccatact taccgatgag ggatccgcct cggcaagtga gatacttgca ggtgcccttc
     5281 aggactggga caggggaata atcgtagggc gccgtacatt cggaaaaggg ctggtgcaga
     5341 atggctatta tctccccgac ggctcaatga taagactaac aatcgcaaga tattacaccc
     5401 cgactggcag attgatacaa agcccatata atgagggata tgacaaatat attgagaact
     5461 tctacaaacg atatacaacg ggagaactga tgagtgccga cagcatccat tttcccgact
     5521 ccctgaagtt caaaacactt atcagccaaa ggaccgtgta tggaggcggg ggcattatgc
     5581 ccgatatctt cgtgccggcc gacacgtccc actattccga ctattacagg aacctcgtca
     5641 ggcgtggagt gttgaattcc tttgcactgg agtattctga cggaaaccgc gataaactgt
     5701 cggcagaata taaaacattc gcggatttca ggaaaaaatt cagtttcacg accgatgaga
     5761 ttgcatcgtt tattcggaaa gctgaggaag caggagtgaa atataatgat gaacagttcc
     5821 agaaatcaaa ggaagaaata ctcattgtgc ttaaaggcct tcttgccagc aacaggtggc
     5881 agacaaacgc ttattaccag ataataaacg aaaatgacaa agttattgaa aaggcactcg
     5941 aggcagtttc tgatgattca gtctacaaca gggtattggg acgttagctg atctttgtgg
     6001 ccgtgtagat atattcgagt ttctgcagta ccttgttagt ggtttcgtat tcttcaaagg
     6061 accagacaaa tgtgttctta tcgatgctta tcttccattc aggcttatca tcaacaccca
     6121 tgtcaagggc aagcttatct cctttcagct gccatgtgaa agcacctttt acttcattgt
     6181 cttcataaat tgtcccggta cctccgtctg aaaactgaaa tatcagctcc ggttcgatag
     6241 aatacagggt ctgctcccat atttttacat tgttttcata atatatcacg gtgactgatt
     6301 gcaccttcca cttgcccaga agaagtttgt cgaccggttc atctttcttg caggaaaatg
     6361 ttgccagcaa tgctgagaaa agaataatta caggcagtga gacagatttt ttcatcatta
     6421 tataaatttg attcgtatga tacgacttaa cctcacctga ccggagattt tttgtacttg
     6481 aaattcttgc cggtcatgca ggttaaataa gtatattttc aatttttggc aaggtacgtt
     6541 tttttatttt cactgttaac tttgaccggg atcatttgga ttaattttga agattattta
     6601 aaaatacagg gatgaaatat ctcgcaaaga cattattcgg gcttgaagaa gtacttgcga
     6661 aggagctcga agatgccgga gcgtcggagg ttgaacctct taaccgggct gtcagtttca
     6721 ggggcgatct gaaaatgctc tacaaggcaa attactgcct caggacagcc ctctccatac
     6781 ttgtacccat tgctgaattc cgcatagctt cagcccggga tctgatgaaa aagagcctgt
     6841 tggttgaatg ggccaggtac atggatgccg gccagacctt ttcagtggtt cctgttgtga
     6901 attccggatt attcagtcat accggctatc cggcacttgt gctgaaggat gcagttgccg
     6961 actggttcag gatgaacaaa ggccggcgcc cttccgttga cacgtcggat cctcatgttg
     7021 ttttgaattt gcatataagc ggcgaagctg ttacaatttc gcttgattcc tccggggttc
     7081 cgctgtaccg acggggttac cgcaggggat cgggaacagc gcccctgaat gaggtgcttg
     7141 ccgcaggtat tatcctgctt tcaggttggg acggctctgt gccgatgctc gaccctatgt
     7201 gcggttcagg tacattcccg gttgaagcct gccttctggc ctgcggaata cctcctggta
     7261 aataccggaa atctttcggt ttccagcgat ggaatgattt taacccggaa ttattcgatg
     7321 aggtgagaag ggaatgcaat gcgttggttc caatgaagca tgcagctatc agcggaagcg
     7381 acatctccgg ggaagcagtc agactggcaa gactcaacgt tgaatccgca gggctggatc
     7441 atctgatcag tattgaaaaa cacgatttca gggatatcca gtcagaaaac cgggaaggca
     7501 tcattttttt gaacccgccg tacggacaaa gaataaaaac ccacgaaacg gaaaccctgt
     7561 acaaaatgat cgggtcgaca ctgaaacata attttccggg cttcagggca ttcattatca
     7621 gttctgatac tgaggcactg aagcatgtgg gcctgaaacc atcagtgaaa cgaacactct
     7681 ttaacggatc gctgaaatgc agcctcatgg gatacaatct ttatgcagga tcccttaaaa
     7741 ggtaaattcc ttcgggaaac aggtgccgta acgttcagtt aaatcccggg ctccggcagc
     7801 ggtgctgata tctaaagcaa attgttaata ctattattga tgtgagttaa aaaagtatgg
     7861 aggtattttt attatatttg tttccggatt aatttgacta aaaaatagaa gcttagatga
     7921 gctttgatgt tgaagattac ttctcagctg tttccagcgg cgatactatt ctttgcttta
     7981 aaggtaatgt tgattcggaa aggataaacg aaaccctgga tacgattgag gaaaaactta
     8041 taagagacaa tgaacctccc cggctgagaa aaagagtcta caatgttctc gtggaaagcc
     8101 tgcagaacct ttatcatcac atggaaaaag ctcctcctga ttttcatgac cagaaatccg
     8161 aaagatatgg tatgctggca atcaacagga ccggtaacgg atacaggatt tatacctgta
     8221 acttcatttt gaatgacaat atcccgcagc ttgaggaaaa gattgagcaa atcaacaggt
     8281 cgacgcataa ggagatatca gaactgtaca agtttattct gaatcaccag aggatctcgg
     8341 caaaaggcgg gggaggactc ggccttgtgg atattgccag gaagaccggg aacaagcttg
     8401 aatattcgtt taagaaatac aatgataagt attcactgtt ttgcatgaat gcacttgtca
     8461 gggaagataa taagaacata aatacctaat aaagatgaaa ccaatagtta ttgaaggaac
     8521 accgaagact ccgtcaataa aatttgatcc ctccgaagga gtgtttgaaa tatcgggccg
     8581 gtcaatcccg gaaaactcgg ttgagtttta caggcctctc ctggagtggc tcgaccaata
     8641 caaggaagta cctcttgaaa agactgttgt taagatcagg ctggagtatt ttaatacaag
     8701 ttcttcaaaa tgcatacttg atgttttcaa gaaactggaa gctatccaca aggcaaaaaa
     8761 tgaggttgag gtaaactggt attatgagga ggatgatgag gatatgcttg aagcaggcga
     8821 ggattatgaa tcaatcatcc gcgttccgtt taaaatgatg gagatagagg cctgagcatt
     8881 ataattattt tttgaaattt ccgatgacat cattcaagag gctgttctct ttgacagcct
     8941 ctttttttat gtttccttgt cagatgtctt gttccttttc cccgagagtg attcaaatga
     9001 cacgagcagc ttgtgcaaaa gcctgaagta tagaatgata taaagcacca gagtcatgaa
     9061 ccagatcact gccacattga caacatatgt atctgtcaga cggccaaaga tccgcttttc
     9121 cggtgaatag aaatgagcct ttatgaatgc cggcccggga tccatgaata ttggatccag
     9181 tttcctgacc agttcatttt tgtattcaat gatcttctgt gtttcatttt tggctgtcac
     9241 aaattcttca agtgcaacat ttgagtgatc attcctgagt ttctggaaat atgatttgtc
     9301 tttttcctcc atagtggtga tcagcagatc cttgaggctg tttgcctcat tatagaatct
     9361 tacatagtat cgccttacag cctccacata cccggctgcc tcactgataa tttccggggt
     9421 taccttctcc ggtgttagca tcggggcatt ggcaaagctg atatcgggca ggatcttaag
     9481 ttccttgccg atctcattgg aaacgataag cagattactg ttgaaatcat cgctcctgct
     9541 gccccgttca agatcgagca tcaggatgtc gagcttcccc ttgagctttg agttccagta
     9601 atccttcttg tacctggcat tgctcatatc cttgtcgtac tcatagaaaa gccgttcata
     9661 cctgttgtcc atgaattgct ttaccgcaag ggcttcataa ccccatcgtg ccgtgatcat
     9721 ctctccgtaa acggggatgt cgaccggcga tgaaatgcgc ggattgagct tttcgaattt
     9781 gaccatcacc ccgctcagga gaatctgtgg tatcaccagg aagggaataa ggatgtatat
     9841 tgtaaccacg gtcttgaaac tgtcggatat cagcagcccc atgacgtttg ccgatgccca
     9901 gcaggaaaaa agcaccagcc agtattcgaa tgtcattccc ctgatcccgg tgattgagtt
     9961 tcctatcagc acaaatgaca gtgactggac agccgagata ccgaactgta ccagcatctt
    10021 tgaggccagg tagccgctcc tgctcaggtt aaggaaggat tccctcctga gaatcttcct
    10081 gtcctttatg atttcctccg cacttacagt gagccccatg aagatggcaa caatgaccgc
    10141 catgaagagg tataccggca ggttgctgtt atccatgagc gtgtacccgg gatttttggc
    10201 gcttacatcg aaatacctaa tgaggaaggc aaggaagaat gccagtaccg gagcctcaag
    10261 caatgttatc agcacatact gcagatcggc cgcttttgaa agaatatccc tctgtgcaaa
    10321 aaccaggaac tgcttgtacc atgacggggt cctgaaatta atctcaggaa gggaatcacc
    10381 cttctccaga tgctcctcct tcttttccga ttcatacagt ttccaccatt caccggggga
    10441 aatgcgccgg gtggatgtgg gcctgccgct ttccgtaaaa accttcgtct ccacgatatt
    10501 gaagatctgt tcgggattga catttccgca tgaatggcat tcactttcgc tgtagtcggc
    10561 ctgccctatc ttttttttga agtattcaat cgaatcgacg ggatttccgt tataaatcat
    10621 gtagccgccg gtatcgagga ttatcagctt gtcgaacatt ttgaaaatgt cggatgaggg
    10681 ctggtgaatt accacgaaca ggagtttccc tttgcgcgag agttccttca gcaggtcaag
    10741 tatattctcc gaatcccttg acgaaaggcc cgatgttggt tcatccagga acaggatcgc
    10801 cggctccctg atgagttcaa gcgaaatatt gagccttttc ctctgacccc cgctgatctt
    10861 tttattcagg ggcgaaccta ccttcatgtt cctgatctcg tggagaccga gactcttcag
    10921 aacgccatct accctttcct caatctctgt ttccgacaga ttgccgaaac aaagccttgc
    10981 attgtagtaa aggttctcaa aaacagttaa ttcctcgatc agaaggtcat cctgtgaaac
    11041 atatcc
//
LOCUS       DAOOYG010000086        10974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6399_length_10974_cov_6.628171, whole genome shotgun sequence.
ACCESSION   DAOOYG010000086 DAOOYG010000000
VERSION     DAOOYG010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10974
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6399_length_10974_cov_6.628171"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PK288_08110"
     CDS             complement(<1..633)
                     /locus_tag="PK288_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM18928.1"
                     /translation="MRQLKITKQVTNRDTPSLDKYLQEIGRVDLISPEDEVILARKIK
                     AGDSEALGKLVKANLRFVVSVAKQYQNQGMGLPDLINEGNLGLMKAAQRFDETRGFKF
                     ISYAVWWIRQAILQALAEQARIVRLPVNKIGSINRINRAFARLEQKYEREPSSQEIAE
                     VLEMIPEDVKEALRTNGRTLSMDAPISSEEENTMYDVMQNPDTPTPDKNLI"
     gene            997..2787
                     /gene="rho"
                     /locus_tag="PK288_08115"
     CDS             997..2787
                     /gene="rho"
                     /locus_tag="PK288_08115"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPM18929.1"
                     /translation="MYDILELSNKLLPELRDIARELKIKRIESLKKQDLIYKILGQQA
                     IEATQGRKAPEPDEDIKPEVPEVRHGEPGQRRGKRPRTVKPAFVRSVESVMSVSPDDL
                     QKGEIKDTERAEPVHRSESQEAKPEKTKGDSRIPVWKNDKRNGDKRESNKRLAENRPL
                     KREPVADETIVEATLPLDLDDTLFEQPESSADLIQSEPITEQIQSDVSHEQPSKEEKK
                     EKPYEFEGIIYNTGVLEIMPDGYGFLRSPDYNYLNSPDDIYVSQSQIKLFGLKTGDTI
                     KGSIRPPKEGEKYFPLIKVDEINGRSHDFIRDRVPFDFLTPLFPNEKFTLCAPGEGSL
                     SVRVIDMFAPIGKGQRGLIVAQPKTGKTILLQEIANAIAKYHPDVYLMILLIDERPEE
                     VTDMARNVKAEVIASTFDEPAERHCRIANIVLEKAKRLVECKHDVVILLDSITRLARA
                     FNTTAPASGKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIIATALTETGSKMDDVI
                     FEEFKGTGNMELQLDRKLSNRRIFPSIDIPASSTRREDLLINKNILQKIWVLRNFLTD
                     MNAVEAMEFLRDRLMQTKSNEEFLISMNGS"
     gene            2863..6414
                     /gene="nifJ"
                     /locus_tag="PK288_08120"
     CDS             2863..6414
                     /gene="nifJ"
                     /locus_tag="PK288_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPM18930.1"
                     /translation="MGTKKFITCDGNQAASHVAYMFSEVACIYPITPSSTMAEYVDEW
                     AANGLKNIFGEEVKVVEMQSEGGAAGAVHGALQTGALCSTFTASQGLLLMIPNMYKIA
                     AELLPGVFHVSARTIAAHALSIFGDHSDIYATRQTGFALLASGSVQEIMDLAGVAHLS
                     AIKSRIPFLHFFDGFRSSAEIQKIEMIEMEDMKKLLDWEALKRFRDNAMNPEHPVTRG
                     TAQNPDIFFQAKEAINRFYEPVADIVNGYMKEISKLTGREYKPFTYYGDPEAENIIIA
                     MGSVTDTTKEVIDYLRAKGEKVGLISVHLYRPFSHKYFFNVFPGSVKKITVLDRTKEP
                     GAPGEPLYLDIKDMFYERAERPLIVGGRYGLSSKDTTPAQILSVFENMKLDKPQNNFT
                     VGIVDDVTFKSLPMLPEINVALPGTYSAKFYGLGSDGTVGANKNSIKIIGDTTNKFCQ
                     AYFAYDSKKSGGITTSHLRFGDKPIRSPYLVNTPDFVACHVPSYLEKYDMLKGLKKNG
                     TFLLNSIWDVEETKNKLPDHMKKYMAENNINFYIINATKIAEDIGLGTRTNTIMQAAF
                     FKVANVIPYEKAESEMKTAVYKTYGRKGEDVVNMNYAAIDMGSKVTKVEIPAEWAKIE
                     IKPADDHRNIPAFIREVMEPINAMKGDDLPVSAFIGREDGTFPAGTTAYEKRGIAVNV
                     PEWQAEECIQCNQCSYVCPHAAIRPFLLTEEEAANAPEGSTFIQGIPGVLKQYKFRIQ
                     VSVYDCTGCGNCADICPSKKKALIMKPLETQLDEAPRWTYMHEEVGYKDNIVDKFLNV
                     KNSQFAQPLFEFSGACAGCGETPYIKAITQLFGDRMIISNATGCSSIYGGSAPSTPYT
                     FNQKGHGPSWANSLFEDNAEFGYGLFLGANQLRERIARLMNESMTNGLKPETKAAFED
                     WLRDKDLAEPSRKASEKVIEALENDTSDVAREILSIKQYLVKKSHWIFGGDGWAYDIG
                     YGGLDHVLASGEDVNILVLDTEVYSNTGGQASKSTPAGAVAKFAASGKKIRKKDLGQM
                     AMTYGYVYVAQVAMGANNNQFLKAIKEAEAYPGPSLIIAYSPCINHGLREGMGKTQAQ
                     QENAVKAGYWHLYRYNPLLEKDGKNPFTLDSKEPDWTKFQDFLRSEVRYTSLIKSFPE
                     QAEILFKAAEENSKWRYNSYKRLASMVYEPAVQQTAG"
     gene            6713..7147
                     /gene="ruvX"
                     /locus_tag="PK288_08125"
     CDS             6713..7147
                     /gene="ruvX"
                     /locus_tag="PK288_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPM18931.1"
                     /translation="MGRLLGIDYGTKRIGLAVTDPLNIFASPLATVPNHEFEEYLSGY
                     LKEQSIDAFVVGYPVKMNNQPGDTVRYVDPFIRRLGKLYPGIPVHRVDERLTSRMAFQ
                     AMIGGGLKKKDRMNKGIIDKLSAAIILQSFLEKKIKVKGDEL"
     gene            7201..7773
                     /gene="def"
                     /locus_tag="PK288_08130"
     CDS             7201..7773
                     /gene="def"
                     /locus_tag="PK288_08130"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPM18932.1"
                     /translation="MVYPIVVYGHPVLRKVSEEIGKDYPDLDQLISDMFETMYRSEGM
                     GLAAPQIGKSIRIFIIDATPLAEEEPELADFRQVFINARIVEKSGDVKLMSEGCLSIP
                     NIREEVNREFRIRIQYYDRDWNYHDEIHEGYRARIIQHEYDHLDGILFTDKVSPLRKR
                     LLKGKLNAITKGKFEASYQTILPGQKIKTA"
     gene            7883..9946
                     /locus_tag="PK288_08135"
     CDS             7883..9946
                     /locus_tag="PK288_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPM18933.1"
                     /translation="MLNKEIPFLRIGLPFCLGIISGRCFTPGPGLLGVFTIIVITGFI
                     ASLFFNSRIVNPLYGFAMSIALLVTGLVLYNHEKAGISDLEQVTNEFLCILSEFPEEK
                     GKSTRMVLELKGMASSGSFRSVHGSILVHARKDPAVSQLLPGDILLIRCRPERIMNRG
                     NPCEFDYKFYLENHGIRFQAFIDKNDVLSHIPPQRRKLIYKALIIREKIIDIYRSRGI
                     CCDRLALVAAITLGQKNLLDREQKQYFIKAGVMHIMAVSGLHAVILSLFVQHLLFFLR
                     GRFTFLRRILTMLFLWSFAFVTGLTPSVLRATIMYTFLQTGKLLGRNANNLNSVLASA
                     FFQLLWKPSVLFDAGFQLSYSAVIFIICFYGSIYRLFSIKNWLTDKIWQSVVVTIVAQ
                     AGTLSLTISLFNRFPTYFIITNLLIVPLSNILIITGCLVPLTYPLEFLSRPLAGFLEI
                     LTGITEGLTRQAASLPFSTIDPIGLGTFSAFALSVFIFLLFQSLSDHKNKPLFTPLIA
                     LLIFMISLTYGDITARKTNELIVYNTSCSYAVGIRTGKCMYIFSSCEVVPDEVSRHCA
                     MKNLKIRKEILPGENSVIEAGRCRILVSGYLNNRILEKADPDYVIIYAGPDRSNRPIN
                     ANNRLNTIIFTRSLTSPQLQKGVHDRVRIHHVKRSGAFVTRLDLARGKNQSPGQGRRN
                     VIPLQ"
     gene            10111..>10974
                     /locus_tag="PK288_08140"
     CDS             10111..>10974
                     /locus_tag="PK288_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPM18934.1"
                     /translation="MLFISYFVLLFIDAMRIVIAGAGEVGTHLAKMLSNENHDIILVD
                     TEEDHLKPLDSSLDVLTYVGSATSVNLLRDILQKKTDLFIAVTHWEDTNITSSILAKK
                     FGAVKTIARIDNLDYLEHSTLEFFKSLGIDSLIYPELIAAREVLGLLHETGTTEFMEF
                     CGGKLAMYVQKLDENAPILNKTLEEMSIDHSTDKFRAVAIKRNDKTIIPKGQDRFFLD
                     DTVFVISTNEGIAEMMARSGKKSFEAKRIMILGGSRIGKHVALYMQKSCEVKLIDSDP
                     AKCQALSEILDD"
BASE COUNT         3166 a         2462 c         2482 g         2864 t
ORIGIN      
        1 gatcaggttc ttatccgggg taggtgtatc gggattttgc atcacatcat acatggtatt
       61 ctcctcttcc gaactgatag gagcatccat gctcagtgtc cttccgttgg ttctcagggc
      121 ttccttaaca tcttccggta tcatttcaag aacttcggca atctcctggg aggagggctc
      181 acgttcatat ttctgttcaa gcctggcaaa ggccctgttt atccggttta tggatccgat
      241 cttgttcacc ggcaaacgca ctatccttgc ctgttctgcc agtgcctgga gaattgcctg
      301 ccgtatccac catactgcgt atgaaataaa tttgaacccc ctggtttcgt cgaatcgctg
      361 agcagctttc ataagcccga gattgccctc gttgatgagg tcgggaagac ccattccctg
      421 gttctggtat tgcttagcca ccgaaaccac aaatctgaga ttggctttaa caagtttccc
      481 cagggcttcc gaatcacctg cttttatctt cctggcaagg attacctcat cctcaggtga
      541 tatgagatcc actcttccta tctcctgcaa atacttgtca agagagggcg tatccctgtt
      601 tgtaacctgt ttggtaattt tcaactgacg cattaacagg tcggattagt taaaatttac
      661 atgatatcgg ctcggctaca attttaataa aaataattca gaattccatt tatacaattt
      721 ttttatttgt aacttcgttt atttattcta aattgcaggc attgaacatt tttttcttgc
      781 ttcgctttgc ctggtaataa tcgccgggaa tttctctctt ttttgaatat ttaacttaat
      841 atattattaa ttttgtttaa tattgcgtcg gttttaagat atgtcacaat ttaaggatat
      901 ttgtggcata gtctcgccgg ttcatctgca tttcttggtc cggttttcca acataaacca
      961 atacaatctc agcatcttta tctttattgt gatatcatgt acgacattct cgagttaagt
     1021 aacaagcttc ttcccgaatt aagggatatt gccagggagc ttaaaataaa aagaatcgaa
     1081 tcactcaaaa aacaggatct tatatataag attctggggc agcaggcaat tgaagcgacc
     1141 cagggccgga aagcccctga acctgatgaa gacattaaac cggaagtgcc ggaagtgagg
     1201 catggcgaac ctggtcagag aaggggtaag agaccaagaa cggtcaagcc cgcttttgtc
     1261 cgttctgttg aatcagtaat gtccgtttca cctgacgatc ttcagaaagg tgaaataaag
     1321 gataccgagc gtgcagaacc tgttcacagg tccgaatcac aggaagctaa acctgagaaa
     1381 acaaagggag attcaagaat tcctgtatgg aaaaacgaca aacggaacgg agataagagg
     1441 gaaagcaaca aaaggcttgc cgaaaacagg cccctgaaaa gggagcccgt cgccgacgaa
     1501 acgatagttg aggcaacact tccactggat ctggatgaca ccttgtttga acaacccgaa
     1561 tcatcggcgg acctgattca gagcgagccc attaccgaac agattcagag tgatgtgtcc
     1621 catgaacaac cttcgaagga ggaaaaaaag gaaaaaccat atgaatttga aggaataata
     1681 tacaacaccg gggtacttga aataatgccc gacggttatg gtttcctgag atcgcctgat
     1741 tacaattatc tcaattcccc cgacgatatt tatgtgtcac agtcacagat caagcttttc
     1801 ggactgaaaa cgggcgatac gattaaagga tccatccgtc ctcccaaaga aggagagaag
     1861 tattttcccc tcatcaaggt tgacgagata aacggaagaa gtcatgactt tatccgcgac
     1921 cgggtaccat ttgattttct tactccgctt ttccctaacg aaaaattcac cctgtgtgca
     1981 cccggtgaag gatccctgtc tgtcagggtt attgacatgt ttgccccgat agggaaaggt
     2041 cagcgcggac taattgttgc acagccaaaa acaggtaaga caatattgct acaggaaatt
     2101 gcaaatgcca ttgccaaata tcaccccgac gtgtacctga tgatattact tattgatgaa
     2161 aggccggagg aggtaacgga tatggcccgc aatgtaaagg cagaggttat tgcttcaaca
     2221 ttcgacgaac cggcagagcg gcactgcaga attgcaaaca tagtccttga aaaggccaaa
     2281 cgtcttgtcg aatgcaaaca tgacgtggtg atactactcg attccattac caggcttgca
     2341 agagcattca acactactgc cccggcttcg gggaaagtac tgtcaggagg ggttgattcc
     2401 aatgcattgc acaagccaaa acggttcttc ggagctgccc ggaatatcga gaacggaggc
     2461 tcacttacca ttatcgcaac cgcccttaca gaaaccggtt caaagatgga tgatgttatc
     2521 tttgaagagt tcaagggtac cggtaatatg gaactgcagc ttgacagaaa actttccaac
     2581 aggcgaatat tcccttcaat agatattccg gcatcaagca cgcggcgtga agatctcctt
     2641 ataaacaaaa atatcctgca gaaaatctgg gtgctccgca attttctcac cgacatgaat
     2701 gctgtcgagg caatggagtt cctgagggac aggcttatgc aaacaaaatc aaatgaagaa
     2761 ttcctgatct caatgaatgg cagttaagga acattttttt tgtatctttg cgttttcttt
     2821 aaaaattcat ttgcaactat tatattatct aaactattta acatgggaac gaaaaaattt
     2881 ataacatgcg atggtaatca ggctgcatcg catgtcgctt atatgttcag tgaggttgcc
     2941 tgcatctacc ctatcacccc gtcctcaaca atggcagaat atgtggatga atgggccgca
     3001 aacggattga agaacatctt tggtgaagag gtaaaggtcg ttgagatgca atcggaaggg
     3061 ggagctgcgg gtgcagttca cggggcgctt cagacaggag ccctatgctc tacattcaca
     3121 gcctcacagg gattactttt aatgatcccg aacatgtaca agatagccgc ggagcttctt
     3181 ccgggtgtct ttcatgtaag tgccagaacc atagctgctc atgctctctc aatatttggc
     3241 gatcatagtg acatttatgc caccaggcag acaggatttg cattactggc cagcggcagc
     3301 gtccaggaaa taatggacct tgcaggtgtg gcccaccttt ctgcaatcaa atccagaatt
     3361 ccattccttc atttcttcga cggattccgt tcctcagccg aaattcagaa gatcgagatg
     3421 atcgaaatgg aggatatgaa aaaactactt gactgggagg cgcttaaaag attccgcgac
     3481 aatgccatga atccggagca cccggttacc cggggaactg cccagaatcc ggatatcttc
     3541 ttccaggcaa aagaggcaat aaacaggttt tatgagcctg tggccgatat tgttaacgga
     3601 tacatgaagg aaatatcaaa acttacgggc agggagtaca aaccatttac ttattatggt
     3661 gatccggagg ctgaaaatat tatcattgcc atgggttcgg taaccgatac aacaaaggaa
     3721 gtcattgatt atctcagggc aaagggcgaa aaggtcggcc tgataagcgt acatctctat
     3781 cgtcccttct cccataaata tttctttaat gtatttccgg gttctgttaa gaaaataaca
     3841 gtacttgaca ggaccaaaga gccaggcgca cccggagaac ccctttacct tgatatcaag
     3901 gatatgttct atgaaagggc tgaaaggccc ctgattgtcg gcggtcgcta cggattaagt
     3961 tcaaaggata ccacaccggc acagattctt tccgtttttg aaaacatgaa gcttgacaag
     4021 cctcaaaata acttcacggt tggcattgtc gatgatgtta ccttcaaatc cctaccaatg
     4081 ctccctgaga tcaatgtggc acttcccgga acctactctg caaaatttta cggacttgga
     4141 tcggatggaa cggttggtgc aaacaaaaac tcaataaaaa tcataggaga caccaccaat
     4201 aaattctgtc aggcttactt tgcctacgac tcaaaaaaat caggcggcat aacaacgtca
     4261 cacctgcgct ttggcgataa acccattcgt tcgccatatc ttgtgaatac tcctgatttt
     4321 gttgcatgcc atgttccctc atacctcgaa aaatatgata tgctcaaggg acttaaaaag
     4381 aacggcacat tcctgcttaa ctccatctgg gatgtcgagg aaacgaaaaa caagcttccc
     4441 gatcacatga agaagtacat ggctgaaaac aacatcaact tttacatcat caatgcaacc
     4501 aaaattgctg aagatatcgg cctgggaacc cgaaccaata caattatgca ggctgcattc
     4561 ttcaaggtgg caaatgtgat tccctatgag aaggctgaat cggaaatgaa aacggcggta
     4621 tataaaactt acggcaggaa aggcgaagat gttgtaaata tgaactatgc cgcgatcgac
     4681 atgggaagca aggtaacaaa agtagagatc ccggcagaat gggcaaaaat cgaaattaag
     4741 cctgcagacg accatcggaa cattcccgca ttcatccgcg aagtcatgga gccaataaat
     4801 gcaatgaaag gtgacgatct gccggttagt gcttttatcg ggcgtgaaga tggtaccttc
     4861 ccagcaggaa caactgctta tgagaagcgc gggattgcag taaacgtgcc cgaatggcag
     4921 gccgaagaat gcatccagtg caaccagtgt tcatatgtct gtcctcatgc tgcaatacgt
     4981 cccttccttc taacagagga ggaagcagcc aatgcacctg aaggaagcac attcatccag
     5041 ggtattcccg gcgttctcaa gcaatacaaa ttcaggatcc aggtcagtgt ttacgattgc
     5101 accggatgcg gtaactgtgc cgatatctgc ccctcaaaga aaaaggctct gataatgaaa
     5161 cccctggaaa ctcagcttga tgaagcacca cgatggacct atatgcatga agaggtaggt
     5221 tacaaggata atattgttga caaattcctg aatgtaaaga acagccagtt tgcccagcct
     5281 ttgttcgagt tttcgggtgc ctgtgcagga tgtggtgaaa caccttacat taaagctatt
     5341 actcagctgt tcggcgacag gatgattata tcaaacgcaa ccggatgttc ctcaatttat
     5401 ggcggatcgg ctccgtcaac accgtatacc ttcaatcaga agggacacgg accttcatgg
     5461 gcaaattctc ttttcgaaga caatgccgag ttcggatacg ggctcttcct tggtgcaaac
     5521 cagctcaggg aacggattgc ccgccttatg aatgagtcaa tgacaaacgg gctgaaacca
     5581 gaaaccaagg ccgcttttga agactggctg agagacaagg atcttgctga accttccaga
     5641 aaagcttccg agaaggtcat tgaagccctt gagaatgaca catcagatgt tgcaagggag
     5701 atattgagca tcaagcagta tctcgtcaag aaatcgcact ggattttcgg tggcgatggc
     5761 tgggcatatg atattggtta cggcggcctc gaccacgtac ttgcatcagg ggaagatgta
     5821 aatatcctgg tacttgatac tgaggtttat tccaacacag gcggccaggc atcaaaatca
     5881 acccctgcag gagctgttgc caagtttgcg gcttcaggaa aaaagatccg caaaaaggac
     5941 ctcggacaaa tggcaatgac ctatggttac gtttatgttg cccaggtggc aatgggagcc
     6001 aacaacaacc agttcctgaa ggcaataaag gaagcagaag cttaccccgg cccttcactt
     6061 attattgcct actcaccgtg tatcaatcac ggattacggg aaggcatggg caagacccag
     6121 gcccagcagg aaaatgctgt taaagccggt tactggcatc tttacagata caacccgctt
     6181 cttgaaaaag acggaaagaa tccgtttaca ctcgattcca aggagccaga ctggaccaag
     6241 ttccaggatt tcctccgttc ggaagtccgg tacacctccc ttatcaaatc cttccccgag
     6301 caggcagaga tattgttcaa ggctgccgaa gagaattcaa aatggaggta taattcatac
     6361 aagcgacttg cttccatggt atatgaaccg gcagtgcagc aaactgcagg gtaaaaaata
     6421 aggaagggtg tttcatttgt tctatgaaac accctttttt aataatgccc cgcaggaacg
     6481 ctgagatccg tatcttcagc cttcgctgtt tcactacccc ggttcatgga aaccaaagtt
     6541 aaccagataa ccgcggttaa gggaatatcg gtacatcatt tgcctgattt caggctattc
     6601 ctgactgtcc ggcagtcctt ctgccgtatg ctcttgctcc tggcctttaa gcttcagcct
     6661 ctggtttttg cacttccgca gatttctttg tacttttgca acaacaaaaa agatgggcag
     6721 actgcttgga atagactatg gaacaaaaag gatcggtttg gcagttaccg atcccctaaa
     6781 tatttttgca tcacccctgg caactgtccc gaatcatgaa tttgaggaat acctttcggg
     6841 atacctcaaa gaacaatcca tcgatgcatt tgttgtagga tatccggtga aaatgaacaa
     6901 tcaacccggc gatactgtga ggtatgttga tccgtttatc agaagactcg ggaagcttta
     6961 tccgggaata ccggtacacc gtgtcgatga gaggcttacc tcccggatgg ccttccaggc
     7021 catgattggc ggaggtctga aaaaaaaaga cagaatgaat aagggcatta ttgacaaatt
     7081 gagtgctgca attattcttc agtcatttct tgaaaaaaaa attaaggtaa aaggcgatga
     7141 attgtaacag gaccatccgc ctggaaagaa ttaaaactct gaattaataa cgatttgctg
     7201 atggtttatc cgattgtagt atacggacat ccggttctaa ggaaggtttc ggaagaaatc
     7261 gggaaggatt accctgacct tgaccagttg atatcagaca tgtttgaaac aatgtacagg
     7321 tccgagggga tgggtctggc tgcaccacag ataggaaaat caatccgaat atttattatt
     7381 gatgccaccc cgcttgctga ggaggaaccg gaacttgctg atttcaggca ggttttcatc
     7441 aatgcaagga tcgtggaaaa atcaggcgat gttaaactta tgagtgaagg atgtctgagc
     7501 attccaaaca taagggagga agtgaaccgg gagttccgga tcaggataca atattatgac
     7561 agggactgga attatcatga tgaaatacat gagggctacc gggccaggat cattcagcat
     7621 gaatatgatc atctcgatgg gatattgttc acggacaaag tcagccccct gagaaaaaga
     7681 ctccttaaag gtaaactcaa cgccataacc aaaggcaaat ttgaagcttc atatcagaca
     7741 atacttcccg gtcagaaaat aaaaacggct taggcccggc aatggttaca gggtaaatat
     7801 gccattgcat ttttagtctt acggaattta attcaactgc aacttttttc ctatctttat
     7861 tcagtcttca tcgctgcaga ctgtgctgaa taaagagatc cctttcctgc gtatcggcct
     7921 gccgttctgt ttgggaatta tttcaggccg ttgcttcact ccaggacctg gcttactggg
     7981 tgttttcaca attatcgtta tcactggttt catagcaagc ctgttcttca atagccggat
     8041 tgtcaatcca ctatatggct ttgccatgtc cattgccctt ctggtgacag gcctggtact
     8101 ctataatcat gaaaaggcag gaataagcga tcttgaacag gttacaaatg aatttctttg
     8161 tattctttca gaatttcccg aggagaaagg aaaaagtaca agaatggtac tggaactgaa
     8221 agggatggca agcagcggtt cattcaggtc cgtgcatggt tcaatcctgg ttcatgccag
     8281 gaaggatcct gcagtatcac agcttctccc cggtgatata ttattaatcc gatgccggcc
     8341 ggagagaata atgaacaggg gaaatccatg cgagtttgat tacaaatttt acctggaaaa
     8401 tcacgggatc aggttccagg catttattga taaaaatgat gttctatctc atattccgcc
     8461 gcaaagaagg aaattaattt acaaggcgct cataatacgg gaaaaaataa tagacattta
     8521 caggagccgc ggcatctgct gcgacaggct cgccctggtg gcagcaataa cccttggtca
     8581 gaaaaacctt cttgaccgcg aacagaaaca atatttcata aaggcggggg taatgcacat
     8641 aatggctgtt tcaggcctgc atgcggtgat actcagtttg tttgtccagc acctgctgtt
     8701 tttcctcagg ggcaggttta cttttttaag gagaatcctg acgatgctgt tcctttggtc
     8761 gtttgcattt gttacaggcc tcacaccatc ggttctgaga gcaacgataa tgtatacctt
     8821 cctgcagaca ggcaaactgc tggggagaaa tgccaataac ctgaactcag ttctggcatc
     8881 ggcatttttc cagctcctgt ggaaaccatc cgtcctattc gatgccggat tccagctgtc
     8941 atactctgcc gtgatcttca taatatgctt ctatggaagt atataccggc ttttcagcat
     9001 aaagaactgg ctgaccgaca aaatctggca gtcggtagtg gttacgattg ttgctcaggc
     9061 cgggacactt tcattaacaa tttcactttt caaccggttc ccaacctact tcattattac
     9121 aaatctttta atagtacctc tctcaaatat tctgataatc acaggttgcc tggttccgct
     9181 gacctatccg ctggaattcc tatccaggcc tctcgccgga tttcttgaaa tactcaccgg
     9241 catcaccgaa gggctcaccc ggcaggctgc ttctcttccg ttttcaacta ttgatcccat
     9301 agggctggga actttcagtg ctttcgccct gtcagttttc attttccttc ttttccaaag
     9361 tctgtctgat cataagaata aaccgctgtt cactcccctg atcgctttgc tgatttttat
     9421 gatttcactc acgtacgggg atatcaccgc aagaaaaacc aatgagttga tcgtatataa
     9481 cacttcctgt tcatacgctg ttggcataag aacgggtaaa tgcatgtaca ttttcagcag
     9541 ttgcgaggtt gttcctgatg aggtttcaag gcattgtgcc atgaaaaatc ttaagatcag
     9601 gaaggaaata cttccggggg aaaactcagt tatcgaagct ggcaggtgca gaattctggt
     9661 gtcgggatac ctcaacaaca ggatcctgga aaaagccgac ccggattatg taatcattta
     9721 cgccggccct gacaggagca acaggccaat caacgcaaac aacaggttaa atacaatcat
     9781 atttaccagg tcattaactt ccccgcagct acagaaaggt gtgcatgaca gagtcaggat
     9841 ccaccatgtt aaaagatcag gtgcctttgt tacccggctt gacctggcaa ggggaaagaa
     9901 ccagtcaccc ggtcagggca ggaggaatgt cattcctttg cagtaatcct tgcaatgagc
     9961 ctggtacaaa caagcctggt attttgatcc gggaagtcca aaccaatatt tccggccagg
    10021 caggctcagt tttttgcatg cttcggtttg tttgtccgga aggccccggg gtcagaacat
    10081 gtaaaataca gccataaaaa cttgagtttt atgctattta tatcttattt tgtcttgcta
    10141 tttatcgatg ccatgagaat tgtaattgcc ggagcaggag aagtaggaac tcaccttgca
    10201 aaaatgctgt cgaatgaaaa ccacgatatc atccttgttg ataccgagga ggatcatctc
    10261 aaaccccttg actcatcact tgatgttctc acctatgtcg gatcggcgac atccgtaaac
    10321 cttctccggg acatactgca gaaaaagact gacctgttca ttgcagtcac tcattgggag
    10381 gacacgaata tcacctcatc aattctggcc aaaaagttcg gggcagtaaa aacgattgca
    10441 cgcatcgaca atcttgatta cctggaacac tcgacccttg aatttttcaa gtcgcttgga
    10501 attgactcat tgatttatcc tgagctcatt gccgccaggg aggtgctcgg cctgcttcat
    10561 gagacaggaa ctactgaatt catggagttc tgtggaggaa agctcgccat gtatgttcag
    10621 aaactcgatg aaaacgctcc tatcctgaac aaaaccctgg aagaaatgtc aattgatcac
    10681 agcaccgata aattcagggc tgtagccatt aaacgaaatg ataagacaat tattccaaaa
    10741 gggcaggatc gttttttcct cgacgatacg gttttcgtta tctctacaaa tgaagggatt
    10801 gctgaaatga tggccagatc gggcaaaaag agctttgagg ctaaaaggat aatgattctt
    10861 ggtggcagca ggattggcaa gcatgtcgca ctttacatgc agaaaagctg tgaggtcaag
    10921 cttattgatt ctgatccggc aaaatgccag gctctgtccg agattcttga tgac
//
LOCUS       DAOOYG010000087        10795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6552_length_10795_cov_7.890875, whole genome shotgun sequence.
ACCESSION   DAOOYG010000087 DAOOYG010000000
VERSION     DAOOYG010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6552_length_10795_cov_7.890875"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..1372
                     /locus_tag="PK288_08145"
     CDS             401..1372
                     /locus_tag="PK288_08145"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPM18935.1"
                     /translation="MRTIAIVAGGDSPEYEISVKSAGEVYKVLSAVYSAYIIMIRGFD
                     WYWEDSRGRCHIVDKNDFALRLDDRKIRFDAVFIAIHGSPGENGLLQGYFDMMRIPYT
                     SCSAFCSALTFNKQACKLYLKEYGIPMAKSVLIRRSDKINPDEIITSVGLPCFVKPND
                     SGSSFGVTKVKTGDELLPAIMKAFEESNEVMAETLMKGREVACGVVKTSKRILVLPVT
                     EIISKNEFFDFEAKYTPGKSVEITPADLTQALTDKIRELSSRIYDLLGCRGIVRVDFI
                     VAGDDPLFLEINSVPGMTRESLVPKQAEAAGISLSDIYSMVIEDLFT"
     gene            complement(1446..2849)
                     /locus_tag="PK288_08150"
     CDS             complement(1446..2849)
                     /locus_tag="PK288_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005636180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HPM18936.1"
                     /translation="MRKPLSIILSFMLVVSSCGPGGASRQKSKGPKASSEDKYPVQLI
                     TVDPGHFHAALVQKSMFPQVSPDVHVYAPDGPDLQQHLDRIASYNKRPVDPTSWNEIV
                     YRGNDFFEKMLSEKPGNVVVLSGNNKKKAEYISRSIDAGLNVLADKPMIIYPEDFPIL
                     EKAFQTAEEKGVLLYDIMTERFEITTILQKILSQNENVFGKLTTGSRKDPAVSKISVH
                     HFSKLVSGAPLIRPAWYFDVEQQGEGIVDVTTHLVDLIQWACFPDQILNPADINMVSA
                     RRWPTVITRDQFRGVTGYDDFPEFLLKDVRDGNLNVFANGEMVYQIKGVWAKVSVEWH
                     YQAPPGGGDTHYSVMKGTGCDLVIRQGREEKFIPTLYAENIRGRDIESFTTDLREVLA
                     ALPYDSLGVEPFGKSALRINIPDKYRVSHEEHFGQVASKFLLYLNEGSLPYWEVPGMI
                     TKYYTTTSALKMALEKQ"
     gene            complement(2993..4030)
                     /locus_tag="PK288_08155"
     CDS             complement(2993..4030)
                     /locus_tag="PK288_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPM18937.1"
                     /translation="MQDSSWNDLTGLLDGKNPGYRPVGFIIDSPWIPGWYGISMLDYY
                     TSDELWLESNLAAVQRFPDVWFMPGFWPEYGMCTEPSAFGSRMIFSEKSLPHAEKIIS
                     DISDIVRLPHPDVRTDGLLPFVINRLKNCNKAISDAGHQVRFAVSRGPFNIASFLLGT
                     TELMMAVAIDRENTHKLLNRITDFICDWLSWQRECFPSVDGVLILDDIIGFVGEAEFI
                     EFVIPNLKRIFDSTGFRIKFLHNDADGLITARHLSDMGVNMFNFSFNHSLSEIRILAG
                     EDIILAGNIPPRDVLAAGSASDVAAAVKTALAEVPDHSRIVWSAGGGMPPGVSSENIR
                     AFVETVRDMAI"
     gene            complement(4161..7046)
                     /gene="gcvP"
                     /locus_tag="PK288_08160"
     CDS             complement(4161..7046)
                     /gene="gcvP"
                     /locus_tag="PK288_08160"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HPM18938.1"
                     /translation="MLSDKFENRHNGPRGAEVTRMLETVVVASLDELIDKTIPASIRL
                     KKPLELPAAMTEYEYLGHIRQLGSKNKLFRSFIGQGYYGVAPLSVIVRNVLENPSWYT
                     SYTPYQAEISQGRLEALLNFQTMIVDLTGMEIANASLLDESTAAAEAMIMMFNARSRE
                     AIRAGINRFFVSNDTFQQTIAVLETRAEPLGIELVTGRFDEVAIDGTFFGALVQYPAA
                     SGQIEDYREFAGKVHASGALLGVAADLMSLALLSPPGEWGADVVVGSNQRFGLPMSYG
                     GPHAGFFATKDELKRSMPGRIIGVSVDAQGNHALRMALQTREQHIKRERATSNICTAQ
                     ALLATMSGMYAQYHGPEGLKNIASHISRSACTINGILKDLGYKQANTSFFDTLRIILP
                     DEGSVEKIRNLALESRINFHYPGGNIVMLSTDEITTLEEVNLITSVFARAAGLKPRPA
                     DSFCSCGSAYEAFTRKSEFLKASVFNRFHSETEMMRYIKMLERKDFSLTHSMISLGSC
                     TMKLNPATSMFAMSWPEFGNIHPYVPATQAEGYYQVMDELGEALKKITGFSAISFQPN
                     SGAAGEYTGLLIMREYFKQRGEGNRNVVLIPSSAHGTNPASAVMAGMQVVVVECDEGG
                     NISLDDLQAKAEQNRENLAGIMITYPSTHGVYEEAVVEMIGIIHQNGGLVYMDGANMN
                     AQVGLTSPGYIGADLCHLNLHKTFAIPHGGGGPGMGPVLVNDKLAEFLPTHPHVQTGG
                     SSGIKAVASAPFGSAHILTISHAYVMMMGPEGLTRATALAILNANYIAAALKGWYKTL
                     YTGNMGFVAHEMILDCRNFKTETGITESDIAKRLMDYGFHAPTLSFPVHGTLMVEPTE
                     SESLYELDRFIEAMKSIWEEIMEIKEGRADRNDNVLRNAPHTFRVAAADKWEHGYGRE
                     KAVFPLRWVTENKFWPSVGRVDDGYGDRNLVCTCDPIDSYRMETLKF"
     gene            complement(7432..8073)
                     /locus_tag="PK288_08165"
     CDS             complement(7432..8073)
                     /locus_tag="PK288_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM18939.1"
                     /translation="MKIYTLVFNPIEVNTYIIADPSGDCIIIDCGCYYGHEFDEMDSF
                     IQDNKLKPVLLLNTHCHLDHIFGNGWMLERFGIRSHYCREDDYNRIDAPVHARLFGLN
                     MELPPEPADYLEDGQIVSHGQIRLTALHVPGHSSGSLSFYSSDLKAVFTGDALFAGSI
                     GRTDLEGGNFETLIASIRNKLFSLPDDTIVYPGHGECTTIKVEKETNPYFIKS"
     gene            complement(8191..8838)
                     /locus_tag="PK288_08170"
     CDS             complement(8191..8838)
                     /locus_tag="PK288_08170"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPM18940.1"
                     /translation="MTDSFEARGKRKKLVDELRRKGITDEEVLRAINTVPRHAFMDPA
                     FLNHAYADKAFPISSGQTISQPYTVAVQTSLLQVRKRDKILEIGTGSGYQAAILAEMG
                     AKVYTIERFRELHLKAQHTLSLLGYSATFFFGDGYEGQPQYGPFDGIIVTAAAAEVPE
                     KLMRQLKIGGRLVIPLGSATSQTMTLVVRTEENKFDYSSHGYFIFVPMIKGIVKS"
     gene            complement(9027..9587)
                     /gene="thpR"
                     /locus_tag="PK288_08175"
     CDS             complement(9027..9587)
                     /gene="thpR"
                     /locus_tag="PK288_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPM18941.1"
                     /translation="MIKRTFIAVKIETTTETERLVANLRSLLGNENIKWADIRNIHVT
                     LAFMGDTPVVKIAPITQLIKGKCAGFGKFDFMLAGTGIFRNFRDPRIIWAGIKPQDGL
                     IKLRALITECLEAGDVGFDGKPFVPHLTLGRLKNIKDIENLRRVLEKYRNIEFQKVWI
                     NEVVLFESILKPAGPVYIPLATLPLS"
     gene            complement(9603..10217)
                     /locus_tag="PK288_08180"
     CDS             complement(9603..10217)
                     /locus_tag="PK288_08180"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HPM18942.1"
                     /translation="MKLVIPILLFSFSLEITLAQTDSSIAGNDTLPRNFYLLHTKERN
                     GESLPEIDLKEVVIIGRQSTSKKFPFRKYERLIYNLKKIYPYGIVVRARLEQVNHELM
                     NIPDEKERKKYLRQVEKNVFREYEDDVRNMTITQGKLLIKLIDRETRNTSYELIRFYR
                     GNLSASFWQAIARIFGTNLKAEYDPYGEDAVMEIILQEIEAGRL"
     gene            10546..>10795
                     /locus_tag="PK288_08185"
     CDS             10546..>10795
                     /locus_tag="PK288_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18943.1"
                     /translation="MALNVNDLEDQSRYRQLIKIPYDELIPFILDYLRRKSGLIVFFW
                     SACILTLGLALTVRVNLSGYFPYKYLLLHSILGLVVFPL"
BASE COUNT         2860 a         2613 c         2305 g         3017 t
ORIGIN      
        1 tgggtttcca cattacccca ttgacttatt actccttcct gaagcgtaac catctgatta
       61 taggattttg tcacccacga tacaagacct gttatcacaa gtgccagaaa aatacctgct
      121 ataagccaaa tcaaacaacc ttttttcatt ttcgcggttt ttataatttc atcaaaggta
      181 attattattg ataaattggg atgatacagg agctctggat tattcaaaca gatatccagg
      241 cccgacaatt agctttacca gcctgcctcg tgcttaccgt tgagaatcca atcaaaaaaa
      301 agccccctta taaagcatct aataaggcat cattttaatt tcattaagtg aaccatcttc
      361 caccggtaaa atatgtaatt ttacagttta ttttttacag atgagaacaa tagctattgt
      421 tgcaggaggc gactctcctg aatatgaaat atcagtaaag agcgcaggcg aggtttataa
      481 agtattatcc gcggtttatt ctgcatatat cataatgata aggggtttcg actggtactg
      541 ggaagatagc aggggacgtt gccacattgt tgacaagaat gattttgcac tcagactgga
      601 tgaccggaaa ataaggttcg atgcggtttt tatcgcaatc catggttccc cgggcgaaaa
      661 cggactgtta cagggatact tcgatatgat gcgcataccc tatacaagct gcagtgcatt
      721 ctgttctgcc cttactttca acaaacaggc atgcaagctt tatctgaagg aatacggaat
      781 cccgatggcg aaatcagtcc ttattcggcg ttcagataaa attaaccctg acgagataat
      841 tacgagtgta ggtctgcctt gcttcgtcaa acccaacgac agcggttcaa gcttcggcgt
      901 aacaaaagtt aaaaccggtg atgagttgtt gccggcaata atgaaagctt tcgaagaaag
      961 caatgaggta atggcagaaa cccttatgaa aggcagggaa gtggcatgcg gtgtggtaaa
     1021 aacttcaaaa aggatcttgg tgcttccggt aacagaaata atcagcaaga atgaattctt
     1081 tgactttgaa gcaaaatata ctcccggtaa atccgtcgaa attactccgg ctgatctcac
     1141 ccaggctctt acagacaaga tccgggagct tagctcaagg atttatgatc tgctcggatg
     1201 caggggaatt gtcagggtcg acttcattgt cgcaggtgat gatcctcttt tccttgaaat
     1261 caattcagta ccgggaatga cccgggaaag tcttgttcca aagcaggctg aagctgcagg
     1321 gatatcttta tcagatatct attcaatggt aattgaagac ctttttactt aatgtcgtaa
     1381 gtacagggtt cgcctcataa tcagttattc cggcctttca actgtactcc ggcaccggaa
     1441 gggcatcact gtttttccag tgccatcttc aacgcgctgg tagttgtgta atattttgtt
     1501 atcattccgg gcacttccca gtaaggaagg cttccctcat tgaggtaaag caggaatttt
     1561 gaggcaacct gtccgaaatg ttcttcatgc gacaccctgt atttatccgg aatattgatc
     1621 ctcaaagccg atttcccgaa aggttcaact ccaaggctgt cgtaaggcaa tgctgcgaga
     1681 acctccctga gatcagtggt aaagctttca atatccctgc ctcttatgtt ctctgcatac
     1741 agtgtcggaa tgaatttctc ctcacggccc tgcctgataa caaggtcaca tccggtgcct
     1801 ttcatcactg aataatgcgt gtcacctccg ccgggaggtg cctggtagtg ccattcgacc
     1861 gaaacttttg cccatactcc cttaatctgg tataccattt caccatttgc gaatacgtta
     1921 agattgccgt cccggacatc cttcagcagg aactccggaa aatcatcata acccgtaact
     1981 cccctgaatt gatcccgtgt gatgacagtg ggccatcttc ttgccgatac catgtttata
     2041 tcagcaggat tgaggatctg atcggggaaa catgcccact ggatcagatc aacaagatga
     2101 gtggtaacat caacgattcc ctctccctgc tgttcaacat caaagtacca tgccggacgt
     2161 atcagcggag ctccggaaac aagtttcgaa aaatgatgta cgcttatttt cgaaactgcc
     2221 gggtcttttc tggatcctgt tgtcagttta ccgaacacgt tttcattctg cgacaggatt
     2281 ttttgaagga tcgtggttat ctcaaacctt tccgtcatta tgtcgtacag cagaacgcct
     2341 ttttcttcag ctgtctgaaa ggctttctcg agaatgggaa aatcttccgg gtagataatc
     2401 attggtttgt cagccagaac attcaaacct gcatcaatgg accgcgaaat atattcagcc
     2461 ttcttcttgt tgttccctga cagtacgact acattgccgg gtttctccga cagcattttc
     2521 tcaaaaaaat cattgcccct gtaaacaatt tcattccacg atgtcggatc aaccggcctt
     2581 ttgttgtatg atgctatcct gtcaagatgc tgctgcaggt ccggaccgtc aggtgcataa
     2641 acatgcacat ccggtgaaac ctggggaaac atcgatttct gtacaagtgc agcatggaaa
     2701 tgaccagggt caacagtgat aagctgtacg ggatatttat cttccgaaga tgctttcgga
     2761 cctttgcttt tctgcctact ggctccgcca ggcccacagg atgacactac tagcatgaaa
     2821 gaaagaatta ttgatagagg ctttctcatt gtcggtttat ttaaaataat tattattcgt
     2881 taaagcttaa agtgactaaa gtaattaaaa agtttggagt gagtcggtgc aggcagcact
     2941 taatgtaata aatttcgtcc tgacccggta ctccggacga atccagcatc ccctatatag
     3001 ccatgtccct gacagtttcc acaaatgccc tgatattttc gctgctgaca cccggcggca
     3061 tacctccacc ggctgaccag acaatccggc tatgatcagg gacctctgcc aatgctgttt
     3121 ttactgcagc agcaacatcg ctggcggatc cggctgccag cacatccctt ggcggaatat
     3181 ttcctgccag aattatatct tctcctgcca ggattctgat ctcagacagt gaatggttaa
     3241 aggagaaatt aaacatattc acgcccatgt ctgaaaggtg ccttgccgtg atcagtccgt
     3301 cggcatcatt gtggagaaat tttatcctga agccggttga gtcaaagatc cgtttaagat
     3361 taggtatcac aaactcaatg aattcagcct ctcctacaaa accaataata tcatcgagga
     3421 tcagtacgcc atcgacggat ggaaagcatt ctctttgcca tgaaagccag tcgcaaataa
     3481 aatcagtgat cctgttcagt aacttgtgag tattttcccg gtcaattgcc actgccatca
     3541 taagctcggt ggtacccagc aggaagctgg caatattgaa tggcccgcgt gaaacggcga
     3601 atcgcacctg atgtccggca tcactgatag ccttattgca gtttttaagc ctgtttatta
     3661 cgaatggcaa caagccgtcc gttctgacat caggatgggg caaccttaca atatcagaaa
     3721 tatcagatat tattttctct gcgtggggaa ggcttttctc cgaaaaaatc attcgtgaac
     3781 caaaagcaga tggttcagtg cacattccat attccggcca gaaaccaggc atgaaccaaa
     3841 catccggaaa ccgttgtact gctgccagat tcgattcaag ccacaattca tcggaggtat
     3901 aataatcaag cattgatatg ccgtaccacc ccgggatcca cggactgtcg ataatgaagc
     3961 cgacaggcct gtaacccggg tttttaccgt cgagcagacc agtcagatca ttccaggagg
     4021 agtcctgcat tgcaataacg ttttaaatca ctatccctgc agctttccat atggttacac
     4081 cgcatctatc gcccgtgcag gatttttttc tttcactttt ccgggcccgg gagctcaggt
     4141 agaatatcag gcctgatctc tcagaatttc aaggtttcca tcctgtagga gtcgataggg
     4201 tcgcaggtgc agacaagatt gcggtcgcca tagccgtcat ccaccctgcc caccgatggc
     4261 cagaacttgt tttcggtgac ccatctgagc gggaaaactg ctttttccct tccataacca
     4321 tgctcccatt tatctgctgc agcaaccctg aaggtgtgag gagcattcct caacacgttg
     4381 tcattcctgt cggccctgcc ctctttaatt tccatgatct cttcccatat tgatttcatg
     4441 gcttcaatga atctatccag ttcgtaaagt gattccgact cggtaggttc caccatcagg
     4501 gtaccatgga cgggaaatga aagggtaggt gcatggaaac cgtaatccat gagccgcttt
     4561 gcaatatccg actcggtaat ccctgtctct gtcttgaagt tcctgcaatc gaggatcatc
     4621 tcatgcgcga caaatcccat gtttccggtg taaagtgtct tgtaccatcc tttaagtgca
     4681 gcagcaatat aatttgcatt cagtatggcc agggctgttg cccgggtgag gccctcagga
     4741 cccatcatca ttacataggc atgggatatt gtcagtatat gtgcacttcc gaatggagct
     4801 gaagccactg ccttaatccc gctggatccc ccggtttgaa catggggatg cgtgggaaga
     4861 aactctgcca gtttgtcgtt gacaagtacg ggacccattc ctggcccacc gccaccatga
     4921 ggtatcgcaa aggtcttgtg aaggttcagg tggcaaagat cggccccgat ataacccgga
     4981 cttgtcagtc cgacctgtgc attcatattg gcaccatcca tatatacaag gcctccgttc
     5041 tggtgtatta tccctatcat ctccacaaca gcttcttcat ataccccgtg agttgatgga
     5101 taggttatca tgatgccggc caggttctcc ctgttctgtt ccgcttttgc ctgcaggtca
     5161 tccaggctaa tattgccacc ctcatcacac tcaaccacta ccacctgcat accggccatc
     5221 acggcacttg ccggatttgt tccatgcgcc gaggaaggaa taaggactac attcctgttg
     5281 ccttctcccc tttgtttaaa atattcccgc ataatcagca gcccggtata ttcaccggct
     5341 gccccggaat taggctggaa gctgattgca gaaaatccgg tgatcttctt cagtgcttca
     5401 ccgagctcat ccattacctg atagtatccc tcagcctggg tagccggaac atacgggtga
     5461 atattgccaa attcgggcca gctcatggca aacatcgaag ttgcaggatt cagcttcatt
     5521 gtacaggagc caagtgatat catgctgtga gtcagtgaaa aatctttcct ttcaagcatt
     5581 ttgatatatc tcatcatctc agtttcgcta tggaacctgt tgaaaaccga agctttaaga
     5641 aattccgatt tcctcgtaaa agcctcataa gctgaaccac aactgcagaa cgaatctgcc
     5701 ggacgaggct tcaggcctgc agccctggca aatacggaag tgatcaggtt cacttcctca
     5761 agagtggtta tctcatctgt gctaagcatt acgatgttgc ctccggggta gtggaaattg
     5821 atcctgcttt ccagtgcaag gttgcggatc ttttccactg aaccctcatc aggaaggatt
     5881 atccttaagg tatcaaaaaa gctggtattt gcctgtttgt atccaagatc cttcagtatc
     5941 ccgttgattg tacaagctga tctgctgatg tgagaagcaa tattcttcag tccctcaggc
     6001 ccgtggtact gtgcatacat acccgacatt gttgcaagaa gtgcctgtgc ggtgcatatg
     6061 tttgaagtgg ccctttcacg cttaatatgc tgctcccgtg tctggagtgc cattctcagg
     6121 gcatggttac cctgggcatc caccgacact ccgataattc tgcccggcat gcttcgcttc
     6181 agttcatcct ttgtggcaaa gaatcctgca tgcggaccgc cgtagctcat cggaagtccg
     6241 aaccgctgat tggaaccgac caccacatcg gcgccccatt caccgggagg agagagtaag
     6301 gcaaggctca taaggtctgc tgccacgcca agaagcgcac cggaagcatg aaccttcccc
     6361 gcaaattccc ggtaatcctc tatctgaccc gatgcggccg gatattgaac aagcgccccg
     6421 aaaaacgttc cgtcgattgc aacctcatca aatctgccgg ttaccagttc gatcccaagc
     6481 ggctcagccc gtgtttcaag aacagcaatc gtctgctgga aggtgtcatt gctgacaaag
     6541 aaccggttta ttccagccct tattgcttcc cttgatcgcg cattgaacat cattatcatt
     6601 gcttccgccg cagccgtcga ctcatcgagc agcgaggcgt tggcaatctc cattccggtc
     6661 agatcaacta tcatggtctg gaaattcagc agggcttcca gcctaccctg tgagatttcc
     6721 gcctgatagg gagtgtagga ggtgtaccac gacgggttct ccagaacatt cctcactata
     6781 acggaaaggg gtgcaacacc ataatatccc tgtcctataa aagatctgaa aagcttgttt
     6841 tttgatccca gctgcctgat atgaccgaga tattcatatt cagtcattgc agcaggaagt
     6901 tccaaaggtt tcttcagcct gatagaggca gggatagtct tgtcaatcag ttcatccagg
     6961 gaagcgacaa cgactgtttc aagcattcgg gtgacttcag ctccgcgggg gccattgtga
     7021 cggttttcaa atttgtctga aagcatataa ttgatatttt ggtcatcacc gggatgggta
     7081 ttctagctgt attccaaaac aggaaatgag tatctgctat tcatcttcac cacaaaatca
     7141 atcatcctaa caaaaatccg taaactttgg ttcatacaac ttactgcaat ccggagtaac
     7201 cgggaaaagt ctttacctgt ccgcaaagga tgattcttag acactgtgga tccctttggt
     7261 ttccggataa tcctttacat ctccggaaat actggttact tcattcaggc aaaaaaatca
     7321 atttcccgtt acaagtactg ttcattatga aaaaccggaa cccggaatca cttctccggt
     7381 caactcatat gcgaaaatag caataagtta ctattttaaa aaccaggctt gtcagctttt
     7441 aataaaatag ggatttgtct ctttttcaac tttaatggta gtacactctc catgtccggg
     7501 gtagactatg gtgtcgtcgg gaagtgaaaa taatttgttc ctgatgctcg cgattagggt
     7561 ctcaaaattt cccccctcga ggtccgttct tccgatactg cctgcaaaga gggcatcacc
     7621 ggtaaatacg gctttcaggt cgctgctgta gaacgaaagg cttcctgagg aatgccccgg
     7681 aacgtgaagt gcggtgagtc tgatctgtcc atgggaaaca atctgtccat cctccagata
     7741 atccgccggt tcgggaggta gttccatatt gagtccgaag agccgggcat gcaccggggc
     7801 atcgatcctg ttataatcat cctccctgca gtaatgcgaa cggataccga atcgttcaag
     7861 catccagccg ttcccgaaaa tgtgatcgag atggcagtgg gtattcagca gcaaaacggg
     7921 tttaagctta ttatcctgaa tgaaagaatc catttcatca aactcatgcc cgtagtagca
     7981 gccgcagtca ataattatac aatcccctga cggatcagca attatataag tatttacttc
     8041 aatcggatta aaaaccagtg tatatatctt cacttttcaa taatttttat tttcatattc
     8101 cggatggcat attaactgat tctgaaccat catgatccgc aaaatccccg tcgagctttt
     8161 tcatacaagg actcaggttt cccaaacaaa tcagcttttt acaattcctt ttatcatagg
     8221 cacaaaaatg aaatatccat gtgaggaata atcaaatttg ttttcttccg ttcgtacaac
     8281 caatgtcatg gtctgggaag ttgccgaacc aagcgggatc accagcctgc cccctatttt
     8341 aagctgacgc atcagctttt caggaacctc agcggcagcc gcagtgacta taatgccatc
     8401 aaaaggtccg tattggggtt gcccttcata cccgtcgccg aagaaaaagg ttgctgaata
     8461 gcccagcagt gacagagtat gctgggcctt cagatgcaat tccctgaacc tttcaattgt
     8521 ataaaccttt gcccccattt cagccaggat tgcggcctgg tatcccgatc cggtacctat
     8581 ctccagtatt ttatcccttt tcctaacctg cagaagcgag gtctgaaccg caacggtata
     8641 aggctgagaa attgtttgcc cggatgagat gggaaatgcc ttgtcggcat atgcatggtt
     8701 gaggaatgcc ggatccatga atgcatgacg gggtacggta ttaatggccc tcagtacttc
     8761 ctcatcggtg atccctttgc gccggagctc atcgacgagt tttttcctct tccccctggc
     8821 ttcaaaagag tctgtcatcc caaaaatcag atggtttgtt tccgtaaaga aaacaaaaaa
     8881 aatgattttt tcctgcaaag taatgtaagg aaggttaatt aactcccccg ggaaaaagat
     8941 tgacgccctg ctttcacagg gaagaattac ccgggatcaa tattctccga agaacacaga
     9001 agctgaacac caggatccaa cagactttaa cttagaggaa gcgtggccag cgggatatat
     9061 accggtccgg ccggtttaag gatactttca aaaaggacaa cttcatttat ccagactttc
     9121 tggaattcaa tattcctgta tttttccaga acccgtctga gattctcgat gtctttgatg
     9181 tttttcagcc tgcccagtgt caggtgtgga acaaagggtt tcccgtcaaa accgacatct
     9241 ccagcttcca aacactccgt gatcagggct ctcagtttaa taagcccatc ctgcggttta
     9301 attccggccc agatgattct cggatccctg aaatttctaa aaatcccggt tcctgccagc
     9361 atgaagtcga actttccgaa tccggcacat tttcctttga tcaactgagt tatgggagcg
     9421 atctttacga ccggggtatc ccccataaat gcaagggtaa cgtgtatgtt ccgaatatcg
     9481 gcccatttaa tattttcatt tccaagaagc gacctgaggt ttgcaaccag tcgttccgtt
     9541 tcggtggttg tttctatctt tacggcaata aatgttcgct taatcatatt cgacagatat
     9601 tattataatc tgcctgcttc gatctcctgc aggataattt ccataacagc atcctcaccg
     9661 tacgggtcat attcagcctt aagattggtc ccgaatattc tggcaatggc ctgccagaaa
     9721 gaggctgaca gatttcccct gtaaaacctt atcagttcat aggaggtgtt ccgtgtttcc
     9781 ctgtcgatga gcttgatgag aagtttgccc tgggtaatgg tcatattccg gacatcatcc
     9841 tcatattccc tgaaaacatt tttctcaacc tgccggagat attttttccg ttccttttca
     9901 tcgggaatat tcattaattc atgatttacc tgttccagtc ttgcccgtac aacaattcca
     9961 tacggataga tttttttcag gttataaatc agccgttcat acttgcggaa tggaaatttc
    10021 tttgacgttg actgtctgcc tatgataaca acttctttca ggtcaatttc aggaagactt
    10081 tcaccgtttc tttctttggt gtgcagcagg tagaagttac gcggcagtgt atcgttccct
    10141 gcaatagagc tatcggtctg ggccaaagtg atttccaggg aaaaagaaaa cagtaatatc
    10201 ggtatcacaa gtttcatgtg aatgtgatct cacgcaaagg tatcaaaata cgggccattt
    10261 gataaaacat gattttcgtt ccaaaattgc aggaaaaaac acaaccccct ttaattaccg
    10321 gataaataaa atggacgatt tcttctttcc cggacatata ccggtatatt gtatctaaaa
    10381 aagaacccct aatccaataa ttgccggagt atttatgaaa gtttttcata ttcatgaccg
    10441 gaataataac cacccggaaa acaatgtagg ttacgatggt tttatttccc ataattcctt
    10501 ttgcattact ttcccaaaaa gttaaatttg gaaaaagctt aattcatggc actgaatgtg
    10561 aatgatcttg aagaccagtc aagatacagg caattaatta aaatacctta tgatgaactc
    10621 attccattca tccttgatta tctgcgaagg aaatcgggcc tgattgtttt tttctggtcg
    10681 gcatgcatcc tcacacttgg tctggcatta acggtaaggg tcaacctcag tggctatttc
    10741 ccctataaat acctgttact tcactcaatc ctggggctgg ttgtgttccc gttat
//
LOCUS       DAOOYG010000088        10721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6624_length_10721_cov_5.936434, whole genome shotgun sequence.
ACCESSION   DAOOYG010000088 DAOOYG010000000
VERSION     DAOOYG010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6624_length_10721_cov_5.936434"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3716)
                     /locus_tag="PK288_08190"
     CDS             complement(<1..3716)
                     /locus_tag="PK288_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPM18944.1"
                     /translation="MNQRLPGKGRLYLGLDIGSVSLNTVLIDETCNVVENYYDYVQGK
                     PFFVLRERLSQIIRNLNSGEIAGTALTGTGGRLACGLIGGVFVNEIIAQATAADRLYP
                     ETQTIIEIGGEDSKLILLERDPSNGHSRLVDFEMNSVCAAGTGSFLDQQARRIGVPIE
                     KEFGEMALKSADPPRIAGRCSVFAKSDMIHHQQIATPLHDIVAGLCFALARNFRSNVA
                     RSREFKKPVLFAGGVAANTGMIRAFREILNLKDRELIIPEYHASMGAIGAVIYARENN
                     PEMNGFHGLKELDEYLAHDSSHFSSLPPLAESKAVYNTSISWRKNPGKKTKVYLGIDV
                     GSLSTNVVLIDRANRVVARRYLPTAGKPLEAIQRGLKEIYDEAGKDVEIMGAGTTGSG
                     RYLTGDFIGADTIQNEITAQAAAAIDYDPTVDTIFEIGGQDSKYISIEKGVVVDFEMN
                     KVCAAGTGSFLEEQAEKLNINIVNEFGCMALGSECPVKLGDRCTVFMESDLNTFMQRG
                     ARNDNLVGGLAYSIVYNYLQKVVGDKKVGNKIFFQGGVANNKAVVAAFEQVTGKKITI
                     PPHFDVTGAIGAAILARKSMKKGQKTAFRGFGIRNAEYEISRFACQGCTNHCEIRKVR
                     IAGEKKSLFYGGRCEKYETDSRKKIANDIPNLFQKRLEMLMDGYRETEAGTGGITIGI
                     PRALMVFFQQFPFWRTFFEQLGFTVVLSRESDKTLVARSLETITTETCLPVELIYGHV
                     IDLIEKGVDYVFLPFIVNAKFREGNKTFNCNCPWIQSYPFMVRAALRDKIDESKLLIP
                     TLHFRFFERALVKEMSSFFNTKFGIGKKTIKEAIYKADAVQVAFEKQLTGYGRNILKN
                     IPGNYRPVVLLGRPYNSTDPHLNLGLVEKLLARNVMPVPVDMLDLAPYNIFENYRNMY
                     WPNGQKIVAAAQLVAKTDNLHAVYISNFRCGPDSFIWHYVSEELKAKPFLHIEVDEHS
                     ADAGIITRIEAFLDSLKGSDLNSKGEITVFRPHADTLTSPAKRTLYFPYMHDGAYLIA
                     AAARSVGIPSEVLPKQTDEDISLGRKYTSSRECFPMICTTGSFLKKLFEPGADPSKMS
                     FFMPDHNGPCRFGQYNHFQRTLFDRLGFGEATILTPSNDTSYADLAGDKSQLFRINAW
                     KGFVAFDFLRKLYRENKPYEICKGDTKKLYEISLKKIERCIESGSKGLRRLLVEITSD
                     FMALNTDRSKRKPVVAILGEIYMRDNAGCNGNIA"
     gene            complement(4015..4923)
                     /locus_tag="PK288_08195"
     CDS             complement(4015..4923)
                     /locus_tag="PK288_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM18945.1"
                     /translation="MKRDIINIDEEKCNGCGLCVPNCHEGAIQIIDGKARLVSELMCD
                     GLGACIGYCPEGAITVETREAEPYNEIKVIEQMISKGKNTIVAHLKHLKDYGEEGFFN
                     EAYNYLRDNQAALDFSLAEVMDEVNSHGKEVLQGMHNNQRHSQNESAGGCPGSRSMIL
                     EKAPAIRETPGPEDQPSELRQWPVQMHLLNPNAPYLRGSDLLLAADCAAFSMGNFHSR
                     YLKGKSLAIACPKLDHGLDIYVEKLAAMAELAKINTITVLRMEVPCCGGLLQMTRAAM
                     SKASRKVPVKQIVVSLTGDVLSEEWV"
     gene            complement(5137..7107)
                     /locus_tag="PK288_08200"
     CDS             complement(5137..7107)
                     /locus_tag="PK288_08200"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HPM18946.1"
                     /translation="MDLRKAQINDDFITKLNGEWEFYWEKLLLPNDFLSKRADRDLFG
                     KVPAYWTSYKTGQVRMEPMGYATYRLTVLLPAGFRQPLGFNMPVFDSSYDLYVNGKYL
                     GGNGKPGKTENDTRPWYEKNFFRFDTDSDTLTILINVSNFSHRRGGFWLPVEMGSLEY
                     VRKHYSSSIARDWAVVSLLAGFSLFFLCFFIIYPRDRLILFFSLAVLGLALRPLFTSN
                     FLILSFVKPEWEWIVRIEYLLFYSVITAWYWFTVNLYPSKYFRSLAWFITVLLPLISI
                     FTLILPVKIFSYSVLLYYPALAVIIIYTLVGNVTGILRKNHTDYAYFAVFILLSYGVV
                     HDVIVSLGNPAGFHDYILPSILVLFVFTQAVLLLYKWVKALSETEKLQSELQYMNRNL
                     GIMVEERTQEINSRKEEIEKQNIKIALQNKQMSDTIQLKNKVFSVIAHDLRSPVVSIL
                     YMLNLLKEEEFKEKYDEFANLSIQYAQQVINLLENMLVWGKGQEDKIKYSPGRYDLAD
                     LILTNLSIFKEAADNKAISMNFTQIGNSKAYFDKDLLDIIIRNLLSNAIKYTPRGGRI
                     SILLKDRTISGEGTVLKVCDNGVGIPQVKQKYLFTATEIVSTPGTENEKGTGLGLKLC
                     QELIKLNKGTLSVESKEEEGTCFTVTLPEQEQ"
     gene            complement(7240..9315)
                     /locus_tag="PK288_08205"
     CDS             complement(7240..9315)
                     /locus_tag="PK288_08205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPM18947.1"
                     /translation="MIMISVSCKKTEKSDNLLLSEYDTPFGVPPFELIKASHFMPAFL
                     KGFEEQKLAVRKIIADTSEPDFVNTIKALEYSGELLTRVSRIFGSLNSAHTNDTLQAI
                     SREIAPLSARHRDDINLNDTLFRKVKTVYENRSKFRLTEEEKMVLEDTYRGFVRSGAA
                     LSQDDKAKLRKLNEELSLLSVQFGQNLLAETNAFRLVIDRHEDLAGLPDGIVEQAAAL
                     AKNMNLEGKWVFTIQYPIMEPFLKYAENRKLREKLFTAYFMKGDNDNERDNKSIIARI
                     ARLRLERSKILGYRTYADFALERSMAKTPERVMNFLGQVWDAAIPAAKAEVKAMQEII
                     NREGGQFKLAPWDWWYYAEKIKKEKYDLDDEITRPYFRIDNVISGMFYVANRLYNLTF
                     RVRNDIPKYHQDVVTYEVSRNGRHVGILMIDNYPRESKRGGAWCGALRGQSRDINGKM
                     ITPVVTMVTNSTPPGADKPSLLTADEANTLFHEFGHALHQLLSNSTYPSVSGTSVPRD
                     FVELPSQIMEHWVLEPEVLQVYAKHYQTGEVIPSEIVDKLERAGKFNTGFTTVEFLAA
                     ALLDMEYHTLTEPTDIDIRQFEKKAMDKYGLPDEIKPRYRSTYFNHIWSSGYAAGYYS
                     YYWSEMLDADAFLAFKETGDIFNRDVASRFEKEILSRGGTRDPSAMYIAFRGKEPGID
                     ALLKNRGLK"
     gene            complement(10475..>10721)
                     /locus_tag="PK288_08210"
     CDS             complement(10475..>10721)
                     /locus_tag="PK288_08210"
                     /inference="COORDINATES: protein motif:HMM:NF027525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="band-7 C-terminal domain-containing protein"
                     /protein_id="HPM18948.1"
                     /translation="ASEIEQVANATANGLRAIASAISEENGLNAVNLRIAEQYLTGFA
                     NLAKQNNTIILPANLSDVAGIVATATSMFNEVKDKKK"
BASE COUNT         2724 a         2481 c         2291 g         3225 t
ORIGIN      
        1 ttggctatat tgccgttaca gcctgcatta tctctcatgt agatctcccc gagaatggcc
       61 acaacaggtt ttcgtttgct cctgtcggta ttcaatgcca tgaaatcaga tgtaatttcc
      121 acaagaagac gccggagtcc tttggagcct gattcaatgc agcgttcaat cttttttagc
      181 gatatttcat aaagtttttt cgtatcgcct ttgcatatct catagggttt attttcacga
      241 tatagttttc tcaggaaatc gaatgccacg aagcctttcc acgcgtttat cctgaataac
      301 tggcttttat ccccggccag atcagcatat gatgtgtcat ttgaaggagt caatatggtt
      361 gcttcgccaa atcccagtct gtcaaacagt gtcctctgga aatgattata ttgcccgaac
      421 cggcaggggc cgttgtggtc aggcatgaag aagctcattt ttgaaggatc tgcacccggt
      481 tcgaaaagtt ttttcagaaa acttccggtt gtgcatatca tgggaaaaca ttctcttgat
      541 gatgtgtatt tcctgcctag tgagatatct tcatctgtct gtttaggcag aacttcggaa
      601 ggaatgccaa cgctacgggc tgccgctgcg ataaggtatg ccccatcgtg catgtatgga
      661 aaatacaggg tccgttttgc cggggaagta agtgtgtcag catggggcct gaagacagtg
      721 atctcacctt tgctattcag gtccgatcct ttcagactgt cgaggaatgc ctcaatccgc
      781 gtaattattc cggcatcagc cgagtgttca tcaacctcta tgtgcagaaa aggtttggct
      841 ttaagttcct cacttacgta atgccagatg aatgaatcag gtccgcatct gaaattactg
      901 atatatacgg catgcaggtt atccgttttt gccacaagct gtgctgcagc aactattttc
      961 tgaccattcg gccagtacat gttcctgtaa ttttcgaaaa tattgtatgg tgccagatcg
     1021 agcatgtcaa cggggacagg catcacattc ctggcaagga gcttttccac caggcccaga
     1081 ttcagatgcg gatcagtact gttgtaaggc cttccaagaa gtaccacggg cctgtagttt
     1141 ccggggatgt ttttcaggat atttcgtccg taccctgtca gttgtttttc aaatgccacc
     1201 tgtactgcat cagctttgta aatagcctct tttatggttt ttttaccaat gccgaactta
     1261 gtattgaaaa atgatgacat ttccttcaca agcgcccgtt cgaaaaaacg aaaatgcagt
     1321 gttgggatta ggagttttga ctcatcaatc ttatctctca gggcagccct gaccatgaat
     1381 ggataagact ggatccatgg gcagttgcag ttaaaggttt tgttgccttc cctaaacttt
     1441 gcattgacaa taaaaggcag gaatacataa tccacccctt tttctataag atcaataaca
     1501 tgtccgtaaa tcagttcaac cggcaggcat gtttcagttg ttattgtctc aagtgaccgg
     1561 gccacaagtg ttttatccga ttcccttgaa agaaccacgg tgaatccaag ttgttcaaag
     1621 aaagtgcgcc agaacgggaa ttgttggaag aagaccataa gggcacgcgg gataccgata
     1681 gtaataccgc cggttcctgc ttctgtttcc ctgtagccat ccatgagcat ttcgagcctt
     1741 ttctgaaaaa gattgggtat gtcgtttgca atcttttttc gactgtccgt ctcatacttt
     1801 tcacaacgtc ctccatagaa aagcgatttc ttttcccctg caatcctgac cttacgtatc
     1861 tcacaatgat ttgtacatcc ctgacatgca aacctgctga tttcgtattc ggcgtttctt
     1921 attccaaatc ccctgaacgc ggttttctga ccttttttca tcgatttccg ggccagtatt
     1981 gctgcaccta tggcgccggt gacatcgaag tgcggaggaa ttgtgatttt tttaccggtg
     2041 acctgctcaa aggcagctac aacagcctta ttgttagcaa cgcctccctg gaagaaaatt
     2101 ttattgccaa cctttttatc tccaactacc ttctgcagat agttatacac tattgaatag
     2161 gccaggccgc ctaccaggtt atcgtttctg gcccctcttt gcatgaaggt attcagatcc
     2221 gattccataa aaacggtgca tctgtcgcca agcttaaccg ggcattccga gcccaaggcc
     2281 atgcatccga attcattgac aatattaatg ttcagttttt cagcctgttc ctcaagaaat
     2341 gaaccggttc ccgccgcaca taccttattc atttcaaaat caacgacaac ccccttttca
     2401 atgctgatat acttagagtc ctggccgccg atctcaaata tcgtatcgac tgttggatca
     2461 tagtcaattg ctgcagctgc ctgggcagtt atttcattct ggatagtatc tgcaccgata
     2521 aaatctccgg tcaggtagcg gcccgatccg gtggttccgg ctcccattat ctcaacatcc
     2581 ttacccgcct catcatagat ctctttcaat cctctctgga tggcctcaag cggtttgcct
     2641 gccgtaggca ggtaccttct tgctactacc cggtttgccc tgtcaataag aacaacattt
     2701 gtgctgagcg aaccaacatc aatccctagg tacacttttg tcttttttcc cgggttcttt
     2761 cgccagctga tgcttgtgtt ataaacagcc tttgattctg ccaggggagg aaggctgctg
     2821 aaatgagatg aatcatgtgc aagatattca tcaagttctt tcagcccatg gaaaccattc
     2881 atctccggat tgttttcccg ggcgtaaata acagcgccta tggctcccat cgatgcatga
     2941 tattccggta ttatcagttc gcggtccttc aggttgagta tttccctgaa tgcccttatc
     3001 attcctgtat ttgctgccac gccaccggca aacagaacag gttttttaaa ttccctgctt
     3061 ctggcaacgt tgctccggaa attcctggca agtgcgaaac acaagccggc aacaatatcg
     3121 tgcagaggag tggcaatctg ctggtgatgg atcatgtcgc tcttggcaaa gacactgcat
     3181 ctcccggcga tccgtggcgg atcagccgat ttaagggcca tttcaccaaa ctccttttct
     3241 atgggcaccc cgattcgcct tgcctgctga tcaaggaatg atccggttcc tgcagcacag
     3301 acactgttca tctcgaaatc gaccagtctg gaatgaccgt tggaaggatc cctttcaagg
     3361 aggataagct tggaatcttc tcccccaatc tcaataatcg tttgagtctc aggatataat
     3421 cggtcagcag cagtagcctg ggcaattatt tcattaacaa agacaccgcc gatcagcccg
     3481 caagccagtc tgcctcccgt tccagtaagc gcagttccgg ctatttcccc tgagttaagg
     3541 tttcgtatga tctgggataa tctctccctg agaacaaaaa aaggcttacc ctgaacgtaa
     3601 tcatagtagt tctcaacgac attgcaggtt tcatcaataa gtacggtatt gagtgaaaca
     3661 gatcctatat cgagcccaag atacaacctg ccttttccgg gaagccgttg gttcattgcc
     3721 tgaaatgtaa ttttagtaat gaatgaagcc ggtagatccc aatgccgttc tactaagtta
     3781 gcaatttttt attcaaggac catgcagaca gtcatatttt tagcaagcct gcacggtaaa
     3841 atgtactggt cgggcaggat tgcctgtatc acattactgt cagctatccc tggcaactcc
     3901 cgaacgtgaa tttatatcac cggtggattg tccggtaata ccgggggaat taataccggg
     3961 ccagaagctg gcggggggta tcattactga attgtaccgt tcagtcaggc aggcttatac
     4021 ccattcctcg cttaaaacat ctccggtaag gctcacaact atctgcttta ccggaacttt
     4081 acgagatgct ttcgacatgg ctgccctggt catctgcaac aggcctccgc agcagggcac
     4141 ttccatcctt aggacggtaa ttgtattgat ttttgcaagt tctgccattg ctgcaagctt
     4201 ttccacataa atgtccagac cgtgatcgag ctttgggcag gcaatcgcga gggattttcc
     4261 tttcagatac ctgctgtgaa agttacccat tgaaaaagca gcacagtcgg ctgcaaggag
     4321 cagatcggac cctctgagat aaggtgcatt gggattgagc aggtgcatct ggaccggcca
     4381 ctgcctgagc tccgatggtt gatcttccgg accaggcgtt tccctgattg ccggagcttt
     4441 ttccaagatc atggaccggg accccggaca gcctcctgct gactcgttct gactgtgtct
     4501 ttgattatta tgcattccct gcagtacttc tttcccatgg ctgtttactt catccataac
     4561 ctcggcaaga ctgaaatcga gagcagcctg gttatccctg agataattgt atgcttcatt
     4621 gaagaaacct tcctcaccat aatctttaag atgtttcagg tgtgcaacaa tcgtgttttt
     4681 ccccttggat atcatttgtt caatgacctt tatttcatta taaggttcag cctcccttgt
     4741 ctcaacggtt atagctccct ccggacagta gccgatgcag gcgccaagtc cgtcgcacat
     4801 cagttcactt acaagccttg ccttgccgtc gattatctga atggcccctt catggcagtt
     4861 ggggacacac aaaccgcagc cattgcattt ttcttcgtca atatttatta tgtctctttt
     4921 catgtgaaaa taaattcata tactactgaa gattaagtga ttcacatgga acccacatga
     4981 attttatttc catgcttgtt tgtgattttg tatcatgtgg gtttcatatt ttctcttttc
     5041 cggtgcaaat tttaggaaaa taatgcctgt aattgcaaaa catgctacat atcgtaaatt
     5101 agcccggttc tggcagcagg atttcaatcc gtgcctttat tgctcctgtt cagggagggt
     5161 taccgtaaaa caggtgcctt cttcttcctt gctttccacc gacaatgtac ccttgttcaa
     5221 ttttataagc tcctggcaga gcttgagacc gaggccggtt cccttttcat tttcagtgcc
     5281 cggggtactt acgatctcag tggctgtaaa gagatatttc tgtttcacct gggggattcc
     5341 cacaccgttg tcacaaactt tcaatactgt accttctccc gatatggtcc tgtctttcag
     5401 gagaatgctg attcttccac ctctgggtgt gtattttatg gcatttgaca gaagatttct
     5461 gatgattata tcaagcagat ccttgtcgaa atatgccttt gaatttccta tctgggtaaa
     5521 gttcattgag atcgccttat tgtctgctgc ttccttgaaa atgctcaggt tggtgagaat
     5581 aaggtcggca aggtcgtagc gtccgggtga gtattttatt ttatcttcct gaccttttcc
     5641 ccatacaagc atgttttcaa gaagattaat gacctgctgg gcatactgga tactcagatt
     5701 tgcaaattca tcatattttt ccttgaactc ttcttctttc agcaggttaa gcatgtacaa
     5761 tatacttacc acggggctgc ggagatcgtg ggcaatgacc gaaaagacct tgttcttaag
     5821 ttggatcgta tctgacatct gcttgttctg cagagcgatc tttatatttt gcttttcaat
     5881 ctcttcttta cggctgttta tttcctgtgt tctttcctcc accatgatcc cgagattcct
     5941 gttcatgtat tggagttcgc tttgcagctt ttctgtttcg gaaagggctt taacccattt
     6001 gtatagaagc aaaactgcct gggtgaaaac aaataaaacg agtattgatg gcaggatata
     6061 gtcatggaac ccggccggat tgcccaacga tacgatgaca tcatgaacaa ctccgtatga
     6121 gagcaggata aaaactgcaa aataggcata gtcggtatga ttttttctta atatgccggt
     6181 cacgttcccg accagggtat aaataataat taccgccagg gccggataat aaagaagtac
     6241 gctgtaactg aatattttaa ccgggaggat taaagtgaag attgatatta gcggcaataa
     6301 aacggtaatg aaccaggcaa gactcctgaa gtattttgac ggatacagat ttacggtgaa
     6361 ccagtaccag gctgttataa cggaatagaa caacaagtac tcaatcctta cgatccattc
     6421 ccattcaggt tttacaaacg aaagaatcag gaagtttgaa gtaaacagcg gtctcagtgc
     6481 caggcccaat acagccagac tgaaaaataa tatcagcctg tcccttggat aaattatgaa
     6541 aaagcaaagg aaaaaaaggg aaaatcctgc cagaaggctg acaaccgccc agtctctggc
     6601 aatggaagat gaataatgtt tccggacata ttccaggctg cccatttcaa ccggaagcca
     6661 gaaaccaccc cgccggtggc tgaaattcga cacatttatt aaaatcgtaa gtgtatctga
     6721 gtcagtgtca aacctgaaga aattcttttc ataccacggc cttgtgtcat tttcagtttt
     6781 tccgggttta ccgtttcctc caagatattt accgttaaca taaagatcat atgatgagtc
     6841 aaataccggc atattgaatc ccagaggctg tctgaatcca gcgggcagaa gaaccgtaag
     6901 cctgtaagtg gcatatccca ttggttccat ccgtacctgg ccggttttgt aactggtcca
     6961 gtaggctggt acttttccga aaagatcgcg gtctgccctt tttgaaagaa agtcgttcgg
     7021 aagaagcagc ttttcccagt aaaactccca ttccccgttg agtttggtta tgaagtcgtc
     7081 attaatttga gccttacgaa ggtccatgac accttccgca gcaaatcccg gacgttcgga
     7141 agaacgacag ccgcacaatg ataataatgc gaatgataga attataccgg ttattttcat
     7201 ttatttgtta gcgaaaatct atcaaaggcg gtttcaactt tatttcaatc ccctgttttt
     7261 cagcagggca tctattccag gttcttttcc cctgaaggct atatacatag cagatggatc
     7321 gcgagttcct cctcttgaga gtatttcctt ttcaaatctt gatgccacat ccctgttgaa
     7381 aatgtcacct gtttccttga aagcgaggaa ggcgtcggcg tcaagcattt cgctccaata
     7441 gtagctgtaa tatcctgcag cataccccga actccagatg tgattaaagt aggttgacct
     7501 gtatcttggc ttgatctcat caggcaatcc gtatttatcc atggcttttt tttcgaattg
     7561 cctgatatcg atgtcggttg gttccgttag agtgtgatac tccatgtcga gaagagcagc
     7621 agcaagaaac tcgacagttg taaatcccgt gttgaatttg cctgcccttt cgagtttatc
     7681 tacaatctct gaagggataa cttcgccagt ctgatagtgt tttgcatata cctgtagtac
     7741 ctcgggttcc agaacccagt gttccataat ctgggaagga agttctacaa aatcgcgtgg
     7801 cactgaagtg cccgaaacac tcgggtaggt ggaattcgaa agaagctggt gcaatgcatg
     7861 tccgaattca tggaacaggg tattggcttc atccgccgta agaagtgaag gtttgtcagc
     7921 tccggggggt gtggaattgg tgaccattgt gacaacaggt gttatcattt taccgtttat
     7981 gtcacgactc tggcctctaa gggctccgca ccatgcccct cctcttttgg attccctggg
     8041 gtagttgtca atcatcagaa tgcctacatg cctgccgttc ctgcttactt cataggttac
     8101 gacatcctga tggtatttcg ggatatcatt gcgcaccctg aatgtgagat tgtaaagcct
     8161 gtttgcaaca taaaacattc cgctgataac attgtcaatc ctgaaatatg gtcgtgttat
     8221 ttcgtcatcc aggtcatact tctccttttt tatcttttcg gcatagtacc accagtccca
     8281 tggcgccagt ttgaactggc caccttccct gttgatgatc tcctgcatgg ccttaacttc
     8341 agcctttgcc gcaggtattg cagcgtccca tacctgacca agaaaattca tgaccctttc
     8401 aggagttttg gccatgctcc tttccagtgc aaaatcggca tatgttctgt atcccaggat
     8461 ctttgacctt tcaagcctga gccttgctat ccgggcgatg attgatttgt tgtccctttc
     8521 gttgtcatta tcacctttca taaagtaggc tgtaaacagc ttttccctca gtttccggtt
     8581 ttcagcatac ttcaggaacg gctccatgat cggatactgg atggtaaaaa cccatttccc
     8641 ttcaagattc atgttttttg ccagtgctgc tgcctgttca acaataccgt caggcagccc
     8701 tgcaagatcc tcatgcctgt caataacaag cctgaatgca tttgtctcgg caaggagatt
     8761 ctggccaaac tgaacagaca gaagtgacag ttcctcattt agcttccgca gttttgcctt
     8821 gtcatcctgc gataatgctg cgccgctcct cacaaatccc ctgtatgtat cttcaagtac
     8881 cattttttct tcctcagtga gtctgaactt cgaacggttc tcataaacag ttttaacctt
     8941 ccggaaaaga gtgtcgttga gattaatatc gtccctgtgc ctggctgaaa ggggagctat
     9001 ttccctgctg attgcctgca aggtatcatt agtgtgggca gaattaagtg atccgaaaat
     9061 cctgctcacc ctcgtcagca gctctccgct gtattcaagt gcttttatgg tatttacgaa
     9121 atcaggctca ctcgtatcgg caattatttt tctgacagcc agtttctgct cttcgaatcc
     9181 tttcaggaat gcaggcatga aatgcgaagc cttaatgagt tcaaaaggcg gcaccccaaa
     9241 cggagtatca tattcactta acaaaaggtt gtcagatttc tccgtttttt tgcatgatac
     9301 agaaatcata atcatgaatc cgattactaa tatggctttg ttcatcataa aataattttg
     9361 atgtaaatga tatgatgcct gcctcacacc tcgggcaggg aacattagtg ttgaaattaa
     9421 tatttattgt gattatgtat tgcatgcatg tggattttat cctgaaatgg tttgaacaag
     9481 tgaaagtaag aagttaaaaa caaacgacaa aggataatat tcagaagaca aaagtacaaa
     9541 gaaacacaga actggaaaca ggaaaaccaa atcagtccgt aacattggaa gggattcatg
     9601 taatctcata ctcagatgag gtattgaaac ggatgaacaa atataaaaaa gaaaggaaag
     9661 atagttggga gttatctttc cttcattaac ctaaacctga tcgtagaatt tggccgaacg
     9721 gcctataaag aaatcttata tatttttatg ttatttttcc ttccggaaaa taatcatgta
     9781 atcaaagaac acccttttta ttcccattta caccatatag acgtataaga ctgccttttt
     9841 gttgcataaa ctattgttaa tgttatgtta atttttgcaa tcagttttcc acggaaagag
     9901 aaattttcgg atagtcggga tattgtgctg tattacagaa gattatgatt tcaggcctga
     9961 aataccggtt catgatgaat atgtcggagt gccggagtta tttctgtctt atctatctta
    10021 cccggtaatt tccggggaat aattcatttt cccgttatac atttcttttt attacctatt
    10081 tattcctgga aagctcttat tttccttgca gggcctgttt tccctttcgg catatatcat
    10141 ataggcaccc agggcaatat accggggtgt ccggttcggt gatcccgggc gatctgaagt
    10201 tgatcctcat ggacggaatg aatacaatta tgcatatgcc tgtgattctg tggcggtact
    10261 aaaatccggc aaattccttt ttgctgcggg agttgctgaa acagcttaag ttccggaatc
    10321 tctggaaatt caaccttctt ctttaacaat gaaacaaccc cgcggacagg gcatggaagt
    10381 gcatcagccc ggggtttttt tgattcgtca ggctcagaag tcagaccact cctacgggta
    10441 ggaggggtcg gaatcaggtc atcgggatta cagcttattt ctttttatcc ttaacctcat
    10501 tgaacatgga agtagcagtt gcaacgatac cggccacgtc tgaaagattt gccgggagga
    10561 ttatggtatt gttctgtttt gccaggttag cgaagccagt caggtattgt tctgcaatac
    10621 gcaggttgac tgcattgagt ccgttttctt cagaaatggc cgatgcaatg gctctcaagc
    10681 cgttggctgt tgcatttgcc acctgttcaa tttctgatgc a
//
LOCUS       DAOOYG010000089        10608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6720_length_10608_cov_5.309201, whole genome shotgun sequence.
ACCESSION   DAOOYG010000089 DAOOYG010000000
VERSION     DAOOYG010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6720_length_10608_cov_5.309201"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3146)
                     /locus_tag="PK288_08215"
     CDS             complement(<1..3146)
                     /locus_tag="PK288_08215"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPM18949.1"
                     /translation="MKLIRIFSSIICLAILSSWLYSQDRRTTATKVADILAQFPARDN
                     TATDKLMTDLLSLGEEGLRMVCDQIIPAGTGDDTRARFAIESLSRYLSQRENGDQKSS
                     WEKICISYVNSRRSSDVRDFFMKQLQLVGSDASVEAVRDLLTDRALCAPALGVLESVG
                     GKLAESVLAGSLSDKDLPCADAVMNVLAEMGSDIAVNEYIARVNDPDPRIRAAALNAL
                     ASSGSPLAMPVLSKAAKDILYRWEPTGASEALLNYAEKVGSKGDLKTMDKICKLFMAK
                     ANDKLSVQNSSRALAIYAALHGTDAMKEIKKAASSPDKSYRNAAFRASLALQGKEITK
                     QWTDYFPKAIPAAKPEIIAMLGERRDETALQLVTASLSSGDKGLRTEAAHALAMISGS
                     KAVPAIIEYLRKYPDAEDQQAAREALIFISGDKETGMLVPVVREGPEYARATAIGLLA
                     RSKVNEYFSLVRSFTSSDDENVRSAAYKALADLAGPDDQLTLIDLIRKTANSDYIADV
                     QAALASAAAKIPDPEKRSDVILKAMGNNEDKEKLIPVLAKTGGRTALKTVLHEFENGN
                     AGMRDICFATLTSWKDYTASSALYEICSSGNKTFEEPAFRAYVSQIRGADLNDDQKLL
                     AYRKIMPYALSAERKNSILNQVAALKTYQALFFAAGFLDDPSTSPAAAGAVMTIALPP
                     AGEESGMYGRLVREILEKASGKLTGPESDYEREMIAKYLSSMPSDEGFKPMFNGKDLT
                     GWQGLVENPIVRSKMKKADLARRQAEANTLIPASWSVKDGCIWFNGTGDNLCSVDDYG
                     DFELLVDWKITKGGDSGIYLRGSPQVQIWDTSRTNVGAQVGSGGLYNNQVNASKPLMV
                     ADNPVGDWNSFRILMIGENVSVWLNGHLVVDNVVLENYWDRRIPIFPRGPIELQAHGT
                     DLAFRDIYVREITDKDYNLTPEEKAEGFVSLFNGRNLDGWVGNKQSYVAEDGMIVIKP
                     ESGSGGNLYTEREYSDFIFRFEFQLTPAANNGLGIRAPLEGDAAYAGMELQILDNTAE
                     VYSKLQPYQYHGSVYGVIP"
     gene            complement(3187..4455)
                     /locus_tag="PK288_08220"
     CDS             complement(3187..4455)
                     /locus_tag="PK288_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18950.1"
                     /translation="MKQNGSDRRSFLKKSTAALFAFTIVPRHILGGSGYTAPSDQLTK
                     GIVGVGGMGRGHIEYEGTRLLAVCDVDKNHLAMAIAQAGGNVDGYHDFRELIMRPDID
                     IVHIATPPHWHGIIAKMAAEAGKDIWCEKPMTRTIGEGIKLVKAVQEHGRMFRLNTWF
                     RFKDQFYGLGTTVKPLKKLIDSGVLGWPLKVTVSGITGFDWKFYWSGLHNLTPMPVPP
                     ELDYDFWLGPAPYKHYNPERVHSKFRGYWDYDGGGLGDMGQHYLDPVQYILGKDDTGP
                     VSVEIDAPQQHYDAVGSWRRITYTYADGCKIILDGENRDKDAAYVEGPHGKIYRGFRT
                     DIRDVQRLIDSLPDPEPQIMTFSESVRTRKKFALNEANGHRSCSIVNLGIVALRLGRN
                     LKYDPGKQEFIDDEGANRLIDQPMRGEWKM"
     gene            complement(4699..5853)
                     /locus_tag="PK288_08225"
     CDS             complement(4699..5853)
                     /locus_tag="PK288_08225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM18951.1"
                     /translation="MATREINISKILPVLMAFFVMSFVDLVGIGVDRVSKDMSLSATV
                     AQLIPSAAFLWFFILSVPVGVLQSRLGKKTMLNIGMGVTALGLLVPFFMYSFAMVLFG
                     FALLGIGNTIVQVSANPLLVDVVPGNRASSFLSFSQFIKAVGSMLGAPLAGFFAAKFG
                     DWKLLFLVFGIVSILAVLWLGTSKITETRVEGYKATFGSSFKLLGNNFVLLMVLSIFV
                     VVGVDVGFNSNSGQFLIKQFGIEQTAAESGRSVYFFGRMLGTFAGAIMLTRISSRSFF
                     MWTSILGILCLAAILLVKSGAVAWILIFFIGLAIANIWPLVFSIAVEKHPLQNNEISG
                     LMMMAISGGAVIPLLIGWISDMSNISWGMSVLIICMIYLLAVSIYCLKTR"
     gene            complement(5952..6887)
                     /locus_tag="PK288_08230"
     CDS             complement(5952..6887)
                     /locus_tag="PK288_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPM18952.1"
                     /translation="MENEIAIGADIGGSHISCAAVGLNDLRIFRDTLTERAVDNKASA
                     DEILNIWAEALSDSIKKVPGQLIKGIGFGMPGPFDYVNGVCYIKGVAKYENLYGWNVK
                     DAISERLKSFGEFPVRFMNDVSAFAVGEALVGKASSAGRSMAITLGTGFGSAFIDNRI
                     PICDGPEVPELGCVYHLPYGDNIADDYFSTRWFLGSYKKLTGRTLSGVKEFAEIAATD
                     KTAMNLFTEFANNLGKFLAPWLIKFKAEIIVVGGNISNAYNYFGKTFEDSLAREGCTC
                     KVALSELKEDAAFIGSAFLLDDKFWAGIQHALPLM"
     gene            7119..8360
                     /locus_tag="PK288_08235"
                     /pseudo
     CDS             7119..8360
                     /locus_tag="PK288_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017113.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            complement(8423..9145)
                     /locus_tag="PK288_08240"
     CDS             complement(8423..9145)
                     /locus_tag="PK288_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPM18953.1"
                     /translation="MEKFFDYFDERYRKSVLKAPSENDGPVITISRLTGCDAREVASK
                     VVEKLNEKFGTEKWRWVDKDIIYSIAKELNIDTQRIENYYEGFKLSDLSEMIMAFSGG
                     FVSDLRVKKAIRDVVLSICKEGYIVIVGRGGVSIARDIKDALHVRLIAPFYWRVNNIM
                     RKKNMGIEEAEKFIVETDEKRADLIQTFLDKKIKNTDSLFDVTLNRLSFGIDDSASLI
                     TTMYEKKVHKQITERLKQRSFI"
     gene            9418..9882
                     /locus_tag="PK288_08245"
     CDS             9418..9882
                     /locus_tag="PK288_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPM18954.1"
                     /translation="MDSPLLSRPEIIWFIIGLALFLLELVLPGFVIFFFGVGAWVTAL
                     LCLIANPGLNLQVIVFAVTSVLSLLALRRMIQKRFFLARNDGLSDSVEDEFTGREAVA
                     VGSFKPGETGKVDFKGTTWNAESEFYITDGQRVIIINKESFKLFVEPKNNQK"
     gene            9879..>10608
                     /locus_tag="PK288_08250"
     CDS             9879..>10608
                     /locus_tag="PK288_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HPM18955.1"
                     /translation="MTAAIALQTASSTTFILIGIILLAFILISSMVKVVPQRTAMIVE
                     RLGKYRATFTAGFQVLIPFVDKIRYRHTLKEQAIDVASQTCITKDNIAIEVDGILYLQ
                     VLDPQKASYGIDNYRFASIQIAQTTMRSVIGKLELDRTFEERESINATIVEAVDKASE
                     PWGVKVTRYEVKNIIPPQSIKDAMEKQMRAEREKRALIAESEGTRQAKINVADGDKQE
                     FILRSEGEKQKRINEAAGRASEIEQ"
BASE COUNT         2808 a         2650 c         2265 g         2885 t
ORIGIN      
        1 gggatcacgc cgtaaaccga accatggtac tgatagggct gcaattttga atagacttcg
       61 gcagtattgt caaggatctg cagctccatt ccggcatagg ccgcatcacc ctccagcggg
      121 gcacggattc ccagtccgtt attcgctgca ggtgttagct ggaactcaaa tctgaaaata
      181 aaatccgaat attccctttc cgtataaaga ttccctccgc tgcccgattc cggcttgatc
      241 acgatcatcc cgtcttcggc aacatatgat tgcttgttcc ccacccagcc gtcgagattc
      301 ctcccgttaa acagtgaaac aaatccttca gctttttctt caggtgtgag attgtagtcc
      361 ttatcagtga tctccctgac atatatatcc ctgaaggcca ggtcggtacc atgagcctgg
      421 agttcaatcg gccccctggg gaagataggt atcctgcggt cccagtagtt ttccaggaca
      481 acattgtcaa cgacaaggtg accattgagc catacagaaa cattttcccc gatcattagt
      541 attctgaagc tgttccagtc tcccacggga ttatctgcaa ccataagcgg tttagaagca
      601 ttcacctggt tgttgtacaa tccgcccgat cccacctgtg ccccgacatt ggtccgggat
      661 gtatcccata tctgaacctg tggcgatccg cggaggtaaa tgccgctgtc tcctcccttg
      721 gtaatcttcc agtcaacaag caattcaaaa tcaccataat catcaaccga gcaaagatta
      781 tcgcctgttc cgttgaacca gatgcagcca tccttcacgc tccagctggc tggtatgagg
      841 gtatttgctt cagcctgtct gcgggcaaga tccgcctttt tcattttcga cctcacgatc
      901 ggattttcaa caagcccctg ccagcccgtc agatcctttc cgttgaacat cggtttgaat
      961 ccttcatcag acggcattga ggaaagatat ttggcaatca tttccctctc atagtcactt
     1021 tccggaccag ttagctttcc tgaagctttt tcaagaattt ccctcaccag tcggccatac
     1081 ataccgctct cttctcctgc agggggaagt gcaatagtca taactgcacc ggcagcggca
     1141 ggggaggtgg acggatcatc aaggaagcct gcggcaaaga aaagagcctg gtaggttttc
     1201 agtgctgcca cctgattcag tatactgttc tttctttcag cactcagggc atagggcatg
     1261 attttcctgt aagcaagaag cttctgatcg tcgttaaggt ctgcacccct tatctgggat
     1321 acatatgccc tgaaagctgg ctcctcaaag gtcttatttc ctgatgaaca aatttcataa
     1381 agggccgatg aagcagtgta atccttccat gatgtcaggg ttgcaaagca gatatccctc
     1441 attccggcgt tcccgttttc aaactcatgc aaaaccgtct tcagggcagt tcttcctccg
     1501 gtctttgcca gtacgggaat cagcttttcc ttgtcttcat tgttccccat tgctttgagg
     1561 atgacatcag acctcttttc aggatcaggg atctttgctg cagctgatgc aagagctgcc
     1621 tgaacatccg caatataatc gctgtttgcg gttttcctga taaggtctat aagggtcagc
     1681 tgatcatccg ggccggcaag gtcggccagc gccttatatg cagcagatct gacgttttca
     1741 tcatctgagg aggtaaaaga cctgaccaga ctgaaatatt catttacttt actccgtgca
     1801 agcagtccga ttgcagttgc ccttgcatat tcaggtcctt ccctgacgac gggcacaagc
     1861 attcctgttt ccttgtcgcc cgatatgaaa atcagtgcct cccttgctgc ttgctggtct
     1921 tcagcatcag ggtacttcct gagatattca attatcgccg gcactgcctt gctgccgctg
     1981 atcatggcca gggcatgcgc ggcctcagtt ctcagtccct tgtcacctga tgagagtgaa
     2041 gctgtaacaa gctgaagtgc agtttcatcc ctcctctctc ccagcatggc aatgatctcg
     2101 ggttttgctg cgggaatggc tttcgggaaa taatctgtcc attgttttgt gatttctttt
     2161 ccctgtagcg caagcgaagc cctgaaggca gcattcctgt atgatttatc cggactggaa
     2221 gctgcttttt taatctcctt cattgcatct gtcccgtgca acgcggcata tattgcaagg
     2281 gcgcgcgaac tgttttgaac cgaaagctta tcattggcct tcgccataaa gagcttacag
     2341 attttatcca tggttttcag atcgcctttt gacccgactt tctccgcata gttcagcaat
     2401 gcttccgatg cccctgtagg ttcccatctg taaagtatgt cttttgcagc tttcgacagt
     2461 accggcatgg ccagcgggct cccgctgctt gcaagtgcat tcagggcagc tgccctgatc
     2521 ctgggatcag gatcatttac gcgtgctata tactcattta ctgcaatgtc cgaacccatt
     2581 tccgccagta cattcatcac agcatcggca cagggaagat ccttgtcaga aagagatccg
     2641 gcaagaacgg attctgccag cttccctccc acggattcca atactccaag ggcaggagca
     2701 cacagtgccc tgtcagtcag caaatccctg acagcctcca cagaagcatc gcttccgaca
     2761 agctggagct gcttcatgaa aaaatccctg acatcactgc tcctccgact gttaacataa
     2821 gatatgcata ttttctccca tgaagatttc tgatcaccat tttctctttg cgacaggtaa
     2881 cgcgacagac tttctatggc aaatcttgcc cgggtatcat cgcctgtacc cgccggaatg
     2941 atctggtcgc aaaccattct gagtccttct tcacctagcg aaagcagatc tgtcatcaat
     3001 ttatcggtgg cagtgttgtc gcgggcaggg aactgcgcaa gaatatccgc gaccttggtt
     3061 gcggtcgttc tcctgtcctg ggaataaagc cacgatgaca ggattgctaa acatattatg
     3121 gatgaaaaaa ttcggattaa tttcattttt ttattttttt cggtaattca attcatcagt
     3181 tcctggtcac attttccatt ctcctctcat gggttggtct atcagcctgt tcgcaccttc
     3241 atcatcaatg aattcctgtt tcccaggatc atatttcagg tttctcccaa gccgcaatgc
     3301 cactattcca agatttacta tcgaacacga cctgtgtccg ttggcttcgt taagagcaaa
     3361 cttcttcctt gttctgacag attcggaaaa tgtcattatc tggggttcag gatcgggaag
     3421 ggaatcaatc agtctttgaa catccctgat atcggttctg aagcccctgt aaatttttcc
     3481 atgcggccct tcaacataag ctgcgtcctt gtcacggttc tctccatcca gtattatttt
     3541 gcatccgtcg gcataggtat aggttatcct tcgccatgac cccactgcgt cgtaatgctg
     3601 ttgcggagcg tctatttcaa ccgaaaccgg ccctgtatca tcctttccca gaatgtattg
     3661 tacaggatca agataatgct gtcccatatc tcccaggccg cctccgtcat agtcccagta
     3721 tcccctgaat ttgctgtgca ccctttcggg attgtaatgc ttatatggtg cggggccgag
     3781 ccagaaatca taatcaagct cggggggaac aggcatcggc gtgagattgt gcaggccgct
     3841 ccagtagaac ttccagtcaa acccggtgat accgctgacc gtcaccttga gcggccaccc
     3901 aagcacaccg ctgtcaataa gctttttcag cggttttacg gttgttccca aaccatagaa
     3961 ctgatccttg aacctgaacc atgtattaag acggaacatc cttccatgtt cctgaaccgc
     4021 cttcaccagc tttatcccct cgcctatcgt cctggtcatc ggtttttcac accatatatc
     4081 ctttccggct tctgcagcca ttttggctat aatgccatgc cagtgcggag gtgtcgctat
     4141 atgcactata tcaatatcag ggcgcataat cagttccctg aaatcgtggt acccgtctac
     4201 attgccccct gcctgtgcta ttgccatggc caggtgattc ttgtctacat cacagactgc
     4261 gagaagccgg gttccttcat attcaatatg tccccggccc atgccgccaa ctcccacaat
     4321 ccccttggta agctggtcgg aaggcgccgt gtaacccgac ccgccaagaa tatgccttgg
     4381 aactatggta aaagcaaaaa gggcagccgt actcttctta aggaaactac gcctgtcaga
     4441 tccattctgt ttcatcataa tataattttg atttcaattg ttttccttca gtccttcata
     4501 aaaggtaaca ctacacatgc ttccaaaata cggttcgcaa tgcactgata ttaaaaacca
     4561 gatcatcagg gacctgtctt ctgtttttca ccctgttcag gatttcccat cctgaaacct
     4621 cttaatgtaa tattgcttcc gaaccaaaaa acatggctgg aacccgtgat gtcccggtcc
     4681 ctgcaaattc gctggctatc accttgtttt caggcaatat atggaaaccg ccaggaggta
     4741 tatcatacaa ataataagca ccgacattcc ccatgaaata ttgctcatat cactgatcca
     4801 tcctatcaga agcgggatga ctgctccgcc tgagattgcc atcatcatga gtcccgatat
     4861 ttcattattc tgcaggggat gtttctcaac agctattgaa aagacaagtg gccatatatt
     4921 ggctattgca aggccgatga agaatatcag tatccatgca acagccccgg atttgacaag
     4981 cagaatggcc gccaggcaaa ggatcccgag tatacttgtc cacataaaaa agctcctgga
     5041 agatattctt gtcagcataa tggctcctgc aaacgtgccc agcatacggc caaagaaata
     5101 gacactgcgt cccgattcgg cagcagtttg ttcaatcccg aactgtttga tcagaaactg
     5161 cccggagttt gaattgaatc ccacatccac accaactact acaaaaatgg agagcaccat
     5221 cagcaatacg aaattatttc caaggagttt aaaggatgaa ccgaaagttg ccttgtaacc
     5281 ttcaaccctg gtctcggtga tcttggatgt tccaagccac agtacggcaa ggatggaaac
     5341 tatcccgaac accaggaaca gaagtttcca gtcaccgaat ttcgcggcaa aaaagccggc
     5401 aagcggagct cccagcatcg atcctactgc tttgataaac tgggagaagc tcaggaagct
     5461 ggatgccctg tttcccggca caacatcaac aagcaacggg ttggccgata cctgtacaat
     5521 tgtgtttcct atacccagca atgcgaagcc gaaaaggacc atggcaaatg aatacataaa
     5581 aaacggaacc agcaggccca gggctgtcac gcccatgcct atgttcagca ttgtcttttt
     5641 cccgagccgc gattgaagta cccccacagg cacggaaagt ataaaaaacc agaggaaggc
     5701 tgcggatggg ataagctgtg ccacagtggc actgagactc atatccttgc tcaccctgtc
     5761 gaccccgatt ccaacgagat cgacaaagct cataacgaaa aatgccatca aaacaggcaa
     5821 tatttttgaa atgttgattt cgcgggtagc cataggtagt tgattttagg gtttattttt
     5881 tctgctgcgt tccgacaccg gtgatttctg acaacatgat gccggtgaca gccttcagac
     5941 agcagtgttt tttacatcag tggaagagca tgctgaatgc ctgcccagaa tttatcgtca
     6001 agaaggaaag cgcttccgat gaaggcggca tcctctttca attccgacaa ggcaaccttg
     6061 caggtgcatc cttctctcgc aaggctgtct tcaaatgtct ttccaaaata attgtatgca
     6121 ttcgatatat tgcccccgac gacaattatt tccgccttga acttaataag ccagggcgcc
     6181 agaaatttgc ccaggttgtt cgcaaattct gtgaacaggt tcattgcggt cttgtcggtt
     6241 gccgcaattt cagcaaattc cttgaccccg cttagcgtcc tgcccgtaag ctttttatat
     6301 gaccccagga accatcgtgt tgaaaaatag tcgtcagcta tgttgtcacc ataaggaagg
     6361 tgataaacgc atcccagttc agggacctcg gggccgtcgc atatcgggat cctgttgtcg
     6421 ataaaggctg aaccaaaacc cgttcccagg gttattgcca tcgaccgtcc tgcagaagag
     6481 gctttgccca caagtgcctc accaacggca aaagcggaaa catcattcat aaaccttacc
     6541 ggaaattccc caaatgactt cagcctttca gagatagcat cctttacatt ccacccgtaa
     6601 agattttcat acttggccac accctttata tagcataccc catttacata gtcaaaaggg
     6661 ccgggcatcc caaatccaat accttttatt aactgacccg ggaccttttt tatcgagtcc
     6721 gacagggctt cggcccagat attcaggatt tcgtcggcag aagctttgtt gtcgactgcc
     6781 ctttcggtca gggtatccct gaatatcctg agatcgttaa gccccacagc tgcacagctt
     6841 atatgactgc cgccaatgtc ggccccgatg gcaatttcat tttccatata tcttatttta
     6901 gaatgggata ataattttag atgctgaaaa tattgaattt tgtcgtgaat acaaaaaaat
     6961 aatatttaaa aatacctgct cacatcttat gtgatctttc tcatttttat tttcaccgat
     7021 ttattcgtac tttgatatac agtatctttc tgttcacggg tttctgattg agtacaaaca
     7081 cttccgggaa ttttaaaaac cagtaaaaac agtaccaaat gccaaaagta acaagggaag
     7141 acgctttgtt ctatcatagc agggggcgtc acggaaaagt ggaagtaata cccacaaaac
     7201 attacgatac acagctcgat ctcagcctgg cttacacgcc gggagtggcc tatccgtgtc
     7261 ttgagattgc aaaggatcct gaaaatacct ataactacac cgctaaggga aaccttgttg
     7321 cagtcatatc aaacggaaca gcggtccttg gccttggaga tatcggggct ctggcgggaa
     7381 agccggtaat ggaaggcaag gggcttcttt ttaaggtttt tgctgatatt gacgtttttg
     7441 atattgaagt aaatgaaaaa gatcccgaaa agcttgttga aatatgtgaa aaaatctcgc
     7501 ctacattcgg aggaatcaat ctcgaagata ttaaagcacc agactgtttt gaagtggagt
     7561 cgcggcttaa aagcctggtg gacattcctg tttttcatga tgatcagcat ggcacggcaa
     7621 tcatttccgc tgccggcctg attaattccc ttgaattaac cggccggaaa gcagaagacc
     7681 tgaaaatggt tgtctgcggt gccggagccg ccgctatctc ctgttccagg ctttacctgg
     7741 aactgggggt gaaaagggaa aatatcgtga tggctgacag caagggtata ttaaacagga
     7801 aaagaagaga cctgaacaaa tacaaacagg aattcgttac ggatcgggat gtaaatacac
     7861 tccaggaagc attgaaagga gctgacatgt tcctgggtct gtccaccgcc aatatactta
     7921 cacgggaaat gcttgtttcc atggctgaaa atcccattgt ttttgcaatg gccaatccaa
     7981 atcccgagat cgcttatgag gaggctatgg ctgccagaaa tgatctgatc tttgctaccg
     8041 gcagatcaga ttatccgaac cagataaaca atgtgctcgg atttcccttc attttcaggg
     8101 gtgcacttga tgtaagagcc tcaaccataa acgaggaaat gaaaattgca gcagcaaaag
     8161 cccttgcatg cctggccaag gaacctgtgc cggaatctgt tttacgggct tacaatcttg
     8221 aatcactttc gttcgggagg aattatatca taccaaaacc cctcgaccac cggcttattt
     8281 catgtgttgc ctcatgcgtt gcaaaggctg caatggatac gggagttgcc agggtgccgg
     8341 taaccgactg ggaggtatac aaaacaggcc tggaaaagag aatccatgag catataaaga
     8401 aagtgggaga aataaacgag tattagataa aggaacgttg tttaagcctt tcagtaatct
     8461 gcttatgaac tttcttctca tacatcgtgg ttataaggga ggctgaatcg tcgatgccga
     8521 acgacagcct gttcagggtc acatcgaaca gactgtcagt attcttgatt ttcttgtcaa
     8581 gaaatgtctg tatcaggtca gccctctttt catcagtctc aactataaac ttctcggctt
     8641 cctcaatccc catattcttc ttcctcatta tattattcac cctccagtag aatggagcta
     8701 tcagtctgac gtgcagggcg tcctttatgt ctcgcgcaat tgaaacgccg ccccgcccca
     8761 caatgactat atacccttcc ttgcagattg aaagaacaac atcccttatg gcctttttta
     8821 ctctcagatc gcttacaaaa cctcctgaga aagccataat catctcagat agatcagaca
     8881 gcttgaaacc ctcataataa ttctcaatac gctgggtgtc aatattcaac tccttggcaa
     8941 ttgaatagat gatatcttta tccacccacc tccacttttc agttccgaat ttctcattta
     9001 atttctcgac aacctttgat gcaacctcac gggcatcgca tccggtaagc ctagaaatag
     9061 ttatgacagg accatcgttc tctgaaggag cttttaaaac tgatttcctg tatcgctcat
     9121 caaaataatc aaaaaatttt tccataataa gtgaaatttt ttcattctga tttttctctt
     9181 ctttgcacca agctgcataa ctgcaattga taaacttcct taaactgact ttgttcgttt
     9241 gatggcccgg aattaaagtt aaaagttaag aaaaaaaatt gagaaaaaga agtttttctc
     9301 atttcctccc gacagaggat gccacttatc cgacctgata tttttttatt tgtaatcatc
     9361 ttatttattc tatctttatc gcaatcattt acgatacttt aaattaattc aatcattatg
     9421 gattcccccc tgttatccag acctgaaata atatggttca taatcggcct tgccctcttc
     9481 ctgctggagc ttgtcctgcc aggatttgtg atattcttct ttggagtagg ggcttgggtt
     9541 accgcacttc tctgcctaat tgccaatccg ggcctgaacc tccaggttat tgtttttgca
     9601 gttacttctg tcctttcact tctggccctt agaagaatga tccagaaacg gttttttctt
     9661 gccagaaacg atggcctttc agacagtgtt gaagatgaat ttacaggaag ggaagccgtg
     9721 gcagttggca gtttcaaacc cggagaaact ggaaaagtcg actttaaggg aaccacctgg
     9781 aatgcagagt cggaatttta tataactgat ggacagagag ttattataat aaataaagag
     9841 agtttcaaac tattcgttga accaaaaaac aatcaaaaat gacagcagca attgcattgc
     9901 aaacagcatc aagtacaaca ttcatactga ttggaattat tttacttgcc ttcatcctca
     9961 tttcttcaat ggtgaaggtt gttccccaga gaacagccat gatcgttgaa agacttggaa
    10021 aatacagggc gacttttaca gcgggctttc aggtgctcat cccctttgtc gataagatac
    10081 ggtacaggca tacccttaag gaacaggcta tcgatgttgc atcccagacc tgcattacaa
    10141 aagacaacat tgccatcgag gttgatggca ttctttacct tcaggtcctc gatccccaga
    10201 aggcctctta cggtattgac aattacaggt ttgcatccat acagatcgca cagactacca
    10261 tgaggagcgt tataggcaag cttgagcttg accgtacctt cgaggaacgt gaatcaatca
    10321 atgcaacgat cgttgaggca gtcgataagg ccagtgaacc ctggggcgtc aaggtgacac
    10381 gatatgaggt gaaaaacatc attcctcccc agagtataaa ggatgccatg gaaaaacaga
    10441 tgagagccga aagggaaaaa agagcgctca ttgccgaatc agagggaacg aggcaggcaa
    10501 aaatcaatgt tgcagacggt gacaagcagg agttcatcct gagatcagag ggagaaaaac
    10561 agaaacggat aaatgaggca gcaggtcgtg catcagaaat tgaacagg
//
LOCUS       DAOOYG010000090        10368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6941_length_10368_cov_6.001164, whole genome shotgun sequence.
ACCESSION   DAOOYG010000090 DAOOYG010000000
VERSION     DAOOYG010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10368
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6941_length_10368_cov_6.001164"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1780)
                     /locus_tag="PK288_08255"
     CDS             complement(<1..1780)
                     /locus_tag="PK288_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPM18956.1"
                     /translation="MKRKITSLLLIILLGSFVYAQPDQKLKSDIRNTGYVHFPLPLDY
                     SKSFETFGLTKKVLVSSMLSDFETLAGWTHEGIGGMRLTTERSKSGKRSLRLVAPTMP
                     DSIPRRGLGRGSSYAVFNVGGKSWEKFNRIHLYVYPDCEGARSIYLNLYVENDGKIKV
                     PDIYGREGYHEMNLVNGQWNECFIEMTELARDKVTKLKFAIEVFGRELTMGDSLKFDI
                     DAIELQVVENPEVVSGWKPAANRIIYSTSGYRPESVKTAIVNVAGHKGKFELIDYTTS
                     QVVYEGSITPSKTRIGDFETIDFSDFRKEGEFIIRVGSVSTQPFFINRNLWDNSAWRV
                     CNFMFVERCGYPVPGKHGACHSDLNCEWNGKIYPVSGGWHDAADMSQQALQTGEIAYS
                     IMEMANRAREKNEIDLYHRLLEESLWGIDFIMKTRLGDGYRFQTWGTNLWTDKLIGTI
                     DDAGRRQGTIHNGGFENFLFSGIEAYASMSIDHDPMLKENLKKIAKEDFGFALARFEK
                     LGYNERINKGHGHAYMASQSQYMASVSWAASMLYKLTGEKYYADKAAEFIKYVIQCQR
                     TEPLNDKNGTRGFFYRDHEKKSIVHYN"
     gene            2189..2551
                     /locus_tag="PK288_08260"
     CDS             2189..2551
                     /locus_tag="PK288_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005848067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPM18957.1"
                     /translation="MENQVSFKEVKACPERYVLAISDTLNVLTGKWKLPIIGTLFYNK
                     KRFEEIKNNIPRITPGILSKELRELETNGIIKRVVPDSVPVAAEYELTDSGRNLKEVL
                     DRMLQWGIQHRDWVNASI"
     gene            2587..3771
                     /locus_tag="PK288_08265"
     CDS             2587..3771
                     /locus_tag="PK288_08265"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPM18958.1"
                     /translation="MGNSNRILSIDIMRGFTLVLMLFVNDLYMPGVPAWLGHVKADFD
                     GMGLADWVFPGFLFMVGMSIPYSIGNRIAKGESRTSLLIHIFIRTISLLVIGVLMSNS
                     RNVNAEYTGITKNIWSLIMYTGVFLIWNNYNESKLNRFLIMGLRILGVLLIIPMVIIF
                     RAGEDRGFYYFAWGILGAIGWGYLAAALVYLWFRNNILKTAFAVLFFLILNILSKLHL
                     LGFLNPVRQFLGIITGGSVPFMVLSGMLTTLILKKYVAENPGKTLLSIVTLGIAFIAA
                     GFILRPWFIISKIQATPTWGLICNGISMILFALFFWIIDIRKKVNWCLFLRPAGANSL
                     TVYLVPDMFYYIIWQFSLPLLFYKQLAEPVLVVMGSVVWAVIMGVGLPVLLEKINFRL
                     KL"
     gene            3796..4986
                     /locus_tag="PK288_08270"
     CDS             3796..4986
                     /locus_tag="PK288_08270"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HPM18959.1"
                     /translation="MANQNRVFSIDIMRGLTILLMLFVNDLNMNVAPRWLGHTRADFD
                     GIGLADVVFPGFLFIVGMAIPFAFAKRYSRGDSLVLISRHIITRTLSLLIIGVLMLNA
                     TRVNAELTGFSMELWALLMYLAVFLFWNDYPDKEERIYKIAGFKLAALAIFIFLIFKF
                     RSGQPENNGSLITGWWGILGLIGWGYLVAAFTYMVCRNSILRTTVAALFFLFLNIMSG
                     LRMLEFLNPVKPFLGVIIEGNVPFIVLTGLVAGLILQQNHAGRHGKVIITFTGLGVFC
                     LAAGFFLRKWFIFSKIFATPSWGLLCCGISFLAFTLIYWISDVKKKQTWAYFVKPAGE
                     NALTTYLAPDVLYFLIGMSGVPVLFYKSSENPLVVVVGSMIWALLMVGLTALLVRINI
                     KLKL"
     gene            complement(5052..7355)
                     /locus_tag="PK288_08275"
     CDS             complement(5052..7355)
                     /locus_tag="PK288_08275"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPM18960.1"
                     /translation="MKPTDKLEYLIFFLLVLLTGCGEREANKPDTQPADLVNPLMGTD
                     SEFSLSAGNTYPAIALPWGMNFWTPQTRGMNDGWIYTYDDHKIQGIKQTHQPSPWIND
                     YASFSVMAYTGSIKFSGEERASWFSHKSEIARPYYYSVYLADYDITAEISPADRSAIF
                     RFTFPQSDSSYIIIDAFDMGSMVKIIPGERKVIGYCRNNHGGVPSNFHNYFVAVFDRD
                     FTSAHVWNDGVLKTDTHDAAGGHVGALLRFKTVKGEKINVRISSSFISPEQASLNLSR
                     ELAGKDFETVRTEAGKIWNDQLSRIKIEGGTPDQQRTFYSCLYRTLLFPRKFYEINEK
                     DEIVHYSPYNGEVLPGYMFTDNGFWDTFRSLFPFLTLMYPELNSQIMEGLANTYRESG
                     WLPEWASPGHRDCMIGSNSASLIADSWLKGIRGYDIQTLYEAILKNTDSVGPVSSVGR
                     YGAKYYNDLGYVPYDAGINENVARTLEYAYADFCISRLAENLGRPQEEIGKFRQRAMN
                     YRNVFDPGRKLMRGRNLDGTFQSPFSPYKWGDAFTEGNSWHYTWSVFQDIEGLINLMG
                     GYDYFTAKLDSVFEVPPVFDYSYYGIIIHEIREMQIMDMGNYAHGNQPVQHMIYLYNY
                     AGQPWKAQYHVRNVMNKLYNYLPDGYCGDEDNGQTSAWYVFSALGFYPVTPGTDEYVF
                     GSPLFNKVTMQFENGRKLVIEASRNNARNIYVGDIRLNGRSLKENYIKHDQLQRGGRL
                     CFLMQDEPNMKRGIDRKAFPFSMSRSD"
     gene            complement(7596..8657)
                     /locus_tag="PK288_08280"
     CDS             complement(7596..8657)
                     /locus_tag="PK288_08280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPM18961.1"
                     /translation="MRKSLTKNIEMRLLVLRTSSMGDVALLSPVLGGFAEQFGTVNIL
                     FVTRKTFEPFFRHSADLQFFFPDFKGRHKGLTGIFRLFRDLRKTGRIDHVVDLHDVIR
                     TRILRLLFVLTGTRFHKIDKGRREKRWLIKGKIRSQLKHTVTRYAETFAKAGYSLTLP
                     GGPWIFPSPLSAANAEKVTGISDGMNIGVAPYAMHQLKMWPEEYMIELIKKISERYKV
                     RFWLFGGADEAQKLELLRQKIPGSFLTTGRLSLEEELALMKRLSFMISMDSSNMHMAS
                     LAGTKVISIWGATDPATGFGAWGQPEEFSVRIPAAELTCRPCTVYGKGSCRRGDHACM
                     RWLTPELVFEKLLMTGLVK"
     gene            complement(8629..9000)
                     /locus_tag="PK288_08285"
     CDS             complement(8629..9000)
                     /locus_tag="PK288_08285"
                     /inference="COORDINATES: protein motif:HMM:NF039364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HPM18962.1"
                     /translation="MKIEVSNGEIIDKLTIIQIKLERIKDPGKLKNLNREYEILRNAS
                     ASVLSQDDPLYLELYKVNNDLWDIEDKIRDLEKRKDFGNDFIQTARSVYFINDKRSEI
                     KREINLKTSSDLFEEKSYEKY"
     gene            complement(9167..>10368)
                     /gene="priA"
                     /locus_tag="PK288_08290"
     CDS             complement(9167..>10368)
                     /gene="priA"
                     /locus_tag="PK288_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPM18963.1"
                     /translation="YHARDTAIVLAGLHGAHALLGSASPSVESYHNSVTGKYGLVELK
                     ERYGFVNLPEIILANTRESYRKKLMISHFTPELLEAMDEALNNKEQIILFQNRRGFAP
                     YLECPECGWVPKCNQCSVNLTYHKGQNRLICHYCGYFQPLPAHCGNCNSPGLVTRGFG
                     TEKIEDEIRIVFPKARVARLDQDTTKGRDAFNRIISAFEQNRTDILIGTQMISKGLDF
                     ENLTVVGVLNADNLLNFPDFRAHERSFHLLEQVSGRAGRRKKQGRVIIQTADPDNKII
                     RLVLKHDYYNLFRLQAEERQAFNYPPFCRMIRISLKHKDRAALNGFSDILGNDLKSVF
                     GKRVLGPEYPLISRIQSWYIKTILIKLGRDKSAVNAKLVIAEAIARLEKEKGASSLRI
                     AIDVDPY"
BASE COUNT         2673 a         2498 c         2146 g         3051 t
ORIGIN      
        1 ggttgtaatg cactattgac ttcttttcat gatcgcggta gaagaatcct cttgttccgt
       61 ttttatcatt caatggctca gttctctgac actgaatgac atatttaatg aattcagcag
      121 ccttgtcggc ataatatttc tcgccggtaa gcttgtaaag catgctggct gcccacgaca
      181 cagaagccat atactggctt tgcgaagcca tataagcatg tccgtggccc ttgtttatcc
      241 tctcattata gccgagcttc tcaaaccttg caagtgcgaa tccaaaatcc tctttggcaa
      301 tctttttcag attctccttc agcataggat catggtcaat gctcattgag gcgtaggctt
      361 caatacctga aaagaggaag ttttcaaaac cgccgttatg tatggttccc tgacgacgtc
      421 cggcatcatc tattgtacct atcagtttat cggtccaaag atttgtaccc caggtctgga
      481 acctgtatcc gtcgcccagc ctggttttca ttataaaatc aatgccccac aatgactctt
      541 ccagcaacct gtgatacaga tcgatctcgt ttttctccct ggccctgttg gccatctcca
      601 ttattgaata ggcaatttct ccggtctgca gagcctgctg cgacatatcg gcagcatcgt
      661 gccaaccgcc gctgacagga tatatctttc cgttccattc gcagttgaga tctgaatggc
      721 aggccccgtg cttacccgga accgggtagc cgcacctttc cacgaacatg aaattgcaaa
      781 ccctccacgc cgaattatcc cacaaattcc ggtttataaa aaatggctgg gtggaaactg
      841 atccgaccct gattatgaat tcgccttctt tcctgaaatc gctgaagtcg attgtctcaa
      901 aatcaccgat acgcgttttg gaaggtgtta tgcttccttc atacacaacc tgcgacgtgg
      961 tataatcaat aagctcaaac ttgcccttgt ggccggctac atttacaatg gctgttttca
     1021 cgctttccgg cctgtaacct gaagttgaat agatgatcct gtttgcagcg ggtttccagc
     1081 cactgacaac ctccggattt tcaacaacct gcaattctat tgcatcaatg tcgaatttca
     1141 gtgagtcacc catggtaagc tcccttccga aaacctcgat tgcaaatttc agtttggtaa
     1201 ccttatccct ggccagttcg gtcatctcaa tgaaacactc gttccattgc ccgttgacaa
     1261 ggttcatttc gtggtatcct tccctgccgt agatatccgg aactttgatt tttccgtcgt
     1321 tctcaacata cagattcaga taaatgctcc ttgcaccttc acaatcggga taaacataca
     1381 aatgtatcct gttgaatttc tcccagctct tcccgccgac attaaaaaca gcgtaggatg
     1441 atccccgtcc cagccccctt cttggtatgc tgtcgggcat tgtgggagct accagcctca
     1501 ggctcctctt tccgctcttg ctcctttcag tagtaagccg catcccccct atcccttcat
     1561 gagtccatcc cgcaagtgtt tcaaaatcac tcagcatgct ggaaaccagc acttttttgg
     1621 taagaccaaa ggtctcaaat gatttgctgt agtccagcgg aagcgggaaa tgcacataac
     1681 ccgtgttcct gatatctgac ttcagcttct ggtcaggctg ggcatataca aatgacccaa
     1741 gtaaaataat caacaatagt gatgtaattt ttcttttcat agtcaggtag tatttttaaa
     1801 atgttaagtt aattattaaa tggtcctgat acaaattctt aggttggcag cttcagcttt
     1861 ttaattattt ttaagtttta tttcccttat gtgacagggc ttcagtaacg tagatccggc
     1921 atcctgttcc agcctggtgt taatgctttc acacaatctg atttcaacga tctttttgca
     1981 ttttccagtt cctccagatc ctacggctcc ctcttccttc acgccaaacg actcagtata
     2041 attctccccc cccaaaaata acaaaagggg aatcttaaac ctgttccgtt cagaagcaaa
     2101 aatattgcat atttgccttg aaaaaagctg tgctatactt ttgtatacct tggtataatc
     2161 aaggtgaaat gagatttaca tttgtgttat ggaaaaccag gtcagtttta aagaggtcaa
     2221 agcatgtccg gagaggtatg ttcttgccat atccgatacc ctgaatgtac tcaccggaaa
     2281 atggaaactt cctataatcg gtactctttt ttataacaag aaaagatttg aagaaataaa
     2341 aaacaatata cccaggatca ccccggggat tctttccaaa gaacttaggg agctggaaac
     2401 taacgggata ataaaaagag tagtaccaga ttcggtaccg gttgctgcgg aatacgaact
     2461 gaccgattct ggaaggaatc tgaaagaagt tcttgacaga atgctgcaat ggggcataca
     2521 gcacagggat tgggttaatg caagcatata aagacatcac aaacacaaat ttataatttc
     2581 agatcaatgg gtaacagcaa ccgtatactg tcaatcgaca tcatgagagg ttttacactg
     2641 gtactcatgc tgtttgttaa tgatctttat atgccgggcg tacctgcatg gctgggtcat
     2701 gttaaggctg acttcgacgg gatgggtctg gccgactggg tttttccagg attcctgttt
     2761 atggtaggca tgtcgatccc ttattccatc ggcaacagga ttgcaaaggg agaaagcagg
     2821 acatctctcc tcattcacat ttttatcaga accatcagtc tccttgtaat cggggtatta
     2881 atgtccaatt ctagaaatgt caatgcagag tataccggga taaccaaaaa tatctggtcg
     2941 ctgataatgt acacaggggt gttccttatc tggaacaact acaacgaatc gaaactgaac
     3001 aggtttctga taatgggcct caggattctg ggtgtgctgc tcataatacc gatggtcatt
     3061 attttcaggg cgggcgaaga caggggtttt tactattttg cctggggtat cctgggagca
     3121 atcggatggg gttacctggc agccgcactg gtatatttgt ggttccggaa caacattctt
     3181 aaaacagcct ttgccgtatt atttttcctt attctcaata ttctttctaa gctccatctg
     3241 ctgggttttc tgaatccggt caggcaattc ctgggaataa taaccggcgg aagcgttccc
     3301 ttcatggtcc tttccggcat gcttacaact ctgatcctta aaaaatacgt tgctgaaaat
     3361 ccgggaaaga cattgctttc catagtgaca ctgggaatcg cattcatagc ggcaggattc
     3421 atcctccggc cctggtttat aatctccaaa atccaggcaa cacccacatg gggtcttatc
     3481 tgcaatggta tcagcatgat cctgtttgca ctatttttct ggataattga catccggaag
     3541 aaagtaaact ggtgtctttt tctcagaccg gccggagcca attcactgac ggtatacctc
     3601 gtacccgata tgttttatta catcatctgg caattctcac tcccgctgtt gttttacaag
     3661 caactggcag aaccggttct tgtagtcatg ggatccgtgg tctgggccgt gataatgggc
     3721 gtcgggttgc cggtacttct ggaaaagatt aatttccggc ttaaactcta atgttatatt
     3781 tttcacatta atccaatggc aaatcaaaac cgtgtttttt cgatagatat tatgcgggga
     3841 ctgacaatcc tgctgatgct tttcgtcaac gatctaaaca tgaatgttgc acccaggtgg
     3901 ctgggacaca ctcgagccga ttttgacgga ataggacttg ctgatgtagt atttcccggt
     3961 ttccttttta ttgtcggaat ggccattcca tttgcgtttg caaaaagata ttcccggggt
     4021 gattcactcg ttctgatcag ccgccacata ataacacgaa ctctcagcct gcttatcata
     4081 ggagttctaa tgcttaatgc tacaagggtc aatgctgaac ttaccggctt cagcatggaa
     4141 ctctgggcac tgctgatgta cctggccgtt ttccttttct ggaacgatta tcccgacaaa
     4201 gaagaaagga tatataaaat tgccggattt aaactggcag cactggctat ctttattttc
     4261 ctgattttca aattcagatc cggccagccg gaaaataacg gctcgctcat caccggatgg
     4321 tgggggatcc tcgggctgat aggttggggt taccttgttg cagcatttac ctacatggtt
     4381 tgcaggaatt ccattctgag gacaacggtt gctgcactct ttttcctgtt cctgaatatt
     4441 atgtcaggcc tgagaatgct tgaattcctg aatccggtga agcctttcct cggtgtcatt
     4501 attgaaggga atgttccatt tatagttttg accggactgg ttgccggcct gatccttcaa
     4561 caaaatcatg caggcagaca tggaaaggta attatcacat ttacaggact cggagttttc
     4621 tgcctggcag caggattctt cctaagaaag tggttcatct tctcaaaaat ttttgcaaca
     4681 ccaagctggg gactgctatg ctgcggcata agcttcctgg cattcacgct gatctactgg
     4741 atatcagatg taaagaagaa acagacctgg gcctattttg taaagcctgc aggagaaaac
     4801 gctcttacaa cctatctggc acccgatgtt ctatattttc tcatcggtat gtcgggtgtt
     4861 ccggtattat tttacaaaag ctctgaaaat ccccttgtcg tagttgtcgg ttcaatgatc
     4921 tgggctttgc tcatggtagg actcacagct ctcctcgtac ggatcaatat aaagcttaaa
     4981 ctgtgacctt tccgctaccc ggaatttttt tagaaagaag cttccggcta atgcttaaag
     5041 gtcatattac tctaatctga ccggctcatt gagaaaggaa atgccttcct gtcaatgcct
     5101 cttttcatgt ttggttcatc ctgcatgagg aaacacagcc tgcccccgcg ctgaagctga
     5161 tcatgcttaa tataattctc tttcaatgat ctgccattca gcctgatatc tccgacataa
     5221 atattcctgg cgttgttccg ggaagcttca atgaccagct tcctgccatt ttcaaactgc
     5281 attgtaacct tgttaaataa cggtgatccg aaaacatatt catcagtgcc cggggttaca
     5341 gggtaaaatc ccaatgccga aaacacatac caggccgatg tctgtccgtt atcctcatca
     5401 ccgcaataac cgtcgggaag ataattgtac agcttgttca tcacgttcct gacatgatat
     5461 tgcgctttcc agggctgccc tgcatagttg tagagatata tcatatgctg cactggctga
     5521 tttccgtgag cataattacc catgtccatt atctgcattt ccctgatttc atgtataatg
     5581 atgccatagt aggaataatc aaacaccggc ggaacttcaa atacggaatc aagcttggca
     5641 gtaaaataat catatcctcc catcaggtta ataagtcctt caatatcctg gaacacgctc
     5701 catgtatagt gccagctgtt gccttcggta aaagcatctc cccatttgta tggactgaaa
     5761 ggcgactgaa aagtaccgtc aagattcctt ccgcgcataa gctttctgcc aggatcaaat
     5821 acattcctgt agttcattgc cctttgcctg aacttgccaa tctcttcctg cggtcgaccg
     5881 agattctcgg caagccttga aatacagaag tctgcataag catattcaag tgtccgtgca
     5941 acgttttcat ttatgcctgc atcatacggc acataaccga gatcattgta atactttgca
     6001 ccatatcgcc cgactgaact aacaggacct acgctgtcag tgtttttcag tattgcttcg
     6061 tataaggtct gaatgtcgta tccccttatt cctttaagcc aggaatctgc tatcagtgat
     6121 gcagagtttg agcctatcat gcagtcgcga tgtccggggc tcgcccattc aggaagccag
     6181 ccgctttccc tgtatgtatt tgcaagacct tccattatct gactgttcag ttcgggatac
     6241 atcagggtta aaaatgggaa caacgatctg aaagtatccc agaaaccatt gtcagtgaac
     6301 atgtacccgg gcagaacttc accgttatat gggctgtagt gaactatttc atccttttca
     6361 ttgatctcat agaacttcct cgggaaaagc agcgtacggt aaagacatga atagaatgtt
     6421 ctctgctggt caggtgtgcc tccttcaatt ttaatccggc taagctggtc attccagatt
     6481 tttccggcct cggtacgcac tgtctcgaaa tcttttccgg ccagctccct cgacaggttc
     6541 agggaagcct gttcggggct aataaaggaa gatgagatcc ggacattgat tttttcgccc
     6601 tttacagtct taaacctcag cagggcccct acatggccgc ccgcagcatc atgtgtatca
     6661 gtcttcagaa caccgtcatt ccaaacatgt gccgaggtaa aatccctgtc aaaaacggcg
     6721 acgaaataat tatgaaagtt tgacgggacg cccccgtgat tgttcctgca gtatcctatt
     6781 acctttctct ccccaggtat tatcttcacc atcgatccca tatcgaaagc atcaatgatg
     6841 atatatgacg aatcactctg cggaaatgta aagcggaaaa tggcagagcg gtcagcaggc
     6901 gaaatttctg cggtaatgtc ataatctgca aggtaaacgc tatagtaata tggtcttgca
     6961 atctccgact tatgggagaa ccatgaagcc ctctcttctc cactgaactt aatactgccc
     7021 gtataagcca tcactgagaa tgaagcataa tcatttatcc agggacttgg ctgatgtgtc
     7081 tgcttaatac cctgaatttt atggtcatca taggtataga tccatccgtc attcatgccg
     7141 cgcgtttgag gagtccagaa attcataccc cacggaaggg caatggcggg ataggtattc
     7201 ccggcagaaa gactgaactc ggaatcagta cccattagcg gattaaccag gtcggccggc
     7261 tgcgtatccg gtttatttgc ctctctttcg ccgcatcctg taagaaggac cagcagaaaa
     7321 aatatcaagt attcaagctt gtcggttggt ttcatatgca aatctttgtt ttcagaatga
     7381 aaatggaaaa tctttcggta aaagatccat acgataaatt atttcatgaa tcgtgtgctt
     7441 tattcacagt tttcaaacag aatcagagga ttgaaaatca atggttctgg tcattaaagg
     7501 ggtctttccg gtcagcctaa ttatatttct tttttctttg agtaatatgt tctaaaatac
     7561 cagaataatc tgataaactg aaatatttat ctcggttatt tcactagtcc tgtcataaga
     7621 agtttttcaa acaccagttc aggtgtaagc cacctcatac aggcatgatc tccccttctg
     7681 cagcttccct ttccatatac agtacagggc cggcatgtaa gttccgcggc tgggatcctg
     7741 acagaaaact cctcaggctg cccccaggcg ccgaagcctg tcgcgggatc agttgcaccc
     7801 catatggaaa tcacttttgt acctgccagg gaagccatat gcatgtttga agagtccatt
     7861 gatatcatga aactcagtct cttcatgagt gcaagttcct cttccagcga cagcctgcct
     7921 gttgtcagaa acgatcctgg tatcttttgc cggagtaatt caagtttttg tgcttcatcg
     7981 gcgccgccaa aaagccagaa gcggacctta tatctctcac tgatcttctt tataagttca
     8041 atcatgtatt cttctggcca catcttgagc tgatgcattg catacggagc cacgccaata
     8101 ttcattccat cagatatccc tgttactttt tccgcgtttg cagccgacag cggggaagga
     8161 aagatccagg gccccccggg cagggtcagg ctatatcctg cttttgcaaa cgtttcagca
     8221 tatctggtaa cagtatgctt aagctgtgac ctgattttcc cttttatcag ccaacgtttt
     8281 tccctcctcc ctttgtcaat tttatggaac cgggttccgg tcagaacgaa aagtaaccta
     8341 agtatcctgg tccttataac atcgtgcaga tccacaacat ggtcaattct gccagtcttc
     8401 cttaaatcgc ggaaaagacg aaaaatcccg gtcaatccct tgtgcctgcc cttaaaatca
     8461 gggaaaaaga actgtaaatc agctgaatgc ctgaagaaag gttcaaaagt tttccttgta
     8521 acaaacagaa tattcacggt accgaactgc tccgcgaatc ctcccagaac cggagacagc
     8581 agggcaacgt cgcccattga ggacgtccga agcacaagca ggcgcatttc aatatttttc
     8641 gtaagacttt tcctcaaaaa gatccgacga ggttttcaga ttaatctccc gctttatctc
     8701 cgatctttta tcgttaatga aataaacgga ccgggctgtc tggataaaat cattaccaaa
     8761 gtcttttcgc ttctcaagat ccctgatctt atcttcaatg tcccaaaggt cattattaac
     8821 cttgtacaat tccagatata aagggtcgtc ctggcttaaa accgacgcgg atgcatttct
     8881 gaggatctca tattccctgt tcaggttttt cagttttcca ggatccttta tcctttcgag
     8941 ctttatctga attattgtca gtttgtcaat tatttcaccg ttcgatactt ctatcttcat
     9001 ctatctcgtt ttagcctgat caaatttagt ctctttttat aaaggaacaa acccacttcc
     9061 tgatatccgg tccggataat catccggctc agtatattca tccgtatcat cgcagaattt
     9121 ggtatcacct gctctcctta tatgtgatgc aggccagatc agggctctaa tacgggtcta
     9181 catcaattgc tattctgagt gaagaggcgc ccttttcctt ttccagccgg gcaattgctt
     9241 ccgcgatcac aagttttgca ttaactgctg acttatccct accgagcttt ataaggattg
     9301 ttttaatgta ccatgactgg atacgtgaaa tcagcggata ttcgggccca aggacccttt
     9361 tgccaaatac tgatttcagg tcatttccga gaatatccga gaaaccattc agtgctgccc
     9421 tgtctttgtg cttcaggctt atcctgatca ttctgcagaa tggaggatag ttgaatgcct
     9481 gcctttcttc ggcctgcagc ctgaagagat tgtaataatc gtgtttcagg acaagcctta
     9541 ttattttgtt gtcaggatct gccgtttgta taataacccg tccctgtttt ttcctccttc
     9601 cggcccgccc gcttacctgc tccagaagat gaaagcttct ctcatgtgcc ctgaaatcag
     9661 ggaaattaag aaggttgtct gcattcagca cccccacaac ggtgaggttc tcaaagtcaa
     9721 gtcccttgga aatcatctgg gttccaataa gaatatcggt acggttctgt tcaaaggcag
     9781 aaattatcct gttaaatgca tccctgcctt ttgtcgtatc ctgatcaagc ctggccaccc
     9841 gtgcctttgg aaaaactatc ctgatttcat cttcaatttt ctccgtgccg aatcctcttg
     9901 ttacaagtcc cggagaattg cagttcccgc aatgtgcagg taacggctga aaataaccgc
     9961 aataatggca tatcagcctg ttttgtccct tgtgataggt caggttcaca gagcattgat
    10021 tgcatttcgg gacccagccg cactccgggc attccaggta cggggcgaac cccctcctgt
    10081 tctgaaaaag aatgatctgc tccttattgt tcaaagcttc atccatcgcc tcaagaagct
    10141 cgggagtaaa atgagagatc atcagttttt tccggtacga ctccctggtg tttgccagaa
    10201 tgatctcagg caggttaaca aatccgtacc tttccttcag ttcaaccaat ccatacttcc
    10261 cggtgactga attatgatag ctctcaacag aaggggaggc cgatccgagc agcgcatggg
    10321 caccatgaag gccggcaagg acaatcgcag tgtcgcgtgc atggtatc
//
LOCUS       DAOOYG010000091        10316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_6984_length_10316_cov_6.543319, whole genome shotgun sequence.
ACCESSION   DAOOYG010000091 DAOOYG010000000
VERSION     DAOOYG010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6984_length_10316_cov_6.543319"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PK288_08295"
     CDS             complement(<1..1297)
                     /locus_tag="PK288_08295"
                     /inference="COORDINATES: protein motif:HMM:NF003007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HPM18964.1"
                     /translation="MEWFGDLVWNDSVAHTVLLYCVIIILGVFLGKRKIFGISLGITF
                     VLFAGIAIGHLGFTANKYVVDFLRDFGLILFVFFIGLQVGPAFFSSFRKGGLSLNIIA
                     LIIVILGAVTTITIHFVTGTSMPMLAGIMSGAVTNTPGLGAAQTALSQMMEKIPGAEI
                     PEISLGYAIAYPFGILGVILSMIFIKRIFRIDLAKEIAAFEEELHPSDTLPEKASILV
                     RNPDLFNRSILEISKSLKPGVVISRILHNGNLHAATSETIIHENDIVLVVAQRSLLEE
                     IIGTFGTLSSMDLASDPGQLISRRILVTNGDVVGRSLGSLKLRTLYNINITRIHRSDI
                     EIIASPGLRIQMGDRLTVVGEEKSVMLVADQLGNSLKRLKEPNVIALFTGILMGVILG
                     SIPFNIPGVSHPIRLGLAGGPLIVAILMSRFGYKISLISY"
     gene            complement(1583..3721)
                     /locus_tag="PK288_08300"
     CDS             complement(1583..3721)
                     /locus_tag="PK288_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM18965.1"
                     /translation="MNIEKTDHGRNGDSILPGTGITWRTASHGARIGSLPSMLLTVSL
                     LMLPGFIIAGGKTLQEPDTLVSPALSEVVVSASRFSSRVVNTPDAVRVLNNTAIRENQ
                     SRSVPEALSLTPGVFVQKTNHGGGSPFIRGLTGNQTLLMVDGIRLSNSVMRYGPNQYF
                     NTIDVFSLGKIEVLRGSGSVQYGSDALGGTIQAFTPELKISSDPVWNTRFLARFGTHG
                     MEKSFNLNSGYSSKSFALRGGLTYRDFGDLVGGDTTGRQTPSGYREFDYDVKGKIMIS
                     GKSSVTFAFQNVHQKDVPVYHKVVLENYAVNKMNPQIRKLAYARLNQDLNRGILKSAV
                     ITGSYQFTDEEREMRKNGSATLRKEADKVGTYGFSGELLFSSSTAWNGNIGFEAYSDR
                     VRSSRADIDLQTSGTVLKRGLYPDGSSMTSLAVFSTHAVDIKNWILSGGARFNAFSIK
                     VSDETIGQTKIKPVAVVGNIGILRKLTPDDGLFVSLNTGFRAPNIDDLGTLGIVDFRY
                     ETPNFDLKPEKSLQYQLGYRHNGKVLKGEFFAYRNELYDLITRKRVENQTIEGYQLYQ
                     KENSDRAYIHGLETSWEVYAGSSLVIQGSLTYTYGQNITNDEPLRRIPPLFGRLAADY
                     RHKGYLFGIEWLAASKQSRLARGDVDDNRIPEGGTPGWNIFNLNTGYSFKFLDIDLAF
                     RNLLNKDYRYHGSGINGIGRSVFLTIGINL"
     gene            complement(3798..4754)
                     /locus_tag="PK288_08305"
     CDS             complement(3798..4754)
                     /locus_tag="PK288_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPM18966.1"
                     /translation="MKSNTNRRKFIKKGAIMAAGMAAMSFRSIKALPGSQADFPVRLG
                     GPVPGEFSDPADWAAAVKKLGYSAAYCPLQPGAPEDLIRAYRAEARRKNIIIAEVGVW
                     NNMLHDDEKTRKENLEKNMEALHLADRIGAVCCVNISGARGEIWDGPYPGNYAKDTFD
                     LIVENIRSIIDQVKPVNTFYTLEPMPYMLPDSPDSYLDLIKAIDRKQFAAHLDPVNMI
                     SSPQRYFNNAAFLKECFRKLGPYLKSIHAKDIIILPRLTVHLEERRPGLGALDYSVFL
                     GEAVKFRDLPFMMEHLETQDEYLLAAGYIRNIGEKNGIRFVE"
     gene            complement(4770..5906)
                     /locus_tag="PK288_08310"
     CDS             complement(4770..5906)
                     /locus_tag="PK288_08310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPM18967.1"
                     /translation="MNKMLRISVLIIIVTGFFHTALPQADHPEPKTLAIGSKAPDFSL
                     PGIDGKTWTLRDFSKAPVLVIIFSANHCPTAQAYEDRIISITNDYKSRGVALVMISSN
                     SVRTLSYSEMGYTDVGDSFEDMKYRAGDKGFNFPYLYDGDDQKCALAYGPVATPHCFV
                     FDRDRILRYNGRIDGSEKPGSAGGEDLRKAIDDVLAGRPVGTPVTKTFGCSIKWAWND
                     DYTRRLYKQWAELPVTIEEIDVAGLKELVRNSGSGKLRLINFWATWCGPCITEFPDLV
                     IIDRMYRGRQFEFITVSADKKARKADALRFLQKNEASNRNYIFNDDDIYKAIEAVDPD
                     WQGALPFTMLIEPGGKIVYKKQDVIDPLKMKKLIVENKYIGRYY"
     gene            6031..6315
                     /locus_tag="PK288_08315"
     CDS             6031..6315
                     /locus_tag="PK288_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18968.1"
                     /translation="MASRKPVAGFIGAGGIAHSHAYALDAMKHYYHDAPEIEKEAVSS
                     ATSSGRESFANRYGFRSSLCPGDFFENGRINSVFILGPNNTHILSISGKP"
     gene            6312..7130
                     /locus_tag="PK288_08320"
     CDS             6312..7130
                     /locus_tag="PK288_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18969.1"
                     /translation="MMMPSVERIYIEKPLCVSSDEESVIRRIAQERASIKIQAGYQFL
                     FSSAVREALRFWRSGVLGKPVHFDFRYYHGDYLARDYRNRRRTRLMPAPEGGAMADLG
                     SHIISMAIAFIGNNMDVTSAVQAGSFDDVPADSDLFSLITLHDPESGAAGVISASRVS
                     SGTGDMLSFELHAEKGSLKYSSHFPDFFEYYTEDSGIWHKISTGSSYKPVSSFPSGHV
                     PPGWLRALVHAHYVFFTSGESESMLPDLEHGLEVERILRESAGQMRIFRNRVCR"
     gene            7280..7861
                     /locus_tag="PK288_08325"
     CDS             7280..7861
                     /locus_tag="PK288_08325"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HPM18970.1"
                     /translation="MAAFIDEFMKTYGPEVTRQMSASFDLDQDTVQKLIPQLAPLILG
                     GLKRQKDDRGGDARVDHILNKYGDQSVLDNIRDLMASKASASSVDANLGGLLGSMGGV
                     QAAQVLGKSMNIDASKIMKMIPALAPLILGALTRRRDTGGGGISGIGSLLDANGDGSI
                     IDDVAGFLIKGTASQGKGKGILGSLLGGMLGTK"
     gene            8124..9629
                     /locus_tag="PK288_08330"
     CDS             8124..9629
                     /locus_tag="PK288_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM18971.1"
                     /translation="MVSSGKINRRKFLGSAAAASLAFTIVPRNVIGGKGHVAPSDKLT
                     LAYIGCGTQGLREMVTLIQNPDIQIVTVCDPNKFTTDYVDWSHDSIRNSIRKALQDAS
                     WGEKIKGIPGGRDIGQEFVQKYYAKIRNLSGYKCPSYSDFRELLEKEKDISSIKIMTP
                     DHLHAAIAIASMKKGKHVVTHKPIANRMNEARLTIETAKKSGLSTHLLAWSRRPEYEI
                     IRDWIKDGFIGTLKEIHNWSYRPVWPQWQSNPTDTPPVPKGFDWALWLGPVPDRPYHP
                     NYTHNVFRGWYDFGGGSIADMGHYSLFPLFITLGINTPAKTAEAYGTTTRIIKDNVST
                     AVVNDVAFPLSCMIRFEFPAQPALPPFDLIWYDGGIKPLVPDELRAAGKTLATEGMMF
                     VGDRGKILAGFRGENPILLPESKMNYLKKQEEDESERRSDKIWIDAFKNGTQPDGSFI
                     YAGPVTETILLGAVALRAGKKVEYDPVNMKITNVPEANKYLTREYRPGWEL"
     gene            9681..>10316
                     /locus_tag="PK288_08335"
     CDS             9681..>10316
                     /locus_tag="PK288_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPM18972.1"
                     /translation="MNEISRRSFLEKSIVAGATAMITPSVLKSAAPALTPRMTRDDIS
                     LAQWALVEEIRAGKWKTLDFPSVARKDFGLNGIEFVNTLFEVPTEGYLRRLKKNAEDN
                     NVTMVLIMVDDEGDGCSPTREGRKQFDINHRKWIDIAHFLGCSAIRTNCRGPKDVNKD
                     EALKYSVESYQMLLEYAVPAKISVLIENHGGISDDADWMVSLFKAVNNLYFG"
BASE COUNT         2788 a         2462 c         2283 g         2783 t
ORIGIN      
        1 tgtaggaaat cagtgaaatc ttgtacccga atctgctcat cagtattgca acgatcagcg
       61 gtcccccggc aagtcccagc ctgatggggt gtgaaacgcc gggaatattg aaggggatgc
      121 tgcccaggat aactcccatc agaatccctg taaacaatgc tatgacattg ggctctttga
      181 gccgtttcaa tgagttacca agctgatccg ccacaagcat taccgacttt tcctctccca
      241 caacggtaag cctgtcgccc atttgtattc tcagccccgg agaggcaatg atttcaatgt
      301 cagacctgtg aatccttgtt atgttgatat tatacagggt tctgagtttc agggatccaa
      361 gtgaacgacc aaccacatca ccattggtga ccaggatccg ccgtgatata agctgacccg
      421 ggtctgaagc gagatccata ctgctcagtg tgccgaacgt accaataatt tcttccaaaa
      481 gggatctctg agccaccaca agcacaatgt cattttcatg aatgattgtc tcggaagtag
      541 cagcatgcag attcccgtta tgcaatatcc ttgagattac aacaccgggc ttaagggatt
      601 ttgatatttc cagaatggac ctgttgaaca aatccggatt cctgaccagt atgcttgcct
      661 tttcaggcaa agtatccgat ggatgaagct cttcttcaaa tgcggcaatt tctttcgcaa
      721 gatcaatgcg aaagattctt ttaataaaaa tcattgacaa tataactcca agtatgccaa
      781 agggataagc tatggcatat cccaggctga tttccggtat ctccgcaccg gggatttttt
      841 ccatcatctg ggaaagtgcg gtctgggcag ccccaagccc gggagtattt gtaacagctc
      901 ctgacattat cccggcaagc atcggcatgg atgttcccgt aacaaaatgt attgttattg
      961 ttgtaacagc acccaggata actattataa gtgctattat attcagcgac aatcctcctt
     1021 ttctgaagga ggaaaagaat gcgggaccaa cctgaagtcc gataaaaaat acaaaaagga
     1081 tcagtccgaa gtcccgtaaa aaatccacaa catatttatt ggctgtaaaa cccaaatggc
     1141 caatggcaat cccggcaaac aaaacaaagg ttatccccag tgaaatcccg aatattttcc
     1201 tctttccaag aaacaccccc agtattatta ttacacagta caacaatacg gtatgagcaa
     1261 ctgaatcatt ccagacaaga tcaccaaacc attccatctt caattacttt tattttgtag
     1321 gtaatttccg tttccggttt tatttttctt cagggttttc cccctccagg gtcacagtcg
     1381 cttttaaaag ctttacggat gtgagaaata tgtaaaaaaa attatttcct cagatcagga
     1441 cacttcaaag ctttcatcaa tccaggtgaa ataaaagatg gtaaacaagg caaacaccat
     1501 tctgaattta aaaagcccgg caaaaccatt ttcccggatt aacattacct gaatcaaatg
     1561 atgaaatcac agcccggacc tttcacagat taatcccgat agttaaaaaa accgatcgtc
     1621 cgatcccgtt tatccccgat ccatgatacc tgtagtcctt atttaaaagg tttctgaaag
     1681 ccaggtcaat gtcaaggaat ttaaatgaat agccggtatt aaggttaaaa atgttccagc
     1741 ccggagttcc accttcaggt atcctgttgt catcaacatc tccccgggcc agcctcgatt
     1801 gttttgatgc ggccagccat tcaatcccaa acaggtatcc cttatgcctg taatcagccg
     1861 caaggcgtcc gaaaagcggg ggaatacgtc ttaagggctc gtcatttgta atgttctgtc
     1921 cgtatgtata ggtcagggaa ccctggatga ccaatgaaga gcctgcatat acctcccatg
     1981 atgtttccag accatgaata tatgccctgt cactgttctc tttctggtac agctggtatc
     2041 cctcgattgt ctgattttct actcttttcc gtgttataag gtcgtaaagt tcattccggt
     2101 atgcaaaaaa ctcgcctttc agtaccttac cgttatgcct gtaacccaac tgatactgaa
     2161 gggatttttc gggcttaagg tcaaaattgg gagtctcata cctgaagtca acgatgccca
     2221 gtgtgccgag atcatcaata tttggagccc ggaaaccggt attgagtgac acgaacaatc
     2281 cgtcgtcggg agtcagtttc ctcagaattc cgatattacc cacgacagcg accggtttta
     2341 tttttgtctg acctatagtt tcatcactta cttttattga aaaagcattg aaccgggctc
     2401 ctcccgacag gatccagttt tttatgtcaa cagcatgggt tgaaaataca gccaaactgg
     2461 tcatcgatga tccgtcggga tataagcccc ttttcaaaac cgttccgctg gtttgaaggt
     2521 caatatccgc ccttgaactt cttacccggt cagaataggc ttcaaaacct atattcccgt
     2581 tccaggctgt tgaagatgaa aacagcagtt ctccggaaaa accataggtt cctactttat
     2641 cagcttcttt tctcaaagtt gccgatccgt tttttctcat ttccctttct tcatcagtaa
     2701 actggtatga acctgttata actgcggact tgagaatacc cctgttcaga tcctgattca
     2761 gtcttgcata ggcaagttta cgaatttgag gattcatttt gttcaccgca taattttcaa
     2821 ggacaacctt gtgataaaca ggaacatctt tctgatgaac attctggaat gcaaaagtta
     2881 ccgatgattt gcccgaaatc attatttttc ccttgacatc ataatcaaac tcccggtatc
     2941 ctgaaggggt ttgccgtccg gtggtgtccc ctcccaccag gtctccgaaa tccctgtatg
     3001 tcaaacctcc tctcagtgca aaggatttac tgctgtaacc tgaattcagg ttaaaacttt
     3061 tttccatgcc gtgggtgccg aatcttgcaa ggaatcttgt attccataca gggtccgatg
     3121 aaatcttcag ttcaggggta aatgcctgta ttgtaccgcc cagtgcatca gatccgtact
     3181 gtaccgatcc gctgcctctc agcacttcaa tctttccaag gctgaaaaca tcaatggtat
     3241 tgaaatactg gttcgggcca taccgcataa cggaattgct cagtcgtatt ccgtcgacca
     3301 taagcaatgt ctggttgcct gtcagtcccc tgatgaatgg cgatccaccg ccatgattcg
     3361 ttttctgtac aaaaactccc ggagtaagtg acagtgcttc aggaactgat cttgactggt
     3421 tttcccggat tgccgtattg tttaataccc ttacagcgtc aggcgtattg actaccctgg
     3481 aactgaaccg gcttgccgaa acaactactt ccgacagagc gggtgaaacc agtgtatcgg
     3541 gttcctggag tgttttgccc cctgcaatga taaaacccgg aagcatcagt aatgaaaccg
     3601 tcagaagcat tgacggcaaa ctacctatcc tggccccgtg tgaagccgta cgccaggtaa
     3661 tgcctgttcc gggaagaatg gaatctccat ttcttccgtg gtctgttttc tcaatattca
     3721 tcaaaatgaa gttgatgaac aaatcccggg cagatcttaa attaagtggc atggacttgt
     3781 ttatttaatt cattaattta ttcgacaaac ctgattccgt ttttctcccc gatgtttcgt
     3841 atgtaccctg cagccaacag atattcatcc tgggtttcaa gatgttccat cataaaagga
     3901 aggtccctga atttcactgc ttcgcccagg aacaccgaat aatcgagtgc acccagtccc
     3961 ggccttctct cttccaggtg aacagtgagc ctgggcagta ttattatatc ctttgcatgg
     4021 atcgacttaa gatagggtcc gagtttccgg aagcattcct tcaggaaggc agcgttgtta
     4081 aaataccttt gcgggctgga gatcatgttc acgggatcga gatgtgcggc aaattgcttc
     4141 ctgtcaattg ctttaatcag gtcgaggtag gaatccggcg aatcgggaag catgtaaggc
     4201 atcggctcaa gggtgtaaaa ggtgtttacc ggttttacct ggtcgatgat tgaccttata
     4261 ttctcaacaa tcaggtcaaa ggtatctttc gcataattgc ccgggtacgg gccatcccat
     4321 atttcacccc tggcccctga aatattgacg cagcatacag ctcctatccg gtcggccaga
     4381 tggagcgctt ccatgttctt ttccaggttt tcctttcttg tcttttcatc atcgtgaagc
     4441 atgttgttcc agaccccgac ctctgcaata atgatgtttt ttctcctggc ctcggcccgg
     4501 tatgccctta tcagatcctc aggggcaccg ggctgcaacg ggcaataagc cgccgagtac
     4561 cccagctttt tcactgccgc ggcccaatct gcaggatcac tgaactctcc ggggacaggg
     4621 cctcccagtc tgacagggaa atcagcctgg ctccccggca aagcttttat tgaccggaag
     4681 gacattgccg ccatgcctgc agccataatg gctccttttt ttatgaattt acgcctgttg
     4741 gtattactct tcatctcaga tatgcctttt caataatatc tgccaatata tttattctca
     4801 acaatcagct ttttcatttt taagggatcg attacgtcct gtttcttata aactattttc
     4861 cctccgggtt cgataagcat ggtgaaaggc agggcaccct gccagtcggg gtccaccgct
     4921 tcgatggcct tgtaaatatc gtcatcgttg aatatataat tcctgttgga agcttcattc
     4981 ttctgaagga acctgagtgc atccgccttt ctggcctttt tatctgcact aacggttatg
     5041 aattcaaatt gtctgcctct gtacatcctg tcgataatga ccagatccgg gaactcggtt
     5101 atgcagggtc cgcaccatgt ggcccagaaa tttatcagtc tgagcttacc ggagcctgag
     5161 ttccttacaa gttccttgag ccctgcaaca tcaatttctt caatggttac aggcaattca
     5221 gcccattgtt tatacagtct cctggtataa tcatcgttcc atgcccattt aatcgaacag
     5281 ccgaaggttt ttgtcacggg tgtgcctacc ggtctgccgg ccagcacatc gtctatggcc
     5341 ttcctgagat cttcgcctcc ggcagagccc ggcttttctg atccgtcgat gcggccgttg
     5401 tatctcagga tcctgtccct gtcaaagaca aaacaatgcg gagtggccac agggccgtaa
     5461 gccagcgcac atttctgatc atctccgtcg tacagatatg gaaaattaaa acccttatcc
     5521 ccggcccggt atttcatatc ctcaaaacta tcccccacat cagtataacc catttccgag
     5581 tagctcagag tcctgacaga gtttgaggag atcatcacca aagcaacacc cctggacttg
     5641 taatcatttg tgatggaaat aatcctgtct tcataagcct gggctgtcgg acaatgatta
     5701 gcgctgaaaa taatgaccag caccggagcc ttgctgaaat ccctaagggt ccaggtcttt
     5761 ccgtcaatgc ccggaaggct gaaatcaggt gctttggaac caattgcaag ggtttttggt
     5821 tcaggatgat ctgcctgcgg caaagctgta tggaaaaatc ccgtgacaat tatgatcagc
     5881 acacttattc ttagcatttt attcatatcc gcgaattttt cattattata tattcaaata
     5941 tatcttcttt ttcctttttc accggtaaac gggtcatttt tccagagcaa tttctgctaa
     6001 ttttggtttt aaaacaaaaa tccgtcagtg atggccagca ggaaacctgt agcaggattt
     6061 atcggagcag gcggtattgc acattcacat gcctatgctc tcgatgcgat gaaacattat
     6121 taccatgacg caccggaaat cgaaaaagag gctgtaagtt cggccactag ctcaggcagg
     6181 gagtcctttg caaacagata tggtttcaga tcttctcttt gccccgggga tttctttgaa
     6241 aacgggagaa taaactcagt atttatcctg ggacccaaca acactcatat attgagcatt
     6301 tccggaaagc catgatgatg ccttcggttg aacgtattta catcgaaaag ccattatgcg
     6361 tgagttctga tgaagaatca gtaattcgcc ggatcgcgca ggagcgcgcg agcataaaaa
     6421 tccaggcagg atatcagttc cttttttcat ctgccgtcag agaagctctt cgtttctgga
     6481 gatcaggagt ccttggcaag cctgttcact ttgatttcag atattatcac ggcgactatc
     6541 ttgcaaggga ttaccgtaac cgaaggagaa caaggcttat gcctgctccc gaaggtggag
     6601 caatggcaga cctgggctct catataataa gcatggccat agccttcatc gggaacaata
     6661 tggacgtcac aagcgccgtc caggcgggaa gtttcgatga tgtgccggct gattccgatc
     6721 tcttcagcct gatcaccctc catgatccgg aatcaggggc agccggcgtt atttcggcaa
     6781 gcagggtaag ttcaggcacg ggggatatgc tgtcatttga gcttcatgcg gaaaaaggtt
     6841 ctcttaaata ttcatcgcat ttccccgatt tttttgaata ctacaccgag gacagcggaa
     6901 tatggcataa aatttctacc ggaagcagtt ataaaccggt cagcagtttt ccgtcgggtc
     6961 atgtacctcc tggctggctg agggccctgg tccatgccca ctatgttttt ttcacatcgg
     7021 gtgaaagcga atcaatgctt ccggacctgg aacacggatt ggaggttgag cggatcctga
     7081 gagagagtgc cgggcaaatg cgcatcttcc gcaatagggt ctgcagataa catttcgttc
     7141 aatatgccga ttcggaaatt aatgctttta attcccgcat ggaggttttt tttgattatt
     7201 aatttatcct gcataccttt aaccttatat gatctgaatc caatgattat ttttctattg
     7261 tttaaccata ctgaaaaata tggcagcttt tattgatgaa ttcatgaaga catatggtcc
     7321 ggaagtgacc aggcagatgt ctgcaagctt tgatctcgat caggatactg ttcagaagct
     7381 gatcccgcag ctggctccac tgatccttgg cggactgaag cggcagaagg acgaccgtgg
     7441 cggtgacgcc agggttgatc atatacttaa taaatatggt gaccagtcgg ttcttgacaa
     7501 catcagggac ctgatggcca gtaaagcatc agcttcatct gttgatgcca atcttggagg
     7561 attgctcgga agcatggggg gcgtacaggc cgcccaggta ctgggcaaat caatgaatat
     7621 agacgcttcc aagataatga agatgatacc cgcacttgcc cccctgatac tcggggctct
     7681 cacccgcagg cgtgacacag ggggcggagg tatttctggc ataggatctc ttctggatgc
     7741 aaatggcgat ggcagtatca ttgatgatgt tgcagggttt ttaatcaaag gtacagcatc
     7801 acagggcaag ggcaagggaa tccttggatc gctgctggga ggaatgctcg gaaccaaatg
     7861 accaacatca ttgcaagtct tattacagca aacttcctgc tgccaaacct cctgtcttaa
     7921 actgttttca acagtttttc cagagttaac actctgagtg caataattca atacgacata
     7981 acccatatga ttgaccaata atacaatcac gaataaaatt aattgtgttc aatcatattt
     8041 ggttccctgc ccgaggggtc agactattat ccgaccttaa aaaccagatt aaaaacgtat
     8101 gaaatccaat ttaagaattg accatggtta gttcaggtaa aatcaaccgg agaaaatttc
     8161 tcggatctgc tgctgccgcc tccctggctt ttacaattgt gccgagaaat gttatcggag
     8221 gcaagggcca tgttgcacca agtgacaaac ttacccttgc ctatataggc tgcggaaccc
     8281 agggactgag agaaatggtt accctgattc agaatcccga cattcagatc gtaacagtat
     8341 gcgatccgaa caagtttacc accgactacg tcgactggtc gcatgacagt atcagaaaca
     8401 gtatccggaa agctcttcag gacgcttcct ggggtgaaaa aatcaaaggt attcccgggg
     8461 gcagggatat aggccaggaa ttcgtacaaa aatattatgc caaaatcagg aatctttccg
     8521 gatataaatg cccatcctat tctgatttca gggaattgct tgaaaaagaa aaagacatca
     8581 gttccataaa gattatgact cccgatcacc ttcatgctgc cattgccatt gcatcgatga
     8641 aaaaagggaa acatgtcgta acgcacaaac ccatagcaaa caggatgaat gaagcaaggc
     8701 ttacgatcga gaccgcaaaa aagtcaggac tcagcacaca tctcctggcc tggagcaggc
     8761 ggccggaata tgaaataatc agggattgga taaaggatgg ttttataggt accctgaaag
     8821 aaatacacaa ctggtcctat cgtccggtat ggccgcagtg gcagtcaaat ccgaccgaca
     8881 caccaccggt cccgaaaggt ttcgactggg cgttgtggct cggacccgtt ccggatcgtc
     8941 cctaccaccc caattataca cataatgtgt ttcgaggctg gtacgatttc ggaggaggaa
     9001 gcatcgctga tatgggccat tacagcctgt tcccgctgtt tatcaccctt ggcataaata
     9061 ccccggcaaa aactgcagaa gcttacggaa caaccaccag gattattaaa gacaatgttt
     9121 ctacagccgt ggtaaacgat gtggcttttc ccctttcctg catgatccgt tttgagtttc
     9181 ccgcccaacc cgctctgccg ccatttgatc tcatatggta tgatggcggg atcaagccgt
     9241 tggttcccga tgagctcagg gcagcaggga aaacactggc tacggaagga atgatgttcg
     9301 ttggcgacag gggaaagatt cttgcaggct tcaggggcga aaaccccatt ctgctcccgg
     9361 agtcaaaaat gaactatctg aaaaaacaag aagaggacga atcagaacgc cgaagcgata
     9421 aaatatggat tgatgcattc aaaaatggaa cgcagcccga tggaagcttt atttatgcag
     9481 gtccggtcac tgaaacgatt ctccttggcg ccgtggctct cagagcagga aaaaaagtcg
     9541 agtatgaccc tgtgaacatg aagattacca atgttcccga agctaacaaa taccttacca
     9601 gggaataccg gcccggatgg gagctttaaa aaagtatact gttattcttt taacaataaa
     9661 ccactcataa aattatcatt atgaatgaaa tctcacgaag aagcttcctt gaaaaatcaa
     9721 ttgtcgcggg agcaactgcc atgattacac cgtcggtgct gaaatcagct gctccggccc
     9781 tcacaccccg aatgacccgg gatgacattt cgctggcaca atgggccctg gtcgaggaga
     9841 taagagccgg aaaatggaaa acacttgatt ttccgtctgt tgcccgaaag gatttcggac
     9901 ttaacggcat tgaatttgtg aatacacttt tcgaggttcc gacagaggga tacctcaggc
     9961 gtctcaaaaa gaatgcagag gacaacaatg tcacgatggt cctgatcatg gttgacgatg
    10021 aaggtgatgg ctgttcaccg accagggagg gacggaaaca gtttgatatc aatcatcgca
    10081 agtggatcga cattgctcat ttcctgggat gttctgccat aaggacaaat tgcagggggc
    10141 ctaaggatgt aaacaaggat gaagccctga aatactcggt tgagtcttac cagatgttgc
    10201 ttgaatatgc agttcctgca aaaataagtg tcctgataga aaatcatgga ggaatatcag
    10261 acgatgctga ctggatggta tctctcttta aggcggtaaa taacctctat ttcggc
//
LOCUS       DAOOYG010000092        10264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_7042_length_10264_cov_5.911940, whole genome shotgun sequence.
ACCESSION   DAOOYG010000092 DAOOYG010000000
VERSION     DAOOYG010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7042_length_10264_cov_5.911940"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1264
                     /locus_tag="PK288_08340"
     CDS             44..1264
                     /locus_tag="PK288_08340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPM18973.1"
                     /translation="MNRVVITGMGIYSSIGKNLVEVNDSLYRGKSGIILDPARKAFGY
                     QSALTGKLDRPVLKGLIDRRSRVCMPEHAEYAYISTIEALKTAGLDNDYLEQNEVGIL
                     FGNDSSMYPVVECVDIVRDKKDSTLVGSGSVFQSMNSTVTMNLSVIFKLRGINFTLSG
                     ACASGSHSIGMGYFLIKHGYQNTIICGGAQEANIYCVVSFDGLGAFSKQETEPEKASR
                     PFDKRRDGLVPSGGAATVILENLDHALKRGAPILGEVTGYGFSSDGVHISVPDVDGPV
                     RAIMMALKDAGIQPGDVDYINAHATSTPLGDQNEAKAIDRVFGKNKPPVSSTKSMTGH
                     EMWMAGASEIVYSLLMMQNSYIAPNINLEEPDEYSAALNIVRQTTPMEFNTFLSNSFG
                     FGGTNSCLVIRKFH"
     gene            1288..1536
                     /locus_tag="PK288_08345"
     CDS             1288..1536
                     /locus_tag="PK288_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPM18974.1"
                     /translation="MTREEIIGIINNLLIEEFEISEAQLMPGAHLKNDLGLESLDFVD
                     IAVIVQKEFGLTLKGEEMTTITTLQSLYDHIANRYNKN"
     gene            1576..2442
                     /locus_tag="PK288_08350"
     CDS             1576..2442
                     /locus_tag="PK288_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPM18975.1"
                     /translation="MAGYKIFVTVLKYLGLSFAYFILYFVAFYFLFASRQSFVNTYRF
                     YRFRLGFGSVRSVLCVYRNYYTFGQVLLDKTALLAGFENKFTFTFDGEDHLRNMVESG
                     TGGLLVSAHVGNFEMAGNMLERLDTQVSIIMVDAEHEKIRDYLSTLTRKSFNIIAIRE
                     DNSHIREIGRAFEEKQIVCIHGDRFVKGSKIIPASFLGERAFFPGGPFYLAMKFNVPL
                     SFVFAMKEPGKHYHFYASPPKIFSLPGSQQKRDLVITQILKEYICQLETMTGRYPLQW
                     FNYYNFWAPYEQ"
     gene            2432..3790
                     /locus_tag="PK288_08355"
     CDS             2432..3790
                     /locus_tag="PK288_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HPM18976.1"
                     /translation="MSNRRLLLVSANRFTVPYPVYPLGISYLKAYLNETMPSLKIDVF
                     DFMTGSFEDYIKLLHEIKPDFTGISLRNIDDVNIYRQESFISHYRQIIKSTRENSSSV
                     IVIGGSGFSIYPELLFKTLEPDFAVYGEGEISLEKLLSAIENNLDYSTIEGLLYREHD
                     RIVFNERKTFFNTPTLSFDSGMLDYYWQNSGMLNIQTKRGCPYNCIYCTYPLIEGHRV
                     RTIDPGRIVKTLTDLKHRFNINYIFFTDSVFNISNSFNYRLADKMIEAELDIRWGGYF
                     NFANIDFKLLEKLRQAGLKHIEFGTESLSDRMLENYRKPFTVSEVLDISSACNDLEID
                     FAHFLILGGYGETESTLAETFENSKKINRTVFFPFIGMRIYPGTRLHEIAIAEKAVKP
                     DDPLLEPVYYVSREIDIDTLKEKAKRTQKQWIFPDEDMSSAMMRMRKRNRKGPLWEYL
                     LK"
     gene            3800..4240
                     /locus_tag="PK288_08360"
     CDS             3800..4240
                     /locus_tag="PK288_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HPM18977.1"
                     /translation="MEPSDYDILDLIPQRRPMLMIDRLVHAGEKYGEGVFRVRESNPL
                     CLGKHLSEAGILECIAQTAAAYTGYLRLSAKKDVVRGYIGSVKNLKIHFLPRAGDEIR
                     VGITLQDEVLGFTIVTGRIECLGNLVAECEIRILLEEHDIIPSG"
     gene            4306..4731
                     /locus_tag="PK288_08365"
     CDS             4306..4731
                     /locus_tag="PK288_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18978.1"
                     /translation="MKVSEVPQDKGYLIEGRISDLNYAIDNDGRYTSRQSRGWKPKNE
                     AIKFAWDQVYEHAGKLRQQILDGILSPIALYMELNLMDISILAGYTGISKRKVRKHLR
                     MKEFLKLPKEMIDRYAEALNITAEDLVNIGKIREFKYED"
     gene            4721..5725
                     /locus_tag="PK288_08370"
     CDS             4721..5725
                     /locus_tag="PK288_08370"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HPM18979.1"
                     /translation="MKIEFEHRQAAHCENGVTAGLLRYYGIDLSEPMVFGTGSGIFFS
                     HMVFIRVNEMAVTSFRVWPGVIFKRVTKRFGVSIKWRKFRDPDKAMQALDSLLARGIP
                     VGMLVGVFNLPYFPKEYRFHFNAHNITVIGKEGDKYLISDPVTENVESLTYDELKKVR
                     FAKGTVPPKGRMYYVTSMPEKFDLKSAIIKGIRKTANDMINIPLPMFGVKGIRYLAGK
                     MREWPSKLGDRSAVMNLGQVIRMLEEIGTGGAGFRFIYAAFLQESARVLQLPVLDELS
                     VEMTGAGDRWREFAWQASRCFKNRDNGEITYDMLADILLDIAAREERVFRQLGQLKFT
                     "
     gene            5722..6474
                     /locus_tag="PK288_08375"
     CDS             5722..6474
                     /locus_tag="PK288_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM18980.1"
                     /translation="MTIRTAIEIKNLTKRYRGNPQPAVNGVSLDIEEGHFFGLLGPNG
                     AGKTTMIKIMCGLLSPSSGEVTIGGLSLRKQLPEIKRSIGIVPQDIALYSTLTARENL
                     KIFGGLYGIPARKLKERIDKWFSIFGLEKNREQTIGSYSGGMKRRLNLIAGILHEPKI
                     LFLDEPTVGIDVQSKKVITENLREINQGGTTIVYTSHYLEEAESLCSSIAIIDQGVII
                     IKGTMEEIRSETNNMSKLEDIFLQLTGMEPRD"
     gene            6476..7753
                     /locus_tag="PK288_08380"
     CDS             6476..7753
                     /locus_tag="PK288_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPM18981.1"
                     /translation="MKSIRTLLYKDFLILIRDKWGLGLLFAMPMALVLIMTAMQDTTF
                     RSLYETGINLVLLNNDLDSLGNAVEKEISGSGIFNCNTSINGHRPIESEIREAVAAGR
                     YQIGIIIPANTTSRIRERVKTSMENIFSGSGVNPGKTDSIYITLLLDPAIRSSLRTYI
                     QSKISEYASKIETRFFLNELTLEINRQMIVSVANLNLLQPQTVTCREELVAFGNRIAI
                     PNTAQHNVPSWTLFAMFFIVIPFAGAMIKEREDGNLERLLAMPASYGSIMISRVLVYL
                     VVCFLQFVLIMAMGVYLFPLLKLPSLVIAGRMPDLAAMAVFASLAAVSYGIAIGTMAH
                     THQQAAIFGSVSVMILAALGGIWVPVFIMPPFLKSLSVISPMNWALNGFYDILVRNAS
                     LSGVIHYGVRLVLFSLAAILAALYFNRIRKGLT"
     gene            7893..8156
                     /locus_tag="PK288_08385"
     CDS             7893..8156
                     /locus_tag="PK288_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPM18982.1"
                     /translation="MEDLIAQIKQDIITSLSLEDVRIDDFNADTDLFVTGLGLDSIDA
                     IELILLMEKKYGVKIEDPRERRTVLRNVRTMAECILNHRNTIY"
     gene            8156..9346
                     /locus_tag="PK288_08390"
     CDS             8156..9346
                     /locus_tag="PK288_08390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPM18983.1"
                     /translation="MQPGVVVTGIGIVSAIGIGTEETLTSLLNKRSGIAPVRWLETIH
                     SGIPVAEVSHSTDELAEIAGTSGQPGIFTRNTLLAMIAAGQSVEMSEPSGSGLDQAGL
                     VMATTVGGMDYNEKHYRSLLAGKETAGGTSVLDSSDTTEKLAARFGILRNVTTISTAC
                     SSSANAIMLASRLIRNGKLTRVLAGGSDALTIFTLNGFRGLELLSPTGCRPFDAHRDG
                     LTLGEGAAVLMLESDRTADMSKALCRISGYANVNEAFHQTSASPDGAGAFMAMSECLE
                     SAGLEPWEIGYINAHGTGTEMNDRAEALAIERLFGRKIPPVSSTKAFTGHTLGAAGAI
                     EAVISVLAIREQKVWPSIHINEVMPEISFLPPNDTLSVPVGHVMSNSFGFGGNNTSLI
                     FSEI"
     gene            9343..>10264
                     /locus_tag="PK288_08395"
     CDS             9343..>10264
                     /locus_tag="PK288_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPM18984.1"
                     /translation="MKAFIRSAGIISPQQTYDCRGFPEVFRGGISDRLSCIEPDYHSL
                     INPVQLRRMPRILKMGLAAARLALSDQENADPDGIIVGTGLGCLRSLETFLDEIFDTG
                     EQIKSVLPFINSTHNAVAAQIAMLLKNNSYNVTYCHRSFSFESALADALMQLKEKSGM
                     NILVGGIDELTDDFITLHRPERFRKEQLNTLELVSAPDRGTIPGEGAAFFLISDNSGN
                     GACAGIRGVHTFLTDPRSAQELIFAETDKFLESSGMDRKDIDLVILGMNGDTESDAIY
                     KELARDYFDSSTGIGCYKHLCGEYYTSTAFA"
BASE COUNT         2901 a         2291 c         2473 g         2599 t
ORIGIN      
        1 ctgtatacat aatgaaataa gaatatcttc cattgtcgta cagatgaaca gagtagttat
       61 aaccggcatg gggatttact cctcgattgg aaagaacctc gtggaagtaa atgattctct
      121 ttacaggggc aaatcgggaa tcatcctgga tccggcaaga aaagctttcg gctatcaatc
      181 ggccctgacg gggaaacttg acaggccggt actgaaagga cttattgaca ggagatcccg
      241 cgtctgcatg cccgaacatg ccgaatatgc gtatatatca accattgaag ccctgaaaac
      301 agccgggcta gacaatgact accttgaaca gaacgaagtg ggtatcctgt tcggcaatga
      361 cagctcaatg tatcctgtag tagaatgtgt tgacatagta cgcgacaaga aggactccac
      421 gcttgtcggt tcaggctcag tgtttcagtc aatgaattca acggtgacaa tgaacctttc
      481 cgttattttc aaactcaggg ggattaattt caccctgagc ggtgcgtgtg caagtggctc
      541 acattcaatt gggatgggtt acttcctcat aaagcacgga tatcagaata ccattatatg
      601 cggaggagca caggaggcga atatttattg cgtggtcagc ttcgacgggc tgggggcatt
      661 ctcaaaacag gaaaccgaac ctgaaaaagc ttccaggccc ttcgataaga gaagagacgg
      721 actggttcca agcggaggtg cggcaaccgt catccttgaa aatctcgacc atgccctcaa
      781 aagaggtgca cccatcctgg gcgaagtgac gggctatggc ttctcctccg acggggtgca
      841 catttccgtc cctgatgttg acggcccggt gagagccatc atgatggcac tgaaagatgc
      901 aggaattcaa cccggcgatg tggattacat taatgctcac gctacctcca cacccctggg
      961 tgaccagaat gaagccaagg caatagacag ggttttcggt aaaaacaagc cccccgtatc
     1021 ttccaccaaa tcaatgaccg gacatgagat gtggatggca ggagcaagtg aaatcgtata
     1081 ttcactgctg atgatgcaga actcatatat cgccccgaac atcaacctgg aagaacctga
     1141 tgaatactct gccgcactga atatcgtcag gcaaaccaca ccaatggaat ttaatacctt
     1201 tctttccaac tcattcggct tcggtggaac aaattcatgt ttagtaatca ggaaatttca
     1261 ttaaatttgc ccaagttttt tttaatcatg accagagaag aaattatagg gataataaat
     1321 aacctgctta ttgaagaatt tgagatttct gaagcccagc tcatgcccgg cgcccatctg
     1381 aaaaatgatc ttggacttga aagcctcgat tttgttgata tcgcggtaat agtgcagaag
     1441 gaattcggac ttacccttaa gggagaggaa atgacaacaa tcacaacact tcaaagtctt
     1501 tacgatcaca tagcaaaccg ttacaacaag aattaacctg atgtgactga ctggaaagga
     1561 aaaacccgcg gggggttggc aggatataag atattcgtca cagttttaaa atacctcgga
     1621 ctttctttcg catattttat tctttatttc gttgcttttt atttcctttt tgcatcgcgg
     1681 caatcattcg ttaatacata ccgcttctac cgcttcaggc tcggctttgg atctgtaaga
     1741 tcggttctat gtgtttacag gaactactat actttcggac aggttctgct cgacaaaaca
     1801 gccctccttg ccggtttcga aaataagttc accttcacct ttgatggcga ggatcacctt
     1861 agaaacatgg ttgagtcagg taccggcgga ctgctggtaa gcgcacatgt cgggaatttt
     1921 gaaatggccg gcaatatgct tgaaagactt gatacacaag tgagtataat catggtcgac
     1981 gcggaacatg agaaaatccg ggattatctt tcaaccctta cccgtaagag tttcaatata
     2041 attgcaataa gggaggataa ttcgcatatc cgcgagattg gcagggcctt tgaagaaaag
     2101 cagatcgttt gcattcatgg cgacaggttc gtaaaaggca gcaagatcat tcccgcctca
     2161 ttcctgggcg aacgggcatt cttccccggt ggcccgttct acctggccat gaaattcaat
     2221 gtcccgttgt cgtttgtttt tgcaatgaag gaaccgggaa aacactatca tttttatgca
     2281 agccctccaa aaatcttcag tctgccggga agccagcaaa aaagggacct ggtaataacg
     2341 caaatattaa aagagtatat ttgtcagctg gaaacaatga caggcaggta tcctttgcaa
     2401 tggttcaatt attacaattt ctgggctcca tatgagcaat agaagattat tgctggtttc
     2461 agcaaacagg ttcacggtgc catacccggt ataccctctc gggatctcat acctcaaggc
     2521 atacctgaat gagacgatgc cgtcactgaa gattgatgta ttcgatttta tgacagggtc
     2581 atttgaagat tacataaaac tgcttcatga aataaaacct gattttacgg gaatatcgct
     2641 gcggaacata gatgatgtga atatttacag acaggaaagt tttatttcac attacaggca
     2701 gataataaaa agcacaaggg aaaacagcag ttcagttata gtaatcggag gttcgggatt
     2761 ttcaatttat ccggaattgc tttttaagac cctggaacct gatttcgcag tatacggtga
     2821 gggggaaatc agtcttgaaa aattgctgtc cgccattgaa aacaacctcg actattccac
     2881 catcgaagga ctgctttaca gggaacatga caggatagtt ttcaacgaac gcaagacctt
     2941 cttcaatact cccaccctga gttttgacag tggcatgctc gactattact ggcagaacag
     3001 cggcatgctg aatatccaga cgaaaagagg ctgtccttat aactgcattt attgcacata
     3061 tccgcttata gaaggacaca gggtgagaac gatcgatccc ggccgtattg tgaaaacatt
     3121 aacagatctg aaacacaggt tcaatataaa ctacatcttt tttaccgact cagtcttcaa
     3181 tataagcaac agcttcaact acaggctggc agataaaatg atcgaagctg aacttgacat
     3241 ccggtgggga ggctatttca attttgcaaa catcgacttt aagcttctgg agaaactccg
     3301 gcaggccggt ctgaagcata ttgaatttgg aacagaatca ttgtctgacc ggatgctgga
     3361 aaattacagg aagcccttta cagtatccga ggtccttgat atttcctcag cctgcaacga
     3421 ccttgagatc gattttgctc attttctcat tcttggaggt tacggagaaa cggaatctac
     3481 cctggcagag acctttgaaa attcaaaaaa aatcaaccgg acagtcttct ttccctttat
     3541 cggtatgagg atctatcccg gaacccggtt acacgaaatt gctatcgctg aaaaagcagt
     3601 taaacccgac gatcctttgc ttgaacctgt atattatgtg tcacgggaga tagatattga
     3661 tacgttgaag gagaaggcaa agcgcaccca gaaacagtgg atttttcccg acgaggacat
     3721 gtcgtccgca atgatgcgga tgagaaaaag aaacaggaaa gggcctctct gggagtatct
     3781 gttaaaataa tcctgaatca tggagccatc tgattacgat atactggatc ttattcccca
     3841 gcgccgtccc atgctgatga tcgacagact tgtgcatgca ggggaaaaat acggggaagg
     3901 tgtttttcgt gtcagggaat cgaatccgct ttgtctcggg aaacacctgt cagaggccgg
     3961 gatccttgag tgcatcgccc agactgctgc tgcatacacc ggttacttaa gattgtcagc
     4021 aaaaaaggat gttgtcaggg gttatatagg atcggtaaag aaccttaaaa tccatttttt
     4081 gccccgtgca ggcgatgaaa tcagggttgg aataaccctc caggacgagg tgcttgggtt
     4141 caccattgta accggcagga ttgagtgtct cgggaatctg gtggccgaat gcgaaatcag
     4201 gatccttctg gaggagcacg atattatccc gtcgggttga acggataatg ttacactaaa
     4261 ctgatatcga atccttatat ttgcaacttt atttactctt aagtgatgaa ggtaagtgag
     4321 gttccccagg ataagggcta cctgattgaa ggcaggataa gcgatctgaa ttatgccatc
     4381 gacaatgacg ggagatatac cagccggcaa agccggggct ggaaacccaa gaatgaagct
     4441 attaaattcg catgggacca ggtttatgaa catgccggca aattgcggca gcagatcctt
     4501 gacggaatcc tgagccccat agccctgtac atggagctta acctgatgga tatttcaatc
     4561 ctggcaggtt ataccggtat ctcgaaaaga aaagtaagaa aacatctcag gatgaaagag
     4621 tttcttaaac tgccgaaaga aatgatcgat cgttatgccg aagctcttaa tattaccgct
     4681 gaggatcttg tcaacatcgg aaagatcagg gaattcaaat atgaagattg agtttgaaca
     4741 caggcaggcg gctcattgcg agaacggggt aacagcaggt ctgctaaggt actacggtat
     4801 tgacctgagt gagccaatgg tttttgggac cggatcgggc attttcttct cccacatggt
     4861 attcatcagg gtcaacgaaa tggctgtgac ttcctttcgc gtctggcccg gggtaatttt
     4921 caaaagggtt accaaacgat tcggggtcag tataaaatgg cggaaatttc gcgatcccga
     4981 caaggcaatg caggcgcttg acagtttgct tgcacggggt attcctgtag gaatgcttgt
     5041 cggcgtattc aatcttcctt attttccaaa ggaatatagg ttccatttca acgcgcataa
     5101 tatcactgtt attggaaagg agggtgataa atatcttatc agcgatcccg taaccgaaaa
     5161 cgttgagagc cttacatatg atgaactcaa aaaagtgagg ttcgcaaagg gaacggttcc
     5221 cccgaagggc agaatgtact atgttacttc aatgccggag aaattcgatt taaaatctgc
     5281 gataataaag ggaatccgaa aaacagccaa tgacatgatc aacattcccc tgccaatgtt
     5341 tggcgtgaag ggtatacgtt acctggcagg aaaaatgcgg gaatggccct caaaactcgg
     5401 agacaggtca gctgtaatga acctgggaca ggttatacgg atgcttgagg aaattggcac
     5461 gggaggtgcc ggattcaggt tcatttatgc cgcctttctc caggaaagcg ccagggtatt
     5521 gcaactgccc gtacttgatg aactcagtgt cgaaatgacc ggtgccggcg acaggtggcg
     5581 ggaatttgcc tggcaggctt cccgctgttt caagaatcgc gacaacgggg aaattacata
     5641 cgatatgctg gcagacatac ttttggacat tgcagcaagg gaagaaaggg tcttcaggca
     5701 gctcggacaa ttaaaattca catgaccatc cggaccgcaa tcgaaataaa aaacctgaca
     5761 aaaagataca ggggaaatcc ccaacctgct gtaaacgggg tgagtcttga tatagaagaa
     5821 ggccatttct tcggactgct tggtccaaac ggtgcaggca agaccaccat gataaagatc
     5881 atgtgcggac tgctttcacc ttcctccggg gaagtgacca taggaggctt atcactcagg
     5941 aaacagctgc ctgaaattaa aagatcaata gggattgtac cccaggacat cgctttgtat
     6001 tcaactctta ctgccaggga aaatctgaaa atctttggcg gattatacgg gattcctgca
     6061 aggaaactga aagagcgcat cgataagtgg ttcagcatct ttgggcttga aaaaaaccgg
     6121 gaacaaacaa tcgggtcata ctccggaggt atgaaacgca ggcttaatct tattgccggt
     6181 attcttcatg aaccgaagat ccttttcctc gatgaaccta cagtaggaat agacgtacag
     6241 tcaaaaaaag tcataaccga aaacctcagg gaaatcaatc agggaggtac aacaatcgtg
     6301 tatacctccc attacctcga ggaagcggaa agtctttgct cttccattgc catcattgat
     6361 cagggtgtga taataataaa aggcacaatg gaggagatca gatcggaaac caacaatatg
     6421 tcaaaactgg aggatatctt tcttcagctt acaggtatgg agccaagaga ctgaaatgaa
     6481 aagcatccgg acattactgt ataaggactt tctcatcctg attcgcgaca aatggggact
     6541 gggactatta ttcgccatgc caatggcatt ggtccttatc atgactgcca tgcaggatac
     6601 cacattcagg tcactgtacg agacagggat caacctggtc cttttgaata atgatctcga
     6661 ttccctgggc aatgcggttg agaaagagat ttcaggatcg gggattttta actgtaacac
     6721 ctcaataaac ggccatagac ctatagagtc agaaatacgt gaagccgttg ctgccggaag
     6781 gtaccagata gggatcataa tcccggctaa cacaacttca aggattaggg aacgtgtaaa
     6841 aaccagtatg gaaaacatct tctcgggctc tggcgtcaat cccggaaaaa ccgactccat
     6901 ttacattacc ctcctcctgg atccggcaat ccggtcatcg ctccgtacct acatccagag
     6961 taagatcagt gaatatgcat caaagatcga gaccaggttt ttcctgaatg aactgaccct
     7021 ggaaattaac aggcagatga ttgtttcggt tgcaaacctt aatcttttgc agccccagac
     7081 ggtaacctgc agggaggaac tggttgcttt cggcaaccgg atcgcgatcc ctaacacggc
     7141 tcagcataat gttccttcat ggacattgtt tgcaatgttt ttcatagtga tcccgtttgc
     7201 cggggcaatg attaaggagc gggaggacgg aaatctcgaa agactgcttg caatgccggc
     7261 ctcctacggt tcaataatga tctccagggt gcttgtttat ctcgtggttt gtttcctgca
     7321 atttgttctg ataatggcca tgggggttta cctgtttcct cttctgaaac tcccgtcact
     7381 ggtcattgcc ggaagaatgc cggatcttgc agcaatggct gtttttgctt cccttgcagc
     7441 ggtaagttat ggaatagcaa taggcaccat ggcccacaca caccagcagg ccgcaatttt
     7501 cggatccgtt tccgtaatga ttctggctgc cctgggagga atatgggtgc cggtattcat
     7561 tatgcccccg tttctcaaga gtctgagtgt tatatcgcca atgaactggg cacttaacgg
     7621 cttctatgat atacttgttc gcaatgcatc gctttccggc gtgatccatt acggcgtcag
     7681 gctcgtttta ttttcgctcg cggctattct tgcagcatta tatttcaaca ggattagaaa
     7741 aggactcacc tgagaataca tgaattcccc ttcagtacag ggctgaactc aattaattat
     7801 gctgattgaa aagagatttt tataaattta caccctgatt tttgtttttc acggcacttt
     7861 cagtgtgcct cgcatacagg attcagattt ttatggaaga tttaattgca cagataaaac
     7921 aggatattat tacctcattg tcgcttgaag atgtcaggat tgatgatttc aatgccgaca
     7981 ccgatctctt tgttacgggt ctggggctgg attcaataga tgctattgag cttatccttc
     8041 tgatggagaa gaaatacggg gtaaagatcg aggatccgag ggaaaggcgt accgttctga
     8101 gaaatgtcag gacaatggca gagtgcatct taaaccacag aaacactatc tactaatgca
     8161 accgggggtt gtagtcacag gtatcggcat tgtatctgcg attggaatcg gcacagaaga
     8221 gaccctcaca tcattgttaa ataaaagatc agggatagcc ccggtccggt ggcttgaaac
     8281 cattcattcc ggcattcccg ttgctgaagt cagccattcc accgatgaac tggctgaaat
     8341 tgccggcaca tccgggcagc ccggtatttt tacccgcaac actttactgg cgatgattgc
     8401 tgcaggacag tcggttgaga tgtcggagcc atccggttcc ggactcgatc aggccggtct
     8461 tgtcatggcg actaccgttg gagggatgga ttacaacgag aagcactacc ggtcattgct
     8521 ggccggaaag gaaacagcag gcgggacatc cgtccttgac agttcggaca ccaccgaaaa
     8581 gctggccgcg cggtttggta tacttcgcaa tgtaacaaca atatcaaccg cctgctcctc
     8641 ctctgcaaat gcaataatgc ttgcttcgag gcttataaga aacgggaagc tgaccagagt
     8701 actggcaggt ggatctgatg ccctgaccat attcaccctc aacgggttca ggggactcga
     8761 attgctttcc ccgacgggat gccggccttt cgatgctcac cgcgacggac tgacactcgg
     8821 agaaggtgcc gccgttctca tgctcgaatc ggatcgtacg gcagatatgt caaaagcact
     8881 ctgcagaata tcgggatatg ccaatgtaaa tgaggcattt catcagacat cagcatcacc
     8941 tgacggagca ggggctttca tggccatgtc ggaatgcctc gaatcagcag gccttgaacc
     9001 ctgggaaata ggatacatca atgcccacgg taccggtact gaaatgaatg accgggccga
     9061 agcgcttgcc attgaacgtc tgttcggcag gaaaatacct cccgtaagct ccaccaaggc
     9121 attcaccggt cacaccctgg gtgctgcggg tgcaatcgaa gccgttatct ctgtcctggc
     9181 aatcagggaa cagaaagtct ggccctccat ccacattaat gaggtcatgc cggagatctc
     9241 ctttttaccg ccaaatgata cactttcagt gcctgtaggg catgtcatgt ccaattcatt
     9301 tggttttggc ggaaataata catccctgat attctcggaa atatgaaagc ttttatcaga
     9361 tctgccggaa taatttcgcc gcagcagact tatgactgca gaggatttcc agaggttttc
     9421 aggggaggta tttccgacag gctttcctgc attgaacccg actaccattc attaatcaat
     9481 cctgttcagc tgcgccgtat gccaaggatc ctgaaaatgg gactggcagc agcaaggttg
     9541 gctttatcgg atcaggaaaa tgccgatccg gatgggatca ttgtaggaac cggactgggt
     9601 tgcctgagaa gccttgaaac gttcctcgat gaaatctttg atacaggtga gcagatcaaa
     9661 agtgtgctgc cgttcattaa ctcgactcac aatgccgtgg ctgcacagat cgccatgctg
     9721 ctgaaaaaca acagttacaa cgtcacatat tgtcatcgca gcttttcctt tgaaagtgca
     9781 ctggcagacg ctcttatgca attaaaggaa aaaagcggca tgaatattct tgtcggggga
     9841 attgacgaac taaccgacga tttcataacg cttcacaggc cggaaagatt ccgtaaagaa
     9901 caattgaata ccctggagct tgtctctgca cccgataggg gtacaattcc gggagaaggt
     9961 gccgcttttt tcctgatctc ggataacagt ggaaacgggg catgtgcagg catcagaggc
    10021 gttcatacat ttctaacgga cccacggtcc gcacaggaac tgatctttgc tgaaactgac
    10081 aaattccttg agtcctccgg aatggaccgg aaggatatcg atcttgtaat cctgggaatg
    10141 aacggtgata cggagtctga tgctatttac aaagaacttg ccagggatta ctttgattca
    10201 tccaccggta tcggctgtta caagcatctg tgcggggagt attatacctc gacggccttt
    10261 gcgc
//
LOCUS       DAOOYG010000093        10244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_7075_length_10244_cov_6.747276, whole genome shotgun sequence.
ACCESSION   DAOOYG010000093 DAOOYG010000000
VERSION     DAOOYG010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7075_length_10244_cov_6.747276"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="PK288_08400"
     CDS             <1..1200
                     /locus_tag="PK288_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18985.1"
                     /translation="AMTVAFILYSFIGGLIASAYTDFIQSFLIITLSIILIPLGLREI
                     GGFSYMRSVLPEHHFDVFNAASGIDTFTILMLAINGIVGITAQPHMISMCATGKTERA
                     GRIGQTFGSMVKRLVTIGWALTGLVVGALAIKNSVTLPDPEMAFGYACGELLGPGLVG
                     LMLSAIVAANMSACSNFMVNIGALFTKNLFRKYFRPDATDRDLLWTGRYSGLALAFLA
                     VLFALSIKNVLHAFLFTETLAAFMGIMFLGGLIWKRANRYGAGAAVLSSLIVYYLANY
                     FYSGNLQLVYVWKPAPFGFAMFAGFLMFWLVSVLAPPEDKEKIELFFDNMRRKSDSAS
                     TDALGRKPLGSETGEDLLLLDLPGWRRKERWQNFFKRYREDLVGFLISWIVVGLLIFL
                     AWAILQI"
     gene            1336..1524
                     /gene="tatA"
                     /locus_tag="PK288_08405"
     CDS             1336..1524
                     /gene="tatA"
                     /locus_tag="PK288_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPM18986.1"
                     /translation="MGNFGITEIILVILVIVLLFGGRKIPELMKGIGQGMKEFKKASK
                     VEDDPEKTKTGSSENKGE"
     gene            1735..2658
                     /locus_tag="PK288_08410"
     CDS             1735..2658
                     /locus_tag="PK288_08410"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM18987.1"
                     /translation="MITRRKFFRNSALASAGLIAGAGSAGMACSNRDNPGSYDIMEEA
                     LKFRKINAHEHVYLGSEPSSLINAADRLGMEKLVVSLPVTVEDRVLVTPEKFRECNNL
                     VLKAMKLFPDRIMGQFYINPYYSKESLEEIDRCVSAGMVSMKGYYQVKINDPLYYPVI
                     EKMIDLKMIILMHTHCGIGVGGYRTKYGNIQANASTPDDFAGAAERYPEALFQYAHTG
                     GGGDWEYACKTLRDYPNVYVDTSGSNNESGMIEYAMKHLGEDRLFFGTDGSYYQGVGS
                     ILAANINDDQRKKIFFDNYNLILKRSGNHVD"
     gene            2648..3433
                     /locus_tag="PK288_08415"
     CDS             2648..3433
                     /locus_tag="PK288_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18988.1"
                     /translation="MLIDVSANIGHWPFRQTGLKTCRELLGNMDRYGVEISVISNMNG
                     IFYKDSHAANEELYREISSEKRFRGRFVPFAVINPLYAAWRHDLLVCSRELKFKGLRL
                     YPQYHDYEITDPACIDMVRCARDEGMVIALTVRMVDSRLRSWMDLSSEWILKDVIPLI
                     REVPDARYMILNVSGGIMLNDEDTALIRGTDMIFDTSGRNISDLGSLLKKYGNDKFAF
                     GTHSPILDYLTGLLRIEALADNEADESTREMLRSGNAKRLLGI"
     gene            3671..5836
                     /locus_tag="PK288_08420"
     CDS             3671..5836
                     /locus_tag="PK288_08420"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPM18989.1"
                     /translation="MKKNQIVAAASFIVIAFLSSPLRLTAQEEDLSVLSRWTEWSDAG
                     NMLIHHLNDHAFRYLDSRDRHVESLRTREDWTNRQNEVRNKLMEAVGPFPPKTPLNAR
                     ITGVLKKDGYRIEKLIYQSMPGFYVTGCLFIPDGIRGKRPAILNVIGHTDISFRGADY
                     QTLILHLVQKGFIVFAIDPIGQGERLQYFDGEKNVPVIGAGTAEHSYFGNQCFMAGAS
                     PARYFIWDGIRGIDYLVSRKEVDPGRIGVTGLSGGGTLTAYISAFDDRVKASAPSCYI
                     TGFRRLLESIGAQDAEQNFFHGIKSGITHADLIEVRAPKPTLIVSTTRDFFSIQGARE
                     SFGEAKKAFMAFGMSENLLMAEDHFGHGFTPRNNEATCAFFQKYLDMPGNPAPGVIRT
                     LNPDELKITATGQVSTSFDAETVFSLNAKEAEMLLANLENSRKDSEKHLAQVEKNAEL
                     LSGYNRYTEKPASVFRGTWQRDGYSVEMYAVGGNGKYVIPLLLFIPDGNGKYPALIYL
                     TPKGKAADAGEGGRIEQITRKGIIVAVPDLLGIGEVAPDESFFNASYFISVLTGKTLI
                     GTGAEDIDAIVNFLTERADVDHGRISSVAFDELSPVLMHAAVFNHSIGSIVLAGSQAS
                     FRSMVMNRFYDRKLAPYTVPGAIKYYDLPDLIGCIAPRKVVMAGIRNQMREYAGKELI
                     ETDLTFPVKVYTLKGKPDNFRILPPDEDILPMIDWCLAK"
     gene            complement(5917..7521)
                     /locus_tag="PK288_08425"
     CDS             complement(5917..7521)
                     /locus_tag="PK288_08425"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HPM18990.1"
                     /translation="MKIQHTGILHYTIILTILIMLPGCDQGKKPPTVNIDPEGRWLEE
                     MTIIMLQQGYKEGRYTVRDVVSAYLGRIDEIDRKGPGLNSVIAINPDAIQIAGELDAE
                     MKQGKIRGPMHGVPVLLKDNIDTHDKMPTTAGATALAGSIAPADSRVARKLREAGAVI
                     IGKANLSEWANFRASPSSSGWSGVGGQTKNPYVLDRNPCGSSSGSGAAVSANLCMVSI
                     GTETNGSIVCPSNNNGIVGIKPTVGLISRTGIIPISFTQDTPGPMARTVEDAAICLGI
                     LTGTDPSDSKTLLSEGHAHTDYRQFLSEDGLQGKRLGIIKWGSGFHEKVDTLMKRAIA
                     FMKTRGAEIIEVELPDGAGYEDASFQVLLYEFKDGLNKYLSGLGEGSRVKSLRELIEF
                     NKKDPVELRFFNQKILEMADKKGDLSSAEYRESLRKMLRAARDEGIDRVMNSAGLDAL
                     VAPTGSPAWKTDLILGDHSGGGSSSPAAISGYPAITVPMGFIDELPVGITFFGRAWSE
                     PVLLEIAYSYEQGTKHRSAPRFLATD"
     gene            7760..8833
                     /locus_tag="PK288_08430"
     CDS             7760..8833
                     /locus_tag="PK288_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HPM18991.1"
                     /translation="MNSIYIKIPENAGDISEYVLAQVIRTSGSTPRKSGSSALFNRYG
                     LVAGTIGGGVLEGKIQAVAMDPSITNKTGINEFILSNTAGGEDALCGGKASILIDSEL
                     QEHSDVFGALKDSYRARIPGVLITRILKNKKQETEISRYWLTEKTGSEVIGHFADSVR
                     NTAMEMLSLLKPDDFREVSLQASEENLSDTVYLESVIPPYRLLIAGAGHIGKALARIG
                     QMIGFEVTVADDRPEYANSDNIPWADHIVTGNIGRVVADTAKGKDLYIVIVTRGHRDD
                     AEALEACIGSDAAYIGMIGSRNKVELMRRDFIERKLATDEQWKRIYAPVGLDINSETV
                     EEIAVSIAAQIIQIRNSSQTLKP"
     gene            8854..9381
                     /locus_tag="PK288_08435"
     CDS             8854..9381
                     /locus_tag="PK288_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPM18992.1"
                     /translation="MVLSNSEIWAIILAAGESKRMKEPKMLLPVQGKTMIEKTITNVI
                     TSEVNNTLVVLGAFSNEIYDAISHLPVSVCINDKYVEGMLSSVQCGLCNIPGSAGAVL
                     FVPGDQPLIEPLVINRVINAYHESGKGIAMPVYEGKRGHPLLVSGKYMEEIKGFGTNE
                     SLGRLRAGMRMMCWR"
     gene            9470..9946
                     /locus_tag="PK288_08440"
     CDS             9470..9946
                     /locus_tag="PK288_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPM18993.1"
                     /translation="MEVKINFTLNGKKTTILTDPNQTLLWVLRNQLGLTGTKYGCGNG
                     FCGACTVLINKEPVRSCITPVTDVSGKEVITIEGLARNGKLNPVQKAFVEHDALQCGF
                     CTPGMIMTATGMLMKNPAITRKEIIEGLEENLCRCGAHGRIIDAVESAAKEMKGGK"
     gene            9949..>10244
                     /locus_tag="PK288_08445"
     CDS             9949..>10244
                     /locus_tag="PK288_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPM18994.1"
                     /translation="MKNKETKTAVRQEESTKNGLKRRNFVKLLGGGIFLFFRPWKGFG
                     GSGVSLSQQRRRLTTDYNAFLHITPTGIINCYTGKIEMGQGVITALVQMMADEL"
BASE COUNT         2782 a         2170 c         2559 g         2733 t
ORIGIN      
        1 gccatgactg tggcttttat cctctacagc ttcatcggag gcctgattgc gtcagcatac
       61 accgatttta tacagtcgtt tcttatcata accctttcta ttatccttat cccgctggga
      121 ttgagagaaa taggaggatt cagctatatg aggtcggtat tgcccgaaca tcatttcgat
      181 gtattcaatg cagccagcgg catcgatacc tttaccatac tgatgcttgc aattaacggt
      241 atagtgggaa taacagcaca gccacacatg atttccatgt gtgccacagg gaaaactgaa
      301 agggcaggca ggataggtca gactttcgga tcaatggtga aaagactggt tactatagga
      361 tgggccctta ccggcctggt tgtcggtgcg cttgcaatta agaacagtgt gaccctgccc
      421 gaccctgaaa tggctttcgg atatgcctgc ggtgaacttc tcggccctgg ccttgtcgga
      481 ctgatgctgt cggctattgt tgcagcgaat atgtcggcct gttcgaattt catggtcaac
      541 attggcgccc tgttcaccaa gaacctgttc cgtaaatatt tcaggccaga tgcaactgat
      601 cgtgatctgc tgtggaccgg ccgttattcg ggtctggccc ttgcctttct tgcagtattg
      661 ttcgctctct cgataaaaaa cgtcctccat gcatttctgt ttactgagac cctggcagca
      721 ttcatgggaa ttatgttcct tggcgggctg atctggaaac gggccaaccg ctacggggca
      781 ggagcagcgg tactttcatc attgatcgtg tattatctgg caaattattt ttattcaggg
      841 aatttgcagc tcgtgtatgt atggaaacct gccccgtttg gatttgcaat gtttgcgggt
      901 ttcctgatgt tctggcttgt cagcgtcctg gccccccctg aggataaaga aaaaattgaa
      961 ttgttcttcg ataatatgag aaggaaatcc gattccgctt cgacggatgc ccttggccgt
     1021 aagccccttg gttcggaaac cggcgaggac ctgctgcttc ttgatctgcc gggatggaga
     1081 aggaaggaac gctggcagaa tttcttcaaa agataccgtg aggatcttgt cggattcctc
     1141 atttcctgga ttgttgtcgg actgcttata tttcttgcat gggcaatcct tcagatctga
     1201 tcgggaaaaa catgcacatc accgggtcat gataattttc tgcaattgct gttataatct
     1261 gttgagtacg gtatgacatt gatttgaata tattagtgtt tatcgtatat ttacagatgt
     1321 ttaaatcatt gacacatggg aaatttcgga atcacggaaa taattcttgt aatcctggtt
     1381 attgtcctcc ttttcggcgg gcgtaagatt cctgagctta tgaaagggat aggtcagggt
     1441 atgaaggaat ttaaaaaagc atcgaaagtt gaggatgatc ctgaaaaaac caaaaccggg
     1501 tcttcagaaa ataagggaga ataaatttaa cttattgtgg ttcattccgg ctgtaccagg
     1561 ttaccaatgc gcctgcagtg caatgatcct gagtcttgtg tatcctgatg tgtcagactg
     1621 cagacgttca ggacgtgata gtttgcagat gaacggggaa gaagtgaacc tgaacctgcc
     1681 cgggtgatta tatctatgta ccacagaatg aaatcttgaa ttcaataaat tcctatgatt
     1741 accagaagga aatttttcag aaattctgct ttggcttcag caggtcttat tgccggtgca
     1801 ggttcagccg gcatggcatg cagcaacagg gataatcctg gcagttatga catcatggaa
     1861 gaggccttga agttccggaa gatcaatgca catgaacatg tttacctggg ctctgaacct
     1921 tcttctctca taaatgccgc cgatcgtctt ggaatggaaa agctggtggt atcacttccg
     1981 gttacagttg aagacagggt acttgtaaca ccagaaaaat tcagggaatg taataacctg
     2041 gtattaaaag caatgaagct ctttcccgac aggataatgg gccagtttta catcaatccc
     2101 tattattcaa aggaatccct ggaagaaatt gacaggtgtg tttctgccgg catggtctca
     2161 atgaagggct attaccaggt gaagataaat gatccgttat actatcccgt cattgaaaag
     2221 atgattgacc tgaaaatgat catccttatg catactcatt gcggaattgg ggtaggcggg
     2281 tacaggacca aatatggcaa tatacaggcc aatgcttcaa ctcccgatga ttttgccggg
     2341 gcagcagaaa gatatcccga agctttgttc cagtatgcac ataccggggg aggaggcgac
     2401 tgggaatatg cctgtaagac tttgagggat tatcctaatg tttatgttga tacatccggc
     2461 agcaataatg aaagcggaat gatcgaatat gcaatgaagc atcttggcga ggacaggctt
     2521 tttttcggga ccgacggttc atattaccag ggtgtgggtt cgattctggc agcaaacata
     2581 aatgatgacc agaggaaaaa gatttttttt gacaattata atctgattct gaaaagatcc
     2641 gggaaccatg ttgattgatg taagtgcgaa tatcggacac tggcccttca ggcagacagg
     2701 actgaaaaca tgcagggaat tgcttgggaa catggaccgg tacggagttg aaatatccgt
     2761 tatttcaaat atgaacggga tcttttataa ggattcacat gctgccaatg aagaactgta
     2821 ccgggaaatt tcatcggaaa agcggttcag gggacgcttt gttccctttg ctgttatcaa
     2881 tccgctatat gcagcatgga gacatgatct tcttgtctgc agcagggaat tgaaattcaa
     2941 agggctacgg ttatatccgc agtatcacga ttatgaaata acggatcctg cctgtatcga
     3001 catggtcagg tgtgcgaggg atgagggaat ggtcatcgct cttaccgtcc gcatggtcga
     3061 cagcaggctc cgttcgtgga tggatctcag ttcagaatgg atattgaagg atgtgattcc
     3121 actcattcgc gaagtgcccg acgccagata catgattttg aatgtatcgg gaggtataat
     3181 gttaaatgat gaggatacgg ctttgataag aggaacagat atgatcttcg atacttcggg
     3241 aaggaatata tctgacctgg gaagtttgct gaagaaatac ggcaatgata agtttgcatt
     3301 cggcacccac tccccgatac tcgattatct caccggattg ctcaggatcg aagcccttgc
     3361 ggacaatgaa gctgatgaat caaccaggga aatgctgaga tcgggaaacg caaagagact
     3421 tctcggaatc tgaaaatccg tttgagtgga ttcaggccgg gattttaatc agcacctcat
     3481 cttcatccat ggttattaat tcagaatggt ttttcaggtg gctgaccatg aaaggttaaa
     3541 catacatcag atgcaacctg ttgattgacc agaaacacga acatgaatga aaacactcgc
     3601 gttcagtcat gcgtggttcc ctgtccggga ggtcagacag gcatccgggc ttttaaaaaa
     3661 acagatacag atgaaaaaaa atcaaattgt tgcagcagct tcatttattg tgattgcttt
     3721 cctttccagc cctttacggc tgacagcaca ggaggaggat ctcagtgtgc tcagccgctg
     3781 gacagaatgg tcggatgcag gaaacatgct catacaccat cttaatgatc atgctttcag
     3841 atatcttgac agccgcgacc ggcatgttga atcactccgc acaagggaag actggaccaa
     3901 ccggcaaaat gaagtaagaa ataagcttat ggaggcagtc ggaccatttc ctccgaaaac
     3961 acctctgaat gcccggatca ccggagtgct taaaaaggac ggttacagaa ttgaaaagct
     4021 tatctatcaa tcaatgccgg ggttttacgt aacaggatgc cttttcattc ctgacggtat
     4081 caggggtaaa cgaccggcaa tcctgaatgt gataggacat actgatattt ctttcagggg
     4141 ggcggactat cagactctta tccttcatct tgttcagaaa ggattcattg tatttgccat
     4201 tgatccgatc ggacaggggg aacgacttca gtatttcgac ggtgagaaga atgttcccgt
     4261 tatcggtgca ggaacggctg aacattcgta tttcgggaat caatgtttca tggcgggtgc
     4321 ttcacctgca aggtatttca tttgggatgg gatcaggggt atagattacc ttgtatcgag
     4381 aaaggaagtg gatcccggcc gcataggcgt tacgggtctt tcaggcggag gaaccctgac
     4441 tgcatatatt tcagcgtttg acgacagggt aaaggcttcc gcgccatcat gctacataac
     4501 cggattcaga aggctccttg aatcaatagg tgcacaggat gctgaacaga atttctttca
     4561 tggcattaag tccgggatca ctcatgctga tcttattgag gtcagggctc caaagcctac
     4621 ccttattgta tcaacaacaa gagatttttt cagtatccag ggcgcacgtg aatccttcgg
     4681 tgaagcaaag aaggctttca tggcttttgg aatgagcgaa aatctcctta tggcggaaga
     4741 tcatttcggc catggattta caccgcgaaa taatgaagct acatgcgctt tttttcagaa
     4801 atacctcgac atgcccggaa atcccgcccc gggggtaatc agaaccctga acccggatga
     4861 actgaagata acggctaccg gacaggtctc aacttcattt gatgctgaaa cggttttcag
     4921 ccttaacgca aaggaagcag aaatgctgct tgcaaatctt gagaattcga gaaaagatag
     4981 tgaaaagcac ctggcacagg ttgaaaaaaa tgcagaactg cttagcggtt acaaccggta
     5041 tactgaaaaa cccgcgtcgg ttttcagggg aacatggcaa agggatggat attcggtgga
     5101 aatgtatgcg gtagggggaa acggcaagta tgtaattcca ctgcttctct tcattccgga
     5161 tggaaacggg aaataccccg cactgatcta tctgactccc aagggtaaag ccgcagatgc
     5221 aggagaggga gggcggatag aacaaattac caggaaaggt atcattgttg ctgttcccga
     5281 ccttcttggt atcggagaag ttgcacctga tgaatctttt tttaatgctt cttattttat
     5341 ttcggtacta actggcaaaa cccttatcgg gactggcgca gaggatatcg atgctattgt
     5401 taatttttta acagaacggg ccgatgttga tcatggcaga atttcctcag ttgcatttga
     5461 tgaactgtca ccggttctta tgcatgccgc cgttttcaat cactcgatag gttcaattgt
     5521 gctggcaggc tcccaggcct cattcaggtc aatggtcatg aacaggtttt acgaccggaa
     5581 gctggcacca tatactgttc cgggagcaat aaagtattat gatcttcctg atctgatcgg
     5641 gtgcattgca cccaggaaag tcgtgatggc aggtatcagg aaccagatga gagaatatgc
     5701 aggaaaggaa ttaattgaga ctgatctgac atttcccgtg aaagtctata ccctgaaagg
     5761 aaagccggat aatttcagga ttcttccgcc ggatgaagat atcctcccaa tgattgactg
     5821 gtgccttgcg aaataatttc acttttctga agtacatgga ggcgggtgac cacccttagc
     5881 tgccgtttct tctgacagga ataataattc agtttatcag tcggttgcga gaaagcgggg
     5941 agcgcttctg tgttttgttc cctgttcata gctgtatgct atttcaagca aaaccggttc
     6001 gctccaggcc cttccgaaga atgttatacc caccggtagt tcgtctataa atcccatcgg
     6061 caccgttatg gccgggtacc ccgaaattgc tgccggagaa gaactgccac cgccggaatg
     6121 atcgcccagg ataaggtccg ttttccaggc gggtgagcct gtcggcgcga caagagcatc
     6181 aagcccggct gaattcatta ctctgtcaat tccttcatca cgcgcggcac gcagcatctt
     6241 tctgagtgat tccctgtatt ctgctgaact gaggtcacct tttttatcag ccatctcaag
     6301 tattttctgg ttaaagaatc tgagttccac agggtctttc ttgttgaatt caatgagttc
     6361 cctcagggat tttacgcgag atccctctcc cagaccgctg agatatttgt tgaggccgtc
     6421 cttgaattcg tacagaagca cctgaaatga ggcatcttcg tacccggcgc catcaggtag
     6481 ttctacttca atgatttctg ctccccgggt tttcatgaag gcaattgctc ttttcatcag
     6541 ggtatcgact ttttcgtgga aacccgaacc ccattttatg atcccaagcc gttttccctg
     6601 cagaccgtct tccgaaagaa actgtctgta atcggtatgt gcgtgacctt ccgacaatag
     6661 tgttttcgaa tccgatggat cggtgccagt aagaatgccg aggcagatgg cggcatcttc
     6721 caccgttctg gccattggcc cgggagtatc ctgtgtgaat gagataggta ttatgccagt
     6781 ccggctgata agaccgacag tcggtttgat accgacgata ccgttattgt ttgacgggca
     6841 tactattgat ccgttcgttt ccgttccgat tgataccata cataaatttg ccgatactgc
     6901 agcacctgat cctgaactgg atccgcaggg attccggtca agcacatagg gattttttgt
     6961 ctgcccgccg acaccgctcc agccgctgga ggaaggcgag gcacggaaat tggcccattc
     7021 actcaggttc gccttaccta tgataacagc tcctgcttcc cttagctttc ttgccacccg
     7081 gctgtcggct ggtgcaatgg aaccggcaag ggctgtggcc ccggctgttg tcggcatctt
     7141 gtcgtgtgta tcaatattgt ctttcaggag cacagggact ccatgcatgg gccccctgat
     7201 ctttccctgt ttcatctctg cgtcaagttc cccggcaatc tgaattgcgt caggatttat
     7261 tgcaattacc gagttgagcc cgggtccctt ccggtcaatt tcatctattc tgccaagata
     7321 ggcggaaacc acatctctta cagtatatct gccttcctta tatccctgtt gcagcatgat
     7381 tatggtcatt tcctcaagcc atcttccttc cggatcaata tttactgtag gaggtttctt
     7441 cccctgatca catcccggca gcattatcag tattgtcagg ataattgtat aatgcagtat
     7501 gcctgtgtgt tgtattttca tcgttgtcag attataagta ttattaatat gtaagtcggc
     7561 gggaatgaca atttataaac agatattgaa acaagattaa aatttaaagc aatttaactt
     7621 aagaaaacat taatatttat gttttcaaag aaaataatac ttagatttga atatcctgaa
     7681 ctgatcatat tgtgcatggc tgataaatcc attgatgtga aagtgatcat gcaggctgga
     7741 ttatcatttc tgaaaaagga tgaacagcat ttacatcaaa ataccggaaa acgccgggga
     7801 tatttcagaa tatgtcctgg cacaggtgat tcgtaccagc ggttcaactc cacgtaagtc
     7861 cggaagctca gctttgttca acaggtatgg tttggttgcc ggaaccatcg ggggaggtgt
     7921 tcttgaggga aagatacagg ctgttgcgat ggatccctcg ataacaaaca agaccgggat
     7981 taatgaattt atcctcagca atactgccgg gggtgaggat gcactctgcg gaggaaaggc
     8041 cagtatattg attgattcgg aactgcagga gcattcggat gtttttggtg cccttaaaga
     8101 ttcatacaga gccaggattc cgggagtgct tataacgagg attctgaaaa ataaaaagca
     8161 ggaaacggag atatcccgtt actggctgac agaaaaaaca ggctctgaag taattggaca
     8221 ttttgccgat tcggtgcgaa atactgccat ggaaatgctt tcattactga aacctgatga
     8281 tttcagggaa gtcagtttgc aggcatcgga agaaaacctg tcggacactg tttatcttga
     8341 atccgtaata cctccgtaca ggctgcttat agcaggagcc gggcatattg ggaaagccct
     8401 tgccaggatt ggtcaaatga tcggatttga agttaccgtt gcagatgaca ggcctgagta
     8461 tgcgaattct gacaacattc cctgggccga tcatattgtt accgggaata ttggccgggt
     8521 ggtggccgac acggctaagg gaaaggatct gtatatcgtt attgttacca ggggacacag
     8581 ggatgatgcc gaggctctgg aggcatgtat tggatcggat gccgcctata tcggaatgat
     8641 cggaagcagg aacaaggtgg aattgatgcg cagggatttc atcgaaagga agcttgcgac
     8701 ggatgaacag tggaaacgga tatatgcccc ggtcgggctg gatattaatt cggaaaccgt
     8761 agaggagatc gcagtaagca tagccgccca gatcattcag atcaggaaca gctcccaaac
     8821 cctgaaaccc tgaaaccctg aaactcagag actgtggtac tttcaaattc agaaatatgg
     8881 gccattatac tggcagccgg agaatcgaaa aggatgaagg aacccaaaat gcttcttccg
     8941 gttcagggga aaacaatgat tgagaaaaca attaccaatg tcataacttc ggaagttaat
     9001 aatacccttg tcgtactggg tgctttcagt aatgagatat atgatgccat atctcattta
     9061 cctgtatccg tttgcattaa tgacaagtat gtggaaggga tgctttcatc agtgcagtgc
     9121 gggctatgca atattcccgg atcggccggg gcagtattgt tcgtccctgg cgatcagccg
     9181 ctgattgagc ccctggtgat taacagggtt attaatgcat accatgagtc aggcaaaggt
     9241 atagccatgc ctgtatacga aggtaaaagg ggtcatcctt tgctggtcag cgggaagtat
     9301 atggaagaaa taaagggttt cggcacgaat gaatcactcg gtcgcttgcg tgccggcatg
     9361 aggatgatgt gctggaggta attgttgaca ctccttcggt tttgaaagat tttgacacaa
     9421 gggaagatta cctggctgaa ataaataaat caaataaaat atagctaaca tggaagttaa
     9481 aatcaatttt accctcaacg gtaagaaaac aacgatcctt actgacccaa accagacttt
     9541 gctctgggtt ctgagaaacc agctgggact gacgggaacc aagtacggat gcgggaacgg
     9601 attctgcgga gcatgtaccg ttctgataaa caaggaaccg gtcagatcgt gcattacgcc
     9661 tgtcaccgat gtgtcgggga aagaggttat tacaattgaa ggactggccc gaaacgggaa
     9721 actgaatcct gttcagaagg cctttgtaga acatgatgca ttgcaatgcg gcttctgcac
     9781 gcccggaatg ataatgacag ccacgggaat gctgatgaaa aacccggcaa taacccggaa
     9841 ggaaataatt gaaggacttg aggaaaatct gtgccgttgc ggtgcacacg gaagaataat
     9901 tgatgcagtg gaatcagccg ccaaagaaat gaaaggagga aagtaatcat gaaaaataaa
     9961 gaaactaaaa ctgcagtaag acaggaagag tccacgaaaa acggtctcaa aagaaggaat
    10021 tttgttaagc ttcttggcgg gggtattttc cttttcttcc ggccctggaa aggattcgga
    10081 ggctcaggcg tatcgctctc ccagcagcga agaagactta ccactgacta taatgcattc
    10141 ctgcatatta ctcccacggg cattatcaat tgttatacgg gcaagattga gatggggcag
    10201 ggggttataa cggccctggt acagatgatg gccgacgaac tgaa
//
LOCUS       DAOOYG010000094         9735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_7605_length_9735_cov_6.971591, whole genome shotgun sequence.
ACCESSION   DAOOYG010000094 DAOOYG010000000
VERSION     DAOOYG010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9735
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7605_length_9735_cov_6.971591"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK288_08450"
     CDS             <1..240
                     /locus_tag="PK288_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPM18995.1"
                     /translation="LGYIQRGGSPSPMDRVLATRYGSSAADLIARREYGNMVALKNNE
                     IIPVPLAEVAGMLKLVENKDPLVVKARNLGTCFGD"
     gene            292..789
                     /locus_tag="PK288_08455"
     CDS             292..789
                     /locus_tag="PK288_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPM18996.1"
                     /translation="MKKLIFVRHGRAEEAGSGCPDIERSLTARGKKVSEQMALILLGK
                     KEDPGLFITSPAFRAYETCLIFARVFGHDPDKIVMKSSLYYKSGLKAFATVFEGIGNK
                     IDSIILFGHNPSFTEIPDRLSATGCDFVPKSGIVCLSFKSDTWEGIFHGKGKMEYFLK
                     PEKIS"
     gene            786..2852
                     /gene="ppk1"
                     /locus_tag="PK288_08460"
     CDS             786..2852
                     /gene="ppk1"
                     /locus_tag="PK288_08460"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPM18997.1"
                     /translation="MSRTYTPKEISWLSFNERVLQEAGNREVPLLERFKFLGIYSNNL
                     DEYFRVRVAGLRRLARLGSKSQTILGYNPKSTLKKVQEIVLEQNEKFEKIFAGLLQEL
                     ANHNIHIINEKELNKEQAEFVRSYFISEVRTRLMPFMIEKDAGLPNLRDDGIYLAIQL
                     YRNDTGKNRYALMEIPTQVLPRFVILPESNNGKYLIFLDDIIRSGLREIFFIFDFSDI
                     QAYTIKLTKDAELELTDDISESYIEKLSRSLQQRRHGSPVRFIYDRNMPAEMLKMLTR
                     VLKFGPDDVIIPGNRYHNFKDFMNFPNPGKKKFYFEPLVPIPHRDILPGKSILSVMKK
                     KDVMLFFPYHPFDHFIDLLREASIDPFVTSVHITLYRLARNSSVINALLNAVRNGKKV
                     TTVVELQARFDEEANILWGNKLMEEGVRVIYGVPGLKVHAKLCLITRVKGDITQRYAA
                     VGTGNFNEDTARLYTDHLLLTSNKKITNEVYKVFSFFNVNYRKDNFYHLVLSPFFLRN
                     RMTLLIENEIKNAKAGKKAYIWLKLNNLTDTDIINQLYEASNAGVTVRLIVRGMFSLV
                     PGVKNLSENIKAIGIVDRFLEHTRFMIFCNGGEEECFISSADLMPRNIDHRIEVTCPV
                     FDKNIRNELRHIFEIQWNDNVKARKLDPSLSNKFVKTGKEAVRSQTEVYRYLRKAASS
                     GSAKEK"
     gene            complement(2888..3028)
                     /locus_tag="PK288_08465"
     CDS             complement(2888..3028)
                     /locus_tag="PK288_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18998.1"
                     /translation="MKPFYQIESEETGTVILRRRRIAKALRWWLRENGCAFQHLFFLA
                     DK"
     gene            complement(3076..3693)
                     /locus_tag="PK288_08470"
     CDS             complement(3076..3693)
                     /locus_tag="PK288_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM18999.1"
                     /translation="MKRNSNLRKEESGFNISDLIRTKEEKMFDQLEPVSREVSDMASE
                     GGETFISYIRSIGLAGENNLMVLPASHHYYYEKSEMTDVNTLVNLRTLNMMGNLESFL
                     KTLMSVLPPNTNFIGCFSDSGSSGLRNGNKARKSSVFYQRLINLLDSRKVHYMTRNDV
                     FSLLEKHGFSVVDMTDIGGLTYFYSQNAPGEAEFRASIETPVKYN"
     gene            4228..4752
                     /locus_tag="PK288_08475"
     CDS             4228..4752
                     /locus_tag="PK288_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPM19000.1"
                     /translation="MVFTAMMIGTAGISQTSGFYDFKVKTLEGADFDFASLKGKKVMI
                     VNTASKCGFTPQYKDLEELYEQYGDKLVIIGFPANNFANQEPGSASEIRQFCDSKYGV
                     TFPMMEKISVKGRDMHPVYQWLTSKEKNGVMDSEVRWNFQKYLIDENGRLVDVHYSKE
                     KPGSERTLAWLGVR"
     gene            5053..7254
                     /locus_tag="PK288_08480"
     CDS             5053..7254
                     /locus_tag="PK288_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPM19001.1"
                     /translation="MTRLFTALIGFCITLAALQAQTGNLGLHGKVTWLGDNRIRVEYD
                     WSDDSQLLDWTPTDGSVLIRGNGTLTISGGTVPVHSMILKQPMKCTRINAQNAKSINA
                     ETAHLNFITNVAGWNGVNFNPPEIIGVIYNADANYWLENESYSSFGARYITSGTSYNV
                     EITIEGSTITAKSSLSGNTYTHSLDSPADPDREVAVGGWGGDTEWGTLTIEGEVNTTW
                     QPRADMIDIQSCGASFAPVIEVTGNPQIEWIFYDGTTSSAAGPSKNYGSPGIRHNLLK
                     VTPWSALIGINAGYDGADGGYGGFARIAAQHVRGFSNLSLAGEGLQYICANYSPLAEL
                     DLRGLNNLRFVEMYLCQNLNTVRLGNHPFLERICTENCNLTALDISGCPSLKDLRAAN
                     NNYRAINWGGTGSMLEHICVRSNPQITENIPELSRFTMLRELLIWDAGQTGSLVCHSP
                     VIRTIEAYNNHYSDADVSGCTVLEQLQLSGSRLASIDVSGVKGLILVELKDCGLAQPM
                     VDHVLKTLDEAGWFEGTLDLSGNSAPSAAGMTHYNNLTGREWTVSITDPGQKIPVQEI
                     TVTGENGSSAISTDNGALQLTASVWPVYASDKSLTWSVVNGARFAEVDANGRVKALKN
                     GSAKVRATANDGSGIYGEITITITNQSAGDIINYNIGSIIVSATELQILFEQDFTSWK
                     ASIYNLSGNIVVSRMVDGSRMTFDISRLTPGLYIIAITRGNSVRVAEFVKP"
     gene            complement(8759..8833)
                     /locus_tag="PK288_08485"
     tRNA            complement(8759..8833)
                     /locus_tag="PK288_08485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8796..8798),aa:Pro,seq:tgg)
     gene            complement(8810..9085)
                     /locus_tag="PK288_08490"
     CDS             complement(8810..9085)
                     /locus_tag="PK288_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPM19002.1"
                     /translation="MSFYVYILESVITGKYYVGQTSNLEERLQRHNSGRNQSTRSGVP
                     WELKWWKEFESRSASMEEESRIKKLKKRKGIERHVLKNGYRGVAQPG"
BASE COUNT         2673 a         2262 c         2306 g         2494 t
ORIGIN      
        1 ctgggctata tccagagagg agggtcgccg tcgcccatgg acagggtcct ggctacaaga
       61 tacggatcat cggcggccga cctgattgcc aggcgggaat atggaaacat ggtggccctg
      121 aaaaacaatg aaataattcc cgtccctctt gccgaagttg ccggcatgct taaactggtg
      181 gaaaacaaag acccgctggt ggtaaaggct aggaacctgg gtacatgctt cggtgactga
      241 tacgcgaatt attacaattc tgaagtccgg aacctctgat acttataaaa catgaaaaaa
      301 ctgattttcg tccgtcacgg aagggccgag gaagccggat ccggctgtcc cgacattgaa
      361 aggtcgctta cagcaagagg gaaaaaggtt tcggaacaga tggccctaat cctgctcggg
      421 aaaaaggagg atccggggct gtttatcaca agcccggcat tcagggctta cgagacatgc
      481 ttaatatttg ccagggtgtt cggccatgat cccgataaga tagtaatgaa aagcagcctt
      541 tattacaaaa gcggactgaa agccttcgca actgtcttcg aagggatcgg caacaagatc
      601 gacagcatca tccttttcgg ccataatccc tcatttacgg aaatccccga caggctgtcg
      661 gcaaccggct gcgatttcgt gccgaaaagc gggatagtct gcctttcatt taaaagcgat
      721 acatgggaag gcatattcca tggaaagggg aaaatggaat acttcctgaa acccgaaaaa
      781 atatcatgag caggacatac acaccaaagg agataagctg gctttctttc aacgagcgcg
      841 tgctgcagga agccgggaac agggaggtgc ccctgctgga acggtttaaa ttcctgggca
      901 tatattccaa caatctcgat gaatatttca gggtaagggt ggccggacta agacgccttg
      961 cgaggctggg cagcaaatcc caaaccatac tgggatataa ccctaaatcg actctgaaaa
     1021 aggtccagga aatagtgctt gaacagaatg agaagtttga aaaaatattt gcaggtcttc
     1081 tgcaggaact ggcaaatcac aatatccata taatcaatga aaaggagctg aacaaggaac
     1141 aggctgaatt tgtcaggtcg tattttatca gtgaggtgcg tacaaggctc atgcccttca
     1201 tgatcgaaaa ggacgcaggg ctcccgaacc tcagggatga cgggatctac cttgcaatac
     1261 aactttacag gaatgatacg gggaaaaaca ggtatgcact tatggagata ccaacgcagg
     1321 tgctgccaag gtttgtcatt ctgcccgaga gcaataacgg gaaatacctg atcttccttg
     1381 acgatattat caggtccgga ctgagggaaa tattcttcat tttcgacttc agcgacatac
     1441 aggcctatac catcaagctg accaaggatg cggaacttga gttaactgat gatatatctg
     1501 agagttacat agaaaaactg tcgcgcagtc ttcagcagcg aagacatggc agcccggtaa
     1561 gattcattta tgaccgcaac atgcctgccg agatgctgaa aatgctgacc cgtgtcctca
     1621 aattcggacc cgacgacgta attataccgg gcaacaggta tcacaacttc aaggatttca
     1681 tgaattttcc caatccggga aaaaagaagt tctattttga gcccctggtt cccatacctc
     1741 accgcgatat cctgcctggt aagagcatcc tgtcggtaat gaagaagaaa gacgtgatgc
     1801 tgttctttcc ctatcatccc tttgatcatt ttatcgacct gctcagggaa gcttccatcg
     1861 atccgtttgt aacatccgta catataaccc tgtacaggct ggcaaggaac tcgagcgtga
     1921 taaacgccct gcttaacgct gtgaggaacg gcaaaaaggt aaccaccgtg gtggaactcc
     1981 aggccaggtt cgacgaagaa gccaatatcc tgtggggaaa caagcttatg gaggaaggcg
     2041 tcagggttat ctatggtgtg cccggactga aggtgcatgc caagctgtgc cttataacga
     2101 gggtaaaggg cgacatcacc cagcgctatg ccgcagtggg aacaggcaat ttcaacgagg
     2161 atacggcccg gctttatacc gatcacctgc tgctgaccag caacaaaaaa atcaccaatg
     2221 aggtatacaa ggtattcagc ttcttcaacg tcaattacag gaaagacaat ttctatcacc
     2281 tggtactgtc accgttcttt ctcaggaaca ggatgaccct tctgatagaa aacgagataa
     2341 aaaatgcaaa ggcgggaaag aaggcatata tttggctgaa attaaataat ctgaccgaca
     2401 ctgacattat caatcagctg tatgaggcca gcaatgcagg tgtaactgtc cgccttattg
     2461 tccgtgggat gttttccctt gtgccgggtg tgaaaaacct cagtgagaat ataaaggcaa
     2521 ttggaattgt ggaccgtttc cttgaacata cccgtttcat gattttctgt aacgggggcg
     2581 aggaggaatg cttcataagc tccgccgacc tgatgccaag gaatatcgac caccgtatag
     2641 aagtcacctg tcctgttttc gataaaaaca tccgcaatga actcaggcat atttttgaga
     2701 tccagtggaa cgacaatgtc aaggcccgga agctcgaccc gtcgctttcg aataagtttg
     2761 taaaaacagg aaaggaagca gtccgttcgc agacggaagt ttacaggtac ctgcgcaaag
     2821 cagccagttc cggttctgcg aaggaaaaat aatcagatga gcccgctgcg gcggtacacg
     2881 atccgcatta tttatccgcc aggaaaaaga gatgctggaa agcacatccg ttttcacgca
     2941 gccaccagcg taatgccttg gctattcttc ttcttctcag aataactgtc cctgtttcct
     3001 ctgattctat ctgataaaac ggtttcatag atacgatgaa ttaaatgtga ttatttttca
     3061 ggtattcact cggaattaat tgtatttcac aggtgtctcg attgaggccc tgaactcggc
     3121 ttcgcccggt gcattctgtg aataaaagta tgtcaggcca ccgatgtccg tcatgtcaac
     3181 aacactgaat ccgtgttttt cgaggagact gaatacatcg ttccttgtca tgtaatgaac
     3241 ttttctggaa tcgagcaggt tgataagccg ctggtagaaa accgacgact ttctggcctt
     3301 gtttccgttc cttaaacctg aacttccgct gtcggaaaag caacctatga aatttgtgtt
     3361 cggcggaaga acggacatca gggttttgag gaacgattca agattaccca tcatattgag
     3421 agtacggagg tttaccaggg tgtttacatc ggtcatctcg cttttctcat aataataatg
     3481 atgggacgcc ggcagaacca tgaggttgtt ctcgcctgcc agcccgatgc tccttatata
     3541 acttatgaag gtctcgccgc cttcagatgc catgtctgat acttccctgc ttacaggttc
     3601 aagctgatca aacatttttt cttccttggt ccttatcagg tctgaaatat tgaagcctga
     3661 ttcttctttc ctgaggtttg aatttctttt catctctttg cgttttaatt gtcatagtaa
     3721 aaataggtcc ggaaataatg cctgacaata cgcaaaagcc catctagtgg ggattagaga
     3781 tgattttaaa aactctttgt caaaggaatt ccggccgtcg ttgaactgta ttcacaactg
     3841 acgggaaaaa catggctgtt cgtcaaaagg aggccgttat ttaccataat ccggagccga
     3901 aatgaggttt tatctttatt gttaattata taacacatat attcaaatag ttacgagcat
     3961 gatttatcac aattgcctga aatacccggt tttgacaaac gccctgaatg tttagactaa
     4021 aaaatggatt ttcggccttt tctgcagcat ttttccgggg ccgcgaagcc ggaaaacgtc
     4081 agaaatttat ctgcgcggcc tgtaatttac ttccgggatg agtgcagcca taatgaattt
     4141 catcgtaatt ccgtgaactt tcatccggat tgcatgttta ctgatcggaa ctgaaatttt
     4201 aaacatacta taatgaataa actgattatg gtctttacgg caatgatgat aggaaccgca
     4261 ggtatatcgc agacctccgg cttttacgat ttcaaggtaa agacccttga aggtgcagat
     4321 ttcgattttg cctccctgaa aggcaaaaag gtgatgatcg tgaacactgc ctcgaagtgt
     4381 gggttcacac cgcagtacaa ggaccttgag gagctttatg agcaatatgg cgacaagctt
     4441 gtgattatcg ggtttcccgc aaacaatttt gccaaccagg aaccgggcag cgcttccgag
     4501 atcaggcaat tctgcgattc aaagtacggt gtcacctttc caatgatgga aaagatctcc
     4561 gtgaagggca gggatatgca tcctgtgtac cagtggctca catcaaaaga gaaaaacggt
     4621 gtaatggatt cggaggtcag gtggaacttc cagaaatatc ttatcgatga aaacggcagg
     4681 ttggtggatg ttcactattc caaggagaaa cccggatccg aaaggaccct tgcctggctg
     4741 ggtgtcagat aacccggtaa tgtgatctca gccgggaaaa cccggaaaac cgccggaagc
     4801 gctcgttaat ccgtgtgtat ccctgtcagg ctgaagcttt cccgcatttc catgacccgc
     4861 cattaaccgg aggcatccgg tacaggtctt ctgcattcaa caaattcaaa tacccgctga
     4921 tttacgtgac gcatctgtca gctcattggt tgttcaccgg tatgctgtca cataacctgc
     4981 catagtctga aaaaccattc attgccgggt ttatccaaat attcctattt ttgtcttttt
     5041 gtttttgtat tcatgacaag acttttcacg gcattgattg gattttgcat tactcttgcc
     5101 gcattacagg cacagacagg taatctgggg ctgcatggga aggttacctg gctcggcgac
     5161 aacaggatac gcgtggaata tgactggtca gacgattccc agcttctcga ctggacccct
     5221 accgacgggt cggtccttat ccgcggaaac ggaacattga ccatcagcgg aggtacggtt
     5281 ccggtacatt caatgatcct gaaacagccg atgaaatgca ccaggatcaa tgctcaaaac
     5341 gcaaagtcaa taaatgccga aactgcgcat ctcaatttca ttacaaatgt agcgggctgg
     5401 aacggagtta acttcaatcc gccggagatc atcggggtca tctataatgc ggatgcaaac
     5461 tactggcttg agaatgaatc atattcaagt tttggagccc gttacatcac atccggcacc
     5521 agttacaacg tggaaattac cattgagggt tcaacaatta ctgcaaaatc ttctcttagc
     5581 ggcaatacat atacacattc gcttgactcg ccggctgatc ctgaccgtga ggtggctgtt
     5641 ggcggctggg gcggagatac tgaatggggt accctgacaa tagagggaga agtcaacaca
     5701 acatggcagc caagagccga catgatcgat attcagagct gcggtgcatc ttttgctccg
     5761 gtaattgagg ttacaggcaa tccgcagatc gaatggatct tttatgacgg aacaacttct
     5821 tctgcagccg gtccgtcaaa gaattacggc agcccgggta tacgacacaa cctgctgaag
     5881 gtcacaccgt ggtcggcgct gatcggtatt aatgccgggt atgatggtgc tgacgggggc
     5941 tatggaggat ttgccaggat agccgcccag catgtcaggg gattcagcaa cctcagcctt
     6001 gcaggagaag gcctgcaata tatttgtgca aactacagtc ctcttgcgga actggatctg
     6061 agggggctta acaacctcag gttcgtcgag atgtatctct gtcagaatct caacactgtc
     6121 aggctcggaa atcacccttt cctggaaagg atctgtactg aaaactgcaa ccttacagcc
     6181 cttgacattt ccggatgccc atccctgaag gatctgaggg cggctaacaa taattacagg
     6241 gcaataaact ggggaggaac aggatctatg cttgagcata tctgcgtcag gtcaaacccg
     6301 caaattaccg aaaacattcc ggaactgagc cgtttcacca tgctccgcga actgcttatc
     6361 tgggatgccg gtcagacagg ctctcttgtc tgtcacagtc cggtcataag aaccatcgag
     6421 gcctataaca atcattactc cgatgcggac gtaagcggct gcacggtact ggaacaactt
     6481 cagctttccg gaagccggct tgcttcaata gatgtttccg gtgtaaaggg acttatcctt
     6541 gtagaattaa aggattgcgg actggcacag ccaatggttg atcatgtact gaaaaccctt
     6601 gatgaagcag gctggtttga aggaaccctc gatctttccg gcaattcagc tccctctgcc
     6661 gcaggcatga cccattataa taatcttacc ggcagggagt ggaccgtcag tatcaccgat
     6721 cccggtcaga aaattccggt tcaggagatc accgtgacgg gtgaaaacgg aagttcggca
     6781 atcagtaccg ataacggagc ccttcagctg acagcttctg tatggccggt ttatgcatcg
     6841 gacaaaagcc tgacatggag tgtagttaac ggcgccaggt ttgcggaagt tgatgcgaac
     6901 ggacgggtaa aagcccttaa aaacggctcg gctaaagtaa gggcaacggc aaacgacgga
     6961 tcagggatct acggtgagat aactatcaca ataaccaatc agagtgccgg ggatatcatt
     7021 aactacaata ttggcagtat cattgtatct gcaaccgaac ttcagatatt gtttgaacag
     7081 gacttcacat catggaaggc aagcatatac aatctcagcg gaaacattgt tgtcagccgc
     7141 atggttgacg gcagccggat gactttcgat atttcccgtc taactcccgg attatatatt
     7201 attgccataa ccaggggaaa tagcgtcagg gttgcggagt ttgtaaaacc atgacccgta
     7261 tgagagatga gagggcagaa ttattgtgag gtgccctgaa tatagtgttc cggtttaaaa
     7321 gatggtagtg ccgttaatcc tgctgatatg caagccgtac aaaccagggc cgcgcgggca
     7381 atccggaaaa ggcgcgaacc gctccctgtt ttttggagcg ggaaagggcc ccgcgcaaat
     7441 ggcgaaaaac aggagcggtt gtcttttccg ggctcctttt ctttggtgac tttcctcccg
     7501 gcttgcaccg ggggttatta actcaagttt cgcagttctt ttaaatgttg aaaatcatgt
     7561 aggaattatt taaaaaagta acatgatttg taagttaata tactgtttct gagtatatta
     7621 acaatgtctt caaaaacatt caaccaggct gcagagtaaa gatattattg caattccgga
     7681 aataaaaacg gtttttccat aggtggcatg aaccggattt taacttaatg cctgtttctt
     7741 ttcaggtttc tgcctgttca gggagatccc tcatttcgca gatccttcgc acactcaggc
     7801 tctgctcatt cgggatgaca ggcatgaggt gtaataaacg ggggaagaaa cggcgggcca
     7861 cggcagggtg attaattaca ttcagaattc cgttctgtgg cccgccgttt cttccccccc
     7921 tgaccacact gatcaccgta tcatcccgat cccggttcac cgggagaggg atcccccttt
     7981 tcaggtaccg gagatgataa atggcttttg agctgataat gttttatgga acacttcagg
     8041 tgttctgccg gaccagagat ctgataatgg cggcaaaaac gaatccgctc cgcaggaaag
     8101 tgaaccggaa atccggaagt tattggatag aactgccagg agattgaata tcaatatcat
     8161 aatgaagata cataagctta taaacgatta aatttttgtg tattgttata ggttgcgaaa
     8221 cttgagttat taatatatgc gctctttcag agcttgcttt cagtggtccc ggaaaccata
     8281 tgtcctatac aaaggctttg acatcggaaa attaccttct aaatcgaaat agcagaatgc
     8341 tgaatccggc ggcgcgcggg caatccggaa aaggcgcgaa ccgctccctg ttttttggag
     8401 cgggaaatgg ccccgcgcaa atggcgaaaa acaggagcgg ttgtcttttc cgggcttctt
     8461 ttctttggtg actttctttt ggagaagcaa aagaaagtca catttaaatg cccatagcaa
     8521 tcactgcatt tatcatgcaa gtccggcgca aaacaaaaaa aggccctgaa ttcgctgccg
     8581 aaaaatgaaa aggcaggatt atttcatgat cctgaccggg ggtgacttaa aagaaaaagc
     8641 cctgaattcg ctgcgctcat tacagtgttc tgtttttcaa cacttttgaa agcgagcttt
     8701 caacgtattg aaaaacagaa ccctgtcctc cttgcgtcgg acagggcttt ttcttttgtc
     8761 ggggtgacag gattcgaacc tgcgaccctc tggtcccaaa ccagatgcgc tagccgggct
     8821 gcgctacacc ccgataaccg ttttttaata catgcctctc aatacctttt ctttttttca
     8881 atttctttat cctgctttcc tcttccatcg acgcagaacg gctctcaaac tccttccacc
     8941 atttcagttc ccagggaaca ccgcttctgg tcgattgatt cctacctgaa ttatggcgct
     9001 gaagccgctc ttcaagattc gatgtctgcc ctacatagta cttgccggtg ataaccgact
     9061 ctaatatata aacataaaaa ctcattcaac atttgcgcta atcccgatcc ccgttccacg
     9121 gggtcgggga tcctggtcac acaacgtgtg acgggaccgg gctgcgctac accccgataa
     9181 ccgtttttta atacatgcct ctcaatacct tttctttttt tcaatttctt tatcctgctt
     9241 tcctcttcca tcgacgcaga acggctctca aactccttcc accatttcag ttcccaggga
     9301 acaccgcttc tggtcgattg attcctacct gaattatggc gctgaagccg ctcttcaaga
     9361 ttcgatgtct gccctacata gtacttgccg gtgataaccg actctaatat ataaacataa
     9421 aaactcattc aacatttgcg ctaatcccga tccccgttcc acggggtcgg ggatcctggt
     9481 cacacaacgt gtgacgggac cgggctgcgc tacaccccgc gtcgcccgct gtagccatgg
     9541 caaaggcgga cagctgaaag tgccgcaaaa atataacttg ttttttgatt atcaaatccg
     9601 ggcagtattt tttcggaggc tgatactgcc gttaaacacc atagcaggct gtctttactt
     9661 cctctaacca ctcattattc atgcgaatta agagttgact tagaataagt tattaacata
     9721 taaaatacag attgt
//
LOCUS       DAOOYG010000095         9674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_7677_length_9674_cov_6.303670, whole genome shotgun sequence.
ACCESSION   DAOOYG010000095 DAOOYG010000000
VERSION     DAOOYG010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7677_length_9674_cov_6.303670"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PK288_08495"
     CDS             complement(<1..276)
                     /locus_tag="PK288_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPM19003.1"
                     /translation="MFENLSEKLEKSFKLLKGQGRITEINIAETLKEVRRALLDADVN
                     FRIAKQFTDTVRAKALGQEVLKSVNPSQMMIKIVHDELVALMGGDKSD"
     gene            complement(303..2090)
                     /gene="argS"
                     /locus_tag="PK288_08500"
     CDS             complement(303..2090)
                     /gene="argS"
                     /locus_tag="PK288_08500"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPM19004.1"
                     /translation="MEIVLKQKIREALSSLYNAKVDDNHIQLQETRKDFPGDFTLVVF
                     PLLRFSKNTPEQTGESIGNYLTDNFPLISGFNVIKGFLNLEVSYKWWIEYFSALCGEK
                     DYFRNCRIANPEVILVEYSSPNTNKPLHLGHIRNNLLGFSLNRILASCGHKVIKTSIV
                     NDRGIHICKSMLAWLKFGNNETPESAGIKGDHLVGKYYVKYEQEYKLQVDELIKNGMG
                     EAEARSSAPLLKEAREMLLRWESNDRETMELWKMMNSWVYAGFDETYRMLGVDFDRVY
                     YESETYLAGKEIVLKGLRDNILYRQEDNSIWVDLTSMGLDHKLLLRSDGTAVYITQDL
                     GTAVERFNEFGFDKTIYVVGNEQNYHFQVLGALMKKMGYPWAGGLIHFSYGMVELPEG
                     RMKSREGIIVDADDLIREMHDTAREVSLELGKLSDYSEPEKEEIFRKIGLGALKYFML
                     KVDPHRNMTFNPAESIDFNGNTGPFIQYTYARIRSLFRKAEQMGIYPDKKALLTARPG
                     QKEIELIKLFRKFSDVVSQAASELSPALVANYCYDLSKEYNQFYHDFSILGEENMELR
                     DFRIYLSEVAGDLILNGMWLLGIEMPERM"
     gene            2306..2779
                     /locus_tag="PK288_08505"
     CDS             2306..2779
                     /locus_tag="PK288_08505"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaC family regulatory phosphoprotein"
                     /protein_id="HPM19005.1"
                     /translation="METTQPAMKELKHMFVEQTEKTPLVDFNNLTGELILSGRSIPIN
                     APRIFEPILEWVNNYIKDPRPTTNLRLNLEYFNTASSIWLAKIVKALTNIEKQDCVLF
                     LHVYFSLEDFDDIDDIKDDLSPVIDVVSNSKVSVGLKIYGTDEAGKIMKESMIFI"
     gene            2914..4377
                     /gene="amrB"
                     /locus_tag="PK288_08510"
     CDS             2914..4377
                     /gene="amrB"
                     /locus_tag="PK288_08510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPM19006.1"
                     /translation="METYSQPGSADRYPAVAGRFYSADKETLRNDISKLFENCKKPVD
                     HKHVRAIISPHAGYIYSGRVAASAFSAIGKKTVYKNIFIIGSSHIMAFEGASAYYTGD
                     FVTPLGKIRVNREIANSLRSNRLFNHPVSAHLQEHSLEVQIPFIQYYLTGDPMIVPVI
                     IGTNNINSIKKIAEALKPWFNSDNLFIISSDFSHYPGYKTACEVDKATADAIISGDPQ
                     NFLNTLKKNSLRNEPGLATSMCGWTSGLLLMYLAEQEGNLEFSKVDYCNSGDSKYGNK
                     DEVVGYNAIILAEKKIPGHSTGSLYDEISFTAREKEELIALARNNIRSLLNDRKMITV
                     DENKLPAIFKQPLGAFVTLKIDNVLRGCIGKFISSDPLFRVVLESSKSSAFEDPRFPP
                     LTKGEFAKTDIEITVLGPLKKITDINEIILGKHGIYIKKDFHSGTMLPQVAKENGWTV
                     EEFLGYTSRDKTGIGWDGWKKADIFVYEGIVLDEIKK"
     gene            4545..6809
                     /locus_tag="PK288_08515"
     CDS             4545..6809
                     /locus_tag="PK288_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19007.1"
                     /translation="MHTAPEWDAAFLKSMAGQHVVLGMAFISTGLLHFFLHYSRFRNL
                     FKKRESDAPNEFYCRTQRTRGAIGANLFAVGFVSSAIQLLLLREILNISGGYELIAGT
                     FLGSWLMASAAGSAIAPKTSITNILSINLLFFSGPVISVFLLIILSRLFLNPGETPSY
                     LASIIFTLIVLVPFCFISGFTFIKLVSEASSIKDFRPGRSFAVETSGGIAAGILISFA
                     SHGGINNYQAIILIIISGFSYTVISQSGLNRRNLMLTGIIVLALSAGVIISAPDKYFR
                     QLLLRGLTVTDSSDTPYGNITRGMYGEEKSTYYDHRLLFYSDDIIEREEDIHYPMLQL
                     DNPEAVLLVSGSLYPHLGEINKYKAKRVVYVERDPALIKAESSTEPFKIPDLLVENKD
                     AYTFIKNAGEKFDAVILLLPPPSSLLLNRFYTYEFFMHVKKVLKPEGVFSCAPGINPD
                     YLNIESVLFFSSIYNSLKAVFRYVVPVGGNKLYLLAADKEISISFCELTEKKGISNLY
                     VGPDYLADDLTELKTDQIISVIDESVRLNHILTPVATFYFQAYNISKSINEKLPLIIL
                     LALLFLLPLLTVRRTNMLMYFSSSALAGYEIILLLVLQSSVGNMYQVTGLILAGLLSG
                     LAVGSGLSIPFLESRTYRIKSWLLIICYLAMYFAIKRLVDSDSNVFVTVALIISGFIP
                     AAITGNLFREFTQTAMVPSVPSPVYSADLAGSAAGCIVFSGFAVPLIGIGPSLLIFPV
                     LVLTGYLFALKARK"
     gene            6877..7995
                     /gene="amrS"
                     /locus_tag="PK288_08520"
     CDS             6877..7995
                     /gene="amrS"
                     /locus_tag="PK288_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPM19008.1"
                     /translation="MISKRNFVKRCAALSTGLICVPYGSLLSEMSSVQAGIYKKEAMF
                     QEETPGGIMCMICPNGCVLREGEVSRCRSRKVRKSKLYTLAYGSPCSVNVDPMEKKPL
                     YHFLPGSRAFSIAAAGCNLSCLNCQNWTISQTSPEKTRNYDLMPDKVVEECISRGCKS
                     IAYTYSEPVTFYEYVYETAIVARKAGIKNIVKSNGYINPEPLKKLCSVFDAANIDLKS
                     FNESTYLKLTGGRLQPVLDSLKIYRDMGIWLEITNLVVPTWSDNLDEIRKMCRWLSDN
                     GFKKVPIHFSRFFPVYKLEQLPPTPIETLNRAASIASEEGLLYVYTGNVPGNELSDTK
                     CPSCKKPLISRQGFRVTSNTIRDGKCDSCGRIIDGVWN"
     gene            8036..>9674
                     /gene="ligA"
                     /locus_tag="PK288_08525"
     CDS             8036..>9674
                     /gene="ligA"
                     /locus_tag="PK288_08525"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPM19009.1"
                     /translation="MNQTEARKRIEKLRREIEEHNRNYYVFNDPVISDFEYDLLINEL
                     ETLEKQYPEFYSEESPTRHVGSDLTREFRQFEHKYPMLSLGNTYSIEEIRDFDSRVRK
                     SVMGEISYVCELKFDGASISISYSGGSMEKALTRGDGTRGDDVTENITTIKSIPGRIG
                     SDAVPASFIARGEILMPRSVFKELNEEREKEGLNLFANPRNAASGTLKILDPSLVAAR
                     KLDCLFYSLLGDDLPADNHYDNLKKLQEWGFPVSDSYRLCRNLDEIIGFIQFWETRRN
                     DLPFDTDGVVIKVNSLDQQLQLGFTAKSPRWAIAYKYKPERALTKLLFVSYQVGRTGT
                     VTPVANLEPVQLAGTTVKRASLHNADQISLLDLHEGDMVYVEKGGEIIPKIVGVDKAS
                     RSDTSMAISFIEECPECGTPLVRNEGEANHYCPNILHCPPQIKGRISHFISRRAMNIE
                     GLGEETTDLLYTRGLIRNAADLYDLKAGELASLEGLGEKSASNIIRSIEDSRNVPYYR
                     VLFALGIRHVGETVAKKISVRFRSIDKLISAGISEITE"
BASE COUNT         2753 a         2163 c         2069 g         2689 t
ORIGIN      
        1 atctgatttg tcgccaccca ttaatgctac cagctcatcg tggacaatct tgatcatcat
       61 ctgtgacgga ttcaccgatt ttaggacttc ctgacctaga gctttcgccc tgacagtatc
      121 ggtaaactgc ttcgctatcc tgaaattgac atcggcatca agaagagccc ttcttacttc
      181 tttgagggtc tcggcaatat ttatttcagt tattctgccc tgccccttga gtagtttaaa
      241 ggacttttcc agcttttcgc ttaaattctc aaacatatta ttatcaaggt ttattactta
      301 attcacattc tttcaggcat ctcaatcccc agaagccaca tcccgttcaa aatcagatca
      361 cctgcaacct ccgaaagata tatcctgaaa tccctcaatt ccatgttctc ctcacccaat
      421 attgaaaagt catgataaaa ctgattgtac tcctttgaaa ggtcatagca gtagtttgca
      481 acaagtgcgg ggcttagctc tgatgccgcc tgggaaacaa catctgaaaa cttcctgaac
      541 aatttgatca gttcgatctc tttttgtccc ggcctggcag taagcagtgc ctttttatcg
      601 ggataaatgc ccatctgttc agcttttctg aataatgatc ttattcttgc ataggtgtac
      661 tggatgaagg gtccggtatt tccattgaag tctatggatt cggcgggatt aaatgtcata
      721 ttcctgtggg ggtccacttt cagcatgaaa tatttaagtg ccccgagtcc tattttcctg
      781 aaaatttcct ccttttccgg ctcagaatag tcagacagct tgccaagttc cagagagacc
      841 tcacgggcag tatcatgcat ttccctgatc aggtcatccg catcaacaat gataccctcg
      901 cgtgatttca tcctgccttc cggaagctcc accatcccgt acgagaaatg aataaggccg
      961 ccggcccatg gatatcccat ctttttcatc agcgcaccga gcacctggaa atgataattc
     1021 tgctcattcc cgacaacata aatggtttta tcaaatccga attcattaaa tctttccacg
     1081 gcagtaccca gatcctgggt tatataaacc gccgttccgt ccgatctgag aaggagtttg
     1141 tgatccagcc ccattgacgt caggtcaacc catattgaat tatcctcctg ccggtaaagt
     1201 atgttatccc ttaatccctt cagaacaatt tcttttcctg cgagataagt ttccgattca
     1261 tagtaaaccc ggtcaaagtc aactccgagc atcctgtaag tctcatcaaa cccggcataa
     1321 acccatgaat tcatcatttt ccacaactcc atggtttccc tgtcgttcga ctcccatctc
     1381 agcagcatct ccctggcctc cttaagcagg ggtgcagaac ttcttgcttc agcctcgccc
     1441 ataccgtttt tgataagctc atccacctgc aatttgtatt cctgctcata tttcacataa
     1501 tatttgccaa cgagatggtc ccctttaatc ccggcagact ccggagtctc attgttacca
     1561 aatttaagcc aggccagcat tgacttgcaa atatgtatcc ccctgtcgtt cactatgctg
     1621 gttttgataa ccttgtgacc gcacgacgca aggatgcggt taagcgagaa tcccaggaga
     1681 ttattcctga tatgtcccag atgaaggggt ttgtttgtgt ttggagatga gtactcaaca
     1741 agaataactt cagggtttgc aatcctgcaa ttcctgaaat agtccttttc cccgcacaag
     1801 gccgaaaaat actcgatcca ccacttgtat gaaacttcaa ggttaaggaa tccttttatc
     1861 acattaaatc ccgaaataag agggaagttg tcagtcaggt aattgcctat gctttcgccg
     1921 gtctgctcag gtgtattttt tgaaaaccga agcaaaggaa aaaccaccag tgtgaagtcg
     1981 cccggaaaat ctttccgggt ctcctgcagc tgaatatgat tatcatcaac ctttgcattg
     2041 tataggctgc ttaacgcttc cctgatcttt tgcttcagaa caatctccat tgatgttaaa
     2101 atattcgcat gcaaatataa catttaaata tttccgccgt ataagcccat ggattcgcgt
     2161 agaatgagtg gctgttttgc aggcattact gattttttat accaaacaac agttttattt
     2221 gacaatcatt catttctttt tgataaaaag ttacatcact aatgaaaaga aattttactt
     2281 ttgaatatga tatttgtgat ttataatgga aacaacacag cctgccatga aggagcttaa
     2341 acacatgttt gtcgaacaga ctgaaaaaac acccctggtc gatttcaata atcttacagg
     2401 tgaactgata ctttcaggaa gatcaatccc aatcaatgct cccagaatct ttgaaccaat
     2461 tctcgaatgg gtaaacaatt atataaaaga tccaaggcca acaacaaatc taagattaaa
     2521 tcttgaatat ttcaatacgg catcttccat atggctcgca aaaatagtaa aggcacttac
     2581 aaacattgaa aaacaggatt gtgttctttt ccttcatgtt tatttctccc ttgaggattt
     2641 tgatgatata gatgatatta aagatgatct cagtccggta atcgatgttg taagcaattc
     2701 aaaggtcagt gtgggactta aaatctatgg cactgatgaa gcgggaaaaa taatgaagga
     2761 gtcaatgatt ttcatctagc agtccccccc tgcgagtaat acttctacaa aaacaaccac
     2821 ttttcgtttt tttgacctac ctttattctg gtaattcagt aagatttaac tatgccagca
     2881 gtgtggtttg ttttgctctc aattcttaca gtcatggaga catattctca gccaggatcg
     2941 gccgacaggt accccgctgt ggcgggaagg ttttattctg ccgataagga gactctcaga
     3001 aatgatatct caaagctatt tgagaactgt aaaaaacctg tggatcataa acatgtaagg
     3061 gctatcatca gtcctcatgc cggttacatc tactcggggc gggtagccgc ttcggctttt
     3121 tccgccatcg gaaagaaaac cgtatacaaa aatatattta tcattggctc aagtcatata
     3181 atggcctttg aaggagcatc tgcatattat accggcgact ttgttacccc tctcggcaag
     3241 atcagggtca accgggaaat agcaaattcc ctcagaagca acaggttgtt caatcatccg
     3301 gtcagcgccc acctgcagga gcacagcctc gaagtccaga tcccattcat acagtattat
     3361 ttaaccggcg atcccatgat tgtgcctgtt attatcggaa caaataacat aaattcgata
     3421 aagaagattg cagaggcgct taaaccatgg ttcaattccg ataacctttt tataataagc
     3481 agcgattttt cccattatcc cggatacaaa acagcctgcg aggttgataa ggcaacagct
     3541 gatgcaatta tttccgggga tccgcagaat ttcctcaata cgctgaaaaa aaactcgctc
     3601 agaaatgaac cggggctggc aacaagtatg tgcggctgga cgtctggcct gcttcttatg
     3661 tacctggccg aacaggaagg aaatcttgaa ttcagcaagg tcgattactg taattcgggt
     3721 gactcaaagt acggcaacaa ggatgaggtt gtgggttaca atgcaataat acttgccgaa
     3781 aaaaaaattc ccggacattc aacgggaagt ctttatgacg aaatatcctt cacggccagg
     3841 gaaaaggaag agcttattgc cctcgcaaga aacaatataa gatccctgct caatgataga
     3901 aaaatgataa ccgtggatga gaataagctg ccggcaatct tcaaacaacc tctgggtgct
     3961 tttgtaacac tgaagattga taacgtgtta agaggctgta tcggaaaatt catctcatcc
     4021 gatccgcttt tcagggttgt tcttgaatcc tcaaagtcgt cagcctttga agatcccagg
     4081 ttccctcccc ttaccaaggg agagtttgca aaaaccgata tcgaaataac ggtcctgggc
     4141 ccgctgaaga aaatcactga cataaatgaa ataatcctgg ggaaacatgg catttatata
     4201 aaaaaagatt ttcactctgg aacaatgctt ccgcaggttg caaaggagaa tggatggacc
     4261 gttgaagaat tccttggtta tacgtcgcgg gataagacag gtattggttg ggacggatgg
     4321 aaaaaggccg acatttttgt ctatgaagga atcgttctcg atgaaatcaa aaagtaacca
     4381 tgtattatgt tactccagtt ttattaacaa taattgcact gtactgcctc agttactttt
     4441 ttttcaggaa cagaaaattc acgattcaat tacacttcag attctggaat cttataattg
     4501 ccgcattatt tctgcttact gctgcaaccg gcctgctcag gattttgcat acagctcctg
     4561 aatgggatgc tgctttttta aaatccatgg cagggcagca tgttgtatta ggcatggctt
     4621 ttatttcaac cggcttgctt cattttttcc tgcattattc ccgtttccgg aatctgttca
     4681 agaaacggga atcagatgct ccaaatgagt tttattgccg cacacagcga acccgtggtg
     4741 caatcggagc aaatcttttt gcagtaggct ttgtaagttc tgccattcaa ttgcttctgc
     4801 taagggaaat cctgaatatt tccggtggct atgaactgat tgccggtacg ttcctgggat
     4861 cgtggctaat ggcatcagct gccggttcgg caatagctcc aaaaacatcc ataaccaata
     4921 ttctgagcat caatctattg tttttctcag gacctgtaat atcagttttt ttattaatta
     4981 tactttccag actttttctt aatccgggtg aaacaccttc atatcttgcc agcatcatat
     5041 ttaccctcat tgtccttgta ccgttctgtt tcatttcagg ttttaccttt atcaaactgg
     5101 tttcagaggc ttcatcaata aaggatttca ggccggggag atcatttgca gtcgaaacat
     5161 ccggaggaat tgcagcaggt atattaattt catttgcaag ccatggcggt ataaacaatt
     5221 accaggctat tatcctgata atcatttcgg ggttttccta taccgttatt tcacaatccg
     5281 gcctgaacag acggaacttg atgctcaccg ggataatcgt cctggccctg tctgcaggag
     5341 tgatcatatc agcacccgac aaatatttca ggcagcttct tctcaggggt cttacggtaa
     5401 cagattcatc agatacacct tacgggaata tcacccgggg gatgtacggc gaagaaaaaa
     5461 gcacttacta cgatcacagg cttctttttt acagcgatga tataatagaa cgtgaagaag
     5521 acatacatta tcccatgctt caacttgata atcctgaagc ggtactcctt gtctccggat
     5581 cattatatcc acaccttggc gaaataaata aatacaaggc aaaaagggtt gtatacgttg
     5641 aacgtgatcc ggccctgatc aaggctgaaa gcagcactga accgtttaag atacctgatc
     5701 tgctggttga aaacaaagat gcatacacat tcatcaaaaa tgcaggggag aagtttgatg
     5761 ctgttatttt actccttcca cctccttctt ccctcctgct taaccgtttt tatacttatg
     5821 aattcttcat gcatgtcaaa aaggtactga agccggaagg cgtattttcc tgtgcaccgg
     5881 gcattaatcc cgattatctg aatatcgagt cggtactttt tttctcatca atctataaca
     5941 gccttaaagc tgtattcaga tatgtcgttc ccgtcggtgg aaataagtta tatcttctgg
     6001 cagcagacaa ggaaatatca atatcattct gtgagctaac ggaaaaaaag ggaataagca
     6061 atctttatgt agggcctgat tacctggctg acgatctgac agaattgaaa acagaccaga
     6121 ttatttcagt gattgatgag tctgtaaggc tgaaccatat tctaaccccg gttgcaacct
     6181 tttattttca ggcttacaat atcagcaaga gtatcaatga aaaactaccc ctgatcattt
     6241 tgctggcctt acttttctta ttgcccctcc tgacggtgag aaggacaaat atgctaatgt
     6301 atttcagttc ttctgcgctg gcaggctatg agataatcct gctgctagtc ctgcagtctt
     6361 cggtcggtaa catgtatcag gttacaggcc tgatacttgc cggcctgctc tcagggctgg
     6421 ccgtcggctc cgggctcagc ataccctttc ttgaaagccg cacttaccgg atcaagtcgt
     6481 ggcttctgat aatttgttat ctggcaatgt attttgcaat aaaaaggctg gttgactcag
     6541 atagcaatgt gtttgtgact gtggcattga taatttcggg atttattccg gcagccatca
     6601 ccggcaacct gttccgggag ttcactcaaa ccgccatggt tccgtcagtg ccgtcaccag
     6661 tatacagtgc cgaccttgcc ggatcggcgg ccggatgtat tgtgttttca ggctttgcag
     6721 ttccgttaat tggtataggg ccgtccctgc tcatctttcc tgtactggtt ctcacgggat
     6781 atctttttgc tttgaaagcc agaaaatgac atcatttaat aactttgcaa acagaggtaa
     6841 gtattaagag aatttcatat ggaaaatcat cgtaacatga taagcaaacg caactttgta
     6901 aaaaggtgtg cagctttatc cacaggtctg atctgtgttc cgtacggcag cctcctgtct
     6961 gaaatgtctt ctgtgcaggc aggcatctac aaaaaggaag ccatgtttca ggaagaaaca
     7021 ccgggaggga tcatgtgcat gatctgtcca aacggatgtg ttctcaggga aggggaagtc
     7081 agccggtgca gaagcaggaa agtcagaaaa tcaaagttat atacgcttgc ttacggaagc
     7141 ccgtgttccg tgaatgttga tccgatggag aagaaacccc tgtaccattt tttgccgggg
     7201 tccagggcat tctcgatagc tgccgccgga tgcaacctgt catgtcttaa ttgccagaac
     7261 tggacaattt cgcagaccag ccctgaaaaa accaggaatt acgacctgat gcccgacaag
     7321 gttgtggagg agtgtatcag caggggttgc aaatccattg catatacata ttcggaacca
     7381 gtaacattct atgaatatgt ttatgaaacc gcaatcgttg caagaaaagc agggataaag
     7441 aacattgtaa aatcgaacgg ttacatcaat cctgagccgc ttaagaaact atgcagcgtg
     7501 tttgacgcag caaatattga cctgaaatct ttcaatgaaa gtacctatct taaacttacc
     7561 ggaggcaggc ttcaacctgt tcttgactca ttgaaaatct acagggatat gggtatctgg
     7621 ctcgaaataa ccaatcttgt ggttcccaca tggtccgaca atcttgatga gataaggaaa
     7681 atgtgcagat ggctgagtga caacggattt aagaaggttc ccattcattt cagcagattc
     7741 tttccagttt ataaacttga gcaactccct cccaccccta tcgaaaccct taacagggct
     7801 gcaagcattg cctctgaaga gggcttgtta tatgtttaca ccggtaatgt tccgggaaat
     7861 gaactttcgg atacaaaatg tccgtcatgc aagaagccgt tgatctcccg ccagggattc
     7921 cgtgtaacct caaacaccat aagggatgga aaatgtgaca gctgcggccg gatcatcgat
     7981 ggtgtgtgga actgatccct tgaaatgatc tgattaaata aaaataaccc tgcagatgaa
     8041 ccagactgaa gccaggaagc gtattgagaa gctgcgccgt gaaattgagg aacacaacag
     8101 gaattattac gtattcaatg atcctgtcat ttccgatttc gaatatgatc tgctcataaa
     8161 tgaactcgag acccttgaga aacaatatcc tgaattttac agtgaggagt cgccgacccg
     8221 gcatgttgga agtgacctga cacgtgagtt ccggcaattc gaacataaat atccgatgtt
     8281 gtcgctgggg aacacataca gtatagagga aatcagggat tttgacagca gggtaaggaa
     8341 atcagtaatg ggtgaaataa gctatgtttg tgagcttaaa ttcgatggtg cctcgataag
     8401 catatcctat tcaggaggat caatggaaaa agccctgacc aggggcgacg gtaccagggg
     8461 agatgacgtc acagaaaata taacgaccat aaaaagcatt ccgggcagga tcggttcaga
     8521 cgctgtccca gcttccttta tagcaagggg ggaaatactt atgcccagga gtgtattcaa
     8581 ggagctgaat gaagaaaggg aaaaggaagg cttaaatctg ttcgctaatc cccgcaatgc
     8641 tgcgtcaggg acattgaaaa tccttgaccc atccctggtt gcagccagaa agctggattg
     8701 ccttttctat tccctgctgg gcgatgatct gccggcagac aatcattatg ataatctgaa
     8761 aaaactgcag gaatggggat ttcccgtatc tgacagttac aggctctgca ggaacctgga
     8821 tgagattatc ggattcattc agttctggga aaccaggcgc aacgatctcc cgttcgacac
     8881 agacggggtt gtcatcaaag taaattccct cgatcaacag ctgcagctgg gtttcactgc
     8941 aaaatcgccc aggtgggcaa ttgcctacaa atacaaacct gagcgggcat tgacaaagct
     9001 tttatttgta tcctaccagg tcggcagaac cggcactgtt actcccgtgg caaaccttga
     9061 acctgtccag ctggccggca ccactgttaa aagagcctct ctccacaatg ctgaccagat
     9121 ttcacttctc gatctgcatg agggtgatat ggtttatgtg gaaaagggcg gagaaataat
     9181 accaaagata gtcggtgtgg acaaagcttc gaggtcagat acgagcatgg cgatcagctt
     9241 tattgaagaa tgcccggagt gcggtacacc acttgttaga aatgagggag aagctaatca
     9301 ttactgcccc aacatactcc attgtcctcc ccagataaag ggcagaataa gtcatttcat
     9361 cagccggagg gcaatgaata ttgaaggact gggggaagaa accacggatc tcttgtacac
     9421 cagggggctg atccggaatg ctgcagacct gtacgatctg aaagccgggg agctggcatc
     9481 gcttgaaggg ctaggagaaa aatccgcatc caatataata agaagcattg aagattcaag
     9541 gaacgtacca tattacagag tactgtttgc actggggatc aggcatgtag gagaaacggt
     9601 tgcaaagaag atttcggtca ggttccggtc catcgacaaa ctgatcagtg ccggtatcag
     9661 cgagattaca gaag
//
LOCUS       DAOOYG010000096         9645 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_7700_length_9645_cov_7.379249, whole genome shotgun sequence.
ACCESSION   DAOOYG010000096 DAOOYG010000000
VERSION     DAOOYG010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7700_length_9645_cov_7.379249"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PK288_08530"
     CDS             <1..979
                     /locus_tag="PK288_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM19010.1"
                     /translation="AMLCGMTFDRDGNLWMVQGHSASGVKVLTADGKWIIFPCQINTR
                     VPGDIIAAPDGNKWIIQPKLNSLFIIDDNNTPGLLSDDRYLQLPVRDTEGNYFSVFSL
                     NKDLEGNIWAGTDKGPLVYFNTENIFEKSPAAYRIKISRDDGSGLADYLLGTETITSI
                     AVDGANRKWLGTSGSGAYLLSPDGGKVLKTFNKSNSPLFSDSIATIAVDNRTGEVWFG
                     TAGGILSVRETATSGGQEFTGVYSFPNPVREDFSGNVTITGLMKDTNVKITDVSGNLV
                     FETTSTGGQASWDLKTFNGKRVVTGVYLVFCSSADGSQSCTTRILVISR"
     gene            complement(980..2383)
                     /locus_tag="PK288_08535"
     CDS             complement(980..2383)
                     /locus_tag="PK288_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPM19011.1"
                     /translation="MIAADNEELIRRIARLARQNQEQDDLIKKLEKQIENLTRQLEKL
                     KNGQIPQDSSLVQAVKEEAREKSLKFDMATVLFAELRCSDPGLEEHDSFSYMDELDEI
                     LMEFGAIAERSGIVKIKTIGDTFMCAGGIPEKNITNPVIVVLAAVEMKNFLDRFEARK
                     RGNGRKIWQLKTGIHTGPVLATVTGKKKISYEIKGDTVNNASRIVTVSDDQSILISIM
                     TCELVKELFVCEYSGRLPVKYKGHLDLFGVTGLKPGLSVDAKGMIPNDLFRIKFGLIQ
                     FTDIQEMILDKLERELPPFLFYHNVKHTVDVVTEVELIGWAEGCSDEEILLLKTAALF
                     HDAGHIVSYDEHEMHSARMAEEILPRYNYTSAQIERICSIIMATRLPPEPADLLEEII
                     CDSDLDYLGRSDFIPVSNMLFEELRARNKMTEINEWNKLQVKFISGHQYFTRTARSLR
                     EVNKQLQIERIRSLIHD"
     gene            2444..3169
                     /gene="recO"
                     /locus_tag="PK288_08540"
     CDS             2444..3169
                     /gene="recO"
                     /locus_tag="PK288_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPM19012.1"
                     /translation="MLLKTKCIILHHTRYSDSGLILQAYTREAGRLSFMVKGMRSKGS
                     GKHNVLFQTMSMLEVILYYKESGSIQLLKEFSVSYIPSGIFTDIRKSSIAVFIGEVLS
                     QVLKEETQNYELYDHLENSLKYLSESSAGFSNFHIAFLIGLSSYLGFEPGKRTSPEKK
                     FFDLVDGRFVTMPPAHGNYANEEISGILASFFSASYGSMSSIPLTGSLRNEILETIIK
                     YYSIHLPLFRKIKSLQVLKEIFG"
     gene            3230..4762
                     /locus_tag="PK288_08545"
     CDS             3230..4762
                     /locus_tag="PK288_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPM19013.1"
                     /translation="MPFLPSIVKLLNANRIKQIEHFRKHPVDVQNQVLLRIIETAIPT
                     EWGRKYSFQSIRSYTDFQSRVPVTNYEELLPYIERMRRGEKNIAWPGEIKWFAKSSGT
                     TSSKSKFIPISKESLWGCHYQAAKDILTLYAVNNPETKVFTGKALTLGGSNRINMSDN
                     KSIYGDLSAVLIWNAPFYIEFVRTPRQEIALIEDFEQKLELITKTTVSQNVTSLSGVP
                     SWYLTLIRHVLAYTGKSNLLEVWPNLEVFFHGGISFTPYREIYRKLIPGGQMHYMETY
                     NASEGFFGIQDDPGRSDMLLMLDYGIFYEFIPADRLKSGEAQPCPLTEVKEGVNYAIV
                     ISTNGGLWRYLIGDTIVFTSTDPYRFRISGRTRHFINAFGEEVIVENADKALEAACKG
                     TGAIITDYTAGPVYMGETSRGTHEWLIEFEQSPCDTDKFTILLDEELKALNSDYEAKR
                     FKDINLVRPIVRPVPVGTFKLWLKENNKLGGQNKIPRLSNDRDFLEEIYKYAGFGKDP
                     AR"
     gene            4937..5551
                     /locus_tag="PK288_08550"
     CDS             4937..5551
                     /locus_tag="PK288_08550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPM19014.1"
                     /translation="MHIAIAGNIGSGKTTLAGLLAKHYGWTPHYEDVDDNPYLNDFYE
                     DMQRWSFNLQIYFLNSRFSQIIEIRKSNQSIVQDRTIYEDAYIFAPNLHSMGLMSTRD
                     FENYTALFNLMTSFLQPPDLLLYLRASVPTLVSQIQKRGREYESSIRLDYLKRLNERY
                     EAWVQSYKLGKMLIIEADHYNFPNNNDHLSEVIDKINAELHGLF"
     gene            complement(5698..7164)
                     /locus_tag="PK288_08555"
     CDS             complement(5698..7164)
                     /locus_tag="PK288_08555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPM19015.1"
                     /translation="MKKLNLLIPFTVFSTVALIVISCAINPVTGKRQLMLMSESQEIA
                     LGISYNPQVLATFGVYDDEELQKFVQARGTELGKISHRPNLEYHVKVVDSPVVNAFAV
                     PGGFIYLTRGIMAQLNNEAEFMGVLGHEMGHITARHTVSQQSKQQLGTLLLIGGMIAS
                     EKFAQYAEYAMQGMQLLFLRFSRDDEREADRLGVEYSSKVGYDAHKMADFFRVLQKMS
                     MAESHGGIPTFLSTHPDPGDRYNAVNQKASEWQSTLSMPEWKVNQENYLRLIDGIVYG
                     DDPRQGYVDGNVFYHPDLKFRFSFPSGWKLENSPIQVTMSPPDGKALILFSLSQQKTL
                     ESAVDSTMAQYGLSIMGSKRASVNGLQAIIVQSKQVSRDQATGATSTTLVLSYFILYN
                     NLIYVFHGVSAEADFNTFNGVFNSSMSSFARLTDASRLNVKPQRVHVVRVQRSGTLAD
                     AFNYYRVPRDKFNELALLNDLELNSSLPAGRLIKIIGE"
     gene            7649..9325
                     /locus_tag="PK288_08560"
     CDS             7649..9325
                     /locus_tag="PK288_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM19016.1"
                     /translation="MKRREILKTLSAIPVAGGMMSIEPIMASVSQKKPASDAGLLSNP
                     PPGALALGPQIYQSIGVEPMINCRGTFTIISGSIELPEVQKAVDYASRHYVHIDELAM
                     AVGKRLAAITGAEWGMVSSGCAAGLKHVTVACITDGDPEKLVRVPDLTGFTKTEVIVP
                     GYSRNQYDHAIRNAGIKMITVNSMEEFRKAVSSRTAMIYLFSAPREYYEGPLSIENIA
                     PIARENNIPILVDAAAEILTIPNDHLKRGATVVAYSGGKALRGPQGAGLLLGSKDLLF
                     STWQASSPHHGPGRDNKIGKEEHIGMLAAVEAWAKRNHPEEEKTWISWLEYISRKVTT
                     INGVTAKISEPQGVDNRSARLSISWDPEVLNITGEEVADDLLNNKPRIALGGGRTNPR
                     DRTASISISAQMMQPGDEKIVADRVYKILSQKHEKKVVVLDPPAANLVGHWDVSIDYY
                     SGKGEHKLFLEKQEGNEIKGTHKSTFSLLDIAGQIDGKKVRLQSFYSDNAETVPFTFD
                     GVIDGDTFSGVLHLGEYRSANFIAKRSEQRQRPFFSIRVPSYGGRKSDSW"
BASE COUNT         2656 a         2254 c         2248 g         2487 t
ORIGIN      
        1 ggccatgcta tgcggaatga catttgacag ggatggcaat ctctggatgg tgcagggcca
       61 ttcagcctct ggggtaaagg ttctcacggc tgacggaaaa tggatcatat ttccctgcca
      121 gatcaatacc agagttccag gcgatataat tgccgctccg gacgggaaca aatggattat
      181 ccagccaaaa ctgaacagcc tgttcattat tgatgataac aacactccag ggctgttatc
      241 agatgacagg tatcttcagc tccccgtcag ggacactgaa ggtaattact tcagtgtctt
      301 ttcattaaac aaggatctcg aaggaaatat atgggcaggg accgacaagg ggccgcttgt
      361 ttatttcaat actgaaaaca tcttcgaaaa aagcccggca gcctacagga taaagatctc
      421 aagggatgat ggttcggggc tggctgatta tctgcttgga acagaaacca tcacttccat
      481 tgctgttgac ggcgctaaca gaaaatggct tggaacatca ggttcaggcg cttatctatt
      541 atctcctgac ggcggcaagg ttctgaaaac cttcaacaag agcaattctc cgttattttc
      601 tgattcaatt gcaactatag ctgtggataa ccgtaccgga gaggtctggt ttggtaccgc
      661 aggaggcatt ctttcagtga gggagacagc tacgtccggc ggacaggaat tcacaggggt
      721 ttattcattt cccaatcctg taagggaaga tttttccggt aatgttacaa ttacaggatt
      781 aatgaaagac acaaacgtaa aaatcactga tgtgagcggt aatcttgttt ttgaaaccac
      841 ctccaccgga ggccaggctt catgggatct gaaaaccttt aacggaaagc gtgtagtaac
      901 gggggtttat ctcgtttttt gcagcagtgc tgacgggtca cagtcgtgca caaccaggat
      961 cctggttatc agccggtgat cagtcatgta tcaggctcct gatcctctct atctgaagct
     1021 gcttgttcac ttcccgcagg cttcttgccg tccttgtgaa atactgatgt cccgagatga
     1081 atttaacctg caatttgttc cattcattga tctccgtcat tttattcctg gccctgagtt
     1141 cttcaaaaag catatttgaa acagggataa aatcacttct gcccaggtag tcaagatctg
     1201 aatcacagat aatttcctcg agaaggtcag ccggttcggg agggagcctg gttgccatta
     1261 ttatcgaaca aatgcgttct atctgggcgg atgtgtaatt gtatcgcggc agaatttctt
     1321 cagccatcct tgcactgtgc atttcatgtt catcatagga aacaatatgt cctgcatcat
     1381 gaaaaagagc tgctgtctta agcaaaagga tttcctcatc agaacagccc tctgcccagc
     1441 ctatcagctc cacttccgtt accacatcaa ctgtatgctt cacgttgtga tagaaaagaa
     1501 acggcggaag ttccctttca agtttatcaa gtatcatttc ctgaatatcg gtgaactgga
     1561 taaggccgaa ttttatcctg aacagatcat tgggaatcat tccttttgcg tcaaccgaca
     1621 atccgggttt caaccctgta accccgaaaa gatcaagatg acccttgtac ttgaccggca
     1681 gcctccccga atattcacaa acaaacaatt ctttaaccag ttcgcaggtc attatcgata
     1741 tcaatatgct ttgatcatct gatacggtca caattctgct ggcattgtta actgtatctc
     1801 ccttaatctc ataactgatc ttcttcttgc cggtcacagt tgccagaacg ggtccggtgt
     1861 gaatgccggt cttcagctgc cagatctttc tgccattccc tcttttccgg gcctcgaacc
     1921 ggtcgagaaa gtttttcatc tcaacagcag ccagaactac aatgacaggg ttggtaatgt
     1981 tcttttcagg aatgcctccg gcacacatga aagtatcacc aattgttttg attttcacaa
     2041 tccccgatct ctctgctatt gcgccgaatt ccatgaggat ctcatcaagc tcatccatat
     2101 aggaaaagga atcatgttct tcgagaccag ggtcggagca ccgtaattcg gcaaaaagca
     2161 ctgtagccat atcgaacttc agggacttct cacgcgcttc ttctttaact gcctgaacca
     2221 gagaactgtc ttgaggtatc tgtccatttt tcagtttctc caactgccgg gtcaggtttt
     2281 caatctgctt ttcgagtttt ttaataagat catcctgttc ctggttttgc ctggcaagcc
     2341 tggctattcg tcttatcagt tcctcattgt ctgcggcgat catgatcctt ttgattggca
     2401 ggtaaatata ataattttat atttgagtct gacttttgct gacatgctgc tgaagacaaa
     2461 gtgtattata cttcatcata ccaggtattc agattccgga ttgattttgc aggcttacac
     2521 ccgtgaagca ggtcggcttt cgttcatggt gaaaggaatg agaagcaagg gatccggaaa
     2581 gcacaatgtg ctttttcaga caatgtcgat gctggaggtg atcctttatt acaaggaatc
     2641 aggcagcatt cagcttctta aggaattctc ggtttcatac atcccttcgg ggatattcac
     2701 cgacataagg aaaagttcca ttgccgtatt cattggcgag gtcctcagcc aggtattaaa
     2761 ggaagagact cagaattatg aattgtatga tcatctggaa aactctctga aatacctcag
     2821 cgagagcagc gccggctttt caaacttcca tattgcgttc ctgatcgggc tcagcagcta
     2881 tcttggattt gaacccggta aaagaacttc cccggagaag aaatttttcg atctcgtcga
     2941 cggcaggttt gtgacaatgc cgccagccca cggaaactac gccaatgaag agatatccgg
     3001 cattcttgcc agtttttttt cagcctccta cggatcaatg agcagcatac ccctgaccgg
     3061 atcactaaga aatgagatcc tggaaacaat aataaaatat tattccattc accttccgtt
     3121 gttcaggaag ataaagtcgc tgcaggtcct gaaggagatt ttcggttgat gtgcgattta
     3181 tccatgggat atttttaact atctttagca ttcaagcccc agtgttttta tgccattttt
     3241 gccttcaata gtaaagttgc tgaatgccaa ccggataaaa caaatagaac atttcaggaa
     3301 acatccggtg gatgtacaga accaggtcct tctcaggata attgaaaccg ccatccccac
     3361 agaatggggc agaaagtatt cttttcagtc gatacgatcg tatactgatt ttcagtcgcg
     3421 cgttccggtg acaaactatg aggaactatt gccatatatt gaaaggatgc gaaggggaga
     3481 aaagaatata gcctggccgg gagaaataaa atggttcgca aaaagttccg ggacaacctc
     3541 ttcaaagagc aagttcatcc ccataagcaa ggaatcactc tggggttgtc attatcaggc
     3601 tgccaaggat atactaaccc tgtatgccgt taataatccg gaaacaaagg tatttacagg
     3661 caaggcgctc accctgggcg gcagcaacag gataaacatg tcggataaca agtcaatata
     3721 cggtgacctt tcagcggtgc tgatctggaa tgcgcctttc tacatagaat ttgtaaggac
     3781 accccggcag gaaatagccc ttattgagga ttttgagcag aaacttgaac tgatcaccaa
     3841 aacgaccgta agtcagaatg tcacaagcct gtcaggtgtc ccgtcatggt acctgacact
     3901 tatcaggcac gtgcttgcgt atacgggaaa atcaaatctg ctcgaagtat ggcccaacct
     3961 tgaagtgttc tttcacggcg ggatcagctt caccccctac agggagattt acaggaagct
     4021 tatcccgggc ggccagatgc attatatgga aacctacaat gcatccgaag gttttttcgg
     4081 tatacaggat gaccccggca gaagcgatat gctgcttatg ctcgactatg gaatatttta
     4141 cgaattcatt cctgccgaca ggcttaaatc aggcgaagca caaccatgcc cgcttactga
     4201 agtaaaggaa ggcgtgaatt atgcaattgt catatcaacc aacgggggcc tctggagata
     4261 tcttattggc gacacaattg tctttacatc caccgatcct taccgattca gaatatccgg
     4321 acgcacacgt cattttatca atgccttcgg cgaggaagtg atcgtggaga atgccgacaa
     4381 ggcccttgaa gctgcatgca agggtaccgg tgcaataata acggattata ctgccggccc
     4441 ggtctatatg ggagaaacct caaggggaac tcatgagtgg ctcatcgaat ttgaacaatc
     4501 accttgcgac actgataagt tcacgatact cctcgatgaa gaactgaaag cgctgaactc
     4561 agattatgaa gcaaaaaggt tcaaggatat caacctcgtc aggccgatcg tcaggccggt
     4621 gccggtcggg accttcaaac tctggctgaa ggaaaacaat aagctgggag gacagaataa
     4681 gatcccaagg ctttccaatg acagggactt tcttgaagaa atatacaaat atgccggatt
     4741 cggaaaagat cctgccagat agtggcgcgg gggcattcct ggtgaaaacc ggatgcagta
     4801 ttacgggaca tattaataaa cgccggtatc atgctgcata ttagccgttt gtatggcatg
     4861 cactataaac ctggaaacat aaattcaaga ctttttttta ttatttttac aaccggaaaa
     4921 aaacaagcat tcaaccatgc atatagcaat tgcaggaaac atcggatcag gaaaaacaac
     4981 cctcgcgggt cttctggcaa aacattatgg atggacgccc cattatgaag atgttgatga
     5041 caatccatat ctgaatgatt tttatgagga catgcaacgc tggtccttta atctgcagat
     5101 atattttctt aattcaagat tcagccagat cattgaaata aggaaatcga accagtcaat
     5161 tgtgcaggac aggaccattt acgaagatgc atacatattt gcccccaacc tgcactccat
     5221 gggattgatg tcaacccgcg actttgaaaa ctatactgcc cttttcaatc tgatgacatc
     5281 attcctgcag cctcccgatc tgctgttgta cctgagggca tctgttccga cgcttgtaag
     5341 ccagatccag aaaagaggca gggaatatga aagctccatc aggctcgatt acctgaagcg
     5401 gctcaacgaa cggtacgaag cctgggttca gtcatacaaa ctgggaaaga tgctgatcat
     5461 tgaggcagat cactataatt tcccaaacaa taacgaccat ctgagtgaag tgattgacaa
     5521 gatcaacgca gaactccacg ggcttttctg actctcatct tacccgtgag ccggaaaacc
     5581 tatggcagat caccggggga aatccacagc gaactgggtt gctatatatc accggcacct
     5641 gtctgacagt ggcagtaaat atgaattccg gggtttttgc aaggcttaaa agaataccta
     5701 ttctccgatt atttttatca gtcttcctgc cgggagactg ctgttcagtt caagatcatt
     5761 aagcagggca agctcattga acttatcacg cggcaccctg tagtagttaa atgcatcggc
     5821 gagggttccg ctgcgctgaa ccctgacaac atgaaccctc tgcggtttta catttaaccg
     5881 ggaggcatcc gtcagtcgtg cgaaactcga cattgatgag ttgaacactc cattgaatgt
     5941 gttaaaatcg gcttctgccg aaacaccgtg aaaaacatag atcagattat tgtacaggat
     6001 gaaatatgaa agcaccagcg ttgttgaagt tgcgccggtc gcctgatccc tggaaacctg
     6061 cttcgactgg acaataattg cctgcaggcc gttgacggaa gcccttttgc ttcccattat
     6121 gctgaggccg tattgtgcca ttgtactgtc gaccgcagac tcaagtgttt tctgctgtga
     6181 aagggaaaac aggatcagtg cctttccgtc cgggggactc atggtcacct gtatgggtga
     6241 gttttccagt ttccatcccg aaggaaagga gaacctgaac ttcagatcgg gatgatagaa
     6301 tacatttccg tcaacatatc cctgccttgg atcgtccccg tacactatcc cgtcaatcag
     6361 gcgaaggtag ttttcctggt ttaccttcca ttcaggcata ctgagtgtgg attgccattc
     6421 cgatgctttc tggttaacgg cattataccg gtcgcccgga tcgggatggg tcgacaggaa
     6481 tgtgggaata ccgccatggc tttcggccat gctcattttt tgtaggaccc tgaaaaaatc
     6541 ggccatctta tgggcatcgt atccgacctt tgatgaatac tccacgccca gcctgtcggc
     6601 ttcacgctca tcgtcacggc tgaaacggag aaaaaggagc tgcataccct gcatggcata
     6661 ttcggcatat tgtgcaaact tctccgaagc gatcatcccg cctatcagca gcaaagtgcc
     6721 aagctgctgc ttgctttgct ggctgacagt atgacgcgcg gtaatgtgac ccatttcatg
     6781 tccgagcaca cccatgaact cagcttcatt gttaagctgg gccataatac cccgggtcag
     6841 gtatatgaat cctcccggaa ctgcaaatgc attcaccacc ggtgagtcaa caaccttgac
     6901 gtggtattcg agattgggac ggtgagatat ttttccgagt tctgtgcccc tggcctgaac
     6961 aaatttctgt aattcctcgt cgtcataaac cccgaaagta gccagcacct gtggattata
     7021 tgaaataccc agggcaattt cctgcgattc cgacatcagc atcagctgtc tttttccggt
     7081 aaccggattt atggcacagg aaatcacgat caatgcgact gtgctaaaaa cagtaaacgg
     7141 gataagcaaa ttcagctttt tcattgcgac tgacagtttt gaacttttta accttctaaa
     7201 ataacaataa aacttaaata aagcctatcc gcagcgaatt gcacaagcca aaaagcgtat
     7261 ggcatcacgc gactccacga tttaagttac aagtatatcc tgtaataatc ctgatctccg
     7321 attatccgga gttttgccac cggttctgaa tctttcaggc cggattatgt accatgactg
     7381 atctcaaatc ggcaatgtcc ggtgcatagg gcatgatcag cccggcaaat ggccgggatt
     7441 tataagtttt taacaattaa tggcggcctt attctttttt attatctact tttatagtta
     7501 ggaacctcac aggacgcctg ttattttcca gggtcaggcg ggccggtgat ttcggtgaag
     7561 gctggaaaat aattaaagaa tattaaacat gcgggttctc ccatttggcc ggacaggcat
     7621 gttaccctaa aactaaaaaa ttttaaagat gaaacgcaga gagattttaa aaacattatc
     7681 agcaattcct gttgcaggcg gaatgatgtc aattgagccg atcatggcat cagtcagtca
     7741 gaaaaagccg gccagtgatg cagggctcct gtcaaatcct cctccgggag cacttgccct
     7801 gggtccccag atctatcagt cgattggtgt ggaaccaatg ataaactgca gggggacttt
     7861 tacaatcatc agcggatcga ttgaactgcc cgaggttcag aaggcagttg attatgcatc
     7921 caggcactat gtacacattg atgaactggc aatggccgtc ggcaagaggc tggcagcaat
     7981 caccggggct gaatggggaa tggtatcctc aggttgcgcc gcaggtctga agcatgtaac
     8041 ggtagcctgc atcaccgacg gagatccgga aaagcttgtc agggttcccg atcttacggg
     8101 ctttacaaag actgaggtaa tagttcccgg atattccagg aaccagtacg accatgcaat
     8161 aagaaatgca gggatcaaaa tgataaccgt aaacagcatg gaggagttca gaaaagcggt
     8221 aagttcacgc accgctatga tttatctgtt ctcagcacca agggaatatt atgaggggcc
     8281 cctgtccatt gaaaacatag cccccatagc cagggaaaac aatattccaa ttctcgtcga
     8341 tgctgcagcg gaaatcctta caatcccaaa tgatcatctg aaacggggtg cgacggttgt
     8401 tgcctacagc ggcggcaaag cactcagggg gccccaggga gcagggctcc tactgggcag
     8461 caaggatctc ctgttctcaa catggcaggc cagctcacct caccatggcc cgggaaggga
     8521 caacaagatc ggaaaggagg aacatattgg tatgcttgcg gctgtggaag cgtgggcaaa
     8581 gagaaatcat ccggaagagg aaaagacatg gatatcatgg ctggaatata tatccaggaa
     8641 agttaccacg ataaacggag taactgcaaa gatttccgaa ccccagggag tcgataaccg
     8701 aagtgcccgc ctgtccattt catgggatcc ggaggtcctg aacattacag gggaggaagt
     8761 ggccgacgac cttttaaaca ataaaccgag gatcgccctt ggcggcgggc ggaccaatcc
     8821 cagggatcgc accgcttcaa tatccatcag tgcccagatg atgcagcccg gcgatgaaaa
     8881 gatcgttgcc gatcgggttt acaaaatact ttcgcagaaa catgaaaaga aggtggttgt
     8941 acttgacccg cctgcagcaa acctcgtcgg tcactgggat gtgtcaattg actactattc
     9001 cggcaagggt gaacataagc ttttcctcga aaaacaggaa ggcaatgaaa taaagggcac
     9061 ccataaaagt accttttccc tgctcgatat tgcaggacag atcgacggga aaaaagtcag
     9121 gctccagagc ttttactcgg ataatgccga aactgttcct ttcactttcg acggcgtaat
     9181 tgacggcgac accttctccg gtgtgcttca tctcggggag taccgctcgg ccaatttcat
     9241 cgcgaaacgt tcggaacaga ggcaaaggcc gtttttcagc atacgtgtgc cttcatacgg
     9301 aggaaggaaa agcgattcat ggtaaaaccg gtgacccaat tgcgtgaact tccgtaagcg
     9361 ggcgttaagt ccatgacaag cctgttgcct gccatggccg cagatcgtgt ttaagcataa
     9421 tgctcagcag aacaaataaa gggtgccttt aaaagggcat cctttttgtt ttcaaggtag
     9481 cttttcattt catttgtgag tgcgagtgcg agtgtgggtc gtgaggaaaa aagagtgaga
     9541 gtgttcgttt gtgcttcgca ctcgctctca ctctcactct tttttcagct ccccagctag
     9601 gacttgaacc taggaccccc tgattaacag tcaggtgctc taacc
//
LOCUS       DAOOYG010000097         9490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_7877_length_9490_cov_8.413249, whole genome shotgun sequence.
ACCESSION   DAOOYG010000097 DAOOYG010000000
VERSION     DAOOYG010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7877_length_9490_cov_8.413249"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PK288_08565"
     CDS             complement(<1..861)
                     /locus_tag="PK288_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM19017.1"
                     /translation="MTTRRMFLKKTAITTAGIGLAPAFSTVFARSAPSDRINIGLIGC
                     NGMGFANLKEFLNHSKVECLALCDVDENVLNQRAGDVEKIRGKKPVNLYHDWRRLIDN
                     KDINLVIVGTPDHWHCLQMVAACEAGKDVYCEKPIGRTIEECNLMVKAAKKYNSVVQV
                     GQWQRSDPHWQEAVDFIRSGKLGKIRLVRVFSYQGWCPSIPVLPDEPVPSGVDYDMWL
                     GPAPKRPFNRNRFHFTFRWFWDYAGGLMTDWGVHLLDYALYGMNVTAPRSIMAMGGKY
                     GYPDDACETPD"
     gene            1119..3812
                     /locus_tag="PK288_08570"
     CDS             1119..3812
                     /locus_tag="PK288_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPM19018.1"
                     /translation="MKKFLFVTWVLAVASCNPADRKTGTDYRRYDPFPLEVPEQNSLM
                     ASWEKKAVFETLVIDDMEHDAGWEVRGIGEMSYTEERAKDGSRSLRFMTSLRDEDYYR
                     KNRSKWDSFNGGQGGSSSLSLRFSKPQDWSGYNRLSFWVYVHPTSMPAYSFNININND
                     GFVPGATTSRRDHFIHDLKPGEWNHVMFEFPHLPRNKVTVFTINQILRGHNPEEEGIV
                     TYDFDKIEIQRVEADQYEGWNVAPGKFSFSHTGYRPGDNKTAFAGAGAGSDFQIIDPG
                     NNVVYNGSVRTIETMNGTFRLIDFSDLRTEGTYRIRSGSLESNPFPIGENIWLGPVFN
                     AINFFFCERCGFNVPGVHLECHKDWQGFRGDERKIINGGWHDAGDLSQGYWRTAMAVF
                     AMLNNLDALVQKKTDPELSDRIRNEAAWGLDWLLKTRFGDGYHMSWALGRIYTDNKIG
                     TIDDMVSEAQNVPWENFLAAAVLGRCAEAFKKFNPDLAERAFIAATEDWEAAIASRAA
                     WDLAEYREASWGVTSSVILNRITGEDKYKKQALQLAELLIKCQEQSFIEGIPITGYFY
                     TTSERRQVIHNYHAAFEEAPLIALSHLCNAYPDDGKWMDWYGAAVLHSEFFMKRGSAI
                     AAPYDLLPNSVWNRREIMAEKDEKLRADLLRQFNDGTHLSDEYVLRTFPIYFNDLFHG
                     NTNINLSSAWALAEASRLRKDKAGMEIAGKQLQWVLGANPFSQSLMYGTGYDFAPHFA
                     YCLKNLVGALPVGMDCMSGDEPFWSGTNTATYKEIWVEPVSRFLGAVSVFANYADPAS
                     NGDRGKVWFSTETQRSENGEIELTVSISGKGKHKIGIRTFNAVADKTEHEAVLSGNKP
                     VVLKVRLTDIDPEKPWVTVIMADNDPGLSSEVVGSFVEPTF"
     gene            complement(4021..5391)
                     /locus_tag="PK288_08575"
     CDS             complement(4021..5391)
                     /locus_tag="PK288_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPM19019.1"
                     /translation="MRTKYFYELQTIIGFFVIVLFSLSPLKVYSQDVKQTEERMMRQA
                     DENIEKYRKGDITIQFITPDGKPLRNATVEIRQKTHDFLFGCIIFDLIRGENPYKPEL
                     FKERFKKIFNLAVFPFYWPGYESRQGFTQWEAMLPVIDWCKLNGITTKGHPLVWATQS
                     GMPQWLSEYNVNETEELLKTRVLNITSGFRDKIELWDVVNEPIHVRTWKNKIRNFNDE
                     NDWDISESIPLVADYVEDALRWAKQGNPKATLLINEYRTLADESSRKSYELLLKELKK
                     RNAPLSGIGIQAHEPRQEWFSPIEVWKTFDLYWGMGFPIHITEYHPQSSGVPITGIWR
                     SGSWTPEAQVESTEQFVKLCFGHPGVVSINWWGFSDRNIWLPGGGLVDEEYRPKPVYL
                     MLDKLINQTWKTSISANTGNQGTVSFRGFFGEYDIVVSKPDGNIEVFPVHVSKKEENK
                     WVFTTH"
     gene            complement(5408..6583)
                     /locus_tag="PK288_08580"
     CDS             complement(5408..6583)
                     /locus_tag="PK288_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HPM19020.1"
                     /translation="MKKFLILFSLVVPFILYSCNGDQVKKEDDRLKVMAYYYTGWRNF
                     TPDILPYDKLTHIIFSFTEVIDGEMKFPEDSLGLILNELVKRKKTHNNLKIMIACGGW
                     AGSGGFSEMARTQQGRDRFVESVVKFIRQYKLDGLDIDWEYPGLPGIGNPYIPEDREN
                     FTALMCELRKGLNTISDSQVLTFASAGWERAFDFIELDKVMKCVNYINVMSYDLAGGG
                     DPYTSHHTNLGWVRMEDIKGTPAEAKIIERGESTKPFSTEKMVAFLMARGVNPAQIVI
                     GSNFSGRAWQGVPPENNGLYQLNRGAWRGRGSYARIREGMENMNGFVRYWDPVAKAPY
                     LYNAKDSIFITYDDTMSVRLKTRYVKDLGLGGIMFWQLGADAPQNGLVDAIYKEKMK"
     gene            complement(6663..8483)
                     /locus_tag="PK288_08585"
     CDS             complement(6663..8483)
                     /locus_tag="PK288_08585"
                     /inference="COORDINATES: protein motif:HMM:NF039640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6057 family protein"
                     /protein_id="HPM19021.1"
                     /translation="MKKRNPDKKQPVGKEDAKFLSNIGRTGIYILTVSVFILSLICFG
                     LFFNYVFFYQEKQSLFVYSYDYLSRFVSKPGGMLEYAGNFIAQGFFSNLYGAIVVSVF
                     LAAIALVYYRIAAVLTNRYLFPLLLAAIAACLLILIQTNINYQIHNSLGFLAVGLYFL
                     FAISQDGRNARISVFVFFPFFYYLTGAFALIFLGMFISYHLFRKEFRDTIIILLIAGV
                     SLVIFKRIISLQPWLNLFYDPLPGKDYFIRPWLLWLFLLFFVLYPALVSAAGNLKVKS
                     EFAALFPLVSSGIVILLTVFMLSRIYKHDNAVLFNIERMFFSADWNGVIRLQEKHRLR
                     SPVAQYYYNIALSEKGLLCDRLFFGPQDYGTGSISIPWNSQISMTKIFRGVYFYYSIG
                     LINEAHRWAFESMVVQGFHPENIKMLIKTNLINGHYKIAAKYIYVLKKTFHYRKLAKE
                     FEKMLSDPELIKSDPELGEKIRLKPNDDFIIRIRNPKMNIISLSESNPHNKKAFEYKM
                     AWRMLEKDVEDVVGDLSTLIGLNYSRIPRHIEEALLIYRMVFGQFADLNLVTISSDTE
                     ARFSRYRSVLGPMSGKKYPAGTAIPGELRNTYWYYLDFKK"
     gene            complement(8517..>9490)
                     /locus_tag="PK288_08590"
     CDS             complement(8517..>9490)
                     /locus_tag="PK288_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C biosynthesis protein"
                     /protein_id="HPM19022.1"
                     /translation="NRLLKTNCINCHSFSGNNPDRLMVHVRGSLGGTYFTEGKRIVRR
                     DLKADEMEYGAVYPAWHPGGTIVAFSSNSIVQSFHASQVYNIEVTDLASSLVVYDAEN
                     NEMSPVTETDSVPYLETFPEWSPDGRFLYYCRARQTADSYASDETKYDLVRKPFDMSS
                     FSFGKAEVVFDACAIGKSVSFPRVSPDGKYLVFTLHNSGNFSIWHKEADLYLLDIQTG
                     KAERMSVNSEFTESYHSWSSNGKWLVFSSKRDDGLTARPYIAWFASADSVGKPFALPQ
                     KDPALYGHLIKTFNRPEFVKGRIRFGPRDFERASGRKALKAGWRGRK"
BASE COUNT         2568 a         2198 c         2105 g         2619 t
ORIGIN      
        1 gtcgggcgtt tcacaggcat catcgggata gccgtatttc ccacccattg ccattattga
       61 tcggggagca gttacattca ttccgtacag ggcataatcg aggagatgta ctccccagtc
      121 agtcataagc ccgccggcat aatcccagaa ccaccggaag gtaaaatgga atctgttccg
      181 gttaaacgga cgcttgggag cagggcccag ccacatatca taatcgacgc ctgaaggaac
      241 aggttcatca ggtaaaaccg gaattgaggg gcaccatccc tggtatgaga atacccgtac
      301 aagcctgatc ttccctagtt tgcctgaccg gataaaatcc actgcttcct gccagtgggg
      361 atcgctcctt tgccactgtc cgacctgtac aacactgttg tatttttttg ccgcctttac
      421 cataagattg cactcctcaa tggtccgtcc gatcggcttt tcacaataaa cgtccttgcc
      481 ggcctcacag gctgcaacca tctgaaggca atgccagtga tcgggggtac ccacaatcac
      541 gagatttata tccttgttgt cgataagcct tctccagtcg tggtaaagat tgaccggttt
      601 ctttccgcgt attttttcaa catctccggc tctctggtta agtacatttt catcaacatc
      661 acaaagggca aggcactcaa ccttgctgtg attgagaaac tccttcagat tagcgaatcc
      721 catcccgttg catccgatga ggccgatatt gatcctgtcg gaaggagcgg accgtgcaaa
      781 tacggtggaa aaagccgggg cgaggccaat gccagctgtg gtgatggcgg tttttttcaa
      841 aaacatgcgt cgtgttgtca tatttttata gtttgaggtt ttgataaagg gcacagaatt
      901 taatgattca atatacctaa attttttcaa atggtatttt taccggatca atttaaacaa
      961 aatgagaaat atttatcact ggcctggggg tatgataaat tcctggtttt tacatcttcg
     1021 gggtgaaaga tgacagcccg ggcagaatat aggccggtta tgatgaaaat ttcttaactt
     1081 tattggtaat cttattatta ccgtaaatcc ttcactgtat gaaaaaattt ctatttgtaa
     1141 cctgggtcct tgctgttgct tcatgcaatc ctgcagacag aaagaccgga acagattatc
     1201 gtcgctacga tccgttcccc ctcgaggttc ctgagcaaaa ctccctgatg gcaagctggg
     1261 agaagaaggc tgtttttgaa accctggtca ttgatgatat ggaacacgat gccggctggg
     1321 aagtaagagg tatcggggag atgagctaca ccgaagagcg tgcaaaagac ggaagccgtt
     1381 cactcaggtt catgacttca ctacgtgatg aagactatta caggaaaaac cgcagtaaat
     1441 gggattcatt caacggagga cagggaggat cttcatccct cagtctccgg ttcagcaaac
     1501 cgcaggactg gtcgggctat aacaggcttt cattctgggt ttacgttcat cccacttcca
     1561 tgcctgcata cagttttaat atcaacataa acaacgacgg atttgttccg ggtgcgacaa
     1621 cttcgagacg cgatcatttc atccacgatc ttaagccagg tgaatggaac catgtgatgt
     1681 tcgagtttcc gcacctgccc cgcaacaaag tgacggtgtt caccattaat cagattttga
     1741 ggggacacaa tcctgaggaa gaaggtattg tgacgtatga tttcgataaa atcgagatac
     1801 aaagggttga agcagatcag tatgagggat ggaacgttgc tccgggcaaa ttttcgttca
     1861 gccatacggg ctacaggccc ggggataaca agacagcttt tgcaggtgcc ggcgccggga
     1921 gcgacttcca gatcatagat cccggcaata atgtggttta taatggtagt gtcagaacca
     1981 ttgaaactat gaacggcact ttcaggctga ttgatttttc agatctcaga acggaaggca
     2041 cataccgtat caggagcggt tcccttgaat ctaatccgtt tccaataggt gagaacatct
     2101 ggctcggacc cgttttcaat gcaatcaact tctttttttg tgaacgctgc ggtttcaatg
     2161 ttccgggagt tcatctggaa tgtcacaagg actggcaggg attcagggga gatgaaagga
     2221 agatcataaa cggtggctgg catgatgcag gcgatctttc gcagggttac tggcggaccg
     2281 ccatggctgt ttttgcgatg ctgaacaatc tggatgcatt ggttcagaaa aaaacagatc
     2341 ccgaactctc ggatcgtatc aggaatgagg cagcatgggg tctggactgg cttctgaaaa
     2401 ccaggttcgg ggatggttac catatgagct gggcacttgg caggatctat accgataata
     2461 agatcggtac aattgacgac atggtatcgg aggctcagaa tgtgccgtgg gagaatttcc
     2521 tggcagcagc tgtactgggc agatgtgcag aagcatttaa aaagttcaat ccggatcttg
     2581 ccgaaagagc cttcattgca gcgactgagg actgggaagc cgcaattgct tcgcgtgctg
     2641 cctgggatct ggccgagtac agggaagctt catggggtgt aacatcttca gtgatactga
     2701 acagaataac cggagaagac aaatataaaa agcaggcttt gcagcttgca gaattactta
     2761 ttaaatgcca ggaacaaagc tttatagagg gcattccgat aaccggttat ttttacacca
     2821 catcagagcg ccggcaggtt atacataatt accacgcagc cttcgaggag gctccattga
     2881 tcgcattatc gcatctctgc aatgcttatc ctgacgatgg aaaatggatg gactggtatg
     2941 gtgcagccgt tctccacagt gaattcttca tgaaaagagg aagcgcaatc gcagctcctt
     3001 acgatctgct tccgaattca gtatggaacc gccgtgaaat catggctgaa aaggatgaga
     3061 aactccgggc ggacctcctg cgtcagttca atgatggcac acatctgtct gatgaatatg
     3121 ttctccggac tttcccgata tatttcaatg atcttttcca cgggaacacc aacataaacc
     3181 tttcttccgc ctgggcgttg gctgaagcat cgcgcctgag aaaagacaaa gcgggaatgg
     3241 aaattgccgg aaaacagctt caatgggtct tgggagccaa tcccttcagc caaagcctga
     3301 tgtatggaac aggctatgat tttgcaccgc atttcgctta ctgcctgaaa aatcttgtcg
     3361 gcgccctgcc cgtgggaatg gactgcatgt cgggtgacga acctttctgg tcgggaacaa
     3421 atactgccac ctacaaggag atatgggtgg agcctgtaag caggttcctg ggggccgttt
     3481 cggtattcgc aaattatgct gatccggctt caaacggtga taggggtaaa gtatggttca
     3541 gtacggaaac tcaacggtca gaaaatggag aaatagagct gacagtttca atttccggaa
     3601 agggtaaaca taagatcggg atcaggacat tcaatgcagt tgctgacaag acagaacatg
     3661 aggcagtcct gtcaggcaac aaaccggtcg ttctcaaagt caggctgact gacattgatc
     3721 ctgaaaaacc ctgggtaaca gttatcatgg ccgacaatga tcctggcctg agcagtgaag
     3781 tcgtcggatc attcgttgaa ccaacttttt gattaaaagt ggcactgaac aaagatccct
     3841 tccttgctgg cgccgatctg acttcgtcga gctcgcaaaa acaattattt taggtcactt
     3901 cgtgaattaa ccattttgcg ttttgcccgg ttgtaatctg tccccattat ataggtaaga
     3961 agtggcgcgg atctgcgatc cgtgccagtg gggatctgcg ccagctggct atcttcagta
     4021 tcaatgcgta gtgaacaccc acttgttttc ctcttttttg ctcacatgaa caggaaatac
     4081 ctcaatattg ccatccggct tactgactac aatatcatat tcaccgaaga atcccctgaa
     4141 tgaaaccgtt ccctggttgc cggtatttgc cgatatgctg gttttccagg tctgatttat
     4201 gagtttgtcg agcataagat agaccggttt gggtctgtat tcctcatcga caagacctcc
     4261 gcccggaagc cagatgttac ggtcggagaa accccaccag ttgatggaaa ctacccccgg
     4321 atgtccgaaa cacagtttta caaactgttc tgtagattca acctgggctt ccggcgtcca
     4381 tgatccgctc ctccatatcc ccgttattgg tacccctgat gactgaggat gatattcggt
     4441 tatatgtatc ggaaatccca tcccccagta gaggtcgaaa gttttccaga cctctatggg
     4501 agaaaaccat tcctgacggg gttcatgagc ctgtatccct atgcctgaaa gaggagcatt
     4561 tctctttttc agttctttca acagcaactc atagctcttc ctggaacttt catcagccag
     4621 tgtcctgtat tcgttgataa gcagtgttgc tttgggattt ccctgctttg cccatctcag
     4681 tgcatcctcc acataatcag cgaccaatgg gatggattct gaaatatccc agtcattctc
     4741 atcattgaaa ttccggatct tgtttttcca ggtcctgaca tggatcggtt cgtttaccac
     4801 atcccagagt tcgattttgt cacggaatcc tgatgtaata tttaaaaccc ttgtcttcag
     4861 gagttcttca gtttcattta cgttatattc actcagccat tggggcattc ccgattgtgt
     4921 agcccaaacc agcggatggc cttttgtcgt tatgccattg agtttgcacc agtcgattac
     4981 cggtaacatt gcctcccact gagtaaatcc ctgtcttgat tcgtatccgg gccagtaaaa
     5041 tggaaatact gccaggttaa agatcttttt gaacctctcc ttaaaaagtt ctggtttata
     5101 tggattttca cccctgatca ggtcgaaaat tatacatcca aagagaaaat cgtgtgtttt
     5161 ctgcctgatc tccactgtgg cattccgcag aggtttcccg tcgggagtaa tgaactggat
     5221 agtgatatcg cccttccggt atttctcaat attttcatct gcctgcctca tcatcctttc
     5281 ttcagtctgc tttacatcct gtgaatatac ctttaaaggt gacagtgaaa aaaggacaat
     5341 gacaaagaaa ccaattatgg tttgcaattc ataaaaatac ttggttctca tgtttattaa
     5401 ttttttatca tttcattttt tccttgtata tggcatccac caggccattc tgaggagcat
     5461 ctgcgccaag ctgccagaac attatacctc ccaggccaag atccttgaca taccgggttt
     5521 taaggcgtac cgacatcgta tcatcatatg ttatgaatat actgtctttg gcattataca
     5581 ggtatggtgc ttttgcaacc ggatcccagt agcggacaaa tccgttcata ttctccattc
     5641 cctccctgat ccttgcataa ctgccgcggc ctctccatgc tcccctgtta agctggtaca
     5701 atccgttgtt ttccgggggg actccctgcc aggctctgcc tgagaaattt gatcctatca
     5761 ctatttgagc cgggtttaca cctcttgcca tcaggaaagc aaccattttt tcggtcgaga
     5821 agggttttgt tgattctcct ctttcaatta ttttggcctc tgccggcgta cctttgatgt
     5881 cttccattct gacccatcca agatttgtat gatgtgaggt atagggatct cccccgcctg
     5941 caagatcgta ggacatgaca ttgatgtagt ttacgcactt cataacctta tcaagttcaa
     6001 taaaatcaaa ggccctttcc cagccggctg aagcaaaagt aagaacctgg ctgtcactga
     6061 tcgtgttaag gcctttgcgc agttcacaca tgagtgcagt aaaattctcc ctgtcttcag
     6121 ggatgtaagg attgcctata cctggaaggc cgggatattc ccagtcgata tcaagcccgt
     6181 caagcttgta ttgcctgata aatttcacaa cactttcaac aaacctgtcg cgaccctgct
     6241 gcgtcctggc catttctgag aacccccccg aacctgccca gcctccgcag gcgatcatga
     6301 ttttcaggtt attatgcgtt ttcttccgtt taaccagttc attgagaata agtcccaatg
     6361 agtcttccgg gaatttcatt tctccgtcaa ttacttcggt gaatgaaaag atgatatgag
     6421 tcagcttgtc atacggaaga atgtcaggtg taaaatttct ccagccggta taataatagg
     6481 ccatcacttt aagcctgtca tcttccttct ttacctgatc accgttgcag gaataaagta
     6541 taaagggaac gacaagggaa aaaaggatca gaaacttttt catagcttag gatttaaaag
     6601 attattttat atgatctgaa tctgttttca gttccgggca atcaatatta ctgaagttaa
     6661 attcactttt taaaatcgag gtagtaccag tacgtgtttc tcagttcccc ggggatcgcc
     6721 gttcctgcag ggtacttttt acctgacatc ggacccagta cagatctgta tcgtgaaaac
     6781 cttgcttcag tatcgctgct tatggtaacc agattcagat ccgcaaactg gccaaaaacc
     6841 attctgtaaa taagtaatgc ctcttctatg tgccttggaa ttcttgaata gttcaaccct
     6901 atcagggtgc tgaggtcgcc gacaacatcc tcaacgtcct tctcaagcat tctccacgcc
     6961 attttatatt caaacgcttt tttattatgc ggattagact ctgaaaggga aattatattc
     7021 attttgggat tcctgatcct gattataaaa tcgtcgtttg gtttcagtct gatcttctct
     7081 cccagttcgg gatccgattt tatcagttcg ggatccgaaa gcattttttc aaattccttt
     7141 gccagtttcc tgtaatgaaa tgtttttttc aggacatata tgtatttggc cgctatcttg
     7201 taatgtccgt tgatcagatt tgttttgata agcattttaa tattctccgg atgaaaaccc
     7261 tggacgacca ttgattcaaa agcccaacgg tgtgcctcat tgatcaggcc tatggaataa
     7321 tagaaataga cgcccctgaa gatcttggtc atgcttatct gagagttcca gggtatactg
     7381 attgaccccg ttccgtaatc ctggggaccg aaaaacagtc tgtcacagag caaccccttt
     7441 tctgataatg caatattata ataatattgt gcaaccggac tcctgagcct atgcttctcc
     7501 tgcaacctga tcacgccgtt ccagtctgcc gaaaaaaaca ttctttcaat gttaaagagc
     7561 acagcattgt cgtgtttgta aatccttgaa agcatgaata ctgttaataa aataacaata
     7621 ccggaagaaa caagcggaaa taaagcggca aactcacttt tgaccttaag gtttcctgca
     7681 gcggacacta gcgcgggata taaaacaaaa aaaaggagga acagccacaa aagccatggc
     7741 cggataaaat aatccttccc ggggagagga tcataaaaca ggtttaacca tggctgcagg
     7801 gaaataattc ttttgaagat cacaagtgaa actcctgcaa ttaacagaat tattatcgta
     7861 tcccgaaatt cttttctgaa aagatgatag ctgatgaaca tgccgagaaa aatcagggca
     7921 aaagccccgg tcagataata aaagaatggg aaaaaaacaa aaactgaaat cctggcattc
     7981 cttccgtcct gagatattgc aaataaaaaa tacaatccga ccgccaggaa ccccaaacta
     8041 ttgtggatct gataattaat atttgtctgg atcaatatca gcaggcaggc ggcaattgca
     8101 gccagtaaca acgggaacag gtacctgtta gtcagtacgg cagctatcct ataatataca
     8161 agagcaatcg ccgccaggaa cactgataca acaatggcac cgtaaagatt actgaaaaat
     8221 ccctgggcaa tgaaattccc tgcatattca agcattccgc caggttttga gacaaaccgg
     8281 ctgagataat cataactata aacaaaaagg gattgttttt cctgataaaa gaaaacataa
     8341 ttgaaaaaca gtccgaaaca aattaatgag agaataaaaa cagaaactgt caatatataa
     8401 ataccggtcc tcccgatatt ggaaagaaat tttgcatctt cctttccaac tggttgtttt
     8461 ttatccggat ttcgcttttt cattcggcag atatgatgat atcataaact ggaatattat
     8521 tttctccctc tccatccggc ttttaaagct ttccttcctg aggcacgttc aaaatcgcgc
     8581 ggaccgaatc ggattcttcc tttcacaaat tcaggcctgt tgaaggtttt tatcaggtgg
     8641 ccgtagaggg caggatcttt ttggggaaga gcaaatggtt ttccgacgga gtcggccgat
     8701 gcaaaccagg caatgtaagg tcgggcggtt aatccgtcat ctcttttact gctgaacacc
     8761 agccattttc cgtttgacga ccagctgtga taactttcag taaactcact gttcacactc
     8821 atccgctcag ctttgccggt ctgaatatcc agcaggtaca ggtcagcttc cttgtgccag
     8881 atcgaaaaat tcccgctgtt gtgaagtgta aatacgagat atttcccgtc gggagacacc
     8941 cttggaaaag agacgctttt gcctatggca caggcatcga agacgacttc tgcttttccg
     9001 aaggaaaatg aactcatgtc aaacggtttc ctcacaagat catacttggt ttcatcggaa
     9061 gcgtaactgt cagcagtctg cctggccctg caataataca ggaaacggcc gtccggcgac
     9121 cactccggaa atgtctccag gtaaggaacg gagtctgtct ccgtaacggg tgacatctca
     9181 ttattttctg catcataaac cactagtgag gatgccaggt cggtaacctc aatattgtac
     9241 acctgcgacg catgaaaact ctggacaatg ctgttggatg aaaaggccac aatggttcct
     9301 cccggatgcc atgcaggata tacggcacca tactccattt catccgcctt cagatctctc
     9361 ctcacaatcc ttttcccttc cgtgaaatat gtgccaccga gagaacccct gacatgaacc
     9421 ataagcctgt cgggattatt accgctgaat gaatgacaat taatacaatt tgtcttaagc
     9481 agccggttgt
//
LOCUS       DAOOYG010000098         9427 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_7953_length_9427_cov_9.037665, whole genome shotgun sequence.
ACCESSION   DAOOYG010000098 DAOOYG010000000
VERSION     DAOOYG010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7953_length_9427_cov_9.037665"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PK288_08595"
     CDS             <1..191
                     /locus_tag="PK288_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19023.1"
                     /translation="ALPPDLRIFDPQKALSPLDEDFDWESLKKSPILDDPREMVEDQK
                     EKPEYRLEDQKSDPVIRP"
     gene            681..995
                     /locus_tag="PK288_08600"
     CDS             681..995
                     /locus_tag="PK288_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19024.1"
                     /translation="METINRKSSGKAAKGSVVNGSSAGRVPLFRAKYLLTFILPVFLL
                     MCIMPAEASAGTGKGRNSSCHARKVSFAYPVVASKKNNPVINVSKKHSKKSGSRNHSF
                     PI"
     gene            complement(1078..1572)
                     /locus_tag="PK288_08605"
     CDS             complement(1078..1572)
                     /locus_tag="PK288_08605"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPM19025.1"
                     /translation="MKRREILKNLSVVPLAGVIGNDITGPEKNKSKEEWTPVKEAIPG
                     VYPTTSPLSLCIRSGHLVFLSGIGGWYANRRAEPGDIKVQIESALTDMKNQLEKAGTS
                     MANVLKVHMTLADPNKNLAPLNEVYGKFFPDPKPARSFSGAGIDQMGRDGVLVQIDCI
                     AYID"
     assembly_gap    1821..1920
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2164..2236)
                     /locus_tag="PK288_08610"
     tRNA            complement(2164..2236)
                     /locus_tag="PK288_08610"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Gln,seq:ctg)
     gene            complement(2344..4044)
                     /locus_tag="PK288_08615"
     CDS             complement(2344..4044)
                     /locus_tag="PK288_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPM19026.1"
                     /translation="MSEEDIITGEESRSPVSFIEQIIIDDLGEGKNGGRVHTRFPPEP
                     NGYLHIGHAKAICLDFGMAEKYGGKCNLRFDDTNPVKEEVEYIESIKEDIRWLGFDWE
                     DREYYASDYFGKLFDFAIDLIKRGLAYVDDQPAEVISAQKGTPAQSGTESPYRNRPVG
                     ENLDLFERMNRGDFEEGSRVLRARIDMTSPNMHMRDPIIYRIIKSSHHRTGEKWKVYP
                     MYDFAHGQSDYFEGITHSLCTLEFEVHRPLYDWLIDQLKTGEYRPRQIEFNRLNLTYT
                     VMSKRKLLELVQDGKVSGWDDPRMPTLCGLRRRGYTPESIRKFIDLIGYTKYEAINDI
                     SLLEHAVRDDLNLRAPRVFGVLNPLKVVITNYPENMVEEVDLINNPEDETMGSRKVPF
                     SRDLYIEQDDFMEDPPHKFFRLSPGSEVRLKGAYIIKCTGYTKDENGRIIEVLCTYDP
                     HTRSGGHLSGKKVKGTLHWVSAAHALDAEVRLYDRLFDHEDPSGQKEDDYRKFLNPDS
                     LRILRGCKVEPSLKTAMHLDKFQFQRLGYFCIDYDSTPDHLVFNRTVGLKDSWAKMNK
                     "
     gene            complement(4613..4903)
                     /locus_tag="PK288_08620"
     CDS             complement(4613..4903)
                     /locus_tag="PK288_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPM19027.1"
                     /translation="MRTFRELDPVIHSQFRLAVISLLIGVEVAEFTWLREQTEATAGN
                     LSVQISKLKEAGYIDVVKKFSNNYQQILCRITPPGRNKFAEYGEALKDYLNP"
     gene            complement(4890..5252)
                     /locus_tag="PK288_08625"
     CDS             complement(4890..5252)
                     /locus_tag="PK288_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19028.1"
                     /translation="MIYGYIKGKSSPFHTYAETLYRWTWMGFMIAAIVPFIIISDRIE
                     SVAPFIITLAGIPTFISVFIIKFRPLIFGGIALRISALIARFWGNEISAFAVSLALFT
                     GYLLPGYMLKRKVDHENV"
     gene            complement(5288..5419)
                     /locus_tag="PK288_08630"
     CDS             complement(5288..5419)
                     /locus_tag="PK288_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19029.1"
                     /translation="MINRTKVNIRQGSFHLLFWGWLIFFCSLGEYLLVRLTAFAVHD"
     gene            5621..5971
                     /gene="folB"
                     /locus_tag="PK288_08635"
     CDS             5621..5971
                     /gene="folB"
                     /locus_tag="PK288_08635"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPM19030.1"
                     /translation="MGLIRIEGMEFYAFHGHYQEEQKVGNRFLVDLTIETDMTKPSES
                     DNLKDAVNYQAAYGIVKKQMEKKSYLLENIAGRILSAIHAELDGVKKVVVKVSKLNPP
                     IGGKISSVSVVLEG"
     gene            6038..9319
                     /locus_tag="PK288_08640"
     CDS             6038..9319
                     /locus_tag="PK288_08640"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPM19031.1"
                     /translation="MRLLVVSLSVILVTINCAGPHPENHDPVTVQMLEDGFVSPPDSV
                     KPWVYWYWISDNNTREGITKDLEAMADAGIGEAMIGNIGYEWMPYGDSRILGEEWWQL
                     IEHAIREGKRTGVDIGIFNSPGWSQSGGPWVGPGESMRYLYCTEMTVEGGTRVRRKLS
                     NPVENYQLLKVLGFPSPESEYSVLNSANAVITTIPRLDNTINLIDGDTGTICLFRRAG
                     KASEILIELESREPFVARSLKLFPAGISFAADVEVMALAEDGSYRSVRTFRYDRSNPR
                     INVGPVPEAPVAVSLPETSSTRFRMILNNFSLSNYLTSRPDNASDAGLSEIVLSGVPV
                     VESYVEKQLGKMCQTPFPLWNEYQWIPQQESGSGEMKINPMEVVDLTSGMSADGTLDW
                     DVPGGKWIILQIGAVPTGAMNAQAAPNAVGWEVDKMNRDHLKRHFDAYIGNLIRRMSP
                     DERTALKHVVLDSYEQGSQNWTEGMIGDFMARYEYNPVPWFPVLTGRVVGSADQSDRF
                     LWDWRRLVADRIAYDYVGGFRELCAANGLRIWLENYGHWGFPSEFLMYGGQSHDVAGE
                     FWNEGELGNIECRAASSAAHIYGKRLVSAESYTSAGQAYQRYPAMLKKRGDWSYTEGI
                     NQVVLHLYIHQPYEDKNPGINAWFGTEFNRKNTWFRQSKHWIDYQRRCMFMLRRGLPV
                     NDVCYFIGEDVPKMTGVRIPELPPGYSFDYINAEVILDRLTVKDGLLVLPDGMSYRLM
                     VLPPLETMRPEVLKKIGMLVEKGAKIYGPPPSRSPSLQNYPEADRTVQRLTGELWQTE
                     KADKPSGGYVMDNMSLEEALNSAGVQPDVSLPESTPVLWIHRRMKDCDIYFITNQSDE
                     RISFDAGFRVGKMQPELWDPLNGMIRDLGEFSHTSGMTVTPLQFEPCGSAFIVFRKGS
                     GDRSEKPVGPTNFPVPVTLVEINTPWEVTFERKPNGPEKSIMMEIPEDWSKSLDERIR
                     YYSGSAIYRNSFTLGPIPAGENVIIDLGKVGVMAEVKVNGRFAGGTWTQPWSVDITEM
                     VKQGENTLEIEVVNNWINRLIGDSRLPEKERSTWLNENSVRPDDPLQASGLIGPVVIS
                     SVKYR"
BASE COUNT         2571 a         1968 c         2316 g         2472 t
ORIGIN      
        1 aagcgttacc tcccgacttg cggatatttg atccgcagaa agcactttca ccactcgatg
       61 aggattttga ctgggaatcg ctgaaaaaat caccgatcct tgatgatccc agggagatgg
      121 ttgaggatca gaaggagaaa cccgaatacc ggctggagga tcagaaaagt gatccggtaa
      181 tcaggcccta gtatcaggtc agcatcagtc tgaaatgccg gctgtgcttt ctgccggttg
      241 cagcactgct gatttggcac agggttatgt ttccgcccgt ttgccctccg gtcatttcct
      301 ttgctgacag tcgggcttcc aggtaaaagg cggctgactc gtggaaaaat ttatgacaac
      361 tgttcaggca ggttgtcagg atgagcatgg aaaactaaat catggagttt tttctttgtc
      421 agattccatt ggtcaacaga ttcaggatgt tcgtcaaatc cgcagatatc tgcctgcatc
      481 taaccttgtg ttttgtaatt aacaggaata cagtacgcta cataaacagg aaattcattg
      541 aggacaccta tgccggtttg tatctgtcaa tcggcgtgag taattccttt acaatcttcg
      601 ttaaattggt tgagaactta acccggatac agtgataagc tgaaaggaaa atgagaaccc
      661 aaatgaaaca ctaaggaatc atggaaacaa tcaacaggaa atcgtcaggg aaagcagcaa
      721 agggatctgt cgtgaatgga agctcagctg gaagagtacc gctttttagg gcaaaatacc
      781 tgctcacatt cattcttccg gtgtttcttt tgatgtgcat tatgccggct gaggcatcag
      841 caggtacggg aaaaggcaga aattcttcat gtcatgccag gaaagttagt ttcgcttatc
      901 cggttgtagc ttcgaaaaag aataatccgg ttattaatgt ttccaaaaag cattcaaaaa
      961 aatccggaag cagaaatcac agttttccga tatagattca aaaaggttcg gctgcaaaat
     1021 atttaagcct tatatcaggt attttctaca tcggaagaga tttcagtaaa atatttctca
     1081 atctatgtat gcaatacagt cgatctggac cagaacgccg tcacgtccca tctggtcaat
     1141 tcctgctccg ctgaaggagc gtgcaggttt cgggtcgggg aaaaatttgc catagacttc
     1201 attgagaggt gccagattct tatttggatc agcgagtgtc atgtgtactt ttaaaacatt
     1261 ggccattgaa gtgcctgctt tttccagttg attcttcatg tctgtaaggg cgctctcaat
     1321 ctgcactttt atgtcccccg gttcggcacg cctgttggca taccagccac ctatgccgct
     1381 taaaaaaaca agatgacccg aacgaatgca taatgacagg ggactggttg taggatagac
     1441 gccgggaata gcctctttaa cgggagtcca ttcttccttt gatttatttt tctcaggtcc
     1501 tgttatgtcg ttgcctatca ccccggcgag cggaaccacg gaaagatttt taagaatttc
     1561 tcttcttttc atttaaaatt gatatttcag ttgaaattac gaaaaataag ctatcctggt
     1621 cttaactgtt ttataaattt cataaatttt aattaaatgc aaggataatt ctgttaccag
     1681 tcgttttatt ttttatttca actgttttca gaaatttgat aataattaga caaaaaagct
     1741 gcaaatgctt gcagcttttt gttgctccgt cagggctcga accgggccga gcgttaagtt
     1801 ccgacatgtt atgtcggaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 ctgccgcggt ggcgttcggg ccctcttatg ttcatggttt tttggataaa aaaaaagcca
     1981 acaaatcatt gtcggtttat ttaagttgat ccgtcagggc tcgaaccggg ccgagcgtta
     2041 agttccgaca tgttatgtcg gaatagcgaa ggagccagac tgccgcggtg gcgttcgggc
     2101 cctcttatgt tcatggtttt ttggataaaa aaaagccaac aaatcattgt cggcttattt
     2161 aagttgctcc gtcagggctc gaacctgaac ttttctgatc cagagtcaga cgtgttgcca
     2221 gttacaccac ggagcagtat tcgggggcaa agataaatat aaatgtaaaa accacaaaca
     2281 ggaataacag ctaacctgtt gggatctgac aggtgaatag tagtctcatc cgttatccgg
     2341 cggctatttg ttcatcttcg cccatgaatc tttcagtccg acagttcggt tgaaaaccag
     2401 atgatcgggt gttgaatcat agtcgatgca gaaataaccc aggcgctgga actggaactt
     2461 gtcaagatgc atggctgttt tcagcgacgg ctcgactttg cagcccctga ggatcctgag
     2521 tgaatcggga ttgaggaact tccggtaatc gtcttctttc tgtcctgacg gatcctcatg
     2581 atcaaaaagg cggtcgtaca atctcacctc tgcatccagg gcgtgagctg cggaaaccca
     2641 gtggagcgta ccctttacct ttttgcctga taaatgaccg ccgcttcttg tatgagggtc
     2701 ataagtgcaa agaacttcaa tgatcctgcc attttcatct ttagtgtagc ccgtgcattt
     2761 gattatataa gctcccttga gtcgtacttc gctcccggga gaaagcctga agaacttgtg
     2821 cggcggatcc tccatgaaat catcctgttc gatgtacaga tcacgggaaa aaggcacttt
     2881 acggcttccc atggtttcat cttcaggatt atttatcaga tcaacctcct caaccatgtt
     2941 ttcgggataa ttggtgataa ccaccttcag gggattcaat acaccaaaaa cccgcggagc
     3001 cctgaggtta agatcgtccc ggaccgcatg ctcaagcagg gaaatgtcat ttattgcctc
     3061 atattttgtg tagccgataa ggtcgatgaa cttccttatt gattccgggg tgtatcccct
     3121 cctccgcagg ccgcagagcg tcggcatcct tggatcatcc catcctgata cctttccatc
     3181 ctgaacaagc tcaagaagct ttcgcttgct catcactgtg tacgtgagat taagcctgtt
     3241 gaactcgatc tgtctcggac ggtattcgcc tgtcttaagc tgatctatga gccagtcata
     3301 caagggtcgg tgtacttcaa actcgagggt gcagagggaa tgggttatac cttcaaaata
     3361 atcactctgg ccgtgggcaa aatcatacat gggatacact ttccattttt ccccggtacg
     3421 atgatgggat gattttataa tcctgtatat tatcggatca cgcatatgca tgttcggaga
     3481 agtcatatca atcctggctc tcaggaccct gcttccttct tcaaaatcac ccctgttcat
     3541 cctttcgaac aggtcgagat tttccccgac gggccggttc ctgtaaggac tttcggtccc
     3601 ggattgggcg ggtgtgcctt tctgggctga aataacttcg gcgggctgat catccacgta
     3661 agcaaggccg cgctttatta gatcaattgc aaaatcaaag agctttccga aatagtcgga
     3721 agcatagtat tccctgtctt cccagtcaaa gccaagccat cttatgtctt ctttaataga
     3781 ttcaatatat tcaacttctt ctttaacagg attggtatca tcgaaacgca gattgcattt
     3841 tccaccgtat ttttctgcca ttccgaaatc aagacatatg gctttggcat gaccaatgtg
     3901 aagatagccg ttgggttccg ggggaaaacg ggtatggacc cttccgccat tctttccttc
     3961 cccgagatca tcaataatta tctgctcaat aaaactgacc ggtgatcttg attcttctcc
     4021 ggtgataatg tcttcttcac tcatctctca gaaatttgaa actacaaaaa taggatgaat
     4081 ttaagactca actatagaaa atgaattttt ccttgatgat acatttgcct caccccctct
     4141 tatcccccgc tcgcaggata gaaaaggagc ggaaaccatg cagtcatcag cccatctctt
     4201 ttaggagaag ggttggggtg aggtaaacca gtgcaggggt aggggcttag tgagaggtaa
     4261 ataatcaggg agtgaatttt gttgagtata attcacctca tcccccgtcc ccttctccca
     4321 ggagagaagg ggagcaggaa ccatgctgtc atcagccgct ctctttcagg agagaggtta
     4381 gggtgaggta aaccagtgca gggagacggg ctggggtgag gtaaataatc cgggaatgga
     4441 gttttgttga gtttaattca cctcatcccc cagccccttc tcccagggga gaaggggagc
     4501 aggaatcatg taatcatcag cccctctctt ttaggagaag ggttggggtg aggtaaaaca
     4561 atccggggga gcaagaatca gtactcattg cttataattt ttttattagt tttcagggat
     4621 tcagataatc cttaagcgct tccccgtatt cggcaaactt atttcttcca ggcggagtga
     4681 tcctgcacag gatctgctga taattgttgc tgaatttctt aaccacatct atgtatccgg
     4741 cttctttcag cttcgatatc tgaacgctca gattacctgc cgttgcctca gtttgctccc
     4801 tgagccaggt aaattcagca acctcaacac ctatcagaag tgagatcact gccagcctga
     4861 actgggaatg aatgaccgga tcaagttctc taaacgttct catggtcaac cttccttttt
     4921 aacatatacc cgggcaaaag ataccctgta aacaacgcca gcgaaaccgc aaaggccgag
     4981 atttcgtttc cccagaaacg tgcaatcagt gcagatatcc gcaaagctat tcccccgaat
     5041 atcaggggtc tgaatttgat gatgaagacg gaaataaaag tagggatccc tgcaagggta
     5101 attataaaag gtgcaaccga ttcaatgcgg tcagagatga ttatgaaagg tacaattgca
     5161 gctatcatga accccatcca tgtccaccta taaagcgttt ctgcataggt gtgaaaagga
     5221 ctgctttttc ccttgatata gccgtaaatc aaggatacaa atacacccgg ggctacaagg
     5281 agccagatca gtcatggact gcgaaggcag ttaacctaac aagaaggtat tcaccaaggc
     5341 tgcagaagaa tatcagccat ccccagaaaa gcaggtgaaa gcttccctgc cttatgttga
     5401 cttttgtcct gtttatcatt taagaaatga tctgcagact ttcgtctcct gtcaatactt
     5461 cttcgttggt ttccataatt atttttaatt ttaattgcgg caataagtag tcataaataa
     5521 attatacaat taaaatagtt tataatataa acctgatatt tgaacagaaa attaaatctg
     5581 ttttcagttt tttcttagta cagattataa atattttaac atgggtctta tacggattga
     5641 aggaatggaa ttttatgcct ttcacgggca ttatcaggaa gaacagaaag tcgggaacag
     5701 gttcctggtg gatctcacaa tagagacaga tatgacaaag ccatcggaat cagataattt
     5761 gaaggatgcc gtgaattacc aggcggcata cgggattgta aagaaacaga tggagaaaaa
     5821 atcatatctg ttggagaata ttgcaggaag gattttaagt gcaattcatg ccgaactgga
     5881 tggagtgaag aaagtagttg tcaaggtgtc aaaattgaac cctccgatag gtggcaaaat
     5941 aagttcggtc agtgtggtac ttgagggata gcaggattaa ttcgcagcag gggaaatggt
     6001 tatgacataa aaacaattcc atattaagat atttgtaatg agattgttag tagtttcgct
     6061 cagtgttatt ttggtaacaa taaattgtgc cggaccgcat cctgaaaatc atgatccggt
     6121 tacagttcag atgttggagg atggatttgt aagtcctccc gattccgtaa aaccgtgggt
     6181 ttactggtac tggataagtg acaataatac aagggaaggg atcacgaaag acctggaggc
     6241 aatggctgat gccggtatcg gggaagccat gatcggcaat atcgggtatg aatggatgcc
     6301 ttacggtgat agccgtattc tgggtgaaga atggtggcag ctgattgagc atgccataag
     6361 ggaaggaaaa aggacaggag tagatatagg gatcttcaac agtccgggct ggagccagtc
     6421 gggcggcccc tgggttggtc ccggggaatc gatgcgatat ctttactgta ctgaaatgac
     6481 agttgaaggc ggcacgaggg tgcggagaaa gctgtctaat ccagttgaga attatcagtt
     6541 gctgaaggtc ctgggatttc cctcacccga atctgagtat tccgttctca acagtgcaaa
     6601 tgccgttatt accactattc cccggctgga taacacgatt aatcttattg atggggatac
     6661 ggggacgata tgccttttca gaagggcagg aaaggcgtcg gaaattctga tcgaacttga
     6721 aagccgcgaa ccgtttgttg cacgaagcct gaaattattt ccggcaggta tttcatttgc
     6781 cgctgatgta gaggtaatgg ctttagcaga agacggatcc tacagatcgg tcaggacatt
     6841 caggtacgac cggagcaatc cgcggataaa tgtagggccg gtacctgaag caccagtggc
     6901 ggtttcactt ccggaaactt cgtcaaccag gttcaggatg atactcaata acttcagtct
     6961 tagcaactat cttacttccc gacccgacaa tgcctcggat gccggactgt cagagattgt
     7021 cctttcaggt gtaccggttg ttgaaagcta tgtggaaaaa caacttggca aaatgtgcca
     7081 gacaccattt cctttgtgga acgagtatca gtggatacca cagcaggaaa gtggtagcgg
     7141 ggaaatgaag attaatccta tggaagtggt ggatttgaca tccgggatgt cagccgacgg
     7201 gaccctggac tgggatgttc caggaggaaa gtggatcatc ctgcagatcg gggccgttcc
     7261 caccggggcc atgaatgcac aggcggcacc caatgctgtg ggatgggaag tcgataaaat
     7321 gaacagggat catctgaaaa ggcattttga cgcctacatc ggcaatctta tacgaagaat
     7381 gtctccggat gagaggacag cattaaaaca tgttgttctt gacagttatg agcagggatc
     7441 gcagaactgg actgagggaa tgatcgggga tttcatggcg aggtatgaat ataatcctgt
     7501 cccttggttc cctgtactga caggcagggt ggtggggtca gcagaccagt cggaccgttt
     7561 cctttgggac tggaggaggc tggtcgcgga ccggattgca tatgattatg ttggaggttt
     7621 ccgtgaactg tgtgcagcaa acggactgag gatatggctt gagaattacg ggcactgggg
     7681 ttttccttca gagttcctga tgtacggagg gcagagccat gatgtagccg gggaattctg
     7741 gaacgagggt gaactcggaa acattgaatg ccgtgccgca tcatcggcag ctcatattta
     7801 cggtaaaagg ctggtctcgg ctgaatcata tacttcggcc gggcaggcat accagagata
     7861 tcccgcgatg ctaaaaaaac ggggggactg gagttatacc gaagggatta accaggtagt
     7921 cctgcatctg tatatccatc agccctatga agataaaaat cccgggatca atgcatggtt
     7981 cgggacagaa ttcaacagga agaatacctg gttcaggcag tcgaaacact ggatcgatta
     8041 ccagcggagg tgcatgttta tgctgcggag aggactcccg gtaaatgatg tctgttattt
     8101 tatcggggag gatgtaccga agatgacagg ggtcagaatc ccggaacttc cccctggata
     8161 ttctttcgat tacataaatg cggaagtgat acttgacagg ctgaccgtca aagatggcct
     8221 tcttgtcctt cccgatggca tgagctacag gctcatggtg cttccaccgc ttgaaaccat
     8281 gcggcccgag gtacttaaaa agatcggtat gcttgtggaa aagggggcaa agatatacgg
     8341 acccccgcct tccaggtcgc ccagtctgca aaactatcct gaagctgaca ggacagtaca
     8401 aagacttacc ggggaattat ggcagaccga gaaggctgac aagccctccg gaggttacgt
     8461 gatggataac atgagcctgg aggaagctct gaatagtgcc ggagtacagc ccgacgtcag
     8521 cctgcctgaa agtactcctg tattgtggat ccacaggagg atgaaggatt gtgatattta
     8581 tttcattacg aatcagagcg atgaaaggat aagtttcgac gcgggtttca gggtaggcaa
     8641 gatgcagccc gaactgtggg atcctttgaa cgggatgata cgtgatttag gtgaattttc
     8701 tcacacgagc ggcatgacgg taaccccgtt acagtttgaa ccttgcggaa gcgcattcat
     8761 tgtttttaga aaaggttcag gagacaggtc agaaaaaccg gtgggaccca caaacttccc
     8821 tgttcccgtt acccttgtag agattaacac cccgtgggaa gtgacgtttg aaagaaaacc
     8881 caatggacct gaaaaaagca taatgatgga aattcccgaa gactggtcaa agagcctgga
     8941 tgaaaggatc aggtattact cagggagtgc catatatagg aacagtttta cacttggtcc
     9001 gattccggct ggagagaacg ttataattga tctcggaaaa gtaggtgtaa tggcggaagt
     9061 gaaggtaaat ggaagatttg cgggaggcac atggacccag ccgtggtcag tcgatataac
     9121 agaaatggtt aaacagggtg aaaacacatt ggagatagag gtggtgaaca actggataaa
     9181 ccggcttatc ggcgatagca ggctccctga gaaagagagg tccacatggc tgaatgagaa
     9241 ttctgtcagg cctgatgatc cgctccaggc ctcaggattg atagggccgg tagttatcag
     9301 ttcagttaag taccggtaat tgcctgactc acttttatac aatgcgactt caaagttttt
     9361 tctgatactg gataacactg tctgaccggt ggcaccgaga aggatcactt aagacaggaa
     9421 aaagaag
//
LOCUS       DAOOYG010000099         9281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_8132_length_9281_cov_7.542272, whole genome shotgun sequence.
ACCESSION   DAOOYG010000099 DAOOYG010000000
VERSION     DAOOYG010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8132_length_9281_cov_7.542272"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2111)
                     /gene="infB"
                     /locus_tag="PK288_08645"
     CDS             complement(<1..2111)
                     /gene="infB"
                     /locus_tag="PK288_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPM19032.1"
                     /translation="MTEDIKATRLSKAAREFNVGISTIVDFLHKKGFDLDSNPNTKLP
                     HEAYLLLLKEYSSDLNVKKESEKLILKDLHRKKETVSIDDIADKNITEDHERDEEVLI
                     KDTLAPKKTVDIKTEIRKSDIKLVGKIDLEKALKPKIQKPEPAREAVEKKAEQESIQP
                     EIKKKVAVEKEKVTPAEETKEAGTKPGIDLQIVGKIDLDAITGERIVQRKGEKAAVKE
                     KQAAGDTESAELIKESTAAAPAATKEPLTAVPDSEPLVAETKVAEEEDIIELFKPEIK
                     KLSGLTVVGKIDLPVEEKKKPVPYQQVSPEGESDFRRKKKRKRISKEKEQVPLEKPAP
                     VERKDKVGKRVIRKRAVHTEVDEEEVQKQIKETLARLTTKGKSKGSRYRREKRELISQ
                     RHREVEDKLEQEKNILKVTEFVSVNELATMMNIPVTEIISTCMNLGLFVSINQRLDAE
                     TMALLADEFGFRVEFVSAELQEAVREIEDDEKDLKPRPPIVTVMGHVDHGKTKLLDYI
                     RRTNVIAGEAGGITQHIGAYSVTLDDGRHITFLDTPGHEAFTAMRARGAQTTDIAIIV
                     IAADDGVMPQTVEAINHASAAGVPIVFAINKIDKPTANPEKIKEALANMNYLVEDWGG
                     KYQSQEISAKNGLNIDLLLEKVLLEAELLELKANPDKPAIGTIIESSLDKGRGFTATV
                     LVKAGTLRVGDIMIAGSCYGH"
     gene            complement(2168..3406)
                     /gene="nusA"
                     /locus_tag="PK288_08650"
     CDS             complement(2168..3406)
                     /gene="nusA"
                     /locus_tag="PK288_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPM19033.1"
                     /translation="MENVNLIDTFSEFKELKNIDRPTMMSVLEDVFRSMLAKKYGSAD
                     NFDIIVNIDKGDFEIWRNRVVVEDDELVDPNTQISLSKAKEIDEDYEAGEEVSDEVKF
                     LDFGRRAILSLRQNLTARILDLEKNNIYIKYKDRIGEIVTGEVYQVWKREILVLDDDG
                     NELIIPKNEQIPSDHFRKGDTIRAVVIKVEMKNNTPFIILSRTSPVFLERLFELEVPE
                     IFDGLITIKKIVRVPGERAKVAVESYDERIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INYTSNNQLFIQRALSPAKITSIKLIEETKRAEIYLKPNEVSLAIGKGGLNIKLASQL
                     TGYEIDVYREPGGEDEEDVNLEEFSDEIEEWIIDELKSIGCDTARSVLNLSISDLVKR
                     TDLEEETVKEVVNILKAEFE"
     gene            complement(3426..3878)
                     /gene="rimP"
                     /locus_tag="PK288_08655"
     CDS             complement(3426..3878)
                     /gene="rimP"
                     /locus_tag="PK288_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HPM19034.1"
                     /translation="MIDKDRIEKLVLEHIKGKELFLVGIKVSSSNRINVLVDTMEGIT
                     IDECADLHRHIERGLDRNVEDFELQVSSPGLDMPFAVIEQYRKNEGKKVAVLTLDGVK
                     HSGILKNVTNGGFELTTEIKVRGKAGEKKDLSFNYDQIKSVKEILIIK"
     gene            4342..5283
                     /locus_tag="PK288_08660"
     CDS             4342..5283
                     /locus_tag="PK288_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPM19035.1"
                     /translation="MDFHYYTGEEIRLLSECTLCPRECGVNRFGGGTGYCKSDAGMNV
                     ASICIHRGEEPAISGPEGICNIFFSGCNLRCIYCQNYEISNPCRRIRIKSPGYDEVLD
                     RIAALLKGGVKAVGFVSPSHVIPQVKAIIRGLHAKGYRPVTVFNTNGYDRKSTISSLE
                     GLIDVYLPDLKYIDPNSAEEFSGAPDYPETAMNAIREMYFQKGSVLITDSEGRAENGL
                     IVRHLVLPGHADDSMELLERLARDVSRGIHISLMSQYHPVPAVRHHPDLGRSLYASEY
                     SAVAKYAGELGFRNGWFQGLESHGNYRPDFSREHPFE"
     gene            5374..6627
                     /locus_tag="PK288_08665"
     CDS             5374..6627
                     /locus_tag="PK288_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPM19036.1"
                     /translation="MAYTKNKRKIINDPVYGFIGIPSELIYDLIEHPWFQRLRNIKQL
                     GLTSFVYPGACHSRFQHCLGALHLMENAIRTLRSKDILITPEEEEATLAAILLHDIGH
                     GPFSHTLEKSLVSGITHEDMSLLLMNKLNEEFDGRLSLAIDIFRGDYERKFLHDLIAS
                     QIDMDRLDYLRRDSFFTGVIEGSVGSDRIIRMLNVADDELVIDEKGIYSLEKFLIARR
                     LMYWQVYMHKTVLASENLLIKILKRAKETAASGGDLYATPSLRFFLRNNIGPSDLGTD
                     GLFTPGIVAFNFTRLDDSDIYSSIKYWVDSSDRVLSDLSRRLLTRNLYAVEMENNPFP
                     EERLSALAGKACRILDIDHEQVSNYVFTGTVSNLTYVTEGPGVRILLKNGNTADITTV
                     SDIFEHSLFSGRNTKYFLCYPKECR"
     gene            6638..7678
                     /gene="lpxD"
                     /locus_tag="PK288_08670"
     CDS             6638..7678
                     /gene="lpxD"
                     /locus_tag="PK288_08670"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPM19037.1"
                     /translation="MELNAETIAGFLGGTVDGNPEIKVSTLAKIEEGYPGALSFLANP
                     KYEHFIYSTKSSIVLVNKDFVATSPIEATLIRVDNAYDAFASLLRMVDQARPRKKGIH
                     PTAIIEEGVRLGQDLYIGAYVYVGSNCSIGDGCSVYPHAYIGDGTSIGNNSTIFAGVK
                     IYHDCIVGEGCVIHAGTVIGSDGFGFAPQSESEFMKIPQIGNVIIEDHVEIGANVAID
                     RATMGSTIIHKGVKLDNLIQIGHNVEIGENTVMAGQTGVAGSTKIGRNCMFAGQVGVV
                     GHIKIADGTRIGAKAGVSGEVRQMNTTILGTPAIDHREFLKSAVIFRKLPEMRSSIAQ
                     LEKEIESLKKSR"
     gene            7751..9145
                     /locus_tag="PK288_08675"
     CDS             7751..9145
                     /locus_tag="PK288_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPM19038.1"
                     /translation="MSEKQRTIANTVSLKGKGLHSGIDVNITFRPAPANHGYKFCRTD
                     LPGAPLIDALADHVTETSRGTTLVQNNASVSTIEHVLAALYGLRIDNALIELDGPEAP
                     IMGGSAWKFTQALKSAGFVEQKDERNYFRVKQKITYSDEEHGVDLIVYPDDHLSINVL
                     IDYNSKILGNQYAILDSIDDFEQEISKSRTFVFFHELEPLHKMGLIKGGDLDNAVVIL
                     EKEVEQSEIDRLARIFNRPGINKHTAGILNNTELFYPNEPARHKLMDVLGDLALAGQP
                     IKGKVVATRPGHYANTRLAKLIRQEIKKSQTSLNIPVYDPSVPPVYNTEDIKKRLPHR
                     YPFLLVDKIISLDDNTVVGVKSVTINEPFFQGHFPGEPVMPGVLLVEALAQSGGLLVL
                     SRVDEPERYSTYFVKIDKLKFKHKVVPGDTLILKMELIEPLRRGIVAMYGQAFVGNHL
                     VVEGEMMAQVIKNK"
     gene            9152..>9281
                     /locus_tag="PK288_08680"
     CDS             9152..>9281
                     /locus_tag="PK288_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HPM19039.1"
                     /translation="MISNLAFVHPDAILGENVVVEPFAYISGNVSIGDDSWIGPSAT"
BASE COUNT         2418 a         2145 c         2025 g         2693 t
ORIGIN      
        1 atatgtccgt agcagcttcc tgcaatcatg atgtcaccaa ccctgagggt acccgcctta
       61 acaaggacag tggctgtaaa acctcgtccc ttatcaaggg aggattcaat gatcgttcca
      121 atggccggct tgtcggggtt tgctttaagt tccagcaatt cagcttccag caataccttt
      181 tccagtagaa gatcgatgtt taggccattc tttgcagata tttcctgaga ctggtacttt
      241 cctccccaat cctcgaccag atagttcatg ttggccaggg cctcctttat cttttccgga
      301 tttgccgttg gcttatcgat cttgtttata gcaaaaacta taggaacacc tgcggctgaa
      361 gcatgattga ttgcttcaac cgtctgcggc atcactccgt catcggcagc aatgacaatg
      421 atcgcaatat cagttgtctg tgctcccctt gcccgcatgg ctgtaaatgc ctcatgaccc
      481 ggagtgtcaa ggaatgtaat gtgcctgcca tcatcgaggg ttacactgta ggcaccgata
      541 tgctgtgtga taccgcccgc ctcccccgca atcacgttgg tacgcctgat gtagtcgaga
      601 agctttgtct tgccatggtc aacgtgcccc atcactgtaa caataggcgg cctgggctta
      661 agatcttttt catcatcttc tatttccctg acagcttcct gaagttcggc acttacaaat
      721 tccactctga atccgaattc atctgcaagc agagccatgg tctcagcatc cagacgctgg
      781 tttatggata caaagagacc aaggttcata caggttgata taatttcggt aacaggaata
      841 ttcatcatgg ttgcaagctc attcaccgac acgaattccg taaccttcag tatgttcttt
      901 tcctgctcga gtttgtcctc cacctcgcga tgcctctgac tgataagctc ccgtttctcc
      961 ctgcggtatc tcgatccctt cgacttaccc ttggttgtga gcctggcaag agtttccttg
     1021 atttgtttct gaacctcttc ctcatcaact tcggtatgga cggctctttt cctgatgact
     1081 ctcttgccga ccttgtcctt ccgctcgaca ggtgcaggct tctcaagggg cacctgttcc
     1141 ttttccttgg aaattctctt tcttttcttc tttcttctga aatctgattc tccttcagga
     1201 ctaacctgct ggtagggaac tggctttttc ttctcttcaa ccggaagatc aatctttcct
     1261 accacggtca gaccagacag ttttttgatt tcaggtttaa acaattcgat gatatcctcc
     1321 tcttccgcaa ctttcgtttc tgcaacaaga ggttcggagt caggaacggc tgtaagcggc
     1381 tctttggtcg ccgccggggc agcagctgtg ctttccttta tcaattcagc cgactcagta
     1441 tcacctgccg cctgtttttc cttcaccgca gccttttctc ccttccgctg cacgattcgt
     1501 tcaccggtta tcgcatcaag atctattttc ccaacaatct gcaagtcgat gccaggtttg
     1561 gtgcctgctt cctttgtttc ctccgccggc gtaacttttt ccttttcgac tgctaccttc
     1621 ttttttattt cgggttgaat gctctcctgt tctgctttct tttcaactgc ttccctggca
     1681 ggttccggtt tctgtatttt tggttttaat gccttctcaa gatcaatctt tccaaccagc
     1741 ttaatgtcag atttcctgat ctcggttttt atgtcaacag ttttctttgg tgcaagagtg
     1801 tcctttatca gaacttcttc atccctttca tgatcttccg tgatgttttt atcagcaata
     1861 tcatcaatgg acacagtttc ctttttcctg tgaagatcct taaggatcag cttttccgac
     1921 tctttcttga cattcagatc gctgctgtat tcctttaaaa gcaacagata ggcctcgtga
     1981 ggtagtttag tgttgggatt ggaatcaagg tcaaatccct tcttatgcag gaaatccacg
     2041 attgtggaaa ttcccacatt gaattcacgg gcggccttgc tcaatcttgt tgccttgata
     2101 tcctctgtca tatatttacc ttgcctttct taatacttca aaattcgttt atcgtgttaa
     2161 aacctgatca ttcaaattca gccttcagta tatttaccac ttccttaact gtttcctcct
     2221 caagatccgt ccttttaaca agatcgctta tcgacaggtt gagaacactt ctggccgtat
     2281 cgcagccgat tgatttcaat tcatcgatta tccattcctc aatttcatcg ctgaactctt
     2341 ccagattgac atcttcctca tcctctcctc ccggctccct gtaaacatcg atctcatatc
     2401 ccgtaagctg gctcgcaagc tttatattga ggccgccttt tccaatggcg agggaaacct
     2461 cattgggttt gagataaatc tctgcccttt tggtttcttc aatcagttta atggaagtta
     2521 tctttgccgg gctcaaggct ctctggatga ataactggtt gtttgaagtg tagttgataa
     2581 catcaatgtt ctcattgcgg agttccctga caatgccgtg aatcctggaa cccttcattc
     2641 ccacacaggc accgacggga tcaatgcgct cgtcatatga ttcgactgca accttggccc
     2701 gttcaccggg tacccttact atctttttaa tggtaatgag tccgtcaaaa atttcaggta
     2761 cctccagttc aaacagtctt tctagaaata cgggagatgt gcggctgagg atgataaaag
     2821 gagtgttgtt cttcatctca accttgatga caaccgcgcg tatcgtatcc cctttccgga
     2881 aatgatccga aggaatctgt tcattcttgg gtataatcag ttcattgccg tcatcatcaa
     2941 gtacaagtat ttcccgtttc caaacctggt aaacctcccc ggtgacaatt tccccgatcc
     3001 tgtccttgta ttttatgtaa atattgttct tttcaagatc aagtatcctg gcagtcaggt
     3061 tctgcctgag cgacaatatt gccctcctgc cgaaatcgag aaatttcacc tcatcggaaa
     3121 cctcctctcc tgcctcatag tcttcatcaa tttccttggc ctttgacaga gagatctgtg
     3181 tattggggtc aaccagttca tcatcctcaa caacaaccct gttcctccaa atctcaaagt
     3241 cacccttgtc gatattgaca ataatgtcaa aattatcggc ggagccgtat tttttcgcca
     3301 gcatgcttct gaagacatcc tcgagcacgc tcatcattgt ggggcggtca atattcttaa
     3361 gctccttgaa ttccgaaaag gtatcgatca aattaacatt ttccatctgg ttaatcctct
     3421 tgaaattatt ttattattag tatttccttt actgacttga tctggtcata gttaaagctc
     3481 agatccttct tttccccggc ttttcccctg actttaattt ctgtcgtaag ctcaaagcca
     3541 ccatttgtaa cgtttttaag tatcccgctg tgttttacac cgtcgagtgt taaaaccgca
     3601 accttcttcc cttcgttctt ccggtattgt tcaatcactg caaacggcat atcgaggcct
     3661 ggggacgata cctggagttc aaaatcctcg acgttgcggt cgagtcccct ttcaatatgg
     3721 cgatgcaggt cggcacactc atcaatggtt atgccttcca tggtatcaac aagaacgttt
     3781 atcctgtttg agctgcttac ttttatgcca accaggaaaa gctcctttcc cttaatgtgt
     3841 tccaggacca gtttttctat tctatccttg tcaatcatct tccgaaaaac gataataaaa
     3901 taggggactc cggtccccta ggtttcaatt acccctcttt tcaggtgcaa aggtaagtaa
     3961 tgttttgaaa actgcaagct tttagtttaa aaaactgcca catcattagc ccgttatgat
     4021 gattttcact gaaagtccat tagctgattc tgaccatttc cttatgtctg gcgaaaatca
     4081 gtaacataga ccaggattct aatttatcca tttaacctgc agatatttag catcatacaa
     4141 aaacacggcc tgggaggaaa gatgttttta tctgacgatc tgaattcccg gactttcccc
     4201 tgatggtttt cagcagaatt ttttattcct ttatgccagg cagggattgt tgcctattta
     4261 tcatggaatg ttgcatgcag cggggcataa agatacattt tcatgattta atgctttcat
     4321 ccgcagttga tcacgattta aatggacttt cattactata cgggagagga aatcagactg
     4381 ctttcagaat gcaccttatg tccgcgcgaa tgtggcgtaa acaggttcgg aggcggtaca
     4441 ggctattgca aatcagatgc tggaatgaac gtggcttcca tttgtattca caggggcgag
     4501 gagccggcga taagcggtcc ggaaggaata tgtaatattt tcttttcagg atgcaatctc
     4561 cgctgcattt actgccagaa ttatgagata agcaatccat gcagaaggat caggataaaa
     4621 tcacccggtt atgatgaagt tcttgataga attgcagccc tgttaaaagg aggagtaaaa
     4681 gcagtgggct ttgtttcccc atcacacgtc attccccagg tcaaagctat tatccgcggc
     4741 ctgcatgcaa agggatacag gccggtaaca gtgttcaata caaacggcta tgacaggaag
     4801 tcaactatca gcagtcttga ggggttgatt gatgtttacc tgcccgactt aaaatatatt
     4861 gatcctaatt ctgcagaaga attttcaggt gcgccggatt atcctgaaac ggctatgaat
     4921 gcaatcaggg agatgtattt ccagaaagga tcggtattga tcacagattc ggaaggcagg
     4981 gctgaaaacg gactgattgt acgccatctc gtgctgcccg gtcatgctga tgacagcatg
     5041 gaattactgg agaggcttgc ccgtgatgtt tcgagaggca ttcacatatc gctaatgtcc
     5101 cagtaccatc cggttcctgc tgtgaggcat catcctgatc ttggaaggtc gctttacgcc
     5161 tccgaatatt ctgccgttgc taaatatgcc ggggaacttg gattccgtaa cggatggttc
     5221 cagggtttgg agagccatgg gaactacaga ccggatttca gccgcgaaca tccgtttgaa
     5281 tgaataacaa ccggaaatgt taaacaggga tcattgtcaa tcatatttgc agtgacaaac
     5341 aaattcataa atttgtagcc aaagaatttt cgggtggcct atactaaaaa caagagaaaa
     5401 ataatcaatg atccggttta tggatttatt ggcataccca gcgaactgat ctatgacctg
     5461 attgaacatc cctggtttca gcgattaaga aacataaagc aacttgggct tacgagcttc
     5521 gtttacccgg gtgcctgcca cagcaggttc cagcactgcc tgggagcgct ccatctcatg
     5581 gaaaatgcaa taaggacact caggagcaag gatattctga ttactcccga agaagaagaa
     5641 gccacacttg cagcaattct tctccatgat atagggcacg gaccgttttc ccacactctt
     5701 gagaaatcgc ttgtcagtgg aataacccat gaggatatgt cgcttctgct gatgaataag
     5761 cttaacgagg agttcgatgg gcggttatcc ctggccattg atattttccg gggtgattac
     5821 gaaaggaagt ttttgcacga tcttattgca agtcagattg atatggatcg acttgattac
     5881 ctcaggcggg attcattctt taccggggta atagagggct cggttggatc agacagaata
     5941 atcaggatgc taaatgttgc agacgatgaa ctggtaatag atgagaaagg tatttactcg
     6001 cttgaaaagt ttctgatagc ccgccggctg atgtactggc aggtttatat gcataaaacc
     6061 gtactggcgt cggagaatct tctgattaaa atactcaagc gggccaagga gaccgccgct
     6121 tcaggaggtg atctgtacgc cacaccctcg ctcagatttt ttcttcgtaa caatatcgga
     6181 ccctccgatc tcggaactga cggattgttt acacccggaa tcgtcgcgtt caatttcaca
     6241 agacttgatg attctgatat ttattcatcg atcaaatact gggtcgacag ctctgaccgg
     6301 gttctttccg atctgtcacg gcgtttgcta acccgcaacc tctatgctgt tgaaatggag
     6361 aataatcctt ttccggaaga gaggctcagc gcacttgccg ggaaagcctg ccggattctg
     6421 gatattgatc atgaacaggt aagtaattat gtttttacag gaaccgtttc aaacctaaca
     6481 tatgtaacgg aaggacccgg cgtaaggatt ttgttaaaaa acggaaatac cgccgatatt
     6541 actaccgttt cagatatttt tgaacatagc ctgttttcag gcagaaatac aaaatacttc
     6601 ctgtgttatc ccaaagaatg cagataaaag aaatattatg gaattaaatg cggaaacaat
     6661 agcaggattc ctcggaggaa cagttgatgg aaacccggag atcaaagtaa gcaccctggc
     6721 aaaaattgag gagggatatc cgggagccct ttctttcctg gcaaatccca agtatgaaca
     6781 ttttatttat tcaacaaaat cttcaattgt gcttgtcaat aaggattttg ttgcgacatc
     6841 gccgattgaa gccaccctta tcagggtcga taatgcatat gatgcctttg cctcattgct
     6901 caggatggtc gatcaggccc ggccccggaa gaagggaata catcccactg ctataataga
     6961 agaaggtgtc aggctcggcc aggacctata tatcggggca tatgtatatg tgggttctaa
     7021 ttgcagcata ggcgacggat gctcggttta tccacatgct tatataggag acgggaccag
     7081 tattggaaac aacagcacca tatttgcagg ggttaaaata tatcacgact gtattgtagg
     7141 tgaaggttgt gtcatccatg cgggaacagt aatcgggagt gacggtttcg gatttgctcc
     7201 ccaatcggaa agtgaattca tgaaaatacc ccagataggc aatgtaatta tcgaggatca
     7261 cgtggaaatt ggagccaatg ttgccattga cagggcgacc atgggttcaa ccataataca
     7321 caagggtgta aagctggata atcttataca gatcggacat aatgttgaaa taggtgaaaa
     7381 tacagttatg gccggacaga caggcgttgc aggttcaacc aagatcggaa ggaattgcat
     7441 gtttgccgga caggttggcg ttgtgggtca tatcaaaatt gctgacggaa ccagaatagg
     7501 ggctaaagca ggtgtctccg gcgaagtcag gcagatgaac accactattc tgggaactcc
     7561 tgcaattgac catcgtgaat ttcttaaaag cgccgttatt ttccgtaaac tgcccgagat
     7621 gcgatcatca atcgcacagc tggaaaagga aatagaatcc ctgaagaagt ccaggtaatt
     7681 atccttttgg attgttcggt tttttttgat ttttttgtac cctgaaaaat tagaaacgat
     7741 tccttccgac atgagtgaaa agcaaaggac cattgcaaat acagtcagcc tgaaaggcaa
     7801 agggttacac agcggaatag atgtaaatat cacattcagg ccggcaccgg caaatcacgg
     7861 ctataaattc tgccgcaccg accttccggg tgcaccgtta attgatgccc tggccgatca
     7921 tgtaactgaa acatccaggg ggaccacact ggtgcaaaac aatgcttctg tatcaactat
     7981 tgaacatgtg ctagctgcac tttatggctt gagaatagat aatgcactta tcgagctcga
     8041 cggacccgaa gctccaataa tgggcggatc cgcctggaaa ttcactcaag cgctgaaatc
     8101 tgccggcttt gttgaacaga aggatgaaag gaattatttc agggtaaagc agaaaattac
     8161 ctactccgac gaggaacatg gtgtcgacct gatcgtgtat cctgatgatc atctgagtat
     8221 caatgttctg atcgactata attcaaaaat ccttggcaat caatatgcga ttcttgattc
     8281 aatcgatgat tttgagcagg aaataagcaa aagccgcaca ttcgtgttct ttcatgagct
     8341 tgagccgctc cataagatgg gtttgatcaa gggcggggac ctcgacaacg cagtggtcat
     8401 cctggaaaaa gaagttgaac agtcggaaat tgacagactg gcacggattt ttaacagacc
     8461 cggtataaac aagcacacag cgggtattct gaacaatact gagcttttct accccaatga
     8521 accagcccgg cacaaactga tggacgtgct tggagatctt gctcttgcag ggcagccaat
     8581 aaagggaaag gttgttgcta cccgccccgg acattatgcc aatacccgac tggcaaaact
     8641 tatcagacag gaaataaaga agtcgcaaac cagtctgaac attccggttt atgacccttc
     8701 ggtcccgcct gtatataata ctgaggatat caagaaaaga ttacctcaca gatacccgtt
     8761 tttgctcgtt gacaagataa tctcactcga tgacaacaca gttgtgggtg taaaaagtgt
     8821 tacaatcaat gaaccgttct ttcagggcca tttccccggg gaaccagtaa tgccgggcgt
     8881 tcttctggtt gaggctcttg cacagtcggg ggggttgctt gttctgagca gggttgacga
     8941 accggaaaga tattccacct attttgtgaa aatcgacaaa cttaaattta aacacaaggt
     9001 agtacctggc gataccctga tactgaaaat ggaactgatt gaacctctga gaagagggat
     9061 agtggccatg tacggacagg catttgtggg aaatcacctg gttgtggagg gggaaatgat
     9121 ggcccaggta ataaaaaaca aatagctgaa catgatttca aatttggctt tcgtccaccc
     9181 tgatgcgatt cttggagaaa atgttgttgt tgaaccattt gcttatattt ccggaaatgt
     9241 atcaattgga gatgactcct ggatcggccc cagtgcaaca g
//
LOCUS       DAOOYG010000100         9201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_8237_length_9201_cov_4.615351, whole genome shotgun sequence.
ACCESSION   DAOOYG010000100 DAOOYG010000000
VERSION     DAOOYG010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8237_length_9201_cov_4.615351"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /locus_tag="PK288_08685"
     CDS             <1..1721
                     /locus_tag="PK288_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPM19040.1"
                     /translation="FFSSGLMSAGDGNAISGCIRISAGAVASDASRYIIFGIDNLSRL
                     IVAAAGFFAFIISLYSLVYNKINKVRNFNSWFLITVGCSFGALLSDNLLLFLTFWGIL
                     GITLYMLIPGRNEESSAAAKKTLILIGASDSIMIIGIALLWWLTGSLSMNSISVPTDN
                     TPGALAFLTLLAGSFTKAGAFPFHTWVPDYTKDAPASSSSLLPASLDKLLGIYLLARL
                     TRDMFILSQWLTFLLLLTGVITITGAVMMALIQHNYKKLLGYHAVSQVGYMILGFGLG
                     SPIGIAAGLFHMINHAIYKSGLFLAAGSIEKQTGKEDIDDLGGLSGAMPVTFAATLVF
                     SMSISGVPPFNGFASKWMIYQGIIDFGNGPGFVNQLWIVWLGLAVLGSALTLASFIKL
                     TGGIFLSRRKQEFSGIREVPALMWIPMAVLALLCITFGVLATNLIVPKLFTPVSGDFV
                     FTGFWDSTFVSILVLISILLGIVIYLVSGLKRFRREDSFIGGERIQDQAAYPTPEYYK
                     TISEMDFPAWMYDKAGKKWFDIYDLSRRFVLWLSRQFSLAHDGVLSGYALWVFAGLII
                     LLLVMI"
     gene            1736..2263
                     /locus_tag="PK288_08690"
     CDS             1736..2263
                     /locus_tag="PK288_08690"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPM19041.1"
                     /translation="METALSIIITLMILGAIYALHARDLLSAVIACGIVGYGLVICFL
                     LLKAPDLAIVQIVVETITLIIMVAVVLDSSRQEPFVKPDLQGYIAIVSGLLLIILLFH
                     FFRISTSALDDFGRTTLRMSEAYIKGAADRTGSANLVTGVVFDFRGYDTLGEATVLVT
                     AVIGVLTILRIRGKK"
     gene            2407..2835
                     /locus_tag="PK288_08695"
     CDS             2407..2835
                     /locus_tag="PK288_08695"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HPM19042.1"
                     /translation="MTTIVKKTTQLMAGLVFLYGIYIIIHGHLTPGGGFAGGVIAAGS
                     FILIILAYGSDFIRLTRKEHGSTMYENLAILIVAFLALSGLFTGACVFFLNWLPRGTT
                     GELASAGMLPLYNIFVGIEVASSIVTIFLALVIFKEEMSE"
     gene            2832..3170
                     /locus_tag="PK288_08700"
     CDS             2832..3170
                     /locus_tag="PK288_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HPM19043.1"
                     /translation="MIVYCLCFVLFLTGLYGVLTRRNLVKIAISLSLMECSLFLFLAL
                     IGYTEGGIAPIVDPADPVKNYVDPLPQALVLTAIVIGLATTAMLMAVIIRLYRKYGTF
                     DIREIKELRG"
     gene            3174..4712
                     /locus_tag="PK288_08705"
     CDS             3174..4712
                     /locus_tag="PK288_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPM19044.1"
                     /translation="MNPLIPLFVMIPLAGAFLIMITGRFAVWFGRYLTTLILTVLAAF
                     SIFSLAVTGENTAVYKVGGWEPLNGIPIGIHMVMDGFSVIVLCIINIVGVLAALYSIP
                     YIKRYTSENYYYSLFCLMIGGMNGVVLSGDIFNIFVFLEVSAISSYALVAFGVEKNEL
                     EASFKYQVLGALASFLILLGIALIYWKTKTLNIADIREVFSTGYDRTYYLFVQILLLG
                     GFGLKAAMIPFHAWLPDAHSSAPSPISAMLSGVFIKAVGIYVIIRLFFNMFIVTENIS
                     ILITVMGTLSMAVGVFLAIGQWDIKRLLAYHSISQMGYVILSTGIGMILIARGKSPEI
                     AALAIAGGLFHLVNHAAFKGLLFFNAGAIEYSTGTRDLQEMGGLAGSMPATHATSFIA
                     SMSISGIPPFNGFFSKLIIIIAAIMAGFYLLAALAVVVSIITLASFLKFQRYAFYNKP
                     ENKDDKKNKEVPLPMVFSMVIMGILCILLSLLAFPGIRETILEPAINILTDPLKYSSL
                     ITGS"
     gene            4714..5199
                     /locus_tag="PK288_08710"
     CDS             4714..5199
                     /locus_tag="PK288_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPM19045.1"
                     /translation="MRYLAVFILSLVFWLLLTFSLKIPNLIIGGLASLLSAAIFSDMY
                     FKTPSKLLQPHRYFWFLIYLAVFIVECIKANFDVAYRVLHPAMPIKPGIVKIRTTLRS
                     DFARTILANSITMTPGTITVDLIGDEFYIHWIFVRSEDPEIYTGMITGLFEKYIKRFA
                     E"
     gene            5196..5462
                     /locus_tag="PK288_08715"
     CDS             5196..5462
                     /locus_tag="PK288_08715"
                     /inference="COORDINATES: protein motif:HMM:NF015993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HPM19046.1"
                     /translation="MTEFLTVLLYIQIALSAACLYRIIIGPTIPDRMVGIDIFGILVV
                     GICAIIAVQTEKSFILDIGIAWVILSFIGTLTLAKYLSGKKLNE"
     gene            5455..5781
                     /gene="mnhG"
                     /locus_tag="PK288_08720"
     CDS             5455..5781
                     /gene="mnhG"
                     /locus_tag="PK288_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPM19047.1"
                     /translation="MNDMITVILICTGVLFNIFGCIGLIRLPDVYNRLQSATKCVTLG
                     CCSILLGALIHFGIGSAGMKALIAIPILFFSATVAAHALIRGAYHFGTGLDKRTVKDD
                     YKNAVK"
     gene            5778..6398
                     /locus_tag="PK288_08725"
     CDS             5778..6398
                     /locus_tag="PK288_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPM19048.1"
                     /translation="MKYPKIRELREAVVSLFTPAYTSKFPAEPHVPFEKFRGKPVVDD
                     ANCVGCETCTNVCPPGAITFTDDKEKQIRILRRDYGKCIFCGQCQDHCITGKGVILSD
                     KIYDIAVFDRDKVVEYQEKELVICRNCSAVITTREHLCHMHRKLGPRAFSSILNLNML
                     NEKLRLSEGQDTEVEIADELKRKDMFNVICPNCLRQVLVKYIVEGA"
     gene            6391..6843
                     /locus_tag="PK288_08730"
     CDS             6391..6843
                     /locus_tag="PK288_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPM19049.1"
                     /translation="MPELLEEILAAKDKKKLFVGVGNVLKCDDGTGVYISNRIVPRND
                     ISVLTVETGIGNYIGKINSIGPDILVLLDAVDMGARPGTIKTLPITGLQDMTFNTHNI
                     SLARFADFFKMEIFITGIQPEKIEFGEKISYIVRQEADRLIGIINKTK"
     gene            6859..7269
                     /locus_tag="PK288_08735"
     CDS             6859..7269
                     /locus_tag="PK288_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19050.1"
                     /translation="MTQYLCKACRGVLNVKTSIILSATKLNSSKKGLLYLNPELGNYT
                     VTTHPTFQIEEGEEYLINCPICHAHLNSLKYDHLVRIKMIDDKGEEFDIYFSDIVGEK
                     CTFKISGSTVEKIGPDAVKYNKYFDIPEEDKKYL"
     gene            7378..>9201
                     /locus_tag="PK288_08740"
     CDS             7378..>9201
                     /locus_tag="PK288_08740"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPM19051.1"
                     /translation="MLNQKAADNIRILAMAMVEKAKSGHPGGAMGGADFIHILFSEFL
                     NFDPGDPYWINRDRFFLDPGHMSPMLYSVLTLTGHFTLDDLKSFRQWGSPTPGHPELD
                     VARGIENTSGPLGQGHTMAVGAAIAERFLAARFGELFAHKTYAFISDGGIQEEISQGA
                     GRLAGFLGLNNLILFYDSNDIQLSTETSAVTAEDTSAKYRAWGWNVITIDGNDHNQIR
                     EALTNACGEKERPTIIIGRTIMGKGLLDNEGKSFERKTSTHGMPVSEAGGSFGKSVKN
                     LGGNPDDPFMIFDEVKDLYKGILNKKRKLAEEWKEKKKKWAEENRESAQRLEMYYSGK
                     IPETDFSSVVQKEGSATRAASSAVLGMLAQKLGNMIVASADLSNSDKTDGFLKHTKAF
                     SGGDFSGSFLQAGVAELTMAAVMNGMALHGGVIPACGTFFVFSDYMKPAVRLACLMEL
                     PVRYIWTHDAFRVGEDGPTHQPVEQEAQIRLMEQLRNHSGINSMLVLRPADANETTVA
                     WKMALENTRTPTALILSRQNIKDLPSPSNNRYSDALQLEKGAYIVSDCEGKPDIILIA
                     NGSEVSTLFEGAELLKKHNLRIRIVSAPSEGLFRNQSIEYQK"
BASE COUNT         2432 a         2123 c         2224 g         2422 t
ORIGIN      
        1 tgtttttctc ttccggcctg atgtcagcag gggacggaaa tgcaataagc ggatgtatca
       61 ggatatccgc aggtgccgtg gcatcggatg catcaaggta tatcattttc ggtatcgaca
      121 atctcagcag gctaatagtg gccgcagcag gtttttttgc ctttataatc agcctgtatt
      181 cacttgtata taacaaaatc aataaagtca ggaactttaa ttcctggttc cttatcactg
      241 tcggctgttc gttcggagca ttgctttccg acaaccttct gcttttcctt accttctggg
      301 gtatacttgg cataaccctg tacatgctga taccaggccg caatgaagaa agttccgcgg
      361 ctgcaaagaa aacacttatt ctgatcggag cttccgattc cattatgatc ataggtattg
      421 ccctgttatg gtggctgaca ggctccctta gcatgaacag catatcggtt ccaaccgaca
      481 atacgcccgg agccctggct tttctgaccc tgctggcagg aagtttcacc aaggcagggg
      541 cattcccttt ccatacctgg gtcccggatt ataccaaaga tgctcccgca tcctcatcat
      601 ccctgctgcc tgcatccctg gacaaacttc tcggcatcta tcttcttgcc aggcttaccc
      661 gggatatgtt cattctcagc cagtggctca cgttcctgct tctgctgaca ggtgtgatta
      721 caataacagg agccgtgatg atggcactga tacagcacaa ttacaagaaa ctgctgggat
      781 atcatgccgt ttcccaagtc ggctacatga tcctcggttt cgggctgggc tcaccgatcg
      841 gcatagctgc cggtttgttc catatgatca accatgcgat ctacaaaagc ggccttttcc
      901 tcgcagccgg ttcaattgaa aagcagaccg gaaaagaaga cattgatgac ctcgggggcc
      961 tttctggtgc aatgcccgtt acctttgctg caacacttgt attttccatg tccatttccg
     1021 gagtgcctcc attcaacgga tttgcctcca aatggatgat ctaccagggt atcatcgatt
     1081 tcgggaacgg tccaggcttt gtaaaccaac tatggatagt atggctgggg ctcgcggttc
     1141 tcggttccgc acttacccta gcaagcttca tcaaacttac aggaggcata ttcctgagcc
     1201 gcagaaaaca agaattcagc gggatacgtg aagttccggc actgatgtgg atccctatgg
     1261 cagtactcgc acttttgtgc ataactttcg gcgtacttgc caccaacctg atcgttccga
     1321 aactctttac tccggtttcc ggcgattttg ttttcacggg attctgggat tcaacatttg
     1381 tttcaatcct ggtactgatc tcaattcttc tcgggatcgt gatctatctc gtctcagggc
     1441 tgaaaagatt caggcgcgaa gacagtttta tagggggaga aaggatccag gatcaggcag
     1501 cctatcccac tcctgaatat tataaaacca tcagtgagat ggattttccg gcctggatgt
     1561 acgataaagc cgggaagaaa tggtttgata tctacgatct gtcgcgtcgg tttgtcctct
     1621 ggctaagcag gcagttcagc ctggcgcatg acggggtcct ttcaggttat gccctgtggg
     1681 tgtttgccgg actgatcatc ttgctgcttg taatgatata aacaaacagg tcatcatgga
     1741 aaccgctctg tccattatta tcacactcat gatcctggga gcgatttatg cattgcacgc
     1801 cagggatttg ctttcggccg tcattgcctg tggcattgtc ggatatggac tggtgatctg
     1861 ctttcttctg ctgaaagccc ccgatctcgc cattgtgcag atagtagtgg aaacaataac
     1921 actcataata atggtggcag tggtgctcga cagctcccga caggaacctt ttgtaaaacc
     1981 tgacctgcag ggatacatag caattgtttc agggctcctg cttattattc tcctgttcca
     2041 ctttttcagg atctcaacat ctgcacttga cgatttcggc agaacaacac tgcgtatgtc
     2101 tgaagcatat attaagggtg ctgcagacag aaccggcagt gccaatcttg taaccggggt
     2161 tgtttttgat ttcaggggct atgataccct cggtgaggca acagtgcttg tcaccgccgt
     2221 aatcggagta ctgacaatcc tcaggatcag ggggaaaaaa tgatatgaaa attgtaccat
     2281 gaaaatgact gcagacggat tatcaacaca ccttaaaccc atatatcaga cagcaatttg
     2341 aaaaaacggt aacaccggga tcagcaacat taactgaaag catataacac taaacatatg
     2401 aaggggatga cgacaatagt gaagaaaacc acccagctta tggcgggtct ggtctttctt
     2461 tacggtattt acattatcat ccacgggcat cttactccgg ggggaggatt tgcaggaggc
     2521 gtgatcgctg cgggttcgtt catcctcatt atcctggcat acggaagcga tttcatcagg
     2581 ctgaccagaa aagagcatgg atcgacaatg tatgagaatc ttgccatact cattgttgcc
     2641 tttcttgctt tatcagggct ctttaccgga gcatgtgttt tctttctgaa ctggctgccc
     2701 cggggaacca ctggtgaact tgcaagcgcg ggaatgctgc cattgtacaa tatttttgtc
     2761 gggatagaag tcgcctcgtc aatcgtgaca attttccttg cactggtaat atttaaagaa
     2821 gaaatgtcgg aatgatcgtc tattgtcttt gttttgtact gttcctgacc ggtctctacg
     2881 gtgtcctgac caggagaaac ctggtcaaga tcgccatatc cctgtcgtta atggaatgca
     2941 gcctgttttt gttccttgca ctgataggat acaccgaagg gggaattgct ccgatagttg
     3001 atcccgcgga tccggtgaaa aattacgttg atccgctgcc ccaggcgctt gtgctaaccg
     3061 caatcgtgat cggccttgcg actacagcca tgcttatggc tgtgatcatc aggctttatc
     3121 gtaaatacgg aactttcgac atcagggaaa ttaaagaatt aagggggtaa tatatgaatc
     3181 cgctgatccc gctttttgta atgatcccgc ttgccggagc cttcctgata atgataaccg
     3241 gaaggttcgc cgtatggttc ggcagatatc tgactaccct gatactgacg gtacttgctg
     3301 cattcagcat cttttccctt gctgtcacgg gtgaaaacac agctgtttac aaggtcggag
     3361 gatgggaacc gcttaacgga atccctatcg ggatacacat ggtaatggac ggcttttccg
     3421 tgattgtcct gtgtataata aacatcgtcg gggtcctggc cgccctgtat tccataccat
     3481 atataaaaag atacacatcg gagaactatt actactcgct tttctgtctg atgatcggcg
     3541 gcatgaacgg tgttgtgctg agcggagaca tattcaatat attcgtgttc ctggaggtat
     3601 ccgccatttc gtcctacgcc cttgttgcct tcggggttga aaaaaatgag ctcgaggcct
     3661 cattcaagta ccaggtactg ggagcactgg catcgttcct aattcttttg ggaattgccc
     3721 ttatctactg gaaaaccaaa accctgaata ttgccgatat aagggaagta ttcagcaccg
     3781 gatatgacag gacgtattac ctgtttgttc agatactcct gcttggcgga ttcgggctca
     3841 aggctgcaat gatcccattt catgcatggc tgccggatgc ccattcatcg gccccctccc
     3901 ccatctcagc catgctttcg ggagtcttca taaaagctgt ggggatctat gtgatcatca
     3961 ggctgttctt caacatgttc attgttaccg aaaatatttc catcctgata actgtaatgg
     4021 gtacgctttc aatggctgtt ggggtgttcc ttgcaatagg tcaatgggat atcaagcgac
     4081 tgctggctta tcacagcata agccagatgg gatatgtgat actttcaaca ggaatcggaa
     4141 tgatactcat cgcgcggggg aaaagtcctg agatcgcagc acttgcaatt gcgggagggc
     4201 tcttccacct cgttaatcat gctgccttta aaggccttct ttttttcaat gcaggagcca
     4261 ttgagtactc gaccggcaca cgcgatctcc aggaaatggg cggactggca ggttcaatgc
     4321 cggcaacgca tgcaacttcc ttcatcgcat cgatgtccat atcgggaata cctccattca
     4381 acggtttctt cagcaagctc ataataatca ttgctgccat tatggccgga ttctatctgc
     4441 ttgcagccct ggccgtcgta gtaagcatta ttaccctggc atcattcctc aaattccaga
     4501 gatatgcctt ttacaacaag cctgaaaaca aggatgacaa aaaaaacaag gaggtgcccc
     4561 tgccaatggt gttcagtatg gtaatcatgg gaattctttg cattctgctg agtctcctgg
     4621 catttcccgg gatcagggag acaatacttg agcctgcaat aaatatactc acggatcctt
     4681 tgaaatattc atcccttata acaggaagct gacatgaggt accttgccgt ttttatatta
     4741 tcactggtat tctggctgct gctgacattc agccttaaaa tacccaatct tatcattggg
     4801 ggcctggctt ctctgctcag tgcagcaata ttctcggaca tgtatttcaa aactcccagc
     4861 aaactccttc agccgcacag gtatttctgg ttcctgattt accttgcggt tttcatagtg
     4921 gaatgcatca aagccaattt tgatgtggca tacagggtgc ttcatccggc aatgcccata
     4981 aagccgggca tcgttaagat aagaaccacg ctcagatcag attttgccag gacaattctg
     5041 gccaattcaa taaccatgac gcccggcact ataacagttg acctgatcgg tgacgagttt
     5101 tatatccatt ggatctttgt caggtcagag gatccggaaa tttataccgg gatgatcacc
     5161 ggattgtttg aaaaatacat taaaagattt gcagaatgac tgaatttctg actgtactgc
     5221 tttacataca gatagcattg agtgctgcat gcctatacag gataataata gggccaacca
     5281 tacctgacag gatggtcggg attgacattt tcggcattct agtggtgggc atttgcgcaa
     5341 ttattgctgt tcagactgaa aaatcattta ttctcgacat agggatcgcc tgggtaatcc
     5401 tgagtttcat aggcaccctc acacttgcga aatatctcag cggaaaaaaa ttaaatgaat
     5461 gatatgatca ctgttatact gatatgcacg ggtgttttat tcaatatctt cggatgcatt
     5521 ggtctgatcc gcctgcccga tgtttacaac aggctgcagt ctgcaaccaa atgtgtgacg
     5581 ctcggatgct gcagtattct cctgggggcg ctgatccatt ttggcatcgg cagtgcaggc
     5641 atgaaggccc tgattgccat acccatactt ttcttcagtg caacggtggc tgctcatgca
     5701 ctgatcaggg gagcttacca tttcggaaca gggctggata aaagaaccgt taaggacgac
     5761 tataagaatg ccgtgaaatg aaatatccga aaataagaga actcagggag gcagttgttt
     5821 cactgttcac acctgcctat acatcgaaat tcccggctga accccatgta ccctttgaaa
     5881 aattcagggg aaaaccggtg gttgatgatg caaactgcgt cggctgcgag acatgcacaa
     5941 acgtgtgtcc gccgggggca attacattta ccgatgataa ggaaaaacag atacgaatat
     6001 taaggagaga ttacggcaaa tgcatatttt gcggccagtg ccaggatcat tgtattaccg
     6061 gaaagggagt catcctgtca gacaagattt atgacattgc ggtattcgac agggacaagg
     6121 tggtggaata ccaggaaaag gaactggtga tatgccgcaa ctgcagtgca gttatcacca
     6181 ccagggagca tctgtgtcat atgcacagga aacttggccc ccgtgcattt tcctcgatac
     6241 tgaatcttaa tatgctgaat gagaaactca gactctctga aggacaagat acggaagttg
     6301 agattgcaga cgaactgaaa agaaaagaca tgtttaacgt catatgccct aactgcctga
     6361 ggcaggtact ggtaaaatat attgtggaag gtgcctgaac ttcttgagga aatattagcg
     6421 gcaaaagata aaaaaaagct ctttgtcggt gtcggtaatg tcctgaagtg cgacgacgga
     6481 acaggcgttt acataagcaa caggattgtg ccgcgcaatg atatttcagt ccttactgtc
     6541 gagaccggga tcgggaacta tatcggcaag atcaattcaa tcggtccgga tatccttgtg
     6601 cttcttgatg cggtggatat gggggcaagg cccggtacaa tcaaaaccct accgataacc
     6661 ggtttgcagg atatgacttt caatactcac aatatttccc ttgcaagatt cgccgatttt
     6721 ttcaaaatgg agatatttat tacaggaatt cagcctgaaa aaattgaatt tggagaaaaa
     6781 atctcgtata ttgtcaggca ggaagcagac agactgattg gcataatcaa caaaaccaaa
     6841 taaacaggag gttaccttat gacacaatac ctatgcaagg catgcagggg agttttgaat
     6901 gtaaaaactt cgataatcct ttcagccacg aaactcaaca gctccaaaaa gggattgctc
     6961 tacctcaatc ctgagcttgg aaactacaca gtgacgacac atccaacctt tcagatcgaa
     7021 gaaggagagg aataccttat taactgtccc atctgtcatg cccaccttaa cagcctgaaa
     7081 tacgaccatc ttgtcaggat caaaatgatt gatgacaagg gagaagagtt tgacatatac
     7141 ttctctgata ttgtcggtga aaaatgcaca ttcaagatca gcggttcaac agttgagaag
     7201 attggcccgg atgctgttaa atacaacaag tatttcgata ttcccgagga agacaaaaaa
     7261 tatctgtaat cctgttaggt aatcaggctg accggttttc attgtacaat ccactatata
     7321 ttttaatttt gtttccgttc tgaccattaa cggcaggtat tttatttctt caaaactatg
     7381 ctgaatcaaa aagctgcaga caatattcgc attctggcaa tggcgatggt ggaaaaggca
     7441 aaatcgggac atcccggggg cgccatgggc ggagccgact ttatccacat tctcttttca
     7501 gaattcctga attttgatcc cggagatcca tactggataa accgcgacag gttcttcctg
     7561 gatccgggtc acatgtcgcc gatgctgtat tcggtactga cccttacggg tcatttcacc
     7621 cttgatgatt taaaaagctt caggcaatgg ggcagtccga cgccaggtca tcccgagctt
     7681 gatgttgcca ggggcataga gaacacatcc ggtccgcttg gacagggaca tactatggct
     7741 gtgggcgctg caattgccga aagatttctt gcagccaggt tcggggaatt gtttgcccac
     7801 aaaacctatg cgttcatttc cgacgggggg atacaggaag agatatcgca gggtgccggc
     7861 cgtcttgccg gtttcctcgg gctgaataat ctgatattgt tctatgattc aaatgacatc
     7921 cagctgtcga ctgaaaccag cgccgtcact gcagaggata cttctgcaaa atacagggcg
     7981 tggggatgga acgtgatcac catcgacggc aacgaccaca accagatcag ggaagccctg
     8041 accaatgcct gcggggagaa agaaagaccg acaatcatca ttggcaggac aatcatggga
     8101 aaggggctgc ttgacaatga ggggaagagt tttgaaagga aaacttcaac ccatggaatg
     8161 ccggttagcg aagcaggcgg atcgttcgga aagtcggtga aaaacctggg cggcaacccg
     8221 gatgaccctt tcatgatctt tgatgaagtg aaggatctgt acaaggggat cctgaacaaa
     8281 aaaagaaagc tggccgaaga atggaaggaa aagaagaaga aatgggcgga agagaacagg
     8341 gaatcggctc agaggctcga gatgtattat tcgggaaaga tacctgaaac agacttcagt
     8401 tcagttgtcc agaaagaagg ctccgcgaca agggcagcct cttctgccgt ccttggcatg
     8461 cttgcgcaaa aactggggaa tatgatcgtg gcttcagcgg atctttcgaa ttccgacaaa
     8521 accgatggtt ttctgaagca caccaaagcg ttttccgggg gtgatttttc cggctcgttt
     8581 ctccaggcag gagttgcaga gctgacaatg gctgctgtaa tgaacggcat ggcccttcac
     8641 gggggcgtca tcccggcatg cggcaccttt tttgtttttt ctgattatat gaagccggct
     8701 gtaaggctgg cgtgcctgat ggaacttcct gtcaggtaca tctggacaca cgatgccttc
     8761 cgtgtcggtg aggacggccc gacgcatcag cctgtggaac aggaagcaca gatccgcctg
     8821 atggagcagc tccggaatca cagcggaatc aacagcatgc ttgtcctcag accggctgat
     8881 gcaaatgaga ccacggtggc atggaaaatg gcgctggaaa acacacgtac ccctactgcc
     8941 ctgatcctta gcaggcaaaa catcaaggat ctgccttcac cgtcgaataa ccgatattcg
     9001 gatgcactgc agctggaaaa aggtgcttac atagtgagtg actgcgaagg caaaccggat
     9061 ataatcctga ttgccaacgg ttcggaagtc tcgaccttgt ttgagggggc cgaactgctt
     9121 aaaaaacaca atctcaggat caggatagtc tcggctcctt ccgaaggact gttcaggaat
     9181 cagagtatag agtaccagaa g
//
LOCUS       DAOOYG010000101         9183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_8267_length_9183_cov_5.986525, whole genome shotgun sequence.
ACCESSION   DAOOYG010000101 DAOOYG010000000
VERSION     DAOOYG010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9183
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8267_length_9183_cov_5.986525"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1060
                     /gene="tpiA"
                     /locus_tag="PK288_08745"
     CDS             305..1060
                     /gene="tpiA"
                     /locus_tag="PK288_08745"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPM19052.1"
                     /translation="MRKKIVAGNWKMNMELAEGLKFASELDAYFKEKPAAAGVVLCTP
                     FIHLAGAADILKGGNVALGAQNCASEPSGAYTGEVSPAMIRSAGAAYVIIGHSERRTY
                     YHEDNAILHRKTWLAIENGLSVIFCCGEVQHEREAGTHFDVVRTQLEEGLFSLPEDAF
                     RKVVIAYEPVWAIGTGLTATPEQAQEMHLHIRTLVAGRYGRECADDMTILYGGSCKPS
                     NAREIFSKPDVDGGLIGGASLKKEDFTAIVESI"
     gene            1062..5570
                     /locus_tag="PK288_08750"
     CDS             1062..5570
                     /locus_tag="PK288_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19053.1"
                     /translation="MNKKLLTLILASLMLPAALMPQVKPAFSGDPARFREELVQFMGE
                     NLSPEHNAILSTFLAKWDSAAFSKENMTRILDLSTQLTGRQIRAVPQFSQFLKTLTDF
                     TNRNRDDAFLGYWLTGFSELLFNPRFSNESITRYIKNTSSLLEENLIFSSGSVKWKVK
                     GAELRFIHDTIFHIDIRNATLTCYSQRDSTEIYNASGTYYPDLQVFRGSKGTVTWEKA
                     GFAAEDVFAEIHNYTINTTRNSFTCDSARLTHKTYFREPVYGILTDQAASVSNKERSI
                     FPQFETYTSQFVLDNIYEGVSYEGGLAFEGAQTRGKGNRSSPAKITLSRNDTLFIKIT
                     ANEFTFTRVGLTSAGTESTLYLGKDSIYHSNLGFSYNANDRQVSLFRTNNPVSASPYY
                     DTYHNLDMYFENLSWDMNTSRVLITRAKGASIGQALFESATFFNAAYFMSLLGLDNYH
                     PLTRLKKFSEWYYSDRFPVSEFAKWLNISPDAVTGLCIDMAAKGFVFYDRDNDEVVIK
                     KKVRDFLDSYAGKKDYDVLTVRSEVRAPMDNAVLDLKTFDMRVMGVRSVFLSDSQRVA
                     IYPYNRELVITRNRGLTFDGVVQAGLFTIYGHDFRFSYDTFKIRLASIDSIRIAVETG
                     KTDPFGNIIVRDVGNILQLTKGELYIDDPANKSGRKRLTQYPIINAMVPSYIYYDKIP
                     GLEDVYRKEDFYFRIDPFTFENIDHFRNEDISLSGEFVAGNILKPMRQQMTVQENNSL
                     GFEMNIPPEGVEVYDGRGKIFDHISMSNSGLIGRGTLRHLTSVTRSDEYRFFPDSMLA
                     RATVFNIENDGSGLFPELNSEDVKIKWLPGRDEWLAENAKEKQFNMFANGTILDGKLK
                     LMPSGLSGSGEINMTDSKIVSNGFSFTSSQIRADTADYSFKSPSTSGYAFIAENANTE
                     IDFSQQKAAFHLNTGSSFVRFPEIEYICTMTDFSYDMGTKILAMEQRGKSGSGPGLIP
                     PEKLLALDMRTLEKPTFIAVNSLRDTISFAARKASYNVEKEYIEAGDINYIHIADALI
                     QPENGSITINRKANISKLSNAFIAVNNRHLLHSANITIESARKYSGSAIYNYVDDSRK
                     IHPITFGEITVDTLTTSGKGFIPENQNFMLSQAFTFSGDVSLSARRNNLFFTGSAGIV
                     NSCEKIRSYTIKFRSEIDPGNVMIPIGEKPRDKNDNLVFSGSFLNIDSTHIYPAFLSM
                     QKSWSDVGLVTASGVLYYNKSLGRYQIASVEKIADPGRYGNLVSLDRNSCILSGEGRL
                     NFGASLDLVKMNTAGTIRHNTDSGKVNIRTIMALSFYFSPAALKMMADDIKLVPSLKT
                     VNLNSEFNNKGMKDLYGESLANQLRDEVSLFGTFRNMPREFDVQLLLNDVSLYWNESS
                     SSFRSTGRIGIGFIGDQALNVYVDGFIEIQRRRSGDLIDIYLKANPSTWYYFSYFRGV
                     MMAQAGNLEFNRLLSSIKLKDRRHPQATNRVPYTYMIAVEDRLSRFLRRMSDEPDEGS
                     SPPEGQIW"
     gene            5592..6296
                     /locus_tag="PK288_08755"
     CDS             5592..6296
                     /locus_tag="PK288_08755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPM19054.1"
                     /translation="MQIFYAPGIKGDMHYLDEGESRHCLRVLRMRKGDMVRLVDGKGT
                     LYGGCISDTVQGRCGIRITSMTAGYEKRDYRLHIAISPLKNQDRFEWFVEKSVEAGID
                     VITPIICKNTEKRGIKEDRINNIIISAMKQSVKAFRPLLGKPVPFGEFIRRDHEGTRM
                     IAHCSGEFERNAIGKCIRRGENALILIGPEGDFSREEISAALANGFRSVELGKSRFRT
                     ETAGVAACLAVYFINQ"
     gene            complement(6783..7370)
                     /locus_tag="PK288_08760"
     CDS             complement(6783..7370)
                     /locus_tag="PK288_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM19055.1"
                     /translation="MKKNMSDYELIERFIKGEQSCFEHLIHRHKNKVFAYISLYIRDQ
                     ALAEDLFQDTFLKVIQSVKSGKYYDNGKFISWVMRIAHNLIIDHFRRVKQLNTVSNDD
                     YESDLFNSKKLAEANVEDHLIKKQIQKDIRKLISQLPDDQREVVILRHYSGLSFKEIA
                     DITDVSINTALGRMRYALINMRKMMEEKKISLTYN"
     gene            7718..>9183
                     /locus_tag="PK288_08765"
     CDS             7718..>9183
                     /locus_tag="PK288_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HPM19056.1"
                     /translation="MDGKIEIKGARVHNLKSINVSIPRNQLVVITGVSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGRMNKPEVDFINGIPPAIAIEQKVNTRNPRSTVGTS
                     TEIYDYIRLLFARIGRTYSPISGREVKRHSADDLVKFIGTLEEGSRVILTSPAVIPEN
                     IRVAEYLSFLVKQGFSRIEENGELTRIDDPGAEKRFHPGQSLNIVIDRIVVNNDPDNL
                     SRTADSAQTAFQTGKGYCQAIIQSDNGFNRAGFSTKFEEDGIEFEEPSEFLFSFNNPV
                     GACPLCEGYGKVIGIDENLVIPDPNLSVYQDAIVCWKGESMKKWKERLISNAPLFGFP
                     VHKPYYQLSQEQKDLLWNGNRYFYGINRFFEHLEEKKYKIQYRVLLARYRGKTVCPEC
                     RGTRLRKEASYVRVAGITVQELVSMPVGRLYDFFLNIILDEQEAKITERLLREIRQRL
                     KFLIDTGLSYLTLNRLSSTLSGGESQRINLAASLGSNLT"
BASE COUNT         2556 a         2152 c         2319 g         2156 t
ORIGIN      
        1 cttcattgcc gatgctcctg ccggatatta cagggatctt gatattaaag cataccggat
       61 tgccgttata cttaccggat tgtcagtgat catcctgatg ctgcttcccg ctgtggtggg
      121 gaagaaagac acgcggctgc acggagatca ggggattgca taggactatt gctgcaggct
      181 atacaggtaa ggaggtatgt tcccatctta tgtgtgaaac agataacagt atggatccgg
      241 gtgactgcat ggaaatgtca cacggttgaa taacagaata aatcaaaaca aaaattaaag
      301 atctatgaga aaaaaaattg ttgccggcaa ctggaaaatg aacatggagc tggctgaagg
      361 actgaaattt gccagtgaac tggatgcgta cttcaaagaa aagccggcag cggccggggt
      421 tgtgctgtgc acccctttca ttcatcttgc cggggcggct gatattttaa agggcgggaa
      481 tgtggcgctg ggtgcccaga attgtgcatc ggagccgtca ggcgcataca ccggcgaggt
      541 atccccggct atgatacgca gcgccggtgc agcttacgtc attataggtc attcggaaag
      601 aaggacctat taccacgaag acaacgccat cctgcacagg aagacatggc ttgcaattga
      661 aaacggcctc agtgtgattt tctgctgcgg cgaggttcag catgaaaggg aagccggcac
      721 tcattttgat gtggtcagaa cacagcttga ggagggattg ttcagcctgc cggaagatgc
      781 cttcaggaaa gtggtcattg catatgaacc cgtatgggca attgggacgg ggctcacggc
      841 cacgcccgaa caggcgcagg agatgcacct gcatataagg acactggttg cgggcagata
      901 tggcagggaa tgcgccgacg atatgaccat actctacggc ggcagctgca agccttcaaa
      961 tgctcgcgag atcttctcaa aaccggatgt ggacggagga ctgattggag gcgcttcact
     1021 gaaaaaagaa gatttcactg caatagttga atcgatataa aatgaacaag aagcttctta
     1081 ccctgatact ggcatcgtta atgctgccgg ccgctctgat gccgcaggtt aagcctgcat
     1141 tcagcggcga cccggcacga ttcagggaag agcttgtcca attcatgggc gaaaacctgt
     1201 cccctgaaca caatgccatc ctcagcactt ttcttgccaa atgggacagc gcagctttca
     1261 gcaaggaaaa catgacgcgc atacttgacc tctccaccca gctcaccggg cgccagataa
     1321 gggctgtgcc gcagttcagc cagttcctga aaacccttac cgactttacc aaccgaaaca
     1381 gggatgatgc attccttggc tactggctta caggcttcag tgaacttctt ttcaatcccc
     1441 ggttctcgaa tgagagtatt accaggtata taaaaaatac aagctcactg ctcgaggaga
     1501 accttatctt ctcatcaggc tcggtaaaat ggaaagtgaa gggagcggaa ctcagattca
     1561 tccacgatac catattccat atagatatcc ggaacgccac cctgacctgc tactcccagc
     1621 gcgacagcac cgagatatac aatgccagcg gaacctatta tcccgacctg caggtcttcc
     1681 gcggatcgaa aggtacggtg acatgggaga aagccggatt tgccgcagaa gatgtctttg
     1741 cagagataca taactataca ataaacacaa cacgcaattc ctttacctgc gattcggcac
     1801 ggctcactca caaaacctat ttcagggagc cggtatacgg tatcctcacc gaccaggcag
     1861 ccagtgtctc aaacaaggaa agatcgatat tcccgcagtt tgaaacctat acaagccagt
     1921 tcgtcctgga caacatatat gaaggggtta gttacgaggg cggcctggca ttcgagggag
     1981 cacaaaccag gggcaaggga aacaggtcga gccctgcaaa aatcacgctg tcaaggaatg
     2041 acaccctttt tataaagatc actgccaacg agttcacctt cacaagggtc ggtctcacct
     2101 cggcaggtac cgaatcgacc ctgtacctgg gaaaagattc catttatcat tcaaacctgg
     2161 gcttttccta caatgccaat gacaggcagg tgagcctctt ccggaccaat aatccggttt
     2221 cggcaagccc ttactacgat acctatcata atctagatat gtatttcgag aacctgtcgt
     2281 gggacatgaa cacttcgcgg gtactgataa ccagggctaa aggagcttca ataggacagg
     2341 cattgttcga atcggcaaca ttcttcaatg ccgcctactt catgagccta ctgggccttg
     2401 acaactacca tcccctgacc aggctcaaga aattcagcga atggtattat tccgacaggt
     2461 tcccggtgag cgaatttgcc aaatggctga atatctcgcc cgatgcagtg acgggattat
     2521 gcatcgacat ggcggcaaag gggtttgtat tctacgacag ggataatgat gaagtggtga
     2581 tcaagaagaa agtaagggac ttcctcgatt catatgccgg aaaaaaagac tatgatgtgc
     2641 tgacggtgag aagcgaagta agggccccga tggacaatgc cgtcctcgac cttaaaacat
     2701 tcgacatgag ggtgatggga gtacggagcg tattcctgag cgattcgcag cgcgttgcaa
     2761 tttacccgta caaccgcgaa cttgttatta caagaaaccg gggacttaca ttcgacgggg
     2821 tggtgcaggc aggtttgttt acaatttacg ggcatgattt cagattcagc tacgacactt
     2881 ttaaaatacg ccttgcaagc atcgactcaa tacgcatcgc agttgaaacg ggaaaaacgg
     2941 atccattcgg caacattatc gtcagggatg tgggaaacat tctgcagctc accaagggag
     3001 agctgtatat tgacgatccg gcaaacaagt cgggacgaaa gcggctcaca cagtatccga
     3061 taatcaacgc gatggtgccc tcatacatat attatgacaa gatcccggga ctggaagatg
     3121 tttaccgtaa agaagacttt tatttcagga ttgatccgtt cacgttcgag aacatcgatc
     3181 atttcagaaa tgaagacata agcctgagcg gggagttcgt agcgggtaac atactgaagc
     3241 ccatgcgtca gcagatgacg gtgcaggaaa acaactccct gggctttgaa atgaatatac
     3301 cacctgaagg tgttgaggta tacgacggca ggggaaagat ctttgatcac atcagcatga
     3361 gcaacagcgg gctcataggc agggggacac tgaggcacct gacctcggtc acacggtcgg
     3421 atgagtacag gttctttccc gactcaatgc tggccagggc cacggtcttc aatattgaaa
     3481 atgacggatc ggggctgttt cccgaactga acagtgagga tgtgaagata aaatggcttc
     3541 cgggcaggga tgaatggctt gctgagaatg caaaggaaaa gcagttcaac atgtttgcca
     3601 acggaacaat tctcgacggt aagctgaaac tgatgccctc ggggctcagc ggctccggtg
     3661 aaataaacat gaccgattcg aaaatcgtgt caaacgggtt ttccttcaca tcatcgcaga
     3721 taagggccga caccgccgat tacagtttca agtcgccatc aacgagcgga tatgcgttca
     3781 tcgcggagaa tgcaaacacc gagatcgatt tcagccagca gaaggcagcg ttccatctca
     3841 atacaggttc gtcctttgtg aggttccccg aaatagaata catctgtacc atgaccgact
     3901 tcagctacga tatgggaacg aagatcctgg ccatggagca gagaggaaaa tcaggatcgg
     3961 ggccgggact gattcctccc gagaagctgc tggcacttga catgcgaacc ctggaaaaac
     4021 caacctttat tgccgtcaac agcctgaggg acactatatc attcgcagcc cgcaaggcca
     4081 gctacaatgt tgaaaaggaa tatattgagg ccggagatat caattacatc catatagcag
     4141 atgccctcat ccagcccgag aacggaagca tcacaatcaa caggaaagca aatataagca
     4201 agctgagcaa cgccttcatt gctgtaaaca acaggcacct cctgcattct gccaacataa
     4261 ctatcgaatc ggccaggaaa tattcaggca gtgcgattta taattatgtg gatgacagca
     4321 ggaaaatcca tccaattact ttcggtgaaa taaccgtcga cacgcttaca acctccggaa
     4381 agggattcat tcccgaaaac cagaacttca tgctcagcca ggcctttacc ttttccggcg
     4441 acgtgagcct gtcggcacgg aggaacaacc ttttcttcac cggttcggcg ggaatagtca
     4501 acagctgtga aaaaatcagg agttatacca taaaattcag atctgaaatt gatccgggga
     4561 atgttatgat tcccatcggt gaaaagccgc gggacaagaa tgacaatctt gtcttttcgg
     4621 gatcattcct taatattgat tccacacata tctaccctgc gttcctttcg atgcagaaat
     4681 cttggtccga tgtcggactg gttacagcct caggcgtcct gtattacaat aaatcgctcg
     4741 gaagatacca gatagcatcc gtggaaaaga ttgctgatcc gggcaggtac ggcaaccttg
     4801 tttcgctcga caggaattca tgcatcctgt cgggggaagg caggcttaat tttggtgcca
     4861 gcctcgatct cgtaaaaatg aacacggcag gaaccatcag acataacacc gattcgggca
     4921 aagtcaacat acggaccatc atggcgctca gcttctattt ctcccctgcc gccctgaaaa
     4981 tgatggccga tgacattaag ctcgtgccat cactgaaaac agtcaacctc aattcagaat
     5041 tcaacaacaa gggcatgaag gacctttatg gcgaaagcct ggccaaccag ctcagggatg
     5101 aggtcagcct gttcgggaca ttccgcaaca tgccaaggga attcgatgtc cagctgcttc
     5161 tgaatgatgt cagtctctac tggaacgaat cttcatcatc attcaggtcg acgggcagga
     5221 taggcatcgg atttatcggc gatcaggccc tgaatgtgta tgtcgacggt ttcattgaga
     5281 tacagagaag gcggtccggc gatctgatcg atatttatct caaggcaaac ccgtcgacat
     5341 ggtattattt ttcctatttc aggggagtga tgatggctca ggccggaaat cttgaattca
     5401 acaggctcct tagttcgata aagctgaagg acagaaggca tccccaggca accaacaggg
     5461 ttccgtatac ctacatgata gctgttgagg accgcctcag ccgcttcctg agaagaatgt
     5521 cggacgagcc cgatgaaggg tcgtcaccgc ccgaaggaca gatctggtga caaacctgaa
     5581 cccagtacag aatgcagatc ttctacgcac ccgggattaa aggcgatatg cactaccttg
     5641 acgaaggcga atcacggcat tgcctgaggg tactgcgcat gcggaagggt gacatggtaa
     5701 ggctggtcga cgggaaagga accctttacg ggggctgcat cagcgatact gttcagggtc
     5761 gctgcgggat aaggataacc agcatgaccg ccggatatga aaaaagggat tacaggctcc
     5821 atattgccat ctcccctctc aagaaccagg atcggttcga gtggttcgtt gagaaaagcg
     5881 ttgaagccgg aatagacgtg ataaccccga ttatctgcaa aaacaccgaa aaacgaggaa
     5941 taaaggaaga caggatcaac aatatcatca tttcagcaat gaagcagtcg gttaaggcct
     6001 tcaggcccct gctcggcaaa ccggtgccgt tcggggaatt catacggcgg gatcatgaag
     6061 gcacacgaat gattgcgcat tgctccggcg aatttgaaag aaatgccatc ggcaaatgca
     6121 tccgcagggg ggagaatgca ttgatcctga taggtccgga aggtgatttc agccgggaag
     6181 agatatctgc agcccttgca aacggcttca ggtccgttga actgggaaaa agcaggttca
     6241 gaactgaaac agccggtgtg gctgcctgcc ttgcagttta tttcataaac cagtagcacc
     6301 cgtgctgccc gcaacaggga agcctggcca gcaggaaata tttcgcggtt ttcagcaaag
     6361 gaacaggata atcccagatc ccggacggac aaaataatcc ggcggtcgcg cggacattat
     6421 gttctgatta gtaaagtttt gccggtttcc gatgcaaacc cgccactgtt aaggcagggt
     6481 ataatataaa agccgtcgcg tttaatttgc gcgacggctt tcggtttctc actgttgctc
     6541 cctgacttta gcaatcagct ttcgtgtcag acgcttcccc gcaacaggtt tcttgacctg
     6601 ttttagaagt ttgatcacat cattaaattc cgggtaaaat ccccatgcat catcgtagat
     6661 gtcagttttt tgtactaaaa tttcctgata tgaaagaagt cgggtagaat ttaatcccat
     6721 aggccgttta ttttggtttt catggaaaac ggcccatggg atttattatt gtttaaaaat
     6781 tgttaattat aggtaagtga tatttttttc tcttccatca tctttctcat gttgatgagt
     6841 gcatatctca tccttccgag cgcagtattg atgctaacgt cggtaatgtc agctatttcc
     6901 ttgaagctga ggcccgaata gtgccgcagg ataacaacct cacgctggtc gtcgggcagc
     6961 tggctgatca gtttccggat atctttctgg atctgctttt taatcagatg atcctcaaca
     7021 ttggcctcag ccagtttttt ggagttgaac aggtcggatt cataatcgtc gttcgacacg
     7081 gtattaagct gctttacgcg cctgaaatga tcgattataa ggttatgggc gatgcgcata
     7141 acccaggaaa taaatttccc gttgtcgtaa tatttgcctg atttaaccga ctggatcact
     7201 ttcaggaatg tgtcctggaa aagatcctcg gccaatgcct gatcgcggat ataaagactt
     7261 atatacgcaa atactttgtt cttgtgacgg tggattaggt gttcgaagca ggactgctca
     7321 ccttttatga acctttcgat cagttcataa tcggacatgt tttttttcat agctctctct
     7381 gattttaaaa aaaattagag tagagttgtc ctataggcag tcgatttagt tggttaacgg
     7441 tacaaagaaa atgatttttt ttaaattcca aatgtttttt gggatttttt tgctgaaaaa
     7501 acaaaaaaaa tgtccgttca tgtaaaattt agatcctttc gtcaaagaaa ttccggtttt
     7561 tgtcaaagaa aatatgaagg tttcacttaa cggcggatgt tatccgggga atttgccccg
     7621 gacggcagca atgataataa aagaagtcag taagtaccgc agaaattatt tttgaaaccc
     7681 ttacctttgt ggccttgatt aaagcaagtg gtcagcaatg gacgggaaga ttgagataaa
     7741 gggtgctagg gttcacaatc tgaaaagtat aaatgtatca ataccacgca accagctggt
     7801 tgtgatcacg ggtgtgtcgg gatccggaaa atcctccctg gccttcgaca ctatctatgc
     7861 tgaagggcag cgaagatatg ttgagagcct gtcgtcgtat gcaaggcagt tcctgggaag
     7921 gatgaacaag ccggaggtgg attttataaa tggtattcct ccggccatcg ccatagaaca
     7981 gaaggtaaac acgcgcaatc cccgctccac cgtcggaacc tcaaccgaga tctatgacta
     8041 cataaggctt ctctttgcca ggatcggccg cacatattcc cccatatcgg gcagggaggt
     8101 aaaacgtcac agcgccgatg atcttgtgaa attcatcggg acacttgagg agggatccag
     8161 ggtgatcctg acatctcccg ccgttatccc cgaaaacata agggttgcgg aatacctctc
     8221 ctttcttgtc aaacagggat tcagccggat tgaggagaac ggagaactga caaggatcga
     8281 cgatcccggg gctgaaaaga gattccatcc cggacagtca ctgaatatcg ttatcgacag
     8341 gatagtggtg aacaatgatc ctgacaacct cagccgcaca gccgactccg cacagaccgc
     8401 cttccagacc gggaaaggtt actgccaggc aatcatccag tcggacaatg gattcaacag
     8461 ggcgggtttc tcgacgaaat ttgaagaaga cggtattgag ttcgaggaac cgtcggagtt
     8521 tctattcagt ttcaacaatc ctgtcggcgc ctgtccgctg tgcgagggct atggcaaggt
     8581 aatcggaata gatgagaacc tggtgatacc cgatccgaat ctttcggtgt accaggacgc
     8641 gattgtatgc tggaagggtg aaagcatgaa gaaatggaag gagcggctga taagcaatgc
     8701 accccttttc ggttttcccg tgcacaagcc gtattaccag ctgtcgcagg agcagaaaga
     8761 cctgctgtgg aacggcaaca ggtatttcta tggtataaac aggttctttg aacatcttga
     8821 agagaagaag tacaagatac agtaccgggt tcttctggcc cgttaccgcg gcaagacagt
     8881 atgtccggaa tgcaggggaa cgaggctcag aaaggaggca tcatatgtca gggttgcagg
     8941 catcaccgta caggaactgg tttcaatgcc cgtgggcagg ctttatgatt ttttcctgaa
     9001 catcattctc gatgagcagg aggcaaagat cactgaaagg cttctcaggg agatacgcca
     9061 gcgcctgaag ttcctcattg acaccgggct ctcctatctc accctcaaca ggctgtcatc
     9121 aaccctgtcg ggcggtgaat cgcagaggat aaatctcgcc gcttcgcttg gaagcaacct
     9181 gac
//
LOCUS       DAOOYG010000102         9055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_8428_length_9055_cov_8.363444, whole genome shotgun sequence.
ACCESSION   DAOOYG010000102 DAOOYG010000000
VERSION     DAOOYG010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8428_length_9055_cov_8.363444"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(605..3412)
                     /locus_tag="PK288_08770"
     CDS             complement(605..3412)
                     /locus_tag="PK288_08770"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPM19057.1"
                     /translation="MKRIINLFILLAAGIMFSCTAKKNESPFSVPFEKYKLSNGLTVI
                     LHEDKSDPIAAVAVVFHVGSSREVPGKTGFAHLFEHMMFQRSENVGEDQLFRLIQGAG
                     GELNGGTSFDYTVYYEVIPKNALEMALWLESDRLGYLENTVTQTAFANQQNVVQNEKR
                     QGVDNTPYGHNEALILKNIYPQGHPYSWDVIGEMEDLQNATVEDVKAFHRKFYGPNNA
                     TLVVAGDINKEEVKAMIEKYFGEIPAGQPVEKTSPRPATLSSTIKLYHEDNFAKAPML
                     TMVFPTVEQFSKDSYALDYLGQLLSSGKKAPLYKVLVRDKKLTSSVYAYNQSLELAGT
                     FEISVTANPGVNLTDVEKAVFEAFDLFEKEGFSEADLTALKAKNETSFYGQFSSILSK
                     SFTLGEYQMFRDDPAYYNNDFKAEQAVTSQDIKNVFEKYIKGKNFVMTSFVPKGEVNL
                     IAEGSKNAEIVEEDVTRAAQVETTAEVEEPVVKTPTRIDRSVQPPLGPDPVVTLPEVW
                     KGSLSNGMTLYGIRQSELPLVQYSVIIDGGHLLDPVEKAGIASMTAALMNEGTKNKTP
                     EELEDAIRLLGASISFTGGPENISIRVSTLSRHFEKTLDLVGEMLSEPRWDTVQFELA
                     KSRVVNNLKRNKANPSYLASNTLNSLIFGKGNILAIETAGNEQSVNSLTINDLKDFYS
                     RNFSPSNAKFIVAGDIDQERVIRALEKLSAGWQPKKVTIPPVTVPDAPGKSQIFFVDV
                     PGAKQSVIYIGCPSLPRTNPDYYPAYVANYKLGGSFNGIFNLILREEKGFTYGARSYF
                     NGAKNYGTFTATSMVRTNSTLESVEIFKTEMEKYRREIPQDYVDFTKSALLKGNARNF
                     ETLWNLLSMLNNIAVYDLPVNYIKQEEAFVQGLTAEKILELARKYIDPSRMYYVVAGD
                     AATQLKGLEKVGLGKPVLVKD"
     gene            complement(3584..3991)
                     /locus_tag="PK288_08775"
     CDS             complement(3584..3991)
                     /locus_tag="PK288_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19058.1"
                     /translation="MLQMGIFLLSLSFDPPKESDQRKGGRKRQPPLFFAICAEAISRS
                     KKHGAVRAFSGLPAHRRIQYAGIATGICFSIKLLAALEITYYAPSGLIVTGDPFPGCR
                     GGQVTKEKEPVRRCAQNIDIRMAFTTARSGPGA"
     assembly_gap    4427..4526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4568..4640)
                     /locus_tag="PK288_08780"
     tRNA            complement(4568..4640)
                     /locus_tag="PK288_08780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4605..4607),aa:Gly,seq:gcc)
     gene            complement(4725..5363)
                     /locus_tag="PK288_08785"
     CDS             complement(4725..5363)
                     /locus_tag="PK288_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19059.1"
                     /translation="MKRLAFSVFAFLTCVAAYPQEKNTEIPQEFVKNKDAAQDAPVTA
                     TIRSATRLFSDRNDLTSVIMVIPKDSTVVVLGSVEPFLNVTFAGMEGYIYARHAEVHK
                     PVAAAEPPLPSPSQRTSDDRDIPSTRPVQQRQKASRYEYLQHKYGAALASELYAGKIW
                     KGMTGQMIRDSWGSPIKINMVISGNNTREEWIYRNTWLYLRNGELLEWGPVR"
     gene            complement(5724..6419)
                     /gene="ric"
                     /locus_tag="PK288_08790"
     CDS             complement(5724..6419)
                     /gene="ric"
                     /locus_tag="PK288_08790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HPM19060.1"
                     /translation="MEKNNYNNRTLGEIVTEDFRTAGIFQNAGLDFCCGGKKPLEQAC
                     REKNISFEEIVRKLEDVKTAPQNPGQNFNEWKPDFLADYIVNIHHSYVRKNLPELDFY
                     TKKIADVHGDRHPELIEVADLFGRISAELKQHLQAEEEVLFPAIKEAMKGNSPKVRET
                     ISSEIARMSGEHDFVGGAMDKINEITNGYEVPADGCNTYMVAFRLLNQFEDDLHIHVH
                     LENNILFPGALTL"
     gene            complement(6550..6750)
                     /locus_tag="PK288_08795"
     CDS             complement(6550..6750)
                     /locus_tag="PK288_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPM19061.1"
                     /translation="MKPAAKKEFFVYALVSLTDGVIYVGIATDCEARLKQHNSGKSKY
                     TSGHRPWKLFYKEFAGESFYPL"
     gene            complement(6871..6943)
                     /locus_tag="PK288_08800"
     tRNA            complement(6871..6943)
                     /locus_tag="PK288_08800"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6908..6910),aa:Phe,seq:gaa)
     gene            7060..8280
                     /locus_tag="PK288_08805"
     CDS             7060..8280
                     /locus_tag="PK288_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPM19062.1"
                     /translation="MELLYHTLGNGIRLVHHPVTGLVAHCGLIIGTGSRDEEEHEHGL
                     AHFSEHMLFKGTAKRKAYHILSRLEDVGGELNAYTTKEETVVHASFLKDDYERAIEII
                     SDMVFNSTYPEKEIEKEKDVIIDEINSYLDNPAELIFDDFEELIFAGRSIGHSILGTP
                     STVKCFTAGSIRDFISRNYNTSQMVFCSVGDISYARLTSLFRKYFEHIPEMKGRQCFS
                     PPWSYKPACLTKNLDTYQDHCIIGNVAYNLRDERRPGMFLLNNILGGQGMNSRLNLSL
                     REKNGLAYNVESSYIPYCDTGAFSIYFGTEDQNLERSIMAARAELDKLRTTRLGIVQL
                     GKARNQIKGYLARGFENHESLMLSLGKSLLVFDTVEDPGEIYRKIDNISSSHLLETAN
                     DIFDPDMLSTLIYR"
     gene            8283..8933
                     /locus_tag="PK288_08810"
     CDS             8283..8933
                     /locus_tag="PK288_08810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPM19063.1"
                     /translation="MQKFPDNFLLELHTPVDPVLDDLYRQTHIKFVNPNRICGHLQGR
                     LLEFISGMKASHNILEIGTFTGYATICLARGLRLGGRIITIEPNDELLAFSNSYFRKA
                     GVAERVIQITGRAQDTVPGLDMKFDLIYIDGDKREYAEYFNLAINKLATGGLILVDNV
                     LWGGKVLEKDKRDPQTRGIIEFNEMIKKQTGIENVILPAWDGLMIIRKKGARTGHL"
BASE COUNT         2284 a         2154 c         2053 g         2464 t
ORIGIN      
        1 aaacacccac cgggtgtttt cttaacgctc gcccctacca tccgggcatt aattatctca
       61 ggtggataaa tcaactattt aacaaaacaa taaaaaaccc tgtcaatttt taccgggatg
      121 gtggaatcgg ccggcgctgc agtctggctg cttcgctgaa aacacccacc gggtgttttc
      181 ttaacgctcg cccctaccat ccgggcatta attagcattt ggtggctata ttaaatattt
      241 taaatatcat taaagaaccc agccatcttc acggggatgg tggaatcggc cggcgctgca
      301 gcctggctgc ttcgctgaaa acacctaccg ggtgttttct taacgctcgc ccctaccatc
      361 cgggcattaa ttatctcagg tggataaatc aactatttaa caaaacaata aagaaccctg
      421 tcaattttta ccgggatggt ggaatcgtcc ggcgctgcag tctggctgct tcgctgcaaa
      481 cacccacccc tgccatccgt ttcagcctgc tgaaatttgg ttcagccgtg taaaattaag
      541 taaataatct ttaaataaaa cacaaaaaac cctctcagcc gggagagggt tttgatccgg
      601 cgcattaatc cttcacaagc acaggcttgc ccagcccgac cttctcaagt cctttaagct
      661 gggttgcggc atcgcctgca acaacataat acattctgga aggatcgata tatttcctgg
      721 cgagttcgag gatcttttcc gccgtcaatc cctggacgaa tgcttcctcc tgttttatat
      781 aattgaccgg cagatcatag acggcaatat tgttcagcat ggacagcagg ttccacaggg
      841 tttcaaaatt cctggcattg cctttcagaa gtgctgattt agtgaaatcg acgtaatcct
      901 gcggtatttc cctgcggtac ttctccattt cggtcttgaa aatctcgacg gattcaaggg
      961 tggagtttgt acggaccatc gatgtggctg taaaggtacc gtaattcttc gccccgttga
     1021 aataagatct ggcaccgtag gtaaaacctt tttcctcgcg cagtatcagg ttgaagatcc
     1081 cgttaaatga tccgccaagc ttgtagttgg caacataggc cggataataa tccggatttg
     1141 tccgtgggag ggaggggcag ccaatgtaaa taaccgattg cttggctccc ggaacgtcaa
     1201 caaaaaatat ctgtgatttt cccggtgcat cgggaaccgt tacgggagga atagtcactt
     1261 ttttcggctg ccatccggcg ctaagttttt ccagtgccct tatcactcgt tcctggtcaa
     1321 tatcgccggc tacaatgaat tttgcattcg aaggggagaa attcctgctg tagaaatcct
     1381 tcaggtcgtt tattgttaat gaattaacag attgttcgtt acctgcggtc tcgattgcca
     1441 ggatattgcc tttgccgaat atcaggctgt taagcgtgtt tgatgcaagg taggatgggt
     1501 tagccttatt tctcttcaga ttgttaacga ccctgctttt tgcaagttca aactgaacag
     1561 tatcccagcg cggctcggaa agcatctccc ccacaagatc cagggttttc tcaaagtgcc
     1621 tggaaagggt gctcactctt attgaaatat tctcagggcc cccggtgaac gaaatgcttg
     1681 ctcccagaag ccttattgca tcctcgagct cttcgggtgt tttatttttt gttccttcat
     1741 tcataagtgc cgcggtcatg gatgcaattc cggctttttc aaccggatcg agaagatgtc
     1801 cgccatcgat gatgactgaa tactggacaa gcggcagttc gctctgcctg attccgtaaa
     1861 gtgtcattcc gttggaaaga gatcctttcc agacttcggg aagagtaacg accgggtcgg
     1921 gaccgagggg gggctgaacg gaacggtcta tcctggtcgg cgtcttcaca accggttcct
     1981 caacttcggc tgttgtttcc acctgtgctg cccttgtcac atcttcttcc actatttcag
     2041 cattttttga tccttctgca ataaggttca cttcaccctt gggaacgaag cttgtcatca
     2101 cgaaattctt ccctttaata tatttctcaa aaacattttt gatatcctgt gacgtgactg
     2161 cctgttcggc tttaaaatcg ttgttgtagt atgcgggatc gtccctgaac atctggtatt
     2221 ctcccagggt gaaagattta ctgagtatac tgctgaactg gccgtaaaag ctggtttcat
     2281 tttttgcctt taaggcagta aggtccgctt cagaaaaacc ttccttttca aacaagtcaa
     2341 atgcttcaaa aacagctttc tcaacatcgg tgagattcac acccgggttt gctgtgacag
     2401 atatctcaaa agtccctgcg agttcaagcg actgattata ggcatagact gacgaggtta
     2461 gctttttgtc cctgacaagg accttgtaca gtggggcctt tttgccgctg gacagaagct
     2521 ggccgagata gtcgagcgca tatgaatctt ttgagaattg ttcaacggtc gggaacacca
     2581 ttgtcagcat gggagctttg gcaaaattgt cttcgtggta gagttttatt gttgaagaca
     2641 gtgttgcagg cctgggagaa gttttctcaa caggttggcc ggcgggaatt tctccgaaat
     2701 acttttcaat catggctttt acctcctcct tatttatgtc gcccgctaca acaagcgttg
     2761 cattgttcgg gccatagaac ttccggtgga aggccttgac atcctcaacc gtcgcattct
     2821 ggagatcttc catctccccg atgacgtccc agctgtacgg atgaccctgt ggatatatgt
     2881 ttttcaatat aagggcttcg ttgtgcccgt atggggtgtt gtcaactccc tggcgttttt
     2941 cattctgaac aacattctgc tggttggcaa aagctgtctg ggttacagta ttctcgagat
     3001 atcccagtct gtctgattca agccacaggg ccatttcaag ggcattcttc ggaattacct
     3061 cataataaac cgtgtagtca aaactggtcc ccccgttaag ttctcctcct gcaccctgta
     3121 taagcctgaa cagctgatct tccccgacat tttccgacct ctggaacatc atgtgctcaa
     3181 agaggtgggc aaatccggtc ttgccgggaa cctcacggct tgatccaaca tgaaagacca
     3241 cggcaacggc tgcgatcgga tctgatttgt cttcgtggag gatgacagtc agtccgttgc
     3301 tgagtttgta cttttcaaac ggtactgaaa aaggtgattc gttctttttt gccgtgcagg
     3361 aaaacataat tcctgcagcc agaagaataa ataaattgat gattcttttc attgacggga
     3421 aattgtagtt tgaaaactga acatgtattt ggagtgctaa attacgaaaa atccggggag
     3481 cctgtcattc acacagaatt ttctacggta ttttctcaat gtgtttttat gcctttctag
     3541 aatattggct tagcagaaga aataagaaaa ttcatgttcg gagttaagct ccgggacctg
     3601 accgggctgt cgtgaaagcc atccggatat caatattctg agcacaacgg cgcacaggct
     3661 ccttttcttt ggtcacttgc cccccgcgac agccggggaa aggatcacca gtgacaataa
     3721 gccctgaagg ggcgtaatat gtaatttcaa gagccgccaa cagtttaatg gaaaaacaaa
     3781 tacccgtcgc aataccagca tactgaatcc ggcggtgcgc gggcagaccg gaaaaggcgc
     3841 gaaccgctcc atgttttttg gagcgggaaa tggcctcagc gcaaatggcg aaaaacaggg
     3901 gcggttgtct ttttcggcct ccttttcttt ggtcactttc ttttggagga tcaaaagaaa
     3961 gtgacaataa aaaaataccc atctgcaaca tcttcttctt tttaattgcc tcatccctcc
     4021 ttcaggcata gcctgagtcg gtctggcaag cgacgggatt gtctcgcctg cggctcgacg
     4081 gaccctgttg ggagatgctt tcagacaaaa agggccactg tcgtggcctt cttttttaat
     4141 tgcctcatcc ctccttcagg catagcctga gtcggtctgg caagcgacgg gattgtcttg
     4201 cctgcggctc gacggaccct gttggagatg tcatcaaaca gagtcgccgc tgtcgcgtcg
     4261 ccatttttta tttattcaat actcctccat gtggaacatg tcgtcgtttt cataaataaa
     4321 aaatgccgca ttttcatgcg gctctgtttg agcgggagac gggattgtct cgcctgcggc
     4381 tcgacggacc ctgttggaga tgtcatcaaa cagagtcgcc gctgtcnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnntcat aaataaaaaa tgccgcattt tcatgcggct
     4561 ctgtttgagc gggagacggg actcgaaccc gcgaccctaa ccttggcaag gttatgctct
     4621 accaactgag ctactcccgc agatacgccg gcaaaattaa aaaaaaaatc ataactccaa
     4681 acctaagttc ctaatttgtg aaaagaatga aggcaagggt ggcgtcatct gaccggcccc
     4741 cattccagga gttcgccgtt gcggaggtaa agccatgtgt tccggtaaat ccattcctcc
     4801 ctggtattgt taccgctgat aaccatgttg atcttaattg gcgaccccca gctgtccctg
     4861 atcatctgtc ctgtcatccc tttccatatc ttgccggcat aaagctctga agccaatgcg
     4921 gctccatatt tatgctgaag atattcatat cggcttgcct tttgccgctg ctgaacgggt
     4981 cgcgtggagg gaatatcccg gtcgtctgag gtcctttgcg aaggagaggg gaggggaggc
     5041 tccgctgcgg caactggttt gtgcacttca gcgtgacgcg catagatgta accttccata
     5101 cctgcaaaag taacattcag gaaaggttca accgacccaa gaacaacaac cgttgagtcc
     5161 ttcggaatta ccattataac ggatgtaagg tcgttcctgt cgctgaaaag ccttgtcgcc
     5221 gaccttattg ttgcagtgac tggtgcgtcc tgggccgcat ccttattttt cacgaattcc
     5281 tgcggtatct cagtattttt ttcctgcgga taagctgcga cacatgttaa aaaagcaaaa
     5341 acggaaaatg ccagtctttt catcccttat ctgctgatta tgaattatct gatccatgca
     5401 atatcatcac gggcaaatat catgacatct gatcctgttg cgccatgaaa cttccgctcc
     5461 ctgttctttt caggctaacg aaagcaataa tacatgaatc aaaaagcgta ccagtatgat
     5521 aatgattaac tcacattttg tgatctgccg ggaaaaaagg ctgcgactct taccggactc
     5581 caaccggatt gtggatccca aagcggatgc aaacttgcct aacctgaaaa tccatctgag
     5641 gcagagtgag gttgttacag ccggcagccg gagcggccat actgaaaagc acccggagcc
     5701 ggtaactgca gcagttgttt gtattacagg gtcaatgccc cgggaaaaag tatattattc
     5761 tcgagatgga cgtgtatatg cagatcgtct tcgaactgat tcagcagtcg gaaggcaacc
     5821 atataggtgt tgcagccatc ggcaggcact tcgtaaccat tggttatttc atttatttta
     5881 tccattgcgc cgcctacaaa atcatgctca ccgctcatac gtgctatttc tgaacttatg
     5941 gtttccctca ctttgggtga atttcccttc atggcttcct ttattgcagg gaacagtact
     6001 tcctcctctg cctgaaggtg ctgcttcagt tcagcgctga tcctgccgaa caggtcagcg
     6061 acctcaatca gttcaggatg cctgtcgccg tggacatctg ctatcttttt ggtgtaaaag
     6121 tcaagttcgg gcagattttt cctcacataa ctgtggtgga tgtttacaat atagtcggcc
     6181 aggaaatcag gcttccattc gttgaaattc tggccggggt tttgaggtgc agttttcaca
     6241 tcttcaagct tcctcactat ttcttcaaag ctgatgtttt tctccctgca tgcctgttcg
     6301 agaggtttct tacctccgca gcagaaatca agtccggcat tctgaaatat tccggcggtt
     6361 ctgaaatctt cagtgacgat ttctcccagg gtcctgttat tgtaattatt cttttccatt
     6421 atttatgata atatgaattg cagctgcaag tataagtaat aattcattaa aagacaataa
     6481 agtcttttaa tgatggagaa atattttgta aaacaggaat tcagggaata atgtcagtgg
     6541 tgagactgac tacaagggat aaaagctttc accagcaaat tccttgtaga acagtttcca
     6601 tggacgatgt ccggaggtat attttgattt tccgctgttg tgctgtttga gtctggcctc
     6661 acagtcggtt gctattccga catagatcac cccgtcagta aggctgacca gggcgtaaac
     6721 gaaaaactcc ttttttgcgg ccggcttcat tcagacaata aaaaaagcga tgcttcagtc
     6781 atcgctctca ctcttgctcc cctccacctt ttctcctcac tcactctcac tctcactctt
     6841 gtttcccctc tcactctcac tctttttctg tggtgccacc gggaatcgaa ccagggacac
     6901 acggattttc agtccgttgc tctaccgact gagctatggc acctgctttt cggaatgcaa
     6961 atataaatct caaaacggtt ctaccaaaac tttgaggtgg aaaatttctg tttgcaatca
     7021 tttcagctat gtgataactt tgcatcctgg caaatcgcaa tggagttatt gtaccataca
     7081 ctcggcaatg gtatcaggct ggtccatcac ccggtcaccg ggctggttgc ccattgcgga
     7141 ctgatcatcg gaaccggatc gcgtgacgag gaggaacatg aacacggatt agcccatttc
     7201 agcgaacaca tgctgttcaa gggaacagca aaaagaaaag catatcatat tctcagcagg
     7261 ctcgaggatg ttggcggtga gctcaatgcc tacacaacca aggaagaaac agtggtgcat
     7321 gcttccttcc ttaaggacga ttatgaaagg gccattgaaa taataagcga tatggttttc
     7381 aattcaacct atcccgaaaa agaaatcgag aaagaaaaag atgtgatcat cgacgagatc
     7441 aattcatacc ttgataatcc cgccgagctg attttcgatg attttgaaga acttattttt
     7501 gcaggccggt caattggtca cagcatcctc ggcaccccgt caactgttaa atgtttcaca
     7561 gccggatcaa tccgggattt catttcaagg aactataaca cgagccagat ggtattctgt
     7621 tccgtcggcg acatttcata tgcaaggctt acttccctgt tcaggaaata ttttgaacat
     7681 attcccgaga tgaagggaag acaatgcttt tcacctccat ggtcatataa gccggcatgc
     7741 ctgacgaaaa acctcgatac ataccaggac cactgcataa taggaaatgt agcctacaac
     7801 ctgagggatg aacggcgccc cggcatgttc ctgctcaaca atatcctggg cgggcagggg
     7861 atgaattcac ggctgaacct ttcgctcagg gaaaagaacg ggctcgcata caatgtggaa
     7921 tcaagctata taccatactg tgacaccggg gctttttcca tttattttgg tacggaagat
     7981 cagaatcttg aaagaagcat catggcggcc agggcggagc ttgacaaatt aaggaccacc
     8041 aggctgggaa tcgtccagct cggcaaggcg cggaaccaga tcaagggata ccttgccagg
     8101 ggatttgaaa atcatgaaag ccttatgctc agcctgggca aaagtcttct tgtcttcgat
     8161 acagtcgaag atcccgggga gatatacagg aagatcgaca atatttcatc ttcccatctt
     8221 ctggaaacag ccaatgatat tttcgatcct gatatgctgt cgacgcttat ttacaggtaa
     8281 caatgcagaa atttcccgat aacttcctgc ttgagcttca cactcctgtc gatccggtcc
     8341 tggacgatct ttacaggcag acacatataa aattcgtcaa tcccaacagg atttgcggtc
     8401 atctccaggg ccgcctgctc gaattcattt caggaatgaa ggcttcgcac aatatacttg
     8461 agatcggcac tttcacgggc tatgccacaa tctgccttgc caggggactg agactgggag
     8521 ggcgcatcat taccatcgaa ccgaacgatg aactgcttgc cttcagcaac tcctatttcc
     8581 ggaaggcagg tgttgcagaa cgggtaattc agatcaccgg ccgtgcccag gatactgttc
     8641 cgggacttga catgaaattt gatctcatat atattgacgg tgacaagcgc gaatatgccg
     8701 agtatttcaa cctggcaata aacaagcttg caacgggagg attaatcctg gttgacaatg
     8761 ttctatgggg aggaaaagta cttgaaaaag ataaaagaga ccctcagacc aggggtataa
     8821 tcgaattcaa tgaaatgata aagaagcaaa ccggcatcga aaatgttatc ctgccggcct
     8881 gggacggact tatgattata cgaaaaaaag gtgcccgaac gggacacctc tgatccataa
     8941 tcaaccgaca agcaacgaaa atcctgaaat tagctgatct cgatggttct cgggggcctg
     9001 gtcctggctt cctagaacca tcgagatcag ctaatttcag gattttcgtt gcttg
//
LOCUS       DAOOYG010000103         9035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_8462_length_9035_cov_5.652450, whole genome shotgun sequence.
ACCESSION   DAOOYG010000103 DAOOYG010000000
VERSION     DAOOYG010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8462_length_9035_cov_5.652450"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PK288_08815"
     CDS             <1..207
                     /locus_tag="PK288_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19064.1"
                     /translation="CFVYICYAAFFNWNKYVNHTRILYQIVFDSKNYTYFLNKENQGP
                     LFGYLVGMLSKRGCHPYRMGEYGI"
     gene            569..1675
                     /gene="gcvT"
                     /locus_tag="PK288_08820"
     CDS             569..1675
                     /gene="gcvT"
                     /locus_tag="PK288_08820"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPM19065.1"
                     /translation="MKNTIFTRFHEAAGARMIPFAGFNMPVEFAGINEEHVAVREGVG
                     VFDVSHMGEFWVTGPDALPFLRYIASNDAASLTDGKVQYTCFPNGKGGIVDDFLVYRF
                     NAEKYLLVVNAANIEKDWEWCVSQAGRFGIRPGKDLVNASDEISQLAVQGPLALKAMQ
                     KLTAAPVAEMEWYTFKIIEFAGIKDVIFSTTGYTGAGGCEIYVRNEDGPKLWEAVFDA
                     GREFNIRSVGLGARDTLRLEMGYCLYGNDINDQTSPIEAGLGWITKFSDDRDFVDKAL
                     LLQQKKEGVKRRLKGFVMIDRGIPRQHYEVVNASGERIGEVTSGTMSPMLRQGIGMAY
                     LDEGYWKPDTEIFIRIRNKDLRARVVNPPFYKKD"
     gene            1773..2507
                     /locus_tag="PK288_08825"
     CDS             1773..2507
                     /locus_tag="PK288_08825"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPM19066.1"
                     /translation="MEKRKLETCFSPALFEPELLHDPIVVIIDILRASSSICTAFANG
                     AARIIPVGTVGEARAHKENGFMVAAERDGYVLDFADFGNSPYNFTKEMVEGRTIVYST
                     TNGTGIIKLASSASCIVIGSFLNLSSLVKWLTAKDKNVLIFCAGWKNRFNLEDTICAG
                     AIAERLMSTSLYSTICDSTHAAIDLWLLARNDVMGYIEKAAQRSRLREKGLDDCLEFC
                     LSCDFTDVIPVLRNGVLETVDLSDQA"
     gene            2601..3671
                     /gene="serC"
                     /locus_tag="PK288_08830"
     CDS             2601..3671
                     /gene="serC"
                     /locus_tag="PK288_08830"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPM19067.1"
                     /translation="MKKHNFYAGPSILSQYTIDKTIEGIRDFAGSGLSVLEISHRSKE
                     FIACMNETIALVKELLDVPSGYQVIFLGGGASLQFAMVPMNLMNSKSAYLNTGEWANK
                     ALKEAKLYGEVTEVASSKESNYNYIPKNYVVPSDADYFHVTTNNTIFGTSLKSDPDVP
                     VPLVADMSSDIFSRPVDVSKYSLIYAGAQKNLGPAGVTVVIIKESVLGRVNRAIPTML
                     DYRTHIKAESMFNTPPVFAVYAAQQTLVWLKGLGGVKAMHKKNTEKARMLYDEIDRNK
                     LFVPTIKDEEDRSIMNICFVMADGYKELEKPFSEFAKSRGIVGIEGHRSVGGFRASTY
                     NALPVESITALIDAMKEFESKN"
     gene            3731..4651
                     /locus_tag="PK288_08835"
     CDS             3731..4651
                     /locus_tag="PK288_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM19068.1"
                     /translation="MKVLVATEKPFAEIAARKIKELIDAAGYEAVFLENYKSASEFAS
                     AVSDADALIVRSDIVSAELLDAAKRLKIVVRAGAGYDNLDLEACTRHNVVAMNTPGQN
                     SNAVAELAFEMMLYMARSGFSGKSGTELRGKTIGIHGFGNVGRYVAEISGGFGMTVSA
                     FDPYIAEDVITGYGIRACKSAKELYSECQYISLNIPFNKETGSSVNYSLLSGMPVPAV
                     LVNTARKEIIDEESLLKMFAERPDFRYLSDVAPDCKALIEEKYSGRFLFTAKKMGAQT
                     EEANSNAGIAAINQIVDYFKTGNQKFRLNK"
     gene            4733..5983
                     /locus_tag="PK288_08840"
     CDS             4733..5983
                     /locus_tag="PK288_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPM19069.1"
                     /translation="MAIVKPFRGLRPPSGIVSDLACLPYDVMNTGEARKMAEGKECSL
                     LHITRAEIEFPPETDIHSEEVYERSRQNFALWQKKGWLIQDDQPCYYIYAQTMKGRTQ
                     YGIAGCASVDDYLDGTIRKHELTRPDKEQDRMVHIRVNNANMEPVFIAYPSVSEIDEI
                     VSSIVEKSTPEYDFISDDGIGHHFWVVNKPGVIRRIEELFAQKVPYTYVADGHHRTAA
                     AALVGKEKREQNPGYDGTEEFNFFLAVHFPDNQLGIMDYNRTVKDLNGLTEEEFLQKL
                     NDGFIVTPKGTRAYRPARLHNFSMYLGGMWYSLTAREGTYDDNDPLGVLDVTILTEQV
                     LKPILDIQDLRRSKRIDFVGGIRGLNELAKRVSQGEMKVAFALYPVSMKQLMAIADSG
                     NIMPPKTTWFEPKLRSGLVIHLLD"
     gene            6104..6793
                     /locus_tag="PK288_08845"
     CDS             6104..6793
                     /locus_tag="PK288_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPM19070.1"
                     /translation="MTDIASNIRSVKTALPPGVKLVAVSKAKSPAEILEAYNAGQRCF
                     GENRVMELAGKKDVLPPDIEWHLIGHLQTNKVKYLVPFISMIESVDSFRLLKIIDNEA
                     SKVNRVISCLLQIHIAEEETKFGFTMDELYAMLENADLYKLRNVNICGVMGMATFTGN
                     REQIRNEFRYLSKCFNDVRNTYFRDNENFKEISMGMSGDYEIAVEEGSTIVRIGSLIF
                     GAPVKNNKQHT"
     gene            6796..7773
                     /locus_tag="PK288_08850"
     CDS             6796..7773
                     /locus_tag="PK288_08850"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPM19071.1"
                     /translation="MEKLQTKYLGLTLSSPLIVSSSLLTSTAGQLKEAEDSGAGAVVL
                     KSLFEEQITSQVHSLSSAHEYPEAADYIANYVRSNSVDNYLDLIRKAKKNLKIPVIPS
                     VNCYTAEGWTGFARKMEDAGADALEVNVFFLPVDRKKTSAESEKLYLDLIEKLRRSIT
                     IPISLKIGFRFSNILYMIDQFYMRGVAGVVMFNRFFEPDIDINKMEIIPASVFSKEFE
                     RRYVLRWIAMASAQDVKIDISASTGVKSGQDAVKYLLAGANTVQVCSVLYEKGISYIR
                     TMNEQILKWMKTKEFDSVDVFRGMLNYMNYDMPAVYERTQFMKHFSTYE"
     gene            complement(7784..8296)
                     /locus_tag="PK288_08855"
     CDS             complement(7784..8296)
                     /locus_tag="PK288_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19072.1"
                     /translation="MTLNEKKLKELGAVLDKNNSLRITQAIRVMRNEPPFSGAIALLV
                     AHYNKSTSYQIRQLIREFMNDMKDQASCGEVVAEIKGSHSSDTLQMIASSCWQSGLDY
                     SGYCHEFADLFLAGDYMTALECFTVIDASAHRLSGDVKKSLIEKIKKGACSITGEKNA
                     LCNELISVLQ"
     gene            8413..>9035
                     /locus_tag="PK288_08860"
     CDS             8413..>9035
                     /locus_tag="PK288_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19073.1"
                     /translation="MNKFFKMDNGVPALVILFAILLSACNSGGKSVKSEGATFRATRD
                     NAALYDDLKKAEKIFYTLPSPLESAMLIKSAGALFDNTILNPVDNAKNYSTNRSMALN
                     LGIFTCDLSFVSLYDQTQMIIDYMNAAKKMADGLGILDAISEEDISKLEENIHNQEVI
                     MEIISQTYLNSNSYLEDNGQPAIAAMVLTGGWIEGLYLSTKLVSPKD"
BASE COUNT         2588 a         1995 c         2184 g         2268 t
ORIGIN      
        1 tgttttgttt atatttgtta tgctgcattc tttaattgga ataaatatgt caaccacacc
       61 cggattctct atcagatagt ctttgacagt aaaaactaca cctatttcct taataaggaa
      121 aatcaaggcc ctctttttgg atatcttgtc ggcatgttaa gcaaaagagg atgccatcct
      181 taccggatgg gagaatatgg aatatgatat tcagtgtttg atctgttaag atattcaagc
      241 catttgttgc caaagaacta tttcaacgta tctgataaag ttgttaattt gttttcaatc
      301 cgggtagtca ttgtaatttt tgtattggta gcttagtgtt aaataacaga acatcagggt
      361 tgtatattaa tatttcatcc cttgtttatt tataataaca acttaacata taaacagtca
      421 attagttagt attttattgt catgtcctaa gaatatttat gttaaattta tatcataata
      481 atttttggct ggcccccaac ccgggcatga ttgcccgtgc gccggacgga acagcctgat
      541 aaccgatgaa aatcaaaatc tgaataatat gaaaaataca atctttaccc gttttcacga
      601 ggctgccgga gccaggatga tcccatttgc cggctttaat atgcccgttg aatttgcggg
      661 aataaatgag gaacacgtgg ctgtaaggga aggcgttggc gtgtttgatg tttcacatat
      721 gggtgaattc tgggtcaccg gacctgatgc gctccctttt ctccggtaca ttgcctccaa
      781 tgatgcagct tcacttactg acgggaaagt acagtatacc tgctttccta acgggaaagg
      841 cgggatagtg gatgattttc tggtttacag gttcaatgca gaaaaatatc ttcttgtggt
      901 gaatgcggct aatattgaaa aggactggga gtggtgtgta tcgcaggccg gccggttcgg
      961 gatcaggccg ggaaaggatc ttgtcaatgc atctgacgaa atatcacagc ttgcggtcca
     1021 gggtcccctg gccctgaaag ccatgcagaa actcactgca gctccggttg ctgaaatgga
     1081 atggtacaca tttaaaataa tcgagtttgc cggcataaag gatgttattt tctccacgac
     1141 cggatatacc ggcgccggcg gatgtgaaat atatgtgagg aatgaggatg gcccgaaact
     1201 gtgggaagct gtttttgacg ccggccggga attcaatatc cgttccgtcg ggctgggagc
     1261 aagggataca ctcaggctcg agatgggcta ttgcctatac ggaaatgata taaatgatca
     1321 gacctcgccg attgaggccg gactgggatg gatcaccaaa ttcagcgacg acagggattt
     1381 tgtcgacaag gctttactcc tgcagcagaa aaaggaaggt gtaaaaagaa ggctgaaagg
     1441 atttgttatg atcgaccgtg gaattccccg tcagcattac gaagtcgtaa atgcatctgg
     1501 ggaaaggata ggtgaagtaa cctccggtac catgtcgccc atgctcaggc aggggatagg
     1561 aatggcttac ctcgatgaag gatactggaa accggatact gaaatattta tcaggataag
     1621 gaacaaggat ctcagggcga gagtggtgaa tcccccattc tataaaaaag actgagagcc
     1681 ggtacgttcc cgcgttgatc tgcctagaat gcggaagaaa gtttacgggg ccaaacccgc
     1741 tgttccgcat ttggagattc aagataatac tgatggaaaa gagaaaactc gaaacctgct
     1801 tttctcccgc tttgtttgag cctgaactgc ttcatgatcc gatagtggtg ataatcgata
     1861 ttctgcgggc ttcatcgtcg atctgcactg ctttcgccaa tggggcagcc aggatcatac
     1921 cggtcgggac agtcggcgaa gccagggccc ataaggagaa cggcttcatg gtggcagccg
     1981 aacgtgatgg ctatgtgctt gattttgctg attttggcaa ctcaccttat aatttcacaa
     2041 aggaaatggt cgaaggccgg accattgtat acagtaccac aaacggtacc ggaataatca
     2101 aactggcctc atcggccagc tgtattgtta taggttcctt tcttaattta agttctcttg
     2161 ttaaatggtt aacagcaaag gataagaatg tactgatatt ctgtgccggc tggaagaaca
     2221 ggttcaacct tgaagacacg atctgcgccg gggcaatagc agagaggctg atgtctactt
     2281 cgctctattc caccatctgc gattcaactc acgcagccat cgacctctgg ctgcttgcaa
     2341 ggaatgatgt catgggatat atagagaagg ctgcccagcg gtcgcggctg agagaaaagg
     2401 gcctcgacga ctgccttgag ttctgtcttt catgcgactt cacggatgtg atacccgtct
     2461 tgcgtaacgg ggtacttgaa actgttgatt tgagtgacca ggcatgatat tttccccgga
     2521 tcaccgaaca aacatattat ttaattaatt ttgtatacag acgaaccaca aaaatttaat
     2581 aaaatcaaat tattcagacc atgaaaaaac acaactttta cgcgggcccg tcaatccttt
     2641 cccaatacac aatcgacaag accattgaag gcatcaggga ttttgcagga tccggactct
     2701 cggtacttga aatatcacat cgcagcaagg aattcattgc ctgcatgaat gagaccattg
     2761 cacttgttaa ggagttgctg gatgttcctt caggttacca ggtaatattc ctcggcggag
     2821 gggccagcct tcagtttgca atggtcccca tgaacctgat gaacagcaaa tcggcgtatc
     2881 tcaataccgg tgagtgggca aacaaggcac ttaaggaggc aaagttgtat ggcgaagtga
     2941 cagaggtggc atcgtcgaaa gaaagcaact acaattacat tccaaagaat tatgtggtgc
     3001 cttctgatgc cgactatttc catgtcacca cgaataacac gatattcggg accagcctta
     3061 aatccgaccc cgacgttccg gtgccgctcg ttgccgatat gtcgtcggat attttcagcc
     3121 gcccggttga tgtgtcaaaa tattcgctga tctatgcggg ggcccagaaa aaccttggcc
     3181 cggcaggggt taccgtggtt ataatcaagg aatccgttct cggcagggtt aaccgtgcca
     3241 taccgacaat gcttgactac cgcacccata tcaaggccga atcgatgttc aatacccctc
     3301 cggtatttgc agtatatgct gcccagcaga cactggtatg gctcaaggga ttgggaggag
     3361 tgaaagccat gcacaaaaag aataccgaaa aggccaggat gctttatgac gagatagacc
     3421 ggaacaaact gtttgttccc acaattaagg atgaggagga ccggtcgatt atgaacatct
     3481 gcttcgttat ggccgacgga tacaaggaac ttgagaaacc tttctcggaa tttgccaaat
     3541 cacgcggaat cgtggggatt gaaggacacc gttctgtcgg aggattcagg gcttcaacat
     3601 acaatgccct ccctgttgaa agcataactg ccctcattga tgccatgaaa gaatttgaaa
     3661 gtaaaaactg attccgggaa acattgttac ctgtttgtat acataattcc ggtaattaaa
     3721 atccaataca atgaaagtac ttgtagctac tgaaaaacct tttgcagaaa ttgccgccag
     3781 gaaaataaag gaactcatag atgcagccgg ttatgaagcc gtattcctgg agaattacaa
     3841 atcagcctcg gaatttgcat ccgcagtttc cgatgccgat gccctgatcg ttcgcagcga
     3901 tattgtatcg gctgaactgc ttgatgcggc aaaacgcctt aaaatagtgg tcagggccgg
     3961 tgccggatat gacaatctcg accttgaagc ctgtacgcgt cataacgttg tggcaatgaa
     4021 cactccggga cagaattcaa atgccgttgc agaacttgcg tttgaaatga tgctttatat
     4081 ggccaggagc ggattctcag gtaaatcggg aaccgaattg cggggaaaga cgatcggcat
     4141 ccatggtttt ggaaatgttg gccggtatgt tgcagaaata tccggaggtt tcggtatgac
     4201 tgtcagtgca ttcgatccct atattgcaga ggatgttatt acagggtatg gcatcagggc
     4261 ctgtaaatcc gcaaaggaac tttattctga atgccagtac atatcgctca atattccgtt
     4321 taacaaggaa acaggctctt ccgtcaacta cagccttctt tcagggatgc ctgtaccggc
     4381 ggtactcgtc aacaccgcac ggaaagaaat catcgacgag gaatccctgc tgaaaatgtt
     4441 cgcggaacgc cccgatttca ggtatctttc cgatgttgca cccgactgca aggctcttat
     4501 cgaggagaag tacagcggaa gattcctttt tactgcaaag aaaatgggag ctcagacaga
     4561 ggaggcaaac agcaatgccg gtattgccgc aataaaccag atagtcgatt atttcaaaac
     4621 cgggaatcag aagttcaggc ttaacaagta aaaggatcgt gaccagcagc gccggaacat
     4681 caccaggttt ttgatataat taaatattaa tgattcaacg atcatataag aaatggctat
     4741 agtcaaacca ttcaggggcc tcagaccccc ttcaggcatt gtcagcgatc tggcgtgcct
     4801 gccctatgat gtaatgaaca caggtgaagc aagaaaaatg gcagagggca aagaatgctc
     4861 cctgcttcac atcacacgcg ccgaaataga atttcctcct gaaaccgaca tccattcgga
     4921 agaagtatat gaaagatcca ggcaaaactt tgccctctgg caaaaaaagg gatggctgat
     4981 tcaggacgac cagccatgct attacattta tgcccagaca atgaagggca gaacccagta
     5041 cggaattgca ggttgcgctt cggtcgatga ttacctcgac ggaaccatta ggaaacatga
     5101 actcaccagg cctgataagg aacaggacag aatggttcat ataagggtta acaatgccaa
     5161 tatggaaccg gtatttattg cctatccttc agtcagcgag atcgatgaaa tagtcagttc
     5221 aattgtggag aagagtacgc cggaatatga ttttatctct gacgacggca tcggacatca
     5281 tttctgggtg gtgaataagc ctggtgtcat caggcgcata gaagagcttt ttgctcagaa
     5341 ggtgccatat acctatgtgg ccgacggaca ccacaggaca gctgcggctg ccctcgtggg
     5401 caaggaaaag agagaacaga atcccggata tgacggtacc gaggaattca attttttcct
     5461 ggccgtacat ttccccgaca accagctcgg gataatggac tataaccgga ccgtaaaaga
     5521 ccttaacggc ctgactgagg aagagttcct tcaaaagctg aatgacggat tcatcgtgac
     5581 tccgaaaggt acaagagcct accggcccgc caggctccac aacttttcaa tgtacctcgg
     5641 agggatgtgg tacagcctta ccgccaggga aggtacctat gatgacaatg atcctctcgg
     5701 tgttcttgac gtaacaatac tgaccgagca ggtattgaag ccgatcctcg atatccagga
     5761 cctcaggcgc tcaaaaagga ttgatttcgt gggcggcatc agaggactga acgaacttgc
     5821 gaagagggtc agccagggtg aaatgaaagt tgcattcgca ctctatcccg tttcaatgaa
     5881 acagcttatg gcaattgccg attcgggtaa cataatgcct cccaagacca cctggtttga
     5941 acccaaattg cgcagcggac tcgtaatcca tctgctggac taactgcctg cctgctcctg
     6001 caaccggccg ggtgctccgg ctaataacgg ctgatgatcc cggaacctga accggccggc
     6061 cgggaaaaga acttgtttaa ggaggactgt aaagattatc tgcgtgactg acatcgcttc
     6121 aaatatcaga tcggtaaaaa cggcattacc ccccggggtt aagcttgtgg cggtgtcgaa
     6181 ggccaaatcc ccagccgaga tacttgaagc ctacaatgcc ggccaaagat gctttgggga
     6241 gaacagggta atggaacttg cagggaagaa ggatgttctt ccccctgata ttgaatggca
     6301 cctgatagga caccttcaga caaacaaggt taaatacctg gttccgttca tcagcatgat
     6361 agagtcggtt gactctttcc ggcttctaaa gataattgac aatgaagcct ccaaagtgaa
     6421 cagggtgatc agctgtctcc ttcagatcca tatagcagaa gaagagacca aattcgggtt
     6481 cactatggat gaactgtatg ccatgctgga aaacgctgac ctttataagc tcagaaatgt
     6541 caatatctgc ggcgtgatgg gaatggccac gtttaccggc aacagggaac agataagaaa
     6601 cgagttcaga tatctctcaa aatgtttcaa tgatgtcagg aacacttatt tccgggataa
     6661 tgagaatttt aaagagatat caatgggaat gtcgggcgat tatgagattg cggtggagga
     6721 ggggagtaca attgtgcgga tcgggagtct tatattcgga gcccctgtaa aaaacaacaa
     6781 acaacatacc tgatcatgga aaaacttcag acaaaatacc tggggctgac tttaagcagt
     6841 cccctgattg tcagcagcag ccttctcaca tcaacagcag ggcagcttaa ggaagctgaa
     6901 gacagcggtg caggcgccgt tgtcctgaaa tccctctttg aagaacaaat cacttcgcag
     6961 gtccattcat tgtcatcagc gcatgaatac cccgaagcag ctgactatat agccaattat
     7021 gtgagatcaa actctgtcga caattacctc gacctgataa gaaaagccaa gaaaaacctg
     7081 aagatcccgg ttatcccgag tgtcaattgc tacacagccg aaggctggac cgggtttgca
     7141 aggaagatgg aagatgccgg tgccgatgca ctcgaggtaa atgtgttttt tcttccggtc
     7201 gacaggaaaa aaacatctgc tgaatcggaa aagttgtatc tcgatctaat tgagaagctc
     7261 aggcgcagca taacaatccc catttccctg aaaatcggat tcaggttttc caacatcctt
     7321 tacatgatcg atcagttcta catgaggggt gttgccgggg ttgttatgtt caacaggttc
     7381 tttgagccgg atattgacat aaacaaaatg gagatcattc cggcttctgt tttcagcaag
     7441 gaattcgaaa gaaggtacgt tctgagatgg atcgccatgg caagtgccca ggatgtaaag
     7501 atcgacatct ctgcctcgac gggtgtcaaa tcaggacagg atgccgtgaa atacctgctt
     7561 gcgggagcga ataccgtaca ggtctgctct gtactgtatg aaaagggaat cagctacatc
     7621 cggacaatga acgagcagat actgaaatgg atgaaaacga aggagtttga cagtgttgat
     7681 gttttcagag gcatgctgaa ctatatgaat tatgatatgc cggcggtata tgaacggacg
     7741 cagttcatga agcatttctc aacatacgaa tagtgcgcca cccttactgg agaacagaga
     7801 tcagttcatt gcacagtgcg ttcttttcac cagtgatgct gcaggcacct ttctttatct
     7861 tctcgatcag cgattttttc acatcaccgc taagcctgtg tgccgaggcg tcaatcacgg
     7921 tgaaacactc aagggccgtc atatagtcgc ccgccaggaa aagatccgcg aactcatggc
     7981 agtaacctga ataatccaga cccgactgcc agcacgacga cgcaatcatt tgcagggtgt
     8041 cgctgctgtg actccctttt atttccgcaa caacttcccc gcatgaagcc tgatccttca
     8101 tatcattcat gaattccctt ataagctgcc ttatctggta acttgtgctt ttgttataat
     8161 gcgcaaccag cagggcaatt gccccggaaa acggcggctc atttctcatt accctgattg
     8221 cctgcgtaat cctgaggctg ttgtttttgt ccagaaccgc accaagctcc tttaatttct
     8281 tctcatttaa tgtcattttt gaatattttc tgcaaagtta ccataccatt ttacagtttt
     8341 aaccgttttg atataataaa aataaagtac taatttagct tagtttagcc tgaaaatata
     8401 aaattgccaa tcatgaataa gttttttaaa atggataatg gtgtgccggc actggtaatc
     8461 ttgtttgcga tattgctttc cgcatgcaac tcgggaggga agagcgttaa gagtgaagga
     8521 gccacgttca gggcaacccg cgataatgct gccctgtatg acgatttgaa gaaagcggaa
     8581 aaaatattct ataccctgcc gtcaccactc gaatcggcaa tgctgataaa gtcggccggc
     8641 gccctgttcg acaacacgat cctgaacccg gtcgacaatg caaaaaatta cagtacaaac
     8701 agaagtatgg cacttaacct cggcatattt acctgcgacc tcagtttcgt gagtctgtac
     8761 gaccagacac agatgataat cgattatatg aatgcggcca agaaaatggc cgacggtctt
     8821 ggcattcttg atgccatttc ggaggaagat atcagcaagc tggaggaaaa catacataat
     8881 caggaagtga taatggagat tatttcgcag acttatctca attcaaactc atacctcgaa
     8941 gataacggtc agcctgcaat agctgccatg gtactcacgg gaggctggat cgaaggactg
     9001 tacctttcaa caaagctcgt gagtccgaag gatat
//
LOCUS       DAOOYG010000104         8913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_8628_length_8913_cov_6.743396, whole genome shotgun sequence.
ACCESSION   DAOOYG010000104 DAOOYG010000000
VERSION     DAOOYG010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8628_length_8913_cov_6.743396"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..7542
                     /gene="sprA"
                     /locus_tag="PK288_08865"
     CDS             97..7542
                     /gene="sprA"
                     /locus_tag="PK288_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPM19074.1"
                     /translation="MIRLRSEVSYKTLLRLLYCLLFASPALWSSETSGQVNVSAPLPD
                     TARKSPLKLYDISGIPWEPAPKAPLFLDNPSNVKYSVVYDPVKNEYVIYQKVGLFDYR
                     TPVHMSPEEFRKYQFNKEMRDYWQSRISGQEPGYRSGLIPQIEIGGEAFDKIFGSNVI
                     NIVPQGSAELIFGINISKTQNPTLPERLQTIPTFDFKEKIQMNVTGTIGDKMELGVNY
                     NTDAMFDFENKTKLQYSGKEDEIVRKVEAGDVTLPLTGTLITGSHSLFGLKTELQFGK
                     LNVTTVLSQQKGETSTIQVEGGAQSNSFEIFADDYESNRHFFLSHFFRDIYDDALKNL
                     PLVSSGVNIERIEVWITNKTSSFDETRNIVAFSDLAENAGHIYNKIPAFQAGPGAERY
                     PDNDANQLYNQLNTSYSLMRNVDQVANAFNSLYPGFQIGRDFEKIENARKLNDREFTL
                     NKQLGYISLNTALNTDEVLAVAFEYTLNGKVYKVGEFSTDGIAAPQTLILKLLKGTTL
                     SPRYPAWNLMMKNVYSLGSGRLETNDFQLNILYEDDKTGNAINYLPEGRLANKILLQV
                     LGLDNLNSQLDHVPDGYFDFIDGVTVMISRGKIIFPVIEPFGSYLAKQIGDPVLASKY
                     VFQELYDSTQTIAKQMAEKNKFKLTGHYTSESGSEIRLNATNIPVGSIIVTAGGVTLT
                     ENTDYTVDYNLGTVTIINPALIESQTPIKVSLESNQFFGFQTKTLLGTHLDYKFSNNF
                     NIGGTILHLNERPYTQKVNFGEEPISNTIWGLNTSYRSESQLLTKLIDKIPLLETKTP
                     SSISFNGEFAHLIPGHSRAISSAGNSYIDDFESSEIPLDLKSFNAWSISSIPQGQDNL
                     FPEARLNNNLASGYNRAKLAWYVIDPLFLRNGSSTPSHIKQNPDEQSSHFVREIYENE
                     LFPNRESSTGIPNTISVLNVAYYPDERGPYNYDVESGPYSRGLTAEGRLSDPRTRWGG
                     IMREVLTSDFETANIQYLEFWMMDPFVEDPEHNGGDLYFNLGNISEDILKDSRKSFEN
                     GLPGSPLVQNVDTTAWGRVPTVQSVVKAFDNDSESRRYQDIGLDGLGNEDEQSFFSDY
                     LSRVEGITNPQAFQQILKDPSGDDFHYFRGSDYDFEQKSILERYKNYNGLEGNSPTSG
                     MSPESYPTSGSTLPDMEDINRDNTLNETESYYQYKVSLRPSDLKVGSNYIVDEIEAEA
                     TFANGEKSRVKWYQFKVPVTEYQKRIGVISDFKSIRFIRMFMKDFSKPVIMRFAELNL
                     VRAEWRKYNIDFTEGGERITIPEATDGTFEIASVSIEENASKQPVNYVLPPGFNRIVD
                     PQNPQLRQLNEQAMVLKVQDLADGDARAAYKNVNLDIRQYKSLRMEVHAEALIGQPLN
                     DDELTAFIRLGSDYRSNFYEYEVPLKLTPPGDRYNNDSEADRAIVWPQANRFIVNLSV
                     LQEAKKERNRKMADPGSALSLNDVFAFADDSNRVYVCGNPNLSNIKVIMVGVRNPIRT
                     RNPGVDDGNPKSGEIWVNELRLSDFIENGGWAANTHLQARLADLGTVDFVGQMSTPGW
                     GSIDRKVNERSKAEIIQYDISSTLELGKFFPEKLGVRLPVYVGYSESRIKPQYNPLDP
                     DIELKDALREAPDKETRDSIRNIAEEYSRRKTISINNAGITKRGEKSHAWDLANFSVN
                     YTYNETYNSSTTTEIDLERNYRGGINYNYEAQPQNVMPFKNVKFLNSPLLRIIKDFNF
                     YPLPRSISFRTDMSRYYNEVKTRNINNPYLLISPTFRKDFEWSRMLDIKYDVTRQLKI
                     DFTSNNIARIDEPEGGVDKRRYRSEYENWRDSVLTNLKNLGRATMYNHFFNATYNLPV
                     NKLPMLSWVNANARFGSGYTWQAGSLYPDSVKINLGNTIQNNNELTFTAMANLATLYS
                     KSKFLKNIENNTRPDAALRMKKEYRTVTFTRNNVSFKAGAVRSIVHNLKTRDITVKIV
                     RKDGIDLSGKIDIVNENRINFTASESAEGTQVIIEGRLPQKRNPLTVTGEYFLRALIG
                     VRSVSLTYTSSQGQFLPGFLPEARFLGMSRDNNNISAPGLPFILGFSEKNFFDKAAAR
                     GWITTDTLLNTPAVYMNRNDLSVRSIIEPFPGLRIDINADRRFQENINSYYTADYNGN
                     FPDSTRNRIVNGSFSISIISWGSAFEKISKDNDYASRAFENFKKNSIIISERRAAARQ
                     SIDPGYDPDVNPGTGEQLEGLYKSGYGATSREVLIPAFYAAYTRTDPKKVDLSVFPSA
                     FRMMPNWRLTFDGLSKFQFVQKVFRSINLSHQYRSVYQIGSYTTNLNFLADDNGLSRL
                     RDLQDNYIQQYEINVVSVSEQFSPLINLDFNWKSNLTSRFEWRKSRTVALNLTSNQVT
                     DARINELVFGAGYRFDDVQIIIKTGGSQRVLKSDLKIRFDLSIRDNKTIARKLIENVN
                     QPVAGIKVFTIRTTADYVLSDRFNLQLFADHTVNNPFVATTFPTSNTNFGFSLKFTLA
                     Q"
     gene            7695..8069
                     /gene="gcvH"
                     /locus_tag="PK288_08870"
     CDS             7695..8069
                     /gene="gcvH"
                     /locus_tag="PK288_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPM19075.1"
                     /translation="MVVPESLLYSKDHEWLRVEGEFAYVGITDFAQGELGDIVFVEIE
                     TLGETLKKEEVFGTIEAVKTVSDLFMPVGGEIVEVNPYLENSPDIVNKDPYGNGWMIR
                     IKITNPDEIGELLSSEKYRSML"
     gene            complement(8064..>8913)
                     /locus_tag="PK288_08875"
     CDS             complement(8064..>8913)
                     /locus_tag="PK288_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HPM19076.1"
                     /translation="AMYNERGHKVEKTGPATPAVILGLNGAPQAGDKFNIMESDREAK
                     DIATKRAQLQREQDLRTQKHITLDEIGRRIAIGNFQELNIIVKGDVDGSVEALSDSLI
                     KLSTPQIQLNIIHKAVGQISESDILLAAASNAVIVGFQVRPSLNARKLAEKEEIDIRL
                     YSIIYDAIEEIRAAMEGMLSPEVKEEIVATLEIREIFKVTKVGTVAGCYVKEGKITRN
                     TKIRLIREGIVIYTGELGSLKRFKDDVKEVVGGYECGLNIANFNDIKIGDIVEGYQEI
                     EVKKSL"
BASE COUNT         2617 a         1965 c         2023 g         2308 t
ORIGIN      
        1 gttattaaag tccttgacaa aataatcagg gaagaaaaag acctgactgt tgaagacatt
       61 atcaaaaaag cactcaagag cctgtaaata agaatattga ttaggttgag atcagaggta
      121 tcatataaaa ccctgctgag attattatat tgtttgctat tcgcttctcc tgccctctgg
      181 tcatctgaaa caagtggtca ggttaatgtt tcggcacccc tgcccgatac tgccaggaaa
      241 tctcccctca aactctatga tatttcgggg ataccatggg agccagctcc aaaggccccc
      301 ttgttcctcg ataacccatc aaatgtaaag tattcggttg tttatgatcc tgtaaagaat
      361 gaatatgtga tttatcagaa ggtgggactt tttgattaca ggactcccgt gcacatgagc
      421 cccgaggagt tcaggaagta ccagtttaac aaggagatgc gtgattactg gcagtcacgt
      481 atcagcggcc aggagcccgg atacagatcg ggcctcatac cacagataga aatcggagga
      541 gaagctttcg acaagatctt cggtagcaat gtgatcaata tcgttcccca gggatcagcc
      601 gagctgatct ttggcattaa catatcaaaa actcaaaacc ctactctgcc tgaaagactt
      661 cagacaatcc cgacttttga cttcaaggaa aagatacaaa tgaatgtcac cggaaccatc
      721 ggcgacaaaa tggaactggg tgtcaattac aacaccgatg caatgttcga ttttgagaac
      781 aaaacgaaac tgcagtattc cgggaaggag gacgaaatag tgcggaaggt cgaagctggc
      841 gatgtaaccc tgcccctgac aggaaccctt attaccggaa gtcacagtct cttcgggctc
      901 aaaaccgaat tgcagttcgg caagctgaat gtaactaccg tactttccca gcaaaaaggg
      961 gaaacttcca ccatccaggt tgaagggggg gcacagtcga acagtttcga aatattcgct
     1021 gacgattatg agtcaaaccg tcattttttt ctttctcatt tcttcaggga tatatacgat
     1081 gatgcattga aaaatcttcc gttggtgagc tcaggtgtga atatcgaaag gattgaagtt
     1141 tggattacca ataaaacaag cagttttgac gaaacaagaa acattgtggc gttttcggat
     1201 ctggcagaaa acgccggaca tatttacaac aaaattccgg catttcaggc cggtcccggt
     1261 gcagaacggt atcccgacaa tgatgcaaac cagctttaca atcagctgaa cacttcttac
     1321 tcactcatgc ggaatgttga ccaggttgcc aatgcattta actccctgta ccccggattc
     1381 cagattggaa gggactttga aaagattgag aatgcaagaa aactgaatga cagggaattt
     1441 accctgaaca agcagcttgg atacatatca ctgaacactg ccctgaatac tgatgaggtg
     1501 cttgcagttg cctttgagta tacccttaac ggaaaggttt acaaagtcgg agaattctcc
     1561 accgacggaa tagctgcgcc gcagaccctg atactgaagt tattgaaagg aacaacactt
     1621 agccccaggt atcctgcctg gaatcttatg atgaagaatg tatactcgct gggatcaggc
     1681 aggctggaga ctaatgattt ccagttgaat atactttatg aggatgataa gacgggtaat
     1741 gcaattaact atcttcctga aggcaggctg gcaaacaaga tcctcttgca ggttcttggc
     1801 ctcgacaacc tgaattcaca gcttgaccat gtccccgacg ggtattttga ttttattgat
     1861 ggcgttaccg taatgataag caggggaaag ataatcttcc cggtcattga acctttcggc
     1921 agttatctgg caaaacagat cggggaccct gtactggcct caaaatatgt cttccaggag
     1981 ctgtatgatt ccacacaaac cattgccaaa cagatggctg aaaaaaacaa gttcaaactg
     2041 acggggcact atacttcgga gtcgggttca gaaataagac tgaatgctac aaatatacct
     2101 gtaggttcaa taattgtcac cgcgggaggg gtaaccctca cggaaaacac cgattacact
     2161 gtcgattata atctgggtac cgttacaatt ataaatcctg ctttaattga atcccagact
     2221 ccgatcaaag tgagcctgga gagtaatcag tttttcgggt tccagacgaa aaccctgctg
     2281 ggcacccatc tcgattacaa gttctcaaat aattttaata tcggcggaac gatactccat
     2341 ctgaatgagc ggccatatac ccagaaggtc aattttggcg aagagccgat ttcgaatacc
     2401 atctggggcc tgaatacctc gtacaggtca gaatcacagt tacttacgaa actaatcgat
     2461 aaaattcccc tccttgaaac caaaacacca tcaagcatct ctttcaacgg tgaatttgcc
     2521 cacctaattc caggtcattc cagggccatt tcaagtgcgg ggaattctta tatagatgat
     2581 ttcgaatcaa gtgaaatacc tcttgatctg aaatcattta acgcatggtc catttccagt
     2641 attcctcagg gacaggataa cctatttccg gaagcccgtc ttaacaacaa tctggccagc
     2701 ggttataaca gggcaaagct ggcgtggtat gtcattgatc cccttttcct gaggaacggc
     2761 tcttccactc cctcacatat aaaacagaac cctgatgagc aatccagtca ttttgtgagg
     2821 gaaatttatg aaaatgagct tttcccaaat cgagagagca gtacaggaat ccctaatacc
     2881 atttctgtcc tgaatgtagc ctattatcct gatgaaaggg gcccctacaa ctatgatgtc
     2941 gaatcaggac cgtattccag gggcctgaca gcggagggaa ggctcagtga tccaagaacc
     3001 agatggggag gaatcatgcg tgaagtgctt accagtgact ttgagactgc aaacatccag
     3061 tacctggagt tctggatgat ggatcccttt gtggaggatc ctgagcacaa cggcggggac
     3121 ctttatttca atcttggaaa tatttccgaa gatattctga aggattcccg taaaagtttt
     3181 gagaacggcc ttccgggatc tcccctggtg cagaatgtgg ataccacggc ctgggggaga
     3241 gtacccaccg tacaatcggt tgtcaaggct tttgacaacg attcggaatc gaggcgttac
     3301 caggatatcg gccttgacgg gttgggaaac gaggatgaac agagtttctt ttcggattat
     3361 ctgagcaggg tggaaggtat aacaaatcct caggcgtttc agcaaatatt aaaagatcct
     3421 tcaggtgatg attttcatta tttccgggga tctgattacg attttgaaca gaaaagcatc
     3481 cttgagcgat acaagaacta taacgggctt gaaggcaatt cccccacttc ggggatgtcg
     3541 ccggaatcct acccgacatc gggatcaacc ctgcccgaca tggaggatat taaccgtgac
     3601 aataccctga acgaaacgga aagctattac cagtacaagg taagcctcag gccgtcggat
     3661 ctgaaggtag gatccaacta tattgtcgat gagatcgaag cagaagcaac ttttgccaat
     3721 ggagaaaaat caagggtgaa gtggtatcag ttcaaggtcc ctgttactga ataccagaaa
     3781 agaattggtg taataagcga tttcaagtca atacgcttta tcaggatgtt tatgaaggat
     3841 ttcagcaaac cagtgataat gcgttttgca gagcttaacc ttgtgagggc cgaatggagg
     3901 aaatataata ttgatttcac tgaaggcggg gagcgtatca ctattcccga agccactgac
     3961 ggaacctttg agatagcttc ggtgagcatt gaagagaatg cctcaaagca gccggtgaac
     4021 tatgttttgc cacccggatt caacaggatc gtagacccgc agaatccgca gctgcggcaa
     4081 ctgaatgagc aggcgatggt tctgaaggtt caggacctgg ctgacgggga tgcccgtgca
     4141 gcttacaaga atgtcaacct cgatatcagg cagtacaaga gcctcagaat ggaggtacat
     4201 gcagaagccc tgatcggtca gcctttaaat gacgatgagc tgacggcctt cataagactg
     4261 ggttctgatt acagatcgaa cttttatgaa tatgaggttc cgctgaaact tactccccct
     4321 ggcgacaggt acaataacga ttcagaggct gacagggcaa tagtctggcc ccaggctaac
     4381 cgtttcattg tgaatctgtc ggttctgcag gaagcaaaaa aggaaaggaa caggaagatg
     4441 gctgatccgg gttcagctct ttccctaaac gatgtctttg cctttgccga tgattccaac
     4501 agggtctatg tatgcggtaa tccaaacctc agcaatatta aggtgattat ggttggggtc
     4561 aggaatccaa tccgaacccg caatcccgga gttgatgatg gtaatcctaa atcgggagag
     4621 atatgggtaa atgagctcag gctgagtgat tttatcgaaa acggcggatg ggcggccaat
     4681 acgcacctgc aggccagact ggccgatctg ggaacagttg actttgtggg acagatgagc
     4741 actcccggtt gggggagcat cgaccggaaa gtgaatgaaa ggagcaaggc agagattatt
     4801 cagtacgaca tttcctcaac acttgagctc ggcaaattct tcccggaaaa acttggtgtc
     4861 aggctgccgg tttatgtggg atactccgag tcgcgaatca aaccccagta taatccgctc
     4921 gatcccgaca ttgaacttaa ggatgctctt agggaggcac cggacaagga aacacgggat
     4981 tcaataagaa atattgcaga agagtattca cgaaggaaaa cgatttcaat taataatgcg
     5041 ggaatcacaa aacggggcga aaagtcccat gcatgggatc ttgccaactt cagtgtcaat
     5101 tatacctaca atgaaacata caattcaagc accaccactg aaatagatct ggaaaggaat
     5161 taccggggcg gaataaacta caactatgaa gctcagccac agaacgtgat gccgttcaaa
     5221 aatgtgaagt tccttaattc tccgcttctc agaataatca aggattttaa tttttatccc
     5281 cttccaagga gtatttcctt cagaacggac atgagcaggt actacaatga ggtcaaaacc
     5341 aggaatatca acaatccata cctgcttatc tctccaactt tcagaaaaga ttttgaatgg
     5401 agccggatgc ttgacattaa atatgatgta acacggcagc tcaagattga ttttacttcc
     5461 aacaatattg cccggatcga tgagcctgaa ggcggtgttg ataaaaggag atacagatca
     5521 gagtatgaga actggcgcga ctcagtactt accaacttaa agaatcttgg aagagccaca
     5581 atgtataatc atttctttaa tgcaacctac aaccttccgg taaacaaatt gccgatgctt
     5641 tcctgggtaa acgccaatgc acggtttggt tccggttata catggcaggc cggttcactc
     5701 tatcccgact cagtaaaaat aaatcttggc aataccattc agaacaacaa tgagcttacc
     5761 tttactgcaa tggccaacct ggccacactt tacagcaagt caaaattcct taagaatatt
     5821 gaaaacaata cccgtccgga tgcggccctg aggatgaaga aagagtacag gacagtaacc
     5881 tttacacgta acaatgtaag ctttaaagct ggtgctgttc ggtcaatcgt tcataatctc
     5941 aaaacgaggg atattacggt caaaattgtc aggaaggatg gcatcgatct cagcggaaag
     6001 attgatatcg taaacgaaaa caggatcaat ttcaccgctt ctgaatcagc tgagggaacc
     6061 caggtcatta ttgaagggag attgccccag aaacgcaatc cccttacggt taccggggaa
     6121 tattttttga gggctctcat tggcgtaaga tccgtgagcc tgacttatac ctcatcgcag
     6181 gggcaattcc tgcccggctt ccttcctgag gcaagattcc ttggcatgtc ccgcgacaac
     6241 aataatatca gcgcaccggg ccttcctttc attctcggat tcagtgaaaa aaactttttt
     6301 gacaaagctg cggcaagggg atggatcaca accgacaccc tacttaatac gccagcagtt
     6361 tatatgaaca ggaatgacct gtcggtcaga agtatcattg agccttttcc cggtctgagg
     6421 attgatataa atgccgaccg gcgcttccag gagaatataa attcatatta tacggctgat
     6481 tataatggta atttccccga cagcacccgt aacaggattg taaacggcag tttttcaata
     6541 tcaataatat catggggttc tgcctttgaa aagatttcaa aggacaatga ttatgcctca
     6601 agagcatttg aaaatttcaa aaagaacagc ataatcattt ctgaacgcag ggctgctgca
     6661 cggcagagta ttgatccggg ttatgatccg gatgtgaatc ctgggacagg tgaacaactt
     6721 gaggggttgt acaaaagtgg ttacggcgcc acatcgaggg aggtccttat acccgcattt
     6781 tatgcagcct atacaagaac tgatccgaag aaagtcgatc tttccgtgtt cccttcagca
     6841 ttccggatga tgccgaactg gcgccttaca tttgatggtt tgtctaaatt ccagttcgta
     6901 caaaaggtgt tcaggtcaat caacctttcg catcagtaca ggagtgttta ccagatcgga
     6961 tcctacacaa caaatcttaa tttcctggct gatgacaacg gcctgagcag gctgagagac
     7021 ttgcaggaca attatatcca gcagtatgaa atcaatgtgg tttcggtcag tgaacagttc
     7081 agtccgctta taaacctcga tttcaactgg aaaagcaatc ttacatcaag gtttgaatgg
     7141 aggaaatcgc gtacggtggc cctgaacctg acgagcaatc aggtaaccga cgcaaggatc
     7201 aacgaacttg ttttcggagc aggttacaga tttgatgatg ttcagataat tattaaaacc
     7261 ggaggaagtc agcgcgtact taaaagcgat ctgaaaataa ggttcgatct ttcaataagg
     7321 gataataaaa ctattgcccg aaagttaatt gagaacgtga atcagcctgt ggcaggcata
     7381 aaggtgttta ccataaggac cactgctgat tacgtcctga gcgacaggtt caatcttcag
     7441 ctttttgcag atcatactgt caataatcct ttcgtagcaa caactttccc tacatccaat
     7501 accaattttg gattcagcct taaattcacc cttgctcagt aagcagtaca attctggtca
     7561 ttttgaccat caatcaattg tggcatatct ggcaaattaa gttattgaaa aaccggtcag
     7621 gcaaaaaaaa attcctattt ttaggcagaa aaagtaatca gtttcaaata ttaaccacta
     7681 attaccaaac atccatggtt gtaccagaaa gtttattgta ctctaaagac cacgaatggc
     7741 tcagagtgga aggtgaattt gcttatgttg gcataaccga ttttgcacag ggcgaactgg
     7801 gcgacattgt atttgttgag attgaaacgc ttggcgaaac ccttaagaaa gaagaggttt
     7861 tcgggacaat tgaagccgtg aaaacggtat ctgacctgtt tatgcctgtt ggaggtgaga
     7921 ttgttgaggt taatccgtac ctcgaaaact cacctgacat tgtgaacaag gatccttatg
     7981 gaaacggatg gatgatcagg atcaaaatca ccaatcctga tgaaattggc gaattgcttt
     8041 cttcggagaa ataccggtca atgttataag gatttcttta cttctatttc ctggtaacct
     8101 tcaacaatat cacctatctt gatatcgttg aagttggcaa tattaagacc gcattcgtat
     8161 cctccgacaa cttcctttac atcgtccttg aatcgtttca gggaaccaag ttctcctgta
     8221 taaataacga ttccttcacg gatcagcctg attttggtat tccgtgttat tttgccttcc
     8281 tttacatagc aaccggccac tgttccgact tttgtaacct tgaagatttc ccttatttca
     8341 agtgtggcaa ctatttcctc tttcacttca ggagagagca taccctccat tgcagcccgg
     8401 atctcctcta tggcatcgta gataattgaa tagagcctta tatcgatctc ctccttttcc
     8461 gccagtttcc gtgcgttgag ggaaggtctt acctggaatc cgacaatcac tgcatttgat
     8521 gctgcagcca gaagtatgtc ggactctgat atctgtccca cggccttatg aatgatatta
     8581 agctgtatct ggggtgttga aagcttaata agggaatcac tcagtgcttc aactgaaccg
     8641 tcaacatcgc cctttactat tatattaagc tcctggaagt tgccaatggc aatcctcctg
     8701 ccgatttcat caagggtgat gtgtttctgt gttctaaggt cctgctcccg ctgaagctgg
     8761 gctctctttg ttgctatatc cttggcttcc cggtcgcttt ccattatgtt gaacttatcg
     8821 cctgcctgag gagcgccatt caatcccagg atgactgcag gagtcgcagg tccggttttt
     8881 tcaaccttat ggcccctttc attgtacata gcc
//
LOCUS       DAOOYG010000105         8902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_8644_length_8902_cov_7.869334, whole genome shotgun sequence.
ACCESSION   DAOOYG010000105 DAOOYG010000000
VERSION     DAOOYG010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8644_length_8902_cov_7.869334"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1068)
                     /gene="gldL"
                     /locus_tag="PK288_08880"
     CDS             complement(124..1068)
                     /gene="gldL"
                     /locus_tag="PK288_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HPM19077.1"
                     /translation="MSLAELVQSSGWKNFMAKLYGLGASIVIIGALFKIQHWPLAGLM
                     LTVGLCTESIIFFFSAFEPLHEEVDWTLVYPELAGIPNDEPGALGARDSKYQGGHAGG
                     GYAVGGGISGGGYSGGGYGGGGYGGGGGSVALSKFDEMLEKAEITPELFQKLGVGMQK
                     LSEATSNINVMGDISAASAKYMNTIHAANESLNRLSETYQAASKTINDTQLVYGKMAE
                     TVSFIDTGGKNYHQQLETLNKNLSALNAVYELQRKGADEHIKESERLQKGIQGLIKDL
                     SDSAGDASRYREQIAKLNDNLTALNNVYGNMLAAMNVK"
     gene            complement(1126..2472)
                     /locus_tag="PK288_08885"
     CDS             complement(1126..2472)
                     /locus_tag="PK288_08885"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM19078.1"
                     /translation="MKKVIFSALITIVVLSGCGSSNTGELTGIAGRKNFFEPEPFEMA
                     FIPAGSFEMGPSDQDAVFAANTISKTVTVDAFWMDQTEITNNKYRQFVYYVRDSILRT
                     NLGNAGVPNRNYFMVDREGNVIEPNVINWNERIDPREETTAAVLEEMYYPPEERFNSK
                     KQIDIRKLNYSYFWVDYQQAAKARYVHDYANNTGKYEGYVTNPDGSRTEVKDRSSFIK
                     RNVINIYPDTLCWIRDFTYSFNDPQADLYFWHPSFDDYPVVGVSWVQATAFSRWRTDY
                     MNTALRKKGIADVQAYRLPLETEWEYAARGGLDLSMYPWGGPYTRNYQGCFLANFKPL
                     RGNYIDDGTVYTSKVGSYEPNEYGLFDMSGNVAEWTCCAYHPSAYIFQHDLNPNYEYN
                     ATPQDPPVMKRKVIRGGSWKDIAYFLQTSTRDYEYQDSTKSYIGFRNVRSYIGVNH"
     gene            complement(2517..3461)
                     /locus_tag="PK288_08890"
     CDS             complement(2517..3461)
                     /locus_tag="PK288_08890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPM19079.1"
                     /translation="MKKLYFIFLILVVFQVCGFSQQDALTSNYMFNTLTYNPGFAGIP
                     GMACATAVNRQQWVGFKGAPATTLFNVSAPIAPFKINSGAGLMVKSDKIGFERDISLA
                     LNYSYHMELGQGTLGIGISAGMFNMTLDPVWKIPESDIFTPPSGDPLIPESKESSLAF
                     DAGLGLYYKAEKYYAGLSVTHINQPNIKFSKGITYLSRHYYFTAGYILQLPSPSLELL
                     PSVYTMTDGKVLQFSATSLIRYNKKIWGGVSYRPGDAFAAILGIELSNGIRVGYAYDF
                     PVTDIIRNTSGSHEFMVSYCFDISLGKSPMRYKSIRFL"
     gene            complement(3645..4814)
                     /locus_tag="PK288_08895"
     CDS             complement(3645..4814)
                     /locus_tag="PK288_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HPM19080.1"
                     /translation="MLDLKDYFDPVSIDEPEFLNMTSNAGFPHNISIHTENDRINPGE
                     RFKVVLIGVPDGRNSPNPGSDKAPDAIRQQLYNLAKIPGKIKIADLGNMKQGMAFNDT
                     IAGLTDILVFFMRENIFPVIIGGSSALITAIDRSMSVLKKKYTMISVDPRIDFTSETG
                     AFSSSSYLNDITRAHNRTMEHFINIGYQTYLNDQQVLNRFARNNSELVRIGDVRQAIY
                     LTEPLFRDSEAALFDISSVRQSDAPGTAIPSPNGFYGEEICLLARYAGISDRLKVFGL
                     FDVNPEFDVRGQTTGLAAQILWFFLEGFSQKQYETPALSDTNSGRFIKYHVRVKDLEE
                     DLIFVKSNYTERWWIELTDINSGKVYIACSHEDYLKANKNELPDRWVKAVERIKS"
     gene            complement(4820..7126)
                     /gene="topA"
                     /locus_tag="PK288_08900"
     CDS             complement(4820..7126)
                     /gene="topA"
                     /locus_tag="PK288_08900"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPM19081.1"
                     /translation="MIENLVIVESPAKAKTLEKFLGKDFRVVSSFGHIRDLAKKNLGI
                     DIVNNFSPEYEIPKDKVKIVNDLRKAAGESRIIWLASDEDREGEAIAWHLASVLNLDP
                     ASTRRIVFHEITKDAISNAVLNPRQIDMNLVNSQQARRILDRIVGFEISPVLWKKVQP
                     ALSAGRVQSVAVRLIVEREREIISFKTESAFRVTGLFHPETAEVQENNLIRAEASKKF
                     RDEKDAEAFLKHCIPAKFRVSGITVKPGTRSPAPPFTTSTLQQEAYRKLGFSVAQTMT
                     VAQKLYEAGKITYMRTDSTNLSRLALAKSKEVIVAEYGEKYSKTRQFKTRSKTAQEAH
                     EAIRPAYPEAQTITGSQNEKKLYELIWKRTIASQMSDAEIERTTILIGMNNSPVTFTA
                     TGEVIKFDGFLRLYSESTDTENGDDEKHILPRVEKDMNLLYNTITATQKFTSPLPRYT
                     EASLVKKLEELGIGRPSTYAPTISTIQNRGYVIRGDRPGEKRQIKVLTLSGDRLTSSI
                     RSEAAGKEKSKLFPQDIGMIVNDFLVENFPDIVDYNFTAGIEEQFDKIAEGRLKWTGM
                     LDKFYPVFHKKVTDTLEKKDRKKGIRVLGNHPETGEKVSVKMGRFGPVAQIGDSDTGE
                     KPRFASLLKNQLLETITLEEALNLFRLPRSLGIHEGDEMIAGTGRFGPFIRYRNKFYS
                     LKKGVDDPYTVTLERGVEIIREKNESDSKKVLKDFGDVLLLNGRYGPYLSRDRKNYRL
                     PKGSDPEKMTREDCIRIIEETEKKQKRT"
     gene            7245..>8902
                     /locus_tag="PK288_08905"
     CDS             7245..>8902
                     /locus_tag="PK288_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPM19082.1"
                     /translation="MKSTNPDRKYIVWFWCLFTVPFIVIFLLFLLISRGKLGPMPSFR
                     ELENPEYYLAAEVFSEDGVLLGKISIENRTWTEYKDLSPYLVDALIATEDIRYYRHSG
                     IDVRGLGRTIVRTILLGQNTGGGSTITQQLAKQLYPRDTAKISAFVRNIRLGVTKFKE
                     WQTAIKLERSYTKEEIIAMYLNKFDFLYNAIGIRSAARVYFNTTPDSLNLQEAAVLVG
                     MLKNSARYNPVRNYDLMVKRRNVVLSQLAKYGYINPAIIDSVKQLPIETDFHLEDHNT
                     GLATYLREYLRNTMRRPEPDINRYERESSYRDALWEWENNPLYGWCNKNRKPDGSNYD
                     IYRDGLKIYTTINSRMQLYAEEALREHLSKDVQPGFYRRAKSFRNPPYSNDISKKEVE
                     ELIMRSLKESDRYYVMKARGVPEDSIMLAFNTPVKMRVFSWKGERDTVMTPYDSVRYY
                     KYFIRSAFLVEDPHTGYVKAYVGGPDFRYFKWDAVTQQRKQVGSTIKPFLYTIAMQNG
                     YSPCYEVENIPRSFDIPGDSVPWIPRSSGPKEYHGKMVTLKWGLA"
BASE COUNT         2283 a         1979 c         1966 g         2674 t
ORIGIN      
        1 ttgacacgtt aagtgcaagc atggcggtaa gaatcagata catcataccg atcatttttt
       61 gccgtggaga cagcctctcg tgagccattt ttcaggttgt tttatttttt acttcaagtg
      121 taattatttg acgttcattg cagcaagcat gttcccgtaa acattgttga gtgctgtcag
      181 attgtcattt aattttgcaa tttgctcgcg gtatctgctt gcatcgcccg cggaatcact
      241 aagatcctta attaatccct ggatgccttt ctgaaggcgt tcggattctt ttatatgttc
      301 atcggctccc ttgcgctgga gttcataaac tgcgttaagt gccgaaaggt ttttattaag
      361 tgtttcaagc tgctgatgat agttttttcc tcctgtatca atgaaagata cagtttcagc
      421 catttttcca taaacaagct gggtgtcatt tattgttttg gaagctgcct ggtaggtttc
      481 cgataatctg ttaagcgatt cgttggctgc atgtatggta ttcatatatt ttgcggatgc
      541 agccgaaata tcacccatta cattaatgtt tgatgttgct tcgcttagct tttgcattcc
      601 gacgccgagc ttctggaaaa gttcaggtgt gatttctgct ttttccagca tttcgtcgaa
      661 ctttgaaagt gcgactgaac cgccgccacc gccgtaaccg ccaccgccgt aaccgccacc
      721 ggagtatccg cctccggata tacctcctcc aactgcatat ccgccaccgg catgtccgcc
      781 ctgatatttg gaatcgcggg cacccaatgc acccggttca tcgttaggaa tgccggccag
      841 ttcaggatat accagtgtcc agtcaacctc ttcatgaagc ggttcaaatg ctgaaaagaa
      901 gaatattatt gattctgtgc aaaggcctac ggtaagcatc aagcctgcca gtggccagtg
      961 ctgtattttg aagagagctc cgatgattac gatagaagca cctaaaccgt aaagcttggc
     1021 catgaaattc ttccatccgc tgctttgtac taattctgct aagctcatta tcagttgttt
     1081 ttaagatttt attttacttg atattctctg ctttgaataa tttttttaat gattcacccc
     1141 aatataggat cttacattgc gaaatcctat gtaagacttt gttgaatcct gatattcata
     1201 atcacgcgtg cttgtctgta aaaagtatgc tatatccttc catgaccctc ccctgattac
     1261 ctttcgcttc ataacaggag gatcctgcgg cgttgcattg tattcatagt ttggattgag
     1321 gtcgtgctgg aaaatatatg ctgagggatg atatgcgcag caggtccatt cggcaacatt
     1381 gcctgacata tcaaacaatc cgtattcatt tggttcatag gaaccgactt ttgaggtata
     1441 tacagttccg tcgtcaatat aattaccgcg taagggtttg aagttggcaa gaaaacagcc
     1501 ctggtaattc cgggtgtatg gcccgcccca tgggtacatt gaaagatcga gacctcctct
     1561 tgcagcatat tcccattctg tttcaagcgg aagcctgtag gcctgtacat cggcaatacc
     1621 tttcttcctg agggcagtat tcatgtagtc ggttctccat ctgctgaaag ctgttgcctg
     1681 aacccatgat acaccaacca caggatagtc atcaaatgaa ggatgccaga aataaagatc
     1741 ggcctggggg tcattgaatg agtatgtaaa atcccttatc cagcaaagcg tatcgggata
     1801 aatgtttatc acattgcgtt ttatgaaaga ggaacggtct ttcacttctg tcctggaacc
     1861 atcaggattg gttacatagc cctcatattt tccggtattg tttgcataat cgtgaacgta
     1921 ccttgccttt gcagcctgct gataatcaac ccagaaatag gaatagttca gttttctgat
     1981 atcaatctgc tttttgctgt tgaacctttc ctcaggagga taatacatct cctcgagaac
     2041 tgctgcagta gtctcttccc tcggatcaat cctttcattc cagttgatca cgtttggttc
     2101 aatgacgttg ccttccctgt caaccatgaa ataattcctg tttggaaccc ctgcattgcc
     2161 cagatttgta cggagaatgg aatcgcggac ataataaaca aactgacggt atttgttgtt
     2221 tgttatttca gtctgatcca tccaaaatgc atcaaccgtt acggtttttg agattgtgtt
     2281 cgcggcgaag acggcatcct ggtcgcttgg ccccatctcg aagcttcctg ctggaataaa
     2341 tgccatctca aaaggctcag gctcaaaaaa gttctttctc cctgcaatgc ccgtcagctc
     2401 tccggtattt gatgagccgc agccgcttaa cactactatt gtaatcagag ctgaaaaaat
     2461 tacctttttc atattgctat aggattttat gcaagtacgt ttaattcaaa aatttattat
     2521 aaaaacctga tacttttata tcgcatggga cttttgccca ggctgatgtc gaagcaataa
     2581 ctgaccataa attcatggga tccgcttgta ttcctgataa tgtccgtgac cggaaaatca
     2641 tacgcatatc ctactcttat tccatttgat aattctattc ccagtattgc tgcaaacgca
     2701 tctccgggtc tgtaagaaac gccgccccat atctttttat tgtatcgtat cagggaagtt
     2761 gctgaaaatt ggagaacttt tccgtcagtc atggtataga ccgaaggcag caattccagg
     2821 gatggacttg gcaattgcag aatataccct gctgtaaaat aataatgcct gcttaaatat
     2881 gtaattcctt tcgagaactt gatgttgggt tggtttatat gtgtaactga taaaccggca
     2941 taatactttt cggctttgta ataaagacct aatcctgcat cgaaggccag actgctttcc
     3001 ttactctcgg ggatcaacgg atcgcctgag ggaggtgtga aaatatcgct ttccggtatt
     3061 ttccagaccg ggtcaagggt catgttgaac attccggcac tgatacctat ccccagggtt
     3121 ccctggccaa gctccatatg atatgagtag ttaagtgcca gactgatatc tctttcaaaa
     3181 ccgattttgt cactcttgac catcaatcct gccccgctat ttatcttgaa cggtgcaatg
     3241 ggtgcactga cattgaaaag ggtggttgcg ggggctccct taaatccaac ccattgctgc
     3301 ctgttaacgg cagttgcaca tgccattccc ggaatgccgg caaatcccgg gttatacgtc
     3361 agggtattga acatataatt gctggtcaga gcatcctgct gggaaaatcc gcagacctga
     3421 aaaactacta aaatcaaaaa tatgaaatac agttttttca attcgcaaaa gaatgaataa
     3481 gcccatttat tgaaccggta aagtaaataa aattctactt tgcaaccaaa tctggctgta
     3541 tttagttata aactatattg tattctgttt caacgggaac atcattgtca gatgctattc
     3601 ctggctgaaa aaataacgat gaaaaaagga ataaatttta tggttcagga tttaatccgt
     3661 tcaactgctt ttacccacct gtcaggcagc tcatttttgt tggctttcag gtaatcttca
     3721 tgggaacagg ctatatagac cttgccggaa ttgatgtcag taagttcaat ccaccatcgt
     3781 tcggtataat tgctcttgac gaaaatcagg tcttcttcaa gatcttttac cctcacatga
     3841 tattttataa agcgtcccga attagtgtca ctcagtgcag gagtctcata ctgtttctgt
     3901 gaaaatcctt caaggaagaa ccacagaatc tgcgcagcga ggcctgttgt ctggcctctg
     3961 acatcaaatt ccggattaac atcgaaaagg ccgaaaactt tgagtctgtc agaaatacct
     4021 gcataccggg caagcaggca tatctcttcg ccataaaatc cattcggtga ggggatcgcg
     4081 gttcccgggg catctgactg ccttaccgag gaaatgtcaa acagggctgc ttcggaatcc
     4141 ctgaaaagag gttcggtcag atagatcgcc tgacgcacat ctccaatcct tacaagctct
     4201 gagttgttcc tggcaaaacg gtttagaacc tgctgatcat tgaggtaagt ctggtatccg
     4261 atattgatga aatgttccat ggtcctgttg tgggcccgtg taatgtcatt gaggtacgaa
     4321 gatgaactaa aggcccctgt ttcgctggtg aaatcgattc ttggatccac ggagatcatt
     4381 gtatatttct ttttcagcac cgacattgac ctgtcaattg ctgttatcag tgcgctgctg
     4441 cccccgatta tgaccggaaa tatgttttcg cgcataaaaa agacaagaat atcggtgagt
     4501 cccgcaatcg tatcattgaa agccatcccc tgtttcatgt tcccaaggtc ggcgattttg
     4561 atttttcccg ggatttttgc caggttgtaa agctgctgtc ttatggcatc cggagctttg
     4621 tctgaaccgg ggtttggtga attccgtcca tcgggtacgc ctataagaac taccttgaat
     4681 ctctcgccag gattgattct gtcgttttcg gtgtggatgg aaatattgtg cggaaaacct
     4741 gcattggaag tcatgttcag gaattccggc tcatcaatgc ttacgggatc gaagtaatct
     4801 ttaaggtcca gcatctggtt caggttcttt tctgtttctt ttcagtttct tctattatcc
     4861 tgatacaatc ttctcttgtc attttttcag gatcactacc cttcggcagg cggtaattct
     4921 tcctgtcgcg ggaaagatac ggaccatatc tgccattaag taaaagcaca tccccgaaat
     4981 ccttcagaac cttcttactg tctgattcgt ttttctccct gataatttcc acacctcttt
     5041 cgagagttac cgtgtatggg tcatcaacgc cttttttcag ggagtagaat ttattcctgt
     5101 agcggataaa gggtccgaac ctgcctgtac cggcgatcat ttcatcacct tcgtgaatgc
     5161 ccagcgacct tggcaggcgg aacaggttaa gagcctcttc aagggttatc gtttcaagaa
     5221 gctgattctt cagaaggctt gcaaacctgg gtttttcacc cgtgtctgaa tcaccaatct
     5281 gggcaacggg tccgaatctt cccattttta ccgatacttt ctcacccgtt tccggatgat
     5341 tgccaaggac gcggatccct tttttacgat cctttttctc cagggtgtcg gtcaccttct
     5401 tatggaatac agggtagaac ttgtcaagca taccggtcca tttcagcctg ccttccgcaa
     5461 tcttgtcgaa ttgttcctct atcccggcgg taaaattata atcgacgata tcgggaaaat
     5521 tttcaacaag aaaatcattt acaatcatac ctatatcctg gggaaacagt tttgattttt
     5581 cttttcccgc tgcctctgaa cggattgatg atgtgagccg gtctccggaa agagtaagta
     5641 cctttatctg gcgtttttct cccggccggt ctccccgtat tacatatcct ctgttctgaa
     5701 tggttgagat tgtaggagca tatgtggaag gacgcccgat tccgagttcc tcaagctttt
     5761 tgacaaggct ggcttcggta tacctgggca ggggggatgt aaatttctgg gtggcggtga
     5821 tagtgttata gagaagattc atgtcttttt ccacccttgg caatatatgt ttttcgtcat
     5881 ccccgttttc agtatcagtt gattcagaat aaagcctgag gaatccgtcg aattttatca
     5941 cctcacctgt ggcagtgaat gtaacaggtg aattgttcat tccgatcagt atggtggtgc
     6001 gttcaatttc ggcatccgac atttgtgatg ctatggtcct tttccagata agttcataca
     6061 gctttttttc attctggctt cctgttattg tctgggcttc gggataagcg ggtcggatag
     6121 cttcatgagc ttcctgtgca gttttcgatc tggtcttgaa ctgcctggtt tttgaatatt
     6181 tctctccgta ttcagcaacg ataacttcct tcgactttgc aagtgccagc ctggaaagat
     6241 tcgtggagtc ggttctcatg taggttattt tccccgcttc atataacttc tgtgcaactg
     6301 tcatggtctg tgcaactgag aagcccagct tcctgtatgc ttcctgctga agcgttgagg
     6361 tggtaaacgg aggtgcgggc gatcgtgtac ccggtttgac ggtgatccct gaaaccctga
     6421 atttggcagg gatacagtgc ttcaggaaag cttctgcatc tttttcatca cggaacttct
     6481 ttgaggcttc ggccctgatg agattgtttt cctgaacctc tgccgtttca ggatggaaca
     6541 agcccgttac ccggaaagca gattccgtct tgaaagatat tatttccctt tccctctcta
     6601 caatcagtct gacagcaacc gactgaaccc ttccggcaga aagggcaggc tggacctttt
     6661 tccacaggac cggtgaaatc tcaaaaccga ctatcctgtc aagaatacgc ctggcctgct
     6721 gggaattgac aagattcatg tcaatctgcc gcggattcag gacggcattg gaaattgcat
     6781 cttttgtaat ttcatggaat acgatcctcc ttgtagaagc cggatccagg ttgagaacgg
     6841 aagccaggtg ccaggcaatc gcctcaccct cccggtcctc atcggaggca agccagataa
     6901 ttctggattc accggcagcc tttcgcagat cattgacaat ctttactttg tctttgggta
     6961 tttcgtattc gggggaaaaa ttgttgacga tatctatccc aaggtttttc tttgcaagat
     7021 cccttatatg accgaaactg gaaacaacac ggaaatcttt tcccaggaat ttctctaatg
     7081 tttttgcttt cgccggcgac tccacaatga ccagattttc tatcattacc ggtatggatt
     7141 tttctgttaa aaacacgaca aagtaaatca tttaaggggc aaacttcaaa atttaccgtc
     7201 aagattcttt tataattgct atttttgcgg caaattaaag ttgaatgaaa agcacaaacc
     7261 ccgaccgaaa atacatagtc tggttctggt gcttgtttac cgtaccgttt attgtaatat
     7321 ttcttttgtt tcttctgatt tccaggggaa agctggggcc tatgccatca ttcagggaac
     7381 ttgagaaccc tgaatattat cttgcggcag aggtcttttc cgaggacggg gttcttttgg
     7441 gcaaaataag cattgaaaac aggacatgga cggaatacaa ggatttgtca ccttatctag
     7501 ttgatgcgct gattgcaacc gaggatataa gatattaccg tcattcgggt attgatgtca
     7561 ggggtcttgg ccgtacaata gtacggacta ttcttctcgg gcagaatacc gggggcggaa
     7621 gcacaattac ccagcagctt gctaagcagc tgtatccgag ggatacggca aaaatatcag
     7681 ctttcgtgag aaatatcagg cttggtgtga caaagttcaa ggaatggcag actgcaatta
     7741 agcttgagcg cagctataca aaggaggaga tcatcgcaat gtatctgaat aagttcgact
     7801 ttttgtataa tgctatcggc atcagatcgg ctgcaagggt atacttcaac accacacccg
     7861 attccctgaa ccttcaggag gctgccgttc ttgtcgggat gttgaaaaat tctgcaagat
     7921 ataatcctgt caggaattat gatctgatgg tgaaacggag gaacgtggta ctttcccagc
     7981 tggctaagta tggctatata aatcctgcaa taatagactc ggtaaaacag ctgcccattg
     8041 aaaccgattt ccatcttgag gatcataata ccgggcttgc aacatatctg agggaatacc
     8101 tgaggaatac catgcgcaga cctgagccgg acattaacag atatgagcgt gagtcttcgt
     8161 accgtgatgc gctgtgggag tgggagaaca atccgttata tggctggtgc aataaaaacc
     8221 gcaaacccga cggctcaaat tatgatatat acagggatgg cctcaaaatc tatactacaa
     8281 taaattcccg gatgcagctg tatgccgaag aagcattgcg ggaacatctg tcgaaggatg
     8341 tgcagcccgg attttacagg agagccaaat cattcaggaa tccgccctat tcgaacgaca
     8401 tttcaaaaaa ggaagttgaa gaacttataa tgaggtctct gaaggagagc gacagatatt
     8461 atgtgatgaa agcacgcggc gttccggaag attctataat gctggcattt aatactcctg
     8521 taaaaatgag ggtgttttca tggaaggggg aaagggatac ggtaatgacc ccatatgatt
     8581 cggtccgtta ttataaatat ttcatccggt ccgcatttct ggttgaagat cctcataccg
     8641 gttatgtaaa agcctatgtc ggaggacctg atttcagata ttttaaatgg gatgcggtaa
     8701 cacagcagag aaagcaggtt ggaagtacaa ttaaaccttt tttgtacacc attgcgatgc
     8761 aaaatggtta ttccccatgc tatgaggtgg aaaatattcc ccgttccttc gatatcccgg
     8821 gcgattctgt tccctggatc ccaagaagct cgggaccaaa ggaatatcat ggcaagatgg
     8881 ttacacttaa atggggactt gc
//
LOCUS       DAOOYG010000106         8864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_8705_length_8864_cov_4.805199, whole genome shotgun sequence.
ACCESSION   DAOOYG010000106 DAOOYG010000000
VERSION     DAOOYG010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8705_length_8864_cov_4.805199"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="PK288_08910"
     CDS             <1..1264
                     /locus_tag="PK288_08910"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPM19083.1"
                     /translation="GGKLVDSVLTFIELRQLFDEFEIQERTVKMSEFDPPYGNWGALY
                     PLPAGIVQAGGIKRDFFTSNVITAAGKEEVFEALNDFGQYIDTIHHHFNLFFCHGCML
                     GPGMERHSERFRRRALVRLYAEKRVGLLDKAQWQKDMERWSKLDFSRSFNPNDQRIPE
                     PPAEAINEVLKIIGKDNPDEELNCGACGYQSCREFASTVAKGLAVPEMCHTFNLRNKQ
                     EYIETLRQTNRKLAETKKALKDSQELAMREKEMAQSASDMMHNMLEKLPTGVVIVDNN
                     LKILHSNQSFINIIGEDAKSIADIIPGLVGADLKTLMPFNIYNMFTFVLKEDEPVVSK
                     DFRFEDNMLNISIFPIRKNKICGAIIRDLFSPEVQGEEVTNRVSEVIEKNLEMVQKIG
                     FLLGEGASETEQMLNSIIESYKKRRVTR"
     gene            1285..2469
                     /locus_tag="PK288_08915"
     CDS             1285..2469
                     /locus_tag="PK288_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPM19084.1"
                     /translation="MYKDFYIEVNSQQRNHDGERISGDVFLYRYIKEEDRVIAVLSDG
                     MGHGVKANILATLTATMAMNFTIEHKEVDTIAEIIMNTLPVCSERKISYSTFTIIDIE
                     SSGKTNILEYDNPSTIILKGSDVFDPSWKKVVLEKGKNAGKVLKTCSFHPSKEDRIIF
                     CSDGVAQSGMGSEMFPFGWGRDEVASYAASLVRGEASISAKTLSGKIVTMAHKNDSYK
                     AKDDISCATIYFRDPRKLLICSGPPYEKDKDRELAEKVKNYNGKVILCGGTTADIVAR
                     ELNKTIVDELIFEDPDLPPESFIDGIDLVTEGILTLQKVNEILKSYNNSVRLGKGPAD
                     KIVRLVMESDEIHFIIGTRINIAHQDPTLPVELEIRRTVIKRIARLLEEKWLKKVSFE
                     YI"
     gene            complement(2482..2916)
                     /locus_tag="PK288_08920"
     CDS             complement(2482..2916)
                     /locus_tag="PK288_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPM19085.1"
                     /translation="MTEVRFYDPSFCPAGGLTYSVIVSRYRGRWLFVRHHGRTTWEIP
                     GGHIENNETSDDAARRELNEETGATEFELECVSTYSVTTDGETGYGRLYFAEIFSLGP
                     IQDVSEISEIKTDIFRPGENTYPYIQSCMFERIQAYLKSRKE"
     gene            2994..4175
                     /locus_tag="PK288_08925"
     CDS             2994..4175
                     /locus_tag="PK288_08925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPM19086.1"
                     /translation="MIILIFLIIIEILTFQVIRQHLYDRSWLRYYFMVTFNTLVSVWF
                     WVIWFRLANFRGIYDEPVNVWLLMNLNGLTAAVVIPRFILVIFHFSGVLARRQTGGHI
                     RSLTNTGLIIAAVIFFTALTGSLFERFNFRKENISVDVRGLHPDLEGLKIVQISDLHL
                     SSFYHHAGQLAGVMKEIDEIGPDLLINTGDFVTIGWREFAGFDTILRTAGGRYGAYAV
                     MGNHDFGTYDPYFTEADRQNNVMLMNKLISSSGYKVLNDESAMIEIGGAKVSLSGITT
                     MGRFPEIISGDLSEALKGSDSADLKILLAHDPNFWEKSVTGKTDIDITFSGHTHGMQF
                     GIVTKRFAWSPASWFYPRWNGLYRKDNQVLIVSRGLGVLGFPFRIGMPPDIVVITLNS
                     G"
     gene            4343..5062
                     /locus_tag="PK288_08930"
     CDS             4343..5062
                     /locus_tag="PK288_08930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPM19087.1"
                     /translation="MKTAPLKDHIMSARYFDYRIRYSRRKSLSIIVSPDKGVIVRAPH
                     RIQAGIIEKFVSEKSQWIIKCLESFSSMQRIDNNNGYHDGDRVLLHGEFYTLRLVSSD
                     RFNVRLRDKRIIEIEYLQKNDPMLIREVLESWFRHIAVRELTARFRELLEHYSAFGFK
                     PAGFSVRKMKKRWGSCSSKGMIGISYDLIRLGREFSDYVIAHELCHLIHHNHGTGYYK
                     LLSEVYPQWREKRAELKKYLR"
     gene            complement(5101..6501)
                     /locus_tag="PK288_08935"
     CDS             complement(5101..6501)
                     /locus_tag="PK288_08935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPM19088.1"
                     /translation="MKLKFLGAAREVTGSKVLLEPGSGKRILFDCGMFQGKGLETDAM
                     NRDLGFDPVEIDHVILTHAHIDHSGLIPYLYKLGFRGSVICTNATRDLCSIMLADTAF
                     IQEHDTRTFNKKRARKGLPLVTPLFTREDADDCMKLFIGVPHGMKFRIDDNLKVRFTN
                     TGHMLGSGVANLEVRENGTIRRVACTGDIGRPSSRILKPPEAFPQADILITESTYGDR
                     LHKDYASADDELLRTVVHTCVDKGGKLIIPSFSVGRTQELVYSFNKFFNEDRLPRVEI
                     FVDSPLAVNATTIFSMHPECFNRETIALMQTDPDPFGFNRLNYIVRREDSKKLNDYRK
                     PCIIISASGMMEAGRIKHHLANNISDPRNTVLIVGYCSPATLGARIAGGAREVSIHGT
                     LYSVNAEIKKIDAFSGHGDYREMISFLECQDKKALQDMFLVHGEYETQLKYSGELKKA
                     GFNNIHIPSMRQEYTL"
     gene            complement(6577..7893)
                     /locus_tag="PK288_08940"
     CDS             complement(6577..7893)
                     /locus_tag="PK288_08940"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPM19089.1"
                     /translation="MNNRDLARKIFMEGVKSVLPEKLITGSLSLQGSILWAGDHQVDL
                     RGKRNIYVIGAGKASGAMGHYVETVLGDRISGGHIVVKYGYACKLRKITVTEAGHPVP
                     DSNSFMAADAIAGIARQAEKDDLVICLISGGGSALLTDLPGGLLPEEIHIVNNLLTRC
                     GATIREINTVRKHLSGIKGGQLAAMVYPAALITLIISDVIGDAPDVIASGPTVPDAST
                     YDDAFQVIEKYGLASDLTAGIMKYLNDGRIGLRSETPKPGDPLFSDQVIFLCGTNRIA
                     LEAAKNYAELAGLESYIADDSLQGDVELAAEVIFNAAADCRANRTPGKPACLIFGGET
                     TVRVSGEGLGGRNQHLALSLAMRLRDKPGITILAAGTDGTDGPTDVAGAVVDSETIVQ
                     AEKQKIDPEKYLAAFDSYNFFKKTGGHIRTGPTFTNVMDMAIVLVE"
     gene            complement(8039..>8864)
                     /locus_tag="PK288_08945"
     CDS             complement(8039..>8864)
                     /locus_tag="PK288_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19090.1"
                     /translation="IDANIGYLVMTGTLFAIPGALAAYFWSRFFSRAGRPIPTGVPAG
                     NENKSVPGLPPVFLSFLPIVVPLLLITIKSLTAIGDKTGQSALSRILAFPGEPVIALF
                     IGVLLSLLLVKKRNIESLNALFSEAVVKAGPILIITAAGGMFGLVIKSTGVGEAMGEI
                     LSGSKAGILVPFLMAAIMKTAQGSSTVAIITAASFVSPMLPGLGLDSEWGRLFAMLAM
                     GSGSMVVSHANDSYFWVVTNFGEISVNDTLKVYTTSTLVMGIVVFLCVWLSSAFLL"
BASE COUNT         2416 a         2120 c         2117 g         2211 t
ORIGIN      
        1 gggaggtaag cttgttgaca gtgtgctgac tttcattgaa ctgaggcagc tgtttgatga
       61 gtttgaaata caggaaagaa ccgtaaaaat gtcggaattc gatcctcctt acggcaactg
      121 gggcgcattg tatcccctgc ctgcaggaat agttcaggcc gggggaataa aacgcgattt
      181 ctttaccagc aatgtgatta ccgcagcagg caaggaagag gtcttcgaag ctctgaatga
      241 ctttggccag tatatcgata ccattcatca ccacttcaac ctgttcttct gccacgggtg
      301 catgcttggt ccggggatgg agcgccattc ggagaggttc aggagacgag ccctggtaag
      361 gctttatgct gaaaaaaggg tcgggttgct ggataaggca cagtggcaga aggatatgga
      421 aagatggtcg aaactcgact tttccagatc attcaatccc aacgatcaaa ggatccccga
      481 acctcccgcc gaagcaataa acgaggtcct gaaaatcatc ggcaaggata accccgatga
      541 agaattgaat tgcggggcat gcggttacca gtcgtgccgg gaatttgcat caaccgtggc
      601 aaaaggactg gcggttccgg aaatgtgcca tactttcaac ctccgcaaca aacaggaata
      661 cattgaaacc ctgaggcaga ccaacaggaa gcttgccgag acaaaaaaag cccttaagga
      721 ttcgcaagag cttgcaatga gggagaagga aatggcacaa agcgcttcgg acatgatgca
      781 caacatgctt gaaaaacttc ccacaggtgt tgtgattgta gacaataacc tgaaaatcct
      841 tcactcaaac cagagtttca tcaacattat cggcgaagat gcgaaatcca ttgccgatat
      901 tattcccggc ctggtgggtg ctgaccttaa aacactgatg cccttcaata tatataatat
      961 gttcactttc gtcctcaagg aagacgaacc ggtggtaagc aaggatttca ggtttgagga
     1021 caatatgctg aatatttcca ttttccccat caggaagaat aaaatatgcg gcgccataat
     1081 tcgcgacctg ttctctcccg aggtccaggg agaggaagtc acaaacaggg tcagtgaagt
     1141 gattgaaaag aacctggaga tggtgcagaa aatcgggttc ctgctgggcg aaggagcctc
     1201 agaaaccgaa cagatgctta actcaatcat cgagtcatat aaaaaaagaa gggtgaccag
     1261 gtaacatttc cacactaaca gagcttgtac aaggattttt acatagaagt caacagccag
     1321 cagagaaatc acgatgggga acggatctcc ggggatgttt ttctttacag gtatatcaag
     1381 gaagaagaca gggttattgc agtactgtct gacggtatgg ggcatggtgt aaaagccaac
     1441 attcttgcca cccttaccgc aaccatggct atgaacttca caatagaaca caaggaggtt
     1501 gacaccatag ccgaaataat aatgaacacg ctgcccgtct gcagtgaaag gaaaataagc
     1561 tattcaacct ttaccatcat cgatattgaa agcagcggaa agacaaatat cctggaatat
     1621 gacaatcctt caacaataat actgaagggt tccgatgtct ttgacccctc atggaagaaa
     1681 gtggttctgg aaaagggcaa gaatgccggc aaggtcctca agacatgttc atttcatccg
     1741 tcaaaggaag acaggatcat cttttgttcc gacggtgtgg cccagagcgg tatgggaagc
     1801 gaaatgttcc cgttcggctg gggacgtgat gaggttgcat cctatgctgc atccctggtc
     1861 aggggcgaag catcaatttc tgcaaaaaca ctctcgggga agattgttac catggcacac
     1921 aagaacgaca gttacaaggc caaggacgac ataagctgtg ccaccattta tttccgcgat
     1981 cccaggaagc tgcttatctg ttcaggtccg ccctatgaga aggacaaaga ccgtgaactt
     2041 gccgaaaaag tgaaaaacta caacggcaag gtaatcctgt gcggaggtac aactgccgat
     2101 atagtggcca gggaacttaa caagaccata gtcgatgaac tgatctttga agatcccgat
     2161 cttccgccgg aaagttttat cgatggcatt gaccttgtaa cggaaggtat cctgacgctt
     2221 cagaaggtga atgagatcct aaaatcatac aataacagcg taaggctcgg gaaggggccg
     2281 gccgacaaga tcgttcgact agttatggaa agtgatgaga tccatttcat tatcggaaca
     2341 aggatcaaca ttgcacacca ggatcccacc ctgccggttg agcttgagat aaggagaacg
     2401 gtcataaaac ggattgccag gttacttgag gaaaaatggc tcaaaaaagt cagcttcgaa
     2461 tatatctgaa tcttaacctc tttactcttt tctgctttta aggtaagcct gtatcctttc
     2521 aaacatgcat gactgtatat aaggataggt attttccccg ggccggaata tatctgtctt
     2581 tatttcactt atctccgaaa catcctggat cgggccgagg ctgaaaatct cggcaaaata
     2641 aagcctccca taacctgttt ctccatccgt ggtcaccgaa taggttgaaa cacattccag
     2701 ttcaaattcc gttgccccgg tctcttcatt aagctcacgg cgggcagcat catcggaggt
     2761 ttcattattt tcgatatgcc cccccggtat ctcccatgtg gttcgtccat gatgacggac
     2821 aaacaaccat ctgccccggt atcttgacac aataaccgaa tacgtcaggc cgccggccgg
     2881 acaaaaggag gggtcataaa accttacttc agtcatcttt tttaaaccgg cctaaaagta
     2941 attaaaatga ctgataactt tattgagtat ttttaccctt aaaaacatcc cggatgataa
     3001 tcctgatttt tcttataatc atcgaaatcc ttacattcca ggttataaga cagcatctgt
     3061 acgacaggtc atggctgagg tactacttta tggttacttt caacaccctt gtaagtgtgt
     3121 ggttctgggt gatctggttc aggctggcaa acttcagggg tatatatgat gagcctgtca
     3181 atgtgtggct tttaatgaac ctcaacggcc tgacggctgc ggttgtgatt ccaagattca
     3241 tcctggtaat atttcacttc tcgggagtat tggcaagaag gcaaaccgga ggtcacatca
     3301 gatcccttac aaacaccggg ctgataattg ctgcagtgat atttttcact gctcttaccg
     3361 gttcgctatt cgaaagattc aatttcagga aggaaaatat ctccgttgat gtaaggggat
     3421 tgcaccctga ccttgagggg ctgaagattg tccagatttc ggatctgcac ctctcgtcat
     3481 tttaccatca tgccggacag ctggcaggtg tgatgaagga aatagacgaa attgggcctg
     3541 acctgctgat caatacaggt gattttgtta caatcggctg gcgtgaattt gccggtttcg
     3601 acacgattct cagaaccgcc gggggcaggt acggagccta cgcggtgatg ggaaaccacg
     3661 atttcggcac atatgacccg tatttcacag aagccgacag gcagaacaat gttatgctga
     3721 tgaataaact gatatcctca tcgggatata aggtgcttaa cgatgaatcg gcgatgattg
     3781 aaataggcgg tgcaaaggtc agtctgtcgg gaataaccac catgggccgt ttcccggaaa
     3841 taatttcggg cgatctgagt gaagccctaa agggaagcga cagcgccgac cttaaaatac
     3901 tcctcgccca tgatccgaat ttctgggaga agtccgtcac aggtaaaaca gacatcgata
     3961 taactttttc ggggcacact cacggcatgc agttcgggat tgtcacaaaa cgatttgcct
     4021 ggagtcctgc aagctggttc tatcccagat ggaacgggct gtaccggaaa gataaccagg
     4081 tccttattgt cagcaggggg ctgggggtcc tgggatttcc attcaggatc ggaatgcccc
     4141 ccgacatagt ggtaatcaca ctgaactccg gatgaagcaa tagactgaaa actaagatgt
     4201 cagaataaaa ccatttaaac gcaacagctt cggggcttac tgcggtatct gtgcagggaa
     4261 attcaccgtt gtaagttaca ggctcattgc aaccgccccg ctgatttaac ggttaaaaaa
     4321 cctgcagcat agaaacccgg aaatgaaaac cgcaccactg aaagaccata taatgtcagc
     4381 aaggtatttt gattacagga tcaggtattc acggagaaaa tccctctcaa tcattgtatc
     4441 gcccgacaag ggagtcattg taagggcccc tcacaggatt caagccggaa tcattgaaaa
     4501 attcgtaagc gaaaaatctc aatggataat aaaatgtctt gaaagtttca gttcaatgca
     4561 gcggatcgat aacaataacg gataccatga tggtgaccgg gtcttgctcc atggagaatt
     4621 ctacacactc aggctggtgt cttccgacag gtttaatgta cggctcaggg ataaaaggat
     4681 cattgaaatt gaatacctcc aaaaaaatga tccaatgctg atcagggaag tccttgaatc
     4741 atggttcagg catattgcag tcagggagct gactgccagg ttcagggaat tgcttgaaca
     4801 ctattctgcc tttggattca aaccggcagg gttttccgtc agaaaaatga agaaaagatg
     4861 gggcagctgc tcatcgaaag gcatgatcgg cataagctat gacctgataa ggctcggcag
     4921 ggaattcagc gattatgtca ttgcacatga gttgtgccat ctcatccatc ataaccatgg
     4981 tacaggttat tacaaactcc tttccgaggt atatccccag tggagggaaa agagggcgga
     5041 gctgaaaaag tacctgagat aaaaggcgga tgcctgtcag gtcatcatcc cgatatgatc
     5101 tcaaagagtg tactcctgcc tcattgaagg tatatggata ttattgaatc cggccttttt
     5161 gagttccccg gaatatttta actgggtttc atattcgccg tgcacaagga acatatcctg
     5221 caaggccttt ttatcctggc attccaggaa actgatcatt tcccggtaat caccgtgtcc
     5281 gctgaatgca tcgatctttt ttatttcagc atttacactg tataaggttc cgtgtatgga
     5341 tacttccctg gcaccgccgg cgatacgagc cccaagggtg gcaggtgagc agtatcccac
     5401 gataaggacc gtattccttg gatctgatat gttattggca agatgatgct ttatccggcc
     5461 cgcttccatc attcccgatg cggagatgat gatgcagggc ttccggtaat cattgagctt
     5521 tttcgaatct tcgcggcgta ctatataatt gagccggtta aatccgaacg gatcgggatc
     5581 ggtctgcatt aatgcaatgg tctccctgtt gaaacactcc ggatgcatgc tgaatattgt
     5641 tgttgcgtta accgccaggg ggctgtcaac aaatatctcc accctgggca acctgtcctc
     5701 attgaagaat ttgttgaatg aatataccag ttcctgggtt ctgcccacac taaaggaagg
     5761 gattatcagc ttgcccccct tgtcaacgca tgtatgtacc actgtccgga gcaattcatc
     5821 gtcggctgag gcataatcct tatgaagcct gtcgccatat gtcgactcgg tgatgagaat
     5881 atcagcctgc gggaaagcct ccggcggttt aaggatccgg cttgagggcc tgccgatgtc
     5941 gcccgtgcag gcaaccctgc ggatcgttcc gttttcacgc acttccaggt tcgccacacc
     6001 gctgcccagc atatgaccgg tatttgtgaa tctgaccttc aggttatcat ctatcctgaa
     6061 tttcatgccg tgcggaactc caatgaacag tttcatgcaa tcatcggcat cttcccgggt
     6121 gaacaacgga gtgacaagag gcaggccctt cctggccctt ttcttgttga aggtcctggt
     6181 atcatgctcc tggatgaacg ctgtatcagc cagcatgatt gaacacagat cccttgttgc
     6241 attggtgcag ataacggaac ctctgaaacc tagtttgtac agatagggaa ttagcccgga
     6301 atgatcaata tgtgcatggg taagaattac atgatctatt tcaacaggat caaatccgag
     6361 gtccctgttc attgcgtccg tctcaaggcc ttttccctga aacatgccgc agtcaaagag
     6421 aatcctcttt cccgatccgg gttcaagaag tactttgctg cccgtaacct ccctggcagc
     6481 gcccaggaac ttaagtttca tgtttatttt atttacgttt aaccggtgtc agtaactcca
     6541 tcgatatttc ccgggaggga aggcaagatt cccggttcat tcaacaagaa caatcgccat
     6601 gtccataaca tttgtaaaag taggacctgt tctaatatgt cctccggttt ttttgaaaaa
     6661 attataggaa tcaaacgcgg ccaggtattt ttcaggatca atcttctgct tttctgcctg
     6721 cacaatggtt tccgaatcca caactgctcc tgccacatcc gttggtccgt cggtgccgtc
     6781 cgttcctgct gccaggatcg taatgcccgg tttgtcacgc aaacgcatgg caagggaaag
     6841 ggccagatgc tggttccggc cgccaaggcc ttccccgctt accctgacag tggtttcacc
     6901 cccgaaaatc aggcaggcgg gttttcccgg cgtcctgttc gccctgcaat ctgctgcagc
     6961 attgaaaatc acttctgcag ccaattccac atccccctgc aatgaatcat cggctatata
     7021 agactccagt ccggcaagtt ccgcataatt ttttgcggcc tcaagcgcaa tcctgttcgt
     7081 tccgcaaaga aaaatcacct ggtccgaaaa aagaggatct ccgggtttgg gtgtctcaga
     7141 ccgcaatcca atcctgccat cgttgaggta tttcatgatg cctgctgtca gatctgaggc
     7201 aaggccatat ttttcaatca cttgaaaagc atcatcatag gttgaagcat caggaaccgt
     7261 cggtcctgag gcaatcacat ccggtgcatc tccgattaca tctgagatga tcagggttat
     7321 cagagcggcc ggatacacca tcgcggccag ctgtcctccc ttaatccccg acaagtgctt
     7381 tcttacagtg tttatctccc ggatggtggc tccgcacctt gtgagcagat tgttaactat
     7441 gtggatctct tcaggaagaa gtcccccggg aaggtccgtt agcagggcag aacctccccc
     7501 tgaaataagg cagatcacga ggtcatcttt ttcagcctga cgggcaatgc cggcaattgc
     7561 atcggctgcc atgaaactgt ttgaatcagg gacaggatgg ccggcttccg ttacagttat
     7621 cttcctgagc ttgcaggcat atccgtattt gacaacaata tgccctccgg aaatgcgatc
     7681 cccgagaacg gtttccacgt aatgccccat tgccccgctt gccttgcctg caccgattac
     7741 ataaatgttt cttttgcccc ggaggtcaac ctgatggtct cctgcccata aaattgagcc
     7801 ctgaagactc aggcttccgg taataagttt ttcaggaaga acgcttttca cgccttccat
     7861 gaagattttc ctggctaaat ccctgttatt catgttcagg tgtatcaaag gttattgcat
     7921 cagtccgttt tttctgatta tcctcctggt tacggtgtct ccggaacact ccggctcatt
     7981 ctgccgtcga tcggcgagta tcatttgtaa ttggctcttt tccatcagcc gcagcaactt
     8041 aaagaagaaa ggcagaagaa agccatacac aaaggaaaac cacgataccc atgaccaggg
     8101 tagatgttgt ataaacctta agcgtgtcat ttacactgat ctctccgaag tttgtcacta
     8161 cccagaaata ggaatcattg gcatgagata cgaccatgga gcctgatccc attgcaagca
     8221 tggcaaaaag cctgccccat tcgctgtcaa gtcccagccc cggcagcatc ggagaaacaa
     8281 atgatgcggc agtaatgata gcaacagttg atgatccctg ggcagttttc atgatcgctg
     8341 ccattaagaa cggaacaagg attcctgctt tgctgccgga caggatttcc cccattgcct
     8401 ctcccacccc ggtggatttg attacaagtc cgaacatgcc tcccgcagcc gtaatgatca
     8461 gtatgggacc cgccttgaca acagcttctg agaatagggc attcaatgat tcaatattcc
     8521 tctttttaac caggagcaac gacaggagaa caccgatgaa cagggctatt acgggctcac
     8581 cgggaaaagc caggatgcgg gacaaggcac tttggcctgt cttgtcgcca atagcagtca
     8641 gcgatttgat tgtgatcaac agcagcggga caacaatcgg gagaaacgac agaaacaccg
     8701 gaggcaggcc tggcactgac ttgttctcat ttccggctgg tacaccagtt ggaatcggtc
     8761 ggccagcccg tgaaaaaaac ctgctccaga aataggctgc caatgcaccg ggaattgcaa
     8821 aaagagttcc cgtcatcaca aggtatccga tgttggcatc gata
//
LOCUS       DAOOYG010000107         8854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_8714_length_8854_cov_6.986930, whole genome shotgun sequence.
ACCESSION   DAOOYG010000107 DAOOYG010000000
VERSION     DAOOYG010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8854
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8714_length_8854_cov_6.986930"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PK288_08950"
     CDS             complement(<1..362)
                     /locus_tag="PK288_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPM19091.1"
                     /translation="MEDFTMALVKFTNRYPSFFDRFFDSELFDWTSRNYSETNTTIPS
                     VNIKESDDDFEVEMAAPGFTKNDFRVELNHDILTVSSEKKVENETREGQEFTKREFSY
                     QSFCRSFTLPHTVDNQKIS"
     gene            complement(596..1528)
                     /gene="metA"
                     /locus_tag="PK288_08955"
     CDS             complement(596..1528)
                     /gene="metA"
                     /locus_tag="PK288_08955"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HPM19092.1"
                     /translation="MPLNISDNLPAVRILEDENIFVMKETRALHQDIRPLQILIFNLM
                     PVKITTETQILRLLSNTPLQVEITLIYTKMHLSKNTPREHLDAFYKTFDDVSRHKYDG
                     MIITGAPIEHLEFEQVDYWEEMTRIMKWADHHVTSTMFICWASQAGLYYHYGIPKYMV
                     GEKIFGVFNHRIYNKTNILMRGFDDEFLAPHSRYTEVRREDIEKVSELEILADSDEAG
                     IYLVASRDLKRVFVTGHSEYDPLTLKSEYDRDIAKGLDIRVPKNYFPHDDPLKQPMVR
                     WRSHANLLFSNWLNYCVYQITPYILENGGKPDTA"
     gene            complement(1878..2315)
                     /locus_tag="PK288_08960"
     CDS             complement(1878..2315)
                     /locus_tag="PK288_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19093.1"
                     /translation="MAREIISAGDIRRLKQEAAASKTDGKGAQPARDSYSDRLFKYIP
                     AELVAGYIFIQGILKNIPQGREALILQWIVFGLFCLLTPLYLRRIQKVFKIQQLLISL
                     LSFVVWCFALGGPFALSGWYDPVYGQILLPVFTLAAAIWEAEK"
     gene            complement(2327..3970)
                     /locus_tag="PK288_08965"
     CDS             complement(2327..3970)
                     /locus_tag="PK288_08965"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPM19094.1"
                     /translation="MYTYTLGGKSGKKYVLHESTDMVALRTRSSRSIKNAVSSPGGRK
                     ALKDFELVLEFPEADISVYRLREPIKDPVAVRDRARMMLKKEPELRFAGKVLVEADGR
                     TLVLYTENIFIKFHDEVRSEICEKILKDNKLRIKQKPDYSKNTYFAGAPENTGLRIFS
                     IAESLLAMEEVELCHPELIRKRGFKGINPKQWHLAGTLINGRRINAGVKADLAHSFST
                     GKNIVIAVIDDGVEIDHPEFRLPGKVVHSRDVVTGSNDPRPRSSYNNHGTACAGVAAA
                     SGMEASGVAPGAMLMPVRLSANLGSAAEAAAFKWAADHGADIISCSWGPEDGSWSDPD
                     DPVHTTMVDLPDSTRLAIDYAVSHGRNGKGCIITFAAGNGNEDCGYDGYAGYEKVMAV
                     AACNDRSRRSIYSDYGKNIWCCFPSNDFGYPPFNHPDPLTPGIFTTDRLGRAGYNPGG
                     NYTDSFGGTSASCPGVAGTAALVLSANPALKWTQVRKIIRETCDKIDISGGEYDSEGH
                     SRFYGYGRINALKAVKLALETGAAGKSGKRRKKSVTVNQ"
     gene            4600..4734
                     /locus_tag="PK288_08970"
     CDS             4600..4734
                     /locus_tag="PK288_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19095.1"
                     /translation="MKIFYQITSKEFGTVLLKKRKIAKALRWWLRRNGLAYTHSFFIS
                     "
     gene            4968..6119
                     /locus_tag="PK288_08975"
     CDS             4968..6119
                     /locus_tag="PK288_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPM19096.1"
                     /translation="MVPVMVMSQARFNGLDMNMGNLYRLSDAKTRSISPENFTGEKGK
                     GGMARLEDKDKPNAANSAHAARDLGQGWKVNPLVRILPGETFTMAEIEGPGAIQHIWM
                     TPAGNWRFSIIRIYWDDEAEPSVECPVGDFFGMGWGQYAPLSSLAVCVNPGSALNCYW
                     VMPFRKKCRITMENVGDQDPMTLFYQIDYTLTDVPADAAYFHAQYRRLNPNVTSDYTI
                     IDGIKGKGHYVGVYMAVGVNNNGWWGEGEIKFFIDGDTRFPTICGTGTEDYFCGSYNF
                     ETRKRNDAGVEVGDYTEFCTPYAGLHQVIRGDGRYRVMQRFGMYRWHIMDPIRFEKDL
                     KVTIQDLGWRQGGRYLAQKSDIASVCYWYQTEPHARFPKIPGWRELEVY"
     gene            6136..7509
                     /locus_tag="PK288_08980"
     CDS             6136..7509
                     /locus_tag="PK288_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM19097.1"
                     /translation="MEQSKNNNSEKKKGLDRRGFISRSSLAAIGAIGTAGLLGSCSGK
                     SRKPELKLPTLLNVAPDGKLLKAGLIGCGGRGTGAAINFLDAGPNLEITALGDVFRDK
                     LDQCREQLKKERNVEIAEEKCFVGFDSFEKVIDSGVDIVLLCTPPHFRPAHVEAAVAA
                     GKHIFMEKPCAVDPVGARRVMVAAKRAADKELSIVSGTIRRVQKDFMETQRRVASGQI
                     GEIVSAHITRNGGSLWVIQRQKGWSDMEYMLRNWANFCWLSGDHIVEQFIHEIDVMNW
                     YLGKNPVKAMAWGGRQRRVTGDQYDFFSVEYIYDNGMHTHCAARQITGCSNLTEQFIV
                     GTEGFANARGMIYNLKGEEVWKYPYPEEGSTDETWKVKDPFVQEHINLVTAIRTGKPY
                     NDAEAQVNSTLITIMGRMSAYTGKDVTWDEVLNSDLYLGPKTYEFGPVPGIPETPPVI
                     GAEPKLG"
     gene            7714..8610
                     /locus_tag="PK288_08985"
     CDS             7714..8610
                     /locus_tag="PK288_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPM19098.1"
                     /translation="MNRRQFAKTTMMASVIASAGTLPVLSGLRSTAAPQLKKGIMWGS
                     NGVGKTIIEKFRSAKAAGFDGIEPMSHLDRNEILKARDATGLSIPSVCGEYHWKYLLS
                     DPDPKVRQNGVEALKVSLEDAKAYGADTVLLVPGRVTETVAYDECWNRSVEEIKKVLP
                     LAEKLNVCIAIENVWNGFLLSPVEAAYYVDQFESPWVKFYFDCGNILTYGYPEQWIRI
                     LGNRIAKIHIKEYSRKIADKQGRSAGFGVKLREGDVNWSAVMDALESIGYSGWTTVEQ
                     PGGDTPEGLKDLCDRLRLIQQS"
     gene            8669..>8854
                     /locus_tag="PK288_08990"
     CDS             8669..>8854
                     /locus_tag="PK288_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19099.1"
                     /translation="MKKISLRPVSWAIVFALLLVSCSGNRQELQKEGHQFNGSYSGKN
                     LDRIAFPIGGIGAGMFCV"
BASE COUNT         2307 a         2065 c         2219 g         2263 t
ORIGIN      
        1 gaaattttct gattgtcgac ggtgtgcggc agcgtgaatg agcggcagaa cgactggtag
       61 ctgaattcac gcttagtgaa ctcctgtcct tccctggtct cgttttcgac cttcttttcg
      121 gaagaaaccg tgagaatatc atggttcagc tccaccctga aatcattttt cgtgaagccc
      181 ggagcagcca tctcaacttc aaaatcatca tcactttcct taatgttcac cgacggaata
      241 gtggtgttgg tttcagagta gttgcggctc gtccagtcaa acaattcgct gtcgaagaaa
      301 cgatcgaaga acgacggata acggtttgtg aattttacaa gtgccatggt gaaatcctcc
      361 attattttat gttaaacatt tttttacttc tgaccatgaa tagtcaaacg atgtaccatt
      421 catggttaag caccgctgag aaggggcaat atgactttaa tcacctggat tccagtatac
      481 tggcaggaaa acaaataaag tgtcaaattg acatacaaat aacaaatgac atgtcagaat
      541 gacatgtcgg aaatttttac atgtgttctg tcaggaaggt caggaaatcc cggaatcagg
      601 ctgtatcagg tttccccccg ttctccagga tgtagggagt gatctggtaa acgcagtagt
      661 tcagccagtt tgaaaaaagc aggtttgcat ggctcctcca tcttaccatc ggctgtttaa
      721 ggggatcatc atggggaaaa taattcttcg gtaccctgat atcaagacct ttggcaatat
      781 ccctgtcata ttccgacttg agggtcagcg gatcatattc tgaatgacct gtgacaaaga
      841 cccttttcag atcccttgaa gcaaccaggt aaatacctgc ttcatccgaa tcggccagta
      901 tttcaagctc actgaccttt tcgatatctt ccctcctgac ttcagtgtac cgtgaatggg
      961 gtgcaagaaa ctcatcatca aaacctctca tcaggatgtt ggttttgtta tagatgcggt
     1021 gattgaacac gccaaatatt ttctctccca ccatgtattt cgggatcccg taatgataat
     1081 acaggccagc ctgtgatgcc cagcaaatga acatggttga ggtaacatga tgatcggccc
     1141 atttcattat cctggtcatc tcttcccagt aatccacctg ttcaaattca aggtgttcga
     1201 taggcgcgcc ggtgatgatc ataccatcgt acttatggcg tgaaacatca tcgaatgttt
     1261 tgtagaatgc atccagatgt tccctgggag tattctttga aagatgcatt tttgtataga
     1321 tgagggtgat ctcaacctgg aggggtgtgt ttgaaagaag cctaaggatc tgtgtttcgg
     1381 tcgtgatctt taccggcatg agattgaata tcaggatctg cagaggcctt atgtcctgat
     1441 gcagcgccct ggtttccttc attacgaaaa tattctcgtc ttcaaggatc ctgactgcag
     1501 gcagattgtc ggagatattt agcggcatag ttcgtacaag ttttaaaaag tcaaaaatca
     1561 ccggaacaaa aaaccgggca tgaaagataa cacataatta ccggttattg ttttcacatc
     1621 tccgggagaa aactcagggc attggttacc ctggatcagg atccggcatc tgataactgc
     1681 catcggaggc ctttgtcagg gtttgtaaag tagcttccct ttcaatgtta cggaactatc
     1741 cccggcggct gcggacattt cccgtgaaga aattccctgg cctgatgatg tcctgtgtat
     1801 cccggctgca gtaactgaag catgttagcg gatcattccg ctgcatgggc tcttaccggc
     1861 aaaatatggc catttcgtta tttttcagct tcccagatgg cagctgcaag cgtaaacaca
     1921 ggaagcaata tctgtccgta taccgggtca taccacccgg agagggcaaa aggacctccc
     1981 agggcaaagc accatacgac aaacgacaac aacgatatca gaagctgctg tatcttgaat
     2041 actttctgaa ttctccggag atacagcggg gtaagcaggc agaacaggcc gaagacaatc
     2101 cattgaagga ttaaggcctc ccgcccttga ggaatattct tcagtattcc ctgtataaag
     2161 atatacccgg ccaccagctc ggccggaatg tacttgaaaa gcctgtcgga atagctgtcc
     2221 ctggccggct gcgcgccttt gccgtcggtt ttcgaagctg cagcctcctg ttttagcctg
     2281 cggatatcac ccgccgatat tatttccctt gccatggctg attggtttat tgattaactg
     2341 taacggattt cttcctcctt ttgcctgatt tgccggcggc accggtttcg agtgccagct
     2401 tgaccgcttt cagggcattg atccttccgt acccgtagaa cctgctgtgt ccttcactgt
     2461 cgtactctcc acctgaaata tcgatcttgt cgcatgtttc ccttattatt ttccttacct
     2521 gagtccattt gagggcagga ttggccgaca aaacaagtgc tgccgttccc gccaccccgg
     2581 ggcatgaggc cgacgttcct ccgaagctgt cggtatagtt gcctccggga ttatatcctg
     2641 ccctgcccag ccggtcggtt gtgaatattc caggagtaag gggatcggga tggttgaagg
     2701 gaggataccc aaaatcgttg cttgggaaac agcaccatat atttttgccg taatcgctgt
     2761 aaatgcttct cctgctcctg tcgttgcacg ccgcaacggc cattaccttt tcatatccgg
     2821 catacccgtc gtagccgcag tcttcattgc cgtttcctgc tgcaaaggta attatgcatc
     2881 cctttccgtt gcgtccgtgg ctgacggcat aatcgattgc gagccgggta ctgtcgggaa
     2941 gatcgaccat ggttgtatga acagggtcat ccgggtcgga ccatgagccg tcttccgggc
     3001 cccagctgca ggagattatg tcggccccgt ggtccgcggc ccatttgaat gccgcggcct
     3061 cggcagcaga ccccaggttg gcactgagcc tgacaggcat aagcatggcg ccgggggcaa
     3121 ctcccgatgc ttccattccc gatgcggcag caaccccggc acatgccgta ccatgattgt
     3181 tataactgct ccggggcctc ggatcattgc tgccggtgac tacatcccgc gagtgaacca
     3241 cttttcctgg tagtctgaat tccggatgat ctatctcaac cccgtcatca atgaccgcta
     3301 tcactatgtt ctttccggtt gaaaagctgt gagcaaggtc agccttcacc ccggcattta
     3361 tcctcctgcc attgatcagg gtacccgcca ggtgccattg cttgggatta atccccttga
     3421 atcctctttt ccggataagc tcgggatgac acagctcaac ttcttccatg gccagcagcg
     3481 actcggcaat actgaagatc ctcaggccgg tgttctcggg tgcacctgca aaataggtat
     3541 tcttcgaata atcgggtttc tgtttgatcc ttagtttgtt gtctttcagg atcttttcac
     3601 agatttctga tctcacctca tcgtggaact taatgaaaat attttcagtg tagaggacca
     3661 gggtcctgcc gtcggcttca accaggacct tgccggcaaa cctgagctcg ggctcctttt
     3721 tgagcatcat tcttgccctg tcccttactg ccacgggatc ctttataggt tctctcagcc
     3781 tgtagaccga aatatccgcc tcggggaact caagcaccag ctcaaagtcc ttcagtgcct
     3841 tccggccccc gggagaacta acggcatttt tgatgctcct tgaacttctt gttcttaaag
     3901 caaccatatc cgtgctttcg tgaagtacgt acttttttcc tgatttgccg cccaaagtat
     3961 aggtgtacat aatttcagag tatgataatt ctaataaaga ggaataaaat taaaacaaga
     4021 tattgcggtt atttagtccc ggttcataca tcctgtattg taaagtcaaa tcctgttgcc
     4081 gacatcaact atccaaattt aaaaaaaaac tccgaagttt ctcatttatg atgttttaat
     4141 tgcattccgg aagatctgaa aaattaaata aaggttgttt tcacaacttc ccggtcatca
     4201 ttttattcac tttcgtcaaa atgcaaaata aaatgcaata ttcaggcaag caaaatgtat
     4261 attgtttaat atgagcatgt tgagttaaat tattcaaaag aaagctgtta aaagggaatt
     4321 tcggtggtaa atctccggtg atgtttgact aatgtatttt atccgtccgt gaactgtcat
     4381 tttcagtttg cagacattgc attttctttg accagtcgct ccactgcatg tctctcttgc
     4441 tcataaaccg gacatttgca acttgacaca caattgccgg gaaattaatt ttacctgagg
     4501 attccgggaa agaaaaagtg gttcagtgaa tacgggaacc agtgcaacgg aaggtaataa
     4561 cagaatacag aaaataaccg gggaacgact acttaaaaaa tgaaaatctt ctaccagata
     4621 acgtcaaaag agttcggcac tgttctcctg aaaaaaagga agattgccaa agcacttaga
     4681 tggtggctgc gccggaacgg tttagcatac acgcatagtt tctttataag ttaggtcagg
     4741 tttcaggcat gaattagtta ggcgaagccg gttttaccgg ctggtgtgtg aagagaagaa
     4801 tcaagaggca ggttcttctc ttcttttttt cagcactatt cggtgatatt ttctcttttc
     4861 catttgataa tttactattt ttaaaggtga ttgaaaacct gtcaaaacca cttaacttaa
     4921 aaccagtgat tatgagaaac cgactgattc ttttcattat tgccattatg gtaccggtaa
     4981 tggtaatgtc gcaggcccgg ttcaacgggc ttgacatgaa tatgggcaat ctttacaggt
     5041 tgtcagatgc aaagacacgt tcaataagtc ctgagaactt cacgggtgaa aaggggaagg
     5101 gaggcatggc gcggcttgag gataaggaca agcccaatgc ggccaactct gctcatgcgg
     5161 cgcgtgatct ggggcagggc tggaaagtta atcccctcgt ccgtatactg cccggggaaa
     5221 cgtttaccat ggctgagatt gaagggcctg gtgcaattca gcatatctgg atgacacccg
     5281 caggcaactg gaggttctcc attatccgga tctattggga tgatgaagca gagccgtcgg
     5341 ttgaatgccc ggtgggtgac tttttcggca tgggctgggg tcagtatgca cctctgtcgt
     5401 cccttgccgt ctgtgtcaat cccgggagcg cgttaaactg ctactgggtg atgccgttca
     5461 ggaaaaagtg caggataaca atggaaaatg tcggcgatca ggatccgatg acccttttct
     5521 accagataga ctatacgctg actgatgttc ctgccgatgc cgcatatttt catgcccagt
     5581 accgcagact taatcccaat gtgacttcag actacacgat catcgacggc ataaagggca
     5641 aaggacacta tgtcggggtt tatatggctg tcggagtgaa caataacgga tggtggggag
     5701 aaggtgagat caagtttttc attgacggcg acaccaggtt ccctacaata tgcggaaccg
     5761 gaactgaaga ttatttctgc ggttcctata attttgaaac caggaagagg aatgacgcgg
     5821 gagttgaagt gggtgattac accgaattct gtacccccta cgcagggctt caccaggtga
     5881 tacgcggcga cggccgttac agggtaatgc agcgctttgg aatgtacagg tggcacatta
     5941 tggatccgat aagattcgaa aaggatctga aggttacaat acaggatctg ggatggagac
     6001 agggtggaag gtatcttgcc cagaaatcag atatcgcgtc agtttgttac tggtaccaga
     6061 ccgagcctca tgcaagattc ccgaaaatac ccggatggag agagctggaa gtttactaat
     6121 caaaatataa tataaatgga acagagtaag aataataatt cggaaaaaaa gaaaggtttg
     6181 gatcgtcgcg ggtttatttc aaggtcatcg ctggcggcaa taggtgcgat tggaacagcg
     6241 ggcctgctgg gttcctgctc cggtaagtca agaaagcccg agctgaaact gccgacgctg
     6301 ctgaacgtgg cgcctgacgg taaattgctt aaggcaggac tgattggctg cgggggcagg
     6361 ggaaccggtg ctgcaatcaa tttcctcgac gccggcccta acctggaaat aaccgctctc
     6421 ggtgatgttt tcagggacaa gctcgatcag tgccgggagc agctgaaaaa agagaggaac
     6481 gtggagattg ctgaggaaaa atgctttgtg ggattcgaca gctttgagaa ggtcattgac
     6541 tcgggtgttg acatagtgct cctgtgcact ccgccgcatt tcaggcccgc ccatgtggag
     6601 gctgcggtgg ctgccgggaa gcatattttc atggaaaaac cctgcgctgt cgatccggtg
     6661 ggtgcgcgca gggttatggt agccgcaaag agggctgccg acaaagagct cagtatagta
     6721 agcggaacaa tccggagggt ccagaaagat ttcatggaaa cccaaaggcg ggttgccagc
     6781 ggacagatcg gcgaaatcgt cagcgcccat attacacgca acggaggatc cctgtgggtg
     6841 atacaaaggc agaagggatg gtccgacatg gaatacatgc tgcgcaactg ggccaatttc
     6901 tgctggctgt caggcgacca tatcgttgaa caattcattc atgaaattga cgtaatgaac
     6961 tggtacctgg gaaaaaaccc cgtgaaggca atggcatggg gcggccgcca gagacgcgtt
     7021 acaggcgacc agtacgattt cttcagcgtg gaatacatct acgacaacgg catgcatacc
     7081 cattgcgccg cccggcagat aacaggatgc agcaacctta ccgaacagtt tattgtcgga
     7141 actgagggtt ttgcaaatgc caggggcatg atctacaatc tcaagggcga ggaagtatgg
     7201 aaatatccct acccggaaga aggctcaacc gatgaaacat ggaaggttaa ggatcctttc
     7261 gtgcaggagc atatcaacct tgtaactgca atcaggacag gtaaacccta taatgacgct
     7321 gaggcacagg tcaattccac cctgataact atcatgggca ggatgtcggc ctatacgggc
     7381 aaggatgtga cctgggatga ggtgcttaac tccgaccttt atctcggacc aaaaacctat
     7441 gaattcgggc cggtaccggg aatccccgaa acccctccgg tgataggggc cgaacccaag
     7501 ctgggctgat atagcggaca taccccgggt tgccttttaa acaccatgga tgtaaatggg
     7561 atgaaacttg aaaacatgtt caggtgtctg cacgggaacc ggaatacaac aacaggctgc
     7621 tccgggtttt tacagagtaa tccccggtca gcgcagccgt gatttttcag atcgcggtgc
     7681 ggctgcagat cattacttta aaattatcag gatatgaaca gacgacaatt tgcaaaaacg
     7741 accatgatgg cctcagtcat tgcttcagcc ggaacccttc cggtactttc gggcctgcgc
     7801 agcactgctg ccccccaact gaagaaaggt attatgtggg gaagcaacgg agtgggaaaa
     7861 accataatcg aaaaattccg gtccgcaaag gcagccggat ttgacggcat tgaaccaatg
     7921 agccatctcg acaggaatga aatactgaag gccagggatg ccacaggtct ttcaattcca
     7981 agcgtatgcg gcgaatacca ctggaaatat ctcctttctg atcccgatcc caaagtgcgg
     8041 cagaacggag tcgaagccct gaaagtgtct cttgaggatg ccaaagcata cggagccgac
     8101 acagtcctcc tcgttcccgg aagagttact gaaacggtgg cgtacgacga gtgctggaac
     8161 aggtcagtgg aagagataaa aaaggtcctg ccgctggctg aaaagctgaa cgtgtgcatt
     8221 gccattgaaa atgtgtggaa cggattcctg ctcagtccgg ttgaagccgc atactacgtt
     8281 gatcagtttg agagcccctg ggtaaaattc tatttcgact gcggtaatat tctcacatat
     8341 ggttatcccg aacaatggat cagaatactg ggtaacagga tagccaaaat ccacattaag
     8401 gaatacagcc gcaaaattgc cgacaagcag ggcagatccg caggtttcgg agtgaagctt
     8461 agggaagggg atgtaaactg gagcgcggtg atggatgccc ttgagagtat aggctattcc
     8521 ggctggacca cagttgaaca gcccggggga gatacacccg aaggattaaa ggacctgtgc
     8581 gacaggctca ggcttatcca gcagagctga tccgccgcgt gatcagtctg ccggggaaat
     8641 tccttgaacc atctttaact tgcctgaaat gaaaaagatc agtctccgcc ctgtttcatg
     8701 ggccattgtt tttgccttat tgcttgtttc ctgctcggga aacaggcagg aacttcagaa
     8761 ggaaggacat cagtttaacg ggtcatattc cggaaaaaac cttgacagga tcgctttccc
     8821 tattgggggc atcggtgccg gaatgttctg tgtt
//
LOCUS       DAOOYG010000108         8798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_8794_length_8798_cov_5.516871, whole genome shotgun sequence.
ACCESSION   DAOOYG010000108 DAOOYG010000000
VERSION     DAOOYG010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8794_length_8798_cov_5.516871"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PK288_08995"
     CDS             complement(<1..260)
                     /locus_tag="PK288_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPM19100.1"
                     /translation="MMKKSDFTILVADDDPDYLFQTISNLGKAGYKTIAAESQSEAEA
                     IIARFKPSLAIFDLMMENDDSGFILCYRLKKKYPDVPVILATA"
     gene            complement(323..2281)
                     /locus_tag="PK288_09000"
     CDS             complement(323..2281)
                     /locus_tag="PK288_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPM19101.1"
                     /translation="MLVYTIKELCRTCYTCVRECPAKAIRIVGGQAEVIDDRCIVCGN
                     CTKVCSQGAKVFLKTTDRVIRLLEKESKVAAIVAPSFPAEFPDYDYKVLIGMIRAIGF
                     ELVLEVSFGADLVADRYRQLVEQDSGYFITSDCPSIVNYVRHYHPDLVDNLAPVVSPM
                     VAMGRVVRSKYGDDTKIVFIGPCVAKKAESSEIDEAITFIELRELFSQYGIGAEMTEP
                     SEFDPPAGGRGAIFPVTRGLLQTANINDDSITGNIIAAEGRTDFQGALKEYEAGLICN
                     QHMELLCCEGCIMGPGLSKNGKQYNRRSLVSSYANRKMNDLDHEAWKHYFDLYSSLDL
                     SVRYKPEENIIVMPDESEIEEVLVSMGKTTIKDQLDCGACGYESCVEHAIAIKRGLAE
                     VEMCLPYTIEKLHNSVKELALSNEKLTSMRQALKQSEKLAHMGQLSAGIAHELNNPLG
                     VVMMYSNILLEECAPDDPVRDDLKLIVEQAGRCKSIVSGLLNFARKNQVNHQRVNIRD
                     LVEHSLESLIVPENVRIEVADMTTSPEAMLDPEQMTQVLTNLVKNAFEAMKEGGSVQI
                     ILEDTLSDVSFTVKDTGTGIKEDDKAKIFEPFFTTKGIGWGTGLGLATAYGIVKMHKG
                     QISVDSNTDPSKGPTGTKFRIVMPRGKE"
     gene            complement(2383..4104)
                     /locus_tag="PK288_09005"
     CDS             complement(2383..4104)
                     /locus_tag="PK288_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPM19102.1"
                     /translation="MLFTIHVNNRKIKAEKGETILSALNRNGIIIPTLCRMKEFTPTG
                     ACRMCVVEVEGKERLVTACSQPVEEWMRIRTHSPRVITARKTIVELLLSNHPDDCLYC
                     DRNLNCELQNLSEELNIRERRIKGKKIKPRLDQSSPAIVRELAKCILCGRCVRVCEEV
                     ITCTSLDFINRGGQTHIGTAMDKDFNFSSCIQCGQCVLVCPTGALHEKYNITEVQEYL
                     NKHDVVKAIQYSPFVPAGIAEELGIRYNKDFDKNLNAILRKIGFDKVYSTGTGTDICI
                     SELADQLSAKLEKNDESPLYITACPAWVKFAEQSMPDIIPCLSTVKSPQQIAGVLIKT
                     MVAGQLKVQPDKIFSVSVSPCMAMKYEARRDGMMRKGISDVDSVLTVRELARLIRLYG
                     IDVYNTAREPADEPLAGRSSSAVLAELSGGLAEGVVRLVSYRLSGKEPDRQLLKKIRG
                     SGSFRDCTVKAGGTEIRVAVVDGLTGLEKLTNALQAGTKYNLVEVMACPGGCIHGGGL
                     PKIASRDDLKNRARLIWQSDETEAISIPEKSPSFLSLCDKLANENMEISDKRIFHTHF
                     ERKDVLL"
     gene            complement(4201..6135)
                     /locus_tag="PK288_09010"
     CDS             complement(4201..6135)
                     /locus_tag="PK288_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPM19103.1"
                     /translation="MDDLKTFLIEKVLLFESDTLSPEAEKVLSQIRREKTDVPVVYVS
                     SNSSSIIAGSEKTYNALKLFAEENEPGTVVIKVGCAGPANYEPVICIQMPGKNKLYFR
                     NITEEKVEPLMNGVFHNDISEEDLIGQSGSKGFELWPGIDFLEDIPFFALQKRLVLGN
                     CGCYDPGRIEEYIAVGGYRTYLKTIRHYTYDEVCDLIERSGLRGRSGGGYLTGLKWKQ
                     ALNTPSNARYLICNAKESDPGAFSDRLVMESDPHRLIEGMAIAAYAIGSSNAILYLKA
                     GSEHARAGIQNAIEQARDYGLLGHNIHSSGYNLDIVVRMEPGAFVCGEETALINSLEG
                     KRGMPQLKPPYPTSAGLAGKPTVINNVETLMNVPLIMQNGPEWYRTIGTFSSPGTKIF
                     ALAGRGRFSGVIEVEMGTSLRTIVEDIADGIRDAREFKAVQIGGASGSFITSDDLDTQ
                     VDFDAMRRQGFSMGSGGFVVIDETSCIVDLVRYYMDFICKESCGKCIPCREGTGRMLE
                     ILENVIRKPANEDTGTTLERFRGVMQLETISSVMKDASLCGLGQNAPNPFVSALKNFR
                     EEFEEHIFDRKCRANVCRGLRTFIIDVEKCTGCTVCAGKCPVNAIYGTRLQPYFIVED
                     KCIGCGICYDVCKFSAVIVK"
     gene            complement(6136..6606)
                     /locus_tag="PK288_09015"
     CDS             complement(6136..6606)
                     /locus_tag="PK288_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPM19104.1"
                     /translation="MTEDIENIITKYRQGRREDLIPLLQEIQERLGHLPEEAIIRVGS
                     HLGLSTTKIYGLATFYDQFRFIPSGRIHISVCHGTSCFMNGSQGIIDKLTEELKTAPG
                     QTSRNGNFSFDIVTCMGGCYHGPVITVNDEYHIHVSQDQIPELIRKLKYIIEND"
     gene            6921..7691
                     /locus_tag="PK288_09020"
     CDS             6921..7691
                     /locus_tag="PK288_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19105.1"
                     /translation="MKRNIRQLLGIIMFIPLTSLLSAQNNNAKDPRKLVAGISAGYNR
                     GIGISANITPFSLLGDLPFSLRFGAGYSFLNPGNAAEARRIFINNNTNGTPEKKGTSL
                     DIRLDFLINKPLFGISTSSLVFGPRFSTFRGNFKYVGGNEDFDVTSHQFGLGAGFENY
                     FKMNPKMNLVLSWGLDIYAPSRLSGHDTSYSPDNDNINPRANNRNNDILFRYGDANRA
                     IKQPFIMPRIMLGVNFGLLHTKPADEFQPAITGSLPVS"
     gene            7902..8156
                     /locus_tag="PK288_09025"
     CDS             7902..8156
                     /locus_tag="PK288_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPM19106.1"
                     /translation="MAQRIEMQICLGSSCFSRGNREVVIMIRDYLKKNHLEDRIVFKG
                     ARCMGLCNHGPNLKINDKIIQGVALSAIEEILEKEFGNLK"
     gene            8175..>8798
                     /locus_tag="PK288_09030"
     CDS             8175..>8798
                     /locus_tag="PK288_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPM19107.1"
                     /translation="MNERKPIFYINEQKCRNTYSCVRVCPVNAIEVRAQKEHPTIIES
                     RCIGCGLCFIDCSPRAVEFRDSRDEVKRLLSSERKTAALVSPSIAPEFVDITDYRKFV
                     GMLRALGFDYVHEDSFGVDLIAAEYADLVSKAEGKYYITANCPPIVKLIEKYHPELVP
                     NLAPLVSPMIATAMVVKELYGEDVATIFIGPCIDNKDEAHLYRGGKLV"
BASE COUNT         2204 a         2288 c         1890 g         2416 t
ORIGIN      
        1 gctgttgcca ggataaccgg cacatcggga tatttcttct ttaacctgta acataggata
       61 aagccgctgt cgtcgttttc catcataaga tcgaatatgg caaggctggg tttgaaacgg
      121 gctataattg cctcagcctc ggactgactt tctgccgcaa tggttttata tcctgccttt
      181 cccaggtttg aaatggtctg aaacaggtaa tcaggatcat catcagccac aagtattgta
      241 aagtcgcttt tcttcatcat ccgaataatt tcgtcataaa accgtctgtt caattatccc
      301 atgccaaaaa cggcaaatcg tctcattcct tccccctggg cattactatc ctgaattttg
      361 taccggtagg gccctttgaa ggatcagtgt tggaatcgac ggagatctgc cccttgtgca
      421 tttttactat cccgtaggcg gtagcaagtc ccaggccggt tccccagcca atacccttgg
      481 ttgtgaagaa aggttcaaag atctttgcct tgtcatcttc ctttatcccc gtgccggtat
      541 cctttactgt aaaagaaaca tcgctcaggg tgtcttccag gataatctgt accgagccgc
      601 cttccttcat ggcttcaaac gcattcttaa caagattggt aagaacctgc gtcatctgct
      661 caggatcaag catggcctcg gggcttgttg tcatgtcagc gacctcaatc ctgacatttt
      721 cgggaacaat caggctctcg aggctgtgct caaccaggtc ccttatatta acacgctgat
      781 ggttaacctg gttctttcgt gcaaaattca gaaggccgct gactatgctt ttacatcttc
      841 ctgcctgctc aacaataagc ttcaggtcgt ccctgacagg gtcgtcgggt gcgcattctt
      901 caaggagaat gttgctgtac atcattacaa cccccagggg attattgagt tcatgggcaa
      961 tccctgctga aagctgaccc atgtgagcca gtttttccga ttgtttcagc gcctgcctca
     1021 tggaagtgag cttctcattc gaaagggcaa gctccttaac ggagttatgc agcttctcaa
     1081 tagtgtacgg aaggcacatt tcaacctcgg caagccctct ttttatcgca atggcgtgtt
     1141 cgacacaact ctcatagccg caggctccgc agtcaagctg gtccttaatg gttgttttcc
     1201 ccatcgaaac gagtacctct tctatttcag actcatcggg cataacgatg atgttttcct
     1261 cgggcttata cctgacgctg agatcgagtg atgagtaaag atcgaaataa tgcttccacg
     1321 cctcatggtc aagatcattc atcttcctgt tggcatagct gcttacaagc gagcgcctgt
     1381 tatactgttt tccgtttttt gacaacccgg ggcccattat gcatccctcg cagcaaagaa
     1441 gttccatatg ctggttgcat atcagccctg cctcatattc cttgagagcc ccctggaaat
     1501 cggttcttcc ctcggcagca ataatattcc cggtaattga atcatcattt atgttggcgg
     1561 tctgaagcaa tcccctggtg accggaaata ttgccccccg cccgccggcc ggaggatcaa
     1621 attccgaggg ctccgtcatc tccgccccta taccgtactg actgaaaagt tccctcagtt
     1681 caatgaatgt gattgcttca tcaatctcgg aactttcggc tttttttgct acacagggac
     1741 cgatgaaaac aatctttgtg tcatccccgt attttgaacg gaccaccctg cccattgcaa
     1801 ccatgggcga taccaccggg gcgaggttat caacaagatc gggatgataa tgcctgacat
     1861 aattaacgat cgaaggacag tcagaggtaa tgaagtatcc tgaatcctgt tccaccagct
     1921 gcctgtacct gtcagcaaca agatcggcac cgaaagatac ctcaagtacc agttcaaatc
     1981 caatggccct gatcatcccg ataagtacct tatagtcata atcaggaaac tcggctggga
     2041 agctcggagc cacaatggct gcaaccttcg attctttttc gaggaggcgg ataaccctgt
     2101 cggtcgtttt caggaaaacc tttgctccct ggctgcatac ctttgtgcag ttaccgcaaa
     2161 cgatgcacct gtcatcaata acttccgcct gccccccgac aatgcgtata gccttggcag
     2221 gacactcccg cacacaggtg tagcaggtcc ggcacagttc ctttatggta tagacaagca
     2281 ttatctggta ttaaaggatt caactgacgg atatccggtg caaaggtaca atggtttgga
     2341 tatcatgaca caatttttca tctcttttgc aactttcggt tactataaaa gaacatcctt
     2401 cctttcaaaa tgagtatgaa atatcctttt atcacttatt tccatgttct catttgcaag
     2461 tttgtcgcac agacttaaaa atgaaggtga tttttccggg attgaaattg cctctgtttc
     2521 atctgattgc cagatcagcc tggccctgtt tttcagatcg tccctggatg ctattttggg
     2581 gagcccgcct ccatggatgc agccgcccgg gcaggccatt acttcaacca ggttgtattt
     2641 tgtaccagcc tggagtgcgt tggtaagctt ttcaagcccg gtcaggccat caaccacggc
     2701 aacccttatc tctgtgcccc cggctttcac cgtgcaatcc ctgaatgatc ccgaacccct
     2761 gatttttttc agcaattgtc tgtcaggctc cttacccgac aaccgatatg aaacgagtct
     2821 gacaaccccc tctgccaatc ctcccgacaa ttccgcaagc acggctgagg aacttctgcc
     2881 ggccagcggt tcatcagccg gttccctggc agtgttgtac acatcaatcc cgtacagcct
     2941 gatcagcctg gcaagctccc gcacagtgag cactgaatca acgtcgctga tcccctttct
     3001 catcattccg tcgcgccttg cctcatactt cattgccatg cagggggata ccgaaaccga
     3061 aaaaatcttg tcaggctgaa ctttcagctg accggcaacc atggtcttaa tcagaacccc
     3121 tgctatctgc tgcggcgact taaccgttga aagacaggga ataatatccg gcatcgattg
     3181 ctccgcgaat tttacccagg ccgggcaggc cgttatatac aacgggctct catcattttt
     3241 ctcaagcttc gccgaaagct gatcagccag ttcagaaata cagatgtccg ttcccgtacc
     3301 tgtcgagtaa accttgtcaa aacctatttt acgcaggatt gcgttcaggt tcttgtcaaa
     3361 atccttgttg tacctgattc ccagctcttc ggcgataccg gcgggaacaa agggagaata
     3421 ctgaatagcc ttcacaacat catgcttgtt aagatattcc tgtacctcgg ttatgttata
     3481 cttttcatgg agcgcacccg tgggacagac aaggacgcat tgaccgcact gaatgcagct
     3541 ggagaaattg aaatccttgt ccatcgcagt acctatatgg gtttgccctc ccctgttgat
     3601 aaaatcaagc gacgtgcagg taatgacctc ctcgcaaacc ctgacgcacc tgccgcataa
     3661 tatgcatttt gccagttccc gtacaatagc cggacttgac tgatcgaggc gtggtttaat
     3721 ctttttcccc ttaatccttc tttccctgat attgagttcc tcagacaaat tctgcagttc
     3781 acagttaaga ttccggtcgc agtaaaggca atcatctgga tggtttgaaa gaagcagttc
     3841 aacgatggtc tttcgggcag tgatcaccct gggcgagtgg gttctgatcc tcatccattc
     3901 ctcaacgggc tgtgaacagg cagtgacgag cctttccttt ccttctacct caacaacaca
     3961 catcctgcat gctcctgtcg gcgtaaactc cttcatcctg cacaaggtcg gaatgatgat
     4021 gccgttgcgg ttaagggccg acaatatggt ttcacccttt tcggccttta tctttctatt
     4081 gttaacgtga atggtgaaca gcatctttca gtttattgca taagtcactt ttaaaataac
     4141 atatctcctt tcattcttcc ggttttaccc ggatccgggc cggtcatcac agccatgctg
     4201 ctattttaca atcaccgcac tgaacttgca gacatcgtag cagattccgc agccgataca
     4261 tttgtcctcc acaatgaagt aaggctggag ccttgttccg tagatggcat taaccgggca
     4321 ttttccggca caaacggtgc agcccgtaca tttctcgaca tctataatga acgtacgcag
     4381 gcctctgcag acatttgccc ggcatttcct gtcaaaaata tgctcctcaa attcctccct
     4441 gaagttctta agtgcgctga cgaaagggtt gggtgcattc tgccccagac cgcagagaga
     4501 tgcatccttc atgacacttg aaatagtttc aagctgcatc acgcccctga atctttcaag
     4561 ggttgttcct gtatcttcgt ttgccggttt tctgataaca ttttcaagga tctcgagcat
     4621 tctcccggta ccttcacggc atggaataca tttgccgcag ctctccttgc agataaaatc
     4681 catgtagtag cgtacaaggt ccacgatgca ggaagtttca tcaatcacca cgaatccgcc
     4741 tgatcccatg ctgaagccct gtcttctcat tgcatcaaag tcaacctggg tgtcaaggtc
     4801 gtcagaggtt atgaatgagc ctgaagcccc tccgatctgg acagccttga attcccttgc
     4861 atcccttatg ccatcggcaa tatcctcgac aattgtcctg aggctggtcc ccatttcaac
     4921 ttctatcact cccgagaatc tgccccggcc ggcaagtgca aagattttcg tgccgggact
     4981 gctgaacgtt ccgattgtgc ggtaccattc cggaccgttc tgcattataa gcggcacatt
     5041 cataagggtt tccacattat tgataacagt gggcttccct gccagtcctg cggatgtggg
     5101 atatggaggt ttcagctggg gcattccccg ttttccttcc aggctgttta tcagtgctgt
     5161 ctcctctccg caaacaaaag ctcccggttc cattctgacc acaatgtcga ggttatagcc
     5221 gctggaatgg atattatggc ccaggagccc gtaatccctg gcctgttcga ttgcattctg
     5281 tatccctgcc ctggcatgct cagaaccggc cttgagatac aggatcgcat tcgaggaacc
     5341 tatcgcatat gcagcaattg ccattccttc aatcagcctg tggggatcac tttccataac
     5401 cagcctgtcg ctgaatgccc cggggtcact ctccttggca ttgcatataa gatatcgtgc
     5461 attactcggt gtattgagag cctgcttcca tttcagccct gtcaggtagc ccccgccgct
     5521 ccggccccgc agcccgctcc tttctataag atcacaaacc tcatcatatg tgtagtgacg
     5581 tatggttttc agataagtcc ggtacccccc cacggcaata tattcctcaa tgcgtccggg
     5641 atcgtagcag ccacagtttc caagaaccag ccttttctga agtgcgaaaa acggaatgtc
     5701 ctcgagaaaa tcaattcccg gccataactc aaatcccttt gaccccgact gacctattaa
     5761 atcttcttcg gaaatatcat tatgaaaaac tccattcatc agaggttcaa ctttctcctc
     5821 agttatattc ctgaagtaaa gtttgttctt gccgggcatc tgaatgcaga taaccggttc
     5881 gtaatttgcc ggacctgcgc agccaacctt tattacgacc gttccgggtt cattctcttc
     5941 ggcaaataat ttcagggcat tgtatgtttt ttcagaaccc gcaatgatgc tgctgctgtt
     6001 cgaagaaaca tagactacag gaacatcagt tttctcgcgc ctgatctgcg ataaaacctt
     6061 ttccgcttcg ggtgaaaggg tatcagattc aaataacagt accttctcta tcaggaatgt
     6121 ctttaaatca tccatctagt cgttttcaat tatgtactta agcttcctga tcagttccgg
     6181 gatctgatcc tggctgacat ggatatgata ttcatcattc accgtaatca cgggtccatg
     6241 gtaacacccg cccatacagg taacaatatc gaagctgaaa ttaccgttgc gcgatgtctg
     6301 gcccggagct gtcttcagtt cctctgttaa tttatcaatg atgccctggg atccattcat
     6361 gaaacacgag gtgccatggc atacgctgat atgaatcctg ccggagggta tgaatctgaa
     6421 ctgatcataa aaagtagcca gcccgtaaat tttcgtcgtg cttaaaccaa gatggcttcc
     6481 cactcttatt atggcttcct ccggaagatg cccgagcctc tcctgtatct cctgcagcag
     6541 cgggataagg tcctcccgcc ttccctgcct gtattttgtt attatattct caatatcttc
     6601 agtcatctct tgcccaaaat accaacacaa atataaaaaa cttattgtta ataaaataac
     6661 aatgtttatt aaataacaat aaactttctc attaaaatca gcccgagagt tgtatgaaag
     6721 aatctgatcg gcacttctgc ccgctccgac actgtcataa agatctccgt cacattttca
     6781 gacagcgtgg gaccgataaa aaacaattcc gtctgaacag attggaaaag taaagaatta
     6841 tgccctgaac cgaccctcca gggatacttt cgcatgaatt tatccgttat aggaatcagt
     6901 attggtcaat taatcattct atgaagagaa atatcagaca attgctcggt ataatcatgt
     6961 ttatacccct gaccagctta ttgtcagcac agaacaataa tgcaaaagat ccccggaagc
     7021 tggttgcagg catcagtgca ggttacaaca gggggatagg catcagtgca aacatcactc
     7081 ctttcagcct gctgggtgat ttgcctttca gcttacgttt cggcgcaggt tattctttcc
     7141 tgaatccggg aaatgcagct gaggctcggc gcatcttcat aaataacaat accaacggaa
     7201 caccggagaa aaagggtacc tcactcgata tcaggctcga ttttctcatt aacaaaccgt
     7261 tattcggcat aagcacttcc agcttggtgt tcggtccgag attttccaca ttcaggggaa
     7321 atttcaaata tgtcggcggc aatgaagatt ttgatgtcac ttctcaccag ttcggtctcg
     7381 gtgccgggtt tgagaactat ttcaaaatga atccaaagat gaacctggtt ttatcctggg
     7441 ggcttgacat ttacgctcca tccaggctgt caggccatga tacctcctac agccctgaca
     7501 acgacaatat aaatccccgc gccaataaca gaaacaatga tatccttttc aggtatggcg
     7561 atgcaaaccg ggctatcaaa caacctttta tcatgccgcg tatcatgctg ggagtgaatt
     7621 tcggactgtt gcacacgaag ccagcggatg aatttcagcc tgccatcacc ggctccctgc
     7681 cggtgtcatg aaaggccagc agacagaatc tcaaacccgg ctgttgcaac gatacaacgg
     7741 attcgttcct ccgctgctga acctgccgga caaaccgcct agacacaaca ggcgatcagc
     7801 ctcatcccct tcagccggcg ctttcacggg acgaatgact tttgtgtctt tctcagtacg
     7861 gaaaggaatt attattaatt ttgtgaattc ttttcgaaat tatggctcag cgaattgaga
     7921 tgcaaatttg ccttggcagt tcctgttttt caaggggcaa cagggaagtg gtaataatga
     7981 tcagggatta tctgaaaaaa aaccatctgg aggatcggat agtcttcaag ggagcccgct
     8041 gcatgggttt atgcaaccat ggtccgaatc ttaaaataaa tgacaaaatt atccaggggg
     8101 ttgctctttc agcaattgag gaaatccttg agaaagagtt tggaaattta aaatagcaaa
     8161 gcccgtaaaa ccaaatgaac gaaagaaaac caatttttta tataaacgaa cagaaatgca
     8221 ggaacaccta ctcctgcgtc agggtttgtc cggtcaatgc cattgaagta agggcccaga
     8281 aagaacatcc gacaattatc gaaagccggt gcataggatg cggcttatgc tttatcgact
     8341 gttccccgag ggcggttgag ttccgcgatt cgagggatga agtgaagcgc ctgctttctt
     8401 cggaaaggaa aaccgcagca cttgtttcgc caagcattgc ccctgaattt gtcgatataa
     8461 ccgattacag gaaatttgtt ggcatgctcc gggccctggg ttttgactat gttcacgaag
     8521 attcctttgg tgttgatctt attgccgccg aatatgccga tcttgtaagc aaggcagaag
     8581 gcaaatatta tattacagcg aattgccctc cgatagttaa gctaattgag aaatatcacc
     8641 ctgagcttgt cccgaacctt gccccccttg tctcaccgat gatagcaacc gcgatggttg
     8701 tcaaggaact gtatggtgaa gatgtggcaa caatattcat tggtccatgt atcgacaaca
     8761 aggatgaagc tcacctttac aggggaggta agcttgtt
//
LOCUS       DAOOYG010000109         8589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9117_length_8589_cov_7.851652, whole genome shotgun sequence.
ACCESSION   DAOOYG010000109 DAOOYG010000000
VERSION     DAOOYG010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9117_length_8589_cov_7.851652"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2614)
                     /locus_tag="PK288_09035"
     CDS             complement(<1..2614)
                     /locus_tag="PK288_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19108.1"
                     /translation="MRNLIPLLMVFMILQSCSDGKKNLVVLSAPAGKMYTSINMGGVT
                     VIPNGRLLTPAGMTFLVAPHPFGLALSSDGETAVTANSGTNPLSISIIRNLSLSPEIM
                     QIPPGHSTDAGILESVFMGLAITPDNSKVYVAGGQANKIFVFDLVTGNKTDSIDCAIS
                     SDGTDYPDGYIGDMAISKDGRHLFAVDQMNFRMLIADTRAMQVTASVPVGRYPFGLAL
                     TNDGKKIYVANVGMFQYSKIGSIEADSDYGKALSFPPFAYNSQEMINGITTDSLVVPG
                     LGDPNSDLAFSVWGIDIEDISKPVVTSRIKTGNLVGQMVEGIPAVGGSSPNSMAVTSS
                     YVFVSNGNNDNITVIDIRNDSVVKEIYLKPDDAVKHFRGVIPFGLDVSPDGRKLFVAE
                     SGINAVGVIDIPSMRVVGHIPSGWFPSKVKVTPDGKRLIVTNAKGYGSGPNGGRNFRE
                     GPEGTYIGSLMKGSISIIDIPSDKELRKMTRQVIANNFRKDKAAKLLKERKGNPVPLY
                     GGQKESPIKHIVFISKENRTYDEVFGQVEKGVGDASIARYGRNVSFSNRDKSMKVENA
                     DVMANHLKLVSEFAMADNFYVDADVSADGHRWLVNTYPNEWVETSVAASYGGNRTYNP
                     ASKAPGSLGMNGSAGAIYPEDYNESGSMWDHLERNNIDFFNFGFSIMFEPAFYHESYK
                     YTGIRQFANFPVPTPIFTRTSKTYPTYNMMIPDQFRVSQFIKEFSEKWLNGRDTMPQL
                     LTVIIPNDHGAGERPEAGYPFRESYMVDNDLAVGRIIEFLSHTPYWKNMAVFITEDDA
                     QNGVDHVDAHRSILMVISPYVKRDYVSKVHYSFGSIFKTFWNILGLPYLNQYDAAATD
                     LSDMFTAVPDFTPYK"
     gene            2816..4264
                     /locus_tag="PK288_09040"
     CDS             2816..4264
                     /locus_tag="PK288_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19109.1"
                     /translation="MKTKPLTGLLLIMVMICTGSGPEMNDKKWPENIQVILENTTALT
                     YKRGDRLPLYLWPAIDPGKLTDTEAEMLVRELDSRGIGLISRWKAGEKESSLETSLVI
                     ARAQTKLGQRVNIEATSLMDSFFNGDIKTAHIDDNGNPFFDDSFGDHKMGCPFALDFR
                     KKEIRARFEYFTEKFRKEGLKVDFIFTDWEIDGPLEVNRAFAASKKCKRCRQHLGEDF
                     SFTEFQKAMREMRSYFQNYACSSVILSRFPDALVGNYAVYPNDGYRYWYDYFEYYVDG
                     QPFIADQNAKYRKWFNDFPLTGYTFAMPVVYTWDQIFGWYDFENTDYRWFYNMLKVAG
                     NAGKSTPQGIPVISFVHWNTVKVASMKPEPVQMSLECYKELLWHMLLRGTDTFFMWCN
                     RSEYAEEIRPVHEVYSAAQQYGRFLEKGIPITFDVPAEPGTVISGLVLGDSVLIRRTD
                     FGSNREPVSVLAGTKTITVGFNPGKCVIIPMN"
     gene            4373..5875
                     /locus_tag="PK288_09045"
     CDS             4373..5875
                     /locus_tag="PK288_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPM19110.1"
                     /translation="MQNPLLPLSSLLLMGAVTGSAPLTNACVKTTDSGKRPNILFLFA
                     DDQRADALGCAGNTYIKTPNIDRLAGNGVRFTNAYVMGGHHGAISAPSRAMLMTGKNL
                     FHVYDVLDGKVTMPAYFSGFGYETFATGKWHNGAASLIATFQKAENVFLGGMSDHFQV
                     PCQDLDSSGKLTDPVIKGFSTDIFAETAVRYLKDYASSGKKNPFFCYVAFTAPHDPRS
                     PQADYTGRYRGEAIPLPGNFMPLHPFEFDDMNVRDETLAPWPRTTEIIQASLADYYGL
                     ITHLDDRVGYIVRTLTELGLADNTIIIYAADNGLAVGSHGLLGKQNLYEHSMKVPLII
                     SGPGIPKNELRNSLIYLYDLYPTLAKLCRLPVPGDIDGKDLVKVITGREKNVRGSLYT
                     AYRNTVRAVRDEEWKLIRYPERNYTQLFNLREDPLEINNLASLPEFMEKTDEMMKLLE
                     KWYAETDDTATLNPKFILPLEYDYKKLRQIRDQYQPGYVIERYFEAADMK"
     gene            complement(5882..6901)
                     /locus_tag="PK288_09050"
     CDS             complement(5882..6901)
                     /locus_tag="PK288_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPM19111.1"
                     /translation="MKNHRPINRIVSVTIRKSLHFLFTAALILILPAGGQSQSREEII
                     VYLKGLGNGSWMFGQMATWVHDENPDIDHPSNWIRKVYDHTGRKPRYACITYDFDDNP
                     FPDSAWNEGVRKVWQEGMIPGVYNFFANPCGGRWNDPVKIDPVFDLNDNPVRSNFYAQ
                     MDRMVSNLRWLGDQGITVVYTPFVELNDRNKWHAKEGSKNAISLYRLVHDYFEGKGLK
                     NIIWAYHTTQGEGALEEYYPGDEYTDVIGKSAYGTGLVFSEYEWAVEKKKNHGKIIWW
                     AELGIRDREDPPRDCFDVLTKLRTAFPELAGFSFWSDAGYYNVTGNLNGPEFMSDPRI
                     VTLLK"
     gene            complement(6976..7650)
                     /locus_tag="PK288_09055"
     CDS             complement(6976..7650)
                     /locus_tag="PK288_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HPM19112.1"
                     /translation="MIHQRLYIPSSAIIFIAVLISTGSCAFSKKRSLDLLKDARDKKY
                     DIVIVPGVPFTNGKWSFVMKGRVCWSKYLYDKGITDNVMYSGAAVYSPYVEAEIMAMY
                     AAGIGIPEEKIYTETLAEHSTENIYYSYRKAMKLGFRRIALATDPFQARMLKRFTRKY
                     VSPEVGIIPFVVDTLQAVQIHITDPEIDYRKAFRENFTALPDRESMRERFRGTKGLNI
                     DWHIYE"
     gene            8032..8538
                     /locus_tag="PK288_09060"
     CDS             8032..8538
                     /locus_tag="PK288_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19113.1"
                     /translation="MVIAHSNRLNPNGREEFEVFPNHSFYWTDNKMQMNLFPPGNRYY
                     GNVVKQPVTAQVALQEIILPEQRGGLQGLTILKNENVPELPAALGAGQQQAGVASGAT
                     GAKLRIRYISGGVPIEEEIYAVVETMTFPTQGMFGVSNNTLWYLDYIFSFKATAGNLE
                     KNTKIFQT"
BASE COUNT         2347 a         2080 c         1855 g         2307 t
ORIGIN      
        1 ctttgtaagg ggtgaaatcg ggaacagccg tgaacatgtc ggacaggtcg gtggcagcag
       61 catcatattg attcaggtaa gggagcccga gaatgttcca gaaggtcttg aagatactac
      121 cgaagctgta atgcaccttg ctcacataat ctcttttgac atagggagaa ataaccatca
      181 ggatgctgcg gtgggcatca acatgatcca ctccgttctg ggcgtcatcc tccgtaatga
      241 aaactgccat atttttccag tagggtgtat gtgaaagaaa ttctattatt ctgccgactg
      301 ccagatcatt atccaccatg taactctccc ggaaaggata tccggcttca ggcctctcac
      361 ctgcaccgtg atcgttcggg attataaccg tcagcagctg cggcattgta tccctgccgt
      421 tcagccactt ttcgctgaac tcctttataa actggcttac cctgaactga tcaggaatca
      481 tcatattata ggtaggataa gtttttgacg ttcttgtgaa aatcggtgtc ggaaccggaa
      541 aatttgcaaa ctgcctgatt ccagtatact tatatgattc atggtaaaaa gccggttcga
      601 acatgatact gaaaccgaaa ttaaagaagt cgatattatt ccgttcaaga tgatcccaca
      661 tggagcccga ctcattgtaa tcttcgggat agattgcgcc cgcggaaccg ttcataccaa
      721 ggcttcccgg tgcctttgac gccggattgt aggtcctgtt gcctccgtag ctggctgcaa
      781 cactggtctc cacccattca ttcggatatg tgttgacgag ccagcgatgg ccgtcggctg
      841 aaacatcggc atcaacataa aaattgtcgg ccatcgcaaa ttctgatacc agtttcagat
      901 ggttcgccat aacgtcggca ttctcaacct tcattgattt atccctgttt gaaaaggaga
      961 cgttcctgcc gtatctggca attgaagcat cgcccacgcc cttttcaacc tggccaaaca
     1021 cttcatcata tgtccggttc tcctttgaaa taaacactat atgcttaata ggtgattcct
     1081 tttgtccgcc gtacaaggga acaggattac ctttccgctc cttcagaagc tttgcagcct
     1141 tatccttccg gaaattattc gcgatcacct gcctggtcat ttttctaagt tctttgtccg
     1201 aaggaatgtc aattatcgaa atgcttccct tcatcaggct cccgatatag gttccctcag
     1261 ggccctccct gaaatttctt ccgccgttgg gtccagagcc gtatcccttg gcatttgtta
     1321 ctatcagtct ttttccgtcg ggtgtaactt taacctttga gggaaaccat ccggaaggta
     1381 tatgtcccac taccctcatc gaaggtatat ctatcacgcc gacagcgttt attccggatt
     1441 cggcgacaaa aagcttcctg ccgtcggggg atacgtcaag cccgaaaggt atgactcccc
     1501 tgaaatgctt aaccgcatca tcaggcttca gatagatctc tttaaccact gaatcgtttc
     1561 ttatatcgat aaccgtgatg ttatcattgt tcccgttgct gacaaataca taggaagatg
     1621 taactgccat tgaattcggg ctcgatccgc caactgccgg aataccttca accatctggc
     1681 ccacaagatt tccggttttt atcctggaag tcacaaccgg ttttgagata tcctcaatat
     1741 caatacccca caccgagaat gccagatcgc tgttgggatc tcccaggccg ggaactacaa
     1801 gcgaatcagt tgtaatgcca tttatcattt cctgcgagtt ataagcaaat ggcgggaagc
     1861 ttaaggcttt tccatagtct gagtctgctt ctatgctgcc tattttactg tactggaaca
     1921 ttcccacgtt tgcgacatag atcttttttc catcatttgt caatgccagt ccaaagggat
     1981 aacgacccac gggaactgac gcggttacct gcatggccct ggtatcggcg atcagcatcc
     2041 tgaaattcat ctggtccacg gcaaaaagat gcctgccgtc tttgctgatg gccatgtccc
     2101 caatgtaacc atccggatag tcggtcccat ctgatgatat ggcgcagtcg attgagtctg
     2161 tcttatttcc ggtgacaagg tcgaaaacaa agattttatt agcctgtccg ccagcaacat
     2221 acactttcga attatcgggg gtaatggcca ggcccatgaa aaccgactca agaattccgg
     2281 catcggtgga atgccctggg ggtatctgca tgatctcggg agacaatgaa agatttctta
     2341 ttattgaaat tgagagcggg tttgtacctg agttggctgt tacagcagtt tcaccgtcac
     2401 tgctcagagc cagcccgaaa ggatgcggtg caaccaagaa agtcattcct gccggtgtga
     2461 gcagacgtcc gttgggaata acagtgacgc ctcccatatt gatggaagta tacattttcc
     2521 ctgcaggagc agaaagtaca acaaggttct ttttcccatc gctgcagctc tgtaaaatca
     2581 taaataccat gagtaacggg atcagatttc tcatttcagg ccagtttggt tagtattaac
     2641 aaatataaag atatcttccc gaattatcct attcagaatg aataatttat atgatcatca
     2701 aatattttct tcggactgca tgccccccgg tcagccataa aaaagcctat atttgaatta
     2761 aacaaaaaat ctatctgatt tcaaggttat actgtcgacc tgaaactaat tcaatatgaa
     2821 gacaaaacca ttaacaggct tgcttctcat catggtaatg atctgtacag ggtccggccc
     2881 tgaaatgaat gataaaaaat ggccggagaa cattcaggtc atacttgaaa atactactgc
     2941 actgacttac aaacgcggcg atcggcttcc cctttatctc tggcctgcca ttgatccggg
     3001 caagcttacc gatacggaag ctgaaatgct tgttagggag cttgacagcc gcgggatcgg
     3061 actcatctca cgctggaagg ccggggaaaa agagtcatca ctcgaaacaa gcctggtaat
     3121 tgcccgggct cagacaaaac tgggccagag ggtgaatatt gaagcgacat ccctcatgga
     3181 ttcattcttc aacggcgaca taaaaaccgc acatattgat gataacggca atcccttctt
     3241 tgatgacagc ttcggagatc ataaaatggg ttgtcccttt gcactggatt tcagaaaaaa
     3301 ggaaatccgt gcccgttttg aatatttcac ggaaaaattc aggaaggagg gattgaaggt
     3361 cgatttcatt tttactgact gggaaatcga cggccccctg gaggtcaatc gtgcttttgc
     3421 tgcatcgaaa aaatgcaaaa ggtgcaggca gcatctgggc gaagattttt ctttcactga
     3481 attccagaag gccatgcgtg agatgaggtc ctactttcag aattatgcct gcagctcggt
     3541 catattatcc agatttcccg atgccctggt agggaattac gcagtttatc caaacgacgg
     3601 gtacaggtac tggtacgact attttgaata ttatgttgac gggcagccct tcattgccga
     3661 ccaaaatgca aaataccgga aatggttcaa tgattttcca ctgacaggtt acactttcgc
     3721 catgcctgtt gtctatacat gggatcagat ctttggctgg tacgattttg agaatactga
     3781 ttacaggtgg ttctacaata tgctgaaggt cgcaggcaat gctggtaaaa gcacgcctca
     3841 aggtattccc gtaataagtt ttgtacattg gaacaccgtt aaagtcgcaa gtatgaaacc
     3901 cgaaccggtt cagatgagcc tggaatgtta caaggaactc ctctggcaca tgcttttaag
     3961 aggaaccgat acctttttca tgtggtgcaa cagaagcgaa tatgccgaag agatcaggcc
     4021 cgttcatgaa gtatattctg cagcacaaca gtacggaaga ttcctcgaaa aagggatacc
     4081 cataactttt gatgtgccgg ctgaacccgg gactgtgatt tccggtctag tccttggcga
     4141 cagcgtgctt atccgccgta cagatttcgg atcaaaccgc gaacctgtaa gcgttctggc
     4201 aggcacaaaa acaattacgg ttggtttcaa ccccgggaaa tgcgtgataa ttccaatgaa
     4261 ctaaacccca ataagccgga ttcctgagcc cgttttcatt aaagttcttt cggattttat
     4321 tgtttacttt acaggctggc cgctttacaa acaaacctga tacaattaca aaatgcagaa
     4381 ccctttatta ccgctttcca gcctgttgct tatgggagct gttaccggaa gcgctcccct
     4441 caccaatgca tgcgtaaaaa ccacagactc cggaaaacgt cccaatatcc tgttcctgtt
     4501 tgccgacgat cagcgcgccg atgccctggg atgtgccgga aacacatata ttaaaacacc
     4561 caatatcgac cgtcttgcag gaaacggagt acggttcacc aatgcctatg tcatgggggg
     4621 tcatcacggc gctatctcgg cgcccagcag ggccatgctg atgacgggga aaaacctgtt
     4681 ccatgtttat gatgtacttg acggaaaggt aactatgcca gcatatttct cggggtttgg
     4741 ttatgaaacg ttcgcaaccg ggaaatggca caatggcgcc gccagcctta tcgccacctt
     4801 tcagaaagcg gaaaatgtat tcctgggcgg aatgagtgat catttccagg tgccatgtca
     4861 ggacctggac agctccggaa agctgactga tcctgtaata aaaggattct ccaccgacat
     4921 tttcgctgaa accgctgtca gatacctgaa ggactatgct tcatcgggga agaagaatcc
     4981 gtttttctgc tatgtcgctt tcactgcccc ccatgacccg agatcgccac aagcggatta
     5041 cacaggccgg tacaggggtg aggcgatccc cctgcccggg aatttcatgc ccctgcatcc
     5101 cttcgaattc gacgatatga acgtacgtga cgagaccctc gctccatggc caagaaccac
     5161 agaaattatc caagcatcac tggccgatta ttacgggctc atcacacacc tcgatgacag
     5221 ggttggctac attgtaagga cactcaccga gctcgggctg gctgataata caattatcat
     5281 ttacgccgcc gataacggtc ttgccgttgg cagccacgga ctgctcggaa aacagaatct
     5341 ttatgaacac agcatgaaag tgccgctaat tatcagcggc cccggcattc ccaaaaatga
     5401 acttaggaat tcactgatat acctctatga cctgtatccg actcttgcta agctctgtcg
     5461 attgcccgtc cccggcgata ttgacggaaa ggatcttgtt aaagtaatca cgggcaggga
     5521 gaaaaacgtg agaggttcgc tgtataccgc atatcgcaac accgtcagag ccgtgaggga
     5581 tgaagagtgg aaactgataa gatatccaga gcgaaattac acgcagcttt tcaatctgag
     5641 ggaggatccg cttgaaatca ataatcttgc ctccctgcct gaattcatgg aaaaaactga
     5701 tgaaatgatg aagcttcttg aaaaatggta tgcggaaaca gatgacactg caacactgaa
     5761 tccgaaattc attctcccct tggaatatga ttacaaaaaa ctcaggcaga tacgggatca
     5821 atatcagccc gggtatgtaa ttgaacgata ttttgaagcc gcggacatga aatgattgcc
     5881 gtcatttcag gagtgttaca atcctcggat ctgacatgaa ttcagggcca ttaaggttcc
     5941 cggtcacatt atagtaaccc gcatcgctcc agaaactgaa gcctgcaagt tcgggaaaag
     6001 cggtccggag ctttgtcaga acatcaaaac aatccctggg gggatcttcg cggtcacgta
     6061 tgccaagctc ggcccaccag ataatttttc catggttctt ctttttctca accgcccact
     6121 cgtattcgga gaaaacaagc ccggtcccat aggctgattt accgataaca tccgtatatt
     6181 catcgccggg ataatattct tccagcgctc cttctccctg ggtggtatgg tatgcccaga
     6241 taatattctt caatcctttt ccttcaaaat agtcatgaac aagcctgtaa agactgattg
     6301 catttttact tccttccttt gcatgccact tgttcctgtc attcagctcg acaaacggag
     6361 tgtaaaccac agttattccc tggtccccga gccacctgag gtttgaaacc atccggtcca
     6421 tctgggcata aaaattcgac ctgaccggat tatcattcaa atcaaacacc ggatcaattt
     6481 taaccggatc gttccaccgg ccaccgcagg gatttgcaaa aaaattataa acgcctggta
     6541 tcatcccttc ctgccagacc tttcttacgc cctcattcca tgctgaatca ggaaagggat
     6601 tatcatcgaa atcatatgtg atgcacgcat aacgcggctt cctgcctgta tgatcataaa
     6661 ctttcctgat ccagtttgaa ggatggtcaa tatcgggatt ctcatcgtga acccaggtag
     6721 ccatctgacc aaacatccac gaaccatttc ccagaccctt cagataaaca attatctcct
     6781 cccgggactg tgactgacca ccggcaggca atatgagaat cagtgctgcc gtaaacaaaa
     6841 aatgaagtga tttcctgatt gtaaccgata caatcctgtt gatcggccga tggtttttca
     6901 tatccggatt tttttgattt cctcaatatg ctgatttatt ccgacagatc caagtcaaat
     6961 ctgaaagata taatgctatt cataaatatg ccagtcaata ttgagtcctt ttgtacccct
     7021 gaaacgttcc ctcatacttt ctctgtcggg cagggctgtg aaattttccc ggaacgcttt
     7081 cctgtaatca atttcaggat cagttatatg tatctgcaca gcctgcaggg tatccacaac
     7141 aaagggtata attcccacct cagggctcac atacttcctt gtaaatcttt taagcatcct
     7201 cgcctgaaaa ggatcagttg ccagggcaat cctccggaat ccaagcttca tggctttccg
     7261 gtatgaataa taaatattct ccgtactgtg ttccgccagg gtttcagtat atattttctc
     7321 ttctggtatc cctatccccg ctgcatacat ggccattatc tcagcttcaa cataaggtga
     7381 ataaaccgca gctccggagt acatgacatt atcggtgatt cccttgtcgt acagatactt
     7441 tgaccagcaa accctaccct tcatcacaaa gctccacttc ccgttcgtga acggcactcc
     7501 cggtactatt acaatatcat atttcttatc ccgtgcatcc ttcaggaggt caaggcttct
     7561 tttctttgaa aatgcacatg agcctgttga aattaataca gcaataaata ttattgcaga
     7621 tgatgggatg tataatcgct gatgtatcat taaaaacagt ttctgaaaag gtatcatttt
     7681 catatttatt caaatctggc aaaatgaggc cgtacagggg aaccggtatt cccgagatcc
     7741 gaacgggagt tttttccttc agatcaatag ttccctgcgc atgaaactgg aatgttgttg
     7801 tgttccctgt gaataacctg atacaatcat caatggaaaa aggattgtac gtctcatcgt
     7861 cgtaatttca ggtaaagtta caattttaaa agatccggat atctaaaaaa agaattcccc
     7921 cttattgtaa ttatttccta attttgtata atagcttaaa cctgaagaat atgagaacac
     7981 atctgtctaa gctcagggta attattgctt ttatatcagt attatgtttc aatggttatt
     8041 gcgcacagca atcgcctgaa tccaaacgga agggaggagt ttgaagtatt tcccaaccat
     8101 agcttttact ggaccgacaa caaaatgcag atgaaccttt tcccccctgg gaaccgctac
     8161 tacggaaatg ttgtgaaaca acctgtcact gcacaggttg cccttcagga aattattctt
     8221 ccggaacaga ggggtggtct tcagggactt actatcttaa agaatgaaaa tgtccctgaa
     8281 cttcccgctg cattaggtgc gggccagcag caggccgggg ttgcatcagg tgccacaggg
     8341 gcaaagctga ggataagata catatcgggc ggagtcccaa tcgaagaaga aatatacgcg
     8401 gtggttgaaa caatgacctt ccccacccag ggtatgtttg gagtttccaa taatacgctc
     8461 tggtaccttg actatatctt ttcattcaaa gccaccgccg ggaacctgga gaagaataca
     8521 aaaatattcc agacctaagt acatgacaaa aatttacagc attgggtaaa tttatctata
     8581 tcgttgcta
//
LOCUS       DAOOYG010000110         8583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9127_length_8583_cov_5.771341, whole genome shotgun sequence.
ACCESSION   DAOOYG010000110 DAOOYG010000000
VERSION     DAOOYG010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8583
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9127_length_8583_cov_5.771341"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="PK288_09065"
     CDS             <1..1127
                     /locus_tag="PK288_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HPM19114.1"
                     /translation="LKTFSIGFEDKDFDESKYQEEAVRYLHTNHSAISCTSKEIADAF
                     PRVVWHSETPLTRTAPTPMLILSKLVRDNNIKVVITGEGSDEILAGYDIFREAIIRRF
                     WASQPSSSLRPTLLKKIYPDIPHLRNTSPNILKMFFGYKLEDTANPLYSHLLRWNNSN
                     HIKKHFSEHIRESVNGYDPLADLAGRLPESFMEWSPLARAQWLETTVFMSGYLLSSQG
                     DRMAMANSVEGRYPFLDYRVIEYCSSLPDKLKLNGLNEKYLLKKLMTGRIPESIVKRP
                     KQPYRAPISSVFMGREKPDYVDEMLSEKMTRLAGIFSHESVAALLAKIEKAGTASEMD
                     NMVLAAVISTHLVHSQFIENNNSEFRNGPLRNLRIIEEKQ"
     gene            1218..2210
                     /gene="nadE"
                     /locus_tag="PK288_09070"
     CDS             1218..2210
                     /gene="nadE"
                     /locus_tag="PK288_09070"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPM19115.1"
                     /translation="MPVRKPFSKDIILLENVGQVVDNIVEQLREDVLLRFKRHGAVVG
                     ISGGIDSSVCLALAAKAFGPDKVLGIMLPEKDSSPDSEMLARELASRFGVRAIKEEIT
                     GALAGFGCYERRDEAVKRVFPEYDPKTWKMKIGIRQSGLFNNIPPIFYLTVIDPSGNA
                     KDKILPANEYLQIVAASNFKQRTRMSMLYYHAEALHYCVVGTPNKHEQEQGFFVKYGD
                     GAGDVMPIGNLYKTQVYQLAEFLGVPKGIIGRTPTTDTYSAEQTQEEFFYQLPFHLMD
                     RFWYGFENGYPAAEVAAVMGETTERVEALFRNFERKRKTTEYLRMPPIRDYYHG"
     gene            2221..3678
                     /locus_tag="PK288_09075"
     CDS             2221..3678
                     /locus_tag="PK288_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPM19116.1"
                     /translation="MNAIDYFFENTYRLDKPFLVGKEEINYKDLYSSSLQLAAWINEM
                     VGTDKHIILLSVNNLFFLKVYLAIIKSGNICIPLDPGIERENYKYIHDLTDPKLVFLT
                     REVEKRLELNSDQAWYPDTLPSSAEKNMDEIPGGEFDREKCAEIIFTSGSTGKPKGVM
                     ISHKNLISNTSSIVEYLQLTLDDRMLVVLPFYYCYGLSLLHTHLRVGGSIVFNNSFIF
                     LGGVLKSLVDYKCTGFAGVPSHFQILLRKSDSFKQTKFPDLKYVTQAGGKLAPIFIDE
                     FREAHPEVRFVVMYGQTEATARLSWLPPEVYDRRKGSMGKGIPGVELKVVNEKGEPIK
                     PGETGEVIARGDNIMMGYFADEEGTKNAIRNGWLYTGDLGTVDEDGYIYLTARSKEII
                     KVRGKRISPKEIEAVILALPEVIDCTIEGVEDEIEGEMLKATVTVRKDSIGSLTKDRV
                     IQHCSQHLSLFKVPQVYEFKDDLTISATGKKIKKV"
     gene            complement(3718..4908)
                     /locus_tag="PK288_09080"
     CDS             complement(3718..4908)
                     /locus_tag="PK288_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPM19117.1"
                     /translation="MTGGNISDEGSSKIDERGVCWNTNTAPTLTSDRTINGTGMGEYS
                     SSLTGLIPATKYYVRAYASNKYGTSFGDEVTFTTSISNGSSVNQIIADHTIVDRYNDI
                     PQFYIDEVKKMLVWGAGMSHSLGYQHGLILLEQYDPKYQVITYLGDPPPAVTSQAMRF
                     GRIFQSGDNTWTSDVNAYCGILKDQASNLNNPYNVFLYAWCWDMTWVNSPGGGLDPEF
                     NVHWAGSAGGGRWGLNNEDQSLTGTPFSMDSYLSAIETYNAHARSNNYKTFTIYTTGP
                     VDDNSGTENGYQRELKHTYIREYVKNHPETIFFDYADILVYNNSGQKHIVNWNDGGTY
                     RPHSQIHPDNLKDYNSLWQMIPADGQGDPLGDDHIGEAGALRIAKAMWWMLARIAGWD
                     GQSK"
     gene            5264..>8583
                     /locus_tag="PK288_09085"
     CDS             5264..>8583
                     /locus_tag="PK288_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4082 domain-containing protein"
                     /protein_id="HPM19118.1"
                     /translation="MSRRILIILAFLLTGHSLYGQLNPGTIGNDQSICYGYSPAGLYF
                     ISAPTGGLLPYSYRWQRSNDLGNTWADITGTTASRLTYSPPVLARPTMFRCRVTDATP
                     TTRYSNAVTINVLTGLSAGSIGTEQTLNYDELPAPLTQIQAPSGGDGNYSFIWQSSAN
                     NLQWSNIEGSNSQGFAPQQMFTTTWFRRIVSDGTCGSVAGNTVQISVDPITLFTTETP
                     SSNSNDSRYNLGTEFSVLADGFITHARIYSHLNEQGLHLVRIYRQMDDTNYELMAGPF
                     EWNFASGFQGWRDFKLPVAVQVEGGSNYMICVTNGLGNNWYVQSNSNFNTIESNPYID
                     YLNSYYGSVDEVPRGNYNNVSYFRDIVFIPFSPGSIGSSQTICYGIVPASLTQTESPD
                     GGSGMFNYQWQSSTDNSTWINIQGANSPDYAPGTLSQSMYFRRLVFSGGLEAASSPVF
                     ITVNPQFVSATLEGTATVLKSTSTTFTLNISGGTPPYTVEYTCNNLLQPQILTTNQIN
                     QISTGQLVTGRYAINIVSVTDSRGCVPETIGDGIIITAADPGFGTRSNNALLIVNSSS
                     SYYQDFTKYVLPYIEWFGIPFEVCNVNSMLIPELSDYGVIVFGHRNVFSDNYPVNNIE
                     DAVFAGSGLYSFDPHLFDFSSDFNVSGNTPSVTSHQIDIITSPEHYITRLHVNDAFNS
                     TNDEIPLMVINNVAITITLNQSDYTLAGGVTLATMSSGPASSPLLQVASHGAGRIVKW
                     SSYEWMFDEPKVLGPVFGMDDLIWRSMVWSARKPFVMQGLPPMITMRVDDVDGTRSYY
                     LDNLRWLEISNEYGFIPWCGTFIDNVGTGFFTKLSELVNAGRATAIPHALTYDNFIYC
                     NQQNLQGFDPVANITYAWNTFAANSIPVSNYLVTHWYLFSADAVPAVSARGVEFIGTH
                     IPTYDLVFPDGYPGNWINCGPYKTDRNGFGGRGDAPYFFAGDITWEIDNQQYNFFNVL
                     TEIQDDGGYEWFPEFNVTEAINRGVRHLRRAINSMALPTLFTHEDRIWMTEPEWRQTM
                     SGITSGLSAYYPEYNLQYTSIDDACTYIKAKEGIEITSVRAEPGVTNIFFSGDNDLGT
                     KCYLFTEAEGQIGFELVDL"
BASE COUNT         2442 a         1935 c         1920 g         2286 t
ORIGIN      
        1 ttcttaaaac tttttccatt gggttcgagg ataaggattt tgacgaaagc aaataccagg
       61 aagaggctgt caggtatctc catacaaacc acagtgcaat tagctgcact tcgaaggaga
      121 tagctgatgc attcccacgg gttgtctggc actcagagac cccactgacc cgtacagccc
      181 ctaccccgat gctgatcctg tcaaaactgg tcagggacaa taacataaaa gtcgtcataa
      241 ccggtgaggg atctgatgag atcctcgcag gttatgatat tttcagggag gccatcatca
      301 ggagattctg ggcttcgcag ccctcatcat cattgcggcc gacgctgctc aagaagatct
      361 accctgacat accgcatctg cgcaatacaa gtcccaacat cctgaagatg ttcttcggat
      421 acaagctgga ggacactgca aatccgctgt actcccacct tctgaggtgg aacaactcca
      481 atcatatcaa gaaacacttt tcggaacata taagggaatc ggttaacgga tatgatcctc
      541 tggcagatct tgccggcagg ttgccggaat cgttcatgga atggtcgcca cttgcccggg
      601 cacaatggct cgagaccact gttttcatgt ccgggtacct gttatcatca cagggtgatc
      661 gcatggcgat ggccaactcg gttgagggac gttatccttt cctcgactac agggtaattg
      721 agtactgctc atctctcccg gacaagctta aactcaacgg actgaatgaa aagtatctgt
      781 taaagaagct gatgaccggt agaatccctg agagtatcgt taaacggccc aagcagccct
      841 atcgcgctcc gatcagcagc gtattcatgg gcagggaaaa accggattat gtggacgaga
      901 tgctgtccga aaagatgacc agacttgccg gtatcttcag tcacgagtct gttgccgcat
      961 tgctggcgaa gatagaaaag gcgggcacag cctcggaaat ggacaacatg gtgcttgccg
     1021 cggtgatatc aactcatctg gttcacagtc agtttattga aaataataat tcagagttcc
     1081 ggaacggacc tctgaggaac ctgagaatta tagaagaaaa acaataacag gttactcctt
     1141 ccctgccggg aaatcacttg acacgagaaa aggatatcgg gtccgggagg ctgtaataat
     1201 ttataaacag aagcattatg ccagtaagaa aaccattctc gaaagacatc atcctgcttg
     1261 aaaatgtcgg acaggtagtt gacaatattg ttgaacagct tcgtgaggac gtactgctga
     1321 ggtttaaaag gcatggggca gttgtgggca taagcggagg gatagactcc tccgtgtgcc
     1381 tggcgctggc cgctaaagcc ttcggtcccg acaaggttct tggcatcatg ctgcccgaga
     1441 aggattccag tcctgacagc gagatgcttg cccgggaact tgcctcccgg ttcggcgtaa
     1501 gagccattaa agaagaaata accggagcac tggcgggatt cggctgctac gagaggcggg
     1561 acgaagcagt gaaacgggtt tttcccgagt atgatccgaa aacatggaag atgaagatcg
     1621 gcatcagaca gagcggattg ttcaacaata tccctcccat tttttatctt accgtgattg
     1681 atccttccgg taacgcaaaa gacaaaatac tgccggccaa tgagtatctg cagatagttg
     1741 cagcttcaaa cttcaagcag cgcaccagga tgtcaatgct ctattatcat gctgaagcac
     1801 ttcattattg tgttgttggc actcccaaca agcatgagca ggaacaggga ttcttcgtca
     1861 aatacggtga tggtgcaggt gatgtaatgc cgataggcaa tctttacaag acacaggtgt
     1921 accagcttgc tgaattcctc ggggtaccga aaggaattat cggacgcact cctaccaccg
     1981 atacctattc ggcagagcag acccaggagg agtttttcta ccagttaccc ttccatctga
     2041 tggacaggtt ctggtacggc tttgaaaacg gttatccggc tgcagaggta gcagctgtta
     2101 tgggtgaaac cacggaacgg gttgaagccc tgttccgcaa cttcgagcgc aagcgtaaaa
     2161 caacggaata cctgcgtatg ccgccgataa gggattatta tcacggataa acctataatt
     2221 atgaatgcaa tagattattt cttcgagaat acttacaggc ttgataaacc gttcctggtc
     2281 ggtaaggagg aaataaatta caaggatctg tattcttcat ctcttcagct tgccgcatgg
     2341 atcaacgaga tggtcggtac agacaaacat attatccttt tatcggtcaa taacctgttc
     2401 tttcttaagg tgtatctggc tatcatcaag tctggaaaca tctgcatccc gctcgatccc
     2461 gggattgaaa gggagaacta taagtatatt catgacctta cggatcccaa actggttttt
     2521 ttaacacgag aagtggaaaa aagactggag ctgaattcag accaggcgtg gtaccctgac
     2581 accctgcctt cttcagcaga gaaaaatatg gatgagatac ccggcggcga atttgacagg
     2641 gagaaatgtg cagaaatcat ctttacctcc ggttccacag gaaagcccaa gggagtaatg
     2701 atatcccaca aaaacctgat ttccaacaca tcatcaattg tagagtacct gcagctgact
     2761 cttgacgaca gaatgctggt agtactgccg ttctactact gttatgggtt gtctctgtta
     2821 cacacacacc taagagttgg aggatcaata gtatttaaca actcctttat tttcctggga
     2881 ggagtgttga aaagtctggt cgattacaaa tgcacaggat ttgcgggggt accaagtcat
     2941 tttcagattc ttttacggaa atcagactca tttaaacaga ccaaattccc cgatctgaag
     3001 tatgtcacgc aggcaggagg caaactggct cctattttca tcgatgaatt ccgggaagcg
     3061 catcctgagg tcaggtttgt ggtaatgtac ggacagactg aagccacagc gaggctttcc
     3121 tggctcccgc cggaggtgta tgacagaaga aagggttcga tgggcaaagg tattcccgga
     3181 gtggaactaa aggtagtaaa tgaaaaggga gaaccaatca agccaggcga gaccggcgaa
     3241 gttattgccc ggggcgataa tataatgatg ggatattttg ctgatgagga gggcacaaaa
     3301 aatgccatcc gcaacggatg gctttacaca ggtgacctcg gcactgttga tgaagatggt
     3361 tatatctacc tcactgcgcg ttcaaaagag atcattaaag tccggggcaa acggatcagt
     3421 cccaaggaaa ttgaagcagt tattttagca ttacccgaag tgattgattg cacaattgag
     3481 ggtgttgaag acgagattga gggtgagatg ctgaaggcaa cagtcacagt cagaaaagac
     3541 agtatcgggt ccttgaccaa agacagggtc attcagcact gctcacagca tctgtctctg
     3601 ttcaaggtac cgcaggttta tgaatttaaa gacgacctga cgatcagtgc aacaggcaag
     3661 aaaataaaga aggtataaga caaccacgac cttaagcaaa cagcgccagg atgaatatca
     3721 tttcgattgg ccatcccagc cggcgattct tgccagcatc caccacatag ccttggctat
     3781 ccgtaaagca cctgcctccc cgatatggtc atcacccaag ggatctccct ggccatcagc
     3841 cgggatcatt tgccacaagg aattgtagtc tttcagatta tcaggatgaa tctgtgaatg
     3901 cgggcgataa gttccgccat cattccaatt gacaatatgc ttctgccctg aattattata
     3961 aactaaaata tcagcatagt cgaaaaatat cgtctccgga tgattcttga catattccct
     4021 gatgtaagtg tgtttcaatt cccgctgata accattttcc gttcctgagt tatcatcaac
     4081 ggggccagtt gtatatattg taaacgtttt gtaattgttg cttctggcat gagcgttgta
     4141 tgtttctatt gccgataaat agctatccat actaaaagga gttccggtca atgactgatc
     4201 ttcattgttc aaaccccagc gacctcctcc agccgagcca gcccaatgaa cattgaactc
     4261 aggatccaag ccccctccgg gtgaatttac ccatgtcatg tcccagcacc atgcataaag
     4321 aaaaacattg tacggattat tcagatttga tgcctggtcc ttcaatatac cacaatacgc
     4381 gtttacatct gaagtccagg tattgtctcc actttgaaaa atccggccaa atctcattgc
     4441 ctgtgatgta acggctggcg gaggatctcc aagatatgta attacctggt atttaggatc
     4501 gtactgttcc aacaggatca aaccatgctg atatcccaat gaatgagaca ttccggctcc
     4561 ccatacgagc atctttttta cctcgtcaat ataaaactgg ggtatatcgt tgtacctgtc
     4621 tacaattgta tgatcagcta tgatctgatt aacagaactg ccatttgaaa tggaagtagt
     4681 gaaagtaact tcatctccaa aagaagtccc gtatttgttc gaggcatatg ctcttacata
     4741 atactttgtt gcaggtatta atcctgtcag actactcgaa tactctccca tgcctgtacc
     4801 gttaatagtt ctatccgagg ttagggttgg tgcagtattc gtgttccagc atactcccct
     4861 ttcatctatt ttggaagagc cttcatccga aatgtttcct ccggtcaaag ctgaatttga
     4921 agtgatttca gtaaccgcca aagtggttaa tgaagggact tcaccggaac agttcagaaa
     4981 caaataagag tttatcaata gacctaaaac caataacctg gctttcatac aaacacatat
     5041 aatagaactc atccacttca attatttcaa aacaaattaa attatatatt gttaagacta
     5101 gacctattat tttcaacaat ataaaaggct catcctgcca cttatcattt tataacgtat
     5161 aatagtcaaa aactaatata cagatatcac caattttata aatttacatc aaaacacctg
     5221 ccttatgaac tcattttttg aatcattaac ataccctctc cggatgtcgc gaagaatatt
     5281 gattattctt gcatttctgc tgacgggcca ttcgctttac ggacaattga atcccgggac
     5341 aatcgggaat gatcagtcga tctgttacgg atactccccg gcaggtttgt atttcatatc
     5401 agccccaaca ggaggtttgt taccctattc atatcgctgg caaagatcca atgacttagg
     5461 gaacacatgg gcagatatca ccggaaccac tgcctcacgc ctgacttact ctcctcctgt
     5521 tcttgccaga ccaacaatgt tccgttgcag ggtaactgat gccactccta caacacgtta
     5581 ttccaacgcg gttaccataa atgtgcttac tggcctgagt gccggaagta tcggaactga
     5641 acaaacattg aactatgatg aattgcctgc acctttgacc cagatccagg caccatcagg
     5701 aggagacggc aactattctt tcatttggca gagctcggcc aataaccttc agtggtcaaa
     5761 tattgagggc agtaattcac agggatttgc gcctcagcaa atgtttacca ccacctggtt
     5821 caggagaatt gtcagtgacg gcacatgtgg ttcggttgcc ggcaacaccg tgcagatcag
     5881 tgttgatccg attactcttt tcactaccga aaccccatct tccaatagta atgatagcag
     5941 gtataacctg ggcactgagt tcagtgtgct ggctgacggt ttcataaccc atgcaaggat
     6001 ttacagtcac ctgaatgagc agggtcttca tttagtaagg atataccggc agatggatga
     6061 cacgaattat gaactcatgg ctggtccctt cgaatggaac tttgcctcag gttttcaggg
     6121 ctggcgcgac ttcaaacttc cagttgccgt tcaggttgaa ggtgggagta actatatgat
     6181 ctgtgttacg aacggcctgg gcaataactg gtatgttcag agtaattcaa actttaacac
     6241 tattgaaagc aatccatata ttgactacct taatagttat tatggttctg ttgatgaggt
     6301 cccaagaggc aattataata acgtctccta tttccgtgac atcgttttca taccgttttc
     6361 tccgggatca attggttctt cacagactat ctgttatggc attgttcctg catctcttac
     6421 tcagactgaa tcacctgatg gtggttcagg aatgtttaat tatcaatggc agtcctcaac
     6481 agataattct acatggataa atattcaggg ggcaaattct ccggactacg cacctggcac
     6541 attatcacaa tctatgtact tcagacgact tgtattcagc ggtgggctgg aagcagcaag
     6601 tagcccggtt tttattacgg tcaatccaca gttcgtttct gccaccctgg agggaacagc
     6661 caccgttctg aaaagcacat caactacatt cacactgaac atttcagggg gtacaccgcc
     6721 ttatacagtc gaatatacat gtaataattt gcttcaaccc cagattttaa caacaaatca
     6781 gattaatcaa atcagcaccg gtcagctggt tactggaagg tatgcaatca acatcgtctc
     6841 agtgacagac tcaagaggtt gtgttcctga aactattgga gatggaatca taatcactgc
     6901 agctgacccc ggatttggga ccaggagcaa taatgcattg cttattgtca attcctcgtc
     6961 atcttattac caggacttta caaaatatgt actgccatat atcgaatggt tcggtattcc
     7021 tttcgaagta tgcaatgtaa acagtatgct gatacctgaa ttatctgact atggagttat
     7081 tgtcttcggt caccgtaatg tcttttcaga taattacccg gttaataata tcgaagacgc
     7141 cgtttttgca ggatcaggac tgtacagttt tgatcctcat ctttttgact tttcaagtga
     7201 cttcaatgtt tcaggaaata ctccttctgt cacatcgcat cagatcgaca taataacaag
     7261 ccctgaacat tacattacca ggctccatgt gaatgacgct ttcaattcaa caaatgatga
     7321 aatacctctg atggttataa ataacgtcgc gataacaata accctaaacc aatcagatta
     7381 taccctggca ggcggagtga ctctggcaac tatgagctcc gggccggcaa gttcacctct
     7441 tcttcaggtt gcatcacatg gtgccgggcg aattgttaaa tggtcttcat atgaatggat
     7501 gtttgatgag cccaaagtgc ttggacctgt ttttggaatg gatgatctga tatggagaag
     7561 catggtatgg tcagcgagga aaccattcgt aatgcagggg cttcctccta tgataaccat
     7621 gagggttgac gatgtggatg gcaccagatc atattatctt gacaacctca ggtggcttga
     7681 aataagtaat gaatatggtt ttataccatg gtgcggaacg ttcatagaca atgttggcac
     7741 tggtttcttc accaagctga gcgaactggt caatgcagga agggctactg caataccgca
     7801 tgcacttaca tatgataatt ttatttattg caatcagcaa aatctccaag gttttgaccc
     7861 tgtcgcaaat atcacctatg cctggaatac atttgcagcg aattccatcc cggtttctaa
     7921 ctatcttgtg acccattggt acttatttag cgctgatgct gttcctgcgg taagtgccag
     7981 gggtgttgaa ttcataggca ctcatatacc aacctatgac cttgttttcc ctgatggtta
     8041 ccctggtaac tggattaact gcggtcccta taaaaccgat cgcaacggat ttggagggag
     8101 aggcgacgca ccttactttt tcgcaggtga tattacctgg gaaattgaca atcaacagta
     8161 taatttcttt aatgtgctta cagaaataca ggatgacgga ggatatgagt ggttccctga
     8221 gttcaatgtg acagaagcaa tcaatcgcgg tgtaaggcat ctcagacggg ctatcaacag
     8281 catggccctg cccacattgt ttacccatga agaccgaatc tggatgacag agcctgaatg
     8341 gagacaaaca atgagtggca ttacctccgg attatcagcg tactatcccg aatacaatct
     8401 tcagtacacc tccatagatg atgcatgcac ctatatcaag gctaaggagg gtattgaaat
     8461 tacaagtgtc agagccgaac ctggtgtaac aaacatcttt ttctcaggag ataatgatct
     8521 gggaacaaaa tgctatctgt tcaccgaggc agaaggacag atagggtttg aactggttga
     8581 cct
//
LOCUS       DAOOYG010000111         8581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9131_length_8581_cov_5.253108, whole genome shotgun sequence.
ACCESSION   DAOOYG010000111 DAOOYG010000000
VERSION     DAOOYG010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8581
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9131_length_8581_cov_5.253108"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PK288_09090"
     CDS             complement(<1..266)
                     /locus_tag="PK288_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPM19119.1"
                     /translation="MNIRRNKHFKPEVSTSSLNDIMFFLLLFFLIVSTLANPNVIKVL
                     LPTSRSAQNINKQQINLTVTADKRYYINQKEILFQQLEQELREQT"
     gene            complement(268..924)
                     /locus_tag="PK288_09095"
     CDS             complement(268..924)
                     /locus_tag="PK288_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPM19120.1"
                     /translation="MILSLYDSLLQNTVTSGQVTTILDLLMKGGIIMIPIILLSLISV
                     YIFIERYVYIKRSSFIDKGLVPAILTELREGNMDNAVIRARNDQASVGRILESGLDKF
                     GKPVSDVERNLETTINLEITKMENYTGYLGIIAGIAPMLGFIGTISGVIKIFYNISLA
                     DNISIGIIAGGLYQKMICSGAGLIVGVLAYAAYHYLQMKIDRYTIRLQESLVQFLKAF
                     "
     gene            complement(1070..1831)
                     /gene="truA"
                     /locus_tag="PK288_09100"
     CDS             complement(1070..1831)
                     /gene="truA"
                     /locus_tag="PK288_09100"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPM19121.1"
                     /translation="MKTRYFIYVAFNGSRYHGWQVQPNSTSVQKILNDALSHVLREKI
                     CTTGAGRTDAGVNAFAFCAHFDCSVPDLSLRENTIFRLNRILPADIAVLSIKRTASDA
                     HARYSAISRTYRYYISRKKDPFSEQFRWYVYGNLDICEMNKAAKILLSHTDFTSFSRL
                     HSGNKTNICKIMSASWKIENDQLIFTIVADRFLRNMVRAIVGTMVNVGRGKITAGQFE
                     EIIIAKDRARAGMTAPARGLFLAGIEYPEELFQVE"
     gene            2079..3809
                     /locus_tag="PK288_09105"
     CDS             2079..3809
                     /locus_tag="PK288_09105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPM19122.1"
                     /translation="MLEKLDLSWIDYLIMLIYFVFVLGIGWFLRKYMKNANAFLEAGR
                     SIPAWVAGLAFISTNLGALEVMGMTGSGMKYGMLTTHFYWIGAIPAMIFLAVFMMPFY
                     YGSKARSVPEFLKLRYDEKTRGLNAILFAVMTILASGISMYALAILMEKVLGWDFNIS
                     LWVSALIVLGYTYLGGLSSAIYNEVLQFFIIVIGLLPLVFLSLKDVGGWAGLKESLAP
                     ISTDMGYAADAWTTTWKYSGHATSNPLGVEWFGILFGLGFVLSFGYWCTNFLIVQRAF
                     AAESVTASRKVPLIGAIPKMFIPFLIIVPGIISLALWKDPSKNFVMPLDAGGKPIYDY
                     TIIMLLRQYLPAGVLGLGITALLASFMSGMAGNVSAFNTVWTYDIYQSYIRRPTGNEE
                     ADGKHYLNVGRVATIFGVLLSIAAAYIATKFGNIMDFLQTIFSMINAPLFAVIFLGMF
                     WRRTTGHAAFIGLLSGFLLALLHHGLTVPEGATTLVKGGWLGVVHTYPVEMAQNFWTA
                     IIAFSSATLITVFLSLVTRQIKTEDDLRGLVYSLTPRLKEENVPWYQRPAGLAVIVAV
                     ICIILSIMFW"
     gene            3822..4037
                     /locus_tag="PK288_09110"
     CDS             3822..4037
                     /locus_tag="PK288_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19123.1"
                     /translation="MVDIRIPIGLMFTILGVLITVFGLSTHSNSEMYLRSLGVNVNII
                     MGIFMLVFGLIMLYLARKKKDPGTADG"
     gene            4227..5267
                     /locus_tag="PK288_09115"
     CDS             4227..5267
                     /locus_tag="PK288_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HPM19124.1"
                     /translation="MRGFASDNNSGVHPEILAELSRINEGHVIGYGSDEYTDKARALF
                     REIFGDETETYFVFNGTAANVLGLSSVARSWNSIVTASSAHIEEDECGAPEKFIGCKV
                     LTVDSPDGKLTPGMVEKHMRGFDFEHHSQPGVVSISQTTEMGTVYTPSEIRKLADYVH
                     ANGMFLHMDGARIANAAVSLGLPFRAFTTDSGVDVLSFGGTKNGMMYGEAVCFLRKGL
                     SGEFRYIRKQGMQLASKMRFISAQYIAYFRNDLWKTCASHSNLMAGLLARKISEFSEL
                     RITQPTEANGVFVVMPRDLAEKLSRSYFFYPWNESIGEYRLMTSWDTTGEDIEMFAAL
                     LKRELKKAQRFR"
     gene            complement(5385..6167)
                     /locus_tag="PK288_09120"
     CDS             complement(5385..6167)
                     /locus_tag="PK288_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPM19125.1"
                     /translation="MAKEKIIAPPFDDIVFENRNKEYGAYRLRKKYKRNVIISMLIAV
                     IIFSTAIIIPYLNAKAAESRARARAEREVEIKMENLDQPTEQVAPPPPPPPPPQEAVQ
                     QSRYVPPVVVDSVKPEEAAQLMTADQAQVEVRDEEVIEFVQEVKEEVKEEEPEAEPFV
                     VVEEMPMFPGGDAALLQFIAEHTQYPEVAKENNIQGKVIVRFCVTSKGTVDRVSVLKG
                     VDPELDKEAMRVVTTLPPFKPGKQGGIPVPVWYMVPINFTLK"
     gene            complement(6187..6855)
                     /locus_tag="PK288_09125"
     CDS             complement(6187..6855)
                     /locus_tag="PK288_09125"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPM19126.1"
                     /translation="MAEIIQAEKGGGKKRAKRILPHMDMTPMVDLMMLLITFFMLTTS
                     MSKAKVMDITLPDKVIDPEKQQAPKISASRTLNIILGPENRLFWYPGGVKPEEYNNLP
                     PLMETNFSADGIRKLLLDRNRALFKKINDFNQQVIRGEIRITQDSVQGAIRQLKNVDD
                     TGPIVLIKAYKEANYGNFVDILDEMNICGIARYTFVDIAWYEEKMVEAAVARGSSPAA
                     TSSN"
     gene            complement(6857..7495)
                     /locus_tag="PK288_09130"
     CDS             complement(6857..7495)
                     /locus_tag="PK288_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPM19127.1"
                     /translation="MPKIKIPHKSPRIDMTPMVDTFAVILIFLMLTATMSSPEPATVD
                     TPFSISEKPTPDFNAMTFLISADGKVFMNFDNGPDTLLKYRPKVLAEMGKRYGIEFTE
                     KQLREFQMYPSSMGVRIENMQAFLDAKDAAERKVYQEGIPMDSTNNQLAMWIQYARTV
                     NPNIQACIRGDSKVEFPKVKRVLDILQDLNVKRFSLVTSLEAAKINLEEIQQ"
     gene            complement(7533..8351)
                     /locus_tag="PK288_09135"
     CDS             complement(7533..8351)
                     /locus_tag="PK288_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPM19128.1"
                     /translation="MSKQGSSFKDALQNIFAVSAILIAFIVSYLLFVNIMGNPVNFEG
                     GNPKGHALEGNYLGIIYKGGFIVPILMTCVLTLVIFVFERLITLSRAKGKGRLNSFVK
                     KLQDLLENDKIEEAVEACDKQKGSLANVMKAGLIRYRDLQADKELERDQKILSIQKSF
                     EEATALELPMLSRNMVIFSTLASISVLIGLIGTVLGMIRAFAALAQSGAPDALALSQG
                     ISEALVNTAFGITGSTLSILAFNYFSSYIDAYTFKIDEAGFSLTQNFAASLKSR"
BASE COUNT         2186 a         1816 c         1970 g         2609 t
ORIGIN      
        1 gtctgttccc tcagttcctg ctccagctgc tgaaacagga tttccttctg atttatgtaa
       61 tatcttttgt ccgctgttac agtcaggttt atctgttgtt tattgatgtt ctgggccgac
      121 ctggaggtag gcagaagcac ctttatgaca ttcggattag caagggtgga cactatcaga
      181 aagaaaagaa gcagaaagaa cattatatcg ttcagcgagg atgttgatac ttcgggtttg
      241 aaatgcttgt ttctcctgat attcatttta aaatgccttt aagaactgta caagtgattc
      301 ctggagcctt attgtgtatc tgtcgatctt catttgaaga taatggtagg cggcatatgc
      361 aagaacgccc acaatcagtc ctgccccgct gcagatcatt ttctggtaga gccctcctgc
      421 aatgataccg atactgatgt tgtcggcaag ggaaatatta taaaagattt ttattacccc
      481 ggaaatggtg ccaatgaaac ccagcatggg ggcaattccg gcaattatgc caagataacc
      541 ggtatagttt tccatttttg tgatttccag gttaatggtt gtttcaagat ttctttcgac
      601 atcacttacc ggtttgccga atttatcaag tccgctttca agtattctgc ctaccgaggc
      661 ctgatcattc ctggcacgga tcacagcatt gtccatgttc ccctccctga gctcagtgag
      721 aatggcaggt acaagtccct tgtcaataaa agatgatctt tttatatata cgtacctttc
      781 gataaagatg taaacactga tcagtgaaag cagtataatg ggtatcatga ttatgccgcc
      841 tttcatcagg aggtcgagaa tggttgtaac ctgtcctgat gtcacggtgt tctgcaataa
      901 tgaatcataa agacttaata tcatagggaa ttcaattttt aatgttggtt tttcatatca
      961 aacgacgaaa gtattcaatt atttccaatg ttgtaaaacg gttaatagat tgttgaatgt
     1021 ctgtattctg ttgatagaaa tttaattatt taccgggtta acaattagtt cattccacct
     1081 gaaacaactc ttccggatat tctatgcctg ccaggaacaa tccccgtgca ggagctgtca
     1141 ttcctgccct ggccctgtct tttgcaatga ttatttcttc aaactgtccg gcagttatct
     1201 tacctctgcc gacatttacc atggtaccca caatcgcgcg taccatattc cttaagaacc
     1261 tgtcagccac tatggtgaat atcagttgat cattttcaat tttccatgat gcggacatga
     1321 ttttgcagat gttggtttta ttgcccgaat gaagccggct gaaacttgtg aagtcggtat
     1381 gtgaaagcag gatttttgca gccttgttca tttcgcatat atcaagattg ccataaacat
     1441 accatcggaa ttgttcgctg aaaggatcct tttttcttga aatgtaatac ctgtatgttc
     1501 tggatatggc gctgtagcgt gcatgggcat cagaagcagt tctttttatt gacagcacgg
     1561 caatgtcagc cggaaggatc ctgttgagcc tgaatatggt gttttcgcgc aggctcaggt
     1621 cgggaacgga acagtcaaaa tgcgcacaga aggcaaaggc attgactcct gcatcggttc
     1681 tgcctgcccc ggtagtgcat attttttccc tgagcacatg actcagggca tcattgagta
     1741 tcttctggac ggaggttgaa tttggctgca cctgccagcc atggtagcgt gagccgttga
     1801 aagcgacgta tataaagtac ctggtcttca aaataaaatt tatgcaaata tatttaacta
     1861 aatgcatttc aaaaaacagc ggatccggca acggggctta tagctggtgt tgattgtaac
     1921 cttgaagaaa tcaacagatt tctttattga tgccggaaaa attaatatcc ttcattgaaa
     1981 tcccgatctt aataagtatt tttactcgga atgataaagt catttaacat cctgatcgaa
     2041 aaaaattttt gtttgaaaaa aacttaattc cgtttgtcat gcttgagaaa ctagatttga
     2101 gctggataga ttatctgata atgttaatct attttgtttt tgttcttgga ataggatggt
     2161 tcctgcggaa atacatgaaa aacgcaaatg cgtttcttga agcagggcga tcaattcctg
     2221 catgggtggc cgggctggcc ttcatatcaa ccaatcttgg agccctggag gttatgggta
     2281 tgacgggttc agggatgaaa tacggcatgc ttaccactca tttttactgg atcggtgcaa
     2341 ttcccgcaat gatcttcctg gctgtgttca tgatgccgtt ctattacgga tcaaaggccc
     2401 gctctgtacc ggagtttctg aagctgcggt atgatgaaaa aaccagggga ctgaatgcca
     2461 tcctgtttgc tgtgatgacc atcctggctt ccggaatttc aatgtatgca ctggcaatcc
     2521 ttatggaaaa ggtactggga tgggatttca atatcagcct ttgggtgtcg gcgcttattg
     2581 tgctgggcta tacatatctc ggaggattgt catctgcaat ctacaacgag gtacttcagt
     2641 tcttcattat tgtgatcgga ttgcttccgc ttgttttcct gagccttaag gatgttggcg
     2701 gatgggcggg actcaaggag agcctggcac ccatttcaac cgatatggga tatgctgccg
     2761 atgcctggac gacaacctgg aaatactcgg gtcatgcaac atccaacccc ctgggcgtcg
     2821 aatggttcgg tattttattc ggcctgggat ttgtcctgtc attcggttac tggtgtacca
     2881 acttccttat cgtgcagagg gccttcgcgg cagaatcggt tacagcatcg aggaaggttc
     2941 ctttgatcgg tgcaattcct aaaatgttca ttccgttcct gataattgta ccggggatca
     3001 tttcattggc cctctggaag gatccttcaa agaattttgt catgcctctt gatgccggcg
     3061 gaaaacccat atatgattac acaataatca tgcttctccg gcaatacctg cctgcaggtg
     3121 tccttggcct tggtattact gcactgcttg catccttcat gtcgggaatg gcgggaaatg
     3181 tatctgcatt taataccgtt tggacctatg acatatacca gagctatata agaaggccaa
     3241 ctggaaatga ggaggctgac ggcaagcatt atctgaatgt gggcagggtt gctaccattt
     3301 ttggagtgct gttgagtatt gccgcagcct atattgcaac caagttcgga aacattatgg
     3361 actttcttca gacaatattc tcaatgatca atgccccgct gtttgcagtt atttttcttg
     3421 gaatgttctg gagacgaact acgggccatg ctgcatttat cggactcctg tcgggatttc
     3481 tgctggcctt gctgcatcac gggctgactg ttcccgaagg tgctactacg cttgttaaag
     3541 gaggctggct gggagttgtt cacacttacc cggttgaaat ggctcaaaat ttctggacgg
     3601 caatcatcgc tttcagttct gctaccctga tcacagtgtt tttgtcgctc gtgacacgac
     3661 aaatcaaaac tgaagacgat ttaaggggcc tggtttattc tcttactcca aggctgaagg
     3721 aagagaatgt tccatggtat cagcgccctg cagggctggc tgtcattgta gccgtgattt
     3781 gcataatttt aagtataatg ttctggtagg aggaaataaa aatggttgat ataagaatac
     3841 caataggact gatgttcaca attctgggag tcttgataac tgttttcggg ttgagcacac
     3901 attcaaacag tgaaatgtat ctcagatcgc ttggagtgaa tgtcaatatt ataatgggca
     3961 tcttcatgct tgtttttggc ctgataatgc tgtatctggc caggaaaaag aaggatcccg
     4021 gtacagctga cggttaaatc tgtcagaaag aatcgggcgc agggctgcat gaaccctttt
     4081 ctcccgatcc ggaattgtaa tgctctgaat gcctgaaagc agttgtcttt gccgggcgga
     4141 aaccggtata tcctgcttgc cttatataat tggttaacat tctgatgcgg gtaaattaat
     4201 actggaacaa tcgaggatta aaatatatga ggggattcgc aagtgataat aattcaggag
     4261 ttcatcctga gattctggca gagctttcca ggataaatga gggacacgta atcgggtacg
     4321 gttcggatga atatactgat aaagccaggg ccttgttcag ggagatcttt ggcgatgaaa
     4381 ccgagaccta ctttgttttt aacggcactg cagccaatgt attgggcctc agcagtgttg
     4441 cgaggtcgtg gaattcaatt gtcacagctt catcggctca tattgaagaa gatgaatgcg
     4501 gtgccccgga gaaatttatt ggctgcaagg ttctgacagt cgattctccc gacgggaaat
     4561 taactccagg aatggttgag aagcatatgc gcggatttga ttttgagcat cattcgcagc
     4621 cgggggttgt ttcaatttcc cagaccactg aaatgggcac tgtatatact ccgtcagaaa
     4681 tcaggaagct tgcagactat gtccatgcca acgggatgtt tcttcacatg gacggtgcaa
     4741 ggatcgccaa tgcggccgtt tcactgggct tgcctttcag ggcatttacg actgattcgg
     4801 gagttgatgt gctttctttc ggaggaacga aaaacggaat gatgtatggg gaagcggtat
     4861 gcttcctgcg gaagggtctt tcgggagagt tcaggtatat caggaaacag gggatgcagc
     4921 ttgcatccaa aatgagattc atttctgccc agtatattgc atatttcaga aatgatctct
     4981 ggaaaacgtg tgcttcgcat tccaatttga tggcagggct tcttgcccgg aaaataagtg
     5041 aattcagtga attaaggatt acacagccaa ccgaagctaa cggcgtgttt gttgtcatgc
     5101 cgcgtgacct ggctgaaaaa ctgagcagat catatttctt ttatccatgg aacgaaagca
     5161 tcggcgaata ccggcttatg accagctggg atacgacagg ggaagatatt gaaatgtttg
     5221 cggccctcct gaagagggaa cttaaaaagg ctcagaggtt ccggtgatat ggaacaggca
     5281 tggcccggat agcggttatt tggtggtttg aaaggcaggc cttataatac aaaaagccgt
     5341 ttcattttat tttgaaacgg cttttcttta taatcggaat ttatttattt aagagtaaag
     5401 tttattggta ccatgtacca cacaggcaca ggaattccgc cctgtttgcc tggtttaaac
     5461 ggtggaagtg ttgttacaac tctcatagct tctttatcaa gctcagggtc tacgccctta
     5521 agaacagaaa ccctgtcaac ggtacctttt gaggtaacac agaacctgac aatgactttg
     5581 ccctgaatgt tattttcctt tgctacctca ggatactgag tgtgttcggc aataaactgg
     5641 agaagtgcgg catcacctcc cgggaacatg ggcatttcct ccacaacaac gaaaggttct
     5701 gcttcgggtt cctcttcctt gacttcttcc tttacctcct gaacaaattc aataacctct
     5761 tcatccctta cttcaacctg tgcctggtcg gcagtcatca gctgagctgc ctcttcaggt
     5821 ttgacagagt caacaacaac agggggtacg tatctcgact gctgaactgc ctcctggggc
     5881 ggcggcggcg ggggaggcgg cggggcaacc tgttctgtgg gctgatccag gttctccatt
     5941 ttaatttcca cttctctttc agcccgggct cttgccctgc tttccgctgc cttggcattc
     6001 aggtagggaa tgattattgc agtgctgaaa atgatcactg caatcagcat tgagataatt
     6061 acattgcgtt tgtatttctt tctcagcctg taagcaccgt attccttatt cctattttcg
     6121 aagacgatat catcgaaagg aggcgcaata attttttctt ttgccatatc agtcaaggtt
     6181 tacgggttaa ttggatgaag ttgcggctgg cgaagagcct cttgcaacag cggcttcaac
     6241 catcttttct tcataccatg caatgtcaac aaacgtatac cgggcaattc cacagatatt
     6301 catctcgtcg agaatatcga caaaattccc gtagttagct tccttataag cttttataag
     6361 tacaatcggg cctgtgtcgt ccacgttctt caattgtctt attgcacctt gtacggagtc
     6421 ctgcgttatt ctgatttcac ctcttatcac ctgttggttg aaatcattga tttttttaaa
     6481 cagggccctg ttcctgtcaa ggagcagctt acgaattcca tcggcgctga aattggtttc
     6541 catcaatgga ggaagattgt tatattcttc cggctttacc ccgcccgggt accagaataa
     6601 tctgttttcc ggtccgagaa ttatgttcag tgtacgactg gccgagattt tgggagcctg
     6661 ttgcttttca ggatctatta ccttgtcggg cagggtaata tccataacct tcgctttgct
     6721 cattgaagtt gtgagcataa aaaaggtaat tagcaacatc atcaggtcga ccatgggggt
     6781 catgtccatg tgcgggagaa ttctctttgc ccttttcttc ccgccgcctt tttccgcttg
     6841 aattatttct gccatatcac tgttgtattt cttccaggtt aatttttgca gcttcgagac
     6901 tggtaaccag actaaacctc ttcacattca ggtcctggag tatgtcgagg accctcttga
     6961 ccttcggaaa ttcaactttt gagtctcccc tgatacaggc ctggatgttg ggatttaccg
     7021 tacgggcgta ctggatccac atggcaagct gattgttggt ggaatccatt ggaatccctt
     7081 cctgatatac cttccgctcg gcagcatcct tggcatcaag gaatgcctgc atattttcta
     7141 tgcggacgcc catggaggaa ggatacatct gaaactccct gagctgcttt tccgtgaact
     7201 cgattccgta tctcttaccc atttcagcca gaacttttgg cctgtatttc aagagggtat
     7261 caggtccgtt atcgaaattc atgaagactt ttccgtcagc tgatatcagg aatgtcattg
     7321 cattgaaatc aggagttggt ttctctgata ttgaaaaagg cgtatcaaca gtggccggtt
     7381 caggtgaact catggtagct gtcagcataa ggaaaataag tatgacagcg aatgtgtcca
     7441 ccatcggggt catgtcgatc ctgggcgact tatgtggtat tttgatcttt ggcatttttt
     7501 cagttatttg atattaaacc aaaagataca ttttacctgc ttttcagtga agcagcgaaa
     7561 ttctgcgtca gactgaaacc tgcttcatca attttgaagg tatatgcgtc aatatacgat
     7621 gaaaaatagt taaatgcaag aattgacagg gttgaacctg tgataccgaa tgcagtgttc
     7681 acaagcgctt cggaaatacc ctgtgaaagg gcaagagcat ctggtgcacc tgactgggca
     7741 agggcagcga aggccctgat cattccgaga accgttccga tcagcccgat caacactgaa
     7801 atggaggcaa gggtggagaa tatcaccatg ttccttgaaa gcatcgggag ctcaagagct
     7861 gtagcttctt cgaatgattt ctggatggag aggatcttct gatccctttc cagttccttg
     7921 tctgcctgca gatccctgta tctgatcaga cctgctttca tgacatttgc cagcgaccct
     7981 ttctgtttgt cgcatgcttc aacagcttct tcgatcttgt cattctcgag cagatcctga
     8041 agtttcttca cgaaagaatt cagtctgcct tttccttttg ctcttgaaag tgtaatcagt
     8101 ctttcgaaaa cgaaaatgac cagtgtcaga acacatgtca taagtatggg tacaatgaag
     8161 ccacccttgt aaatgattcc aagatagttt ccttccaggg catgtccttt cggattgccg
     8221 ccttcgaagt taaccgggtt gcccattata ttcacgaaca gcaaatagct tacgataaaa
     8281 gctattaaaa tggcacttac tgcaaaaatg ttctgcagag catctttgaa cgaacttcct
     8341 tgtttactca tttttatcga ttttggttag gttaaaattt ttaatgattt gcaaatatat
     8401 attttcaaat gtttttttcc aagcacgcca ttaaaattga cttaatttaa aatattttaa
     8461 aatagtttaa gacttactga tttttcagcc tatcctattt cgctgtcccg atatacaaag
     8521 aaaaaggttc cggatttctg acgaaaatac cggaaccttt atttaatatt attgctcgag
     8581 t
//
LOCUS       DAOOYG010000112         8522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9209_length_8522_cov_7.922405, whole genome shotgun sequence.
ACCESSION   DAOOYG010000112 DAOOYG010000000
VERSION     DAOOYG010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9209_length_8522_cov_7.922405"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PK288_09140"
     CDS             complement(<1..269)
                     /locus_tag="PK288_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HPM19129.1"
                     /translation="MIKYHKSSSGKIIAELTDNKVKIAEVGDILDLIAEIGQHDCNAM
                     IVNESSLNKDFFDLKTRFAGEILQKFSNYRVQLAITGNFSKYPGK"
     gene            complement(458..1588)
                     /locus_tag="PK288_09145"
     CDS             complement(458..1588)
                     /locus_tag="PK288_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19130.1"
                     /translation="MKKLLTFVTLCLFISCSVTEETKIPCYAWLSGPGEATDADLAAK
                     FKFLKDKGIDGLMYSGGQDPLTYKRVGKLAKEAGMEFHTWIPTLVQQPRPELRPELYA
                     VNGLGESAYDKPAYVDHYKFLCPNREEVYQFLENMYGKIADLPEVDGIHLDFIRFPDV
                     ILARGLWEKYGLVMDREFPQYDYCYCDKCVADFKTKTGIDIKSVPDPSSVQEWKQFRY
                     DLITDMVNRLAVMVHAKNKQINAAVFPGPNSIAKKIVRQEWDKWDIDAFFPMNYNDFY
                     LEGPEWIGAVSKEAVTTINNSKPVYSGLFICPEPEKKAAEKDPENHGLVPGELEAAIR
                     ESMENGAAGICLFTPDRMTAEGWKVFEKAIRTKYVNKIFPSE"
     gene            complement(1697..2572)
                     /locus_tag="PK288_09150"
     CDS             complement(1697..2572)
                     /locus_tag="PK288_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPM19131.1"
                     /translation="MKKITRRQFIGRSALGIGSAVVASHIPTGLHARSLPKSVRYPLG
                     FQVWTIKDQLIKDFAGTLKMMADMGYKSVEMCSPPGYESSGFGPLMKMTGKEMRKIMN
                     DLGLQFESTHYGMNELRNNLDERIQFASESGQKQMILSSFGLPKNATMNDWMKAADEL
                     NKIGERTKKAGIQTGFHNHHGEFEKIDGVLIYDALLKQFDPELVKMQFQVAVINIGIK
                     AADYFYKYPGRFISAHLADYSVLDQKSVPLGKGIVDWKEFFEATGKGGVKNIFVEMDP
                     ATFKDSAQFIQNLQY"
     gene            2910..4199
                     /locus_tag="PK288_09155"
     CDS             2910..4199
                     /locus_tag="PK288_09155"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HPM19132.1"
                     /translation="MKKCCSSFLRIVLSLQALLCFQGTIPSYGQAIDILLKGGHVIDP
                     ASNIDAPMDVAIVKDKIFRVAPDIPAENSAKVIDVRGMYVTPGLIDMHVHAFHGTDMG
                     SYIADGWDALPPDGFTFRAGVTTVVDAGSAGWRNFRKFKEQTIDRSQTRILAFLNIVG
                     SGMYGRFEEQDVNDMNPVMVAYMINRLFPGIIVGVKSAHYWGPDFTQVDRAVEAGRLA
                     NVPVMVDLGEHHPPLPIEELFMKHLRPGDIWTHTYANAPQDREVPVDENGKVKPFIFE
                     AQKRGIIFDVGHGGGAFHWKQAIPSVQQGFIADVISSDLHTGSMNSGMKDMANLLSKF
                     LNMGLPLQDVIKRATLNPAKAIKRTDLGTLGEGSVADIAVFSLREGSFGFLDVRGTKM
                     QGTQKLEAELTIRSGKIVWDLNGIGSPAWDAMPSASK"
     gene            4280..5545
                     /locus_tag="PK288_09160"
     CDS             4280..5545
                     /locus_tag="PK288_09160"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HPM19133.1"
                     /translation="MKSNTPAYFRKAVPAFLFIFCLILPALSQQADILLKGGHVIDPK
                     NGIDSQMDIEIAGGKIIRVAPDISSQDAKRTVDVKGLYVTPGLIDIHTHVFVGSGQGF
                     ADGFSSVSPDDFTFKAGITTVADAGTSGWRNFPVFRKQVIDRSQTRVLAFLNIAGNGM
                     TGFPSEEDINDMDTRMTSLVAEQYPDIIVGIKIGHYRGNEWTPFDRASEAALIAGVPL
                     LVECHLPFLPLEEILKRMRPGDIYTHSFCVASDRECLLDEKGKIKPFVAEAKKRGVRF
                     DVGHGGGMFHFDVAIPALKQGLIPDSFGSDLHRSSMNSGMKNMLDIMSKYFNMGMTFE
                     DIIFRATWNPAQSLRRDDLGHLSEGSVADIAVFSIRKGTFGFVDTGGTRLNGDRRIET
                     ELTIRGGKIVYDQNGISATGRIDSQGNRF"
     gene            5584..7191
                     /locus_tag="PK288_09165"
     CDS             5584..7191
                     /locus_tag="PK288_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPM19134.1"
                     /translation="MERRDIIRSLTILPFAGGFRPIESILSPVTGPLEPGQNIYQSIG
                     VEPVINCVGTYTIIGGSIERPEVVEAMHAASGFFVQYDELALGIGRRLAELTKAEWGM
                     ISSGCAAGMKHVTSACIAGGNPEKLVRIPDLTGFDKTEVVIPRSSRNAYDHSIRNIGV
                     TIITVDTLEELGKALNARTAMIYVMTGRDTLPGNKFAIDKIAALAKPLNIPFLVDAAA
                     EDLTIPNVHLQAGATVVAYSGGKAICGPQCAGLLLGQKDILMSAWQASSPHHGTGRDN
                     KVGKEEMLGMLAAVEAWVKRDHEAKYRTWLGYLDVISKRVSAVSGVTVTLGEPADLNN
                     RSPWLIISWDPARLNVAGPEIAEMLAQTQPRIAVHSGSNEQDKTTFLRVTSGQMQPGN
                     DKIVADRIFELLSKKYPEPKEMTAPSGDISGRWTVDIQFYSSKSQHTFILEQDGNWIQ
                     GSHKGDFSFREMVGTIEGDQVKLSSSERMIADNVPFIFSGTLSGDTITGQIYMGEYIN
                     ASFTANRNNYRGTRRQIRFPKGQPLAT"
     gene            7275..>8522
                     /locus_tag="PK288_09170"
     CDS             7275..>8522
                     /locus_tag="PK288_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPM19135.1"
                     /translation="MKRRDFIRVSSAAGVMGLINPVYGTGSMSGMVDDQGFDLHPFIK
                     EHPEAVFINLTSVSAPNDKQAIHDAAFRLAGEIMVRTSGGEGFPLSTRITCKPNWTCT
                     GRIDPKNPDSQLGITTDVNFIEGFLKAVKNLGPEKFYLRECACPHLWNAAGYTAMAER
                     NGFDLRDLSSKDFWDLPEGDIIFRKVDGVVFKEVGFMAPMTAQDTFLINIAKLKSHQM
                     GITGAIKNLQGMTGRKFHQFCGGHYDIFKTYDERYHKFFQPDYMDKIAELQKKHEEQG
                     IPRWRSRQERPPYGGGMFMEQWVQRMIDAYSVAPTGINIVEGIYGRDGDGFSGGPHNG
                     KAMNYMSNNIIFGKDAFRVDIIAHWLAGHEPGNFGLFHIGIERGVSNVLNPFDIPVYL
                     WEDGKAKKVSLDTLKRTPLLTLYM"
BASE COUNT         2339 a         1811 c         2016 g         2356 t
ORIGIN      
        1 ctttttccgg ggtattttga aaaatttcct gtgattgcca gttgtaccct gtagtttgag
       61 aatttctgaa gtatttctcc tgcaaacctg gttttcagat caaagaagtc tttatttaat
      121 gatgattcat tgactatcat agcattacag tcatgctgtc ctatttcagc tattaaatca
      181 agaatatctc cgacttcggc gatctttact ttattatctg tcagttctgc aataatcttt
      241 cctgacgaag atttgtgata ttttatcatc cggctaatcc ttttgttaaa ccacaaagtg
      301 taatacctta gagaactcag gaacatcaac tataacaact tctgcccttc ctgccacccc
      361 ttaccagggg ggcgctcaga ttcaaaagtc ccccttgggg cctgcctgcc gaagcgacag
      421 cgcaggcagg gatttagggg tgagttccgt atcgctgcta ttcagacggg aatatcttgt
      481 ttacgtattt tgttctgatc gccttttcaa atactttcca gccttcagca gtcatccggt
      541 cgggtgtaaa taaacagatc ccggcggcac cgttttccat tgattcccgt attgctgctt
      601 ccagttctcc cggaacaagg ccgtggttct caggatcctt ctcagcagct ttcttttccg
      661 gttcaggaca gatgaacagc ccgctataaa cgggtttgga attgtttatc gttgttacag
      721 cttcttttga tactgcacct atccattccg gtccttcaag ataaaagtcg ttgtaattca
      781 tcgggaagaa agcatcaatg tcccatttat cccattcctg ccttactatt tttttcgcaa
      841 ttgagttagg accggggaaa acagcagcat ttatctgttt gtttttggca tggaccatga
      901 cagccagccg gttgaccatg tcagttatca ggtcataccg gaattgtttc cattcctgta
      961 cagatgaagg atcaggtacc gactttatat caatgcctgt ttttgtcttg aaatctgcca
     1021 cacatttatc gcaataacaa tagtcatact gaggaaactc cctgtccata acaagaccgt
     1081 atttttccca gagtccccgg gcaagtataa catcaggaaa tcttataaaa tcgagatgga
     1141 tgccatcgac ttccggtaaa tcggcaatct tgccatacat gttttcgagg aattgataga
     1201 cttcttcacg gttgggacac aaaaatttgt aatgatccac ataagcaggc ttgtcataag
     1261 ccgactcgcc aaggccattg actgcataca attcaggtct gagttcaggc cggggttgct
     1321 gtacaagtgt tggtatccag gtatgaaatt ccatcccggc ttcctttgcc agcttaccga
     1381 cccttttgta agtcagggga tcctgtccgc cggaatacat taatccatca atacctttgt
     1441 ccttcaaaaa tttgaatttt gctgcaagat cagcatcagt ggcttctccc ggaccgctca
     1501 gccaggcgta acaagggatt tttgtttctt cggtaacgct gcatgatata aacaaacata
     1561 atgtaacgaa tgtcaaaagt tttttcattg taattttttt ttgataacgg atgaccgact
     1621 cctgacgccc gataccattt gacggatacc ggacgtcgta cgccggacgc cggataccgg
     1681 gcgctgtacg ccggatttaa tattgtagat tctgaatgaa ctgtgcacta tctttgaacg
     1741 tcgcaggatc catttcaaca aagatgttct ttactccgcc cttacctgtt gcctcaaaga
     1801 attctttcca gtcaacaatt cctttgccga gtggaacact cttctggtcg agtaccgaat
     1861 aatcagccag gtgtgcagaa atgaaccttc ccggatattt gtaaaaataa tccgctgctt
     1921 taatacctat atttataact gcaacctgga actgcatttt cacaagctcg ggatcgaact
     1981 gtttcagaag agcatcataa ataaggacac catctatttt ttcaaattca ccgtgatgat
     2041 tatgaaatcc ggtctggata ccggcttttt ttgttctttc tcctatttta ttcaattcat
     2101 cagcagcttt catccagtca ttcatagtgg cattcttcgg cagaccgaag cttgacagga
     2161 tcatctgctt ctgtcccgat tctgaagcaa attgtatccg ttcatccagg ttgttcctga
     2221 gctcattcat gccataatgg gtgctttcaa actgcagacc aaggtcattc attattttgc
     2281 gcatctcttt tcccgtcatt ttcatgagcg gaccgaatcc tgaactttca tatccgggag
     2341 gggaacacat ctcaacactc ttatacccca tgtcggccat catcttaaga gttccggcaa
     2401 aatccttaat aagctggtcc tttattgtcc agacctggaa accaagagga tacctgacag
     2461 attttggtag tgaacgggca tgaagaccgg tgggaatatg cgaagccaca acagctgagc
     2521 ctatcccaag agcgcttctg cctataaact gtcgtcttgt tatctttttc ataataattg
     2581 agttattaat ggttcattga aaataccttt ttaagttctg catgtacccg gctgctgaaa
     2641 aaacggtgtg aaaatcctga tctacccccc catcccccca aggggggctt tgtaagcatc
     2701 agtaattcat taagtccccc ctgggggatt taggggtcaa aggtcaccag gaggattttc
     2761 acacagcttc taaaacagtt agcactgtgt ataaccgtaa tgtaaaaata taaaatctgt
     2821 ttaattatgt tatctttatt gctcaaatgt tttgcttaca tgcaaatcat cgctgtccgg
     2881 tgtgattagc ggaaataaac tttttacaaa tgaaaaaatg ctgttcatca ttcttaagga
     2941 tagttctgtc gcttcaggct ttattgtgtt ttcagggtac gattccgtct tatggacaag
     3001 ccattgatat actcctgaag ggagggcatg ttatcgatcc cgcatccaat attgatgctc
     3061 ctatggatgt agccatagtt aaggataaga tattccgggt agctcccgat attcctgctg
     3121 aaaattcagc aaaggtcata gatgtaaggg gaatgtatgt tactcccggg ctcattgaca
     3181 tgcatgtgca tgctttccac ggcactgata tgggatcata tattgccgat ggctgggatg
     3241 cacttcctcc tgacggattt acattccgtg caggggttac aactgttgtc gatgccggat
     3301 cagccggttg gcggaatttc aggaaattca aggaacagac aattgaccgg tcgcaaacaa
     3361 gaatacttgc gtttcttaat attgttggat cggggatgta cgggaggttt gaggagcagg
     3421 atgttaatga tatgaatccg gtgatggtgg cctacatgat caacagattg tttccgggta
     3481 ttattgttgg tgtcaagtcg gcacattact gggggccgga ttttacacag gtcgatcgtg
     3541 cagtcgaagc aggcaggctg gcaaatgttc ctgtaatggt tgatctcggg gaacatcatc
     3601 caccattgcc tattgaggaa ctttttatga aacacctcag gccgggagat atctggacac
     3661 acacttatgc aaatgcacca caggatcgtg aggttccggt ggacgaaaat ggtaaggtga
     3721 agcctttcat ttttgaagca cagaaacgag ggataatttt cgatgtcggc cacggaggcg
     3781 gagcctttca ctggaaacag gctattccct cggtacagca gggatttatt gctgatgtga
     3841 taagctccga tctgcatacc ggcagcatga acagcggaat gaaagatatg gctaatcttc
     3901 tgtctaaatt cctgaatatg ggtcttccgc ttcaggatgt aattaaacgt gccactctga
     3961 accctgcaaa agctatcaaa aggactgacc tgggaaccct tggtgaagga tcagtagctg
     4021 atatagcagt tttcagcctc cgggagggca gtttcggttt cctcgatgtc cgcggaacaa
     4081 aaatgcaggg cactcagaaa cttgaagctg aacttacaat ccgctcggga aaaattgtat
     4141 gggacctcaa cggaatcgga tctccggcct gggatgccat gccttcagct tcaaagtgaa
     4201 ggttactttt acacaggtta cagaaattaa aattcaggaa gcaattttta tcatgtcaga
     4261 accagcggta aaaaggacaa tgaaaagtaa tacccctgct tatttcagga aagctgttcc
     4321 cgctttttta ttcattttct gtttaatctt accagccctt tcccagcagg cagatatcct
     4381 tcttaaaggc ggtcatgtga ttgatccgaa gaatggtatt gattcacaga tggatatcga
     4441 aatagccgga ggaaaaataa tcagggtggc tccggatata agttcacagg atgcaaaaag
     4501 aactgttgat gtgaaaggtc tgtatgttac tccgggcctc atagatattc atacacatgt
     4561 gtttgtagga tccggtcagg gctttgctga cggattttca agcgtttcac cggatgactt
     4621 tacatttaag gcaggcatta ccaccgttgc agatgcaggt acctcgggat ggaggaattt
     4681 cccggttttc aggaaacagg ttattgacag atcacagaca agggtccttg catttctgaa
     4741 tattgcggga aatggtatga ccggtttccc ttcagaagag gatattaatg acatggatac
     4801 acggatgact tctcttgtag cagagcaata ccccgatata atagtcggca taaagatcgg
     4861 tcattacagg ggaaatgaat ggacgccgtt tgacagagct tctgaagcag cacttattgc
     4921 cggagttcct ctgcttgtcg aatgccacct tcccttcctt cctcttgaag aaatacttaa
     4981 aaggatgaga ccgggagata tatatactca ttcattttgt gtggcttccg accgtgaatg
     5041 cctgcttgac gaaaagggga aaatcaagcc ttttgtagcc gaagcaaaga agagaggagt
     5101 ccggtttgat gtgggtcacg gaggagggat gttccatttc gatgtggcaa tacctgcatt
     5161 gaaacaggga ctgatccctg attccttcgg atcggatctt caccgttcca gcatgaattc
     5221 gggaatgaag aatatgctcg atataatgtc gaaatacttt aatatgggta tgacttttga
     5281 ggatataatc ttcagggcta cctggaatcc ggcacaatcg ctcaggcggg atgatctcgg
     5341 acatttaagt gaaggatctg tagctgacat tgctgttttc agcataagaa aaggtacctt
     5401 tggattcgtt gatacaggtg gtaccaggct gaacggggac cgcaggatag aaacggagct
     5461 tacaatccgc ggagggaaga tagtttatga tcagaatggc atttccgcga caggcaggat
     5521 tgacagtcag ggaaacagat tttgaagaaa tggaacagat attttaacta aagaacaaga
     5581 agtatggaac gaagagacat aatcaggagt ctgacaattc tgccatttgc cggaggcttt
     5641 cgcccaattg agtcaatatt atctccggtt acaggtcctc tggaaccggg ccagaatatt
     5701 taccagtcga taggagtgga accggtcata aactgtgttg gcacatacac aattattggc
     5761 ggttccattg aaaggcctga agttgttgaa gccatgcatg ccgcatcggg cttctttgtt
     5821 cagtatgatg agctggccct cggcatcgga aggaggctcg cggagctgac taaagcagag
     5881 tgggggatga tctcatcggg ttgtgccgcc gggatgaaac atgttacttc cgcctgcatc
     5941 gcaggtggca atcccgagaa acttgtacgt atccctgatc ttacaggttt tgacaagacc
     6001 gaggttgtaa ttcccagaag ctcaagaaat gcctatgatc attcaatcag gaacatcgga
     6061 gttacaatta ttactgttga tacactcgaa gagcttggga aggccctgaa tgcgaggaca
     6121 gcaatgatat acgttatgac cggtcgtgat actcttccgg gtaataagtt cgcaatagat
     6181 aagattgccg cgcttgcaaa accattgaac ataccatttc ttgttgatgc agccgccgaa
     6241 gatcttacaa tacctaatgt tcatctccag gcaggggcaa cggtggtggc ttacagcgga
     6301 ggtaaggcaa tttgcgggcc tcagtgtgcc ggactgcttc tcggacagaa ggacatattg
     6361 atgtcggcat ggcaggccag ctccccgcat cacggcaccg gacgcgacaa taaggtcgga
     6421 aaagaagaaa tgcttggaat gctggcggca gtcgaagcct gggtcaaacg tgatcatgag
     6481 gcgaaataca ggacatggct gggatatctt gatgtaatat caaaacgtgt ttcagctgta
     6541 agcggagtta cagtaaccct gggggagcct gctgacctta ataaccggag cccatggtta
     6601 attatttcat gggatcctgc aaggcttaat gttgcagggc ctgaaatagc tgaaatgctt
     6661 gcacaaacac aacccaggat tgctgttcac agcgggagta atgaacagga caaaacaact
     6721 tttctaaggg taacatccgg gcagatgcaa ccgggaaatg ataagatcgt tgctgacaga
     6781 attttcgaac tgctgtcgaa gaaataccct gaacccaaag aaatgactgc accctcagga
     6841 gatatttcag gaagatggac tgtcgatatc cagttttaca gcagcaagag ccagcatact
     6901 tttattcttg aacaggatgg caactggata cagggatcac ataaaggcga tttttcattc
     6961 cgtgaaatgg tcggtacaat cgagggtgac caggtgaaac tgagcagctc agaaagaatg
     7021 atagccgata atgtgccctt catattctcg ggaaccctct cgggagatac aattaccggc
     7081 cagatctata tgggggaata tataaatgcc agttttacgg caaaccgcaa taactacagg
     7141 ggaacaagaa gacagataag atttcccaaa ggtcagccgc ttgcgacctg atatatgatt
     7201 aattgtaaac cttattgtca tttagatgtt taatagcagg attaattagt ttaaaaaaca
     7261 atagaagaat ataaatgaaa agacgtgatt ttataagagt ctcttctgca gcaggagtta
     7321 tgggacttat taatccggta tatggtaccg gttcaatgag cgggatggtg gatgatcagg
     7381 gttttgatct gcatcctttt ataaaggaac accccgaagc tgttttcatt aatctgacat
     7441 cggtctctgc tcccaatgac aaacaggcca tacatgatgc tgcgttcagg cttgcaggag
     7501 aaataatggt gagaactagt gggggggaag gttttccgtt atcaacgagg atcacctgta
     7561 agcccaactg gacatgtacc gggaggatcg atccgaaaaa tcctgattct caacttggaa
     7621 ttactacaga tgttaatttt atcgaaggtt tcctgaaggc tgtaaaaaat ctgggcccgg
     7681 agaaattcta tctcagggaa tgcgcctgtc cgcatctgtg gaatgcagca ggatatactg
     7741 ccatggccga gagaaatggg tttgaccttc gggatcttag ctcaaaagac ttctgggacc
     7801 tgcctgaagg tgatataata ttccggaagg ttgacggagt tgtctttaag gaagtcggtt
     7861 ttatggctcc aatgactgcc caggatacct tccttattaa tattgccaag ctcaaatccc
     7921 accagatggg aattacgggt gctataaaga accttcaggg catgacagga aggaaatttc
     7981 atcagttctg cggaggacat tacgatattt tcaagaccta tgacgagcgt tatcataagt
     8041 tcttccagcc cgattatatg gataagatag cggagcttca gaaaaagcac gaagaacagg
     8101 gaattcccag gtggaggagc aggcaggaga ggcctcccta tggtggcgga atgttcatgg
     8161 aacagtgggt acagaggatg atcgatgctt acagtgttgc tccgaccggg ataaatattg
     8221 tagagggtat ttatggccgc gacggcgatg gtttttccgg aggtccccat aacggcaaag
     8281 ccatgaatta tatgagcaat aatattattt tcgggaaaga tgcattcagg gtggatataa
     8341 ttgctcactg gcttgccggt cacgaacccg gaaatttcgg actattccat atcggtatcg
     8401 aacgtggtgt ttccaatgtg cttaatccct tcgatattcc tgtgtacctc tgggaagacg
     8461 gaaaggcaaa gaaagtaagc cttgatactc tgaagcggac acctcttctc actctgtata
     8521 tg
//
LOCUS       DAOOYG010000113         8520 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9212_length_8520_cov_5.078323, whole genome shotgun sequence.
ACCESSION   DAOOYG010000113 DAOOYG010000000
VERSION     DAOOYG010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9212_length_8520_cov_5.078323"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK288_09175"
     CDS             <1..315
                     /locus_tag="PK288_09175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM19136.1"
                     /translation="GFVIQGGCPYSKDPGDPRTGTGGPGYTIRCELTGANQYHDRGVL
                     SMAHAGRDTGGSQFFICRNRANTRHLDRQHTCFGKVTEGLDVIDLIQQGDRIDKIVIL
                     GE"
     gene            317..694
                     /locus_tag="PK288_09180"
     CDS             317..694
                     /locus_tag="PK288_09180"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HPM19137.1"
                     /translation="MAFEISGRVIDIAPVNQVSDKFRKREFVIEKKEAGGAGLFIDYI
                     KFQLIQDKCDLINESFLNAEVKVTFNLRGNKWEREGKVSYFTNLDAWKIERIRTEDTE
                     QEFPPHITDESFPGDDQLDDLPF"
     gene            848..3523
                     /locus_tag="PK288_09185"
     CDS             848..3523
                     /locus_tag="PK288_09185"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HPM19138.1"
                     /translation="MKCISLVLLAAVFTVSSLKAQVLHETVSEGIRERIQHSYNRFIQ
                     PAGLQVVFGNEALENHAMDVALSPDARYVAVMERYSIVFISTADNKVRYSLVNNEHPL
                     LRGGMNTYSGLKWDEDASGMHVYWSVVGADNRSFVASATWNGEKAEFSRLIEYKTMPP
                     AAIALPNEILITREAGNKYLYVVLNGNNTLVKQDFITGDTVWYTFPGVAPYGIAAAAG
                     KVYVTNWAGRFPTDRDKAVAGVPWGLARVDNKAGGSTREGSVAVFDAETGTFLKDIIV
                     GLHPNGIISGRKGRYIYVTNSNSDNVSVINTSRDVVTETISVRLQPDINPYFGDSPNG
                     ICLSPDEQTLYVANGMDNALAVISLGKKAGNRQGGRESTVTGFIPTGAYPSGICITPS
                     GSVYVANLESDGVHVGIKNPAGENLIYNSHHLLASISVFPAPGSRDLAAYTDTVIAVN
                     NLARATTAREEPRSGVAPKPVPERIGEPSLFRHVLYIIKENRTYDQILGDMKEGRGDP
                     SLCTYGELITPNTHKLAREFLLLDNFNVSGKCSAEGHQWTDAAIVTDYIEKNMRAWFR
                     SYPHVQDDALVYSPSGFLWDNGVNRGKKVRIYGEASYPVFENNTSPGWTEIYNKFLRG
                     EKVEFTNYTTIEPVKSLLSPTYPSYGNHEFPDVMRAADFIRELKEYEAMEGDQLPELM
                     IMALPNDHTAGLRPGHPTPRAMITDNDLALGQIVEAVTKSRFWESTVIFVTEDDSQGG
                     WDHISANRTVGLVISPYSRLRTTIHTYYSQPSMIRTIEQILGLPPMNIQDAIAYPMTD
                     CFGSEPDLTPYTALPNNIPLDEMNKPLSSLSGRALHFARKSMLPEFAGVDSGNDDLLN
                     RILWFAARGNAPYPRKYAGATGYDDGDDDPDGDGY"
     gene            3465..5579
                     /locus_tag="PK288_09190"
     CDS             3465..5579
                     /locus_tag="PK288_09190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPM19139.1"
                     /translation="MQEQPATTMATTTLTVTDIDNAANYPNMKNPLLDKWSTPYETPP
                     FHLIEVSHFKPAAEEAIKAALAEVEAIADNPGKPAFENTVGALERTGEKLSRISSLLF
                     SLNSAETSDELQNAAQEISPMLVRFSNDVTMNGKLFERIHALYESKGESGLDTEQMML
                     LEKVHRNFILGGAGLDPEHRKRFREISEELAELTLRFEANVLHQTNSFRLHITDEKDL
                     AGLPPDIVAMASAEAEKKEKTGWVFTLNQPSYIPFMQFAANRDLREKMFRAYGSRCFH
                     DDEKDNSGLVLRIVNLRLEKAKLLGFQSYAHMALTDRMADSPEKVNAFLERLNKASRD
                     AALRDYENLKSFAIEQGLKYPLERWDWPYYSEKLKRARYNIDDEILKPYFRLESVRDA
                     IFGLASGLFGLQFVQNTDIPVYHGEVSAWEVHDDDGKILAILYLDHHPRKGKHGGAWM
                     TSFRNQRSENGKRIIPHVSIVMNFTRPGPAKPSLLAFNELTTYLHEFGHALHGILSDC
                     TYESLAGTNVAHDFVELPSQFMENWAYEKEWLDTWAVHYETGEKLPGELIEKIRETAV
                     FNEGYACNRQLGFGFLDMAWHTVSEPVSKSVDEFERETMTATEILRPVGGTNMSCSFT
                     HLFGGEYAAGYYGYKWAEVLDADAFYFFRESGLFNKTISGSFRKNILERGGSDKPIDL
                     YIRFRGKEPSIEPFLDRSGLKK"
     gene            5643..>8520
                     /gene="purL"
                     /locus_tag="PK288_09195"
                     /gene_synonym="purI"
     CDS             5643..>8520
                     /gene="purL"
                     /locus_tag="PK288_09195"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPM19140.1"
                     /translation="MIRFFRKNSGAIIAVQSDVRLTDSDSEKLAWLFGNATMLDDDRL
                     EGVYTGPRREMITPWSTTAVEITQNMGIVGIKRIEEFIPTRERKPEYDPMLQALYRDP
                     DQDIFTVIRDPEKIMPVIDIRGYNEKEGLALSNEEILYLEKLSRKLSRPLTDSEVFGF
                     SQINSEHCRHKIFNGIFIIDGEEKDSTLFQLIKNTTHFNRNRVVSAYKDNCAFLKGPV
                     VEQFAPATHDRPDYYTVKKYESVLSLKAETHNFPTTVEPFYGASTGSGGEIRDRMAGG
                     RGAFPEAGIAVYMTSYPRIEGGQPWEKAIKERPWLYHSPADILTKASNGASDFGNKFG
                     QPLICGSLFTFEHSENMQVWGYDKVIMLAGGIGNGKSSQSEKGVPGKGDLIVMLGGDN
                     YRIGMGGGAVSSVDTGEFDSSIELNAVQRANPEMQKRVYNAIRALCESDDNPVISIHD
                     HGAGGHLNSLAELVEATGGRIDISRLPVGDPTLSAREIIGNESQERMGLIISRANADK
                     LVNISEREKAPLYFIGEITGDHRLVFENSITGEKPIDLSVEALFGDPPRTIITGSSPS
                     GKAFERVEYDRNRIHEYVELVLQLEEVACKDWLTNKVDRSVTGRIAKQQCAGPLQLPL
                     NNLGAIALDYRGRSGFATSLGHAPAAALIDPAAGSVLSIAEALTNIIWAPLTDNLKSV
                     SLSANWMWPCKNPGEDARLYSAVQAASRFAIELGINIPTGKDSLSMTQKYSDIVVMSP
                     GTVIITAAGEVNDIRRIVEPVIVDDPFTSLLYIDMSRDEFRIGGSSLAQVLNRVGDDA
                     PTVRDAGYFARVFNTIQGLIKKGRILAGHDISAGGMITTLLEMCFSNISGGIAANLTA
                     LNEPDTVKILLSQNPGIIIQVADENEVSEILLEHGISYFTIGHPVSDRTISIVNHGET
                     LRFEIDPLRHRWFRTSYLFDRKQCGPRAALERFINYRNHILRFNL"
BASE COUNT         2351 a         2025 c         2207 g         1937 t
ORIGIN      
        1 ggatttgtta tccagggagg ctgcccgtat tcgaaggatc cgggcgatcc caggacaggt
       61 acaggcggac cgggatacac catcaggtgt gaactcaccg gtgccaacca gtaccacgac
      121 aggggtgtgt tgtcaatggc ccatgcgggc agagataccg gtggatcgca attcttcata
      181 tgccgcaaca gggcaaacac caggcatctc gaccggcagc atacatgttt cggaaaagta
      241 acggagggcc ttgatgtaat tgacctgatt cagcagggcg acaggattga caaaatagta
      301 atactcggag agtgatatgg cgtttgaaat ttccggcagg gttattgata tagcccccgt
      361 caaccaggtg agcgacaaat tccggaaacg cgagtttgtc attgaaaaga aggaggcggg
      421 aggggcaggc ctgttcatcg attatatcaa gttccagctt attcaggata aatgcgatct
      481 tataaatgaa tccttcctta atgccgaagt gaaggtaacc tttaacctga ggggaaacaa
      541 atgggaacgc gaaggaaagg tgagttactt caccaatctc gatgcatgga agattgaaag
      601 gatcaggacc gaggatacag aacaggaatt tccgccgcac ataaccgacg aatcatttcc
      661 aggtgatgat cagctcgatg atcttccctt ctgaaggaaa ttattttttc cgttaacatt
      721 ctgaattcat atatttgcag ggatgtcatg taaacatccc tgttttgcgt tgatgcattt
      781 ttcactgcat tctggctgtg agtattaggc aacgcatcgg gacccggttc acttaactgt
      841 tgcaatcatg aaatgcatat cattggtatt actggcagca gtgttcactg tcagttcatt
      901 aaaagcccag gttcttcatg aaacagtcag tgaaggtatc agggaaagaa ttcagcattc
      961 atacaaccgc tttatacaac ctgccgggct gcaggttgtt ttcggaaatg aagcgcttga
     1021 gaaccatgcc atggacgttg ccctttcacc cgatgcccgg tatgtggcgg taatggaacg
     1081 ctacagcata gttttcataa gcactgcgga caataaggtc agatacagcc ttgtcaacaa
     1141 tgagcatccc ctcctgaggg gcggaatgaa cacatattcc ggcctgaaat gggatgaaga
     1201 tgcgtcgggg atgcatgttt actggagcgt ggtcggtgca gataacaggt catttgtggc
     1261 ctctgcaacc tggaacggtg aaaaggctga attctcccgc cttattgaat acaagaccat
     1321 gccgccggca gctattgcac tgcccaacga gatacttatc accagggagg ccggcaacaa
     1381 atatctttat gttgtcctga acggaaacaa tactctggtc aaacaggatt tcatcacagg
     1441 cgacacggtg tggtatactt tccccggggt cgctccgtac gggattgcag cagcagccgg
     1501 aaaggtatat gtaaccaact gggcaggacg ctttccaacc gacagggaca aggctgtagc
     1561 cggggttccg tggggccttg caagggttga caacaaggcc ggcggctcca cacgcgaagg
     1621 aagcgtcgca gtatttgatg ctgaaacggg aactttcctg aaagatataa ttgtagggct
     1681 ccaccccaac ggaataatca gcggcagaaa gggcaggtac atctatgtaa ccaactccaa
     1741 cagcgataat gtgtcggtaa taaatacatc aagggacgtg gttacagaaa caatcagcgt
     1801 caggcttcag cccgacatca atccgtattt tggcgattcc ccaaacggca tatgcctttc
     1861 acccgatgaa cagacccttt atgttgcaaa cggaatggac aatgccctgg ctgtgatttc
     1921 gctcggcaaa aaggccggta acaggcaggg cggcagggaa agtactgtaa caggatttat
     1981 ccccacaggg gcctatccat cgggaatctg catcacacct tcaggatcgg tttatgtggc
     2041 caatctggaa tctgacggag tccatgtagg cattaaaaat cctgccggtg agaatcttat
     2101 atacaactcc catcatctgc tagcatcgat ttctgttttt ccggccccgg gaagcaggga
     2161 tcttgctgcc tataccgata ctgttattgc agtgaacaac ctggcacgtg ccacaactgc
     2221 cagggaggaa cccagaagcg gggtcgctcc aaaacccgtg cccgaaagga tcggggaacc
     2281 ctccctgttc aggcatgtgc tttacatcat caaggaaaac cgcacttacg accagatcct
     2341 gggcgacatg aaggaaggac gcggtgatcc gagcctctgc acttacggag aactgatcac
     2401 tcccaatacc cacaagctcg ccagggaatt ccttcttctc gataatttca atgtgtcggg
     2461 gaaatgctcg gcggagggac atcagtggac agatgcggca attgtgacgg attacataga
     2521 aaagaatatg cgggcctggt tcagaagcta tccgcacgtc caggacgatg cactggtata
     2581 ttctccaagc ggcttcctgt gggataacgg ggtgaaccgc gggaaaaagg tgagaatata
     2641 cggcgaagcc tcatatcccg tatttgaaaa caataccagt cccggatgga cagagatata
     2701 taataaattc ctcaggggag aaaaagtgga atttacaaat tataccacaa ttgaacctgt
     2761 aaaaagcctt ctcagcccca cttacccctc gtacggtaat catgaatttc ctgatgtgat
     2821 gagagctgct gattttatca gggaactgaa ggaatatgaa gccatggaag gcgaccagct
     2881 gcccgaactg atgatcatgg cccttcccaa tgaccatact gccgggctga gacccggaca
     2941 tcccaccccg agagccatga ttaccgacaa cgaccttgcc ctcggacaga tagtagaagc
     3001 tgtcaccaaa agcagattct gggaaagcac tgttattttt gtaacagaag atgattccca
     3061 gggaggctgg gatcatattt ccgcaaaccg gacagtcgga cttgtgatca gcccctactc
     3121 ccggctcagg accacgatcc atacatatta cagccagccg tcaatgataa ggacaattga
     3181 acagatactc ggccttccgc cgatgaatat tcaggatgcc attgcttatc cgatgactga
     3241 ttgttttggc agcgaacccg accttacacc ctacactgcc cttccaaata atattccgct
     3301 tgatgagatg aataaaccgc tgtcatcgct ctcaggcagg gcgctccatt ttgcaaggaa
     3361 gagcatgctt cccgagttcg caggcgttga ctcgggaaat gatgacctgc ttaacaggat
     3421 attatggttt gctgccaggg ggaacgctcc ctatccccgg aaatatgcag gagcaaccgg
     3481 ctacgacgat ggcgacgacg accctgacgg tgacggatat tgataatgca gccaattacc
     3541 cgaatatgaa aaatcctctt ctggataaat ggtccacacc ctatgaaaca cctccctttc
     3601 accttattga agtctctcat ttcaaacctg cagccgaaga ggccataaaa gcagcacttg
     3661 ccgaggtgga ggccatcgcg gacaatcccg ggaagcctgc atttgaaaat actgtcgggg
     3721 cccttgaaag gacaggggaa aaactctcca ggatatcttc cctgctgttc agccttaaca
     3781 gcgccgaaac atcggatgaa ctgcagaatg cagcacagga aatatcgccc atgctggtgc
     3841 ggttttccaa tgatgtaacc atgaacggta agctgtttga gaggatacat gcactgtacg
     3901 aatcaaaggg ggaatcaggg ctggataccg aacagatgat gctgcttgaa aaagtacacc
     3961 ggaattttat tctcggcggg gccggtcttg atcctgagca ccggaagcgg ttcagggaaa
     4021 tctccgaaga actggcggaa ctaaccctca ggttcgaagc caatgtgctt caccagacaa
     4081 actcattcag gctgcatatt acggatgaaa aggatcttgc cggcctgccg cctgatattg
     4141 tggccatggc atcggcagaa gcggaaaaga aagaaaaaac aggatgggta ttcacactga
     4201 atcagccaag ctacataccc ttcatgcaat ttgccgccaa ccgggatctc agggagaaaa
     4261 tgttccgggc ttacggatcc aggtgctttc acgacgatga aaaagataac agcggccttg
     4321 ttctgagaat tgtaaatcta aggcttgaaa aagcaaaact gctcggtttt caaagctatg
     4381 cacacatggc cctcaccgac cgtatggcag acagtcccga aaaggtaaat gctttccttg
     4441 aaaggctcaa taaggcctcg cgcgatgcag ctctcaggga ttatgagaat cttaaatcat
     4501 ttgccattga acagggattg aaatacccgc tcgagcgatg ggactggccg tactattcag
     4561 agaagctgaa aagggcaagg tacaatatag acgacgagat cctgaaaccg tatttccgtc
     4621 tcgaatctgt aagggatgct attttcggtc ttgcgtccgg cctgttcggg ctgcagtttg
     4681 tgcagaacac tgatatcccc gtctatcacg gggaagtcag tgcctgggag gttcatgacg
     4741 atgacggaaa gatccttgca attctctatc tggatcatca tcccagaaaa ggcaagcacg
     4801 gaggggcctg gatgacatca ttcaggaacc agaggtcgga aaacgggaaa aggatcatcc
     4861 cgcatgtttc cattgtgatg aactttacaa ggccgggacc ggcaaagcct tcgctcctgg
     4921 cattcaatga gctcacaacc tatcttcatg agtttggcca tgcccttcat gggatcttgt
     4981 cggattgcac atatgaaagc cttgccggga ccaatgtcgc ccacgatttt gtcgaattgc
     5041 cgtcgcagtt tatggaaaac tgggcgtatg agaaagaatg gctcgacacg tgggccgttc
     5101 attatgaaac cggtgaaaag cttcccggag agcttataga aaagatacgg gaaaccgcag
     5161 tattcaatga aggatatgca tgcaacaggc agctgggatt cggttttctc gacatggcat
     5221 ggcacacggt aagcgagccc gtcagcaagt cagttgatga gtttgaaagg gaaaccatga
     5281 cggcaacgga aatactccgg ccggtcgggg gaaccaatat gagctgctct ttcacccatc
     5341 ttttcggagg tgaatatgcg gcaggctatt acggttacaa gtgggctgag gtgcttgatg
     5401 ctgatgcatt ctattttttc cgcgaatcgg gcctgttcaa caaaactatc tccggctcat
     5461 tccggaaaaa tatccttgaa aggggaggca gcgacaagcc aatagatctg tatataagat
     5521 tcaggggtaa ggaaccttca attgaacctt ttcttgaccg cagcggcctg aaaaaataat
     5581 attatatttg ccagttcaat tttcaatcgt cctttgatcc cttaactttt aataaccatc
     5641 ccatgatccg ttttttcaga aaaaattcag gagccattat tgccgtgcaa agtgatgtca
     5701 ggttaaccga ttctgacagt gagaaactgg cctggctgtt cggaaatgcc acaatgcttg
     5761 atgatgacag gttggagggc gtgtataccg gaccccggag ggagatgatc acaccgtgga
     5821 gcacaactgc tgtggagata acacagaaca tgggaatagt gggaataaaa aggatcgagg
     5881 aattcattcc cacgcgtgag aggaaaccgg aatatgatcc catgctgcag gcattataca
     5941 gggatcctga ccaggatatt ttcacggtca tcagggatcc ggaaaaaatc atgcctgtta
     6001 ttgacatcag ggggtataat gaaaaggaag gtcttgccct cagcaatgag gaaattcttt
     6061 accttgagaa gctgtctcgg aaattatcca gaccccttac cgacagtgag gttttcggtt
     6121 tttcacagat aaactcggaa cattgcaggc ataagatctt caatggaatt ttcattatcg
     6181 acggggaaga gaaggattcg acactattcc agcttataaa gaataccact catttcaacc
     6241 gcaacagggt ggtttctgcc tataaggaca actgtgcctt tctgaagggt ccggttgtgg
     6301 agcagtttgc acctgccacc cacgacaggc ctgattatta tactgtaaag aaatatgaat
     6361 cagttctttc gctaaaggcc gaaacccata acttccccac caccgttgag cccttctatg
     6421 gtgcttccac aggatcgggg ggtgagatcc gcgaccgcat ggccggaggc aggggtgcct
     6481 ttcctgaagc cgggatcgcg gtctacatga cttcatatcc ccggatcgaa ggagggcagc
     6541 catgggaaaa agccattaag gaaaggccct ggctctatca ttccccggcc gacattctca
     6601 ccaaggcctc aaacggggca agcgatttcg gtaataagtt cggccagcct cttatctgcg
     6661 gatcgctttt tacttttgaa cattccgaaa acatgcaggt atggggttat gataaggtca
     6721 tcatgcttgc agggggaatc gggaacggga aaagcagcca gagtgaaaaa ggtgttcccg
     6781 ggaaaggaga tcttatcgtc atgctgggcg gagacaacta caggatagga atgggaggag
     6841 gagccgtatc gtcagtcgat accggggaat tcgacagttc catcgagctc aatgccgtac
     6901 aaagggcaaa ccccgaaatg cagaaaaggg tttacaatgc aataagggcc ctgtgcgaat
     6961 cggacgacaa tcctgtcatt tccattcatg atcacggggc aggcggccat ctcaacagcc
     7021 tggccgaact ggtggaagca accggcggga gaatagacat cagcaggctg cctgtcggcg
     7081 atccaacact gtcggcacgg gagattatag ggaacgagtc gcaggaacga atgggtttga
     7141 taatcagtcg ggccaatgca gacaaattgg taaatatttc cgaaagggaa aaagcaccgt
     7201 tatatttcat aggggagata accggcgatc acaggctggt ttttgagaac agcataaccg
     7261 gcgaaaaacc cattgatctt tccgttgagg cattgttcgg tgatccgcca aggacaatta
     7321 taacgggcag cagtccttcc ggaaaagcct ttgagagggt tgaatacgac aggaaccgta
     7381 tacatgagta tgtggaactg gtactgcagc ttgaagaggt tgcctgcaag gactggctca
     7441 ccaacaaggt ggacaggtcg gtaaccggcc gtattgcaaa acagcagtgt gccgggccgc
     7501 tgcagctgcc cctgaacaat ctcggggcaa tagcactgga ttatcgtggc cgttccggtt
     7561 ttgccacatc actggggcat gcccctgccg ccgcgcttat tgatcccgca gccggatcag
     7621 tgctgtctat cgcagaagct cttaccaata ttatatgggc tccccttacc gacaacctga
     7681 aaagtgtatc cctttcggca aactggatgt ggccctgcaa aaatccggga gaagatgcca
     7741 ggctgtattc agcggtacag gctgccagcc ggtttgccat tgaactcgga ataaatattc
     7801 cgaccggtaa ggacagcctt tccatgactc agaaatacag cgacattgtg gtaatgtcac
     7861 cgggaacggt aataatcact gctgcgggag aggttaatga catccggaga attgttgaac
     7921 ccgtgatagt cgacgatccc tttaccagtc tactttacat tgatatgagc agggatgagt
     7981 tcaggattgg cggaagcagt ctcgcacagg tgctgaaccg tgtaggggat gatgctccga
     8041 cggtgaggga tgccggctat tttgccaggg ttttcaatac tatccaggga ctgatcaaaa
     8101 aaggcaggat actcgcagga catgatatat ctgcaggcgg aatgataaca acgttactgg
     8161 agatgtgctt ttccaatatc tccggcggca ttgcggcaaa tctgactgcc ctcaatgaac
     8221 ccgacactgt aaagatactg ctgagccaga acccgggaat aattatccag gtggccgacg
     8281 aaaacgaggt ttccgaaatc cttcttgagc atggcatatc ctatttcacc atcggccatc
     8341 cggtcagcga cagaacaatt agtatcgtca atcacggcga gacgctgaga tttgaaattg
     8401 atcccctcag gcacagatgg ttccgtacct cctatctctt tgacagaaag cagtgcggtc
     8461 ccagggcggc tctcgaaaga ttcatcaatt acaggaacca cattctccgg ttcaacctgg
//
LOCUS       DAOOYG010000114         8510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9223_length_8510_cov_6.948433, whole genome shotgun sequence.
ACCESSION   DAOOYG010000114 DAOOYG010000000
VERSION     DAOOYG010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8510
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9223_length_8510_cov_6.948433"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="PK288_09200"
     CDS             <1..1761
                     /locus_tag="PK288_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPM19141.1"
                     /translation="PVWEGITFATMDQYRTLWEGEYNGGLPLIFNFMNNLYGMGGQTM
                     GETMGFGILARVGAGVNPEMMHAERIDGYNPLAVIDAFRRKRKILEEKRGPVLIDTLT
                     YRISGHSPSDASSYRSREEIEAWQKEDSVNAFGTMLTDSGIADPGMLKQIREEVGELM
                     VKILRLSIDDTISPHLDLDGDPDGIGKMMFSNCSLDAMEQGIPQVYHPMEENPRVKSL
                     KNKERFYRDREGNPVSKARLYQLRDGIFEAVIDRFYRDPTLAAWGEENRDWGGAFAVY
                     RGLTEALPYHRLFNSPISEGAIVGTAIGYGMCGGRVIAEIMYCDFLGRSGDEVFNQLP
                     KWQAMSGNLIRMPVVIRVSVGSKYGAQHSQDWSSLVAHIPGLKVVFPATPYDAKGLMN
                     SALQGTDPVIFFESQRIYDIGEMFHEGGVPEGYYEIPLGEPDVKMEGSDITILSVGAT
                     LYTVVKAAETLKKNYGLNAEIIDARSLVPFNYEPVVRSVMKTGKIVLASDASSRGSFL
                     KDMAQTITELCFDHLDAPPVVVGSRNWITPAFEMERYFFPQPGWIIDAINEKIVPLKG
                     HKPGTDQSDQKTLELNSRGI"
     gene            1923..2708
                     /locus_tag="PK288_09205"
     CDS             1923..2708
                     /locus_tag="PK288_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPM19142.1"
                     /translation="MENIAEKIRNKAGFIIDMDGVIYHGNKLLPGVVEFLRWLEETGK
                     SYLFLTNASQRTPKELHEKLERLGINVGEEHFYTSALATAGFLASQKPDGSAYIIGDA
                     GLIHALYSIGYTTNNVNPDYVVVGDTSSYNFEKIETAVNLVLRGARLIGTNPDVSGPV
                     ETGISPSVKALIAPIEIASGKKAYFVGKPNPLMMRIALKKLGVKREEAIVIGDRMDTD
                     IRCGLESEIDTLLVLSGITGRGDIDNFPYRPRYVLNGIIDLIK"
     gene            2717..3355
                     /locus_tag="PK288_09210"
     CDS             2717..3355
                     /locus_tag="PK288_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPM19143.1"
                     /translation="MKETVEYKDERKEVARFMRRLYRQRLTTTSGGNISLRISNDLIL
                     ITPSATDKGRMRWKEVGIMSILGENLSSELKPSIESGMHLAIYRKRKDVLAIIHAHPV
                     IASGFTATGMDIRTDLTAEARAICGNPVMVPYALMGTNDLASRAADSIGDSSILLLEN
                     HGILTTGATLIEAFDRLEVLENAAIMTLMTEMTGKRKPLGKMRLREIERLFR"
     gene            3352..3996
                     /locus_tag="PK288_09215"
     CDS             3352..3996
                     /locus_tag="PK288_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPM19144.1"
                     /translation="MIKGVLFDMDGVLVDSESFICRAAIMMFKEIGLDVLPDDFIPFV
                     GTGENSYLGGVAGKYGKPIEMEKAKARTYEIFGDLARGQLKALPGALELIKDCLGKGL
                     RISLATSADRVKMEISLNEIDLPPSTFNTIITGLDVARKKPYPDIYIKAAESLGLEPS
                     GCLVVEDSVSGITAGKAAGCKCLAVTTSFSPAELTEADWICSSLLQVPDDALNW"
     gene            4043..5041
                     /locus_tag="PK288_09220"
     CDS             4043..5041
                     /locus_tag="PK288_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016267744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPM19145.1"
                     /translation="MKYNKLGKSDVEVTELAFGAWAIGGWLWGGADSDDAVKAIETAV
                     DHGMTTIDTAAVYGFGLSEELVGKAVKGKRNKVQILTKFGMIWDMKKGKFYFSTRDSE
                     GKEIDIYNLSSRERVISDCEASLKRLGTDYIDLYQIHWPDATTPVSETMEALDTLIRQ
                     GKIRAGAVSNYQVDLMQEALRTLQIASNQVPYSMVNRSIEKEIIPFCLENNIGILAYS
                     PMQRGLLAGKIKKGHKFGEGDNRADTIYYREPNLSRIISLTEDLGVIAAERDVTLAQL
                     VLNWTIRQPGITCTLAGARNPAQVLENIKAVEFTLTDDELNRINALVAGLKIETDI"
     gene            5048..6292
                     /locus_tag="PK288_09225"
     CDS             5048..6292
                     /locus_tag="PK288_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19146.1"
                     /translation="MKNFLASFPSYGELNKAMNGMTVPEYREANAHIHTPYSFSAFPD
                     LDTIFRMAKNEKVDVLGINDFNVADGYEQFHRGCLKNGIFPLFNIEFIGLMKDEQKKG
                     IRINDPNNPGRIYFCGKGLDYPFHAGFINRIRLRKIRKESQNQIRSMIAKLNSLIEKH
                     DPQIKLSYESVRQEHARNLVRERHLAKAVRMVAERHYPDPEKRISFIESLYGGKKSKA
                     GPDDHSSLENEIRSNLLKSGGAAFVEEDEKSFLPLDKIMKIIIDAGGIPCYPVLLDDS
                     AGNFTEFESDPEKLHDSLREKGIECIELIPMRNDAKHLNRFVEYFQERGFIITFGTEH
                     NTPDLAPLTVSTRGAVPLSENLKKTAWEGACLLAAHQYLRADGRQGYVLPDGTSNREQ
                     RNNMAALGQMVIEYYFNKFNHD"
     gene            6285..8258
                     /locus_tag="PK288_09230"
     CDS             6285..8258
                     /locus_tag="PK288_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM19147.1"
                     /translation="MTDEIRDLLEISRYYGRDKNYVIAGGGNSSFKDDRTIWIKASGQ
                     ALSDLDETGLVALSREKIRAISLKKYPDDPAEREECVKRDLQSAITGTGSPKRPSVET
                     SLHELIGYRFVVHLHPTSVNGIMCSRNAGMLTAKLFGEKALFVPYTDPGYTLFKKLES
                     DLESYREKHEEDPTMIFLENHGVFVSGNTVDGIRSAYDKIISAILNYLGPLPPASEIS
                     FNPVLHKVLPVMRMLFAEKNPKIIRYRHNTLIEKYYQNQKEFHRISLPLTPDIIVYCK
                     TRYLYVENSATPEKIIDSLKQQLPAFRKEYGYMPKIVVIKDAGIFAVEDSVSAAETVL
                     DVYEDMIRIVSLSSEAGGTKTLTPEQVAFIDQWEVENYRRKIALAGKEQGKLNNRVAV
                     ITGGAQGFGAGIAESLFEHSVNVVVADLNEETGMKLVSSLNARKSAGRALFVRTDVAS
                     PDSVKDLVAITVREFGGIDIMISNAGILRAGGLDETDPGDFEKTTSVNYNGYFHCAKY
                     ASEIMKLQNREKPDYFTDIIQINSKSGLRGSNRNFAYSGGKFGGIGLTQSFALELAPF
                     RIKVNAVCPGNFYEGPLWSDPGNGLFVQYLRTGKVPGAKTIDDVRKFYEEQVPMKRGC
                     RLVDVVKAVLYIIDQEYETGQAVPVTGGQVMLG"
BASE COUNT         2460 a         1886 c         2237 g         1927 t
ORIGIN      
        1 cccgtatggg aagggatcac ttttgcaacc atggaccagt acagaacgct atgggagggt
       61 gagtacaatg ggggattgcc ccttatcttc aatttcatga acaacctgta cggaatgggt
      121 ggccagacca tgggtgaaac aatgggcttc ggcatccttg ccagggtagg tgcgggagtg
      181 aatcctgaaa tgatgcatgc tgaaaggata gacggataca atccccttgc agtgatagat
      241 gccttccgcc gtaagaggaa gatcctggag gaaaaaagag gcccggtgct catcgataca
      301 ctaacatacc ggatcagcgg tcattctcct tccgacgcat catcatacag gtcaagggag
      361 gaaattgaag catggcagaa ggaggattct gtcaacgcct tcggaacaat gctgactgat
      421 tccgggatag cggatccggg gatgcttaaa cagatcaggg aagaggttgg ggaattgatg
      481 gttaaaatac tcaggctgag catcgatgat acgatatctc ctcacctgga ccttgacggt
      541 gatcctgacg gaatcggaaa gatgatgttc tccaactgtt ctctggacgc aatggaacag
      601 ggtatccccc aggtatatca cccaatggag gagaatccca gggtgaaatc acttaagaac
      661 aaggaaagat tctacaggga cagggaagga aatccggttt caaaagccag gctctaccag
      721 ctgagagacg ggattttcga agcagttatc gaccgctttt acagggatcc gaccctggca
      781 gcctggggag aggagaatcg cgactgggga ggagcttttg ccgtttaccg ggggctcacc
      841 gaggccctgc cttaccacag gcttttcaac tcacccatat cagagggtgc aatagtcgga
      901 acggcaatag gctacggcat gtgcggagga agagtaatag cggagataat gtactgtgat
      961 ttccttggca gatcgggtga tgaagttttt aatcagctgc caaagtggca ggccatgagc
     1021 ggcaacctca tcaggatgcc cgttgtaata agggtttctg tgggttccaa atacggtgcc
     1081 cagcattcac aggactggtc atcgctggtg gcccacatac cgggcctcaa ggtggttttc
     1141 ccggcaactc catacgatgc aaaaggattg atgaacagcg cactgcaggg aaccgacccg
     1201 gtcatcttct ttgaaagcca gaggatctac gacatcggag aaatgttcca tgaaggcggc
     1261 gtaccggagg gttattacga aatcccgctt ggcgaaccgg atgtcaagat ggaaggaagc
     1321 gacatcacaa tcctttcagt gggagccact ctgtatactg ttgttaaggc agctgaaaca
     1381 ctgaagaaaa actatggtct caatgccgaa ataattgacg ccagatcgct cgtacccttt
     1441 aattatgaac ctgtcgtccg atcggtaatg aaaaccggaa aaatcgtgct tgccagcgat
     1501 gcatcttcac gcggctcctt cctgaaggat atggcccaga caatcactga actctgcttc
     1561 gatcacctcg acgcaccacc tgtggtggtc ggttcaagaa actggatcac gccggccttt
     1621 gaaatggaaa gatatttctt tccgcagccc ggctggatca tcgatgcaat caatgaaaaa
     1681 atagttccgc ttaaaggtca caaacccggc accgatcagt cggatcaaaa aacccttgaa
     1741 ctgaacagca ggggaattta gcagacagtg catactgaac ccccgaagct gtcttttgta
     1801 attttcagga tgaaatccgg gaaagagacc ttttccgtga aagtactggc ggaaaaatct
     1861 gtttgctgaa ccgtcagggg gtgtggaacc tttttgatat atttggagca caattaagaa
     1921 ccatggaaaa cattgcagaa aaaatccgga acaaagccgg ctttataata gatatggatg
     1981 gggtcatcta tcacggtaac aagttgctgc ccggagttgt tgaattcctc aggtggctcg
     2041 aggaaactgg caaaagctat ctgttcctta ccaatgccag tcagaggacc ccgaaagagc
     2101 tgcacgagaa gctggaaagg ctgggaataa atgttggcga agagcacttt tacacaagtg
     2161 cccttgctac tgccggattc ctggcaagcc agaaacccga tggaagtgca tatataatcg
     2221 gggatgccgg gcttattcat gccctgtaca gtattggata tacaacgaat aatgtaaacc
     2281 ccgactatgt ggtggtgggc gataccagca gctacaactt tgaaaagata gaaacggccg
     2341 ttaacctggt gttaagggga gccaggctga taggaacaaa tcccgatgtg agcggtccgg
     2401 ttgagaccgg aatttccccc tcggtgaagg cccttattgc tccgatagag atcgcatcgg
     2461 gaaagaaggc ctattttgtc ggaaaaccaa atcctctcat gatgaggatc gccctgaaaa
     2521 agcttggagt caaaagggag gaagcaatag tgatcggaga ccggatggat accgacataa
     2581 ggtgcgggct ggagtcagaa attgatacac tgctggtgtt aagcggcatc accggtcgcg
     2641 gtgatattga caatttcccc taccgtccgc gatatgtact caacggaata atcgatctga
     2701 tcaagtgatc cggcttatga aagagactgt tgaatataaa gatgaaagaa aagaagttgc
     2761 caggttcatg cggcgcctgt atcgccaacg gctgacaaca acttccggtg ggaatatcag
     2821 tctcaggatc tcaaatgatc tgatcctcat cactccgtcg gcaaccgata aaggcaggat
     2881 gcgctggaaa gaggttggaa taatgtctat cctgggtgaa aatctcagtt ctgagcttaa
     2941 gccttcaatt gaatcgggta tgcatcttgc aatttacaga aagagaaagg atgttctggc
     3001 aattattcat gcccacccgg tcattgcatc gggatttacg gcaacgggaa tggacatcag
     3061 aacggatctg acagcagaag ccagggcaat ctgcggtaac ccggtaatgg taccatacgc
     3121 tcttatggga acgaatgatc tggcctccag ggctgctgac tcaataggag attcatcaat
     3181 actgctcctg gagaaccacg ggatcctgac aaccggggct acccttattg aagctttcga
     3241 cagactggag gtcctggaaa atgccgcaat aatgacatta atgacagaaa tgaccggtaa
     3301 aaggaaaccg cttggaaaaa tgaggcttcg tgaaattgaa agacttttca gatgataaag
     3361 ggagtgctct ttgatatgga tggggttctg gtcgactcgg aatcgtttat ttgcagggcg
     3421 gccataatga tgttcaagga gattgggctg gatgttttac cggacgactt catccccttt
     3481 gtgggcacag gtgagaacag ctacctggga ggtgtggctg gaaaatatgg gaaacccatt
     3541 gaaatggaga aagcaaaggc ccgtacctat gaaatatttg gtgatcttgc cagggggcaa
     3601 ctcaaagcgc tgcccggagc actggagctt attaaagact gcctcgggaa ggggctcagg
     3661 atatccctgg ccaccagtgc cgacagggtg aaaatggaga tcagtcttaa cgagatcgat
     3721 ttgcctcctt ccactttcaa cacaatcatc acaggcctgg atgttgcaag gaaaaagcca
     3781 tatcctgaca tatacattaa ggcggcggag agcctgggac tcgaaccatc cgggtgtctt
     3841 gtcgttgagg attctgttag cggcataaca gcaggtaaag ccgcgggatg taaatgcctt
     3901 gccgttacta catctttttc tcccgcagag cttactgaag ccgactggat ttgcagttcc
     3961 ctccttcagg tgccggatga tgcactgaac tggtagtttc tgaataaaaa tgttaaacct
     4021 gcaataacca aacttattga atatgaagta caacaaactt ggcaaatcag atgtggaggt
     4081 caccgaactg gctttcgggg cctgggcaat aggaggatgg ctctggggcg gagccgattc
     4141 agatgatgca gtaaaggcaa ttgaaaccgc tgtcgaccat ggcatgacaa ccatcgacac
     4201 tgccgccgtt tacggcttcg gcctcagcga ggaacttgta ggtaaagctg tgaagggaaa
     4261 aagaaacaag gttcagatcc tgaccaaatt cggcatgata tgggacatga aaaaaggaaa
     4321 gttctacttt tccaccaggg atagcgaggg gaaggaaatt gacatttaca acctgtcgtc
     4381 ccgcgaaagg gttatctctg attgcgaagc cagcctgaaa agactcggca ctgactatat
     4441 cgacctttac cagatacact ggcccgatgc cacaacgccg gtgtcagaga caatggaagc
     4501 gctcgatacc cttattcgtc agggaaagat cagggccgga gccgtttcaa actatcaggt
     4561 cgacctgatg caggaagctc tcagaaccct gcagattgca tccaaccagg ttccctacag
     4621 tatggttaac cggagcattg aaaaggaaat aattccgttc tgccttgaaa ataatatagg
     4681 gatccttgcc tacagtccga tgcagagggg attgcttgcc ggtaaaataa aaaagggcca
     4741 caaattcggg gaaggcgaca accgggccga cactatatat tacagggaac ccaatctgtc
     4801 aaggattatc agcctgacag aggatctcgg agtcattgcc gcagaaaggg atgtcaccct
     4861 ggcacagctg gtactcaact ggacgatccg gcagcccgga atcacctgca cactcgcggg
     4921 cgcaagaaat ccggcacagg tactggaaaa tatcaaagcc gtggagttca cccttacgga
     4981 tgatgaactg aaccggatca atgccctcgt ggccggacta aaaattgaaa ccgacatata
     5041 agccagtatg aagaattttc ttgcttcctt cccttcatac ggggagctga acaaggcaat
     5101 gaatggcatg accgtccccg agtatcgaga ggctaatgcg catattcaca caccgtattc
     5161 attcagtgcc tttcccgatc tggataccat tttcagaatg gccaaaaacg aaaaagtcga
     5221 tgtgctggga atcaacgatt tcaatgtcgc tgacggatat gaacagtttc acaggggctg
     5281 tctgaaaaac gggatattcc ccctgttcaa cattgaattc attggtttaa tgaaagatga
     5341 gcagaaaaaa gggataagga tcaatgaccc caataatccc ggcaggatct atttctgcgg
     5401 caaaggtctc gactatccgt tccacgcggg tttcataaac aggatacgcc tgagaaaaat
     5461 aagaaaagaa agccaaaacc agatcagatc aatgatagca aagctcaatt cattaattga
     5521 gaaacacgat ccgcaaatta aactcagtta tgaatcagta agacaggagc atgcccgtaa
     5581 tctggtgaga gaaaggcatc tggccaaagc agtcaggatg gttgccgaaa gacactatcc
     5641 cgatcctgaa aaacgaatct cattcattga atcactttac ggcgggaaaa aatcgaaagc
     5701 tggcccggat gatcattcat cactcgaaaa tgagataagg tcaaacctgc ttaaaagcgg
     5761 aggtgcagca tttgtggagg aagacgaaaa atcattcctt ccgcttgaca aaataatgaa
     5821 gatcatcatc gatgccggag gcattccctg ttatccggtc ctgctcgatg acagtgccgg
     5881 gaattttacc gaatttgaat ctgacccgga aaagcttcac gattccctga gggaaaaagg
     5941 cattgagtgc attgaactga ttccaatgag aaacgatgca aaacatctta accggttcgt
     6001 tgaatatttt caagagcggg gctttataat tactttcgga acggaacaca atacccctga
     6061 ccttgctccg cttacggtaa gcaccagggg agcggttccg ctgagcgaga atttaaagaa
     6121 aacagcctgg gaaggagctt gcctgctggc cgctcaccag taccttaggg ccgacggacg
     6181 tcagggatac gtgcttcctg acggtacctc caacagggag caaaggaata atatggctgc
     6241 cctgggacag atggtcattg aatattattt caataagttc aatcatgact gatgaaataa
     6301 gggatctact tgagatttcc cggtattacg gtagggacaa gaattatgta attgcgggcg
     6361 gcggaaacag ttcctttaaa gatgaccgca caatctggat aaaggccagc ggacaggctc
     6421 tttcagatct tgacgagaca ggcctcgttg ccttaagcag ggaaaagata cgtgcaatat
     6481 cgctaaaaaa gtaccccgat gatcctgcag aaagggaaga gtgtgtcaag cgcgatctgc
     6541 aatcagcgat aacaggaacg ggatctccca aaaggccgtc agtggaaact tcgctgcatg
     6601 agctgatagg atacaggttt gtcgtccacc tccatcccac ctccgtcaac ggaattatgt
     6661 gcagcaggaa tgcaggaatg ctcactgcaa aacttttcgg ggaaaaagct ttgttcgtac
     6721 catataccga tccgggttac acgcttttca aaaagctgga atcggatctt gagtcttaca
     6781 gggaaaagca tgaagaagat cccaccatga tctttctcga aaatcacggt gtgtttgtgt
     6841 cgggaaatac tgttgacggg atcaggtcag cgtatgataa aatcatttcg gcaatcctga
     6901 attacctggg acccctgccc ccggccagtg agatctcctt taatccggtg cttcacaagg
     6961 tattgcctgt aatgaggatg ctgttcgcag agaaaaaccc gaaaataatc aggtacaggc
     7021 acaacaccct gattgaaaaa tactatcaga atcagaaaga attccacagg atctcactcc
     7081 ctcttacacc cgatatcatt gtctattgca agacaagata cctttatgtg gagaattccg
     7141 caacccctga aaagatcatt gattccctga aacagcaatt gccggctttc aggaaggaat
     7201 atggatacat gccgaaaatt gtggtgataa aggatgcagg aatatttgcg gttgaagata
     7261 gcgtatccgc ggctgaaacg gtcctggatg tgtatgagga catgatcagg atcgtcagtc
     7321 tatcgtcgga agcaggcggc acaaaaacgc tgacccctga gcaggttgct tttattgatc
     7381 agtgggaagt tgagaattac aggagaaaga ttgccctggc aggcaaagag cagggcaagc
     7441 tgaacaacag ggtggcggtg ataaccgggg gcgcccaggg attcggggcc gggattgccg
     7501 aatccctgtt tgagcattcc gtcaatgttg tggttgcaga cctgaatgaa gaaaccggca
     7561 tgaagctggt gtcatcgctc aatgcccgga aatctgccgg cagggcttta tttgtaagaa
     7621 ccgatgtggc aagtccggat tcggtaaaag accttgtagc cataaccgtc agggaattcg
     7681 gcggcatcga catcatgata agcaatgccg ggatcctcag ggcaggagga cttgatgaaa
     7741 ctgatcccgg agattttgaa aaaacaacct ctgtcaacta caatggctat tttcactgtg
     7801 ccaaatatgc ttcagaaatc atgaagctgc agaacaggga aaagccggat tatttcacgg
     7861 atattataca aatcaactcc aaatcgggtc ttcgcggtag taaccggaat tttgcctatt
     7921 caggtggtaa attcggaggg ataggtctta cccagtcgtt tgccctggag cttgctcctt
     7981 tcagaattaa ggttaacgcc gtttgtccgg gtaatttcta cgaggggcca ttgtggtccg
     8041 atcccgggaa cggcctgttt gtacaatatc tgagaacggg gaaggttccg ggtgcaaaaa
     8101 ccatcgatga tgtaaggaaa ttttatgagg agcaggtgcc gatgaaaagg ggctgcaggc
     8161 tggtggatgt ggtaaaggca gttctttata tcatcgacca ggaatatgag acagggcagg
     8221 ctgtaccggt aaccggaggc caggtgatgc tgggataggc tccctttgcc gccatggaga
     8281 tggcatgagg ctgcgcttat ctcttttatg ggacatcggg caatcttatt gcctgcggaa
     8341 agaatgattt ttatttaatg tattcattat gaaaaccaag gcagtaaggc tttacggaaa
     8401 aaaagatctt cgcctggaag aatttgaact tccggaaatg aaggacgatg agatacttgc
     8461 acaggtcata tcggacagca tctgcatgtc gtcacacaag gcagcgctgc
//
LOCUS       DAOOYG010000115         8493 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9250_length_8493_cov_6.220313, whole genome shotgun sequence.
ACCESSION   DAOOYG010000115 DAOOYG010000000
VERSION     DAOOYG010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9250_length_8493_cov_6.220313"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1050..1619)
                     /locus_tag="PK288_09235"
     CDS             complement(1050..1619)
                     /locus_tag="PK288_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19148.1"
                     /translation="MEEYNVPSPLTFNPLKHHLPYIRDFVNLNIQLTAEQGWNPSEVR
                     KMLRHLGTSVMDVYNGPLHLSQIFAEILDYLEVRKLLSGNSFASWAGTGFSSFRIISL
                     TDKSDWILKYNDNEKRYVHIFPAKKSPHTFRIKSNTLKSAILYLIMEGKDYVTRDDLN
                     RARAITGLPPVKNVEETRAVMEMIDILRI"
     gene            1761..3017
                     /locus_tag="PK288_09240"
     CDS             1761..3017
                     /locus_tag="PK288_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPM19149.1"
                     /translation="MGESSRQNFLKTINHQQPERVVVDFGSTAVTGIHVRVVEKLRDY
                     YGLAKKPVKVTEPYQMLGEVDEELIQAMDIDVIGLYGEKNMFGVPNRDWKQARTPWGQ
                     EVLFPGSFNYTINENGDILMYPEGDTTAGPSAIMPKSGFFFDALERQGPIDDSSLRLE
                     DNLEEFSFVTEHDIDYWKQQAAGVENSGKGIIASLGGTALGDIALVPAMQLKNPKGIR
                     GVSEWYISTLIREDFVKDIFDRQTDIAVENLKRIYGAIGNRIDAVFICGTDFGTQDST
                     FCSPETYKRVWMPYYRKINDWIHGNTGWKTFKHSCGAVESFMELFIESGFDIINPVQI
                     NAAGMDPVLLKKKYGNRIVFWGGGVDTQGAFAFGTPQQVREQVRRQCGILNENGGFVF
                     NSVHNIQANVPFENVVAMLETLRDLR"
     gene            3045..4439
                     /locus_tag="PK288_09245"
     CDS             3045..4439
                     /locus_tag="PK288_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HPM19150.1"
                     /translation="MHSKYNWGYLLIITAISALGGLLFGYDWVVIGGAKPFYEAYFGL
                     DGNVTLQGWAMSSAITGCLAGAALSGWLSDLFGRKKLLIVSALLFLLASFGTGSAGSY
                     SGFVTFRIVGGTGIGLASTLSPLYIAEITPANVRGKFVSINQLTIVIGILAAQYVNWK
                     IARPVPADATVLEIMTSWNGQFGWRMMFYACAVPAGLFFILMWFIPESPRFLAKGRCN
                     RMKVFHILHRIGGDEYAGKELESILTTLYQDEKRIDFKLFKNPGFRKLLIMGVVIAVF
                     QQWCGINVIFNYAHEIFANAGYSVSDTLFNIVITGSVNLVFTLVGMFTVDKAGRKALM
                     LLGAAGLAGIFATVGTMYYLHIQGIPLLIMVVAAIACYAMSLAPVTWVVLSEIFPNRI
                     RGFAMAVCTFALWSACFILTYTFPILNKFMKASGTFWLYGIICVLGFWFILRKLPETK
                     GKTLENIEHEFVDQ"
     gene            complement(4433..5449)
                     /locus_tag="PK288_09250"
     CDS             complement(4433..5449)
                     /locus_tag="PK288_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPM19151.1"
                     /translation="MKNLVIMKRLTMFLVCLNLALLARSQQLPVYSQYLYNKFLINPA
                     HAGSDGLTSYNITAREQWVGYAGAPRTFSVSGQTRILKKKYRLKQNIFDQTVYVPKTE
                     GRVGFGGYIFSDRNGLVQRTGFQTSYSYHLWLEDYTQISMGLAITGYHYKINVDETSF
                     RESNEPLLSDNLRRGVFIPDVDFGIYVLNPGYEVGFSVLQLAGAAARLGNNSNGNYWM
                     DRHYYLFGSFSLPSGAKAKLKPSTLIKISEQFRPQADIGLTYVYDTKFWMGMSYRTGS
                     SIVTNIGMKYVNSRIKMTSVYLGYSFDFAISKIQRVTYGTHEIVIALKLGDSARRFRW
                     KDRY"
     gene            complement(5451..>8493)
                     /locus_tag="PK288_09255"
     CDS             complement(5451..>8493)
                     /locus_tag="PK288_09255"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPM19152.1"
                     /translation="LSISKSSLQTSYSAAGDQVDYTIMVTNDGNVTLSDISVTDPVTG
                     LDQTIPSLIPGTSRTFPTTYIITQNDMNNGQVLNTTTASYTFNGNRYTESDYAIVRAD
                     IRPGVAISKSTAERYFTTAGDVIHYTIVVTNTGNVTLTGVTVSDPNTILSCPGAPFIL
                     APGRTVTCTANHTITVDDVNRGSFSNRASVSGLDPLMNRISETSNEVTVTLNNAPPVI
                     SCLRPITVNAPPTSCQVELTGGLAVTFSDPNNNISSVTWVMTGATNGRSLSSGINNLS
                     SYIFNGGVTTITYTVTDAPGLSATCSFTVTVNDLTDPVISCAGPRDRFTDTNGPDYTV
                     QGTEFDPLSADDNCAVAGLINDFNNATSLSGARFPVGTTNVTWTVTDGSGRTASCSFA
                     VTVTDNVPPAAICRNIDVYLDLNTGLVSINPEDVDGGSYDNTAVASLSIDINTFDCED
                     LGPNNIVLTVTDIYDNSSTCTAVVTVHYAVVPNPVVTPATDVICNNETINLVMTNNMP
                     VTTWAWTVNIPAEISGASGDNSGLNSSIVQTLENTGAVVHEVVYEITPTVYGKCKLPA
                     ISSSIWVNPEPVIGIHSNDTIVCHGESTLITVRNPNTTVRGQWMYDLVVEPETEVTGY
                     SAGGTFTMPADLSETLSNDDIIPHKVVYRFTPRIVPDDGGPVCAGKEVAITIWANPGI
                     RYNTEISNYNGFNVSCFEKSNGYIRIDPHPEMAPYTFRWTGPEGFISVAQNISGQVAG
                     HYEMTITDRYGCSAVDDFDLTEPGKLGMTIATSTSRDGNYNIDCYGGQNGTATIMAVN
                     NVGSVDYMWSDGYLGRTRSNLPAGTHKIIISDSNNCLADSTVTLTEPDEIKIEFIVTP
                     AFCPDKPDGEIGLIVTGGVSAGDYSYRWSDNSTEKSLSHVPEGSYSVTVTDFNGCNVT
                     GEIQIGSMHKICLVIPEAFSPNNDLINDTWVIDNADLYPQFEISVFNRWGQILWVSEK
                     GYPVQWDGRSRGEDLPVDSYHYVIDLHNGSRLIVGTVTIIK"
BASE COUNT         2266 a         1840 c         1983 g         2404 t
ORIGIN      
        1 gggatcactg ccacctgcgg ctgtgtaaaa tcatcggttt tatgcagccg ttgttattta
       61 ttgcatccac catgcaggag cgacgagaac gctcagggta gcggcgttcg aaccgggcga
      121 acaccgtgag cgagcttcag cgcttgcccg tgtcaaatgg caggggatcc ataattgctg
      181 ccagtttaaa ggtttatgca attccggacc ctaaaatccg cgcggttaaa ttgttcataa
      241 gtttttcctt atggttcata aaacattttg ccgacgagtc aattaactta attagatttt
      301 attattgtat ggttgtttca gttcactcat aagcgtataa aatgaagcgt gtaaagaaaa
      361 agctgcaact acccgctgac tatcattttt tactgcacct gtcagaattg ggccactgga
      421 aaaaactgca aagaacaatt gcggaaatag aagtcaaatc ccgcggcttt tatttatgtg
      481 tctatgaaga caaaaaaatt ctggtggttg agtagcttac ctgattgggg tccggaccgt
      541 taaacggacc aggtttcagg attaaccagg catcataaaa aacccggctg attgaatttt
      601 accgggtcgg gtgagcctgt tccgatgaaa aaagagaggc ggcaagatgc cacctccctc
      661 cgacaattaa aaacaaaaag agcaatctga cgacaggcgt taagcctgag aaggtttctg
      721 cttgattttc ccgtacataa taatgcttca attgtctttt atttcatttg aaaagccagc
      781 atccttaaaa attcattttt ctattggagt agaattgcga ctgagattac cgggacgctg
      841 gcttcatcat taactaaaaa ggtttttgca cttttattaa ttcattctta tcaaatttaa
      901 cattcgagtc agatctacgc aaattaattt gataatcatc ggtttatcat tgatgaacgt
      961 ggctcaatag tcaacaaagg ctaaaagggg atcccaactc cgggtgttta tgatacggca
     1021 tagaggaagg ataatctgac cggaaggaat caaatacgga gaatatcgat catttccatc
     1081 acggcccggg tttcttcaac atttttcacg ggcggtaaac ctgtgatcgc cctggccctg
     1141 ttcaggtcat ccctggtcac ataatccttc ccttccatta tcaggtacag aatggctgat
     1201 ttaagtgtat tggacttgat cctgaaggta tgcggacttt tctttgccgg aaaaatatgg
     1261 acatatcttt tttcattgtc attatatttt aatatccagt cggatttgtc ggtcagggaa
     1321 ataatcctga aactggaaaa accggttcct gcccatgatg caaacgaatt tcctgaaagc
     1381 agcttcctga cttcgagata atcgaggatt tcagcaaata tctggctgag atgaaggggt
     1441 ccgttataaa cgtccatcac cgatgttcca agatgcctca gcattttacg gacttcggaa
     1501 ggattccacc cctgctctgc ggtaagctgg atatttaaat tgacaaaatc cctgatataa
     1561 ggaagatgat gtttcaaggg attaaaagtc aacgggcttg gaacattgta ctcttccatc
     1621 cggatggaat tatgatcaat gataagttat taaaaaatca tgatattgga tcagcaaatc
     1681 ggtaaagtgc cttattcagt aggataatgg aaggaaactt ttaattttat aacctgtaat
     1741 ttccaaaaac ctgaaatatc atgggagaat catccaggca aaattttctc aaaaccatta
     1801 accaccagca gcctgaaagg gttgttgtcg attttggatc aactgcggta accggaattc
     1861 atgtcagggt agttgaaaag ctgcgggatt attatggttt ggcgaaaaag cctgtaaaag
     1921 tcacagagcc ataccagatg cttggtgagg tggatgaaga gctgatacaa gcgatggata
     1981 tcgatgttat cggtctttat ggtgaaaaaa atatgtttgg agttcccaac agggactgga
     2041 aacaggccag aaccccatgg ggtcaggaag tattgttccc cggcagcttt aactacacca
     2101 ttaatgagaa cggtgatatc ctgatgtatc cggaaggaga cacaacagcg gggccgagtg
     2161 cgattatgcc aaaatcggga ttcttttttg atgcattgga aaggcagggc cccattgacg
     2221 attcaagcct gcggctggag gataatcttg aagaattctc atttgttaca gaacatgata
     2281 ttgattactg gaaacagcag gcagcaggag ttgagaactc cggcaagggg ataattgcat
     2341 cacttggagg aacagcactt ggcgatatcg ccctcgttcc tgccatgcag ctgaaaaatc
     2401 caaaaggaat caggggtgta agcgaatggt atatttccac actgatcagg gaagattttg
     2461 ttaaggatat tttcgacaga cagaccgata tagcggttga gaatctgaag cgtatttacg
     2521 gtgccattgg aaacaggatt gatgcagttt tcatatgcgg aacggatttc ggaacacagg
     2581 attcaacctt ctgctcgcct gagacatata aaagagtgtg gatgccttat tacagaaaga
     2641 tcaatgactg gatacacgga aatactggct ggaaaacctt caagcactca tgcggggcgg
     2701 tagaatcgtt tatggagctt ttcatcgaat cgggatttga tattattaat cctgtccaga
     2761 taaatgcagc cgggatggat ccggtacttc tcaagaagaa atatggcaac cgtatcgttt
     2821 tctggggagg cggtgttgat acccagggtg cattcgcttt cggaacacct cagcaggtca
     2881 gggaacaggt taggagacag tgcggaatat taaatgagaa cgggggtttt gtcttcaatt
     2941 cggtacataa tatccaggca aatgtgccgt ttgaaaatgt tgtggcaatg cttgaaaccc
     3001 tcagggatct ccggtagaag cccggattat aatccgtact gatgatgcat tcaaaatata
     3061 attggggata tcttctgatt attaccgcta tttccgccct cggcggcctg cttttcggat
     3121 atgactgggt tgtaatagga ggggcaaagc cattctatga ggcatatttt ggccttgacg
     3181 gaaatgttac ccttcagggc tgggcaatga gcagtgcaat taccggctgt cttgccggcg
     3241 cagccttgtc aggctggctg agtgacctgt tcggtcgcaa aaaactgctc atagtatcag
     3301 cactgttgtt tttacttgca tcttttggaa ccggttctgc aggcagttat tcgggttttg
     3361 taaccttcag gatagtggga ggaacaggaa tcggactggc ttcaacacta tctcccttgt
     3421 atattgcaga gataacccct gcaaacgtga ggggaaagtt cgtgtcaata aaccagctga
     3481 cgatagtcat cgggatcctt gcagcacaat atgtaaactg gaagattgcc agacctgttc
     3541 cggctgatgc cactgttctg gaaataatga catcatggaa cggtcagttt ggatggcgga
     3601 tgatgttcta tgcctgcgct gttcccgcag gcttgttttt catccttatg tggtttatcc
     3661 ccgaaagccc cagattcctg gcaaagggca gatgcaacag gatgaaggta tttcatatcc
     3721 ttcacaggat cgggggcgat gaatatgcag gaaaagaact ggagtcgatc cttacaacct
     3781 tgtatcagga tgagaaaagg attgacttta aactgtttaa aaacccggga ttcagaaaat
     3841 tgctgatcat gggagttgtt attgccgtgt tccagcaatg gtgcgggatt aatgttattt
     3901 tcaattatgc acatgaaatt tttgccaatg ccggatacag cgtttcagat acacttttca
     3961 atattgtgat cacaggaagc gtgaatcttg tttttaccct ggtaggtatg tttactgttg
     4021 acaaggcagg ccgaaaagca ctgatgctcc tgggagctgc aggactggca ggtatttttg
     4081 ctactgtcgg tacaatgtat tatctgcata tccagggcat tccgctcctc attatggtgg
     4141 ttgccgccat tgcctgctat gcaatgtcgc ttgccccggt tacctgggtg gtcctttctg
     4201 aaatattccc caacaggatc aggggatttg caatggcagt atgcacattt gccctctgga
     4261 gcgcatgttt catacttacc tatacatttc ccatactgaa caaatttatg aaagcgtcag
     4321 gtacattctg gctgtacggg atcatatgtg tcctgggatt ttggttcata ttgagaaaat
     4381 tgcccgaaac caaaggaaaa acactcgaaa acatagaaca cgaatttgtg gatcaataac
     4441 gatccttcca cctgaacctc ctggcactgt caccaagttt cagggctatt acaatttcat
     4501 gggttccgta agtcactctc tggatcttgc ttattgcaaa gtcgaaggaa tagcccaggt
     4561 aaacggaagt cattttgatc cggctgttga catatttcat ccctatgttg gtgactattg
     4621 aactcccggt cctgtaagac attcccatcc agaattttgt atcataaaca taagtcaatc
     4681 caatatctgc ctgaggtctg aactgttcgg atatttttat cagggtggaa ggctttaatt
     4741 ttgcttttgc tcccgatggc agactaaaag agccaaaaag gtaataatgc ctgtccatcc
     4801 agtagtttcc gtttgaattg tttcccagcc tggcagcggc tcccgcaagc tgcaagactg
     4861 agaatcctac ttcgtatccg ggattgagaa cataaatacc gaaatccaca tccggaataa
     4921 aaactccccg gcgaagattg tccgacagca atggctcatt tgattccctg aagctcgttt
     4981 catccacatt gatcttgtag tggtagccag tgatagccag ccccatcgat atctgtgtgt
     5041 aatcctcaag ccacagatga taggaatagg aggtctgaaa gccggtcctt tgtaccaggc
     5101 cattcctgtc gctgaatatg tatccgccaa atccaaccct gccctcggtt ttgggaacat
     5161 acactgtctg atcaaatata ttttgcttta accggtactt tttcttaagg atcctggttt
     5221 gcccgctgac cgagaaagtt cttggtgctc cggcataacc tacccactgt tccctggctg
     5281 ttatgttgta acttgtcagt ccgtcgcttc ctgcatgagc cggatttatc agaaacttat
     5341 tatacagata ctgactgtat accggtaact gttgtgacct ggccagtagg gccaaattaa
     5401 gacagacaag aaacattgtc agccttttca ttataaccag attcttcatt ttactttatt
     5461 atggttactg tgccgacaat cagcctggac ccgttatgaa gatcaatcac ataatggtag
     5521 gaatcaacgg gcaggtcttc ccctctgctt cttccgtccc attgaaccgg ataacccttt
     5581 tctgataccc aaaggatctg tccccatctg ttaaatacag aaatctcaaa ctggggatac
     5641 aggtcggcat tgtcaatgac ccatgtgtca ttgatcaggt cgttattcgg tgaaaaagct
     5701 tcgggaataa cgaggcatat tttgtgcatt gagccaatct gaatctcacc tgtgacattg
     5761 cagccattga aatcggtaac ggttacacta tatgatcctt cggggacatg actcaggctc
     5821 ttttcggttg aattgtctga ccaacgatag gaatagtccc cggctgagac ccctccggtg
     5881 acaatgaggc cgatttctcc atcaggtttg tcaggacaaa atgcaggagt cacgataaac
     5941 tcaattttta tctcatcagg ttctgtaagc gttaccgtgg aatctgcaag acagttgttt
     6001 gagtcagaaa taattatctt gtgagtaccg gccggcagat tggacctggt cctgccaagg
     6061 taaccatctg accacatata atccactgat cccacattat tcaccgccat aattgtggct
     6121 gtaccgttct gtcccccgta acagtcaata ttgtaattcc catcacggct ggttgaggtt
     6181 gcaattgtca tacccagctt tccgggttca gtcaggtcaa aatcatcaac ggctgaacac
     6241 ccgtacctgt cggttattgt catttcataa tggcccgcta cctggcctga aatgttttgt
     6301 gcaacagaga taaaaccttc aggaccggtc catcggaatg tatatggagc catttcggga
     6361 tgaggatcta ttctgatgta tccgtttgat ttttcaaaac aactgacatt gaatccattg
     6421 taatttgata tttctgtatt gtatcttatc cccggattgg cccaaatggt tattgcaact
     6481 tcctttccgg cacaaacggg accgccatcg tcaggaacaa ttcgtggcgt gaacctgtaa
     6541 acgaccttgt gtggtataat atcgtcattg gataatgttt cactaagatc ggcaggcatt
     6601 gtaaatgtcc ctccggcgga atatccggtt acttccgtct ccggttcgac gacaagatca
     6661 tacatccatt gaccacggac cgtggtattg ggattccgta ctgttatcag ggtgctttca
     6721 ccatggcaga caattgtgtc atttgaatga attccgataa ccggttcagg attcacccaa
     6781 atgcttgaac ttattgccgg aagcttgcat ttgccgtaaa ctgtgggagt aatctcataa
     6841 accacttcat gaacaactgc gccggtgttc tccagtgttt gcactatgga agaattcaat
     6901 ccgctattgt cgcccgaggc tcccgatatt tcagcaggga tatttaccgt ccatgcccag
     6961 gttgtcacag gcatattgtt ggtcatgacc agattgatgg tttcattgtt gcagatgaca
     7021 tctgttgccg gcgttacaac cgggttcggg accaccgcat agtggactgt gaccactgca
     7081 gtacatgtac ttgaattatc gtatatgtct gtaacagtca gtacaatatt gttgggacca
     7141 agatcctcgc agtcaaatgt gtttatgtca atactcaggc tggcgacagc agtgttgtca
     7201 tagcttcccc cgtcaacatc ctcgggatta attgatacca aaccggtatt aagatccaga
     7261 taaacatcaa tgttccggca aattgcggcc ggcggtacat tgtcggtgac tgtaacagcg
     7321 aagctgcagg atgcggttct tcctgaacca tctgtcacgg tccacgtaac atttgtcgtc
     7381 ccgacgggaa accgggcacc cgaaagactt gtagcattgt tgaagtcgtt aatcaggccc
     7441 gcaaccgcgc agttgtcatc tgctgacagc ggatcaaatt cggttccctg tactgtgtaa
     7501 tccggtccgt ttgtgtccgt aaacctgtcc cggggtccgg cacagcttat taccggatct
     7561 gttaaatcat tcacagtaac ggtgaagctg caggttgccg ataatcccgg agcgtcagtg
     7621 acagtatatg ttattgtagt tactccgcca ttaaatatgt aggatgacag gttattgatg
     7681 cctgatgaca gggaacggcc attggtggca cctgtcataa cccatgtgac agatgatatg
     7741 ttattgttcg gatccgagaa agtcacagca agtccgcctg ttagttcaac ctggcatgaa
     7801 gtcggcggag cattgactgt tatcggccgt agacatgaaa taaccggcgg agcattattc
     7861 agggtaacag tgacttcatt gcttgtttcc gatatcctgt tcatcagtgg atcaagcccg
     7921 gaaacggaag ccctgttgct aaagcttccc ctgttcacgt catccacagt tattgtatga
     7981 ttggcagtgc aggttacagt tcttcccgga gcgaggataa atggtgcgcc gggacatgat
     8041 aggatcgtat tcggatcact gacggttacc ccggtaaggg ttacatttcc ggtgttggtc
     8101 acaactatgg tataatgaat cacatcacct gcagtggtga agtatctctc cgccgtactt
     8161 ttcgatatgg cgacccccgg cctgatgtca gctctgacta tcgcataatc actttcagta
     8221 tagcggtttc cgttaaatgt gtaggaagct gtggtggtat taagaacctg accattattc
     8281 atgtcattct gggttatgat gtaagttgtt ggaaaggttc tgcttgtacc cgggattaaa
     8341 cttggaatgg tctgatcaag cccggtgacc ggatccgtta ctgaaatatc ggataatgtc
     8401 acattgccgt cattggtaac cataattgta taatccacct gatctccggc agctgaataa
     8461 cttgtctgca atgaactttt tgaaattgat aag
//
LOCUS       DAOOYG010000116         8472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9280_length_8472_cov_7.443388, whole genome shotgun sequence.
ACCESSION   DAOOYG010000116 DAOOYG010000000
VERSION     DAOOYG010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9280_length_8472_cov_7.443388"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PK288_09260"
     CDS             complement(<1..872)
                     /locus_tag="PK288_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19153.1"
                     /translation="MKRQIISLLFYSAFWLSFFIVARSYFLVSHCSEASGAGFTTMAG
                     TFTHGIRLDISATGYILILPLLASILFVWFSGKWFSAFMKWYTRIVLLAASVIIVVDS
                     VLYIYWGYRMDFTPFMYLKTPREAAASVSILKIFLMLLAILLLAGTAGIIYNKLFRSC
                     FSKPVNRSHRFAATLLFMLLSGSLIIPIRGGLGVAPIYAGSVYFSENMFANHASVNVV
                     WNVGSSFVNRKPASNPYVFADKDEAEEITRSLTISNGATEMVLTTARPNILIIILESF
                     GNALIGPLGGDPLTT"
     gene            complement(1083..1559)
                     /locus_tag="PK288_09265"
     CDS             complement(1083..1559)
                     /locus_tag="PK288_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HPM19154.1"
                     /translation="MCLKITNKTRIIFNFGVFKKRSNKFNKPVTMGKGDKKTRRGKII
                     RGTFGVRRRRKKTPKPELKLASPVVEKETTDKKLIKDKKKAPEVKEQAGIPEVTDAKT
                     VKDGIEPADSTPVKKSKPVKAAAEGKEKKAPREKKEAKIPKEVKEKKEAKPKKGEE"
     gene            1631..2098
                     /locus_tag="PK288_09270"
     CDS             1631..2098
                     /locus_tag="PK288_09270"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPM19155.1"
                     /translation="MRRKDREISDIAEIEEVIRKSDVCRIAIANEDLPYIVTMSFGYC
                     SGDRKRIYFHCAPEGKKLDMLDRNNFVCFEFDTDHILLKGPDACDYGMAFSSVVGWGR
                     ISRVSDNDERKEGMLAIMEHYSGKRDFSFRPEALAKTLILRLDITQMTGKRRH"
     gene            complement(2114..3103)
                     /locus_tag="PK288_09275"
     CDS             complement(2114..3103)
                     /locus_tag="PK288_09275"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPM19156.1"
                     /translation="MKIFLTGGSGFIGSRLTERLINEDHDVTLLLRDPGKDLWFDRNK
                     VRIVTGDIFDTGILAKEMKGCEVVFHLAAYTKPWSKDPELPYRVNVTGTINVLSAAIE
                     AGVKRVVMTSSCGTFGYSYNGSVVNELTVPENPLCTVYERTKAEAEQKALGFCSRGID
                     VIIVNPSRLYGPGVLTSGNSLTRIIRGYVRGTWRIIPGKGTAVGNYVFIDDVVDGHIK
                     AAIRGVTGEKYILGGENLSFSALFKIIGEESGRKRRMIRFPLAAIRDIIRLIRLGSRF
                     LGISPPITEEWIDKYMKDTVISSDKAVSVLGYKITPFRQGVRKTVEWLMTNHR"
     gene            3320..3640
                     /locus_tag="PK288_09280"
     CDS             3320..3640
                     /locus_tag="PK288_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPM19157.1"
                     /translation="MNIYVGSLHFKMNEAELREIFEEYGEVTSAKIIIDKYSGKSKGF
                     GFVEMPNEAEAKKAIEELNGAEVQGRKIIVNESIERADRRSNFRGGDNDRRGGVGNRR
                     DNYR"
     gene            3660..3860
                     /locus_tag="PK288_09285"
     CDS             3660..3860
                     /locus_tag="PK288_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPM19158.1"
                     /translation="MKGTVKWYDRVKGYGFLQTEDQKDIFVHKTGLEVSQADLETGQA
                     VEFEIEMKEKGPVAIKVRKIAQ"
     gene            complement(3984..5312)
                     /locus_tag="PK288_09290"
     CDS             complement(3984..5312)
                     /locus_tag="PK288_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM19159.1"
                     /translation="MMKDQKNLWTRRRFIGSAAAGITSAVLLPHLATSCKSKPVTEIK
                     LGFIGLGRQSMFLLSGFIQIPGVKVLAGCDVYGIKRKRFERRVNGFYANTGKAVRVET
                     YEKYEDLLARADIDAVVIAVPDHTHAMIAIAACNAGKDVYLEKPMTFTIREGQELKRV
                     VRDNDRILGVGSQQRSDPGFQHAVKLVQTGALGTISRVNAYVGAPPIPYDLPEEPLPE
                     DLNWDRWIGPLTAKIHFNNQLDPPISLDPEKDEEIWGAWRWYKEMGGGFTTDWGAHMF
                     DIAQWGLGMDKSGPVEISPIGDGTEFISWKYANGVIMTSEPFDDMLTKGVKFWGENGW
                     IEVARGYFNASDPKFNPPAAASSDDSGPYETRIPHQVNFIEAVKHHQDPVVPVEIGHS
                     SCTVCTLGNIACDLKRTIKWNPDTETFVDDTDGEATRRLHYEYRSPWKLV"
     gene            complement(5520..6236)
                     /locus_tag="PK288_09295"
     CDS             complement(5520..6236)
                     /locus_tag="PK288_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPM19160.1"
                     /translation="MTGPGRVPQYRRLYEILKRHIAEGIYREGDLLPSENELCQLYSM
                     TRPTVRQSLGKLAGEGYITKHQGKGSIVHQFPRDIGILSISGTTTAVGDRNLKTRIIV
                     RPVIKPWPDDFMFALSELEKESGCVYMERLRLLDDSPIFFDISYITNLNLPRITSRQF
                     ENRSLFQILREHYGVEVRGGEQRIRAIPASGRTGRFLGIARGQPVLHLERKMETSVNG
                     LFLYSSIYCNTIKYSIFGRF"
     gene            6438..7685
                     /locus_tag="PK288_09300"
     CDS             6438..7685
                     /locus_tag="PK288_09300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HPM19161.1"
                     /translation="MAIPVIMPRQGQSVETCIITKWFIKKGDRVKQGDILFSYETDKA
                     AFDFESPDEGTILEIFYPEGSEVPVLATVAVMGIPGESFAPFVPGAHPAQKPEPSGLP
                     GEATPGAVISGPAFQHPVHEQDHTKVRISPRARRLAADRGLNLAGISGSGPGGRIIFS
                     DIERAIADSRLKEPAPADETTEYTDQPLSNIRKLISKAMYDSLHNSAQLTHHMSADAR
                     KLLEARKRIKDDLAGDARRPDITLNDMICMCVIKALRSYPDINSHFLGDRIRTFNHVH
                     LGIAVDTQRGLIVPAVRNAGSMTIETLSEKLRAAADACKTGNIDPELIKSTSASFTVS
                     NLGNYGVEMFTPVLNLPQVAILGVCTVTYRPADTGNGIFGFVPFIGLSLTYDHRAVDG
                     GPATLFLREVKKQIEGFALLESQ"
     gene            7780..>8472
                     /locus_tag="PK288_09305"
     CDS             7780..>8472
                     /locus_tag="PK288_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPM19162.1"
                     /translation="MPKTHNIYPEEVRRPSFIEFGKIPVNQYDKSIEDERDNFSADDF
                     MRIYHDMVVIREFETMLNLIKTTNEYKGISYNHPGPAHLSIGQEAAAVGMAYNLDVDD
                     YIFGSHRSHGEIIAKGLSAIHKTDEDTLYSIMKNYFNGKTLAVVEKGFKGTVRELAKH
                     FLLYGALAEIFARENGFNKGLGGSMHAFFTPFGIYPNNAIVGGSGDISVGAALYKKIN
                     RKPGIVVCNIGDA"
BASE COUNT         2310 a         1972 c         1904 g         2286 t
ORIGIN      
        1 gttgtcaggg gatcgccgcc aagaggaccg atcagtgcat ttccaaagct ctcaagaatg
       61 atgatgagaa tattgggcct ggcagtggtt aaaaccattt cagttgcgcc attacttatt
      121 gtgagtgacc tggtaatctc ttctgcttca tccttatctg cgaaaacata tggattactg
      181 gcaggcttac ggttgacaaa ggagctgccg acattccata ccacatttac cgaagcgtga
      241 ttggcaaaca tgttttcgct gaaatacact gaaccggcat aaatcggggc caccccaaga
      301 ccgccgcgaa tcggtattat cagagaaccg gacaagagca tgaaaagcag tgtggccgca
      361 aacctatggc tgcggttgac aggtttgctg aaacagctcc tgaataattt gttgtatatt
      421 ataccggcag tacctgcaag caacagaatg gcgagcaaca tcaggaatat tttcaggatg
      481 cttacagaag cagccgcttc cctgggggtt ttaagataca taaatggtgt gaaatccatc
      541 ctgtatcccc agtaaatata gaggaccgaa tcaacaacaa tgattactga agcggccagc
      601 aaaacgatcc gggtatacca tttcatgaat gcactgaacc atttaccgct gaaccataca
      661 aacagtatcg atgccaatag gggaagaatc aggatatagc cagtggcaga aatgtcgagc
      721 ctgattccgt gggtaaaagt tccagccatt gtagtgaatc ctgcgccaga tgcctcactg
      781 caatgagaga ccaggaaata gcttcttgca acgatgaaga acgacagcca gaaggcactg
      841 taaaacaaca gggagataat ttgccttttc atttacatta aacctgtttc gcgctgcccc
      901 ataccctgtt caggctgcct gcacggagat aaattgccgt ttttgaaatt acccggcatc
      961 aaaaagatgt ttataaccta aaatatacct ggaaaacaga taatgccgga aaagctggat
     1021 ttgaaaactg gaacaatcac cacttctgat tgtgataaac ctgaaaagaa gaaccctttc
     1081 agtcattctt ctcctttttt cggttttgct tctttttttt cctttacttc ctttggaatt
     1141 ttagcttcct tcttttccct gggagctttt ttctcctttc cctcagctgc tgcctttaca
     1201 ggctttgatt tcttaaccgg tgttgaatca gccggttcaa ttccatcctt cacggttttt
     1261 gcatcagtca cttcagggat tccggcctgt tctttaacct caggtgcttt ctttttatcc
     1321 ttaataagct tcttgtctgt ggtctccttc tcaacaacag gagaagcaag ctttagttca
     1381 ggctttgggg ttttctttct ccgccgcctt actccgaaag tgccacgaat tatcttacct
     1441 ctcctggttt ttttgtcacc ttttcccatt gttacaggtt tattgaattt attactccgt
     1501 tttttgaaga ctccaaagtt aaaaataatc ctcgttttat tggtaatttt aaggcacaat
     1561 aaacaaaatt agcaggaatc aggtcagaca ggatgccgta ggattccggt aaattttaaa
     1621 aagttctgaa atgagaagaa aggacaggga aatcagcgac atcgcggaaa ttgaggaagt
     1681 gatccggaaa tcggatgtat gccgtattgc aattgccaat gaagatctgc cttacattgt
     1741 gacgatgagt ttcgggtatt gcagcgggga caggaagagg atttattttc attgtgcccc
     1801 ggaaggaaaa aagctggata tgcttgacag gaacaacttt gtttgttttg agttcgatac
     1861 cgatcacata ctgttaaaag gccctgacgc ctgtgattac ggaatggcat tcagttcagt
     1921 tgtgggatgg ggaaggatat caagggtgtc cgacaatgat gagaggaagg aaggaatgct
     1981 tgcaataatg gagcattatt ccgggaagag ggatttttca ttcagacctg aggctttggc
     2041 caaaacactg atcctgcgtc tcgacataac tcaaatgaca ggtaaacgcc gccattgaaa
     2101 atggatgaaa attttacctg tgatttgtca ttagccattc aacagtcttt ctaacgccct
     2161 gtctgaaagg tgtgatctta taacccagaa cggaaacagc cttatcgctg gaaatgacgg
     2221 tatctttcat gtatttatcg atccattctt cagtaattgg aggagatatt ccaaggaacc
     2281 gggaacccag gcgaatgagt cttatgatgt ccctgattgc agccagcggg aagcggatca
     2341 ttcgcctttt tctccctgat tcttccccga taatcttaaa aagggctgag aatgagagat
     2401 tttcacctcc caggatatat ttttctcctg taactcccct gatggctgct ttaatatgac
     2461 catcaactac gtcatcgatg aaaacatagt tacccacagc ggttcctttc ccgggtatga
     2521 ttctccatgt acccctcaca tatcccctga tgattcgcgt caatgagtta ccgcttgtca
     2581 ggacacccgg gccgtacagg cgggacggat tgacaattat tacatcgata ccccggctgc
     2641 agaatccgag agccttttgc tctgcttcag cctttgtcct ttcatatact gtacaaagag
     2701 gattctcggg tactgttaat tcattaacaa ctgacccgtt ataagagtat ccgaaagtac
     2761 cgcaggaaga ggtcatgaca acccgtttca cccctgcctc aattgccgca ctgaggacat
     2821 tgatggtccc ggtgacattg accctgtagg gcagctcagg atcctttgac caaggctttg
     2881 tgtaggcagc cagatgaaag accacctcac aacccttcat ttcctttgca aggatgccgg
     2941 tatcaaaaat atcccctgta acgatcctca ccttgttcct gtcgaaccag agatccttgc
     3001 ccggatccct cagcagcagg gtaacatcat gatcttcatt tatcagcctt tctgtcagcc
     3061 tgctaccgat aaagccgctg cccccggtca gaaatatctt catgcgagca atgtttattt
     3121 tctcaagaac aattttaagt attatctgtt agtaaataaa aaaatacagt gcgaaaacaa
     3181 tcccggtttc gaatggcttt caatcaggtt ggcaggtagc aaaaaggtat taaaatacaa
     3241 catattatga agttttattt tttgttctca aaaataaatc tgacctttgc tgatgaatat
     3301 ttattttaat ttttgaatta tgaacattta tgtaggaagt cttcatttta aaatgaatga
     3361 agcagagcta agagaaatct ttgaagagta cggtgaagta acatccgcga aaattattat
     3421 tgacaagtac tcaggcaaga gtaaaggatt cggtttcgtt gagatgccta acgaggctga
     3481 agcaaagaag gccattgaag aactcaacgg tgctgaagtc cagggcagaa aaattattgt
     3541 aaacgaatca attgaaagag ctgacagaag aagtaatttc agggggggag acaacgatcg
     3601 tcgcggtggc gttggcaaca gaagggataa ttacagatag aaaataaaga ggacgaacta
     3661 tgaaaggcac tgtaaagtgg tatgacaggg taaaaggata tggttttctt cagacggaag
     3721 accagaagga tattttcgtt cacaagacgg ggctggaggt atctcaggcc gatctggaaa
     3781 ccggacaggc cgtggagttt gagattgaaa tgaaggaaaa gggccctgta gccattaagg
     3841 tcaggaaaat tgcacaatag ctttagtgga taccataatg cagaaaagag gctgtatatc
     3901 ctgcagcctc ttttttttat ttgccggcag tccaaaatcc tggcagccgt cagaaagaag
     3961 tattcacata tcccctgtct ttttcagaca agcttccatg ggctgcggta ttcataatgg
     4021 aggcgcctgg tagcttcccc gtcagtgtcg tctacgaatg tttctgtatc agggttccat
     4081 ttgatggtcc tcttcaggtc gcaggcgatg ttacccaggg tacataccgt gcagctgctg
     4141 tgtccgatct caaccggaac cactggatcc tggtgatgtt tcactgcttc gataaaattc
     4201 acctgatggg gtatccttgt ttcatacggg ccgctgtcgt ccgatgatgc ggctgcaggc
     4261 ggattgaatt taggatcgga ggcattgaaa tagccgcgtg ctacttcgat ccacccgttt
     4321 tcgccccaga acttgactcc ttttgtaagc atgtcatcaa acggttcgga ggtcattatc
     4381 accccattgg catatttcca ggaaatgaat tcggtcccgt cgccaatcgg tgatatttca
     4441 acggggccgc tcttgtccat acccagtccc cactgggcta tgtcgaacat gtgtgctccc
     4501 cagtcagtag taaagccgcc gcccatctcc ttgtaccagc gccaagcgcc ccatatctcc
     4561 tcatcctttt caggatcaag cgagatgggt ggatccagct gattgttgaa atggattttt
     4621 gccgtcaggg gccctatcca cctgtcccag ttaagatctt ccggcagcgg ctcctctggc
     4681 agatcatatg gtatgggagg tgccccaacg taagcattca ccctggaaat cgttccaagt
     4741 gcccctgtct gaacaagctt cacagcatgc tggaaccccg gatccgaacg ctgctggctc
     4801 ccgaccccga gaatcctgtc attgtcgcgg accacccttt tcagctcctg cccttccctg
     4861 atggtaaaag tcatcggctt ctcaagatac acgtccttgc cggcattgca ggcagcaatg
     4921 gcaatcatgg catgagtatg gtcgggaaca gctattacaa ccgcatcaat atcggcacgg
     4981 gcaagcagat cctcgtattt ctcataagtt tcgaccctga ctgccttacc ggtatttgcg
     5041 tagaacccat tcaccctcct ttcaaaacgc tttcttttaa tgccgtatac gtcacaaccg
     5101 gccagaacct tcacgccggg gatctggatg aagccactga gcaggaacat cgactggcgg
     5161 cccagtccga tgaaacccag tttaatttca gtaactggct tgctcttgca cgatgtcgcc
     5221 aggtgtggca gcagtacagc acttgttatt cctgcggcag ccgagccaat gaaccggcgc
     5281 ctggtccata aattcttctg atctttcatc ataatacaat tttgatttta atggttcaat
     5341 ggaaccggca tattctaaat cagacctgtt catatctgtt tatcgatcat gcgggtttca
     5401 taggtaaatg tttgttttta ggtcaaaata tccgatatgt ctaaaaatcc aaaattcaca
     5461 tggcaacttt attggcccct taaaattaat cctttattaa aatctttaaa agattttttt
     5521 cagaatcttc caaatattga gtatttaatg gtattgcaat aaattgatga ataaaggaac
     5581 agcccgttca cgctggtttc catttttctc tcaagatgca ggaccggctg cccccttgca
     5641 attccgagaa agcgaccagt ccggccactg gcagggatgg ccctgatccg ctgctctcct
     5701 ccccttacct caacaccgta atgttccctc aggatctgga aaagtgaccg gttttcaaac
     5761 tgtcgcgacg ttattctcgg aagattcagg ttggtaatat aactgatatc aaaaaaaatt
     5821 ggcgaatcat ccagcagacg gagtctttcc atgtaaacgc atcccgattc cttttccagt
     5881 tccgagaggg caaacatgaa atcatccggc cacggcttga tcactggcct gactatgatc
     5941 ctggttttaa gattcctgtc gccgacggca gtggttgtac ccgatatgga aaggataccg
     6001 atatccctgg gaaactggtg tacaatgctt cccttcccct gatgcttggt tatataaccc
     6061 tcccccgcca gttttcccag cgactggcgg accgtcggcc ttgtcattga ataaagctga
     6121 cacagctcat tctccgatgg aagcaggtca ccctccctgt aaataccttc ggctatatgc
     6181 ctcttcagta tttcgtaaag tcttctgtat tgcggaaccc ttcccggtcc tgtcataaaa
     6241 atcatttatt tcgtaaatat ataaaaaact acttgtaaat acaagtaata ttttttattt
     6301 ttgtaagcac tggtaattaa ctagattgaa aggcacaata tttataaagc ctcacttatt
     6361 attacaagtt attaaatctt tacatagagc taccggaaca tttaacgacg ttttttcaaa
     6421 attcaaccag gcaaaacatg gcaataccag taataatgcc caggcaggga caatcggtgg
     6481 aaacctgcat tataaccaaa tggttcatta aaaagggtga ccgggtgaaa cagggggata
     6541 ttcttttttc gtatgagacc gacaaggccg cattcgattt tgagtcgcct gacgaaggta
     6601 ctatcctcga aattttctat ccggagggat cagaggtgcc cgtccttgct actgtggcag
     6661 ttatgggaat ccccggagaa tccttcgctc cattcgttcc gggagcccat ccggcacaga
     6721 agccggaacc atccggactg cctggagagg ccacacccgg tgcagttatt tccgggcccg
     6781 ctttccagca tcccgtccat gaacaggatc ataccaaggt tagaatttca ccccgtgcca
     6841 gacgcctggc tgctgacagg ggacttaacc ttgcgggcat atccggatca ggaccaggag
     6901 gaaggattat tttcagcgat atagagagag ccattgcgga tagccgtcta aaggaaccag
     6961 ccccggctga tgaaacaact gaatacaccg accagccact ttcaaacata cggaagctga
     7021 tctcaaaggc aatgtacgat tctcttcata attcggccca gcttacccac cacatgagtg
     7081 ccgacgccag aaaacttctg gaagcccgga aaagaataaa ggatgacctg gcaggcgatg
     7141 ccagaagacc cgacattacc ctgaatgaca tgatttgcat gtgtgtgata aaggctctgc
     7201 gaagttatcc tgatataaac agtcatttcc tcggcgacag gatcaggacc ttcaaccatg
     7261 ttcacctggg aattgccgtt gatactcaga ggggattgat tgttcccgcg gtgaggaatg
     7321 ccggctcaat gaccattgaa acactttcgg aaaagctcag ggcggctgcc gatgcctgta
     7381 aaacgggaaa cattgatccc gagctgataa agagcacctc ggcatccttc acggtgtcaa
     7441 atctcggcaa ctacggagtt gaaatgttca ccccggtgct gaatcttcca caggtggcaa
     7501 ttctgggcgt atgtacggta acataccggc ctgccgatac cggcaatggt attttcgggt
     7561 tcgtcccttt catcggactt tcacttacct atgaccaccg ggctgtcgac ggcggaccgg
     7621 caacgttgtt cctgcgcgaa gtgaaaaaac agatagaagg attcgcttta cttgagtctc
     7681 agtaatggtt aatccggaat cttcaacatt aaacattaaa tatcttatac caggtatttt
     7741 acatttaaca acccgcaatg aactattaac tggaaaaaca tgcctaaaac tcataacatt
     7801 taccctgaag aagtccgcag gccttcattc attgaattcg gaaagatccc ggtaaaccag
     7861 tatgacaaat ccatcgaaga cgaaagggat aatttctccg ccgacgattt catgaggata
     7921 tatcacgata tggttgtgat acgggaattt gaaacgatgc tgaatctgat caaaacgaca
     7981 aatgaataca agggcatttc ctataatcat ccggggcctg cgcatctttc gatagggcag
     8041 gaagctgctg cggtgggaat ggcatacaat cttgatgttg atgactatat tttcggatcg
     8101 caccgcagtc acggagaaat tattgcaaag gggctttctg ccattcacaa gactgacgag
     8161 gacacccttt attcaataat gaagaattat tttaacggca aaaccctggc agttgttgag
     8221 aaaggcttta agggtacagt cagggaactt gcaaagcact ttcttcttta tggtgccctc
     8281 gccgagattt ttgcgcgtga gaatggcttt aacaagggtc ttggaggatc aatgcatgct
     8341 tttttcactc cttttggcat ttatcccaat aatgccattg tgggcggttc gggcgatatt
     8401 tcggttggag cggctctata caagaagatc aacaggaagc ccgggatagt ggtatgcaac
     8461 attggcgatg ct
//
LOCUS       DAOOYG010000117         8278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9572_length_8278_cov_7.630670, whole genome shotgun sequence.
ACCESSION   DAOOYG010000117 DAOOYG010000000
VERSION     DAOOYG010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9572_length_8278_cov_7.630670"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PK288_09310"
     CDS             <1..367
                     /locus_tag="PK288_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19163.1"
                     /translation="LHENYDVSIYPSAAEAFVGKFWEVDDNVLSLAERISSAPPSSGP
                     ELVHIAHEEGCKDLFPSISGEEKSYLASDGKIPSYSRWAGKIKDQLIAPDTLLTLAGL
                     ESFKSDQKKLDERILGIIG"
     gene            complement(368..1108)
                     /gene="rlmB"
                     /locus_tag="PK288_09315"
     CDS             complement(368..1108)
                     /gene="rlmB"
                     /locus_tag="PK288_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007748020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPM19164.1"
                     /translation="MAGETDYIFGIRAVIEAVRSGRQLDRLLIRHGLQGSLYHELITE
                     IRNRNIPYQIVPQERIDLVTKKNHQGVLAWLSLIEYQSVSDILPLIYEKGEDPLVIVL
                     DGISDVRNFGAIIRTAECMGVHAAIVPERGSARITADAVKTSAGALNFFPVCREKSIL
                     KSIGFLKDSGLRIFAATEKRGTDLSRACLTGPSALILGSEERGISRELLSVSDMEIRI
                     PMEGRIGSLNVSVAAGIFLHEIKRQRSI"
     gene            complement(1114..2457)
                     /locus_tag="PK288_09320"
     CDS             complement(1114..2457)
                     /locus_tag="PK288_09320"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPM19165.1"
                     /translation="MKRISSFRGGLPSLAALLLAAGILVSCGSTQKAVIDSKDLSYLY
                     NPTKSPIKPRYNIVNHGDENSVLSVKFFAAELFFSEANPQGVPMAQLVISARLHNVTA
                     GGILADTAYSSLNIVKKESVPEYVFRISFRVEPGNEYMAELKILDKLRMQIVQAFIPF
                     NTLSKYNRYNFTALGYFLKNELFNPVVRENEYVDLIYNGGRTDSLYISFYKPLKEIPY
                     PPSMLLPEKTVDYDPERVIAVPYSDTLPMMFRTEGIYFCSADRQINEGFTFFNFGNTY
                     PNMTHAEVMIEPLAYLASEDEMNRMRNAPGPKAALDEFWIGCGGNVEKARELLRIYYT
                     RILYSNYYFTSYKEGWRTERGMIYTIYGPPDKVYKTADGERWGYRKPVLRSSWGGRYH
                     LREEYLFFNFRYRANRLTDNDYYLIRSETLVTYWDRAVASWRKGIVFRLDNPDDI"
     gene            complement(2454..3257)
                     /locus_tag="PK288_09325"
     CDS             complement(2454..3257)
                     /locus_tag="PK288_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPM19166.1"
                     /translation="MNSSKAAAMALSKLEKEYHVHVYEAGPDGKLNLYSLFDYFQDIA
                     SEHAIKLGYGRDDLLEQNNFWVLSRIYAEISEMPEWGEKITVTTWPRGIDRLFALRDY
                     EVRNQQGRRVSIATSSWLIIDRITKKIKRPDDTLSSFKAQAEFRNPLPRNAGKLDAAG
                     KDSKITSLFRVRVSDLDVNLHTNNVNYLKWVIDTHSQEFILDNIPVFVEINYLAESRF
                     DDEIIIRMSEKDGEVKLTDYSIFRTTDDTELSRVRLMWNKAVNRKTEKK"
     gene            complement(3214..5172)
                     /locus_tag="PK288_09330"
     CDS             complement(3214..5172)
                     /locus_tag="PK288_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPM19167.1"
                     /translation="MVSVQDISVSFGSYDLLTNISFLINDQDRIGLTGKNGAGKSTLL
                     KIIAGLQSPTSGIVDMSRNVTIGFLPQQMSVSDSTTVFNETVSAFSDILSLSAVIESC
                     TAEIARRTDYESAEYLRLCDRLAETEERFRLLGGANYLAETEQTLLGLGFEREEFSRP
                     TSELSGGWRMRIELAKLILKKPSLFLLDEPTNHLDIESIQWLESYLDSYPGAVMMVSH
                     DKAFLDNITKRTIEISLGRIYDYRASWSKYIVLRTERREQQVAAYRNQQKMIEDTEKF
                     IERFRYKATKAIQVQSKIKQLEKIERIEVDEEDTSAINFSFPPAPRSGTVVVRAENLS
                     KDFGRVKVLRNVAITITRGEKVAFVGRNGEGKTTFSRIITGELDYEGTLKIGHNVRTG
                     YFAQNQDELMDDDKTVFETVDAAARGEIRSRLRDILGAFLFRGDDIEKKVRVLSGGES
                     SRLALVKLLLEPYNLLVLDEPTNHLDMRSKDILKKALMKYDGTLIVVSHDRDFLDGLV
                     AKVFEFRHNRIKENIGGIYDFLSKKKIENLRDIEAKDRSREKNTNRELTSAKQQYLER
                     KEYEKRLRKLRRKLEESEHKIEKMERELSEMDIRLMSSSIPDESADIYRDYENLKLRL
                     NKEMESWALYSHEVDEFIKSSSNGFEQT"
     gene            5293..6552
                     /locus_tag="PK288_09335"
     CDS             5293..6552
                     /locus_tag="PK288_09335"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPM19168.1"
                     /translation="MKNFNKNRLKRKFLAIISVFLFLTGSCTREEIHYYDFYVSKEFK
                     TSYTKQKIVSLLNLLPDEIPEIIALKQSVNYDVIIYKIVYNTQISGKTIRASGLLCLP
                     GSEGEFPVVCFQNGTNTVNLWSPSESPNNPVIQIIEAVASMGYVVLIPDYPGFGESAG
                     IPHPYLIAAPTIRSIIDMLFGAGEAIENEFPGISLKNEYYLLGYSQGGWATLNLHKSL
                     EQDYSSKINLAGTVCGAGPYDLKKSFSDILASQTYGMPVYLGYIVYAYKAYGLVKNTM
                     QEIFNEPYSSRIGTLFDGSHTSAQINEKLTFSIPELVNENLIEGFETSPDYSSLRQAL
                     EENSIQPWKTKIPLLFIHGGDDTSVEPLLTEMIYEKMILQGTSPEICKKEIIPGLDHG
                     AAYIPFLLKGFTFLRNVKDNKISHWEN"
     gene            6571..7977
                     /gene="rlmD"
                     /locus_tag="PK288_09340"
     CDS             6571..7977
                     /gene="rlmD"
                     /locus_tag="PK288_09340"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPM19169.1"
                     /translation="MGRKKEYPIFGNVRITDIGAEGNALARVNNMVVFVPMLIPGDVV
                     DIQVVRKRKNYLEGRVLRFREYSPDRVEPVCIHFGICGGCRWQHLPYELQLHYKQKQV
                     ADNLERIGKINVTDISRIAGSADQFRYRNKLEFTFSDKRWLTREEIASDNEFAGEDAI
                     GFHIPGLFDKVLDIRECHLQPEPSNLIRNSVREYAHRDRMTFFNKKEQTGFLRNMIVR
                     NNQEGDVMVIMVFFYEDPERRKGLLDHLVAAFPEISSLFYVVNPKKNDSLNGLKPVLY
                     KGEDHLAERMEGLKFRIGPISFYQTNSRQALQLYKIARDFAGLRGTETVYDLYTGTGT
                     IACFVAGQSGRVIGIDNVGEAVQDARYNAANNNLGNVSFFMGDIKSLLTEQFFAENGR
                     PDIVITDPPRAGMDKKVINAIIDAEPEKIVYISCNPSTQARDIQIMNMKYQVVRIQPV
                     DMFPQTHHVENVVLLRKV"
BASE COUNT         2228 a         1869 c         1851 g         2330 t
ORIGIN      
        1 attgcatgag aattatgacg tgagcatata tccatccgct gcggaagctt tcgtcggaaa
       61 attctgggaa gttgatgata atgtcctttc gcttgctgaa aggatatcgt ccgcgccgcc
      121 ttcatcagga cctgaactgg tgcatattgc acatgaggaa ggatgcaagg atctttttcc
      181 ttcaatttcc ggggaagaga aatcatacct ggcttccgac ggcaaaatcc cttcctattc
      241 aaggtgggcc ggcaaaatta aggaccagct cattgcccct gatacccttc ttacactagc
      301 agggctggaa tcattcaaat cggatcagaa aaagcttgat gagaggattt tggggataat
      361 tggctgatca gatactcctt tgccttttta tttcatgcag aaaaataccg gctgcaaccg
      421 atacattgag ggatcctatt cttccttcca tcgggatcct gatttccatg tcagacaccg
      481 acaataattc cctgctgata cccctttctt ccgagccgag tataagggct gatggcccgg
      541 tcaggcaggc ccgcgaaaga tcggtccccc gtttttcggt agctgcaaag atcctgaggc
      601 ccgaatcttt aagaaacccg attgatttca gaatgctctt ttccctgcat acggggaaaa
      661 agttaagtgc acctgccgaa gttttgacag catctgcagt gatccgggca gaacctcttt
      721 ccggtacaat ggcagcatga actcccatgc attcggctgt tctgattatt gcgccgaagt
      781 tcctaacgtc agagataccg tcgagaacaa tgacaagagg atcttccccc ttttcataaa
      841 tgagtggaag aatatctgaa acagactgat attcgatcag ggaaagccag gccagcactc
      901 cctggtgatt tttttttgtt accaggtcaa ttctttcctg tggcactatc tggtatggga
      961 tgttcctgtt ccttatttca gtaatgagct catgataaag ggatccctga agtccatgcc
     1021 ttatcagaag ccgatcgagc tgccttcctg atctgacagc ttctattact gccctgatgc
     1081 caaaaatata gtcagtttca ccggccatcg cagttaaata tcatcagggt tgtcgaggcg
     1141 gaaaacaatg cccttccgcc agctggcaac agccctgtcc cagtaggtta caagtgtttc
     1201 actgcggatg aggtaataat cattgtcggt taaccggttt gccctgtacc tgaagttgaa
     1261 aaacaggtat tcttccctga gatgatatct tcctccccat gatgatctta gtaccggttt
     1321 acgataaccc caccgttcgc catccgctgt tttgtatact ttgtcgggcg gcccgtaaat
     1381 agtataaatc attccccttt cagttctcca tccttcctta tatgaggtga agtaataatt
     1441 tgaatagaga atccttgtgt aatagattct aagaagctcc ctggcttttt cgacattgcc
     1501 gccgcagccg atccagaatt catcgagggc tgccttggga ccaggggcat tcctcatcct
     1561 gttcatctca tcttcggatg caagataagc caggggttct atcataacct cggcatgtgt
     1621 catattcgga taggtattgc cgaaattgaa gaacgtaaaa ccttcgttta tctgcctgtc
     1681 ggctgaacaa aaatatatgc cttcagttct gaacatcatc ggaagagtgt cagaataggg
     1741 aacggcaatc accctttcgg gatcgtaatc gacagttttt tcaggcagca gcattgatgg
     1801 gggatatggg atctccttga gaggcttgta aaaggaaata taaaggctgt cggttctacc
     1861 cccgttatag ataagatcaa catactcgtt ctccctcaca accggattga acagctcgtt
     1921 tttcagaaaa taacccaggg cagtgaagtt gtacctgttg tattttgaaa gtgtattgaa
     1981 tggtataaac gcctggacta tctgcattct caacttgtca aggatcttta attcagccat
     2041 gtattcattc ccgggttcaa ccctgaatga tattctgaaa acatactcag gcactgattc
     2101 ctttttgaca atattcagac tgctgtaagc agtatcagca aggattccgc ccgcagtcac
     2161 attatgcagc cgggctgata ttacaagctg ggccatggga acaccttgcg gattggcttc
     2221 ggaaaagaaa agctcagccg caaaaaactt cactgataat actgagtttt catcgccatg
     2281 gttgacaata ttatagcggg gtttaatcgg acttttggta ggattgtaca ggtatgacag
     2341 atccttggaa tcaataaccg ccttttgggt ggatccgcat gataccagta tcccggcagc
     2401 aagaagcaat gctgccaggg atggtaaacc accacggaat gaactgatcc gcttcatttt
     2461 ttttctgttt tccggttgac tgctttattc cacatcaatc tgaccctgga caattcagta
     2521 tcatctgtgg tcctgaaaat cgaataatca gtaagtttaa cttctccgtc cttttctgac
     2581 attcttatga taatttcatc atcaaagcgg gattcggcaa gatagtttat ttcaacgaat
     2641 acagggatat tatccagaat aaactcctgg ctgtgagtgt cgataaccca tttcaggtaa
     2701 tttacgttat tcgtatgaag attaacatcc agatcgctga cccgcaccct gaaaagtgat
     2761 gttattttcg aatccttacc agcggcatcc agttttcctg cattcctggg cagcggattc
     2821 cggaattctg cctgagcctt gaaagaactc agggtatcat caggtctttt tatctttttt
     2881 gtaatcctgt cgattatgag ccacgaagaa gtagcaatgg atacccgcct gccctgctgg
     2941 ttccttacct cataatctct cagtgcaaat aaccggtcga tgccgcgggg ccatgttgtg
     3001 acggttattt tttctcccca ttccggcatt tccgatattt cggcatagat cctggagaga
     3061 acccagaaat tattctgctc gagcaggtcg tcccttccgt atccgagctt tattgcatgt
     3121 tcggaagcaa tgtcctggaa atagtcaaac agtgaatata aattcaattt tccatcaggt
     3181 cctgcttcat aaacatgaac atgatactct ttttcaagtt tgctcaaagc cattgctgct
     3241 gcttttgatg aattcatcaa cttcatggga ataaagcgcc cagctttcca tttccttatt
     3301 cagcctgagc ttaaggtttt cgtagtccct gtagatgtcc gcactctcgt caggaataga
     3361 ggagctcatc agcctaatat ccatttctga aagttccctc tccatttttt caattttatg
     3421 ttctgattcc tcgagtttac gcctgagttt tctgagcctt ttttcatatt cctttctttc
     3481 aaggtattgc tgtttggcgg acgtcaattc ccggttggta tttttttccc gggacctgtc
     3541 ctttgcctca atatctctca gattctctat ttttttctta ctgaggaagt catatatgcc
     3601 gccaatattt tccttgatcc tgttatgcct gaattcaaag acctttgcaa caagaccgtc
     3661 gagaaaatcc ctgtcgtggg aaacaacaat taacgtgcca tcatatttca tcagggcctt
     3721 tttcaatata tctttcgacc gcatatcgag atggtttgtc ggctcatcaa ggacaagcag
     3781 attgtatggt tcaagcagca gcttcacaag cgccagccgg gagctctcgc cgcctgacag
     3841 caccctgact ttcttttcaa tgtcatcacc cctgaataga aatgcaccaa gaatatcccg
     3901 gagccgtgat cgtatttctc ccctggctgc ggcatcaacc gtttcaaaaa cggttttgtc
     3961 atcgtccatc agctcatcct ggttctgggc aaaatacccc gttctgacat tgtggcctat
     4021 tttcagggtc ccttcataat caagctcgcc ggtgattatt cgtgaaaagg tggtctttcc
     4081 ctcaccattc cggccgacaa aggcaacttt ttcgcctctg gttattgtta ttgccacatt
     4141 gcgaaggact ttcacacggc cgaaatcctt cgacaggttc tcagccctga caactaccgt
     4201 accggaacgg ggagccgggg gaaaactaaa gtttatcgca cttgtgtctt cttcatcaac
     4261 ttcaatccgt tcaatctttt cgagctgctt gattttggac tgtacctgga ttgcctttgt
     4321 agccttgtat ctgaatcttt caatgaattt ctccgtatcc tcaatcattt tctgctgatt
     4381 cctgtaagcc gcaacctgct gctccctcct ctcagtcctc agaacaatat actttgacca
     4441 cgaggccctg taatcatata tccggccaag ggaaatctca atggtccttt ttgttatgtt
     4501 atccaggaaa gccttgtcgt gtgaaaccat cataacagca ccgggataac tgtcgagata
     4561 tgattcaagc cactgtatcg attcaatatc aagatggttc gtcggctcat cgagaaggaa
     4621 cagggatggt tttttcagga tcagcttggc aagctcaatt cgcatcctcc aaccgccgct
     4681 taattcactt gttggacggc tgaattcttc cctctcaaaa cccagtccga gcagagtctg
     4741 ttccgtttcg gcaaggtaat ttgccccgcc cagaagcctg aacctttcct ccgtttccgc
     4801 caggcggtcg cataatctca gatattcagc cgattcataa tctgtcctcc tggcaatctc
     4861 cgctgtacag gactcaatta ccgccgacag cgaaagaata tccgaaaagg ctgataccgt
     4921 ttcattgaag acggtggtgg aatcactgac agacatctgt tggggaagaa aaccaatggt
     4981 cacattcctc gacatgtcta caatcccgga tgtcggactc tgaagccctg caataatctt
     5041 caggagagtg gattttcccg ccccgttttt gccggttagt ccgatgcggt cctgatcatt
     5101 tatcagaaaa gatatgttgg tcagcagatc ataacttccg aaagagactg aaatatcctg
     5161 gacagaaacc atatattcat atttcgccgc aaagatagaa aaagtctgaa agcttccgaa
     5221 gcggtggttt aaatgtgaag agttatattt gcaggtacgg tttttgacag atcctggtag
     5281 ttattagaac agatgaaaaa ctttaataaa aacagactga aaaggaaatt cctggcaatc
     5341 atttctgtct ttttgtttct taccggctca tgcacaaggg aagaaataca ttattatgat
     5401 ttttatgttt caaaggagtt caagacctca tatacaaagc agaaaattgt cagtctgctt
     5461 aatcttctgc ctgatgaaat ccctgaaata attgccctta aacagtcggt taactacgat
     5521 gtgattattt ataaaatcgt atacaatacg caaatttcag gaaaaacgat aagagcttca
     5581 ggccttttgt gtcttccggg cagtgaagga gaattcccgg tagtttgttt ccagaacggg
     5641 accaatacag ttaacctgtg gtccccgagt gaaagtccga acaatccggt aattcagatc
     5701 attgaagcag tcgcgagcat gggatatgtg gtgttaattc cggattatcc cggattcggg
     5761 gaatcggccg gaatacccca tccatatctt atagccgcac ctacgatccg gtcaattata
     5821 gatatgcttt ttggcgcggg cgaagccatt gaaaatgaat tcccgggtat cagcctgaaa
     5881 aacgaatatt accttttggg ttattcgcag ggaggatggg caacccttaa tctgcacaaa
     5941 tccctggaac aggattacag tagcaaaatt aatctggcag gtaccgtgtg cggcgccggg
     6001 ccatacgatc tgaagaagtc tttcagcgat atactggcat cccagaccta cggaatgccg
     6061 gtatacctgg gatacatcgt ttatgcatat aaagcctacg gcctggttaa gaacacaatg
     6121 caggagatat tcaacgaacc atattcttca aggattggaa ccctatttga tggcagccat
     6181 acttcagccc agataaatga aaagctgaca ttttcaatac ctgagcttgt aaatgaaaac
     6241 cttattgaag gattcgaaac atcgcccgat tattcttcac tgcggcaagc acttgaagaa
     6301 aacagtattc agccctggaa aacgaagatc ccgttattat ttattcacgg aggagacgat
     6361 acaagtgtag agccgttgtt gacagagatg atttatgaaa aaatgatcct gcagggaaca
     6421 tcaccagaga tctgcaaaaa ggaaataatc cctggtctgg atcacggtgc cgcttacatt
     6481 ccgttcctgc tgaaaggatt cacattcctg cgaaatgtga aggataataa gatttctcat
     6541 tgggaaaatt gatctctaca aaacagagac gtgggcagaa aaaaggaata cccaatattt
     6601 ggaaatgtaa ggattacgga tatcggagcc gaaggaaacg cattggcaag ggtgaataat
     6661 atggttgtgt ttgtgccgat gctgatcccg ggcgacgttg tcgatattca ggttgttaga
     6721 aagcgaaaga attatctcga aggcagggtg ctgagattca gggaatattc gcccgacagg
     6781 gtggagccgg tttgcattca tttcggcatt tgcgggggct gcagatggca gcaccttccc
     6841 tatgagcttc agctgcatta taaacagaag caggtggctg ataaccttga acggatcgga
     6901 aaaataaatg tgactgatat cagccggata gcaggttctg ctgatcagtt cagatacagg
     6961 aacaagctgg aatttacctt ttcagataaa aggtggctca cccgggagga aatagcatca
     7021 gacaatgaat ttgccggaga ggatgcaatt gggtttcata ttccaggatt gtttgacaaa
     7081 gtgcttgaca tcagggaatg ccatttacag ccggaaccct caaatctgat aaggaattca
     7141 gtgagggagt atgcacacag ggacaggatg acctttttca acaagaagga acaaacagga
     7201 tttctcagaa atatgattgt ccggaacaac caggaggggg acgtaatggt aataatggta
     7261 ttcttctatg aggatccgga aagaaggaaa gggctgcttg atcaccttgt tgcggcattc
     7321 cctgaaatct cctccctgtt ttatgttgtc aatcccaaga agaatgattc ccttaacggt
     7381 ctgaagcctg tattatacaa aggtgaggat caccttgcgg aacgaatgga aggtttgaag
     7441 ttcagaatcg gaccgatatc cttttatcag acaaattcaa ggcaggcttt gcaactttat
     7501 aaaattgcaa gggattttgc tggactccgg ggaacggaaa ccgtatacga tctttacacg
     7561 ggcaccggaa ctattgcatg ctttgttgcc ggacaatccg ggagggtaat tggtattgac
     7621 aatgttgggg aggctgttca ggacgcccga tataatgcgg cgaataacaa ccttggtaat
     7681 gtcagctttt ttatgggtga tatcaaatct ttgctgacag aacagttttt tgctgaaaac
     7741 ggccggcctg acattgtgat tactgatccc cccagggcag gcatggataa aaaggttatt
     7801 aatgcaataa tcgatgcgga gccggaaaag atagtgtaca taagttgcaa tccttcaacc
     7861 caggctaggg atattcagat catgaacatg aaataccagg ttgtccgcat tcaacctgtg
     7921 gatatgtttc cccagaccca tcatgtcgaa aatgtggtgc tcctcaggaa ggtataattg
     7981 ttgggttatt attttgcact ttattactga tttcctattt ttgtcgcgcc cggtttagta
     8041 taattccggg aggcggctgg ctttccataa cagaagccgg tacgtaggaa gataattgct
     8101 taaaccagtc ttgggaattt tggactaaac tgccgggcac cggagactat agggagagat
     8161 ggatgagtgg cttaaaccag tcccgattgc tcgggactaa actgccagcc atcagaaaaa
     8221 aaaggagaga tgggtgagtg gcttaaacca gcagtttgct aaactgccgt atgggtaa
//
LOCUS       DAOOYG010000118         8167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9764_length_8167_cov_8.186637, whole genome shotgun sequence.
ACCESSION   DAOOYG010000118 DAOOYG010000000
VERSION     DAOOYG010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9764_length_8167_cov_8.186637"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..335
                     /gene="rpsT"
                     /locus_tag="PK288_09345"
     CDS             81..335
                     /gene="rpsT"
                     /locus_tag="PK288_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPM19170.1"
                     /translation="MANHRSAEKRIRQTETRKENNKYYGRTMRNALKKIRSTSNKEEA
                     AAMLPRLNSMLDRLARKNMIHKNKASNLKSSVARHVNSLK"
     gene            473..1156
                     /gene="radC"
                     /locus_tag="PK288_09350"
     CDS             473..1156
                     /gene="radC"
                     /locus_tag="PK288_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPM19171.1"
                     /translation="MSPKITDWAVEDRPREKFLKKGMSSLSNAELLAILICSGTKRSS
                     AVDLGRELLGMVNNNLSSLGKLSVSDLRKIRGIGPARAVTIAAALELGRRRNLAELPE
                     VSVIKCSKDVADIFRPLLSDLSYEEFWVLFLNRSNKVIDRIKLSQGGISGTVTDIRII
                     MKKAVENLASGIIVCHNHPSGNLAPSDSDTKITRKIREAGNLMDIQLLDHLIISDRDY
                     YSFADNGLI"
     gene            complement(1207..1746)
                     /gene="folK"
                     /locus_tag="PK288_09355"
     CDS             complement(1207..1746)
                     /gene="folK"
                     /locus_tag="PK288_09355"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPM19172.1"
                     /translation="MEREDKIVTGIFLGLGTNEGDRVENLKMALTLINEEVGDITALS
                     GVYETDPWGFESSNRFLNMVANVTTRLVPAALLKKTQEIEKKLGRVRSQVRFSSRSMD
                     VDILLFGNRVINQRKLTVPHPLIPERRFVLVPLCDLIPGRIHPVLKQTFSELLIQCRD
                     TGNVSRTEISLELPGKRKG"
     gene            2041..3810
                     /gene="sppA"
                     /locus_tag="PK288_09360"
     CDS             2041..3810
                     /gene="sppA"
                     /locus_tag="PK288_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPM19173.1"
                     /translation="MKEFLKYTLAAITGIFVASILLFIIMLSAISVMIATGNKQASIS
                     ANSVLVLKAGVSIPDRGDSGPFAGFDIINMTLTPTAGLNEILRNIKKAASDEKIKGIL
                     IENGISSSGWATVGEIRQALEEFRKSGKFVIAYSDYVLLQEGYYLSTAADRIFINPSS
                     TLEFKGLSGEVMFYKKALEKLGVDVQVTRHGKFKGAVEPFILNELSRENEEQIRAFIG
                     SIWKHVVGEISKSRGIDPVKINELADNLTGNLPATALENGLVDGLIYRDALIDTLKIR
                     SGLTTDDKIMLVPMTRYTKVPDPRKMVSAKNKISVIYASGTIAMGKGSQNNIGGDTYG
                     DLIRKERKDSTVKAIVLRVNSPGGNAIASDIIWKELELAAKVKPVIISMGNYAASGGY
                     YISAPGTRIFANPATITGSIGVFGLLPNAGKFLDQKLGISTETVNTNKNSDFPSVYRP
                     MTSYEKEVMQAGVDKIYFDFISKVSSGRNMSTEAVDNIGQGRVWSGADAMGIGLVDAA
                     AGLTASIDSAAALAGIEKYSLKELPVQEEPYARLISQLSGDSKIRILKSKFGESFRFV
                     EELMTIKELSGIQARMPYFIDIH"
     gene            3814..4908
                     /gene="lpxK"
                     /locus_tag="PK288_09365"
     CDS             3814..4908
                     /gene="lpxK"
                     /locus_tag="PK288_09365"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPM19174.1"
                     /translation="MGKKRSFLLYPPALVYGLVTAFRNYLYDSGFLTSREFSIPVICT
                     GNITVGGTGKTPHVEYLIELLGKEYRLAVLSRGYKRKSTGFRIVSPGSNASESGDEPL
                     QISRKFPGIIVAVDGDRVNGISNLVRKYPGLDLIIMDDGFQHRRMKPGFSILLSDYGR
                     PMYEDQMLPYGNLRESIKNIRRSDIVVITKSPKDISREETQKITRRLNEVHKKKVYFT
                     SLSYNHPVKLFGSIPEKNSFAGETGPEKAGAVIITGIASPESFIRFAENMFPETIHLD
                     FPDHHIYTWKDIRKIDRARNSLKSHRRLVITTEKDSVRLRELPDIPDHIRESLYYIPV
                     RVCFPAGGKDEFDKIILDYAGENKRDHGIS"
     gene            4871..5680
                     /locus_tag="PK288_09370"
     CDS             4871..5680
                     /locus_tag="PK288_09370"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPM19175.1"
                     /translation="MLEKINETTEFLKSAGFNGPQAGIVLGTGLGGLAAKIDNPLEVD
                     YSEIPNFPVSTVEGHEGKLIYGDFGGKKVVAMKGRFHYYEGYGPEDVVLPIRVLKYLG
                     IRHLFLSNAAGGVNPLFQIGDIMIITDHINLMPNPLTGKNDDRIGPRFPDMSEAYDRG
                     LIEKALLIAGKNGIRIRTGIYLATTGPTFETPAEYKYFRIIGADSVGMSTVPEVIVAR
                     HMGLPCFAVSIITDLGVEGKIEYITHEMVQREAEKAEAGMTRIMTELIAVT"
     gene            complement(5933..>8167)
                     /locus_tag="PK288_09375"
     CDS             complement(5933..>8167)
                     /locus_tag="PK288_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPM19176.1"
                     /translation="GNDDLLQLWRVTEGAFIPVIISNVNWQSDIGTSGYATIDVERSP
                     AGRWEMKIFRQNGSMADSCSESNLGSLTGDWFVICYRYTSARDRLLWIDDILIEGVFH
                     NDISPPALEKCEIAGKNSLVLSFSEEPGESVLQNGNFRIGNSDNQVVSVNKLTSRSVL
                     LEFEYYFTRGETCNLIIGSLCDKRENCTENVNVQFSYQPPGTGDIIFSEIMADPAPAV
                     SLPAREYLEIYNRTSGSISLKNWNLATETQNYPFPPVVIGPHEYMILCQMQDTSLFRA
                     YGRVAGMKNFPALTDAGKIIALTSSSGDLTDGIEYSSDWYRDILKSEGGWSLEIIDMS
                     YPFSSGDNWRASVSRTGGTPGRENSVAGCNPDVSFIGVTNVFPEDSMKISLSFSETVP
                     ELSAEMISIGMNGSGIQDLTSSDKLLRRFLVVPAEPLKRKQIYSLEADDRIRDYAGNT
                     MQVNSFMFGLPMQAATGDVIFSELLFNPLPGGADYIEFYNCSDKIIDVSRLGVVSVST
                     GTSDTSGLYYLSQEHRCLLPGEYYAITSGRRTVCEGFISSEPERIFEVSSMPSMPDKG
                     GHLILLNRQLDVIDEVFYDDAMHFSLLGTTEGVALERTRLSGLSSDRTLWHSASETSG
                     WGTPGAPNSAVPEHMFNDDQVTLSTTRITPDNDGNQDWLEIGMKFRGNGNIVSVAVFD
                     EYGGFVRKLADNLLAGTEATIMWDCTVDNEKLVSPGVYILLVTVFNDAGKVKKWKKVC
                     TVIR"
BASE COUNT         2314 a         1901 c         1890 g         2062 t
ORIGIN      
        1 acgtcaggtt ttcatcctgg taacaggggt tcgattcccc tatggactac aaaaaacaaa
       61 gggagacaaa ttagaatatc atggctaatc acaggtcggc agaaaaaagg ataagacaga
      121 ccgaaacgag aaaggagaat aacaagtatt acggtaggac catgcgtaat gcactcaaga
      181 agatacgcag cacttcaaac aaggaagaag cggcagcaat gctgcccagg ctgaattcca
      241 tgcttgacag gctggccagg aaaaacatga tacacaaaaa caaggcttca aacctgaaat
      301 catcagttgc aaggcacgtc aattccctga aataagcagg tcaaaaacat atcacaaaaa
      361 ggtgtggtca tatgatcaca ccttttttgt ttcatcgggc atgagaaatt tttcatgacc
      421 ggctgcatca tcccgggatt tttattattt tcatgaataa atatttcgct gcatgtcacc
      481 taaaatcacc gactgggccg tcgaggacag gcccagggaa aaatttctca aaaaaggcat
      541 gtcctccctg agtaatgccg aattacttgc gatcctgatc tgttcgggaa caaaaaggag
      601 ttctgccgtt gatctgggca gggaactgct tggaatggtt aacaataacc tcagctcact
      661 tggaaaactg tcagtttccg atctgaggaa aataagagga atcgggccgg caagggcggt
      721 tacaattgct gcggccctcg agctgggacg aagaaggaac ctggctgaac ttcctgaagt
      781 ttccgtgata aaatgctcga aagatgttgc tgacatcttt aggccgctgc tttccgatct
      841 gtcatacgag gaattctggg tgctcttcct gaaccggtcg aacaaggtca tcgacaggat
      901 aaaactgagc caggggggta taagcggtac cgttaccgac atcaggataa ttatgaaaaa
      961 ggctgtggaa aacctggcct ccggtattat cgtctgccac aatcatcctt caggcaatct
     1021 ggccccgagt gactccgata caaaaatcac ccggaaaatc agggaggccg gaaacctgat
     1081 ggacattcag ctgctcgacc atctgataat atcagaccgg gattattaca gctttgccga
     1141 caacggcctt atatgaacct gagggatttc ctatcttatt gctgaaatga tcgagccgcc
     1201 ggtcattcag cctttccttt taccaggtag ttccaggctg atttctgtcc ggcttacatt
     1261 gccggtatcc cgacattgta tcagcagttc ggaaaatgtt tgtttcagca ccggatggat
     1321 tctcccggga ataagatcgc ataaagggac gagaacaaac cttctttcag gaattagtgg
     1381 atggggaacc gtaagtttcc tttgatttat cacccggttg ccgaaaagca gtatatcaac
     1441 atccatgctt cgtgagctaa aacgcacctg gcttctgacc cgtccaagtt ttttttcaat
     1501 ttcctgcgtt tttttcagga gggctgccgg taccagccgg gtagtaacat ttgcaaccat
     1561 gttcagaaac cggttgctgc tttcaaaccc ccagggatca gtttcataga caccggataa
     1621 cgccgttata tcacctactt cttcgttaat cagagtaagt gccattttca ggttttcaac
     1681 cctgtcgcct tcattggtac caaggcccag gaaaatgcct gtaactattt tatcttcgcg
     1741 ttccaaataa tgaaccaggt ttcttctgaa cttattctaa tcatctgatc tgcaggaact
     1801 tcctgcagat ttttaagcca gggatcagag tccgtgaatt aatcaggcca ctattcctga
     1861 aataagtgca atttacaaaa tgaatcggaa ggtttcgaaa aagccgtggt ttcaggggac
     1921 tcatttcaca ttcccgattt cgtatcggtg agcatattaa tataaatctg tgaaccgtaa
     1981 cagaacaaat acaatgtttt taatatcttt gacattcatt tccttaactt ttaacaacat
     2041 atgaaagagt ttctgaaata tacactggct gcaatcacag ggatattcgt tgcatccatt
     2101 ctgttgttca taatcatgct ctcggccatc agtgtcatga tagccacagg aaataaacag
     2161 gcatccatat cagccaattc ggtacttgtt ctgaaagccg gggtcagcat tcccgacagg
     2221 ggagactcag gtccctttgc cgggtttgac ataatcaata tgaccctaac cccgaccgcg
     2281 ggcctgaacg aaatcctccg aaacatcaaa aaagctgcat ccgacgaaaa aataaaaggt
     2341 attctgatcg agaacggtat ctcatcttcc ggctgggcaa ctgtcggaga gataaggcag
     2401 gctcttgagg aattcaggaa aagcgggaag tttgtaattg cctattcgga ttatgtgctc
     2461 ctgcaggaag gttattatct ttccacggca gccgacagga tattcataaa tccctcatca
     2521 actttagaat tcaaggggct tagcggagag gttatgtttt acaaaaaagc cctggaaaag
     2581 ctgggcgtgg atgttcaggt tacccggcac gggaaattca aaggtgcagt tgagcccttc
     2641 atccttaatg agctgagcag ggaaaatgaa gaacagataa gggctttcat cggatccatc
     2701 tggaaacacg ttgtcgggga aatatcgaag tcaaggggta ttgatcccgt gaagatcaac
     2761 gagctagccg ataatctgac cgggaatctg cctgcaacag ccctggagaa cgggctggtt
     2821 gacggactta tttacaggga tgccctgatc gataccctga aaatccgttc gggtcttacc
     2881 accgatgaca aaataatgct tgtccccatg accagataca ccaaggtacc tgacccccgg
     2941 aaaatggtgt ctgcaaaaaa caagatctcg gtcatttatg cttccggcac aattgctatg
     3001 ggaaaaggca gccagaacaa catcgggggt gatacatatg gtgatctgat caggaaagaa
     3061 agaaaggatt cgactgttaa agcgatagtt ctaagagtca actcaccggg aggtaatgca
     3121 attgcctccg acattatctg gaaagagctt gaacttgctg ctaaagtcaa gcccgtgata
     3181 atctcaatgg gaaattatgc agcatcgggg gggtattaca tttcagcccc cggcaccagg
     3241 atctttgcca atccggcaac aattaccgga tcaatcgggg ttttcggact tctgccaaat
     3301 gcaggaaaat ttcttgatca gaagcttgga atctctaccg aaacggtcaa tacaaacaag
     3361 aactcagatt tcccgtcagt ttaccggccc atgacttctt atgagaagga agttatgcag
     3421 gctggtgtcg acaagatcta ttttgatttt atcagtaagg tttcatctgg caggaatatg
     3481 agtaccgagg ctgtggacaa tatcggacag ggtcgtgtat ggagcggagc cgatgcaatg
     3541 gggataggac ttgtcgatgc tgcggccggc ctcaccgcat caatcgatag tgccgccgca
     3601 ctggcgggta tagaaaaata ctccctgaaa gaactccccg tgcaggagga accttatgcc
     3661 agactgatat cccaactcag cggtgactca aaaatccgga ttcttaaaag caagttcggg
     3721 gaatctttca gatttgttga ggagctcatg acaatcaaag aactgtcggg aatacaggca
     3781 agaatgccat actttattga tatccactaa ggcatgggca agaaacgaag ctttctgtta
     3841 tatcctcccg cacttgttta cggtcttgtt acagcattta gaaattacct gtatgactca
     3901 ggttttctga cttcaaggga attcagtatt ccggttatct gtacaggcaa tatcactgta
     3961 ggcggaacgg gaaaaacacc tcatgttgaa tatctcattg aactgctcgg aaaagaatac
     4021 cggctggctg tattgagcag gggttacaaa agaaaatcaa ccggcttcag gatcgtctca
     4081 cccggttcca atgcaagtga gtcaggtgat gaaccgcttc agatctcccg taaatttccc
     4141 ggaataattg ttgccgttga cggcgaccgc gtaaatggca tcagcaatct tgtccggaaa
     4201 tatcccggcc ttgacctgat aatcatggat gacggttttc agcacaggag aatgaaaccc
     4261 ggattttcaa tacttctttc agattacggc cgacccatgt atgaagatca gatgcttccc
     4321 tatggaaatc tcagggaaag cattaaaaac atccgccgtt ccgatatagt tgtaattacc
     4381 aaatctccca aggatataag ccgtgaggaa acgcaaaaaa taacacgccg gctgaatgaa
     4441 gtgcataaga aaaaggtata ttttacttca ctttcatata atcatcccgt aaagctgttt
     4501 gggagcatac cggaaaaaaa tagtttcgcc ggtgaaaccg gtccggagaa agcaggagca
     4561 gtaataatca ccggaatagc atccccggaa tcatttatca gatttgcgga aaatatgttc
     4621 cctgagacca tacatctgga ttttcctgat catcatattt acacatggaa ggacatcagg
     4681 aaaattgaca gggcccgaaa cagccttaaa tcgcaccgca ggctggttat caccaccgaa
     4741 aaggacagtg tgaggttaag agaactcccg gatattccgg atcacatcag ggaatcgctt
     4801 tattacatcc cggtcagggt atgctttccg gccggcggga aagacgaatt tgacaaaata
     4861 atacttgact atgctggaga aaataaacga gaccacggaa tttcttaaat cggcagggtt
     4921 caacggaccc caggcgggta tagtattggg aacggggctg ggaggcctgg ctgcaaagat
     4981 tgataaccct ctggaagtcg attacagtga aatcccgaat ttccctgttt caactgttga
     5041 agggcacgaa ggcaaactta tttacggtga tttcggcggg aaaaaagttg tggccatgaa
     5101 gggaaggttc cattattacg agggatacgg gccggaagat gttgtccttc ccataagggt
     5161 cctgaaatat ctcggaatac ggcatctttt tctgtccaat gccgcaggcg gcgtgaatcc
     5221 gttgtttcag atcggcgata taatgataat caccgatcac atcaacctga tgccgaatcc
     5281 gctcacagga aagaatgatg acaggatagg gcccagattt cccgacatga gcgaggcata
     5341 tgacaggggg ctaattgaaa aggcactcct tatagccggg aaaaatggga tcaggatccg
     5401 gacgggtatc taccttgcaa ctacaggacc taccttcgag accccagctg aatataaata
     5461 cttccgcata ataggggcag attcagtagg gatgtcgacc gtgcccgaag taattgttgc
     5521 aaggcatatg gggctcccct gctttgcagt cagcatcatt acagatctcg gagttgaggg
     5581 aaagatagaa tacatcaccc atgaaatggt tcagagggaa gcagaaaagg ccgaagccgg
     5641 gatgaccagg atcatgaccg agctgattgc agtaacctga cctctttcac ctggttccac
     5701 tccgataacc tgattccctg cacatatggc cggtctgact tcccaattaa ccccataaag
     5761 atttgtggaa gccaagggga actatcgtcc tgtctgctat tcccatttga cttccctttt
     5821 ccttacctcg caggttcttc agaagtttaa aattacaatg cagcatttgc aggcaataca
     5881 tgggagccgg gcagaaacag gtcccggacc gatcccacag gaaggcaggc catcacctga
     5941 tgacagtaca gacttttttc cattttttca ccttacccgc atcgttaaaa acggtaacca
     6001 gcaggatata aactcccggg ctgaccagct tttcattatc cacggtacaa tcccacatta
     6061 ttgttgcctc tgttccagca agcagattat cagccagttt tctgacaaat ccgccgtatt
     6121 catcaaaaac agcgacagaa acaatattcc cgttacccct gaatttcata ccgatttcaa
     6181 gccagtcctg attaccgtcg ttgtcgggtg ttattcttgt tgttgacagt gttacctgat
     6241 catcgttaaa catatgctca ggtacagctg aattgggagc tccaggtgtt ccccacccgg
     6301 aagtttctga agctgagtgc cagagggtcc ggtctgatga cagtccggat aaacgtgttc
     6361 tctcaagtgc aacgccttcg gtggttccga gcagggagaa gtgcatagcg tcatcataga
     6421 aaacctcatc gatcacgtca agctgcctgt tgagcaagat aagatgacct cccttatcgg
     6481 gcattgaagg cattgaggat acttcaaaga tcctttccgg ttcggaggaa atgaatcctt
     6541 cgcatacagt tcttctgccg gatgttatgg cataatattc tccgggaagc aggcatctgt
     6601 gctcctgtga aagataatac agacccgacg tatcggatgt tcccgtactc accgaaacaa
     6661 ctccaagcct cgagacatct attattttat ctgaacagtt ataaaactca atataatctg
     6721 ctcctcccgg caaaggattg aaaagaagtt cactgaaaat aacatcacct gttgccgcct
     6781 gcatggggag cccgaacatg aaggaattga cctgcatggt gttgcctgca taatccctga
     6841 tcctgtcatc ggcttcaagg gaatatatct gtttcctttt aaggggttcc gccggaacaa
     6901 ccaaaaatct tctgagaagt ttgtctgaag aagtaagatc ctgaataccg gatccgttca
     6961 ttccgatgct tatcatctca gccgaaagct ccggaacagt ttctgagaat gagaggctta
     7021 tcttcatgct gtcttcaggg aaaacattgg tcacaccaat aaaactgacg tcaggattgc
     7081 agcctgcaac tgagttttcc ctgcccggtg taccccccgt ccttgaaacc gaagccctcc
     7141 agttgtcccc ggatgagaag ggataactca tgtcaatgat ctcgagcgac cagcctcctt
     7201 cagatttcaa aatatccctg taccagtctg atgaatattc gataccatct gtcagatcgc
     7261 cggaactgct ggttagtgca attatttttc cggcatcggt aagtgcggga aagtttttca
     7321 ttcctgccac ccggccataa gccctgaaca gggaagtgtc ctgcatctga cataatatca
     7381 tatattcatg tggtcctata acgaccggcg gaaaagggta attctgcgtc tccgttgcaa
     7441 gattccagtt cttcaggctg atgctcccgg atgtcctgtt gtaaatctcg aggtactccc
     7501 ttgccgggag tgaaacggca ggagcaggat cggccattat ttcagaaaag attatatcgc
     7561 cggtacccgg tggctgataa ctgaactgta cattgacgtt ttcggtgcaa ttttcccgct
     7621 tgtcacataa agaacctatg atcaggttgc aggtttcccc tctcgtaaaa taatattcaa
     7681 actccagcaa taccgatctt gatgttaatt tattaacact caccacctgg ttgtcagaat
     7741 tgccgatgcg gaaattgcca ttttgcagca ctgattcgcc tggttcttca ctgaacgaca
     7801 aaacgagtga gttcttcccg gcaatttcac atttctcaag ggcaggagga gatatatcat
     7861 tatgaaaaac tccctcaatc aggatatcgt cgatccataa cagccggtcg cgggcagagg
     7921 tgtacctata gcatatcaca aaccagtcgc cggtcagtga tcccaggtta gattctgaac
     7981 aggaatctgc catgctgcca ttctggcgga atattttcat ttcccagcgg ccagccggtg
     8041 aacgttcaac atctatggtg gcataccccg atgttcctat gtcggactgc cagttgacat
     8101 tgcttattat caccggaata aatgcgcctt cagttactct ccagagttga agcagatcgt
     8161 catttcc
//
LOCUS       DAOOYG010000119         8116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9858_length_8116_cov_5.625977, whole genome shotgun sequence.
ACCESSION   DAOOYG010000119 DAOOYG010000000
VERSION     DAOOYG010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9858_length_8116_cov_5.625977"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="PK288_09380"
     CDS             <1..1364
                     /locus_tag="PK288_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPM19177.1"
                     /translation="FHHALKVHKDLCTGCTHCMIVCPTRAIRIRKGKAIIIADRCVDC
                     GECYRACPVSAIGVEQDDLNLILKFPVRVLLIPGVFIGQFSHKIPLSSIYGALGKIGF
                     THVYHVENTVDMVLDAYDEYALQNESRPLISSFCPAVVRLIQVRFPSLVDNIMKVNPP
                     VDMSAIYYREKLRSSGIPEQNTGLFYATPCAAKIAAIKSPVGETESVVTGVINTNYLY
                     NKVYQLLQKGEAFNEIPVTDDLLSSRGILWSLTRGESSHRKKRSLSVDGIRNVTEILE
                     SVENDKLDNIDFLELRACDEGCAGGILLPGNRFVTVERLRKLASDFSKSETDSRRKLT
                     EGFINLKEQSFLGKIEPRNIEKLDEDFTVAMKKMKRKRRLMCFLPGFDCAACGSPDCQ
                     TLAEDTVRGYGQLSHCVFMQQQMVKQKLMSQEKAFSLTEEIWGAERLEKNCNKIGAEN
                     ENI"
     gene            1351..1686
                     /locus_tag="PK288_09385"
     CDS             1351..1686
                     /locus_tag="PK288_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPM19178.1"
                     /translation="MKISEIVNRLNLKVVAGADGLGAEVTGGYVSDLLSDVIGHAAEG
                     NIWITLQTHQNIVAVASLKELSAIIVVKGSSPAADTIRKSNEENIPLLSTGLDTFRIA
                     GELYNLLLK"
     gene            1683..2417
                     /locus_tag="PK288_09390"
     CDS             1683..2417
                     /locus_tag="PK288_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPM19179.1"
                     /translation="MKIYRADLHIHTLLSPCGELSMSPARIIETAKRRNLDIIGISDH
                     NTTRQSNLVRTLGEKKGIYVLRGAEVTTREEIHCLVFFEENDSLSRFQELLDDTLPCI
                     QNNPLIFGDQVVVDENENIVYTEERLLTNAIKLSLGELEAWVHANDGIFIPAHIDRMK
                     NSIYSQLGFLPENLKADALEVSKRSSPGQFRINHPETVGYTLITNSDSHYCESIGTVF
                     NNFHMEGTTFEEIRMALAGEKGRKVD"
     gene            2392..2979
                     /locus_tag="PK288_09395"
     CDS             2392..2979
                     /locus_tag="PK288_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM19180.1"
                     /translation="MRKEERLTDMITLALNILDIVQNSISAMATEIAVEVTESAAVDL
                     MKITVKDNGRGMSQDLLSRVTDPFVTTRKTRRIGLGIPLLKYHANLAGGDLEIQSGEG
                     IGTTLSATLKLQHPDRQPLGDLAGMMTILMTANPGIDFLYTHKTDMGEYCFSSREVME
                     YLGITDFSDVSLLTDIREMMNNNLEDIGASGFNGE"
     gene            3071..3472
                     /locus_tag="PK288_09400"
     CDS             3071..3472
                     /locus_tag="PK288_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPM19181.1"
                     /translation="MSQIKSLADLRKKREEIKAEMDTRIKAIDPENHVQIKVAMATCG
                     IAAGAKEIMEFFVEQLDRRNIQGVVTQTGCMGYCYAEPTVEITLPGREPVVYGFVDLK
                     RADQIIEKYIKEGELIDGIIPVNYETIDSKE"
     gene            3484..5277
                     /locus_tag="PK288_09405"
     CDS             3484..5277
                     /locus_tag="PK288_09405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPM19182.1"
                     /translation="MSKYSMHLLVCGGTGCHASESDAILWNLRDELEAKGLSDKVQVI
                     LTGCFGFCEKGPIVKVMPDNTFYVQVKPDDAQAIVEEHIIKGRKITRLLYHDPKTKEP
                     VGDSKHMGFFKKQLRIVLRNCGFINPENIDEYIARDGYEALGQVLSEMTPEQVIEIIK
                     DSGHRGRGGAGFPTGFKWEIARRNKSDEKYVVCNADEGDPGAFMDRSVLEGDPHSVLE
                     AMTICGYSIGASKGLIYVRAEYPLAIHRLQVAIRQAHEYGLLGKNILGTDFSFDISLR
                     LGAGAFVCGEETALIHSMEGHRGEPTIKPPFPAESGYRGKPTNVNNVETFASIPVIIL
                     KGAEWYKRIGTEKSKGTKVFALAGKINNVGLIEVPMGTTLREVIFEIGGGIRNGKKFK
                     AVQTGGPSGGCLTEKHLDVPIDFDNLVAAGSMMGSGGMIVMDEDDCMVSVAKYFLDFT
                     VEESCGKCSPCRIGNKRLYELLNHICEGKGNVSDLDRLRNMSLVIKDTSLCGLGQTSP
                     NPVLSTLDNFMDEYIAHVRDKKCPAGKCKDLMEYYIIPENCVGCTACARVCPVNAISG
                     ERKEIHYINPEVCIKCGACMEKCKFDAIIIR"
     gene            5289..7067
                     /locus_tag="PK288_09410"
     CDS             5289..7067
                     /locus_tag="PK288_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPM19183.1"
                     /translation="METVRLTIDNKEIEVPKGTTIYKAAKQLGIEIPTLCYLHLDDLN
                     IENKPGGCRICVAEVQGRRNLAPTCSTNCENGMVVKTHTTRVLNARRTVMEFILSDHP
                     TDCLVCPKSGTCELQNMAHLLGIREIPGAEYAEMSKYKPDTSPSIIRDLDKCIMCRRC
                     EMMCNDVQTVGALSAVNRGFMAAVAPAFEQNLEHSTCTYCGQCVAVCPTGALTEVDHT
                     PEVLRALADPTKTVIVQTAPAVRAALGEAFGMAPGTLVTGKMVAALRALGFDYVFDTD
                     FGADLTIMEEGTELLDRIKRHLSGDTTVRLPLLTSCCPGWVNFFEEYFPDLKDVPSSA
                     KSPQQMFGAIAKSYFAEKIKVDRENLVVVSIMPCLAKKYECQREEFSVNGNRDVDYSI
                     STRELAAFIKQANLDLNNLEDEEFDAPLGESTGAGVIFGATGGVIEAACRTAYEMYTG
                     KKLDRIDFTELRGLEGVRSATIDFDGLPLNIGIAHGLGNARKLLDDVRSGKSQFHAIE
                     VMACPGGCIGGGGQPAHHGNSEIIKARAAAIYREDSKKPLRKSHENPFIIKLYEEYLG
                     HPNSEKAHHLLHTRYFDKKKKVIVKQ"
     gene            7104..7586
                     /gene="nuoE"
                     /locus_tag="PK288_09415"
     CDS             7104..7586
                     /gene="nuoE"
                     /locus_tag="PK288_09415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPM19184.1"
                     /translation="MSSIKIELRQEDVNKIKEICTSFNNDPQELINILHSCQEHFGYL
                     PAEVQEVISSETDVPVAQIYGVVTFYSFFTMTPKGKHPISICMGTACYVRGAEKVLEE
                     FKKELNLQVGQTTKDGKFSLSNLRCVGACGLAPVVLIGDKTYGRVAPDDVKKIIKEYE
                     "
     gene            7718..>8116
                     /locus_tag="PK288_09420"
     CDS             7718..>8116
                     /locus_tag="PK288_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19185.1"
                     /translation="MNVHKGVNHKMAVVPLIAGFLFFAVSELTAQEKPCIVKHPALSE
                     KYSGDCKNGLANGKGAAQGIDKYHGQFRNGLPHGKGTYIWADGTHYTGQFKNGLKDGK
                     GTLVTKDSTVTGYWQEDRYIGKRIVAPYQIT"
BASE COUNT         2443 a         1775 c         2031 g         1867 t
ORIGIN      
        1 aatttcatca cgctttaaag gtgcacaagg atctttgcac cggctgtacg cattgcatga
       61 tagtctgccc taccagggcc atccgcatca gaaagggcaa ggcgatcata attgccgaca
      121 ggtgcgttga ttgcggcgaa tgctacaggg cctgcccggt gtcagcaata ggtgtcgagc
      181 aggatgatct caacctgata ctgaaattcc cggtaagagt gctcctgatc ccgggtgtat
      241 ttatcggcca gttttcacat aaaataccac tttcatcgat ctacggagct ctcggtaaaa
      301 taggtttcac ccatgtttat cacgtggaaa ataccgtaga catggtcctg gatgcttatg
      361 acgaatatgc cctgcaaaac gaaagccgcc cgcttatctc ttctttctgc ccggcagtgg
      421 taagacttat ccaggtaagg ttcccttccc tggtcgataa cattatgaag gtgaaccctc
      481 ctgtcgatat gtctgcaatt tattacaggg agaaactcag gagttccggt attcccgaac
      541 aaaacacagg tctgttctac gcaacaccct gtgccgccaa aattgcagca atcaagagcc
      601 cggtcggaga aaccgaatcg gtggtgaccg gagtaatcaa taccaactac ttgtataaca
      661 aggtttacca actcctccag aaaggggaag ccttcaacga aatccctgta acagacgacc
      721 tcctgagttc caggggaata ctgtggagcc ttacccgggg agaatcttct caccggaaaa
      781 aaaggtcgct ctcggtcgac gggatcagga atgttactga aatactggag agcgttgaaa
      841 acgacaaact tgacaatatt gattttctcg aactgagagc ctgcgatgaa ggatgtgcag
      901 gcggtattct cctgccgggc aaccgttttg tcactgtgga aaggctccgc aagcttgctt
      961 ccgatttctc aaaaagcgaa acagacagca gaagaaaact cacggaagga tttatcaatt
     1021 taaaggaaca aagcttcctg ggtaaaattg aacctagaaa cattgaaaag ctcgatgagg
     1081 atttcactgt ggccatgaag aaaatgaaaa ggaaacgcag attgatgtgc ttccttcccg
     1141 gtttcgattg tgctgcatgc ggttcacctg actgccagac acttgcagaa gatacggtcc
     1201 ggggctacgg gcagctttcg cattgcgttt tcatgcagca acagatggtc aaacagaagc
     1261 tcatgagtca ggaaaaagca ttcagcctga ccgaggagat ctggggagct gaaagactgg
     1321 aaaagaactg taataaaatc ggcgcggaaa atgaaaatat ctgaaatagt aaatcgcctg
     1381 aacctcaaag tagtagccgg agccgacgga ttaggagcgg aagttaccgg aggttatgtt
     1441 tccgatctcc tcagcgatgt cattggacat gcagctgaag ggaatatctg gatcacactt
     1501 cagacacatc agaacattgt tgccgttgcc agcctgaagg aactttcggc aataattgta
     1561 gtaaaaggtt cttctcccgc tgcagatact ataaggaaaa gcaatgagga aaatataccc
     1621 ctgctttcta ccggcctgga tacattcaga atagccgggg aattatataa tttactttta
     1681 aaatgaagat ataccgggca gacctgcata tacacacctt gctttcaccg tgcggtgagc
     1741 tgagcatgag cccggcaaga ataattgaga cggccaaacg caggaatctt gacattatcg
     1801 gaataagcga tcacaatacc acaaggcaga gcaaccttgt cagaacgctt ggcgaaaaaa
     1861 agggaatata tgtgctgcgg ggcgctgaag taacaacaag agaggagata cattgcctcg
     1921 tgttctttga agaaaatgat tccctcagcc ggttccagga attgctggac gataccctgc
     1981 catgcattca gaacaacccg ctgatatttg gtgatcaggt tgtggtggat gaaaatgaga
     2041 atattgttta taccgaggaa agattactga caaatgccat aaaactttca ctcggagaac
     2101 tggaagcatg ggtacatgcg aatgatggaa ttttcatccc tgcccacatc gacagaatga
     2161 aaaacagcat ttacagccaa ctcggatttc tgccggaaaa ccttaaggca gatgcactgg
     2221 aggtttcaaa aagaagttca cccggacagt tcagaataaa ccacccggaa actgtgggtt
     2281 acacgctgat tacaaattct gattcacatt actgcgagtc aataggaact gtattcaaca
     2341 acttccatat ggaagggaca acatttgagg aaatacgaat ggcactggcg ggtgagaaag
     2401 gaagaaaggt tgactgacat gataacactg gcattaaata ttcttgacat cgttcagaat
     2461 tccataagcg caatggccac ggaaattgca gtggaagtaa cggaatctgc cgcagttgac
     2521 ctgatgaaga tcacagtgaa ggacaatggc aggggaatgt cgcaggatct gttatccagg
     2581 gttaccgacc cgtttgtgac aaccaggaaa accaggcgga ttgggctggg cattcctctg
     2641 ctgaaatatc acgcaaatct ggccggaggc gatctggaga ttcagtccgg tgaaggtatt
     2701 ggtaccaccc tgagcgcaac cctgaaactt cagcatcctg accgccagcc attgggtgat
     2761 ctggccggaa tgatgacaat tctcatgacg gccaatccgg gaattgattt tctttatacc
     2821 cacaaaaccg atatggggga atactgtttt tcatccaggg aagtcatgga atatctggga
     2881 attacagact tcagcgatgt ttcgctgcta actgacataa gggaaatgat gaacaataac
     2941 ctggaggata tcggggcatc ggggtttaac ggggaataga tgaaaaattg tcgtaaaatc
     3001 aattaaacca ggataattgc aggtatatta gataaattaa tcaatttgaa ttaactaaac
     3061 aggaacgagc atgtcacaaa tcaagtctct cgccgatctc aggaagaaac gcgaagaaat
     3121 aaaagcagaa atggacacca ggatcaaggc tattgatcct gaaaatcatg ttcagatcaa
     3181 ggttgcgatg gcaacctgtg gtattgctgc aggagctaag gaaataatgg aattttttgt
     3241 tgaacagctc gacaggagga atatccaggg agtggttaca caaaccggat gcatgggata
     3301 ttgctatgcc gagcccactg ttgaaattac tcttccgggc agagaacctg tggtttacgg
     3361 atttgtcgac ctgaaaaggg cagaccagat aattgagaaa tatataaagg agggtgagct
     3421 tatcgatggt atcatcccgg taaattatga aacaattgat tctaaagaat aggaggatag
     3481 caaatgtcaa aatacagtat gcatcttctg gtatgtggcg gaacaggctg tcatgcttct
     3541 gaaagcgatg caattttatg gaatttaaga gatgagctgg aggcaaaggg attatcggac
     3601 aaggtccagg tgatcctgac cggctgcttc ggattctgtg agaagggccc cattgttaag
     3661 gtgatgcccg ataatacatt ttatgtccag gtaaaacctg atgatgcaca ggccattgtc
     3721 gaagaacaca ttattaaggg aagaaaaatt accaggcttc tctatcatga tccgaagaca
     3781 aaagagccgg taggtgattc aaagcacatg ggatttttca agaagcagct caggatagtg
     3841 ctcaggaact gcggctttat aaatccggaa aacattgatg aatatattgc aagggacgga
     3901 tatgaagccc tgggacaggt gctttccgaa atgacgccgg aacaggtcat tgaaataata
     3961 aaggattccg gtcacagggg ccgcggcggg gccggattcc ctacaggatt caaatgggaa
     4021 atagctcgca ggaacaagtc cgacgagaag tatgttgtct gcaatgccga tgaaggagac
     4081 cccggtgctt tcatggacag atcggttctt gaaggcgatc cccattccgt ccttgaggcc
     4141 atgactatct gcggttacag tatcggagca tcaaaaggac tgatctacgt ccgcgccgaa
     4201 tatccgctcg ctattcacag actccaggtc gcaatcaggc aggcacatga gtacgggctc
     4261 ctgggcaaga atattcttgg aactgatttc agttttgata tttccctcag actcggtgcg
     4321 ggagcattcg tatgcggaga ggaaaccgca ctgatacact ccatggaagg ccacaggggc
     4381 gaacctacaa taaagccgcc gttcccggcc gaatcaggat atcgcgggaa gccaacaaac
     4441 gtgaacaatg ttgaaacttt tgcctccatt cctgtaataa tacttaaagg tgctgaatgg
     4501 tataaacgca taggcacgga aaaatcaaaa ggcacaaagg tttttgcact ggccggtaaa
     4561 atcaacaatg tgggattgat cgaggttccg atgggaacca cgctgaggga ggtaatcttc
     4621 gagatcggcg gaggaatcag aaacggcaag aaattcaagg ctgtccaaac cggaggacca
     4681 tcaggaggct gcctgaccga aaagcatctt gacgtgccaa ttgatttcga caatcttgtg
     4741 gctgcagggt caatgatggg atcgggagga atgatcgtaa tggacgagga tgactgcatg
     4801 gtgtcagtgg caaagtattt cctcgatttc acagttgaag aatcgtgcgg caagtgctct
     4861 ccgtgcagaa taggaaataa acgactgtat gaactcctga accatatctg cgaaggaaag
     4921 ggcaatgtga gtgatctcga ccggttgcgc aacatgagtc ttgtcattaa ggatacttcc
     4981 ctgtgcggac tgggtcagac ctccccaaac ccggtgctgt ccaccctcga caatttcatg
     5041 gatgagtaca ttgcacacgt gcgggataag aagtgtcctg ccgggaaatg caaggacctg
     5101 atggaatact atattatacc cgagaactgc gtgggctgta ctgcatgcgc aagagtatgc
     5161 cccgtaaatg caatatccgg tgaacggaag gaaattcatt acattaatcc tgaagtctgc
     5221 ataaaatgcg gtgcgtgcat ggagaaatgt aagtttgatg caataattat cagataatgg
     5281 gaaaaatgat ggaaacagta agacttacaa tagacaataa ggaaattgag gtaccaaaag
     5341 gtacaactat atataaggca gcaaaacagc ttggaataga gatccccacg ctctgctatc
     5401 tccatctcga tgatctcaac attgagaata agcctggcgg atgccgtata tgcgttgcgg
     5461 aagtacaggg cagacgcaat cttgccccca catgctccac aaattgcgag aacggcatgg
     5521 ttgtgaaaac ccacaccacc agggtgctta acgcaaggcg gaccgtaatg gaatttattc
     5581 tttccgacca tcctacggat tgcctagttt gccccaaatc aggcacatgc gaactgcaga
     5641 atatggcaca cttgctcggc ataagggaga taccgggtgc ggaatatgcg gagatgtcta
     5701 aatataagcc cgacacctct ccttccataa tcagggatct cgacaaatgc ataatgtgca
     5761 ggagatgcga gatgatgtgc aatgatgtgc agactgtggg tgccctttct gcagtgaacc
     5821 gcgggttcat ggctgcggtg gcacctgcct tcgagcagaa cctcgagcat tccacctgca
     5881 cctactgcgg acagtgtgtg gcagtatgcc cgacaggtgc acttactgag gttgatcata
     5941 caccggaggt actgagagca cttgcagatc cgaccaaaac ggtaatagtc cagaccgcac
     6001 ctgccgtgcg cgcagcattg ggagaagcat tcggaatggc tcccgggacc ctggtgacgg
     6061 gcaaaatggt tgcagcactg agagcactgg gattcgacta tgtttttgat accgactttg
     6121 gagctgacct cacaattatg gaagaaggca ccgagctgct cgacaggatc aaaaggcatc
     6181 tcagcggcga taccaccgta agactcccgt tgcttacatc ctgctgcccc ggatgggtga
     6241 acttcttcga ggaatatttt cccgacctta aggatgttcc ctcatcggcc aaatcacccc
     6301 agcagatgtt cggagcaatt gccaaatcat acttcgctga aaaaatcaag gttgacaggg
     6361 aaaaccttgt cgttgtttca atcatgccct gtctggcaaa aaaatatgaa tgccagagag
     6421 aggaattctc cgttaacggg aacagggatg tcgactattc aatctcaacc agggagcttg
     6481 cagcatttat caagcaggca aaccttgatc tgaacaacct tgaagatgaa gaattcgatg
     6541 caccgctcgg tgaatcgaca ggcgcaggag ttattttcgg tgcaacgggc ggcgtcattg
     6601 aagctgcatg cagaactgct tatgaaatgt atacaggcaa aaaacttgac aggatcgatt
     6661 tcactgaact caggggcctg gaaggtgtcc gttccgctac catcgacttc gacggccttc
     6721 cgctgaatat cggtattgct cacggacttg ggaatgcaag gaagcttctc gatgacgtac
     6781 gttcaggcaa atcccagttc catgccattg aggtcatggc ctgcccggga ggatgtatcg
     6841 gaggcggagg ccagccggct catcatggca attccgaaat aataaaagcc cgcgctgccg
     6901 caatctacag ggaagacagc aaaaaacccc tgcgcaaatc gcacgagaat ccgttcataa
     6961 tcaaacttta tgaagaatat ctcggtcatc ccaacagcga aaaggcccat cacctgcttc
     7021 ataccaggta ttttgacaaa aagaagaagg tcattgtaaa acagtaatta aaaccgttat
     7081 ggacaatata aaaatcaatt gccatgtcaa gtattaaaat agaattgaga caggaagatg
     7141 tcaataaaat aaaagaaatc tgtacttcat tcaataatga cccacaggaa ctgatcaata
     7201 tccttcattc ctgccaggag catttcggtt atctgccggc agaagtacag gaggtcattt
     7261 cgtccgaaac tgatgttcct gttgcgcaga tctacggagt cgttacgttc tattcattct
     7321 ttaccatgac tccaaaagga aaacacccta tcagcatttg catgggcacg gcttgttatg
     7381 taaggggtgc cgagaaagtg cttgaggaat tcaagaaaga acttaacctc caggtgggac
     7441 agacaacaaa agacggcaag ttctcccttt caaacctgag atgcgtcggt gcatgcggtc
     7501 ttgcacccgt agtgctaatc ggggacaaaa cctacggcag ggtggccccc gatgatgtga
     7561 aaaaaataat caaggaatat gagtgattct ttaaatgatt gatttttagg ttgaaaaccg
     7621 gggttgacat acaatcccgg ttttttatcc cttgcccgtt atgaaaaata atattattac
     7681 ctttagagat cagaatatta attcaaataa gttgaaaatg aatgttcaca aaggtgtaaa
     7741 tcacaagatg gctgttgttc ccctcattgc aggcttcctg ttttttgcag tttcagaact
     7801 gacagcccag gaaaaaccat gtattgtcaa acatcctgcc ctttcggaaa aatattcagg
     7861 cgattgtaaa aacgggctgg caaacggaaa aggagctgcg caaggcattg acaaatacca
     7921 tggccagttc aggaatggcc tgccgcatgg caaaggcact tatatctggg ccgacggaac
     7981 ccactacacg gggcagttca aaaacggact caaagacggg aaaggcacac tggtaaccaa
     8041 agattctact gtaaccggtt actggcaaga ggacaggtat atcgggaaaa gaattgtcgc
     8101 gccgtaccag ataaca
//
LOCUS       DAOOYG010000120         8081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_9911_length_8081_cov_6.050461, whole genome shotgun sequence.
ACCESSION   DAOOYG010000120 DAOOYG010000000
VERSION     DAOOYG010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9911_length_8081_cov_6.050461"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1041)
                     /gene="rsmH"
                     /locus_tag="PK288_09425"
     CDS             complement(145..1041)
                     /gene="rsmH"
                     /locus_tag="PK288_09425"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPM19186.1"
                     /translation="MYYHVPALLDESIRGLNIRPDGIYVDATFGGGGHSMAILKRLRN
                     GRLIAFDQDEDAMRNVPANKNLIFLNQNFRFLKNNLRHLGIEAIDGLIADLGVSFHQF
                     DEPERGFTFRQDVTLDMRMNKKGNVTAASLLRTLDENAIAKLMYDYGELVNARKIARE
                     LVRARAVKPLTKVSDIISALGTMAPVRQENKFYARVFQALRIAVNHEIDYLKELLDQS
                     LEMLKKDGRLVVITYHSLEDRIVKNFMKTGNFEGEEKKDFYGNIITPFLTISKKGIVP
                     GERETEDNKRSRSARLRIAQKI"
     gene            complement(1041..1508)
                     /locus_tag="PK288_09430"
     CDS             complement(1041..1508)
                     /locus_tag="PK288_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mraZ"
                     /protein_id="HPM19187.1"
                     /translation="MATFIGDYTCKVDVKGRIILPAAFKKQMPAAAQDHFVVRKDIFE
                     NCLVLYSIDDWNRQLEKIRKKMNPYNREHNRFLRNFFKGTAELSLDNNNRMLVPKRFL
                     DLIGAERDVVLAGQDGRIEIWAASVYDTIDMPPDDFADLAEKLLGGTSNEPVE"
     gene            1754..2611
                     /locus_tag="PK288_09435"
     CDS             1754..2611
                     /locus_tag="PK288_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPM19188.1"
                     /translation="MDDHSVRNENLKIDVREVLLAKNPAVGKRIPGFIIKYLERIVHQ
                     DEINDFLAMYGHLHDSQFIAAGLRHFGITCNVYGRGNIPLSGRHIFVSNHPLGGLDGL
                     VFINELAGYYSDLKFPVNDILMNIRNLSGIFIPVNKHGAQGREAARMIEEAYASDSQI
                     LYFPAGLCSRKRNGVIRDLEWHKSFIQKAVQHKRNVVPAYFSGRNSDFFYNLSNVRNF
                     LGIKTNLEMLYLADEMFRQKDKVINLVFGKAIPWTRFDRSRTYPEWAGWVKDKVYELE
                     LFITSNDKK"
     gene            2679..3641
                     /locus_tag="PK288_09440"
     CDS             2679..3641
                     /locus_tag="PK288_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM19189.1"
                     /translation="MEPVADQLPREQIIAELTKDKLLRRTNNGNNEVYIFDNNNSPTL
                     MHEVGRVREITFRHAGGGTGKKLDIDEFDLDRKDPQKQLIVWDPERQMIIGGYRFYFP
                     EKGCVDCDITKLASSAYFRFSDKFLKKYYPHLMELGRSFVHPDYQSRTLAHKSLYALD
                     NLWDGLGALILDNHHLKFLFGKVTMYPHFNQKARNMILYFLKRHFGDPDKLVVPLEPA
                     DIDIKEDEMKKIFRGWRYKDNYKILSREVRNLNETIPPLINAYMNLSPTMRTFGTFIN
                     HKFGNVEETGIMITLRDIYIEKINRHLASYKKDFLITWFSHKDQ"
     gene            complement(3635..5536)
                     /locus_tag="PK288_09445"
     CDS             complement(3635..5536)
                     /locus_tag="PK288_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPM19190.1"
                     /translation="MSEEIRHECGIAMLRLRKPQEYYMKKYGAWDWGLQKMYLMMEKQ
                     HNRGQDGAGIAGIKMNVGPGNRYIFRQRSGKTNPIKEIFRLIYEDIDKIKITNGGNFT
                     DPEFIKSTVPFACDIYLGHLRYGTYGNYNIDHVHPVSRENNWKSRNLVMAGNFNLTNV
                     SEIFQFLVDLGQNPVDFSDTVTILENVGHRLDEENERIFRIFKEQGYSKREITPLIEK
                     HIDLATVLGKASRNWDGGYVMAGIVGHGDAFAIRDPAGIRPAFWFTDDEVAVVASERP
                     VIQTIFNVRTDRIRELPPASALIIKASGEFSVERIREETEPRKCSFERIYFSRGTDKS
                     IYRERKKLGELLTIPVLEKVDFDVENTVFSYIPNTAESAFYGLVKGVEDYINQSKIDA
                     ILAEPNNLSSEELARIISRRPRVEKIAVKDVKLRTFIAEDKTRKDLAGHVYDVTYGVI
                     RRGIDNLVVIDDSIVRGTTLQQSILSILDRLEPRKIVIVSSSPQLRYPDCYGIDMANL
                     EDLVAFRAAIELLRDNSMEDLIFRICREAMEENRKPVAEIRNLVREIYKPFRTSVISA
                     KIASMLRTSDIRSEIEIVFQSVEGLHEACPEHTGDWYFTGHYPTPGGNKVANQSFINY
                     VERRMEKAY"
     gene            complement(5539..6006)
                     /locus_tag="PK288_09450"
     CDS             complement(5539..6006)
                     /locus_tag="PK288_09450"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPM19191.1"
                     /translation="MTGKILFKLLLLLFPAGICLPQEAVSGEYIFDRDTHAGPGRLTI
                     IQNPGIDTLLNRHTLYNAKLKGMEGFRIQIYNSSNRNAREESGKIQADFISKFPRELV
                     SYASFAEPGWYMIRVGDFRTKTEATKYLLMVRKVFPDAYLVPDIINFPDLNKQ"
     gene            complement(6026..6775)
                     /locus_tag="PK288_09455"
     CDS             complement(6026..6775)
                     /locus_tag="PK288_09455"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPM19192.1"
                     /translation="MSGHSKWSTIKRKKGANDAKRGKIFTKIIKEIIIAAREGGGDPE
                     TNARLRLAIQNAKGANMPKDNIERAIKKAVGSDADNYMEISFEGYGPGGIAVFAECLT
                     DNNNRTVASVRSAFNKHGGKLGTNGSLSSLFDRKGVFLIRNEGFNREEVELELIDAGA
                     QDFESDEDTLTVTCAMEDFGSVNRKLGELGIEPEEAGLKRIPNNTRKLDLESAKKVMR
                     FIEGLEDDDDIQNVYHNLEITDELAEELGNS"
     gene            6938..>8081
                     /gene="miaB"
                     /locus_tag="PK288_09460"
     CDS             6938..>8081
                     /gene="miaB"
                     /locus_tag="PK288_09460"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPM19193.1"
                     /translation="MSKALYIETYGCQMNVSDSEVVASILSGHGYATTGNIDEADLVL
                     INTCSIRENAEARIWARLRDLRRLKRKNPGVLIGLIGCMAERLKEQVIEKERLVDMVI
                     GPDAYRSLPSLVAGAEAGHKAINVLLSREETYADISPVRMDKNGVTAYVSIMRGCNNM
                     CAYCVVPYVRGAERSRDPGSILNEIRELFDNGCREVTLLGQNVNSYSFVNNGEKTNFP
                     GLLEKAALTDPLMRIRFSTSHPRDISDELLYTISRYDNICRHIHLPVQSGSSRILALM
                     NRDYTREWYMDRIAAIKRIIPDCSVSTDLITGFCTETEEDHQATLSLMEWIEYDFAYM
                     FKYSERPGTRAARKYPDDVPDEIKARRLNEIISLQNRLSARSKRRDT"
BASE COUNT         2068 a         1904 c         1787 g         2322 t
ORIGIN      
        1 tgccagcagt acggccagca tgatgatgta ccagatattc cggagtatga tcacatccga
       61 aactattata ccgcttagca gttcctttat gaagctgcct gatgcctttt tacccgcatt
      121 gtttcctgtt tctgtggtca tgtctcagat cttctgcgcg atcctgagcc ttgcgctcct
      181 ggatcttttg ttgtcttcag tctctctttc accgggaaca atgccttttt tgcttatggt
      241 aaggaaggga gtgattatat ttccatagaa atcttttttc tcctcacctt caaaatttcc
      301 cgttttcata aaatttttaa caatcctgtc ttcgagcgaa tgataggtta ttaccacaag
      361 ccggccgtcc tttttaagca tctcgagcga ctggtcgagc aattccttca ggtaatctat
      421 ctcgtggtta accgctattc tgagcgcctg aaaaaccctg gcatagaatt tattttcctg
      481 ccgcaccggc gccatggtcc ccaatgctga tattatgtcg ctcaccttcg tcagcggttt
      541 tacagcccgt gcacgaacaa gttcccgcgc tattttcctg gcatttacaa gctccccgta
      601 atcgtacatc agttttgcaa tggcattctc gtcaagcgtt ctgagcaggg aggccgctgt
      661 cacattgccc ttcttgttca ttctcatgtc gagcgtaacg tcctgcctga aggtgaatcc
      721 cctttccggc tcgtcaaact gatgaaatga aacccccaga tctgctatca ggccatcgat
      781 tgcttctatc cctaaatgcc tgagattatt tttaagaaac ctgaaattct ggttaagaaa
      841 gatcaggttc ttattggctg gcacgttccg catcgcatcc tcatcctggt caaatgcaat
      901 cagcctgccg ttcctgagcc ttttaagaat tgccattgaa tggccgcccc cgccgaaagt
      961 ggcatcgaca taaataccgt cgggcctgat gttcaaaccc ctgatacttt catccagcaa
     1021 agcaggtacg tgataataca ttattccaca ggttcatttg aagttccacc taaaagtttc
     1081 tcagccaggt ctgcaaagtc atccgggggc atatcgattg tatcataaac agatgcagcc
     1141 catatttcaa tcctgccgtc ctggcctgca agaaccacat ccctttctgc accaataagg
     1201 tcgagaaacc ttttgggcac aagcatcctg ttgttattgt cgagcgacag ctcggcggtt
     1261 cccttgaaaa agttccttag gaacctgttg tgctcccggt tatagggatt catttttttc
     1321 ctgatctttt cgagctgcct gttccagtca tcaatcgaat aaagcacgag acagttttcg
     1381 aaaatatcct tcctgaccac gaaatgatcc tgtgcagctg caggcatctg cttcttgaat
     1441 gccgccggaa gaatgatcct tcccttgaca tccaccttgc acgtgtaatc acctatgaat
     1501 gtggccatac acttttcctg ttataaactg ctaaagtaag aaaaaattat ccactttact
     1561 ccactttgct ccacttattt taatattttg ttgataactt tagaaagaaa gcctctgaat
     1621 tacactgtta tcggcagggt gtaaaaactg acaaagacag ccgggtcagt taaagatcaa
     1681 agccttatgc aatgatacct tagtgatttt ttcttaaatt tggagtctgt attttaaacc
     1741 ggctgttttt catatggatg accattcagt taggaacgag aaccttaaaa tagatgtccg
     1801 ggaggtactg cttgctaaaa atccggctgt tggaaaaagg atcccggggt ttataattaa
     1861 atacctggaa aggatcgttc accaggatga gatcaatgat ttcctggcca tgtacggtca
     1921 tcttcatgat tcgcagttca ttgcagccgg attaaggcat ttcggtatta cctgcaatgt
     1981 atacggaagg ggcaacatac ctttgtcagg gaggcatatt ttcgtatcga atcatccgct
     2041 gggcgggctt gacggtctgg tttttatcaa tgagcttgcg ggatactaca gtgatcttaa
     2101 attcccggtc aacgatattc tcatgaatat ccggaacctg tcaggaatct ttatcccggt
     2161 aaacaagcac ggtgcacagg gcagggaagc ggccaggatg attgaggaag cttacgcgtc
     2221 cgacagtcag atcctgtatt ttccggcggg attatgttca aggaagcgga acggggtcat
     2281 cagggacctg gaatggcata aaagctttat acagaaggcg gtgcagcaca aaaggaatgt
     2341 tgtgccggcg tacttttcgg gcaggaactc cgatttcttc tataatcttt ccaatgtcag
     2401 gaatttcctt ggaattaaga ccaatctgga aatgctgtac ctggctgatg agatgttcag
     2461 gcagaaggat aaggtgataa atctcgtatt cggaaaagct attccctgga ctcgttttga
     2521 cagaagcagg acataccctg aatgggccgg atgggtgaag gataaggtct atgaactgga
     2581 attgtttatc acttctaatg ataaaaagta aaaaaaaaca taatccggat ttgtatttaa
     2641 gagaatattc tgatatttgg cgagtttaac aaaccacaat ggaaccagtt gcagatcaac
     2701 tgccaaggga acaaataatt gcagagctta ctaaggataa gctcctgagg cgtacaaaca
     2761 acggcaataa cgaagtatac attttcgaca acaataattc accaaccctg atgcatgagg
     2821 tgggaagggt aagggaaatc accttcaggc atgccggcgg gggaaccggg aaaaaacttg
     2881 acattgacga atttgatctt gacaggaagg atccccagaa acagctgatt gtttgggatc
     2941 ctgaaaggca gatgatcatc ggcggctatc gtttttactt tcccgaaaag gggtgcgttg
     3001 attgtgacat caccaagctt gcctcctcag catatttcag attctcagac aagttcctga
     3061 agaaatacta tccgcatctt atggaacttg gacgatcatt tgtccatcct gactaccagt
     3121 cgcggacact tgcccacaaa agcctttatg ccctcgacaa cctgtgggac ggactgggtg
     3181 cattgatcct cgataatcat catcttaaat tccttttcgg aaaagtgacg atgtatcctc
     3241 atttcaacca gaaagcaaga aacatgatac tttattttct gaaaaggcat tttggtgatc
     3301 ccgacaagct ggttgttccc ctggaacctg ccgacattga cataaaggag gatgaaatga
     3361 aaaagatctt caggggatgg agatacaagg acaactacaa gatactgtct agggaagtca
     3421 ggaatctgaa tgaaaccatt cccccgctca taaatgccta catgaattta tccccaacca
     3481 tgcgcacttt cgggactttc atcaaccaca aattcggcaa tgttgaagaa acgggcataa
     3541 tgatcaccct gcgggatatt tatatagaaa aaatcaacag gcatctggct tcgtacaaga
     3601 aggattttct tataacctgg ttctcgcaca aggatcagta ggctttttcc atccgtctct
     3661 ccacataatt tatgaatgac tggtttgcaa ccttattacc gcctggggta ggataatgtc
     3721 cggtaaaata ccagtcgccg gtatgttccg ggcatgcttc atgaagtcct tcgactgact
     3781 ggaacacaat ttctatttca gacctgatat cactggttct cagcatagaa gctatctttg
     3841 cggaaatcac tgatgtgcgg aatggtttgt aaatttccct gaccaggttc ctgatctcgg
     3901 cgacaggttt ccggttctct tccatggctt cccggcagat cctgaaaatc aaatcttcca
     3961 tgctgttatc cctgagcagt tctatggcgg ctctgaatgc aacaaggtct tcgagatttg
     4021 ccatatctat cccgtaacaa tcgggatatc tcaactgtgg agacgatgat acgattacga
     4081 ttttacgcgg ctccagcctg tcgaggatac tgaggatgct ctgctgcagt gtggttcccc
     4141 ttactattga atcgtcaata acgaccaggt tgtcaattcc tcttcgtata accccatagg
     4201 ttacatcgta tacatggcca gccagatctt tacgcgtttt atcctcggca atgaatgtac
     4261 gcagtttcac atccttgacg gcaatctttt caaccctggg ccggcggctg atgatcctgg
     4321 ccaactcctc agatgacagg ttatttggtt ctgccagtat cgcgtcaatc ttcgactggt
     4381 tgatataatc ctcaactccc tttaccagtc cgtagaaagc gctttctgca gtattgggga
     4441 tatatgagaa aaccgtgttc tcaacgtcaa aatcaacttt ctccaggacc gggattgtca
     4501 gcaattcacc gagttttttt ctttccctgt aaattgactt atccgttccc cgggaaaaat
     4561 aaatgcgttc aaaagagcat ttcctcggtt ctgtctcttc gcggatcctt tccacggaaa
     4621 attcacccga ggctttgatt atcagtgcgc ttgccggggg taattccctg atcctgtcag
     4681 tcctgacatt gaagatagtc tgaatcaccg gacgttcgga agccaccacg gcaacttcat
     4741 catcggtaaa ccagaatgcc ggcctgatgc ctgccggatc ccttattgca aatgcatcgc
     4801 cgtgccctac tattccggcc atgacatagc ctccgtccca gttcctgctg gcttttccca
     4861 gaacagttgc cagatcgatg tgtttctcaa tgagtggtgt gatttctctt tttgaatatc
     4921 cctgttcctt gaatatcctg aagatccttt cattttcctc gtcgagcctg tgccccacat
     4981 tttcaagaat ggtaacagta tcggaaaaat cgaccggatt ctggcctaga tcaacaagga
     5041 actggaatat ctcgcttaca ttggtaagat tgaaattacc cgccatgacc aggttccttg
     5101 acttccagtt gttttcacgg ctgacggggt gcacatggtc aatattgtaa ttgccatagg
     5161 taccatatct caggtgtcca aggtaaatat cacaggcaaa aggaacagtg ctttttataa
     5221 attccgggtc agtaaaattc cccccgtttg ttattttaat cttgtcaata tcctcataaa
     5281 taaggcggaa aatctcctta atcgggttcg tctttcctga tctttgcctg aaaatgtacc
     5341 ggtttcccgg accgacgttc attttgatac ctgctattcc tgctccgtcc tgtcccctgt
     5401 tgtgctgttt ctccatcatc aggtacattt tctgcaagcc ccagtcccag gcaccgtatt
     5461 tcttcatata atactcctgc ggcttacgca gtctgagcat tgcgatgccg cattcatgcc
     5521 tgatttcttc actcatgctt attgcttgtt cagatccggg aaattaatta tatctggcac
     5581 aaggtatgca tcgggaaata cctttcgcac catcagcaga tattttgtgg cttcggtttt
     5641 tgtcctgaaa tcaccaaccc tgatcatata ccagccgggt tccgcaaaag aggcatagga
     5701 aaccagttcc cggggaaact tgctgataaa atctgcctgg atcttgcctg attcttccct
     5761 tgcattccgg ttggaactgt tgtagatctg tatcctgaat ccttccatcc ctttcaattt
     5821 agcattgtaa agggtgtgtc tgttgagaag ggtgtcaatg ccgggattct gtattattgt
     5881 cagtcttcca gggcctgcat gcgtgtccct gtcaaagata tattctccgg atactgcttc
     5941 ctggggaagg caaatacctg ccggaaataa cagcagtaaa agcttaaaaa ggatttttcc
     6001 cgtcatttta tgttaatggg ttgggctatg aatttccgag ttcttcggca agttcatctg
     6061 ttatttccag gttatggtaa acgttctgga tatcgtcatc gtcctccagt ccctcaatga
     6121 agcgcattac cttttttgcc gattcaagat ccagcttcct ggtgttattt gggattcttt
     6181 tcagcccggc ttcttcgggc tcgattccca gttcaccaag tttcctgtta acacttccga
     6241 aatcttccat tgcacaggtg acggtaaggg tgtcttcgtc agactcaaaa tcctgggctc
     6301 ctgcatcaat gagttcaagc tccacctctt cacggttaaa gccctcgttc ctgatcagaa
     6361 ataccccctt acggtcaaaa agtgagctga gggagccgtt ggtgccaagt tttcctccat
     6421 gcttgttgaa agcagatctt acggaggcaa cggtcctgtt gttattgtct gtgaggcact
     6481 ctgcaaaaac agcgattccg cctggtccgt agccttcgaa agagatctcc atgtaattgt
     6541 cagcatcaga gcccacggcc ttttttatgg ctctttcgat attatcctta ggcatgttgg
     6601 cgcccttggc attctgaatg gccagtctca gtctcgcatt tgtttcggga tcacctcccc
     6661 cttctctggc tgcaatgatt atctccttga ttatcttggt gaagatcttt cctcttttgg
     6721 catcattcgc acctttcttt cgtttgatcg ttgaccattt actgtgacct gacatactct
     6781 cctggtttgc atttcttatg caaaattaaa tatttcttta atacttcgct cattgtatgt
     6841 acttttgcga agttattttg aaagcaattg atggtatggg aagactcatc cccgactggt
     6901 gaggcagttc cctgtctcca tccaaataag tctggaaatg tcaaaggcac tttatatcga
     6961 aacgtacgga tgtcagatga atgtttccga cagtgaggtt gttgcttcga ttctgtcagg
     7021 gcatggttat gcaaccaccg gtaacatcga tgaagctgac ctggtactga taaatacctg
     7081 ttcgatcagg gaaaatgcgg aggcaaggat ctgggcccgt ctaagggatc tcagacgcct
     7141 gaaaagaaaa aatccgggtg tacttatagg actgatagga tgcatggcag aaaggctgaa
     7201 agagcaggtt attgaaaagg aacggcttgt ggacatggtc attgggccgg atgcctatcg
     7261 ttccctgcct tcacttgttg ccggtgcgga agctggccat aaggcaatca atgtactttt
     7321 gtcgcgcgag gaaacatatg ctgacatatc gccggtaaga atggataaaa acggagttac
     7381 ggcatatgtt tcaattatga gaggctgcaa caacatgtgt gcctattgcg tggtgccata
     7441 tgtcagggga gccgaaagaa gccgtgatcc cggatcaatc ctgaatgaga tcagggagtt
     7501 gttcgacaat ggctgcaggg aggtgactct tctgggccag aatgtaaatt cctattcatt
     7561 cgtcaataac ggggaaaaaa caaactttcc cggacttctt gaaaaggctg ccctgacgga
     7621 tcccctgatg aggataagat tctcgacgtc ccatcccagg gatatatcgg atgagcttct
     7681 ttacacgatc tcaaggtatg acaatatctg caggcacatc caccttcccg tacaaagcgg
     7741 cagcagcagg atccttgccc tgatgaacag ggattatacc agggaatggt acatggacag
     7801 gatcgctgca ataaaaagaa taattcccga ctgttctgtt tcaacagatc tgataaccgg
     7861 gttttgcacc gaaaccgaag aagatcacca ggctactcta tcactcatgg aatggattga
     7921 atatgatttc gcctacatgt tcaaatacag tgaacgtccc gggactcggg ctgcaaggaa
     7981 atatccggat gatgtgccgg atgagatcaa ggcaaggcgt ctgaatgaaa taatatctct
     8041 tcagaacagg ctttcagcgc gatcgaagcg gagggatacg g
//
LOCUS       DAOOYG010000121         8022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10043_length_8022_cov_5.430275, whole genome shotgun sequence.
ACCESSION   DAOOYG010000121 DAOOYG010000000
VERSION     DAOOYG010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10043_length_8022_cov_5.430275"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..1033)
                     /locus_tag="PK288_09465"
     CDS             complement(164..1033)
                     /locus_tag="PK288_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19194.1"
                     /translation="MKLKFPTAFLLISVALLFSCKEKEKTSDMNIIYIHHSTGGVIWQ
                     GKKASLFTRAVRKVSTRLADNLGGQAMLPALFRKYNKENNRNYHITETAFPKAAPYGW
                     ENYPYDYYNIWVKNAGNEPYLEEPTLELLTKQYQVVVFKHCYPVSNIQPDKDSADINS
                     DYKSLGNYKLQYNALRDKLHEFPDTRFILWTGAALAKDAVGKDEAGRAREFFTWVKEE
                     WDLPGDNIHIWDFYELETEGGLYLRDEYATSSSDSHPNPEFASKAVGLIFNRIIDVIE
                     NNGMKTNLKGEKI"
     gene            complement(1103..1651)
                     /locus_tag="PK288_09470"
     CDS             complement(1103..1651)
                     /locus_tag="PK288_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPM19195.1"
                     /translation="MRITGGRYRGRRIVPARGLKARPTTDLARESLFNILGNTVDFPS
                     AVVLDLFSGTGSISFEFASRGAMVHLVEIDRLNFAGIKKVIGEIGFANIKPVHIDARA
                     YLKICTFRYDIVFADPPYNLKWLDKIPELVFGAEILKDNGIFILEHPKTVSFADSKWF
                     TDHRHYGGVNFSFFSPAAGRDQ"
     gene            complement(1642..2484)
                     /locus_tag="PK288_09475"
     CDS             complement(1642..2484)
                     /locus_tag="PK288_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HPM19196.1"
                     /translation="MPLFELYDETLDINSTENYRLAVQLSPDDLSLSILDTLRNKFIL
                     IRSYEPETGNRFDDTGMGEIISRDDFLLRRFKKIHIVTPSSKSILVPSPLYEDSHRDE
                     YFRFNFHPDEGHIVMANKIQEPDANLLFTVPQSRLELIQSLFPGSNPVHHLEPLLQAV
                     AGGRRSSYGNHVHVHLENEFFNMIVHDQNSLRFCNTFNYQSLPDIQYYVFYVIKRLNI
                     GQDEVIYFSGKTEKYERMVQDFSRYARNIRYAEPSGNFTFSYVFTEMLRSKFFNLFMV
                     LNCE"
     gene            2711..4132
                     /locus_tag="PK288_09480"
     CDS             2711..4132
                     /locus_tag="PK288_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM19197.1"
                     /translation="MREKVIFNNLCKFLGNDPTDDQSSVLGLFADYICNDDTGRIFLL
                     TGYAGTGKTSVINALVKTLSALRFKTVLLAPTGRAAKVLSSYTGQNAYTIHKKIYRQK
                     SLKDGMGSFVLDRNLQRNAWFIVDEASMISNSENEASIFGSGKLLDDLVEYVYSGIDC
                     RLILVGDTAQLPPVGSAVSPALDSRVLNAYGFGLVAAELKQVVRQSETSGVLVNATRI
                     RLQIENEELERPQIDTIGFDDIKRITGEDLIDEITAGYDSAGMDETIIVVNSNKLANK
                     YNQGIRNRIFFREEEIGPGDLVMVVRNNYFITDEDSGIEFIANGDIAEVRKIRKYEEM
                     YGFRFADMELYFRDYDLEIASKVILDVLNLDAPALPSDRNRELFHNILADYAHIKTRK
                     KQYEAVRSDKYFNALQIKFAYAVTCHKAQGGQWERVFIDQGMFNRTGVTPDYLKWFYT
                     ALTRSTDRVYLVNFSNEFFPLSE"
     gene            complement(4171..4587)
                     /locus_tag="PK288_09485"
     CDS             complement(4171..4587)
                     /locus_tag="PK288_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPM19198.1"
                     /translation="MKSYRKELWFETSTRRAFLNITGHVSKCLQESGIREGLVLVNAM
                     HITASVFINDDEPGLHSDFEVWLEKLAPEKPYSQYKHNGFEDNADAHIKRTLMGREVV
                     VAVTNGQLDFGPWEQIFYGEFDGKRRKRVLVKIIGE"
     gene            complement(4736..6469)
                     /locus_tag="PK288_09490"
     CDS             complement(4736..6469)
                     /locus_tag="PK288_09490"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPM19199.1"
                     /translation="MLPIDDIRKRAEKWLGNEFNEETRKEVRWMLDNDEKKLIDAFYQ
                     DLEFGTGGLRGIMGAGTNRMNIYTLGMATQGLSNYIIRECGDSPVKVAVAHDCRNNSR
                     YFAETAADIFSANGFEVYLFESLRPTPELSYAIRRFKCQSGVVITASHNPPEYNGYKA
                     YWNDGGQVVPPHDEGVIAEVRKIRSVSEIRFNGNRDLIRIIGKETDDSFLNELLKVRL
                     NPEIIARHSGLGIVYTSIHGTGGILVPPALKLFGFTNVRTVPEQDIADGNFPTVKSPN
                     PEEPAALAMAIQKANETGAELVLATDPDADRLGVAVRNDKGEFVLLNGNQTGTLLTWY
                     ILSQYREKNLYKGNEYIIKTIVTTDLLDRIAEKHDVKCYNVLTGFKYFAELIRNLEGR
                     EKYIGGGEESYGYLPGDYVRDKDAVGSCALVAEVAAWAKDKGMTLYSLLRKIYSDYGL
                     YREKLINIVRKGKEGADEIKAMMNGYRDTPPTVINNSRVVKIDDYELQMSANCITGEK
                     SRLGLVKSDVLQFFLEDGTKISVRPSGTEPKIKFYFSVHTGLASPDEYEKTCEILDRR
                     ISDVINDMGLA"
     gene            complement(6481..7347)
                     /gene="rfbD"
                     /locus_tag="PK288_09495"
     CDS             complement(6481..7347)
                     /gene="rfbD"
                     /locus_tag="PK288_09495"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPM19200.1"
                     /translation="MALILVTGASGQLGNEIRVLSKNYSGYEFLFTDLDELDITDESA
                     VNDFVGQSAPDWIINCAAYNFVDKAETEEEKAFLVNSIAVRNLAGALRNTDGRLIHVS
                     TDYVFDGAANTPYNENSGVNPLSVYARSKRAGEENALTHPYSMVIRTSWLYSSFGNNF
                     VKTILKHAAEKEFLKVVFDQVGTPTYAADLASAIMTIAARVIRNQKAFNPGIYHYSNE
                     GVCSWYDFAIEIVSLSGLDCRVIPIRSDEFPSKVKRPSYSVLDKSKIKENYDLEIPYW
                     KDSLRKCINLLR"
     gene            complement(7360..7908)
                     /gene="rfbC"
                     /locus_tag="PK288_09500"
     CDS             complement(7360..7908)
                     /gene="rfbC"
                     /locus_tag="PK288_09500"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPM19201.1"
                     /translation="MKIIETGFEGLFIIHPRIFTDSRGYFFESYRSDNFIEAGISFEP
                     VQDNESKSVRGVIRGLHYQLKPHDQAKLIRVVAGKIYDVAVDLRKNSLTFGKWYGIEL
                     DSESKTQFFIPRGFAHGFSVLSDIAIIQYKCDNIYNPLHERGIALNDPDLGIDWKLDD
                     LVPVISEKDMKHPLFRDADNNF"
BASE COUNT         2056 a         1893 c         1758 g         2315 t
ORIGIN      
        1 agtggacggg actcgaaccc gcgacccccg gcgtgacagg ccggtattct aaccgactga
       61 actaccgcac caattcaaaa ttgcgatgca aaattaacgg attttttgat tagtcaaaat
      121 ttaagcgagg aaaaaatatc cgcttgtgaa cttaccctgt gcatcagatc ttttcacctt
      181 tcagatttgt cttcatgccg ttgttctcga tcacatctat gatcctgttg aagatcaggc
      241 cgactgcttt tgaggcgaat tccgggtttg gatgcgagtc ggaggaagag gttgcatatt
      301 catccctgag atacaggcca ccctctgttt ccagttcata gaagtcccag atatggatgt
      361 tatcccctgg taaatcccat tcctccttca cccaggtgaa gaactccctg gccctcccgg
      421 cttcatcctt accgacagca tcttttgcaa gggccgctcc ggtccataaa atgaatctgg
      481 tatcgggaaa ttcatgaagc ttgtccctca gtgcattgta ctgaagcttg taatttccaa
      541 gggatttgta atccgaattg atatcggctg aatccttgtc cggctgtatg ttgcttaccg
      601 ggtaacaatg cttgaataca acgacctggt attgttttgt gagtagttcg agggtgggtt
      661 cctccaaata tggctcatta ccggcattct tgacccagat gttataataa tcgtagggat
      721 agttttccca tccgtaagga gcagccttgg gaaaagctgt ttccgtgata tgataattcc
      781 tgttattctc cttgttgtat ttcctgaata gtgcaggcag catagcctgt ccgccaaggt
      841 tatcggccag acgggtactg actttcctga cggcacgggt gaaaagggag gcttttttcc
      901 cctgccagat cacaccaccc gtactgtgat gtatatagat gatattcata tcagaggtct
      961 tttctttttc cttgcacgaa aaaagcaaag caacagagat taacaggaat gccgttggaa
     1021 atttcagttt catgatcgat ggttgttatt taatggttct atgttcagat taacaaatat
     1081 atccacaaaa aggttttgtt tgctactgat ccctgccggc agccggtgaa aaaaaactga
     1141 aattcactcc gccgtaatgc ctgtggtctg taaaccattt gctgtcagcg aagcttactg
     1201 ttttaggatg ttcaagaata aaaataccat tgtctttcag aatttcagcc ccaaaaacca
     1261 gttcagggat tttgtcaagc catttcaggt tgtagggagg gtcagcaaac actatgtcgt
     1321 acctgaaagt acaaattttc agatatgccc tggcatcaat atggacaggc tttatattgg
     1381 caaaacctat ttcaccgatg acttttttaa ttcctgcaaa attcagccgg tcaatctcta
     1441 ccaggtgcac catggctccc ctcgaagcaa attcaaaact gatgctgcct gtcccggaaa
     1501 acagatccag cacgacagct gacgggaagt ccaccgtgtt accgagaatg ttgaaaagac
     1561 tctcacgggc aagatccgtg gtcggcctcg ctttcaggcc gcgggccggg acaattctcc
     1621 ttcccctgta tctgcctccc gttattcgca atttaaaacc atgaacagat taaaaaactt
     1681 actccggagc atttcggtga aaacgtaact gaaggtaaag ttcccggaag gttccgcata
     1741 acggatgttc cttgcgtatc gggaaaaatc ctgtaccatt cgttcatatt tttcagtttt
     1801 tccactgaaa taaatgacct catcctgacc tatgttcaat ctttttatga cataaaacac
     1861 ataatactga atatcgggca gggactggta attaaaggta ttgcagaacc tcagcgaatt
     1921 ctggtcatgg actatcatgt tgaagaattc attctcaaga tgcacgtgga catgattgcc
     1981 gtaggatgat ctgcggcctc ctgcaacggc ctgcaggagc ggctccaggt gatgtacggg
     2041 gtttgagccc gggaacagcg actgaatgag ctccaaacgg ctttggggaa cggtgaacaa
     2101 cagattggca tcaggttcct gtatcttgtt tgccatcact atgtgcccct catcagggtg
     2161 gaaattgaac ctgaaatact catcccggtg tgaatcttca tataggggag aaggcaccag
     2221 tatggatttg gatgatggag tgacaatgtg gatcttttta aaacgccgta aaaggaaatc
     2281 atccctgctg attatttctc ccatcccggt atcatcaaac ctgtttccgg tttcaggctc
     2341 gtatgacctt ataagaatga atttatttct caatgtatct aaaatgctga gagaaagatc
     2401 atcagggctc agctgaacag ccagcctgta attctcggtt gaattaatgt caagcgtttc
     2461 gtcgtaaagt tcaaacagcg gcatttattt cagctttttt tgtcatgttc ccccagcgtt
     2521 gtccgggata ttgatcaatc cataatccat cgtgaaagga aaggatgaaa ccagcccggg
     2581 atttcatgct gtgcagtata aaatcaaaca gatccattca gtgaagaatg attttcaccc
     2641 ggaatgttta agtttacaaa caaaaataga aaaattatcc aagcacctga ggattgattt
     2701 caacattgtg atgcgggaaa aagtaatatt caacaacctg tgcaagttcc tcggcaatga
     2761 ccccacggat gaccagtcat ctgtcctcgg gctttttgcc gactatatct gcaacgacga
     2821 taccggcagg atatttctcc ttacgggcta tgcaggcaca ggcaaaacaa gcgttatcaa
     2881 cgctttggtt aaaaccctgt ccgcactccg gtttaaaacg gtccttcttg cacccaccgg
     2941 aagggctgca aaagtgcttt cttcatatac agggcagaat gcttatacga tccataaaaa
     3001 aatatacagg cagaaatcct taaaagacgg catgggaagc ttcgtccttg acaggaatct
     3061 gcaaaggaat gcctggttta tcgtggatga agcttcaatg atatccaatt ctgaaaatga
     3121 agcttcaatt ttcggaagcg gaaaactgct ggatgatctt gttgaatatg tgtattcggg
     3181 cattgactgc cggctgatcc tcgtgggcga cacggctcag ctgccgccgg tgggctcagc
     3241 ggtcagccct gctcttgaca gcagggtatt gaatgcctac ggcttcggcc tggtcgctgc
     3301 ggaacttaag caggttgtca gacaaagtga aacatcaggt gtacttgtga atgcaaccag
     3361 aatacgtctg cagatcgaaa acgaagagct tgagagaccg cagatagaca ccatcggatt
     3421 tgacgatatt aaaagaatta cgggcgagga tcttattgat gagattacgg caggatatga
     3481 ttcagcagga atggatgaaa caattatcgt ggtcaattcc aacaagctgg ccaacaaata
     3541 taaccagggc atacgaaaca ggatattctt cagggaagag gaaataggtc cgggtgactt
     3601 ggtgatggtt gtcaggaaca attactttat aacggatgaa gacagcggta ttgaattcat
     3661 tgccaacggc gacattgccg aggtcaggaa aataagaaaa tatgaggaaa tgtacggatt
     3721 ccgctttgcc gatatggaat tatatttcag ggattatgat cttgagattg catccaaggt
     3781 tattcttgac gttctgaacc tcgatgctcc ggcacttcct tccgaccgga acagggaact
     3841 ttttcacaac atacttgccg actacgccca tatcaaaacc cggaaaaaac aatacgaagc
     3901 cgtcaggagc gataagtatt ttaatgccct tcagataaag tttgcatatg cagttacctg
     3961 ccacaaggcc cagggagggc agtgggagag ggtctttatt gatcagggca tgttcaacag
     4021 aaccggggta acgcccgatt acctgaaatg gttctatact gcactgacaa gatccaccga
     4081 cagggtgtac ctggtcaatt tctcaaatga gttctttcct ctttctgagt agatttattg
     4141 tatgtgatcc gccattgaat tagtgacagt ctattctcct atgatcttga caagaacgcg
     4201 cttccggcgc tttccgtcaa actcgccgta aaatatctgt tcccaaggtc cgaaatccag
     4261 ctgcccgtta gtaacggcca caacaacttc ccttcccatc agggttcttt tgatatgagc
     4321 atcggcatta tcctcaaatc cattgtgttt gtactggctg tagggttttt ccggtgcaag
     4381 cttctcgagc cagacctcaa aatcgctgtg aagacccggt tcatcgtcgt tgatgaatac
     4441 gctggctgtt atgtgcattg cgttcaccag gaccagccct tcccttatac cgctctcctg
     4501 caggcatttt gaaacatgcc ccgtaatgtt taagaaagct cttctcgttg atgtttcaaa
     4561 ccagagttct tttctgtaag atttcatcct tatctgattt tattgttaat taattaacag
     4621 tagccggctg atgaccggtc tggaaatagt cctgtcactt ttctgcaaat taacctgatg
     4681 attatggaaa agcatttttt tcctgccgga taatcagtcc ggaaatgcat cgggatcagg
     4741 ccagtcccat atcatttatc acatcagata tgcgcctgtc gaggatctca caggtctttt
     4801 catattcgtc gggagatgca aggccggtgt gcacactgaa ataaaacttg atcttcggtt
     4861 cggtcccgga cggccgtaca gagatcttcg ttccgtcctc aaggaaaaac tgcaatacat
     4921 ctgatttcac caggcccagc cggctttttt cccctgtaat gcaattggct gacatctgca
     4981 gttcgtaatc atcgatcttt acaaccctgc tgttattgat gaccgtcgga ggagtgtccc
     5041 tgtaaccgtt catcatggct tttatctcat cggctccttc ctttcccttc ctgacaatgt
     5101 ttataagttt ttccctgtac agtccgtaat ctgaatagat cttacggaga agggaataaa
     5161 gcgtcatccc tttatccttc gcccatgccg caacttcagc aacaagtgca cacgagccga
     5221 ctgcatcctt gtcccttaca taatcgccgg gaagatagcc gtaactttct tcgccaccgc
     5281 caatgtattt ttctctgcct tccagattcc ttataagctc ggcaaaatat ttgaatccag
     5341 tgagtacgtt gtagcatttg acatcgtgct tttcagctat cctgtcgagc aggtcggtgg
     5401 taacgatcgt cttgataatg tattcattac ccttgtacag attcttttcc ctgtactgtg
     5461 aaagtatgta ccacgtcagc agtgtaccgg tctggttgcc gtttaaaagt acgaattcgc
     5521 ctttgtcgtt tcttacggcc acccccagcc tgtcggcatc gggatcagtg gccaggacaa
     5581 gctctgcccc ggtttcattg gccttttgta ttgccatggc cagggcagcc ggttcttccg
     5641 gattgggtga tttgacagtg gggaaatttc cgtcggcaat atcctgttct ggtaccgtcc
     5701 gtacattggt gaacccaaag agtttcagtg ctggcggtac cagtatgccg cccgtaccgt
     5761 gaatagaggt atatacaatc cccaaaccgg aatgccttgc aataatttca ggattcagcc
     5821 ttaccttgag cagctcgttg aggaaggaat catcggtttc tttcccgatt attcttatca
     5881 ggtccctgtt cccgttgaac cggatctccg acaccgacct gatcttcctt acttcagcga
     5941 tgacgccttc atcgtggggt gggaccacct gcccgccgtc gttccagtat gccttgtatc
     6001 cgttgtattc cggaggattg tgggatgcag taatgacaac cccgctctga catttgaagc
     6061 gtcttattgc atacgatagt tccggggtgg gtctgagtga ttcgaagaga tacacttcaa
     6121 atccgttggc tgagaaaatg tctgccgcag tttcggcaaa atacctgctg ttgtttctgc
     6181 agtcgtgcgc tacagccacc ttgaccggtg agtcaccgca ttccctgata atatagttag
     6241 acagcccctg ggtggccatg ccaagcgtat aaatgttcat cctgttggtt ccggcaccca
     6301 taatgcccct taatcccccg gttccaaatt ccagatcctg gtaaaatgca tcaattagct
     6361 ttttttcatc attatcgagc atccatctta cttccttgcg ggtttcttcg ttgaattcat
     6421 ttccgagcca tttttcggcc cttttcctta tgtcatcaat tggcagcata gcggtatggt
     6481 ttatcttagt aggttaatgc atttcctcag actgtctttc cagtatggta tttccaggtc
     6541 gtaattctcc tttatcttgc ttttatcaag aactgaatat gaaggcctct tcacttttga
     6601 agggaactca tccgacctga tgggaattac cctgcaatcc agtcctgata aagatactat
     6661 ttcaattgca aaatcgtacc agctgcatac cccttcattt gaataatgat atatgcccgg
     6721 attgaaggct ttctgattcc ttatcaccct ggcggcaatc gtcattatgg cggaagcaag
     6781 gtctgcagca taggtgggtg tgccaacctg atcaaacaca actttcagga attctttttc
     6841 ggcagcatgc tttaggatgg tcttgacgaa attattccca aacgacgagt aaagccatga
     6901 tgttctgatg accattgaat aaggatgtgt cagggcgttt tcctccccgg cgcgtttcga
     6961 cctggcatac actgacaggg gattgactcc tgaattttca ttgtacgggg tatttgcagc
     7021 cccgtcaaat acataatcgg ttgacacatg gatcagtctt ccgtcagtgt tccgcaatgc
     7081 tcctgcaagg ttcctgactg cgattgaatt tacgagaaag gccttttcct cttccgtttc
     7141 agctttgtca acgaaattgt aggcggcgca gttaatgatc cagtcgggag ccgattgtcc
     7201 gacgaaatca ttcaccgcac tttcatcagt tatgtccagt tcatccaggt ccgtgaaaag
     7261 aaattcatat cctgaataat tttttgaaag aaccctgatc tcgttcccga gctgacctga
     7321 ggctcccgtc acaagtatta aagccatccg ttatttcttt taaaagttat tgtcggcatc
     7381 cctgaacaga ggatgtttca tatccttttc acttattacc ggtaccaggt catcaagctt
     7441 ccagtcaatg ccaagatcag gatcattcag ggctattcct ctttcatgca gagggttata
     7501 aatattgtcg catttgtact gaattatggc tatatcgctg agtacggaaa aaccgtgggc
     7561 aaatcccctg ggaataaaaa actgtgtctt gctttccgaa tcaagttcga tcccgtacca
     7621 tttcccgaat gtaagggagt ttttcctgag atcgacagct acatcataaa tctttcctgc
     7681 aaccaccctg ataagcttcg cctgatcatg cggcttaagc tgataatgca gtccccttat
     7741 cactcccctc actgattttg attcattgtc ctggaccggt tcaaaagaaa tacctgcttc
     7801 aatgaagtta tcacttctgt aactttcgaa aaagtatccc ctgctgtcag taaaaatcct
     7861 gggatgtata ataaaaagtc cttcgaatcc tgtttcgatg attttcatct tattcctgtt
     7921 aactggctat tcaaaaacgt ttatttcttc tgccgcaatc ctgataagat actggccgta
     7981 ctggcttttc cttaccggtt cagccagtat cttatcagga tt
//
LOCUS       DAOOYG010000122         7936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10209_length_7936_cov_7.424058, whole genome shotgun sequence.
ACCESSION   DAOOYG010000122 DAOOYG010000000
VERSION     DAOOYG010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7936
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10209_length_7936_cov_7.424058"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PK288_09505"
     CDS             complement(<1..835)
                     /locus_tag="PK288_09505"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM19202.1"
                     /translation="MARILNRRKFIGTTSFTAAGVMMGSSFAAGCSPDVHRAVEPYDI
                     MADVMKYRKIDAHCHPEDDLAKQIETADRLGIEWLQISRPVTNFSGTEPEGPDVVRQH
                     NDEVYKAMKAYPGRFVGFFTLNPIYRKESLEEIKRCVDLGMSGYKGYIQVKVNDPLYY
                     PIIEKLIDLKMVVFMHTFCQMGMGGYRMKYDTGRFPGTTLPEDMVEAAKRYPEAMFHF
                     AHIGGGGDWEYECKMLKQCPNIFVDTGGSNNEEHMIDFAIEQLGEDRIFFGTDNCYHH
                     GI"
     gene            complement(891..1928)
                     /locus_tag="PK288_09510"
     CDS             complement(891..1928)
                     /locus_tag="PK288_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPM19203.1"
                     /translation="MSKKQFSRRDFVQKTALTAGMAPIASFSFPGTPLPQPGHGDSPP
                     RQVWIAGISQAGIRTKTPEEMVARVFGILADVKIHKPDFVCLPEVFPFEYVDKETTLK
                     EKIEISEKVLEQFSEFSRQNECYTICPVYTSSGGRVYNSAVVFDRSGKSIGQYNKIHE
                     TTGMIKYGISCGALFQPAIKTEFGPVGIQICYDINWDDGWKMLNEQGARIIFWCSAFD
                     GGMQLNMKALRYKCIVASSTNKNNAKVCDISGETLATTGIWNPNFYCGQVNLEKIFLP
                     TYDYLKEVREIEQKYGRKVRIKVFHEEEWTIIESLSPDIFIADIAKEFNLKSHDEGLR
                     EAEIIQDRSRG"
     gene            complement(2016..3308)
                     /locus_tag="PK288_09515"
     CDS             complement(2016..3308)
                     /locus_tag="PK288_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPM19204.1"
                     /translation="MKGKIPFGFDGIIVNSGKKSIISIKAPGNPALLLSALFFTLLFF
                     THGCTGHQSQIGRQWSFDIPPDYLREAPDIPPFWISSYDGVNEFLEKTVKKGQVEIIG
                     VSAGGRPIRAVFYGKQRQGKGTTTFSGSLGFGDVRAYRGHDHDKTVYMAMAAVHGGEF
                     EGIVGMVNLISVIETGKDLRGMEWPGITEVVSRLDRIVLIPVTNPDGRARIPLRMELY
                     RDTVFTVPEFLNTGGKPDGSITGWPQIKEFIPMDFSKPGFPGGYPNDAGVNFMHDDFL
                     GNIQPETRALFDLTEREKPDLIINLHTGAVYMRMDRPLGEPALRPVFDTLFRYVHSEL
                     ALRGLQRTNDPVIEGNPENASSGVYNLDGALNWHCGALSVVVESPSHAFSANSTTRER
                     QIESPDNLLNAQLICHQQSMKFLAETGGRSKWLPARRP"
     gene            3699..4093
                     /gene="ssrA"
                     /locus_tag="PK288_09520"
     tmRNA           3699..4093
                     /gene="ssrA"
                     /locus_tag="PK288_09520"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4180..4410)
                     /locus_tag="PK288_09525"
     CDS             complement(4180..4410)
                     /locus_tag="PK288_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19205.1"
                     /translation="MKNMKFITGAICGLITGLVLFMVISFRSDPAVFPAQSSGKITNL
                     NSRTEVFRLNVDNVQYIVVVSVDGGTAIVRHR"
     gene            4686..7190
                     /locus_tag="PK288_09530"
     CDS             4686..7190
                     /locus_tag="PK288_09530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPM19206.1"
                     /translation="MKRIYGIVFLLLTATVTWAQNRVLCSDWKISGPNQLYLPVFADE
                     RSVDGKKFDQAALMENILADKEGVDKWNTLNLVGDSLIPPVSGKNQLVQLAGFLKSDR
                     WTKASVSISTNALFELYLDGNRIKTQGMTSVKPVKIEMSLTTGVHGLLLKMLSTEKSM
                     KFSASVEAVNDSAKLFWTTNARRTLTIHDILEGEKMSNALLSASGKYLLIRTNTILPG
                     SGKSQFSFRVYDMESNRNLFALRNNALRADWLPKTDRLFYQVDNEGSSDIYLFDIKTG
                     EESLAASGLKSPRGIKWSPNEDYFLFSRDEEAEKPGDLKRVFNNDDRIPGTRSRSYLY
                     CFDMRTRMARPLTAGGESATLHDIKPDGTRILFSISRTDYSEVPFSKQNLYELELVSM
                     KPDTIWQNKLHAGYCQYSPDGTQLLVTGSSETFGDMGVKVSPERIPNSYDEQLFLFDI
                     GSKKAEALTRDFDPSVGSVYWTFDGKIYLTADERDYVNLYRFDLKKRNFTKINVPVEV
                     INSVNFAARKPVAVFEGSSISTPGRLYAIDLNSAKSTLLLDPGKEMLENVEFGQTQEW
                     NFINSRGTTIYGRIYYPPGYDPSKKYPVIVNYYGGTSPTNRAFGGRYPKNIWAAEGYM
                     VYVLQPSGSTGFGQDFSALHVNGWGREAIDDIIEGTKKFLEAFPSADAENVGCIGASY
                     GGFTTMTLQTRTDIFKTAISHAGISDISSYWGEGYWGYSYSSGATRYSYPWNRRDIYV
                     DNSPLFNADKFRNSILLLHGTDDTNVPVGESLQFYAALKILGKEVEMVLISGENHHIL
                     DYKKRLQWHDTIMAWFDYKLKNQPGHWNEMYPEKNL"
     gene            complement(7330..>7936)
                     /locus_tag="PK288_09535"
     CDS             complement(7330..>7936)
                     /locus_tag="PK288_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19207.1"
                     /translation="ALTSILSLGVHPFVTFFLGRSRMAVESLAVLPVVSSLVFIFRSM
                     GLSYQEVNIALIGKEKQNYRELRNFAIYLAFAVTVLISVLAFTPLADLWFTNISGLSM
                     ELAGLAYLPLKILILLPALTVLLNFQRSTQIINGTTGPISVATAVELIGIIIILLVCV
                     VFLDLIGVIAASVSFIVGRVLSNLYLVPKHASVVNGWKMGS"
BASE COUNT         2231 a         1908 c         1702 g         2095 t
ORIGIN      
        1 cgataccatg atggtaacag ttatccgtac cgaaaaagat ccggtcttct ccgagctgtt
       61 ctatggcaaa gtcgatcata tgttcttcat tgttgctgcc cccggtgtca acaaaaatat
      121 tcgggcactg tttaagcatc ttgcactcat attcccagtc gccgcccccc ccgatatggg
      181 caaaatgaaa cattgcttca ggatatcttt ttgctgcttc aaccatatcc tcgggaagtg
      241 ttgttcccgg aaaccgcccg gtatcgtatt tcatcctgta gccccccata cccatctggc
      301 agaaagtatg cataaaaacc accattttca ggtcgatcag tttttcgatt atgggataat
      361 acagcggatc attcactttc acctgtatat aacccttgta tccggacata ccgaggtcaa
      421 cacatctttt aatctcttca agcgattctt tccggtagat cggattcagg gtaaaaaaac
      481 ccacaaaacg tccgggataa gctttcatgg ctttatatac ttcatcgtta tgctgccgca
      541 caacatcagg accttcaggt tccgtgcctg aaaaattcgt tacaggccgg gagatctgaa
      601 gccactctat tcccagcctg tcggctgttt cgatctgctt tgccagatca tcttccggat
      661 ggcagtgagc atcgatcttc ctgtatttca ttacatcagc cataatatca tagggttcaa
      721 ctgcccgatg cacatccgga gaacagcctg ctgcaaagct gcttcccatc atcacgccag
      781 ctgcagtgaa ggaagttgtg ccgatgaact tcctccggtt taatattctc gccataatat
      841 attatctttc cttaacttac tataagtatc catatttcat tgccatcgta ttatccgcgc
      901 gaacgatcct ggataatctc agcctccctg aggccctcat cgtgtgattt cagattgaat
      961 tccttagcaa tatcagctat aaaaatatcg ggagacaaac tctcgattat cgtccattcc
     1021 tcctcatgaa agacttttat tcttactttc ctgccgtatt tctgttcaat ctccctgact
     1081 tccttcaggt agtcatatgt cggcaaaaaa atcttctcca gatttacctg accgcaatag
     1141 aagttaggat tccatatacc ggttgttgcc agagtctctc ccgaaatatc gcatactttt
     1201 gcgttgtttt tatttgtact ggaggccacg atgcatttat acctcaaagc tttcatatta
     1261 agttgcattc cgccgtcaaa tgccgaacac cagaaaataa tccttgctcc ctgctcatta
     1321 agcattttcc atccgtcatc ccagtttata tcataacaaa tctgaatgcc aaccggtccg
     1381 aactcagttt ttatggccgg ctggaacaat gcgccgcagg aaattccata tttaatcatt
     1441 cctgtcgttt catggatctt gttgtactgg ccaatacttt tcccgctcct gtcaaatacc
     1501 acggccgaat tgtaaaccct gccgcccgat gatgtataaa cagggcaaat agtgtaacac
     1561 tcattctgcc ttgagaactc tgaaaactgc tccaatacct tttctgaaat ctcaatcttc
     1621 tccttcaggg tggtctcctt atcgacatat tcaaatggga atacctccgg aagacatacg
     1681 aaatcaggtt tgtggatctt gacatcagca agtataccaa atacgcgtgc aaccatctcc
     1741 tcgggtgttt ttgtccttat cccggcttgt gagatgccgg cgatccatac ctgtcgcggt
     1801 gggctgtcac catgaccggg ctgtggaagg ggcgtgccag gaaaggaaaa agatgcaatt
     1861 ggagccattc ctgccgtaag tgcagttttt tgtacaaagt cacgtcggga aaattgcttt
     1921 ttactcatgg ctgagattta taacagggtt aacttaataa gaactctaac ttatgcattt
     1981 cgctgattcg ggcattcctt tctgatggaa tactatcagg gcctgcgggc cgggagccat
     2041 ttggaccttc cgcccgtttc ggcaaggaac ttcattgatt gctgatggca gatcagctgg
     2101 gcattgagca gattatcggg agactctatc tgtctttccc ttgttgtact gtttgcggaa
     2161 aatgcatgac tgggtgactc cacgactaca ctcaatgcac cgcaatgcca gttgagtgcc
     2221 ccgtcaagat tataaacccc ggatgaggca ttttcgggat ttccttcaat caccggatca
     2281 tttgtccgct gaagtcctct gagtgccagt tctgaatgga cgtatctgaa aagagtgtca
     2341 aagaccggcc ggagcgccgg ttctccgagc ggccggtcca tccgcatata aactgcgccg
     2401 gtatggagat ttatgatcag atccggcttc tcacgttcgg taagatcaaa cagtgcccgt
     2461 gtctccggct gtatattccc aagaaaatca tcatgcatga agttgacgcc tgcatcgttt
     2521 ggatagccgc cggggaaacc cggtttggaa aaatccattg gaataaactc ctttatctgc
     2581 ggccatccgg ttatcgagcc gtcgggcttc cccccggtgt taagaaactc cggaactgta
     2641 aatacggtat ccctgtaaag ctccatcctg agaggtattc ttgccctgcc gtcaggattt
     2701 gttacaggga taagcacaat acggtcaagc cttgaaacaa cctctgttat accaggccat
     2761 tccatgcccc tgagatcctt acccgtttca attactgaga tgaggttgac cattcccaca
     2821 attccctcaa actcaccccc gtgaacggcc gccatggcca tgtaaacagt cttgtcatga
     2881 tcatgacccc tgtaggcccg tacatcgccg aatcccaatg atcctgagaa tgtggttgta
     2941 cctttgccct gacgctgttt tccataaaaa accgccctga ttggcctgcc tccggcggat
     3001 acgcctatga tttcaacctg ccccttcttt actgtttttt caagaaattc attgaccccg
     3061 tcatagctcg aaatccagaa tggcggaata tccggggctt cccgaaggta atccggagga
     3121 atgtcaaatg accattgcct gccgatctgg gattgatgac cggtacaacc gtgagtaaaa
     3181 aataataatg taaaaaataa agcagacagt aacaatgctg gatttccagg tgctttaatt
     3241 gatatgattg attttttacc ggaattcacg atgattccgt caaagccaaa ggggattttt
     3301 cctttcattt tgcatcaatt atatgggtca taaaaattaa acaaaacagc attctgcctt
     3361 agtctgctat atatcaacat attgatttct tcagccatgg taaccacagt atgaatttaa
     3421 ttaattttca tcaataaacc agagatgcgt gttgccgtaa gttttacctg caaaatggta
     3481 cccggtgtcc ggtattcggt attcgctgtc cggtgttcgg tattcagtgt ttggtgtttg
     3541 gttggtaact aaactgcgtc aaaaacataa cccaaagctc taaaccttaa acccgaaact
     3601 cgaaacccga aactcgaaac ccgaaacccg aaacactgaa tactgaatac cgcttgcata
     3661 aaaggcacgg aatttgtaat tttggccggt aataccacgg ggctgacctg gttttgacag
     3721 catgtggggt ggtatgcaag catgcaggat gtggtgacac gttccttgaa cagtgttaca
     3781 aaacaataac tggcgaatac aattacgctc tcgctgccta agcgaataaa gtagcttaag
     3841 cttcattccg gccaggttgc cgggacggga cgtttcccgg gcggtcatgc cctgatccaa
     3901 cccgatacgg agacgcacgc aattcagggc tggcccgacg attgttccgg tccgacggtg
     3961 agaaaaacag gaaataaggg catattaggt cgttctgcag ctgatgtgcc tcgaaaatca
     4021 agcaggacta agcatgtaga aaacatatgg tctccatgcc tggacgcggg ttcgactccc
     4081 gccagctcca cgttactcca cattaattaa gtgtggagtt ttctttttat aatataacct
     4141 gttacatgca acaacagcag tatattaata tgtcattgac tacctgtgtc tgacaatggc
     4201 agtcccacca tcaacgctga cgacaacaat atactgaaca ttgtccacat ttaaccggaa
     4261 tacctcggtt ctggaattga gattggttat ctttcctgat gactgggcag ggaaaactgc
     4321 cggatctgat ctgaacgaaa tgaccatgaa taaaaccaaa cccgtaatta atccgcatat
     4381 cgctcccgta atgaatttca tgtttttcat tttgtctgat agtttataat taatctgaat
     4441 gaaggctgtg cagcttaaat gtaaccgata tttaaatcat ctgcaactat attatgtttg
     4501 ccgcaattgc cctgagtcct gagtatatct tcccgaagat acaatttccc gtaatgaagc
     4561 tcccggcatt atcctcccga cataataatt gaaaatcagg atcgattctt tgttgccgga
     4621 aagcctgttt ttaatatatt tgaagcctgc aaacaatatt ctcactgaaa aatcaaacaa
     4681 gttatatgaa aagaatttac ggcatcgtgt ttctgctgct gacagcaacc gtaacatggg
     4741 cgcagaaccg ggtattatgc tccgattgga agatttcggg accgaaccag ctgtatttgc
     4801 cggtttttgc ggatgaacga tctgttgatg gtaaaaaatt cgatcaggcc gctttgatgg
     4861 aaaacatact ggcagataag gaaggagtgg acaaatggaa tactctgaat cttgtaggcg
     4921 acagtctgat accgcctgtc agcggaaaaa accaactggt tcagctagcc ggttttctga
     4981 aaagcgaccg ctggacaaaa gcctctgtga gtatttcaac caatgccctt ttcgagctgt
     5041 atctcgacgg caacaggata aagacccagg gcatgacatc tgtaaagcct gtgaagatag
     5101 aaatgagcct gacaacaggt gttcacgggt tgttgcttaa aatgctaagt acagaaaaat
     5161 cgatgaagtt ttctgcaagc gttgaagcag taaatgattc ggcaaagctg ttctggacca
     5221 ccaatgccag acgtactctt accattcacg acattctcga aggtgaaaag atgtcaaatg
     5281 cattgttatc agcttcaggg aaatacctgc tgatccggac aaatactatt ctccctggca
     5341 gtggaaaatc acagttttca ttcagggtat acgacatgga atcgaaccgg aatctgtttg
     5401 cactgcgaaa caatgcctta cgtgccgact ggcttccaaa aaccgatcgt ctgttttacc
     5461 aggttgataa tgaaggaagt agtgatatat atttatttga tataaagaca ggcgaagaaa
     5521 gtctggcagc ttcgggcctg aagtcacccc gtggaataaa atggtcacct aatgaagatt
     5581 acttcctgtt ctcacgtgat gaagaggctg aaaaaccagg cgacctgaaa cgtgttttca
     5641 acaatgatga tcgaattcca ggtacccgca gccgctcata tttatattgc ttcgatatga
     5701 ggaccagaat ggcacggcca ttaacagccg gcggtgaatc tgccaccctt catgatatca
     5761 aacctgacgg cacaaggatc ctgttctcaa tctcacggac cgattacagc gaagtaccct
     5821 tttcgaaaca aaacctttac gaactcgaac tcgtatcaat gaaacccgat accatctggc
     5881 aaaacaaact ccatgccggg tattgccaat attcccctga cggaacacag ctcctggtaa
     5941 caggaagctc agaaaccttc ggcgacatgg gtgtaaaggt aagcccggaa aggatcccta
     6001 acagctatga cgaacagctt tttctcttcg acatcgggtc aaaaaaagcc gaggcgctga
     6061 cacgcgattt tgatccgtct gtcggaagcg tttattggac cttcgatgga aaaatttacc
     6121 ttacagccga tgaacgtgac tatgttaacc tttaccggtt cgatcttaag aaacgtaatt
     6181 ttacaaagat caatgttcca gttgaggtca tcaattcagt taattttgca gcacgaaaac
     6241 cggtggccgt ttttgaagga agcagtatca gtacccccgg aagactgtat gccatcgatc
     6301 tgaacagcgc aaaatcaaca ctgcttctcg atccaggaaa agaaatgctg gaaaatgttg
     6361 aattcggaca aacgcaggag tggaatttta ttaacagcag ggggacaact atctacggga
     6421 ggatttacta tccaccgggt tacgatccct caaaaaagta tccagtgatc gtgaattact
     6481 acggcggaac ctcccctacc aaccgggcgt tcggaggccg ttatcccaaa aacatctggg
     6541 cggcggaagg ttatatggta tatgtattac aacccagcgg atcaaccggc ttcggtcagg
     6601 atttctcagc ccttcatgtt aacggatggg ggcgcgaagc aatcgacgat attatagaag
     6661 gtacaaaaaa gttcctggaa gcatttccct ccgccgatgc cgaaaacgtg ggttgcattg
     6721 gcgcttcata cggagggttc accaccatga cactgcaaac acgaaccgat atttttaaaa
     6781 ccgctatctc ccatgcagga ataagcgata tctccagcta ctggggggaa ggctactggg
     6841 gatattcgta cagctcaggt gcaaccaggt acagctatcc atggaaccga agggatatct
     6901 acgttgataa cagcccgctt ttcaatgccg acaaattccg gaattcaatt ttactgttgc
     6961 atggcaccga cgatacaaat gtaccggttg gcgaaagcct gcagttttat gcagccctga
     7021 aaatactggg aaaagaagtg gaaatggttc tgatcagcgg tgagaaccat catatactcg
     7081 attacaaaaa gcgtcttcaa tggcacgata ccattatggc atggtttgat tataaactta
     7141 aaaaccagcc aggacactgg aacgaaatgt atcccgaaaa aaatctgtag gaaaaaagac
     7201 ccaccactgg cttattcttc atgagaagga cctggtaaga ttccatagaa aaacggaagt
     7261 ttaaggtcag caacgcctga tttcctccaa tttgaacatt tactattgaa tctgcccggc
     7321 aaggcaaaat caggatccca ttttccagcc atttactact gacgcatgct tcggtaccag
     7381 gtaaagattc gacaatactc tgccgacaat gaaagataca gaggcagcaa tcacgcctat
     7441 caggtcaagg aacactacac acacaagcaa tattataatt ataccgataa gctctactgc
     7501 tgttgcaacc gagatcggac ccgtagttcc gttaattatt tgggtggatc tttggaagtt
     7561 cagcaagaca gttagtgccg gcagcagtat caggatcttc agaggaagat atgccaggcc
     7621 ggctagttcc atactcagcc ccgaaatatt tgtgaaccac aggtcagcca aaggagtgaa
     7681 ggcaagtaca gatatcagca ccgttaccgc aaatgcaaga taaatcgcaa aattcctcaa
     7741 ttcccggtag ttttgttttt ccttccctat cagtgcaata ttcacctcct ggtacgacaa
     7801 acccatactc ctgaaaataa ataccagtga actcacgacc ggaagaacag ccagtgactc
     7861 aaccgccatt cggcttcttc ccaggaaaaa agttacgaaa ggatgcaccc cgagcgaaag
     7921 tattgaagtc agcgca
//
LOCUS       DAOOYG010000123         7910 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10260_length_7910_cov_5.394653, whole genome shotgun sequence.
ACCESSION   DAOOYG010000123 DAOOYG010000000
VERSION     DAOOYG010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10260_length_7910_cov_5.394653"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1233)
                     /gene="dcuC"
                     /locus_tag="PK288_09540"
     CDS             complement(7..1233)
                     /gene="dcuC"
                     /locus_tag="PK288_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HPM19208.1"
                     /translation="MEVIGFIICIGVIAITAILLYRRFNPQGVLIIAGLLMVSLAALM
                     GLNPIAVNKPSGSVIFDLVQVIDDGFVSNVTRIGLMIMTIGGYVAFMNKIKATDSLVY
                     IALKPLAFFRKYPYLASVMAIPIGQVLFMTIPSAAGLGLLLVASFYPVLVSLGCSRMT
                     ALSVIAAATIFDQGPGSANTAMAASLIGIPNVEYFVTYQLPIVVPTTVIVMILYYFSN
                     RHFDRKEMKEGRDIFVKTSEKTVKPDVPLIYAILPMLPLILLVAFSEYVGLFDIHLST
                     TVAMLFSLIVSVVFMLLFKRNFRDVIDMLDSFWKGMGTVFSSVVTLIVSAEIFSKGLI
                     SMGFIDTLVSGSTHLGFSGAAISILITVLIFMAAMLMGSGNAAFFSFGPLMPDIAPSL
                     GMRRRLHCHCPLAWRP"
     gene            complement(1240..3021)
                     /locus_tag="PK288_09545"
     CDS             complement(1240..3021)
                     /locus_tag="PK288_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM19209.1"
                     /translation="MKKIKYLIIIVSVMIFASCSEDFLTEKPVDDIFAENLLVNHDGF
                     LNMQNAMFGLVRDEYDRGDRFYGGTSFGSIPFTKHTLFSAGADNSWGNNRHTNFRHLS
                     FPKNITDMEDVIPFQAVFEWLYKVINTSNMIISRAEYEGIDWQGSTAEEDLANKNTVI
                     ANARLVRAWAYRHLTYSFGAVPLSTEEITGSNYRTDWERTPVADIREVMEQDLLYAIA
                     ILPMRTATNTVPSQAVARHYLGELYLAMGRPEDAKAVLQPLVEGSEYTLMTARFGKNA
                     ANPGHAFIDLFRSPLYSDGNLEVLWAFPNIEPEKAASGYSPNSFMRNMWRNYYSSLSD
                     ISKLNHPDYPGGTIKLFWSLNGGKGAGRLAISLGAFNLYKYKDQDAVDVRYDEHSLVW
                     HLYFLNSDNQVYEVLKNGASLINLKPNAAMSNDTDPTIKQYNLPSTKKWDYVNPIFEQ
                     ADADNAYNDIVYLRLAETYLLYSEALFKIGEGGEFWLNKVRERAQVIPIEASEMSIDM
                     ILDERSRELITEEHRRHTLIRLSQENGGDERAASNIFKTRVRTYNEVSGREVRGMWDD
                     LTPVLLPIPQAFINSNSGRVISQNPGY"
     gene            complement(3042..5966)
                     /locus_tag="PK288_09550"
     CDS             complement(3042..5966)
                     /locus_tag="PK288_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM19210.1"
                     /translation="MKKTIILFLLLFPGLLATAQIRGKVTDGEKNEPFAGVTVQVAGT
                     QRGTFTDAAGMFSITARTGEMLLFSFIGYKPVEYNVTDEREINIVLFPDEMVLDEVIV
                     VGYGVQKRSDVTGSVASVSPETLESRPSANLLQSLQGSVAGLSISITGSNAEGSATTT
                     RIRGNRSITASNAPLVILDGVPYSGNWAEINPNDIASMEILKDASSSAIYGARGANGV
                     ILIQTKKGKDGEKVQLSYDASVTSSTAINIPKMMDGPTFGAMKLDAGLVHSFTEAEMY
                     AQNATTDWVDLALRNGFNQQHNLSLRGSGNKSRYFISGNYTMNKGIAKNDDFNRITLR
                     INFDQTVNKYVTIGTSTQYGFYNRSGNAADFDDAFTMNPLGRAYTESGELRLSTWEDA
                     SYARNPLSSLNDINVNHKNVLVSNNYIDINLPMKGLTYKLNTGYTYQTQAEQNYQGRN
                     TYEGLQNNGVLSISNNYQIDWLAENILSYNRLFGKHNIFLTGLYSAQGYKKESNGINA
                     TGFPSDVMTYYQPDKASFMNPRASQDKSNHISQMARANYSFDSRYLFTATVRRDGFSA
                     FGADRKYGVFPSIALGWNINNEQFFKSSGAGKVVNNLKLRLSWGKNGNEAISPYSSLP
                     NLRSLDYLSSDHTPLFGFYPSRLASALLGWETTTSNNLGLDFGILNDRINGSFDLYFT
                     KTYDLLLSRSIPPINGASEILENIGQTKGSGIDLQISSINIDRGKFRWITNLSFIHNR
                     SVLVDVGLYDENGNPIDDLASEWFIGHEVSVNYDYLFGGIWQEGESTEVDEAPGFIKY
                     IDVNKDGDITPEEDKQIIGRRVPLFTAGLNNTFIYGNLSLSVFINGQVGETRPNYLWS
                     PATLSYRENQLDKVFWTPENKLQTYPANKPDGSVNVRRMNFYEKTDFLRVQDITLAYS
                     FNRDLIAKAGLSRLEAYASVRNLLTLTSWSGLDPEFVSRGSVQRAIPQTRQLTFGIRT
                     SF"
     gene            complement(5992..7557)
                     /locus_tag="PK288_09555"
     CDS             complement(5992..7557)
                     /locus_tag="PK288_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HPM19211.1"
                     /translation="MDPRQLRTHKNQVILILAFFFCTMLSGQQSGLITQRYFKDPDVK
                     FDTPTLSLDENRFATYEEILNWIESNIAVHVNASVRIIGTTPLGQDIPVIYLSNGSSA
                     PKLRVWFQGLLHGNEPAGGEALLFLAKEILTTAEGENILDNLDIAILPIANIDGYIAN
                     TRRSSNGYDLNRDQTKFADPVSRIIKAAFISWQADLAFDFHEFQPTRREYAGIGEDGA
                     SISYDVLFLPSGYLNIPSGLRKASVDIFQKEAEAALERNGYTHNFYFTAGDYGDSIML
                     NKGAQSPHSSSTSYALSNAVSLLVETRGIGLGRTSFARRTHSARVVAGEFLLSALRHK
                     EELTTIINEARNETLSGKTDVVVTSTPSVCYRDVDFIDLSDGMLLKLRMRVNDAENLN
                     PEIVRPRPKGYIIEPGHEKEIEVLKLLGLEITQLRKGKKLKVQSYLIEKYEESETEWE
                     GIRTASVSARLISQRLPFAKGSYYVDLRQKNANYVISVMEPEAVNGFVSYRVTTTFQG
                     DTLPWHRIEERNR"
     gene            complement(7560..>7910)
                     /locus_tag="PK288_09560"
     CDS             complement(7560..>7910)
                     /locus_tag="PK288_09560"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HPM19212.1"
                     /translation="MVTAIQALGLNSVFVTLLLTLISFSASLITGSGVASFTTIGQLI
                     PDLALKFGIPSITFMIPVQLAAGLGRAASPIAIVIIAVSEIAGVSPFDIAKRNAGPLL
                     SISLLLVLLSYILL"
BASE COUNT         2106 a         1850 c         1750 g         2204 t
ORIGIN      
        1 tgatctttat ggccgccatg ctaatgggca gtggcaatgc agccttcttc tcattcccag
       61 acttggtgca atatccggca taaggggacc gaatgaaaag aaggctgcat tgccactgcc
      121 cattagcatg gcggccataa agatcagtac cgtaatcaga attgatatgg ctgcgcctga
      181 aaagcccagg tgagttgatc ctgaaaccaa tgtatctatg aagcccatgc ttatgaggcc
      241 tttggaaaaa atttctgctg aaacaatcag agtgactacc gagctgaaca ctgtacccat
      301 acccttccag aaactgtcaa gcatgtctat gacatcccgg aaattacgct tgaaaagaag
      361 cataaatact acagacacta tcagggaaaa aagcatggca acggtagtgc tgagatgaat
      421 atcaaacagc cctacatatt ctgaaaaagc aaccagaaga atcagtggaa gcatcggaag
      481 gatggcatat atcagtggaa catcgggttt cactgttttc tcagaagttt ttacaaaaat
      541 atcccggcct tccttcatct ccttcctgtc aaagtgcctg ttgctgaaat agtaaagaat
      601 catcactatg acagtggtcg gcaccactat cggaagttga taagtaacaa agtattcgac
      661 attgggaatc ccgataagcg aagctgccat tgctgtatta gcagaacccg gtccctggtc
      721 aaaaatcgtt gctgctgcaa tgacagagag ggcagtcatc ctgctgcacc ccaggctcac
      781 cagaacaggg taaaaggagg ctacaagaag aagtccgaga ccagctgcag atggaattgt
      841 catgaagagc acctgcccga tggggatagc catcactgaa gccaggtaag ggtatttcct
      901 gaaaaaggcc agtggcttta atgcaatgta gacgagggag tccgtggcct ttatcttatt
      961 cataaaggcc acatatcctc cgattgtcat gatcattaac ccaatcctgg ttacattgct
     1021 tacaaacccg tcatcaatca cctgcaccag gtcaaatatg acactgccgg aaggcttgtt
     1081 tactgcaata ggattgagac ccatcaaagc tgcaagactg accatgagca atcctgcaat
     1141 aataaggaca ccctgcgggt taaatctcct gtagaggaga attgccgtaa tggcaattac
     1201 tccaatacag ataatgaatc cgataacctc catgtttctc tagtatcccg gattctgaga
     1261 aataaccctg cctgaatttg aattgataaa cgcctgtggt attggtaaaa gaacaggtgt
     1321 cagatcatcc cacattcccc tgacttccct gccagagact tcattgtagg tccgtaccct
     1381 ggtcttgaag atatttgatg cagcccgctc atcaccgcca ttctcctggc tgagcctgat
     1441 aagagtgtgt cgtctgtgct cctcggtaat cagttcacgg cttctttcat caagtatcat
     1501 gtcaatgctc atctctgagg cttcgatcgg gattacctga gcgcgctccc tgaccttgtt
     1561 cagccagaat tcacctccct ctccaatttt gaagagagcc tcggaataaa ggagataagt
     1621 ctctgccaat ctcaggtaaa cgatatcatt ataagcattg tctgcatcag cctgctcaaa
     1681 tataggattt acatagtccc atttcttcgt tgaaggcagg ttgtattgct tgattgtagg
     1741 atctgtatca ttgctcatgg cggcgtttgg cttgagatta atcaggcttg ccccgttctt
     1801 gagaacctca tagacctggt tatcggaatt taaaaagtac agatgccaaa ccagtgaatg
     1861 ttcgtcatac ctgacatcta ccgcatcctg atccttgtac ttataaaggt tgaaagcccc
     1921 aagcgaaata gccagtcttc cggcaccttt tccgccgtta agcgaccaga aaagcttaat
     1981 ggtcccaccg ggataatcgg gatgattaag tttactgata tcggaaaggc ttgagtagta
     2041 gtttctccac atattcctca tgaagctgtt gggggaatac cctgaagctg ccttttcagg
     2101 ttcgatattg gggaatgccc acagaacttc aaggttgcca tcagaataca atggtgagcg
     2161 gaagagatca atgaatgcat gccccgggtt agctgcattt ttgccgaatc tcgcagtcat
     2221 gagagtatat tccgaacctt caaccagtgg ttgtagtact gcttttgcat cttccggtct
     2281 gcccattgcc aggtataact caccgagata atgccttgcc actgcctgcg atggtacagt
     2341 gttggtggcc gttctcatcg gcagaatcgc aatggcatat aaaagatcct gttccattac
     2401 ttcacgtata tcagcaacag gagtcctctc ccagtcagtg cggtaattgc tgcccgtgat
     2461 ctcttctgta gagaggggga cggcaccaaa cgagtaggta agatgcctgt aagcccatgc
     2521 tcttaccagc ctggcattgg ctataaccgt atttttgttt gcaagatcct cttccgcagt
     2581 acttccctgc cagtcaatac cttcgtattc tgccctggaa atgatcatgt tggaggtgtt
     2641 aatcacttta taaagccatt caaaaacagc ctggaaggga ataacatcct ccatgtcagt
     2701 tatatttttc gggaaactga gatgtctgaa attggtatga cggttatttc cccatgagtt
     2761 atcggctcct gcgctgaaaa gagtgtgttt ggtaaacgga atgcttccga acgaggtgcc
     2821 accataaaat ctgtccccac gatcatattc atctctgacc agtccgaaca ttgcattttg
     2881 catgttcaga aaaccatcgt gattgacaag cagattctcg gcaaagatat catctacagg
     2941 cttctctgtc aggaaatctt cgctgcagga agcgaaaatc atgacagaca ctataattat
     3001 taaatatttg atctttttca ttttaatagc actttttata attaaaaact ggtcctgatt
     3061 ccgaatgtta attgtcttgt ctgcggaata gctcgctgaa cagagcctct ggatacaaac
     3121 tccggatcaa ggcccgacca gcttgtgagc gtcagaagat ttcttacact ggcataggct
     3181 tcgagcctcg agagacctgc ttttgcaatc aggtctctgt tgaaagagta cgccagggtt
     3241 atatcctgta ctctgagaaa atctgttttt tcatagaagt tcatcctcct gacattgacg
     3301 gaaccgtcag gcttattggc cgggtaggtc tgaagcttgt tttccggggt ccagaaaact
     3361 ttgtccagct gattttccct gtaggacaga gtggcaggag accagagata gtttggtctt
     3421 gtctcgccaa cctgaccgtt tataaaaact gaaagagaga gattcccata gatgaaagtg
     3481 ttgttaagac ctgcagtaaa cagcggaacc cttcgtccga tgatctgctt atcttcctca
     3541 ggagtgatat cgccatcttt gttgacatct atgtacttaa tgaaccccgg ggcttcatca
     3601 acttcggttg attcaccctc ctgccatatt ccgccgaaca gataatcata gtttacgctc
     3661 acctcatgac caataaacca ctcggaggcg aggtcatcaa taggattacc gttttcatcg
     3721 taaagaccta catcaacaag gacgctcctg ttgtgtatga agctcagatt tgttatccac
     3781 cggaatttgc cacggtcaat gttgattgaa gatatctgta gatcaatgcc gcttcccttt
     3841 gtctgtccaa tattttcaag tatctcgctg gcaccattga tcgggggaat ggatctggag
     3901 agcaacaggt cataggtttt ggtgaaatag agatcaaatg aaccgttgat cctgtcattc
     3961 agtataccaa agtccagacc gaggttgttc gaagtggttg tctcccatcc cagaagtgca
     4021 gaagccagtc tcgaaggata gaatccaaac aacggagtgt gatcgcttga cagatagtca
     4081 aggcttctca ggttgggaag gctggaatat ggtgagatgg cctcatttcc attctttccc
     4141 catgacagcc ttaatttcag gttatttacc actttacctg ctccggatga tttgaaaaat
     4201 tgctcattat taatattcca ccctaatgca attgatggaa agactccata tttccggtca
     4261 gctccgaatg cggaaaatcc gtctcttctg acagttgcgg taaaaagata ccggctgtca
     4321 aaactgtagt ttgccctagc catctgggaa atatgatttg atttgtcctg ggaagccctg
     4381 ggattcataa aactggcttt atcaggctgg taataggtca taacgtcact cgggaatcct
     4441 gtcgcgttaa tcccgttcga ctccttttta taaccttgtg cggaatacag acccgtaaga
     4501 aagatattgt gcttgccgaa caacctgtta taggagagta tgttttctgc cagccagtca
     4561 atctggtaat tgttggaaat agacagaaca ccgttattct gaaggccttc atacgtgttc
     4621 cgtccctggt aattctgctc tgcctgggtc tgataggtat agccggtgtt aagcttataa
     4681 gtcagcccct tcatcggaag gttgatatct atataattat ttgagaccag tacattcttg
     4741 tggttaacat taatgtcgtt aagtgaactg agcggatttc tggcatagct tgcatcctcc
     4801 catgtggaga gtctgagttc accagattct gtatacgccc tgccaagcgg attcatcgta
     4861 aaggcatcat cgaaatctgc agcattgcca cttctgttat aaaacccata ctgtgtactg
     4921 gtaccaattg ttacatattt gtttaccgtc tgatcaaagt tgattctgag cgtaattcta
     4981 ttgaagtcgt cgtttttggc tatccccttg ttcatggtgt agtttccgga aataaaatat
     5041 ctggatttgt tgccggaacc tcttaatgaa aggttgtgct gctggttaaa accgttccgg
     5101 agagcgagat ccacccaatc cgttgttgca ttctgtgcat acatttctgc ctcagtaaat
     5161 gaatgaacaa gacctgcgtc aagtttcatg gcaccgaaag tcggaccgtc catcatcttc
     5221 gggatgttaa tagctgttga tgaggtcacg ctcgcgtcat atgaaagctg gaccttttct
     5281 ccatcctttc cctttttggt ttgaatcagg attaccccgt ttgcccccct tgcaccataa
     5341 atggccgacg aggaagcatc cttgagtatc tccatggatg caatatcatt gggattgatc
     5401 tctgcccagt tgccggagta tggcactccg tccaatatga caagtggagc attggatgcc
     5461 gtaatggatc ggttgcccct gatacgcgtg gtggtggctg atccttcagc atttgatccc
     5521 gttatgctga tgctcagacc tgcaaccgaa ccctgaagag attgcaggag attggcactt
     5581 ggccgactct ccagggtttc aggacttact gaagcaaccg aaccggtaac atcactcctt
     5641 ttctggacac catagcctac aactataact tcatccagaa ccatctcatc aggaaacaaa
     5701 acaatattga tctccctttc atctgtaacg ttatattcaa caggtttgta gcctatgaat
     5761 gagaacagta acatttcacc tgtccgggca gttatggaga acatgcctgc ggcatcagtg
     5821 aatgtgcccc gctgggtacc tgcaacctga acggttacac ctgcaaatgg ttcatttttt
     5881 tcaccatctg taacctttcc ccttatctgg gcagtggcca gcagaccggg aaaaagtaac
     5941 aaaaacagaa tgatagtttt cttcattttt acactttttg agttaagtta attatcggtt
     6001 cctctcttca atacggtgcc agggcagggt atcaccctga aatgttgttg tgaccctgta
     6061 ggaaacaaat ccgtttacgg cttccggctc cattactgag attacataat ttgcgttttt
     6121 ctgcctcaga tcaacatagt atgacccttt tgcaaatggc agtcgctgag aaatcagcct
     6181 ggctgacact gaggcggtcc tgatcccttc ccactcagtt tctgactcct catatttctc
     6241 aatgaggtag ctctgtactt ttaacttttt gcctttcctg agctgtgtaa tctcaaggcc
     6301 taacagttta agtacttcta tctccttctc atgcccgggt tcaattatat agccttttgg
     6361 tcttggccgc acaatttccg gattcaggtt ctctgcatcg ttcaccctca tccggagctt
     6421 caatagcatt ccatctgaaa gatcaatgaa atcaacatcc ctgtaacata ctgatggcgt
     6481 actggtaaca acgacatcgg ttttcccgga caatgtctca tttcttgcct cattgataat
     6541 agtagtcagc tcttccttat gtctcaatgc acttagtaag aattcaccgg caacaaccct
     6601 ggcactatga gttctgcgtg caaaagaggt tcttcccagc cctatgccac gcgtctcaac
     6661 aagaagtgaa acggcgttag agagagcgta tgaagttgaa cttgaatgcg ggctctgtgc
     6721 ccctttattt aacataatac tgtcaccata gtcgcctgca gtgaagtaaa aattatgagt
     6781 gtatccattc ctttcaagag ctgcctcagc ctctttctgg aaaatatcca cagaggcctt
     6841 gcggagtccg gaaggtatat tgagataccc ggaaggaaga aacaatacgt catatgagat
     6901 ggaggctcca tcctcgccga tacctgcata ttccctcctg gtaggctgaa attcgtggaa
     6961 atcgaaggcc agatcggctt gccagctgat gaaggctgct tttataatcc ggctaaccgg
     7021 atcggcaaac ttagtctgat cgcgattaag gtcatagccg ttcgaggagc gtctggtatt
     7081 tgcaatataa ccatcgatat tcgcaatagg cagaatagca atatccaggt tatcaaggat
     7141 attctcccct tctgccgttg tcagtatctc ctttgcgagg aagagaagag cttcaccgcc
     7201 tgccggttca tttccatgaa gcaggccctg gaaccacacc cttaattttg gtgcagaaga
     7261 cccgtttgaa agatagatga ccggtatatc ctgcccaagg ggtgtagtgc ctattattct
     7321 tacactggca ttaacatgaa cagcaatatt gctctctatc cagtttagta tctcctcata
     7381 tgtggcaaat ctgttttcat cgagagatag tgttggagta tcaaacttca catcagggtc
     7441 tttaaagtac ctttgtgtga taaggccgct ctgctgccct gaaagcattg tgcagaaaaa
     7501 gaaagccaga atcagaataa cctggttttt gtgtgttctc agttgtcggg gatccatttt
     7561 catagaagaa tatatgacag aagtaccaaa agcagtgata ttgacagcaa tggtccggca
     7621 tttcgttttg caatgtcaaa tggagaaact ccggctatct cggagaccgc aataatcaca
     7681 atcgcaatgg gagaggccgc ccgcccaagg ccagccgcca gctggacggg aatcatgaat
     7741 gtaattgaag gtatgccgaa tttaagggca agatcaggta tcaactgccc gattgtagtg
     7801 aaggaagcta ctccgctgcc ggtgatcagt gatgccgaga atgaaatgag tgtcagcagc
     7861 aatgtcacaa atactgagtt cagccccaat gcctgaattg cggtaaccat
//
LOCUS       DAOOYG010000124         7799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10459_length_7799_cov_7.100981, whole genome shotgun sequence.
ACCESSION   DAOOYG010000124 DAOOYG010000000
VERSION     DAOOYG010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10459_length_7799_cov_7.100981"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PK288_09565"
     CDS             complement(<1..853)
                     /locus_tag="PK288_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HPM19213.1"
                     /translation="MKLLDKLKLNARKYNMRIVLPEGYEERTLKAADIVIEEEIAQII
                     LIGDPAKISESSEKYGLRNISRARVVNPVSHDRKDDYIKMMVEMRKGKGVTAEIASDL
                     IEDPLYLAAMMIKAGDADGEVAGAEHSTGAVLRPAFQYVKTAPGISIVSGAFLMIHPN
                     RSFGADGVFIFADGAVHPDPTAQELAEIAVATAHTARTIGGFEPVVAMLSFSTKGSAR
                     HEMVDKVINATRIAREMAPGLTIDGELQVDAALVEEIGKKKAPGSSIPGKANVLIFPN
                     LESGNIAY"
     gene            1043..3976
                     /locus_tag="PK288_09570"
     CDS             1043..3976
                     /locus_tag="PK288_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008664789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPM19214.1"
                     /translation="MTFLGERLDQLKEILEGELSYDIVSRTIYSTDASDYKEMPLAVA
                     WPKGISDLKKILRLASEEGIGLTMRAAGTSLAGQVVSSGIIADVSRHMNRIIEFNKNE
                     KWVRVEPGVVLDELNNFLKPSGLFFAPETSTSNRCCLGGMVGNNACGSHSLVYGSTRD
                     HTIEVRALLSDGSEALFRPVDRKAFREKCKLPNLEGAIYRNIRDILSDDHNRKEIIEE
                     YPDPSIPRRNTGYALDLLLDSEIFNNDSTKPFNFCKLLAGSEGTLAIITEIKLNLVPL
                     PPGHKALVCVHHRERDEALHANLIALRFNPVAVELTDDRILSLTKDNLSQRENRFFIE
                     GDPGAIMIVEFAFHSEEETDSAAAGLINALRAAGYGYSYPVVKGNDMKKVWALRKAGL
                     GILGNMKGDRRPVSLVEDTSVKVEDLPAYMREFSEMLSSYGKDSVYHAHIGTGELHLR
                     PVLNLKDPADVELFRTIGLETARLVKKYRGSLSGEHGDGRLRGEFIPLMLGEHNYGIL
                     RRIKKCWDPGNILNPGKITDTPSMNTRLRYEPGMVTREIETIYDFSSTDGIVRAAEKC
                     NGSADCRKSAAIGGTMCPSFMATMDEKCSTRARANLLREFLSREDSDPWNHREVHDIL
                     DLCLSCKGCKSECPSGVDIAKLKSEYLQHWYDRHHVPLRTILIAYISTFNRIGSFIPG
                     IYNYFLSNGFFSGIFKKITGFAMQRSIPLIYKTSLRRWIRQNLTKMNPDDARGTVCLF
                     VDEFTNYNDTETGIAAIRLLTALKYRVVTVNHDNSARTFISKGLLRKAKRISRKNIRI
                     LGEIIDETLPLIGIEPSAILGFRDEYPDLAGDELREKALKIAENCYLIDEFIYREFKA
                     GRISRGSFTSKGLEILLHAHCHQKALSSSSSSIGMLSIPENYTVREIPSGCCGMAGSF
                     GYEKEHYELSQKVGELILFPEIRNSGMDVAIAAPGTSCRHHIQDGTGRKAKHPVEILL
                     EAFIE"
     gene            complement(4093..4863)
                     /locus_tag="PK288_09575"
     CDS             complement(4093..4863)
                     /locus_tag="PK288_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPM19215.1"
                     /translation="MKLTLLQSDIIWENKTDNLSRLESLISGAPAGTEMIFFPEMFNT
                     GFSMNPDNLSEPPGSLTTNWLLNVAARFNVGLGGSFIVQENGCYYNRWLFVSPDGTIR
                     KYDKRHLFSPANENKLLCRGNERIVFKFKEFRICPTVCYDLRFPVWSRCRKDYDLLVN
                     SANWPQSRRDAWITLLRARAIENQCYVAGINRIGIDGTGISYSGDSMIIGPKGEIIAS
                     GEQGSECLVSADISLEKLSEFRNRFPFLDDGDVFTMES"
     gene            complement(4990..6141)
                     /locus_tag="PK288_09580"
     CDS             complement(4990..6141)
                     /locus_tag="PK288_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19216.1"
                     /translation="MKKSLLITIIILAIILILPLVNLVRWYFQEKKPIDIVILDKTVP
                     TLERLKHKSLVWILTNDRFIRKDKKKSYSYRKDYYGFAPTRPLKERGSTKKEYHLSDM
                     MTLPEQSDALYIADTYGIFFNDWFAGVNKSRKSRKIYGGLNNTDYLLINEMKNRNKLI
                     ILEYNSFDFPTAAFESYRTQEKLGISFTGWTGCYFEKLDTTSKGFPIWLAAMYRKQYR
                     KPWTFTKPGLVLLTEKNIVVLEEGTHLTKALPRIVTDSANCRKYGVPSSVVFRNWFDI
                     IDPLENNVISRFRFETTALGDTLLSSNMIPNEFPAVVQEPVNQRTYYFSGDFATNRVW
                     YCTSRLKGFGNLKGLLYSDKPDDPRRFFWLYYRPLLNTIFNDYYLSLKK"
     gene            complement(6285..>7799)
                     /gene="ggt"
                     /locus_tag="PK288_09585"
     CDS             complement(6285..>7799)
                     /gene="ggt"
                     /locus_tag="PK288_09585"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HPM19217.1"
                     /translation="AAVAIEFALAVCYPEAGNIGGGGFMIYRDPSGRTDMVDYREKAP
                     LKAHRDMFLDPEGSVVPGLSTDTRLASGVPGTVDGMINIHGKYGLLSFRDVIQPAIDL
                     ARNGFPLTDEQAADLNGFRQKFIDRNSILPAFVRNDPWKAGDTLKQPELAETLERIRD
                     SGREGFYSGKTASLIAGEMEKGNGIIGLKDLAEYQAAFRAPVEGFYRGFRIVSAPPPS
                     GGAVILLQILGMVEPFQLKDLGFHSSASIHLLVEAEKRAYADRAEYLGDPDFTEIPVS
                     GLLDQGYLRKRMEDFREDTATPSSEIRAGHPLRHETEETTHYSVVDPMGGAVAATTTL
                     NGTFGSGIVIGGAGFLMNNQMDDFSLKPGTPNIYGLSGGSVNSVAGGKRMLSSMTPVI
                     LEKDGKLFMVAGSPGGSTIPTTVMQVITNVVDFDMDIANAVDAGRFHHQWLPDRIEYE
                     KTAFDRFTENKLKEIGHTLRERESLGRVNAIMVMPDGRLKAGADRRGLNSAYGY"
BASE COUNT         2097 a         1873 c         1770 g         2059 t
ORIGIN      
        1 tataagctat gtttccgctt tccaggttgg gaaaaatgag aacattggcc ttacccggaa
       61 tactgcttcc gggagccttc tttttcccta tctcctctac aagcgcagca tcaacctgaa
      121 gttccccgtc tatagtcagc ccgggagcca tctccctggc tatcctggtc gcatttatca
      181 ccttatcaac catttcatgc cttgcgctcc cctttgtcga aaaactgagc attgctacaa
      241 caggttcaaa gccgccgatg gttcttgctg tatgtgctgt ggcgacagca atctctgcga
      301 gttcctgtgc agttggatca gggtgaacag ccccgtctgc aaaaataaat acgccatcgg
      361 ccccgaacga cctgttggga tggatcatca gaaaggcgcc cgacacaatc gaaatgcccg
      421 gggcagtctt tacatactgg aatgccggac gaagcacagc acccgttgag tgctcagcac
      481 cggcaacttc cccgtctgca tcgccggcct ttatcatcat cgcggcgaga tacagcggat
      541 cctctataag atccgatgct atctcggcag tcaccccctt gcctttcctc atttcaacca
      601 tcattttgat gtaatcatcc ttccgatcgt gcgacaccgg gttaaccacc cttgccctgg
      661 agatgttccg aagaccgtat ttttcagaac tttccgaaat cttcgcaggg tcacctatca
      721 ggataatctg tgcaatctcc tcttcaatga caatatcagc agccttcagg gtcctttctt
      781 catacccttc gggaaggacg atccgcatat tgtactttct ggcattaagt tttagtttat
      841 caagcaattt cattgtgtat taatggttta ataatattca agatcatgct ttaaatgtac
      901 aaaaaaaaag tccgattaaa caaaaacgta agtcatgttt attgaaatca gtttcttttt
      961 ggcagaattc aggtgaaatg acatagttta gggaaggcag atcaatcctt ttcttaaatt
     1021 agcggactta aaccttcatt ctatgacttt tcttggcgaa agactggatc agcttaagga
     1081 aatcctggaa ggggagctta gttatgatat tgtttcaaga accatttatt cgacagatgc
     1141 ttcggattac aaggaaatgc ccctggctgt tgcatggccg aaaggtatct ccgacctgaa
     1201 aaagatcctc aggctggctt cagaggaggg aattgggctc accatgagag ctgccggcac
     1261 ttcacttgca ggtcaggtgg taagctcagg aataatagct gatgtctccc ggcacatgaa
     1321 caggataatt gagttcaaca agaatgaaaa atgggtcagg gtggagcccg gggtggtgct
     1381 ggatgaactg aataatttcc tcaaaccctc cggcttgttt ttcgccccgg aaacatcaac
     1441 ttcaaacagg tgctgcctgg gaggaatggt tgggaacaat gcgtgcggct cacattcgct
     1501 tgtctacggg tcaacccgcg atcatacgat tgaggtcagg gctctgctga gcgatggcag
     1561 tgaagctttg ttccgtcctg ttgacaggaa ggctttcagg gaaaaatgca agctgccgaa
     1621 cctcgaggga gctatttaca gaaacataag agacattctt tcagatgatc acaacaggaa
     1681 ggaaatcatt gaggaatatc ctgacccgtc aattcccagg cgcaataccg gatatgccct
     1741 ggatctgttg ctcgactcgg aaatcttcaa taacgattca acaaagccgt tcaatttctg
     1801 caagctgctt gcaggatcgg aaggaacact cgcaatcatc actgaaataa agcttaacct
     1861 ggtaccttta cctccggggc acaaagccct ggtatgcgtt caccacaggg aaagggacga
     1921 agctctgcat gcaaacctga ttgcattgag attcaatccg gttgccgttg aactgactga
     1981 cgacaggatc ctttcactta caaaggataa tctgagtcag agagaaaaca gattttttat
     2041 tgagggggat ccgggggcaa taatgatagt ggaatttgcc ttccattcgg aagaagaaac
     2101 ggattctgcc gcagcagggc ttattaatgc cctcagggcg gcaggctacg ggtattcgta
     2161 cccggttgta aaagggaatg acatgaaaaa agtctgggcc ctgaggaaag ctgggctggg
     2221 gatactggga aatatgaagg gagaccgcag gcctgtatcg cttgtggaag acacatccgt
     2281 gaaggttgag gaccttcctg cctatatgag ggagttttca gaaatgcttt ccagctacgg
     2341 caaggattcc gtttaccatg ctcacattgg caccggcgaa ctccatctgc ggccggtact
     2401 gaatctcaag gatccggccg acgtggaatt gttcaggaca attggtcttg agactgccag
     2461 acttgtaaag aaataccggg gatcgctgag cggtgaacat ggcgacggta ggctgagggg
     2521 tgaatttatc ccgctcatgc tgggggaaca taattacggt atcctcagga gaatcaagaa
     2581 atgctgggat cccgggaata ttctcaatcc cggaaaaata accgatactc catcaatgaa
     2641 taccaggctg agatatgagc cgggtatggt cacccgggag attgaaacaa tttatgattt
     2701 ttcatcaact gatggtattg tcagggctgc ggaaaaatgc aacggatcgg ccgactgcag
     2761 gaaatcagct gcgattggag gtacaatgtg ccccagcttt atggcaacaa tggatgagaa
     2821 atgctcaacc agggccaggg caaatcttct cagggagttt ctaagcaggg aggattccga
     2881 tccatggaac cacagggaag tccacgatat cctcgatctg tgcctgagct gcaagggatg
     2941 taaatccgaa tgcccttcag gcgtcgatat agcaaaactc aagtcggaat acctgcagca
     3001 ctggtacgac aggcaccatg tcccattaag aacaattctg attgcttata tttcaacctt
     3061 taacaggatc ggatcgttta tccccggtat atataattat tttctcagca acggtttctt
     3121 ttcaggtatt ttcaagaaaa ttaccggctt tgcgatgcaa agatccattc ctcttatata
     3181 caaaaccagc ctcagaaggt ggatccggca aaaccttaca aaaatgaatc ccgatgatgc
     3241 caggggaaca gtttgcctct tcgtggacga gtttacgaat tacaatgaca ctgaaaccgg
     3301 aattgctgca atcaggctgc tgactgcatt gaaatacagg gttgtaacgg ttaatcatga
     3361 taacagcgca agaactttta tttccaaagg attattaagg aaagcaaaaa ggatttccag
     3421 gaaaaacatc aggatactgg gagaaataat agatgaaact cttcctctga tcggcattga
     3481 accttcggca atactgggat tccgcgatga atatcccgat cttgcaggcg atgagctgcg
     3541 ggaaaaagcg ctcaaaattg ctgaaaactg ctacctgatt gatgaattta tatacaggga
     3601 attcaaagcc ggcagaatca gccgcggatc ttttaccagt aaaggacttg agatactgct
     3661 tcatgcccac tgccatcaaa aggcactttc ttcgtcatcc tcctcaattg gcatgctctc
     3721 cattcctgaa aactacaccg tcagggaaat cccatcgggg tgctgcggta tggcaggttc
     3781 attcggatat gaaaaagagc attacgaact gtcgcagaag gtgggagaac ttattttgtt
     3841 ccctgaaata aggaacagcg gaatggacgt ggcaattgcc gctcccggaa ccagctgcag
     3901 gcatcatatt caggacggga cgggcagaaa agcaaagcat cctgtggaaa tactgcttga
     3961 agcctttatt gaataaatcc cggatatcgt ccggctgcaa tgacatcacg gctgtatatg
     4021 ccaacaatac agaaaccggc atttgtccga ccagggagtt cagtttagat cataaaatcc
     4081 ggagtgtatt tttcatgact ccatcgtaaa gacatcacca tcatccagaa acgggaacct
     4141 gttcctgaat tccgacaact tttccaatga gatatctgca gagacaagac attctgaacc
     4201 ctgctcaccc gatgcaatta tttcgccttt gggcccgatg atcattgaat cgccggaata
     4261 actgattcct gtcccatcaa tgccaatcct gtttatcccg gccacatagc actgattctc
     4321 gattgccctt gccctgagca gggtaatcca tgcatccctc ctgctctgcg gccagtttgc
     4381 agaattgaca agcagatcat agtcctttct gcacctggac cagaccggga aacgcaggtc
     4441 gtagcagacg gtgggacata tcctgaactc cttgaactta aatacgatgc gttcattacc
     4501 cctgcacaga agcttgtttt cgtttgccgg gctgaaaaga tgccttttgt catactttcg
     4561 aatggttccg tccggtgaaa caaatagcca tcggttataa tagcatccgt tttcctgcac
     4621 gatgaaactt cctccgaggc cgacattgaa cctggcagcc acattcagca gccagttcgt
     4681 ggtcagcgat ccgggaggtt cactaaggtt gtcaggattc atggaaaatc cggtattgaa
     4741 catctcaggg aaaaagatca tttcagttcc cgcaggtgct cctgaaatca ggctttcaag
     4801 ccttgacagg ttatccgttt tattttccca gattatatct gattgcaaaa gagtaagttt
     4861 catttccggc aggtgctttc aaccagtttc gtccaatgtg acgggaaaaa caattgggat
     4921 gggggttaca tattagtgaa aggggccgta aaggtcccgg cattgaaaaa ccgctgtcac
     4981 tgaaagtttt tactttttca gggaaagata gtaatcattg aaaattgtat tcaggagcgg
     5041 cctgtaatag agccagaaaa atcttctcgg atcatccggt ttgtcagagt acagcaatcc
     5101 cttgagatta ccaaaaccct tgagccttga cgtgcaatac cataccctgt tggttgcaaa
     5161 atctcccgag aaataatacg ttctttgatt aaccggttcc tgcacaaccg caggaaattc
     5221 attcggaatc atatttgagg aaagcagcgt gtcgccaagg gcagttgttt caaaacgaaa
     5281 cctggaaata acattgttct caagcggatc gatgatatcg aaccagtttc tgaatacaac
     5341 agaagacggt accccgtact tcctgcaatt ggcggaatca gttactatcc gtggaagggc
     5401 tttggtaaga tgcgttccct cttcaagtac cacaatattc ttctcggtaa gcaggaccag
     5461 acccggcttc gtgaaagtcc agggcttacg gtattgctta cggtacattg ccgccagcca
     5521 tataggaaac ccttttgaag tagtgtcaag cttttcaaaa tagcatccgg tccagcctgt
     5581 aaaggaaatt cccagctttt cctgggtacg gtatgattca aatgcagctg tgggaaaatc
     5641 gaatgagttg tactcgagaa ttatcagctt attcctgttc ttcatttcat ttataagcag
     5701 ataatcggta ttgttcagcc ctccgtatat tttcctcgat ttcctgcttt tattaacacc
     5761 cgcaaaccaa tcattgaaaa aaatcccgta cgtatcggca atataaaggg catcagactg
     5821 ttcggggaga gtcatcatgt ctgagagatg atactctttt tttgtactgc ccctttcctt
     5881 cagcggtctg gtcggtgcaa acccgtagta atcttttctg taggaatagc ttttcttctt
     5941 gtctttcctt ataaacctgt catttgtaag tatccagacc aatgatttgt gtttcagtcg
     6001 ttcaagtgtg ggaacggtct tatcgagtat cactatgtca attggtttct tttcctgaaa
     6061 ataccaccgg accaagttaa ccaaaggcag aataaggatt attgccagga taataattgt
     6121 tatcaggagg gactttttca taatttatat ctgacaaaat tcaaatactc atgtaatctt
     6181 aatcttagac agatttaaga aataactgtt aaatgtatca atttattttt cacacaggta
     6241 aatattttta ctcaaagtgg aaaaaaccga taaaatatat tgccctaata tccataggca
     6301 gagtttaatc cgcgcctgtc ggcacctgcc ttcagccttc cgtcgggcat gaccattatt
     6361 gcatttaccc tgccgagcga ttccctctcc ctgagtgtat gcccgatctc cttcagtttg
     6421 ttttcagtaa aacggtcaaa tgccgtcttt tcgtattcaa tccgatcagg aagccattga
     6481 tggtgaaatc ttccggcatc aactgcatta gcaatatcca tgtcaaaatc cacaacattt
     6541 gtaattacct gcataactgt tgtaggaatt gtggatccac ccggcgatcc tgcaaccata
     6601 aacagttttc catccttttc gagtattacc ggggtcattg aactcagcat cctcttacct
     6661 cccgcaaccg agtttacgga gccgcctgaa agtccgtaaa tattcggtgt gccgggtttc
     6721 agtgagaaat catccatctg gttgttcatg agaaatcctg cacctccgat cactattccg
     6781 cttccaaatg ttccgttgag ggtcgttgtg gcggcaactg ccccacccat tggatcaacc
     6841 acggaatagt gggtagtctc ctcagtctca tgcctcaacg gatgacctgc ccgtatttcc
     6901 gacgacggag ttgcagtatc ttccctgaaa tcttccatgc gctttcggag ataaccctga
     6961 tccagcagcc cggaaacggg aatttcagtg aagtcgggat ctccaagata ttctgccctg
     7021 tcggcgtaag cccttttttc tgcttcaacc agcaggtgga ttgaagctga tgaatgaaat
     7081 cccagatctt tcagttgaaa cggttccacc atcccaagga tctgaagcag gatcacggct
     7141 ccgcctgagg gaggtggtgc tgaaactatc ctgaatcccc tgtaaaaacc ctccacgggg
     7201 gctcggaaag ccgcctggta ttcagcaaga tccttaagcc cgataatacc attccccttt
     7261 tccatctctc cagcaatcag gctggcagtc ttccctgaat aaaatccttc cctcccggaa
     7321 tccctgatcc tttccagggt ctctgccaat tcgggttgtt ttaaggtatc gcccgctttc
     7381 cagggatcat ttcttacaaa agccggtaat atactgttcc tatcaataaa cttctgccgg
     7441 aaaccattca ggtcagccgc ctgttcatca gtcaggggaa agccattcct tgcaaggtcg
     7501 atggcaggct ggatcacatc cctgaatgac agtaaaccat atttgccatg gatattgatc
     7561 attccgtcca ctgttccggg tacccccgag gcaaggcggg tgtcggtgct caggccgggc
     7621 acaacggatc cttccggatc gagaaacatg tccctgtgtg ctttcagggg agccttctcc
     7681 ctgtaatcta ccatatcagt cctccctgac ggatcgcggt agatcatgaa tcctccccct
     7741 cctatatttc cggcttccgg gtaacaaaca gccagtgcaa attcaatggc cactgcagc
//
LOCUS       DAOOYG010000125         7740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10597_length_7740_cov_7.296682, whole genome shotgun sequence.
ACCESSION   DAOOYG010000125 DAOOYG010000000
VERSION     DAOOYG010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10597_length_7740_cov_7.296682"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PK288_09590"
     CDS             complement(<1..1016)
                     /locus_tag="PK288_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19218.1"
                     /translation="MSRILEYLRITPSDQSVDYVENKKQFHLFTLLTEQRHSKTLSLS
                     SVIADDTEKGLRMLLSVDEDITSKIEKLCMNPELLEIAVNAIHSAIITGKKIYFYGCG
                     ATGRLAKQMESTFWRPFWRKLRQSEYWIKLEPHLPSDIEERLIGEMTGADRALISSLE
                     GFEDLQLIGKLQLYDRKIEKGDAVFCVTEGGETSSVIGTILAARKQYGDAAGRADSEA
                     SGKLFFAYNNPDELLLPFDRSRSVIENKGITRLNLTTGPQGITGSTRLQATTSETFIL
                     GAVMEEALYRILRGFLNDRELADLGFEKGFGLTDRLLSFGEVKKSVDNAASGIARFTE
                     LEASA"
     gene            complement(1143..4373)
                     /locus_tag="PK288_09595"
     CDS             complement(1143..4373)
                     /locus_tag="PK288_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPM19219.1"
                     /translation="MKRFIVCIAAVLFILTSCKRYSDYTNIPFSEKEPRDWENPAVFN
                     INGEAYHASFISYPDENSIIQDDKTVSPNYLSLDGIWKFHYADSPDKRPFWFFKDDFD
                     TRKWDNIEVPSNWQMKGYDVPIYVNADYPFKKNPPYIEHSWNPVGSYKRTFTVPASWK
                     KKDVFLQFGAVSSAFYVWVNEQLVGYRQESKLPAEFNITKYLRKGKNSLSVEVYRWSD
                     GSYLEDQDFWRLSGIQRSVFLHARPKTRIVDFFAVADLENNYRDGNLKLSVELGSTET
                     NDSDLSLKASLFDGNNEIYSEEKKTGLNGSKAFLEFNGLFPSVKRWTAETPDLYTLVL
                     ALKDRSGKVLECAGARIGFRKVEIKDSRLLINGVPIYLKGVNLHEHHDVTGHVIDEAT
                     ILKDIQVMKSNNVNAVRTSHYPQQELWYDMCDKYGLYLIDEADIESHGMGYDKDVTLA
                     DKAEWAAAHLDRMQGMVERDKNHPSVIIWSLGNESGDGHNFLNNYKWTKNRDVTRPVQ
                     YEREGEQTNAPERHSDIVCPMYSKIEFIEKYAQDPKNDRPLIMCEYAHSMGNSTGNLQ
                     DYWDVIEKYPLLQGAFVWDWVDQGLLKTDANGINYWAYGGDYGEEGIPSDGNFCINGL
                     TWPDRTGKPGLSEVKKVYQYIGFEPVDLKNGRIRVINKYDFITLADFNILWTVYSDGI
                     AVKSGKLAFPHLLPHHDTVAAVAFGDIEPAPGTEYFLNLMATRSDEWNLIPPDHIYAA
                     EQFRLPVEGKPLFGKKEELLVLQTKTEDKIFVVNGEGFTVRFDLEKGRMASFSSGDRE
                     LLIQGPEPDFWRAPTDNDYGYGMDVKLGVWKKAGEKAAVRKVNITQPEMDRVIVAMEY
                     DIPGSGGEKIGGYVSTFTVLGSADVIVKNQFSKVSEKIPEIPRMGMQMRIPEIYSNLR
                     WFGRGPHENYCDRKTSAFTGLYESSVDDQYVPYIRPQENGYRTDTRWLTLTDEDGNGL
                     LVSGLPMICFAALRNIHDDFESPGRLSQYRRDAKTANTHTNDVNPRDFINLNVDYGQM
                     GVGGDDSWGAEIHPKYRLMDRKYEYSFRMRPVKSGDDILKLAKERF"
     gene            complement(4555..5187)
                     /locus_tag="PK288_09600"
     CDS             complement(4555..5187)
                     /locus_tag="PK288_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPM19220.1"
                     /translation="MKRFALYLFLVLMTLNDPVAEGQSKYYHRQQPVSGYGIKAGINY
                     AGQSTTNRTAEFKTSGIIGVNAGGYFNYFFLRPVALQAELLVSGKGSHWKDYYDEAKD
                     FVTYIDLPLLIRYQPIQFLNLHAGPQAGLRLGAKQKDMKTGIQSDIKDYYKTFDFGFV
                     FGAEADLPHNLNITVRYIMGISSATTRVRYVDPWMNNLLQISLGYRLAGR"
     gene            complement(5321..6997)
                     /locus_tag="PK288_09605"
     CDS             complement(5321..6997)
                     /locus_tag="PK288_09605"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPM19221.1"
                     /translation="MKKDIEIAQAARIKPIAEIAEILGIPGKEIEPYGKYKAKIPLSF
                     INDENVKKARLILVTATTPTPPGEGKTTTSIGLAQAMNRLGKKTTVVIREPSLGPVFG
                     IKGGAAGGGYAQVIPMEDINLHFTGDISAVEKAHNLLAALIDNNIQLKTKDGNIGIDP
                     RTIEWKRVMDMNERSLRDIVIGLGGITEGVPRRSGFDITAASEVMATLCMARSLPDLK
                     ERLGNIFIGYTYDEKPVFARDLKANGAMAALLKEAIKPNLVQTLEGTPAIIHGGPFAN
                     IAQGTNSIIGTKMGMSLSDYVVTEAGFASELGAEKFLDIKSRTGNLKTDAVVIVTTVR
                     ALKYHGGAKLSALQTEDLDALKNGFDNLDKHIENMRYFGFRPVIAINKFETDTEQELK
                     MLINYCESQHLKVASNESWLKGGEGAEELAQKVLEQISEGNDAYNPLYELEWSFEKKI
                     ETICKKMYGADHVEYTVKAKNQLDRIEQIGFGNLAVCIAKTQKSLSDDQTKRGRPRGF
                     TVKIREVELSSGAGFIVPIAGTIMRMPGLPNSPSAEKVDIDSEGRISGLF"
     gene            complement(7257..>7740)
                     /locus_tag="PK288_09610"
     CDS             complement(7257..>7740)
                     /locus_tag="PK288_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPM19222.1"
                     /translation="FIMISTFGCNHGLILAGPRVYYAMACDGLFFRKVGEINKNGVPG
                     FAIAVQGIWSVLLCLSGTYSDLLDYVIFAVLLFFILTIMAIFILRIKKPDLPRPYKTF
                     GYPVIPAVYILATTFIMVVLLIYKPKFTFPGLVIVLLGIPVFYLWKKYSKNGSAESVS
                     "
BASE COUNT         1995 a         1889 c         1568 g         2288 t
ORIGIN      
        1 gccgaagctt ccagttcggt aaaccgggct atcccggatg cggcattatc cacgcttttt
       61 ttaacttcgc cgaacgaaag gagcctgtca gtcagtccaa agcctttctc aaatcccagg
      121 tcagcaagtt ccctgtcatt caggaacccg cgcaatatcc tgtacaacgc ttcttccatc
      181 acggcaccca ggatgaaagt ttcgctggtt gttgcctgaa gcctggttga tccggttatt
      241 ccctgcgggc cggtggtaag attcagcctg gttataccct tgttttcgat cacgcttctg
      301 ctccggtcaa atggaagcag aagttcgtcg ggattattgt atgcaaaaaa cagtttcccg
      361 gaagcttcgc tgtcagccct gccggcagcg tcgccatatt gcttccgggc agcaagtatt
      421 gttcctatca cggaagatgt ctctcctccc tcggttacac aaaacaccgc atcacctttc
      481 tctattttcc tgtcatacag ctgtaacttt ccgataagct gcaggtcttc aaatccttca
      541 agggaactta tcagggccct gtcagccccg gtcatctcac cgataagccg ttcttctata
      601 tctgagggca ggtgaggttc cagttttatc cagtattcag actgcctgag ctttctccag
      661 aaaggtctcc agaaggtact ttccatctgc ttggccagcc ttcccgtagc accgcagccg
      721 tagaaataaa tctttttccc tgtaattatg gcactgtgta tggcattaac tgctatctcg
      781 agcagctcag gattcatgca taatttttca atttttgatg tgatatcttc atcgacagaa
      841 agaagcatcc tgaggccctt ttcagtgtca tctgcaatca ctgaactgag agacagtgtt
      901 ttggaatgtc tctgttcggt taatagcgtg aaaaggtgaa actgcttctt attctcaaca
      961 tagtcaaccg actgatcaga gggagtaatc ctcaggtatt caagtatcct gctcatctat
     1021 tcttttttat gaattaacaa tcaatgttgt tgcgtactgc ttccaaccat gggacaagga
     1081 aaattacaaa caggctccga ttgcctgaca ttcaaaagat actgaaataa ttcaatcatc
     1141 ccttaaaatc tttccttggc gagtttcaat atatcatcac cgctttttac gggacgcatc
     1201 ctgaaactgt attcatattt tctgtccatc agcctgtatt tcggatggat ctcagctccc
     1261 caggaatcgt ctccgccaac gcccatctgt ccgtagtcaa cattaagatt tataaaatct
     1321 ctggggttca catcatttgt atgagtatta gccgttttgg catctctgcg gtattgtgac
     1381 agcctccccg gagattcgaa atcgtcatga atatttctca atgccgcaaa acatatcatg
     1441 ggcaaaccgc ttacaagcag tccgttgcca tcctcgtccg tgagggtcag ccacctggta
     1501 tcggtcctat aaccattctc ctgcgggcgg atatagggca catactggtc atccacgctg
     1561 ctttcataaa gtcccgtaaa agcagaggtt ttcctgtcgc aatagttctc atgcggtccc
     1621 cggccaaacc acctgaggtt ggaataaatc tctggtattc tcatctgcat gcccatcctt
     1681 ggtatctccg ggattttctc agaaactttc gaaaactggt ttttcacgat cacatctgct
     1741 gaacccagaa ctgtgaaagt tgaaacgtat ccgccaattt tctcccctcc tgatcccggg
     1801 atatcgtatt ccattgcaac aatcaccctg tccatttcag gctgagtaat attcaccttt
     1861 ctgactgcag ccttttcacc cgctttcttc cagacaccga gtttcacatc cataccatat
     1921 ccgtagtcgt tatcagtagg ggcccgccag aaatccggtt ccggtccctg aatcaataat
     1981 tccctgtcac ctgaactgaa tgaagccatt ctcccttttt caaggtcgaa cctgacagta
     2041 aatccctctc cgtttacaac aaatatttta tcctcggttt ttgtctgtaa aacaagcagc
     2101 tcttcctttt tcccgaagag tggttttcct tcaacgggca gtcggaactg ttcggcggca
     2161 taaatatgat caggcgggat caggttccat tcatcacttc tggtagccat taaattcagg
     2221 aaatattcgg tgccgggtgc aggttcaata tcaccgaacg cgaccgctgc aacagtgtca
     2281 tgatgcggga gaaggtgagg aaaggcaagt tttccggatt taactgcaat tccgtcagaa
     2341 tagactgtcc ataatatatt aaagtcagct aatgttatga agtcatattt attaataacc
     2401 ctgatccggc cgtttttcag atcgactggt tcaaacccga tgtactgata taccttcttt
     2461 acttcagata atcccggttt accagtccgg tcaggccagg tcaatccgtt aatacagaag
     2521 ttaccatcgc tcgggattcc ttcttcaccg taatcacctc catatgccca gtagttaata
     2581 ccgttcgcgt cagttttaag aagtccctgg tcgacccagt cccatacaaa tgctccctgc
     2641 agcaggggat atttctcaat aacatcccag taatcctgaa gatttccggt agaatttccc
     2701 atggaatgag catattcaca cataataagg ggtctgtcgt ttttcggatc ctgtgcatat
     2761 ttctcgataa attcaatttt tgagtacatc gggcaaacga tatccgaatg gcgttcgggc
     2821 gcatttgtct gttctccctc acgttcatac tgtacgggac gtgtgacatc cctgtttttg
     2881 gtccatttgt aattattaag aaaattgtgc ccgtcacctg attcattccc gagcgaccag
     2941 attatcacgg agggatgatt cttatccctt tcaaccattc cctgcatccg gtcaagatgg
     3001 gctgctgccc attcggcttt atccgcgagg gtgacatcct tatcataacc cataccatgt
     3061 gattcgatat cagcttcatc aatcaggtaa agaccgtatt tgtcacacat gtcataccac
     3121 agttcctgtt gcggataatg agaggttctg acagcattta cattattgct tttcattacc
     3181 tgaatatctt taaggatggt tgcctcatcg atcacatggc cggtaacatc atgatgttca
     3241 tgcaggttaa cccctttcag atagattggt acgccgttaa tcagcagccg ggaatccttt
     3301 atttccactt tcctgaaacc tatcctggca cctgcacact ccagtacttt acctgagcgg
     3361 tctttcaaag caagtacgag ggtatagaga tcgggagtct cggctgtcca tctttttacc
     3421 gaaggaaaca gtccgttaaa ctccaggaat gccttgctgc cattcaatcc tgtttttttc
     3481 tcttcgctgt agatttcatt attaccgtca aaaagcgatg ctttcaggga caggtcactg
     3541 tcattggttt ctgttgatcc tagttcaaca ctgagtttca ggttcccgtc cctgtaattg
     3601 ttttcaagat ccgcgacggc aaaaaaatca actatccgtg ttttgggacg ggcatgcagg
     3661 aagacgctgc gctggatccc actaagccgc cagaaatcct ggtcttcaag gtaactgccg
     3721 tcactccacc tgtatacctc cacggaaagt gaattttttc cctttctcag atattttgtg
     3781 atattaaatt ccgcgggcag cttgctttcc tgcctgtatc ccacaagctg ttcattgacc
     3841 cagacataga aggcggagct gactgcaccg aattgcagaa aaacatcctt ctttttccag
     3901 gaagcaggaa cagtaaaagt ccttttatag gatcccaccg gattccagct atgctcaata
     3961 taaggaggat tttttttaaa tgggtagtca gcattcacat agataggaac atcatagccc
     4021 ttcatctgcc agtttgaggg cacctcaata ttatcccatt ttctggtatc aaaatcatcc
     4081 ttaaaaaacc agaagggtcg tttatcaggt gaatctgcat aatggaattt ccagataccg
     4141 tcgagtgaca ggtaattggg agaaacagtc ttatcatcct gaattatgga attttcatca
     4201 ggatagctga tgaatgatgc atggtaagcc tccccgttga tgttaaaaac tgccggattt
     4261 tcccagtcgc gcggctcctt ttcggaaaaa ggaatattcg tgtaatcact ataccgttta
     4321 catgatgtta aaataaacag gacagcagca atgcaaacta taaatctttt cataaaagcc
     4381 aggtgttatt aagttatgtg ataatcatct gtatttatat cccggaccgt gaattatgac
     4441 acgcatgaag ggatcacatg atatcttgtc cgctcttctt caatcaaaaa tcatcagcat
     4501 ttccggcaaa caggttcttc cgcagttaaa gccgcattcc gcctgtaacc ggcatcatct
     4561 ccctgccagc ctgtaaccca gcgatatttg caacaagtta ttcatccagg ggtcaacata
     4621 ccttacccgg gtagttgcag aagaaatacc cattatatat ctcacggtta tgttcagatt
     4681 gtgcggaaga tcagcctcag caccaaaaac aaatccgaag tcgaatgttt tgtaataatc
     4741 tttaatgtca gattgaatcc ctgtcttcat atccttctgt tttgcaccca gcctgaggcc
     4801 cgcctgcgga ccggcatgaa gattcaaaaa ttgtatgggc tggtagcgaa tcagtaacgg
     4861 aagatcaata taggttacaa agtcttttgc ctcatcataa taatctttcc agtgggatcc
     4921 tttccctgaa accagcaatt cagcctgcaa agcaacaggt cgtagaaaga aataattgaa
     4981 atagccgcct gcattgacac ctataatacc ggaagtttta aattctgctg ttctgttagt
     5041 agtggattgt ccggcataat tgatacctgc ctttattccg taacctgata ccggttgttg
     5101 tcgatggtag tattttgact ggccttcagc aacgggatca ttcagggtca tcagaaccag
     5161 gaaaagatat agtgcaaacc ttttcatctt ttcataatta ataaacttca ggcatctaaa
     5221 gtaacaaaat atacagaacc aagacaaaaa gcagtgccgg cagggaaatt cctgttgctt
     5281 accgcaagca gcttttgtat ccgtaaccga agttacgtga tcagaataat cctgaaatac
     5341 gtccttcgga atctatatcg accttttcgg cagaaggaga atttggaagg cccggcattc
     5401 tcataatagt tccggctatc gggacgatga aacccgcgcc tgatgagagt tcaacctctc
     5461 ttatctttac ggtgaaaccc cggggtctgc cacgtttagt ctgatcatct gaaagtgatt
     5521 tctgtgtttt cgctatacat actgccaggt taccaaatcc aatctgctcg atcctgtcga
     5581 gctgattttt tgccttaaca gtatattcca cgtgatctgc accatacatt ttcttacaga
     5641 ttgtttcgat ctttttttca aatgaccatt caagctcata taatgggttg taagcatcgt
     5701 tgccttcaga aatctgttcc agtacttttt gagcgagttc ctcagctccc tctcctccct
     5761 ttaaccatga ttcattggat gccaccttga gatgctgaga ttcgcaatag ttaatcagca
     5821 ttttaagttc ctgctcagta tctgtttcga acttgtttat tgcaataacg ggtctgaacc
     5881 cgaaataccg catattctca atatgtttat cgagattatc aaaaccattc ttcaatgcat
     5941 ccaggtcttc agtttgcaaa gctgacaatt tggctccgcc gtgatatttc agtgccctga
     6001 cggttgtcac aattaccact gcatcggttt taaggttgcc ggtcctcgat tttatgtcga
     6061 ggaatttctc ggcaccgagt tcactggcaa aaccggcctc agtgacaacg taatcactta
     6121 gtgacattcc cattttcgtt ccaattatag agttcgttcc ctgggcaata tttgcaaacg
     6181 gaccgccgtg tatgatggca ggtgttcctt cgagagtctg aacaagattt ggtttgatgg
     6241 cttccttaag gagggcagcc attgctccgt ttgccttcag atcgcgtgca aaaacaggtt
     6301 tttcatcata tgtatagccg ataaagatat tccccaggcg ttccttcaga tccggcaggc
     6361 tgcgagccat gcagagtgtg gccattacct ccgatgctgc ggtgatatcg aaacccgatc
     6421 tccggggaac accctcggtt atacctccca gtcctattac tatatccctg agggaacgct
     6481 cattcatatc catcaccctt ttccattcga tggttctcgg atcaattcca atatttccgt
     6541 ctttggtttt cagttggata ttgttgtcta taagcgcggc caggagattg tgtgcctttt
     6601 caactgctga aatatcacct gtaaaatgaa ggttaatatc ttccatcggg atcacctggg
     6661 cataaccgcc tcctgcagcg cctcccttga ttccgaaaac aggcccaagg gatggttcgc
     6721 gaattacaac ggttgttttc tttccaagcc tgttcatggc ctgtgcaaga cctatggaag
     6781 tggtagtctt tccctcgcct ggtggcgtcg gggttgtcgc agttaccagt atcagcctgg
     6841 ctttttttac attttcgtca ttgatgaatg acaacggaat cttggccttg tattttccat
     6901 acggttcgat ctcttttccc gggataccga gaatttcagc aatctcagca atgggtttaa
     6961 tcctggctgc ctgggcaatt tctatatcct tcttcatatg caactaatat tgattttatt
     7021 gattcattca ggagagaaat cacacatgac tgttaagtat ccaacatcct ttggttccat
     7081 gccttggcca atcatgcaga tcaaaataat attcagttat ttaaagtcct aaatatagaa
     7141 aacttttcag cagttatgcg aggtaatcta aaaagattgt cactttatca taaatatcac
     7201 accacgaccc tgtttataag tgtgaatctt ttcacaccag ggaggatgaa ccctgatcac
     7261 gaaaccgatt ctgcactgcc gttcttcgaa tactttttcc acaggtagaa taccggtatt
     7321 ccaagcaaaa ctattacaag gccggggaag gtaaattttg gtttataaat aagcaggacg
     7381 accataatga acgttgttgc gaggatgtat acagccggta ttaccggata gccgaaagtt
     7441 ttgtagggtc gcggcagatc cggtttcttg atcctgagga tgaatattgc catgattgta
     7501 aggataaaga acaagagaac tgcgaaaatc acataatcca gaaggtcgct gtatgttcct
     7561 gacaagcaga gcagtaccga ccagattccc tgcactgcga tggcgaatcc gggaacacca
     7621 tttttgttta tctctcccac cttcctgaaa aacaggccgt cgcacgccat cgcataataa
     7681 accctcggtc ccgcaagaat tagtccatgg ttgcaaccga atgtactgat cattatgaac
//
LOCUS       DAOOYG010000126         7737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10604_length_7737_cov_5.855767, whole genome shotgun sequence.
ACCESSION   DAOOYG010000126 DAOOYG010000000
VERSION     DAOOYG010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10604_length_7737_cov_5.855767"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1127)
                     /locus_tag="PK288_09615"
     CDS             complement(195..1127)
                     /locus_tag="PK288_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPM19223.1"
                     /translation="MAVNFFYFLVFPGLLFLGVAGAFLSWFDRKVTAMVQFRKGPPVL
                     QPFYDFFKLLLVKETILPKYGSPVIFLLAPVFAVFGATMAGVFILLPLFNITTGFRGD
                     LLVIFYLLTIPSFSYILGSLASGNPLASVGSSREMKLILSYELTFMLVLAAVIMKCGR
                     QFDLYSIIAAQQASSPFAASVSGVLLFIVAIFCIQAKLALVPFDMAEAETEINHGVFI
                     EYSGAAYALIRLAKYIMLLVLPSLVAALLLGGFRLDGIHVLWSLVKVIGIVLLLTLIR
                     NTNPRLRIKQAVNFFLVWMNLLVVIAIVLIALGY"
     assembly_gap    1211..1310
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1416..1916)
                     /locus_tag="PK288_09620"
     CDS             complement(1416..1916)
                     /locus_tag="PK288_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPM19224.1"
                     /translation="MANKLLIDMIRLREYMIQNPDHEIPEGWFHKPFNQHGLKTVREL
                     AVFRFVCRKCEDAPCISVCPADALEKDSEGIINRHTNLCVSCKSCVTICPFGTMMTDF
                     FRHHRNKDLFHDLNNNEELKLFVESCPPGVASLVDHDEMPEKHIHKLNDKTLIRDYLY
                     PADQTQ"
     gene            complement(1916..2740)
                     /locus_tag="PK288_09625"
     CDS             complement(1916..2740)
                     /locus_tag="PK288_09625"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPM19225.1"
                     /translation="MTDDRNIYKPIKAKLTRVIDETPLIKTFVLVPETPFKFETGQFI
                     ELSVDGIGEAPFTPSSSPLVTDKLEVTVMKTGYVTEYMHRLEPGVFMGIRGPYGRGYP
                     LEKFYGKVVMILGGGCGLAPIRSLLYALEDVKDKLEKVILCYGSKTPADCVYKPLFDR
                     LNSIDKFEAYRTVDKSDEDWDGPVGVVTSLLNSIKYNRKNSVAVVCGPPVMMKFGTIK
                     FLEMGYRDDEIYLSMEKNMSCGLGKCGHCMMGRYFVCKDGPVFTWEEIKDQPQIWE"
     gene            complement(2747..3739)
                     /locus_tag="PK288_09630"
     CDS             complement(2747..3739)
                     /locus_tag="PK288_09630"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPM19226.1"
                     /translation="MQYLSIKKSDWDNHVDQLLLSFSVFAPVKNEYSLDYEEISSENI
                     SNISYNIPKPATPLKNFFLPVKENVTSEGISGKKRVILGSPGCDVAGLRLLDEIYLDR
                     EFNDIFYRSRRDNTIIIASDCLRIQEHCHCTAYGIRPYPEDGADLSVMLIDDVVLLRV
                     ISQKGIDFKNSFLPGLTPADDSLVSEAENNHRKIESMLRDKWVDLPDYSKTGELVAGA
                     KPGIWKKYSSRCVSCGACTVICPTCSCFLLIDKPGFEKIKQQDACQYPGFERVAGGED
                     PLFRLSDRFRNRYMCKYVWKPQKFRASACTGCGRCTEACIGKIDKNELFLELSK"
     gene            3926..4531
                     /locus_tag="PK288_09635"
     CDS             3926..4531
                     /locus_tag="PK288_09635"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPM19227.1"
                     /translation="MKKITKYVIKNSLKRIFLYRACLVRLKMMGVEKVFSYTLANETG
                     VTSDQVRKDFSEFGIRGNKRGGYEIDELLGKMEKIFHRNKDHNIVLIGMGNLGLALSK
                     YQRFVQRNMNIVATFDIDPFKQKIRSDIPVYSPDRMKEIIDRFRVRIAIIAVPEVSAQ
                     EVCDDLVRLGIKGIINFAPQLLKAPPEVIVNMLTCAMSWKV"
     gene            4608..6854
                     /gene="hypF"
                     /locus_tag="PK288_09640"
     CDS             4608..6854
                     /gene="hypF"
                     /locus_tag="PK288_09640"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HPM19228.1"
                     /translation="MRGLVQGVGFRPFICRLAARHGIHGEVKNMNNGVVVNTDCDRST
                     LDRFTGEIPRLAPPASRIKSIDVTGKEFPGFNGFRIVTGSRSDSQVTEISPDIAVCDE
                     CLAEMATDGFRIDYPFINCTNCGPRFSIITGMPYDRPATTMKEFKMCSRCRSEYDNIL
                     DRRFHAQPTACNNCGPRYLLKNSGKIFTGVADIVGEISVLIGSGKVVALKGIGGYHLM
                     CDALSEEAVEGLRGRKHRDAKPFAVMFRDLNAVRDYCFVDRLEERELVSWRRPIVLLR
                     CKRHLAAGVSNGLGTTGAMLPYMPFHHLLFRELKTPAVVLTSGNLSDEPIIIDDAEAE
                     ERLMSVADSFVSHNRRIHNRTDDSVVRIIGGNTAIIRRSRGYVPHPVDLGFNVDGILA
                     LGAGQKNTFCIGKGFQAFPGQYIGDLDNAATCDFYTESIDRFTGLFNFSPALIACDLH
                     PDYFSSRYASILEKKYNIPLVRVQHHHAHIASCMAEHGLDERVAGVCLDGTGFGTDGH
                     TWGAEFLVADRKEFLRFGHFDYVPMPGGDKAVAEPWRMALSYLYMYFGDTLNYRDIEP
                     FSKISGNEFMLVMEMIRKKINTPLTCGAGRLFDAVSALTGICPEAGFDSEAPMRLESA
                     IKCCTEKHYPYRVNGSVSFGDMFEAILNDIMVRNASVSLISAKFHNTMAQVILDMSER
                     IREEHSVDVVVLTGGVFQNKYLLERASEKLRKANFRVYTSNQVPANDGGISLGQMAVA
                     SKLSGLCA"
     gene            6857..7069
                     /locus_tag="PK288_09645"
     CDS             6857..7069
                     /locus_tag="PK288_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPM19229.1"
                     /translation="MPALVVSVNGDVAEVSVGGALFRAGLQMVENVSPGDYVLLHAGF
                     AIQKISEDEAMSTLKLLRELDDVSGQ"
     gene            7172..>7737
                     /gene="hypD"
                     /locus_tag="PK288_09650"
     CDS             7172..>7737
                     /gene="hypD"
                     /locus_tag="PK288_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPM19230.1"
                     /translation="MKYIEEFRDKDLIKKLIRHIGMVMRGKYVFMEVCGGHTSAIHRF
                     GIPSMLPSGISLISGPGCPVCVTAMDFIDRAIVLAGYSDVIIATFGDLIRVPGSSSSL
                     ERKRSAGADVRIIFSPLDALDIARENPGKKVVFAGIGFETTAPATAVTVLQAGKEKIG
                     NFYVLSAHKLMPPVMEAVAGGGTALDGF"
BASE COUNT         2007 a         1718 c         1871 g         2041 t
ORIGIN      
        1 tttaccagga ggacatccgc atgcctgatg ctgccgacca ggaggatacc aaacctttcg
       61 aggtcgtgcc tgggggcaag gcagtcgagg atctctatat cgcaattatt gcatgaggct
      121 cctccgaaat gaaagaccca gagtgacttt ttgaaacaat ggcttttaaa tcccacgatt
      181 cctgtttttt atccttaata tccaagtgca atcagtacta tggcaattac cacaagcaga
      241 ttcatccaca caaggaagaa attcactgcc tgttttatcc ttagtcttgg attggtgttt
      301 ctgatcaggg tcagaagcaa tactattcca atgactttta ccaggctcca taaaacatgg
      361 atcccgtcca acctgaagcc gccaagcaaa agagccgcga caagtgacgg aagaaccagg
      421 agcattatgt atttcgcaag cctgatcagg gcatatgctg caccggaata ttcgatgaat
      481 accccgtgat tgatctctgt ttctgcctcg gccatatcaa atggcacaag tgccagcttg
      541 gcctgtatgc agaatattgc aacgatgaaa agcagcacac ccgacacact ggctgcaaaa
      601 ggagatgagg cctgctgtgc tgcgataatg ctgtataggt caaactgccg gccgcatttc
      661 attatgacgg cggccagtac aagcataaat gtgagttcat agctcaggat cagtttcatt
      721 tccctcgaac tgccgacaga agcaagagga ttgcctgatg ccagggaacc caggatataa
      781 gaaaatgacg gaattgtaag tagataaaat atgacaagca gatcccccct gaatccggtt
      841 gtgatattga acagcggcaa caggatgaat acaccggcca tggttgcacc gaatactgca
      901 aaaacagggg caagcaaaaa gatcaccggt gatccgtatt tcggcagtat tgtttccttg
      961 acaagcagca atttgaaaaa atcatagaaa ggctggagga cgggaggacc cttgcggaac
     1021 tggaccatgg cggtcacctt cctgtcgaac cacgacagaa atgctcctgc cacccccagg
     1081 aaaagaaggc cgggaaaaac aaggaaataa aaaaaattca cagccattga tatagttgtt
     1141 tcgggtttcg ggttacgggt ttcgggttac gggtttcggg ttacgggttt cgggttacgg
     1201 gtttcgggtt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgggtttcgg
     1321 gttacgggtt tcgggttacg ggtttcgggt tacgggtttc gggtttcgga ttgcgtgttt
     1381 cgggttacgg gtttggggtt taatctcata tattgttatt gtgtttggtc agccgggtac
     1441 aagtagtccc tgataagggt tttatcattc agcttatgaa tgtgtttttc gggcatctcg
     1501 tcatggtcaa caagactggc caccccggga gggcacgatt caacaaaaag cttcaattcc
     1561 tcgttgttgt tcagatcatg gaacagatcc ttgttcctgt gatgccggaa aaaatcagtc
     1621 atcatcgttc cgaagggaca tattgtcaca cacgatttgc aggatacaca aagattcgta
     1681 tgacggttta ttattccttc gctgtccttt tcgagagcgt ctgccgggca tacgctgata
     1741 cacggcgcat cctcgcattt tctgcaaaca aacctgaata cggcaagttc cctcacggtt
     1801 ttgagcccgt gctggttgaa cggcttatga aaccagcctt caggaatttc atgatcggga
     1861 ttttggatca tgtattcccg cagccttatc atgtcaatca gcagtttatt tgccattatt
     1921 cccatatctg tggttgatcc tttatttctt cccaggtgaa gacaggcccg tccttgcaga
     1981 caaaatacct gcccatcatg cagtgaccgc atttgccaag accacatgac atgttcttct
     2041 ccatcgacag ataaatttca tcgtcccggt agcccatttc aagaaatttt atcgtaccga
     2101 atttcatcat taccggaggt ccgcatacaa ccgcaaccga gttcttcctg ttgtacttaa
     2161 tggaattgag cagactggtt acgacaccaa ccggtccgtc ccagtcttca tccgatttat
     2221 cgacggttct gtaagcttcg aatttatcaa tgctgttgag cctgtcaaac aggggtttat
     2281 atacacagtc agcaggcgtt tttgatccgt agcataatat tactttttcc agtttgtctt
     2341 taacatcctc aagggcgtac aggagcgatc ggataggagc cagcccgcag cccccgccca
     2401 gtatcataac caccttgcca taaaactttt ccagcggata ccccctgcca tatgggccac
     2461 gtatacccat gaacacaccc ggctccagcc tgtgcatgta ttccgtcaca tatccggttt
     2521 tcatcacggt tacttcaagc ttgtcggtaa caagcgggga ggacgaaggt gtgaagggag
     2581 cttctcctat gccatcgacg gataattcta tgaactgacc ggtctcgaat ttaaagggag
     2641 tttccgggac aagcacaaag gtcttgatca gaggcgtttc atcgatcacc ctggtgagct
     2701 tcgcctttat cggcttgtat atgttcctgt catccgtcat tatatattat tttgataatt
     2761 ccagaaacag ttcgttcttg tcaatttttc ctatgcatgc ctccgtgcac cttccgcagc
     2821 cggtacaggc agaggcacgg aatttctggg gtttccatac atacttgcac atataccggt
     2881 tcctgaacct gtcggacagc ctgaacagag gatcctcacc tccggcaact ctttcaaatc
     2941 ccggatactg gcatgcatcc tgctgcttta tcttttcaaa cccgggttta tcgatgagca
     3001 ggaaacatga acaggtcgga caaataaccg tacaggctcc gcatgacaca caccttgagg
     3061 aatatttttt ccagatcccg ggctttgccc ctgcaaccag ctctcccgtc tttgaataat
     3121 ccggaagatc aacccatttg tccctgagca tggattcgat ctttctgtga ttgttttctg
     3181 cttccgaaac caggctatca tctgccggtg tgagcccggg aaggaagctg tttttgaaat
     3241 cgatgccttt ttgtgagatc accctgagta aaacgacatc atcaatgagc atgacagaaa
     3301 ggtcggcgcc atcttccgga tagggtctga taccatatgc ggtacaatgg caatgctcct
     3361 ggattcgcag gcaatcggaa gcaatgatta tggtattgtc cctcctgctc ctgtaaaaaa
     3421 tatcgttgaa ttcccggtcg agatatattt catccagcaa tctcagacct gcaacatcac
     3481 aaccaggaga tccaaggatt acacgctttt ttccggaaat cccttcagat gtgacatttt
     3541 ccttcacagg aaggaaaaaa ttcttgagag gtgtggccgg ctttggtata ttgtaggaaa
     3601 tattgctgat gttttctgag cttatctctt cataatcaag gctatattca tttttgaccg
     3661 gtgcaaaaac agaaaaggac aacagaagct gatcaacatg attatcccaa tcactctttt
     3721 ttatgctcag atactgcatt tatgtgagat gattcacata taaaaatata atcaatgtaa
     3781 atacaagtca ataaaaaaaa gcatttttta ttgaataaaa atttattaat ttttaagata
     3841 tttgtaatca atggaataaa attattttat catatgtgaa attttttata tttttgattt
     3901 caggataatt tatcagaatt ataatgtgaa gaaaatcaca aaatatgtaa ttaaaaacag
     3961 cctgaaacgg atctttcttt atcgtgcctg ccttgtcagg ctgaaaatga tgggggtgga
     4021 gaaggtattt tcatataccc ttgccaatga aacgggagta acttccgatc aggtgcggaa
     4081 ggacttttcg gaattcggga tcaggggaaa caagcgggga ggatatgaga tagatgagct
     4141 tctcgggaaa atggaaaaga ttttccatcg caacaaggat cataatatcg tgctgatcgg
     4201 aatgggaaac ctcgggctgg cgctttctaa gtatcagagg tttgttcagc gcaacatgaa
     4261 tattgttgcg acatttgaca tagatccttt caagcagaag ataaggtccg acattcctgt
     4321 ctattcacct gaccgcatga aggagatcat cgacaggttc agggtaagga ttgcgataat
     4381 tgctgttccc gaggtatccg cccaggaggt atgtgacgac cttgtcaggc ttggaataaa
     4441 ggggatcatc aatttcgccc cccagcttct gaaagcccct cccgaagtga ttgtcaatat
     4501 gttaacctgt gcgatgagct ggaaagtgtg atttattacg ttcataagca aataaaggcc
     4561 gacggtcaga aaaatatcga attattatac agggatatga aatactgatc aggggactgg
     4621 ttcagggtgt gggctttcgt ccctttatct gcaggctggc agccaggcat ggtattcatg
     4681 gtgaagtaaa aaacatgaac aatggtgtgg ttgtcaatac cgactgtgac cggagtaccc
     4741 tcgacagatt caccggcgag attccccggc ttgctcctcc cgcctccagg ataaaatcaa
     4801 ttgacgtgac cggtaaggaa tttccgggtt ttaacggctt caggatcgtt acgggcagcc
     4861 ggtcagacag ccaggttacg gaaatcagtc ccgatattgc cgtatgtgac gaatgtcttg
     4921 ctgaaatggc aacagacggc ttcagaattg attatccgtt tattaattgt accaattgcg
     4981 gaccgcgctt ttcgatcatc acgggaatgc cttacgacag gcctgctact acgatgaagg
     5041 agtttaaaat gtgcagccgg tgcaggtcgg aatatgacaa cattctcgac cgacggtttc
     5101 atgcccagcc tacagcctgc aacaactgcg ggcccaggta tctgttgaaa aattcaggta
     5161 agatatttac cggcgttgcg gatattgtcg gggaaatatc agttctgata ggatcgggga
     5221 aggtcgttgc attgaaaggc ataggaggat atcatctgat gtgtgatgcc ctcagtgagg
     5281 aagccgttga aggacttcgt ggaagaaaac atcgcgatgc aaaacccttt gcggtaatgt
     5341 tcagggatct caatgcagtc agggattatt gtttcgtcga tcgtttggaa gaaagggagc
     5401 tggtatcatg gcgcaggccg atcgtgctgc tccgctgcaa aagacatctc gccgcaggtg
     5461 tgagcaatgg tctcgggacc acaggcgcca tgctgccata catgccgttt caccatctcc
     5521 ttttccggga actcaagacg cctgccgtgg tcctcaccag cggaaatttg tccgacgaac
     5581 caatcatcat tgacgacgct gaagccgaag agcggctgat gagcgtggcc gattcatttg
     5641 tgtcccacaa caggagaata cataacagga cagatgattc ggtggttcgc ataatcggcg
     5701 gcaacactgc cattatcagg cggtcgaggg gatatgtccc tcatcctgtc gatttagggt
     5761 ttaatgtcga cggaatcctt gccctcggtg ccgggcaaaa gaataccttc tgcatcggca
     5821 agggtttcca ggcatttccg ggtcagtata taggagatct tgacaacgcg gcaacctgtg
     5881 acttctatac ggaatcgatc gaccgtttca ccggactttt caatttcagt cccgcactca
     5941 ttgcctgtga tcttcatcct gattattttt cttcacgata tgcttccatt ctcgaaaaaa
     6001 aatataatat tccccttgta agggtacagc accatcatgc acatatagct tcatgcatgg
     6061 ctgaacatgg attggatgaa agagtggccg gggtatgcct cgacggcacc gggttcggaa
     6121 cagacggcca tacctgggga gctgaattcc tggtagcaga caggaaggag ttcctcagat
     6181 ttggccattt tgactatgta cctatgccgg gaggggataa ggccgttgcg gaaccctggc
     6241 ggatggcact ctcttacctg tatatgtatt ttggtgatac attgaattac agggatattg
     6301 aaccgttcag caagataagc ggtaatgaat tcatgctggt catggaaatg atccggaaga
     6361 aaatcaacac tccccttact tgcggagcag gaaggctctt cgatgctgtt tccgccctta
     6421 caggaatctg tccggaagca ggttttgact ctgaagcgcc aatgcgcctg gaatctgcaa
     6481 ttaaatgctg taccgaaaaa cattatcctt acagggtgaa cgggtctgta tcttttgggg
     6541 atatgtttga agccatattg aacgacatca tggtcagaaa tgcatccgta tccctaatct
     6601 cggcaaagtt ccacaacacc atggcacagg ttattcttga catgtccgaa cggatacggg
     6661 aagagcattc ggttgatgtt gtggttctca cgggcggagt atttcagaat aaatatcttc
     6721 tggaaagggc ttctgagaaa ctccggaaag caaatttcag ggtttatacc agtaaccagg
     6781 tgccggccaa cgacggaggg atctctctcg gacagatggc agtggcatca aaattatccg
     6841 gattatgtgc ctgagtgtgc cagcgcttgt tgtcagcgta aacggagatg tggctgaagt
     6901 ttcagttgga ggtgcccttt tcagggccgg gctgcaaatg gtggaaaatg tcagtcccgg
     6961 cgactatgtt ttgcttcatg cgggatttgc cattcagaaa atcagtgaag atgaggccat
     7021 gtcgaccctg aagcttctca gggagcttga tgatgtctca ggtcagtgag ccatgaatgc
     7081 ccccttaatt tatttcattt actggcacat gtatatataa ttcccgtaat gcggttccca
     7141 tcataccagg aggatcagga atttgattaa tatgaagtat atcgaagagt tcagggataa
     7201 ggatcttata aagaaactga tcaggcatat cgggatggtg atgcggggta aatatgtttt
     7261 catggaggta tgcggcgggc atacatctgc cattcatcgt tttggcatcc cgtcgatgct
     7321 gccttccggc atcagcctga tatccggtcc cggatgtcct gtttgcgtga ccgcaatgga
     7381 tttcatcgac agggcaatag tactggcagg gtacagtgat gtgatcatag ccacattcgg
     7441 cgacctgatc cgtgttccgg gatcctcatc gtcgcttgaa aggaagaggt cggcaggcgc
     7501 tgacgtgcgg atcatctttt caccgctcga tgcccttgat attgcaaggg agaatccggg
     7561 gaaaaaggta gtgttcgcag gtataggatt tgaaaccacg gctccggcaa ccgctgttac
     7621 tgtactgcag gccggaaaag aaaaaatcgg gaatttttat gtcctgagtg cacacaaatt
     7681 gatgccgccg gtaatggaag ctgttgccgg tggaggcact gcgctcgatg gctttat
//
LOCUS       DAOOYG010000127         7726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10627_length_7726_cov_6.900013, whole genome shotgun sequence.
ACCESSION   DAOOYG010000127 DAOOYG010000000
VERSION     DAOOYG010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10627_length_7726_cov_6.900013"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..693
                     /locus_tag="PK288_09655"
     CDS             310..693
                     /locus_tag="PK288_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19231.1"
                     /translation="MRDSRIFVDDADGLTTITNDISGYKIDHSRIVTGKIDEGEFLDA
                     IVPVYTLRGQTVMGYSHLLVINSSGSFAVVKIMNDIFRIHGISDGHVIAEVSTVDPDS
                     PGFGCNECKEVIKLTYRDGNLIREE"
     gene            complement(991..2817)
                     /locus_tag="PK288_09660"
     CDS             complement(991..2817)
                     /locus_tag="PK288_09660"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM19232.1"
                     /translation="MKKLSIFLSVLVIIAIISSCSEAFLEKTPQGKTSENVFYNEKGI
                     DALLTGTYALISGSALWYISWGASIQNWTYGSAASDDAYKGSELTDQVPVNEIERWEV
                     TTTNQYPAEKWRLCIGMGVDRANKTLNVIKKTEEAGEITADVATRFRAETRFLRGFYN
                     FEAWLVFGDYIPILLEDTPDPKLVSNKNEKGAVLEHIVSDMKYAWENLPESQADPGRP
                     TRYAAMAIAARAYLQELRYSDAKPLLDNIIHSNKYDLMPYFIDNFEIATENNKESVFE
                     IQACVNDVSESLNAEMGIGLNFPHGGDIGFCCGFHQPSQNLVNAFKVDADGLPLFDTF
                     NNTDLKNDAGIPSDQAFTIATDPVDPRLDFTVGRRGIPCKDWGINRGSNWIRKQSDGG
                     PYLPALKPFFNKKELYSLSTTTGWQTGVNANNFRYIRYSHILLWRAEVAAYEGDLVTA
                     TTYVNMIRERAGKKVVMGKVLIDVLPANVYPWGPNTTDADYMVSNSTIDWSQPAANYK
                     IGLYHPFTSKEEAMRAVQWEQRLEFATEGRRFFDLRRWDDLPAGMRVDMAATLNAFAA
                     GDLRIREFMRGANFSNKDKFMPVPQTQIDLQPGVLVQNPDYQ"
     gene            complement(2852..6130)
                     /locus_tag="PK288_09665"
     CDS             complement(2852..6130)
                     /locus_tag="PK288_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM19233.1"
                     /translation="MTKKLLSAATPIGLEFFRKGVKLLTMILMSGTILLSGANAAAIP
                     SDNLQAITVTGVVTDIEGAPLAGVNVVEKGTVNGTITATDGRFMLTVASSASVLTFSF
                     VGYSTQEIVVGNQKTINVSLEEVVSSLDEIVVVGYTTQARKNLSGSVSTVSSDQLTVS
                     TAPSAVSRLQGQASGVTITSSNVPGGEATIRIRGIGTINDPDPLYIIDGVPTEPGNNL
                     SATDIESISILKDAASAAIYGSRGANGVIIITTKRGRFNEQPNFEFNVRTGSTKAINR
                     YDMLNTQEYADAVWLMRKNNEITKAHAQYGNGATPRIPDYIWPAGKMEGDPAINPDLY
                     DYHDYPITKANKKGTNWYDEIYRSGFVQEYDMAIRGGGQNASYSFSGNYLDENGTLIH
                     TNFKRWTFRMNSDAKFNDWFKVGQSLQAVYITQHGDFGNNGEGTAVSQAYRAQPICPV
                     YDIGGNFAGSRASELGNFGNPVAQLFRARNNEGTWARALGNFYGEVTFLKGLTAKSLL
                     GYNFGQWNFNGYTIPNFEHSEANKVNGAWLEGSHALQWNWTNTINYTGTFADIHRVNV
                     VVGTEAIDSYDKWTGASRSQFFSEDPTYMQIDAGEINKDNYGNASSWSLFSYFGRLNY
                     DLRGKYFLEATFRRDGSSRFGPEHRYGNFPAASLAWSISEEGFMAGTREWLDFLKLRT
                     GWGLSGNDRIGNYNAYSTYATDKYLAAYPLDGSNTSAISGFMPSTLGNPNVGWETTQT
                     INAGIDAIILKNKLSLSFDVWQRDTKDMLYQLKIPEVMGLADPPFVNIGQMRNKGFDL
                     ELGYKNSAMNGKFSYNIRATLSRYTNKIMKLSDEVNEEIISGGLRQINYTRAAVGTAF
                     PQFYGYEVEGIFQTDAEASSYAPYGSNPYNKAGHFKFKNQLTIDSNGDGVPDEADGII
                     DPNDMVYIGNPHPDLTGGLNIDLAYGPLSLNMFFYGSYGNDMVNYVTRWIDYGMFNGG
                     LSHKALYESWGSPYLRDNAKAKLPMLDQNDISQQPSTAFVQDASFLRFKTLRIGYSLP
                     TKLVNKIQAKSLSIYAQVTNLFTLTKYEGLDPELDSSGSYMGLDQGAWPTPRQIMFGI
                     NLGL"
     gene            7494..>7726
                     /locus_tag="PK288_09670"
     CDS             7494..>7726
                     /locus_tag="PK288_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPM19234.1"
                     /translation="MNNKPALYGGIKTVEKSFPWPIYDESDIEAVAGVVRSGEWGNPD
                     CGDIVRDFEDRFAEFCGTWYALSCVNGSVVAVS"
BASE COUNT         2059 a         1767 c         1736 g         2164 t
ORIGIN      
        1 acaatctgta ttttatatgt taataactta ttctaagtca actcttaatt cgcattaata
       61 attaactccg cattactaaa tacaaataat aacatgcatg aaaacaattc aattccggta
      121 atgaacagca ccagactgtt tccggcagca aaattaaacc ggattaaacg gatatgcctg
      181 ctgttttttt gcatcgcctt tatgatgagt tgcatttcta acaattcctc agtaagacgc
      241 cgggataaaa ttgcggaaaa aacgcgtacg gaagtcattg cgtttgtaga aaagtatctg
      301 cagaaccgtt tgagagattc caggattttt gttgatgatg ccgatggctt gacaaccata
      361 accaatgata tctcaggtta taagattgat cattcccgga tagtgacagg aaagatcgac
      421 gagggtgaat ttttggatgc tattgttccg gtttatactc tccgggggca gacagtcatg
      481 ggatactcac atctcctggt gattaattca tcaggatcat ttgccgtggt aaagatcatg
      541 aatgatattt tcaggatcca cggaataagt gacggtcatg tcattgccga ggtatccaca
      601 gttgatcctg actcaccggg ctttggctgc aatgaatgca aggaagttat caaactgacc
      661 tacagggatg gaaacctcat aagggaagaa taagaaaggt gaaggtatcc cttttttcag
      721 gctttcagca agtccgataa ccaggtaaac aaatatcata acagaagcac tgcgaatatc
      781 gtgctccggg tacaaacctg taaatgtcca tgccgctccc tgtttttgaa gcggcgaccg
      841 gcgttgcaca gctggcaaaa agcaggacct attgagttat cagactcttt tcctcatgtt
      901 gctttcatgc atgagtagca gaagaaagtg aaattaacct gaaaaggaag ggtgtcccat
      961 tgagacaccc tttttcaatt cagtcaattg ctactggtaa tcgggattct ggacaagtac
     1021 gcccggctgc aggtcgatct gggtctgcgg cacaggcatg aatttatcct tgttactgaa
     1081 atttgcaccc ctcatgaatt ccctgattct gaggtcaccg gcggcgaatg cattaagtgt
     1141 ggcggccatg tcgaccctca ttccggcggg aagatcgtcc cagcgtctca ggtcaaagaa
     1201 acgacgtcct tccgttgcaa actcaagcct ttgttcccac tggacagccc tcatagcttc
     1261 ttccttgctg gtaaacggat gatagagacc aattttataa ttggcggcag gctgcgacca
     1321 gtcaatcgtt gagttggaga ccatgtaatc agcatcggta gtattaggac cccacggata
     1381 aacgtttgca ggcagcacat cgatcagcac ttttcccata acaaccttct tccctgccct
     1441 ttcgcggatc atgttaacat atgtagttgc tgtaacgagg tcgccctcgt atgcggcaac
     1501 ctcggccctc cacagcagaa tgtggctgta acggatatac ctgaagttat tggcattcac
     1561 acctgtttgc caccccgtag tggtggacaa cgagtacagt tccttcttgt tgaagaaagg
     1621 cttcagggca ggaagataag gaccgccatc cgattgcttg cgaatccagt tgctcccacg
     1681 gttgataccc cagtctttgc agggaatccc cctgcggcca acagtgaaat caagacgcgg
     1741 gtcgaccgga tcggtggcaa tggtaaaagc ctggtcggaa ggaattccgg catcattctt
     1801 caggtcagtg ttattaaagg tatcgaacag cggtaatccg tctgcatcca ctttaaatgc
     1861 atttacaaga ttttgcgaag gctggtggaa accgcagcag aaaccgatat cacctccgtg
     1921 agggaagttg aggccaatac ccatttcagc gttaagcgat tcgctgacgt cattgacgca
     1981 tgcctggatt tcaaatacag actccttatt attctcggtg gcaatttcaa aattgtcgat
     2041 aaaatacggc atcaaatcgt atttattact gtggataata ttatcgagaa gcggttttgc
     2101 atcgctgtac ctgagttcct ggagataagc cctggcagca atcgccattg ctgcatacct
     2161 ggtcggacgt ccagggtcag cctgactttc gggaagattt tcccaggcat acttcatgtc
     2221 gctcactata tgctccagaa cggcaccctt ttcattcttg tttgaaacca gctttgggtc
     2281 gggtgtatcc tcaagcaaaa taggaatata atctccaaaa accagccagg cctcaaaatt
     2341 gtaaaaaccc ctgaggaatc ttgtttcagc cctgaagcgg gtagccacgt cagcggtgat
     2401 ctcgcctgct tcctcggttt ttttgatcac gttcagggtt ttatttgcac ggtcaactcc
     2461 cattcctatg caaagccgcc acttttcggc cggatactgg ttggtggttg taacttccca
     2521 gcgttcaatc tcgttcaccg gcacctggtc ggtaagttca gaacctttat aggcatcgtc
     2581 tgatgctgct gacccatagg tccagttttg aatggacgct ccccaggaaa tataccacaa
     2641 ggctgagccg ctaatcaggg cataagtccc ggtcagcaat gcatcaatgc ctttctcatt
     2701 atagaagaca ttttctgatg ttttgccctg aggggttttt tcaaggaatg cctccgagca
     2761 ggaagagatg attgctataa taaccaggac acttaaaaat atcgatagtt ttttcatatt
     2821 tccagttgtt ttaattttca attatgatat ctcacaaacc caggtttatg ccaaacatta
     2881 tctggcgtgg tgtcggccat gcaccctggt cgagtcccat atatgatccg gatgagtcca
     2941 gttcaggatc aagcccctca tacttcgtca gggtgaacag gttagttacc tgtgcataaa
     3001 tgcttaaact ttttgcctga attttgttaa ccagcttcgt cggtaatgaa tagccgatcc
     3061 ggagggtttt gaatctcagg aatgaagcat cttgtacaaa cgcggttgaa ggctgctggg
     3121 aaatatcgtt ctggtcaagc atcgggagct tggccttggc attgtcacgc aggtacgggc
     3181 tgccccagga ctcatacaaa gccttgtggc tcaatccacc gttgaacatt ccataatcga
     3241 tccagcgggt tacataattg accatatcgt tcccatagct tccatagaaa aacatattca
     3301 gacttagcgg accataggca aggtcgatgt tcaggcctcc tgtaaggtcg ggatgcgggt
     3361 ttccgatgta aaccatgtca ttgggatcaa tgatcccgtc agcttcatca ggcacaccat
     3421 caccgttcga atcgattgta agctggtttt taaacttgaa atgccctgcc ttattgtagg
     3481 gattggaacc atacggggca taactgctgg cttctgcatc ggtctggaat atcccttcaa
     3541 cttcataccc gtaaaactgg ggaaatgcag ttccgacagc agcccgggtg taattaatct
     3601 gacgcagccc tcctgaaata atttcttcgt tcacctcatc agaaagtttc atgatcttat
     3661 tggtataacg ggaaagggtt gccctgatgt tgtacgaaaa ttttccgttc attgctgaat
     3721 ttttatagcc cagctcaagg tcaaacccct tgtttcgcat ttgaccgata ttcacaaatg
     3781 ggggatcagc aagtcccatt acttccggaa tcttgagttg gtaaagcatg tcttttgtat
     3841 ctctctgcca aacatcgaaa gataagctca acttgttttt aagaattatg gcatctatac
     3901 cggcgttgat tgtctgggtg gtttcccatc ccacgttcgg gttgccgagt gttgaaggca
     3961 taaaaccgct tatggcagat gtatttgagc cgtcaagcgg gtaggctgca aggtacttgt
     4021 cagttgcgta agtagaatat gcattgtaat tgcctatcct gtcattcccg gaaagacccc
     4081 accctgttct taacttcaga aagtcgagcc attcacgtgt accggccata aagccctctt
     4141 cggatattga ccatgcaaga gaagcagccg ggaaattccc gtagcggtgt tccgggccga
     4201 agcgtgaaga gccgtcgcgg cggaaagtag cttcgagaaa atacttgccc cggagatcat
     4261 aattgagacg cccgaaataa gaaaaaagcg accacgatga agcattgcca taattatcct
     4321 tatttatttc tcctgcatcg atttgcatgt aagttggatc ttcggagaaa aactggctcc
     4381 tgctggctcc tgtccattta tcatagctgt cgattgcttc ggtccctaca acgacattga
     4441 ccctgtgaat atcggcaaat gttcctgtgt agttaattgt gttcgtccag ttccactgca
     4501 gggcatgtga gccttccagc catgcgccgt taaccttgtt ggcctcggaa tgttcgaaat
     4561 tggggatcgt gtaaccgtta aaattccact gtccgaaatt atacccgagc agactctttg
     4621 cggtgagccc tttcagaaaa gttacttcac cgtagaaatt accaagggcc ctggcccatg
     4681 tgccctcatt attccttgcc ctgaaaagct gggcaacggg gtttccgaag ttccccagct
     4741 ccgaggccct tgaacctgca aagttgccgc caatgtcgta cacagggcag atcggctgtg
     4801 cgcggtaagc ctgggataca gctgttccct cgccgttatt gccgaaatcg ccgtgctggg
     4861 taatgtaaac ggcctgaagc gactgaccta ccttgaacca gtcattgaat ttagcgtcgg
     4921 aattcattcg gaatgtccac cttttaaaat tggtatggat cagtgttccg ttctcatcca
     4981 ggtagttgcc ggaaaaagaa tagctggcat tctggccccc tcccctgatt gccatgtcat
     5041 attcctggac aaaacctgat cggtaaatct cgtcatacca gttggtccct tttttatttg
     5101 ccttcgttat cgggtaatca tggtagtcgt aaaggtcggg gttaatagcg ggatctcctt
     5161 ccatttttcc ggccggccag atataatcag ggatcctggg agtagcacca ttcccgtact
     5221 gggcatgtgc tttggtaatt tcgttattct tcctcataag ccaaactgca tcggcatact
     5281 cctgtgtgtt gagcatatcg taacggttta ttgccttagt ggatcccgtc cgtacgttga
     5341 attcaaaatt cggctgttcg ttgaaacgtc ctctttttgt tgtaataatg ataactccgt
     5401 tggctcccct cgatccatag attgcagccg aagcagcgtc cttaaggatg gatatggatt
     5461 caatgtcggt agcactgagg ttattacccg gttcggtagg cactccgtca atgatgtaca
     5521 acggatccgg gtcatttatg gttccgatcc cgcggatacg gatggttgct tcaccgcccg
     5581 gaacatttga ggaagtaatt gttacgcctg atgcctgacc ctgaaggcgg ctgactgcag
     5641 acggagcagt ggaaaccgtc agctgatcag acgatacggt tgacactgaa ccggaaaggt
     5701 ttttccttgc ctgcgtagtg taaccaacaa caactatttc atcaagtgat gaaaccacct
     5761 cttcgaggct tacattgatc gttttctggt taccgacgac aatttcctgt gttgaatatc
     5821 cgacgaagga aaaagtaaga acggaggctg aagaggcaac tgtcagcata aacctgccat
     5881 cggtagctgt tattgtacca ttgacagtcc ctttttcaac aacgtttact cccgcgagag
     5941 gtgcgccttc aatatcagtg accacacctg taacagttat ggcctgcagg ttgtctgacg
     6001 ggatggccgc ggcattagcg cctgatagaa ggatcgtccc ggacattaga atcattgtca
     6061 gtagttttac gcccttcctg aagaattcca ggccaatggg cgttgctgca gatagaagtt
     6121 tctttgtcat aaggttagta gatgagtgtt taattagaat tttagggaaa tttgttaaaa
     6181 accaggcaag atcactttta attaactgag atcctgttgg ttaaaagtca gtctcaacgg
     6241 gtaatctgtg cccgttgctc atttagatag gatgtgatga tttcatgatt ttaaatttag
     6301 gttagctttt agaaatatga agcgctaata aaataacgcc gatggaatta ccagaattag
     6361 ccacgagccg gcgggccgtg atataaacaa tacccgatga tattgagatt accggaatat
     6421 attccgatac cggacagcgc tttgaaaata gatatgacgc ccagaatttt acaaacaggg
     6481 aataactctt ccacgtcagg ttttattaaa aaaatacccg accctaaagc taatttgctt
     6541 tccggaattc gaattacaat cttctgtgtc ctgaagatcg acatttgatt tcattaaggt
     6601 ttgaccgaga ttctaaaacc agcaaaagta agccgcttat ctgcgatcaa cgcatcttaa
     6661 aaatccgaac cgacaaacac atctgaaaaa caagcctgaa tcgtgattat atttccaaac
     6721 catggtctta ggacaaccaa atataaaaaa aaattcctgc aaatccaaca ttatgaaaat
     6781 attttacata cagatttaca tgttatgatg gccattcagc acaagattcc cggctcccgt
     6841 tatctcaaac cccgattaaa ccatcgggaa aaatcaatta attcagtcag aacttcaatg
     6901 gatctacact gtaaggacga cctgacatgt agtatattcc tccgggaccg gagttattac
     6961 ctaaattccg ggcttattat tattaacaca taaatttctt cattcatgcc agatatcctc
     7021 cggcacagca gtcaattcac cggctgagga atcctttgtc agacactcag atgcatgtca
     7081 ttctgtctcc tgtcagcaaa tacatttatt ttcaggctgc ggatctgtgt ccggagttga
     7141 tatgaaaatc aatgatagtc caatttaatc agccgtaacg ggtatggagg aaatggcatt
     7201 aaactgcatg cccatgttat ggaaaaggta tttaggatta tataagctgg ctcagtgata
     7261 ggcatctcac tgagtcagga attagttccg gcctggacag atatggttac ctcccctttt
     7321 aaattcagga atattattaa tatctttact cagcatcatt actgttacaa ttttgcatta
     7381 gactgttaac atattaacac tcaatatatt aatcaatata gcaaattgtt ttgttctata
     7441 aattcctatc tgaccttcaa tattctggta aatcataata ctttaataac agtatgaata
     7501 ataaacctgc tctttacggg ggaatcaaaa cagttgagaa atcattccca tggcccatat
     7561 atgacgagtc tgatattgaa gcggtagccg gagttgtacg gagcggcgaa tggggcaacc
     7621 ccgactgcgg tgatattgtc agggatttcg aggacaggtt tgctgaattc tgcggtacct
     7681 ggtatgctct cagctgtgtc aacggctcgg tggtcgccgt atcatt
//
LOCUS       DAOOYG010000128         7709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10670_length_7709_cov_7.489548, whole genome shotgun sequence.
ACCESSION   DAOOYG010000128 DAOOYG010000000
VERSION     DAOOYG010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10670_length_7709_cov_7.489548"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            592..1182
                     /locus_tag="PK288_09675"
     CDS             592..1182
                     /locus_tag="PK288_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19235.1"
                     /translation="MKNKVLFGFATILMVAFLAGCGKVPQVEIDAAKAAIEAAKAAEA
                     DVYIPVELAAVQDSMNAVLANVEAQKSKLFKKFGPAKEKLAAVVAMANQAAANAAVKK
                     EEVRKESEKLLNDIKAVTEENGKLILKAPRGKEGAAVIEQIKADMAAIDATVAEAQDF
                     FNKGAFMNALNKIKAAKEKADGINTELKEAIAKVRR"
     gene            1251..2366
                     /locus_tag="PK288_09680"
     CDS             1251..2366
                     /locus_tag="PK288_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPM19236.1"
                     /translation="MGRKKVLSYIFVPVIIVLGCAVLALLLAYLAPRPPVEKVENARI
                     TLSRALTFSANIYSADLYRESERFYDSSMVKWSRENERFFLFRNFDEVEKYADLSVEK
                     AKEAIETAKENVISIKTRIEYRIDTLNNIVAHLNKIFTNYPLSTEVREMISKGKLLLK
                     EAEIAFSREEYLHANTKIVDAEHFLTESLNNATGNLEDYFRSYPIWRQWVDKTISDSR
                     RNKSYSIIVDKYARKCHIYYNGIRQHVYDVELGKNWVGHKRIKGDKATPEGMYKVIRK
                     LGSGRTKYYKALLLDYPNEEDKAEFRRDLETGVLPPTAGIGGLIEIHGRGGRGIDWTD
                     GCIALTDWEMDVVFRLAGEGTPVTIVGSMVKLDEIHN"
     gene            2502..3194
                     /locus_tag="PK288_09685"
     CDS             2502..3194
                     /locus_tag="PK288_09685"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPM19237.1"
                     /translation="MHENKKLLGVIRTTLISVVTLLSVVFLVFYILFAVPQFQELPYG
                     KGSGPANLKELKEDKNYRKITGTLSKDVQRLLRKYNSYTSGQSYIVINTTYNKFHLFR
                     NKKLVREGRCSTGSYIMLKTHGEKKWIFKTPKGKYWIQGKIASPVWRKPDWAFVEEGL
                     PIPPPNHPSRWEPGVLGDYALSIGDGYLIHGTIYKRFLGMPVTHGCVRLNDEDLEVIY
                     KTLNIGSKVYIF"
     gene            3214..4011
                     /locus_tag="PK288_09690"
     CDS             3214..4011
                     /locus_tag="PK288_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19238.1"
                     /translation="MKTETGATKTGGKLTIIVIAVLVILFLVYYSVMAMLAPLKKLNE
                     LKEKYTPEYEEAASGSRILTDSTYLSLIKEKAFLQSKINMAETDSIYLVLNLADSIAS
                     LEISGVEVHSARISFFRMSSILGKGNEYLMYSLLSGPLNIVGDYSTIRKEPLMIKMAP
                     RDTSEYKPDIIPDTTYHEPVNYILETDIGIRIFIYQSEKILPSDRKHQYFFDLNDRLR
                     NTWNSLKRVAILEVPEYHPFIKLVLPGADARIIYRAIPRKGQIAVYL"
     gene            4092..5369
                     /locus_tag="PK288_09695"
     CDS             4092..5369
                     /locus_tag="PK288_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HPM19239.1"
                     /translation="MARFTGIIGIIIILGLAFLWSNNRKAINKRLVITGLLLQLAIAI
                     FILKVPLGQDIFAFLGRAINKLLDFSQEGGVFVFGDLTKISQILPPGIVSAGVFLFIL
                     IPTIIFVCVLVAMAYHVGLMQRIVALIARFVHWAMRVSGSEALSNVASAFVGQVEAQI
                     MIRPYLSGMTMSELLASMTGSMACIAGGVMAVYISMGVPASYLLAASLMAAPGALVIS
                     KIVFPETQESETKGTVKLEVKKQYTNIMDAISHGATDGMTISINVIAMLIGVIALVAL
                     ADALLGYFGLFLGWTGISLDFIGLDVTHLRLKDVFGAFFSLFAVVMGVPASESFTVGS
                     LMGTKMVINEFVAYSDLSPLIRQGALSAKSVIIASFALCGFANFSSIAIQLGGIGAMV
                     PNRKPDLARLGFKAMICGTMASYVSASLAGILM"
     gene            complement(5688..5840)
                     /locus_tag="PK288_09700"
     CDS             complement(5688..5840)
                     /locus_tag="PK288_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19240.1"
                     /translation="MREQRAWQHLAFAGSPIMDHHLSASRQTGGNPRRRITDELQAGS
                     EAVILN"
     gene            complement(5946..6034)
                     /locus_tag="PK288_09705"
     tRNA            complement(5946..6034)
                     /locus_tag="PK288_09705"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5998..6000),aa:Ser,seq:cga)
     gene            6339..>7709
                     /locus_tag="PK288_09710"
     CDS             6339..>7709
                     /locus_tag="PK288_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPM19241.1"
                     /translation="MKTFYKMLFAGGLFAVMLTACRNQTKEDMANPFFSDYDTPYNVP
                     PFEKIMARHYMPAFEKGMEEGRMDIRRLINNREEPNFENTIGALDQAGELLTKVSAVF
                     FAQTSANTNDSLQKIEMEISPRLAGYNDEIILNPDLFKRIKKVYDNRDNQKLTSEQRF
                     ILENLYREFVRNGANLNKQDQDTLKKINQRISVLSVKFSQNVLEETNNYKLFVNRDGL
                     AGLPQPLIDAAAEAATAAGREGEWAFTTHRPSIFPFLQYSENRDLRRQLFNAYTNRGN
                     NGNENDNNLVLAEIIKLRARRARLLGYKSHSDLVLEPRMAKNPANVFNLLNNLWSKAV
                     PVAMNEVKEMQKIIDREGGKFRLEPSDWWYYAEKLRKEKYALDDNALRPYFRLENVRE
                     GVFTCANRLYGITFEPIHDCPLPHPEAQAYEVREEDGTHIGVLYMDFHPRESKRQGAW
                     CGGYRNH"
BASE COUNT         2240 a         1558 c         1887 g         2024 t
ORIGIN      
        1 cgggatcacc gacgagctgc cggcaggcag tgaggaggta atccccgaag aaagcctgct
       61 ttctgaaggg atggaggtaa aataacaaaa acccgcaggg tttatgcagg ctttgcaggg
      121 agccgccctc cgggatcacc gacgagctgc cggcaggcag tgaggaggta atccccgaag
      181 aaagcctgct ttctgaagga ataaaggtaa aataacaaaa acccgcaggg tttatgcagg
      241 ttttcaggga tctgtcccga taattacctg gctggcttgc agccggaatc gatcagggga
      301 ctacgggttt tagaaactta ttaaaagtat atgcctggcg atcttcctta agtaaataat
      361 actctgcaag tggatcgaag tgccctggat attcgccggg gttccggtac cattgtacat
      421 gcatttttgt tctgatatat aagaacagtt tcgtgacacc caggaaatta attttttatt
      481 tttttgttgc atgttcgtta aaatgaattt aattttgcac cggttttttc tgataggaag
      541 attggaaata accaaaaaat taacgaaaca aagtaactaa aaggaagcaa aatgaagaac
      601 aaggtgttat ttggatttgc aactatttta atggttgcat ttcttgcagg ttgtggcaaa
      661 gtgccgcagg tcgaaattga tgcagcaaag gctgcaattg aggctgccaa ggcagcagaa
      721 gcagacgttt acattccggt tgagcttgca gcagtgcagg attcgatgaa tgcagtactt
      781 gccaatgtag aagctcagaa atcaaaactt ttcaagaaat ttggtccggc caaagaaaaa
      841 cttgctgctg ttgttgctat ggcaaatcag gccgcagcca atgccgcagt aaagaaagag
      901 gaagtcagga aagaaagtga aaaactgctt aatgacatta aggctgtaac agaggaaaac
      961 ggcaagctta tccttaaagc ccccaggggc aaagaaggag cagcagtaat tgaacagata
     1021 aaagccgaca tggctgcaat tgatgccaca gttgccgagg ctcaggactt tttcaacaag
     1081 ggtgccttca tgaatgcact gaacaagatc aaagctgcaa aggaaaaggc agatgggatc
     1141 aatacagaac tcaaggaagc cattgcaaaa gtcagaagat agtcagagct tttacagcta
     1201 aagctaaaat ccccgggaat gaacccgggg ttttttttaa ggtcagggga atgggccgga
     1261 aaaaagtttt gtcatatata ttcgtgcctg tcattattgt gcttggttgt gccgtgctcg
     1321 ctttattatt ggcttatctg gcaccccgtc ctcccgtgga aaaagttgaa aatgccagaa
     1381 tcactctttc ccgggctttg acatttagtg caaatatata ttcggcggat ctttacaggg
     1441 agtctgaacg tttctatgac tcttcgatgg tcaaatggag cagggaaaac gagcgatttt
     1501 tccttttcag gaatttcgat gaggttgaaa agtatgcaga tctgtcggtt gagaaggcaa
     1561 aggaagcaat tgaaactgca aaggaaaatg tcatttcaat caaaacgaga attgagtaca
     1621 ggattgatac tttaaacaac atcgtagcgc atttgaataa gattttcaca aactatcctc
     1681 tctccacgga agtcagggaa atgatatcca agggtaaact attgctgaaa gaagctgaaa
     1741 tagctttcag cagggaagaa taccttcatg ctaatacaaa aatagttgat gctgagcatt
     1801 ttctgactga gtctcttaat aatgcaacag gtaatcttga agattacttc agatcatatc
     1861 cgatatggcg gcaatgggtc gacaaaacca taagcgattc gagaaggaat aaaagctatt
     1921 cgattatagt ggataagtat gccaggaagt gccatatcta ttataacggg atccgtcagc
     1981 atgtttatga tgtggagctt ggaaaaaact gggtggggca taagaggata aagggagaca
     2041 aagccacacc ggaaggaatg tacaaggtta tcaggaagct gggatccggc aggaccaaat
     2101 actacaaggc attgctgctt gattatccaa atgaggaaga caaagctgaa ttcaggcgtg
     2161 atctggaaac gggtgtactg cctccaacgg caggaatagg aggtcttatt gaaattcacg
     2221 gcaggggagg aaggggaatc gactggaccg acggatgcat tgcgttgact gattgggaaa
     2281 tggatgtcgt ttttcgcctt gcgggcgaag gtaccccggt aaccattgtc ggatcgatgg
     2341 ttaaacttga tgagattcac aattgaaatt attttaacca tatggaagag gacagggtac
     2401 agaaggaagg gaatgaatcc ggatttccac cagggaataa aatgactttg catgaaataa
     2461 gtaaacgggt atcagcgcat atcagccggt tttacgcggg agtgcacgaa aataaaaagc
     2521 tcctgggtgt cattcgtaca acactcattt ctgttgtaac cctcttgtca gtggtcttcc
     2581 tggtatttta tattctattt gctgtaccgc aattccagga attaccctat ggtaaaggat
     2641 cgggaccggc gaatctgaaa gaattaaagg aggataagaa ttacagaaaa attacaggaa
     2701 ccttgtcgaa agatgtgcaa aggcttctga gaaaatataa ttcatacaca tcaggccagt
     2761 catatattgt aatcaatacc acttacaaca aatttcatct tttcagaaac aaaaagcttg
     2821 tcagggaagg ccgctgcagt acgggaagct atattatgct gaagacccac ggagaaaaga
     2881 agtggatctt caaaacaccc aagggcaaat attggataca gggaaagata gcttcgcctg
     2941 tgtggagaaa acccgactgg gcttttgttg aagaaggttt gcccatcccg cctccgaatc
     3001 atccttcacg atgggaaccg ggagtgctgg gtgattatgc tctcagcatt ggtgacggat
     3061 acctgattca cggaaccata tataaaagat ttctcggaat gcctgttacc catggttgtg
     3121 tcaggctgaa cgatgaagat cttgaagtga tatacaaaac attgaatata ggctcaaaag
     3181 tttacatttt ctgaatcatc tcaagttcta cccatgaaaa cagaaacagg agccacgaaa
     3241 accggaggaa aactgacgat aattgttatt gcagtattgg tcatactgtt tctggtttac
     3301 tattcggtta tggcaatgct tgctccgctg aagaaactca atgagctgaa ggaaaaatat
     3361 acaccagaat atgaggaagc cgcttccggc agccggatac tgactgattc cacctattta
     3421 agcctcataa aggagaaggc attcctgcag tcgaaaatca atatggctga aactgactcc
     3481 atctatctgg tgctgaatct ggcagacagt atcgcaagtc ttgaaataag cggtgttgag
     3541 gttcattcag caaggatcag ctttttcaga atgagctcaa tccttggaaa aggaaatgaa
     3601 tatctgatgt attcattgct gtcgggtccc ttaaatattg tcggggatta ttcgacaata
     3661 aggaaagaac cacttatgat taaaatggcc cccagggaca cctctgaata taaacctgac
     3721 atcattcctg acaccaccta tcatgagcct gtgaattata tcctggaaac cgatattggc
     3781 atcaggattt ttatttatca gtctgagaag atattaccat ccgacagaaa acatcagtac
     3841 ttttttgatc tgaatgacag gctcaggaat acatggaatt cccttaagcg tgttgcgatt
     3901 ctggaggttc ctgagtatca cccgtttatt aagctggttc tgcccggggc cgatgcaagg
     3961 atcatataca gggccatccc gagaaagggc cagatagcag tatatctgtg acatcttttt
     4021 gcagtaatgc aatacatcgg atcgaaaaaa aaagtaggtt tgagccatgc taaaaactaa
     4081 ttcattctta catggcaagg tttaccggaa ttatcgggat aatcattatt ctcggactgg
     4141 catttttatg gtcgaataac cggaaggcaa tcaataagcg cctggtgatt accggcctgc
     4201 tgctccagct agccattgca atatttattc tgaaagtgcc attgggtcag gacatttttg
     4261 ctttccttgg cagggcaatc aataaacttc tcgatttttc gcaggagggt ggggtgtttg
     4321 ttttcggaga tctcacgaag atatcacaaa tattacctcc gggaatcgta agcgccggtg
     4381 ttttcctgtt catcctgatc ccaacaataa tatttgtatg cgtacttgtt gcgatggcct
     4441 atcatgtagg tttgatgcaa aggattgttg cgctgattgc ccgctttgtg cattgggcga
     4501 tgcgggtaag tggcagtgaa gcgctgtcga atgttgcaag cgcctttgtg ggtcaggtgg
     4561 aagcacagat aatgattcgg ccatatctgt ccggaatgac gatgtcggaa cttcttgctt
     4621 cgatgactgg gagcatggca tgtattgcag gcggggtaat ggccgtttat atttctatgg
     4681 gggttccggc ttcttaccta ctggcagcaa gtcttatggc ggcacccggg gcattggtga
     4741 tatctaaaat tgtatttccc gagactcagg aatcagaaac caaagggaca gtgaaactgg
     4801 aggtgaaaaa gcaatacaca aatataatgg atgccatatc tcatggggca acagacggaa
     4861 tgaccatctc aataaatgtg atagcaatgc tcataggcgt gattgcactt gtagcacttg
     4921 ctgatgcact tcttggatat tttggattat tccttggctg gacgggaatc tctctcgatt
     4981 ttataggcct tgacgttacc catctcaggc ttaaagatgt atttggtgct ttcttttctt
     5041 tgtttgccgt tgttatgggt gtgccggctt cagaatcttt cacggtgggc tcattgatgg
     5101 gtacaaaaat ggtgatcaac gaatttgttg cctattccga tctgtcacct cttatacgtc
     5161 agggagcttt gagtgcaaaa tcagttatca tagcaagctt tgcattatgc ggttttgcca
     5221 acttcagctc gatagccatt cagcttggcg ggataggagc aatggtacca aaccggaaac
     5281 cggaccttgc acgcctggga ttcaaggcaa tgatttgtgg tacaatggct tcatatgttt
     5341 ctgcatctct tgcggggata ctgatgtagt gatccttgca aattctgcat aaatcctccg
     5401 gggattaccg cctcactgcc tgcctgcagt tcgtcggtga tccctgatgg ggctccctgc
     5461 aaatgcaaga tgctgccatg ctcgctgctc tcgcatggat ttttatttta cattcgttct
     5521 cgctcatgca aagcatggct cggccggctg taaaataaaa atcccttccg cctgacgcgg
     5581 aagggcagca ttttggattt gcggagagag ggggggccga gcgttaagaa aacacccggt
     5641 gggtgttttt agtgaaggag ccaggttgca gggaaggcaa cccccggtca atttaggatt
     5701 accgcctcac tgcctgcctg cagttcgtcg gtgatccttc tccggggatt accacctgtc
     5761 tgcctgcttg cagacaggtg gtgatccatg atgggactcc ctgcaaatgc aagatgctgc
     5821 catgctcgct gctctcgcat ggatttttat tttacattcg ttctcgctca tgcaaagcat
     5881 ggctcggccg gctgtaaaat aaaaatccct tccgcctgac gcggaagggc agcattttgg
     5941 atttgcggag agagggggat tcgaaccccc gttccgccga taggcggaaa acggttttcg
     6001 agaccgccgc attcgaccac tctgccatct ctccattgat tgccctgatc gccatgaagt
     6061 ttgccatgct accacgcgat tgtttgttat tcggttgcct gttaatgtat ccagacttca
     6121 ccccgtgacc ggaacatcaa actggtccgg ttcaccaggt tttttacaca cttcccgggc
     6181 attgccttcc tgcggttgcg gcaaaaataa taattaaaca atatgaaaaa ccctgtaacc
     6241 cgataaaaaa ataacccgct tttgttttga ttacatttct gaatgacctt attttgcaca
     6301 accatgcagt ttctaattaa cctcttaaaa accttaacgt gaaaactttt tacaaaatgt
     6361 tatttgccgg cggattgttt gcagtcatgt taaccgcctg caggaaccaa acgaaggaag
     6421 atatggccaa tccgtttttc tctgattacg acactcctta caacgtccct ccttttgaga
     6481 aaatcatggc ccgacattac atgcctgctt tcgagaaggg aatggaagag ggaagaatgg
     6541 atatcagacg gcttattaat aacagggagg aaccgaattt tgaaaataca atcggcgctc
     6601 tcgatcaggc aggagagctg cttactaaag tttcagcggt attttttgcc cagacttcag
     6661 cgaataccaa tgattcattg cagaaaatag agatggaaat atcgccgagg ctggcaggct
     6721 acaatgatga aataatatta aatcccgacc ttttcaaaag gataaaaaag gtatatgata
     6781 atcgtgataa tcagaaactc acttccgaac agcgatttat acttgagaac ctttacaggg
     6841 aatttgtgag gaacggggcc aatcttaaca aacaggatca ggatacactg aaaaagataa
     6901 accagaggat ttctgtttta agtgtaaagt tcagccagaa tgttcttgag gaaacgaaca
     6961 attacaagtt atttgttaac agggatggtc ttgcaggact tccgcaaccg ttaattgatg
     7021 cggctgcaga agcagccact gcggcaggca gggaagggga gtgggccttt acaacccaca
     7081 ggccaagcat tttcccattc ctgcagtatt cagaaaacag ggatctgcgg cgtcaattgt
     7141 tcaatgccta cacaaaccgg ggaaacaatg gaaatgagaa tgataataac ctggtgctgg
     7201 ctgaaataat taaactgaga gcccggagag ccaggctgct gggctataaa tcacattctg
     7261 acctggttct tgagccaagg atggccaaaa atcccgcaaa tgtgttcaat ctgcttaata
     7321 atctctggag caaggcggtg cctgttgcaa tgaatgaagt aaaagaaatg cagaagataa
     7381 tcgatcggga agggggtaag ttcaggctgg aaccttcaga ttggtggtat tatgctgaaa
     7441 aactgaggaa agagaaatat gctctggatg acaatgccct caggccgtat ttcagacttg
     7501 aaaatgtgcg tgaaggagtt tttacctgtg caaacaggct ttatggaata acctttgaac
     7561 cgattcatga ttgtcctctt cctcatcctg aagctcaggc ttatgaggtc agggaagagg
     7621 atggcacgca tatcggtgtg ctttacatgg acttccatcc ccgtgaaagc aaacgacagg
     7681 gagcatggtg cggcggctac aggaaccat
//
LOCUS       DAOOYG010000129         7690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10716_length_7690_cov_6.938572, whole genome shotgun sequence.
ACCESSION   DAOOYG010000129 DAOOYG010000000
VERSION     DAOOYG010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7690
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10716_length_7690_cov_6.938572"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PK288_09715"
     CDS             <1..481
                     /locus_tag="PK288_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPM19242.1"
                     /translation="TFKVGGNYAASLRAGMDAKEKGYASTIFLDAKEKKYIDECGPAN
                     FFGIRDNTYVTPKSESILKSITNMSLIELAQNLGIKTERRQIPVEELAEFSEAGACGT
                     AAVISPIGKIFDPERNITYEYCRDGNPGPVTLKLYNKLTAIQFGDADDEFGWISVVE"
     gene            491..1369
                     /locus_tag="PK288_09720"
     CDS             491..1369
                     /locus_tag="PK288_09720"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPM19243.1"
                     /translation="MKKKESGLARAKLRGSYLTLVISVSLVLFLLGILGLVLINAKEL
                     SDYFRESLSFSVMLDDEAREADIRMLQKDLDAKPYVKSTVYVSKDEAAAKMKEDLGED
                     FISFLGDNPLPPTIDVYLFAGYTSPDSVARIEKYVLEYPFVKEIYYEESLLFLINENV
                     RKVSLFLLVISAFLFLIAVTIINNTIRLAIYSKRFLIRTMELVGATRSFIRKPFLVRS
                     VYHGIIAALIAMTLLMGLLYLIEKKFYMMFYFHSIRLLVILCVALVILGILINLVSTF
                     LSVDRYLDISEDKLYN"
     gene            1382..1639
                     /locus_tag="PK288_09725"
     CDS             1382..1639
                     /locus_tag="PK288_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPM19244.1"
                     /translation="MALKTDDKEKQNFALGRENYKLLAIGFVIIVAGFLLMLGGRSED
                     PNVFDERIFSFRRITLAPIIVLAGFVFEIWAIMKKPKDPAK"
     gene            1661..2452
                     /locus_tag="PK288_09730"
     CDS             1661..2452
                     /locus_tag="PK288_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPM19245.1"
                     /translation="MNLLEAIILGLIQGLTEFLPVSSSGHLELGKYLFGIDHESNFYF
                     SVAVHGATVLSTVFVLWKELLGLFRGVMKFTMNEETKYALKLLISMIPVGIVGLFMRD
                     TVKEYFTGNMTMLGSFFLITALLLFMTRVIKPKERDLTYIDSFIIGIAQAFAVLPAIS
                     RSGATIATGMMLGNRKSEIARFSFLMVLVPVIGANILEIGSGDLLNEGTSFAVISAGF
                     ITAFISGYIACRWMINLVRRGNLAWFGLYCVAAGIFSFILGMNGL"
     gene            2442..3152
                     /gene="truB"
                     /locus_tag="PK288_09735"
     CDS             2442..3152
                     /gene="truB"
                     /locus_tag="PK288_09735"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPM19246.1"
                     /translation="MDYEKVSAVEEGMVFLFDKPLYWTAFDLVNKVRIMIRSILGLKN
                     VKAGHAGTLDPLASGLMIICTGKATRKIEQYRDLDKEYIAELCLGASTPSFDLETEII
                     ERFRVDHITEAFVRDKLEEFIGEQDQIPPMHSAKMIEGKRAYEYARKGLKKELAPVKV
                     IFRELELLSFSIPIAKIRLVCSKGTYVRAFARDLGMALESGAYLSSLVRTSIGEFKLE
                     SAYSLESFKNFLEDLKQT"
     gene            3204..4253
                     /gene="queA"
                     /locus_tag="PK288_09740"
     CDS             3204..4253
                     /gene="queA"
                     /locus_tag="PK288_09740"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPM19247.1"
                     /translation="MKLSQFRYTLPPELIALYPAGNRDESRLLVVNRKTGALEHRVFK
                     EITEYFNEHDVLVFNNTKVFPARLYGNKEKTGAEIEVFLLRELNRDQRLWDVLVDPAR
                     KIRIGNKLYFGEDDLLVAEVIDNTTSRGRTLRFLFDGTYEEFKQTLYSLGETPLPKFI
                     NRPVEPEDSERYQTIFAKHEGAVAAPTAGLHFSRELLKKLEIIGVEFAEITLHVGLGN
                     FRSVDVEDLTKHKVDSERIRITEEAADIVNKAKDNKYKICAVGTTVVRTLESSVSTNG
                     YLKPFDGWTNKFIFPPYEFKVPDMFISNFHLPYSTLLMMVAAFAGYDLLFEAYKTAVK
                     EKYRFGTYGDAMLVI"
     gene            4699..>7690
                     /locus_tag="PK288_09745"
     CDS             4699..>7690
                     /locus_tag="PK288_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HPM19248.1"
                     /translation="MSSVNDAYSQIPWQAGYAYCKKITIQASQIEISGFGPHNNFPVL
                     IDITDNDLRTCSYGGYMENASGYDIRFTTYLGIRLYHDIEGYDPISGRLTAWVRIPSL
                     SKINTDIFLFFGNPSISSNSSSSATWSSNYKAVWHFNNSIANASAFPLVSSDFGSTST
                     TGKILEGRNFIRTSHQYISVNYNDALDFNDAITVSAWINLVTNDIDQKIFSNQNNISG
                     GYKLGIYTDGKVEFEIRTSDNHPYLNRSAPDGTILSTSTWYYVVGVYSNSEDYIRTYV
                     NGGLDRPMVTDKTAGLTGSGNPAIGRETWNDPSTFDGKFNGIIDELRVQNIARDAGWI
                     KTEFNNQSSPSTFYTVGPTASNPVAVCKPVTLYLDASGNASLTVPMVDNGSSSYCGLV
                     KTLNKYSFSCSNLGENIVTLTVADPFNTVTCNSTVLVRDAIPPVISNCPADISVSADA
                     GSCGARVNWTEPTVSDNCTPTVNIVWIKSKLPGNFFPIGTTQVNYIARDASGNTSTCS
                     FTVTVNDNTMPLINCKVSGQQNVPANTNCKYVHSGTGWDATASDNCTVSSLAYTLTGA
                     TSGSGSSLDNVVFNIGLTTVNWEATDAAGNKSQCSFTVNVTDNIIPVVICPPDITHDS
                     DPGMCSYTFTPAQATGTDNCGIDYVTGTRSDGLLLSDPYPVGVTTITWTSTDLNGNTS
                     LPCYQKITVLDKEKPVFAAPPAVTVCRSSDCSFDSNIIPAITGNVFGESDNCTPQALL
                     EIEHSDDLSGITDCNTAGTILRTWKVTDEAGNFTEKVQAIVVEPLPTVTVGNDVSSIC
                     TGGNADIVINSSTVNSIPSDFSIDVKVTSTDPGNLSGTAPLADFVLAKSQLPYTINGT
                     LINNSGNPIEVTFRFTPKIAGCNDGSTVSTEVTVHPDPAWGAIVVTPGNICLEGEVTF
                     SAELNGSAGGSVEWIRSETSGGAGIVVHSPDIPLSPGTYYYRPHYTGGYGGCNLSDGT
                     ETMVTVHPDPSWGTINTPQPDICVG"
BASE COUNT         2203 a         1643 c         1787 g         2057 t
ORIGIN      
        1 tacttttaag gttggaggca actatgcggc aagccttaga gcaggtatgg acgcaaagga
       61 aaaaggatat gcaagtacaa tatttcttga tgcaaaagaa aaaaaatata ttgacgaatg
      121 cggccctgct aatttttttg gaatcaggga caatacatac gttaccccga agtcagaatc
      181 aattctgaag tcaataacaa acatgagcct gattgagctg gcccagaacc tcggaataaa
      241 gactgaacgg agacagatac ccgttgaaga actggcagaa ttttccgaag caggggcctg
      301 cggtactgcc gccgtgatca gtcctattgg taagattttt gaccccgaaa ggaatattac
      361 ctatgaatat tgcagggatg gtaatcccgg accggttaca ctgaagcttt acaataaact
      421 taccgcgatc cagttcggag atgcagatga tgagtttggt tggatttctg ttgttgaata
      481 gggagcagtg gtgaagaaaa aggaatcagg acttgcaagg gcaaaattaa gagggtcata
      541 cctgacactg gtgattagtg tttcattggt cctgttcctc cttgggatac ttggcctcgt
      601 ccttatcaat gcaaaggaat tgtcggatta tttcagggaa agcctgtcat tttcggttat
      661 gcttgatgat gaagccaggg aagcagacat aagaatgctt caaaaggatc tggatgcaaa
      721 accttatgta aaatcaaccg tctatgtatc aaaggatgag gctgcagcca agatgaaaga
      781 agacctggga gaggatttca taagttttct tggggataac ccgctgccac ccacaataga
      841 cgtttacctt tttgcagggt acacaagccc cgacagcgtt gcccggattg agaaatatgt
      901 gcttgaatat ccgtttgtca aggagatata ttatgaggag tcgctccttt tcctcattaa
      961 tgagaacgta agaaaggtga gcctctttct ccttgttatc agcgccttcc tttttcttat
     1021 tgcagtaacc ataataaaca acacaataag gcttgcaatt tattcaaaga ggtttctgat
     1081 aagaaccatg gaacttgtgg gagcaaccag gtcgttcata agaaaacctt tcctcgtcag
     1141 aagtgtttat catggaataa ttgcagccct gatagcgatg acattactta tgggtttact
     1201 gtatctcatt gagaaaaagt tttatatgat gttctatttc catagcatca ggcttcttgt
     1261 catcctctgt gttgccctgg taattcttgg catattgatc aatcttgttt caaccttttt
     1321 atcggttgac aggtatcttg atatttctga agataagtta tataattgaa tcatgtgaat
     1381 catggcactt aaaactgacg ataaggaaaa acaaaatttt gctcttggca gggagaatta
     1441 caagctcctg gcaatcggat ttgtcattat cgtggcgggc ttcctgctga tgctcggggg
     1501 cagatcggaa gatcccaatg ttttcgatga aaggatattc agcttcagga ggataacact
     1561 tgcacccatc atagttttgg cgggatttgt tttcgaaatc tgggccatta tgaaaaaacc
     1621 caaagatccc gcaaagtaaa atcaccacat tcttctaaac atgaatcttc tggaagccat
     1681 catcctggga ctgatacagg gactgaccga gtttttgcct gtaagcagtt cgggacatct
     1741 tgaactcgga aaatatcttt tcggtatcga tcatgaatca aatttctatt tctcggtcgc
     1801 agtgcacggg gcaactgttc tgagcacggt ttttgttttg tggaaagagt tgctcggcct
     1861 gttcaggggt gtaatgaaat tcacaatgaa cgaggaaacg aaatatgccc tgaaacttct
     1921 gatctcaatg atacctgtag gaatagtcgg actgttcatg agagacactg tcaaggaata
     1981 ttttacggga aacatgacga tgcttggaag ctttttcctc attacagctt tgttgctttt
     2041 catgacacgg gtaataaaac caaaggaaag ggatttaaca tatattgatt cctttattat
     2101 tggtattgca caggcttttg ccgtccttcc tgcaatatcc aggtcaggag ccacgattgc
     2161 aacgggaatg atgctgggga acaggaaatc ggaaattgcc aggttttcgt tcctgatggt
     2221 ccttgtaccg gttataggag ccaatattct tgaaattggt tcaggcgacc tgctgaatga
     2281 aggtacttca ttcgctgtta taagcgccgg gttcatcacc gcattcatat caggctatat
     2341 tgcctgcaga tggatgatta accttgtgcg caggggtaac ctggcatggt tcggcctgta
     2401 ttgtgtggct gcaggcattt tttcctttat actcgggatg aatggattat gaaaaggtat
     2461 ctgctgttga ggaaggaatg gtttttttgt ttgacaagcc cctttactgg acagcatttg
     2521 atctggtaaa caaggtcagg ataatgatca gaagtatcct tggcctgaaa aatgtcaagg
     2581 cgggacatgc cggaacactt gatccgcttg catcgggact gatgattatt tgcaccggaa
     2641 aggccacaag gaaaattgaa caataccgtg atctcgacaa ggagtatatt gccgaactgt
     2701 gcctcggggc atccacacct tcctttgatc ttgagactga gataattgaa agattcaggg
     2761 ttgatcatat tactgaagca tttgtccgtg ataagctgga ggagttcatc ggcgaacagg
     2821 accagatccc tccgatgcat tcagcaaaaa tgatcgaagg aaaaagagcc tatgagtatg
     2881 cacgaaaggg cctgaaaaag gaacttgccc cggtgaaagt tatattcagg gaactggaac
     2941 tgctctcgtt cagcattccg attgcaaaaa tcaggctggt ctgcagcaag ggaacatatg
     3001 tccgggcttt tgcaagagat cttggcatgg cgcttgaaag cggagcatac ctgtcatcac
     3061 ttgtcagaac ttcgataggt gaattcaaac ttgaaagtgc atattcactg gaaagtttta
     3121 aaaattttct ggaagatttg aaacaaacat gaaaattctt cgtataaagg aattcctaaa
     3181 ttcaaaatat aaattgtctt attatgaaat tgtcacaatt caggtacact ctgccgccgg
     3241 aactgattgc actttatccc gccgggaacc gcgatgaatc aaggttgctg gttgtaaaca
     3301 ggaaaacggg agcgctggaa cacagggtgt ttaaggaaat tacggagtat tttaatgagc
     3361 atgacgttct ggttttcaac aatacgaaag tttttccggc acggctgtac ggcaacaagg
     3421 agaaaacagg agccgagata gaagtgtttc ttttaaggga gctcaacagg gatcagcggc
     3481 tgtgggatgt gctggtcgat cctgcccgaa aaatcaggat agggaataag ctgtactttg
     3541 gtgaagacga tctcctggtt gctgaagtta tagataatac aacttccagg ggaaggacac
     3601 tccgcttcct gttcgacgga acctatgagg aattcaaaca gactctttac agcctgggcg
     3661 aaactccgct tcccaaattt attaaccggc cggtggaacc tgaagacagt gaacgttatc
     3721 agacgatctt tgcaaaacat gaaggcgccg ttgccgcccc aacggccggc ctgcatttca
     3781 gcagggaact gctgaagaag ctggagatca taggcgtgga attcgcggag ataaccctgc
     3841 atgttggact tggaaatttc cggagcgtgg atgttgaaga ccttaccaaa cacaaggttg
     3901 attcagaaag gatccggatt accgaggagg cagctgatat tgtcaataag gctaaagaca
     3961 acaaatacaa aatatgtgca gtggggacca cagttgtcag aactcttgaa agctccgttt
     4021 caacaaacgg atacctcaaa ccattcgacg gatggaccaa caagttcatc tttcctccct
     4081 atgaattcaa agttcccgat atgttcataa gcaacttcca cctgccatac tccaccctcc
     4141 tgatgatggt tgcagctttc gcaggttatg atctgctttt tgaggcatat aaaacagctg
     4201 ttaaggaaaa atacaggttt ggcacctatg gtgacgccat gctggtgata taggacggga
     4261 actgccaatc cactcccggg tatcattcgg tatcattatg ataatggccg gagcttaaaa
     4321 gcatccggct gttctgttaa tatattaaca ctaaaaatca ctcaggattt ttctttctgt
     4381 ctcccggctc attactttct atatttcagg atttagcccc ttccggaata atttctaaaa
     4441 ttcaatacag cctgttaaca tcctgttgat aacaaattcc ggaaaagtcc gtttataaag
     4501 ttgctgtaac ggcgtaatct gatcatttga tggtaaaatg ataccacctg gatgacagat
     4561 cggggcagcg ggtttttgaa ttttaacagt ttgaagctga atcctgaaat aacttctttc
     4621 agacggatat cttcattttc cgtaagatat tttcattgcc ggaaatctct ctggctgttc
     4681 agtgttttat ttttcatatt gagttcagtt aacgatgcct actcccagat cccatggcag
     4741 gccggttatg cctattgtaa aaagatcacc atacaggcga gccagataga aatatcaggg
     4801 tttggtccac ataataattt tcccgttctc atagatatta cggataatga tctgagaacc
     4861 tgttcatatg gcggatatat ggaaaatgcc tcaggctatg atatcaggtt cacaacttac
     4921 ctggggattc ggctgtatca tgatattgaa ggttacgacc caataagcgg cagactgacg
     4981 gcctgggtcc ggatcccttc gctttccaaa ataaataccg atatttttct cttttttggg
     5041 aatccctcaa tatcatcgaa ctcatcatcg tccgccacct ggagcagcaa ctataaagca
     5101 gtatggcatt tcaataactc gattgccaat gcatctgcct ttcccctggt atcctcggac
     5161 ttcggcagca ccagtaccac aggaaaaatt cttgaaggac ggaatttcat aagaacgtcg
     5221 caccaatata tcagtgtaaa ttataatgat gctcttgatt tcaatgacgc gattacagta
     5281 tcagcctgga taaatctggt aacaaacgat attgaccaga aaatattcag taatcaaaat
     5341 aacatcagtg gcggctataa gcttggaata tatactgacg ggaaggttga atttgaaatt
     5401 agaacatcag ataatcatcc ttaccttaac aggagtgcac cggatggtac tatactgtca
     5461 acttccacct ggtattatgt tgtgggagta tacagtaatt ctgaagatta cattagaaca
     5521 tatgtaaacg gcggccttga taggccaatg gttaccgata agactgcagg attaaccgga
     5581 agcggaaatc cggccatcgg cagggaaacc tggaatgacc caagtacctt tgacggaaaa
     5641 ttcaatggta taatcgatga actcagggtg caaaatattg ccagggacgc cggttggata
     5701 aaaactgaat tcaataacca gtcatcgcct tcaacattct acacagtcgg ccctaccgca
     5761 tcaaatcccg ttgcagtttg taaacccgtt accctatatc ttgatgcctc aggcaatgca
     5821 agccttaccg tacctatggt cgacaacgga agcagttcat attgcggatt ggtaaaaaca
     5881 ctgaacaaat actccttcag ttgctccaat ctgggagaaa acattgttac actgactgtg
     5941 gcagatcctt ttaatactgt tacatgcaat tctacagttt tggttcggga tgccatcccc
     6001 ccggtaatat cgaactgtcc tgcggacatt tctgtaagtg ccgatgcggg aagctgcgga
     6061 gccagggtga actggaccga gcccacggta tcggataact gtaccccgac agttaacatt
     6121 gtctggatca aatcaaaact acccggaaat ttttttccta tcgggaccac gcaggtcaac
     6181 tatatcgcac gggatgcttc agggaatacc agtacttgct catttaccgt aacggtgaat
     6241 gacaacacca tgcccctgat taactgcaaa gtttccggcc agcagaatgt tccggcaaat
     6301 actaactgta aatatgttca tagcggcact ggctgggatg ccactgctag cgacaactgc
     6361 actgtcagtt ccctggcata cacgctcacg ggtgccacca gcgggagcgg aagcagcctg
     6421 gataatgttg tattcaacat tggtcttaca acagttaact gggaagcaac agatgccgcc
     6481 ggaaataaat cgcaatgcag tttcacggtc aatgtaaccg acaatatcat tcctgtagta
     6541 atatgccccc ctgacataac acatgacagt gacccgggaa tgtgcagcta cacttttaca
     6601 ccggcacagg ccacaggaac cgataactgc ggaatagatt acgtgaccgg gacgagaagc
     6661 gacggacttt tgctttcaga tccatatcct gtgggagtga caacaatcac ctggacttcc
     6721 actgatctga acggaaatac cagccttccg tgttatcaga agattactgt tttggataaa
     6781 gaaaagccag tgtttgcggc accaccggca gttactgtct gccgttcttc agattgcagc
     6841 ttcgactcaa atattattcc ggcaattacc ggtaatgttt tcggtgaatc agataattgc
     6901 acaccccagg ctttgttgga gatagaacat agcgatgatc tttctggcat aactgattgc
     6961 aatacagccg gcacaatact gaggacgtgg aaagtcacag atgaagcagg gaattttacc
     7021 gaaaaggtcc aggcaattgt tgtagaaccg cttccaactg tcacagtagg gaatgacgta
     7081 tcatcgatct gcaccggcgg gaatgccgat atagttatta acagttcaac cgtaaattca
     7141 ataccttcag atttcagtat tgatgtaaag gtgacatcga ccgatcccgg taatctgagt
     7201 ggtaccgccc ccctggcaga ctttgttctt gcaaaatccc agctgcctta taccattaac
     7261 gggacattga ttaacaattc aggcaatccc attgaagtta cgttcaggtt cacgccgaaa
     7321 attgcaggtt gtaatgatgg ctcgacagtg agtactgaag tcacggtcca ccccgatccg
     7381 gcatgggggg ccatagtagt aaccccggga aatatctgcc ttgaagggga agtcactttt
     7441 tcagccgagc ttaacggttc cgccgggggc agcgtagagt ggatacggtc ggagacatca
     7501 ggtggggcag gcattgtggt ccacagccca gacatacctt tatcaccggg aacatattat
     7561 taccgtcctc actatactgg cggctacggt ggctgcaacc tttcagacgg cacggagact
     7621 atggtcacgg tccaccccga cccgtcgtgg ggcacgataa atacacccca gccggatatc
     7681 tgcgttggcg
//
LOCUS       DAOOYG010000130         7635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10819_length_7635_cov_6.178628, whole genome shotgun sequence.
ACCESSION   DAOOYG010000130 DAOOYG010000000
VERSION     DAOOYG010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10819_length_7635_cov_6.178628"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PK288_09750"
     CDS             <1..1197
                     /locus_tag="PK288_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPM19249.1"
                     /translation="ECHWARACDQVISDSICMSSHKAALQGSDHKRVPNDIHINPTII
                     GHEFAGVILQVGKKWKHRFSPGQKFSIQPAMAHTGTLDAPGYSYRYIGGDATHIIIPG
                     LVMEADCLLEYDGEAFFPASLSEPMSCIVGAFNASYHVPPGTYRHEMGIKPGGKMAIL
                     AGVGPMGLGAIDLALNHKRKPSILVVTDIDDSRLARAACLFPPEKAAGKGVKLIYVNT
                     AKITEPEKTLRDLTGGTGYDDVFVFAPVKQVVEQADAILGFDGCLNFFAGPTNTEFKA
                     EINFYNIHYSFTHIMGTSGGNTDDMKESLRLMAEGSINPAVMITHVGGLDSVIDTTLN
                     LPQIPGGKKLIYTNISLPLVSLYDLGEKGRNDPFYAGLHEIVSKNNYIWSLEAEQYLL
                     ENAKPI"
     gene            complement(1296..2231)
                     /locus_tag="PK288_09755"
     CDS             complement(1296..2231)
                     /locus_tag="PK288_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPM19250.1"
                     /translation="MRKIYGIGETVLDIIFKNGQPLAAKPGGAMLNSIVSLGRIGLPV
                     HFISEYAGDQVGTLIDRFLHENGVDTSFVYRFRDGKTKLALAFLDDRNDAEYTFYEKY
                     SEKRLETEFPAIGRDDIVLCGSIYAVTAEIRTRFRDFINRSRDNGALIVYDPNFRSAH
                     RHELARLKPLIIENMQTGTLIRGSDEDFRNIFDANTPDEAWEAVGNYCGCLVYTASAR
                     GVYVRTRSFSGQFPVKRITPVSTIGAGDNFNAGMIAAIWLKGISADEPECMDLVRWTE
                     VISMGVDFATEVCMSYENYIPEQFARMLKEKMKIT"
     gene            complement(2242..2784)
                     /locus_tag="PK288_09760"
     CDS             complement(2242..2784)
                     /locus_tag="PK288_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPM19251.1"
                     /translation="MRILKENAAAVVIDIQEKLFPHMHESEKLLRNCQKLIDGLQTLE
                     VPLIVTQQYTKGLGPTIPAISFRFFNFSYFEKMTFSCFDDPIIREQMNSLNKPVLILF
                     GIEAHVCVMQTCIDLIDNGVTTVVVEDCISSRNPDNKKTAIERMRQEGAVITGYESLL
                     FELTRTSANDVFRRISGIVK"
     gene            3335..4663
                     /locus_tag="PK288_09765"
     CDS             3335..4663
                     /locus_tag="PK288_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HPM19252.1"
                     /translation="MRTLSRRKFIVSGAIAGLGTTAVLPACRNGQDGTASGKTRNHPL
                     DGIEREKLVITDVRVTLLSCEIPPDEQWYLDWIPERYKAWKTDSILVEVFTDKGFRGI
                     GGATQYGGPEVVKQYIESIIKPAVIGKNPFDIEYLTCGVKKRGPMVGWAGVDAALWDI
                     IGKAKGKPVYEFLATDHPPLRKIPVYASAGEMYDGDVWPDNLIEEALKLREKGFKAYK
                     FRPGTHWSVSGMTMSRYIDGVWKIRDAMGPEFGLIQECNAQWNMEQTMQFIPEAEKMK
                     LIWIEDPVERLGPEAKENYSRIKDALKTVKLSYGGDVMDNRFDYKEWIDRNAMDIVQP
                     DAGVMGLTEAWYVSRMAHLQNQLCAPHNWHGALLTIANASLAAGIPNLMMLETNQTYN
                     PLRTDLFKEPLVPVDGFIELPEGPGFGVEVIDDVEKRFPFIPGRYDKPRE"
     gene            4706..5743
                     /locus_tag="PK288_09770"
     CDS             4706..5743
                     /locus_tag="PK288_09770"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPM19253.1"
                     /translation="MKAMQLTGIRRIEMTDLPEPVISGPKDVKIKLSVLGICGSDIHY
                     YVNGRIGSQVVSYPFAVGHECAGIVIETGKNVRRVKPGDVIAIEPAMPCWECDQCLAG
                     RHHTCRRLKFLGCPGQSEGSLMEYIVMPEESCFPLTGNLNADHGAISEPLAIGVYSVR
                     KSGDIKNLSAGILGFGPIGMSVMLAAKAAGAGQIFVTDKIDRRLDIAEMEGADAVMNP
                     LREDIVAGIGGISRSGLDIVFECCGEQEAVDQAVEILKPGGKLIIVGIPEFEKWKFDA
                     DRTRRKEISIQFIRRQVDCVQPSLDMMASGEINIDRMITHRFPFTETKAAFDLVAGYH
                     DGVMKTMIDFE"
     gene            5803..>7635
                     /locus_tag="PK288_09775"
     CDS             5803..>7635
                     /locus_tag="PK288_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPM19254.1"
                     /translation="MLTCTAHGLNEPQIRERNILASEAKAIDLDRSLITDNSWIRLPG
                     YKNRQFWQHLPSNLKEEYIKKAESFLSYNWPVVKATDYLEYIRSGDRRQEVYAACSNA
                     LISLVMGELVEGRGRFTDQIINAVWYYCEQTWWGWSAHLGPQKAGAGLPDINDPVVDL
                     GVGEVASNLSWTWYLFKDEFNKIHPLIASRLKQEIRSKVLEPYFERNDFWYMGFEGGR
                     PNNWNPWVNYNMLNCFLLIEDDPVRKRAQVMKIINSLDKFLNGYPDDGGCDEGPSYWG
                     AAGALLYECLEVLKDATGGKFDVFDNPLVQNMGKYFYKVNIHAPYFINFADADATTSG
                     SPPVVYRYGKAIRDPLMQQFGAYLASLAKWGEHTPTGKICDQVKSLLLLDEISDAEKK
                     EALVADFWFPDTEIAGARDREGSFMGFFFGAKGGFNAESHNHNDVGSCLMYYDGKPCL
                     IDLGREEYVAKTFGPQRYEIWTMQSGYHNLPVINGTDQKDGAEFRAVNSRFSANPKTA
                     TFSTDIAGAYPSEARVRSWVRSYTLSRGKSFVIKDRYELTEQTGRPTSANFITYCRAT
                     VVKPGLIRLDGDGFSLNMAFSSKSVSPEIEFMEIKDRTLKRYWPV"
BASE COUNT         2108 a         1808 c         1866 g         1853 t
ORIGIN      
        1 gaatgtcatt gggcacgcgc ttgtgatcag gtcatatcgg acagcatctg catgtcgtca
       61 cacaaggcag cgctgcaggg ctctgatcac aagcgcgtgc ccaatgacat tcatattaat
      121 cccaccataa ttggtcatga atttgccggc gtcatcctgc aggtcgggaa aaaatggaag
      181 caccggttca gtcccggtca gaaattctcg atccagcctg caatggcaca cacgggaact
      241 ttggacgcac cgggctattc ctacagatat atcgggggtg atgccactca tatcatcata
      301 cccggcctgg taatggaagc cgactgcctc ctggagtacg acggtgaggc atttttccct
      361 gcatcgcttt ccgaaccgat gtcgtgcata gttggagcat tcaatgcctc atatcatgtt
      421 ccccccggaa catacaggca tgaaatggga ataaaaccgg ggggcaaaat ggcaattctt
      481 gccggagtgg gtccgatggg tctcggagcg atagatcttg ccctgaacca taaaagaaag
      541 ccgtccatcc tggtggtgac cgacattgac gactcaagac tggcaagggc agcctgcctt
      601 tttcctcctg aaaaggcagc cggcaaaggc gtgaagctga tctatgtcaa taccgcaaag
      661 attacagaac cggaaaaaac cctgcgagac cttacaggag gtaccggtta tgatgatgtg
      721 ttcgtttttg ctcctgtaaa acaggttgtt gaacaggcag atgcgatcct tggcttcgac
      781 ggatgcctta atttttttgc cggtcccaca aacaccgaat ttaaggctga gatcaatttt
      841 tacaatatcc actattcatt cacccatata atgggaacgt cgggcggaaa caccgatgat
      901 atgaaggaat cgcttcgact aatggccgaa ggcagcataa atccggcagt aatgatcact
      961 cacgttggag gtcttgactc agtgatcgac acaaccctga acctgccgca gatacccggt
     1021 ggtaaaaaac tcatttatac caacatatcg ctaccattgg tttcccttta cgacctcggg
     1081 gaaaaaggca ggaacgatcc tttttatgcc ggactgcatg aaatcgtatc aaaaaacaat
     1141 tatatctgga gcctggaggc tgaacaatat cttctggaaa atgcaaaacc catatagcct
     1201 aatggaaacc aggcagatat ttgtagttat cggagaattc atccgcagcc tgacgtatcg
     1261 ctgaacagcg ggcttacctc ccgtataaaa gcagatcagg taattttcat tttctctttc
     1321 agcatccgcg caaactgctc cgggatatag ttctcataac tcatgcaaac ctcagtggca
     1381 aaatcaactc ccatggaaat aacctcagtc catcgaacaa gatccataca ttcaggctcg
     1441 tccgctgaaa tgcccttcag ccagatcgcg gcaatcattc cggcattgaa attatctcct
     1501 gctcctattg tacttaccgg ggtgatcctt ttaacgggga attgtccgct gaaacttctc
     1561 gtccttacat atacacctcg tgcacttgcc gtataaacaa ggcatccgca gtaattcccc
     1621 acggcctccc aggcctcatc gggggtattg gcgtcaaaga tgttcctgaa atcctcatcc
     1681 gaacccctga taagagtccc tgtctgcatg ttttcaatga tcagcggctt cagcctggca
     1741 agctcatgcc ggtgggcact cctgaaattc ggatcataaa ctatcagtgc cccgttatcc
     1801 cgggacctgt tgataaaatc cctgaatctg gtccgtattt ctgcggtgac ggcataaatt
     1861 gatccgcaaa gtacaatatc gtccctgccg attgcgggaa attccgtttc cagcctcttt
     1921 tccgaatatt tctcataaaa cgtatattcc gcatcgttcc tgtcatcaag aaaggccagg
     1981 gcaagttttg ttttcccgtc cctgaaacgg tacacgaacg aggtatccac gccgttctca
     2041 tgcaggaaac ggtcaatcag agtgccaacc tgatccccgg catactcgct tatgaaatga
     2101 accgggagac cgatcctgcc aagtgaaaca atgctgttca gcatcgctcc tcccggcttt
     2161 gctgccagcg gctgaccgtt cttgaagata atgtcaagaa ccgtctcccc tatcccgtat
     2221 atcttcctca tggctgacag gtcattttac tatccccgaa atcctcctga acacatcatt
     2281 tgcggaagta cgtgtaagtt caaacagcag gctctcataa ccggtaatca ccgccccctc
     2341 ctgtctcata cgttctatcg ctgttttttt gttgtcgggg ttccttgatg atatgcaatc
     2401 ctccaccaca accgtcgtga caccgttatc aatcaggtca atgcatgtct gcatgacaca
     2461 aacatgcgct tcaatgccaa aaagaatcag aaccggcttg tttaatgaat tcatttgttc
     2521 ccttattatc gggtcatcaa agcagctgaa agtcattttt tcaaaataac tgaaattgaa
     2581 aaacctgaat gaaattgccg ggatagtcgg accaagccct tttgtgtatt gctgggtcac
     2641 aattaacggt acctcaagtg tctgaagccc gtctataagt ttttgacaat ttctcagcag
     2701 cttttcactc tcatgcatgt gaggaaaaag cttttcctgg atgtcaataa caacagctgc
     2761 cgcgttttcc tttaaaattc tcatctttta taactgaaga tgtgaatata caaaatttta
     2821 acattagcag aacaaaccga ttttgaagta ttcataaatc ctggctcatt gataaatata
     2881 tttcgtatcc ccatatcgcc cggttatttc ccctcccata cacctctccc taaatccctc
     2941 cccccagggg gaaggacttg aagaacccca taattattaa ccatctcaaa caaacagaga
     3001 tttataactt tgcagacaac tatcgttccc cgctctcccg ggagcggggg ataagagggg
     3061 gtgaggtgaa tcaatacacc tctccctaaa tccctccccc tggggggagg gactttaaga
     3121 acaccataat tatagaccat atcaaacaaa cagagactta taaccttgca aacaactatc
     3181 atcccccgct ctcccgggag cgggggataa gaggggtagg gaatacctgc agcatggaaa
     3241 aagagatgat ggaaatgtca aagcatgtta tcagggagaa atttgaggtc attttaataa
     3301 tttaccttac tttagactgt taatattttt ctccatgaga actctgtcca gacgtaaatt
     3361 catagtatca ggagccattg caggtcttgg tacaacagct gttttgccgg cctgcagaaa
     3421 tggccaggat ggaacagcct ccggtaaaac cagaaatcat cccctcgatg gtatagaacg
     3481 ggaaaaacta gtcattacag atgtcagggt cacactcctg tcatgtgaga tccctcccga
     3541 tgaacaatgg tatctcgact ggatcccgga aagatacaag gcatggaaga ccgacagtat
     3601 tcttgttgaa gtcttcaccg acaaggggtt caggggaata ggaggagcaa cccagtatgg
     3661 cggacccgag gttgtaaagc aatatatcga aagcatcatc aaaccggctg tcatcggcaa
     3721 gaatcctttt gatatagaat atctcacatg cggggttaaa aaaagggggc ccatggttgg
     3781 ctgggcgggt gtggatgccg ctctgtggga cattattgga aaggccaagg gcaaacccgt
     3841 ttatgaattc cttgcaactg accatccgcc tctgaggaaa atacccgttt atgccagtgc
     3901 gggcgaaatg tacgatggag atgtgtggcc ggataatctt attgaagaag cattaaaact
     3961 cagggaaaaa gggtttaaag cttacaagtt ccgccccggt actcactggt cggtgagtgg
     4021 aatgacaatg agcagatata tcgatggtgt atggaagatc agggatgcaa tgggcccgga
     4081 gttcgggctt atccaggaat gcaatgccca gtggaacatg gaacagacca tgcaatttat
     4141 ccccgaagct gaaaaaatga agctgatatg gatcgaggat cctgtggaaa ggcttggacc
     4201 ggaggcaaag gaaaactaca gcaggataaa agatgccctg aaaactgtaa agctttcgta
     4261 tgggggggat gttatggaca acaggtttga ctacaaggaa tggatcgatc gcaacgcgat
     4321 ggacattgtt caaccagatg caggagtgat gggtcttacc gaagcatggt atgtttcgcg
     4381 gatggctcac ctccagaatc agctgtgcgc tccccacaac tggcacgggg ccctgttaac
     4441 cattgccaat gcctcgctcg ctgccgggat accaaacctg atgatgcttg aaacgaatca
     4501 gacttacaac cctctccgga ccgacctttt caaggaaccg cttgtcccgg ttgacggatt
     4561 cattgagctt cctgagggtc ccgggttcgg ggttgaggtg atcgatgacg ttgaaaaaag
     4621 attccctttc ataccgggaa gatatgacaa gcccagggaa taaggagtga attcaggact
     4681 aaatcattaa taaatctggc agtcgatgaa ggcaatgcag cttacaggaa taagacggat
     4741 agaaatgacg gatctgccgg aaccggtcat ttccgggcca aaggatgtaa agataaagct
     4801 cagcgttctt ggtatctgcg gatccgacat acactactat gtcaacggca ggattggctc
     4861 tcaggtcgta agctatccct ttgccgttgg tcatgaatgt gccggtattg ttatcgaaac
     4921 cgggaaaaac gtaagacggg tgaaaccggg cgatgtaatt gccattgaac ctgcaatgcc
     4981 ctgctgggaa tgcgaccaat gccttgccgg caggcatcat acatgcaggc ggcttaaatt
     5041 ccttggctgc ccgggccagt ccgaaggcag cctgatggaa tacattgtca tgccagaaga
     5101 atcgtgcttt cccttaaccg gcaacctcaa cgccgatcat ggagccattt ccgaacctct
     5161 tgcaatcggt gtctattcag tcaggaaatc aggagatatc aaaaaccttt ctgccggcat
     5221 cctgggattc ggacccatag gaatgagcgt gatgcttgca gcaaaagctg ccggggccgg
     5281 tcagatcttc gtcactgaca agatcgacag gagacttgat attgctgaaa tggaaggtgc
     5341 ggatgctgtg atgaatcccc tcagggaaga catcgttgca gggataggcg gaatttcacg
     5401 gtcgggactg gatattgttt ttgagtgttg cggagaacag gaagctgtgg atcaggccgt
     5461 ggaaattctt aaacccggcg gaaaactgat cattgtaggt attccggaat ttgaaaaatg
     5521 gaaatttgat gccgaccgga caagaagaaa ggaaataagc attcagttca tcaggcgcca
     5581 ggtcgattgc gtgcagccat cacttgacat gatggcatca ggtgagatca atatcgacag
     5641 gatgatcacc cacaggtttc ctttcacgga aacaaaggct gccttcgacc ttgttgcagg
     5701 ataccatgac ggggtaatga aaacaatgat tgattttgag taaacgatat ggaatcaaaa
     5761 aacttcaacg gtaaattgtc agctgcaggg tttctattgt tgatgctgac ctgcacagcc
     5821 cacgggctga atgaaccgca gatcagggaa aggaacattc ttgcctccga agcaaaagcc
     5881 attgatcttg atcgttcact tatcacggat aattcatgga tcaggcttcc cggttacaaa
     5941 aacaggcaat tctggcagca tcttccttca aacctgaagg aggaatatat taaaaaggct
     6001 gaaagtttcc ttagctataa ctggccggtg gtaaaagcca ccgattatct tgaatacatc
     6061 cgctcgggcg acagaaggca ggaagtatat gcggcatgca gcaacgcact cattagcctg
     6121 gtgatgggtg agctggtcga aggacgggga cggtttactg accagatcat caatgctgtc
     6181 tggtattact gcgagcagac ctggtgggga tggtccgcac accttggtcc gcagaaggcg
     6241 ggagccggcc tgcccgacat caacgatccg gttgtggatc ttggtgtcgg agaggtagcc
     6301 agcaatcttt cgtggacatg gtatttattc aaggatgaat tcaataaaat acatcctctc
     6361 attgcttcaa gactgaagca ggaaataaga agcaaggtcc tggaaccata ttttgaaagg
     6421 aatgatttct ggtacatggg atttgaggga gggcgcccca acaactggaa cccatgggtt
     6481 aactacaaca tgcttaactg cttccttctg attgaggatg atcccgtaag aaagagggcg
     6541 caggtgatga agatcataaa ctcgcttgat aaattcctga acggatatcc cgacgacggc
     6601 ggctgcgatg aaggtccgtc atactggggc gccgcagggg ctttgctgta tgagtgcctg
     6661 gaggtactta aggatgccac cggcggtaaa tttgatgtat ttgacaatcc gcttgttcag
     6721 aatatgggta aatattttta taaagtaaac attcatgcgc catactttat aaattttgcg
     6781 gatgccgatg cgactacaag cggcagtccc cctgtcgttt acaggtatgg gaaagccata
     6841 agggatcccc tgatgcagca gtttggagca taccttgctt cacttgcaaa atggggagaa
     6901 catacgccca ccggcaaaat ctgtgatcag gtaaaaagcc ttctcctgct ggatgaaata
     6961 tcggatgcag aaaaaaagga agcactggtt gccgacttct ggttccccga tactgagatt
     7021 gcgggagccc gcgacaggga aggcagcttc atggggtttt tctttggggc aaaaggcggt
     7081 ttcaatgccg aaagccataa tcacaacgac gtgggttcat gcctgatgta ttacgacggc
     7141 aagccctgcc tgatcgacct tgggcgtgaa gaatacgttg ccaaaacctt cggtccgcag
     7201 cgctacgaga tatggacaat gcagtcgggc tatcacaatc ttcctgtcat caacggcacg
     7261 gaccagaaag acggggctga gttcagagct gttaattcaa ggttttccgc aaacccgaaa
     7321 accgctacgt tttcgactga tattgccggg gcttatcctt ccgaagccag ggtcagaagc
     7381 tgggtccgta gctataccct cagccgcgga aagagcttcg tgataaaaga caggtatgag
     7441 cttactgaac agaccggcag gccaacaagt gccaatttta tcacttattg cagggcaaca
     7501 gtggtaaaac ccgggcttat ccggcttgat ggcgacgggt tcagccttaa catggctttc
     7561 agttcaaaat cggtgtctcc tgaaatcgaa tttatggaaa taaaagacag gacacttaaa
     7621 cgttactggc ccgtt
//
LOCUS       DAOOYG010000131         7600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10888_length_7600_cov_9.140888, whole genome shotgun sequence.
ACCESSION   DAOOYG010000131 DAOOYG010000000
VERSION     DAOOYG010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10888_length_7600_cov_9.140888"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PK288_09780"
     CDS             <1..392
                     /locus_tag="PK288_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPM19255.1"
                     /translation="GFFVGPTILENVDVNSEIYNTEIFGPVLIIKNVDSLDEAIKLAN
                     HGNHGNAASIFTTSGAAARKFRHDVQAGNIGINIGIAAPMAFFPFSGWKESFFGDLHG
                     QSFHAVEFYTQTKVVIERWHDEWTRKF"
     gene            428..1327
                     /locus_tag="PK288_09785"
     CDS             428..1327
                     /locus_tag="PK288_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPM19256.1"
                     /translation="MIRIANAPCSWGVLEFDLEGKAAGYEQVLDEIRETGYQGTELGD
                     WGFMPTMPSELSKELDKRSLALVGAFVPVFLKDRTRHAPGAETAVKTARLMADAGYPD
                     SFIVLADENGSIKERTDNAGRVTPSMGLSKDEWKVFAEGANFVAGEIKNKTGLRTVFH
                     HHCAGYAETPEEIDSLMALTDPSLVGLVLDMGHYMFGGGDPIIALRRHKDRIWHVHFK
                     DCHPEIAAKSRKEGWDYFKSVANGVFCELGKGAVNFKEIVGELHAQKYSGWIVVEQDV
                     LPGMGNPKVCAQRNRDFIRKLGL"
     gene            1350..2366
                     /gene="iolG"
                     /locus_tag="PK288_09790"
     CDS             1350..2366
                     /gene="iolG"
                     /locus_tag="PK288_09790"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HPM19257.1"
                     /translation="MKKLKLGVIGAGRIGKVHIATLVQSVPQAEVVALAETNPAVASE
                     VAKMFGITSVFTTYMDVIRHPDVEAVVICSPTDTHAKYIVDAANAGKHIFCEKPVDLS
                     LDVIKGAIKAVEKAGVKLMVGFNRRFDPNFSKIRQLVATGKIGDPHILKITSRDPAPP
                     PAEYSAVSGGMFMDMTIHDFDMARFITGSEVTEVYAKSAVLVDPEIGKAGDIDTAVIT
                     LSFANGAIGVIDNSRKAVYGYDQRVEIFGSKGMACADNDFPDNHRYYTDDGIHGSLPL
                     NFFMDRYLDAYANEMKVFCDAVLNDKQPPVTGTDGLRSVLIAMAAKKSYLENRPVKLS
                     EIES"
     gene            complement(2394..3149)
                     /locus_tag="PK288_09795"
     CDS             complement(2394..3149)
                     /locus_tag="PK288_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPM19258.1"
                     /translation="MTDPQFGFLDNKNFSAETVLYEKAVEKINNLRPAFVVVTGDFVN
                     DKSNRAQWDEFDRITGEIDKTIPVWLSPGNHDIGMPPGKDDLDKYISKYGYDRFSFRI
                     RKSLFIGLNTCLIKSGNNEQEQIQYDWLQQKLKTGKKARHIILFGHYPFVINSPDEAE
                     TYSNIAPDLRKEYLNLFKEYGVTTVFSGHLHNNAASSSGETEMVVTSAVGRPLGDAPS
                     GIRIIKVYKDRIEHIYYPLDQVPDKVIFNRKGR"
     gene            complement(3287..4372)
                     /locus_tag="PK288_09800"
     CDS             complement(3287..4372)
                     /locus_tag="PK288_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPM19259.1"
                     /translation="MRKTIILFLLLAVTDLLSSGQKSSLIVLGDLHYDNIRLHDMNWL
                     MDKPDDLRQVKEYTEITENNWDDFMSILRNKVLNVRPQVKALIQLGDLSEGLAGTEEK
                     ADQMAREIVRAVETAETGVPWIITKGNHDITGPGAVDAFLSHYTPMLRKQTGNSGIMN
                     ASYTYRHGNIQITCIDPWDKSTDMVKFLDEELSSSDAVVKFVAIHEPVIPVTERCWHT
                     LRRDPVRREKLLEVIARNKAIVLCAHLHRYSVVRRNTDYGPVVQVMAISVVSDRDYFK
                     PDKLITEYGPSLAENVPDWEPSTIESRKAILAEEAEFVTFYKQTDLPGYALIKIDEKK
                     GTVKLEYYAAFSKKAYDKVNLSGLLRK"
     gene            4531..5649
                     /locus_tag="PK288_09805"
     CDS             4531..5649
                     /locus_tag="PK288_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM19260.1"
                     /translation="MKNSRILAIITGFIFSCSLISFSQSGNVLKIFQFPADRIPRIDG
                     NSDDWDIVPAGYEIGSYLLRDDEKKHSAPDPANLDVKVKVGWVKGMNKLYFLYEAYDN
                     YWDFSKVGLHHDIFEIVVDADQSGGPLIEQFHPNPDLNNTWEAYFNFHGVHAQNYHIF
                     TPAEGEDWCLAWGSQPWIKELPYANAAYKYDFRPGESGKLVLEFWITPFDYAGPEGPV
                     RAVESVLEEGRDIGLCWAVIDFDDVNARSNNGFWNLSEHHTMYGNASQLRTFRLMPLE
                     QQFRKKIDAQWSFRIIDMDRRMVAFRDQSAGQINSWKWDFGDGTTSSDQHPVHTYTKP
                     GRYIVILYIEGPDGKSRMGKMWDVAVRSGRLDRDVKSN"
     gene            5945..7309
                     /locus_tag="PK288_09810"
     CDS             5945..7309
                     /locus_tag="PK288_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPM19261.1"
                     /translation="MRLKSIILVIMAGMSIPAVSQLKVTDTNTPLHAMQPDYPVAYGP
                     AKAEDIKAVLNRLFNYLDESTPAKLVDPQTKAEIMDLKKMTPGTVFGQGVFRLISYEW
                     GVTYGAMLLAADATGDPKFRDYTTKRMELISAVSRYYKELPPDRQKNNPVRSVLEPRA
                     LDDAGSMCAAMIKTLGSGNNPSLRPFIDNYIDYISSKQQRLADGTLSRNRPLPDALWL
                     DDLYMSVPALAQMGKMTGDRKYFDDACKQIIQFSQRMFNAEKGLFMHGWIQGMNPHPE
                     FYWGRCNGWALLAMSELLDVLPADHPSVKTILELFRSHAAGLASYQSDTGFWHQLIDR
                     NDSYEETSATAIFTYCFSHGINKGWLDARAFGPAAVLGWNAVQTKVNDLGQVEGTCVG
                     TGMAFDPAFYYHRPVNVAAAHGYGPVIMAGAEILTLMKNYQIVINDSAVMFYPAGTDW
                     KSYR"
BASE COUNT         2144 a         1596 c         1776 g         2084 t
ORIGIN      
        1 aaggattttt cgtcggccct accattctgg agaatgtcga tgtcaacagt gagatttaca
       61 atacagagat cttcggcccg gtacttataa tcaaaaatgt tgattcactt gacgaggcaa
      121 ttaagctagc aaatcatggg aaccatggta atgcagccag tatattcaca accagcgggg
      181 ccgctgcccg taaattcaga catgatgttc aggcagggaa tatcgggata aatataggca
      241 ttgcagctcc aatggcattc ttcccgttca gcggctggaa agagagcttc ttcggcgacc
      301 ttcacggcca atcgttccat gcagtggaat tttacaccca gacaaaggtc gtcatagaaa
      361 gatggcatga cgaatggaca aggaaatttt aatttaaaat gtgtgaaacc agtaaaacct
      421 ctataaaatg attagaattg caaatgcccc ttgttcgtgg ggcgtcctcg aattcgacct
      481 tgaaggcaag gctgccggct atgaacaggt ccttgatgag atccgcgaaa cgggatatca
      541 gggaactgaa ctgggagact ggggctttat gcccacgatg ccttcggaac tgagtaaaga
      601 gcttgacaaa cgctctctgg ctttggtggg cgcctttgtt ccggtattcc ttaaagacag
      661 aaccagacat gcaccgggag ctgaaactgc tgttaaaacc gcccggctga tggcagatgc
      721 aggctatcct gattccttta ttgttctggc agatgaaaac ggcagtatta aagaacgtac
      781 tgacaatgcc ggaagagtaa ccccctcaat gggtttatca aaagatgaat ggaaagtgtt
      841 tgccgaagga gcaaattttg tcgccggcga aattaaaaac aaaacaggtc tccggactgt
      901 atttcatcat cattgtgccg gatatgcaga aactcctgaa gaaatcgaca gtctgatggc
      961 attgactgat ccgtcacttg tcggcctggt cctggatatg ggccattata tgttcggagg
     1021 cggagatccc atcattgcct tgagaagaca taaagacaga atctggcatg tccatttcaa
     1081 ggattgtcat ccggaaatag cagccaaatc aagaaaagaa ggatgggatt atttcaaatc
     1141 cgttgccaat ggagtattct gtgaactcgg aaaaggagct gttaatttca aagagatcgt
     1201 tggggagctt cacgcacaaa aatattcggg atggatcgtt gtggaacagg atgtactgcc
     1261 cggaatggga aatcccaaag tatgtgctca acgaaacagg gattttatca ggaaactggg
     1321 attataatat taaagtcaat tttattaaaa tgaaaaaact gaaattggga gttataggtg
     1381 ccggaaggat cggcaaggta catatagcaa cgctcgtaca gagtgttccg caggctgaag
     1441 tggttgcact tgccgaaaca aatccggcag ttgccagtga agtggcaaag atgttcggga
     1501 ttacttctgt attcactacc tacatggatg tgataaggca tcctgatgtt gaagctgttg
     1561 taatctgttc tccgacagac actcatgcaa aatacatcgt tgatgcggct aatgcaggga
     1621 agcacatttt ctgcgaaaag cctgttgatc tgtccctcga tgtaattaaa ggggctatta
     1681 aagctgttga aaaagccggt gtaaagctta tggtcgggtt caaccgccgc ttcgatccca
     1741 acttctcaaa gatccgccag cttgtcgcta cagggaaaat aggtgatcct catatcctga
     1801 agataacttc ccgcgacccg gctccgcctc ctgcagaata cagcgctgtt tcaggaggta
     1861 tgttcatgga catgacaatt catgatttcg acatggccag gtttataaca ggcagcgagg
     1921 tgacagaagt ctatgcaaaa tccgccgtac ttgttgatcc cgaaataggt aaagccggag
     1981 acattgacac tgcagtcatc actctcagct tcgcaaacgg agcaatagga gtcattgata
     2041 acagccgtaa agccgtttat ggatatgatc agcgtgtgga aatattcggt tcgaaaggaa
     2101 tggcatgtgc tgataatgat tttcctgata atcacaggta ttatacagat gatggcatac
     2161 atggttcact tcctcttaac ttctttatgg acaggtatct cgatgcctat gcaaatgaga
     2221 tgaaggtatt ttgcgatgcg gtattaaatg ataaacagcc tccggttaca ggtacagatg
     2281 gattaaggtc ggtcctaatc gcaatggcgg caaaaaaatc gtatctggaa aacaggccgg
     2341 tgaagctgag tgagatagaa tcatagactt gcctttgcgt actttgcgga accttagcgc
     2401 cctttgcggt taaatataac tttatcgggt acttgatcca aaggataata tatatgctct
     2461 atccggtctt tatagacttt aattatcctg atccctgaag gcgcatctcc aagcggtctg
     2521 ccgacagccg atgttacaac catttctgtt tcgcctgagg aagatgctgc attgttatga
     2581 agatgacccg aaaaaaccgt cgtcactcca tattctttga acagattgag atattccttc
     2641 cgcagatccg gagctatatt tgaatatgtt tctgcttcgt caggactatt aataacgaaa
     2701 ggataatgcc cgaaaaggat tatatgtctg gcttttttac cagtttttaa tttctgctga
     2761 agccagtcat attgtatctg ttcctgttca ttattacctg atttaataag acaggtattc
     2821 aatccgatga ataggctttt cctgatcctg aaggagaacc tgtcgtaacc gtacttactt
     2881 atatatttat cgagatcatc tttccccgga ggcataccta tatcgtgatt tcccggagaa
     2941 agccagaccg gtattgtttt atcaatttcc ccggtgatcc tgtcaaattc atcccactga
     3001 gccctgttcg acttatcatt aacaaaatca ccggtaacga caacgaaagc cggtcttaaa
     3061 ttgtttattt tttctaccgc tttttcgtat aaaaccgttt ccgctgaaaa atttttatta
     3121 tccagaaacc caaactgagg atcagtcatc tgcacaataa aaaacggata agccggtttt
     3181 tgagcagaca gattacctga cgtaataaat aacgatatca ggcagattaa caggagtata
     3241 ttgtttctct tcatttgttt aacggattat ttcctcatat aacagattat tttctcagga
     3301 gcccggagag atttacttta tcgtaagcct ttttactgaa agctgcataa tattcaagct
     3361 ttaccgttcc tttcttttca tcgattttta ttaatgcata tccgggcaga tctgtctgtt
     3421 tgtaaaatgt cacaaattcg gcttcctcag caagaattgc tttccttgat tcaattgtgg
     3481 agggttccca gtccggtaca ttttctgcca gcgaaggacc atattcggta atcagcttat
     3541 ccggtttaaa ataatctctg tcactaacta cacttattgc catcacctgt acgaccggac
     3601 cataatcggt gtttctcctc accactgaat atctgtgcag gtgagcacat aaaacaattg
     3661 ccttgttacg tgctattact tcgagcaatt tttccctccg gaccggatcc cgccttagtg
     3721 tatgccagca cctctcggtg accggtatta caggttcatg gattgcaaca aatttaacaa
     3781 cagcgtcaga tgaagagagt tcttcgtcca ggaatttaac catatcggta cttttgtccc
     3841 atggatctat acaggtgatc tgaatatttc cgtgacgata agtatagctg gcattcatta
     3901 tcccggaatt tccggtttgc ttccttaaca tcggggtata atgactgaga aaggcatcta
     3961 ctgctccagg cccggtaata tcatgatttc cttttgtaat tatccagggc acccctgttt
     4021 ccgcagtttc aactgccctg actatttccc gggccatctg atcagctttc tcttccgttc
     4081 ctgccagtcc ttctgaaaga tctcccagct gtataagtgc tttgacctga ggtcttacat
     4141 ttaaaacctt atttcttaaa atcgacatga agtcatccca gttattttca gtgatctcag
     4201 tgtattcctt tacctgtctc agatcatcgg gcttatccat cagccagttc atatcgtgta
     4261 acctgatatt gtcataatga agatcaccca ggactatgag actgcttttc tgccctgaag
     4321 aaagaagatc agtgacggcc agtaacaaaa aaagaattat cgttttccgc ataattttgt
     4381 aaaaaaccaa atttattaaa atccctgaag ttttagttat caaaaatatt aaggagctct
     4441 tttgttatgc tgcatgatca ggagcataaa gttaaaagcc ttccgtaata aaatccatat
     4501 atttgtctga aaaatccata aagcgtatca atgaaaaact cccggatact tgcaataata
     4561 acaggattta tattctcctg ttccctgata tccttctccc agtccggaaa cgttttaaag
     4621 atcttccagt ttccggctga cagaatacca agaatagatg gtaattcgga tgactgggat
     4681 attgtacctg ctggttatga aattggcagc tacctactca gggatgatga aaagaaacat
     4741 tcggcaccgg atcccgctaa cctggatgta aaggtgaaag tgggctgggt aaaaggcatg
     4801 aataagttgt atttccttta tgaggcttat gataattact gggatttctc aaaggttggc
     4861 cttcatcatg atatatttga aatagtagtc gatgctgacc agtccggggg gcctttaata
     4921 gagcagttcc atcctaatcc agatctgaac aatacatggg aagcatattt taattttcac
     4981 ggagttcatg cacagaacta ccatatcttt actccggctg aaggtgagga ctggtgcctt
     5041 gcatggggca gccagccatg gataaaagag ctgccctatg caaatgctgc ttataaatat
     5101 gatttcaggc cgggagaatc gggaaaattg gttcttgaat tctggataac acctttcgat
     5161 tatgccggac ccgagggtcc tgtcagggca gtcgaatcag tgctggaaga aggaagggac
     5221 ataggcttgt gctgggcagt tattgatttt gatgatgtca atgccagaag taataacggt
     5281 ttctggaact tatcggaaca tcatacaatg tatgggaacg cctcgcagct gagaaccttc
     5341 cgcttaatgc ccctggaaca gcaattcagg aaaaagatcg acgctcagtg gtcattcagg
     5401 atcatagata tggacaggag aatggttgct ttccgggatc agtcggcagg acagataaac
     5461 tcctggaaat gggatttcgg agatgggaca acctcatccg atcaacatcc ggtccatacg
     5521 tacacaaagc cgggaagata tattgtcata ctgtatatag aggggcctga tgggaaatcg
     5581 agaatgggaa agatgtggga tgtggcggtg agatcaggga gactggacag ggatgttaag
     5641 tcgaattgat cggaaaggat tgcttcgttg tcccgtttgc atacggtgta ggtttaccga
     5701 aggcttaggg cgggactcct cgcaaggacg gtaacacaga tgatggccag gtagttatcg
     5761 gaaaggattg cttcgtcgtc ccgtttgcat gcgctgcagg tttaccggag gcttagggcg
     5821 ggactcctcg caatgacggt aacgtggatg atggcatggt aattatcggg aaggattgct
     5881 tcgtcgtccc gccgagggcg ggcctcctcg caatgacggt agtattaatg taactaattg
     5941 caatatgaga ctaaaaagta ttattctggt aattatggcg gggatgagta tacctgctgt
     6001 ttcgcagctg aaagtgacgg ataccaatac tccgcttcat gccatgcagc ctgactatcc
     6061 ggtggcatac ggacctgcaa aggctgagga tattaaagcg gttttaaatc gtttgtttaa
     6121 ttaccttgac gaatcgacgc cggcgaaact tgttgatcca cagacaaagg cagagattat
     6181 ggacctgaaa aaaatgacac cggggactgt tttcggacag ggagtattcc ggctgataag
     6241 ttatgaatgg ggtgtcacct atggagccat gcttcttgcc gcagatgcga caggcgatcc
     6301 gaaattcagg gactatacca caaaaaggat ggagctgata tctgccgttt cacgttatta
     6361 taaggaattg cctcccgaca ggcaaaagaa caatccggtc cgatccgttc ttgaacccag
     6421 agctctcgat gatgcaggat cgatgtgcgc agcaatgata aaaacacttg gatcgggaaa
     6481 taatccttca ttacgtccat tcattgacaa ctacatagat tatatttcat caaagcagca
     6541 gagactggca gacggaacct tgtcgcgaaa caggcctctt ccggatgcct tatggcttga
     6601 tgatctttat atgagtgtcc ctgctctggc acagatgggg aaaatgacag gcgacaggaa
     6661 atactttgac gatgcctgca aacaaataat ccagttttca caaaggatgt tcaatgctga
     6721 aaaaggactt ttcatgcacg gatggataca gggcatgaat cctcatccgg aattttactg
     6781 gggtcgatgc aacggatggg cccttctggc aatgtcagag cttctcgatg tcctgccggc
     6841 tgatcatccc tctgtaaaaa caatactgga gcttttccgt tctcatgcag ccggacttgc
     6901 ctcttaccag tctgacaccg gattctggca tcagcttatt gatcgaaatg attcgtacga
     6961 agaaacatcg gcaacagcaa tattcactta ctgcttttct cacggcataa ataagggatg
     7021 gctcgatgca agggcattcg gcccggcagc agtattggga tggaatgccg tacagacaaa
     7081 agtgaacgat ctgggtcagg ttgaaggaac ctgtgtggga acaggaatgg ctttcgatcc
     7141 tgcattctat tatcaccggc ctgtaaacgt agctgctgca cacggttacg gcccggtaat
     7201 aatggccggc gcggaaatcc ttacattgat gaagaactat cagatagtaa taaacgattc
     7261 agctgtaatg ttctatcctg caggaacaga ctggaagagc tacaggtaga ttatgacatc
     7321 cggaggatgg aaaatttcct tatggatctg atagttaatg aggaagatgc gagatcttct
     7381 caaaggatgg tggatttgtt ttcgcaccta tccataatat attatccgag gttccacctc
     7441 aaaacattgt tgccgcatat aatgccgtaa aagaattcaa cggatcgtag ggacatgccc
     7501 gtcctttacc tgtgataaaa tggttggttg atcgtaaggc aacgccatgg aatggttatg
     7561 taaccatggt tggtcaatgg tagggccacg ccatggcgtg
//
LOCUS       DAOOYG010000132         7579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10944_length_7579_cov_6.026715, whole genome shotgun sequence.
ACCESSION   DAOOYG010000132 DAOOYG010000000
VERSION     DAOOYG010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10944_length_7579_cov_6.026715"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PK288_09815"
     CDS             complement(<1..656)
                     /locus_tag="PK288_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPM19262.1"
                     /translation="MQIRPLRVQDLDFACALSEAEGWNQTERDWKFLLSGKNNVCLIA
                     DEGGRLVGTATALFHPPGLAWIGMVLVDRQMRGQGIGRMLMSEIIKRLDHCKSLKLDA
                     TPAGLSLYRKLGFKDEFTIYRMTNSSLHENQGSSLDFALRIDKNNIINVLQSDKRIFG
                     SDRSILLNYLMENEPRRAYVVLDADNAGGYIFGRTGLRYSYIGPACGISLHHVIKLIA
                     G"
     gene            complement(702..1760)
                     /locus_tag="PK288_09820"
     CDS             complement(702..1760)
                     /locus_tag="PK288_09820"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPM19263.1"
                     /translation="MNNHDAIADVLKRGVVIPALPLALNKNRKLDERRQRALIRYYLD
                     AGAGGIAVAVHTTQFAIRSPEVNLFLPLLELAREEFDRFLQSNEKPIIRIAGAIGKTR
                     QAIREASAARDHGFHCVLLSLAAFQGSSNKEILNHCRKISEIIPLVGFYLQPAVGGRQ
                     LDVNFWREFARIPDVVAIKIAPFNRYYTFDVVRGVAESGRADEIALYTGNDDSILTDL
                     LSEYRITAGDRVINKRIVGGLLGHWAVWTKSAVDLLEAVHNGKFDNDVQNASVLANKI
                     TDCNSAFFDSANNFKGCIVGLHEVLRRQGLFEGIWTIDRNERLSPGQKAEIERVYRSY
                     PELNDDDFVAANLDRWMA"
     gene            complement(1785..2822)
                     /locus_tag="PK288_09825"
     CDS             complement(1785..2822)
                     /locus_tag="PK288_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epimerase"
                     /protein_id="HPM19264.1"
                     /translation="MIDLPEKISSVEQLEELISRPYDETVKLFTSLDGDILILGINGK
                     IGPSLARMARRASDLAGSGKNIIGVDRNFDPVQRSVFKALNIEIIEGDLLDVSFVNSL
                     PGAGNIFFLAGMKFGAVDNLPLTWAINSYLPAIVAEKFKNSTIVAFSTGCVYPLVPID
                     SGGSLESDPPVAIGEYAQSCLGRERMFEYGSLRYNTKTALIRLNYSVEMRYGVLVDIA
                     TRVQNRQPVDLSMGHFNVIWQGDVNNYVLQSLKIAATPAKILNLTGPEILSVRDVATE
                     FGNLMGIKPQFVSSESDTALLSNSSDAYTLLGSPKVDISRVIRWVAGWLMERRPLLGK
                     PTHFEVRDGKY"
     gene            2973..5918
                     /locus_tag="PK288_09830"
     CDS             2973..5918
                     /locus_tag="PK288_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPM19265.1"
                     /translation="MAMKMLNDVVKPGFHKVVNPEALSAFEEMMPENSLLVVLVAGKG
                     TRFGKEPKCIQMVQGTPLARHSIDAFRRFSPSPVICIVGYRYIDVVEALGNDNVYVLS
                     DNPAGGTGYAVFESFSVPDLVTKNPLLIITMGDRVIPSSVFRKIEKAHTEGDREADLT
                     FMSAVYEPPKNSGKGRVIRDESGNVLRIMEERDIMREENQYTRQALLGITEGNCPLYI
                     IRASTLFDHLSGISNNTAQGQYYLTDIVQSIAERGGEIRTITTRLSDPEYDLLTSDVT
                     QPIDLAMLEGLLSSNLGLLVPEEMEIERAAGDIIADKPAGQVRSITRQIGDLLTMIKR
                     EKLPFEPDKPVGIGISGGRLRIAFMHPDMVRFYGPAWQMPIGASSANGDEQIVVIVQT
                     ADDGRIHYVPFNQQYRESIDSIPSDFNTMYPDDEVSDINTYEKFGTRMSEELLLSLGY
                     FSDEELAERRRRNLPLPPSSLWISSNMRRPFALIGNAIASLRTLKTGSLGVKIRETLG
                     QRNFKGLHIVSAGNIPQGGFSSSSAVTVAVKNAINSLYEVGITPDLLVHLACQAEYGT
                     GVRAGSLDQATEQKGSAGKGTLISSNPKDNYRILGTFPVPDTRYQIIFPYSVERDRNA
                     WKWSWGAYAEKSGDGCLTTTEMRKLTGKAAEIAALLVRLPLETDFFKHIEDDLLADGE
                     LNDENTSWICSLLLRLPLLITKEELACRLAQNHAWLTAQIMEVYRLEQKNASFKADEI
                     IRSMLDGWNDPVLRRTDARGVIVKEKGVPLRAIVAYLFAEVAKNFYLIYHPESWIECV
                     THSQRGDCSVSIDFKKLPGKTEMENRLGWENGAVGPDLLNVWLEKFDAKPFDFNAGLD
                     DMSLAGPEKPVFRKLSGSSFFRGLALIDLAEAMLKKAFGDDSVAVRINAAGQGDYFQV
                     HIDMQKIDPDRVKQFIRKAIYRRFGLKPNPDFVEVSSGGGASGIRLSRFDFLSQLMMK
                     LNSLVPTGV"
     gene            5999..7435
                     /locus_tag="PK288_09835"
     CDS             5999..7435
                     /locus_tag="PK288_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPM19266.1"
                     /translation="MKSSIITFLSAVFLLCSASWLNGQERYRIDNIVRALKAKDCMTA
                     VELLPEVKDINFRDAYGGTLLMHASLNGCNELVKALLERGAKTDLRSNDGFTALHMAS
                     RNGYPEIVKVLLAKGADVNILTNKKATALYLAAQNGHTDIIKLLYEKGSNIDALAEGE
                     FSPLFIASQNGHFDAVRFLAENSADLEFSTSRGATALFIASTSGNLEIVEYLLQKGAN
                     IEAKTSRGATPLFTASQEGQSRIVKLLLEKGADINMKTNDGMTPLAAATRRDHLETVK
                     ELLARGADPDIRSEQGFAPIHMAAELGYAEIVRLLLRKGTIDLRANNGATALMYAAKE
                     GKVETAGLLLDNGADANIQSSDGYSALYVAVQNGHTEIVNMLLKKVKNPDMMTSDGFT
                     PLMIASQKGSAEIAAMLVDRGANINLQNNEGATALMIASAYGFSHIVKLLLDNGADIS
                     RKTNDGKAAVDLALNDDIKNMILNAKKD"
BASE COUNT         2123 a         1710 c         1731 g         2015 t
ORIGIN      
        1 ccggctataa gcttaattac atggtgtaaa gatattccgc atgcaggacc gatataggaa
       61 tacctcaaac ccgtcctgcc aaatatgtat cctccggcat tatctgcatc cagtaccaca
      121 taggctcgcc ggggttcatt ttccataaga tagttcagga ggattgagcg gtcggaacca
      181 aatattctct tgtctgattg caaaacattt ataatattgt ttttgtcaat tctcagggca
      241 aaatccagac tgctcccctg attttcatgc aaggaggaat ttgtcatccg gtaaatggta
      301 aattcatctt tgaagccaag cttccggtaa agggataatc ctgcaggagt cgcatccagt
      361 ttgagggatt tgcaatgatc aagccttttt attatttccg acataagcat cctgccaatc
      421 ccttgccccc tcatttgcct gtcgacaagc accatgccga tccatgccag gccgggggga
      481 tgaaaaagcg cagtggctgt accgactaac ctgccgccct catcagcaat aaggcagaca
      541 ttgttttttc ccgacagcag gaatttccag tccctttctg tctgattcca gccttcagcc
      601 tcagaaagag cacaggcaaa atccagatcc tgcaccctca gtggtcgtat ttgcatttta
      661 tttttacggt atagagtccc gaataaggct atgtgctatt tttatgccat ccagcgatcg
      721 agattggcag ctacaaaatc atcatcattt agttcgggat atgatctgta tactctttcg
      781 atctcagctt tctgacccgg ggaaagtctt tcattcctgt caatggtcca gatgccttca
      841 aataaaccct gtctccggag aacctcatgc aatccgacaa tgcatccctt gaaattattg
      901 gcggaatcga aaaaagctga attgcagtcg gtaatcttat tggccagtac ggaggcattc
      961 tgcacatcat tgtcaaattt gccgttatgc actgcctcca gcaggtcgac tgctgatttt
     1021 gtccatacag cccaatgccc aagcaagcct cccacaatcc gtttgtttat taccctgtct
     1081 cctgcagtta tccggtattc tgacagaaga tcagtcagaa tgctgtcatc atttcccgta
     1141 tacaatgcaa tttcgtctgc cctgcctgat tctgcaactc ctcttacaac atcgaaggta
     1201 tagtacctgt tgaatggagc aatctttatt gcaaccacat ccgggattct ggcaaattcc
     1261 ctccagaaat tcacatcaag ctgccttcca cctacagccg gctgcaaata aaacccgaca
     1321 agcggaataa tctccgatat tttccgacag tgattcagga tttctttatt tgatgaaccc
     1381 tgaaatgcag ccaggctcag aaggacacaa tggaaaccat gatcacgtgc agcgcttgcc
     1441 tccctgatgg cctgtctggt tttcccgatg gcccctgcta tcctgatgat gggtttttcg
     1501 ttgctctgta aaaaacgatc aaattcttcc cttgcaagct caaggagagg cagaaaaaga
     1561 tttacctcag gggatctgat ggcaaactgg gtggtgtgaa cagcaactgc aatcccaccg
     1621 gccccggcat caagataata tctgattagg gccctctgac gcctttcatc cagtttgcgg
     1681 tttttgttaa gtgccagcgg aagtgccgga attacaacac ctcttttcag aacatcagca
     1741 atagcgtcat gattattcat aggtttcggt ggtttaactg gttattaata ttttccgtcc
     1801 ctgacctcaa aatgggtggg cttgccaagc aatggtcttc tttccatcag ccagccggct
     1861 acccatctga taaccctgga tatatcaacc tttggtgatc caagtagcgt atacgcatcg
     1921 gatgagttgc tcagcagggc agtatccgat tcactgctga caaactgagg cttaataccc
     1981 ataagattac cgaattcagt tgcaacatcg cgaaccgata aaatttccgg acctgtaaga
     2041 ttaagaatct tggcaggagt ggcggcaatt ttgagtgact gcagaacata attattcaca
     2101 tcgccctgcc atattacatt aaaatgcccc atgctaaggt caaccggttg tcgattctga
     2161 accctggtgg caatatcaac aagcacgcca tagcgcattt ccacagagta attgagcctt
     2221 ataagggcag tttttgtgtt gtatcttagg ctgccatact cgaacatcct ttcccggccc
     2281 aggcatgact gtgcatattc accgattgcc acgggaggat cactctcaag cgaaccaccg
     2341 gaatcaattg gaacaagagg gtatacacag cccgttgaaa atgcgacaat agtggaattt
     2401 ttaaatttct cggctacaat agctggcaga tatgaattta ttgcccatgt caggggaaga
     2461 ttatcaacag ctccgaattt cattccggcg aggaaaaata tattcccggc accaggaaga
     2521 ctgttgacga aactaacatc aagcaggtca ccttcaataa tctcaatatt cagtgccttg
     2581 aaaactgacc gctgtacagg atcaaagttt cggtcgacgc cgataatgtt tttcccggaa
     2641 cccgccaggt cacttgccct tctggccatt cttgcaagcg acggtcctat tttaccgttt
     2701 attccaagaa taagaatatc tccgtccaga cttgtgaata atttcacagt ttcatcataa
     2761 gggcgcgaaa taagctcttc cagctgttcc acgctgctga ttttttcggg taagtcaatc
     2821 atgctgcatc tgttaaaaag taaacctgca aggtaacaat ttgaattcat ttgtatgtca
     2881 atttccgaca ttttgaactt ccgcgagtaa aaaatcgcag cactttaatt agaaggaatt
     2941 atatttaaca ctacttttct aacctgtcta tcatggctat gaaaatgcta aacgatgttg
     3001 taaaaccagg ttttcataag gtagttaatc cggaggccct ttcagccttt gaggaaatga
     3061 tgccggagaa ttcccttctg gtagttcttg ttgcaggaaa gggtacacgc ttcggcaagg
     3121 agccaaaatg cattcaaatg gttcagggaa cgccccttgc ccgtcattca atcgacgcct
     3181 ttcgccgttt ttcaccctca cctgtgattt gtattgtagg atacaggtat attgatgtgg
     3241 ttgaagcatt ggggaacgat aatgtttatg tgctttccga caatcctgcg ggagggaccg
     3301 gatatgcagt ttttgaatcc ttcagtgtgc cggatctcgt taccaaaaat cccctgttaa
     3361 ttattaccat gggcgacagg gtgatcccct cttcagtctt ccgtaagata gaaaaagctc
     3421 acaccgaagg cgacagggaa gctgacctga cttttatgag tgctgtttat gagccgccaa
     3481 agaattcagg caaaggcagg gttatcaggg atgaaagcgg taacgtactt cgcataatgg
     3541 aagaaaggga tattatgcgg gaggaaaatc agtataccag acaagccctg cttggtatta
     3601 ctgaaggtaa ctgccccctg tatattatca gggcatcaac tttgtttgat catctgtcgg
     3661 gaatttcaaa caatactgcc cagggacaat attacctcac agatatagtc cagtcaattg
     3721 ctgaacgggg aggtgaaata agaaccatca ccaccaggct ttctgatccc gaatatgacc
     3781 tgctcacctc tgatgtcact cagcctattg atcttgcaat gctggaagga ttgctttctt
     3841 ccaatcttgg actgcttgtt cccgaagaaa tggagattga aagggcggcc ggtgacataa
     3901 ttgctgataa accggctggc caggtgagat caattaccag gcagatcgga gatttactca
     3961 ctatgataaa aagggaaaaa ctgccgttcg aacctgataa gcccgtcggc atcggaatct
     4021 caggcggacg tcttagaatt gcctttatgc acccggatat ggttaggttc tacggacctg
     4081 cctggcaaat gccgataggc gcaagctccg ccaatggcga cgaacagata gttgtcattg
     4141 tgcaaaccgc tgatgacgga agaattcatt atgtaccttt taatcagcaa taccgggaaa
     4201 gtattgattc cataccgtca gactttaata ccatgtatcc ggacgatgaa gtttccgata
     4261 ttaatactta tgaaaaattc ggaaccagga tgagtgaaga gcttctgctt tcactgggat
     4321 atttcagtga tgaggaactg gcagaaagga gaaggagaaa ccttcccctg cctccgtcat
     4381 cactttggat atcaagtaac atgaggcgtc cgtttgcact tatcggaaat gcgattgcct
     4441 cattgcgaac actaaagacg ggcagtctgg gcgtgaaaat cagggaaaca cttggccagc
     4501 gtaattttaa gggattacat atcgtttcag ccgggaacat tccgcaggga ggattttcca
     4561 gctcctccgc agttaccgtt gcggttaaga atgcaataaa ctcactctat gaagttggta
     4621 taactcccga tttgcttgtc caccttgcct gtcaggctga atatggcaca ggggtaaggg
     4681 caggctcact cgatcaggct acagagcaaa aaggaagcgc aggtaaggga accctgatct
     4741 cttccaatcc caaggataat tacaggatcc ttggtacatt tccggtaccg gacaccaggt
     4801 accagatcat ttttccctac agcgtagaaa gggatcgcaa cgcatggaaa tggtcatggg
     4861 gagcttatgc ggagaaatca ggagacggat gcctgacaac aacggaaatg cgcaaactca
     4921 cgggtaaagc tgctgaaatt gccgcattac ttgtaaggct tccccttgaa acggatttct
     4981 ttaaacatat tgaggatgat ctccttgccg acggtgagct gaatgatgaa aatacatcat
     5041 ggatttgctc acttctactt aggctcccat tgctgattac aaaagaagaa ttagcctgca
     5101 ggctggctca aaatcatgca tggctcactg cccagataat ggaggtatac cgtctcgaac
     5161 aaaaaaatgc ttcatttaaa gcagacgaaa tcatcagatc gatgctcgac ggatggaatg
     5221 accctgtatt gcgccggact gatgcccggg gagtaattgt taaggaaaaa ggagtaccgc
     5281 tcagggcaat tgtagcttat ctcttcgctg aggttgcaaa gaatttctat ctgatctacc
     5341 acccggaaag ctggatcgaa tgtgtcactc attcccaaag gggtgactgc agcgtgagca
     5401 tcgattttaa aaagctgccc gggaaaactg aaatggaaaa tcgcctcggc tgggagaatg
     5461 gtgcggttgg gcccgatctg ctgaatgtat ggcttgaaaa attcgatgca aagccgtttg
     5521 actttaacgc cgggctggat gatatgtcac ttgccgggcc ggaaaagcct gttttcagaa
     5581 agttgtcagg ctcgagtttt ttcaggggcc ttgcacttat agatctcgct gaagccatgc
     5641 tgaaaaaagc ctttggcgat gattcagttg ctgtcagaat aaatgcggcc ggacagggag
     5701 attatttcca ggtacacatt gacatgcaaa aaattgatcc cgacagggtc aagcaattta
     5761 tcaggaaggc catttatcgc cggtttggac ttaaacccaa tcctgatttt gttgaagtaa
     5821 gctccggggg aggagcaagc ggaatcaggt tgagccggtt tgacttttta tcccagctga
     5881 tgatgaagct gaactccctt gttccgaccg gggtctgaga ggatattata ttgatattct
     5941 gaacatccgg tttgtttttt ttgtttaatt taggatttgt gaaaaatatg agattagcat
     6001 gaaatcctca attattacat tcttgtctgc cgttttcctt ctttgttccg catcttggct
     6061 gaacggccag gaaagatacc ggatcgacaa cattgtaagg gccttaaagg ccaaagactg
     6121 tatgaccgca gttgaacttc tccctgaagt aaaagatatt aacttcaggg atgcctatgg
     6181 ggggaccctt ctgatgcatg cttccctgaa cggatgtaat gagctggtga aagctttgct
     6241 tgaacgcgga gcaaaaacag atctgagatc caatgacggt tttactgcct tgcatatggc
     6301 ttcgcgcaac ggctatcctg aaattgtcaa agttctgctt gcaaaaggcg cagatgttaa
     6361 tatccttacc aacaagaaag ctaccgcact ttatctggcg gctcagaatg gacatacgga
     6421 tattattaag cttctgtatg aaaaaggcag taatattgat gcacttgctg aaggagaatt
     6481 ctctccattg tttatcgcct cacaaaacgg gcattttgat gctgtcagat tcctggcaga
     6541 aaactctgct gatctcgaat tttcaacctc ccgcggcgct actgcacttt ttattgcttc
     6601 caccagcgga aatcttgaaa ttgtcgaata tcttctgcaa aagggagcca atatcgaagc
     6661 aaaaaccagc agaggagcaa ctcccttatt tacggcatca caggaagggc aatcacggat
     6721 tgtaaaatta ttgctggaaa aaggtgcaga cataaatatg aaaacaaacg atggaatgac
     6781 tcccctggca gcagcaacaa gaagggatca tcttgaaacg gttaaggaat tgctggctcg
     6841 aggagctgac ccggatataa ggtcggagca gggatttgcg ccgattcaca tggcagccga
     6901 actgggatat gcagaaattg tcaggctgct gctcagaaaa ggcacaattg atttaagggc
     6961 aaacaacgga gcaacagccc ttatgtacgc tgcaaaagaa gggaaggtgg aaactgcagg
     7021 gctgctgctt gacaatggag cggatgccaa tattcagagt tccgacggtt attcggcctt
     7081 gtatgttgct gtgcagaacg gacatacgga gattgtgaac atgttgctga aaaaagtgaa
     7141 aaaccccgat atgatgactt ctgatggctt tactccgtta atgattgcat ctcaaaaagg
     7201 atcggcagaa atagcggcaa tgcttgtgga tcgcggagcc aatataaatc ttcagaataa
     7261 tgaaggtgca actgccttga tgattgcatc ggcatatggt ttttcacata ttgtaaaact
     7321 tctgctcgat aacggtgccg acatctcccg gaaaacaaac gacggaaagg cagcagtgga
     7381 tcttgccctt aatgacgata taaaaaatat gatcctgaat gccaaaaaag attaagatga
     7441 catataagga ttttttcatt ataaaaaaaa gcagctatga aattcattca taactgcttt
     7501 gctataatgt gatcccgaca ggagtcaaac ctgtaacctt ctgatccgta gtcagatgct
     7561 ctattcagtt aagctacgg
//
LOCUS       DAOOYG010000133         7565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_10969_length_7565_cov_7.104394, whole genome shotgun sequence.
ACCESSION   DAOOYG010000133 DAOOYG010000000
VERSION     DAOOYG010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7565
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10969_length_7565_cov_7.104394"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PK288_09840"
     CDS             <1..722
                     /locus_tag="PK288_09840"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM19267.1"
                     /translation="SLNGIFYKNTQSANEELYGEISSDSRFRERFIPFAIINPIYAGW
                     KDDFEISVNRLGMKGLRLFPQYHDYEITDPALIELVKMARDRGLPVAFDIRMVDSRQK
                     SWLDLPHYDFCAPVKSDIIFREWSLRNVLPVIKAVPDAKYIIVNLANSISLDAGETDI
                     IKRTDLVFDTSGRSIRDNNDLALLLKRFGRERFAFGTHSPILDYLTGRLRIESMSDDE
                     ADENTKELIRSGNAKRILGLA"
     gene            935..2593
                     /locus_tag="PK288_09845"
     CDS             935..2593
                     /locus_tag="PK288_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19268.1"
                     /translation="MMMKNLLQKSAVIGITVALLSSVSSYAQSLWKTALNNKDVLVLS
                     TLFTAQDCRDKINTAEGLDAAVKWCKETGLTRVFIESFRGGYYAEKETLVNARDRFRK
                     EGFDVAGCVTTVRVGKNGVGGWGMTPCYTHESTVKEFEKIFEYTASIFDLIMIDDFLF
                     TECTCEDCIAAKGDLTWAKFRSDLLVKFSNDHIIRPAKRVNPKAEIIIKYPLWYDYFH
                     ERGYEVVRESMDYDYTWVGTETRDYNFDIKPGGTVQYNAFFIMRWLDGIGAGKAGGGW
                     IDALGTTPKYYVEQARQTVLGNGREIMLFHYGSLQTNSNTYDGWDGTPIADVDALKKE
                     IPLLFDLARLVRGKALKGIYLPKLPNSEPIKEPYIFSYLGMLGLPLIPGHELTSSDPS
                     VIYTTHALKDPSFSSKLYSVLNSGKQVAVTEELANRLSDQTLLENKNLMVFKSGDDPK
                     KFLKMTKEEIQPLRDRLLAPLGIKFNAPVKVSFYLLGDSHIVIENFNDEPVDATIELP
                     KVTDARQALLIPSGGKAALSKNGNVVTLKGISPRTLVAFEYRQQ"
     gene            2695..4011
                     /locus_tag="PK288_09850"
     CDS             2695..4011
                     /locus_tag="PK288_09850"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPM19269.1"
                     /translation="MKFPLSLTCVFIVAFMASCGVSPGKKKQKELARMIVNDEYFAFT
                     KKKALEVMKTGFNAGDGYREVWIRDYNTFIKLASEVFPGEDLKENLLVFFRMQGADGN
                     IIDGFTPAEQISGDKSDFIYSELEPRYAGHKNTVETDQESSLVQAVYKYVKTTGDRAF
                     LDEEVNKKTVAERLTDAMDFLLNHRFSREYGLIIGATTADWGDVQPEHGWGVDIDENT
                     HPAIDIYDNAMFIIALDNLAELVPATAEKFGNIRDSLAHNCRKYLWDASAGKFRPHIY
                     LDRGSPFPPDFDENQIYYFGGTAIAIEAGLLSRDEIKMSLDEMIRRVKLAGAATIGLT
                     LYPPYPEGFFANRIMNPEYSYQNGGDWTWFGGRMIQQLVVHGFVEEAYEQIAPMVKRV
                     KDNNGFYEWYSIDNQPRGSGTFRGEAGVLYDAINMLEKWAGKIEMM"
     gene            4225..7149
                     /locus_tag="PK288_09855"
     CDS             4225..7149
                     /locus_tag="PK288_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPM19270.1"
                     /translation="MRFSIFFLIVFITVLPSSFGQDYPSRQRPASSFPDSVRYEVRPY
                     YRHRQDGKPGREVMLYFPGNRFVGRGTLSVEYNGQSEKINIEKPEGIDSLAVLLPEGI
                     GVSSDDEVTVQLSSGRKKITTGIRVPRKRQWTVYIYPHSHVDIGYTNTHENVELIHKR
                     NLIYGIDLAKRTQNYPDGAKYLWNPEVLWPVERYLSKANERQKQAVIEAVQKGWLQLD
                     AGYVHTNTSAAADEELFEFFRICSRMKELTGKNIETLVQVDIPGMTWGIVPVAAAMGI
                     KYCLALNNGFDRVGLSTDLSFKPFWWKSQDGKSRILFFQPGSYNPGAIYKGFQYWPLM
                     AGHTDSTTLLKIVKSDNPRAFFVDKYLAEKLPMLENSNYYPYDIFLMTWAMADNTPID
                     ADLPEAVRSWNEEYAYPHLIIAGATQMMKAFDDKYGDSLPVLSGDFTEYWTDGLGTAA
                     KYTAMNRASKERLIQVETLWAMLNRDLPPPRREISEAWRNIILGTEHTWCFMDPQKQP
                     ITNDILKVKFGFFETAREMSSSLLDRSFERIRQENSRFIAVFNTLSWERDGIIYLAPD
                     QAGNYNSVIDDKGRQLSCQKLASGGLVFAAGRIPALGSVVYTLSKKKVRSAGAIAAGY
                     TLDNGIIRVTLDPRTGDISSLISSGYDYAESGSESLINSYRYLHGEDPPEKATSAKNV
                     RITLKENGPLLATLQVISDAEGCRSLVREITLYSGQPFVDIRNIVDKIAVTEKEGIHF
                     GFGFNVPSPAVRFDIPWGVTELEKDQLPEANRNWITFQRWLDISNSDRGVTFCSPDAP
                     LFQNGTITANILGGAANSPKWIRKLEPSSIIYSWALNNHWHTNFPLSQDGSIEFRYRL
                     LPHNSGYDAAVSNRFGLEQAQPLIACPAGKEIQVQAPLQFEGSRNIFLTILKVGETGK
                     SMILRFRSVSDSDEKITLNWPEDKQVSLRIAGPDERFIEMADDGQLIVPAKGFVTLEA
                     EW"
     gene            7335..>7565
                     /locus_tag="PK288_09860"
     CDS             7335..>7565
                     /locus_tag="PK288_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19271.1"
                     /translation="MRNSVTGGILSALFVLLTTSSGHAQNERLSFGKSVSLDKAIFST
                     KEKEQPLIWINVNTDRDTWHVEKDILVCSGKPI"
BASE COUNT         2170 a         1547 c         1902 g         1946 t
ORIGIN      
        1 ccagtcttaa tggtattttc tacaagaaca ctcaatcagc caatgaggaa ttgtacgggg
       61 aaatcagttc cgatagccgc ttccgggaaa gattcatccc ttttgccata ataaacccga
      121 tatatgccgg ctggaaagat gattttgaaa tatctgtaaa ccggctgggg atgaaaggac
      181 tgcgattgtt tccccagtac cacgactacg aaattaccga tcctgcgctt attgaacttg
      241 tgaaaatggc acgcgaccgg gggctgcctg tggcatttga tatcaggatg gtcgacagcc
      301 ggcagaaatc atggctcgat ctgccacatt atgatttctg tgccccggta aagtcagata
      361 ttattttcag ggaatggtca ttaagaaatg ttttaccggt cattaaagct gtcccggatg
      421 caaaatatat aatcgttaat ctcgcaaaca gtatttcact ggatgccgga gaaacggata
      481 tcataaaaag gactgatttg gtttttgaca catccggaag gtcaataagg gataataatg
      541 atctggccct gcttttaaaa cgctttggca gggaaaggtt tgcatttggg acccattctc
      601 cgatactcga ctatctcacg ggcaggctcc gcattgagtc gatgagtgat gatgaggctg
      661 atgaaaacac aaaagaactg atcagatcag gaaatgcaaa acggatactg ggcctggcat
      721 aagatcaggt gtcggcagat ccccgctttg caggtttcag gaaaattatt cagatagctg
      781 cctgcataga acacgattga gctattttat tcagccggga gaataatttt tgctgttcat
      841 tggtatatgc ggaaaaagcc ctgtttaagt attgtcccgg gagtcattaa aaggaataat
      901 atgcaactta ttaattcata aaccagtaaa ttcaatgatg atgaaaaatc tcttgcaaaa
      961 atcagctgtt attggaataa cagtggctct gctgagctct gtttccagtt atgcccagtc
     1021 attatggaag actgccctga acaacaagga tgttcttgta ttatcaaccc tgtttacggc
     1081 acaggattgc cgcgacaaga tcaacacagc tgaaggcctt gatgcagctg ttaaatggtg
     1141 taaggaaacc ggacttacca gggtttttat tgaaagcttc cggggtggat attatgctga
     1201 aaaagaaact cttgtcaatg caagggatcg ctttaggaaa gaaggatttg atgtggcagg
     1261 ttgcgtaact actgtgaggg taggaaagaa cggtgtgggt ggttggggga tgaccccgtg
     1321 ttatacccat gagagtacgg ttaaggagtt tgaaaagatc tttgaatata ccgcatcgat
     1381 atttgacctg ataatgatcg atgatttcct cttcaccgaa tgtacatgcg aagactgtat
     1441 tgccgcaaaa ggtgatctta cctgggctaa gttccgcagc gacctccttg tgaaattcag
     1501 caatgatcat atcatcaggc cggcaaaaag ggttaatccg aaagcggaaa tcattattaa
     1561 atatcctctg tggtacgatt atttccatga gcgtggctat gaggttgtaa gggagagcat
     1621 ggactatgac tatacctggg tgggtaccga gacccgtgat tacaatttcg atataaaacc
     1681 cgggggaact gttcagtaca atgccttttt tatcatgagg tggctcgacg gaatcggtgc
     1741 aggcaaagcc ggcggcggat ggatcgatgc tctcggtact acaccgaaat actatgtgga
     1801 gcaggcccgc cagactgttc tgggaaatgg cagggagatt atgcttttcc attacgggag
     1861 cctgcagaca aattcaaata catatgatgg ctgggatggg acgcccattg ccgatgttga
     1921 tgcacttaag aaagagatcc ctcttctgtt cgatcttgca cggcttgtca gaggcaaagc
     1981 cctgaagggt atttatctgc caaagctgcc aaacagtgag cccatcaaag agccgtatat
     2041 tttcagttac ctgggaatgc ttggcctgcc tctcatacct ggtcatgaac tgacaagcag
     2101 cgatccctcg gtgatctata caacacatgc tctcaaagac ccgtcgtttt cctccaagct
     2161 ttacagtgtt ctcaattcgg gaaagcaggt agcggtgacg gaagagctgg caaatcgact
     2221 gtcagatcag accctgcttg aaaataaaaa tctgatggta ttcaaatcgg gtgatgaccc
     2281 aaaaaaattc ctgaaaatga caaaagagga aatccaacct ctccgcgacc gcctgcttgc
     2341 tccgctggga attaaattca atgcccccgt taaagtctca ttctatcttt taggtgacag
     2401 tcatatcgtg attgaaaatt tcaatgatga acctgtggat gcaacaatcg aactgccaaa
     2461 ggttactgat gcacgtcagg ccttgttgat cccctcgggc ggaaaagcgg ctctttcaaa
     2521 aaacgggaat gttgtgaccc tgaaagggat ctcgccgaga acacttgttg cttttgaata
     2581 cagacagcaa taggaatatt gacgctcaaa ttcgataaat ttgcactgct ggcatgatat
     2641 gagtgttatt gctggcagta tatcatttaa ataaaaaact aattttctga gagtatgaaa
     2701 tttccgctaa gtcttacttg tgtttttatc gtggctttca tggcctcttg tggtgtttct
     2761 cccggaaaaa agaagcaaaa ggaacttgcc cgcatgatag tgaatgatga atatttcgct
     2821 ttcacaaaga aaaaggcact tgaggtaatg aagaccggat ttaatgccgg agacggttac
     2881 agggaagtat ggatccgcga ctataatact tttataaaac tggcttccga agtatttccc
     2941 ggggaagacc tgaaggagaa cctgctggtg ttcttccgca tgcagggagc agacgggaat
     3001 attattgacg gttttacgcc ggcggaacag ataagtggtg ataaatcgga ttttatttat
     3061 tctgaactcg aaccccggta cgcgggtcat aagaacactg tggaaactga ccaggaatcc
     3121 tcactggtcc aggctgtgta caaatatgtt aaaacaacag gagaccgcgc atttctggat
     3181 gaggaagtca ataaaaaaac tgttgccgaa cggctgactg atgccatgga ttttttgctg
     3241 aaccacagat tcagcaggga atacggactg ataatcggtg ccaccactgc cgattggggt
     3301 gatgttcagc cggaacatgg ctggggcgtg gatattgatg agaacaccca tcctgcaata
     3361 gatatttatg acaatgccat gtttatcatt gcgctggata atctggcaga actcgttccc
     3421 gcaacagcag aaaaatttgg taatataagg gattcacttg cacacaattg caggaaatat
     3481 ctctgggatg cttcagctgg taaattcagg cctcatattt acctcgacag gggctccccg
     3541 ttccctcccg actttgatga gaaccagatc tattatttcg gaggtactgc catagccatt
     3601 gaagccggac tcctcagccg ggatgagatc aaaatgtcgc tggatgaaat gataagaagg
     3661 gtgaagcttg ccggagcagc cactatcggg ttaacacttt atcctcctta tccggaggga
     3721 ttctttgcca acaggataat gaatccggag tacagttatc agaatggcgg agactggaca
     3781 tggttcgggg gaaggatgat acagcagctt gtagttcatg ggttcgttga ggaggcctat
     3841 gaacagatag caccgatggt gaaacgggtg aaagacaaca atggctttta tgagtggtat
     3901 tcaatcgata accagcccag gggatcggga acgttcaggg gtgaagcggg agttttgtat
     3961 gatgcgataa acatgcttga aaagtgggca ggtaaaattg aaatgatgtg attcaggcca
     4021 aaaaatgtcg gacaactttc tgacagaaat aaccaaagaa gccggggttc agtgaaaaag
     4081 aaacatagtt tgtaactgac cggaactgat cagaaaggat gtgaggaatg agaaacaagc
     4141 attatatgag gcgtttgaaa tgtgttggat aatgtacccg gtaaaatccg gtataatcct
     4201 gcaaattcag attatattat gaatatgaga ttttcgattt tttttctgat tgtttttata
     4261 acggttttgc catcatcatt cgggcaggat tacccgagcc gccaaaggcc ggcatcttct
     4321 tttccggatt cagtcaggta tgaggttaga ccctactatc ggcatcgcca ggatggaaaa
     4381 cccgggaggg aggtgatgct gtattttccg gggaacagat ttgttggccg gggtacgttg
     4441 tcggtcgaat ataacggcca gtcagaaaaa ataaatattg aaaaacctga gggtattgat
     4501 agtctggcag ttctgcttcc cgaaggtatt ggtgtaagtt cggatgatga ggtaacggtg
     4561 caactgagca gcggcaggaa aaaaataaca accggcatca gagtgccccg taaacggcag
     4621 tggaccgtat acatctaccc tcattcacac gtggatattg gatatacaaa tacccatgaa
     4681 aatgtggagc taattcacaa acgaaacctg atatacggta ttgatctcgc aaaaagaacc
     4741 caaaactacc ctgatggtgc aaaatatctc tggaaccctg aggtactctg gcctgttgag
     4801 aggtatctga gtaaagcaaa tgaaagacaa aaacaggctg tcatagaggc tgttcagaaa
     4861 ggctggctcc aactggatgc cggctatgta cataccaata catccgctgc ggcagacgag
     4921 gagctctttg agtttttcag gatttgttcc cggatgaaag aactgacggg gaaaaatata
     4981 gagactctgg ttcaggttga catcccggga atgacttggg gaattgttcc cgttgcagca
     5041 gccatgggaa taaagtattg ccttgcattg aataatggat tcgacagggt gggattgtca
     5101 accgatttaa gtttcaaacc cttctggtgg aaaagccagg atgggaaatc caggattctt
     5161 ttctttcagc cgggaagtta taatcccggt gccatttaca agggattcca gtactggcca
     5221 ttgatggccg gacatactga cagtaccacg cttctgaaga tcgtaaaatc ggataatccc
     5281 cgggctttct ttgttgataa gtatctcgcg gaaaaacttc ccatgcttga gaactcgaat
     5341 tattatccct atgatatttt tctcatgacc tgggcaatgg cagacaatac tccgatagat
     5401 gcagatcttc ccgaagctgt cagaagctgg aatgaagaat atgcatatcc tcatcttata
     5461 atagccggtg caacccagat gatgaaggcc tttgatgata agtatggaga cagtcttccc
     5521 gttctgtcgg gcgattttac ggaatactgg accgacggac tggggacggc ggcaaaatat
     5581 accgctatga acagggcttc gaaggaaagg ctcatacagg ttgaaaccct ttgggctatg
     5641 cttaacagag acctgccgcc cccgcgaagg gagatctcag aggcctggcg gaatataatt
     5701 ctgggtacag aacacacctg gtgcttcatg gatccgcaga agcagcccat aaccaatgac
     5761 atactgaaag ttaaattcgg attttttgaa acagcacggg aaatgagttc atcccttctt
     5821 gacaggtcgt tcgaacgaat caggcaggag aacagcaggt ttattgctgt ttttaataca
     5881 ctttcatggg aacgggatgg aataatatat cttgccccgg atcaggccgg gaattacaat
     5941 tccgtaatcg atgataaagg ccggcaactt tcatgtcaga agcttgcatc cggcggactg
     6001 gtttttgcgg caggcaggat ccctgccctt ggttcagtcg tgtacactct cagtaaaaag
     6061 aaagtaagga gtgccggcgc cattgcagcg ggctataccc ttgataacgg cataatcaga
     6121 gtaacactcg atccccggac aggagatata tcgagcctca tcagttcggg gtatgattat
     6181 gccgaaagtg gttcagaaag cctcattaac agttaccggt acctgcatgg agaggatccg
     6241 ccggaaaagg caacgtctgc taagaatgtc aggataactt taaaggagaa tggtccgttg
     6301 cttgcgacac ttcaggttat atcggatgca gaaggttgca ggagccttgt ccgcgaaatc
     6361 accctttatt ccgggcagcc ttttgtggat atcagaaata ttgttgacaa gatagcggta
     6421 accgaaaaag aagggattca ctttggtttt ggattcaatg ttccttcgcc ggctgtcagg
     6481 ttcgacattc cctggggagt gaccgaactg gaaaaggatc agctgccgga agcaaacagg
     6541 aactggatta ctttccagcg ctggctggac atttccaaca gtgacagggg agtgacgttc
     6601 tgttctcccg atgctcccct gttccagaat ggaaccatta ctgcaaatat tctgggcgga
     6661 gctgccaatt ccccgaaatg gatacgaaaa ttggaaccct catccataat atactcctgg
     6721 gctcttaata atcactggca caccaacttt ccgctgagcc aggatggatc gattgaattc
     6781 cgttacaggt tactgcctca taattcagga tatgatgccg cggtttcgaa caggtttgga
     6841 ctggaacagg ctcagccact cattgcatgc ccggcaggaa aggaaatcca ggtccaggca
     6901 cccctgcaat ttgaaggtag caggaatatt tttcttacca tattgaaagt cggggagacg
     6961 gggaagtcaa tgattttaag attccgttcc gtgtcagaca gcgatgagaa aattaccctg
     7021 aactggccag aggataaaca ggtatcactt agaattgcag gaccggacga aaggtttatt
     7081 gagatggcag atgacggtca gctcattgtt cctgccaaag ggtttgtaac actggaagca
     7141 gaatggtgac ctgatgcaga gcccgcctca atacaggtga cggatggttt ttgggtcttt
     7201 gcaggtgaat aatgacatac ccgttttttg cggcaccagt atgtgaggat catttctgca
     7261 tgctggcgaa gattgttttt tgtttataaa taatcataat atatttgact atcaaacaaa
     7321 tctaatttta agaaatgcga aactcagtaa caggaggtat tctgtctgcc ttatttgtct
     7381 tactgacaac atcgtcggga catgcccaga acgagcggct gtcattcggt aagtccgtat
     7441 cgttagacaa agccattttc agtacaaagg agaaagagca gccgttgatc tggataaatg
     7501 taaatacgga tcgggataca tggcatgttg agaaggatat cctggtctgc tccggtaaac
     7561 ccata
//
LOCUS       DAOOYG010000134         7456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_11196_length_7456_cov_5.677341, whole genome shotgun sequence.
ACCESSION   DAOOYG010000134 DAOOYG010000000
VERSION     DAOOYG010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11196_length_7456_cov_5.677341"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PK288_09865"
     CDS             <1..432
                     /locus_tag="PK288_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19272.1"
                     /translation="ILPRVHYDYGIVAVDAGVDVSVNARTFMKFGQGTTFGMGVLAEG
                     HAYLGGSCPATCTSANADAKLQFGVSGDYNTKTNVFNIDGCASVNLVISASQCLPVLV
                     DCGPCVSVDLADFTIGAKVHLDNVKGFSMGITTSSCDQQCR"
     gene            459..2471
                     /locus_tag="PK288_09870"
     CDS             459..2471
                     /locus_tag="PK288_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19273.1"
                     /translation="MKISGRHQGYLPGISLLLVLCLLLNSPAWGQREVKLAVPAPRGI
                     VVFAGMELADGKRLGSYSIERSYDGRKWENVTQMHSPAGWDEFYAAIRLWESDFGFQG
                     LPGNDVLRTGWEKCARSGVIDSMGYWASATAIRLAAGLAFYDKDVTKDKQVRYRVKSL
                     KNGKTVSESLSLPVQYPFIPEYDAVTLSEKNVDQDICYLKWQSKGANPAPYFEVRYYE
                     GKELHQAKGTTATYRIDDVTYFIFQDSLRSFRSDRQYFLNPLDKWGNRGEATDIVLVS
                     GKSPGTTFFRNVSATSDNTGYGIVVTWQSQGTDDLRGVRIYRSDSYDGKPYDLVANLP
                     PSDSSYTDREVKPDQTTYYYLESVSDVGDPPRRSGIVFGSALDRLQPVFPSVSRGEEV
                     PGGVAIKVTSGEPNLAGVRIYRSDGITSSMYPITGILELTDNEVVYIDTSSYLSGDQS
                     YLYAATTVNTSSVESIFSDTLIIHPSIRTIPPSPNQLTAAEEDGGVSLVWEDVKVRNR
                     ATRGYHVYRRELPGGQFTSLLPGESAVDIPFYTDKTVLPGKSYEYAVQTVDDLGGVSE
                     SMAVITLTVKELFLPVPPGVWLGQAAGKVIVQWAESTGDIGLKTNLYRYRRGEKPSLL
                     KSFSHGERRYEDGIVKKGELWFYFTTFTNGEGREGARSPEAGIRVE"
     gene            2807..3493
                     /locus_tag="PK288_09875"
     CDS             2807..3493
                     /locus_tag="PK288_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19274.1"
                     /translation="MKKILVFTVLICFMAAAHGQETFFPTKEGTVLVYKTFDKKSKET
                     GSIRYTIEKVSISGNDMDISYLVESSDSRGASLYKDKITIQQRDGRLYMDMSNMVNKA
                     AFQQDGEIPAELEITGNKMEIPVGIQPGQTLPDASVVMAMKMGFVSMKMAADITNRKV
                     DAIEDVTVPAGTFKCYKLSGDVSATVMGMNVKTSNLDWYAKGIGLIRSESYDKSNKLQ
                     SFIQLTELRK"
     gene            complement(3497..6454)
                     /locus_tag="PK288_09880"
     CDS             complement(3497..6454)
                     /locus_tag="PK288_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPM19275.1"
                     /translation="MKKTIIRSLFAISLMFCTLSSPAQTKEPPFLRYLNHPWVDSVMK
                     GLTLEQRIAQCIWVAGYSNRDVAHEVEITETIKKYGIGGIVFFQGTPEKQAALTNYYQ
                     GVSAVPLFIAMDAEWGLGMRLNNVDKFPYQMTLGATGNDLLIYRMGEAVARQMKRMGV
                     HVNLAPVADINNNPLNPVINYRSFGEEKVNVTSKSMMYMKGMQDNGVLACIKHFPGHG
                     DTKTDSHLDLPVIPHSMTRLESIELFPFLSLINEGAGCIMTAHLNLPALDPRPGLPSS
                     LSRPIVTDLLKTRMNFRGLVITDAMNMKGVTNYFSNGLADAKALAAGNDVIEFVTDVQ
                     AAISETMKLIEKKEMTRGDIDNKCRKILAAKYWAGLGKFREIPAENLTHDLSDPSAKA
                     LIRELYASSLTVLNNEEQLIPVKNLDKIRVATLAINRNKTSAFQARLMNYHPADAFTA
                     DPSNHEQSEELISRLSEYDLVIAGIYNTDQRPGNNFGISRDLNPFLEKLVNGAKTIFV
                     YFGNPYALDRLEALQKSAGLILAYQENSIAEDLSAQLIYGGISGKGHLPVTINAKWPA
                     GFGLATPGNLRTAYGLPENAGVSSELLTARIDSIATSGLTIRAYPGCEVMVARKGIVI
                     FHKTYGYHTYENRTPVMEDNLFDLASVTKISSTLAGLMLLDTRGQFSPEKTLGEYLPF
                     FRGSNKENLTMKELLAHQAGLTPFILFWKETMKDDSTYKRSIIRHEYSSRFPLKVADN
                     LYINENFRHKMFAEIKKSPLGEKKYVYSDLTFIISPQIIEHLTGEKWNEFVEKNIYHK
                     LGAYDIVFNPYLKYPPERIIPTEYDSVFRKQQLLGTVHDEAASLQGGISGHAGLFATA
                     GDLMKLMELFRRMGRYGGEQIIAEDVLREYTRVQFPENDNRRGLGFDKPFLNNAELPQ
                     EETYPTRGASPESFGHSGYTGPFVWIDPVHEISYVFLANRVYPTRNNNKISENNIRTN
                     ILQAVYDSITDKP"
     gene            6647..>7456
                     /locus_tag="PK288_09885"
     CDS             6647..>7456
                     /locus_tag="PK288_09885"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HPM19276.1"
                     /translation="MAKAGRLISLDIFRGITIAFMIIVNNPGSWNYVYPPLKHAEWHG
                     CTPTDLVYPFFLFIVGVSIWFSMKKYGHELNGPSVFRIFRRTLAIFALGLFLAIFPYF
                     SRDYSTLRIMGVLQRIALAYGFAALICLSVNRKYLWIVTAVILLLYWGVLYFFGSSDP
                     YSLEGNFAIKVDEAVLGKSHMYRGYGIPFEPEGLLSTIPSICTVIIGFYAGELAGKGQ
                     GGTKTVLKLLLIGVSLTGLGLLWNEFFPFNKPLWTSSYVHYSSGIAMACLAV"
BASE COUNT         1836 a         1687 c         1867 g         2066 t
ORIGIN      
        1 atactgccga gagtacatta tgattacggt atcgttgccg ttgatgccgg cgtcgacgtg
       61 agtgtcaatg cccgtacatt catgaaattc gggcagggaa caactttcgg gatgggcgtc
      121 ctggcggagg gtcatgccta cctcgggggc tcatgcccgg cgacatgcac atcggccaat
      181 gctgatgcta agcttcagtt tggagtcagc ggtgattaca acaccaaaac aaatgtcttc
      241 aacatagatg gctgtgccag cgttaacctg gtaatatcgg ccagccagtg ccttccggtt
      301 ctggtcgact gcggaccatg cgtgtcggtt gatcttgccg attttaccat tggcgccaaa
      361 gtacatcttg acaatgtgaa gggtttctca atgggaatca caaccagcag ctgtgatcag
      421 caatgcagat aattatggac atgctaacca ttaacaaaat gaaaatatca ggaagacatc
      481 agggatattt gccagggatc agcctcctgc ttgttttgtg cttattgctg aattcacctg
      541 cctggggaca gcgtgaggtt aaactggcag tgccggctcc ccggggtatc gtggtattcg
      601 ccggtatgga gcttgctgat ggtaagcgtc ttgggtccta cagcattgaa cgctcgtatg
      661 atggccgaaa atgggagaac gttacccaga tgcattcacc cgcaggttgg gatgagttct
      721 atgctgccat acggctgtgg gaatccgatt ttggcttcca gggcctccca ggcaatgacg
      781 tgcttagaac gggatgggaa aaatgtgcca ggtcgggtgt aattgacagc atgggatact
      841 gggcttcagc cactgcaatc aggctggcag ccggattggc attctatgat aaggatgtga
      901 caaaagacaa acaggtcagg tacagggtta aatcgctgaa gaacggaaag actgtttcgg
      961 aaagtctgtc gctgcctgtt caatatccct ttatcccgga atatgatgct gtcacgctga
     1021 gtgaaaaaaa cgttgatcag gatatctgtt acctgaaatg gcaatcaaag ggggccaatc
     1081 ctgctcccta ttttgaggta agatactatg aaggaaaaga acttcatcag gccaaaggta
     1141 ctaccgcgac ctacaggata gatgatgtga cttactttat ttttcaggat tcgttgcgga
     1201 gcttccgttc agaccggcaa tatttcctga acccgcttga caagtggggg aacaggggtg
     1261 aagccactga tatcgtgttg gtttcgggca aatcgccggg tacgacattc ttccgcaacg
     1321 tttcagcaac gtccgacaac accgggtatg gcatagttgt cacctggcaa tcgcagggta
     1381 ctgatgattt acggggtgtc aggatttaca ggagcgattc atatgacggt aagccatatg
     1441 atctggttgc gaaccttcca ccttccgaca gcagctacac tgaccgggaa gtaaaacctg
     1501 atcagaccac ttactattat ctcgagtctg taagtgatgt cggtgatcca ccccggagga
     1561 gtggtatcgt tttcggttct gccctagaca ggcttcaacc ggtatttcct tctgtcagtc
     1621 gcggtgaaga ggtacctggc ggcgtggcta taaaggtgac ctcgggcgag cctaatctgg
     1681 caggtgtccg tatttatcgc agtgacggca taacttcttc gatgtatccc atcaccggca
     1741 tcttggaact gacggataat gaggtggttt atattgatac cagcagctac ctcagcggtg
     1801 accaaagcta cctctatgct gctacaacag taaataccag ttccgtagaa agtatttttt
     1861 cagacactct catcattcat ccttccatca gaactattcc accttcacct aaccagctga
     1921 cagcagcgga ggaggatggc ggagtctcat tggtctggga ggatgtaaaa gtcaggaaca
     1981 gggccacacg gggctatcat gtttaccggc gcgagctgcc cggtggtcag tttacaagcc
     2041 tgttgccggg cgagagtgca gtggacatac cattctacac tgacaagacc gtcttacccg
     2101 ggaaaagcta cgagtatgct gttcagacag ttgatgatct gggtggcgtg agcgaatcca
     2161 tggctgttat cacgctaaca gtaaaggagt tgtttcttcc tgtccctccc ggtgtatggc
     2221 tcggccaggc cgctggaaag gttatcgtgc aatgggcgga gtcaaccggt gacattggcc
     2281 ttaagacaaa cctctaccgt taccggcggg gtgaaaaacc atcgctcctg aagagcttca
     2341 gtcatggtga gcgaagatac gaggacggga tcgtgaagaa aggggagtta tggttctatt
     2401 tcaccacttt caccaacgga gagggcaggg aaggcgcccg ttcgccggaa gcgggtatca
     2461 gggtggaatg acactgcagt cagtcagaat caaaacaacc cgacagcggc aatatcaata
     2521 aatctgggac ttgtcgagcc gcagcctgct atacggaaga accgggaagg aggggagtac
     2581 gggattttaa tgtgtgcatc cgtatcagca gcacatggtt tatcatatat aaattattga
     2641 gtggccggtg ttccgggaat acaaaaaaac tgttattttg tagtgcattt tcaccagtta
     2701 tattgtctgc gcgggtagcc gggcaggttg tatcatttcg cagcacgatt tttgctcaaa
     2761 ccttattcat tattagtatt tcgtaaatct ttaattcaat ccgtttatga aaaaaattct
     2821 ggtattcaca gtgcttattt gttttatggc tgccgcacac ggccaggaaa ccttcttccc
     2881 cacaaaagaa ggaacagttt tggtttataa gacgtttgat aaaaaatcaa aggaaacagg
     2941 ttccatcagg tataccattg agaaagtgag catcagcggc aatgatatgg atatttccta
     3001 tctggttgaa tcttctgact cccgtggagc ctctctttac aaggataaaa tcacaattca
     3061 gcagagagac ggcaggttgt acatggatat gagcaatatg gtcaacaagg ctgctttcca
     3121 gcaggacgga gaaatcccag cagaacttga gataacaggg aataaaatgg aaattccggt
     3181 cggtattcag cccgggcaga cgcttcccga tgcttcagtg gtcatggcaa tgaagatggg
     3241 atttgtcagc atgaagatgg ccgccgacat aacaaacagg aaagtggatg caattgagga
     3301 tgttaccgta cctgccggta cttttaaatg ttataagctt tcaggtgatg tcagtgccac
     3361 ggttatgggg atgaatgtta agaccagtaa ccttgactgg tatgcgaagg gcatagggct
     3421 gatcaggtca gaatcctatg acaagagtaa caagctgcag tccttcatcc aattgacaga
     3481 gctaaggaaa tgaatatcag ggtttgtctg tgatagaatc ataaactgcc tgcaggatat
     3541 tggtcctgat attgttttca gatattttgt tgttgtttcg tgtcggatag acccggttgg
     3601 caagaaaaac ataacttatt tcatgaaccg ggtctatcca gacaaaggga ccggtatatc
     3661 ccgagtgtcc gaagctttcg ggtgatgctc ccctggtggg ataagtttct tcctgcggaa
     3721 gttctgcatt attcaggaaa ggtttgtcaa atcccagtcc tctcctgtta tcgttttccg
     3781 ggaattgcac cctcgtatac tccctgagaa catcttctgc tattatctgt tctcctccgt
     3841 accgtcccat tctcctgaat agttccatca gcttcatcag gtcgcccgcc gtggcaaata
     3901 acccggcatg ccccgatatc ccgccctgaa gcgatgctgc ttcatcatga acagttccca
     3961 gcagctgctg tttcctgaag acagagtcat attcagttgg aataatcctc tcaggcggat
     4021 atttcagata cggattgaac acgatatcat aggccccaag cttatggtaa atattctttt
     4081 caacaaactc attccatttt tccccggtaa gatgttcaat gatctgcgga gaaataataa
     4141 atgtcaggtc agagtataca tacttttttt ctcccagcgg gctctttttt atttctgcga
     4201 acattttgtg cctgaaattt tcatttatat aaaggttatc ggcaacctta agcgggaatc
     4261 ttgaagaata ctcatgcctt attatgctcc ttttatatgt tgaatcgtct ttcatggtct
     4321 ctttccagaa aagaataaaa ggggtcagtc ctgcctgatg tgccaggagc tccttcatgg
     4381 tcaggttttc cttgttcgat cccctgaaaa acggcagata ttcccccagg gttttttcgg
     4441 gggagaactg gccccgggta tcaagtaaca tcagtccggc aagtgttgat gatattttcg
     4501 ttacagaagc caggtcgaaa agattatcct ccattacggg tgtcctgttc tcatatgtat
     4561 gatacccata agttttgtgg aatatgacta ttccctttct ggcgaccatc acctcgcagc
     4621 cggggtaggc cctgattgtt aagcctgaag tggcgattga atcgatcctg gctgtcagta
     4681 attcagatga tacgcctgcg ttttcaggga ggccgtatgc tgtgcgcagg ttaccgggtg
     4741 tcgccagccc aaagcctgcg ggccattttg cgtttattgt gaccggcaga tgtccctttc
     4801 cggaaatacc cccgtagata agctgggccg aaaggtcttc cgcaatgctg ttctcctggt
     4861 aagcgagtat taatcccgcc gatttttgaa gggcttccag tctgtcaagt gcatatgggt
     4921 tcccgaagta tacaaagatg gtttttgcgc cgttgacaag cttttcgagg aaaggattga
     4981 gatcgcggct gattccgaaa ttattgccgg gtctttgatc cgtattgtat attcctgcta
     5041 taacaagatc atattctgac aggcggctaa tcagctcctc tgactgttca tgattggaag
     5101 ggtctgccgt aaatgcatcg gctggatgat aattcatgag tcttgcctga aaggctgatg
     5161 ttttattcct gtttattgcc agtgttgcaa ccctgatttt gtcgaggttt ttcaccggaa
     5221 tcagttgttc ttcgttattc agcactgtga gggaactggc ataaagctcc ctgataaggg
     5281 ctttggcgga aggatctgac agatcgtgtg tgaggttttc tgccggtatc tcccggaatt
     5341 tgccaaggcc ggcccaatac ttggctgcaa ggatcttcct gcatttattg tcgatatctc
     5401 cccttgtcat ttccttcttt tcaataagtt tcatggtttc acttattgca gcctgaacat
     5461 cggttacgaa ttcgatcacg tcgtttccgg ctgcaagtgc ttttgcatct gccagtccgt
     5521 tgctgaagta gtttgtaacc cccttcatgt tcatcgcatc ggtaatgacc aggcccctga
     5581 aattcatccg tgtttttaaa aggtcggtta caatggggcg cgacagtgat gaaggcaatc
     5641 cgggtcttgg gtcgagtgcg ggaaggttaa gatgtgcggt catgatacag cctgcgcctt
     5701 cgttgatgag gctcaggaaa gggaacagct cgatgctctc gagccttgtc atggaatgtg
     5761 gtatgaccgg caggtcgaga tgagagtcgg tttttgtatc gccgtgaccc ggaaagtgct
     5821 tgatgcatgc cagcaccccg ttgtcctgca ttcctttcat atacatcatc gacttcgaag
     5881 tcacattcac tttttcctcg ccaaacgacc ggtagtttat caccgggttg agcggattgt
     5941 tatttatgtc ggccacggga gccaggttta catgcacacc cattctcttc atctgcctgg
     6001 caacggcttc acccatcctg taaataagca gatcatttcc agtagcgccc agagtcatct
     6061 gatagggaaa tttatcaaca ttgttgagcc gcattcccag accccattca gcatccattg
     6121 caataaaaag aggaacagcg gaaacaccct ggtaatagtt cgtcagtgcg gcctgctttt
     6181 caggagtgcc ctggaaaaat acaataccgc caatcccgta ttttttgata gtttcggtta
     6241 tttcaacttc atgtgccaca tccctgtttg aatatcctgc tacccagatg cattgcgcta
     6301 tccgttgttc cagggtcagt cctttcataa cggagtcgac ccaggggtga ttcagatacc
     6361 tcagaaaagg aggttccttc gtttgcgccg gtgacgaaag ggtacagaac attaagctga
     6421 ttgcgaacag agatctgatt attgtctttt tcatggagtt tgaaacttat actgatcgtg
     6481 gataattatt ataaatgaac tcaaatgtac aaatttttat cacccctgcc acaggacaag
     6541 ggactgattg ccccgggcgg gaaaaacgta aataaattca gacaatccat tgcatgagcg
     6601 gtcaaattat taaattgcag gggttatttg cagttgtatt tcagaaatgg caaaggcagg
     6661 aagactgata tccctggata tattcagggg aattaccata gctttcatga tcatagtaaa
     6721 caatccgggc agctggaatt atgtttatcc tccgcttaag catgcggagt ggcacggatg
     6781 cactccaaca gatcttgtat accctttttt cctttttatt gtgggtgttt ccatctggtt
     6841 ttcgatgaaa aaatatggtc atgaactgaa cggaccttcc gttttcagga tattcagacg
     6901 cactcttgcc atttttgcac tgggcctgtt ccttgcaata ttcccctatt tctccaggga
     6961 ttactctaca ctcaggataa tgggagtgct ccagaggatt gcccttgcct atggattcgc
     7021 tgccctgatc tgcctgtccg tgaacaggaa atatctctgg atcgttactg ctgtcatact
     7081 gttgctttac tggggggtcc tttatttctt cggaagttcc gacccgtaca gtcttgaagg
     7141 caattttgcg atcaaggtcg atgaggctgt ccttggaaag agccatatgt acaggggcta
     7201 cgggatacct ttcgaacctg agggcctgtt gagcaccatt ccttcgatct gtacagtgat
     7261 aattgggttt tatgccggtg agcttgcagg gaaaggacag gggggtacca aaacggtgtt
     7321 gaaactgctg cttatcggtg tatcccttac cggactgggg ctgctgtgga acgaattctt
     7381 tcccttcaac aagccgcttt ggaccagctc atatgttcac tactcttccg gtatcgcaat
     7441 ggcctgcctg gctgtt
//
LOCUS       DAOOYG010000135         7345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_11428_length_7345_cov_7.062826, whole genome shotgun sequence.
ACCESSION   DAOOYG010000135 DAOOYG010000000
VERSION     DAOOYG010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11428_length_7345_cov_7.062826"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..465
                     /gene="rpsK"
                     /locus_tag="PK288_09890"
     CDS             76..465
                     /gene="rpsK"
                     /locus_tag="PK288_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPM19277.1"
                     /translation="MAKKTGAQKKRIVKVEPNGQAHIHSSFNNIIVTLTNNSGQVISW
                     ASAGRMGFKGSKKNTPYAAQMASEECSKVAYDLGLRKVKVFVKGPGAGRESAIRSINN
                     AGIEVTEIVDVTPMPHNGCRAPGRRRV"
     gene            509..1117
                     /gene="rpsD"
                     /locus_tag="PK288_09895"
     CDS             509..1117
                     /gene="rpsD"
                     /locus_tag="PK288_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPM19278.1"
                     /translation="MARYIGPKSRIARKFGEPVYGPDKYLDRKNFPPGQHGINKKRKK
                     TSEYGVQLREKQKVKYTYGILERQFRNTFEKASASKGVTGEELLKLLESRLDNVVYRF
                     GIALTRASARQLVSHRHITVNGNVVNIPSYQLSPGDIVGVREKSKSLERITESLATRK
                     YSRLPWLEWDDAQMAGKFMTVPERSDIPEDIKEQLIVELYSK"
     gene            1136..2128
                     /locus_tag="PK288_09900"
     CDS             1136..2128
                     /locus_tag="PK288_09900"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPM19279.1"
                     /translation="MAILSFQKPDKVIMLEANNRLGKFEFRPLEPGYGITVGNALRRI
                     LLSSLEGYAITTVKIDGIDHEFSTITGVIEDVTEIILNLKQIRFKRIVEDVDHEKVTV
                     KFSGQDVFRAGNLNSVLSSFEVLNPDLVIFRMEPDVKIQMELTVNKGRGYVPAEENQP
                     ADSEFGLIALDSIFTPIKNVKYTVENYRVEQKTDYEKLLLEIETDGSVHPKEALKEAA
                     KILIYHFMLFSDEKITLDSDDKLANEEFDEEILHMRQLLKTKLIDLDLSVRALNCLRA
                     AEVETLGDLVKFNKNDLLKFRNFGKKSLTELDERLESMNLSFGMDVAKYKLDKD"
     gene            2155..2718
                     /gene="rplQ"
                     /locus_tag="PK288_09905"
     CDS             2155..2718
                     /gene="rplQ"
                     /locus_tag="PK288_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPM19280.1"
                     /translation="MRHNKVINHLGRTSSHRKAMMANMATSLIMHKRITTTLAKAKAL
                     RSYVEPLITKSKDDSTHSRRVVFSYLQDKTAVSELFREISPKIADRPGGYTRILKTGS
                     RLGDSAEMCIIELVDYNEGMLGIETTKAKPGRRRRSAKKTRTETDASKTAKPASAKAK
                     QVPETPAVDDKSPEPDATDVAESEDKE"
     gene            2940..4217
                     /gene="eno"
                     /locus_tag="PK288_09910"
     CDS             2940..4217
                     /gene="eno"
                     /locus_tag="PK288_09910"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPM19281.1"
                     /translation="MGQIVNVHAREILDSRGNPTIEVDVLTASGFQGRAAVPSGASTG
                     ENEALELRDGDESRYLGKGVLKAVHNVNNVIAEEVMGMDVTEQAAIDRKMLELDGTKT
                     KSNLGANAILGVSLAVAKAAAAYHGLPLYRYIGGSNAITLPVPMMNIINGGSHSDAPI
                     AFQEFMIRPVGATSFHEGLRMGAEVFHSLKKVLKKRGLSTAVGDEGGFAPNLDGPEDA
                     LETIVNAITAAGYKPGSDITIALDAAASEFYKDGIYDYSRFQGQNAPRKSSAEQVDYF
                     EKLTGMFPIDSIEDGMAESDWDGWKLMTQRLGHKIQIVGDDVFVTNVEYLKKGIELGC
                     ANSILVKLNQIGSLTETLNAIDMAHRAGYTAVISHRSGETEDSTIADVAVAVNAGQIK
                     TGSCSRTDRICKYNQLLRIEESLGNLAVYGYKK"
     gene            4593..4724
                     /locus_tag="PK288_09915"
     CDS             4593..4724
                     /locus_tag="PK288_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19282.1"
                     /translation="MFSRSEKIGNVLSTIGSQGFRKKRLRSRFPETGAIPSGIDPRA"
     gene            4986..6884
                     /locus_tag="PK288_09920"
     CDS             4986..6884
                     /locus_tag="PK288_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HPM19283.1"
                     /translation="MAYSGLSSYIRKLEEEGELHRIKSFVNPVLEITDIADRVFKAGG
                     KALLFENTGTRFPLLINAFGSGKLISLALSQTDISSAAAEIEKIPGLLTRKHASLLNK
                     ISALPKLLELSKFLPATSRKKGKCQQIVINDPDLSIFPVLKCWPHDGGRFFTLPMVHT
                     SDPETGATNVGMYRIQILNASTAAMHWHRHKTGAKHFEAWKKKKCRMPVTVTLGGDPV
                     YAYSATAPLPENINEYILAGFLRKKKVRLVKCLTNDLYIPEDADIVIEGYVDPDEGLV
                     VEGPFGDHTGFYSLEDYYPRFHVKCITYSENAVYPATIVGIPPQEDAFLAEATEKIFL
                     APVKLALQPEIEDFHMPDAGTAHNLLIVKISKSYPGQGLKVVSSLMGTGQMMFAKYVF
                     VVSGTVNIRDYSELAGHIMANTDFATDIFFTKGPLDVLDHASDSFSFGGKAGIDSTEK
                     FPEERSGHASEAGSLPDDAASDPLTSLPDTGLIRSINADLYNAGLGILILTVNPSEEP
                     DCIHEVARLFGNHKHSSSFRLILAVDHTVDVTDLFMVAWQVLANSDPIRDHIWISGSV
                     LLIDGTIKHYRQNGFPRPWPNIVCSDMETIKAVDEKWQDLGFDGFIPSPSLKYIPLVR
                     KGRARIED"
     gene            7019..>7345
                     /locus_tag="PK288_09925"
     CDS             7019..>7345
                     /locus_tag="PK288_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19284.1"
                     /translation="MKQNFGILFLAAFICFLAGGSVANGQPPEGMVKFTPDFRFVDGV
                     FLNFDQVKANDPIPKAKLLTSTDYNDKDFFKNLFSGDKIYYYDGMGIRQEVDKKTVWG
                     YSRNGIL"
BASE COUNT         2195 a         1637 c         1740 g         1773 t
ORIGIN      
        1 taatgccaga acccgcaagg gaagaaagaa aaccgttgca aataagaaaa aagctactaa
       61 ataattaatt ggaacatggc aaaaaaaacc ggagcgcaga agaaaagaat tgttaaggtg
      121 gaacctaacg gtcaggctca tattcattcg tcgttcaata atatcattgt tacactcaca
      181 aacaattccg gtcaggttat ttcatgggcc tcggcaggaa ggatgggctt caagggatcg
      241 aaaaagaaca ctccatatgc agcccagatg gcatcggaag aatgttcaaa ggtggcatac
      301 gatctgggac tgagaaaggt aaaggtcttt gtcaagggcc cgggcgctgg tcgtgagtcg
      361 gctatcaggt caatcaacaa tgcaggaata gaggtcactg aaatagtcga cgtgacacca
      421 atgccgcata acggatgccg tgcaccaggc agaagacggg tgtgatttca aacttcaaac
      481 tttttccagg aatttaaaat aagcgaacat ggcaagatat attggcccga aatcgagaat
      541 tgcaagaaaa ttcggggaac ctgtttacgg acccgataaa tacctcgaca gaaaaaattt
      601 cccgccgggg cagcacggga tcaacaagaa aagaaagaaa acatcggaat atggtgttca
      661 gctgagagaa aagcaaaagg tcaagtacac ttacggaata cttgaacgtc agttccggaa
      721 tacctttgaa aaagcttcgg ccagcaaggg tgtaacgggt gaggagcttc tgaaactcct
      781 tgaatcacga cttgataatg tggtataccg ttttggaatt gcacttacaa gggcttctgc
      841 aaggcagctt gtttcacacc gtcatatcac tgtaaatggc aacgtggtca acataccatc
      901 atatcagctt agccccggtg atattgttgg tgtccgcgag aaatcaaaat ccctcgaaag
      961 gataacagaa tccctcgcca caagaaaata ctcaaggctc ccatggctgg aatgggacga
     1021 tgcacaaatg gcaggcaagt tcatgactgt gccggaacgc tctgatattc ctgaagatat
     1081 taaggaacag ctaatcgttg aattgtattc caagtaataa tataagctaa attctatggc
     1141 cattctatca ttccaaaagc ctgacaaggt aataatgctt gaagccaata accgattggg
     1201 gaaatttgag tttcgacccc tcgagcccgg ctatggcatt accgttggaa atgccctgag
     1261 gagaatcctt ctgtcttccc tggagggata tgcaattacc acggtgaaaa tcgacgggat
     1321 cgatcacgaa ttctccacaa tcacaggagt tattgaggat gtaaccgaga tcatccttaa
     1381 cctcaagcag atccgtttta aaagaattgt cgaagacgtt gatcacgaaa aggtgacggt
     1441 caaattcagc ggccaggatg tgttcagggc cggcaacctg aacagcgtgc tgagcagctt
     1501 tgaagttctc aatcccgatc tggtaatctt cagaatggaa ccagatgtaa aaatccagat
     1561 ggaactgacc gttaacaagg gaagaggata tgtaccggct gaggaaaacc agccagccga
     1621 cagtgaattc gggctgattg cactcgactc gatattcaca cctataaaaa atgtaaaata
     1681 tacagtcgag aattaccggg tagagcaaaa aaccgactac gagaaactcc ttcttgaaat
     1741 agaaaccgac ggttccgtcc acccaaagga agcccttaag gaagccgcaa aaatactgat
     1801 ttatcacttt atgctgtttt ccgatgaaaa gattaccctc gattcggacg ataagcttgc
     1861 aaatgaggaa tttgatgaag aaatactgca catgcgtcag ctgcttaaga ctaagctgat
     1921 tgacctcgac ctgtcagtca gggcgctgaa ctgccttagg gcagctgagg ttgaaaccct
     1981 tggcgacctg gttaaattca acaagaatga ccttctcaaa ttcaggaatt tcggaaagaa
     2041 atcccttacc gagctggatg aacgccttga gtcgatgaac ctgtcgtttg gaatggatgt
     2101 ggcaaagtac aagcttgata aagactaatt taaaaccgga taaggaataa acaaatgcga
     2161 cataataaag taattaatca tctcggaaga acaagctcac acagaaaggc aatgatggcc
     2221 aatatggcaa cgtcgctgat tatgcacaag agaattacca caacgcttgc caaggcaaaa
     2281 gccctcaggt cctatgttga gcctctgatc acaaagtcaa aagatgattc cacacattcc
     2341 aggcgggttg tattcagtta cctgcaggat aaaactgctg tctccgaact tttcagggag
     2401 atttccccta aaattgcaga caggccgggc ggatatacaa gaatactgaa aacaggaagc
     2461 aggttgggcg atagtgctga aatgtgcatt attgagctcg ttgattacaa tgagggtatg
     2521 cttgggatag aaacaaccaa agccaaaccc ggaagacgca gaaggtccgc aaagaagacc
     2581 cgtaccgaaa cggatgcttc aaaaacagca aagcctgctt ctgcaaaggc aaaacaggtt
     2641 ccggaaactc ctgcagttga tgacaaatca cctgaacctg atgccaccga cgttgctgaa
     2701 tctgaagaca aggaataatt agcaatgcaa tgtttccctc atgttaaaat acattaccgg
     2761 gtacgggaac acatcaatga aggacactaa tgagacaagc attcttacct gcggctctgc
     2821 catatggctg acctgagatg ctgaacccgg acctgtaatt gcccgatttt taaaacaccc
     2881 gtaaaggcat tgaaccaagt tcgaatactt accattgtca aataaaaaaa taaattccta
     2941 tgggacagat agttaatgta catgcccgtg aaattctcga ttcccgcggc aatcctacaa
     3001 ttgaagttga tgttttaacg gccagcggtt tccagggacg ggctgcagtg ccttcaggtg
     3061 cctctacggg cgaaaatgag gcgcttgaac tccgcgacgg ggatgaatcc cgttatctcg
     3121 gaaagggagt cttgaaggct gtccataacg taaacaacgt aatcgctgaa gaagtcatgg
     3181 gtatggatgt caccgagcag gctgccattg acaggaaaat gctggagctc gatggtacta
     3241 aaaccaagag caacctgggt gcgaatgcca tcctgggtgt atcccttgct gtcgccaaag
     3301 ccgccgccgc ttaccacggg cttccccttt accgctacat aggaggttca aatgcaataa
     3361 cccttcctgt gccaatgatg aatataataa acggaggatc ccattccgac gcacccattg
     3421 ctttccagga gtttatgatc aggccggttg gagcaaccag tttccatgag ggcctcagaa
     3481 tgggtgccga agtcttccat tctctcaaga aagtgctgaa aaaacgcgga ttaagcacgg
     3541 ctgtcggtga tgagggaggc ttcgccccga atctcgacgg gccggaggat gccctcgaaa
     3601 ctattgttaa tgcaataaca gcagccggat ataaacccgg ttccgatatc accatagccc
     3661 ttgatgccgc agcgtccgag ttttataaag acgggatcta tgattattca aggttccagg
     3721 ggcagaatgc accccggaaa tcatctgccg aacaggttga ttactttgag aagctgacag
     3781 gtatgtttcc aattgattct attgaggatg gaatggctga aagtgattgg gacggttgga
     3841 aactgatgac tcaaaggctc ggacataaaa tccagattgt gggcgatgat gtttttgtca
     3901 ccaatgttga atatctgaaa aaaggtattg aactcggctg tgccaactcc atacttgtta
     3961 aactgaacca gatcggttca ctcacggaaa cactcaatgc catcgacatg gcccacaggg
     4021 caggttacac tgctgtgatc tcacaccgtt ccggcgaaac tgaagattca accattgctg
     4081 acgtggctgt tgcagtgaat gcagggcaga ttaaaacagg ctcctgcagc aggactgaca
     4141 ggatctgcaa atacaaccag ctgctcagga ttgaggagtc acttggtaac cttgctgttt
     4201 acggatataa aaaataattc attcagaaat tgaataatta ctataccagg cattttacgg
     4261 ggaaacacag atatccgctt tgtgtaaacc cgccagacta ttgcaagccc atgtgtgaat
     4321 acatacatgg gcttcatttt tcggactttt ccgacgttat taacaattgt tgataacgtg
     4381 tgtttatatc ccgtgtaaaa taatgaaaag ctgcctggtc gttgtatcat tttcttatat
     4441 tacatttggt ctatcagatg agagataaaa ggttgctcaa atgatgcgga accggaaaag
     4501 gtttcttaag tggaacaagt tccggttctc tcggaccttc gggaccgcgc accaggagag
     4561 ccgaagtctg gacgaatgaa ccccggttca ggatgttcag cagatcggag aaaatcggaa
     4621 acgttctttc aacgattggg tcacagggct tcaggaaaaa gagacttcgg tcacgatttc
     4681 ccgaaactgg tgcgattcct tcgggaattg atcccagggc ctgaatgaca cttcgtcccg
     4741 gaagtcccgc tccggcggga aaaaaggaca gccggaaggt tctgccgtaa aaggcagatc
     4801 cggaaagccc agggcagtaa agcctgaccc agcgtcagga aataccgccg accaaaataa
     4861 cagtaatctg acgtcataca cggcttcggc tgtaaatgat ggatgggaac taatccaagg
     4921 aatagttccc atcactgttt tatatacccc catattcaat atctttatgc ctttaaatat
     4981 tcataatggc atactccgga ctatcatcat atatcagaaa gcttgaggaa gaaggcgaac
     5041 tccacagaat aaaatcattc gttaatcctg tccttgaaat aaccgatatt gccgacagag
     5101 tcttcaaggc aggcggaaag gcgcttttgt tcgaaaatac gggaaccagg tttccactgc
     5161 ttattaatgc cttcggttcc ggcaaactca tttcacttgc attaagtcag acggacattt
     5221 catctgccgc cgctgaaatt gaaaaaatcc ccggtctgtt aacccggaag catgcttcac
     5281 tcctgaataa gatctcagcc ctgcccaaac tcctggaact gtcaaaattc ctgcctgcga
     5341 caagccggaa aaaggggaaa tgccagcaga tcgtcatcaa tgacccggat ctttcaatat
     5401 tcccggtact gaaatgctgg cctcacgacg gggggcgctt ttttaccctc cccatggtcc
     5461 atacttctga tcctgagacc ggcgcaacta atgttggaat gtacagaata cagatcctca
     5521 atgccagtac ggcggcaatg cactggcaca ggcataaaac cggtgccaaa cattttgaag
     5581 cctggaaaaa gaaaaaatgc agaatgcctg tgactgtaac actgggaggc gatccggttt
     5641 atgcttattc tgccactgct ccccttcctg aaaatatcaa tgagtacatt cttgccggat
     5701 tcctcagaaa gaaaaaggtc aggctggtta aatgccttac caacgacctg tacattcctg
     5761 aagatgcaga tattgtcatt gaaggatacg ttgatccgga tgaagggctg gtcgtggaag
     5821 gcccgttcgg tgaccatacg gggttttatt cccttgaaga ttactacccg aggttccatg
     5881 taaaatgcat aacttactct gaaaatgccg tatatccggc aacaatagtg ggaatacctc
     5941 cccaggaaga tgccttcctt gcagaagcaa ctgaaaaaat attccttgcc ccggttaaac
     6001 tcgccctgca gcccgaaata gaggatttcc acatgccaga tgcaggaact gcccataatc
     6061 tcctgattgt caaaattagc aaaagttacc cgggccaggg attgaaagtg gtcagttcac
     6121 tgatgggaac aggacagatg atgtttgcaa aatacgtgtt tgtggtaagc ggaaccgtca
     6181 acatccgcga ttacagtgaa ctggcagggc atataatggc aaataccgat tttgcaactg
     6241 acattttttt tacgaagggc cccctcgatg tccttgacca tgcatccgac agcttctctt
     6301 tcggggggaa agccgggata gattcaacgg aaaaatttcc cgaggaaagg tcaggtcatg
     6361 ccagtgaagc cggttcactg cctgatgatg ccgcttccga tccactgaca tcactgccgg
     6421 atacaggcct gatcaggagc attaatgctg atttgtacaa tgcaggactg ggtattctga
     6481 tcctcactgt aaatccgtct gaagagcctg attgcattca cgaggttgcc aggctttttg
     6541 gtaaccacaa gcattcatca tctttcaggc tgattctggc agttgatcat accgtggatg
     6601 tgactgatct ttttatggtt gcatggcagg tcctggccaa ttccgatccg atcagggatc
     6661 acatatggat ttccgggtct gttttgttga tcgacggaac aataaagcat tacaggcaga
     6721 atgggtttcc cagaccctgg cccaacattg tctgttccga catggaaact ataaaggccg
     6781 tggatgaaaa atggcaggat ctgggatttg atggctttat tccatctccg tcacttaaat
     6841 atatccccct ggtcagaaaa ggaagggccc gcattgaaga ttgaatcggc tgaccgtaag
     6901 acagttttat ttgtgaatga caaatatctg tgcgatttgc ttgataccct gtcggagaaa
     6961 tggttatttt tgtgatatgg tatagttttt gatccggaat tattaagaac tcattagaat
     7021 gaagcaaaat ttcggaattc tgtttctggc agcttttatc tgtttcctgg ctggcggttc
     7081 cgtggcaaat ggccagcccc cggaaggcat ggtaaaattt actcctgatt tcaggttcgt
     7141 cgatggtgta tttctgaatt tcgatcaggt aaaagccaat gatcccatac caaaggcaaa
     7201 gcttcttact tccaccgatt ataacgacaa ggatttcttc aagaatctct tttccggcga
     7261 caaaatatat tattacgacg gtatgggcat caggcaggag gttgataaaa agacggtctg
     7321 gggatactcg aggaatggaa tcctc
//
LOCUS       DAOOYG010000136         7240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_11674_length_7240_cov_6.836326, whole genome shotgun sequence.
ACCESSION   DAOOYG010000136 DAOOYG010000000
VERSION     DAOOYG010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11674_length_7240_cov_6.836326"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PK288_09930"
     CDS             <1..215
                     /locus_tag="PK288_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19285.1"
                     /translation="KSIWPFILPVLGFLVCLYLWLSLGTKAKIVGICWLTLGVLYGAW
                     KTSWFRKPLQFAKIDNGEDSSAAAAS"
     gene            633..1118
                     /locus_tag="PK288_09935"
     CDS             633..1118
                     /locus_tag="PK288_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19286.1"
                     /translation="MKRMILAAIIAAGSGAAAFAGKFIAEGKSFTPLGSYRIELSDKH
                     FTMKGENCRTYIIKYENSPMEVTVAVRNEQNCRRYLVLSDKLSVQYVCKSNLFGVKKL
                     GKEFAKDGLVTEPGEINMNEYLHQKVISRGTWSEVEAAQLIASYFPMLFNDIDDVMAL
                     K"
     gene            complement(1187..1951)
                     /locus_tag="PK288_09940"
     CDS             complement(1187..1951)
                     /locus_tag="PK288_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM19287.1"
                     /translation="MTKKIRSVIIDDEPDAVDFINTIIGEFCTSLEVVGKACNVSQGV
                     EIINRTQPDLVFLDVEMPNGSGFDLLKHYPEKDFEVIFITAFNHYAIRAIKFSAVDYI
                     LKPININEFIEAVGRVIKKRTGKSHQSNESFRALMENLRSPMPSRLAIPTSDGMEYLN
                     PKDIIRIEADRSYSWFFIAGERKMLVSRHLKEFQELLGDRCFFRPHNSHLINLRFVKK
                     FIRRDGGYIEMSDGSLVPVSRNRKDIFLDKMSRFKG"
     gene            complement(1948..3840)
                     /locus_tag="PK288_09945"
     CDS             complement(1948..3840)
                     /locus_tag="PK288_09945"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPM19288.1"
                     /translation="MARKAECSPVSRLSSGIRSDTSAVNQLVREGFRYLDKPENIEKA
                     KQNLDSALLIYRGKNMEIPSQLHLLSAELLFISGDLGAAAGEAAFALEKSTNKREYSV
                     QAKTNIFFGKYYQRIGSYKESLESLDRAIAISRKYGLKGLDARSCLAKASVYESLGDR
                     DGLRSSLLNMINSGYEENDLRIVADGCYRLGTSFCGDSSLSAGRNFFRADSLFRVSLR
                     ISVALNDTSLISQAYANSGWNFYLEGMYDSSLANYNRSLRYSLPSRNYETSANSLGNI
                     GTIYRDLREYQESEKYYKKAVEQGNIADDVYNLRWIYEDMSKMYLDQEDTSKAFQAYV
                     RFKKYNDLYLMKSTTQGLTDVRVRYDADTHRKEAEMLTLMLKNHRLLIWGFAGLVILS
                     SFLGVLVYRQAQLRNKKRISEMKQKISEITQANLRQQMNPHFIFNTLNSIQYYMYQHD
                     RLATNTYLTKFANLIRKVLDNSRHTMVPLSDELDALTLYLELESIRFRDKFLYEINVD
                     REIDPLIYRVPAMLIQPYVENSICHGIVPLEETGIIRIDLKLSEDHILCTIEDNGIGR
                     AAARERSLSRERNYNSLGTQITSSRIDLMNELYGTSLKTVCTDLKNENGKPAGTRVEI
                     HIPVMT"
     gene            4185..5018
                     /locus_tag="PK288_09950"
     CDS             4185..5018
                     /locus_tag="PK288_09950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPM19289.1"
                     /translation="MKSINLTLKALMSASFLLFSVLVSGQQQSPAATFEPQVGQQGKD
                     VVWVPTPQELVNKMLEIAHVGPSDFVIDLGSGDGRTVISAAKLGARALGVEFNPDMVA
                     LSRSNARKEGMNHLAEFVEGDLFETDLSKATVITMFLLPEINLRLRPKLLELKPGTRI
                     VSNTLTMGDWQEDVEATTDENWNSWNQAHLWIVPAKVEGTWKTGNGELKLTQKYQMLE
                     GSYISGGKESRIREGRMKGDIISLTINDRIYTGRVQGNRIEGNIADSSGNSLREWTAT
                     R"
     gene            complement(5044..7056)
                     /gene="uvrB"
                     /locus_tag="PK288_09955"
     CDS             complement(5044..7056)
                     /gene="uvrB"
                     /locus_tag="PK288_09955"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPM19290.1"
                     /translation="MEFRLISDFQPTGDQPEAIKQLVDGLNKGVHYQTLLGVTGSGKT
                     FTIANVIEKVQRPALVLSHNKTLAAQLYGEFRQFFPENRVEYFVSYYDYYQPEAYIPV
                     TDTYIEKDLSINDEIEKLRLSATSALLSGRRDVIVVSSISCIYGIGNPDDLQSNTIHI
                     RQGQNIGRNHFLRKLVESLYSRNEMEFRHGTFRVRGDTVDIFPAYADQAVRLVFFGDQ
                     VEEISSFDPVSGQRIEILNEYIVYPANIFVTTRERINQAIRDIQDDLAGQVAHFIDIG
                     RKEEAKRLEQRVLYDLEMIRELGYCSGIENYSRYFDGRSPGTRPFCLLDFFPDDFITV
                     IDESHVSVPQIRAMYGGDHSRKQTLVEYGFRLPAALDNRPMRMEEFEALTGQTIMVSA
                     TPADYELEKSEGIIVDQVIRPTGLLDPPIEVRPSLNQIDDLMAEIRQRTGLGERTLVT
                     TITKRMAEELASYLMKYDIRCRYIHSDIDTLERIDIMEGLRSGTFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSARSLTQTAGRAARNLNGKVIMYADTVTGSMQETIDET
                     NRRRAKQMKYNEEMGITPSQIIKKTGTILSGLSRKGVNVRAYTGPERIDLAADPVVRY
                     MSRQALEKAAEKTRKSMEKAAAELDFIQAARFRDELAELQKLLKSR"
BASE COUNT         1893 a         1684 c         1583 g         2080 t
ORIGIN      
        1 agaaaagtat ctggccattc atccttcccg tactgggctt tcttgtgtgc ctctacctgt
       61 ggctcagcct tggcaccaaa gccaaaattg tcggaatttg ctggctcaca ctcggagtgc
      121 tctatggtgc atggaaaaca tcgtggttca gaaaaccgct ccagtttgca aagatcgaca
      181 acggagagga ttcatcagct gctgccgcat catgatgaag tcccacacag gctgatatgc
      241 aaaaacatgc aattcagcct tgcttccggg gaaatacatg ctcatatact gtcccttccg
      301 gatcaggtta tcttttaatc atatctgact gttacccgga ttgataggtt ctgcttcctt
      361 tttccattca cctgacacca ggaaacatga gaaatccgta ccggtatgtt ttctgacccg
      421 gaccaacggt gaaatctcaa atccagccgt catgttttta accattcgtt aattcaaacc
      481 gggtatccgt tgaatatgtc ttttctgcta accgctgaaa acataatttc gggataggat
      541 ttaaaaaact ccggggaatg aatatccgtc cggacaaacg gatagaaaaa gtgagttccc
      601 tgtattatta attttaatta aaacattaaa ccatgaaaag aatgattttg gcagcaatta
      661 ttgcagcagg atccggtgct gctgcatttg caggcaaatt tattgccgaa ggaaagagtt
      721 ttaccccgct gggtagctac aggattgaat tgtctgacaa gcactttacc atgaaaggag
      781 agaattgcag aacttatatt atcaaatatg aaaactcacc catggaagtt acggttgcag
      841 ttcgcaatga acaaaattgc cggagatatc tcgtactgtc cgacaagctt tctgtacagt
      901 atgtttgcaa aagcaatctt ttcggagtca agaaactcgg aaaggagttt gcgaaagatg
      961 gcctggtcac tgaacccggg gaaatcaaca tgaatgaata tttacatcag aaagttatca
     1021 gtcggggtac atggagtgag gttgaagccg cccagctaat tgcttcttat tttcccatgc
     1081 tgttcaatga cattgacgat gtcatggcat tgaaataaga tcttaaaggc cgtgaggctg
     1141 ctaagtttag gtttgaattg aaaaggggcc cgctgcgggt ccctttttaa cctttaaatc
     1201 tcgacatctt atccagaaaa atatctttcc tgtttcttga aaccggtacg agcgatccgt
     1261 ccgacatttc tatataaccc ccgtccctgc ggataaattt cttcacgaat ctcaggttta
     1321 tcaggtgtga attatgagga cggaaaaaac agcggtcgcc gagaagctcc tggaactcct
     1381 tcagatgccg tgaaaccagc atcttacgct cgccggcaat gaagaaccat gaatagctcc
     1441 tgtcggcttc aattctgatt atgtccttcg gattgaggta ttccattccg tctgaagtcg
     1501 gtattgccag ccgtgagggc atgggcgacc tgaggttttc catcagtgcc ctgaagcttt
     1561 catttgactg atgagacttc cccgttctct tttttattac cctgccgaca gcttcaatga
     1621 actcattaat gtttatgggt ttcaggatat aatcgactgc gctgaatttg attgccctga
     1681 tcgcatagtg gttgaatgct gttataaata ttacctcgaa atccttctcg ggataatgct
     1741 tgagcaggtc aaagcccgat ccattcggca tttcaacatc caggaagact agatcaggct
     1801 gagttctgtt tattatctcc acaccctggg aaacattgca ggcctttccc accacttcca
     1861 ggctggtgca gaattcgccg ataatagtgt tgatgaaatc aaccgcatcc ggttcatcat
     1921 cgataattac actcctgatc ttttttgtca tgtcataacg ggtatatgta tttcaaccct
     1981 ggtgcctgcc ggctttccat tttcattttt cagatcggta cagaccgttt tcagacttgt
     2041 tccatagagt tcattcatca gatcaatccg tgaacttgtg atctgagtgc cgagagaatt
     2101 gtaatttctt tccctgctga ggcttctctc cctcgctgcc gcccggccaa taccgttatc
     2161 ctcgattgtg cataaaatgt gatcctcact cagtttcagg tcaatcctta tgatccctgt
     2221 ctcttccagc ggtacaatac cgtgacagat tgaattctcg acatagggtt gtatcagcat
     2281 tgcggggacg cggtagatca acggatctat ttccctgtcc acattgatct catagagaaa
     2341 tttgtccctg aaccttatac tttccagttc gaggtaaagg gtaagggcat caagttcatc
     2401 actgagcggc accatagtat gtcgtgaatt atccagcacc ttgcggatca gatttgcgaa
     2461 tttggtcagg taggtatttg tagccagcct gtcgtgctga tacatgtaat actgaatgga
     2521 attaagggta ttgaatatga aatgcggatt catctgctgc ctgagatttg cctgggtgat
     2581 ttcagaaatc ttctgcttca tttcactaat cctttttttg ttcctgagct gagcctgtcg
     2641 ataaaccaga acccccagaa atgatgacag aataaccagt ccggcgaagc cccatatcag
     2701 gaggcggtgg ttcttcagca tcagggtgag catttctgct tccttcctgt gtgtgtcggc
     2761 atcgtacctt acgcgcacat cggtcaatcc ctgggttgtg gacttcataa gataaaggtc
     2821 gttgtacttt ttaaacctga cataagcctg aaaagccttt gaagtatcct cctggtccag
     2881 atacatcttg ctcatatcct cataaatcca tctgagatta tatacatcat cagctatatt
     2941 tccctgttcg acagcttttt tataatattt ttccgactcc tggtattccc gcagatccct
     3001 gtaaattgtg cctatgttgc ccagtgagtt tgcggaagtt tcgtaattcc gggagggaag
     3061 actgtatctg agtgaacggt tataatttgc aagcgatgaa tcatacattc cctcaagata
     3121 gaaattccaa cctgagtttg cataggcttg tgaaatcagg ctggtgtcat tcaatgcaac
     3181 cgatatcctg aggcttaccc tgaacaacga gtcggccctg aagaaattcc ttccagctga
     3241 caaagatgaa tctccgcaaa atgatgtacc cagtctgtaa caaccatcag caacaatccg
     3301 aagatcgttt tcctcatagc cggagttaat catattcaag agactactgc gaaggccgtc
     3361 cctgtcaccc agactttcat agacagaggc tttagcaagg caggacctgg catcaagtcc
     3421 tttcagtccg tattttcttg aaattgcaat ggcccggtca agactttcaa ggctttcttt
     3481 gtatgaaccg atgcgttgat agtattttcc gaagaaaata tttgttttcg cctgaacaga
     3541 atattccctt ttatttgttg acttttccag ggcaaaggct gcttctccgg cagcagcgcc
     3601 caaatcaccg gaaataaaca gaagttccgc ggaaagaaga tgcagttgtg atggaatctc
     3661 catgtttttc ccccggtaaa tcagaagggc actgtcaaga ttctgctttg ccttttcaat
     3721 gttttcaggt ttgtcaagat acctgaagcc ttccctgaca agctgattca ctgctgacgt
     3781 atctgaacgg atccccgaag acaatcgtga aaccggggaa cactccgctt tccttgccat
     3841 atcccggata caggatgtat gattgcaggc tggcaataac aggatgatca gatatacgac
     3901 agctttcatt tatttgttgc caaagaatat ttgcagctga aatgaatatt tccaggtatc
     3961 tgaaatgtac tttataaacc gatctctcat cgctgatata accaagcaat ttacgaatta
     4021 agcatggaat aagatgtaaa tcttaacgga tggctatttt gagtcaacaa aatacaggtt
     4081 tctgacaata attgtaaccg taaataataa attccaatta tttgttaaat ttaatcccat
     4141 tttcatattc aggctgttta ttatataatc tcaaattaac acccatgaaa tcaatcaatt
     4201 tgacattgaa ggcattaatg agtgcatcat ttttgctttt ctcggttctg gtcagcggcc
     4261 agcagcaaag tccggcggct acatttgaac cgcaggtcgg gcagcaaggc aaggatgtgg
     4321 tatgggttcc cactccccag gaacttgtga ataaaatgct ggaaatagca catgtcggtc
     4381 cgtccgactt tgttattgac cttgggtcgg gagatggaag aacggtgatt tcagctgcaa
     4441 aactgggagc cagggcgctc ggcgtagaat tcaatcctga tatggttgca ctttcaagga
     4501 gcaatgccag gaaagaaggt atgaatcatc ttgcggaatt tgtcgaaggc gatctctttg
     4561 aaaccgatct ttcaaaggca acagtgatta ccatgttcct gctgccggag atcaatctca
     4621 ggctccgccc gaaactgctt gaactgaaac ccggtaccag gattgtttca aacacattaa
     4681 caatgggcga ctggcaggaa gatgtggaag caaccaccga cgagaactgg aacagctgga
     4741 accaggctca tttatggatc gttcccgcaa aggttgaagg aacatggaaa acgggcaacg
     4801 gcgaacttaa gctgacccag aaataccaga tgctggaagg ttcttatatt tcaggaggga
     4861 aagaatccag gatcagggag ggacgcatga agggagatat aataagcctg actatcaatg
     4921 atcgtatata taccggccgg gttcagggta atagaataga gggcaatatt gcagacagtt
     4981 cgggcaacag tcttagggaa tggacagcca cccgctgaat ggttgctcta ccggtattgt
     5041 gatttacctg gattttagca gtttctgaag ttcggcaagt tcatccctga atctggcagc
     5101 ctggatgaaa tcaagctcag cagcggcctt ttccatactt tttctggttt tttcggctgc
     5161 tttttccagt gcttgcctgc tcatgtatcg aacaaccgga tcagcagcca gatcaatacg
     5221 ctccggcccg gtataggccc tgacattgac ccctttacgg ctgagcccgc ttaaaattgt
     5281 accggtcttt ttaataatct gcgaaggggt aattcccatc tcttcattgt atttcatctg
     5341 ctttgccctt cggcggtttg tttcatcgat cgtttcctgc atgcttcccg tcactgtatc
     5401 tgcatacatt attaccttgc cgttaaggtt ccttgcagct cttcccgcag tctgggtcag
     5461 tgatcttgct gatctcagga atccttcctt gtcagcatcg agtattgcca caagcgaaac
     5521 ttcaggcagg tcaaggcctt ccctcagcag gttgaccccg accagcacat caaaggtacc
     5581 gctcctgagt ccttccataa tatcgatccg ttcaagggtg tcaatatcgg aatggatgta
     5641 gcggcacctg atatcatatt tcatgagata tgatgccagt tcttcggcca ttcgcttggt
     5701 gatggtggta acgagggttc tttcgcccag gccggtcctc tgcctgattt cagccataag
     5761 atcatcgatc tggttaagac tgggtctgac ctctatcgga ggatccagga gacccgttgg
     5821 cctgatcacc tgatctacaa tgatcccttc acttttttca agttcataat ctgccggagt
     5881 ggcgcttacc attattgtct ggcccgtcaa ggcctcaaac tcctccatac gcatgggtct
     5941 gttatcaagt gcggcaggaa gcctgaaacc atattccacc agggtctgtt tcctcgagtg
     6001 gtcgccgccg tacatggccc ttatctgcgg tacgctcacg tgactttcat cgataacggt
     6061 aataaaatca tccgggaaaa aatccagcag gcaaaagggc ctggtcccgg gactacggcc
     6121 gtcaaaataa cgcgaataat tttcaatgcc gctgcagtat ccgagttccc tgatcatttc
     6181 aagatcgtaa agaaccctct gctcaagtct tttggcttct tccttcctgc caatatcaat
     6241 aaaatgcgcg acctgtcctg caaggtcatc ctggatatcc cgtatggcct gattgatcct
     6301 ttcacgtgtt gttacaaata tgttggccgg gtaaacgata tattcattta gtatttcaat
     6361 ccgctggccg ctcacaggat caaaggagga gatctcctcg acctgatccc cgaaaaaaac
     6421 cagtcgcacc gcctggtccg cgtaagcagg aaaaatatca acggtatcac cccttaccct
     6481 gaatgttccg tgcctgaact ccatctcatt tcttgaataa aggctttcaa caagcttcct
     6541 gaggaaatga ttcctaccta tgttctgccc ctgccttata tgaatggtgt tcgactggag
     6601 atcatccgga ttgccgatac cataaatgca tgagattgaa gatacaacaa taacatcccg
     6661 cctgccggaa agaagggccg atgtggcgct gagcctaagt ttttcaattt cgtcattgat
     6721 cgacaggtcc ttttctatgt aggtatcggt aaccgggata tatgcttctg gctgataata
     6781 atcgtaatat gacacaaagt attcaaccct gttttcgggg aagaactgcc ggaactcacc
     6841 gtaaagctgg gctgcaaggg ttttattatg gctgaggacc agtgccggcc gctgtacttt
     6901 ttctattaca ttggcaatgg tgaatgtctt acctgatccg gttacaccaa gcagtgtctg
     6961 atagtgcact cccttgttca atccgtctac cagctgcttt atcgcttccg gctggtcacc
     7021 cgtcggctga aaatccgata tgagcctgaa ctccatttaa gtgcaaaatt agccgttctt
     7081 ctcctattat tgaatcatat ttatcaaata ctggcaaccc tgaaattctg tcaaaagtat
     7141 ttgttttttg atcaaaaatg tctggatttt ttaatttcta attagtatca atagttcgtt
     7201 aaagttttaa gcggccatag tgccgtataa tataagttct
//
LOCUS       DAOOYG010000137         7235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_11684_length_7235_cov_5.398050, whole genome shotgun sequence.
ACCESSION   DAOOYG010000137 DAOOYG010000000
VERSION     DAOOYG010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11684_length_7235_cov_5.398050"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="PK288_09960"
     CDS             <1..1265
                     /locus_tag="PK288_09960"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19291.1"
                     /translation="SHRWSDIIKGGHNAEVKLFITDNAESAGSLGSICRKNISERQGI
                     ESLEAAAVIRAVENNRDAIGFCQLNDVIAAGVSGTNGNIRFMPVDKNSNGRIDGFEDI
                     YANTEELARGIWIGKYPKALCSNICAVAAEKTPGETIIAFLSWIMNDGGRYLAAFGFS
                     ELPGFGKESNMAILAGTGQTASVAAKHSGSGLWILLLSLVALSGVFLIMVFGARRRTR
                     ILIPSGATVSDRPFTDTAVNVLKGIYYDRSHTWSFMEKDGSVRIGIDDFLQHVTGDLT
                     KVRLREHGEHVRRGEKIFSLVRNGKQIDIHSPVSGIIKEKNISLGEDSSSINRSPYGD
                     GWVYLIEPVNWEKEIRYMFMAEKYREWIRDEISRLKDFLAASFVQHKLAYAHAVLQDG
                     GELTDNVLAELEPEIWEDFQRNFLDKTA"
     gene            1452..3122
                     /locus_tag="PK288_09965"
     CDS             1452..3122
                     /locus_tag="PK288_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPM19292.1"
                     /translation="MFRTGIQSRKSKATTSILDSYVRFRSSIYGRVVFIITITSILLF
                     ISFWMIFRSVNERYMKSILEENGHNIGSLVEGALYKSMLENDRTSLQGTLDIINTMTG
                     IDDVNMYDTDYNLAYSSISGDDEKHIDPDCKSCHADIAAMFPKKEKAYRIIDSDSECK
                     MSRKTNNGRHLLISSPILNAPSCYTADCHAHDQTEEVLGSLLIKLPLKGIDDSLDRSR
                     KNYFIIASVMTALLLIMLVVFTGEKIKKPLHSLIRASEAVSRGDNTTRLEIRPNQLSD
                     MRMVSAAFNHMLDNLQKASEELQNWSQQLEYKVRKKSEELGQAQNELMNVERIASLGR
                     LSLSVAHEINNPLSGILIYAKLIYKQLQNQELPKEKKESVLKHLKFIESEAKRCGDIV
                     KGLLDFSRKDQNDFEPRNINPILLETHDLMEHQMKVANIRFLHDFSAGNDLVYCSPNQ
                     IKQVCLALLVNAAEAIGENGEVVFSSTNPDERHIRIDVTDNGPGISNDDMPHIFEPFY
                     TTKDKGNGIGLGLAIVHGIIQNHKGRTEVRSERGSGTTMSVILPLINS"
     gene            3134..4474
                     /locus_tag="PK288_09970"
     CDS             3134..4474
                     /locus_tag="PK288_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPM19293.1"
                     /translation="MDHGISILIVDDELSVRDSLYNWFIEDGYTVECAEDAKKALSIL
                     ESQDFDIILADIKMPGMDGMEMLRRIKSIKKDSIVIMMTAFATVDTAVQALKEGAFDY
                     VTKPFDPDDLTHLIRNASKQITLSEENKILKDRVTALENVDNLIGNSEAMQQLLRDIQ
                     SVAQSNASVIITGESGTGKELVARAIHANSPRRFFPMISVHCGALTESLLESELFGHE
                     KGAFTGAVYNRKGRFEMADNGTIFLDEIATISVKMQVELLRVLETKSFVRVGGTKEIK
                     SDFRVICATNRDLRSMVDKGLFREDLYYRLNVVNIHVPPLRERTEDIPILAEYFAGKY
                     CSAMGKPPVTFDPAAIKKLGEFPFPGNIRELENMIERAIVVGNGKRIQVKDLPIGTSS
                     AGSSFESLEELEKNHIGNILEKYRWNISAAAKALKVDRVTLYNKIKKYGLNQNG"
     gene            4547..5083
                     /locus_tag="PK288_09975"
     CDS             4547..5083
                     /locus_tag="PK288_09975"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HPM19294.1"
                     /translation="MNYENITLIAFGLPDQDLLKAVAGVISGSLPLPLLLKSGYLDLS
                     EFYDPARNQYNGNSILKKLDDDYSLDSSKTMGLFAVDLYIPILTYIFGQAHLNGNTGI
                     VSIYRLTNERYGIGKNGRLLVGRLGKEIIHELGHMFGLIHCSDPVCVMRSSTYVEDID
                     QKESRFCKACRTILNTRL"
     gene            complement(5143..5715)
                     /locus_tag="PK288_09980"
     CDS             complement(5143..5715)
                     /locus_tag="PK288_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19295.1"
                     /translation="MKIRAWLLFFVLFFTSQAVRSPEIDFQTKFARIRRVLNYLERQH
                     YEMEFFRFITDLGRSESLNNWFCINRIGCFGEWQFAEATLHCLGFRHITLQKFRQDPW
                     IFPREMQQKALESLIRVNLALLRDYEHFIGDTINGTVVTKSGLIAAAHLGGAGSTKRF
                     LRSGGFVNNKDALGTSIQDYLRKFRDYDLE"
     gene            complement(6129..6551)
                     /locus_tag="PK288_09985"
     CDS             complement(6129..6551)
                     /locus_tag="PK288_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPM19296.1"
                     /translation="MSTEENIRKIAAIILENEHRELLLYRRDNKPGIPFPGHWDLIGG
                     HVENGETPEKALVREVKEELDLDLKEFSFFKKYECFEGDAYPNIKYIYYGKINLPVEK
                     ITLLEGDIVRYFKREEIADVKFANILKGIVLEYLSAGF"
     gene            complement(6619..>7235)
                     /locus_tag="PK288_09990"
     CDS             complement(6619..>7235)
                     /locus_tag="PK288_09990"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPM19297.1"
                     /translation="DGTLVFPAQFKANTGEKALDGGIFTPFSTIVYSRDGGETWTIGT
                     GAKSNTTEAQVVELPDGSLMLNMRDDRNRTEKGSANGRAVYTSADMGTNWTVHPSSNS
                     VLTEPNCMASIIASDIGINGKLKRVLFFSNPDHDSERINMTIKASLDNGFTWPGEFRL
                     LLNGAAGYGYSCLTMVNDSTLGIVYEGMKDLYYQKIRVRDVLNN"
BASE COUNT         2087 a         1626 c         1654 g         1868 t
ORIGIN      
        1 ggagtcacag gtggagcgac ataatcaagg gtggccataa tgctgaagta aagctgttta
       61 tcaccgataa tgcagaatct gcaggatcac ttggcagtat ctgccgcaaa aatatctcag
      121 aacggcaggg cattgaatct ttggaggcag cagcagttat cagggctgtt gagaataaca
      181 gggatgcaat aggtttctgt cagctcaatg atgtaattgc agccggagtc tcaggaacga
      241 acggcaatat caggttcatg ccagtggata agaactccaa cgggaggatt gacggttttg
      301 aggacatata tgcaaatacg gaagaactgg caaggggaat atggatcggg aagtatccca
      361 aagccctttg cagcaatatc tgtgctgtcg ctgcggaaaa aacccctggc gaaacaataa
      421 ttgcctttct ttcatggata atgaatgacg gaggaagata cctggcagct ttcggtttca
      481 gtgaacttcc cggttttgga aaagaatcca acatggcaat tctcgcagga acgggccaga
      541 ccgcctcagt tgctgcaaaa cattccggtt caggtctgtg gatcctcctg ctctctctgg
      601 tggcactttc aggcgttttt ctgatcatgg tcttcggcgc caggagaagg accaggattt
      661 taattccttc aggtgcaact gtcagcgacc ggcccttcac ggatacagca gtaaacgttt
      721 taaagggaat ctattatgat agatcgcata cctggtcgtt catggaaaag gatggcagcg
      781 tgaggatcgg catagacgac ttcctccagc atgtaacagg agatctgaca aaggtcaggc
      841 tcagggagca tggggagcat gtcaggagag gcgagaagat attctctctg gtaaggaacg
      901 gcaaacagat tgatattcac tcacctgttt caggtatcat aaaagagaaa aatatttcgc
      961 tcggcgaaga ttcctcctct ataaacagat caccttacgg tgacggttgg gtatatctta
     1021 tcgagccggt caactgggaa aaggagatcc gttacatgtt catggcagaa aagtaccgtg
     1081 aatggatcag ggatgaaatc agcaggctga aggatttcct ggctgcatcc ttcgttcagc
     1141 ataagcttgc ctatgcacat gcagttctgc aggacggagg cgagctgacc gacaatgttc
     1201 ttgcagaact tgaacctgaa atatgggaag atttccagag gaattttctc gacaaaacgg
     1261 cttaggcaat acaatttttc gcaggcatgt tcataagatt atgcctgcga ctgatccagg
     1321 cacagggggg gagacggaaa acgttaccta ctgtgccttg atcatattca tattattata
     1381 tttgattccg ggaataagtc cctatcaaat attgcagtat cttgtgatgg ataaaaagca
     1441 gaaaccgata tatgttccgt acgggaatac aaagtcgtaa gagtaaggca acgaccagca
     1501 ttcttgacag ttacgtcaga ttcagatcct ccatttacgg cagggtggtt tttattatta
     1561 ccattacatc cattctactg ttcatatcat tctggatgat cttcagatcg gtcaatgaac
     1621 gttacatgaa gtcgatcctg gaagagaacg gtcataatat cggatcgctt gttgaaggtg
     1681 ccttgtataa atcgatgctg gaaaacgaca ggacttctct acagggcact cttgacatta
     1741 tcaacacgat gacggggatc gacgacgtga acatgtatga taccgattac aatcttgcct
     1801 actcatctat ctccggagat gatgaaaagc atatagatcc tgactgcaaa agctgtcatg
     1861 ccgacattgc agccatgttt cctaagaaag agaaagccta tagaataatt gacagtgaca
     1921 gtgagtgcaa gatgagccgg aaaacgaata acggccggca tctgctaatc agttcaccga
     1981 ttctcaatgc gccttcctgc tacactgccg actgtcatgc ccatgatcag actgaggagg
     2041 ttctgggctc tcttctgatt aagcttccgc tgaaaggcat cgacgattca ctcgacagat
     2101 cgaggaagaa ttattttatt attgcgtcgg tgatgactgc cttgttgctc ataatgctgg
     2161 ttgtattcac cggtgaaaag atcaaaaagc ccctccactc cctgatcagg gcaagtgagg
     2221 ccgtttcaag gggagacaac acaaccaggc ttgagatcag gcccaatcag ctttctgaca
     2281 tgcggatggt ctcagccgca ttcaatcata tgctcgacaa cctgcagaag gcatccgaag
     2341 aacttcagaa ctggtcgcag cagcttgagt ataaggtcag gaaaaagtca gaggaactcg
     2401 gccaggccca gaacgaactg atgaatgttg aaaggatagc ttcccttggc aggctgtctc
     2461 tatcggtggc tcatgaaata aataatccgc tgtcaggaat ccttatttac gcaaagctca
     2521 tatataagca acttcagaat caggagctcc cgaaagaaaa aaaggaatcg gtacttaaac
     2581 acctgaagtt tattgaaagt gaagccaaaa gatgcggcga cattgtcaag gggctgcttg
     2641 atttctcgcg caaggaccag aatgattttg aacccaggaa cattaatccg atattgcttg
     2701 aaacccatga tctgatggag catcagatga aagttgccaa tatacgtttc ctgcatgatt
     2761 tttcagcagg aaatgacctg gtatattgca gccccaacca gatcaagcag gtttgcctgg
     2821 ccttgcttgt caatgccgcc gaagcaatcg gcgagaacgg ggaggtggtt ttcagcagca
     2881 ccaatcctga tgaaaggcac atacgcattg atgtgactga taacggaccc ggcatttcaa
     2941 acgatgatat gccccacatt tttgagccct tttacaccac caaggacaaa ggcaacggta
     3001 ttggattggg actggcaatt gtccatggca tcattcagaa ccataaaggg agaaccgagg
     3061 tcaggtcaga aagaggcagc ggtaccacta tgtcagtcat cctgccatta attaacagtt
     3121 aaaggaacta attatggatc acggtatctc aatacttatt gtcgatgacg aattatctgt
     3181 cagggattcg ctttacaact ggtttattga ggacggttac actgtagaat gcgccgaaga
     3241 cgcaaaaaag gctttgtcga tcctggaatc gcaggatttc gatatcatac tggccgacat
     3301 caaaatgccc ggaatggacg gaatggaaat gctacgacgc ataaaatcga taaaaaagga
     3361 ttccatagtg atcatgatga cggcttttgc cacggtcgat acagcagtgc aggccctgaa
     3421 agaaggtgct ttcgattatg taacgaaacc ttttgatccc gacgacctta cccatctcat
     3481 aagaaatgca tcaaaacaga taaccctgtc cgaagaaaac aagatcctga aggaccgtgt
     3541 cacggctctt gaaaacgttg ataacctgat tggcaacagc gaagcaatgc aacagctgct
     3601 gagggatata cagagcgttg cgcaatcaaa tgcctcagtt ataattacag gtgagagcgg
     3661 caccggcaaa gaactggtgg ccagggcaat acatgcaaat tcaccccgca ggttctttcc
     3721 aatgataagc gttcattgcg gcgcattaac cgaaagcctt ctggagagtg aactgttcgg
     3781 acatgaaaag ggggccttca ccggcgccgt atacaacagg aagggaaggt tcgaaatggc
     3841 tgacaacggc acaatattcc tcgatgaaat tgccaccata tccgtgaaga tgcaggtgga
     3901 gcttctcagg gtactggaaa ccaaaagttt tgtacgtgtc gggggcacaa aggagatcaa
     3961 atcagacttc agggttatat gtgcaaccaa cagggatctc aggagtatgg ttgacaaggg
     4021 ccttttcagg gaagacctgt actacaggct gaatgttgta aatatccacg tacctcccct
     4081 tcgcgagaga actgaggata ttccaatact tgcagaatat tttgctggca aatactgttc
     4141 ggcaatgggc aagcctcccg taacattcga tcccgcagcc atcaaaaaac tcggggaatt
     4201 cccgtttccc gggaatatcc gggaactgga gaatatgatc gaaagggcaa tagtggtcgg
     4261 aaacggcaaa cggatacagg taaaggacct tcccattggc acttcatcag ccggctcctc
     4321 gtttgagagc ctcgaagagc ttgaaaagaa tcacatagga aacatcctgg aaaagtacag
     4381 gtggaacata tcagctgcag ccaaagccct gaaagttgac cgggtaaccc tttacaacaa
     4441 gataaaaaaa tacggattga atcagaatgg ttaatcttcc tgcgggaccg aatccaatct
     4501 caatcaatct ggatgatcct gtttcatgct ccaaaacagc gggttgatga attatgaaaa
     4561 cataacattg atagcatttg ggcttcctga ccaggatctt ttaaaagcgg tcgcgggagt
     4621 aatatccggc agtcttcctc ttcccctcct gctgaagtca gggtatctcg atctgagtga
     4681 attttatgat cccgccagga accagtataa cggaaattca atcctgaaga aactggatga
     4741 tgactattcc cttgattcat ccaaaacaat gggacttttt gccgtagacc tatatattcc
     4801 cattcttacc tatatcttcg gacaggctca tctgaacgga aacaccggaa ttgtttccat
     4861 atacaggcta accaatgaac ggtacgggat cggcaaaaac ggcagattgc tggtagggag
     4921 gctgggcaaa gagatcattc atgaactggg acacatgttc ggactgattc attgttccga
     4981 tcctgtatgt gttatgagat ccagcactta tgtcgaggat attgaccaga aagaatccag
     5041 gttctgtaag gcctgcagga ctattttaaa tacccgtttg taattcagcc agccttgttg
     5101 tatgcccgat ccgggcaacc ttttctttta ataattctga aatcactcca ggtcgtaatc
     5161 cctgaatttc ctcaaatagt cctggattga agttccaaga gcatctttgt tgtttacgaa
     5221 accacctgac ctgaggaatc tctttgtcga tcctgcccct ccgagatgtg ctgccgcaat
     5281 aagccctgat ttggttacaa cggtcccatt aatggtatcg ccgataaaat gctcataatc
     5341 ccttaacaga gccaggttta ccctgatcag cgattcaagg gccttttgct gcatttcacg
     5401 ggggaagatc cagggatcct gcctgaattt ttgaagtgta atgtgcctga atcccaggca
     5461 gtgcaatgtc gcctctgcaa actgccattc accgaagcag ccaatccggt taatgcaaaa
     5521 ccagttattc agcgattcac tgcgtcccag atcagttata aacctgaaaa actccatttc
     5581 ataatgctgc ctttccagat aattcagtac ccgcctgatc ctggcaaatt ttgtctgaaa
     5641 atcgatctca ggcgatctca ccgcttgtga tgtgaaaaac aaaacaaaaa acagaagcca
     5701 tgccctgatt ttcatttcct taattattaa gtaatttacc cgttcagtta tgatataaaa
     5761 ataatactga aactgatcgg tcagggcatg caatattcta tcagtgagtt aattaagtgt
     5821 ataaacccta ttctatgtca ccactttccg gttattcgct gacttatttg attcattggt
     5881 aaaaaaggta tctgcgttca tcaaaatcca ggggaatttt gcaggaaaat cagggcttaa
     5941 atccccttac cggaccaggc taactgataa cactaagaaa atgtgttaaa tacaaggaat
     6001 atcaaccatg aatctccgga tattccgaaa aatcatattt tgacaaacaa ccgaagtttt
     6061 atgattaaaa ctgaagggaa aacaacaata tagtcatatt attggatctg cgcagggtgc
     6121 cggttccatt aaaagccagc cgaaagatat tcaagtacaa ttccctttaa tatgttggca
     6181 aatttcacat cagcaatctc ttccctcttg aaatacctga caatatcgcc ttcaagaagc
     6241 gtgatcttct caaccggcag attgatcttt ccgtaataga tgtattttat attgggatag
     6301 gcatcgcctt caaaacattc atatttctta aaaaaggaaa actccttgag atccagatcc
     6361 agttcttcct ttacctccct gacaagtgcc ttttcgggag tctctccatt ctccacatgc
     6421 cctcctatca gatcccagtg acccgggaag ggtattcccg gcttattatc acgcctatac
     6481 agcagcagtt cccgatgttc attctccagg attatggctg cgatcttcct gatattctct
     6541 tcggtactca ttttcttatt tccactgcat ctttgttttt ctcttttcat caagttggct
     6601 tagcaggaga tgattcagtc agttattcaa aacatctctc actcttattt tctgataata
     6661 caggtctttc atgccttcat aaacaatccc cagcgtactg tcattcacca ttgtaagaca
     6721 tgaatagccg taacccgcag caccgttgag aagcagcctg aattccccgg gccatgtgaa
     6781 gccgttatca aggcttgctt tgattgtcat attgatcctt tcggaatcat ggtcagggtt
     6841 tgaaaagaaa aggactcttt tcagcttgcc gttaatacct atgtcggatg ctatgatgct
     6901 ggccatgcaa tttggctcag tcagaacgga attggaagaa ggatgcacgg tccagttagt
     6961 tcccatgtca gccgatgtgt aaacggccct tccattggca gatccctttt cagtccggtt
     7021 acggtcatcg cgcatgttaa gcataagaga accgtcaggc agttcaacaa cctgtgcctc
     7081 cgtggtattg cttttggcac ctgtacctat tgtccaggtt tcgcctccat ccctgctgta
     7141 cacgatggtt gagaaagggg taaatatacc gccgtcaagg gccttttccc ccgtattcgc
     7201 tttaaactgg gcaggaaaga caagtgttcc gtcgg
//
LOCUS       DAOOYG010000138         7145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_11891_length_7145_cov_6.076446, whole genome shotgun sequence.
ACCESSION   DAOOYG010000138 DAOOYG010000000
VERSION     DAOOYG010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11891_length_7145_cov_6.076446"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /gene="yajC"
                     /locus_tag="PK288_09995"
     CDS             <1..188
                     /gene="yajC"
                     /locus_tag="PK288_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPM19298.1"
                     /translation="KQKEMNNYRSSLKKGDKVVTTGGIYGRVYEVKDNYVMLEVGGDM
                     KLKVDKNALLKDPTAND"
     gene            209..1216
                     /locus_tag="PK288_10000"
     CDS             209..1216
                     /locus_tag="PK288_10000"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HPM19299.1"
                     /translation="MYGKILIWLRNKAGRGISNINRDYTIFGFFLLLSFIFWYLNSLG
                     KEIETEIRYPVRYVNLPKERTLAEELPSRLNLYLKGPGYAVLKLKLTGSRAPVILDIS
                     ALNYRRVPGSRDLSYYLKTSGIIPKLKNDLRSECQILYIKPDTLFFRFDRIVSKKVPV
                     VSAVEVITDRQYFIKGGIAIEPETIKISGPSGILDTITEVRTKYRKFTRVNETLKKSI
                     SLDLPDYYSASVKKVSVTIPVEQFTEAELTVPVKILNQPDSIDLKIFPDVVTVKGLVA
                     VNDFKKLAEIPFEVILDMDKVNLRSSERIPLEIRNVPPFLTSLRITPGDVDFLIEKNK
                     K"
     gene            1213..1824
                     /gene="coaE"
                     /locus_tag="PK288_10005"
     CDS             1213..1824
                     /gene="coaE"
                     /locus_tag="PK288_10005"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPM19300.1"
                     /translation="MKKPVNTVFRLGVTGGIGSGKSTVCKVFCALGVPVFYADHEARV
                     IMDNDTDIMGKINSAVGRDMYRNGSLDRVELAKLIFNDEILLGKINSIVHPAVFDRFA
                     DWYAGQAAPYVIMEAAILFESGASELVDKVLTVVTPVEERIERMIRGKKFTRQQVIER
                     INNQMDDETRIRNSDFLIYNSDNDMIVPAVLKVHNEILQMVKQ"
     gene            1831..2559
                     /locus_tag="PK288_10010"
     CDS             1831..2559
                     /locus_tag="PK288_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HPM19301.1"
                     /translation="MGRFAKWIGGGLGFVMGGPIGALLGFLVGSMVDSTVSGQVTYTQ
                     PQRQTARGDFSVSLLVLIAAMMKADGKVVKAELDFVKQFFLRQFGPESAREALRMLRD
                     ILKQDIPVRDVCLQINRNMDYSSRLQLIHLLFNISVSDAAVHPVELEAIERMSGYLGI
                     SGSDFKSIKNMFVPETDSAYKILEIDPGATDDEIKKAFRKMAMKYHPDKVSHLGEEFK
                     KVAEEKFKKVNEAYERIKKERNII"
     gene            2626..3126
                     /locus_tag="PK288_10015"
     CDS             2626..3126
                     /locus_tag="PK288_10015"
                     /inference="COORDINATES: protein motif:HMM:NF037329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HPM19302.1"
                     /translation="MVLKDIVAISGQQGLFRFIAQGKNSMIVEHLETGKRSSAFSSAR
                     VSSLEEISVFTDSEDMPLGKVFDRIYEKENGGEAIDPKSDPETLRAYFREIVPDYSKD
                     KVYNSDIRKVIMWYNILQRKNLLIPEEPEPASGEGADAAAAEEKAAGEGNSAGEAGNK
                     KEKDGE"
     gene            3123..3977
                     /gene="speB"
                     /locus_tag="PK288_10020"
     CDS             3123..3977
                     /gene="speB"
                     /locus_tag="PK288_10020"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPM19303.1"
                     /translation="MSRSCPNFGGEEVVYDYSGSGIIIVPVPYDKTSTWMRGADKGPA
                     AIMEASANLEFYDAETSSEVHLLGIHTVDPVTEEETPEKLVDAVFNQVLSIIDDGKFP
                     VIVGGNHTVSIGAIKAFSCRYDNLSVLQLDAHSDLRDEYEGSRYNHACVMARAREYAS
                     LVQTGIRSMSAGERKIADMDRIFFAHDLYYDKSLYSRALDKLTENVYITVDLDVFDPS
                     LIPSTGTPEPGGPGYFEIMHFLRDVVSCRNVIGFDVVELCPSSSNKAPDFVAARIIYQ
                     LLSYRFSK"
     gene            complement(3959..4768)
                     /gene="mazG"
                     /locus_tag="PK288_10025"
     CDS             complement(3959..4768)
                     /gene="mazG"
                     /locus_tag="PK288_10025"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPM19304.1"
                     /translation="MTADREKACEEFRRLLDIMDELREKCPWDKKQTNESLRKLTIEE
                     TYELADAILSGNDEGIRKELGDLMLHIVFYAKIGRERNLFSMADVLNGINQKLIYRHP
                     HVFSDVTVSDSAEVEKNWEHLKIKEDELQKPVLSGVPSSLPAIIKANRIQEKVRGVGF
                     DWEKREQIWDKILEELTELKKEIDNRSTAGIESELGDLLFSIINASRLYDIDPEAALE
                     KTNRKFIKRFNYLEKKTIAKGISLHGMSLDKMNEIWEEAKKNDQEDHLENL"
     gene            complement(4778..5572)
                     /locus_tag="PK288_10030"
     CDS             complement(4778..5572)
                     /locus_tag="PK288_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPM19305.1"
                     /translation="MPVITLTTEWRPDDIYNGIIKGKLCSLCPGTPIIDNAGSIQPFN
                     ISHAAFIVRNTFLNYPSGSIHILCVHSEAHRNEEYLAVKSKGHYFIGTDNGIFYLILN
                     SEPDEVVRIDRGGANDELDLFARTAAGICAGKKLSQLGSSPGQISERLPIRATIDRDN
                     IIGSIIFIDSYGNAISNITREVFYRVFAGKEFRILVQSNKNFTEHISETYNDVPAGEL
                     LARFNILDLLEIAINGDNVSELLSLSTGSTIRIDNINKPAQPNQLL"
     gene            5678..6646
                     /locus_tag="PK288_10035"
     CDS             5678..6646
                     /locus_tag="PK288_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPM19306.1"
                     /translation="MTEVVIFLEDIDPVVFYGANNALLDKIRGFFPKLKIIARGNELK
                     CVGEETEITLFAERFNELVEYYRKYHRLEEHDLEKYLFDRDHMKSAATGEFENVIVFG
                     TSGRPIRARTVNQLLLVNDYRTNDLIIAEGPAGTGKTYTSIALAVRALRDREVKKIIL
                     ARPAVEAGERLGFLPGDMKEKLDPYLQPLYDALHDMIPFKKLETWLEDGTIQIAPLAF
                     MRGRTLENAFVILDEAQNATISQLKMFLTRMGINSKFIMTGDTTQIDLPKKNESGLLQ
                     AMRILANIEGISVIRFDERDIVRHRLVKKIVKAYENEENGDKNSNS"
     gene            6735..>7145
                     /locus_tag="PK288_10040"
     CDS             6735..>7145
                     /locus_tag="PK288_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPM19307.1"
                     /translation="MTQTITDSDFKFLGQTGKYRGKVRDVYNICDKYLLMVVSDRISA
                     FDVVLPRGIPYKGQVLNQIAGKFLDATADIVPNWKIATPDPAVTFGHKCRPYPVEMIV
                     RAHLTGSSWRLYKAGTREICGIRLPDGMREHQRFP"
BASE COUNT         2024 a         1469 c         1726 g         1926 t
ORIGIN      
        1 aaaagcagaa ggaaatgaac aattaccgga gttccctgaa gaagggagac aaggttgtta
       61 caaccggtgg catctatggc agggtttatg aagttaagga taattatgtc atgcttgaag
      121 tcggaggaga tatgaaactc aaggtagata agaatgcctt gctcaaggat cccaccgcca
      181 acgattaaaa aattcattta tctgctgcgt gtacggcaaa atattgatat ggctccgaaa
      241 caaggcaggc agggggatta gtaacataaa cagggattat accatttttg gtttttttct
      301 cctgctgtct tttattttct ggtatcttaa ttcactcggt aaggaaatag aaacggaaat
      361 aagatatccc gtacgttatg taaacctgcc gaaagaaagg acccttgctg aggaacttcc
      421 gtcgcggctc aatctatacc tcaagggtcc ggggtacgcg gttctcaaac ttaaattaac
      481 aggcagcagg gctcccgtca tactggatat ttcagcgctt aattacagga gggtgccggg
      541 aagcagggac cttagttatt acctgaagac ctcgggcatc attccgaagc tcaaaaatga
      601 tcttcgctcg gaatgccaga tcctttatat caagccggat acactgttct tcagattcga
      661 caggatagta agcaaaaagg tgcctgtcgt atcggctgtt gaagtgatta ctgaccggca
      721 atactttata aagggcggta ttgcaattga accggaaacc ataaaaattt cagggccgtc
      781 aggcattctg gataccataa ccgaagtacg gacaaaatac aggaagttca ccagggtgaa
      841 cgaaacctta aaaaaaagta tcagccttga tctgcctgat tattactcgg catctgttaa
      901 gaaagtatca gtaaccattc cggtcgagca gttcacggaa gcagaactga cggttccggt
      961 aaaaatactg aatcagcctg attccattga cctcaagatc tttccggatg ttgttaccgt
     1021 aaagggactt gttgcggtga atgatttcaa gaagctggct gaaataccat ttgaggtgat
     1081 tcttgatatg gacaaggtca atctgaggtc atctgaacgg attcccctgg agatacgcaa
     1141 tgtgccccct tttctgacct cactgaggat cactcctgga gatgtggatt ttcttataga
     1201 gaaaaacaaa aaatgaaaaa gccggtaaat accgtcttca ggctaggagt cacgggaggc
     1261 ataggaagcg gcaaatctac cgtatgcaaa gtgttctgcg cactgggtgt tccggttttt
     1321 tatgctgatc atgaagccag ggtgattatg gataatgata cggacataat ggggaaaata
     1381 aattcggcag ttggccggga catgtacagg aacggttcgc ttgaccgtgt tgaactggcg
     1441 aagctgatat tcaacgacga gatccttctt ggcaagataa actcaattgt gcacccggca
     1501 gtttttgaca gattcgctga ctggtatgcc ggacaggcag caccctacgt aattatggaa
     1561 gctgcaatcc tgtttgaaag cggagcgtcg gaacttgttg acaaagtact tacggttgtc
     1621 actccggttg aagaaagaat agaaagaatg atcaggggaa agaagtttac caggcagcag
     1681 gtcattgagc ggattaataa ccagatggac gatgaaacaa gaatcaggaa ttcagatttt
     1741 cttatttata attctgataa tgacatgatt gtgcccgccg tccttaaagt gcataatgaa
     1801 atcctgcaaa tggtgaaaca ataaaaagtt atgggcagat ttgcaaaatg gataggagga
     1861 ggacttggtt tcgttatggg cggccccatc ggtgcacttc tcgggtttct ggtcggttcg
     1921 atggtagaca gcactgtatc aggccaggtg acttacacgc agccgcagcg tcaaacggca
     1981 aggggtgatt tcagtgtcag cctcctggtc cttatcgcag cgatgatgaa agccgacgga
     2041 aaagtggtga aagccgaact tgactttgtc aagcagtttt ttctaaggca attcggacct
     2101 gaatcggcac gtgaggcact aaggatgctg agagatatac tgaaacagga tatccctgtg
     2161 agggatgttt gcttgcagat aaacaggaac atggattatt catcgagatt gcagcttatt
     2221 cacctgctgt tcaatatatc agtttcagat gcagcagtcc accccgtcga acttgaagca
     2281 attgaaagaa tgtcggggta cctgggcatc agcggctcgg acttcaaatc aataaaaaac
     2341 atgttcgtgc ctgaaaccga ctcggcttac aagatactgg aaattgatcc tggcgcaacg
     2401 gatgacgaga ttaaaaaagc tttcaggaaa atggcaatga aatatcatcc cgataaagtg
     2461 agtcatctgg gagaagaatt taaaaaggtt gctgaagaaa aatttaaaaa ggtcaatgaa
     2521 gcatacgaaa gaataaaaaa ggagcggaat atcatttaat attgataaat ataaaaggaa
     2581 tcattttttg aactggattt atttcaccca caaaacaaca gctatatggt tttaaaggat
     2641 attgtagcga tctctggtca gcagggactt ttcaggttta ttgcccaggg aaagaattcg
     2701 atgatcgtgg agcatcttga aacaggcaaa agaagcagtg ctttcagttc agcccgtgtc
     2761 agctcccttg aggaaatatc cgttttcact gacagtgaag atatgcctct gggaaaggtg
     2821 tttgacagga tttacgaaaa ggagaatggt ggtgaggcaa tagatcccaa atcagatcct
     2881 gaaaccctga gggcatactt cagggaaatt gtgcctgatt attcgaagga taaagtctac
     2941 aacagcgata tcaggaaagt tataatgtgg tataatatcc tgcagcgtaa aaacctgctt
     3001 attcccgaag agcctgaacc agcttccggt gagggtgctg atgcagccgc tgctgaagaa
     3061 aaagctgccg gggaaggaaa ttcagccggg gaagccggga acaagaagga aaaagatggg
     3121 gaatgagccg tagttgcccg aatttcggag gcgaagaggt tgtttatgat tattccggat
     3181 cagggattat cattgtacct gtcccgtatg ataagacaag cacctggatg agaggtgcgg
     3241 ataagggacc cgctgccata atggaggctt cggcgaatct cgagttctat gatgccgaga
     3301 cttcctcgga ggtgcatctt ttgggaattc atacggttga cccggtaacg gaggaagaaa
     3361 ctcctgaaaa acttgttgat gctgttttca atcaggtatt atccattatt gacgacggaa
     3421 agttccccgt aattgtcgga ggaaaccata ctgtaagtat tggtgcgata aaagcctttt
     3481 catgcaggta tgataacttg tcggtactac agcttgacgc tcattctgat ctgcgggatg
     3541 aatatgaagg atcgcggtac aatcacgcgt gtgtaatggc ccgggccagg gaatatgcct
     3601 ccctggtcca gacaggcatc cggagcatgt cagccgggga aagaaagatt gccgacatgg
     3661 accggatatt ttttgcccat gatctttatt atgacaagag cctgtattcc agggcactgg
     3721 acaaacttac agaaaatgta tatataacgg tcgatcttga cgtctttgat ccttccctta
     3781 tcccgtcgac aggtacccct gaaccgggcg gccccggtta ttttgagata atgcatttcc
     3841 tgcgcgatgt agtgtcatgc aggaatgtta tcggtttcga tgtcgttgaa ctttgccctt
     3901 catcctcaaa caaagctcct gattttgttg cagccaggat aatttaccag cttctgagtt
     3961 acaggttttc caaatgatct tcctgatcat tctttttagc ttcttcccag atctcattca
     4021 tcttgtcaag gctcatgcca tgcagggaaa tcccttttgc aattgttttc ttttccagat
     4081 aattgaagcg ttttatgaat ttcctgtttg tcttttccag ggcagcctcc ggatcaatgt
     4141 cgtaaagtct tgatgcattg atgattgaaa acaggaggtc gccaagttct gattcaatgc
     4201 ctgcagtgga acgattgtcg atttcttttt tgagttcagt cagctcctcc agtatcttat
     4261 cccatatctg ttcccgcttt tcccagtcaa atccaacgcc tctcaccttt tcctggatcc
     4321 tgttggcttt tattatggca ggaagggaag agggaactcc cgacagtacc ggtttctgca
     4381 attcatcctc ttttatcttg agatgttccc agttcttttc gacctcggca gaatcagaaa
     4441 cggttacatc tgagaaaaca tgcggatgcc tgtatataag tttctgattg ataccgttca
     4501 gcacatcggc catcgaaaaa aggtttcttt cgcgaccaat tttcgcatag aatactatgt
     4561 ggagcataag atcacccagc tctttcctga taccctcatc gtttccggat aatattgcat
     4621 ctgcaagctc gtaggtttcc tctatggtca gctttctcag gctctcattg gtttgttttt
     4681 tatcccacgg acatttttcc ctcaattcgt ccatgatatc aagcagtctc ctgaactcct
     4741 cacatgcttt ttccctgtct gcagtcatat ttatctttta aagcagctga ttgggctgcg
     4801 ccggcttgtt aatattgtcg attcttatgg tggaacctgt actgaggctc agcagttctg
     4861 aaacattgtc gccgtttatt gcaatttcaa gaaggtcgag tatattgaat ctggcaagta
     4921 attcacctgc aggaacgtcg ttgtacgttt cagatatatg ctcagtgaag tttttgttgc
     4981 tttgtaccag gatcctgaat tctttacctg caaaaacacg gtagaacact tcacgtgtta
     5041 tatttgatat tgcattaccg tacgaatcga tgaaaataat gcttccgata atgttatccc
     5101 tgtcaattgt tgcccttatg ggcagccttt cacttatctg gccgggggag cttccaagtt
     5161 gtgaaagttt ttttcccgca cagatgcccg ctgccgttct tgcaaaaaga tccagttcat
     5221 catttgctcc gccccggtcg atcctgacaa cttcatccgg ttcggaattc agtatcagat
     5281 aaaaaatccc gttgtcggtg cctatgaaat aatgaccttt tgatttcact gcaagatact
     5341 cctcattcct gtgtgcttca gaatgcacac ataaaatatg gatcgatccc gacggatagt
     5401 tcagaaaagt atttcttaca ataaatgccg catgggaaat attgaaaggc tgaattgaac
     5461 cggcattgtc gattattggt gtgccaggac acaaactgca tagtttgcct ttaatgatac
     5521 cattgtaaat atcatcgggt ctccattcgg ttgttaatgt tattaccggc atctggaaaa
     5581 aacttacgac aaagataatc ttataaagaa aaaataagct tactttgttg cttcagaaga
     5641 tcatttttcc ggttgcgggc tagcatctta actattaatg acagaagttg taatatttct
     5701 cgaagatatt gatcctgtgg ttttttatgg tgcaaacaat gcacttctcg acaaaatccg
     5761 gggttttttc ccaaaactta aaatcattgc aaggggcaat gagctaaaat gcgtgggaga
     5821 ggagacggaa ataactttgt tcgcagaaag attcaatgag ctggttgaat attacaggaa
     5881 gtatcacagg cttgaggagc atgatcttga gaaatatttg tttgacaggg atcatatgaa
     5941 gtcggctgcc accggtgaat ttgagaatgt gatcgttttt ggtacaagcg gcaggccgat
     6001 aagggccaga acagtaaacc agctgctcct ggtgaatgat taccggacaa acgacctgat
     6061 cattgcagaa ggtcctgccg ggaccggaaa gacttatacg tcaattgccc tggctgtgag
     6121 ggcgctacgg gacagggaag tcaagaagat cattcttgcc cgtcctgccg ttgaagcagg
     6181 ggagaggctg ggttttcttc cgggcgatat gaaggaaaag cttgaccctt accttcagcc
     6241 gctttatgac gcacttcatg acatgatacc attcaagaag cttgagacat ggcttgagga
     6301 cggtaccatc cagattgcac cactggcatt tatgaggggc cggacccttg aaaatgcctt
     6361 tgtcatactc gacgaggccc agaatgcaac aataagtcag cttaagatgt ttcttaccag
     6421 gatgggaata aactccaaat tcataatgac cggagacacg acgcagatag atctgccaaa
     6481 gaaaaatgag tcaggactcc ttcaggccat gagaatactt gcaaacatcg aggggatatc
     6541 ggttatcagg tttgatgaaa gggatattgt aaggcaccgg ctggtgaaga aaatagtaaa
     6601 ggcctatgag aatgaggaaa acggagataa aaacagtaac agctgatcct ttttcaggcc
     6661 ggaggcatga aggatacatc ttctcatgct cgcccgcctg ttgcggagtg ctgtcgggca
     6721 gattttagca gattatgacc cagaccatta cagactcaga ctttaaattc ctggggcaga
     6781 caggcaagta ccggggaaaa gtaagggatg tgtacaatat ttgtgacaaa tatctgctta
     6841 tggtagtgtc agacaggatc tctgcctttg atgtggttct tccaagaggg atcccataca
     6901 agggtcaggt gttgaaccag attgcgggaa aattcctgga tgccacggcc gatattgttc
     6961 ccaactggaa aattgcaaca ccagacccgg cagtcacatt cggccacaaa tgcaggccct
     7021 atcctgtgga aatgattgtc agggcgcatc tcacaggcag ttcatggcgc ctgtacaaag
     7081 ccggcaccag ggaaatatgt gggatcaggc tccctgacgg aatgagggaa catcagaggt
     7141 ttcct
//
LOCUS       DAOOYG010000139         7080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_12067_length_7080_cov_5.766406, whole genome shotgun sequence.
ACCESSION   DAOOYG010000139 DAOOYG010000000
VERSION     DAOOYG010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12067_length_7080_cov_5.766406"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..756
                     /locus_tag="PK288_10045"
     CDS             253..756
                     /locus_tag="PK288_10045"
                     /inference="COORDINATES: protein motif:HMM:NF027625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4943 family protein"
                     /protein_id="HPM19308.1"
                     /translation="MKVPLISFILVTAMGFSGCEKYNSSRDVDKYIDELISGTYKSYD
                     LPDFNTTDISALLEYRNETTVITNFPQNPVSSFRQSECKLGMMVLWTVESLRAVENNS
                     RFLIGRFPSLNPVLVLKESSEPQMVFDDKSHKEAAKAYYDWWNSCRPFKDKISTDPLA
                     KTHYRWH"
     gene            747..1304
                     /locus_tag="PK288_10050"
     CDS             747..1304
                     /locus_tag="PK288_10050"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HPM19309.1"
                     /translation="MALVLKNRPVHTRLAYVLRQEFPVREDCQAAGSAPRPCSYRHWN
                     KFIAELNGKNPCRRSQINMNIFLKFKTTINMHKSIVIFLLLIHCGMISGQKVYSVGYP
                     NQADVKVFVVKYENQADLKVFRVKYENQAGENNGKWFFTEYPNQAGKKIYFVEYQNQA
                     DLKIYFVKYENQAGWREKNKMHLMY"
     gene            complement(1578..3566)
                     /locus_tag="PK288_10055"
     CDS             complement(1578..3566)
                     /locus_tag="PK288_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPM19310.1"
                     /translation="MKKLLKYLLRTVGALIGAVILLLAVGFLVIKISTVRAEKKARAL
                     GGPEVKTITTEGYTFRDLNKNGKLDVYEDSRKPVNERVEDLLSQMNLEEKAGTMFFSM
                     IGMNGDGSPAESPSLSNPFSFMFMGTTKMILQKKINHFNILLGTGKKEMAAWNNSLQK
                     LAERTRLGIPVTIGTDPRNHFSNNPLASAFAGDFSQFPEPIGLAAIGDSLTVAEFADI
                     ARREYLAVGIRVALHPQVDLATEPRWGRINSTFGEDAALSSRLAYAYIKGFQGDTMGP
                     CSVACMTKHFPGGGPQKDGIDPHFKVQKGQVYPGNNFNYHLLPFEAAFRAGTAEIMPY
                     YGVPRGIGMEEVGMSFNKEIITGLLREKYKFDGIVCTDWGIISDWKVFGRTIMTARCW
                     GMESYSGEDRIVKALNAGVDQFGGETVPELIVRAVKAGKLSESRLDTSVRRLIRMKFI
                     QGLFDDPYVDENKAVEIVGNPAFRAAGELAQRSSVVLLKNDTIDKKAVLPLKKGTRIY
                     IENIDPEVAAEYGQVVKKPSEADFAILRIKAPSGVLKGAGILGRLFSSGDLDFREKEK
                     SKILRLIDKVPSIVDIYTDRPAVIPEIAAASRGLLANYGCSDAAILDVVFGVFNPVGK
                     LPVEMPSSMDAVRKQKEDVPFDSENPAFRFGYGLSYHN"
     gene            complement(3602..5791)
                     /locus_tag="PK288_10060"
     CDS             complement(3602..5791)
                     /locus_tag="PK288_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPM19311.1"
                     /translation="MKKLSRVLLYILAGALFLFVILVVFFLLKYNSLEKKVISTRDAL
                     EKTENRIVLVSGRQANGSGLNLLPVPKKVTEGKGRFKMPGKLSFTVADSLEGNVRYWL
                     DRIDGVRSVSTHAGGTVIFRFRPGIHRQGYKMEITPARIRVEYSSAEGLFYSIISLKV
                     LNRNYEGNIPCITIEDWPDLEVRGLMFDISRNKIPTRETLFRLAETLADLKFNHLELY
                     IEGFSFAYPSFTDLWAETETPVTGEDIKALDTLCRKNFIDLVPNQNMFGHMMSWLNTE
                     QYADLAECPEGFRLFGLIDMKGTLDPSDPRSIELVTRMTDDLLPNFTSGYFNVNMDET
                     FDLGKGKSKKLCEEKGVVNVYMDYALKVHDIVTSRGKKMMMWGDIVLRSPEALNRIPP
                     DVTLLDWGYEAMYPFEKNAGNFRAAGVNFMVCPGTSSWSSLVGRSDNMKANISNAVDA
                     GVKHGAKGMLLTDWGDMGHWQYLPVSYPGYCLGGALAWNNVGADNVPLETFLSSYMYR
                     DKSGIMGQLAFNLGKYPAYEEKPGVSMTSTVMGFQLGIRDEVLVEEIWRKMQEGITVL
                     MGDLSPELISGFMESYNKRHAYNFEGMYALIDSANILLEKTSPGGDDGLLVRNEYLNT
                     LKLLKTAVDLQYYIQNRRLLSMEKQKELLEGMDKTLTGYIETNKILWLERNKPGGYET
                     STSSLYSLKKQIIARLKGLEKSSFGRTVDRFFGKIVTAIAVTYIRNS"
     gene            complement(6133..6267)
                     /locus_tag="PK288_10065"
     CDS             complement(6133..6267)
                     /locus_tag="PK288_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19312.1"
                     /translation="MKKSLFTILGIMILGLHGLAQDFDRAKPDQYFDALEANNRFRDT
                     "
     gene            complement(6392..6835)
                     /locus_tag="PK288_10070"
     CDS             complement(6392..6835)
                     /locus_tag="PK288_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19313.1"
                     /translation="MTERKNSSFCATPQIIYADYHGTWSMVNDTLVQISVGYWGGLAR
                     YRLDIEHVDKNSLTVRPIVEDNVWVFINETQCANAWDNIIAHDTETRVKKYLQSNDIS
                     VFDIKIETYSNGPFCEACFCPSGRRIKVLIRESDLEPAIKLGFKK"
BASE COUNT         1831 a         1731 c         1511 g         2007 t
ORIGIN      
        1 ttttgtcttc atcttaaact gtttttaatg atttgtgtct tttcgtcgga gcaaaagtat
       61 aagcagcctc ccgggacgaa aaataaaaga tgacaagggc gtgaaaacag gtgctgaaag
      121 tgcaatcagg aggaatgaag gtgaccgggc ttatgcgtcc ccggccggct tgctgatact
      181 gtttcatccc ggaaatcaat aaaccatatg gtacagtttt tgtaccaacc agattaatac
      241 gacattaacc cgatgaaggt tcctctcata tcattcattc tcgttaccgc aatgggtttt
      301 tctggttgtg aaaaatataa cagcagtcgt gacgtagata aatatattga tgaattgatt
      361 tcgggcacat ataaatcata tgatctgccg gatttcaata ccactgacat atccgcctta
      421 cttgagtaca ggaatgaaac cactgtcatc acaaactttc cccaaaaccc ggtttcttct
      481 ttccggcaat cagaatgtaa actggggatg atggttttat ggacagtaga atccctaagg
      541 gctgttgaaa acaacagcag gttcctaata ggaaggtttc cttcactaaa ccctgttctt
      601 gtgctaaagg agtcttcaga accgcagatg gtttttgatg ataaatcaca taaagaggct
      661 gccaaagcct attacgactg gtggaattca tgccgccctt ttaaggataa gatcagcact
      721 gacccgctgg cgaaaacaca ttatagatgg cattagtctt gaaaaacaga ccggttcata
      781 ccaggctcgc ttatgtgctg cgtcaggaat ttccggtaag ggaggactgt caggcagcag
      841 gcagtgcgcc ccggccctgt tcataccggc actggaacaa attcatagca gaacttaacg
      901 gcaaaaatcc ctgccggcga agccagatta acatgaacat attcctgaaa ttcaaaacaa
      961 caattaatat gcacaaatcc attgtgattt ttttactgct catccactgc ggtatgatct
     1021 ccggacaaaa agtatatagt gtcggatatc ccaaccaggc agatgtgaaa gtatttgttg
     1081 taaaatatga aaaccaggca gacctgaaag tattcagggt gaaatacgaa aaccaggctg
     1141 gtgagaacaa cggaaagtgg tttttcactg aatacccgaa tcaggcaggg aaaaagattt
     1201 attttgttga ataccaaaac caggcagact tgaagattta tttcgtcaag tatgaaaatc
     1261 aggccgggtg gagggagaag aacaaaatgc atctgatgta ttgaacagat ctgatcttct
     1321 tttcagaaag cctttcccgt cagcgcaaag gcaatggcgg caatccaaaa aaggtaaagc
     1381 aggttccctt actgacaatg ccatgaatgt cagctgttaa acgggtccgg gaacggagct
     1441 tattgactat cgtaccatac atcagacaga tatagtacga cattaatata ttatcaataa
     1501 taaataattt atattgaaaa ttatacgaac cctatcttaa ttaattttcc cgatccaggt
     1561 tttactgccg gaccggctca gttatggtac gacagaccgt aaccaaacct gaaggccgga
     1621 ttctccgagt cgaaaggcac atcctctttt tgctttctga ctgcatccat cgatgatggc
     1681 atttctacag gaagttttcc cacggggtta aataccccaa atacaacatc aagaattgct
     1741 gcatcgctgc atccgtaatt tgccaggagg cccctgcttg cagcagctat ctcaggtatt
     1801 acagcgggcc tgtcggtgta gatatcaact attgacggaa ccttatcaat gagacggagt
     1861 atttttgatt tctccttttc cctgaaatca agatcgccgc tggaaaaaag acgcccaagg
     1921 attcctgctc ctttcagtac acccgacggc gctttgatcc ttaatatcgc aaaatctgct
     1981 tcagaaggtt tttttacaac ctgtccgtat tcagctgcca cttcaggatc tatattctca
     2041 atatagatcc tggtcccctt tttcagcgga agaacggcct ttttatctat tgtgtcattt
     2101 tttaaaagta ccactgagct tctctgtgcc agttcgccgg ctgccctgaa tgccggattg
     2161 cctacaatct ccacggcttt gttctcatcc acgtaaggat catcaaacaa accctgtatg
     2221 aatttcattc ttatcagcct ccttacagaa gtgtcaagcc tgctttctga aagttttccg
     2281 gccttcaccg ccctgacaat aagttctggg acagtttcac ctccgaattg gtccacaccg
     2341 gcattcaggg ctttcacaat cctgtcctcc ccggaataac tttccattcc ccagcacctt
     2401 gcagtcataa ttgttctccc aaaaaccttc cagtcggata tgatacccca gtcagtgcat
     2461 acaatgccat caaatttgta cttctcacgg agaagaccag tgataatttc cttgttaaac
     2521 gacatcccca cctcttccat tccgattccc ctgggaaccc cgtaataagg cattatctcc
     2581 gccgtccctg ccctgaaagc agcctcgaaa ggcaggaggt gataattgaa attattcccg
     2641 gggtatacct gccccttctg aaccttgaaa tgcggatcta taccatcttt ctgcggaccg
     2701 ccgccgggaa aatgttttgt catgcaggca acactgcatg gtcccattgt atctccctgg
     2761 aagcccttga tatacgcata tgcaagcctg gaggagagtg cggcatcctc cccgaatgtc
     2821 gagtttattc ttccccaccg gggttctgtc gcaaggtcga cctggggatg tagtgctacc
     2881 ctgatcccaa cggccaggta ttccctgcgc gctatatctg caaattcagc aacagtcagc
     2941 gaatctccta tggctgcgag tccaatcggt tcagggaact gggaaaaatc acctgcaaat
     3001 gctgatgcaa ggggattatt gctgaaatga ttacgcggat cagttccaat tgtaacaggt
     3061 ataccaagtc tcgtcctttc tgcaagcttc tgtaaactgt tattccacgc agccatctcc
     3121 tttttcccgg tcccaagaag gatattgaag tgatttattt tcttctgaag gatcattttt
     3181 gttgtcccca tgaacatgaa ggagaagggg ttggaaagcg acgggctttc tgccggcgat
     3241 ccgtcaccgt tcataccgat catcgagaaa aacattgttc ccgccttctc ctccaggttc
     3301 atctgcgaga ggaggtcctc aaccctttca ttgaccggtt tccttgaatc ttcatagaca
     3361 tccagcttcc cgttcttgtt aaggtcgcgg aaagtatatc cttcagttgt gatggttttt
     3421 acttcaggtc ctccgagggc cctggctttc ttttctgcac gaacggttga gattttaatg
     3481 acaaggaagc caacagcaag caggaggata acggccccaa tgagcgcacc gactgtccgg
     3541 agcaggtatt tcagcagttt tttcatcttt atcgttttaa gggagatcgc ggaataataa
     3601 gctaagagtt ccttatgtaa gtgacagcaa tggcagtaac tattttcccg aaaaacctgt
     3661 cgactgtcct gccgaacgag gatttttcaa gacccttcag cctggctatt atttgttttt
     3721 ttaatgaata cagggaggaa gtacttgttt cataaccccc cggtttattt ctttcgagcc
     3781 agagtatttt attggtttcg atatatcccg ttaatgtttt atccattcct tccaggagct
     3841 ccttttgctt ttccattgaa agcagcctcc tgttctggat ataatactgg aggtcgacag
     3901 ctgttttaag aagcttcagt gtattgaggt attcatttct taccagcagg ccgtcatctc
     3961 ccccggggct cgttttttca agaaggatat tggcagaatc aatcagtgca tacatccctt
     4021 caaaattgta agcgtgcctt ttattatagg attccatgaa accgcttatc agttccgggg
     4081 aaaggtctcc catgagaacc gtgattcctt cctgcatttt tctccatatc tcctccacca
     4141 ggacttcatc cctgatcccg agctggaaac ccatgacagt gctggtcatc gatacgccgg
     4201 gtttctcctc atacgccggg tatttcccca ggttaaacgc cagctggccc atgattcctg
     4261 atttgtcgcg atacatgtac gaggaaagga atgtttcaag gggcacattg tcagcgccga
     4321 cattattcca cgccagtgcc cctcccaggc aatagcccgg gtagctgacg ggcaggtact
     4381 gccagtgacc catgtcgccc cagtcggtca gcagcattcc ttttgccccg tgtttaacac
     4441 cggcatcaac cgcattgctg atattggctt tcatgttgtc actgcggccc acgagacttg
     4501 accagctgct cgtcccggga caaaccatga aattaacccc ggcggccctg aagtttcctg
     4561 cattcttttc aaaaggatac atggcttcat atccccagtc gagaagcgta acatcagggg
     4621 gaattctgtt cagcgcttca ggcgagcgca gcactatgtc tccccacatc atcatcttct
     4681 tacccctcga tgttacaata tcgtggactt tcaacgcgta atccatataa acattaacca
     4741 cgcctttttc ctcgcacagc tttttgcttt taccttttcc caggtcgaac gtctcatcca
     4801 tgtttacatt gaaatatccg gaggtgaaat ttggcagcag atcgtcggtc attctcgtta
     4861 caagctctat gcttcgcgga tcggacgggt cgagggttcc tttcatatcg atcaggccaa
     4921 acagcctgaa gccttccgga cattcagcca gatcggcata ctgttcagtg ttgagccacg
     4981 acatcatgtg cccgaacatg ttctggttgg gaacaaggtc tatgaagttt ttcctgcaaa
     5041 gggtatcgag ggcttttatg tcctcacctg ttaccggggt ttcggtttct gcccagaggt
     5101 cggtgaatga agggtaggca aaggagaatc cttctatata aagttcaaga tgattgaatt
     5161 taagatcggc cagtgtctcg gcaagcctga agagggtctc ccttgtcggg atcttgttac
     5221 ggcttatgtc gaacatgaga cctcgcacct ccaggtcagg ccagtcttct atggtaatgc
     5281 atgggatatt tccttcataa ttcctgttaa gcaccttcaa acttatgatt gagtagaaaa
     5341 gaccttcagc cgaactgtat tcaaccctta tcctggcggg agttatttcc atcttgtaac
     5401 cctgcctgtg tatgccgggc ctgaacctga aaatgactgt ccccccggca tgggtactga
     5461 cagaccttac cccatcaatc ctgtcaagcc agtacctgac attgccttca agagaatctg
     5521 caacggtgaa ggataatttc ccgggcattt taaaacgtcc ttttccctcc gttacctttt
     5581 tcggaacggg gagcaggtta agccccgaac cgtttgcctg ccggccggat accaagacga
     5641 tcctgttttc agtcttttca agtgcatccc ttgtgctgat tactttcttt tcgagggagt
     5701 tatacttaag taaaaagaaa acaaccagga taacaaagag aaataaagca ccggctaaaa
     5761 tataaaggag gacccgtgac agttttttca tggttacctg ttttaagcta acctgttgtc
     5821 aggtaacagt aaagttaaat tgtttttccg acaatctgtt tgagaatcta taataacggc
     5881 agggaaaaat gcgaataccg gactgaaccc gtctataccc cctgtatgcc cgtaaccggt
     5941 cctgtcataa aaggggaatt ggaacaatcc cattccaggc tggtccctga tagttttcat
     6001 catttcaagg ctctggctgc cagggagcct gccgccaaac agggcatcgc cgaatcttgt
     6061 aatgtccgcg gccgtggata caatgcctcc agcgtccaat ggaatggaga tatctgtttc
     6121 cgattcagct tttcaggtat cccggaacct gttatttgct tcaagggcat caaaatactg
     6181 atccggcttt gccctgtcaa agtcctgtgc cagcccgtga agtcccagga tcattattcc
     6241 caaaatggta aacaacgact ttttcatttt cccggtgatt tttatcacgt aaagttaagc
     6301 tgtttaaatc atcttttaac ggtgattact attcttttac agagctttta cactcatgtc
     6361 agtcgctgtg caccgataga atttattggg attatttctt aaatcccagt tttatagccg
     6421 gctcgaggtc cgattcgcgg atcaggacct tgatccgtcg tcccgatgga cagaaacagg
     6481 cttcgcagaa tggcccgttg gaataggttt cgatttttat gtcaaataca ctgatgtcat
     6541 tactttgcag gtattttttt accctggttt ccgtgtcgtg cgcaatgata ttgtcccacg
     6601 cgtttgcaca ctgggtctcg ttaatgaaca cccatacatt gtcttcaacg atcggcctta
     6661 cagtgagaga atttttatct acatgttcta tatcaagcct atacctcgca agtcctcccc
     6721 agtatccaac cgagatctga accagggtat cgtttaccat cgaccaggtg ccgtgataat
     6781 ctgcatatat tatctgcggc gtagcacaaa agcttgaatt ctttctttcg gtcatggtac
     6841 cgtcactgtt aaatttataa cagtgattat caataaaatg atcgcttctt ttaaatactg
     6901 atgaattacc ctggtattca gagtagttcc agatccctat cagcaggttg ccgggatcga
     6961 tccgggtatc atccttcttc caatgatttc acggcattgt cattgcttag aataaatgac
     7021 ttgacgagcg gtttgtcaag tataccggaa agaccatcaa tatgcctggc atctgtcctt
//
LOCUS       DAOOYG010000140         6999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_12261_length_6999_cov_4.584533, whole genome shotgun sequence.
ACCESSION   DAOOYG010000140 DAOOYG010000000
VERSION     DAOOYG010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12261_length_6999_cov_4.584533"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PK288_10075"
     CDS             <1..1208
                     /locus_tag="PK288_10075"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPM19314.1"
                     /translation="LIMKNILNIGALLVLIQSVTWLGRDENRDRVSEYFILLISTLTG
                     MNFMISAGHFLMFYIGLELATIPLTALAAYERYSNKSAEAGIKLILISALSSGVLLYG
                     LAMLYGTTGTLYFEEAAHLLKNNNLQTLGFIFFFSGMAFKISIVPFHLWTADVYEGAP
                     VSITSYLSVISKGAAVFIFIIVLFTVFPVIMASWQKAIYATSVLTMTIGNLFAIRQQN
                     LKRFLAFSSISQAGYIFLGIIGGNAAGMASVIYYILVYIFSNLGAFGAVAAISNASGR
                     EDLKDYNGLYRTNPGVSLIMTLALFSLAGIPPVAGFFGKFFLFAAAAEKGYYLLVTIA
                     VLNTIISLYYYLLVVKAMFINANENPVESFKSDIPTKLALGICTAGIFLTGFASPVYE
                     AIRNLSFGI"
     gene            1215..1562
                     /locus_tag="PK288_10080"
     CDS             1215..1562
                     /locus_tag="PK288_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19315.1"
                     /translation="MKPVMGINNAKHVIGEINGVRCTIIETSASGERALFLRQLLEFN
                     NLEVVESVNPPSSEDEEPTYSIGVTDIVFNPVFAIYECLLKIPGGGYVTPGYWLQECT
                     DCDNRYWLRKKDQ"
     gene            complement(1623..2375)
                     /locus_tag="PK288_10085"
     CDS             complement(1623..2375)
                     /locus_tag="PK288_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPM19316.1"
                     /translation="MRKFGLIGFPLGHSFSAGYFDSKFHMENIRDCSYRNYPLTDIEE
                     LPGLIGSDPEIIGLNVTIPYKTEVIRYLDYIDPEAEKVGAVNVLKINRSDGRFHIRGF
                     NSDIYGIRATLMPFAVEELRNALILGTGGSSKAVSHVLSEMHVHFSLVSRTVKPDCLT
                     YRDVQTGLPAGTRLIVNTTPVGMLPDIGQKPDIRYDLLTGDHILFDLIYNPEKTAFLK
                     AGEERGCRILNGLKMLYSQAERSWEIWNDPAF"
     gene            complement(2408..3196)
                     /locus_tag="PK288_10090"
     CDS             complement(2408..3196)
                     /locus_tag="PK288_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HPM19317.1"
                     /translation="MTIMKHELPFIPQRPAKPRKSGITMVMDKGLSIRAAEDFMSVGS
                     EYTDMVKMGFGTSLLTPGFERKLEIYREAGCMPYFGGTLFEAFVIRKMFREYVELLDK
                     YKVELVEVSDGSYEMDHKRKLEYIRILASRGIVISEVGSKKKDVVYSPAEWVLMMKSE
                     LAAGSLKVIAEARESGTIGIYNEDGSVNMALIETISDHVSLENVIWEAPLKSQQAWFI
                     RHFGANVNLGNIAPEEIIPLESLRRGLRGDTFFQFLPDDIDESK"
     gene            complement(3284..4693)
                     /locus_tag="PK288_10095"
     CDS             complement(3284..4693)
                     /locus_tag="PK288_10095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPM19318.1"
                     /translation="MEEDRSGFSFEEEIRSAVQKFERMRSNNQSCFFDVIEFESIIDY
                     YIESNNPLKAYEAAILAAEQHPNSVSIQLRKARVLLDRGRAVEALRILKNLESIEPGN
                     HEICVAKGTALGMLGDIQGAKKMFDYALTLDSDDTANILFSITSVLQNLNYYDYLIPY
                     LKKLIQLEPDFYAHLYDLAYAFEKTENYAESIRYYRKYLEEEPFSDSAWYNLGIIYNK
                     LDEPEKALTAYDYSLAINSSNTFALFNKGNILYNQEKYVEAVPVYHEYLENEPDNFEA
                     MTYLAECYEKTGDLVLARKYYNEAIELAPDFADPWFGLGILALDEGNPSESLVFLRKA
                     VRLDDENPEIWYILGKAHYASGEEKAALRCFREALKIDVYYNEVWSDLGRIILNEGYI
                     KRALPYLEKAYKIIGDIPGINYLLGAFHCHLGNTEKAFRHISLALSSEKEAFYEFRDI
                     FPVKIHNRKIKRLFEEYNI"
     gene            complement(5065..5940)
                     /locus_tag="PK288_10100"
     CDS             complement(5065..5940)
                     /locus_tag="PK288_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HPM19319.1"
                     /translation="MYTLIDGNKIASEIKSEIAREVARMVSSGIKIPHLAAILVGRNP
                     SSETYVNNKVKDCEEVGFKSTLLRLSEEITENDLLAKIRELNEDDDVDAFIVQLPLPG
                     HISESSVTEAIDPSKDADGFHPVNLGRMVAGLPGYLPATPFGVIELLDRYGINVSGRN
                     CVILGRSNIVGRPLSILLSQKGRDATVTLVHSRTRNIPELVRKADIVIAALGSPGFVK
                     ADMISDGAVVIDVGTTRVESPLSKKGWRLAGDVDFDNVAPKCSYISPVPGGVGPMTRV
                     ALLKNTLLAAMRRVR"
     gene            complement(5949..>6999)
                     /gene="ffh"
                     /locus_tag="PK288_10105"
     CDS             complement(5949..>6999)
                     /gene="ffh"
                     /locus_tag="PK288_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPM19320.1"
                     /translation="KSDINIKINPAVILIAGLQGSGKTTFSGKLAKWVKTKRGKNPIL
                     VAGDVYRPAAIEQLKIIAAQAEVPVYTEDGNMNPVQIVKNSVREAKMKNHDVVIIDTA
                     GRLAVDEEMMNEIASIKNTVSPHEILFVVDSMTGQDAVNTAREFNERLDFNGVVLTKL
                     DGDTRGGAALSIRSVVNKPIKFVSSGEKIDAIDIFYPSRMADRILGMGDIVSFVEKAQ
                     EQYDLEEARKLQKKIARDQFDFNDFIAQIQQIKKMGNVRDLMSMIPGVGKAVKDLDID
                     DNAFKSIEAIIRSMTPGERRNPVILNGSRRKRIATGSGTTVQDVNRLLKQFDETRRMM
                     RMMTKGRNAAGLMRK"
BASE COUNT         1830 a         1691 c         1401 g         2077 t
ORIGIN      
        1 ggctgataat gaaaaacata ctgaacatcg gcgctctgct tgttctcatc cagtcggtga
       61 cctggctagg gagagacgaa aaccgcgaca gggtgagtga gtatttcatc cttctcatat
      121 ccacactcac cggtatgaac ttcatgatct cggcagggca ttttcttatg ttctatatag
      181 gtcttgaact tgccacaata ccgttaaccg ccctcgcagc ttatgaaaga tacagcaata
      241 agtcggccga ggccgggatc aagctcatcc tgatatcggc tctttcgtcg ggagtattgc
      301 tttacggtct ggccatgttg tacggaacaa ccggaacgtt gtattttgaa gaggctgcac
      361 acctgctgaa gaacaacaac cttcagaccc tgggtttcat atttttcttt tcaggtatgg
      421 cctttaaaat ttccatagtt ccattccacc tctggacagc agacgtatat gaaggagctc
      481 ccgtcagtat aacatcgtat ctttccgtaa tttcaaaagg agctgcagta ttcattttca
      541 ttattgtcct cttcaccgtg ttcccggtaa ttatggcctc atggcagaag gcaatttatg
      601 ccacatcagt actaaccatg accataggca atctttttgc cataaggcag cagaacctca
      661 aaagatttct ggcattttca tccatctccc aggccggata catctttctt ggaattatcg
      721 gaggaaacgc tgccggcatg gcatctgtca tttattacat actggtatac attttttcaa
      781 atcttggtgc attcggcgct gtcgcagcaa tctcaaacgc cagcggcaga gaggatctga
      841 aggattacaa cggactttac aggaccaatc cgggagtgag cctgatcatg accctggccc
      901 tgttttccct tgccgggatc ccgcccgtgg ccggcttctt cggaaaattc ttcctcttcg
      961 ccgctgctgc cgagaaaggt tactatctgc tggttacgat tgcggtgctt aatacaatta
     1021 tctcacttta ttattatctt ctggttgtca aggcaatgtt tataaatgca aatgagaacc
     1081 ccgttgaaag ctttaaaagt gatatcccaa ctaaactggc cctggggata tgtacggccg
     1141 gcatattctt aaccggattc gccagtcccg tttatgaagc gataaggaat ttaagctttg
     1201 gcatctgaat aaatatgaaa ccagtcatgg gcataaacaa tgcaaaacat gtaataggag
     1261 aaataaatgg tgtgaggtgc accattatag aaacatcggc tagcggtgaa cgagccttat
     1321 tcctgcgcca gctcctggaa ttcaataacc tggaagtggt tgaatcggtg aatccgccct
     1381 catcggaaga tgaagaaccc acatacagca ttggcgtcac tgatattgtt ttcaatcctg
     1441 tttttgccat ttacgaatgc ctcctcaaaa tacccggagg cggttatgta actccgggct
     1501 actggctcca ggaatgtact gattgcgata acaggtactg gctgaggaaa aaggaccagt
     1561 gacccgtgag gtgttttgaa tttatagaat catgtcagat taaaaataag atccccgatc
     1621 aatcagaatg ccgggtcatt ccatatctcc caggatcttt cagcctggga ataaagcatc
     1681 tttaatccgt taagtattct gcagcccctc tcctctcctg ctttcaggaa tgctgtcttt
     1741 tcaggattgt aaataaggtc gaaaaggatg tggtctccgg tcagaaggtc atacctgata
     1801 tcgggttttt gaccgatgtc aggtaacatg ccaacagggg tggtattgac gatcagcctt
     1861 gttcctgctg gcagtccagt ctgtacatcc cgatacgtga ggcagtccgg ttttacggtt
     1921 cttgaaacaa gtgagaaatg cacatgcatt tctgacagta catggctcac cgccttcgaa
     1981 ctgcccccgg tgccaagaat gagtgcgttc cgaagttcct ctactgcgaa aggcattaag
     2041 gttgccctga tcccgtaaat atcgctatta aaccctctga tatgaaacct gccgtcagac
     2101 ctgtttattt taagtacgtt aactgcccca accttttcgg cttcgggatc aatgtaatcc
     2161 agatacctta tcacctcagt tttgtaagga atagtgacat tcagcccgat gatttccgga
     2221 tctgacccga tcagacccgg cagttcctca atatccgtca ggggataatt tctgtaggaa
     2281 caatcgcgga tattttccat gtggaattta ctgtcaaagt aaccggctga gaatgaatgc
     2341 cccaatggaa agccgatgag gccgaatttt cgcataatta cagaaccgtg ggtatgacta
     2401 agggtttcta ttttgattcg tcgatgtcat ccggcagaaa ctgaaagaaa gtatcaccac
     2461 gcaaccctct tctcagcgat tcaaggggaa ttatttcctc aggtgcaata tttcccagat
     2521 tgacatttgc gccgaaatgc cggatgaacc aggcctgctg tgatttaagg ggagcttccc
     2581 agataacgtt ttcgaggctt acatgatctg atatggtttc aatcaaggcc atattgactg
     2641 atccgtcttc gttataaatg ccaattgttc ctgattcacg ggcctcagca atcaccttaa
     2701 gcgatccggc tgccagttct gatttcatca tcagcaccca ttcagcagga ctgtaaacga
     2761 catctttttt ctttgatccc acttcagaaa tgacaatacc gcgcgatgca agtatccgta
     2821 tatattcaag ctttctctta tgatccattt cataagatcc gtcggatacc tcaacaagct
     2881 caactttata tttgtcgaga agttcaacat attccctgaa cattttcctg attacaaagg
     2941 cttcaaacaa tgttcctccg aaatatggca tacatccggc ttctctataa atttcaagct
     3001 ttctttcaaa tccgggtgtg agcagggatg ttccgaatcc catttttacc atatctgtat
     3061 actcgcttcc taccgacata aaatcctcag ctgcccgaat gctcaatcct ttgtccatta
     3121 ccatggtgat acctgatttt ctgggctttg ccggcctctg gggtataaag ggaagttcat
     3181 gtttcataat agtcattcag gttaatgatt aaataatacc atacaatgtt catcaattaa
     3241 tgtccattgc acagaattat gaagaaacag cagcaaggcg gtatcagata ttatactctt
     3301 caaacagcct ttttatcttt ctgttatgaa ttttcaccgg aaaaatgtcc ctgaattcat
     3361 aaaaagcctc tttctcagac gacaaagcca gggagatatg cctgaaggcc ttttcagtat
     3421 ttcccaaatg gcaatgaaat gccccgagaa gataattaat tcccggtata tcgccgatga
     3481 tcttataggc cttttcaaga tatgggagtg ctcttttaat atatccctca ttcaaaataa
     3541 tcctgccgag atcggaccat acctcgttgt aatatacatc tattttcaaa gcctccctga
     3601 agcagcgcag ggcagctttt tcctctcccg aggcataatg tgccttgccc agaatatacc
     3661 atatttcagg gttttcgtca tcaagcctta ctgcctttct gagaaatacg aggctctctg
     3721 aagggtttcc ttcatcgagg gcaagtattc cgagaccaaa ccagggatcg gcaaaatcag
     3781 gagccagctc tattgcttca ttataatatt tcctggccag caccaggtcg cctgtttttt
     3841 catagcattc agcaagataa gtcattgcct cgaaattgtc cggttcattt tccagatatt
     3901 catgataaac cgggacagcc tcgacatatt tttcctgatt atacaggatg ttacccttgt
     3961 tgaacaatgc aaaggtattc gaggaattta tggcaagtga ataatcataa gcggtcagag
     4021 ctttttcggg ttcatcaagc ttgttgtata ttatccccag attataccat gcactgtcgg
     4081 agaaaggttc ctcctcgagg tactttcggt aatacctgat gctttcagca taattctccg
     4141 ttttttcgaa agcataggcc aggtcatata agtgagcata aaaatcgggt tcaagctgaa
     4201 tgagtttctt caggtatggg ataaggtaat cgtaataatt gagattttgc agaactgaag
     4261 taatggaaaa gagaatattg gcagtatcat ctgaatcgag tgtgagtgca tagtcaaaca
     4321 ttttctttgc tccctgaata tctcccagca tacccagagc tgttcccttc gccacacata
     4381 tttcatgatt tccgggttca atgctttcga ggtttttcag gatcctgaga gcttcaacgg
     4441 cacgtcccct gtcgaggaga acccttgctt tcctaagctg tattgatact gaattgggat
     4501 gctgctccgc tgccagtatg gccgcttcat acgctttcag gggattgttg ctttcgatat
     4561 aataatcaat tattgattcg aattcaataa catcaaaaaa acaactttga ttattgctcc
     4621 gcatccgttc aaatttctga actgcgctcc ttatctcttc ctcaaaggaa aatccggatc
     4681 tgtcctcttc cattcccctg gcaatataat gtgcaaattt aaaaacattt caaataatcc
     4741 acttttggca gatgtgaaat tattaacaat atagtttgtt ttttcacctg aattacagcc
     4801 taatataaaa atttttaagt tttttttcac atttacgcaa ccaaatgcat gattttatca
     4861 tctatgctga tagaatatag ccaataattt ccctatatat aaaattttac cctagaacta
     4921 acctaaattc taccttaaag aaaccgggac cacccggttt ctttcttttt tctgcaacct
     4981 gattattggt taatacaccc gtgaaacagc cgattgcaca acatggcccc ggcaaacata
     5041 agctgttgga atcatcccgg gcaatcagcg gactcgtctc attgcggcaa gcagggtatt
     5101 cttcagaaga gcgaccctgg tcatgggccc gactccgccc gggactgggc taatatagga
     5161 gcactttggt gcaacgttgt cgaaatccac atcacctgca agtctccatc ctttttttga
     5221 taatggcgat tcaaccctgg ttgtccccac atctatcacc acagcgccgt cacttatcat
     5281 atcggcttta acgaagccgg gtgaaccaag agctgcaatt acaatgtctg ccttcctgac
     5341 cagctcggga atattcctgg tcctgctgtg aacaagagtt actgtggcat ccctgccctt
     5401 ttgtgaaaga agtatgctca gcggtcttcc gacaatattg ctccttccca ggattacaca
     5461 attcctgccc gaaacattta tcccatatct gtccagaagt tcaatcaccc cgaaaggtgt
     5521 agccggcaaa tagccgggca gccctgccac catcctgccc aggttaacag gatgaaatcc
     5581 gtcagcatct tttgacgggt caatcgcctc tgtcacactt gattcagaaa tatgtccagg
     5641 caatggaagc tggacaataa atgcatcaac atcatcgtct tcatttaact ccctgatttt
     5701 tgccagcagg tcgttttcag taatttcctc actcaacctc aggagggttg atttaaagcc
     5761 tacttcttca caatccttta ccttattgtt cacataggtt tcactagagg gatttctgcc
     5821 aaccagaatt gcagcaagat gaggtatctt aatccctgaa ctaaccattc gggcaacttc
     5881 cctggcaatt tccgacttga tctcggatgc gatcttatta ccatcaatta atgtgtacat
     5941 gatttatatt attttctcat taaaccggca gcattcctac ctttagtcat cattctcatc
     6001 attcgtctgg tttcatcaaa ttgtttgaga agcctgttga catcctgcac tgttgtaccg
     6061 cttcccgttg ctattctttt gcgcctgctt ccattcagga tcaccgggtt ccttctttct
     6121 ccgggtgtca tgctcctgat tatggcttca atactcttaa atgcattatc atcaatatca
     6181 aggtccttaa cagcttttcc gactccgggg atcatcgaca tcagatccct gacgtttccc
     6241 atcttcttta tctgctgtat ctgtgcaatg aaatcattaa agtcaaactg atccctggct
     6301 atttttttct gtagtttcct ggcctcttct aaatcatatt gttcctgtgc tttttcaaca
     6361 aatgacacaa tatcacccat tccgagaatc ctgtcggcca tcctcgacgg gtagaaaatg
     6421 tcgattgcat ctattttctc cccggagctt acgaatttga tcggcttatt tacgaccgac
     6481 ctgatcgaaa gggcagcacc gccgcgggta tccccatcta gtttggtaag gacaaccccg
     6541 ttgaaatcga gcctttcatt gaattccctt gcagtattga cggcatcctg accggtcatt
     6601 gaatcgacaa cgaagagaat ctcatgtggt gaaacggtat tctttatcga ggcaatttca
     6661 ttcatcatct cctcgtcaac tgcaaggcgg ccggcagtat caatgataac gacatcgtga
     6721 ttcttcatct tagcctccct cacggagttc ttcacaatct ggaccgggtt catgttgcca
     6781 tcttcagtgt ataccggaac ctccgcctgg gcggcaatga tcttcagctg ttcaattgcc
     6841 gcaggacgat atacgtcacc ggctacaaga atgggattct tccctctttt ggtcttcacc
     6901 catttcgcaa gcttgccgct gaaagtggtc tttcctgaac cctgaaggcc cgcaatgaga
     6961 atgacagccg gatttatctt tatattgata tctgatttg
//
LOCUS       DAOOYG010000141         6959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_12352_length_6959_cov_6.123117, whole genome shotgun sequence.
ACCESSION   DAOOYG010000141 DAOOYG010000000
VERSION     DAOOYG010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12352_length_6959_cov_6.123117"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(500..1318)
                     /locus_tag="PK288_10110"
     CDS             complement(500..1318)
                     /locus_tag="PK288_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPM19321.1"
                     /translation="MIFPKKRNVITVLTIVLFTASCVPNRRITMLQHTAGKGEELKAN
                     ELKARKYETGIIEYLLNPNDLLDIKISTMTPSAFNPFNDADRTLVPGMVYGQSGTLVQ
                     SQGYFIDPDGNLELPILGKLPVAGLTISQAQDSIASVVKKYLERPVVRLKLLNFRFSV
                     IGEVKNESTQLSGDNNLSLLQALGMAGGASEFGDLSKIKIIRRAGETTYVFYVNLLTE
                     DYLSSPFYYVQPNDVIVVPPLKQRPYLKYVSPNLSIFATAVSLLVAVFTLFRIK"
     gene            complement(1324..1875)
                     /locus_tag="PK288_10115"
     CDS             complement(1324..1875)
                     /locus_tag="PK288_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19322.1"
                     /translation="MKSLISLIVLAALTAGLSGQGAKRHFPSSSDLNPLRGYVNITEL
                     GGGPGLAVTSVPYSKYYFGLTTVNGYQINQYLIAGAGLGFHLYNEGWLLPLYASGRFT
                     YPIHGSKISPYANGDAGILISFGNSGDDNRLFVNPVLGARYTISPVMAADLGIGIFTQ
                     KLPGAKRDSFLNFRAGIVFIPKR"
     gene            complement(2866..3144)
                     /locus_tag="PK288_10120"
     CDS             complement(2866..3144)
                     /locus_tag="PK288_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPM19323.1"
                     /translation="MDNTGKEEILNIKHAVGLVKRNWRLFLALPVFFLALAFLVNQFS
                     RPEYRITSSILINLDNYRLQRQEINNYLNSSLLLTDPNFQNEMRILKS"
     gene            complement(3246..3635)
                     /locus_tag="PK288_10125"
     CDS             complement(3246..3635)
                     /locus_tag="PK288_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19324.1"
                     /translation="MNIKKRLAAMAVWLVARNTATCLLVLLFITSCVTQRNLGYMRES
                     SGAIRTFKANEPSDYKVKPNDELYIQINSQDEDAANIFTFSRQQSFYPGTQGPYGASL
                     LAYPVDSEGYHLLPLVGKIPGNEAFPV"
     gene            complement(4405..5514)
                     /locus_tag="PK288_10130"
     CDS             complement(4405..5514)
                     /locus_tag="PK288_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19325.1"
                     /translation="MKRLIFLLMLALASVPMLPLQSGAQILRDPESLETVKQCVDNIY
                     NFRFGEARELLQKIKRSWPGHPVGYILSGMITYWENFPLLSSTPAGDSFRDDLNACIR
                     ICGDVRNPPDYAEYLLANLGARGLLLMYYADNDLSSEVFPLARSTWRYLRESFNYTSS
                     YDDFYFFTGLYNYYREAYPDKHPVYKVIAILFPKGDREKGLTELRRAAGNSIMLKAES
                     AFFLTHIFINFENNYERGEAPGRFLHETYRGNEQYLAIYVRNLLLLRKYDEAGDLIRI
                     RNGSLANPFVRAEMTIFSGIIQEKKYGNLAEAQRLYNKGIADINPYIVYGDEIASYAY
                     FGLSRIAGMNNDRQGKKLYRKRALELTDYKSVTFD"
     gene            5790..6833
                     /locus_tag="PK288_10135"
     CDS             5790..6833
                     /locus_tag="PK288_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19326.1"
                     /translation="MKRLTVILLCIMAVTAWQCTKDERNLSLKESIERSAARINTAAD
                     AISVSKGFSILTVTDYGAKSDDNYGFRDSIDLEMIAGIYDFKPGEAKHHHHFFPLRLF
                     EKTGESDKMIINMPEKLAFHPMHLHFCDRADSVLKNNFTIRAAGYHYYFTGLNNSDYR
                     LEADFSLNSEDIGKMSMYSAWKQGFTGEYSKKFTFPEGYSVISSGSTGDTVKLIFALA
                     EGGDILLKEALILTGKDFRHHERQYILSVGNVDMKKTSGIDSIQVFLNGVLQKKAAAK
                     ITDAEADDNPSICRRRDILLTFDDGTTAKLSDLLSPARKTLKLLHRSIGEMYFSKYIV
                     DYIALSIYYNNHQ"
BASE COUNT         1780 a         1517 c         1779 g         1883 t
ORIGIN      
        1 ttttcctggg aaactggcac tggtttcagc atcagggcaa gcagaaacca gtgccagttg
       61 cccaggaaaa gatagaaata tttcataagg tcgatatcgt cctggggcgg gcgggggcgg
      121 ggcggcgcgg gttggtgtgg tgtggcggtc atggctgtgt gtttgctatg tttcgggttg
      181 cgggtttcgg gttgcgggtt tcgggtttcg ggtttcgagt ttcgggtttc gggtttcgag
      241 tttcgggttt cgggtttcga gtttcgggtg tttcgggttt cgggttttga gtttcgagtt
      301 tcgggtttcg ggtttcgggt ttcgggtttc gggtttcgag tttcgggttg cgggtttcgg
      361 gtttcgggtt tcgagttgcg ggttttgagt gtttcgggtt tcgggtttcg ggtttattgt
      421 ttaagtcttt aagtcttttg gtttttaaat ctttaagtcg catggggcat gaagcatgga
      481 gcatggggcg cggggcttat tatttgatcc tgaaaagggt gaacactgcc acaaggagtg
      541 agacggcggt ggcgaagatc gagaggttcg ggctcacata cttgaggtag ggtctctgct
      601 tcagcggagg taccacgata acgtcatttg gctgaacgta gtagaacggg ctcgaaaggt
      661 agtcttctgt taaaagatta acataaaaga cataggtggt ttcgcccgcg cggcggatta
      721 tcttgatctt cgaaaggtca ccgaactctg aggcgccccc cgccatgccc agtgcctgga
      781 ggagggacag gttgttgtcg ccggaaagct gggtcgactc atttttcacc tcaccgatga
      841 cagaaaagcg gaaattcagg agcttcagac gaacaaccgg acgctcgaga tactttttca
      901 caactgaggc gatcgagtcc tgcgcctggc tgatggtgag ccccgccacc ggaagttttc
      961 ccagtatggg aagctcaagg tttccgtcag gatcaatgaa atagccctgt gactggacca
     1021 gggtacccga ctgcccgtaa accatgcccg gcacgagggt gcggtcggca tcgttgaagg
     1081 ggttgaatgc agagggtgtc atggtggaga tctttatgtc gaggagatcg ttcgggttca
     1141 gcaggtactc aatgataccc gtttcgtact tccgggcttt cagctcattg gctttgagct
     1201 cctcaccttt cccggcagtg tgctggagca tggttatcct cctgttcgga acgcatgagg
     1261 cagtgaacag gacgatggtc agaacggtta tgacattacg ctttttcggg aagatcatga
     1321 acattatctt ttaggaataa atacgatgcc cgccctgaag ttcaggaagg agtccctttt
     1381 tgctcccggg agcttttgtg taaaaatgcc gatacccagg tcggccgcca tgaccgggct
     1441 gatggtatac cgtgcgccga ggaccgggtt aacgaataac cggttatcat cccccgagtt
     1501 cccgaaactg atcagtatcc ccgcatcacc gttggcatag ggtgatattt tcgagccgtg
     1561 aatggggtaa gtgaacctgc cgctggcata aagcggcagg agccagcctt cgttgtacag
     1621 gtgaaatccc agcccagccc ctgcaataag gtactggttt atctggtatc cgtttacggt
     1681 ggtgagtccg aaatagtact ttgaataggg tacgctggtt acagcaagac cagggccacc
     1741 gccgagttca gttatgttga cgtaacccct gagcggattg agatccgagg aggacggaaa
     1801 gtgtctcttt gcaccctgcc cgctgaggcc ggcggtaagg gcagccagaa caattaatga
     1861 gattaagctt ttcatagagg ctgattgttt aatagtggat attctggaat gtttttaagg
     1921 aaaacctctg atcgttccct gcatcctgct cccagcccca tgccctctgc cccctgctcc
     1981 aaggcctctg cccgcatcca ggcatgcttc cgccacgtca gtcacagggc cgggtgaata
     2041 caacaacctg tttaagttgc ggttatatta ttttcaacat tctgttgttt caaaaaagcc
     2101 gggtcgtgtc ctgccaacgg ctgtgcagca aatgaggttg caaacaaatg tttaatactg
     2161 ctgcgacagt cagaccggaa ctgatcatgt gttccccgaa attctttttc tttttcatcc
     2221 tgtgcccgga agtctggctg ccggctgcag aggcattcgt cagaagccat gcttgcatta
     2281 aatgttaaag tattgcttca tcaaccggtt taagttacag gccgaaaata ataaaatttt
     2341 attgaatatg ctgaaaaacc tgatgaagtt caaaggccgc agtatacaat ctgaccggaa
     2401 aatttccccc ggcggggtat ggccggaatg agcggcgctg aactgaaact ttttcatcag
     2461 ttcctctttc aggctgtgca tgtcgcgaaa ctcaaagcca taccacgccg gcgggtcgtc
     2521 agaatgatga acgggtccgg ctttatcgac aataaaggcc aggtcggctg tttgaataag
     2581 ctcaccgaaa ctggcttttt tctgaaaaag ccagtttttc ttttgtccag caaacttgcc
     2641 ggttttcagg ttataaagtg aaacttcttt tgattcagct cttacataga attgatctcc
     2701 gccgggaaat gagatgtaga acctaactcc cactggctga atatggttct ttacataatc
     2761 aattgtgaaa gggacgtttt tataggaatt gacataccat atcccggatc tgcggtagta
     2821 cctggtctga agatcgaggt tctcaattgt tttttcaaat accggttaag atttgaggat
     2881 cctcatttca ttctgaaaat tcggatcggt aagaagcagg ctgctgttaa ggtaattgtt
     2941 tatttcctgc ctctgcagcc ggtaattatc aaggttgatc agtatggatg aagttatccg
     3001 gtattccggt cttgagaact ggttcacaag gaaagccagt gcaaggaaga acacaggcag
     3061 ggcaaggaag aggcgccagt tccttttaac aagcccgact gcatgtttta tgtttagtat
     3121 ttcttcttta ccggtattgt ccatctgagt cgggggatca taaaattcaa ttcaaagtga
     3181 tgattgttaa ttatttcaca acattacaga tcaataatcc tgtagttatg gcagaaagaa
     3241 tcacttcaaa cgggaaatgc ctcattcccc ggaatctttc ccacaagggg cagcagatga
     3301 tacccttcgc tgtcgacagg gtaagccagc agggatgcac catatggccc ctgggtaccc
     3361 gggtaaaaag attgctgccg gctaaaggtg aaaatgttgg cggcatcctc atcctgactg
     3421 ttaatctgaa tgtaaagttc gtcattgggc ttaaccttat agtcagaagg ttcatttgcc
     3481 ttgaatgttc tgatagcccc ggaagattcg cgcatatacc cgagattccg ctgagtgacg
     3541 catgaggtga tgaaaagcag cacaagcaaa caggttgcgg tatttctggc aaccagccat
     3601 actgccatcg cagccagcct ctttttaata ttcattttat tcagcaaaaa gtttgtcaaa
     3661 actgtttgga cgaaattaat caattttgac aataatctga attaaaatga ataaatcccg
     3721 actcttgccc gcagcggaca gcttgccatt gaaatatcag ctgctgttca ttccccggat
     3781 gaattaaccg gatgatcttc ctccagggag aatggcgtgt cagaccggct tacttatatg
     3841 tatccttaaa aatttctggt ttatctttcc gtttcgctaa gtacatgcag gcccgcagat
     3901 gatttcaagc ctgtttgact gggtacatcc gggagattcc gtttgagtaa tcaatgatat
     3961 cccggcgatc attcaccgat ccaccggcat ctgaaagtac tgcagatctg attcaatcaa
     4021 cggataacat gatgtattca gcaaactgca ggttgaaatg gaccggatcc attcaggcaa
     4081 tgatggataa tttttcatcc ggttaccggt agaagctaca caaaaggaca tactcccgtc
     4141 acgtcagtca cattcttttt tgcctgaaac attgagacat gataaatcca tgttacctga
     4201 atttatcatt gtactggtga tattcagagt actaatagac caataccaac actttgacag
     4261 tgtttctcgg aacaggctac aataatagga aatattaagc aaagccttac agatgttgct
     4321 tttaattttg atatatggtt tgttttttta cattaaatat gcaatatcag tcatttgcat
     4381 ctttttccgg ggtgttttcc cgggtcagtc aaatgtgacg gatttataat cggtcagctc
     4441 aagcgcccgc ttgcggtaaa gctttttccc ctgcctgtcg ttgttcatcc ccgcaatgcg
     4501 gctcagtccg aagtatgcat aggaggcaat ctcatcacca tagacaatat agggatttat
     4561 gtcggcaata cccttgttgt aaagccgttg ggcctctgcc aggttgccgt acttcttttc
     4621 ctggattatg ccgctgaata tcgtcatttc cgcccgtaca aaaggatttg caagtgagcc
     4681 gttcctgatc cggataagat ccccggcttc atcatatttc ctgaggagca gcaggttcct
     4741 gacataaatt gccaggtatt gctcgttgcc gcggtaggtt tcatgaagga acctgccggg
     4801 tgcctcaccg cgttcataat tattttcgaa gtttataaag atgtgggtaa ggaagaaagc
     4861 cgattcggcc ttcagcatta tggaatttcc cgctgccctg cgcaattcgg tcaatccctt
     4921 ttcccggtcg cctttcggaa aaaggattgc aatcaccttg taaacaggat gcttgtcggg
     4981 gtatgcctca cggtagtagt tgtacagacc ggtgaaaaaa taaaaatcat cgtaggatga
     5041 ggtatagtta aacgattcgc gcaggtatcg ccaggtagac cttgccaggg gaaacacctc
     5101 gctgctcagg tcattgtcgg cataatacat aaggagcagg ccccttgcac ccagattggc
     5161 caggaggtat tcggcataat caggcgggtt ccggacatca ccgcatattc ttatgcaggc
     5221 attcaggtcg tcgcgaaaac tgtctccagc cggggttgag gagagcagcg ggaaattctc
     5281 ccagtaggtt atcattccgc tgagaatata tccgacagga tgcccgggcc atgatcgttt
     5341 tattttttgc aggagttccc ttgcctcgcc aaacctgaaa ttataaatgt tgtcgacgca
     5401 ttgtttcacc gtttccaatg attccggatc gcggagaatc tgggcgcctg actgcagtgg
     5461 cagcatcggt accgaagcca gggcgagcat cagaagaaag ataagtcttt tcaagcagaa
     5521 aaaatatctg caagtttaaa gaattttgtt acattttaca tcctgaaaca tgaaagttga
     5581 cgggttaagg agttgaaaag caggacagcg ggtgcggaca gcgaagatcc cctggcagga
     5641 ggggcagggg cgggggcggt aataacggca gcaggttgcc ggctgcaaaa cgcatataaa
     5701 tcaggctgct gtcaacattt tttcgcggaa ttaaaaattt taaaagttaa atcatacctt
     5761 tgggtatcat ttgataaatg tgagagaaca tgaaaaggtt aacggttatt ttactgtgca
     5821 tcatggccgt aacggcctgg caatgcacaa aagatgagag gaatctttca ttgaaggaat
     5881 caattgaaag gagcgctgca aggatcaaca ctgcagccga tgcaatatct gtttcaaaag
     5941 gcttcagtat ccttacggtg accgattacg gtgcaaaatc ggacgacaat tatggattcc
     6001 gcgacagcat cgacctggaa atgatcgccg gaatatatga tttcaagccg ggagaagcga
     6061 aacaccacca ccactttttc cctttaaggc tctttgaaaa aaccggggag agcgataaga
     6121 tgataataaa catgcctgag aaactggcat ttcatcccat gcatctccat ttttgcgaca
     6181 gggccgactc tgtgctgaaa aataatttta ccatcagggc cgccggctac cattattatt
     6241 ttaccgggtt gaacaactcc gattacaggc ttgaggcaga tttcagcctc aattctgaag
     6301 atatcggtaa gatgagcatg tattcagcct ggaagcaggg ttttaccggt gagtattcaa
     6361 aaaaattcac tttccccgaa ggttattcgg taatcagcag cggctcgaca ggtgatacgg
     6421 taaagctgat ctttgcactt gcagaaggcg gggatatcct tttaaaagaa gcactgattt
     6481 taacgggaaa ggacttcagg catcatgaaa ggcaatacat actatcggtc ggcaatgttg
     6541 acatgaagaa gacttcaggc attgattcga tccaggtgtt cctcaatggc gtcctgcaga
     6601 aaaaagcagc tgcaaagatc actgatgccg aagctgacga caatccgtcg atttgccgca
     6661 ggcgcgacat cctgctgact tttgatgacg gaacaacagc aaagctcagc gacctgcttt
     6721 ctccggcaag gaagaccctg aagctgttgc accggtcgat cggcgagatg tatttctcga
     6781 agtacatagt tgattatatt gctttgagca tctactataa taatcatcag taagaacgga
     6841 ggataagcgg tgagttcctc tttcgccacg gatgcggagg ataagatgcg ggtaatgagt
     6901 aatgaataat gcgaattaag agttaaaaat taagtaaatg aaagctgctg cgatttttc
//
LOCUS       DAOOYG010000142         6919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_12458_length_6919_cov_8.179050, whole genome shotgun sequence.
ACCESSION   DAOOYG010000142 DAOOYG010000000
VERSION     DAOOYG010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12458_length_6919_cov_8.179050"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PK288_10140"
     CDS             complement(<1..768)
                     /locus_tag="PK288_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPM19327.1"
                     /translation="MKNELSRRQFIQRTLGAGIALTALSPYLAAMDKKGPKGLQLGLV
                     TYMWGAKWDLPTLISNCEKSGVLGVELRTQHAHKVEINLTAAQRAEVKKRFADSTVTC
                     VGYGSNYEFHSPDPAKLRQNIDEAKEYVKLCKDIGATGLKVKPNTLPKEVEREKTIAQ
                     IAQSLNELGKFAKDYGQLIRVECHGSITQEIPNMKAIFEQVTEKNVVMCWNSNNVDLN
                     PPGLEANFNSVKQWIGDTTHIRGIRISDYPYDQLFRLI"
     gene            complement(956..2035)
                     /locus_tag="PK288_10145"
     CDS             complement(956..2035)
                     /locus_tag="PK288_10145"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HPM19328.1"
                     /translation="MKNLQMKETIGRSIILAFILSLSFSCGASRVSQQDNSSKILLVK
                     NEAERKVDVLVGGKLFTSYRWPENVYKPILYPVTTSAGTEITRGFPLNPKEGERNDHI
                     HQVGIWLNYGKVNGLDFWGNGHRGVKEPNGGEIKHVSVEKMTGGNGEAVLITNSDWLD
                     PAGVQLLAERTEYHFIIKGSTRIIDRLTTLKAGTSVVKFDDTKEGMFGIRVARQLELP
                     SDEMVALLKADGTLSATKVKASDGATGNYRSSEGILGDAVWGTRAKWMDLYGNIGTEK
                     ISIVVCDHPKNQSYPTYWHARGYGLFAANPLGWTDFTNGKQKLDFALQPGQSTTFRYR
                     VIISSGVHLTDAEINTLAEEFAKKY"
     gene            complement(2184..3545)
                     /locus_tag="PK288_10150"
     CDS             complement(2184..3545)
                     /locus_tag="PK288_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM19329.1"
                     /translation="MTKRREFIKKSALGTAAIAIGGVGFSSKSYNSISGANERITLAV
                     CGIRGQGGGHVNTWSRMKDTQNVRLKTICDVDEQYFEPRAKTVLTNTGETPKTEWDMR
                     KVFEDKEIDAVSFGTPNHWHALGTIWACQAGKHVYVEKPASYGVWEGRKMVEAARKYN
                     VRVQTGLQNRSIPGVMEAIKFMHDGGIGKVYLARGLCYKPRDSFGIAPDGTPPASLHY
                     DMWLGPAPWRPYNEKRVHYNWHWFWATGGGDTANQGPHQFDVARWGLNKNEHPVKIHS
                     MGGVFGIDPKECEQETPNTQASIFRYADGTILEFETRGRFTNAESSLGVQIGNMFLGT
                     DGYVEINGGQWKAFRKREKEPFAGSNIGERPTDLMRAPGGGSHYVNFLDAIRAGNNET
                     LHCDILDGYMSAALPALANISYRLGRELTFDGAKEKFVKDKEADKMLTREYRKPYVVP
                     EVV"
     gene            complement(3851..4894)
                     /locus_tag="PK288_10155"
     CDS             complement(3851..4894)
                     /locus_tag="PK288_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM19330.1"
                     /translation="MNKPLKRRDFIKTAAAGAITVGLAGKQVAGSGVLPQNSKRIGII
                     GLDTSHSTAFTKALNDPAAGPEFEGYKIVAAYPKGSNDIKSSVDRISGYTEDVRKMGV
                     EIVGSIDELLTKVDFVLLETNDGRLHLEQAIPVMKAGKRMFIDKPIAASLVDTLAIFE
                     ASKHYNVPLFSASSLRYITGAKEIAAGSAGKVLGADVFSPATLEKTHPDLFWYGVHGV
                     EALFTIMGTGCKTVSRAFTPDTDVVTGIWSDNRIGTMRGIRSGKSSYGGTVFGEKEIK
                     VLGSYAGYNPLLVEIIKFFRTGIPPVKPEETIEIFTFMAAAEESKFKKGAPVETEKVL
                     AKAKKKAAKISFS"
     gene            complement(5231..>6919)
                     /locus_tag="PK288_10160"
     CDS             complement(5231..>6919)
                     /locus_tag="PK288_10160"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPM19331.1"
                     /translation="SAVYQHVILNLVTKGFVVFAFDPVGQGERLEYLNPETGRSAVGG
                     PTKEHSYPGAQAFITGASQARYMIWDGIRAVDYLLTRKDVDPGRIGITGRSGGGTQSA
                     YVAAFDERIYAAAPENYITNYSRLLQSIGPQDAEQVMFHFLYRGLDHPDLLLVRAPRP
                     ALMITTTRDMFSIQGAMETEKEVTGIYKAYDASDNFSRVEDDDVHASTKKNREAMYAF
                     FRKHLNNPGSIADEVIQPLTKEELTVTPTGQLSTSMKGETVFSLNRKEAEIRLKELNA
                     ARKDPEKFSAGIISSAKILTGYNEPADVDKPVFTGRIRKEGYSIEKYFIKGEGDYVIP
                     FLLYRPVNWGSKAMLYLNPAGKSSEAGPGGEIEKFVLQGFAVLAPDILGCGETGPGSF
                     RGDAYFDGTSHNLWYASILAGRSIVGIQAGDVVKILHWMKSECHYAEISGFAVKDMAP
                     VLLHAALFDANLSGMILKEPYSSYSSIVMNRNYNTRFILSTVPEALTRYDLPDLAAAF
                     APRKLFISGMTDGNGTSFGLNNDTDIIRAGYLRKNAGDKLKIQTDLSGIEDWLK"
BASE COUNT         1678 a         1698 c         1453 g         2090 t
ORIGIN      
        1 gattaacctg aacaactggt cataaggata atcgcttatc ctgatccccc tgatatgagt
       61 ggtatctccg atccattgtt ttacagaatt gaaattggct tccagtcccg gaggattaag
      121 atccacatta ttgctgttcc agcacattac cacattcttt tcggtaacct gctcaaaaat
      181 agccttcata ttcggtatct cctgggtaat ggatccgtga cattcaactc ttataagctg
      241 accataatcc tttgcgaatt tacccagttc attgagagat tgtgcaatct gagctatagt
      301 cttttccctc tcaacttcct ttggaagggt gtttggcttg actttcagcc cggttgcacc
      361 aatatcctta cagagcttaa catactcttt tgcttcatca atgttctgac ggagctttgc
      421 aggatcggga ctgtggaatt cataattcga tccgtaaccc acgcaggtaa ctgtactgtc
      481 agcaaatcgc ttcttaacct cagcccgctg ggctgctgtc aggttgatct ccaccttatg
      541 ggcatgctgt gtcctgagct caacaccgag cactcctgat ttttcacagt tggaaataag
      601 agtcggaaga tcccattttg caccccacat atatgtaacc agccccagtt gaaggccttt
      661 cggccctttt ttatccatgg ccgcgagata aggactaagt gctgtcagag ctatccctgc
      721 tcccagggtc ctctgaataa actgacgtcg cgaaagttcg tttttcatag tctgtattta
      781 aatctgttaa tttttttatt cataacacat cctcatccca atattacccc taaatccctg
      841 cctgcgctgt cgcttcggca ggcaggcccc aagggggact tatcgatttt ccgaaacatt
      901 aaaagccccc cctgggggga tggggggtaa aaagttgtgt ctgtgcgccg tattattagt
      961 actttttcgc aaactcctcc gccagtgtat ttatctcagc atctgtcaga tgaacccccg
     1021 agcttattat caccctgtac ctgaatgtgg tcgattgtcc tggctgtagc gcaaaatcaa
     1081 gtttttgttt cccatttgtg aaatcggtcc agccgagggg attggctgca aacagcccat
     1141 aaccgcgtgc atgccagtaa gtggggtagc tctgattctt cgggtggtca cagactacaa
     1201 tcgaaatctt ttcggtaccg atattcccat agagatccat ccattttgct cttgttcccc
     1261 agactgcatc tcccaggatg ccttcactgc tcctgtaatt gcctgttgct ccatcgcttg
     1321 ccttgacttt tgtggccgac agggttccat cagctttcag gagtgcaacc atctcatctg
     1381 aaggtagctc aagctgacgg gcaaccctga tcccgaacat accctccttt gtatcatcaa
     1441 atttaacgac tgatgtgccg gccttaaggg ttgtaagtct gtcaattatc cgtgttgatc
     1501 cttttatgat gaaatgatat tcagttcttt cagcaagaag ttgaactcct gccggatcga
     1561 gccagtcaga atttgtaatg aggacagcct caccattccc gccggtcatt ttttcaacag
     1621 aaacatgctt tatctctccg ccattgggtt ctttgactcc gcgatgaccg ttcccccaga
     1681 aatcgaggcc attcaccttt ccgtaattca gccatatccc tacctgatgg atatgatcat
     1741 tccgttcacc ttccttcgga ttaagaggaa atccgcgggt gatctccgtt cctgcagatg
     1801 ttgtaacagg atagagaatg ggtttgtaaa cattttccgg ccagcgatat gaggtgaaga
     1861 gcttccctcc taccagaaca tccacctttc tctcagcttc attttttaca aggagaatct
     1921 ttgaactgtt atcctgctgc gaaactcttg atgcaccgca actgaaggag agactaagta
     1981 taaatgcgag aattattgat ctgccaatag tttctttcat ctgtaaattt ttcatagaga
     2041 tttaaaatct ttttaaccgc aaagatcgca aagtgattac gcaaagggcg ctaagtgaac
     2101 aatacaacta atttgccttt gcgtactttg cgaattcttt gcgtactttg cggttaaatc
     2161 ttaattctca gactgttacc gaattatact acttcgggaa caacgtatgg ttttctgtat
     2221 tcccttgtaa gcatcttgtc ggcttccttg tccttcacaa atttttcttt cgcaccgtcg
     2281 aaggtcagtt cccttccgag acgataggaa atatttgcca gtgccggaag agctgcagac
     2341 atatatccat caaggatatc gcaatgaagt gtctcattgt ttcctgcacg gatggcatcg
     2401 aggaaattta cataatgact tcctccgccc ggagctctca taagatctgt aggtctttcg
     2461 ccaatgtttg atcctgcgaa tggttccttt tcacgtttgc ggaatgcctt ccactggcct
     2521 ccgtttattt caacataccc gtcggtacca aggaacatat tacctatctg cactcccaga
     2581 cttgattcgg cattggtaaa ccggcccctg gtctcaaact caaggatggt cccgtcggca
     2641 tatctgaata ttgaagcctg ggtatttggt gtttcctgtt cacattcttt cggatcgata
     2701 ccgaatactc cgcccattga atgaatttta acaggatgtt cgtttttgtt aagtccccag
     2761 cgtgccacat cgaactggtg aggcccctgg ttggcagtat ctccaccgcc tgttgcccag
     2821 aaccagtgcc agttataatg aacgcgtttt tcattgtatg gtctccacgg tgccggaccg
     2881 agccacatat cataatgaag tgatgccggt ggggtgccgt caggtgcaat cccaaaggaa
     2941 tcgcggggtt tgtagcataa tccgcgtgca aggtatactt tacctattcc gccatcatgc
     3001 atgaacttga tggcttccat aactccgggt atcgaacggt tctgtaatcc ggtctgaacc
     3061 cgaacattgt atttcctggc agcctcaacc atcttgcgtc cttcccagac accatatgat
     3121 gcaggttttt ctacgtaaac atgttttcct gcctggcagg cccagattgt tccgagagca
     3181 tgccaatggt tgggtgttcc gaacgaaacg gcatcaatct ccttatcttc aaacacctta
     3241 cgcatatccc actcagtctt tggcgtttca ccggtgtttg tcaggacagt ttttgccctg
     3301 ggttcgaaat actgctcgtc aacatcgcag atggttttaa gcctgacatt ctgtgtgtcc
     3361 ttcatacggc tccaggtatt gacatgtccg cccccctgtc cgcgtatacc acagacagcc
     3421 agagtaatgc gttcattagc gccggaaatt gaattgtatg atttcgagct gaaaccaacg
     3481 ccgccaatag ctatggcagc agttcccagg gcgctttttt tgataaattc tcttcttttt
     3541 gtcatttcag ggttgatttg attatttaag ttaatgatct gaacattatt tatttactat
     3601 ttgttacttt gttaattatt taatcattca gcagattagt gatttaataa taatttttct
     3661 taaacacaaa gtgtaccacc ttagagaacg caggaacatc aactataata actccggccc
     3721 ttcttgccac cccttacccc caggggggcg ctcagattca aaagtccccc ttgggggatt
     3781 taggggtgag ttatatagta gcacaaagga tcttcaaaga gcacaaagga tggtaactgt
     3841 acaatatttt ctaactgaaa gaaatctttg ctgctttttt ctttgctttc gccaggacct
     3901 tttcggtttc aacaggagcg ccttttttaa atttactttc ctcggctgct gccatgaaag
     3961 taaagatctc tatcgtttct tcgggtttta ccggcggaat tccggtcctg aagaatttta
     4021 tgatttcaac aagaagcgga ttatagccgg catagcttcc gaggactttt atttcctttt
     4081 caccgaatac cgttccgcca taactggatt ttccggatct tatacctctc atggtaccga
     4141 tcctgttatc actccatata cctgtcacga cgtcggtatc gggagtaaag gctcttgaga
     4201 cggttttaca tcccgttccc ataattgtga aaagagcttc caccccatga acaccatacc
     4261 agaacaggtc ggggtgagtt ttttcgagtg tggccggact aaagacatct gctccgagga
     4321 ccttgccggc agaaccggct gcaatttctt tggctccggt gatataacgt aatgaggatg
     4381 cagaaaataa cggcacgttg taatgctttg atgcttcaaa tattgccagt gtatcaacca
     4441 gtgatgctgc gatcggtttg tcaatgaaca ttcttttgcc tgctttcatt accgggatgg
     4501 cctgttcaag atgaagccgc ccatcattgg tctcgagcag gacaaaatcg acttttgtaa
     4561 gaagttcgtc tattgatccg acaatctcca ctcccatttt tctgacatct tcggtatagc
     4621 ccgatatcct gtcgacgctc gactttatat cattgcttcc tttgggatat gctgccacta
     4681 ttttatatcc ttcaaattca ggtcctgcag caggatcatt cagagctttt gtgaacgcag
     4741 tactgtgcga ggtatccaga ccaattatcc ctatcctttt tgaattctgg ggaagaacac
     4801 ctgatccggc tacctgtttc ccggccaagc caacagtaat ggcgccggct gccgctgttt
     4861 taatgaaatc acgacgtttt agtggtttat tcattggggt tattgttagt ttttcgtttt
     4921 aagttaaatt attgtaaatt ttgtctttag ttggcagttg gcagttggca aaaaagttag
     4981 cagttggcag atggcagttg gcagatgaca gttggcagtt ggcagtcggc aagaagcggt
     5041 tggcaaatag ttcctttctg cgccctgagc cctgcgccct gagccctgag ccctgcgctc
     5101 tgagccctga gccgaaaagg cagccggtat tattgtaaag attcatctga aacttcggtc
     5161 ttctaaaatt agtatctttt aagacacctt atcccatcgt gtaaaaactt atgagttaaa
     5221 agaagttaaa tcatttcagc cagtcttcaa tacctgacag gtccgtttgt atttttaact
     5281 tgtcccctgc attctttctc aggtaaccgg ctcttataat gtcagtatcg ttgttcaggc
     5341 cgaaggatgt tccgtttccg tcggtcatgc ccgaaataaa gagtttccgg ggtgcgaaag
     5401 cagcagccag atcaggcagg tcatagcgtg taagcgcctc aggaacagtg ctgagtatga
     5461 atcttgtatt gtaattcctg ttcatcacta ttgaagaata tgaagaatat ggctctttaa
     5521 ggatcatccc cgataaatta gcatcaaata atgccgcatg aagaagtacc ggtgccatgt
     5581 cttttacagc aaaacctgat atttcagcat agtgacattc acttttcatc caatgcagaa
     5641 tcttcaccac atctcctgcc tgtatcccca caatgcttct ccctgccaga attgatgcat
     5701 accagaggtt atgggaagtt ccgtcaaaat atgcatcgcc acggaatgat cccggaccag
     5761 tttctccgca tcccagaata tcgggagcaa gaactgcaaa accctgcagc acaaattttt
     5821 ctatctctcc tcccggcccg gcttcagagg attttcctgc aggattaagg tagagcatgg
     5881 ctttgctgcc ccagtttacc ggtctgtaca acaggaatgg aataacataa tcaccctctc
     5941 ctttaatgaa atatttctca atcgaataac cctctttccg gatccgtccc gtaaaaacag
     6001 gtttatcaac atcggcaggt tcattatatc ctgtcagtat ttttgctgaa gaaataatgc
     6061 ctgcagaaaa tttctcagga tctttccgtg cagcattcag ctccttaagc cttatttcag
     6121 cttccttcct gttcaggctg aatacagttt cacctttcat ggaagttgat aactggccgg
     6181 tcggggttac tgtaagctct tcttttgtaa gaggttgaat gacttcatca gcaatgcttc
     6241 cgggattatt gagatgtttc ctgaagaaag catacattgc ttccctgttc ttttttgttg
     6301 aagcatgaac atcatcatcc tccactctgg agaaattatc agatgcatcg taagctttat
     6361 agatcccggt aacctctttt tcagtctcca tggctccctg gatgctgaac atatctcttg
     6421 ttgtggttat catcaatgcc ggcctgggtg ctctcacgag taacagatcc ggatgatcca
     6481 gtccgcgata aagaaaatgg aacattacct gttccgcatc ctggggtcct attgactgaa
     6541 ggagccgact gtagttcgtt atatagtttt ccggagctgc tgcatatatc ctttcgtcga
     6601 aagcagcaac ataagctgac tgggtaccac ctcccgagcg gcccgttatt cctatcctgc
     6661 cgggatcaac atctttgcgt gtaagaagat aatcgacagc ccttatcccg tcccagatca
     6721 tatacctggc ctgggaagct cctgtaataa atgcctgggc gccgggatat gagtgctctt
     6781 ttgtcggacc gccgacagct gaccgtcctg tttccggatt aagatattcg agcctttctc
     6841 cctgtcctac cgggtcgaaa gcaaagacaa caaaaccttt tgtgacaagg ttcaggatca
     6901 catgctgata tacggcact
//
LOCUS       DAOOYG010000143         6835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_12654_length_6835_cov_6.730088, whole genome shotgun sequence.
ACCESSION   DAOOYG010000143 DAOOYG010000000
VERSION     DAOOYG010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12654_length_6835_cov_6.730088"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK288_10165"
     CDS             complement(<1..215)
                     /locus_tag="PK288_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19332.1"
                     /translation="MGFSEFTSGLLENAVNEFASLPGIGRKTAFRMVMNLLKRDPADI
                     KRFGDTISKLHNEIRYCEALIWCPQIKA"
     gene            325..1785
                     /locus_tag="PK288_10170"
     CDS             325..1785
                     /locus_tag="PK288_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPM19333.1"
                     /translation="MTPGLILFVFVIYIIALFFVTWLTVRKADNQSFFIGNKKSPWLV
                     VAYGMIGASLSGVTFMSVPGWVQDTQFSYMVVVFGYLVGYIIIALVLLPLYYRLKLTS
                     IYTYLEQRFGFWSYKTGAGFFIISRVLGASLRTFLVINVLQIFVFDAWNIPFWLNVTV
                     FMVLVILYTMKGGIKTIVWTDTLQTTFMLLAVVLSVLYISKDLGVPLGRLFAEINQSD
                     MSRMFITDWHNERYFVKQFFSGMFITIVMTGLDQEMMQKNLSCPNIWDAQKNMYFFSV
                     IIVFVNFLFLVLGAMLLIYANAKGVAVAFTDELFPTIAFKYLGTVAGMVFLIGLISAS
                     FPSLGGALTALTTSVSIDFLRLDRRKDVSETSLTRFRYVIHISIAVIFVICILIFRAL
                     NDRAVIDKLFTIAGYTYGPLLGLYTFGLFVPRKVIDRATPVVAILCPIICYFLSMYSE
                     ELFNGYRFGFEILLVNGFLTFVGLLIFSRRIEPQMS"
     gene            complement(1833..2591)
                     /locus_tag="PK288_10175"
     CDS             complement(1833..2591)
                     /locus_tag="PK288_10175"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HPM19334.1"
                     /translation="MRRTCVFFISLLLFFHSVSARAQDTIEFPLKIRIGMDVAGPAIS
                     YYNRDRLSLEGVLSLDRNEKMAYVLEGGYLKFNYSQYNYDYRTEGVFMRAGVNFNLLK
                     PETSTGKYWAGVGLRYGLSLFSTEVPFFRKENYWGTYNSSLPGRTSAGHFIEVSPGVR
                     TELFKNLSIGWSVRLRLLISGGGGRDLRPIHFPGFGNSGKITNAGINYYIVWEIPFKT
                     KKVIRIPETEEEEEEETPVEGLPGNEVPQNAFPY"
     gene            complement(2563..3036)
                     /locus_tag="PK288_10180"
     CDS             complement(2563..3036)
                     /locus_tag="PK288_10180"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HPM19335.1"
                     /translation="MMKKSYRPILLLLFAAFEILIISCAPEACLEETESLVKASLYDN
                     TTKRLRSPDSLTLYGLNHETVKLYDRSRGVQPVLFPLDLSSDNCTFVIKIDSVIDTIT
                     FRYTSKPRLVSKECGYTFYFNLTDTPFYTRNAIDRIYTANRKITTYNEENLRIFY"
     gene            complement(3256..5901)
                     /gene="alaS"
                     /locus_tag="PK288_10185"
     CDS             complement(3256..5901)
                     /gene="alaS"
                     /locus_tag="PK288_10185"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPM19336.1"
                     /translation="MLNSLTANELRQLFFRFFQARGHVIVPSAPMVIKNDPTLMFTNA
                     GMNQFKDIFLGNQPPVSRRIANSQKCLRVTGKHNDLEEVGHDTYHHTMFEMLGNWSFG
                     DYFKKEAIEWGWEFLTRELGIPEDRLYVTVYEGDKDDGIPPDLESFEHWKNCCSDADR
                     QIIFGSKKDNFWEMGETGPCGPCSEIHADLRDDYERKGKPGHELVNKGHPLVLEIWNL
                     VFIQYNRKSDGTLEQLPEKHVDTGMGFERLCMVVQGRKSNYDTDLFQPLIQRISVITG
                     KKYGENENTDVAHRVIADHLRAVAFSIADGQLPSNVRAGYVIRRILRRAVRYGYTSLE
                     MEEPFIYKLVAVLADQLGATYPELVAGKEQITKIIHEEEAAFLKTLGKGLKIIEKVIG
                     ELRQTNQTTLPGKIAFELYDTYGFPVDLTRLILKENSMDLDSREFENEMKSQKNRSRD
                     DAAIVTGDWTIVRPIEGTLFTGYDNTEEEILVTRYRTIRVKGRERLQLIFDRTPFYAE
                     SGGQVGDTGFIESATEKIHISDTIRENNLIIHISNDRISDASARFYARVDLEKRRMTA
                     INHSATHLIHYALRSVLGKHVEQKGSLVTPDRLRFDFSHYAKPSREELTRVEMMVNRM
                     VRENVGRHVAENITMDQAKKMGAIALFGEKYGDTVRVVTFGDSVELCGGTHIDSTGRI
                     GIVKIVSEGAIAAGVRRLEAVTALKAEEYINEKLGYADEIASMLKITGNLKEGVEKLI
                     DENTTLKKNLEKAQVQLALSKAADLEKTQKTIRNIRIFSGQLESGPPEILKTIAYHIR
                     NKYHDCAVMIGSETEGKASLLIMISDSLVKSRNIDASEIIKKVADEIKGGGGGQPFLA
                     TAGGKNPSAIGAALKKAEEIIINMI"
     gene            6003..>6835
                     /locus_tag="PK288_10190"
     CDS             6003..>6835
                     /locus_tag="PK288_10190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPM19337.1"
                     /translation="MPRHKYFLDLHELQFKRIRYPWKARLSRLSLWIAASIVLSVFYN
                     TLFYSHFGSLKAKLLRQEIDDIKLNYSLLSRDIDNSDKLLDNLELSDGNCYRPVLDMD
                     PIPGSVRNMGIGGVNRYEELEGYENSRLMLTYAKRIEDLRNRSTLQYESFRAIEGRQE
                     EWKREMEYLPMISPVNVTYRLGDGVRFREVHPVLGTPQWHHGQDFSTPYGTEVFATGS
                     GRVIEAGWKGGLGNCIVIDHGYGYRSTYGHLSSMKVSAGLNVKRGDLIGLTGSTGTST
                     GP"
BASE COUNT         1686 a         1576 c         1492 g         2081 t
ORIGIN      
        1 gctttgattt ggggacacca tatcaaagct tcacaatacc ggatctcatt gtgaagcttt
       61 gatatggtgt ccccaaatcg cttaatgtca gcaggatccc tcttcagcag gttcatcacc
      121 atgcgaaatg ccgtctttct tccgattccc ggaagggagg caaattcatt tacagcattt
      181 tccagaagcc cggatgtaaa ttcggaaaaa cccataaaag cagctttgaa gcaaatttag
      241 tttaatatga tctttaaaac tatggatact taatattttt tacctgccga ctttttgtta
      301 tccttgcgga taaattttta agaaatgact cccggcctta ttctgtttgt tttcgttatt
      361 tacataatag cacttttttt cgtaacatgg ctgaccgtca gaaaagccga caatcagtca
      421 ttctttatcg ggaataaaaa atccccctgg ctggtggttg catatggtat gattggtgcc
      481 tcgctttcgg gcgtaacctt catgtcggtt ccaggctggg tacaggatac ccagttcagc
      541 tatatggtcg tggtttttgg ctatcttgtc ggctatataa tcatagccct ggtattgttg
      601 ccattatact accggctaaa actgacctcc atatatactt atcttgagca gcgtttcgga
      661 ttctggtcgt acaagacagg tgccggtttc ttcattatct ctcgtgtgct tggggcatca
      721 ttgcgtacct tcctggtaat aaacgtgctt cagatattcg tgtttgacgc ctggaacatc
      781 cctttctggc tgaatgttac tgtatttatg gttctggtca tcctttatac catgaaggga
      841 ggtatcaaga caatagtctg gaccgacaca ttgcagacca cattcatgct tctggcagtt
      901 gtactttccg tactatacat aagcaaggac cttggagttc ccctcggcag gctgtttgcg
      961 gaaatcaatc agagtgacat gtcgaggatg tttatcaccg actggcataa tgaaaggtat
     1021 tttgtgaaac agtttttcag cggaatgttt attactatag taatgaccgg acttgaccag
     1081 gaaatgatgc agaaaaacct cagctgcccc aacatatggg atgcacagaa gaatatgtat
     1141 ttcttcagcg taataattgt attcgtgaat tttctgttcc tggtgctggg agctatgctt
     1201 ctgatttacg cgaatgcaaa gggagtggca gtggcattta cggatgagct tttcccgacc
     1261 attgccttca aatacctcgg aacggttgcc ggcatggttt tcctgatagg actgatttcc
     1321 gcttccttcc cgagcctggg tggcgccctt acagctctta ccacctcggt aagtattgat
     1381 tttctcagac ttgacaggag aaaagacgtc tctgaaacat cccttacaag attcaggtat
     1441 gtcattcata tttcaatagc ggtcatcttt gtaatttgca tattgatatt cagggcattg
     1501 aatgacaggg ccgtaattga caagcttttc accatagcag gatataccta cggacccctt
     1561 ctgggattgt atactttcgg attattcgtt ccaagaaagg ttattgacag ggcaactccc
     1621 gtcgtggcga ttctttgccc aataatatgc tactttctaa gtatgtattc agaggaacta
     1681 ttcaatggct acagattcgg atttgagata ctgttggtta acggattcct gacctttgtg
     1741 ggactcctga tcttcagcag gcgcatagaa ccgcagatgt catgatgcgc ggggaggtgc
     1801 attcattgct gctttttcct tttgtacgtt tatcaatatg gaaacgcatt ctgcggaacc
     1861 tcgtttccgg gtaatccttc aacaggtgtt tcttcttctt cctcttcctc tgtttcgggt
     1921 atgcgtatga cttttttggt tttgaacggg atttcccaaa caatgtaata atttattccg
     1981 gcatttgtga ttttcccgct gttgccaaat cccggaaaat gtatcggtcg caaatccctg
     2041 cccccgccgc ctgaaatcag gagccttaat ctgactgacc accctatgct caggttctta
     2101 aaaagttctg ttctgactcc cggagatact tcaatgaaat gccccgcact agtccttccc
     2161 ggcagcgatg aattgtaagt accccagtaa ttctctttcc gaaaaaaggg aacttcggta
     2221 ctgaacaggc tcagtccgta ccttaaacca actcctgccc aatatttgcc ggtcgaggtt
     2281 tcaggtttaa gcaggttgaa attcaccccg gctctcatga aaacaccttc ggtccggtag
     2341 tcgtaattat attgggaata attgaatttc aggtaaccgc cttccagaac ataggccatt
     2401 ttttcattcc ggtcgagtga taaaactcct tccaggctga gcctgtccct gttgtaataa
     2461 ctgatggccg gtccggcaac atccattcct atccgtattt taagagggaa ttcaattgtg
     2521 tcctgggccc gggctgaaac cgaatgaaaa aagagcagga gactaataaa aaatacgcag
     2581 gttctcctca ttgtaagtgg ttatttttcg attggccgta tatatacggt caattgcatt
     2641 cctggtataa aagggcgtgt ccgtaagatt gaaatagaag gtataaccgc attcctttga
     2701 aaccagtcgc ggttttgacg tatacctgaa ggtaatagta tcaattacgc tgtcgatctt
     2761 aattacaaaa gtgcagttat cagaggaaag gtcaagcgga aacagcacag gctgcacacc
     2821 ccgcgatctg tcataaagct ttacagtctc atgattcagt ccgtaaagtg tcaggctgtc
     2881 aggtgatctc agccttttcg tggtattatc ataaagggat gcctttacca gcgattcggt
     2941 ttcttcgaga caggcttcgg gagcacagga tataatcagt atttcaaatg ctgcaaacag
     3001 cagaagcaat atgggcctgt aacttttttt catcatgcct tgtaagcatc aaatttacaa
     3061 aattagtctt ttcacctgtt cagggcctta tattgttaat catttcacag tagttttcgc
     3121 gcacaaatat ttataatgac gttacggccc gcatgggcgc tcatgtcagg aaagtaaacc
     3181 cgggtctgtc acacggtccg agttatttca cttgattatt gaggcaccaa acgcagacag
     3241 cccgtgtttc attattcaga tcatgttgat gataatttct tcagcttttt tcagtgccgc
     3301 accgatagcg gaaggatttt tcccccctgc tgtggcaagg aagggttgac cgcctcctcc
     3361 tccctttatt tcatctgcaa ctttctttat tatttcagat gcatcgatat tcctgctctt
     3421 tactagtgag tcacttatca ttatcagcag gctggctttt ccttcggttt ccgacccgat
     3481 catgacagca caatcgtggt atttattccg gatatgataa gctattgttt tcaggatttc
     3541 cggtggccct gattcaagct gaccggaaaa tatcctgatg ttccggatgg ttttctgagt
     3601 tttttcaaga tcggcggctt ttgacagggc gagctgtacc tgggcttttt ccagattctt
     3661 tttcagggtt gtattctcat caatgagctt ttccacgcct tctttcagat tccctgttat
     3721 tttaagcatg gaagcaatct cgtcagcata tccgagtttt tcatttatat actcttcagc
     3781 ttttaaggcc gtgactgctt ccagcctcct gactcccgcc gcaatcgctc cttcggagac
     3841 gatctttaca attcctatcc tgcctgtact atctatatgt gtaccgccgc agagctctac
     3901 tgaatcgcca aacgttacaa cccttacagt gtcaccgtat ttttcaccga aaagggcaat
     3961 agcccccatc ttttttgcct gatccatggt aatgttttcc gcaacatgac gaccgacatt
     4021 ctctctcacc attctgttga ccatcatttc caccctggtc agttcctccc gagatggttt
     4081 ggcataatga ctgaagtcga aacgcaggcg gtcgggggta acgagtgaac ctttctgctc
     4141 aacatgtttt ccaagcacac tccgcagtgc ataatgtatc agatgggtgg ctgaatgatt
     4201 aatagcggtc atcctccgtt tttcgaggtc gacccgcgcg taaaaccttg cggaggcgtc
     4261 tgagatcctg tcattgctga tgtgaatgat gagattgttc tccctgattg tgtccgaaat
     4321 atgtattttc tcggttgcag attcaatgaa tcccgtatcg cctacctgtc ctcctgattc
     4381 agcataaaag ggagttctgt caaaaatcaa ctgcagtctt tccctgccct tgaccctgat
     4441 tgtccggtag cgtgtcacca gtatctcttc ttccgtattg tcgtaaccgg tgaacaatgt
     4501 tccttcaatt ggccgtacaa ttgtccagtc gcctgttacg atggcagcat catcccttga
     4561 cctgtttttc tgacttttca tctcattttc aaactccctg gaatccagat ccatggagtt
     4621 ctccttcagg ataagccggg tcagatcgac ggggaaccca taggtgtcgt ataattcaaa
     4681 tgctattttc cccggaagag ttgtttggtt tgtctgccgg agttctccga ttactttttc
     4741 aattattttc aaccctttgc caagagtttt cagaaaagcc gcttcttctt catggatgat
     4801 ttttgttatt tgctcttttc cggcaaccag ttccggatag gtagctccca gttgatctgc
     4861 cagtaccgcg acaagcttat aaataaaggg ttcttccatt tcgaggcttg tgtaaccgta
     4921 tcttacggcc ctcctgagaa tcctgcgaat cacatagcca gccctgacat tggacgggag
     4981 ttgtccgtca gcaattgaaa aggccacagc cctcagatga tcggcaataa ccctgtgagc
     5041 cacatccgta ttttcattct caccatattt tttaccggtg attaccgaga tcctttgtat
     5101 caggggttga aataagtcag tgtcgtaatt ggatttcctg ccctgcacca ccatacacag
     5161 ccgctcaaag cccattccgg tatctacatg tttttcagga agctgttcaa gcgtgccgtc
     5221 cgatttcctg ttgtactgaa tgaaaaccag gttccatatc tccagtacaa ggggatgccc
     5281 cttgttgacc agttcatgac cgggctttcc cttcctctca taatcatccc tgagatcggc
     5341 atgaatctct gagcaaggcc cgcagggccc tgtttctccc atttcccaga aattatcctt
     5401 ttttgatccg aaaattattt gcctgtctgc atctgaacag cagttcttcc aatgttcgaa
     5461 agactcaaga tcgggtggaa tgccatcatc cttgtcgcct tcatatacag tgacgtaaag
     5521 acggtcttcg ggaattccca gttcgcgggt caggaattcc catccccact caattgcttc
     5581 ttttttgaaa tagtctccga acgaccagtt ccctagcatc tcaaacatag tgtggtgata
     5641 ggtgtcgtgt cccacttctt caaggtcatt atgcttccct gtaaccctca ggcatttttg
     5701 tgaatttgca attcttcttg aaacaggcgg ctgatttccg aggaatatgt ccttgaactg
     5761 attcatgcct gcattggtga acataagggt gggatcattt tttatcacca tgggagctga
     5821 cggtacaata acatgccccc tggcttgaaa aaatctgaaa aaaagttgcc ttaattcgtt
     5881 agctgttaaa gaatttaaca taatttaatg caataaaaag caagtaaatg agtttgtaaa
     5941 attagttttt tatcttagat ttggggtctg ctagtcaaaa atcaaatcaa cgaaagtcgc
     6001 gaatgcccag acataagtat tttcttgact tacacgagct gcagtttaaa aggatcaggt
     6061 atccatggaa agcgagactg agtcgtttgt ccttgtggat tgcggcctca atcgttttgt
     6121 ctgtttttta caatactcta ttttatagtc atttcggatc tctcaaagcc aaactgctcc
     6181 ggcaggaaat tgatgacata aaactaaact attctctttt aagcagggat attgacaatt
     6241 cggataagtt gcttgataat cttgagcttt cagacggaaa ttgctacagg ccggttcttg
     6301 atatggatcc catccccggc tctgtaagga atatggggat cggtggtgtt aacaggtacg
     6361 aggaactgga gggatatgag aacagccggc tgatgttgac atatgccaaa agaatagaag
     6421 atctgcgaaa ccggagcaca ttgcagtatg aatctttcag ggcaattgaa ggaaggcagg
     6481 aggagtggaa gcgggaaatg gagtatcttc ccatgatcag ccccgtaaat gtcacttaca
     6541 ggctgggtga cggggtaaga ttccgcgagg tgcatcctgt gcttggaacg ccacaatggc
     6601 atcacggaca ggatttcagc accccttatg gaaccgaggt ttttgccacc ggatcaggca
     6661 gggttatcga agcaggatgg aagggcggac tgggcaattg tattgtgatt gaccacggtt
     6721 atggataccg gtcaacatat ggacatctca gcagtatgaa agtaagtgcc gggctgaacg
     6781 ttaagcgcgg cgatctcatt ggccttactg gaagcaccgg aacatctacc gggcc
//
LOCUS       DAOOYG010000144         6684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_13068_length_6684_cov_7.193996, whole genome shotgun sequence.
ACCESSION   DAOOYG010000144 DAOOYG010000000
VERSION     DAOOYG010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13068_length_6684_cov_7.193996"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PK288_10195"
     CDS             <1..401
                     /locus_tag="PK288_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPM19338.1"
                     /translation="GVKRYMDESIRKAREKGYVTTMFGRRRYLRDIGSRNQVVRGNAE
                     RNAINAPIQGSAADIIKVAMVRIHQKMKTGNYKSKMILQVHDELIFEVVPNELDLLRE
                     MVIHEMTNAVKLDIPLRVDWGVGKNWLEAH"
     gene            518..751
                     /locus_tag="PK288_10200"
     CDS             518..751
                     /locus_tag="PK288_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HPM19339.1"
                     /translation="MEITVLFFGILADATGLNAKSYNGIDSFDDLRHRMEDDFPGIFH
                     YNFRIAVNRVITEDNPVLKDGDEVACLPPFAGG"
     gene            781..1191
                     /locus_tag="PK288_10205"
     CDS             781..1191
                     /locus_tag="PK288_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPM19340.1"
                     /translation="MRLIEGPLMPGLIAEVIAGSGGSHDTGAHALFLGQVRRDRIDGR
                     FVKAIEYSAYEEMVIMEANKISKTILSEFEDVRAFEILHSRGMVKAGETALLVIVSAG
                     HRQQALAACGKAVDLINERLPVWKKELFEDEPDL"
     gene            complement(1218..2951)
                     /locus_tag="PK288_10210"
     CDS             complement(1218..2951)
                     /locus_tag="PK288_10210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPM19341.1"
                     /translation="MKKKFSARLTIVAVLFMAAAISVGFLASQETRDFRIAKNLDIFL
                     SLFRELNTFYVDEINPDKFIKAGIDNMLKTLDPYTVFYPESEGDEVAFMTTGKYGGIG
                     SLIRSTGDYVVISQVYKGFPADVAGIKAGDLLKKADGVSLKGFPTEKVSEKLKGNPGS
                     TVILTIERNGEEMEIPVKREKIMVPPVPYFGMLDDRTGYIRFTNFTENCSEAVKNALV
                     ALKDKKAEQIILDLRSNPGGLLNEAVEIVNLFIGPGREVVSTKGKVKQFDEVFKTTRS
                     AVDEKIPLAVLINRSSASASEIVAGAIQDLDRGIIVGQRSYGKGLVQITRPLSYNSQL
                     KLTTAKYYIPSGRCIQALDFSHPNEDGSVGTIPDSLVSEFKTSNGRIVKDGGGITPDV
                     EVVPAPLSQISAELYLRNLIFDYATRYYWTHPEPETIEQFTFSDADYSDFTGFLKKLN
                     FSYQTATEESLNDLLINARKEKYFDLHKDLFAQLQKELGHTLEHDLTIFRKELTSLLE
                     NEIISRYFYEEGSIAWSIRTDEQVLRAVEILNQGEKYNSILQGRSGSILITRSDEKPA
                     ARQAAYSGNDH"
     gene            complement(3052..3441)
                     /gene="rnpA"
                     /locus_tag="PK288_10215"
     CDS             complement(3052..3441)
                     /gene="rnpA"
                     /locus_tag="PK288_10215"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPM19342.1"
                     /translation="MTGERQTFRKDERLCRIRLISEIFENGNVFYTSLFKVIWLRCHG
                     EFKPPAQIAVSVPKKNIRLAVSRNLIKRRIRESYRKNKSNLYSVLNRKNIQIAFMVIF
                     RGKNIPDYDSVSKSVDEMIDFLCRNAT"
     gene            complement(3522..4274)
                     /locus_tag="PK288_10220"
     CDS             complement(3522..4274)
                     /locus_tag="PK288_10220"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPM19343.1"
                     /translation="MKIKKILVSQPEPNNPKSPYFDLAKKYNLKIDFKPFITIEGVSA
                     KDFRQQKINILDFNAVIFTSKTAIDHFFRICNELRIVVPDTMKYFCITENVAFYLQKY
                     IVYRKRKIFHGKAKFQDLIDVIIKHRDSNFLVPLSEPHNAEIPGLLDSNDINYTIGTM
                     YRTISSNFSNINDFDYDILVFYSPSGIKSLKENFPDFVQGDIKIAAFGPTTACAVEQE
                     GLRLDIHAPNPKAPSMTMALDDFLKDHNRNCR"
     gene            complement(4322..5350)
                     /locus_tag="PK288_10225"
     CDS             complement(4322..5350)
                     /locus_tag="PK288_10225"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HPM19344.1"
                     /translation="MNNGLSIQDTTGTRLFIRIDAGKLLPDSVFTRMDEVPAHLAAGA
                     PSGTEKRPKAPEKVIIKDTISICRRNSIADITFYDRDVFLKNLNYIPPGWIRYKDAGN
                     RGLRMDKAEFKTAAYLREGESRSSYNAHNDLILIIILFAAIMFLQVHYMMRNFADDVR
                     RFFLFRGINEPSSREVSSLFTWQSTLLNFISFLVIALFGFTSLVFYGLNPAGVHSSLL
                     IPILLVIVIFGITMRHLICTAAGNLSGNYDVFNEYLVNVYHSYRFSAMILFALVLIMT
                     FTSLLSKELCIITGIASLGLIYFFRITRLSLIFIRRNISILYLILYLCALEILPVLIF
                     LKYFEGLV"
     gene            complement(5424..6296)
                     /gene="deoC"
                     /locus_tag="PK288_10230"
     CDS             complement(5424..6296)
                     /gene="deoC"
                     /locus_tag="PK288_10230"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPM19345.1"
                     /translation="MTKLYQKLIKLCPSENEIRKGLRRVSDLTSEARDKHLLMNILNV
                     IDLTSLNTSDNRSRIIHLTGKVNSFSGRFSNIPNVAAICVYPNFVPVVKEKLTVKNVK
                     IATVAAGFPASQTFRSVKLAECKMAVEAGADEIDVVMPVGSFLGNDFARVADEIREIK
                     EAVGDRILKVIVESGLLADYEQIFKASMIAMDAGADFIKTSTGKTSISATPEVALVMC
                     RAILDFYSETGIKVGFKAAGGITTTDDALTYYYIVSTCLGKEWLSNKLFRIGASRLAN
                     NVLADISGTRNDYF"
BASE COUNT         1861 a         1434 c         1494 g         1895 t
ORIGIN      
        1 ccggggtgaa aaggtatatg gacgaaagca taaggaaggc ccgtgaaaag ggctatgtaa
       61 caaccatgtt cggaagaagg cgctatctcc gggatatcgg gtcgcgaaac caggtggtgc
      121 gcggcaatgc ggaaaggaat gcgattaatg cacccattca gggcagtgca gctgatataa
      181 ttaaagttgc aatggtaagg atccatcaga agatgaaaac agggaattat aagtcaaaaa
      241 tgatcctcca ggtacacgac gaacttatat tcgaggtggt acccaatgaa cttgacctgc
      301 tcagggagat ggtgatccat gaaatgacaa atgccgttaa gctcgacatt ccactcaggg
      361 ttgactgggg tgtcgggaaa aactggctcg aggcgcattg ataataaatg cacctgttcc
      421 gggagaaatg aacagctgat tggttaagca tttatcaaac ctcccgcgga acaaccgaca
      481 tttaatatcc acgggtaatc gatagcagga taaaattatg gagatcacag tattgttttt
      541 tggcattctt gcggatgcaa caggcctgaa cgcaaaaagc tacaatggca ttgattcctt
      601 tgatgacctg agacacagga tggaggatga ttttccggga atatttcatt ataatttccg
      661 gatagccgtc aacagggtga ttactgaaga taatccggtc ctgaaagatg gtgatgaggt
      721 tgcatgtctt ccaccttttg cgggagggta ataatatggc aaccattaaa ttttattgat
      781 atgcgattga tagaaggtcc gcttatgccc ggtctgattg ccgaagtgat cgccggaagc
      841 gggggaagtc acgacacagg cgctcatgcc ctgttcctgg gacaggtccg cagggaccgg
      901 attgacgggc gttttgtaaa agccattgaa tactctgcct atgaggagat ggtaattatg
      961 gaagccaaca agatcagtaa aaccattctt tcggagtttg aagatgtcag ggcctttgaa
     1021 atacttcatt ccagggggat ggtaaaggcg ggagaaacag cacttctggt tattgtttct
     1081 gcggggcacc ggcaacaggc ccttgctgcc tgcgggaagg cagtggatct gataaatgaa
     1141 agactgcctg tttggaaaaa ggagctgttt gaagatgaac cggatctgtg aaagtacaaa
     1201 agcggggagc gggaacctta atgatcattc ccggaatatg ctgcctgtct tgctgctggt
     1261 ttttcatcgc ttctggtgat gagtattgaa cctgacctgc cctggagtat tgaattgtat
     1321 ttttcaccct ggttgagtat ttcaacggcc cggagtacct gttcatcagt ccttatgctc
     1381 caggcaattg aaccttcctc atagaaatac ctgctgatta tttcgttttc aagaaggctc
     1441 gtaagctctt tcctgaaaat cgtgagatca tgttcaaggg tatgtcccag ttctttctgg
     1501 agttgtgcaa aaagatcctt gtgcaggtcg aaatactttt ctttcctggc atttatcagg
     1561 aggtcgttaa gggattcctc cgttgcggtc tgataactga aattcagttt tttaagaaaa
     1621 ccggtgaaat ccgaataatc tgcgtcactg aaagtgaact gttcaatggt ttctggttcg
     1681 ggatgcgtcc agtaatagcg tgtggcatag tcaaaaataa ggtttctcag atacagttcg
     1741 gcagatatct ggctcagagg tgccggaaca acctccacat cgggtgtaat ccctccgccg
     1801 tcctttacga ttctgccgtt tgatgtttta aattctgaga ccagagagtc gggtattgtt
     1861 cctacgctcc cgtcctcatt gggatgtgaa aaatccaggg cctggatgca tctgcccgaa
     1921 gggatgtaat acttggcggt ggtcagcttc agctgtgaat tgtaactcaa cggacgagtt
     1981 atctgaacaa gccctttccc atatgatctc tgcccaacga ttattcccct gtccagatcc
     2041 tgtattgctc ctgcaacaat ttcagaagca gaggcggacg accggttgat aagcacggca
     2101 aggggaattt tttcatcgac agcagatctc gtggttttga atacctcatc aaactgtttt
     2161 acctttccct ttgttgaaac gacttccctt ccgggaccaa tgaacagatt gacaatttcg
     2221 acggcttcat tcagcaggcc cccggggttg ctgcgcaggt cgaggattat ctgttcggct
     2281 tttttgtctt tcagggcgac aagggcgttt ttgacggcct cagaacaatt ttcggtgaag
     2341 tttgtaaacc ggatataacc tgttctgtca tcaagcatgc cgaaataggg gacaggaggg
     2401 accataattt tctccctctt caccgggatt tccatttcct ccccgttcct ttcaattgta
     2461 agaataaccg ttgagccggg attccccttc agcttctcgc tgactttttc cgtaggaaag
     2521 cctttaagtg agacaccatc ggcttttttc agcagatcac cagcttttat gccggcaaca
     2581 tcagccggaa atcccttgta aacctgtgaa atgacaacat aatcaccggt acttcttatc
     2641 agcgatccga taccgccgta tttaccggtt gtcataaacg cgacctcatc accttctgat
     2701 tcgggataaa ataccgtgta aggatcaagg gttttcagca tattgtcaat gcctgcctta
     2761 ataaatttat ccggatttat ctcgtcaacg taaaacgtat taagttccct gaagagggaa
     2821 agaaaaatgt caaggttttt tgctatcctg aaatcccttg tttcctgtga tgcaagaaat
     2881 cccacactta tagcggctgc cataaagaga acggccacta ttgtcaacct ggctgaaaac
     2941 ttctttttca ttcaattgaa atcttaaaac cggaaagcga aattaatatt taattcaagt
     3001 aactggtctg ctgatgaaca aattataaca acattaacat ttgtcattca attatgttgc
     3061 attgcggcac agaaaatcaa tcatttcatc aactgacttg ctgaccgaat cataatccgg
     3121 aatatttttt cccctgaata taaccataaa tgcaatctgg atatttttcc tgttcagaac
     3181 gctgtaaagg ttacttttat tctttctgta tgattccctt attctgcgtt taataagatt
     3241 tctggaaaca gccagccgga tatttttttt cggaacgctt acggcaatct gggcaggagg
     3301 tttgaattca ccgtgacacc tgagccagat taccttaaag agggatgtat agaaaacatt
     3361 tccgttttca aaaatctcag aaatcagcct gatcctgcaa agccgttcat ccttgcggaa
     3421 tgtttgcctc tctccagtca tatcggcatt atctgacagg gaaagtgatt acctgtcatt
     3481 tggttgaaat ctccggatca gtaataatca taaagatcct gctaccggca gttccggttg
     3541 tgatccttca ggaaatcatc aagggccatg gtcatggaag gagctttcgg attcggggca
     3601 tgaatgtcca gcctcagtcc ttcctgctca acggcacatg ctgtagtagg gccgaaagca
     3661 gcaattttga tgtcgccctg gacaaaatcg gggaagttct ctttcaaaga tttgataccc
     3721 gaagggctgt aaaataccag gatatcgtaa tcaaaatcgt tgatatttga gaaattgctg
     3781 cttatggttc tgtacattgt gccgatagtg taatttatgt cgttactgtc aagaagaccg
     3841 ggaatttcgg cattgtgagg ttcggagaga ggaacaagga aattggagtc cctgtgcttg
     3901 attattacgt caatcaggtc ctgaaacttt gcctttccgt gaaagatctt ccgtttccgg
     3961 tatacaatgt acttctgaag gtaaaaagcc acattctcgg taatgcaaaa gtatttcatg
     4021 gtatccggaa ccactatcct caactcgttg catatacgga agaaatgatc aatggcggtc
     4081 ttgcttgtaa aaataactgc attgaaatcc agaatattta ttttttgctg acgaaaatcc
     4141 tttgctgata caccttcgat ggtaatgaaa ggcttaaagt caatcttgag gttgtatttt
     4201 ttagccaggt cgaaatatgg agatttcgga ttgttcggtt ctggctgtga aactaagatc
     4261 tttttaattt tcaatttctt acagatttag ttttacataa aaagcaacac ttccttcacc
     4321 gttaaacgag accttcaaaa tattttaaaa agatcaaaac cggcaaaatt tccagcgcac
     4381 aaaggtacaa aatcaaatat aatattgaaa tattccgcct tataaaaatt aatgacagcc
     4441 gggtaatgcg aaaaaagtat atcagcccca gtgaggcaat acccgttata atgcacagtt
     4501 cctttgaaag caatgaagta aaggtcatga taagaaccag ggcaaacaat atcatggcac
     4561 tgaaacggta cgaatgataa acgtttacaa ggtattcatt gaacacgtcg taattcccgc
     4621 tgaggtttcc ggctgccgta caaatgagat ggcgcattgt gatgccaaaa atgacaatga
     4681 ccagtaatat cgggataagc agtgaagagt gtacccctgc ggggttcaga ccgtaaaaaa
     4741 cgagcgacgt aaacccgaac agtgcaatta ccagaaatga aatgaaatta agaagggttg
     4801 attgccatgt gaaaagggaa cttacttccc gtgatgaggg ttcgtttatg cccctgaaaa
     4861 ggaaaaacct gcggacatca tcggcaaaat tcctcatcat gtaatgcacc tgaaggaaca
     4921 taatggctgc aaacaatatg ataataagga tcaggtcgtt gtgggcgtta tatgaagacc
     4981 ggctttcacc ttccctcaga tatgcggcag tcttaaattc tgccttatcc attctcaaac
     5041 cccggttacc ggcatccttg tacctgatcc atccgggcgg gatataattc agatttttga
     5101 gaaaaacatc ccggtcgtaa aaggttatgt ctgcaatact gttcctgcgg caaatggaga
     5161 ttgtatcctt aattatgacc ttttcaggtg cctttggtct tttttccgtc cctgacggag
     5221 cgcctgcagc cagatgagcc ggaacctcat ccattcttgt gaatactgaa tcaggaagaa
     5281 gctttccggc atctattcgg ataaacaatc ttgtccctgt ggtatcctgt atgctaaggc
     5341 cgttgttcat tcaatttttg cggagcaatc cacaaaaata gtgataatta ccttaaaaga
     5401 atcctggtcc ttttcagggg taatcagaaa taatcattcc gtgttccgct gatatcggcc
     5461 aggacgttgt ttgcaagcct gctcgcaccg atcctgaaaa gcttgtttga cagccattcc
     5521 tttcctaagc aggtactgac aatatagtaa taggttaagg catcatctgt ggttgttatt
     5581 cctcctgcag ctttaaatcc gaccttgatt cccgtttcag aataaaagtc aaggattgcc
     5641 ctgcacatta ccagggctac ctccggggtt gcagatatgc tcgttttgcc tgttgaggtt
     5701 ttaatgaaat cagcaccggc atccattgca atcatggaag ccttgaaaat ctgttcataa
     5761 tcagccagca gacccgattc aacaattacc ttcaggatcc tgtccccgac agcctccttt
     5821 atttccctga tctcatcagc tacccgggca aaatcatttc ccaggaatga tcctaccggc
     5881 attacgacat caatctcatc cgctcccgct tcaacagcca ttttgcactc tgccagcttg
     5941 acgctccgga aggtctgtga agcaggaaaa cctgctgcaa cagtcgctat tttaacgttt
     6001 ttgactgtaa gtttctcctt cactaccggc acaaaattcg gataaacaca tattgcagcc
     6061 acatttggta tgtttgagaa tcttcctgaa aaactgttta ccttaccggt gagatggatt
     6121 atcctgctcc tgttatcaga ggtgtttaga cttgtcagat caataacgtt caggatattc
     6181 ataaggagat gtttatccct ggcttccgag gtaaggtcac tcactctcct gagtcctttc
     6241 ctgatctcat tttccgaggg acataacttg ataagctttt gatataactt tgtcatacgg
     6301 taaacctgga ttaaatcttt ggtactaggt gtaaaaagaa cgggttcaat ttaatgtttt
     6361 ttgttttcaa aattaactgg aggggatttt ttaaatcacg aataaaaatg cggaacggag
     6421 aaggctttta ggatcagatt actgtgaaat atttaacagt agccggaaga ccagttcctg
     6481 acaggaaaat tcccgttgtc cctgcaagac accggagtaa gccgtgtcag tcctgcagct
     6541 ttgatgaaat accgttattc ctcgcaaggg aagcgaagat ccccagtacc gacaataccg
     6601 aaaaaatgat gaagcctgtt ctcattcctg ccaggagcct tgagtaattt gcaggattga
     6661 tctgttcttt tccgaggtaa acgg
//
LOCUS       DAOOYG010000145         6509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_13577_length_6509_cov_6.706694, whole genome shotgun sequence.
ACCESSION   DAOOYG010000145 DAOOYG010000000
VERSION     DAOOYG010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13577_length_6509_cov_6.706694"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PK288_10235"
     CDS             <1..466
                     /locus_tag="PK288_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM19346.1"
                     /translation="CETPDSLQTLYEFDGFNVLWDHAIGIDDGAYGRNHGLGFVGENG
                     TLVIDRGGWEVIPETVNGKTRMEAVPLKKGAGKGLANHVVNHLDCIARRDPNTNASIE
                     IGAHIAKFSALGNIAYRTGKKLVWDGTRFTNDTGANSYLVPEYRTPWSLPRI"
     gene            628..2466
                     /locus_tag="PK288_10240"
     CDS             628..2466
                     /locus_tag="PK288_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HPM19347.1"
                     /translation="MSFMFNPVDFDDQGAINRISLDEETAASIVSGTGESAVYISELL
                     SDLTSEKERPVIAVFDGYIGALWEQSLKLVTENLESVSVKVIPEDFRKVFKSSDLLDE
                     LFKGHLPSDREKDPVLLFGKLFKGTYDDILDDQKTDELIARLRVHRNSGGAGKVILVY
                     GCGSAIKRVRQLADYVFYLDVAPKNVILRARKGLFANLGDTAPRGIDEFIRRCYYVDY
                     EIASRHRGELLTGSHIDFYIDGNDLSDLKMMPRRAFDSIMSALVRYPVRCKPVYLEGV
                     WGGQFIRKFRNLPRSVRNVAWAYDLIPLEVSIVVAYGKTGIEFPFFTFVQKEGLNLMG
                     KECVEKFKGYFPVRFNYDDTWHSSGNMSIQVHSGHEYNVENFDELGRQDEGYYVVATG
                     HGARTFAGFSEDADVDEFICEVKKSETEHTVVDYEKYVNYVRSYPGMQVLLPAGTIHA
                     SGRNQVVLETGSLTVGSYTYKLYDYLRPDLNGRPRPIHTWHGERVLMKDRKTAWVREN
                     LVRPPVIVRKGEGWAEYIVGEHDLLYFSLRRFEFEKKIEDDTRGRFHVLTLVDGEKVI
                     IRSAENPELFYEQYFLDVVIVPANMGRYIVKNEGNQPVCIHKAMLK"
     gene            2844..5483
                     /locus_tag="PK288_10245"
     CDS             2844..5483
                     /locus_tag="PK288_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPM19348.1"
                     /translation="MKNRVPFLVILFSIAFLTAVTAQEFPFRNTALTVEERVNDLVSR
                     LTLQEKADQLLYTAPAIPRLGIPSYNWWNEALHGVARAGFATVFPQSITIANSWDEGL
                     MYNVASAISDEARAKYHEFQRRGKTGIYHGLTFWSPNINIFRDPRWGRGHETYGEDPF
                     LTGRMGLQFVRGMQGDDPVYLKTVATAKHYAVHSGPEPLRHSFNARVSEVDLRETYLP
                     AFRTLVVDAGVYSVMGAYNMFRDHPCCASPELFGILRNEWGFNGYFVSDCWAISDFYM
                     FTRSAKDPAEAAAMAVKAGTDLECGVDYKHLTEAFDRGLLTENDIDRAVKRVFTARFK
                     LGMFDPDEKVSYAQIPFFANCSDYNKSLSRLAARKSIVLLKNSDNILPLPREIRTLAV
                     IGPNADNFESLIGNYNGIAKDPVTVLRGVCNKVTPDTKVLYAEGSDLAEGIHNLEVIP
                     SRYLQTPEGTQGVTGFYYDNREMKGEPVFTRTDDRIDFYWEHFSPSPALPDDDFGVRW
                     ITFLIPPETGRYSLGSWGSSAYEIFLDGEKIISEKSEHHAFHKEYMTYLEAGRKYRIE
                     VHYRNYEGDADIKLLWARPRPNIIEEAVRAAKEADAVILVLGLSQRLEGEEMGIKIDG
                     FLGGDRTNLNLPDTQEKLFDAVTAAGKPVIVVLMNGGALAVNNVQEKAAAVILAGYPG
                     QEGGNAVADVLFGDYNPAGRLPVTYYKSIDQIPAFEDYNMNGRTYRFFTSEPLYPFGY
                     GLSYTSFAYSGLSIPQKANVGEKVKVSVKVTNTGPRDGEEVVQLYLADVKATTPRPVR
                     QLEGFERIFLKKGESRIVEFILEPRQFSIINNKDKRVIEPGYFTISVGGKQPGFSGYL
                     DPQCTGVLTGRIRLAGKEEKFDN"
     gene            5551..>6509
                     /locus_tag="PK288_10250"
     CDS             5551..>6509
                     /locus_tag="PK288_10250"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPM19349.1"
                     /translation="MQHENHLLKKRVTLFDGISIVAGAMIGSGIFIVSADIARTVGSP
                     GYLLVVWIITGLLTVIAAASYGELASMMPDVGGQYVYLREAYHPLVGFLFGWTTFLVI
                     QCGSIAAVAVAFAKFSGVLFPWISDRNVLTIAGPVKLTSTMVVAIAMIVFLTWLNTLG
                     IVTGKTVQNFFTSTKVIALFGFILIGFLATRGLNSYEINKEVFWQAGQPGPGNQVVPL
                     AGFALIAAIATALVGSLFSSDAWYDVTFISGEVINPKKNVPLSLVLGTALVSVIYLLT
                     NLVYIRILPLSGSPDGTDILTRGIQYATDDRVATSAMSVVFGD"
BASE COUNT         1738 a         1404 c         1676 g         1691 t
ORIGIN      
        1 ctgtgaaacg cccgacagct tgcagacact gtatgaattt gacggattca atgtcctttg
       61 ggatcatgcc atcggcattg atgacggagc ttacggacgg aatcatggcc tgggatttgt
      121 cggtgaaaac ggcaccctgg tgatcgatcg cggcggctgg gaagtcatcc ctgaaactgt
      181 gaacggaaaa acacggatgg aagccgtacc tctgaaaaaa ggggctggaa aggggctggc
      241 aaaccatgtt gtaaatcatc ttgattgcat tgccaggcgg gatcccaata ccaatgcaag
      301 tatagagatc ggggcccata tcgccaaatt ctcggcactc ggtaacatag cttaccgtac
      361 cggaaagaag cttgtatggg atggtaccag gttcaccaac gatactgggg ccaacagtta
      421 tcttgtgcct gaataccgga ccccgtggag tttgccgcga atctgaccgc tacaggcaat
      481 tattgcttta actgttattt cagggaactg ttcaaataat ccggatacct tacgaaagct
      541 ccggacaata agaacggacg ggatttcctg aaggtgcttt taccagcatt gaaaaaagat
      601 attcattgat aaatcaagtg gaagattatg agcttcatgt tcaatcctgt tgactttgat
      661 gatcagggag caatcaacag gatatctctg gatgaggaaa cagcagcttc gattgtttcg
      721 ggaaccggag aatctgcagt ttacatctcg gaattactct cagatctgac tagtgaaaag
      781 gaacgtccgg tcatagcggt tttcgacggt tacatcggag ccttgtggga acagagcctg
      841 aaactggtaa ctgaaaacct cgaatcagta tctgttaagg tcatccctga ggacttcagg
      901 aaggttttta aatcatccga tcttctcgac gaattgttta aagggcacct gccttcagac
      961 agggaaaagg atcctgtcct tcttttcgga aagctgttta agggaactta tgatgatatc
     1021 ctggatgatc agaaaacgga tgagttgatt gcaaggctcc gggttcacag gaattccggc
     1081 ggagcaggaa aagtgatcct tgtatatgga tgcgggagtg ccataaagcg ggtcaggcaa
     1141 ttggcggatt atgtttttta ccttgatgtt gcacccaaaa acgtaatact cagggcaagg
     1201 aaaggactgt tcgccaatct tggggacact gcccccaggg gaatcgatga gtttataaga
     1261 cgctgctatt atgtcgatta tgagattgca tcgagacacc ggggtgaact tctgacaggc
     1321 agtcatatcg atttttatat tgacggcaat gatctgtcgg acctgaagat gatgccccgc
     1381 cgggcttttg actcaataat gtcggcactg gttagatatc cggtgagatg caagcccgta
     1441 taccttgaag gtgtatgggg cggccagttt atcaggaaat tccggaacct gccccggtcg
     1501 gtcaggaacg tagcctgggc ctacgatctg atcccgctgg aagttagcat cgtggttgct
     1561 tatggaaaga ccggtattga gttccctttc ttcacatttg ttcagaaaga aggtttaaac
     1621 ctgatgggga aggaatgtgt tgaaaagttc aaaggatatt tcccggtgcg gtttaattat
     1681 gatgatacat ggcacagcag cgggaacatg tcgatccagg tccattcagg gcacgaatac
     1741 aatgttgaga acttcgatga gctcgggaga caggatgagg gttattacgt ggttgccacc
     1801 gggcatggag caaggacatt tgcaggtttc agcgaagatg ccgacgtgga tgaattcatc
     1861 tgcgaggtaa aaaagtcgga aacggagcat acggttgtcg attatgaaaa atatgtaaat
     1921 tatgtcaggt cttatcccgg gatgcaggta cttttaccgg caggaacgat acatgcatca
     1981 ggccggaacc aggtggtact ggaaacagga agccttaccg tcggatccta tacatataaa
     2041 ttgtatgatt atctgcgacc cgatctcaac ggcaggccca gacccataca tacctggcac
     2101 ggggaacgcg tcttgatgaa agacaggaaa acggcctggg taagggaaaa ccttgtcaga
     2161 cctcccgtga tcgtcagaaa aggagaagga tgggctgaat atattgtcgg ggaacatgac
     2221 ctcctttatt tcagcctccg aagatttgaa ttcgaaaaga aaattgaaga tgacacccgg
     2281 ggcaggttcc atgttctcac ccttgtcgat ggcgaaaagg tgattatccg gtctgcagaa
     2341 aatcctgaat tattttatga gcaatatttc cttgacgttg tcatcgtacc tgcaaatatg
     2401 ggcaggtata ttgttaagaa tgaaggcaat cagcctgtct gcatccataa agcaatgttg
     2461 aaataagtta acttaaagag gccggtgaaa agcattattt cactgatgat catatttaaa
     2521 ctgcagggaa aaattattcc ggataatttg tactggttcc gtgaaagcat tcaatcgcag
     2581 ggtagcagtg aaattgatga aaaaacctgt catgtgaact ggtatgtgcg gcctgcccat
     2641 gaatgattct gtacaatatt taaaattagc tggcggatcg atattaattc ggctcttttt
     2701 catacatttc accgcttttt tggtctgatt tcagaaaaca gccggtaaac aaaacctcca
     2761 tagcatgcaa caagttcgat ccctgatact ttcatttgta caaacgacag aagactgacg
     2821 ctgaaaactt aaaaatcatt gaaatgaaaa acagagttcc ctttcttgtt attttatttt
     2881 caattgcctt tttaaccgcc gttacagccc aggagttccc attccgtaat actgcactca
     2941 cggttgaaga gagggttaac gacctggttt cgagactgac tcttcaggaa aaggctgacc
     3001 agttattgta cactgctccg gcaattcccc ggttggggat cccttcctat aactggtgga
     3061 acgaggctct tcacggagtt gccagggcgg gctttgccac cgtttttcca caatcgatca
     3121 ccatagccaa ttcatgggac gaagggctga tgtacaatgt tgcaagtgcc atatcggatg
     3181 aggccagggc aaagtaccat gagtttcagc ggaggggaaa gacaggtatt taccatggtc
     3241 taacattctg gtcaccgaat atcaacattt tccgtgaccc gagatgggga aggggacatg
     3301 aaacttatgg tgaggatcct ttcctgaccg gacgcatggg actgcagttt gtcaggggaa
     3361 tgcagggtga tgaccccgtg tacctcaaaa cagtggccac cgcgaaacac tatgcggtgc
     3421 acagcggccc tgaaccactc aggcattcat tcaatgccag ggtgagtgaa gttgacctaa
     3481 gggaaactta tcttcctgct ttcaggaccc tggtggttga tgccggggta tattcggtaa
     3541 tgggggcgta taatatgttc agggatcatc catgctgtgc aagccctgaa ttgttcggga
     3601 tactgcgtaa tgaatgggga tttaacggct attttgtatc cgactgctgg gctatttccg
     3661 atttctatat gttcaccagg tctgcaaaag atcctgcaga agccgctgct atggctgtca
     3721 aggccggtac cgacctggaa tgcggggtcg attacaagca tcttactgaa gcctttgacc
     3781 ggggattact gactgaaaac gatattgacc gagccgttaa gagggtgttt accgccaggt
     3841 ttaaactggg tatgtttgat cccgacgaaa aagtatctta tgcccagatc ccgttttttg
     3901 ccaattgctc ggattataac aaatcacttt caaggcttgc agcccggaaa tccattgttc
     3961 ttctgaaaaa ctcggacaat atcctgccgc ttccacgaga aatccgtacc cttgccgtta
     4021 tcggaccaaa tgccgataat ttcgaatctc tcattgggaa ttacaatggc attgccaaag
     4081 atcccgttac ggttctcagg ggtgtctgca ataaagtcac acctgataca aaagtacttt
     4141 atgctgaggg aagcgatctg gcggagggaa tacataatct tgaagtcatt ccttcacgtt
     4201 accttcagac acctgaaggt acccaggggg taaccggttt ttattatgat aacagggaaa
     4261 tgaagggaga acctgttttt acccgtacag atgacagaat agatttttac tgggagcatt
     4321 tttctccttc ccctgctctt cctgatgatg attttggcgt cagatggata acatttctca
     4381 tccctcctga gacgggacga tacagcctgg gaagctgggg atcgtcagcc tatgagatct
     4441 tccttgacgg agaaaagatt atttcggaga aaagtgaaca tcatgcattt cacaaggaat
     4501 acatgactta cctggaggca ggcaggaagt accggataga ggttcattac aggaactacg
     4561 agggagatgc ggatataaaa ctgttgtggg cccgcccgcg tccgaatatt attgaggagg
     4621 ctgtcagggc agcaaaggaa gctgatgctg tgatcctggt cctgggcttg tcgcagcggc
     4681 tggaaggtga ggagatgggc attaagatag acggattcct tggaggtgac cgtacgaatc
     4741 tcaatctgcc ggatacccag gaaaaactgt tcgatgcagt aacggctgca ggaaaaccgg
     4801 tcattgtcgt tctcatgaac ggcggggccc ttgctgttaa caacgtgcag gaaaaagcag
     4861 ctgccgtgat ccttgccggc taccctggcc aggaaggagg gaatgccgtt gctgatgtcc
     4921 tgttcggtga ttacaatccg gccggccgcc tgccggtgac atattataaa tcaattgatc
     4981 agataccggc ttttgaggat tacaacatga atggcagaac atacagattt tttaccagcg
     5041 aaccattata tccgttcgga tatgggctga gctacaccag ctttgcctat tccggcctgt
     5101 caatcccgca aaaagccaat gtgggggaaa aagttaaggt gagtgtgaag gttacaaata
     5161 caggtccaag ggatggcgag gaagtcgtac agttgtatct tgctgatgtt aaggcaacca
     5221 cacctcgccc tgtacggcag ctcgaaggct ttgaaaggat attcctgaaa aagggcgaaa
     5281 gccggatagt ggaattcata ttggaacccc gtcagttttc aattattaat aacaaagaca
     5341 aacgtgtaat tgaaccgggt tattttacta tatccgtcgg aggcaaacag ccgggattca
     5401 gcggatacct cgatccccaa tgcacgggcg tgctgactgg tcggataaga ctggccggaa
     5461 aggaagaaaa atttgacaat taagatcatg ctggtgctga ttgcgctaac ctctgctgtt
     5521 ctacaccgga tatctgaaaa gctttaattt atgcagcacg agaatcatct cctgaaaaaa
     5581 agggttactc tctttgacgg tatatcaata gtggccggag cgatgatcgg ttccggcatt
     5641 ttcattgtta gtgccgacat tgcaagaact gtcggttcac ccggttatct gctggtggtt
     5701 tggatcataa ccggactgct gacggtaatt gcagcagcca gttatggaga gcttgcatcg
     5761 atgatgcccg atgtgggagg tcagtatgta tatctcaggg aagcttatca cccgcttgtt
     5821 ggtttcctgt tcggatggac aactttcctg gttatccagt gcggttccat tgctgcagtg
     5881 gctgtagcct ttgcaaaatt cagcggagtc ctgttcccat ggatttccga tcgcaatgtt
     5941 ctgacaatag caggtccggt gaaactgaca agcacaatgg tcgtggcaat agccatgatc
     6001 gttttcctga cgtggctgaa tacccttggt attgtgacag gaaaaacagt tcagaacttt
     6061 tttacttcaa cgaaagtcat tgcacttttc ggttttatcc ttataggctt tttagcaacc
     6121 cggggattaa attcctatga aataaacaag gaagtattct ggcaggccgg tcagccgggt
     6181 cccggtaacc aggttgtgcc actggcaggt ttcgcactca ttgcggccat tgcaacagcc
     6241 cttgtaggat ccctgttctc ctctgatgcg tggtatgacg tcacctttat ttcgggtgag
     6301 gttataaacc ctaaaaaaaa cgtacccctg agccttgtac tgggtacagc acttgtgtct
     6361 gtcatctatc tgctgactaa cctggtttat atcaggatcc tgcctctctc aggatctccg
     6421 gatggaacgg acatacttac ccggggcatt cagtatgcta ccgacgaccg tgtggctacc
     6481 tcagccatga gcgtcgtttt tggcgacta
//
LOCUS       DAOOYG010000146         6487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_13653_length_6487_cov_6.592040, whole genome shotgun sequence.
ACCESSION   DAOOYG010000146 DAOOYG010000000
VERSION     DAOOYG010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13653_length_6487_cov_6.592040"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PK288_10255"
     CDS             complement(<1..193)
                     /locus_tag="PK288_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19350.1"
                     /translation="MPLILDRNHVLDVYAEAAGRKWVIPAFNAENLTTTEAILSAAME
                     YGAGTGQPDIPVTIGITNLY"
     gene            complement(290..1672)
                     /locus_tag="PK288_10260"
     CDS             complement(290..1672)
                     /locus_tag="PK288_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19351.1"
                     /translation="MIKTKAGFIVYGVHKDGLKDPMGQPFIDEKIVAGAKKALQNAGI
                     ELVEHDLVIASKKEARECFDRFKKMNDVDALILFSGTWVWAAHMIAAIRDYSTTGKGI
                     VIWTNPGSQGWRPVGGLVLQGALKETGIRHRFVYGSFEDQAEIEKITSYCRASAMRNR
                     LNMSTIGTFGGRGMGQTCGAADPSQWMRMFGIDIDSRDTSELLKTAREIPAEEVKKER
                     TRIQKLFSEPIPADDQADRSIRLYLAIKKLVKAYEWDFYTIQSFPGLGSDYSATCFAQ
                     SLMLEDRVGTSTLGDFNTALTVKCLTDLSDEPVYYGDLQHVDKSNNEIKIIGDGACPP
                     SLAGKLGPAGFAGHGIPTEGEAGGLSVKLVCKVGEGVLARLGRNNGRFEMVIARCTIF
                     EPPADQIEKRKNECGIPFWPHGFVRAHCDIEQLLQSWNNEYACLAYGSHLYEELRAFC
                     ELTGINAIAL"
     gene            complement(1686..2765)
                     /locus_tag="PK288_10265"
     CDS             complement(1686..2765)
                     /locus_tag="PK288_10265"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM19352.1"
                     /translation="MTKNIYLVTRRTNIDKASFPVIDAHNHLWGNWQVEKVVETMDRA
                     GVVSYCDLTGNVRIEFKEGGYRISPGDISDFFDNCAEKYPGRFYCFTMANLAQPADKP
                     LFTDHRRFVEEFIEMLNMHVNKGAKGLKVLKELGLRYRDAGGSLIPCDDQRLFPLWEE
                     AGRLGIPVLIHQADPAGFFEPVTPENEHYDNLKKYHSWSFADPSFPRKTELLKQRDNL
                     VKQHPGTVFILPHFANYAENIQYVSGLLDENPNVYIDFSARIDELGRQPYSSREFFIR
                     YQDRIIFGTDMPANIDDSLEMYRTYFRFLETFDESFYSPDYDGTFGYARWPVCGIGLP
                     REVLKKIYSENILRIIPSLKSEITI"
     gene            complement(2861..5983)
                     /locus_tag="PK288_10270"
     CDS             complement(2861..5983)
                     /locus_tag="PK288_10270"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HPM19353.1"
                     /translation="MLTKPMQMMICTLLLLCSSVIILDGQKATIREYKKVFTTYPYSD
                     PDPVPLATSLYPYFRYEGFTSKPVQQAWKVVELENDYIKVIILPEIGGKVWTAIEKST
                     GKPFIYNNQVIKFRDIAMRGPYTSGGLELNYGIIGHTPNCATPVDYLVVENSDGSVSC
                     ILGVLDLLTRSNWRLEVRLPADKAFFMTKSFWYNSTPINQPYYHWLNGGYKASGNLEF
                     IFPGTCHIGHDGEYAAWPVDETNGRTISFYESNNFGGYKSYHVFGKYSDFFGAYWHDD
                     GFGIARYGTHDDKAGKKIWIWGLSGQGMIWENLLTDSDGQYVEMQSGRLFNQNSQGST
                     FTPFKHLGFAPYGTDTWTEYFYPVLKTRGFVAANDFGALNIKAENGFLKLYLSPVQSV
                     DEILEVKEGGNTVYRKKVKLNPLKTFSDSVRFNGDDRNLAVSLGTNLLNYSTDPGYNV
                     LSRPVEAPRDFDWNSSYGLYIQGKEAMDQKMYPLAETKLEASLEKDPYFLPSLVKLAE
                     LNYRNMMYPRALDLITKALSIDTHDGAANYYYGLINLANGRITDAKDGFDIASISAEY
                     RSAAYTGLSRIYFSEGNYDRAFAYAIKATDFNRFNIDALQLQAAISRCRGNHKKAREI
                     LDSILSFDPLNHFARFEKYLLDDTGDAKKNFLELIRNELPHETLMELAVWYYNSGCRD
                     EAGKMISLLPAIPEKEYWRAFLTGTKADLDGINPDYSFPFRSETGIVIGQLLKNQDDW
                     LLKYHLALIYRDRNRRDESVKLLMSCGDTPGYAPFYAARAAVMRGTDSHQCEADLKKA
                     LTLDNQWRYHVLLAGYYIDRQQYDKALAVTGPYYRLHPDNFITGSLHARTLLLNRKYR
                     DADAVLRKLVILPHEGATSGREMYREAKLMQALDAMEKKKTGEAMRFISEAGLWPENL
                     GVGKPYDQDIDTRLEDWLAYLCLQRQKKTDEAGKMLRKIKDFKPSVENGVRNFIPVNT
                     LISAWALERLGSKTDARNFIGEQKKKFPGYSAIDWSMAVFEQDRSFSMPENSRDANVR
                     IIERLVDSGL"
     gene            complement(6091..>6487)
                     /locus_tag="PK288_10275"
     CDS             complement(6091..>6487)
                     /locus_tag="PK288_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19354.1"
                     /translation="IRRIEMGYGAIALKAIAKTLSMTLKIAQAAYERKVPCFCADLTV
                     NPILVEWNKNVAARLDSFPGLGMLGLVESNGHQNYRNWQAMMDYHPAKNAEWVKINRG
                     VYELNDAFYNTGGGIFEPSAHYEEMFSGV"
BASE COUNT         1567 a         1598 c         1438 g         1884 t
ORIGIN      
        1 tgtacagatt ggttattccg attgtgaccg gaatatcggg ctgtcctgtt ccggcaccat
       61 attccatggc agcagagaga atggcttcag tggttgtaag gttttctgca ttgaacgccg
      121 ggatcaccca ttttctcccg gctgcttcag catatacatc gagtacatgg tttctgtcaa
      181 gaatcagtgg catgtataat agaattacta tgattaagga ttacggattc cggatcacat
      241 tacggcttac tgcagctgat ggtgtttcgg aatgcaacgg ggataatcat tacaatgcta
      301 ttgcatttat acccgtaagt tcacagaatg ccctgagctc ttcatagaga tggcttccat
      361 aggccaggca ggcatattca ttgttccatg attgcagaag ttgttcgata tcgcagtgtg
      421 ccctgacaaa tccgtgtggc cagaatggta ttccgcattc gtttttacgt ttttctatct
      481 ggtcggccgg tggttcaaaa atggtgcagc gtgcaatgac catttcaaac cggccgttat
      541 tccttccaag ccgagccagc actccttctc ccactttgca aaccagttta acggaaagtc
      601 ctccggcttc accttcagtc ggaatgccgt gcccggcaaa tccggcagga ccgagcttgc
      661 ctgccaatga gggagggcac gcgccgtcgc caataatctt gatctcattg tttgatttgt
      721 caacatgctg cagatcgccg taatataccg gctcatcgct caggtctgtt aaacatttaa
      781 cagtaagggc tgtattaaaa tctccgaggg ttgaagtgcc caccctgtct tcaagcataa
      841 ggctctgtgc aaaacaggtt gcactgtaat cacttcctaa gcccgggaaa gattgaattg
      901 tgtagaaatc ccattcataa gctttcacca gttttttaat ggcaagatag agccttattg
      961 accggtcagc ctgatcatca gcaggaattg gctccgaaaa aagtttctga attcttgttc
     1021 tttccttctt tacttcctca gcagggatct ccctggctgt tttaagcaat tcgctggtgt
     1081 cgcgggaatc aatgtcaatt ccgaacattc tcatccactg tgatgggtca gcagccccgc
     1141 atgtctggcc cattcccctg cctccgaaag ttcctattgt actcatattc aatctgtttc
     1201 tcatcgcgct tgcacggcag taagaggtaa tcttttctat ttccgcctga tcttcaaagc
     1261 tcccatacac aaaccggtgc cgtatacctg tttccttaag cgctccctgc agtaccagac
     1321 ccccgacagg cctccatccc tggctgcccg gatttgtcca tatcacaata ccttttcctg
     1381 tggttgaata atcacggatt gcggctatca tatgtgcagc ccatacccac gttccggaaa
     1441 aaagaatcag ggcatcaaca tcattcatct tcttgaacct gtcgaagcac tccctggcct
     1501 ctttcttgga ggcaatcacc aggtcatgtt caacaagttc aattcctgca ttctgcagcg
     1561 ccttttttgc accggcaacg atcttttcat caataaatgg ctgtcccatc ggatctttca
     1621 atccgtcctt atggacacca tacactataa aaccagcttt tgttttaatc atggtcaact
     1681 tattattaaa tggttatttc agatttaagc gaaggaataa ttctcagtat gttctcagaa
     1741 tatatttttt taaggacctc ccggggcagt ccgatcccgc aaaccggcca gcgggcatat
     1801 ccgaaggttc catcgtaatc gggagaatag aatgattcat cgaaagtttc caggaacctg
     1861 aaatatgtcc tgtacatttc aagggaatcg tcgatatttg cgggcatgtc ggtcccaaaa
     1921 atgatcctgt cctggtatct gataaaaaat tcccttgacg aatagggctg ccgtcctaat
     1981 tcgtcaatcc ttgccgagaa atcaatataa acattcgggt tttcgtccag cagccctgac
     2041 acatactgaa tattttcagc gtagttggcg aaatggggaa ggatgaatac agttccggga
     2101 tgttgtttaa ccaggttgtc cctttgtttg agcaattctg tcttccgggg gaaggatgga
     2161 tctgcgaagc tccatgaatg gtatttcttc agattatcat agtgctcatt ttccggggtt
     2221 accggttcaa aaaatcctgc gggatctgcc tggtgtataa ggacaggtat tcccagtctt
     2281 cctgcttctt cccataaagg aaacagacgc tggtcatcgc atggaataag tgatccgccg
     2341 gcgtcgcggt aacgtaatcc cagttccttc aatactttca gcccttttgc tcctttgttt
     2401 acatgcatgt taagcatttc gatgaactcc tccacgaagc gcctgtggtc agtgaataga
     2461 ggcttgtcag caggctgggc aaggttagcc atggtaaagc aataaaaccg tcccggatat
     2521 ttttccgcac agttatcaaa gaaatctgag atatcacccg ggctgatcct gtagcctcct
     2581 tctttaaact caatccttac attgcccgta aggtcgcaat aactcaccac accggctctg
     2641 tccatggttt caaccacttt ctcaacctgc cagttgcccc agagatgatt atgggcgtca
     2701 attaccggga aggatgcctt atcaatgttt gtacgcctgg ttaccagata gatattcttc
     2761 gtcatagagc tcaggttttg atcacttttt caattcattc cacaccctga tgcagcgcgt
     2821 aaagttaccg tactcctgta ttttccatgg aataattcat tcacaacccg gagtcaacga
     2881 gcctttcaat tatcctgaca tttgcatccc tgctgttttc aggcattgaa aaactcctat
     2941 cctgctcaaa cactgccatg ctccagtcaa tagcgctgta accgggaaat ttctttttct
     3001 gctccccgat aaaattccgg gcatcagtct tactgcccag tcgttcaagt gcccaggccg
     3061 atataagggt atttacgggg atgaagttcc tgacgccgtt ttcaacggaa ggtttaaaat
     3121 ccttaatttt acgaagcatt tttccggctt cgtctgtctt tttctgtctt tgcaggcaaa
     3181 gataggcaag ccagtcttcg agccgggtat caatgtcctg atcatagggt tttccaactc
     3241 ccaggttttc cggccaaagg ccggcttcgg agataaatct cattgcttcg ccggtctttt
     3301 tcttttccat ggcatccagg gcctgcatga gttttgcttc ccgatacatt tccctgccgc
     3361 ttgtagctcc ttcatgaggc agaataacca gtttcctcaa gacagcatct gcatcacgat
     3421 atttcctgtt cagcaggagt gttcttgcat ggagagaacc tgtaatgaag ttgtcggggt
     3481 gcagcctgta ataaggtccg gttactgcca gggctttatc atattgctgc cggtcaatgt
     3541 agtaaccggc cagaagaaca tgataccgcc actgattgtc aagagtcagg gctttcttaa
     3601 gatctgcctc gcactgatgg ctgtcagttc ctctcataac tgcggcacgg gccgcataaa
     3661 aaggggcata tccgggtgtg tcaccgcatg acataagcag cttcacactc tcatccctgc
     3721 ggttcctgtc cctgtatatc agtgccaggt gatatttaag cagccagtca tcctggtttt
     3781 taagcaactg cccgatcact atacctgtct ctgatctgaa tgggaaggag taatcgggat
     3841 tgatcccgtc cagatcagct tttgtacctg tcagaaatgc cctccagtat tccttctccg
     3901 gtatggcagg cagcagggaa atcatttttc cggcctcgtc cctgcagccg gagttataat
     3961 accacactgc cagttccatc agggtttcat gggggagttc atttcttata agctcaagga
     4021 aattcttttt tgcatccccg gtatcgtcaa gaagatattt ttcaaaacgg gcaaaatgat
     4081 taagcggatc aaatgaaaga attgaatcaa gaatttcccg ggccttttta tggtttccac
     4141 ggcatcttga aatggctgcc tggagttgca gggcatcaat attgaaacgg ttgaaatccg
     4201 tggctttgat ggcataggcg aaagccctgt catagttccc ttcgctgaaa tatatccggc
     4261 tcagtccggt ataggccgca ctgcgatact cagccgatat cgatgcaatg tcaaatccat
     4321 ctttggcatc cgtaattctg ccattggcaa ggtttatcag cccgtagtaa taatttgctg
     4381 ccccgtcatg ggtgtcgatg ctcaatgctt ttgttatcag gtccagggcc cggggataca
     4441 tcatgttacg gtagttcagt tcagccaatt tgaccagcga aggcaggaaa taaggatctt
     4501 tttcaagtga agcttcgagt tttgtttccg caagcggata cattttctga tccatcgctt
     4561 ccttaccctg aatatacagg ccatatgagc tgttccaatc gaaatcgcgc ggtgcttcaa
     4621 caggccttga aagcacgttg tatccggggt cggtgctgta gttcaacaaa ttcgtgccca
     4681 gcgatacggc cagattcctg tcgtcgccat tgaacctgac cgaatcactg aaagttttta
     4741 gagggttcaa tttcactttt ttcctgtaaa cggtgttccc gccttcctta acttccagga
     4801 tttcatcaac ggactgaaca gggctgagat ataacttcag aaatccgttc tcagccttta
     4861 tgttgagtgc tccaaaatcg tttgctgcga cgaaccccct tgttttaagg acaggataga
     4921 aatattcggt ccaggtatca gtgccataag gtgcaaaacc gagatgtttg aacggagtga
     4981 atgttgaacc ctggctgttc tggttaaaaa gccgtcccga ctgcatttca acatattgtc
     5041 cgtcactgtc ggtcagcagg ttttcccaaa tcattccttg tccggaaagg ccccatatcc
     5101 atatcttttt tccggctttg tcgtcatggg tgccgtaacg ggctattcca aacccgtcgt
     5161 cgtgccagta tgcaccgaaa aagtcagaat atttcccaaa gacatggtac gatttatatc
     5221 cgccgaaatt attggactca tagaaggaaa tcgtcctccc gttggtttca tccaccggcc
     5281 aggctgcata ttcgccatcg tgaccaatgt gacaggtgcc agggaaaata aactcgaggt
     5341 tgcctgaggc cttgtatccg ccgttaagcc agtgataata gggctggttg attggagttg
     5401 aattgtacca gaacgatttt gtcataaaaa aggctttgtc ggcaggtagt cttacttcca
     5461 gccgccagtt gcttctggtc aggagatcga gtacgcccag aatacagctt acactcccat
     5521 cgctgttttc caccacaagg taatcgacag gagtggcgca attgggtgta tgtccgatta
     5581 tcccgtaatt cagttcaagc cctccgctgg tataggggcc gcgcattgca atgtccctga
     5641 attttataac ctgattgttg taaatgaacg gtttgcctgt cgatttttct attgctgtcc
     5701 ataccttacc acctatctcc ggcaggatta tgacttttat gtaatcattt tccagctcca
     5761 caaccttcca tgcctgctga accggtttgc ttgtgaagcc ttcgtaccgg aaatagggat
     5821 agagtgatgt agctaacgga accggatcgg ggtctgaata cggataggtt gtgaaaacct
     5881 ttttatattc cctgatcgtt gctttctgac catcgaggat gataacggaa gaacacagga
     5941 gcagaagtgt acagatcatc atctgcattg gttttgtaag catagcagta ttttttgtaa
     6001 gtaagatgga actatttgcg ggtcggtctg ctgaaatata gcctgatacc cggcagggtc
     6061 atccaggatc agccggccgg cccatttcat tcatacgcct gaaaacattt cctcataatg
     6121 agcagaaggt tcaaatattc ctcctcctgt attatagaat gcatcattca gttcataaac
     6181 accccggttt atttttaccc attcagcgtt tttcgctgga tgataatcca tcattgcctg
     6241 ccagttcctg tagttctggt gtccgttgct ttcaacaagt ccgagcatgc ccagaccggg
     6301 gaacgaatca agcctggcag caacattttt gttccattcg accaggatgg gatttacagt
     6361 aaggtcggca cagaagcagg gcactttgcg ttcataggca gcctgggcaa tcttaagtgt
     6421 catgctgagt gtttttgcaa ttgcctttaa tgcaattgct ccgtatccca tttctattct
     6481 cctgatc
//
LOCUS       DAOOYG010000147         6299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_14240_length_6299_cov_6.062940, whole genome shotgun sequence.
ACCESSION   DAOOYG010000147 DAOOYG010000000
VERSION     DAOOYG010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6299
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14240_length_6299_cov_6.062940"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK288_10280"
     CDS             <1..220
                     /locus_tag="PK288_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPM19355.1"
                     /translation="SPDGKWIVFISFPSDIEVNSHPSYKRVMLRLMPVSGGKPRVIAY
                     LYGGQGTINVPSWSPDSRQIAFVSNSGK"
     gene            249..1016
                     /locus_tag="PK288_10285"
     CDS             249..1016
                     /locus_tag="PK288_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPM19356.1"
                     /translation="MVLNFKTVTLPDRRLAAARLNQGLYSLLTVPVFAMLYAITAAGL
                     FLVIIISLSGGRKIVRFLIGKWSELVFLLMGKKLFVSGRENIRKGEKYILLANHASLF
                     DIVAITSFYPEVSWFGHERLLRIPVFGQFLRLTGYVPFREPTVRNTRNMVRQLMERAG
                     SGSVALFPEGTRTLDGRINPFYRGFIYLFRTRDISILPVTLNGFYELKPKNRFYIDFG
                     SKLSVVIHKPLGKEELTGKSDDEIINIVKSVIESASH"
     gene            1026..1952
                     /locus_tag="PK288_10290"
     CDS             1026..1952
                     /locus_tag="PK288_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HPM19357.1"
                     /translation="MQEDKWIFIVNPTAGNGFAGTLVGEIEKMIPEHHAEGEVVLTKY
                     PGHASELAGEYHARGFSNIIGVGGDGTINEIAQTLAGVENVTLGVVPAGSGNDFIQIL
                     GFPGRFGKKEWDTLFRRNTIRMDAGRVNGKIFLNGMGLGFDAQVASENYIEPGVLKKG
                     GSRKYLWHIIKNLFFFRERQMVISNDGITSETDCFINTVSIGRRFAGGFLLTPEAIAN
                     DSLLDVCAIRRLNLFQRFRLLLKVPAGKHIFDKRVSYYRTAGLRIGFTDEVPFHIDGE
                     LFYAKTFDIDILAEAFNLIYNPEGNHFFRKAE"
     gene            2196..3911
                     /locus_tag="PK288_10295"
     CDS             2196..3911
                     /locus_tag="PK288_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5597 domain-containing protein"
                     /protein_id="HPM19358.1"
                     /translation="MTRISLLKLVLFFVLIAAGCGSNEDTGNKKDPGNPGSHIPYLEK
                     HGSAARLVVGGKPMLLISGELHNSTCGGLEYMRPVWKRMAEKNLNCVIATVSWELTEP
                     EKGKFDFTLVDSIITGAREAGLKLVLIWFGSWKNGGSIYIPSWVKKDYQKYPRVKDEN
                     GKTLEILSTFGEASCQADAEAYARLMQHIRMVDEKYGTVIMMQVENEAGILDNMGETP
                     GNARRDFSETANSAFNGPVPGELMDYLVHHRDDLFPELLNVWKRNGLKTTGTWEEVFG
                     KGVLRPLEKDWKFYSYYTEELFMAWNYARYIERIAAAGKKEYPIPVYVNAWLKQPTTR
                     WPGRYPSGGPLPQVIDVWRAGAPSVDFIAPDIYIDDFRWVCDEFTRSGNPLFIPETRG
                     GEAGAARAFFAFGEYGAGCFAPFGIDNPAYGNNDPLDGAYAVLGNLSQLILEHQGNGT
                     MRGILVDERTREQELFLGGYRIRASLANYSASGMTGGLIVNTGAGEFLAAAKNLDLFF
                     YPENGPERIGIDAVDEGTFINGRFIPSRRLNGDETHASTWSGTGTRFQGNDYTIQKIS
                     LYHYR"
     gene            complement(3959..>6299)
                     /locus_tag="PK288_10300"
     CDS             complement(3959..>6299)
                     /locus_tag="PK288_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HPM19359.1"
                     /translation="EGTGAISHMSVRNRPQIFNEPLMFAAISVKGAENGALVLEGPVP
                     EWKRFGQPNSGNGSPGTTFGLPRFENAVFTARFPYAMIDLNDPDIPVEVRITAWSPFI
                     PTDEDNSSLPVGGIEYTFRNTGKSAVEAVFSYNTVNFMSVQGGRAAIKPLKNGFILSE
                     DGSRERPETKGSMAIFTDVPETVVDHCWFRGGWWDPLMIAWNTIMQGGMRNTAPVESS
                     APGASLYVPLSLDKGESKTIRLMFAWYVPDTDLKYGQDVKSDDARLKEPDCCSDLPFY
                     KPWYTSMFNSIEEIVKYWQTNYDELNRKSGLFTDAFYSGSLPPEVIEAVAANLTILKS
                     TTVLRQHDGKLWNWEGCSDNSGCCAGSCTHVWNYAQAIPHLFPKLERTLRETEFNQSQ
                     DIDGHQTFRAALPIRPVATTFYAAADGQLGGIMKVYRDWHISGNTDWLRRIYPKVTVS
                     MNYCIETWDPEKKGTLEEPHHNTYDIEFWGPDGMCTSFYLGALRAVSEMGKSLGEDVS
                     RYEELYKKGKQFMEADLYDGEYFIQKIQWKGLHAKDPVEASIGSWSSDYSEEAKLLLE
                     KEGPKYQYGKGCLSDGVLGSWIAEVCGLKNIIDPVKVTSHLNSVFRYNFKTTLKNHSN
                     PQRPTYAFGDEGGLLLCTWPKGEMLSLPFVYCNEVWTGIEYQVASHLMMMGEVEKGLK
                     IVQTARSRYDGRFRNPFNEYECGHWYARALASYALLQGLTGVRYDAISRTLYMNSRTG
                     DFTSFISTETGFGNTGLKGGKPFLNVVYGEIPVTEFVVI"
BASE COUNT         1635 a         1498 c         1574 g         1592 t
ORIGIN      
        1 ttcacccgac gggaaatgga tcgtattcat ttcattcccc tccgatatag aggtaaacag
       61 ccatccctca tataagcgtg taatgctcag gctgatgccg gtttcgggcg ggaaacccag
      121 ggtgatagct tatctctacg gcggacaggg tacgataaat gtgccttcat ggtcccccga
      181 cagccggcag attgcttttg tgagcaattc gggaaaatag gtaaagaaca ataagttaat
      241 tcagtgaatt ggtcctgaat ttcaaaacag taactcttcc tgaccgcagg cttgctgctg
      301 cacgcctgaa ccagggacta tattccctgc ttaccgtccc ggtttttgca atgctatatg
      361 ccataacagc agcagggttg ttcctcgtga taataatatc cctgtccggg ggaaggaaaa
      421 tagtccggtt ccttatcggg aagtggtcgg aactggtctt cctgctgatg ggaaagaagc
      481 tttttgtctc cggcagggag aatataagaa aaggagaaaa gtacatcctt cttgccaatc
      541 atgccagtct tttcgatatt gttgcaataa cgtcctttta tccggaagta tcatggtttg
      601 gacatgaaag gcttttaagg atccctgtct tcggacaatt cctcaggctt accggatatg
      661 tgcccttcag ggagccaacg gtcaggaaca caaggaacat ggtcaggcag ctgatggaaa
      721 gggccggttc gggttcggtt gcgcttttcc ctgaaggcac caggactctt gatggcagga
      781 taaatccttt ctacaggggc tttatctacc tgttcaggac cagggatatt tccattcttc
      841 cggttaccct gaacggcttc tatgaactga agcccaagaa caggttctat attgattttg
      901 gttcaaaact cagtgtcgtc atacacaagc ccctgggaaa agaagaactg actggcaaaa
      961 gtgacgacga aattattaat attgtaaaat ctgtaatcga atcagcctcc cattgaatat
     1021 tgcgcatgca ggaagataaa tggattttca tagtaaaccc tacggccggg aatggcttcg
     1081 caggaaccct tgtcggggaa attgaaaaaa tgataccgga gcatcatgcc gaaggagagg
     1141 ttgtactgac caaatatccg gggcatgcat cagagctggc cggtgagtat catgccaggg
     1201 gattttcaaa catcataggt gtgggcggcg acggaacaat caatgaaatt gcacaaaccc
     1261 ttgcaggtgt tgagaacgtt acattgggcg ttgtcccggc aggatcaggt aatgatttca
     1321 ttcagatact gggatttccc ggccggttcg gcaaaaagga atgggatacg cttttccgta
     1381 ggaacacaat caggatggat gccggaaggg ttaacggcaa gatttttctc aacgggatgg
     1441 gactgggttt tgacgcccag gttgcttccg aaaactatat tgaaccgggt gtcctgaaaa
     1501 agggtggttc gcggaaatat ctgtggcaca ttattaaaaa tctttttttc ttcagggaaa
     1561 ggcaaatggt gattagtaac gatggcataa ccagcgaaac cgattgcttt atcaacaccg
     1621 tgtcaatagg ccgccggttt gccggtgggt tcctgcttac gcccgaagcc attgccaacg
     1681 attctctcct cgatgtttgt gcgatcaggc ggctgaacct gtttcagcgg ttcaggttgc
     1741 tgctgaaggt tcccgccggg aagcacatat tcgacaaaag ggtgtcttat taccgtacag
     1801 caggactgag aatcggattt accgatgagg tccccttcca tattgacggt gagttgtttt
     1861 acgcaaaaac cttcgatatt gatattctcg cggaggcctt taacctcata tataaccctg
     1921 aagggaatca ttttttcaga aaagctgaat gaccggatcg aaattcatga ttttctgata
     1981 aaggcaacta ctgtgaaaga aataacagca acccggaaca aacatggcac cagaaagcca
     2041 tcaggttaaa tactccgatg atcctgcacg catgcaaccc ggcaaatggc cggatggaac
     2101 cccggacaag aaaccgctaa ataaatatac ggaatcccga cagttataaa taatgaatcc
     2161 aggaacaaga acagtcaatt aaacccaggt aaatcatgac gagaatatca ttattaaagc
     2221 tggtattatt cttcgttctc attgcagccg gatgtggcag caatgaagat acgggcaata
     2281 aaaaagatcc cggcaatccc ggttctcata taccgtacct tgaaaaacat ggatcagctg
     2341 cccggctggt tgtgggaggg aaaccaatgc tccttatttc cggggaactt cataattcga
     2401 cctgcggcgg gctggaatat atgcggcctg tatggaaaag aatggctgaa aagaatctta
     2461 actgcgtgat tgcaactgtc tcatgggaac tgacggaacc ggaaaaggga aaattcgatt
     2521 tcacccttgt cgacagcatt ataaccggag cacgtgaagc cggcctcaag cttgttctga
     2581 tatggttcgg cagctggaaa aacggcggat cgatatatat accgtcatgg gttaaaaagg
     2641 attatcaaaa atatcccagg gtaaaggatg aaaacgggaa gacccttgag attctgtcaa
     2701 ccttcggtga agcttcatgt caggcggatg cagaagccta tgcacggctc atgcagcata
     2761 tcaggatggt tgatgaaaaa tatggcaccg tcataatgat gcaggttgaa aacgaggcag
     2821 ggatccttga caatatgggt gaaacacccg gcaacgccag gcgggatttc tcggaaactg
     2881 ccaattcagc gtttaacgga cctgttccgg gagaacttat ggattatctc gtgcatcaca
     2941 gggatgatct tttccctgag ctgctgaatg tctggaagag gaacggtctt aaaacaaccg
     3001 gcacctggga ggaagtcttt ggaaaaggag ttctcaggcc cctggaaaag gactggaagt
     3061 tctactccta ttacaccgag gagctcttta tggcatggaa ttatgcaagg tatattgaac
     3121 ggatagctgc agccggaaaa aaggaatatc ccattccggt atatgtaaat gcatggctga
     3181 aacaaccgac gacaagatgg ccggggcgat atccgtcagg cggccctcta ccccaggtaa
     3241 ttgacgtttg gagagcagga gccccgtcag tcgatttcat tgcccccgac atttacatag
     3301 acgatttcag atgggtatgt gatgaattta cccgcagcgg caatccgctc tttatccccg
     3361 agaccagggg aggagaagca ggagcagcaa gggcgttttt tgcattcggc gaatatggtg
     3421 ccggttgctt tgctcctttc ggaatagata atcccgccta cgggaataat gatccgctcg
     3481 acggagcata tgccgtactc ggtaatttat cacagcttat cctggagcat cagggtaatg
     3541 gaaccatgag gggcatactg gtggatgaga ggaccaggga gcaggaactg ttcctgggcg
     3601 gctacaggat cagggcatcc ctggcaaatt attcagcatc cggtatgacc ggaggattaa
     3661 tagtcaatac aggtgccggt gagttcctgg ctgccgctaa aaatctggat ttattctttt
     3721 accccgaaaa cggtccggag agaattggta tcgatgcggt ggatgaagga acattcatca
     3781 atggccgatt tatcccatca aggaggctta acggcgatga gacccacgca agtacatggt
     3841 cgggtaccgg gacaagattt caggggaatg actacactat tcagaaaatc tcactctatc
     3901 actacagata acaatgtttc cgttgtgaca ggtacgcgaa gagtccggat ggcattaatt
     3961 atatgaccac aaattccgta acgggaattt cgccgtaaac aacattaagg aaaggttttc
     4021 cccctttaag tcctgtattt ccgaacccag tttccgttga gataaatgaa gtaaaatctc
     4081 cggtccgcga attcatgtac aatgtccgcg atatggcgtc atacctcact cctgtcagtc
     4141 cctgcagaag ggcatagctg gccagggccc ttgcatacca gtggccgcac tcgtactcgt
     4201 tgaacggatt cctgaaccgg ccgtcatacc tgctccgtgc tgtctgaacg attttaaggc
     4261 ctttctcaac ttcccccatc atcatcaggt gagatgccac ctggtattct attcccgtcc
     4321 agacctcatt gcaatagacg aacggaagtg aaagcatttc acctttcggc catgtacaga
     4381 ggagaagccc accctcatct ccgaatgcat aggtggggcg ctgcgggttt gaatgattct
     4441 taagggttgt cttgaaatta taacggaata ccgagttgag gtggcttgtt acttttaccg
     4501 ggtcgatgat attcttcagt ccgcatacct cggcgatcca tgatccgagc accccgtcgg
     4561 agaggcagcc tttgccgtat tggtatttcg gaccctcctt ctccagcagc agctttgctt
     4621 cttccgaata atcgctgctc cacgatccga tagaagcctc gacgggatct ttggcatgca
     4681 gccctttcca ctggattttc tgaatgaagt actccccgtc atacaggtca gcttccataa
     4741 actgttttcc ctttttgtaa agttcctcat aacgggaaac atcctcacca agcgattttc
     4801 ccatctcgga tacggccctg agggctccca ggtagaagct ggtgcacatt ccgtcgggtc
     4861 cccagaattc tatgtcatat gtattatgat gcggctcctc aagcgttccc tttttctcag
     4921 gatcccatgt ttctatgcag taattcatgc ttaccgtcac cttgggataa attcttctga
     4981 gccagtcagt attgccgctg atgtgccagt cgcggtacac tttcataata cctccgagct
     5041 gtccgtcggc agcagcgtaa aatgtggttg ccaccggtcg tatcggcagg gccgccctga
     5101 atgtctgatg tccgtcaata tcctggctct ggttgaactc ggtctccctg agtgttcgtt
     5161 cgagcttagg aaaaagatga ggtatggcct gggcatagtt ccaaacgtgt gtgcatgatc
     5221 ctgcgcaaca tcccgaattg tcgctgcagc cttcccagtt ccatagcttc ccgtcatgtt
     5281 gtctgagaac cgtcgtcgat ttcaggattg tcaggtttgc agccacggct tcaataacct
     5341 ccgggggaag ggagccggaa taaaaggcat ccgtgaataa tcccgatttc ctgttcagct
     5401 catcgtaatt tgtctgccag tactttacta tctcttcaat ggaattgaac atggaagtat
     5461 accatggttt atagaagggc aggtcggaac agcaatcggg ttccttcagc ctggcgtcat
     5521 ccgacttcac gtcctgtcca tatttgagat cggtgtcagg cacgtaccat gcgaacatga
     5581 gccttatagt cttcgattcg cctttatcga gggataaagg cacatacagt gaagcaccgg
     5641 gggcagagga ttcaacaggt gcggtattcc tcatcccgcc ctgcatgata gtgttccagg
     5701 caatcatcag cggatcccac cagccgcccc tgaaccagca atgatcaacc accgtttcgg
     5761 gtacatcggt gaaaattgcc atgcttcctt ttgtttcagg cctttccctt gaaccatcct
     5821 ccgaaaggat aaacccattc tttaatggtt taatggctgc cctgcctccc tgtaccgaca
     5881 tgaagttcac cgtgttatag gaaaaaacgg cttctaccgc cgatttgccg gtattcctga
     5941 aagtatattc gatccccccg actggaaggc ttgaattatc ttcatcggta gggataaagg
     6001 ggctccaggc ggtaatgcgg acctcaaccg gtatgtcagg atcgttcaga tcaatcatgg
     6061 catagggaaa ccgggcagtg aatacagcat tttcaaatct cggaaggccg aacgttgtgc
     6121 ccggcgatcc gttgcctgaa ttcggctggc cgaatctttt ccattcgggt acgggtcctt
     6181 caagcacgag tgccccgttt tctgctccct tcaccgagat ggctgcaaac atcagcggct
     6241 cattgaagat ctggggtctg ttcctgaccg acatatgtga aatcgcaccg gtaccttca
//
LOCUS       DAOOYG010000148         6254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_14371_length_6254_cov_6.108082, whole genome shotgun sequence.
ACCESSION   DAOOYG010000148 DAOOYG010000000
VERSION     DAOOYG010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14371_length_6254_cov_6.108082"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK288_10305"
     CDS             <1..190
                     /locus_tag="PK288_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19360.1"
                     /translation="AAIFCHVMAENGRRTVHSSPLPPSLFHIEGGELLCQLKEPVTNL
                     KQFQALPLFAAAERGTRE"
     gene            244..1245
                     /locus_tag="PK288_10310"
     CDS             244..1245
                     /locus_tag="PK288_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19361.1"
                     /translation="MPDFTLDTYKKLLKAFLNADYSFRTFAQYLEEPGGRFIILRHDV
                     DAKKLNSLETALIENKLGITGTYYFRIVPDSFDGEIIRQISDLGHEIGYHYEDVSMIS
                     MRELRRFQGGGRIKNNGSGSEENRQEEKCEKSRKESVASGKNQKRDEMEKQLARLAFA
                     SFRENLRIFQRIVPVKTICMHGSPLSRRDNRLLWKYYDYRKLGIRGEPYFDLNFADVL
                     YLTDTGRRWDGESVSIRDKKPAEKGHSSPEGSGFSHKSADNTLNYKQPFNGHRIPSLH
                     SSFDIIRAAQANYLPDRIMITVHPQRWTNKPLPWLRELIWQNVKNTGKWALARVRGR"
     gene            complement(1331..1717)
                     /locus_tag="PK288_10315"
     CDS             complement(1331..1717)
                     /locus_tag="PK288_10315"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPM19362.1"
                     /translation="MEKYLRLYRYVFWTGYFAVLIATFLPISFRVEKIRLGPEAFSIR
                     TDHLLHFTVYFLICMFYLFGERKGLILFDKNPLKRFILLVMILATVTEVAQIWVPARA
                     FNIFDWVANMAGILAGWGIIRIAGKR"
     gene            1916..2914
                     /locus_tag="PK288_10320"
     CDS             1916..2914
                     /locus_tag="PK288_10320"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPM19363.1"
                     /translation="MPSPEEKLLLSICRLEFGENETGNLKLLADEIKDWMQFANLANK
                     HGIIALIAYNVRATNLANLIPDNVNRLLNDGLMQSILRNTWLTERWKEVNRILTDAGI
                     KHVLLKGMALEHTVYGSRGLRQMTDNDILISREDGVKAWELLQEHGFVTQPVKSPLHR
                     KIIADIGKHLPTLVKDGYALEIHLRLSGFGKAASDPANIMDNALPIKIDGITAFIPDD
                     ATHIKYLAEHSIYHQTISGPELKLFLDISLLKKDGYESINPRIFTDPSAYKNPGYKKK
                     IYREQVLGLSHGKRLRFLAGDIFPSPVWMRQRYKCSLAGVLIRYPARLWKLTWLLF"
     gene            3139..3768
                     /locus_tag="PK288_10325"
     CDS             3139..3768
                     /locus_tag="PK288_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19364.1"
                     /translation="MKNAIVILRNLLIITAVAGLFACEGPDGPMGPAGPKGDIGPAGP
                     AGPTGETGQAGKDGNANVTVISLLSKDITWLEGEYLRRPANFYSIEDAPVDDDIIDHG
                     VVLGYCSVYDNWQILPLTWENSDGTERQYILHSYSPNTITLYAYQTSGVMEPDPLIIT
                     EYRFMLITDKTVTGTKGVPAEKNILMKLEKAGVNINNYHEVLNYFGLRH"
     gene            4093..>6254
                     /locus_tag="PK288_10330"
     CDS             4093..>6254
                     /locus_tag="PK288_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19365.1"
                     /translation="MKNLFYKCLLFSITILMTAFYSDPVSGQVTAIKDWTSVYSGNSS
                     STQNINYPVPAGTNANRLMVVAIASEITAAVTQNVTLTYGGQPLTLAVGDFGTSSAFH
                     TGFYYLNEAGLDAAGINTTLSVMVTGGTVRLTDVWAAVFDHVNQTTPITDARNHNSRG
                     IYSTSFSFNPPLQVNSFNKALEVVGIFNSGLNQFRTVTYADQWEMIFESTRSDGEGLG
                     SYYSIKNCLAGRNTPVSNLTDAAEISFDKSARVSITGISLNYTPPPPPTIQASNVTFS
                     NITSSSFTVNWTNGNGANRLVIVREGSPVDAAPADGTTYTASNNWTGGQEISPGNYVV
                     YNGTADSVNVVNLDGGKTYHVAVFEFSGPPGMEDYLTVPGPARGNVTTLPETAVNGDY
                     RSAASGNWGSPASWQVYHDGAWTTASSAPSSSDGFITIISGHTITVAENVTADQVVIN
                     AGGTLHVNSGITLTIADNAGPGNDTIDCSVSGTLYVAGTLVPNGEFAFNRGSVYHHAT
                     DGGTIPAAEWDTESQCLVTGITNTAPAGLGQSFGHFTWNCASQSSALTVSFNNDITIK
                     GDFTITSTGTGKLALTSNPPQVSMTVAGNYTQTGGTFILNNSASSTVTDSLYVAGNFL
                     FTGGTITETSNTGRGEIIFNGSGAMQMYTSGGTFSETIDFLVEPGAYLQLGTGATPGY
                     ILSSKGKFTLSPGATLGITSPYGITKTSTTNPNGGNIRV"
BASE COUNT         1811 a         1507 c         1454 g         1482 t
ORIGIN      
        1 ggcggccatt ttctgccatg taatggcaga aaatggccgc cgcaccgtgc actcatcccc
       61 cctgccccct tctctatttc atatagaagg gggagagctt ctgtgccagc ttaaagaacc
      121 ggtaaccaac cttaaacaat tccaggccct ccccctcttt gcggcagcag agagggggac
      181 aagggaatga gtacatactg cgaagcaaac atttcgtaaa tttgcattgc attaaaccgg
      241 ttcatgccag atttcaccct tgatacatat aaaaagcttc tgaaagcttt cctcaacgcc
      301 gattactcct tcaggacatt cgcccaatac cttgaagaac ccggcggacg cttcattata
      361 ctgagacatg atgtcgatgc taaaaagctt aattcactgg aaacagcact tatcgaaaac
      421 aagctgggaa taaccggaac atactatttc aggatcgtcc ctgatagctt tgacggggag
      481 attatccgtc agatatctga cctgggtcac gagatcgggt atcattatga ggatgtgagc
      541 atgatatcga tgcgggaatt acggcggttc cagggcggcg gaagaattaa aaacaatgga
      601 tcaggatcag aagaaaacag gcaggaggaa aaatgtgaaa aatcacggaa ggaatccgta
      661 gccagcggga aaaaccagaa aagggatgag atggaaaagc agcttgcccg gctggctttc
      721 gcaagtttcc gggaaaacct ccggatattt caacgaattg ttccggtaaa aacaatatgc
      781 atgcacggaa gccccctgag caggcgggat aatcgcctgc tctggaaata ttatgattac
      841 aggaagctcg gaataagggg agaaccttat ttcgacctca acttcgctga tgtcctctat
      901 cttacggaca caggacgtcg ctgggacggt gaatctgttt ccataaggga taaaaaacct
      961 gctgaaaaag gtcattcttc tccggaaggt tccggctttt cacataaatc tgcagataat
     1021 accttgaatt acaagcaacc tttcaacggc caccggatac cctcattgca ttcttccttc
     1081 gacattatca gggcagccca ggccaactac ctgcccgaca ggataatgat caccgtccat
     1141 ccacagcgct ggacaaacaa gccgcttcca tggcttcgcg aactgatttg gcaaaatgtg
     1201 aagaataccg ggaaatgggc tctggcaagg gtcaggggca gatgaaaatg tcatgattta
     1261 cgtatcatga acagcaagtt ttttcaatcc ttaaacaggg cccaggaatc tgcaggatgt
     1321 atcctgcttt ttatcttttc cctgcaatcc tgatgatccc ccagcccgcc aatatcccgg
     1381 ccatgttcgc cacccagtcg aatatgttga atgcccgtgc aggcacccat atctgtgcca
     1441 cttccgtaac agtcgccagg atcattacca gaaggatgaa ccgttttagc ggatttttat
     1501 cgaacaatat caggcctttc ctttcaccaa acagataaaa catgcatatc aggaaataaa
     1561 cggtaaaatg cagcaggtga tccgtcctta tggaaaatgc ttccggacca agccttattt
     1621 tttcaacccg gaaacttatc gggagaaaag ttgcgatcag cacggcaaaa taacctgtcc
     1681 agaagacata cctgtaaagc ctgagatact tttccatcac tgttttttgc agggacaaag
     1741 ataaatcttc ttttgtaatg aggaacatag gtgcagattg catcaggcat gttgcagggc
     1801 actaaatcct gaactttgat tcctgaagtt ctttgccggc cggggcgtcc tttctgcaat
     1861 ccgggataag aacttttatc ttaaatttac ggaaagaatt ctgatcccgg tccttatgcc
     1921 cagtcctgag gaaaaactgc tgctttccat ttgccgtctc gagttcggtg aaaatgaaac
     1981 aggcaacctg aaattgcttg cagacgaaat aaaagactgg atgcaatttg caaacctggc
     2041 aaataaacac ggcataattg cacttattgc ctataatgtc agggcaacaa atcttgccaa
     2101 cctgataccg gataatgtaa accggctgct gaatgacggc ctgatgcaaa gcatcctgag
     2161 aaatacatgg ctcaccgaaa ggtggaaaga agttaaccga atactgacag atgccggaat
     2221 aaagcatgtg cttctgaagg gcatggccct ggaacatacg gtgtacggat cacggggact
     2281 tcggcagatg acggataacg acatacttat cagcagggaa gacggggtaa aggcctggga
     2341 acttctgcag gaacacgggt tcgtaacaca gccggtcaag tcgcccctgc atcgtaaaat
     2401 aatagcagat atcggaaaac atctgccgac cctggttaaa gacggatacg cacttgagat
     2461 acatctgagg ctgtcaggtt ttggtaaagc ggcttcagac ccagccaata tcatggataa
     2521 tgccctcccg attaaaattg acggcataac agcttttatt cctgatgacg cgacccacat
     2581 caagtacctg gcagaacaca gtatttatca ccagaccatt tccggaccgg agctgaagct
     2641 tttcctggat atttcgcttt tgaagaagga tggttatgaa agcatcaatc cccggatatt
     2701 tactgatcct tcggcatata aaaaccccgg ttacaaaaag aagatctacc gtgaacaggt
     2761 tttaggcctc tcccatggca aaaggctgag gttcctggca ggggatatct ttccttctcc
     2821 tgtctggatg aggcaaagat ataagtgcag cctggcaggc gtgctgatcc gttatccggc
     2881 aagactctgg aaactgacat ggctgttgtt ctgacggaaa cttcaatgac cagaggtaag
     2941 aaacggatcg gacattataa tataacctgt tgaaaaccgt ttttatttgg ttgataacta
     3001 aaaatctatg gtttgcttaa acaggctaat ttcccatgtt ggaattactg ccggaatgtt
     3061 aacataattg tcataccgaa ggagtggatt atttaatacc gtcatactga ttttttaata
     3121 accaaaatct catatattat gaaaaatgca attgtaattt tacgtaattt gctcataata
     3181 actgccgtgg caggactttt tgcatgtgag ggccctgatg gtcccatggg tccggccgga
     3241 ccgaaaggtg acattggtcc tgctggtcct gctggtccga cgggtgagac cggtcaggca
     3301 gggaaagacg gtaacgccaa tgttacggta atcagcctgc tcagcaagga tatcacatgg
     3361 ctggaagggg aatacctgcg ccgtcccgct aacttctatt caattgaaga tgccccggtg
     3421 gatgacgata tcatcgacca cggcgttgta cttggatact gctctgtcta tgacaactgg
     3481 caaatccttc cgcttacatg ggagaacagt gacggcaccg aaaggcagta tatcctgcac
     3541 agctattccc cgaataccat taccctgtat gcctatcaga cttcaggagt catggagccc
     3601 gatcccttaa ttataaccga ataccgcttc atgctaataa cggataaaac agtaaccggg
     3661 acaaaaggag ttccagctga aaaaaatatc ctgatgaagc ttgaaaaggc aggagtgaac
     3721 attaataatt accacgaagt gctgaactat ttcgggctca ggcattagcg gcaggccgga
     3781 tgaatgtggt caataaaccg gatgtgcaga ctggaaaaaa tctgtaaaaa agataaccag
     3841 actacacacc ctgatcctgt atactcatta ttttttttga cggacgaaaa ctatttttac
     3901 aggattaata acgcatttcc tgcatattag ttttatattt actgtttgag aaaacattga
     3961 cagcaaaaca aacaacaccg tcagattgtt aaaaagtacg actgcgaaac aggtgaactt
     4021 gtccggtcag gtcaacgtat accggaatac tgaaagggaa ccagcatatc cgcacttcaa
     4081 aaaacagaag aaatgaaaaa cctattctac aaatgcctgc tgttcagcat aaccatatta
     4141 atgactgcct tttattctga tccagtttcg ggacaggtaa ccgcgattaa ggactggacg
     4201 agtgtttata gcggcaattc aagctccacc cagaatatta actacccggt tcctgccggt
     4261 accaatgcca accggctgat ggttgtggca atagcgtcgg aaattaccgc agcagtcacc
     4321 cagaatgtga ccctgaccta cggagggcag cccctgacac tggctgtagg tgactttggc
     4381 acctcctctg cttttcatac aggattttac tatctgaatg aagccggact tgatgcagca
     4441 ggaataaaca ccacattatc ggtcatggtt acgggcggca ctgtaagact gacagatgta
     4501 tgggcagctg tctttgatca tgttaaccaa accactccta taaccgatgc aaggaaccat
     4561 aacagcagag gtatttacag tacaagtttt tctttcaatc ctcctttgca ggttaactca
     4621 ttcaacaagg ctttagaagt ggtcggcatc tttaattccg gcctcaacca gttccggaca
     4681 gtgacttatg ccgatcagtg ggaaatgatc tttgaatcaa ccagatcgga cggggagggc
     4741 cttggtagtt attacagcat aaaaaactgc ctggccggca gaaacacacc tgtatcaaac
     4801 ctaactgacg ccgctgaaat atcatttgat aaaagcgcac gcgtttccat aaccgggata
     4861 agcctgaact acactccccc tccccctccg acgatccagg catcaaatgt caccttcagc
     4921 aacataacct cgtcttcttt caccgttaac tggaccaacg ggaacggggc aaaccggctt
     4981 gtcattgtaa gggaaggtag cccggtagat gctgctcctg ctgacggaac aacatatact
     5041 gcaagcaata actggacagg agggcaggaa atatctccgg gaaattatgt ggtttataac
     5101 ggaactgctg acagcgtcaa tgttgtaaac cttgacgggg gcaaaacata ccatgttgcg
     5161 gtgtttgaat tcagcggccc tcccggaatg gaagactatc ttaccgttcc agggcccgca
     5221 agggggaacg tcactacatt gcctgaaacg gcagtcaatg gtgattaccg ctccgcagcg
     5281 agcggcaact ggggatcacc ggcctcatgg caggtttacc atgacggagc atggacaact
     5341 gcaagctcgg ctccctcaag ttccgacggt tttataacga taatatccgg ccacacgata
     5401 accgttgctg aaaacgtgac agccgaccag gtggttatta atgccggagg aaccctgcat
     5461 gtgaacagcg gtattacgct gaccatcgct gacaatgcag gaccgggaaa cgatacgatt
     5521 gattgttcag tgagcgggac cctgtatgtt gccggaactt tggttccgaa cggggaattt
     5581 gctttcaaca ggggttccgt ttatcatcat gcaacagacg ggggtaccat tccagcagcg
     5641 gaatgggata cagagtccca atgccttgta accgggataa cgaacactgc ccctgccggc
     5701 ctgggacaaa gcttcggtca ctttacctgg aactgcgcct cccagagctc ggcgcttacc
     5761 gtatcattta acaatgatat taccataaaa ggcgatttta ccatcacctc caccgggacc
     5821 ggtaagcttg ccctgacaag caacccgccg caggtatcga tgacggtggc aggaaactac
     5881 acacagacag gcgggacctt tatattgaat aattcagcct cttcaacggt aaccgatagc
     5941 ctgtacgttg cgggcaattt cctgttcaca gggggcacca ttaccgaaac atcaaacacg
     6001 ggacgcgggg aaatcatctt taacggcagc ggagccatgc aaatgtacac atcgggaggg
     6061 acattctcag agaccatcga tttcctggtt gagcccggag catatctcca gctcggaacc
     6121 ggtgcaacac ccggctatat cctctcaagc aaaggcaaat ttaccctttc acccggggca
     6181 accctgggaa taacttcccc gtacggcatt acaaaaacaa gcactacaaa tcccaatgga
     6241 ggcaatatcc gtgt
//
LOCUS       DAOOYG010000149         6218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_14483_length_6218_cov_6.048191, whole genome shotgun sequence.
ACCESSION   DAOOYG010000149 DAOOYG010000000
VERSION     DAOOYG010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14483_length_6218_cov_6.048191"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PK288_10335"
     CDS             complement(<1..1293)
                     /locus_tag="PK288_10335"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPM19366.1"
                     /translation="MKKKYFYIQFLLSLAILIPPVSTLAQRGQQANVVTIESVVTDVS
                     GNPVRATIYGNEGMVVVKTDAQGKFTITVPSQTDLFIEARDYESRVFKAGEIQTLKAF
                     QLTRSEYRFGEKDDVNVAFGKVKKGNLVGAISVMEPDEIVEFDNYQWLSNALEGRITG
                     MRGSSNIRGIGTPMFIVDGLPRDIMNLSMNEVDQVTILKDVNSSVLFGSRAVNGVVLI
                     TTKRGEPYKKDLKVSGYYGIDLPLELPNYLPSAQYMELYNEARLNDGLAVQYDNATIE
                     NYRTGDPYRYPNIDYYSSEYLKPLKQEFMATTELSGGNEVATYYANVGWSQSGSLLNF
                     GEGKNGAENMFNIRGNVDLKINDFVKSAIDAVAVFANSKSQTGDFWGNASTYRPNLFA
                     PLLPIRLVSDDLSSIVESRKTDVNGQFLVGGTKAYQTNP"
     gene            complement(1326..3128)
                     /locus_tag="PK288_10340"
     CDS             complement(1326..3128)
                     /locus_tag="PK288_10340"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM19367.1"
                     /translation="MKKINIKLKLNFAAGAILGLLLIASCENYLDKAPEATITEKDAY
                     GNFRSFQGFIEELYNCIPDPNKCGAWNQYLFADEVLNNNLYAFDQGNYWSQGSYMYGT
                     SVNTDNNSRTKRLWPLAWYAIRKANLALAKLPLLADATQEEKDLIRGQALFFRGFMYF
                     ELMKWWGGLPYIDTVLSPTQDLSFPRLTYKETALKAAADFRAAANLLPMNWDDIDAGQ
                     QTLGNNDDRINKLHALGYLGKNYLYAASPMMNESSGGPAAFDTELCKLAAAAFAEAIK
                     LNDQTGRYQLQSWATWTDNFWVWSPSNRTMSGGTEVIMNCHVFDAGRVRWSTVGQTCP
                     VQFGGNTSTVEVPTWNYIKNYAMANGLPISDPLSGYDENDPWANREPRFYSDIAVDGD
                     KMVASAASGPDQFIQTYTGGSHRGTANGTVTGFYYKRYSPIGCNQWDNKWSAFQAYVP
                     FLRLADIYLMYAEAVLQGYGSPSSSDPGLPALTAEGALNIIRNRAQLPNITVAYTASK
                     EIFMEEIIRERAVELAYEGARFQDLRRWNLNTLPKYKIKTALDFDRDPDTGKPVNIKE
                     RTVLVRVAEKKHNWLPFQVSFTKQYEGFPQNPGW"
     gene            complement(3140..>6218)
                     /locus_tag="PK288_10345"
     CDS             complement(3140..>6218)
                     /locus_tag="PK288_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM19368.1"
                     /translation="VIGVTVLVKGTTLGALTDAAGRYTLPNVPPNSVLSFTFIGMTPQ
                     EIPLQSGQTKLDVVLQEATLALEEVVVIGYGTQKKESVVGAISQTTSEQLQRTGNVTD
                     FKQALTGQLPGVVTLTASGEPGGTGRGYSATNIFIRGMNTWNGGQPLILVDGVERSMD
                     NLDVNEVENISILKDASATAVFGVKGANGVILINTKRGSSGTGKPKLSFTYNATGKML
                     SKLPEMMESYSAIMIRNEAIEREVVLNEPSWLDYIPGELALRYKKPQLPEYVEIYPNV
                     DWEEAMFRDVGMTHRAALDIRGGTKVVSYFGSLSYLNEGDMFKKYDNHKGYEPSFAFN
                     RFNFRSNLDFKVTSSTTLKVNLSGYFSLKNTNYSNEGSTSGADQWMWNGVYTMPPDLY
                     LPQYSDGRWGWSSWSTKVNPVAAVYNLGVRQTRTTELNSDYHLEQNLDFITKGLSAKA
                     SLFYDNQIKSQGGLYDAANHIRASEGNTPEKTIDASLYEGPDQDPGEYTINWPITGPN
                     QFDWNYNPWYIIGEVIGAAMWESTIPVTRRMMYQFQLNYARKFGRHNVTAMGLMKREE
                     YARGSMFKNYREDWVFRTTYDYNTKYFFEFNGAYNGSEQFGPGYRFDFFPSLAVGWNV
                     ANEKFWNIQQINRLKVRYSVGLVGDDNIAGGDRWLYASQLSYGGTGRLAAQTNVMSPY
                     VWYREAVVGNPDIHWEKATKNNLGIEMGLFRDLFSITYDFFTESRTDILLEGSARSIP
                     PFFGTTPPGANVGEVKSHGHELEVKFDKRTNTDWRFWTNLSLARNINEIINRDDPALL
                     DAHQKRAGFAIGQTKTTVRTGFYNNWDEIYGSVPMETNDASKIPGFYNILDFNADGVI
                     KSSDDAVPWGFSEIPQNTCNIQSGIGYKGLSLMIQLYGVNNASRSFGMDNLPASTNVV
                     FGHVADYWSEDNLNATSFMPRWKTAGENIGDYYIYDSSYWRLKTAEIAYTFDSQWIKK
                     AGLASLKIFLNGNNLLFWSDLPDDREVAWSGGSASGGAYPTVKRINLGVDLTF"
BASE COUNT         1599 a         1531 c         1279 g         1809 t
ORIGIN      
        1 gggatttgtc tggtatgctt ttgtacctcc tacaagaaat tggccgttca catcagtctt
       61 tcgggattca acaatactgc ttagatcatc tgaaacaagt cttatcggaa gcaggggagc
      121 aaaaaggtta ggcctgtagg tcgaagcatt tccccagaaa tcaccggtct ggcttttcga
      181 attagcgaaa acggctacgg catcaattgc gctttttaca aagtcattaa ttttaaggtc
      241 tacgttacct ctgatattaa acatgttctc agcaccgttc ttaccttctc cgaagttaag
      301 aaggcttccc gactgagacc agccgacatt ggcataatat gttgcaacct cattaccacc
      361 cgataactct gttgttgcca tgaactcctg tttaagaggt ttcagatatt cggaagaata
      421 ataatcaata ttcggatagc ggtagggatc acctgtcctg taattctcaa tggttgcgtt
      481 atcatactga acagcaagac cgtcattcaa cctagcttca ttatacaatt ccatgtattg
      541 tgcagatgga aggtagttgg gaagctcgag tggcagatcg attccgtagt atccgctgac
      601 tttgagatcc ttcttataag gttcacctct ctttgtggta atcagtacaa caccattaac
      661 tgccctgctg ccaaatagca cagatgagtt cacatcttta agtattgtaa cctggtcaac
      721 ttcattcatc gatagattca tgatatcacg cggcagacca tcaacgataa acatcggagt
      781 tccgattccc ctgatgttgg aggaacccct catcccggta atacgcccct ccagggcatt
      841 tgaaagccac tggtaattat cgaattcaac aatttcatcc ggttccataa cgctgattgc
      901 accaaccagg ttgcctttct taaccttacc gaaagcaaca ttaacatcat ctttttcacc
      961 aaaccggtat tcggaccggg taagctggaa cgctttaaga gtctggatct caccagcttt
     1021 gaagaccctg gattcataat ctctggcctc aataaacaga tcagtctgtg atggaaccgt
     1081 aatagtgaat ttgccctgtg catcagtttt caccacaacc ataccttcat taccataaat
     1141 tgtagcgcga acaggattcc ctgacacatc agtaaccact gattcaattg ttacaacatt
     1201 tgcctgttgt cccctctgtg ccagggttga cactggcggt atcagaattg caaggctcag
     1261 caggaattgt atataaaaat attttttctt catttttcag gaatttaagt gttataactt
     1321 ctgacctacc agcccggatt ttgcggaaac ccttcatact gtttggtgaa gctgacctgg
     1381 aacggaagcc agttgtgttt cttttcagcg accctgacta atacagttct ctcctttatg
     1441 ttaactggtt tcccggtatc tggatcacga tcaaaatcaa gtgcagtctt tatcttatat
     1501 ttaggcaggg tattaaggtt ccagcgccgt aaatcctgaa aacgggcacc ttcataagca
     1561 agttcaacag cacgttcgcg gatgatctcc tccatgaata tctcctttga ggcagtgtat
     1621 gctactgtaa tgtttggcaa ctgagcccgg ttccttataa tattaagggc tccttctgcg
     1681 gtaagagcag gcaggccggg atcgctgctt gaaggcgatc cgtaaccctg aagaactgct
     1741 tcagcataca taaggtaaat atctgcaagg cggaggaacg gtacatatgc ctggaaggca
     1801 ctccatttgt tatcccactg gttacatccg attggagaat accttttata ataaaaccct
     1861 gtcacagtcc cgtttgcagt accgcgatga ctaccaccgg tataagtctg aataaactga
     1921 tccggtcctg aagccgcact tgcaaccatt ttgtcaccgt caacagcaat atcactatag
     1981 aaacgtggtt ccctgtttgc ccagggatcg ttctcatcgt atcctgataa cggatcacta
     2041 atgggtaatc catttgccat tgcgtagttc ttgatataat tccaggttgg aacctcaact
     2101 gtactggtgt ttcctccaaa ctgaacggga caggtctgtc caacagttga ccatcgtacc
     2161 cttcccgcat caaaaacatg gcagttcata atcacttcag ttcctcccga cattgtcctg
     2221 ttactagggg accataccca gaaattatcg gtccacgttg cccaagactg taactgatat
     2281 cttcctgtct ggtcattaag tttaatagcc tccgcaaaag ccgctgctgc cagcttgcag
     2341 agttcagtgt caaatgctgc cggtccacct gatgattcat tcatcatggg acttgcagca
     2401 tacagatagt tttttccaag gtatcccaga gcatgcagtt tattaatcct gtcatcattg
     2461 tttccaagtg tctgctgacc agcatcaatg tcatcccagt tcattggcag caggtttgct
     2521 gcggctctga aatctgctgc tgcctttaag gcggtttcct tataggttaa ccgggggaaa
     2581 ctcaaatcct gggtgggaga taatacagta tcgatatagg gcagccctcc ccaccatttc
     2641 atcagttcaa aatacataaa tcctctgaaa aacaaagcct gccctcttat cagatccttt
     2701 tcttcctgag ttgcatctgc taataacggt agcttggcta atgcaagatt agcttttctg
     2761 attgcatacc atgccagcgg ccataatctc ttcgtacgcg aattgttatc cgtattaaca
     2821 ctcgttccgt acatgtaact gccctggctc cagtaattac cctggtcgaa tgcatataga
     2881 ttattattaa gaacctcatc agcaaagaga tactggttcc atgccccgca tttattagga
     2941 tccggtatac aattgtataa ttcctcgatg aaaccctgaa aacttctgaa attaccataa
     3001 gcgtcctttt cggtaattgt cgcttccggt gctttatcca ggtagttttc acatgacgct
     3061 attaacagta acccaaggat ggcaccagct gcgaaattca gcttgagttt tatattaatt
     3121 tttttcattg tcgctttagt taaaatgtta agtcaacacc gagattaata cgcttcactg
     3181 ttgggtaggc tcctcctgat gcactaccac ctgaccatgc aacttctctg tcgtcaggaa
     3241 gatctgacca gaagagcagg ttgtttccgt tcaggaatat tttaagtgac gccagaccgg
     3301 ctttctttat ccactggctg tcgaacgtat aggcgatctc ggcggttttt aacctccagt
     3361 aagatgagtc gtatatgtaa taatctccga tgttctcacc agcagttttc caccttggca
     3421 tgaaggaggt tgcattcaga ttgtcttctg accagtagtc agcaacatga ccaaatacaa
     3481 cgtttgtgct ggctggcaga ttatccatac cgaagcttcg ggaagcattg ttaaccccat
     3541 acaactgaat catcaggctt aaacctttat agccgattcc agactgtata ttacaggtat
     3601 tctgaggaat ctccgagaaa ccccatggaa cagcatcgtc gcttgatttg ataacaccgt
     3661 cggcattgaa atccagaata ttgtagaacc cgggtatctt tgatgcatca tttgtctcca
     3721 tcggaacact cccgtaaatt tcatcccagt tgttatagaa tccggttctg actgtggttt
     3781 ttgtctgtcc gatagcaaat ccggctctct tctgatgagc atccagcagt gccggatcat
     3841 ctctgtttat aatttcattg atatttctgg ccaatgaaag gttggtccag aacctccagt
     3901 cggtgtttgt tcttttatca aactttactt caagttcgtg cccatgagac ttcacttctc
     3961 cgacattagc tccgggaggg gttgtaccaa aaaagggagg tatgctcctg gcgcttcctt
     4021 ccagcaagat atcagtcctg gattccgtga agaaatcata ggtgatggaa aagagatccc
     4081 tgaagagtcc catttcaatc ccgagattat tctttgtcgc cttctcccaa tgaatgtcag
     4141 gattgcccac aactgcttcc ctgtaccaga catacgggct cataacatta gtctgagctg
     4201 caagtcttcc ggttccgccg taagatagct gtgaagcata caaccatctg tcacctcctg
     4261 caatattatc atcaccaacc agcccgacac tgtatcgaac tttcaatcta ttgatctgct
     4321 gaatgttcca gaatttttcg ttcgccacat tccagccaac cgccagtgac gggaagaaat
     4381 cgaatctgta gccgggaccg aactgttccg aaccattata agcaccattg aattcgaaga
     4441 aatattttgt attataatca taggttgtcc tgaaaaccca gtcttcacgg tagttcttaa
     4501 acatacttcc cctggcatat tcttcacgct tcatcagtcc catggctgta acgttatgcc
     4561 ttccgaattt ccgggcataa ttaagctgga actgatacat catccgtctg gtaacaggaa
     4621 tggtgctttc ccacattgcg gctccaataa cttccccaat gatataccat gggttatagt
     4681 tccagtcaaa ctggttggga cccgtgatcg gccagtttat agtatattca ccgggatcct
     4741 ggtcaggacc ttcgtaaagt gaagcatcga tggtcttttc cggagtattc ccctcgcttg
     4801 cccggatatg gtttgcagca tcatagagac caccttgcga ttttatctgg ttatcataaa
     4861 acaacgacgc cttggcactc aggcctttgg tgatgaaatc aaggttctgt tccaggtggt
     4921 aatccgagtt cagttctgta gttctggtct gcctgacacc cagattatac actgcggcaa
     4981 caggattcac ctttgtcgac catgaggacc aaccccatct gccatctgaa tactgaggca
     5041 ggtacaggtc cggaggcatg gtgtaaacac cattccacat ccattggtcg gcaccgcttg
     5101 tacttccctc attgctgtaa ttcgtattct tcagactgaa ataacccgac aggtttactt
     5161 tcagggtagt tgatgaggta accttgaagt caaggttact tctgaagttg aacctgttaa
     5221 aggcaaaact tggttcgtaa cccttgtggt tatcatattt tttaaacata tcaccctcgt
     5281 tcaggtaaga gagtgaaccg aaataggaga ccaccttggt tccacccctg atatccagcg
     5341 ctgcccggtg tgtcatacca acatccctga acatggcttc ctcccagtca acattcggat
     5401 atatctcgac atattccggt aactgaggtt ttttatatct cagagcaagt tcaccgggaa
     5461 tataatcgag ccatgacggt tcattaagta ccacttcacg ctcaattgcc tcgtttctga
     5521 tcataattgc gctgtatgac tccatcattt cgggaagttt agacagcatc ttccctgttg
     5581 cattgtatgt gaaagaaagc tttggttttc ccgtacctga agagccgcgt ttggtgttga
     5641 taagaataac cccgtttgct cccttaactc caaacacagc ggtggctgaa gcatccttca
     5701 gaatggatat attctcaacc tcgtttacat cgaggttatc catactgcgt tcaactccat
     5761 caacaaggat cagaggctgt cccccgttcc atgtattcat accgcggatg aagatgttgg
     5821 tagcactgta acctctgccg gttcctcctg gttctccgga agcggtaagg gtaactacac
     5881 ccggcagctg ccctgtcaaa gcctgcttga agtcagtaac gtttccggtc cgctgaagct
     5941 gttctgaggt tgtttgagaa atagctccaa caacactctc cttcttctgt gtaccataac
     6001 cgataaccac tacttcctcg agagccaggg ttgcttcctg aagaaccaca tcaagctttg
     6061 tctgtccgga ctgtagtggt atctcctggg gtgtcattcc gatgaaggtg aacgacaata
     6121 ctgaattggg tggcacgttt ggaagagtat atcttcctgc tgcgtcagta agtgcaccaa
     6181 gggttgtccc ttttacaagg accgtaactc cgataacc
//
LOCUS       DAOOYG010000150         6153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_14693_length_6153_cov_5.047721, whole genome shotgun sequence.
ACCESSION   DAOOYG010000150 DAOOYG010000000
VERSION     DAOOYG010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14693_length_6153_cov_5.047721"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..1401)
                     /gene="dprA"
                     /locus_tag="PK288_10350"
     CDS             complement(292..1401)
                     /gene="dprA"
                     /locus_tag="PK288_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPM19369.1"
                     /translation="MQSEIPLKHKIALGLIPGVGDIYARRLVSSLGSVEAVFEESYRN
                     LIKIPGIGTSLARSISDRSYMEIAEKEAEYVTRNNIRTFFYLDADYPFRLRQCDDSPV
                     VFFFKGNCNLDESRILGVVGTRSATPRGRDICGKIIGELAASHPELIIVSGLAYGIDI
                     TAHKAALSYNLATVGVLGHGFRTIYPSVHKPTAEAMQKKGGLISDFVSGELPERNNFI
                     KRNRIIAGLSDAILVIESGIKGGALITADIAASYNRDVLAVPGRPEDQWSAGCNALIK
                     NNKAALVESADDIDYFLGWRAAKSKSPVQPSLFPELDENEKLIYELISGEGESDIDHI
                     CRTLDIPVFRLSPILLQMEFRGLIRCCPGNIYRLA"
     gene            complement(1426..2763)
                     /gene="gdhA"
                     /locus_tag="PK288_10355"
     CDS             complement(1426..2763)
                     /gene="gdhA"
                     /locus_tag="PK288_10355"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPM19370.1"
                     /translation="MNVDRILNNLEKKHPGETEYLQAVREVLASIEEVYNENPQFESA
                     RIIERLVEPDRIIIFKVPWIDDEGDIRVNLGYRIQFNCAIGPYKGGLRFHSSVNESIL
                     KFLGFEQVFKNALTTLPMGGAKGGADFEPKGKSNNEVMRFCQSFMLELWKYIGPETDV
                     PAGDIGVGGREIGYLYGMYKKLKGAHTGVLTGKGSNWGGSFIRPEATGYGASYFAQEM
                     LKSRKETLAGKTVSVSGFGNVAWGTVLKATELGAKVVTISGPDGYIYDPEGISGKKID
                     YMLELRASNQDIVRPYSYVFEGAEFHEGKRPWEVKVDIAFPCATQNELNADNARMLIS
                     NGVKMVAEVSNMGCTPDAVEEFQKHGILFAPGKAVNAGGVATSGLEMTQNAMKMKWNI
                     EEVDRRLREIMINIHEQCVRHGKRPDGSVDYVKGANIAGFLKVAYSMLDMGVI"
     gene            complement(2963..3469)
                     /locus_tag="PK288_10360"
     CDS             complement(2963..3469)
                     /locus_tag="PK288_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19371.1"
                     /translation="MTLDNSKTIISLRIKLFAATVIFLAFIVLTYVAKKINYPLLGMT
                     DTAWTVILASCYAFLMFLPMYLNYQYVYFSDEGENIVFRYFNAGIVGGRKNSVEINKK
                     AFSGYQIEKKLFGLRTSIILYTQLKQGIAKYPPIHISALKRVEREKIMKILNAYAPRI
                     KDGTEDDT"
     gene            complement(3629..4069)
                     /locus_tag="PK288_10365"
     CDS             complement(3629..4069)
                     /locus_tag="PK288_10365"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPM19372.1"
                     /translation="MTESRDEQFMRAAISEARKAYEKQEVPIGAVIVCNDMIIARAHN
                     LTETLRDPTAHAEMQAITAAAEWLGGKYLTDCTIYVTVEPCAMCAGAIGWSQARRLVF
                     GAADNKKGFSLISNRLLHQATDVEHGVLANECGELMKKFFKTRR"
     gene            4207..5961
                     /gene="aspS"
                     /locus_tag="PK288_10370"
     CDS             4207..5961
                     /gene="aspS"
                     /locus_tag="PK288_10370"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPM19373.1"
                     /translation="MYRTNTCGELNIRDAGKEVTLCGWVQRSRDLGGMTFVDLRDRYG
                     ITQLVFNMDTNRELCDKARKLGREFVVRAKGKVRERSNKNPRLSTGDIEIEVSELIIL
                     NKSEHPPFTIEEETDGGEELRMKYRYLDLRRPTVKNNILLRHKVAREVRNYMDSAGFI
                     DIETPVLIKSTPEGARDFVVPSRMQPGTFYALPQSPQTLKQLLMVAGFDKYYQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVGRDEILDTFEGLAKHLFRQVKQIEFNGSFPRLTYR
                     EAIENYGSDKPDMRFEMKIRELTHLVRGKGFSVFDNSMYTGGICVPGCADYSRKLLDE
                     LTDFVRQPGIGAKGLVYLKYGSDGSLKSSADKFYTSQDLEKWAQAVGARPGDLVLILS
                     GSAEQTLAALSELRLEMGDRLNLRPKDKFAPLWVIDFPLLEWDDETNRFYAMHHPFTS
                     PHPSDIHLMETDPGKVRANSYDLVINGVEIGGGSVRIHDAAIQKLMFKTLGFTETEAE
                     EQFGFLMNAFRYGAPPHGGIAFGLDRWVALLGGQESIRDFIAFPKNNSGRDIMLDSPS
                     EISGKQLDELKLIVRKEK"
     gene            complement(5974..>6153)
                     /locus_tag="PK288_10375"
     CDS             complement(5974..>6153)
                     /locus_tag="PK288_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HPM19374.1"
                     /translation="KTFTVLAEGRSKRSPDFLSGRTSQNKVVVFPGAGISPGDYVNVT
                     IERSTSATLIGKAVK"
BASE COUNT         1575 a         1494 c         1373 g         1711 t
ORIGIN      
        1 ttaactatct tattgacatt caaacaatta tattaatatt taacgaagta ttgtaaggct
       61 ttatgattat ttattgtttt gggttgcttg tcacgcccaa tttcatcctc cgtatcagaa
      121 tacctcaccc cctcttatcc cccgctccca tgagagcggg ggacgatagt tgcctgcaag
      181 gttataagtc tttgtttgtt tgagttggtc tataattatg gtgttcttaa agtccctccc
      241 cccaggggga gggatttagg gagaggcatt ctccccccgg aaggatagaa ctcaagccag
      301 ccggtaaata tttccgggac agcatcttat cagacccctg aactccatct gcagcaggat
      361 cggggagagt cggaaaacag gaatatccaa tgtccggcag atatggtcaa tgtctgattc
      421 gccttctcct gaaatcagtt cataaatcag tttttcattc tcatcgagtt cgggaaagag
      481 tgaaggctga acgggagatt ttgatttcgc cgcccgccat ccgagaaaat aatcaatatc
      541 gtcggccgat tctacaagtg cagccttatt gttcttaatc agggcattgc agccggccga
      601 ccattgatct tcaggtctgc cggggacagc aagcacatct ctgttgtatg aggctgcaat
      661 gtctgccgta attagggctc ctccctttat tcctgattca atgacaagta ttgcatccga
      721 taatcccgct attatcctgt ttcttttgat aaaattgttc cgctcgggga gctccccgga
      781 tacgaaatca gatatcagtc cgcccttttt ttgcatggct tcagccgtag gtttgtgcac
      841 tgatgggtag attgttcgaa aaccatgacc cagaaccccg acggttgcaa gattgtatga
      901 aagggctgcc ttgtgcgccg taatgtcgat tccataggca agcccgctga cgatgatcag
      961 ttcaggatgc gaagccgcca gttcgccgat tattttaccg catatatccc tgcctctcgg
     1021 ggtggcactt ctcgttccga ccacgcccag aatccttgat tcatcaaggt tgcagttgcc
     1081 cttgaaaaaa aataccacgg gtgaatcatc gcattgcctt aacctgaagg gataatctgc
     1141 atcaagatag aagaatgtcc tgatattgtt cctcgtgaca tattctgctt ctttttcggc
     1201 aatctccata tatgaacggt cactgatcga ccgggcgagg ctggtgccga taccgggaat
     1261 ttttataagg ttcctgtatg attcttcaaa aactgcttca acgcttccca gactggagac
     1321 cagcctcctg gcgtaaatat ctcccactcc cgggatcagc cccagggcaa tcttatgctt
     1381 cagcggaatt tccgattgca tggtatggga atagcaccag atgtatcaga taacacccat
     1441 gtcaagcatt gaataggcta ccttcaggaa tcccgcgata ttggcgcctt ttacataatc
     1501 aaccgagccg tcgggacgct tgccatgcct gacgcattgc tcatgaatgt ttatcatgat
     1561 ctcacgtaaa cgccgatcca cttcctctat gttccatttc attttcatgg cgttttgtgt
     1621 catctcaagt cccgatgttg caacgcctcc agcattcaca gcctttccgg gagcaaacag
     1681 aatgccatgt ttctggaact cttcaaccgc atccggggtg catcccatgt ttgatacttc
     1741 ggcaaccatc ttcaccccgt tactgataag cattcttgca ttatcagcgt tgagctcgtt
     1801 ttgggtggca cagggaaatg ctatatccac ctttacttcc cacggacgct tgccctcgtg
     1861 gaattcagcc ccttcgaaaa catatgaata gggccttacg atatcctgat ttgatgctct
     1921 cagttcgagc atatagtcaa ttttcttgcc ggaaatccct tcaggatcgt agatgtatcc
     1981 atcgggccct gatatagtga caactttggc tccaagctca gtggctttaa gtaccgttcc
     2041 ccatgcaacg ttaccgaatc ccgatacaga aaccgtcttc ccggcaagtg tttctttcct
     2101 gcttttaagc atctcctgtg caaaatagga tgccccgtaa ccggtagctt caggtctgat
     2161 gaaagagcct ccccagttgc tgcccttgcc ggtgagaaca ccggtatggg cacctttgag
     2221 ctttttgtac atgccgtaaa ggtacccgat ttcccttcct ccaacaccga tatcaccggc
     2281 aggaacatct gtttcaggac caatgtattt ccaaagttcc agcatgaacg actggcagaa
     2341 acgcataacc tcattgttcg acttgccttt cggctcaaaa tcggctccgc cctttgcacc
     2401 acccatggga agagttgtaa gggcgttctt gaatacctgt tcaaatccaa gaaacttcag
     2461 gattgattca ttaacactcg aatggaaacg caagcctcct ttatatggcc cgatagcaca
     2521 attgaactga atcctgtaac caaggttgac cctgatatct ccttcatcat caatccacgg
     2581 taccttgaaa atgataatac gatccggttc aacaagacgc tcaataatcc tggcagactc
     2641 aaactgggga ttttcattgt atacctcttc gatcgacgca agaacctccc ttaccgcctg
     2701 taaatattca gtctctcccg gatgcttctt ttccagattg ttcaatatcc tatccacatt
     2761 cataatactt ttcttttgag ttaaaattta tgttttttgt attctgtcta ttccttgaac
     2821 ttttacaatt gcacggtctc cggttacatt ataagatcgg gttgtcccat gcttttaaaa
     2881 ttacataata ctccctgcct cattttttcc tctcccttgt cagccaactg tcttgctccg
     2941 tcttattcac agttgagtgt aattaagtgt catcctctgt tccatccttg atcctcgggg
     3001 catatgcatt cagtattttc atgatttttt ccctttcaac cctttttagg gcactgatat
     3061 ggatcggcgg atacttggca ataccctgtt tcagctgggt gtacagaatg atgcttgtgc
     3121 gcaggccgaa gagttttttt tcgatctggt agcctgaaaa ggcttttttg ttaatctcaa
     3181 ccgaattttt tctgcctcca actatcccgg cattgaagta cctgaaaaca atgttttctc
     3241 cttcatctga aaaataaaca tactggtaat tcaggtacat tggaaggaac ataaggaagg
     3301 catagcatga ggccagaatg actgtccatg cggtatcagt cattcccagc aatggataat
     3361 ttatcttttt tgccacatag gtcagaacaa tgaatgcaag aaagataaca gtagctgcga
     3421 ataattttat tctcaggctg ataatagttt tactgttatc cagggtcatt tgcagcgatt
     3481 taccggttta atcccggatt actaatttgt atttgcgatc cgttgcagcc cagggtcaaa
     3541 accattgttt gcctgggtgc atttcttcct gatgataagt tgggtctccg ttgcatgaca
     3601 tttattctga attgataatg cactctgttt accttcttgt tttgaaaaat ttcttcataa
     3661 gttcaccgca ttcatttgcc agcactccgt gttcgacatc cgtcgcctga tgaagcaatc
     3721 tgttgctgat caggctgaat cccttcttat tatcggcagc accaaatacc aggcgccgcg
     3781 cctggctcca tcctatggct ccggcgcaca ttgcacatgg ttcaactgtc acataaatcg
     3841 tacaatcagt caggtatttc cctcccagcc attctgctgc agcggtaata gcctgcattt
     3901 cagcatgggc cgtcgggtct cttaatgttt cagtcagatt atgggcgcgg gcaatgatca
     3961 tatcgttgca gacaattaca gcaccaatcg ggacttcctg tttttcatag gccttccggg
     4021 cttcggaaat tgccgccctc ataaattgtt catccctgct ttccgtcata gcctgactga
     4081 aaatgaacaa aattaaaaag aattaccgta tctcccagtg ttgacccgtt taaaaaataa
     4141 gagagcctga aattatcttt ctgcaagctg gtttttttat aattttgctg aacaaaaaat
     4201 ttagtaatgt acaggacaaa cacatgcggg gaactgaata tccgtgatgc cggaaaggag
     4261 gtaaccctgt gcggatgggt tcagcgatca cgggaccttg gcggaatgac atttgtggat
     4321 ctccgcgaca ggtacggaat aacacagctt gtgttcaata tggatacaaa cagggaattg
     4381 tgtgacaagg ccaggaagct gggaagggaa ttcgttgtca gggcaaaagg taaggtcagg
     4441 gagagaagca ataaaaaccc caggctgagt acaggcgata ttgaaattga ggtcagtgag
     4501 ctcattatcc tgaacaaaag cgaacatccg ccttttacca tcgaagagga aaccgacggg
     4561 ggagaagagc tgagaatgaa atatcgttat ctcgatctga gaagacccac cgttaagaac
     4621 aacatactgt tacgccataa ggttgccagg gaggtcagga attacatgga ttcagcaggc
     4681 tttattgata tcgaaacacc tgttctcata aaatcaactc ccgagggtgc ccgcgatttt
     4741 gttgttcctt cgaggatgca gcccggcaca ttttacgccc tgccccagtc gccccagacc
     4801 ctgaagcaac ttcttatggt ggccggattt gataaatatt accagatagt aaaatgtttc
     4861 agggatgaag acctgcgggc cgacaggcag cccgagttca cacagatcga ctgcgaaatg
     4921 tcgtttgttg ggagagatga gattctcgat acattcgagg gccttgcaaa gcatcttttc
     4981 cgacaggtaa agcaaataga attcaacgga tcctttccaa ggctgacata ccgggaagcc
     5041 attgaaaatt acggcagcga caagcctgac atgcgttttg agatgaagat cagggaactg
     5101 acgcatcttg tcaggggaaa gggattttca gtttttgata actcaatgta caccggtggc
     5161 atatgtgtac caggttgtgc agattactca aggaagctgc tcgatgagct tactgatttt
     5221 gtaaggcagc cggggattgg tgcaaaagga cttgtttatc tcaagtacgg cagcgatggc
     5281 tcactgaaat catcagccga taaattctac acatcccagg atctcgagaa atgggctcag
     5341 gctgtcggtg cacggccggg tgatcttgta cttatccttt cagggtctgc ggaacagaca
     5401 ctagctgccc tgtcggaatt acggcttgag atgggcgaca ggctgaacct gagaccgaaa
     5461 gataaattcg caccactgtg ggtcattgat ttcccgcttc tggaatggga cgatgagaca
     5521 aaccgctttt atgccatgca ccatcccttt acctctcccc atccctccga tatccatctt
     5581 atggaaacag acccgggcaa agtgagggca aattcatatg acctggtaat caacggggtg
     5641 gagatcggtg gaggctcagt gaggattcat gacgctgcga tccagaaact tatgtttaaa
     5701 acactaggat tcacggaaac cgaggcggaa gaacagttcg gattcctgat gaacgcattc
     5761 aggtacggcg cgcccccgca tggaggtata gcattcggac tggacaggtg ggtggcattg
     5821 ctgggcggcc aggaatccat acgcgatttt attgcctttc caaaaaacaa ctccggccgg
     5881 gatattatgc tcgattcacc ttcggaaata tcaggaaaac agctggatga acttaagctg
     5941 atcgtccgga aggaaaaatg agaactcacc gtactatttc acggccttgc ctataagcgt
     6001 tgccgaggtg cttctttcaa tcgttacatt aacatagtct cccgggctga tcccggcccc
     6061 gggaaaaaca acaaccttat tctgggaagt acggcccgaa agaaaatcgg gtgacctctt
     6121 tgacctgcct tctgccagca ccgtaaaggt ctt
//
LOCUS       DAOOYG010000151         6143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_14720_length_6143_cov_7.696288, whole genome shotgun sequence.
ACCESSION   DAOOYG010000151 DAOOYG010000000
VERSION     DAOOYG010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14720_length_6143_cov_7.696288"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..2018
                     /locus_tag="PK288_10380"
     CDS             213..2018
                     /locus_tag="PK288_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19375.1"
                     /translation="MKNPDRIPAGILTVFIISMLFTSVVQAQKVKPDGQLDIRSLELN
                     YPVPGIPFYHFRAEIELPRESMIEVEASVDGKALRAVDIRRAADFSDLSRPTIPIRPP
                     SGYNLSQDRTLYRNFQVTGWVRWEPGKDYTVSLSVRMKKDAGSSASDTRLTGTRVVRA
                     PLGAGVYSKDWKGYKSVILSETAGISRKGEPVEVLLAFYADEAQQLERDIRVVGVDPV
                     THALSEVPSQVYDRMEYLKEDDLSPDKDGKPTREAPLWLPTVTARVAFLADVPARTSK
                     VYLIFYNNPQATGKIYLTDLRVQGESPGLQVENSVISVMLHPNSGHLDQIVLKSKPEL
                     PLYHRSETNGAMHWNPDIYPPPRLWTHTADWNPPSNVMSLSGSVIARSDVWGEMRKVP
                     EVEASVRYEFYPGVPYFISSTSMRIRETIQALALRNAEMVIKREMITHAAWYDVVRDS
                     VIIYDIEHIPDFTDLKMEADVPWVLFFSRETGIGFAGIQLSYSNTGLESPLRVLNPFF
                     YITAGPWVYWARGLSHTFLSSNMQQVVPAMKGNIFSERWAYVILETDKTPTPYAPVLE
                     WKKRLTNPLRIDLVEEVDDRVTKVMTEALDDAKSD"
     gene            complement(2401..5778)
                     /locus_tag="PK288_10385"
     CDS             complement(2401..5778)
                     /locus_tag="PK288_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPM19376.1"
                     /translation="MKRPALSFYSGKYKVALLKWLWLIILVIATSGGKDIKLFKPVPA
                     SKSGITFKNVITENVHHNALTHENLYNGGGVAVGDINNDGLDDIFFISNMGYNKLYLN
                     LGNFKFRDITDIAGVAGRPGWKTGVTMVDINGDNLLDIYVCYCTKETVEGRRNELYIN
                     HGDLKFTEEAAKWGIDVPTYSTLGAFFDYDLDGDLDMFLVATNTKVLRGMEFESARVD
                     DDPYAGDKLFRNDGDHFTEVTSQAGIISTGIGYGLGIAIADINKDGWPDIYITNDYIE
                     PDYLYINNGDGTFTNRMTEHVLHNSQSAMGNEINDFNNDTWPDIITADMLSEDNRRLK
                     LLYGQENYLEYALQVMQGFYHSTMRNMLQLNNANGTFSEIGQLACVSNTDWSWAPPFA
                     DFDNDGWKDLFVTNGYFRDYTDRDFLKMKNDYYLEKSKTKEPADTFWLTSFMTSTPVH
                     NYIFKNNKDLTFTDKSKVWGFAKKGFSNGAAYSDLDNDGDLDLIVSNQNETASIFRNM
                     IREHNPKANYISIRLKGPGKNTAGLCSKIYLYTKYGVQYQEQMPSRGYQSCVTTQLHF
                     GLADAEIIDSIRVEWPGGNVNLLTEVGVNQIITIEWHDQNRRFIPEPPPERLFIPVES
                     IVPYEHTEYGSNDFSRQPLLTSMLSPVGPVMATADVNGDGLTDIYVGGTIESPGKLFI
                     QGDDGYFRPSRFFNFPEDLNCTDGDAVFFDADNDGDQDLYIASGGYHEYDVRDRALQD
                     RLYINDGTGKFKKAAGALPPMLTSKSCVRPVDIDKDGDIDLFVGGWVMPGEYPLPQES
                     FILLNDGNGKFVNAAPAMIPALSKGGMITDAAWADINNDSWPDLITVGEFMPIRVFLN
                     DNGKILREATKTWFDVPETGFWNKITVCDFDHDGNPDFIAGNFGLNSQLKASVKEPIE
                     LYFKDFDNNGTIDPIITYYILGQSYPFPSRNEFLTQMPSMRNKFPTYASYADAKLTDI
                     FSPEDLQSATKYTATELRTLVFKNTGSKFVKSLLPMEAQFAPVYAIEILDYNNDGNED
                     IILAGNKNAMCVRLGVMDASYGQLFEGDGKGNFKFVPQPVSGLNMVGDVRSLKVLTIK
                     DHQFLLAGINNIGIVTYFLDNTSSGKTSGVSTRPKQP"
BASE COUNT         1522 a         1367 c         1538 g         1716 t
ORIGIN      
        1 ggatggtagc caccacgtgg atggataatg gttcattcat cgacggcttt cagggtaatc
       61 ttacggagac ggccagaagg gcggtccatc cggaaaatcc tgcatatacc ccgtgggata
      121 ctttcagggc catgtttcag agaatgaatg agcttgaaaa agaaaaataa cgaatcgtcc
      181 cgaatcatcc cgggttttaa attcatttta tcatgaagaa tcccgacaga attccagccg
      241 gtattttaac cgtgtttatt atatcaatgc tctttacttc tgttgtgcag gctcagaagg
      301 ttaagccgga tggtcagctg gatatccggt cgttagagct taactaccct gttccgggta
      361 ttccctttta tcatttccgg gctgagatag agcttccccg ggagtcgatg attgaggtcg
      421 aggcgtcagt tgacgggaag gctctgcgag cagtggatat ccgtcgtgcg gcagattttt
      481 cggatttaag ccggccgaca attcccatcc ggccgccttc gggttacaat ttgtcacagg
      541 accggacgct gtacaggaat ttccaggtta ccgggtgggt aagatgggaa cctgggaagg
      601 attatacggt tagtctttcg gttcggatga agaaggatgc cgggtcttcg gcttcagaca
      661 cgaggcttac cgggaccagg gtggtcaggg ctccgctggg tgcgggggtt tacagcaagg
      721 actggaaggg ctataaatcg gttattctga gtgagaccgc agggataagc aggaagggtg
      781 agccggtgga ggttctgctg gccttttatg cagatgaggc ccagcagctg gagcgtgaca
      841 tacgcgttgt tggggtcgat cctgtgaccc atgcgcttag cgaggttccc tcacaggttt
      901 atgaccggat ggaatacctg aaggaagatg atctcagccc tgacaaggac ggcaaaccga
      961 cacgggaggc tccgctgtgg cttcctacgg taacagcccg tgtagcattt ctggccgatg
     1021 taccggcaag gaccagcaag gtttatctta tattttacaa taatccgcaa gccacgggca
     1081 agatttatct gacagatctt cgcgtgcagg gagaatctcc ggggcttcag gtggaaaaca
     1141 gtgtgatttc ggtcatgctt catcctaatt cgggtcatct ggatcagatc gtacttaaga
     1201 gcaagccgga attgcctctt tatcacagga gcgagactaa cggtgcgatg cactggaacc
     1261 cggatattta ccctccgccc agattatgga ctcatactgc agactggaat cctccgtcga
     1321 atgtcatgag tctttccggg agcgttattg ctcgcagcga tgtatggggg gagatgcgta
     1381 aggttccgga ggtagaagcc tcggtgcggt atgagtttta tccgggtgtt ccctatttca
     1441 taagcagcac cagtatgcgt atcagggaga ctatccaggc tcttgcactc aggaatgcag
     1501 agatggtcat aaaaagggaa atgatcaccc atgcagcctg gtatgacgta gttagggatt
     1561 cggttataat atatgatatt gagcacattc ctgatttcac tgatcttaag atggaggcag
     1621 atgttccgtg ggttcttttt ttcagcaggg agacgggaat aggattcgca gggatccagc
     1681 tgagttactc gaatactggc ctggagtcac cgttgagggt gctgaacccg tttttttata
     1741 tcactgccgg cccctgggtt tactgggccc gtggtctgtc gcacacgttt ctgtcatcga
     1801 acatgcagca ggttgtaccg gccatgaagg ggaacatatt cagtgaaagg tgggcgtatg
     1861 ttatcctgga aacggacaag accccgacgc cttatgcccc ggtcttggag tggaagaaga
     1921 ggctgaccaa tccgctcagg atagatctgg ttgaggaggt tgacgacagg gtgacaaaag
     1981 tcatgactga agccctggac gatgctaaaa gtgattagat tacacgaaag agacgatgag
     2041 atattttttc actgaaaggt ccattatctg atgttgcttt aaaaaacatc ccgggaggta
     2101 agaatattga gaaattttcc ctttaagagg gtatttacgt acttcaatat aaactcctga
     2161 caattattaa ttgccaggtg aatgaagtta gtatgttttc ctgttatttt tctgcaggtt
     2221 accggctccg ggtttgatga atatcccatt gcggcagcct gtattccggc cctgggttta
     2281 taacataccg gtgaagggat taatcaatga tccaggttta actacccccg ttggaaccat
     2341 tgattctttc tttgttatac atatatactt cgggtcgctt gattgtcaca aaagttaggt
     2401 ttacggctgt ttcggccggg ttgaaacacc cgatgttttc cccgaggaag tattgtcaag
     2461 aaaataagtg acaataccga tgttgttgat gcctgcaaga agaaactgat ggtcctttat
     2521 cgtcagcact tttagggacc ttacgtcgcc taccatgttc aatcccgaaa ccggctgggg
     2581 gacaaatttg aaattacctt ttccgtcgcc ttcaaaaagc tggccgtagc ttgcatccat
     2641 aacccccagg cgcacacaca tggcattctt gtttccggcc aggataatat cctcgttccc
     2701 gtcattgttg tagtcaagta tttcaattgc atagacaggg gcaaactggg cctccatcgg
     2761 aagcaggctc tttacgaatt ttgaaccggt gtttttaaac accagtgttc tgagttcagt
     2821 ggctgtatat tttgttgcgg actgaagatc ttcgggggag aatatgtctg tcagcttggc
     2881 atcggcgtag gaagcatagg ttgggaattt gttccgcatt gaaggcatct gggttaggaa
     2941 ttcattcctg ctgggaaatg gatatgactg accaaggata taatatgtaa ttatgggatc
     3001 tatggtgccg ttgttgtcaa agtccttgaa ataaagctca ataggttcct ttacggatgc
     3061 cttgagctgt gaattcagtc cgaagttgcc tgcaataaaa tccgggtttc cgtcgtgatc
     3121 aaaatcgcaa acggtgatct tgttccagaa tccggtttcc ggaacgtcaa accaggtttt
     3181 ggttgcttcc ctgaggatct tcccgttgtc gtttaagaaa acacgtatcg gcataaattc
     3241 cccgaccgtt atcagatcgg gccatgaatc gttgttaatg tcggcccatg cagcatcggt
     3301 aatcattcct ccctttgaaa gagccgggat catcgccggt gcggcattaa caaacttgcc
     3361 attaccgtcg ttcagaagga taaagctttc ctggggaagc ggatattcac ccggcattac
     3421 ccagcctcct acaaacaggt cgatgtcacc gtccttgtca atgtcgacgg gacgcacgca
     3481 cgattttgat gtcagcatcg gggggagggc gccggccgcc tttttgaatt ttcctgtgcc
     3541 gtcattgatg taaagccggt cctgcaaagc cctgtcccgt acgtcgtatt catgatatcc
     3601 tccgctggct atgtacagat cctggtcacc gtcattgtcg gcatcaaaaa agacagcatc
     3661 accgtcggtg cagttgagat cctcgggaaa attaaaaaac cgtgaaggcc tgaaatagcc
     3721 gtcatcaccc tggataaaca gtttccccgg gctttcgatt gtaccaccca cataaatatc
     3781 ggtcaatccg tcgccgttta cgtcggcagt ggccattacc gggccaacgg gcgaaagcat
     3841 cgaggtaagc agcggctgcc tgctgaaatc atttgaaccg tattcggtat gttcatacgg
     3901 aacaattgat tcaacgggga taaacagcct ttccggcggc ggttcaggta tgaaccttct
     3961 gttctggtcg tgccattcaa tggttataat ctggtttacc ccgacttctg ttaacagatt
     4021 aacattgcct cccggccatt ccaccctgat ggaatctatt atttcagcat cagccagtcc
     4081 gaaatgaagc tgggttgtca cacatgactg gtagcctctt gagggcatct gttcctgata
     4141 ctgaacacca tattttgtgt aaaggtaaat cttgctgcac aggcctgccg tattctttcc
     4201 gggtccttta agcctgatgc ttatatagtt ggctttggga ttatgttcac ggatcatgtt
     4261 ccggaatatg gaggctgttt cattctggtt actgacaatc aggtccagat caccgtcatt
     4321 atcaaggtcc gaataggctg ctccgtttga aaaacctttc tttgcaaatc cccagacctt
     4381 gcttttatcg gtgaatgtaa ggtctttgtt attcttgaaa atatagttgt gaacaggggt
     4441 ggaggtcatg aaactggtga gccagaaagt gtctgcaggc tccttggtct tggatttttc
     4501 aagatagtag tcatttttca tcttgaggaa atcacggtcg gtataatccc tgaaatagcc
     4561 gttggttaca aaaagatcct tccagccgtc gttgtcaaaa tcggcaaagg gcggggccca
     4621 gctccagtcg gtgttggata cgcatgcaag ctgtccgatc tcactgaaag tgccgttggc
     4681 attgttgagc tgaagcatat tgcgcattgt ggaatgataa aatccctgca ttacctgcag
     4741 ggcatattcc agataatttt cctgtccgta cagaagcttg agtctcctgt tgtcttccga
     4801 cagcatgtcg gcggtgatta tatcgggcca ggtatcgttg ttgaaatcat ttatttcatt
     4861 gcccatggcc gattgcgagt tgtgcaggac atgctcggtc atcctgttgg tgaaggtccc
     4921 gtcgccgttg ttgatataga ggtagtcagg ctcgatgtaa tcgtttgtga tgtaaatatc
     4981 aggccagcca tccttgttta tgtctgcaat ggcaattccc agaccgtatc cgatcccggt
     5041 tgaaatgatc cctgcctgac ttgtcacttc ggtaaaatga tcaccgtcat tgcggaaaag
     5101 cttgtctccg gcataggggt cgtcatcaac cctggcactc tcaaactcca ttcccctcag
     5161 taccttcgtg ttggtagcga caaggaacat gtcgagatcg ccgtcaaggt cataatcaaa
     5221 aaatgcacca agggttgagt aagtcggtac atcaataccc catttggcag cttcttcagt
     5281 aaatttcaga tcaccatggt taatgtaaag ttcattcctc ctcccttcaa ccgtttcctt
     5341 tgtgcagtag cagacgtaaa tatcaagaag gttatcgcca ttgatgtcga ccatggttac
     5401 acccgttttc cagcctggtc tgccggctac acccgcaata tcggttatgt ccctgaattt
     5461 gaaattgccc aggttgaggt aaagcttgtt gtaacccatg tttgaaatga aaaagatatc
     5521 gtcaagcccg tcattgttaa tatccccgac ggccacaccc cctccgttat agagattttc
     5581 atgggtaagg gcgttgtggt gcacattttc ggtaatgaca ttcttgaaag ttattcctga
     5641 cttgctggca ggcaccggtt taaacaactt gatgtcctta cccccggagg tggcaataac
     5701 caggatgatg agccataacc attttaacaa agcaacttta tatttgccgg aatagaatga
     5761 taaggccggt cgtttcatgg aaatattgtc ttagtacaac aaaatccgga aaagaacgtg
     5821 caatatacta ttttttttgc gcagttaatt tttgttaatt gaaaaggcga aaagcaaaat
     5881 tgccacaggt ggctacaatg atatcagata aaattgttta tttttactga tagctgtttg
     5941 atattacaac agtaatcaaa atttgattac tgtgaacact tagtaacaga ctgttgtcag
     6001 acaggtttta ccggtggttt accgaatgcc gccagtaaat ttcatgaccg gaatattcag
     6061 gcacattagt aacaaaatcc ttaatacaat gaaacgaaag atccagttca ttccttttat
     6121 tttgctgctg ttgcttttgc cgg
//
LOCUS       DAOOYG010000152         6034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_15094_length_6034_cov_4.667503, whole genome shotgun sequence.
ACCESSION   DAOOYG010000152 DAOOYG010000000
VERSION     DAOOYG010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15094_length_6034_cov_4.667503"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PK288_10390"
     CDS             <1..375
                     /locus_tag="PK288_10390"
                     /inference="COORDINATES: protein motif:HMM:NF027514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HPM19377.1"
                     /translation="GHGIGYYLNVHEGPQGISPLASSAAIESGMLLSNEPAIYREGEY
                     GIRIENIVLCYEDEISEYGCFLRFDTLSLCYIDKSLIELSLLDQREISWLNKYHSEVF
                     HRLSPYLTENERIWLREKTEGI"
     gene            391..3126
                     /locus_tag="PK288_10395"
     CDS             391..3126
                     /locus_tag="PK288_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HPM19378.1"
                     /translation="MARNPEFKKIMAAGLVLAFILSSCTGPDKRFSAVNLRCEYLQNP
                     IGIDSQKPRLTWQMADGRMGSTQSTFQVFVGTDSSKVAAGKADKWDSGEIRSDLNLVH
                     YSGGDLKPFTKYFWSVRIRDGRGRVSGISETASFETGMMKESNWKGSWIGDSHDTGLK
                     PAPYFRKSFTVSGKVNRARVYIAVAGLYELYINGHPVGDHRLDPAFTRFDRCILYNVY
                     DVSRFIQDGENVIGVLLGNGWYNHQSSAVWSFDAAPWRGRPKFCLDLNIESADGTCTS
                     IVTGTGWKTSLSPVVFNSIYTGEHYDARLEQPGWNCPGFIDTAWNDIILVAAPSRKIV
                     SQVMHPIRITETIPAKSVTRQDIHTWMFDLGRNIAGVSSLTVSGAAGTVIRLRHAERL
                     TQNGLADQSNIDVHYRPKDKSDPFQTDIFILSGKGKETFMPRFNYKGFRYVEVSSDKP
                     LNLDIESLKGHFMHSDVPIAGTIESSESLLGKIWQATNNSYLSNLYGYPTDCPQREKN
                     GWTGDAHIAIETGLYNFDGITVYEKWLNDHLDEQQPNGVLPAIIPTGGWGYHWANGPD
                     WTSTIAIIPWYIYLFYGDDQLLYNCYESIRNYVDHIDRISPSGLTDWGLGDWIPVKSV
                     ASKELTSSVYYYTDALILSKAAQLFGKAEDHRKYLSLANRIRDAINARYLDRETGCYR
                     QGFQTELSLPLFWGIVPEEIRTRVADTLAGTVKAANNTLDVGILGSKAILNALSENGY
                     PDLAYSMAVKDTYPSWGWWIRNGATTLYENWDIDPRSDISMNHIMFGEISAWMYKTPG
                     GIYPDEKQPGFRNVILKPCFVNGLDHFEARHNGPYGMIISSWRREGPEIVYDVTVPPG
                     STANLYLKGTGLSERGRKPERNKYLKILKHEGNQYHLVLSSGTFYFRIGVAGMGD"
     gene            complement(3130..5124)
                     /locus_tag="PK288_10400"
     CDS             complement(3130..5124)
                     /locus_tag="PK288_10400"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPM19379.1"
                     /translation="MKNASYESNPWPLVQNAYIRLPLGAIKPEGWLMDQLRAQAEGLT
                     GNIDEFWPDLMNSAWKGGDGEAWERGPYYLDGLVPLAYLTDDEKLKEKVGRWIEPVIS
                     SSTDSGWYGPAKNKDRWPLAVANKVLMQYYEASGDERAIEVLKGYFRYLLSAPPDWPD
                     KEWRGSRAMENAVAGYWLYRKTKEPWILKVIESVQNNSSDWTSYYEKFPWDSAASADK
                     RIPLNWGPDGLTAHVVNNAMAIKYPGLWYQQSNDSRFRDAVHEGINKYDKNHGQAGGR
                     FSGDEHLSGRSPDRGTELCAVVEYMYSLEELYAVLGDNSLADRLEMLAFNALPGTLTA
                     DMWAHQYDQQSNQVLVSADERAWSTNGKYSNIYGLMPNFACCLANMHQGWPKFTQSLW
                     MATNDNGLIAAVYGPSRVKAMVAGEIEVTVREETTYPFSGTIRFIINSSKPARFPLYL
                     RIPGWADGVTITYKDRNIMPKAGSTVRLFSRWDPDEVITMEIPMHIRFERRYNNAFSV
                     LRGPLYFSLRMEKEYKSIRMNYDNFGYKGSVDWEIRPLHPWNYGLMIDRGNPARGFEL
                     TETQPGEYPFADKGDMIWSADQGKYVKYEKDAPVVINTRGMLIPGWGMKNNSADIPPA
                     GPLMPSGTAEVIQLVPYGCAKLRITEFPVVDVTRIEDAFR"
     gene            5337..>6034
                     /locus_tag="PK288_10405"
     CDS             5337..>6034
                     /locus_tag="PK288_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HPM19380.1"
                     /translation="MKKPDNLILVIFGATGDLTSRKLIPAIYSLQKQDLLPERFELIG
                     LGRTEITSDIFRQMMEEAIVNYSGEEVDSKENVQKFLANISYRKIAYDNQEDYRNLGT
                     FISQRCSTLDIGNNLIFYMATPPSLYELIAGNLASAGLTKQDSGFRRLIIEKPFGYDL
                     ESGIKLNNTLHRLITEDQIYRIDHYLGKETVQNLLVTRFANGIFEPLWNRNYIHRVEI
                     TSAESIGVEDRGSY"
BASE COUNT         1570 a         1442 c         1481 g         1541 t
ORIGIN      
        1 gggcatggta tcggatatta ccttaatgtt catgaaggac ctcagggtat aagtccgctt
       61 gccagttcgg ccgcgataga aagcggcatg cttctttcaa atgaacctgc catttacagg
      121 gagggtgaat acggtataag gatagagaat atagtgctgt gctatgagga tgaaatatct
      181 gagtatggat gtttcttgcg gtttgacact ctttccctct gctacattga taaatcactc
      241 atcgaattat cccttctcga ccagagggaa atcagctggc tgaacaaata tcattctgag
      301 gttttccaca ggctgagtcc gtatctgaca gaaaatgaaa ggatatggct cagggaaaag
      361 actgaaggga tttgaagaaa atcaattgtt atggcaagaa atcctgaatt taaaaaaatt
      421 atggctgccg gactggtact ggcttttatc ctgtcatcat gtacaggccc ggacaaaagg
      481 ttcagcgccg tcaacctgag gtgtgaatac ctgcaaaacc cgataggcat tgattcgcaa
      541 aagccacgct taacctggca gatggccgac ggacggatgg ggtcaacaca atctacattt
      601 caggtatttg tcggcactga ctcctcaaaa gttgccgccg ggaaagctga caaatgggac
      661 tcaggcgaga tcaggtcgga tttaaatctg gtgcattatt caggtggcga tctgaagcca
      721 tttacaaaat atttctggag cgtaaggatc cgggatggca ggggaagggt gtccggtatt
      781 tctgagacag ccagctttga aacgggaatg atgaaagaaa gcaactggaa aggcagctgg
      841 attggtgaca gccatgacac cggcctgaaa ccggctcctt atttcaggaa atccttcacc
      901 gtgagtggaa aggttaaccg ggcaagagtc tacatagccg tggcaggttt gtatgaacta
      961 tatataaacg ggcacccggt cggggatcac aggctcgacc ccgcgttcac ccgtttcgac
     1021 cggtgcattt tatataatgt atatgatgtg agccgcttca ttcaggatgg agaaaatgtt
     1081 attggcgtac tcctgggaaa cggctggtac aatcatcaat catcggcagt atggtctttt
     1141 gatgctgctc cgtggcgcgg aagaccgaaa ttctgcctcg atctgaacat cgagtccgcg
     1201 gacgggactt gtacaagtat tgtcacagga acaggatgga agacatccct gagtcctgtc
     1261 gttttcaaca gtatttatac cggcgagcat tatgatgcca ggctggaaca gccggggtgg
     1321 aactgtcccg gattcattga tacagcctgg aacgatatca tacttgtggc agctccttcc
     1381 cggaagatcg tttcccaggt catgcacccc ataaggatta ctgagacgat accggcaaag
     1441 tcggttaccc gccaggacat ccatacctgg atgtttgatc tcggaaggaa tattgccggt
     1501 gtgagcagcc tgaccgtaag cggtgccgca ggaacggtaa taaggctcag gcatgctgaa
     1561 aggctcactc aaaacggact ggccgatcag tcaaacatcg atgtccacta caggccgaaa
     1621 gacaaaagcg atccgtttca gaccgatata ttcattcttt ccggcaaggg aaaggaaacc
     1681 ttcatgcccc ggtttaatta caaggggttc agatatgtgg aggttagctc cgataaaccg
     1741 ctgaaccttg acattgaaag cctgaaaggg cacttcatgc attccgatgt tcccatagcc
     1801 ggaaccattg aatcatctga atccctcctg gggaaaatat ggcaggcaac gaacaattca
     1861 tacctttcaa atctttacgg ctatccgacc gactgtccgc agcgtgagaa gaatggatgg
     1921 accggcgatg cccatattgc gatagaaaca gggctgtata actttgatgg tataacggtg
     1981 tatgaaaaat ggctcaatga tcacctcgat gaacagcagc caaacggggt tcttcctgca
     2041 ataatcccta ctggcggttg gggttatcac tgggcaaacg gacccgactg gaccagtacc
     2101 attgcgatca tcccatggta tatttatctt ttttatggtg atgatcagct gctgtacaat
     2161 tgctacgaaa gtatccggaa ctatgtggac catattgaca ggatcagtcc ttcggggctt
     2221 accgactggg ggctgggtga ctggatcccg gtgaagtcgg tcgcctcaaa ggagctcact
     2281 tcttccgttt attattatac cgacgcactt atactgtcga aggcagcaca attattcggc
     2341 aaggctgagg atcaccggaa atacctttcc ctggcgaaca ggataaggga tgcaatcaat
     2401 gccaggtatc tcgatcgtga aacaggctgt taccgtcagg gtttccagac agagctgagt
     2461 cttccccttt tctgggggat agtcccggag gaaatcagga ccagggttgc agacaccctg
     2521 gccgggacgg taaaagccgc caacaatacc ctggatgtcg gcatccttgg ttcaaaggct
     2581 attcttaacg cattgagcga gaacggatat cccgacctgg catacagtat ggcagtaaaa
     2641 gatacctatc cctcgtgggg atggtggatc aggaacgggg ccaccaccct ctatgagaac
     2701 tgggatattg atccccggtc ggatatatca atgaatcaca taatgtttgg tgaaatcagc
     2761 gcctggatgt ataaaacacc cgggggaata tatcccgatg aaaaacagcc cggattccgg
     2821 aatgtgatac tcaaaccctg ctttgtaaac gggctggatc attttgaagc caggcataac
     2881 gggccatatg gtatgataat atcttcatgg agaagagagg gcccggaaat tgtatatgat
     2941 gttacagttc cacccggaag caccgcaaat ctttacctta agggaaccgg cctgtcagag
     3001 aggggtagaa agcctgaaag gaacaaatac ctgaaaatac tgaaacatga aggtaatcag
     3061 tatcatcttg tcctttcatc aggaaccttt tatttcagga tcggagttgc cggaatggga
     3121 gattgattct taccggaatg catcttcaat cctggtaaca tcgacaacgg gaaattcggt
     3181 tattctgagt tttgcacagc catacggaac cagttgaatt acttctgctg ttcctgaagg
     3241 cataagggga cctgcagggg ggatgtctgc cgaattattt ttcattcccc atccggggat
     3301 cagcattcct cttgtgttga tcactaccgg agcatctttt tcatatttca cgtatttccc
     3361 ctgatctgcc gaccagatca tatctccctt gtctgcaaac ggatattcgc ctggctgggt
     3421 ttcagtcaat tcaaatcctc tggcgggatt accccggtca atcatcagtc cgtagttcca
     3481 ggggtgaagt ggcctgattt cccagtcgac gcttcccttg tatccgaaat tatcataatt
     3541 cattcttatg ctcttgtatt ccttttccat cctgagtgaa aaataaagcg gacccctcag
     3601 cacagaaaat gcattattgt atcttctttc aaacctgatg tgcattggaa tctccattgt
     3661 gatcacttcg tcagggtccc acctggagaa cagccggacg gttgaaccgg cttttggcat
     3721 gatgttccgg tccttgtacg tgattgtcac accgtcggcc cagcccggga ttctcaggta
     3781 aagagggaac cttgccggtt tgctgctgtt tatgatgaat cgtattgttc cgctgaaagg
     3841 gtatgtagtc tcttccctga cggtcacctc aatttctccg gcaaccatgg cttttaccct
     3901 cgacggacca tataccgccg cgatcagtcc gttgtcgttc gtggccatcc agagtgactg
     3961 ggtgaatttt ggccagccct ggtgcatgtt ggccaggcag catgcgaagt tgggcatcag
     4021 gccatatatg tttgaatatt tcccgtttgt gctccaggct ctttcatccg cgcttacgag
     4081 gacctgattt gactgctggt catactggtg ggcccacatg tcggcggtta atgttccggg
     4141 gagtgcattg aaagccagca tttccagcct gtcggcaagt gagttgtcac ccaggacggc
     4201 atacagttcc tccagagagt acatatattc gaccactgca cagagttcgg tgccccggtc
     4261 agggcttctg cccgaaagat gctcatctcc cgagaaacga cctccggcct gtccatggtt
     4321 tttatcgtac ttgtttatgc cttcatgcac ggcatcacgg aacctgctgt catttgattg
     4381 ctggtaccag aggccgggat acttgattgc catggcattg ttgacaacat gtgccgtcag
     4441 gccgtcaggg ccccagttca gggggatcct cttatctgcc gatgccgccg aatcccatgg
     4501 gaacttctca taataggatg tccagtcgga gctgttattc tggaccgact caataacctt
     4561 cagtatccat ggttcctttg ttttccggta cagccagtaa cctgccacgg cattttccat
     4621 ggcccttgaa cctcgccatt ccttatcggg ccagtcgggc ggagctgaaa gcaaatacct
     4681 gaaatatcct tttaaaactt caattgccct ctcatcgccg gaggcttcgt agtattgcat
     4741 caataccttg tttgccacag ccagcggcca acggtccttg ttttttgcag gcccgtacca
     4801 tccgctgtca gtgctgctgg aaataacagg ttcaatccac ctgccgacct tctcttttaa
     4861 tttctcatca tcagtcaggt atgccagcgg aacaagcccg tcgaggtaat atggacccct
     4921 ttcccaggct tcgccatcgc ctcccttcca tgccgaattc ataagatcag gccagaattc
     4981 atcaatattt ccggtaagcc cttctgcctg tgccctgagc tggtccatca gccagccttc
     5041 gggctttatt gcaccaaggg gcagcctgat gtaggcattc tgcaccaggg gccatggatt
     5101 ggattcataa cttgcatttt tcatttcatc attacatccc gtcaataaaa ggcaaaccag
     5161 aaaaaaacgg actgctgttt tcatggtact cgtcggatca tgtgttatat ttctgtatta
     5221 tcagtaccaa tatcgcagaa aaattcgata tgtttataaa ataccggcaa cattatgacg
     5281 ccgatcagtg ttataggttt taatttgccg gataagagat taaaaacact tagagcatga
     5341 aaaaacctga caacctcatc ctggttatat tcggagcaac gggagatctc acctcgcgta
     5401 agctgatacc agccatctat tcattgcaga aacaggatct acttcccgag aggttcgaat
     5461 taatcggttt aggcagaaca gagataactt cagacatttt caggcagatg atggaagaag
     5521 ccattgtcaa ctactcaggc gaagaggttg attcaaagga gaatgtccaa aagttcctgg
     5581 caaatatctc gtacagaaaa attgcctatg acaatcagga agattacagg aatctcggga
     5641 cgttcatttc gcaaaggtgc agtaccctgg atataggaaa caacctgatt ttttacatgg
     5701 caaccccacc cagcctttat gaattgattg ccggcaatct ggcttcagca ggcttaacga
     5761 agcaggacag cggtttccgg cggctgatca ttgaaaaacc tttcggatac gaccttgaat
     5821 ctggaataaa gcttaacaac accctgcaca ggctgatcac ggaggaccag atctaccgga
     5881 tagatcatta cctggggaag gaaaccgtac agaacctcct ggtaacccgg tttgccaacg
     5941 ggatattcga acctttatgg aacaggaatt acatacatcg ggttgaaata acctcagccg
     6001 aaagcattgg ggttgaggat cgcggaagct atta
//
LOCUS       DAOOYG010000153         5948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_15411_length_5948_cov_9.081453, whole genome shotgun sequence.
ACCESSION   DAOOYG010000153 DAOOYG010000000
VERSION     DAOOYG010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15411_length_5948_cov_9.081453"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..456
                     /locus_tag="PK288_10410"
     CDS             67..456
                     /locus_tag="PK288_10410"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPM19381.1"
                     /translation="MEINHPKSTYINRPAGEKYHMGIIWDFDWGFGFEVKFKHYDLST
                     AALPLFWEQNTAGKTFFSRLMEDPVVKDLFAERWAWFKTNAYEELKDYVITWSVLIQN
                     ALPKDHEIWGKRGSSGDASTDLERVLE"
     gene            654..1109
                     /locus_tag="PK288_10415"
     CDS             654..1109
                     /locus_tag="PK288_10415"
                     /inference="COORDINATES: protein motif:HMM:NF039475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6508 domain-containing protein"
                     /protein_id="HPM19382.1"
                     /translation="MKVLKDDNYREKINSYTKQDWKPLFDLIPLIEQTDKFGDDTEAM
                     KSLEKGIFIMDPYEEHHVVEQFREAVYAIPVMIDFDWSAWDEGRKIVSDDNFDYDSID
                     IPTKCKIISAIVRNDRFCSGRLVSAFETGMMLKVLRSIQRQAEPGEPAK"
     gene            1501..2097
                     /locus_tag="PK288_10420"
     CDS             1501..2097
                     /locus_tag="PK288_10420"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HPM19383.1"
                     /translation="MSIIPDTDVFRSIQKGDHKAFSQLFDSYYISLCFFAARYLHDLS
                     LARSLVQDLFIDLWQRREKIEVAVSVKAYLYISVKNRCIDYLRAKRPEAGITEPEAAV
                     SQVPFRDLIEEAELNERINLSINRLPDKCREIFLLSRMEGLKYAEIARKLDLSVKTVE
                     MQMGIALRKIRESLSDYLTLLLLAFLCKKLFMLLQGVL"
     gene            2130..3143
                     /locus_tag="PK288_10425"
     CDS             2130..3143
                     /locus_tag="PK288_10425"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPM19384.1"
                     /translation="MEETNKLDILIGKIISGNASPEELQQAEDLRNQDPGITDFLAQS
                     RKAWDNTRYFLSPEDYGSDKQLLGSKYSEFLFGKIWLVSRRVLIYRIAAILAFPVALA
                     AGWYGFGKGFLRSSLASQVCVISSPPGNVSKTILPDGTWIWINTGSSVSYNTAAFNHG
                     KREVQLTGEAYFQVSPDKGRPFIVETPHASVKVTGTSFNIKAYPDDPDFETVLAEGSV
                     ELSLKQADSRVIRMNPGQRIMYNSRNDSVNINEVDAEIYTSWRNMELIFKDASLNDLV
                     RKLDSIYDIRFHLEPASLGDYRFRGMFRYNNNLIEALEKIKKTSGINYYIENKEVWLR
                     IAK"
     gene            3286..>5948
                     /locus_tag="PK288_10430"
     CDS             3286..>5948
                     /locus_tag="PK288_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM19385.1"
                     /translation="MNYFRKPLRELPFQQKKLLLIVQLSLLLFLFPVLSSHASSAYAE
                     KTRLTIKLDNVRYSDVFDAIEKQSEFYFFYNRDYFNDRRLISVDFKDVLINDLLNELF
                     RNDGITYEISDRSILLRVPESRSAEGSRVASTQQRTISGTVANSSGEFLPGVTVVEKG
                     TGRGTITGNDGKFSLAVSDNSAILVFSFVGMKTQEVPLEGRVNISVVMIDETVGLDEV
                     VVIGYGTQKKINLSGAVDVVTSKDIENRPVNNAVDALQGLAPNLNLSIGNEGGELGAK
                     HKLNIRGIGSISGTGGNPYILIDGVEGDINLINPSDIESISVLKDASASAIYGARAAF
                     GVILVTTRKGSSSGSLINYSNNLSFSDPTVLPKIVNSLRWAEYFNLASANDGDSPIFS
                     NETIEKIKKYQRGELKDWTEPYEVEPMFWKSYQDSWANTDWYKEEYRRWAPKSTHNIS
                     ISGGDKNTQYYISGATFNQKGLIKWGDDKYTRNNVNAKVNTQINSWLRANFQTRYDKS
                     ELTGPSYNTDLWYHNISRRWPTNGVYTPDGNLFYEMEQVWLKYGGRARTESNQLNIIP
                     GLEIEPVSGLVLHANYRYRKNSETSEAHNSKIKVTLANGYEAYLEPNNSIEERSGEEI
                     YNSPNVYLDFNKKIGGHEIMILAGYEQEVIRYSSLYGRKDDIITDNVPSMSTATGKEY
                     ASGTKGHWATQSYFSRLNYSFQGKYLLELSMRRDGTSKFASGHRFGNFPSGSVAYIIS
                     QERFFEPLSQYINFLKFRASYGSLGNQDVANYLYVETLPISSRLAYILDFDRPNFAGM
                     PGISSPTLTWEKVRTANFGADISFLKNRLTGSFDYFVRKTLDMLGPAESLPALLGTAV
                     PQSNNATLRTKGFELSVRWRDQIHQFQYSVA"
BASE COUNT         1732 a         1312 c         1416 g         1488 t
ORIGIN      
        1 gaactattga aaaacgtcta attttgattg gcccgaaata tagggaagct tacacttact
       61 ctgaatatgg aaattaatca cccaaaaagc acctatataa ataggccggc cggagaaaaa
      121 taccatatgg gaattatatg ggattttgat tggggttttg gctttgaggt taaatttaag
      181 cattatgact tatcaacagc agcattgcct ctattctggg aacaaaacac agcaggtaaa
      241 acatttttta gcagattaat ggaagaccca gttgtgaaag atttatttgc tgaacgatgg
      301 gcctggttca aaaccaatgc ttatgaagaa ttaaaagact atgtaattac ctggtctgtt
      361 ttaattcaga acgctttgcc aaaagatcat gaaatctggg gtaagcgtgg atctagtgga
      421 gatgcttcta cagatcttga gagggtactt gaatgacttg atgccagagt tgcctacatt
      481 gatacctatg tggcaggatt ttaatccagt aatgtcttaa cctcccaaat agctggacat
      541 aacttgaaaa acaaattagg tctgattaat ttggggaatc atgggaaagg aaacaggttc
      601 aatggcctga ttacggcgag acagatataa ggaacaaaca attcaatacg gtaatgaaag
      661 tattaaaaga tgataattac cgggaaaaaa tcaattctta cacaaagcag gactggaagc
      721 cgctatttga tctgatcccc ctgattgaac agacagacaa attcggtgat gatacggaag
      781 ccatgaagtc gctggaaaag ggtattttca ttatggatcc ttatgaggaa caccatgtag
      841 ttgaacagtt ccgcgaagct gtttatgcaa taccggtcat gattgatttt gactggtctg
      901 cctgggatga aggcaggaaa atagtcagtg atgataattt cgactatgat agcatcgata
      961 taccaaccaa atgcaaaata atatcggcaa ttgtccggaa tgatcgtttt tgcagcggca
     1021 ggctggtaag tgcttttgag accggaatga tgcttaaagt gctcagatct attcaaaggc
     1081 aggcggaacc aggggaaccg gcaaaatgac cgcaagttac tcataactgt aaggtacagc
     1141 tgaaataacc ggcttatgga cattgcggaa ggagtatttc agaagcctcg cacccccgcc
     1201 cgtacacggg aataaggggt tattaagcag atcttccggc tgccgggatc aaaacaccgg
     1261 aacatattga gatacacggg aagccggagc cttcatcctt gcggtgttta tgtgcaccgg
     1321 gaacctgcat gaaaatactg ccggcatttc actactggcg gatctgcatg ctgatcagcc
     1381 tgttcccccc gcctccttca ctgaaacttt cgcatattgg tcccggttcc tgaccgcggg
     1441 ccgggctgtg atattttccg tgcttttttt ctacttttag caggctattg gggttcagct
     1501 atgagtatca taccggatac ggatgttttc aggagcattc agaaaggaga ccataaggct
     1561 ttcagtcaac tttttgacag ctactacatt tcattgtgtt tctttgctgc caggtacctt
     1621 catgatctca gcctggcccg ctcactggtc caggatcttt tcatcgatct gtggcaaagg
     1681 agggaaaaaa ttgaagtggc cgtttcggtg aaggcatatt tgtacatctc cgtaaagaac
     1741 agatgcattg attatctcag ggcaaaaaga cccgaagcgg gaatcacgga gccggaagca
     1801 gccgtcagcc aggtgccctt ccgcgatctt atagaagagg cggaactgaa tgaacggata
     1861 aatctttcaa taaaccggct tcccgacaag tgccgtgaaa tattcctgct gagccgcatg
     1921 gaaggcttaa agtatgccga aattgcaaga aaactggatc tttctgtaaa aacggtggag
     1981 atgcagatgg gtatagcctt aaggaaaata agggagagtt tgtccgatta tttaactctc
     2041 ctgctccttg catttctttg taaaaaatta ttcatgctgt tacagggtgt cctttgattt
     2101 tttcgtctta tccaaaaaag atttcagaat tggaagagac gaataaactc gacatcctta
     2161 tcgggaagat catatcgggc aatgcttccc ctgaagagct gcagcaggcg gaggacctcc
     2221 ggaaccagga tcccggtatt acagatttcc tggcacaaag ccgcaaagcc tgggacaata
     2281 cgcggtattt cctttcgcct gaggattatg gctcagataa gcagctgctc gggtcgaaat
     2341 acagtgaatt ccttttcggg aaaatatggc tggtgagccg aagggtactg atatacagga
     2401 ttgcggccat tttagctttc cctgttgcac tggcagccgg atggtatggt ttcggaaaag
     2461 gattcctcag aagcagcctg gcttcacagg tctgtgttat ttcatcgccg ccgggcaacg
     2521 tctcgaaaac aattctgccc gacggaacct ggatatggat caataccggt tcatcggttt
     2581 catacaacac cgctgcattc aaccacggga aaagggaagt tcagctgact ggcgaggcct
     2641 atttccaggt ttcccccgac aagggcaggc ctttcatcgt tgaaacccct catgccagtg
     2701 tgaaagtgac tggtacatcc tttaacatta aagcctatcc tgacgatcct gattttgaaa
     2761 cggttcttgc cgaaggcagt gtagagctca gcctgaagca ggctgacagc agggttatcc
     2821 gcatgaatcc cggtcagcgg atcatgtaca attcacgaaa cgacagcgtg aacataaacg
     2881 aggtagatgc tgaaatatat acctcgtggc gcaacatgga actgatcttc aaggatgcct
     2941 cacttaacga cctggtacgt aagcttgaca gtatctacga tatcaggttt catcttgaac
     3001 cagccagcct cggcgattac cggttcaggg gaatgttcag gtacaataat aacctgatcg
     3061 aggcgctgga aaaaatcaaa aaaacatccg gcattaatta ttacattgaa aataaggaag
     3121 tctggttaag aattgcaaaa tgaactgcct atgacaaaga aaagaagagg atttctgatc
     3181 acgtactgaa atattgaaaa agagggaaac tgtggtggtt tcccctcttt tggaatttca
     3241 ttagttcttg aaaaactaac tgttaattaa aaaacactca aatctatgaa ttattttaga
     3301 aaaccgctgc gggaattgcc ctttcagcaa aaaaagttgt tgctgattgt gcaattgagt
     3361 ctccttctct ttctatttcc cgttttatct tcccatgcct ccagtgccta cgctgagaaa
     3421 acaaggctca cgataaaact ggacaatgtc agatactcgg atgtattcga tgccattgaa
     3481 aagcaatcgg agttctattt tttctataac cgcgattatt tcaatgacag gcgtcttatt
     3541 tcagtcgact ttaaggatgt actgataaat gatcttttaa atgaattatt caggaatgat
     3601 ggaatcacct acgaaatttc cgatcggagc attttactga gagttcccga atccaggtca
     3661 gctgaaggaa gcagggttgc ttcaacacag cagcggacca tctcaggcac ggttgccaat
     3721 tccagcggag aatttctgcc cggcgttacc gttgttgaaa aaggtacggg ccgggggacg
     3781 ataaccggca atgacgggaa attctcactt gccgtttctg ataattcagc aatcctggtt
     3841 ttttcctttg tagggatgaa gacacaggaa gttccgcttg aaggaagagt aaatatttcc
     3901 gtcgtgatga tcgatgaaac ggtcggactc gacgaagttg tggtgatcgg ctatggcacc
     3961 cagaaaaaga taaatctttc cggggccgtt gatgttgtga catcaaagga tatcgaaaac
     4021 cggccggtta acaatgccgt tgacgctttg cagggcctgg cgccgaacct caacctgtcc
     4081 attggcaatg aaggaggcga gctgggggca aagcacaagc tcaatatcag gggcataggc
     4141 tccataagcg gaacgggagg gaatccctac atcctgatag acggggttga aggtgatatc
     4201 aacctgatca acccgtcgga tattgaaagc atctctgttc tgaaggatgc ttctgcatca
     4261 gccatatacg gagcccgagc agcatttggc gtcatcctgg taaccaccag gaaaggatca
     4321 tcctctggct ccctgataaa ttactcgaac aatctgtcct tctcagatcc gacggtactt
     4381 ccaaaaatcg tgaattcact cagatgggcc gaatacttca accttgcatc tgccaatgac
     4441 ggtgattcac ccatattctc gaatgaaaca attgaaaaga tcaagaaata ccagagaggt
     4501 gagctcaagg actggacaga gccctatgag gtggagccca tgttctggaa atcataccag
     4561 gattcctggg ccaataccga ctggtacaag gaggaataca ggcggtgggc tccgaaatcg
     4621 actcacaata tcagtattag cggcggagac aaaaacaccc agtattatat ttcaggcgcc
     4681 accttcaacc agaaaggcct gataaaatgg ggcgatgaca aatatacccg caataacgta
     4741 aacgccaagg tcaatacaca gattaacagc tggctgcgtg ccaattttca gaccaggtat
     4801 gacaaatccg aactaaccgg acccagctac aataccgacc tgtggtatca caatataagc
     4861 cggcgctggc ccaccaacgg cgtatacaca cctgacggga acctgtttta tgaaatggaa
     4921 caggtatggc ttaaatacgg cgggagagcc cggaccgaga gcaaccagct caacataatt
     4981 cccggcctgg agattgagcc ggtcagcgga ctggtgcttc atgccaacta ccggtacagg
     5041 aagaattcag aaacttcgga agctcacaat tccaaaatca aggtcaccct cgccaacggt
     5101 tacgaagcct acctcgaacc caataattcc attgaagagc gaagcgggga ggagatctat
     5161 aattcaccga acgtttatct cgacttcaat aaaaaaatag gcggacatga aataatgatc
     5221 ctggccgggt atgagcagga agtcatcagg tattcctcac tttatggccg gaaagacgat
     5281 attatcaccg acaatgttcc ttcgatgagt acggccacgg gcaaggaata tgcttcggga
     5341 accaaggggc actgggccac acagagttat ttttcaaggc tgaactacag tttccaggga
     5401 aaatacctgc ttgaactctc aatgcggcgc gatggcactt ccaaatttgc aagcggtcac
     5461 cgcttcggga attttccatc gggctcagta gcatacatta tttcgcagga gcgttttttt
     5521 gaacctctct cacaatacat aaattttctc aaattcaggg cttcatacgg ttcgctggga
     5581 aaccaggatg tagcgaacta cctgtatgtt gaaaccctgc ctatttccag cagactggct
     5641 tacatactcg atttcgacag gccaaatttc gcaggaatgc ccgggatctc gagtccgacc
     5701 ctcacctggg aaaaggtccg tacggcaaat ttcggtgcag atataagctt tctcaagaac
     5761 aggctgacgg gatcatttga ttatttcgta agaaagaccc ttgacatgct cggcccggct
     5821 gaatcccttc ccgcattgct gggaacagct gtgccccaga gcaataatgc taccttgcga
     5881 acaaagggtt ttgaactgtc agtgaggtgg agagatcaga tccaccagtt tcaatattcc
     5941 gtcgcatt
//
LOCUS       DAOOYG010000154         5928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_15481_length_5928_cov_8.563937, whole genome shotgun sequence.
ACCESSION   DAOOYG010000154 DAOOYG010000000
VERSION     DAOOYG010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15481_length_5928_cov_8.563937"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PK288_10435"
     CDS             <1..1000
                     /locus_tag="PK288_10435"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HPM19386.1"
                     /translation="LTKGKSWSDIVHWPYKRLAGFGKVGHNDAIVFNFPAGDTVVVEE
                     QATSYYEIVRRTARELMARDAYANQASKTKDYYMRQARKEVRERNHIIYRPVDRRDNY
                     VKRCIGLPGDTLQIEGGKVFVDHKPVPENETQQAEYIVSTNGTTKNPKAFERMGVYRS
                     DQNMISGSAYLLPLTRKNAEAISKFANVTNVTPVFARKGEYAPHIFPYSRTYGWNEDN
                     FGPLWVPSKGAVVSLDTSNLCLYERIIDVYEGNDLKVEGDRIFINGEPADSYKFKMDY
                     YFMMGDNRPNSADSRYWGFVPEDHIVGKPKFIWLSIDKESTGLKKFRLKRMFMRIR"
     gene            complement(1214..1558)
                     /gene="rplT"
                     /locus_tag="PK288_10440"
     CDS             complement(1214..1558)
                     /gene="rplT"
                     /locus_tag="PK288_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPM19387.1"
                     /translation="MPRSVNAVASRARRKKVLKQTKGNFLSRKNVFTVAKNTLEKGLT
                     YAYRDRKKKKGEFRSLWIQRINAGVRQYDMSYSEFMGKLEKKGIALNRKTLADLAMNH
                     PEAFKAVVDKVK"
     gene            complement(1653..1850)
                     /gene="rpmI"
                     /locus_tag="PK288_10445"
     CDS             complement(1653..1850)
                     /gene="rpmI"
                     /locus_tag="PK288_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPM19388.1"
                     /translation="MPKMKTNSGAKKRFTLTGTGKIKRKHAFKNHILTKKSTKRKRNL
                     RYFGTVDKADMKNIKLLLTNK"
     gene            complement(1873..2409)
                     /gene="infC"
                     /locus_tag="PK288_10450"
     CDS             complement(1873..2409)
                     /gene="infC"
                     /locus_tag="PK288_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPM19389.1"
                     /translation="MRRSPARVVQQAHRINNRITSKVVRVVGEDIETAVMSIHDALKL
                     ADSLELDLVEISPNADPPVCKVVDYQKFLYQQKKKQKEIKAKTTKVVIKEIRFGPNTD
                     DHDYNFKLKHAIKFLEEGAKVRAYVFFKGRSILFKEQGEVLLLRFANDLEEYGKVEQL
                     PKLEGKRMTISLSPKKKK"
     gene            complement(2469..4409)
                     /gene="thrS"
                     /locus_tag="PK288_10455"
     CDS             complement(2469..4409)
                     /gene="thrS"
                     /locus_tag="PK288_10455"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPM19390.1"
                     /translation="MIKITFPDGSVKEFEPGISSLEIAEQISPRLAGEVYAATVNNEV
                     RDLTRPIYADASLVLHKWDDEPARHAFWHSSAHLMAEALESIYPGIKFGIGPAIENGF
                     YYDVDPGDGRVITDADLPAIEKKMKELAAENLKFTRREVSKKEAMDIFTAKGDEYKTE
                     LIGELEDGSITLYTQGNFTDLCRGPHLPSTAPIRAIKLLNVAGAYWRGNEKNKMLTRI
                     YGITYPKQKLLDDYLVFLEEARKRDHRRIGRELELFTFSPHVGMGLPLWMPKGAELRQ
                     NLIDFMTRILKKRGYQIVTTPHIGNVNLYKTSGHYEKYGKDSFQPMATPQEGEQFMLK
                     PMNCPHHCEIYNAKPHSYKDLPFRMAEFGTVYRYEQSGELHGLTRVRSFTQDDAHHFC
                     RHDQLKEEFKSIIDLVQFIFKAVNLNEYSAQISLRDPDNREKYIGSDENWEKAENAII
                     EAAGEKELETTVVPGEAAFYGPKLDFMVKDAIGRKWQLGTIQVDYNLPERFDLTYNGS
                     DNQKHRPVMIHRAIFGSLERFIAVLIENTAGKFPLWLAPEKAVVLPISEKFNDYARKV
                     FDFLNNSDICTLIDERSEKIGKKIRDNELKRIPYLLIIGNNEAVNETVSVRRQGEGDR
                     GTMKPEEFADLVRKEIAGEITF"
     gene            4585..5325
                     /locus_tag="PK288_10460"
     CDS             4585..5325
                     /locus_tag="PK288_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPM19391.1"
                     /translation="MNKVFYITFILPLILISCEYSPRAFFSADPGDPVVGEEVWFTSG
                     SDNAVEHEWDFGDGFYSTERNPVHKFMSSGKFTVTLKVWSSSGLSDEASLGINVLIPT
                     LLEIEVLEYYEEYPVKGASVILYPTLGDWENERNMTGEGITDSDGKVIFAGLGKKVHY
                     VDVWEKNHDNYALKEEDIGFIRTPEIIPNRINRFIAWVDYVDHGKAGRRDASVVVRKL
                     ERKPSEKKQPEASDGTSGWEELYNRRVR"
     gene            complement(5383..5769)
                     /locus_tag="PK288_10465"
     CDS             complement(5383..5769)
                     /locus_tag="PK288_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19392.1"
                     /translation="MKKLLLLLLIVAASCATTKQSVQEDSLIITRKYVGNFVDYRQHI
                     PEKAGEPYLIFIKTSMDSTYGKISAFSERCDFVKGSPLYIRRTMMSPGTISTYWEYRI
                     ESENSEIFYRLSEFQHDRKNLIQSWF"
BASE COUNT         1434 a         1356 c         1367 g         1771 t
ORIGIN      
        1 tctcacaaaa gggaagtcgt ggtcggacat agttcactgg ccttacaaaa gactggcagg
       61 atttggcaag gtaggacaca atgatgcaat agtctttaat tttcctgcag gcgacactgt
      121 tgttgtcgaa gaacaggcaa ccagttatta tgaaatagtc agaaggacgg cccgtgaact
      181 gatggcgagg gatgcctatg caaatcaggc atcgaagact aaggattatt atatgcggca
      241 ggcaaggaag gaagtcaggg aaaggaacca tataatttac cgtccggtcg acaggaggga
      301 taattatgta aaaaggtgca ttggcctccc gggcgacact ctccagattg aaggaggcaa
      361 ggtgtttgtg gatcataagc cggtgcctga gaacgagacc cagcaggctg aatatatagt
      421 aagcaccaac ggcactacaa aaaatccgaa ggcatttgaa agaatgggtg tttaccggtc
      481 tgatcagaac atgatatcag gttcggctta cctgctgccg cttacaagga agaatgccga
      541 agcgatctca aaatttgcca atgtgacaaa tgtcacaccg gtctttgcca ggaaagggga
      601 atatgccccc catattttcc cgtacagcag aacctacgga tggaacgaag ataatttcgg
      661 accgctgtgg gtgccgtcga agggcgccgt tgtcagcctc gacacatcaa atctgtgcct
      721 gtacgagagg atcatcgatg tttatgaagg caatgacctg aaggtcgagg gagaccgtat
      781 tttcataaac ggtgaaccgg ccgattccta caaattcaaa atggattatt atttcatgat
      841 gggcgataat cgtccgaatt ctgctgattc acgctactgg ggtttcgttc ccgaggatca
      901 tatcgtggga aaaccaaaat tcatctggct gtcgattgac aaggaatcca ccggcctgaa
      961 aaagttcagg cttaagcgga tgtttatgcg gatcaggtaa ttcttttcca aaagggtcag
     1021 tcaggcccgt caatggaaca tcgggcgcaa tagtttgtcg ggcagttatg catatcccgg
     1081 ccggaaatgc cgtagcattg cggaatatct tcagatgatc gccgttaata cgccgatctt
     1141 agattccggt gtaaatccgt gaataaacgg ggcgaattct gccttctgaa cggcagttat
     1201 tataattctt aggctatttc accttgtcga ccaccgcctt gaaggcttcc gggtggttca
     1261 ttgccaggtc ggcaagtgtt ttcctgttga gtgctatgcc cttcttttca agcttgccca
     1321 tgaactctga atagctcatg tcatattgtc ttacccccgc attgatcctc tgtatccaga
     1381 gcgacctgaa ttcacctttc tttttcttcc tgtcacgata ggcataggtt aatcctttct
     1441 cgagggtgtt cttggcaacc gtgaatacgt tcttccttga caggaagttg cctttcgtct
     1501 gcttgaggac ctttttgcgc ctggctctcg atgctactgc atttactgat cttggcataa
     1561 ctgatttttt ttgaatgaca gcgatgcgcc gtctgcgcac tcttgattgc tgtacaatcc
     1621 gttaatactt tttgttaaat catgcaaacg tgctatttgt tcgtgagaag aagctttatg
     1681 ttcttcatgt cggccttgtc aaccgtcccg aaatatctga ggtttctttt ccgcttggtt
     1741 gactttttgg tcagtatatg atttttaaat gcatgtttgc gttttatttt ccccgttccg
     1801 gtaagagtga atctcttttt tgcaccggag tttgtcttca tctttggcat ttcgtctaac
     1861 aataaattaa aatcatttct tctttttcgg ggacagggat atcgtcatcc tttttccctc
     1921 aagttttggc agctgttcca cctttccgta ttcctcaaga tcgtttgcga atcgcagcaa
     1981 aagcacctca ccctgttcct tgaacagaat tgatctgccc ttaaaaaaca cgtatgccct
     2041 tacttttgca ccttcttcaa ggaatttaat ggcatgtttc agcttgaaat tataatcgtg
     2101 atcgtcagta ttcggaccga atcttatttc ttttataaca accttggtgg tttttgcctt
     2161 gatttccttc tgtttctttt tctgctgata aaggaatttc tgataatcca ccaccttgca
     2221 aaccggggga tcggcattcg gggatatttc aacaagatcg agttccagtg aatctgccag
     2281 cttaagggcg tcatgaatac tcataaccgc tgtttcgata tcttcgccaa ctaccctgac
     2341 cacctttgaa gtgatcctgt tgttaattct gtgggcctgc tgtacaaccc gcgcggggct
     2401 tcgcctgatt ggtcctgcta tggctatttc ctcctgtctt ttgttaatac taattttaca
     2461 aatcaaattc aaaaggtgat ttcccccgca atctcttttc gcacaagatc agcaaattct
     2521 tcaggtttca tggttcccct gtcgccttct ccctgcctgc gcactgaaac cgtttcgttc
     2581 acggcttcat tatttcctat gataagcaga tagggaattc tctttaattc attatccctt
     2641 atcttcttac cgatcttttc actcctttcg tcaataagtg tgcaaatatc ggaattattt
     2701 agaaaatcaa aaacttttct tgcataatca ttgaattttt cactgatagg cagcacaacg
     2761 gccttttcag gtgcaagcca gagcgggaat tttcccgctg tattttcgat taggaccgcg
     2821 atgaatcttt caagtgatcc gaatattgcc ctgtggatca tcaccggcct gtgtttctga
     2881 ttgtcacttc cgttgtaggt gaggtcgaat ctttcgggca ggttgtaatc gacctggatc
     2941 gttccgagct gccatttcct gccaattgca tctttgacca tgaaatcgag tttggggccg
     3001 taaaacgcag cctcaccggg aacaacagtg gtttcaagtt ccttttcacc ggccgcttca
     3061 atgattgcgt tctcggcttt ttcccagttc tcatccgatc cgatgtattt ctccctgtta
     3121 tcaggatcac gcaaggatat ttgtgcggaa tactcattaa gattgacggc cttgaaaatg
     3181 aactgaacca ggtcgataat gctcttgaac tcttctttca gctggtcgtg cctgcagaaa
     3241 tggtgggcat catcctgggt gaagcttctg acccttgtga gcccgtgcaa ctcaccgctt
     3301 tgctcatacc tgtatactgt cccgaattca gccatccgga atggaagatc cttgtatgag
     3361 tggggtttgg cattgtatat ctcgcagtga tgggggcagt tcatcggttt aagcatgaat
     3421 tgttcgcctt cctgcggtgt tgccatgggc tggaatgaat ccttcccgta tttttcgtag
     3481 tgccctgagg tcttataaag attaacgttc ccgatatgag gggtggtaac aatctggtag
     3541 cctcttttct tcaggatcct ggtcatgaaa tcaatcaggt tctgcctcaa ctcggctcct
     3601 ttgggcatcc acagaggaag ccccatgccc acgtgcgggg aaaatgtaaa aagctcaagt
     3661 tccctgccaa tcctgcggtg atcccttttc ctggcttctt caaggaatac caggtaatca
     3721 tcgagcagtt tctgctttgg atatgttatg ccgtaaatcc ttgtgagcat tttgttcttt
     3781 tcgttccctc tccagtatgc gccggctaca ttgaggagct ttattgcccg gatcggtgcg
     3841 gttgatggaa ggtgcggccc gcggcacaga tcggtaaaat tgccctgagt gtacaaagtt
     3901 atggagccat cttcaagctc accgatcagc tctgttttgt attcatctcc tttggcggta
     3961 aaaatatcca tggcttcctt tttgcttact tccctgcggg taaacttaag gttttcagca
     4021 gctagctcct tcatcttttt ctcaatggcc ggcagatcgg catcggtgat aacccgaccg
     4081 tcgcccgggt caacatcata gtaaaatccg ttctcgatgg cgggcccgat accgaatttg
     4141 atcccgggat aaattgattc aagggcttcc gccatcaggt gggctgatga atgccagaaa
     4201 gcatgcctgg caggttcgtc atcccattta tgaagcacaa gcgatgcatc ggcatatatc
     4261 ggcctggtca gatcccgtac ctcattgttc actgttgcgg catatacctc accggcaagc
     4321 ctggggctga tctgttccgc gatttcaagg ctgctgatgc ccggttcgaa ttccttcacc
     4381 gagccgtcag gaaaagttat cttgatcatt aatcgtgtca tttttattga ggtgcaaatt
     4441 taatgaaaat tattttcaaa ggcttttgcc aatcacgatc ttatttccgg aaagccatga
     4501 ttgatccgga cgaaatttgc ggtttgacgt tactgttggc accaaatttg aattcagcat
     4561 tatgtacaag caacaataaa caccatgaac aaagttttct acataacatt tatcctgccg
     4621 ctgatcctta tttcgtgcga atattcgcca agggcatttt tttctgcaga ccctggcgat
     4681 ccggtggtgg gagaggaggt gtggttcacc agcggatctg acaatgcagt tgagcatgag
     4741 tgggatttcg gcgacgggtt ttattcaacc gaaagaaatc cggtgcacaa attcatgtct
     4801 tcgggtaaat tcaccgtgac actgaaggta tggtcctcct cgggcctaag tgacgaagct
     4861 tccctcggaa taaatgtcct gatcccgacg cttctcgaaa tcgaggtcct ggagtattac
     4921 gaggagtatc ctgtcaaagg tgcaagcgtt atcctttatc cgactctcgg cgactgggag
     4981 aatgaaagga acatgacagg agaaggtatt accgattccg acgggaaggt gatctttgcc
     5041 ggcctgggca aaaaagtaca ttatgtcgat gtctgggaaa agaaccatga taattatgct
     5101 ctcaaggaag aggatatcgg atttattcgt actcctgaaa taattcccaa cagaataaac
     5161 aggttcattg cctgggtcga ttatgtcgat catggcaaag ccggccggcg ggacgccagc
     5221 gttgtcgtca ggaaacttga aaggaaaccc tctgaaaaga aacaacccga agcgtcagac
     5281 ggcacttccg gctgggaaga actttacaac cggcgtgtac ggtaattaag ccgatggtcc
     5341 gggatttccg gccccggtat ttgaacgcct cacggatcgc cttcagaacc agctttgaat
     5401 caggtttttc ctgtcgtgct ggaattcact cagcctgtag aagatttctg aattctctga
     5461 ttcgatcctg tattcccagt aagtggaaat ggttcccggt gacatcattg tccgtctgat
     5521 ataaagaggt gaccctttga caaaatcgca tctttcggaa aatgcggata ttttgccgta
     5581 ggtgctgtcc attgaagttt tgatgaagat cagatatggt tcgcccgctt tttcgggtat
     5641 gtgctggcgg taatcaacaa agttcccgac atattttctc gtgattatca gagaatcttc
     5701 ctgtacggat tgctttgttg tggcacatga agcagctact atcaacagca gaagcaataa
     5761 ttttttcatg tgaaaataaa tttttatact actgaagatt aagtggttca catggaatcc
     5821 acatgaattt tatcttcatg cttgtttgtc attttgtatc atgtgggttt cataccggta
     5881 tttctgattt ccgcttgtca cataacaacg taaaagggtt atgcttta
//
LOCUS       DAOOYG010000155         5781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_16031_length_5781_cov_7.189137, whole genome shotgun sequence.
ACCESSION   DAOOYG010000155 DAOOYG010000000
VERSION     DAOOYG010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16031_length_5781_cov_7.189137"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PK288_10470"
     CDS             <1..483
                     /locus_tag="PK288_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPM19393.1"
                     /translation="ISQWGNVETQYQRRADLIPNFVNTVKGAANFEQETLTRVIEARA
                     KATQVNIDPANMTAENMRQFQEAQGELSSALSRLLVVVEKYPELKATQNFRDLQVELE
                     GTENRISVERRKFNEVARTYNTHIKRFPQNFLAGIFGFEARPYFESAEGAERAPQVQF
                     "
     gene            497..961
                     /locus_tag="PK288_10475"
     CDS             497..961
                     /locus_tag="PK288_10475"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPM19394.1"
                     /translation="METRASTFFSKEQQERIREAVRTAENETSGEIRVHIETSFRGNV
                     LDRASWIFRRIGMHATENHNGVLFYLAVRNREFAILGDWGINSRVPENFWNEISEVVS
                     SHFKAGDFTEGLVEGIIMTGNKLREHFPHKSDDVNELSDEISFDEPDQPAII"
     gene            958..1785
                     /locus_tag="PK288_10480"
     CDS             958..1785
                     /locus_tag="PK288_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPM19395.1"
                     /translation="MSRRRFLVPAGQLLLLLIIIQCHDIFAREFPDKPFPPRLVNDFA
                     GLLTDREADALERKLVAYDDSTSTQISIVIVTDLHGYDRSDYAQRLAEEWGIGQKGLD
                     NGILILVKPKTSESTRGEVFIAPGYGLEGVVPDIVCARIIDNEILPAFRAGAYYIGLD
                     RATTALMSLASGEFPPDKYGKSHDDKPGSLAPVVIIIILIILMMIIRSNGGSNQKHLG
                     RKGLPLWILLSMMNSGSSSHNGSWGGFSGGGGFSRGGGGFGGFGGGSFGGGGAGGSW"
     gene            complement(1833..2858)
                     /locus_tag="PK288_10485"
     CDS             complement(1833..2858)
                     /locus_tag="PK288_10485"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPM19396.1"
                     /translation="MAEEELNEQQELFEHYRYVVDPGQSLLRIDKYLSSRIENTSRTR
                     IQNAANAGNILVNNSPVRPNYKVKPGDTVQIVLPVPPREIELIPEDLPINILYEDEDM
                     IVVNKEPGMVVHPAYGNYSGTLINALMWHFRELPLFSSGELRPGLVHRIDKNTSGLLV
                     VAKNEHALNRLSRQFYERTTDRRYVAIIWGVPAASEGTITGHIGRNLRDRKIMQVFPD
                     GSQGKPAITHYRVIEDFGYVSVIECRLETGRTHQIRAHFSYIRHPLFNDEEYGGDEIL
                     KGTTYAKYYQFVKNCFSLLPRQALHAKTLSLDHPVTGKRLVFDSELPADMQQVINKWR
                     NYVSGRG"
     gene            complement(2866..4722)
                     /locus_tag="PK288_10490"
     CDS             complement(2866..4722)
                     /locus_tag="PK288_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM19397.1"
                     /translation="MTKEKIIYIILLILVPLVSYSQELVTGLQVNPLVKADKYRSGST
                     KGDGDIVELPFIDDFSGESHLPDPRYWSDNEVFINNTYTDKQITKGVATFDAIDGNGR
                     LYETITPAGFEADHLTSQPINLNYPKTDNIWLSFYYQPGGLGDLPEPKDSLVLQFYAP
                     DEAQWFPVWKAAGGERQDRFKPVMIRIDHERYLKSGFRFRFINWASLNPDLSEPSIVG
                     NCDHWHVDYVYLNRNRNEADSIHRDVAFTTPLRSLLKTYEAMPWKQFRQVFLQEMEAS
                     IPIHYRNNDIIVRNVTRNFTIRDMHRGTLAHSFSAGARNIDPQTSSDYNAALIYTFNT
                     ANTDSALFLVTSYLITDGFDRKENDTIKYNQVFSNYFAYDDGTSEGGYGINGLGSRNA
                     MVALRFKSYMQDTLRAVSICFNDSYLKSNLRTFDIVVWNDINDRPGDIIYSFEGVMVE
                     QGKDINGFYTYVLPDGVMVDDVFYVGWKQRSETFLNAGFDINTPHRGRQFYWLNGQWY
                     QSMVNGSVMIRPITGPPVKPASSDDSVTEERKSVLRIWPNPANDFINIESEDLRLSRS
                     AFITIIDLTGRQIMKVPYAERIDVSMLRAGIYTMVTTINNRPAGNLRFIKTK"
     gene            complement(4727..5572)
                     /locus_tag="PK288_10495"
     CDS             complement(4727..5572)
                     /locus_tag="PK288_10495"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPM19398.1"
                     /translation="MSLKHFILSRTFLKHLGLAAVIFVATVMIILIWMNFYTRHGQSR
                     PVPDFFGLTQEQTAQLARKSKLRFQIIDSVYTSEVPRGCIAEQNPRPGFRVKKWRNIM
                     LTINAFNPEMVAMPNLVNLPKRQALALIESSGLEMGTPIYIPDISIDVVIKQLYNGKE
                     IMEGDSIQKGSIIDLVLGKGLSNQRTDVPDLVGLNLDAAKSRILGASLTLVTSIYDNT
                     ILTGEDSAKAFVYKQNPEYNIDATIQLGSSVYLWLTVDSAKLPSDSTRFVFDTIPELY
                     SGQSE"
     gene            complement(5592..>5781)
                     /locus_tag="PK288_10500"
     CDS             complement(5592..>5781)
                     /locus_tag="PK288_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HPM19399.1"
                     /translation="LSFGTDDLDGTIEDTTRIYSMAGAGEKNPAMTTEEICKLINDAG
                     FRPVERDSLYNFITTFAL"
BASE COUNT         1478 a         1292 c         1419 g         1592 t
ORIGIN      
        1 ataagtcaat ggggtaatgt ggaaacccag tatcagcgca gggctgatct tatccctaat
       61 tttgttaaca cggtcaaagg agctgccaac ttcgagcagg aaacccttac acgagtcatt
      121 gaagcaaggg ccaaagccac ccaggtaaac atcgatcctg caaatatgac ggctgagaac
      181 atgcggcagt ttcaggaggc ccagggtgag ttaagctcgg ctctttcgag actcctggtt
      241 gtcgtggaaa aataccctga actgaaggct acccagaatt tccgtgacct gcaggttgaa
      301 cttgagggta cagagaacag gatctcggtt gaacggagaa aattcaatga ggttgcccgc
      361 acatataata ctcatataaa aagatttccc cagaatttcc ttgcggggat tttcggattt
      421 gaagccaggc cctattttga atcggcggaa ggggccgaaa gagctccgca ggttcagttt
      481 taaacaggtt gtaaaaatgg aaaccagggc ctccacgttc ttttccaaag aacagcagga
      541 gcgtatccgc gaagctgtaa gaactgcgga aaacgaaact tccggtgaga taagagtaca
      601 tattgagacc agtttcaggg gaaatgttct cgacagggct tcatggatat tcaggaggat
      661 cggaatgcat gctactgaaa accataatgg tgttttgttt tatcttgcag tcaggaacag
      721 ggaatttgca atattgggag actggggtat caactcgagg gttcctgaaa atttctggaa
      781 tgaaatcagt gaagtagtca gcagtcactt caaggcggga gattttacgg aaggtcttgt
      841 tgagggtata attatgaccg ggaacaagct cagggagcat tttccgcata aaagtgatga
      901 tgtaaatgag ctgagcgatg agatctcatt tgatgaaccc gatcaaccgg ctattatatg
      961 agtaggcgaa gattcctggt tccggcaggt cagttgttgt tgcttctgat cataattcaa
     1021 tgccatgaca tttttgcccg ggaatttcct gacaagcctt ttcctcccag gctggttaat
     1081 gattttgcag gcctgctgac agaccgggag gcagatgctc ttgaacggaa gcttgtggcc
     1141 tatgatgatt ccacttccac acagatatca attgtaattg tgacggatct gcatggctac
     1201 gacaggtccg actatgccca gcgccttgcc gaggaatggg gaatcgggca aaagggcctg
     1261 gacaatggca tacttatcct tgtaaaacca aagacatcag aaagtaccag gggagaagta
     1321 ttcatagctc ccggatacgg actggaaggt gtcgtacctg atatcgtgtg tgcccggatc
     1381 attgataatg agatcttgcc ggcctttagg gccggagcct attatattgg actggacagg
     1441 gcaacaaccg cactcatgtc cctggccagc ggcgaatttc cccctgacaa gtatggaaaa
     1501 tcacatgacg acaaaccggg aagcctcgca ccagttgtga taataataat cctcataatt
     1561 ctcatgatga ttatccgcag taacggcggg tcgaatcaga agcatctggg ccgcaagggc
     1621 ctgccgttat ggatattgtt atcaatgatg aattcgggca gcagttccca caatggcagc
     1681 tggggaggat tttctggagg cggcggattc agcaggggtg gcggaggttt cggaggattc
     1741 gggggaggaa gcttcggcgg cggaggtgcc ggcggaagct ggtagaaaag gccaggaaag
     1801 ccatgttcga catccattat acctgacctg tttcagcccc tgccggaaac atagttcctc
     1861 catttattga tcacctgctg catgtccgca ggcagttcgg agtcaaatac cagtcttttg
     1921 cctgtcacag ggtgatcgag actgagtgtt tttgcatgca gggcctgcct tggaagtaaa
     1981 ctgaaacagt tttttacaaa ctgataatac ttggcataag tagttccttt cagtatttca
     2041 tccccgccgt attcttcgtc attgaacaga ggatgacgga tatagctgaa atgggctcgt
     2101 atctgatgcg tgcggcccgt ttccagcctg cattcaatca ctgaaacata accgaagtct
     2161 tcaattaccc tgtaatgcgt tattgccggc tttccctgac ttccgtcagg aaaaacctgc
     2221 ataattttcc ggtcacgcaa attcctgcct atatgacctg taattgtgcc ttctgatgca
     2281 gcaggaactc cccaaattat cgcaacatac ctacggtctg tggtcctttc atagaactgt
     2341 cttgataacc tgttgagagc atgctcattc tttgccacaa ccagaaggcc cgatgtgttt
     2401 ttatctatcc tgtgcaccaa acccgggcgg agttcacctg aactgaaaag tgggagttcc
     2461 ctgaaatgcc acatcagggc atttataagg gtccctgaat aatttccgta tgccggatgc
     2521 acaaccatcc ccggttcctt gttaaccact atcatgtctt catcttcata gaggatattt
     2581 attggcagat cttcagggat aagttcgatt tccctcggag gaaccgggag gactatctga
     2641 acggtgtcgc ccggcttgac tttataatta ggcctgacag gagagttgtt gacaagaata
     2701 ttgccggcat tggctgcatt ctggatcctg gtgcgcgatg tattctcaat tcttgaagaa
     2761 aggtacttgt caattctcag cagcgattga cccggatcga caacatatct gtaatgttcg
     2821 aacaattcct gctgttcatt aagctcctct tcagccatcc gggcattatt tggtttttat
     2881 gaatcgtaag tttcccgccg gcctgttgtt gatagttgtc accattgtgt aaatgccggc
     2941 cctgagcatc gacacatcga ttctttcggc gtagggaacc ttcattatct gccggcccgt
     3001 aagatcgatg atagttatga aggctgaacg cgaaagcctg agatcttcag attcaatgtt
     3061 tataaaatca tttgcaggat ttggccatat ccttaatacc gatttccttt cttctgtaac
     3121 ggaatcatcg gaagaagctg gcttgacggg agggcctgtg atgggtctta tcattacgct
     3181 gccgtttacc atggattggt accattgtcc gttcagccag taaaactgcc ttcccctgtg
     3241 aggtgtgttt atatcaaacc cggcattgag aaaagtttca gatcgttgtt tccagcccac
     3301 ataaaacaca tcatctacca taacaccgtc gggaagcaca taggtgtaaa acccgtttat
     3361 gtcttttccc tgttcgacca tcactccctc gaatgaatag attatatcac ccggcctgtc
     3421 attgatgtcg ttccatacaa cgatatcaaa tgttctcaga ttggacttca gataactgtc
     3481 attgaagcaa atgctgaccg cccggagagt atcctgcata taggatttga acctgagagc
     3541 caccatggca tttctggatc cgaggccgtt aattccgtaa cctccttcag aggtgccgtc
     3601 gtcgtatgca aaataattgc tgaaaacctg attgtatttt attgtgtcat tttctttacg
     3661 gtcaaatccg tctgtaatca ggtatgatgt gaccaggaaa agggcagaat cggtatttgc
     3721 agtattaaag gtataaatga gcgctgcatt gtagtctgaa cttgtctggg gatcgatgtt
     3781 tcttgccccg gcagagaagg aatgagcaag tgtgcccctg tgcatatcgc ggattgtgaa
     3841 attacgggtt acgttcctga caatgatgtc gttattccgg taatgaatcg gtattgaggc
     3901 ttccatttcc tgcaggaaaa cctgtctgaa ttgtttccag ggcatggctt cataggtctt
     3961 gagcaatgat ctcagtggcg ttgtaaaagc aacatcccta tggatagagt cggcttcatt
     4021 cctgttcctg ttgagataaa catagtcaac atgccagtga tcgcagttac cgacaatgga
     4081 aggctcgctg aggtcagggt tcaggctcgc ccagttgatg aatctgaatc tgaatccgct
     4141 tttcaggtac cgttcatggt cgatccggat catgaccggt ttaaacctgt cctgcctttc
     4201 cccgccggct gccttccaca ccggaaacca ctgtgcttca tccggggcat agaactgaag
     4261 caccaggctg tctttgggtt cgggcagatc tccaagaccg ccgggctggt aataaaagct
     4321 caaccatatg ttgtcagttt tcggataatt gagattaatt ggctgggagg tgagatgatc
     4381 cgcttcgaaa cctgccgggg ttattgtctc ataaagtcgc ccgtttccgt caatggcatc
     4441 aaatgtggcc accccttttg tgatttgctt gtcagtataa gtgttgttga tgaagacttc
     4501 attatcgctc cagtacctgg gatcgggcaa atgagattcc cctgagaagt cgtcgatgaa
     4561 cggcagctca acgatatccc cgtcaccctt ggtgctacct gaacggtact tgtccgcttt
     4621 tacgagtgga ttcacctgaa gaccggttac cagctcctgg gaatagctga ccagtggaac
     4681 aagaatcagc aggataatat aaatgatctt ctcttttgtc atcagcttac tctgactgtc
     4741 ctgaatacaa ttcaggaatg gtatcgaaca caaacctggt tgaatcggat gggagtttgg
     4801 ctgagtcaac agtcagccag aggtaaacgg atgaacccag ctgtattgtt gcatcaatgt
     4861 tatattcagg attctgtttg tatacaaacg cttttgcgga atcctcacct gtcagaatgg
     4921 tgttgtcata aattgaagtg acaagagtaa gagatgcccc gaggatcctg gatttcgcag
     4981 catcaaggtt cagtcccaca aggtcaggta catctgttct ctggttgctc aatcccttgc
     5041 caaggaccag atcaataatg gatccttttt gaattgagtc gccttccata atttcctttc
     5101 cgttgtaaag ctgtttgatc accacatcaa ttgaaatgtc gggtatgtag atgggagttc
     5161 ccatttcaag acctgaactt tcgataaggg caagtgcctg ccttttgggc aggttaacca
     5221 gatttggcat ggcaaccatt tcgggattga atgcattgat tgtcagcatt atgtttctcc
     5281 atttttttac cctgaagcct ggccggggat tctgttctgc gatgcagccg cgcggtactt
     5341 cagaggtata aacagagtcg ataatctgaa acctaagctt gctttttctg gcaagttgtg
     5401 ccgtctgttc ctgtgtgagg ccgaaaaaat caggtaccgg tctgctctga ccatgacggg
     5461 tatagaaatt catccatatc aggataatca ttacagtggc tacaaaaatt actgcagcaa
     5521 gtcccagatg tttcaggaag gtcctgctta aaataaagtg tttgagactc ataccgtgtc
     5581 ctggtgtaga tttataacgc aaatgtagtt ataaaattat aaagcgaatc cctttcaaca
     5641 ggcctgaatc cggcatcgtt tataagcttg cagatttctt ctgtagtcat tgcggggttc
     5701 ttttctccgg cgccggccat ggagtatatg cgtgtggtat cttcaattgt gccatcaagg
     5761 tcatcagtgc cgaatgaaag g
//
LOCUS       DAOOYG010000156         5732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_16217_length_5732_cov_5.242029, whole genome shotgun sequence.
ACCESSION   DAOOYG010000156 DAOOYG010000000
VERSION     DAOOYG010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16217_length_5732_cov_5.242029"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..736
                     /gene="ndhC"
                     /locus_tag="PK288_10505"
     CDS             386..736
                     /gene="ndhC"
                     /locus_tag="PK288_10505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPM19400.1"
                     /translation="MSNEWLVLFFLVGAIFVGLVLILSGIIAPRSVNPQKFEPYESGI
                     PTKGVTWLQFNVGYYLFAILFLVFDVETVLVFPWAVVMKEVGLTAFIEIVIFFLILGL
                     GLLYAWKKDALVWE"
     gene            706..1296
                     /locus_tag="PK288_10510"
     CDS             706..1296
                     /locus_tag="PK288_10510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HPM19401.1"
                     /translation="MEKRCTCMGIKGMRNEDFKDNESLDLLRESGANILLTSIDDVIN
                     WGRKNSLWPLTFATSCCGIEMMATGAPRHDFARFGMEVYRASPRQADVIVVAGTIVNK
                     MAPVLRRLYDQMSEPKYVVAMGACAISGGPFFLNSYSVVKGVEHVIPVDVYVPGCPPR
                     PEALLYGMLQLQRKIETETIRSRKKRSASHSEKKSQ"
     gene            1420..2897
                     /locus_tag="PK288_10515"
                     /pseudo
     CDS             1420..2897
                     /locus_tag="PK288_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245586.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
     gene            2959..4035
                     /gene="nuoH"
                     /locus_tag="PK288_10520"
     CDS             2959..4035
                     /gene="nuoH"
                     /locus_tag="PK288_10520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPM19402.1"
                     /translation="MYDFTSLTQWIDTSLNDRLSPFWAVVAEMAIVGLLILVFYALIG
                     LFLVYAERKVCAFMQNRLGPNRVGPFGIFQTVADLIKLLLVELVPIRNADKFLFNVAP
                     FIVIIASFMAISAIPFAKGLHAIDLNIGVFYVIAVSALGVIGVLLAGWSSNNKYSLIG
                     AMRSGAQIVSYELSVGLSLITVIILAGTMQLSEIVQAQSTGWFIFRGHIPAFIAFVIF
                     LIASTAETNRGPFDLAEAESELTAGFHTEYSGIKFAFFFLAEYMNMFIVASIAATVFL
                     GGWMPFHIGSWEAFNHVMDFIPPFIWYLMKTFFIIFLMMWFKWTFPRLRIDQLLTLEW
                     KYLLPINLVNVVFMAFIVLMGWHF"
     gene            4064..4543
                     /locus_tag="PK288_10525"
     CDS             4064..4543
                     /locus_tag="PK288_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM19403.1"
                     /translation="MRSIVKYISEVLSGVWSLLKGMKVTGSYFFRPSAIVTQKYPENR
                     KDLVMFERFKGEVIMPHNENNEHRCTGCGICEINCPNGTIEVITKTIITPEGKKKRAI
                     DKHIYRLSMCSFCALCVKTCPSNALAFSQEFEHAVFDRSKLIKISNKPGSQLMKGVE"
     gene            4561..5085
                     /locus_tag="PK288_10530"
     CDS             4561..5085
                     /locus_tag="PK288_10530"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPM19404.1"
                     /translation="MFILFASIIVVFSILTVTSRRILRAAVYLLFVLISTAGLYFMLN
                     YAFLAAVQLTLYAGGIVVLIIFSILLTSHISLKFEPVEWKKSLFSALAAIAGALITII
                     TILDFTFSATNEAAREVNMRLVGKSLLSVGSDGYILPFEVISVLLLAAMVAAIIIAKK
                     GGPKSTDTQTSNYQ"
     gene            5087..5404
                     /gene="nuoK"
                     /locus_tag="PK288_10535"
     CDS             5087..5404
                     /gene="nuoK"
                     /locus_tag="PK288_10535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPM19405.1"
                     /translation="MNAGIPLSYFLILAAVMFFTGIYGFLTRRNLITILMSVELILNS
                     VNINFLALNRYLFPHKLEGFFFSLFIITVAAAEAAVAIALIINIYRRFTTINVEDVNI
                     MKY"
     gene            5426..>5732
                     /locus_tag="PK288_10540"
     CDS             5426..>5732
                     /locus_tag="PK288_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPM19406.1"
                     /translation="MTDFNYTLWIFLIPFLVFILLGLAGHRLKARISGLLGTAALGVV
                     TVLSYITAYFYYFRTAGINDVFRKITVFDSTWLHFTDTLEIKMGILLDPISVMMLIVI
                     "
BASE COUNT         1593 a         1265 c         1318 g         1556 t
ORIGIN      
        1 ggaactgagg gaacagacag ccagcctaga aggcccaacc gttatcctgc ggtttgccag
       61 aagccttaat atccaggatc ttattgatgt tatggaactg ggtgtaagat taaatatcaa
      121 aatggtactg gcaaccgata aggcaggtaa ccagttgtaa tcatctgaag acatgtcagg
      181 agcaataccg ggcaaaatgc ctcccggtac accagaatca gcatccctga gtgtaaggac
      241 gccctttctt tcatccctat aaaaccgaaa ccctgggata aagctggtga tatttatcac
      301 ataattttaa tttctgattt tttcagattc aagcaaagaa attctatttt tggcctgctt
      361 tctaagggaa cctaactctg atctcatgag caacgaatgg cttgttttat ttttcctggt
      421 tggggcaata tttgttggcc tggtactgat tctctcagga ataattgctc caagatccgt
      481 caacccgcag aaatttgaac cctatgaatc cggtattccg acaaaggggg tcacctggct
      541 ccagttcaat gtcggatatt atctttttgc cattcttttt cttgtgtttg atgttgaaac
      601 tgtattggtt tttccgtggg ccgtggttat gaaagaagtt ggactgacgg ccttcataga
      661 aatagtaata tttttcctta tactgggcct gggattactc tatgcctgga aaaaagatgc
      721 acttgtatgg gaataaaagg catgaggaac gaggatttca aggataatga aagccttgat
      781 cttttacgtg aatcaggggc gaacatctta ttaacctcaa tagatgatgt catcaactgg
      841 ggaagaaaaa actcgctatg gcccctgaca tttgcgacca gctgctgcgg aatagaaatg
      901 atggcaaccg gggctccccg gcatgatttt gccaggtttg gcatggaagt gtatcgtgca
      961 agcccgagac aggcagatgt aattgtagtg gcaggcacaa ttgtcaacaa gatggctccc
     1021 gtactcagga ggctgtatga tcagatgtcg gagcccaagt atgttgttgc aatgggagcc
     1081 tgtgccattt ccggcggacc tttcttcctg aattcctatt ctgtcgtaaa aggagtggaa
     1141 catgttatcc cggtggatgt ttatgtaccc ggctgccctc ccaggccgga ggcgctgctg
     1201 tacggaatgc ttcagctcca gcggaaaatc gaaaccgaga caataagatc gaggaaaaag
     1261 cgcagcgcat cacacagcga aaaaaagtcg caataaacaa cagaagagat gtcagcagat
     1321 aactttaacc ttgaggaatc cataagatca tttgacgggg atgccattgt aaccgagggc
     1381 aaacagttta ccgaaataac ggttcatccc gaaaagctta tggcgctggc gatgtttctc
     1441 agggataatt ccgaaatggg ctttgatttt cctgcctgcg tgtcgggggt agattacggg
     1501 aatgacctgg gtgtggtgta tcacctccga tcaacaaaac ataaccggac aatagttgtc
     1561 aaaacaaggg tatccgacag gcagaatccg gtaatggaat ctgttactcc ggtctggccg
     1621 ggagcagaat accatgaacg ggaaatattc gaccttttcg gtatcagatt caccaatcat
     1681 cccgacctaa gaagattatt cctcgacaac tctgacggtt accccctgag aaaggattat
     1741 tccgacgatg taaatattgt caccaagtaa acatggagga acacttaaaa aattccgtta
     1801 ccgacggaca ggaggaatat gttctgaata taggacccca gcacccgtca acccacgggg
     1861 tgcttcgcct agttgcatca ctcaggggag agatcgttac caatatcgaa cctcatatag
     1921 gctatattca caggggcatt gaaaaaatgt gcgaaagcct gacctatccc cagatcattc
     1981 atcttaccga caggatggat tatctgtcgg cacatataaa caatgaggcc gtctgcctga
     2041 atgttgaaaa agcactggaa atcgatattc ccgacaggat caaggtaata cggaccatca
     2101 tggccgaact cacaaggatc cagtcacatc agcttttctg ggccaccttc gggatggacc
     2161 tcggagggct cacatgcttt ttctatggcc tccgcgaccg tgaaaaagtg cttgacattt
     2221 tcgaggaaac ctgcggagga cgcatgatag taagttataa ctgcccggga gggctgatgc
     2281 atgacataca tcccggtttt cagaaaaggg ttaaggagtt cattgaatat ttcaggaaaa
     2341 ccttgccgga atatgaccag ttgctgaccg gaaatgtaat attcagggaa aggaccatgg
     2401 gaataggtaa gctcaccctc ggggaagcga tagcccatgg catcaccgga ccaacagcaa
     2461 gggcctcggg gttttcatgt gatgtaagga aaaaggaacc ttacagtgca tacgacagga
     2521 tcaaattcaa tgaggtcctt ttcaccgagg gagataccta tcacagatac ctggtaagaa
     2581 tggatgaaat gagacagtcg atggatatta ttgaacagct cattgacaat ataccggaag
     2641 gaccgtatgc agttaagatg aaaccaataa tcagactgcc tgaaggagag tattatcaaa
     2701 gagttgagac cgcccgcggg gaacttggag tgtatattat cagcgacggc aacaagaatc
     2761 cgtacagggt caaattccgt tctccgaatt ttgtgaatct ttccgtgctt aatatgttat
     2821 ccaaaggatt caagatagct gaccttattg ccataagcgg ttctcttgac cttataatac
     2881 ctgacattga cagataatcc atgccggggc agcaagaatg cttaaggctg caaaaaatga
     2941 agtattatgc catttgacat ttatgatttt acatcactga cacaatggat cgataccagt
     3001 cttaacgacc ggctatcacc tttctgggcg gttgttgccg agatggccat tgtcggcctg
     3061 ctgatccttg tcttttatgc cctgatcgga ttgtttctcg tgtatgccga aagaaaggtt
     3121 tgtgcattca tgcagaaccg gctcggacca aacagggttg gaccgttcgg aattttccag
     3181 accgttgccg acctcattaa actgcttctg gttgaactcg taccaataag gaatgccgac
     3241 aaattcctct tcaatgttgc ccctttcatt gtgataattg cctcattcat ggcgatttcc
     3301 gccatcccgt ttgcaaaagg cctgcatgcg atcgatctta atattggcgt tttttatgta
     3361 atagccgtat cggcacttgg tgttatcggg gtactgctgg ccggctggtc gagcaacaat
     3421 aagtattcgc ttatcggggc aatgcgaagc ggagcccaga ttgtaagcta tgaactgtcg
     3481 gtaggactat ccctaatcac cgtcatcatt cttgccggta ccatgcaatt gtctgaaata
     3541 gtccaggcac agtcaaccgg atggttcatt ttcagaggac atattcctgc attcatcgca
     3601 ttcgttattt tcctgattgc aagcaccgcc gagacaaacc gcggaccttt cgacctggct
     3661 gaagcagaat ctgaacttac ggcaggcttc catactgaat attcgggtat aaaattcgca
     3721 tttttctttc tcgcagaata catgaacatg ttcattgttg catccattgc cgcaacggta
     3781 ttcctcggag gatggatgcc ctttcatatc ggcagctggg aagctttcaa ccatgtcatg
     3841 gatttcattc ccccctttat ctggtattta atgaaaacat tcttcattat attcctgatg
     3901 atgtggttca aatggacttt cccaaggctc cggatcgatc agctgcttac cctggaatgg
     3961 aagtatctgc ttcccataaa tcttgtgaat gtggttttta tggccttcat tgtgcttatg
     4021 ggatggcatt tttaatgaac tgataactga aagaagggtc agaatgagaa gtattgtcaa
     4081 atatataagt gaggttctca gcggtgtatg gtcgctgctg aaaggaatga aggtaacggg
     4141 ttcctatttc ttccggcctt ctgccatagt gacacagaaa taccctgaga accggaagga
     4201 tctcgtgatg ttcgaaaggt tcaagggcga agttataatg ccccataatg aaaataacga
     4261 gcacaggtgt accggatgcg gtatctgcga gattaactgc ccgaacggaa caatcgaagt
     4321 tatcacgaag actatcatta ctcctgaagg aaagaaaaaa agagccatcg acaaacatat
     4381 ataccggctc agcatgtgtt ctttctgtgc actgtgcgta aaaacctgcc cttcgaatgc
     4441 actcgccttt tcacaggaat ttgagcatgc ggtattcgac aggagcaaac tgatcaaaat
     4501 atccaacaaa ccgggatcac aattaatgaa aggagttgag taatgaccct gaatcagctg
     4561 atgtttatat tattcgcctc aattatcgtt gtattctcca tcctcacggt taccagcaga
     4621 aggatactca gggcagcggt ttacctgcta tttgttctga tttccaccgc gggcctttat
     4681 tttatgctga attacgcatt tctggccgcc gtacagctca ctttgtacgc cggaggtatt
     4741 gtggtactga taattttttc gattcttctg acgagccata tcagccttaa attcgaaccc
     4801 gtggaatgga agaaatcctt gttctcggcc ctggctgcaa tcgccggtgc cctaatcacg
     4861 ataattacca ttctcgattt cactttctca gcaacaaatg aagctgccag ggaagtcaat
     4921 atgaggctgg taggaaaatc gctcctctcg gtaggcagcg atggatatat tttgcctttc
     4981 gaggttattt ccgttctcct gcttgcagca atggttgcag caataattat tgcaaaaaaa
     5041 gggggcccca aaagcacaga tactcagaca tccaattatc aataaaatga atgcaggaat
     5101 tccattaagc tattttctga tcctggctgc agtaatgttt tttacaggca tatacgggtt
     5161 ccttacccgg agaaacctga tcaccatcct catgtcggtg gagctgatac ttaattcagt
     5221 caacataaat ttccttgcac tgaacagata cctgttccct cataagcttg aagggttctt
     5281 tttcagcctt tttataatta cagttgccgc ggcagaagca gcagtggcta tagcgctgat
     5341 aataaacata taccgaaggt tcaccactat caatgtggag gatgtcaaca taatgaaata
     5401 ctgataaaac aaagcaggca ctcctatgac agatttcaat tataccttgt ggatatttct
     5461 gattcccttc cttgtattca tcctgcttgg cctggcagga cacaggctca aagcccggat
     5521 atccggcctt ctgggaaccg ctgcactggg agtagttacg gtgctttcat acataacggc
     5581 atatttttat tatttccgga ctgccggaat aaatgatgta ttccggaaaa taaccgtctt
     5641 tgattcaaca tggctccatt ttactgacac acttgaaata aaaatgggaa tcctgctcga
     5701 tcccatttcg gtgatgatgc ttattgtcat ct
//
LOCUS       DAOOYG010000157         5694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_16361_length_5694_cov_5.337826, whole genome shotgun sequence.
ACCESSION   DAOOYG010000157 DAOOYG010000000
VERSION     DAOOYG010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16361_length_5694_cov_5.337826"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="PK288_10545"
     CDS             complement(<1..515)
                     /locus_tag="PK288_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19407.1"
                     /translation="MNIALISLFVVISIIAIIILTSSLKLHAFVALFVVSVALALAVL
                     PVGSIIKTIKEGFGATMGSIGFLIIFGAMIGITLDKTGATISIANYILSRTGVKRSAA
                     AIGITGFITGLPIFCDSGFIILSGLAKSFSIRSGTAISFMVTVLATSLYAVHCLIPPH
                     PGALAAAGIIDA"
     gene            complement(640..1875)
                     /locus_tag="PK288_10550"
     CDS             complement(640..1875)
                     /locus_tag="PK288_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPM19408.1"
                     /translation="MALNYIWIAFFLIGFTVALFKLIFTGNTHIFTDLVNAVFTNAKT
                     GFEISLGLTGALTLWMGLLKVGEKGGVVNILGRAVGPLFERLFPGLPKGHPAYGSIML
                     NIAANMLNLDNAATPVGLKAMKEMQDANPDKNTASNAQIMFLVLNASGLTIIPVSVIV
                     YRMQMGAANPADIFIPILISTFCALMTGLLSVAIIQKINLFDRVVLAYLGGLTAIVAG
                     IIVYFTRLPKDQISDVSTFAANFILISVITLFIVLALIKKVNVYDAFIEGAKEGFNIA
                     VRIIPFLVAMLVAIGIFRASGAMDFIISGIAGFFGWLGVDTKFTGALPVAFMKPLSGS
                     GARGLMVDAMATYGPDSFIGRLACTMQGTTDTTFYIIAVYFGSVGIKNTRYAIGCGLL
                     ADLAGFTAAILVAYLFFGQ"
     gene            complement(1911..2738)
                     /gene="proC"
                     /locus_tag="PK288_10555"
     CDS             complement(1911..2738)
                     /gene="proC"
                     /locus_tag="PK288_10555"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPM19409.1"
                     /translation="MKNMKIAILGGGNIGTSLAAGLIRSGICDYDTVIVSDKREARLS
                     YLRNKGFTVTSDNKEAIGFADHVILSVKPQQFEGLSDEIKDSAGRDKIIISTITGVVF
                     DDLEKRLGKRELIRIMPNTALEICESMTCISFRNTGSETEELIVALFDRLGKTMVIPE
                     DLMSAATVIGACGVAFALRFMRAMSQGGIEIGFNSDVSQIITAQTVKGAARLMLETRN
                     HPESEIDKVTTPQGITISGLNEMEHQGLSSAVIRGLTVSFNKLENMLSGSGSHNNLR"
     gene            3116..3778
                     /locus_tag="PK288_10560"
     CDS             3116..3778
                     /locus_tag="PK288_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPM19410.1"
                     /translation="MMENFNFPKLLAALTLVICMSSGLSGQDIVLPSPRKTGGKPLMQ
                     ALNERSTTRDFTKDELSLQQISGLLWAGFGINRPDIRKRTAPSAVNWQEIDIYVSMAS
                     GLYIYDAESNLLRQVHDRDIRSACGTQGFVAEAPLNLVYVADLTKCGKKEGDKVNDSD
                     LFMPYADCAFIGQNIYLFCASENLGCVIRGSIPKERLAKEMNLRPMQRILLSQTVGIP
                     SK"
     gene            complement(3801..5246)
                     /locus_tag="PK288_10565"
     CDS             complement(3801..5246)
                     /locus_tag="PK288_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPM19411.1"
                     /translation="MKKFIPILFAWLLTAGCSEVSPPEPFGPLPSDRQLAWHEKEYYM
                     FVHFTVNTFTDKEWGYGDEKEAVFNPTALDCRQWAKTAKEAGMKGIIITAKHHDGFCL
                     WPSAYTGHSVKNSPWRNGKGDILKDLRQACDEFGLELGVYLSPWDRNSAVYGTADYLT
                     YYRDQLRELLTDYGDIFEVWFDGANGGDGYYGGARETRRIDNSTYYDWPVTHSIVREL
                     QPMAVMFSDAGPDVRWVGNESGMGSLTNWCLLKKDGMYPGGDFARILGEGHEDGNYWV
                     PAEVDVSIRPGWFYHRSEDSLVRTPENLMELYYSSVGRNSNLLLNVPPDRRGLLNEID
                     VNSLLEFRKLREMEFADELARGKKAASSSDRGKNFRAALANDGDPDTYWTTRDDVTSA
                     SITIDLGTETGVNRILIQEYIRLGQRVKGYKVEVLKDDQWISVAEGTTVGYKVIRKFP
                     LVITKMVKLTILDSKACPVISNIELYRSPGE"
     gene            complement(5440..>5694)
                     /locus_tag="PK288_10570"
     CDS             complement(5440..>5694)
                     /locus_tag="PK288_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPM19412.1"
                     /translation="VIPAKRGYLRPVGEWNYQEVVAKGTRIKVTLNGTVILDGDIAGP
                     RDKGTMDKNDHPGLRNKTGHIGFLGHGSVVRFRNIRIKDL"
BASE COUNT         1513 a         1441 c         1281 g         1459 t
ORIGIN      
        1 gcatcgataa tccctgcagc ggccagtgcc cccgggtgag gcggtatcag gcaatgcact
       61 gcatacaggg atgttgcaag cacggtgacc atgaacgaaa tggcggtccc cgaccttatg
      121 ctgaaagatt ttgcaaggcc gctgagaata atgaatcccg aatcacagaa tatcggcagc
      181 ccggtaataa atccggtgat cccgattgcc gcggcgctcc gttttacgcc ggtcctggaa
      241 agtatataat ttgcaatgct tatggtcgca ccggtcttgt cgagggtgat gccgatcatt
      301 gccccgaaaa taataagaaa ccctatcgat cccattgttg caccgaatcc ctcctttatg
      361 gttttgatta tcgagccaac cggaagaacc gccagcgcga gagccacgga aacaacaaac
      421 aatgcaacaa acgcatgcag tttcaatgaa gaagtcagga taataattgc tattatactg
      481 atcaccacaa ataaagatat cagtgctatg ttcatgaccg gcagatgaag aaatatattt
      541 ctcttactga ttcagcaacc ctgccggaat tgaaaatctc ccttttcact gttaattcct
      601 taacaggaaa attcaatgcc ggcgtattgt catagaaatt cattgtccga agaacagata
      661 ggcaacaagg atcgctgcgg tgaaacccgc aaggtcggca agcagtccgc agccgatggc
      721 atacctggta tttttgatac caaccgatcc gaaataaact gctattatgt agaacgtcgt
      781 atcagttgtt ccctgcatgg tacaggcgag tcggcctatg aatgaatcag ggccgtaagt
      841 ggccatcgca tcgaccatca gaccccgggc cccgcttccg ctgagcggct tcatgaaggc
      901 aaccggtaaa gccccggtaa actttgtgtc aacaccgagc cagccaaaga aaccggcaat
      961 acctgaaatg atgaaatcca ttgctcccga ggccctgaaa ataccgattg ccacaagcat
     1021 ggccacaagg aaagggatga tccggaccgc aatgttgaat ccttcctttg caccctcaat
     1081 aaaagcatca tagacattga ctttttttat cagggctagt actatgaaaa gagtgattac
     1141 cgaaataagt ataaaatttg cggcaaaggt tgaaacatca gatatctggt ccttgggcag
     1201 cctggtaaaa taaactatga tgcctgcaac aattgccgtg agcccgccga gatatgccag
     1261 gacaaccctg tcgaaaaggt ttatcttctg aataattgcc acgctgagaa gcccggtcat
     1321 caaggcgcag aacgtggaga taaggattgg aatgaatatg tcggccggat tggctgcacc
     1381 catctgcatc ctgtaaacaa ttacacttac gggaattatt gtaagccccg aggcattgag
     1441 aacaaggaac attatctgcg cattcgatgc tgtattcttg tcgggatttg cgtcctgcat
     1501 ttccttcatt gccttgagtc ccaccggagt ggcagcattg tcgaggttca gcatgttggc
     1561 agctatgttg agcattatgg atccgtatgc tggatgtcct ttgggaagac ccggaaaaag
     1621 cctttcgaaa agcgggccaa cggcacgtcc gagaatgttg accacaccgc ctttttctcc
     1681 caccttaagc agacccatcc agagggtaag ggcacccgtc aggcccaggg aaatctcaaa
     1741 acctgttttg gcattagtaa acaccgcatt gaccagatcg gtgaaaatat gtgtgttgcc
     1801 ggtaaagatc agcttgaaaa gggccaccgt gaaaccaatg agaaaaaatg cgatccagat
     1861 gtaatttaag gccatttgaa cttaattgag catggatatt aatggaaatt tcatcgcagg
     1921 ttattatgac tgccggatcc cgaaagcatg ttttcaagtt tgttgaatga cactgtcagc
     1981 ccgcggataa cggcagaact aaggccctgg tgttccatct cattgagccc cgaaatggtt
     2041 attccctggg gtgtggttac cttgtctatc tcactttccg gatgattccg ggtctccagc
     2101 atcagccggg cagcgccctt aacggtttgt gcggtgataa tctgtgaaac atcagaattg
     2161 aaacctattt cgatcccgcc ctgcgacatg gccctcatga acctgagggc aaatgctacc
     2221 ccgcatgctc ctataaccgt tgctgcactc ataagatctt cgggtatcac catggttttt
     2281 ccaagccggt caaaaagcgc aactatcaat tcttcagttt cgctgcctgt attcctgaaa
     2341 gatatgcagg tcatagattc gcatatctca agtgcagtat tgggcatgat cctgatcaac
     2401 tccctcttcc ccaagcgctt ttcaaggtca tcgaatacaa cgcccgtaat ggttgatatg
     2461 atgatcttat ccctgccggc agaatccttt atctcgtcag acaaaccttc aaactgctgg
     2521 ggcttgaccg aaagtatcac atgatcagca aaaccaatcg cttccttgtt gtctgacgtt
     2581 accgtgaaac ccttgttccg caggtaactg agccttgcct cccgcttatc gctaacaata
     2641 acggtgtcgt agtcacatat tcctgacctg atcagtcctg cggcaagtga agtaccaata
     2701 tttcctcccc caagaattgc aatcttcata tttttcatgt aaaaataaat ttatatacta
     2761 gtgaagatta agtggttcac atggaaccca catgaatttt atttccatgc ttgtttgtga
     2821 ttttgtatca tgtgggtttc ataggcaaat gatttatttt ataggccgga tgcctgtccc
     2881 gctgaaggcg ggtgatccgc gcattgctga cccaggcagc tcctgcagat cccgctgata
     2941 atgataaaga tcctcatgta ttcatttctg aaaaaaatca tgcggatatc atttaaaaat
     3001 gaagttttcc gccgaaagat acttaaaatg gtaataaata acattcgtga aaagaaatga
     3061 tgtgacattt accggaataa attgtgatct ttgtttcata atatgaaaac aaaccatgat
     3121 ggaaaatttc aacttcccaa agcttttagc cgccctgacc ctggtaatct gcatgtcatc
     3181 ggggctttca ggccaggaca ttgttctgcc ttctccccgg aaaacaggag ggaaaccgct
     3241 gatgcaggca ttgaatgaaa gaagtacaac aagggacttt acaaaagatg aactttcact
     3301 gcagcagatc tccggtctcc tttgggcagg attcggcatc aaccgtccgg atatcaggaa
     3361 aaggacagcg ccgtcggccg taaactggca ggaaatagat atttatgttt ccatggcttc
     3421 cggtctttat atttatgacg ctgaatccaa cctcctcagg caggtgcacg acagggacat
     3481 caggagtgcg tgcggcaccc aggggtttgt tgctgaagcg cccctcaacc tggtgtatgt
     3541 tgccgatctg acaaaatgcg gaaaaaagga aggcgacaag gtaaatgatt ccgatctttt
     3601 catgccttat gccgactgcg cattcatcgg acagaacatc tatcttttct gcgcatcgga
     3661 gaacctgggt tgtgttatcc ggggcagcat accgaaggaa aggctcgcaa aggaaatgaa
     3721 cctgcggccc atgcagagga ttctgctttc acagacagtg ggaatccctt caaaataacc
     3781 tgcagaaact gccaccggta ttattcaccc ggcgaccggt aaagttcaat atttgaaatg
     3841 accggacagg cctttgagtc gagtatggtc agtttcacca tttttgtaat gaccagagga
     3901 aacttccgga tcaccttgta tcccaccgtc gtgccttcgg ccacggatat ccattggtcg
     3961 tccttcaata cttcaacctt atagcccttg acacgctgcc ccagtctgat atattcctga
     4021 atgaggatcc ggttgacgcc ggtttcagtg ccaaggtcaa ttgtgatgct tgccgaagtg
     4081 acatcatccc gggttgtcca gtatgtgtcc gggtcaccat cattggcgag ggcagccctg
     4141 aaattttttc ccctgtcaga agaagaggca gccttttttc ccctggcaag ctcgtctgca
     4201 aattccatct cgcgcaactt cctgaactca agaagtgaat ttacatctat ctcattaagc
     4261 aggcccctcc tgtcgggcgg cacattaagc agcaggttac tgttcctgcc aacggaggaa
     4321 taatacagtt ccatcaggtt ttcgggggtc cgtacaagtg agtcttccga tcggtgataa
     4381 aaccacccgg gccggattga gacatccact tctgcaggaa cccagtaatt cccgtcttca
     4441 tgaccttctc ccaggatcct tgcaaaatca ccacccggat acatcccgtc ctttttcaga
     4501 aggcaccagt tggtaaggct tcccatgccg ctttcattcc ccacccagcg tacatcgggg
     4561 ccggcatcgc tgaacatcac tgccatcggc tgcaattccc tgactatcga atgagtaacc
     4621 ggccagtcat aataggtact gttgtcgatc cgccgggttt ctctcgcgcc tccgtaataa
     4681 ccatctcccc cattggcccc gtcaaaccat acctcaaaaa tatccccgta gtccgtaagc
     4741 agttccctga gctgatccct gtaataggta aggtaatcag ctgttccgta aacagcgctg
     4801 ttcctgtccc atggtgagag ataaaccccg agttcgagtc caaactcatc acaggcctgc
     4861 cggagatctt tcagaatgtc tccctttccg ttcctccatg gtgaattttt taccgaatgc
     4921 cctgtgtatg ccgagggcca caaacaaaag ccgtcatgat gctttgcagt tatgattata
     4981 cccttcatgc ctgcttcctt tgctgttttt gcccattgcc ggcagtcaag cgccgtggga
     5041 ttgaaaactg cctccttttc atcgccatag ccccattcct tgtctgtgaa agtattaaca
     5101 gtgaaatgca caaacatgta atactccttt tcatgccatg caagctggcg gtcagacgga
     5161 agaggaccga aaggttcagg aggtgaaact tccgaacagc ctgcagtaag cagccaggca
     5221 aaaagaatag ggatgaactt tttcatgcga aaaaacaatg agaatttata cactgatcca
     5281 tcgttgaaac acggagcggc ggagctttaa aagaattctc tccgcggacc tccgtgcaac
     5341 aaaatatata attatgatct taaccgtccg gagctttccg cagttgtcag tccaagcaat
     5401 ttccaggtaa attgcccata gttccccggg attatggttc tataaatcct taattctgat
     5461 attacggaac cttacaacag atccgtggcc gagaaagcct atatgccctg tcttattccg
     5521 caggcccgga tggtcatttt tatccattgt tcccttatcc ctgggtcctg caatgtctcc
     5581 gtcaaggatc acggtaccgt taagggtcac ttttatcctt gtgcctttgg ctacaacctc
     5641 ctgataattc cattcaccca cgggcctgag gtaaccgcgt tttgcaggga tcac
//
LOCUS       DAOOYG010000158         5685 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_16404_length_5685_cov_7.176554, whole genome shotgun sequence.
ACCESSION   DAOOYG010000158 DAOOYG010000000
VERSION     DAOOYG010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16404_length_5685_cov_7.176554"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PK288_10575"
     CDS             complement(<1..1247)
                     /locus_tag="PK288_10575"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM19413.1"
                     /translation="MKKIIFISIIISALYMLNAIAQSPVVAPSQKTTIILSGGIIHTG
                     TGDVIDDGLISFSKGKITWVGKASEFKGDRTGADVIDVSGKHVYPGLIYPTTSLGLVE
                     ISGVDVTVDNRETGDLNPNVRSIVAYNTDSHVTPVVRSNGILIAQIVPTGTLLPGTSS
                     IVQLDAWNWKDAIYREDDGIMLGWPLKAPMTGRRGAYAIPQMPGREGESSYDRNVEQL
                     EKIFSDAVAYNAVSKPEDKNLRLEALKGLFEGTKTLFINAPEPKGIIAAISFAKKYGV
                     KKIVLTGADETAWTVKDFLKENNIPVILGHVHSLPEYEHSDTRLPFKLAAMFKKEGIL
                     VGLTYSNQAYGYNLPFVAGQTVAYGLTKEEALQTVTLNNAKILGIENVTGSLEVGKDA
                     NIVVSTGDILDMMTNNVEYAFING"
     gene            complement(1244..4249)
                     /locus_tag="PK288_10580"
     CDS             complement(1244..4249)
                     /locus_tag="PK288_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM19414.1"
                     /translation="MRKNIIFLLAFLPFYVFAQVDNAPVVGVSDKRVELYGLKNARVE
                     VDYQTTLENTDILISDGRIKSVGKGLTFPPGTIVYDLTGKTVYPSFIDVYAGNYGIKT
                     QTSGSESNPYAALMMQQMGGRQAQTATPEPRIADYWNDGINASYNVSEEFIPDSRTAG
                     EYRQIGFGAVVTFKADGIARGTSALVSTGEGKTNSLIIKNKASANYSFTRGRSADMYP
                     MAQFGIIALLRQLNYDAQWYKQLPEGYFHDDGLEAYISNSPLPQIFEVSKKIEIMRAD
                     KIGKEFNINYIIKGAGDEYQAVEDIKKAGNSLIIPVNYPEAPDVKDPYDAVTVPLSRL
                     KHYEMAPYNLSFVSKAGIPFAITSSDLRRRTDFLTNLRKAVKNGLPVDEALKALTATP
                     AAMIGATGLLGAVKNNMIANLLVTSGNIFEDNCIIYENWIQGKPYRLVDLRVKDIRGV
                     YDLKVDTVKYKLTVSGTFDKPTIKLVLDSTEIRGATLTRDKDLVLISFERMKVKFRLS
                     GYVADKNMEGEGQPDNGKWIKWIAEYTGTPPDAPRQTKPQPKTDIGQVIYPFTAYGYT
                     KIPGREDVLFRNATVWTNEKEGKLLKTDVLVKDGKISKIGKNLSASGARIIDATGMHL
                     TAGLIDEHSHIAMDGTNESGQAITSEVRVGDVVDPENQNIYRQLSGGVTAAHILHGSA
                     NPIGGQSVLIKHRWGHSAEELKIENQPGFLKHALGENVKRMASRYPNTRMGVDQIIRD
                     AYQRAVDYNNKWKSWNAMKPADRIGKIPPRRDLELDAIVDVLEKRSFIECHTYVQSEG
                     TMIMQLAKDFGLKVNSLIHFNEGYKVADQISEYGAGASVFADWWDYKYEVYEGINYNA
                     ALIMSQGVLTCLHSDDAEMGRRLNQEAAKTVKYGGVSETDALKLVTLNPAKLLHLDDR
                     MGSIKAGKDADLVLWTDNPLSVYARASKTMVDGVIYFDEELDSKLKQEIDSERNRIIA
                     NILRETPQQPAAGAPNVSGRPTIPNIPGL"
     gene            complement(4450..5676)
                     /locus_tag="PK288_10585"
     CDS             complement(4450..5676)
                     /locus_tag="PK288_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPM19415.1"
                     /translation="MKFRVKTMLVTFVTILIYSVSGTGQDPGRPAVPSARPAFRMPAR
                     IISPEIMPDNRVTFRIYSKDAQKVTVSGEWQSGNNAIETLVKNDTGLFSLTVGPLKPE
                     LYGYTFSVDGVNTIDPNNVQVRRDGARYQNFFIIPGPESDLYFHKNDVPHGTVTKIWY
                     KSDVLGFDRRCYVYTPAGYESGTKKYPVFYLLHGAGGDEDAWTNMGRTAQIMDNLIAQ
                     GKALPMIVVMTNGNANQAGAQNEVPPTTPQGGQGMAAYQRYAGKFEEHLVKDLVPFIE
                     KNFRTLTGMNNRAIAGLSMGGAHTQTITSNNPGVFSYIGVFSMGIMNMGPQNQDAAKV
                     EAERNAKLLVLKDSGYKLYWIACGKDDFVYKGVVTLRETLDKINFKYIYRESTGGHTW
                     ANWRIYLSEFAPMLFK"
BASE COUNT         1406 a         1375 c         1160 g         1744 t
ORIGIN      
        1 ccattaatga atgcatattc aacattattc gtcatcatgt cgagaatatc gcctgttgaa
       61 acgacgatat tggcatcctt tccgacttcc agcgaaccag taacattttc aataccaaga
      121 atttttgcat tattaagcgt gacggtctgc aatgcttcct cctttgtgag cccgtaagca
      181 acggtttgtc cggcaacgaa aggcaggtta tacccgtaag cctggtttga gtaggtcagc
      241 ccgacaagta taccctcctt tttgaacatt gctgcaagct tgaaaggaag cctcgtgtcg
      301 ctgtgctcat attcggggag ggaatgaaca tgtcccagta tgaccgggat attattttct
      361 ttcaggaaat cctttacggt ccaggctgtt tcgtcggctc ctgtgagaac aattttcttt
      421 acgccatatt tctttgcaaa tgaaatggct gcaatgatac ctttcggttc aggggcatta
      481 atgaaaagtg tcttcgttcc ttcaaacagt cccttcagag cttcaagtct cagattttta
      541 tcttcaggct tactaacagc attataagct acagcatctg aaaagatctt ttcaagctgc
      601 tccacattcc ggtcataact gctttctcct tccctgcctg gcatctgcgg tatggcatat
      661 gctcctctcc tgcctgtcat cggtgctttg agcggccatc ccagcatgat gccatcatct
      721 tccctgtaaa tcgcatcttt ccagttccag gcatccagct gaacaatact ggatgttcca
      781 ggcaacagtg ttcctgtcgg aacaatctgg gctatcagta tcccgttcga ccttacaacc
      841 ggcgtgacat gagaatcggt gttatatgct actattgacc ttacattcgg attaagatcc
      901 ccggtctccc tgttatcgac agtaacatcg acgccgctga tctccaccag acccagactt
      961 gttgtcggat aaatgagtcc gggataaaca tgtttacccg atacatcgat cacatcagct
     1021 ccggtccggt ctcctttaaa ttccgaagct ttaccgaccc aggtaatttt tcctttggaa
     1081 aatgaaataa gtccatcgtc aataacatcc ccggtacctg tatgaatgat acctcccgaa
     1141 agaataattg ttgttttctg tgaaggagca acaacggggc tctgtgcaat ggcattcagc
     1201 atatataatg ctgatataat gattgaaatg aatattatct tcttcataat cccggaatgt
     1261 taggtatagt tggacgtcct gatacatttg gagcacctgc agcaggttgc tggggagttt
     1321 cccttaggat atttgcaatt atcctgttgc gttcactgtc tatctcctgc ttcagctttg
     1381 aatcaagttc ctcatcgaaa tatataactc cgtctaccat tgtttttgag gctcttgcat
     1441 aaactgaaag aggattatcg gtccagagaa caaggtctgc atcttttcct gctttgatac
     1501 ttcccattct gtcatcaaga tgaagtaatt ttgccggatt aagtgttacc agcttcagtg
     1561 catccgtttc gctgacacct ccatatttga ctgtcttggc tgcttcctga tttagtctcc
     1621 gccccatctc ggcatcatcg gaatgaaggc aggtaagtac tccctgactc attataaggg
     1681 ctgcattgta atttatcccc tcatacactt catatttata atcccaccag tcggcaaaga
     1741 ctgacgcgcc ggcaccatat tctgatatct gatcggctac tttgtatcct tcattgaaat
     1801 gaataagtga gttcaccttc agtccgaaat cctttgcaag ctgcatgatc atggttccct
     1861 cagactggac atatgtatga cattcgataa aacttctttt ttcaaggaca tcaacaatgg
     1921 catccagttc cagatcgcgg cgcggaggga tttttccgat cctgtcggca ggtttcatgg
     1981 cattccagct cttccattta ttattataat ccactgctct ctgataagca tcgcggatta
     2041 tctgatcaac tcccatcctc gtattaggat aacggcttgc cattcttttt acattttcac
     2101 cgagagcatg tttcaggaac ccgggctggt tctcaatttt aagttcttca gcactatgcc
     2161 cccagcgatg cttaataagc accgactgac cccctatcgg atttgccgat ccgtgaagga
     2221 tatgtgctgc agttactcct ccggaaagct gacggtaaat attctggttc tcagggtcaa
     2281 ctacatcacc caccctgact tcagaggtta ttgcctgacc actttcgttg gtcccgtcca
     2341 ttgcaatatg tgagtgttca tctatcaggc cggcagtaag atgcataccg gtggcatcga
     2401 ttatcctggc tcctgatgca gacagatttt tcccgatctt cgagatcttg ccatccttca
     2461 caaggacatc tgttttcagg agctttccct ctttctcgtt agtccatact gttgcattcc
     2521 tgaaaagtac atcttccctc ccggggatct ttgtataacc ataggctgta aatggataaa
     2581 ttacctggcc gatgtcggtt ttaggttgtg gctttgtctg cctgggtgca tccggaggag
     2641 tacctgtata ttcagctatc cattttatcc actttccgtt atcaggttgc ccttcacctt
     2701 ccatgttctt gtctgcaaca taacctgaca gcctgaattt taccttcatt ctttcgaagc
     2761 ttatgaggac caggtcttta tcccttgtca atgttgcacc tcttatttcc gtggaatcaa
     2821 gaacaagttt gatcgttggt ttatcaaaag ttcccgaaac agtaagctta tatttaaccg
     2881 tatcgacctt taaatcataa acaccacgta tatctttgac tctcagatct accagcctgt
     2941 aaggctttcc ctgtatccag ttctcataga taatacaatt atcctcaaag atatttcctg
     3001 aggtgacaag caggtttgcg atcatattat tcttaacggc tcccagaagt ccggttgcac
     3061 cgatcatcgc ggccggggtt gctgtgagcg cttttaatgc ttcgtcaacg ggcaatccgt
     3121 tctttaccgc tttcctgaga tttgtaagga aatccgtacg gcggcgcaga tccgatgaag
     3181 taattgcaaa aggtatacct gcttttgata cgaacgaaag gttgtatgga gccatctcgt
     3241 aatgcttgag tcttgacaaa ggaaccgtga ctgcatcata aggatcttta acatcgggag
     3301 cttcaggata atttaccggg atgataaggc tgttgcctgc tttttttata tcctcaacag
     3361 cctggtattc atcacctgct cctttaatta tataatttat attgaactct ttgccgatct
     3421 tatccgctct cattatctca attttcttcg aaacttcaaa tatctgggga agcggggagt
     3481 ttgagatata tgcttccaga ccgtcatcat gaaaatatcc ctcaggaagc tgtttgtacc
     3541 actgtgcatc ataattaagc tgacggagca gggctattat tccaaactgt gccattgggt
     3601 acatatcagc cgatcttccc cttgtgaagg agtaatttgc tgaagcctta ttcttaatta
     3661 tcaggctgtt tgtttttcct tctcctgttg aaacaagggc agatgttcct ctggctattc
     3721 cgtcggcttt aaatgtaacg actgcaccga aacctatctg gcggtattcg ccggctgttc
     3781 tggaatcagg tataaattct tccgaaacat tgtacgaagc atttatgcca tcattccagt
     3841 agtcggcgat ccttggttcg ggagttgcag tctgcgcctg ccttcctccc atctgctgca
     3901 tcatcagggc agcatacgga ttggactcac ttcctgaagt ttgggtcttg atcccgtaat
     3961 ttcctgcata tacatcaata aatgaaggat aaacggtttt cccggtaagg tcataaacaa
     4021 tagtgccggg agggaaagtc agacctttgc caacggattt aatacgtccg tcagagataa
     4081 gaatatcggt attttcaagg gtagtctggt aatcgacctc cacacgggca ttcttcagtc
     4141 cgtagagctc aaccctttta tcgcttacac ccacaaccgg ggcattgtcc acctgagcga
     4201 agacataaaa aggaagaaaa gcaagaagaa aaatgatgtt ttttcgcata gattttattt
     4261 ggtttagagt ttcaggtttc aggttccaaa ttcaatgttc aaggatcaag gttcaagatt
     4321 caagattcaa gattctttgc cagccaaatc cgccaactgg ccgggaaggc tgggtttcag
     4381 ggttcgttac cggaaaccag acgccggacg ccggtagccg gtagccggta gccggtagcc
     4441 ggaatctcat tatttaaaaa gcattggtgc aaattccgat aaatatattc tccagttagc
     4501 ccatgtatga cctccggtac tttcgcggta aatatatttg aaatttattt tatccagagt
     4561 ttcccggagt gtaacgacgc ctttataaac aaaatcatcc ttgccgcagg cgatccagta
     4621 gagtttataa ccactatctt tgagaaccag gagtttggca ttcctttctg cttcaacctt
     4681 tgcagcatcc tgattttgcg ggcccatatt cattattccc atactgaata ccccgatata
     4741 actgaaaaca ccaggattat tgctggtgat cgtctgcgta tgagcgcctc ccattgacag
     4801 tcctgcaatt gcccggttat tcattccggt aagtgtcctg aaattctttt caataaacgg
     4861 gaccaaatcc ttcaccagat gttcttcgaa ttttcccgca tatctctggt aggcagccat
     4921 accctgtcct ccctgtggag ttgtcggagg tacttcattc tgtgcaccgg cctgattggc
     4981 atttccgtta gtcataacca cgatcatcgg taaggcttta ccctgggcga tcaggttatc
     5041 catgatctga gcagtccttc ccatatttgt ccatgcatcc tcatcgcctc ccgcaccgtg
     5101 aagaagataa aagacaggat atttttttgt tccgctctca tatcctgcgg gtgtataaac
     5161 ataacagcgt ctgtcaaaac ccaatacatc agatttatac cagattttgg taactgttcc
     5221 gtgaggcaca tcattcttat ggaaataaag atccgattcc ggtcccggga ttataaaaaa
     5281 gttctgatag cgggctccat cccttcttac ctgtacatta ttcggatcga ttgtatttac
     5341 accatcaacg gaaaaagtgt atccgtacag ttccggtttc aggggaccta ccgtaagcga
     5401 aaagagcccg gtgtcattct ttaccagtgt ttcgatggca ttattcccgg attgccactc
     5461 tcctgacact gtaactttct gtgcatcttt tgaataaatc ctgaaagtca ccctgttatc
     5521 aggcatgatt tcaggagata ttatccttgc gggcatcctg aatgccggtc tggcagatgg
     5581 tactgccggc cttcccggat cctgtccggt accggaaact gaataaatta aaattgtaac
     5641 aaaggttaca agcattgttt ttactctgaa tttcattttt tttga
//
LOCUS       DAOOYG010000159         5673 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_16451_length_5673_cov_6.321289, whole genome shotgun sequence.
ACCESSION   DAOOYG010000159 DAOOYG010000000
VERSION     DAOOYG010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16451_length_5673_cov_6.321289"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PK288_10590"
     CDS             <1..314
                     /locus_tag="PK288_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19416.1"
                     /translation="CLAVVYYLADVLKWQKWGSFFKVFGTNALFAFFMSGIWTKLILY
                     VIKIPSGNNTTNLYNWTYEKIFVPVAGNMNGSLLFAMTQMLLIWFLTLFLYRKKIFIR
                     V"
     gene            353..1399
                     /locus_tag="PK288_10595"
     CDS             353..1399
                     /locus_tag="PK288_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPM19417.1"
                     /translation="MEDNEIIGIVSAGYEKLFGATILKADPLPRSGSDRRYFRIETPG
                     KSVIGTYNANREENEAFIGFSGHFRQKSLPVPEIFGYLPDEYVYFQQDLGDDNLYTWL
                     HKKAPESLFDDETMTLYRKILDKLILFQTRGIEGLDLELCYPHKSFDRQSMMWDMNYF
                     KYMFLKLLAVPFNERRLEHDFDTLADYLLETGQDYFLYRDFQTANVMVIEGEPWFIDY
                     QGGRKGAPQYDVASLLYDAKIPMPDSSRIELLEYYIGRFCSGSEEEKYRFRGHYSGFS
                     MIRLMQALGAFGFRGLYEQKPTFTDSIVPGVGILNRIISHAEEHIRLPELFGTIRAIP
                     GSGLFKKLAEARKS"
     gene            1407..2123
                     /locus_tag="PK288_10600"
     CDS             1407..2123
                     /locus_tag="PK288_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPM19418.1"
                     /translation="MKAMIMAAGKGTRLGRITQTIPKVLVDINGKSLLQRAVETCSRF
                     GFDDIIINVHYLADQVEEEIQRLRRLGFRLSVSDERERLLENGGGLFKARDFFDNDPF
                     LVYNADIVTDLDLSALLRYHREKNGLATLAVRKREGSRFFLVNGDGMLKGWCNNATGE
                     KILAGDPGSAPLTEIGFSSIQILDPVIFSYMTEGIYTVVDLYLKLAPLHNIFTYRHDS
                     GYWFNVGTPENLEKVREYLA"
     gene            complement(2120..3565)
                     /locus_tag="PK288_10605"
     CDS             complement(2120..3565)
                     /locus_tag="PK288_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPM19419.1"
                     /translation="MKLVNPSSVLRILGNILRIESIAFLCCLPVAFIYKENPGPFIFS
                     ALISIVAAEGLFMLSGKRKMEKISTREGFLSVTLAWIFLTSFGTLPYLLSGAIHDFPA
                     AFFESASGFTTTGSTAVANVEGLPYSILFWRSFTHWIGGLGIIMLVIIIMPSLGMTGG
                     QLMNLESSLKSKIHPRTQGVGLRLLYVYLGLTITETLLLCLGEMNLFDSICHTFGSVA
                     TGGFSTKNTSLSGYSAYSQYIVMIFMFLSGISFVVFYYLVKLNFRKVRQDEEMWFYIL
                     TVLFFGTLAMSVLVANTTRSIEPAFREGFFQVISIITTTGYITTDYLLWPPAGHVILF
                     ILLFAGASTGSTTGSIKMARHLVVIKNIRNVMTKLIHPNVITQIKLNNKPLSDTANIS
                     MLSFITLYVFIFIIGTILLVILNIDLITAGSAVATSLGNAGPGFGSAGPMFHYGVFSS
                     PVKLILSFLMIIGRLEIITVFVLFSKSFWRL"
     gene            3719..4912
                     /locus_tag="PK288_10610"
     CDS             3719..4912
                     /locus_tag="PK288_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HPM19420.1"
                     /translation="MGKVMVIGAGGVASVAVQKMASLPDVFTEILLASRTKSKCDAIK
                     ARTGSKRISTEQIDADNVPELRKLIKGFGPDIVVNLALPYQDLHIMDACLAEGVSYLD
                     TANYEPPEEARFEYSWQWAYHDRYKNAGLTAILGCGFDPGVTSVFTAYAARHHFDELH
                     YLDIVDCNAGDHGKPFATNFNPEINIREVTQKGKYWENGKWIYTKPHEIRKVLTYPDI
                     GPRDSYLIYHEELESLVKNFPTLKRARFWMTFGQEYLTHLRVIQNIGMAGIEPVMFDG
                     REIIPIQFLKAVLPDPGELGEDYKGWTSIGCRIRGTKDGREKTYYVYNNCSHEAAYRE
                     TGAQAVSYTTGVPAAIGTMMYLGGIWKKPGVFNVEEFDPDPFMEQLNRLGLPWVELHD
                     TDLEL"
     gene            4936..>5673
                     /locus_tag="PK288_10615"
     CDS             4936..>5673
                     /locus_tag="PK288_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HPM19421.1"
                     /translation="MDYSRIPSPCYVIDEERLRKNLRLIRQVSDDSGAEIILAFKGFA
                     MWGVFPVVREYIRGASASSLSEARLCMEEMGSLAHTYSPVYKESEFEHILGFSSHVTF
                     NSLAQYDKYAGFLDGYPRKVSAGLRINPELPLTGYDIYNPCSPGSRMGIIAEDLIRHG
                     LPDGIEGLHFHVLFEADSYALEKVLNTIEEKFGNFLPRLKWINMGGGHLMTRTGYDTA
                     HLVKLLTDFRQRYGVHIILEPGSAFAWE"
BASE COUNT         1578 a         1236 c         1399 g         1460 t
ORIGIN      
        1 cctgcctggc tgttgtgtat tatttagccg atgttctcaa atggcaaaaa tggggttcat
       61 ttttcaaggt tttcggaacc aacgctcttt ttgcattttt catgtcagga atttggacga
      121 agctgatact ttatgtaatt aaaataccct cgggaaacaa tactacaaac ctgtataact
      181 ggacttatga gaaaattttc gtacctgttg ccggcaatat gaatggcagc ctgcttttcg
      241 ccatgaccca gatgcttctt atatggttcc tgacactgtt tctctacagg aagaaaatat
      301 tcatcagggt ttaattatcc agcctgaata caaaactgat aatatttaaa aaatggaaga
      361 taatgagata atagggatag tttctgccgg atacgaaaag ttgttcgggg caaccatctt
      421 gaaagcggac cctctgccca ggtcaggttc cgacaggagg tatttcagga tagaaacacc
      481 gggaaaaagt gttattggta catataacgc caacagggaa gaaaacgagg cctttattgg
      541 cttctccggt catttcaggc agaaatcttt acccgtgccc gagattttcg ggtatcttcc
      601 cgatgaatat gtttatttcc agcaggatct tggtgatgac aatctgtata catggcttca
      661 taaaaaggcg ccggaatcgt tgtttgacga tgaaaccatg accctttacc gtaaaatact
      721 cgataagctc attctttttc agacccgggg gatagaaggg cttgatctgg aactatgcta
      781 tccacacaaa agtttcgacc ggcaatcgat gatgtgggac atgaattatt ttaagtatat
      841 gtttctgaag cttcttgctg ttccgttcaa cgagcgccgc ctggaacatg attttgatac
      901 tcttgccgat tacctgcttg aaaccggcca ggattatttt ctctaccgcg attttcagac
      961 ggcgaatgta atggttattg agggcgaacc gtggtttatc gattaccagg gaggaaggaa
     1021 aggcgcaccg caatatgacg tggcttccct gttgtatgat gcaaaaatcc cgatgcccga
     1081 cagcagccgc attgagcttc ttgaatatta tataggccgg ttctgttcag gatcggagga
     1141 agaaaaatac aggttcaggg gacactacag cggcttcagc atgatacgcc ttatgcaggc
     1201 tctcggcgct ttcgggttca ggggattgta tgaacaaaaa cctactttca ccgacagtat
     1261 tgttccgggc gtgggtatcc tgaaccggat catcagtcat gccgaggaac atatcaggct
     1321 ccctgaactg tttggaacga tccgggccat acccggctcg ggattattta aaaaacttgc
     1381 agaagcaaga aaatcatgac ctgcggatga aagcaatgat aatggccgcc ggaaaaggaa
     1441 cacggctcgg gaggataacg caaaccattc ccaaagttct tgttgacata aacggtaaga
     1501 gccttcttca gagggccgtg gaaacctgtt cacggtttgg gtttgatgat attataataa
     1561 acgttcatta cctggccgat caggtggagg aagagattca gcggctgcga agacttgggt
     1621 tcagattgtc ggtttctgat gaaagggaga ggctccttga aaacggggga ggcttgttca
     1681 aggccaggga ttttttcgat aatgatcctt tccttgtata caatgccgat atcgtgaccg
     1741 atctcgatct ctctgcactt ctacgttacc accgggaaaa gaatggactt gcaacacttg
     1801 ccgtgcgaaa gcgagaaggc agcagatttt tccttgttaa cggcgatgga atgcttaaag
     1861 ggtggtgcaa taatgccacg ggagaaaaga tactggccgg cgatcccggt tccgctcccc
     1921 tgaccgaaat cggcttctca tcaattcaga tccttgatcc cgtaatattc agttatatga
     1981 ccgaggggat ctataccgta gtcgatctct atcttaaact ggcaccattg cacaatattt
     2041 ttacttacag gcacgattcc ggctactggt tcaatgtagg cacccctgag aaccttgaaa
     2101 aggtcaggga atatctggcc tagagcctcc agaacgattt tgagaacaat acaaatacag
     2161 tgattatttc aagcctacct atgatcatca ggaagctcag gatcagtttg accggggatg
     2221 aaaaaactcc gtaatggaac atgggcccgg ctgatccgaa gcccgggccg gcgtttccga
     2281 gggatgtggc cacggcgctt cctgcagtta tgaggtcaat gttaaggatt acaagaagga
     2341 ttgtgccaat aataaagatg aatacataaa gtgttataaa cgaaagcatt gaaatatttg
     2401 cagtgtcgga aaggggcttg ttattgagtt ttatctgcgt gatgacattg ggatggatca
     2461 gttttgtcat cacatttctt atgttcttta taaccacaag gtgccttgcc atttttatgc
     2521 tcccggtagt cgatccggta cttgccccgg caaacaggag tataaatagt atcacgtgtc
     2581 ctgccggggg ccacagcaga taatcggtgg tgatgtatcc tgttgtggta attattgaga
     2641 tgacctggaa aaagccttcc ctgaacgcgg gttcgattga tctggttgtg ttggcgacca
     2701 ggactgacat tgccagtgtt ccaaaaaaca gtacagttag aatatagaac cacatctctt
     2761 catcctgcct gactttcctg aagttgagct tcaccaggta atagaaaacc acgaaactta
     2821 tgcccgatag gaacataaag atcataacaa tatactggct gtaggctgaa tatcctgaaa
     2881 ggcttgtgtt tttggttgaa aaaccacctg ttgccacgga accgaaagta tggcaaatgc
     2941 tgtcgaacag gttcatttcg cccaggcata aaagcagtgt ctcggtaata gtcagaccaa
     3001 gataaacgta cagcagcctt agtcccacac cctgggtccg cggatggatt ttgcttttta
     3061 gcgatgattc cagattcatc agttgccctc ctgtcatccc gagcgacggc attatgatta
     3121 ttaccagcat gattataccc agtccgccaa tccagtgtgt aaagcttctc cagaacagta
     3181 tcgagtaagg caggccttcg acattggcca cggcagttga tcccgtagtt gtaaaacccg
     3241 aagcggattc aaagaatgct gcgggaaaat catgtatggc gccgctcaga aggtacggaa
     3301 gggtaccgaa tgatgtcagg aatatccatg caagggtcac tgaaagaaag ccttccctcg
     3361 tcgatatctt ttccatcttt ctttttcctg ataacatgaa gagtccttct gccgcgacga
     3421 tggatatgag tgctgagaat atgaacggac ccggattttc cttgtaaatg aatgcaaccg
     3481 gaagacagca tagaaatgca attgattcta tccgaagtat gttcccaagg atccgaagga
     3541 ccgacgaagg attgacaagt ttcatttgtg gaagaaatca gggataaata tagtcagaaa
     3601 taggattcaa agggaatccg gttaatgcgg aattctccga ataaaaggga tgtgcctgtt
     3661 gtttcgcgat atggcatata aattatatct ttggatcaga cataaaaaca ctgtgcaaat
     3721 gggaaaagta atggttatag gagcaggcgg cgttgcctct gtggctgttc agaaaatggc
     3781 ttctctcccg gatgtattca ctgagatact gctggcaagc cgtacaaaat caaaatgcga
     3841 tgcaattaag gccagaaccg gcagtaaaag gataagtacc gaacagattg atgccgacaa
     3901 cgttcctgaa ttaagaaagc ttatcaaagg cttcggcccc gatatcgttg taaatctggc
     3961 gcttccctac caggacctgc atatcatgga tgcctgcctt gccgaaggag tatcgtatct
     4021 tgacaccgca aactatgaac cccctgagga ggccaggttt gaatacagct ggcagtgggc
     4081 ttatcacgac aggtacaaga atgccggatt aacagcaata ctgggctgcg gattcgatcc
     4141 gggggtaacc tcggtattta ctgcctacgc ggcccggcac cactttgatg aacttcatta
     4201 tctcgatatt gttgattgca atgccggcga ccatggcaaa ccctttgcaa ccaatttcaa
     4261 tcccgagatc aatattcgcg aggttaccca gaaaggcaaa tactgggaga acggaaaatg
     4321 gatctacacc aagcctcacg agatcaggaa ggtgttaacc tatcccgaca taggtcccag
     4381 ggactcctat ctgatctatc atgaggagct cgaatcgctc gttaaaaatt ttcccacctt
     4441 gaaacgtgcc aggttctgga tgacgttcgg acaggaatat cttacacatc ttcgtgttat
     4501 tcagaatatt ggcatggccg gaatagagcc ggtgatgttt gatggacgcg agataatacc
     4561 cattcaattc cttaaagccg tactacctga tccgggggag ctgggcgagg attacaaggg
     4621 ctggacatca ataggctgca ggatcagggg aaccaaagac ggcagggaaa aaacatatta
     4681 tgtttataat aactgcagtc atgaagcagc atacagagag accggagcac aggctgtcag
     4741 ctataccacc ggtgtgccgg cagcaatagg tacaatgatg tacctgggcg gaatctggaa
     4801 gaagcccggt gtattcaatg ttgaagagtt tgatcccgat ccctttatgg agcagcttaa
     4861 caggcttgga ttaccatggg ttgagcttca tgatacggac cttgaacttt aacccccggg
     4921 ggtcattgta tgcctgtgga ttattcaagg ataccgtctc cgtgctatgt aatagacgaa
     4981 gagagactca ggaagaacct ccggctcatc cggcaggttt ctgatgattc cggagcagaa
     5041 atcattctgg cattcaaggg atttgcaatg tggggagtat tcccggttgt aagggagtac
     5101 atccggggag catcagcaag ttcactcagc gaggcaagac tctgtatgga ggaaatgggc
     5161 agccttgccc atacttactc accggtttac aaggagagtg aattcgaaca tatactggga
     5221 ttcagctccc atgttacttt caattccctg gcacaatacg acaaatatgc aggtttcctg
     5281 gacggatatc cccggaaagt gtcggcaggc ttgcggataa acccggaatt acccctgacc
     5341 ggttatgata tttacaatcc atgctcacct ggttcacgca tgggaatcat tgccgaagat
     5401 ttgatccggc acgggcttcc ggatggtatc gaaggacttc attttcacgt actttttgaa
     5461 gcagattcct atgcccttga aaaagttctg aataccattg aagagaaatt cggtaacttc
     5521 ctgccacggc tcaaatggat taatatgggc ggcggtcacc tgatgacacg gaccgggtac
     5581 gacacggccc acctcgtaaa gttgctaacc gatttcaggc aaaggtatgg ggtacatatc
     5641 attcttgaac cgggaagcgc atttgcctgg gaa
//
LOCUS       DAOOYG010000160         5597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_16796_length_5597_cov_5.039516, whole genome shotgun sequence.
ACCESSION   DAOOYG010000160 DAOOYG010000000
VERSION     DAOOYG010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16796_length_5597_cov_5.039516"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PK288_10620"
     CDS             <1..260
                     /locus_tag="PK288_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19422.1"
                     /translation="SDQLGALINNRIFTLLPYNNYAVNYELVLANAWFTGKAIYPDRF
                     RDIDIEEKTNEILRAFLGKDLYHKVISSAPAFRNLSKDDFR"
     gene            257..1327
                     /locus_tag="PK288_10625"
     CDS             257..1327
                     /locus_tag="PK288_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPM19423.1"
                     /translation="MTGKLVLEYNRYLRKRILFFLLVFLLLVIIVLISLVSGSADIGF
                     RSIIDLVAGRSSAFASQVLLKIRMPRIAGAFLAGTSLAISGAVFQSLLRNPLASPFTL
                     GISGASAFGAAFAIILLGAGTSYGGSNDVAVINNPYLVTISAFIWSLASVAVILLLSG
                     YRGSTPEIIILAGIIISSLFGAGISAIQYFSDNVQLASIVFWTFGDLGRATWSNCLII
                     ALVVFPVLLFFIHQSWNYKSLQSGDEYAKSLGVNVTRVRLTGMVVASLATAVVTSFYG
                     IIAFIGLVVPHIVRRIIGGDEQFLIPASAVSGGAFLLLSDTLARTIISPVILPVGILT
                     SFIGAPLFLFLLIKGMGKGFWS"
     gene            1341..2099
                     /locus_tag="PK288_10630"
     CDS             1341..2099
                     /locus_tag="PK288_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPM19424.1"
                     /translation="MKISIQDIEFSYNGVPALESISEKFGKGDFIALVGPNGSGKSTL
                     IKCLNGILKVHKGVVLIDEKAINSLTPNELAKEIAYVPQSENKKPVLTVFDIVLLGRK
                     PYINWKPSKHDLKITAEIINLLHLETLALKDFNKLSGGQQQIVYIARALAQEPDILLL
                     DEPTANLDIRHQVEVLEILKELARKGITIVIALHDINIAVRYASRIMMLKEGKVFASG
                     GKEIINEQNIENLYDIKVKIFREDSRIFIIPEGI"
     gene            2122..3228
                     /gene="tsaA"
                     /locus_tag="PK288_10635"
     CDS             2122..3228
                     /gene="tsaA"
                     /locus_tag="PK288_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPM19425.1"
                     /translation="MKSFEIKPVGYVSNEFNDPADRHGMKQKPSTITINDEYREALLN
                     IGDCEYLDIVFWFHKSDNSRLTGKIPSGAERGVFASRSPKRPNLIGVTTVKLLEYSEN
                     KLVVTGLDAINNTPVIDIKCCDTSLFASESELNAVHSSILRSDPRIEIRNNIAAGNTE
                     SLLVKAGQLHGHFCPGLAMGVMAAAFAMKELGADSDGMENLLAITETNNCFSDGVQFV
                     TGCSFGNNSLIFRDLGKTAFTLTRRDGNGIRICSRHESGERVHEAFPEFRKFYQSVVI
                     EQNRSAELVSQYTRVALQRAFGTLTIPFEKLFTVSRVKAEIPAYAPIHESVVCGICNE
                     SVMKSRTTQNGDSFTCFACGGKEYGILDGSGIHF"
     gene            complement(3331..3786)
                     /locus_tag="PK288_10640"
     CDS             complement(3331..3786)
                     /locus_tag="PK288_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19426.1"
                     /translation="MAFLLHPVHVSITTLTQEAESDCLKVMFRMYFDDFQLDYKLFHP
                     GFKPGTGSEDSDYPHDMLNKYFNDRVQIYINNKLLPGKLTDVAINEYEILMNFEFESN
                     AKPRSFRIRNQILTRIYSDQANMIYLNINRYEDAIKLTVDDTEAVIRLK"
     gene            complement(3851..4369)
                     /locus_tag="PK288_10645"
     CDS             complement(3851..4369)
                     /locus_tag="PK288_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19427.1"
                     /translation="MEGSLTEPGTNIMLKVLIISLLFITQPPPVTFTSIEYVYGTDSL
                     KAVVRLSNELFLRDYQQTIFDDLDLATLRSFRPFPADLANNYLNSKIVIYTGKRQVIG
                     KLLKMQEDGGDIEFSLLFRVDRKLKSITVRNTILTGLSSKVRNLTSVRGRNTESLIVF
                     TPELPEKTFPLR"
     gene            complement(4551..4976)
                     /locus_tag="PK288_10650"
     CDS             complement(4551..4976)
                     /locus_tag="PK288_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPM19428.1"
                     /translation="MAKSETVSTRWLGNMAFESEVNGHKIIIDAGTASGGENRGPRPK
                     LLMLTALAGCTGMDVAAILKKMRVDIDGLNVIVDGDLTEEHPKYFYRMHVIYEFTGKD
                     LPADKLQKAIDLSTEKYCGVSAVYRKAMEITSEIRILEP"
     gene            complement(4993..5280)
                     /locus_tag="PK288_10655"
     CDS             complement(4993..5280)
                     /locus_tag="PK288_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPM19429.1"
                     /translation="MKELQPINQLVVLDITESKNEQKTSSGIIIPDTAREKPKTAPVV
                     WMGVIENAEIKPGDIVLYKPFTGTEAEFENSKYLILPYTDILAKVVETEKI"
     gene            complement(5302..>5597)
                     /locus_tag="PK288_10660"
     CDS             complement(5302..>5597)
                     /locus_tag="PK288_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HPM19430.1"
                     /translation="LALIQNSLYRPDTESGNKPARTVTVYSTPACSWCNTLKAWLNKN
                     GIQYTDIDVSRDQAGAEAMVRRSGQQGVPQTDIDGQIVVGFDQPRLKELLKIQ"
BASE COUNT         1439 a         1345 c         1279 g         1534 t
ORIGIN      
        1 tgtcagatca gctcggcgca ttaatcaaca acaggatctt caccctttta ccctacaaca
       61 actacgctgt aaattatgaa ctggttctgg caaatgcatg gttcacaggc aaggccatat
      121 atcctgaccg gttcagggac atagacattg aagaaaagac caacgaaata ctcagggcat
      181 ttttgggtaa agatctttac cataaagtaa tttcatcagc accggctttc cggaacctat
      241 ccaaagacga tttccgatga caggcaaact ggttctcgaa tataacaggt atctcagaaa
      301 aaggatatta tttttcctgc tggtttttct tctgctcgtc attattgtat taatttcgct
      361 ggtgtcgggt tctgccgata tcggtttccg gagcatcatt gaccttgtgg ccggaagaag
      421 ctcagcattt gccagccagg tgctcctgaa aatcagaatg ccccgcattg ccggagcctt
      481 cctggccgga acgtcacttg ccatatcagg ggcagttttc cagagcctgc tgaggaaccc
      541 cctggcctcg ccgtttacat tgggcatctc tggcgcatca gcatttggcg cagcttttgc
      601 aataattctg ctcggcgccg gaaccagcta cggcgggtca aatgatgttg ccgttatcaa
      661 taatccttac ctggtaacca tttcagcttt tatctggagc ctcgccagcg ttgctgttat
      721 actgcttttg tcgggataca ggggatcaac ccccgaaatc atcattcttg cgggaataat
      781 tatatcttcg ctgttcgggg cgggtatttc ggcaattcag tatttttcag acaatgtcca
      841 gcttgcatcc atcgtgttct ggaccttcgg tgacctgggc agggcaactt ggagcaactg
      901 cctgataatt gccctggtgg tttttcccgt attgctgttc ttcatccatc agagctggaa
      961 ttacaagtca ctccagtcgg gagacgaata tgcaaaaagc cttggggtaa atgttacccg
     1021 ggtgcgcctg accggcatgg ttgtcgcttc gctcgcaacg gctgttgtga catctttcta
     1081 cggtattatt gctttcatcg gactggttgt accgcacatt gtgcggagga taatcggggg
     1141 cgatgagcag ttcctgattc cggcctcggc tgtttcaggg ggcgcgtttt tgcttctgtc
     1201 ggataccctg gccaggacaa ttatttcccc cgtcatcctt ccggtgggaa ttctgacctc
     1261 attcattggt gcaccactgt ttttgtttct gttgatcaaa ggcatgggta agggattctg
     1321 gtcataaaac cttttttatc atgaagatct ccattcagga tattgaattc agctataacg
     1381 gcgtgcctgc acttgaaagc attagtgaaa aattcgggaa aggggatttt attgctctgg
     1441 tgggcccgaa cgggtcaggc aaaagcaccc tgatcaagtg tctgaacgga atattgaaag
     1501 ttcacaaggg cgtcgtgctg atcgatgaaa aggcaatcaa ctcactcacc ccgaacgaac
     1561 tggcaaagga aatcgcctat gttccccaga gtgagaataa aaaaccggtt ctaacggtat
     1621 tcgacattgt gcttctgggc agaaaaccct acatcaactg gaaaccatcg aaacatgatc
     1681 ttaagattac ggcagaaatc atcaatctgc tgcatctcga aacccttgcc ctgaaggatt
     1741 tcaataagtt gagcggcgga cagcagcaga tcgtctatat tgcacgtgca cttgcccagg
     1801 aacccgatat cctgctgctc gacgaaccta ctgccaacct cgatatacgt caccaggttg
     1861 aagtacttga aatattgaaa gagctggccc ggaaaggcat cacaatcgtc attgccctgc
     1921 atgatataaa catagccgtc cgctatgctt cccgaataat gatgctcaag gaaggaaaag
     1981 tttttgcctc cggaggaaaa gagatcatta acgagcagaa tattgaaaat ctttatgaca
     2041 taaaggtgaa aatattcagg gaagacagcc ggatcttcat tatccctgaa ggaatataaa
     2101 aaatcacaaa atctttaaac aatgaaatca tttgagataa aacccgtcgg atacgtaagc
     2161 aatgagttca atgatcctgc cgaccggcat gggatgaaac agaaacccag tacaataacc
     2221 atcaatgacg aataccgcga agccctcctg aatatcgggg attgcgaata tctggatatt
     2281 gttttctggt ttcacaagtc ggacaactca cggctcacgg gcaagatacc ttccggtgcc
     2341 gagagagggg tcttcgcctc caggagtccg aaaaggccga acctgatcgg ggtcacaacg
     2401 gtaaagctcc ttgaatacag tgaaaacaaa ctggttgtta caggacttga tgcaatcaac
     2461 aataccccgg taatagatat aaagtgctgc gatacctcat tgtttgcatc cgaatcagaa
     2521 ttgaacgccg ttcattcttc aatcctcagg tcagatcccc ggatcgaaat aaggaacaac
     2581 attgcagcag gcaatactga atcacttctc gttaaggccg gccagctgca cggacatttc
     2641 tgcccgggtc ttgcaatggg tgtcatggct gctgcatttg ccatgaaaga actcggggct
     2701 gactcggatg gcatggagaa cctgctggcc ataaccgaaa caaacaactg tttctcggac
     2761 ggagttcagt tcgtaaccgg atgttcattc ggcaataatt cccttatatt cagggatctt
     2821 ggaaaaacag ccttcaccct taccagaagg gatggtaacg gtatccgcat atgttcgcgt
     2881 catgaatcgg gcgagagagt gcatgaagca tttcccgaat tccgtaaatt ctatcagtca
     2941 gttgtcatag aacaaaaccg cagcgccgaa ctagtctcgc aatatacaag ggttgccctt
     3001 caaagagcct tcggtacact gaccatccct ttcgaaaagc ttttcaccgt aagccgtgtg
     3061 aaagccgaaa taccagctta tgctccgata catgaaagcg ttgtatgcgg gatctgcaat
     3121 gagagcgtca tgaaatcgcg gaccacacaa aacggagact ccttcacctg ctttgcatgc
     3181 gggggaaagg aatacggtat cctcgatggc agcggtattc atttctgatt gaaccggtgc
     3241 tgccttgtta ttcaaaacac cgcggtatgg caaataccgc cgtagtacat tgtcaccgat
     3301 aatgtaaagc aggccccctg gcgatcagtg ttatttcagc ctgattactg cctcggtatc
     3361 atcaacggta agctttattg catcctcata tctgttgata ttgagatata tcatgtttgc
     3421 ctgatcgctg tagatacggg tgagtatctg atttctgatc ctgaagcttc tgggttttgc
     3481 atttgattca aattcaaaat tcatcaggat ctcatattca ttgattgcaa catctgtcag
     3541 ttttcccggc agcagcttat tgttgatata gatctgcacc ctgtcattga agtatttgtt
     3601 caacatgtca tggggatagt cagaatcttc tgaacctgta ccaggcttga acccgggatg
     3661 aaacagcttg taatccagct ggaaatcatc aaagtacatc ctgaacatta ccttcaggca
     3721 gtcactctct gcctcctggg tcaatgtggt gatggataca tgaaccggat gaagtaaaaa
     3781 ggccaaatgc agtatcagta tccttaacat gtgttaataa tataacagat gggcatttcg
     3841 ctgtagatat tcatcttaag gggaatgttt tttccgggag ctcaggtgtg aacacaatca
     3901 gtgattcggt attcctgccc cttacagaag taaggttcct gactttgctg gataatccgg
     3961 tcagaattgt atttctgact gtaatgcttt tcagtttcct gtcaacccgg aacaggaggc
     4021 tgaattcaat atccccgcca tcctcctgca ttttcagcag cttgcctatc acctgccttt
     4081 tgccggtgta tatcactatt ttggaattca ggtaattgtt tgccagatcg gcgggaaaag
     4141 gcctgaaact tctcagggtt gccagatcaa ggtcatcgaa gatggtctgc tggtaatcgc
     4201 gcagaaaaag ctcattgctc agcctcacaa cagccttcag tgaatccgtc ccgtatacgt
     4261 attcaatgct ggtgaatgtg acaggcggcg gctgagttat gaataacaat gatatgatca
     4321 atactttcag catgatattc gtacccggct cggttaaaga tccttccatt ctgctggcgg
     4381 atccggcagg gcctgcatag tccgtttaaa acaggcggat tacatgcaaa agtatgaatt
     4441 ataattgcct tttgctaccc ggaacaaacg ccgctgaatg tattctcctt ccggtggcac
     4501 attgcctaac ggcaacccgg ctgcccggta ccagtctgag ccggataagc ttacggctcc
     4561 agtatcctga tctccgaagt gatttccatt gccttcctgt aaaccgcact tacaccgcaa
     4621 tacttctccg ttgacaggtc aattgctttc tgaagcttgt cggcgggaag atcctttccg
     4681 gtaaattcat aaatgacatg catcctgtaa aagtacttcg gatgttcctc tgtcaaatcc
     4741 ccatccacga taacattcaa tccgtcgata tcaacgcgca tctttttcaa tatggcggca
     4801 acatccatcc cggtgcatcc cgcaagcgca gtaagcatga gaagcttcgg cctgggtccg
     4861 cggttttcgc ctcccgaagc agtcccggca tctatgataa ttttatggcc gtttacttct
     4921 gattcaaagg ccatgttccc gagccacctc gtggaaacgg tttcactttt tgccatacac
     4981 tgtcaggtat tgctatatct tttcagtctc aacaaccttg gcaaggatgt cggtataggg
     5041 caatatcagg tatttactgt tttcaaattc tgcctccgtt cctgtaaacg gcttgtagag
     5101 aacaatgtcg cccggcttta tttccgcatt ctcaataact cccatccaca caaccggggc
     5161 tgttttgggc ttctctctcg cagtgtcggg aataattatg cccgatgagg tcttctgttc
     5221 attcttcgat tcggtgatgt caagcaccac cagctggttt attggctgca attctttcat
     5281 gttcctgttc aattttctgg attattgtat tttaagtaat tctttcaaac gcggctgatc
     5341 aaagcccact acaatctgtc cgtcgatatc ggtctgggga actccctgct ggccggaacg
     5401 gcggaccata gcttctgcac cggcctgatc acgtgaaaca tcaatgtcgg tatattgaat
     5461 accgttttta tttagccatg ccttaagggt attgcaccac gaacaggcag gggtggaata
     5521 tactgttaca gtcctggcag gcttattccc cgattctgta tcaggtctgt aaaggctgtt
     5581 ctgtatcagg gcaagat
//
LOCUS       DAOOYG010000161         5382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_17689_length_5382_cov_5.602403, whole genome shotgun sequence.
ACCESSION   DAOOYG010000161 DAOOYG010000000
VERSION     DAOOYG010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17689_length_5382_cov_5.602403"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PK288_10665"
     CDS             complement(<1..955)
                     /locus_tag="PK288_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPM19431.1"
                     /translation="MKRQNKKTSLVSRRQFIGTAAAAATALSIVPVATRCKGPVHTET
                     PEKSAGLPDSRFGGVQIGTITYSWRDQPGGVENILKYCREAGISSIELMSDAIESWAG
                     KPPSPTELPGSLPPEAQARLDNLTKQYKSTPEQIRKSVDPPVWQMIVAMIVGTTAEQL
                     QWRLSVPVTKFEELRKMFNDAGVGIHIAKFSPAGWSDGEIDYAFKAAKALGAKGVTDE
                     SGVETAKRLGPFAEKHGMFAIMHNHYQFADKNFNVDEVLAASPAIMLNFDCGHYFGST
                     GLNPVDFIEKYHDRIFSLHLKDKTGPNTDPPNENQVWGQGEV"
     gene            complement(1339..2430)
                     /gene="thyA"
                     /locus_tag="PK288_10670"
     CDS             complement(1339..2430)
                     /gene="thyA"
                     /locus_tag="PK288_10670"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPM19432.1"
                     /translation="MSQQYLDHSRAILTSERSNFKGGSKGSGIISLFGYQNEYDLRKG
                     YPLLTTKKMFTASMFHETIWFLRGDTNIKYLEDNNCPIWRPDAFQANLPGLQKEGIFP
                     EGIVKYSADWDKAMQEYGQRIKEDAGFAERWGDAGPIYGKQWRRWEYYDHDRGMFVEI
                     DQVGQMIEGLKKNPTGKKHIVDSWNPGDTPKMSLPPCHVLYQATANEEGELELQLYQR
                     SCDQFLGVPFNIASYAALTHVIARGAGMIPKTFIHTFGDSHFYSSIGKRTLWHRDNFR
                     ELQKRVRNVADREEYLEVVEWINKNAPADGNEEKYDHVTAILEQMSREPRPMPRLHIA
                     DKPFDQLTVDDFVIENYDPHPPIRRSMAV"
     gene            complement(2601..3296)
                     /locus_tag="PK288_10675"
     CDS             complement(2601..3296)
                     /locus_tag="PK288_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPM19433.1"
                     /translation="MKTIFETDREFICDIQAPCFRDLSPEETGFIRAGKTQVLFRKGD
                     NLTKQGAFTSYVLFLAKGLAKQYLEGDSSKTFNLRIIRSGEFLGLSAVFSENKFGYSA
                     SALTDCQAFLIEKETIAKVIKQNGLFGFSLARRYCEQNNNLLSVIQDLNYKQMNGRLA
                     GTLLYLDSLRSEAPDIFQVLSRKDLADFAGISTESAVKLLKLFEKDGLIELDEKDVIL
                     KNHGSLSEISKRG"
     gene            3485..3841
                     /locus_tag="PK288_10680"
     CDS             3485..3841
                     /locus_tag="PK288_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPM19434.1"
                     /translation="MLYTNLKHLETAADLKRAISENENVMVICGRMGPMCIPVYGIAE
                     DLEDEYRHVKFYDMEFDNPEAHIIRSLPEVRGFMGIPFTIYYKEGRVVRATSSIQTME
                     QVRAILDKEFSPAVTA"
     gene            3844..4803
                     /locus_tag="PK288_10685"
     CDS             3844..4803
                     /locus_tag="PK288_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM19435.1"
                     /translation="MSAGNHYDAIVIGAGPAGLTAGIYLSRARMRTLILDEGIVGGQM
                     VLTHEIANYPGVESISGYQLSGIMKKQARNFGCDIKANISIKDLELTGTLKHIILSDG
                     TEYTAGAVILSPGGRPRTLGVKGEDEFKGRGISYCATCDGDFFTGREIIVVGGGNSAL
                     EEAVSLTKYASKVTVIHQFDHFQAFEHAIGEARRNPKISFVMESAVTGFYGGEKLEGV
                     EITDLRTGGTRNLRTDGAFIFIGYVPNTEFLRGKVGLNQSGEIIVDAVMSTNIEGVYA
                     AGDSNAKRYRQVTIATGDGTIAALAATAYINELKIKHRQPAET"
     gene            4880..5224
                     /gene="trxA"
                     /locus_tag="PK288_10690"
     CDS             4880..5224
                     /gene="trxA"
                     /locus_tag="PK288_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPM19436.1"
                     /translation="MKNIPVVEDHENILTLTDKNFQHQIRNRVVLVDFWAGWCVPCRM
                     MAPVLNEIAGELSGNACVGKVDVEKYQGLAQRFRVRNIPTMVLFRNGKEVDRFVGVKT
                     KEFLMSRISKAS"
BASE COUNT         1356 a         1179 c         1323 g         1524 t
ORIGIN      
        1 gtacttctcc ctggccccat acctggtttt cattcggggg atcggtattt gggcctgttt
       61 tgtctttcag gtgcaggctg aaaatcctgt catggtactt ttcaatgaaa tccaccgggt
      121 tcagacctgt cgagccaaaa taatgaccgc agtcgaaatt cagcatgatt gcaggtgatg
      181 ccgcaagcac ctcatcgaca ttgaaattct tgtctgcaaa ctggtagtga ttatgcatta
      241 tggcaaacat tccatgtttt tcggcgaacg gtcccagccg ttttgcagtt tccactcctg
      301 attcatcggt gacacctttt gctcccagtg cctttgcagc tttaaaggca taatcaattt
      361 ccccgtcaga ccatccggcc ggagaaaatt tggctatatg tattccgaca cccgcgtcat
      421 tgaacatttt tctcagttcc tcaaatttcg tgacaggtac ggataagcgc cattgtaatt
      481 gttccgcggt tgtacccacg atcatggcga cgatcatctg ccagaccggc ggatcaacgc
      541 ttttccttat ctgttcaggt gtcgatttgt actgtttggt caggttatcc agcctggcct
      601 gtgcttcggg cggcaatgaa ccgggcagct ccgtgggact cggaggtttt cctgcccatg
      661 actcaatggc atcactcata agctcgatgg aacttattcc ggcttccctg cagtatttca
      721 ggatgttttc cacaccaccc ggctgatccc gccagctgta tgttatagtt cctatctgaa
      781 ccccgccaaa tctggagtcg ggcagaccgg ctgatttttc aggagtttca gtatgtacag
      841 gtcccttgca acgggttgcg accggtacta ttgataaagc ggttgcggcg gctgcagcag
      901 ttccgatgaa ctgacgccgt gataccagac tggttttttt gttctgtctt ttcattgtgg
      961 agaatttaga ttaaacatga catgatgccc cggtctttca gccataatac tgaaacatca
     1021 gtgttattcc cctctcccga ttcccggcgg tcatgtcaaa attaataaaa gcatatgtca
     1081 aataatgaca ccgcgcaaaa aaaatccatc gggagattac cggccggctg atcttcctga
     1141 ataattatgc ggataaggac agattcagca tggaagtagg aaattttcag aatgattcaa
     1201 aaccgttttt gcgtttggca tgcatgataa tccatcttat ctgcaggagc atggcagcga
     1261 ggcggccgga tctgaaagaa cgggccgctt aagatgtgat ccgtaaattt atgatgatat
     1321 tgtcccggca atttctgcct atacagccat actccttctg atgggaggat ggggatcata
     1381 gttttctata acaaagtcat caactgtaag ctgatcgaat ggtttgtccg caatgtgcag
     1441 acggggcatt ggcctgggtt cgcgcgacat ttgttcgagg atggcagtta catggtcata
     1501 tttttcctcg ttcccgtcgg cgggtgcatt tttatttatc cattcaacca cttcaaggta
     1561 ttcttccctg tctgccacat tccttaccct tttttgcagt tccctgaaat tatccctgtg
     1621 ccagagggtt ctttttccga tgctggagta aaagtgcgaa tctccgaaag tatgaatgaa
     1681 agttttgggt atcatccctg ctccacgggc aattacatgt gtaagagcag catagctggc
     1741 tatattgaaa ggaacaccca ggaattggtc gcagcttctc tgataaagct gcagttccag
     1801 ttcgccttct tcattggcag ttgcctggta gaggacatga cacggcggca atgacatttt
     1861 cggtgtatca cccgggttcc atgaatcgac gatatgcttt ttcccggtcg gattcttttt
     1921 cagcccttct atcatttgcc ctacctgatc tatttcgaca aacattcccc tgtcatgatc
     1981 ataatattcc cacctccgcc attgtttccc gtagatgggt ccggcatcgc cccatctttc
     2041 ggcaaatcca gcatcttcct ttatcctctg gccatattcc tgcattgcct tgtcccagtc
     2101 ggcagagtac tttacaatac cttcagggaa aattccttcc ttctgcagtc ccggcaggtt
     2161 ggcctgaaag gcatcaggcc tccagatggg acagttattg tcttcaagat atttgatatt
     2221 cgtgtcaccc ctcaggaacc atatcgtttc atggaacatc gatgcggtga acatcttttt
     2281 cgttgtcagc agcgggtaac cctttctcag atcatattcg ttctgatacc caaataatga
     2341 tatgatgcct gagcctttac tgcctccctt gaagtttgaa cgctcagatg tcaggattgc
     2401 cctggagtga tctaaatact gttgcgacat ttttgttcct gtttttggat tattttgcag
     2461 tcaaaaatag tctttatttc tctcgggtac catgctccgg gctgcctggc ggggtgaaaa
     2521 ttatcaacac cggttgttgg tagccgcagc ccggccgcag ccggcccggg cggttaaggt
     2581 tagatgaaat tacctgcgga ttaacccctt ttacttattt ccgacagcga accgtggttt
     2641 ttcaggataa catccttttc atccagttcg ataagtccgt ccttttcaaa aagcttgagg
     2701 agcttcactg cactttcagt cgatataccg gcgaaatcag ccaggtcctt tcttgaaagg
     2761 acctggaaga tatcaggagc ttcagatctg agagaatcaa ggtacagcaa tgtcccggca
     2821 aggcggccgt tcatctgttt atagttgaga tcctgtataa ctgacaggag attgttattt
     2881 tgttcacaat accttctggc caggctgaaa ccgaacaggc cgttttgttt tatcactttt
     2941 gcaatggttt ctttttcaat aaggaaggcc tggcaatcgg tgagggccga tgcggaatat
     3001 ccaaatttat tttcagaaaa tactgccgac agcccaagga attctcccga tcttatgatc
     3061 ctgaggttaa aggtttttga gctgtcgcct tcgaggtatt gttttgcaag gcctttggca
     3121 agaaataata cataggaggt aaaagcaccc tgctttgtca ggttgtcgcc ctttctgaaa
     3181 agcacctgtg ttttccctgc cctgatgaat ccggtctctt cggggctaag atccctgaaa
     3241 cacggtgcct ggatatcgca tataaattcc ctgtctgtct cgaatatggt cttcatgttt
     3301 taatgatctg atccataaat gccgcctgac cggcaaacgt atcttaccgg gcacaaagtt
     3361 aatccaaatc aattaaaaag ttgttctgtt tcaacccggg gattgtcatc cgcattggta
     3421 attttcttta aggatatcct gtgggagtgt atttttgcca cacgaatttt aaaactgaaa
     3481 agtaatgtta tacacgaatc tgaaacatct tgaaacggca gccgatctga aaagggccat
     3541 aagtgagaat gagaatgtta tggtaatctg cgggcgcatg ggtccgatgt gtatcccggt
     3601 ttatggaata gctgaagatc ttgaggatga atacaggcat gttaaatttt acgatatgga
     3661 atttgacaat cctgaagcgc atattataag gtcgcttcct gaagtcaggg gatttatggg
     3721 aattcctttc actatttatt acaaggaggg cagggttgta agggcgacat caagcatcca
     3781 gaccatggaa caggttaggg ccattctcga caaggagttt tcacctgcag taacagcctg
     3841 aatatgagtg ctggtaatca ttacgatgca atagtaatcg gagccggacc ggctggtctc
     3901 accgcgggca tttatctgtc gagggcccgt atgagaacgc tgattcttga tgaaggtatt
     3961 gtcggtggtc agatggtgct tacacatgaa atagccaatt atcccggagt tgaaagtata
     4021 agcggatacc agctttcggg gataatgaaa aaacaggcca ggaatttcgg atgcgatata
     4081 aaggcaaata tttcaataaa ggatcttgag ctcacaggta ccttaaagca tattatactg
     4141 tcggacggaa cagaatacac ggccggagcg gttatcctgt ctcccggagg aaggcccagg
     4201 acactggggg ttaagggaga agatgagttc aagggacggg gaatatcata ttgtgctaca
     4261 tgcgatggtg attttttcac aggcagggag atcattgtgg taggaggggg aaattctgct
     4321 ctcgaagaag cagtgtcact tacgaaatat gcttcaaaag tgaccgttat tcatcagttt
     4381 gaccatttcc aggcgtttga acatgcgatc ggggaagctc gcaggaaccc gaagataagt
     4441 ttcgtgatgg agtcggctgt tacgggtttt tatggcgggg aaaaactgga aggagtggaa
     4501 ataacggatc tcagaacggg cggaacaagg aatttaagga ctgatggtgc tttcatcttc
     4561 ataggttatg ttcccaacac cgaattcctc agggggaagg taggcctgaa ccagtcgggt
     4621 gaaatcattg tcgatgccgt catgtcgacg aatattgaag gagtgtatgc tgccggcgac
     4681 agtaatgcca agcgttacag gcaggtgacg attgcaacgg gcgacgggac aatagccgcc
     4741 cttgccgcca cagcatatat caatgagttg aaaataaaac ataggcaacc agcggaaact
     4801 tgatatgaat acaatactga ttatcagcgg gattttggtt ctcttacttg tatccctgta
     4861 tttttttgca aggaggaaaa tgaaaaatat tccggttgtg gaggaccatg aaaatattct
     4921 tacacttacc gacaagaatt ttcagcatca gataaggaac agggttgttc ttgttgattt
     4981 ctgggcagga tggtgtgtac cgtgcaggat gatggcgccc gtgctcaatg aaattgcggg
     5041 agaactcagc ggcaatgcct gtgtcggaaa ggttgatgtc gaaaaatacc agggtctggc
     5101 acagaggttc agggtaagaa acattccgac catggtgctt ttcaggaacg gaaaggaggt
     5161 tgaccgtttt gtcggggtca agacgaaaga atttttaatg agccggatca gcaaagcgtc
     5221 ttaaatggaa aaagtgttgt catatcgtga atttgtttcc ggtttaccgg ctgaaggcaa
     5281 atgttttctc ctgctttaca ggggaggaag tgaacagagt gcatgcgcct gtaagaatgt
     5341 ggggaaggtg gcggagggaa acgacaaaat caggttcttt tc
//
LOCUS       DAOOYG010000162         5345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_17853_length_5345_cov_6.204726, whole genome shotgun sequence.
ACCESSION   DAOOYG010000162 DAOOYG010000000
VERSION     DAOOYG010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17853_length_5345_cov_6.204726"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PK288_10695"
     CDS             <1..718
                     /locus_tag="PK288_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HPM19437.1"
                     /translation="KVSIPIFEHVGVNKQIKALFGKTVSFKHGAYLIIEHTEAFHVID
                     VNSGNRSRSGNDQESNALEVNMEAAREIARQLRLRDMGGIIVVDFIDMQSSENKQLLF
                     DRMKEVMANDRTKHNILPLSKFGLMQITRQRVRPEMNIVTDEKCPSCQGTGKSRPSIL
                     FVDEIERALSFIVERIKTRKIILNVHPFVAAYLRCGFFPIYRKWNLKYKILIKIQAAT
                     SYHALEYHFYDREGNEIDLA"
     gene            802..2793
                     /locus_tag="PK288_10700"
     CDS             802..2793
                     /locus_tag="PK288_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPM19438.1"
                     /translation="MNRKLQFLLFLLVILSRGLSAQIYDPVSWEFSYEKKGNNRYDLI
                     FAATIEEGSHIYSMDVPENGPIPTSFIFDTMPGYKLEDSVFEVTKPEDLFDEAFGFRI
                     KSFSNRAEFRQTVYSEEPAFTVSGTVNYMSCNKSACSPPKDVEFSIRIGSPVSFPGSS
                     AAAGTSGTGRPAGPGSGLLKFFLISVLAGFAGILTPCVFPMIPITVAFFSRGSENRVQ
                     SVIKAIIFGLSILLIYSLLGIIVSLTSAGAGFANTLSTHWIPNLIFFVLFILFAISFF
                     GAFEIVLPNRWVTGADSKVDRRGLLASFFMGLTTVIVSFSCTGPIIGVLLVEAATGDW
                     LRPTIGMSGFGLAFALPFTVFALFPSMMSRLPKSGGWLNSVKVFLAFIMLAFSLKFLM
                     TIDSVYNLRILTRDVYLAIWIVIFSLLGFYLLGKIKFPHDADTPHLGIFRLFLVIAVF
                     SFVIYLIPGLFGAPLKGLSSLLPSPQTSEFRHLGTVGNIAEPAGRQVPVFSNGPCSDV
                     KHGDIFDMPLGLNGYFDFEQGLTCASQQGKPVLLDFKGHACANCKNMEAKVWSDPEVL
                     QRLRDNFVIVALYVDDRTQLPENEWITSGIDGKVKKTIGKINEDLEIRKFKTNALPLY
                     VITDHEGNPLNDPMPTNLNINEYKAWLDEGFRLFKAKQN"
     gene            complement(2870..4495)
                     /gene="lnt"
                     /locus_tag="PK288_10705"
     CDS             complement(2870..4495)
                     /gene="lnt"
                     /locus_tag="PK288_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPM19439.1"
                     /translation="MNKYGLSALSVTGGILSGLAWTSWCSGLILLTALVPFLIIEDHI
                     YENRSRFPGDAVFIYLLPGFLIFTLIAIGWIREIGIAVTAVVTICAAIMISVVWWGAH
                     KVKTIAGSFAGYLSLIAFWLLLDFLCLHTAILSPWLNLGNGLAKDITFIQWYEYTGTS
                     GGTLWVIISNLFLWFLFRSIKQKNRSAWILLLAWLLIIIVPFSASLLIFANLKSDKSD
                     GYEVVVVQPNFDPYHEKYTIPFDVQLERSIKLAEKAASDSTAWIIMPETVVDDPVNEE
                     MISENNYVSMLKKLNDKYPLAEIIAGMVTCVPDTPGKSQGCKAEQKQQDESCGTHFNS
                     ALNISSQRKIEIYHKSKLVPGFENIPVKGLFRFANRFMPVLGNANWSYGTQKNRAVFS
                     GSGKYPLTAPVICYESVFGEFVTGYVKLGAEALFIITNDGWWKDTKGYRQHLSYASLR
                     AIETRRPVVRCANTGVSCLINTLGQITEQTGWWESSVLKGIVFPQQRITSYVKYGDVI
                     LRSAAFISFFILLWTFLISRIRKKIKPPATWGC"
     gene            4858..>5345
                     /locus_tag="PK288_10710"
     CDS             4858..>5345
                     /locus_tag="PK288_10710"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPM19440.1"
                     /translation="MRKLAGLLFIILVCSSCGTTEERLSSKAVRIHRKILSIDTHCDT
                     PMEMMRTGYDLGLRNEKGCVDFPRMREGSLDGEFFAVFIPQGHRNDSMSNVVHLNALE
                     IFKRIHENVEKNSDMAGLAVTADDAFRLKKKGRLSAFIGLENGYPIGNDISRISQYYD
                     LGA"
BASE COUNT         1444 a         1132 c         1239 g         1530 t
ORIGIN      
        1 caaggttagc ataccgatat tcgagcatgt tggcgttaac aagcagataa aggcactgtt
       61 tggaaaaacg gtatcattta aacacggagc atatctgatc attgagcata ccgaggcatt
      121 tcatgttatt gacgttaaca gcggcaacag gtcccggtca ggtaatgacc aggaatcgaa
      181 tgccctggaa gtgaacatgg aggctgcacg ggagatagcc cggcagctca ggttaaggga
      241 tatgggcggt attattgtcg tcgattttat tgatatgcag tcatcagaga acaagcagct
      301 tctctttgac aggatgaagg aagtcatggc taacgaccgg accaagcata atatccttcc
      361 tctttccaaa ttcgggctaa tgcagataac acgccagagg gtccgccccg aaatgaatat
      421 tgtaaccgac gaaaagtgcc cgtcgtgcca gggaaccgga aaaagccggc cttcaattct
      481 gtttgttgat gagattgaaa gggctctttc ctttatcgtt gaacgcatca agacccggaa
      541 aatcattctg aatgtgcatc cattcgttgc agcctacctg cgttgcggat ttttccccat
      601 ttacaggaaa tggaacctga aatacaaaat actgataaag atccaggcag ctacctcata
      661 ccacgcactg gaatatcatt tttacgacag ggaaggcaat gaaatagacc ttgcctgaca
      721 ataaacctgt atttgaactt ttaacggggt ttatcgttga tcttgcagaa tttatgtttt
      781 agcaaaataa taatgcccga tatgaacaga aaactgcaat tccttttatt cctgcttgtg
      841 attttaagtc gcgggttgtc agctcagatt tatgatcctg tatcatggga gttcagttat
      901 gaaaaaaaag gaaataacag gtacgatttg attttcgcgg ccaccattga ggaaggctcc
      961 catatttatt ccatggatgt tcccgaaaac ggccccatac caacttcgtt catttttgac
     1021 accatgcccg gctataagct tgaggatagt gttttcgagg tcacaaaacc tgaagatctt
     1081 tttgatgagg cttttggttt caggataaag tccttcagta acagggctga gttcaggcag
     1141 actgtctatt ctgaagagcc tgcattcacg gtcagtggca cagtaaatta catgtcgtgc
     1201 aataaatccg cctgctcgcc gcctaaagat gttgaattca gtatccggat cggctcacct
     1261 gtatcttttc ccggaagtag tgctgctgcc gggacttctg gcaccggcag gcctgccgga
     1321 ccgggaagcg gattgctgaa atttttcctg atatcagtcc tggccggctt tgccggaata
     1381 ttgaccccat gcgtttttcc aatgatacca attactgttg catttttttc ccggggttct
     1441 gaaaacaggg tacagtcggt gatcaaggca ataatttttg gattgtccat tctgcttatt
     1501 tattcgctgc tggggattat cgtatcactg acaagcgcag gagcaggttt cgccaatacg
     1561 ctcagcaccc attggatacc caacctgata ttttttgtac tttttattct gttcgcaatt
     1621 tccttcttcg gtgcttttga gattgtcctg ccgaacagat gggtaactgg ggcagactca
     1681 aaggttgaca ggagaggatt actggcttca tttttcatgg gacttacaac cgttatcgtt
     1741 tcgttctcct gtaccggacc aataatcgga gtcctcctgg ttgaagctgc taccggtgac
     1801 tggcttcgtc ccactattgg gatgagtgga tttggacttg cgtttgcgct tcccttcacc
     1861 gtttttgcat tgttcccgtc aatgatgagc aggttgccta aatcgggcgg ctggcttaac
     1921 tcggtaaaag tatttctcgc ctttattatg ctcgctttca gcctgaaatt cctgatgacc
     1981 attgacagcg tttacaatct caggatactt accagggatg tgtatcttgc tatctggatc
     2041 gtgatcttct cactcctcgg gttttatctt cttggcaaaa ttaaatttcc ccacgatgcc
     2101 gataccccgc acctgggaat cttcagactc ttccttgtaa tagccgtatt ttcttttgtg
     2161 atatacctta tacccgggct tttcggagcg ccgcttaaag gcctttcatc cctccttcct
     2221 tctccgcaga cgtcggaatt cagacatctt ggaactgtgg gcaatattgc ggaacctgca
     2281 ggcaggcagg taccagtttt ttccaatggc ccttgttccg atgtaaagca tggggacata
     2341 tttgatatgc ctttaggcct gaacggatat ttcgacttcg agcagggatt gacttgtgca
     2401 agccagcaag gcaagcctgt attgcttgat ttcaaggggc atgcctgcgc aaactgcaag
     2461 aacatggaag ctaaggtatg gtcagatcct gaagttcttc agcggctgag agacaatttt
     2521 gtcatcgtgg cattatacgt tgatgacaga acccagcttc ctgaaaatga atggatcact
     2581 tccgggatag acggaaaggt aaagaaaacg ataggcaaga tcaatgagga ccttgagatc
     2641 agaaagttta aaacaaatgc actccccctg tatgtaataa ctgatcatga agggaacccg
     2701 cttaacgacc cgatgcccac caatctcaac ataaatgagt acaaagcctg gctcgatgaa
     2761 ggattcagat tgtttaaggc aaaacaaaac tgaactgaac atccggcttt aagaaattgt
     2821 tcctcttgtt gcctgaaagg aatgctttcc ggtcaatttt tggaccagct taacatcccc
     2881 aggtggcagg tggtttgatt ttcttccgta tccgggagat caagaaggtc caaagcagga
     2941 taaaaaagct tataaaagcg gctgatctta aaatcacatc accgtatttt acataagatg
     3001 taattctttg ttgcggaaag acaatcccct ttaatactga actttcccac catccggttt
     3061 gttctgttat ctgacccagg gtgttgataa ggcatgagac accggtattg gcacatctca
     3121 ccaccggtcg ccgtgtttca attgccctca atgatgcgta tgaaagatgc tgtctgtatc
     3181 cttttgtatc cttccaccaa ccgtcgttgg taattataaa taaagcttcc gcaccaagtt
     3241 taacataacc agtgacaaat tcgccaaaaa ccgattcata gcagatcact ggtgcggtca
     3301 gcggatattt gccagagccg gaaaagacag ccctgttctt ctgggtacca taactccagt
     3361 ttgcatttcc aagcacaggc ataaagcggt ttgcaaatct gaataaacct ttaacgggta
     3421 tgttttcaaa tccgggaaca agttttgatt tatgataaat ctcgatcttc ctttgagaac
     3481 tgatattcag ggcggaattg aaatgtgttc cgcaggattc gtcttgttgc ttctgttccg
     3541 ctttacagcc ttgacttttt cccggggtat caggaacgca ggtaaccatt ccggcaataa
     3601 tttccgccag gggatatttg tcattcagtt ttttcagcat gctgacataa ttgttctcac
     3661 tgatcatttc ttcattcact ggatcatcca caacagtttc tggcattatg atccaggcag
     3721 tggaatctga ggcggccttc tctgccagtt tgatggatcg ctccagttga acatcaaagg
     3781 gtatggtata tttttcatga tagggatcaa aatttggctg gacaaccacc acttcgtaac
     3841 catcggattt gtctgatttc agatttgcaa agatcagaag ggaagctgag aacgggacta
     3901 tgattatcag cagccatgcc agcaggagaa tccatgcact ccggtttttt tgcttaatgc
     3961 tcctgaacag gaaccagagg aaaaggtttg aaattatgac ccacagcgta cctccagagg
     4021 ttcccgtata ttcataccat tggataaaag ttatgtcttt tgccagtccg ttgccaagat
     4081 tgagccaggg tgaaaggatg gctgtgtgga gacacaggaa atcgagaagg agccagaatg
     4141 cgataagtga aagataccct gcaaaggaac ccgctattgt cttcacttta tgggcacccc
     4201 accatacgac ggatatcatt atggcagcac atattgttac gacagcagtt acagcaattc
     4261 cgatttccct gatccacccg attgcaataa gcgtgaatat taaaaagccg ggtaaaagat
     4321 agatgaatac agcatccccc ggaaatctgc tgcggttctc gtagatatgg tcctcgatta
     4381 taaggaaggg gacaagggca gtcagaagta tcaatcccga acaccagctg gtccatgcga
     4441 gtcctgagag tattcctcct gtaactgata gtgcggaaag gccgtattta ttcatttaat
     4501 gtttactgct gttcggggtt tattgtataa aagtagtcac tccttttgcc atagccaagg
     4561 ttaaaaacgg aatggtttag taaggtcaaa aaaaaagtta taaatttgaa tgttatttga
     4621 accgggagca ggtaagctgg tcaggcagtt gcttaaggaa tactgcagaa agtttaataa
     4681 taaactccgg aaaatgcttt tactgtccaa aaaagtaatt ttccattctt ttcatggttg
     4741 cccctggctt aaaagtcggg ggcttttgta tgttaaatag tgttaattga aggaatgaat
     4801 ttattttgcg gaagttcaat ttgtttatct ttcaaaacta aaaggggcaa tatgtgtatg
     4861 agaaaactgg caggtctgtt gtttattata cttgtttgtt cttcctgcgg caccacggag
     4921 gaaaggctgt cgtcaaaggc agttaggatc cacaggaaga ttctgtcgat agatacccat
     4981 tgtgataccc cgatggagat gatgcgtacg ggttatgacc tgggattgcg caatgaaaag
     5041 ggttgtgtcg attttccaag gatgagggaa gggtcgcttg atggagaatt ttttgccgtt
     5101 ttcatccctc agggtcacag aaatgattca atgagcaatg ttgtacatct gaatgcgctg
     5161 gagattttta aacggattca tgagaatgtg gagaagaatt cagacatggc agggttggca
     5221 gttacggctg atgatgcttt cagattaaag aaaaaaggca ggttatcggc tttcataggg
     5281 ctggaaaatg gttatcccat aggcaatgac atatcccgta ttagtcagta ttatgatctt
     5341 ggagc
//
LOCUS       DAOOYG010000163         5307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_18030_length_5307_cov_5.211538, whole genome shotgun sequence.
ACCESSION   DAOOYG010000163 DAOOYG010000000
VERSION     DAOOYG010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18030_length_5307_cov_5.211538"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PK288_10715"
     CDS             <1..1150
                     /locus_tag="PK288_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPM19441.1"
                     /translation="FEKPVLVAITRVAALGIILNSLCSVQVAILTRRMDFRKLTWIHL
                     LSALVSGITGLVLALRGYEVWALVFQTLSGNVIYLAGLWLSTGWRPAFMFDFRRFRAL
                     FDFGYKVLLQGLTDVIFTKSYYPLIGKLFPVAQLGFYTNASRFHEIFIRQTSYSANRV
                     VFPAFAAIQDNRERFNGNYLRSFNLMVFLMFLGSLVLIISSRPFISVALTAKWLPSVP
                     FMQLLFIEGFFLPLLIFNTNILYTIGKGGISLKIDIFRKAMILVSIFLLFRLGIEALI
                     TGQVFSTTVSMIFSALAVGRIRGIELRSLFMPLLKLLLITGLCFALSYLIEIVTGGSD
                     WPELIMKVTIVPAVFVLLAWLAGINGIRELRAFVGEFRPGAAQADELL"
     gene            1175..2599
                     /locus_tag="PK288_10720"
     CDS             1175..2599
                     /locus_tag="PK288_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19442.1"
                     /translation="MAKGVIKWFIGWPDLVKYLGLISVTLGVTYYAPASIRFIWYVIM
                     LVSYSFSKNEPLWLALFLSTSDGFIGFFGLYEATIPLLPGLPAVEVAQFYILLTVVKA
                     AGRNLKPVLFYHRYFQILIIYLIFSVIWGQMMGLSGGLNVYFRVIKGIIPLLLFYSVP
                     RLFTDRGMYERFFRIVFVIVLLAFAAQLFTLFTGMTPMEAGGLTADEKYEKDEFRVFY
                     NASSTLLGLFGALFVLTEKRSSPLSRLIPFAVLIAAISMALLSATRGWIISFSLVIML
                     SVLLTGMIRSKRNISIVLISIPVIIWALANPTISKQVDYARERLETMGAISEGDLTAR
                     GTLQRLDFRSQKVMDGWRESPLFGWGLSDKGYEYLDDHVGNQSVLALYGVIGFVLLNG
                     FLIYFAYMLMGLYLRFPPGHQYRDEVIVFVVFLVGWFFIHSTSGQQFSFSGMPEKIIP
                     QALFFSFGALQFNKSISTLNGKKV"
     gene            2583..3647
                     /locus_tag="PK288_10725"
     CDS             2583..3647
                     /locus_tag="PK288_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM19443.1"
                     /translation="MEKKFERALLLCPAQYSLSNSLRSILGHMAGEVRYSDIRSAISS
                     RIMKLNSQAFRLPYGVRKRWDRYFLEKVNEMILTQVEEFRPDLVMVYNSLYMVPETCR
                     TVSSRSKLVFFMGDSPFYTPQNNYYLSCLPHADLILSPDSHWQQQMVTMGLEKTLYFV
                     PGVDTSSYYRLTPENTQTGYGRSGVLYVGACYLNSWGYKKAMLMNYFTRFDFRLYGNS
                     AWRRWFSFFPDLEKVFTDSGFIAQEEMNRMYNRAAVIPVDGNPGIINGVHLRMFEALA
                     AGALPLIEYRNDVDSLLFNGFEDQLPLVRDYRKVADMAEYYLNNERERSGLVYRMDEF
                     LAREYSHELNASRLAEALGR"
     gene            3647..4756
                     /locus_tag="PK288_10730"
     CDS             3647..4756
                     /locus_tag="PK288_10730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPM19444.1"
                     /translation="MSITDYRVCLVHYTSAPGGIELLMPEIIRVFPENTFTIFVIRPA
                     AIGEFDVYEGVPVKVFRGSHNNLTAAFKLWCYARKHKQTVFHGFNTGPFFMMVLRLAG
                     VKKPVYSIRGTIHYCGTLQKILRKIVWRASLGNRYRIIANSEYSRDIFLKFLAPFEPH
                     IEVLYNPVCSSRIKPASEKVVRDRLNIIYTGRLADGKNLFRWIEMAVAIHSYRSDAMF
                     YLFGDGPLRKELEKYCRTLRAEGYISFMGFQSDLSEAYSRADLMMFLSEAESFGNAVV
                     ESILSGVPVIALAIPSVKEIFRDFPQFLISTGPGMDEEIIDKIKAINELKAAVPVACR
                     QFRKRFSLEQHVKGLCSLYESLGLSVNDTLSTKKR"
     gene            4753..>5307
                     /locus_tag="PK288_10735"
     CDS             4753..>5307
                     /locus_tag="PK288_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19445.1"
                     /translation="MKISFLAKITGNMPEKQTGIMFADPPELTIHLTGDNFEFMAWGD
                     PIFDDRFLADLPVRLSTEFVTGNLSGHYYFCINDITRKEIIIGTSPFSIVPLYYCVKD
                     SDIIVSDNVFTIAGHTGLKNVSARFITESLLFNYPLYNCSLVEGISLLESGSALIAAR
                     SGIRTVRHTAPEEWFADCPVTWRKS"
BASE COUNT         1306 a         1110 c         1301 g         1590 t
ORIGIN      
        1 cttcgaaaag cctgtactgg tggcaattac acgtgtggct gccctgggaa taattctcaa
       61 ctctctttgt tctgtccagg tggccattct tacccggagg atggatttca gaaagcttac
      121 ctggatacac ctgctgagtg cgctggtatc gggtattacc ggattggtcc tggcattgag
      181 gggttatgag gtctgggctc tggtatttca gaccctgtca ggaaatgtaa tatacctggc
      241 aggcctttgg ctatcaacag gctggaggcc cgcattcatg tttgatttca ggaggttcag
      301 ggctctgttc gatttcgggt ataaagtgtt gctgcaggga ctgacagacg tgatattcac
      361 gaagagttac tatccgctga ttggtaaact gttccctgtc gcccagctgg gtttctatac
      421 caatgccagc cggtttcacg agatattcat ccgtcagacc tcttactcag caaacagggt
      481 ggtattccct gcttttgcag ccattcagga taaccgggag agattcaacg gcaattacct
      541 gaggtcgttt aacctcatgg tattcctgat gtttctggga tcactggtac tcataatctc
      601 gagccgtcct tttatttcag ttgcgctgac agcgaaatgg ctcccttcag tacctttcat
      661 gcaactttta tttattgagg gtttttttct tccgctgttg atattcaaca ccaatattct
      721 gtataccata ggtaaaggcg ggatttcact gaagattgat atcttcagga aagcaatgat
      781 ccttgtcagc attttcctgt tgttcagact gggtattgag gctcttatta ccggccaggt
      841 gttttcaaca acggtttcta tgatattttc ggccctggcg gtgggcagga tccggggcat
      901 tgaactgcgg tcactgttca tgcctttgct caaactgctc ttaattactg gtttatgctt
      961 tgccctgagc tatctcattg aaatagttac aggcgggtca gactggccgg aactcataat
     1021 gaaagtcacc attgtccccg cagtttttgt attactggca tggcttgcag gcattaacgg
     1081 gatccgtgaa ctcagggctt ttgttggtga attcaggcct ggagcggcac aggcagacga
     1141 attattgtag gttggtttta actgcagcct gatagtggca aaaggagtaa taaaatggtt
     1201 tatcggctgg ccggatcttg tcaaatatct cgggcttata tccgtgaccc tgggagtaac
     1261 ctattatgct cctgcttcta tcagatttat ctggtatgtg ataatgctgg tatcctactc
     1321 attttcaaaa aatgaacccc tttggcttgc tcttttcctg tcaacctcag acggctttat
     1381 cggcttcttc ggcctgtatg aggcaactat tcccctgctg cccggattac cggcagttga
     1441 ggtcgcacag ttttatattc tgctgaccgt tgtcaaggct gccggcagga atctaaagcc
     1501 tgttctgttt tatcacaggt atttccagat attgattata tacctgatat tcagtgttat
     1561 atggggacaa atgatgggat tgtccggggg gttgaatgtt tatttccggg ttattaaggg
     1621 cattatcccg ctgttgttgt tctattcagt tcccaggctt ttcacagacc ggggtatgta
     1681 cgaacggttt ttcaggatag tgtttgtgat cgttctcctt gcattcgcgg cccagttgtt
     1741 cactttgttt acagggatga cgccaatgga ggccggtggc ttaacggcgg atgaaaaata
     1801 tgagaaagac gaattcaggg ttttctacaa tgcaagtagt acattgcttg gcctgtttgg
     1861 ggcattgttc gttcttactg aaaagagaag cagtccacta agcaggctga ttccttttgc
     1921 cgtattgatt gccgcaattt ctatggcttt gctctctgct acaagaggct ggatcattag
     1981 cttcagcctg gtaattatgc tttctgtgct tcttaccgga atgatccgtt caaagaggaa
     2041 catcagtatt gttttaatat ctatccctgt cattatctgg gcacttgcca atccgaccat
     2101 cagcaagcag gttgactatg cccgtgaacg gcttgaaacc atgggggcta tttcagaagg
     2161 agatctgaca gcccgcggaa cattgcaacg acttgatttt aggagccaga aggtaatgga
     2221 tggctggagg gagagtccct tgttcgggtg gggattgtca gacaaaggat atgaatatct
     2281 tgatgatcat gttggaaatc aaagtgtact ggctctgtat ggtgtgattg gcttcgtgct
     2341 tcttaacggt tttctgatct attttgccta tatgttgatg ggattgtact taaggtttcc
     2401 tccggggcat caatacaggg atgaagtgat cgtatttgtg gttttcctgg tgggatggtt
     2461 tttcatccac tcaaccagtg ggcagcagtt cagcttctca ggtatgccgg agaaaattat
     2521 tcctcaggct ctctttttca gtttcggagc gcttcagttt aataaatcaa taagcacatt
     2581 gaatggaaaa aaagtttgaa agagcccttc tgctttgccc tgcgcaatat tcgctgagca
     2641 attcccttcg aagtatactg ggtcatatgg ccggagaggt aagatactcg gatatcagat
     2701 ctgcaataag cagccggata atgaaactca acagccaggc tttccgatta ccttatggag
     2761 tcagaaaaag gtgggacagg tattttctgg aaaaggtcaa tgaaatgatt ctaacacagg
     2821 ttgaagaatt caggccagac ctggtgatgg tatacaattc cctttacatg gttccggaaa
     2881 catgccggac tgtcagcagc aggtcaaagc ttgttttttt catgggtgac tctcccttct
     2941 atactcctca gaacaattac tatctctcct gtctgcctca tgctgacctg atcctttctc
     3001 cagattccca ctggcagcag cagatggtaa caatgggtct ggaaaagacc ctctattttg
     3061 ttccgggtgt ggatacttca tcttattaca gattaactcc tgagaatacg cagacaggct
     3121 atgggaggtc aggtgtactt tatgtcggtg cctgttacct caactcgtgg ggatataaga
     3181 aagccatgct catgaactat tttaccaggt tcgatttccg cctgtatgga aacagcgcgt
     3241 ggaggaggtg gttttcattc ttcccggatc tggagaaagt ctttaccgat agcggcttta
     3301 tcgcccagga agagatgaac aggatgtaca acagagcggc tgtgatcccg gttgatggta
     3361 atcccggcat aataaacggt gttcaccttc gaatgttcga ggcgctggca gccggtgcac
     3421 tgcccctgat tgagtacagg aatgatgtag acagtcttct tttcaatgga tttgaagatc
     3481 aactgcccct ggtgcgtgat tacagaaagg tggcagatat ggctgagtat tacttgaata
     3541 acgaaaggga acgttccggg cttgtttaca ggatggacga attccttgca agggaatata
     3601 gccatgaact gaatgcttca agattggctg aagcactggg ccggtgatga gtattactga
     3661 ttacagagtc tgcctggttc attatacctc cgctcctgga gggatagagc tacttatgcc
     3721 ggagataatc agggtgtttc ccgaaaacac ttttacaata tttgtgatca ggcctgccgc
     3781 cataggagaa ttcgatgtat atgaaggcgt tccagttaag gtattcaggg gttctcataa
     3841 taacctgacc gctgccttta aactgtggtg ttacgcacgc aaacacaaac aaactgtttt
     3901 tcacggattt aatacaggac ccttttttat gatggtactc cggttggctg gtgtgaagaa
     3961 gccggtctat tccatcaggg gaaccattca ctattgtgga actttgcaga aaatcttgag
     4021 aaaaattgta tggagagcct ctcttggaaa cagatacaga ataattgcca attctgaata
     4081 tagccgtgat atattcctga aattcctggc tccgtttgaa cctcacattg aggttcttta
     4141 caatcctgtc tgttcatcac gaataaaacc ggcatctgaa aaggtagtca gggatagact
     4201 gaacatcatt tatacaggga ggcttgctga tggtaagaac cttttccggt ggatcgaaat
     4261 ggcagttgca attcacagtt acaggagtga tgcaatgttt tatctttttg gtgacggtcc
     4321 ccttcgaaag gaattggaaa agtattgcag aactttgagg gcagaaggtt atatcagttt
     4381 tatgggcttt cagtccgatc tttcagaagc atacagccgg gctgatctga tgatgttcct
     4441 ttctgaagca gagtcatttg gaaatgccgt agtagagagt atactgtccg gagttcctgt
     4501 aattgccctg gccattcctt cggtaaagga aatattccgg gatttccctc aattcctgat
     4561 ctctaccggg cccgggatgg atgaggaaat catagataaa ataaaagcca ttaatgaact
     4621 gaaagcagct gttcccgttg cctgtcgcca attcaggaaa agattttcac tcgaacagca
     4681 tgtgaagggc ttgtgctctc tttacgaaag tcttggtctt tcagttaatg ataccctgtc
     4741 aactaagaaa agatgaaaat ctcatttctg gcaaagatta ccgggaacat gccggaaaaa
     4801 caaacaggga ttatgtttgc tgacccccct gagctcacta tccatcttac aggagacaat
     4861 tttgaattca tggcatgggg tgatcctatt ttcgatgacc ggttcctggc agatcttcct
     4921 gtcagactga gcacagagtt cgttaccgga aacttgtcag gtcattatta tttctgtatc
     4981 aatgacataa caaggaagga gataataatt ggcacctctc ccttcagtat tgtcccgctc
     5041 tattactgtg tgaaggattc tgatatcatc gtgtcagaca atgtctttac aattgccggg
     5101 cataccggat tgaaaaatgt gtcagcacgg tttattactg agtcgcttct ttttaattat
     5161 cccctttata actgcagtct ggttgaaggc atatcactgc ttgaatcagg ctctgctctg
     5221 attgctgcga gaagtggaat aaggacggtg agacacacag ccccggaaga gtggttcgcc
     5281 gattgcccgg ttacctggcg aaagagt
//
LOCUS       DAOOYG010000164         5222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_18429_length_5222_cov_7.138378, whole genome shotgun sequence.
ACCESSION   DAOOYG010000164 DAOOYG010000000
VERSION     DAOOYG010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18429_length_5222_cov_7.138378"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PK288_10740"
     CDS             <1..640
                     /locus_tag="PK288_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19446.1"
                     /translation="VAEITTASLIAGMKVQLVFGNGPGFYSSNDTIPGRLVESVITRL
                     ENEIIPLKNKAESVIQSLDTTLGSINDIMTPEFRNNVNAGIASLRNTADGIEKADLGA
                     SLENIKKLTEMLAENTTRLSRTFENLESVTDTLVAADLYSTVNNLRSSLGKLTVLVES
                     LTDGKGTAGQLMTNDSLYINLNRSLASLDLLLQDLKSNPRRYVHFSMFGRKN"
     gene            988..2418
                     /gene="dnaA"
                     /locus_tag="PK288_10745"
     CDS             988..2418
                     /gene="dnaA"
                     /locus_tag="PK288_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPM19447.1"
                     /translation="MNKSHIDVWNNCLQIIKDIIPSVSFKTWFDPIIPLKLENNVLTI
                     QVPSPFFYEYLEEQYIDILRKTLRREIGSDAKLEYNVVLENANYSDGKPYTVKYPSRN
                     KTDLKNKPVQVPFDVTQPSIRNPFVIPGIKKVHFDPRLNPDYNFNNFVEGECNRLARS
                     AGIAVGNNPGGTAFNPLMIYGDSGLGKTHLAQAIGILVKEKHPDKVVLYVNANKFQTQ
                     FVESVRNNNKNDFLHFYQMIDVLILDDVHEFAGKEKTQDMFFHIFNHIHQSGKQLILT
                     CDRPPVELQGMEQRLLSRFKWGLQADLQRPDYDTRLAILKKKAYNDGIELPEEIFEYL
                     AENITNNIRELEAVLISLYAQSTLNRKEITLDLARQMVEKLVSTSRREITIEYIQKIV
                     CDYYKIPIELIQGKTRKREIVQARQVSMYFSKNLTKASLASIGSHIGGKDHATVLHAC
                     KTVNNLIDTDKHFRNQINEIEKKLKV"
     gene            2421..3587
                     /locus_tag="PK288_10750"
     CDS             2421..3587
                     /locus_tag="PK288_10750"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPM19448.1"
                     /translation="MDILSFTEKFFEKYGNRNEKPVLFFSPGRVNLIGEHTDYNGGFV
                     FPCALNFGTYLAIRTVKDKTLRFSTLNFDDDDEVQLANNLSKRGNKWSNYPLGVISEF
                     LKRKISVGGLEMLFYGDVPNGAGLSSSASIEMVTATALNDIYSAGLKTLDLVILSQKA
                     ENEFVGMNCGIMDQFAVGFGKKDHAIFLNCDTLDYENVPVNLGNNALIITNTNKRRGL
                     TDSKYNERRSECEKAVELLQSFRPIKNLSELNTDDTGFLEEFIKDPVILKRARHVISE
                     NGRVLEAVKVLKENNIRRFGELMNQSHDSLKDDYEVTGNELDTLVYEGRKLPGVTGTR
                     MTGAGFGGCTVSLVEKEHVPGFISGLKSVYQEITGLVPDFYQPEISDGAGRISW"
     gene            complement(3672..>5222)
                     /locus_tag="PK288_10755"
     CDS             complement(3672..>5222)
                     /locus_tag="PK288_10755"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPM19449.1"
                     /translation="AIFLSPELNLNKFIKPDFSIFRVCDVGEDILHREIAIAFFNGYG
                     TELNMYRPGGRDENYNDDLDFLAKTTFILRQNNDAFLDKNWIPLIGSVEDNVYINQWH
                     SGRKKIFTVLNMNSNGVRDRILWIDADSGLHYVSLWDHENLTPELVDGKLCLPVNADG
                     WQASYNGTRREGSVDCIAAFPVILNSSLEGDRIRISSNSPGKLVVWKGSPAYGGESRE
                     FSLKSDTTLSVKDMFGYYEGKIVIQLLSEKILMDENIIRIKGGQPWLISKVTRTPRMT
                     EYDRNMVLVPGTDFAYSVSAYDNFIPYPATGNYTHKIDSFLIDRFPVTNEQYYEFISA
                     SGYRPADTTRYLRHWEYGIYKPGTEKYPVVYVGYDDIRAYAKWAGKRLPTEAEWQLAA
                     QGQDKRLWPWGNEFHATFCNNAFGKSTPVDAFSKGASPYGVMDLVGNVWQVTNDMYFN
                     GSYYFLVIRGGSYFRPDSSRWYIQGGPQPLDRTQIMLLISPGFDRSATVGFRCVKDID
                     PDNYKGKK"
BASE COUNT         1574 a         1164 c         1105 g         1379 t
ORIGIN      
        1 tgttgccgag ataaccactg catccctaat cgcaggaatg aaagtacagc tggttttcgg
       61 aaacggaccc ggtttctact cgagtaatga caccataccc ggccggcttg tcgaatctgt
      121 aatcaccaga ttggaaaatg aaatcattcc gctgaaaaac aaggcagaat ctgttattca
      181 aagcttggat acaaccctgg gatccataaa tgatataatg accccggaat tcagaaataa
      241 cgtcaatgcc ggaatcgcca gcctgagaaa tacggccgat ggaattgaga aagctgatct
      301 gggagcctca ctcgaaaata tcaaaaagct gacggaaatg ctggccgaga atacaacaag
      361 acttagcagg acatttgaaa accttgaatc tgtgaccgat accctcgtgg cagctgacct
      421 gtattcaaca gtgaacaacc tcaggagcag ccttggaaaa ctgactgtac tggtggaaag
      481 tctcaccgat ggaaaaggta cggccggaca actgatgacc aatgattccc tgtatataaa
      541 tctcaacagg agtcttgcaa gccttgacct ccttttgcag gatttaaaat caaatccccg
      601 caggtatgtt catttctcaa tgtttggcag gaaaaactga cctgaaacat ttttatttct
      661 tataaaaaag ggttatcttg atctaaaaat ttcctgactg accgattgaa aaatcacccc
      721 ggctttgcat attaaaattg cataaaaacc gatcagaaca cctaaggaat tgtgacacat
      781 gggcaaacaa aacaacctga tcgtaacata ttgatataca gatatatata ataagcagaa
      841 atattttttt ctttaagtta ctgatggtta aaaccatata ctttttttga atttatcaag
      901 tgaaaataaa tttttttttg acattttttt tgtaaatctt gcctcctgat ttttcctatt
      961 ttggaacctt tcaacgaaaa tgcctccatg aataaatcgc acattgatgt ttggaataat
     1021 tgtctgcaga taataaagga tattattcca tccgtcagtt ttaaaacatg gtttgatcca
     1081 ataattcccc tgaaacttga aaacaatgtc cttaccattc aggttccaag tccttttttc
     1141 tatgaatatc ttgaagaaca gtatatcgac atcctgagaa aaaccctgag acgcgagatt
     1201 gggtccgatg caaaactcga gtacaacgta gtgcttgaga atgcaaatta ttccgacgga
     1261 aagccgtata cggtgaaata tccgtcgaga aacaaaaccg acctcaagaa caagcctgta
     1321 caggttccct ttgatgtcac ccagccttca atcagaaatc cgtttgtgat accgggcatc
     1381 aaaaaggtcc atttcgaccc caggctcaat ccggattaca atttcaataa tttcgttgag
     1441 ggtgaatgta acaggcttgc cagatcggca gggatcgcag taggcaataa tcctggcggc
     1501 actgctttca atccgctgat gatctacggt gattcagggc ttggcaaaac gcaccttgca
     1561 caggcaatcg gtattctggt gaaggaaaaa catcccgaca aggttgtgct ttatgtaaat
     1621 gccaacaagt tccaaaccca gtttgtagaa tcagtcagga acaacaataa aaatgacttc
     1681 cttcattttt accagatgat tgacgtactg atactggatg acgttcatga atttgccggg
     1741 aaggaaaaaa cccaggatat gttctttcac atattcaatc atatccacca gtcgggaaag
     1801 cagctgatcc ttacctgtga ccgtccgccc gttgaactcc agggaatgga acaaagactg
     1861 ttgtccaggt tcaaatgggg gcttcaggct gatcttcagc gcccggatta tgatacaagg
     1921 ctggcaattc tcaaaaagaa ggcatataat gacggaattg aactgccaga ggagattttc
     1981 gaataccttg ccgaaaatat caccaacaat ataagggaac ttgaagcagt attgatatca
     2041 ctgtatgcac aatcaaccct gaacaggaag gagataaccc ttgatcttgc acggcagatg
     2101 gttgagaagc ttgtaagtac ttcacgccgt gaaattacaa tcgaatacat ccagaaaata
     2161 gtttgcgact attataagat cccaatagaa ctgatccagg gcaaaaccag gaaaagagag
     2221 atcgtacagg ccagacaggt atcaatgtat ttctcaaaaa accttacaaa agcatcgctt
     2281 gccagcattg gttcacatat cggagggaag gatcatgcaa ccgtcctgca cgcatgcaaa
     2341 accgtcaata atctaattga caccgataaa catttcagaa accagataaa tgagattgaa
     2401 aagaaactaa aagtataatt atggacatat tgtcttttac tgagaagttt tttgaaaaat
     2461 acgggaacag gaatgaaaag cctgttcttt ttttctctcc cggaagggta aaccttatcg
     2521 gtgaacatac cgactataac ggaggcttcg tttttccctg cgccctcaat ttcggaacat
     2581 accttgcaat acgtactgta aaggataaaa cactcagatt cagcacactc aacttcgatg
     2641 atgacgatga ggttcagttg gccaacaatc tctcaaagcg tggaaataaa tggagtaact
     2701 atccgctcgg ggtgatcagt gagtttttga aaagaaagat cagcgtaggt ggcctggaaa
     2761 tgctttttta cggcgatgtt cccaacggag ctggtttgtc ctcatcagcc tcgatcgaaa
     2821 tggtcacagc aactgcactc aacgacattt acagtgcggg actgaaaaca ctcgacctgg
     2881 tgatcctgag ccagaaggcc gaaaatgaat ttgtcggaat gaattgcgga atcatggatc
     2941 agtttgccgt gggtttcggt aaaaaggatc atgcaatatt cctcaactgt gataccctcg
     3001 actatgaaaa tgtacctgtc aatctgggta acaatgcgct tataataaca aataccaata
     3061 agagacgcgg gctaaccgat tcaaaataca atgaaagaag gtctgaatgc gaaaaggcag
     3121 ttgaattgct tcagtccttc cgtccgatta aaaacctgag tgaactgaac actgatgaca
     3181 caggttttct tgaagaattc atcaaagatc ctgtcattct gaaaagagcc aggcatgtca
     3241 taagcgaaaa cggcagggtg ctggaagcag tgaaggttct aaaagagaat aacatacggc
     3301 gattcggaga actgatgaac cagtcgcacg attcgcttaa ggatgattac gaggtgacgg
     3361 gcaatgaact ggatactctt gtttatgaag gcagaaagct gccgggtgtt acaggaaccc
     3421 gcatgaccgg cgcaggtttt ggaggatgca ccgtaagcct ggtggaaaag gaacatgttc
     3481 ccggattcat ttccggattg aaatcagtat accaggagat cacaggtctc gtccccgatt
     3541 tttatcagcc tgaaatttcg gatggtgcag gaagaatatc ctggtaaaca acctgaaacc
     3601 gttcagtccc aggctgatat ctgtaattca gtccgatgat tggtatttag cggatttttc
     3661 tgtctggcaa actacttttt tcccttataa ttatccggat caatatcctt cacgcatctg
     3721 aagcctacgg ttgcgcttct gtcaaaccca ggtgatatca gcagcatgat ctgggtccgg
     3781 tcgaggggct gggggcctcc ctggatatac catctgctgg aatcgggacg gaaataactt
     3841 cctcccctga ttacgagaaa ataataggac ccgttgaagt acatatcatt tgtaacctgc
     3901 catacatttc ctacaagatc cattacccca tacgggctgg ctccctttga aaaagcgtcg
     3961 accggagttg attttccgaa tgcattatta cagaaagtcg catgaaactc attaccccat
     4021 ggccagagcc ttttgtcctg accctgggca gcaagctgcc attcagcctc cgtaggcagt
     4081 cttttccctg cccattttgc atatgccctg atatcgtcat aacccacgta tacaaccgga
     4141 tatttttccg tgccgggctt ataaatgcca tattcccaat gtctcaggta ccttgtggta
     4201 tcagcaggcc tgtatcccga ggcagaaata aactcgtaat actgttcatt ggttacagga
     4261 aatctgtcga taagaaaact gtcgatctta tgggtataat tgccggttgc aggataagga
     4321 atgaaattat catatgctga tactgagtaa gcgaaatcgg ttccgggcac aagcaccatg
     4381 ttccggtcgt attctgtcat cctgggtgtc cttgtaacct tgctgataag ccagggctgc
     4441 ccgcctttga tccttataat attttcatcc atgaggatct tttcgctgag aagctggata
     4501 acgatcttac cttcataata tccaaacata tcctttacac tcagtgtggt atcgctcttc
     4561 aggctgaatt ccctcgattc tcctccatac gcgggagaac ccttccaaac taccagcttc
     4621 ccgggtgaat tgctgctaat ccttatccga tcaccttcaa ggctggaatt cagtataacg
     4681 ggaaatgccg caatacaatc aaccgagccc tctctcctgg ttccgttgta tgatgcctgc
     4741 catccgtcag cattcaccgg caggcacaac ttgccgtcaa ccagttcagg ggtaagattt
     4801 tcatgatccc acaatgaaac ataatgcagg ccgctgtcag catcgatcca caggatcctg
     4861 tctctaactc cattactgtt catattcaag accgtaaaaa tcttcttccg gccggaatgc
     4921 cactggttta tgtaaacgtt gtcttctact gatccaataa gagggatcca gttcttatcc
     4981 aggaaggcat cattattctg cctcaggata aatgtcgttt tggcaaggaa atcaagatca
     5041 tcattgtaat tttcgtccct gcctccgggc ctgtacatgt taagttcagt tccgtagccg
     5101 ttaaagaaag ctatggcaat ttccctgtgg aggatgtctt cccccacatc acacaccctg
     5161 aaaattgaaa aatcgggttt aatgaactta ttaagattta gttcagggct gagaaagatt
     5221 gc
//
LOCUS       DAOOYG010000165         5135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_18842_length_5135_cov_7.596654, whole genome shotgun sequence.
ACCESSION   DAOOYG010000165 DAOOYG010000000
VERSION     DAOOYG010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18842_length_5135_cov_7.596654"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1358
                     /locus_tag="PK288_10760"
     CDS             186..1358
                     /locus_tag="PK288_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPM19450.1"
                     /translation="MDLLRSNPMYRYLMILGICANAGLQGWTSLFTNYAKEIVGVNGF
                     QLGVSQAVREIPGFLSVAAIFLLFFFKEHRLSSWAVILLGIGTAFTGIFTSFTGLLAT
                     TFTMSVGFHFFETTNKSLTVQYFNTTESPVVFARMRGYTALANIAVGFAIWLMSFMLP
                     YMQMFLLLGLIISLTGISMLFSNPAGKEMPHQHKKLIIKKKYWLFYMLNFLSGTRRQI
                     FTVFAIFLLVEQYQLSLRVVTGIYVINYALTYLLNPQISRALNKYGERIVLSLESASL
                     IVLFLGYAFINNVWVIIGLFVMDSIFFNFSIGLNTYLQKTADPEDLGPSTAVGFTINH
                     ISAVIIPVFGGALWMLNWRLPFLVGTGFAILSLYFTQLITTDYHEKRALSFIARLR"
     gene            1503..3530
                     /locus_tag="PK288_10765"
     CDS             1503..3530
                     /locus_tag="PK288_10765"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPM19451.1"
                     /translation="MNRVFLIAFLSAFFYFQLSAQDFTDSNLPIVIINTDHGMEIPDD
                     PRILADMKIIYRGPGLRNYVSDQNTPGYLNYNGRINIEIRGSSTQVAEKKQYGLTTLK
                     NDDKSNNNVKLLGLPKENDWILNGMVFDTALIRDYLSYNLSRQIGEYASRTVYCEVII
                     NGNYRGLYVLQEKIKADENRVNIIKIGVNDNSYPEVTGGYITKSDKTTGGDPVAWTLP
                     SWFDTPVDFIHELPKPKNVTSQQNDYIRSRFRLLQETSIAGDTSPLTGFPSIIDITSF
                     IDFILLNELASNADGYMYSTFFHKDRNGKLRAGPIWDFDLTYGNDLFFWGYDRSKTNV
                     WQLSNGENDGARFWKELFENPLFRCHAAKRWNSLTRPGQPFNQEVIESFIDRTVETIS
                     EAVGREYARWGQKADHSRLIADLKLFISRRIAWISANIGSSTACENTPVPPLVISKID
                     YHPESSSEFPDSKDLEFIEIKNNGNSRVDLTGIYFGGTGLVFQFQPYSSLGPLESIII
                     AGNSAAFRLKYGFTPFATFSGSLPDEGKELVLADAFGNIIDLVSYSNKAPWPDADGNG
                     SYLKLTDPDLDNNDPSNWIATRETLVNENIISADPATVLFPNPVANILNVRNNTLIIS
                     LMLYDVLGRPVKISEVNSLECQLDVTGLPSGTYFLKIVTLSGTLTERLLKH"
     gene            complement(3552..4439)
                     /locus_tag="PK288_10770"
     CDS             complement(3552..4439)
                     /locus_tag="PK288_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HPM19452.1"
                     /translation="MKKITITFTSLLFAAFLWGQQIPHPSLPYKMEELSSPRFVQALE
                     LSKGVCIIPLGIIEKHGPHLPLGTDLFEARQAATEAAKKEYAVVFPPYFAGQIFEAKH
                     QPGTIAYSSELMWKMLEETCEELARNGLKKIILVNGHGGSTSFLEYFCQSQLASRKDY
                     VVVLFRQEDDPVHSKEIEKLRKAKLDGHAGEEETSMMYSIRPDLVDVGAITKESGLDQ
                     KRLEKMPFGYTGIWWYAKFPNHFAGDVVVPDKELGELIFKIWSDQIVELIRYLKNNNS
                     IEQLQEEFYRRAEDPLKKK"
     gene            complement(4482..4772)
                     /locus_tag="PK288_10775"
     CDS             complement(4482..4772)
                     /locus_tag="PK288_10775"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPM19453.1"
                     /translation="MQFLLVAYDGNDPDALERRMRIRQEHLGKIAILKKTGEMLFGGA
                     ILDDDGRMIGSMVVYEFPDREALEKRLKEEPYITGGVWQKIEIHPFRLAAIE"
     gene            4952..>5135
                     /locus_tag="PK288_10780"
     CDS             4952..>5135
                     /locus_tag="PK288_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HPM19454.1"
                     /translation="MTKHFRFLKERKKKVHKPVFGALDILRYIGPGMLVTVGFIDPGN
                     WAANIASGSEFGYTLLW"
BASE COUNT         1423 a         1191 c         1073 g         1448 t
ORIGIN      
        1 ttaactatct tattgacatt caaacaatta tattaatatt taacgaagta ttgtttatta
       61 aagatgcttt taaatgaatc aaacggctat ttatcaaaat tgaaaaatga gaattaataa
      121 tccgtaaaaa aatttcagtc agaaattaat aataatatta cttttaccgg ctcatttaaa
      181 ttccgatgga ccttctccga tcgaatccga tgtacaggta tcttatgata ctggggattt
      241 gcgccaatgc gggcctccag ggatggacct cccttttcac gaattacgcc aaggagatcg
      301 tgggtgttaa cggattccag ttaggagtat cgcaggcagt aagggaaatt ccgggctttc
      361 tgtctgttgc agccattttc ctgctgttct tcttcaagga acacaggctt tcatcatggg
      421 cagtgatact gctggggata gggacggctt tcacaggtat tttcacatcc ttcaccggat
      481 tactggccac aacctttaca atgtcggtcg gatttcattt tttcgaaacc accaataaat
      541 cactgacggt tcaatatttc aacacaaccg aatcgcccgt agtctttgcc aggatgaggg
      601 gatacacggc actggcaaat atagcggtcg ggtttgcaat ttggcttatg tcgttcatgc
      661 ttccctacat gcaaatgttc ctcctgctgg gactgataat aagtctgact ggaataagca
      721 tgcttttcag caaccctgca ggaaaagaaa tgccgcacca gcataaaaag ctcattatta
      781 agaaaaaata ctggcttttc tatatgctga atttcctttc cggaacacgg cgccagatat
      841 tcacagtctt tgccatattc cttcttgtgg aacaatatca gctgagttta agggtggtaa
      901 caggtatcta tgtgattaac tatgccctta catacctgct gaatccacag atatccaggg
      961 cattgaacaa atatggcgaa cggattgtgc tttcactgga aagtgccagc cttatcgtgc
     1021 tttttctcgg atatgccttt ataaataatg tctgggtgat aataggcctg tttgtaatgg
     1081 acagtatatt tttcaatttc tcaattggtc taaatacata tctccagaaa actgccgatc
     1141 cggaagatct gggaccctcc actgccgtag gctttacgat aaaccatatt tccgcagtaa
     1201 taattcctgt tttcggaggt gcgctttgga tgcttaactg gagattacca ttccttgtcg
     1261 ggacaggatt tgcaatcctc tctctctact tcactcagct gattacaacc gactaccacg
     1321 aaaaaagagc tctttcattc attgcaaggc tgaggtagaa aaggctccct gtcctttcca
     1381 aaagcaatat cacggccaca cataatacca aaccatcccg gaactgtaaa cggatgattc
     1441 aacatcgtca gaaatcccag actcagggaa aaatttccat tatttttgta atttagctcc
     1501 tgatgaacag ggttttttta atagcatttc tttctgcatt tttctatttt cagctttctg
     1561 cccaggattt tactgacagc aatctgccaa ttgtaataat caataccgat catgggatgg
     1621 aaataccgga tgatccgcgg atcctggctg acatgaagat catttacagg ggccccggtc
     1681 tgaggaatta cgtttctgac cagaatactc ccggatacct gaattataac ggaaggatca
     1741 acatagaaat acggggttct tcaacccagg ttgcggaaaa aaagcaatac ggactgacca
     1801 ctcttaagaa tgacgacaag agtaacaata atgtaaaact gctgggcctt cccaaggaaa
     1861 atgactggat cctcaacggc atggtattcg atacggcgct catccgcgac tatctctcat
     1921 acaacctttc aaggcagatc ggtgaatacg cctcccgaac agtgtattgc gaggttatca
     1981 ttaacggcaa ttaccgcggc ttgtatgttc ttcaggaaaa aatcaaggct gatgaaaacc
     2041 gggtaaacat aatcaaaata ggggtcaacg ataattcgta ccctgaggtc accggtggtt
     2101 acattacaaa atctgataaa acgaccggcg gtgatccggt tgcctggacc ttgccttcat
     2161 ggtttgacac tccggttgat ttcattcatg aacttcccaa acctaagaat gtaacttcac
     2221 agcagaatga ttatatccgc agccggttcc ggcttcttca ggaaacatca atcgccggtg
     2281 atacttcgcc gttaacagga ttcccttcca taattgacat cacatcattc attgatttca
     2341 tacttctcaa tgaactggcc tccaatgccg acggatacat gtacagtaca ttcttccaca
     2401 aggaccggaa cggcaagctg agggcaggtc ccatatggga ttttgacctg acctacggga
     2461 acgatctttt cttctgggga tatgacagga gcaagacaaa tgtatggcag ttgtcaaacg
     2521 gggaaaacga cggggcaaga ttctggaaag agctttttga gaacccgtta ttcaggtgcc
     2581 atgcggcaaa aaggtggaac agccttacca ggcccggaca accttttaac caggaggtca
     2641 ttgaatcatt tattgaccgt acggttgaaa cgataagcga agccgttggc agggaatatg
     2701 ccagatgggg tcagaaagct gatcattcca ggctgatagc agaccttaaa ttattcattt
     2761 cccgcagaat tgcctggata tcggccaata tcggatcttc caccgcatgc gaaaatacac
     2821 cggttccgcc cctggtgata tcaaaaatcg attatcatcc cgaatcatca tccgaattcc
     2881 ccgacagtaa agatcttgag ttcattgaga tcaaaaacaa cggaaacagc agggttgacc
     2941 ttaccggtat ttatttcggc ggaacagggc tggttttcca gttccagcca tactcatccc
     3001 ttggtccgct tgaatcgata ataattgccg gaaattcagc agcattccgc ctgaaatacg
     3061 gattcacacc atttgcaacg ttttcaggaa gtctgcctga cgaaggaaag gaactggtgc
     3121 ttgccgacgc tttcggaaac atcattgacc tggtgagtta ttcgaacaag gccccatggc
     3181 ccgatgctga cggcaacggc agttacctga aactcacaga cccggatctc gataacaacg
     3241 atccgtcaaa ctggattgca acaagggaaa ccctggtcaa tgaaaatata atttctgctg
     3301 atccggccac tgtattattc ccaaatccag ttgcaaatat cctgaatgta agaaacaata
     3361 ccctgatcat ctcactgatg ctgtatgatg ttctgggccg tcctgttaaa atatcagaag
     3421 taaattcact tgaatgtcag cttgatgtta cagggcttcc atccggaact tacttcctga
     3481 agattgttac cctgtcagga acacttactg aaagactgct taaacactaa acggccggca
     3541 gggcgccggc attatttttt cttcagcgga tcctccgccc ttctatagaa ttcctcctgc
     3601 aactgttcaa tgctgttgtt gtttttgagg tacctgatca gttccacgat ctggtccgac
     3661 cagattttga aaatcagttc cccaagttct ttgtcaggca caacaacgtc gcctgcgaaa
     3721 tggttgggaa attttgcata ccaccagatc cccgtatagc caaaaggcat cttttcaagc
     3781 cgtttctgat caagtcctga ttcttttgtt atggctccga catccaccag gtcgggcctg
     3841 atagagtaca tcattgacgt ttcttcctcg cctgcatgtc cgtcgagttt ggccttcctg
     3901 agtttttcaa tctcttttga atgaacggga tcgtcttcct gtcggaataa aacaacaaca
     3961 taatcttttc ttgaggcgag ctgcgactgg cagaaatatt ccaggaaact tgtgctgccg
     4021 ccatgtccgt tgaccaggat gatttttttc aaaccattcc ttgccagttc ttcacaagtc
     4081 tcttccagca ttttccacat aagctcacta ctataggcta ttgtgcctgg ctggtgtttg
     4141 gcttcaaaaa tctgtcctgc aaaatacggt ggaaaaacca ctgcatattc cttttttgct
     4201 gcttccgtgg cagcctgcct ggcttcaaaa agatctgttc ccaatggcag atgcggtccg
     4261 tgcttttcaa ttatccccag cggaatgatg cagacccctt tcgaaagttc gagcgcctgt
     4321 acaaaacggg gtgaagacag ctcttccatt ttatacggca aagacggatg aggtatctgc
     4381 tgcccccata agaaggcagc aaacagcaag gaagtaaagg ttatcgttat ttttttcatg
     4441 gaggataaaa aggattaatt atggttctgt caaattaatt tctattcgat ggccgcaagc
     4501 ctgaaagggt gaatttcaat cttctgccag acgcccccgg ttatataggg ctcttccttc
     4561 agtctttttt caagagcctc cctgtcagga aattcataaa ccaccattga accgatcatc
     4621 cggccatcat cgtcaagaat ggctccgccg aaaagcatct ccccggtttt tttcagaatc
     4681 gcaattttcc cgaggtgctc ctggcgtatt ctcatccttc tctccagggc atccggatca
     4741 tttccgtcgt aagctacaag taaaaactgc atattatccg ttcactactg ttaattaatt
     4801 cacattgact atcaaattta agtaatttgg aattggaaca gcaaaccacc tgcacaaatt
     4861 aatgatccgt tttttatatt tgttccaggt ttcaggataa atttccggtt ttcaggatat
     4921 aacaaggtat ttaatgtcag gactcggaac aatgacaaaa catttccggt ttttgaagga
     4981 aaggaagaaa aaggttcata aaccggtatt tggtgctctt gatattctca ggtatatcgg
     5041 gcctggaatg cttgtgacgg taggctttat tgatcccggc aactgggctg caaacatagc
     5101 gtcaggatct gaattcggct ataccctcct ctgga
//
LOCUS       DAOOYG010000166         5117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_18931_length_5117_cov_4.654089, whole genome shotgun sequence.
ACCESSION   DAOOYG010000166 DAOOYG010000000
VERSION     DAOOYG010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18931_length_5117_cov_4.654089"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="PK288_10785"
     CDS             complement(<1..1031)
                     /locus_tag="PK288_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19455.1"
                     /translation="MKKACVLILLLMISLWISAQVRSDEKVNPGTDIQKDKPTLVWVK
                     YDFKPGSRVIFEDNHDNEINGEFPSRWEIIKGLGIENAEFDNNKVIYFRTGEAYIVPL
                     VSNPRGDYLPDQFTIEFDAWFEAREYCYYYLKLYDARNQSESCNLITIAANAIYYKGV
                     GGSTYPGERHDDNLERSYWRHLSLSFNGRGLKIYLDDTRLINIPNINCNPTGFTIGVP
                     SFNSAGTKGINRFIRNIRIAEVIAADNTNANTTYALTPGQETAIKSTTTDVLAGREPV
                     SMADKNTINNALNPVGEVTSVIPRNDDFVPGELFMSDARPKTWNIEWNSEDGLDFMAY
                     ASLMRAPYI"
     gene            complement(1095..>5117)
                     /locus_tag="PK288_10790"
     CDS             complement(1095..>5117)
                     /locus_tag="PK288_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPM19456.1"
                     /translation="PIVVKRNDQTHPSISRATKNPRSTIYITYEDQGDVYLYAPFLNE
                     GEGYRKMLPLDYGPDWTYQSNPQYENKQLVYVDYRPICDFIHRSIYVCQVSNIALSTT
                     THIKLVSSESGADQTNPRIYDDIVVWQYTKGSDSDIYIWKRPPGADLQLTVTESADPV
                     PAGDTLKYFLVLNNDGPIRRAPASVECTIPAEAIFVSATSGKGNVTIEGLKITWNIDS
                     LAYGSEDTLTIKMLTSGDCILTFNATASTPLFDPDPSNNKVSEKTKVKTIFPRSVDKG
                     TNAGMLVEPDGTVHLIYRKGDDYSQDGDLMYASKTRSGKWQYHKIGTCPPYISGFFRL
                     AMTSDRTIHVVYSNQDWSLTPKSRLYYGTLTPGRQWQSKIIAVSDSTFLFPTIDVTSH
                     DELWVAWIKASGLHGTVMIKNTRNGYWQAEKKVGGGYCHLDIAVDGSDNVHLCTYDVN
                     LGIIYYRVINGIPGPPEQVEPGWKGGQKEGLYTSIMTDKMNRPHISYVGQVNNDNREN
                     TKHAWKENNVWFNEKIDNGEYFSSINKVAFDPFNAVNLVYFHLPTYMTRFASNFSGNW
                     IKLPIDGSLNDLAMDASGNGHMIVNSIDYVFIPPLDYIKADPKVLDFEVVQPGASRTL
                     SLVLSNPALKEIRIDSIKSGDERFTVDKTSFVLDGFATDTVNVTFTQSITGAKADNLL
                     TIIYNIPAGLIMEIPLKAFSLAPRLQTDEETVDLGSVPLNSTATKTVILKNTGANDLI
                     FSNINVKYELFPGIPWATDFQLSGHNCSTLHYGETCQVEVSFSPHIEGEQFSYLNIYS
                     NDPEMPAKQIMLTGRTAYASIMPGKTSMNFGYCEAGQSKKDTLIIKNEGGAVLAVSGV
                     AITGANKELFSANNVCQNIQPGDSCIIVVTFAPVTGGDFQANLVINSNSLYSKTITIP
                     LAGSSILRNLTLSDSRIVFGEIPVGHKAFFRLALTNTGSNDVSINEIRVTGTDRYEFS
                     QGYASILCNVLQPGVTCTDTVYFIPLFEGEKNAILTVVSNDSYNPVQTVVLTGKAILN
                     NNSISGEIWDETGLRGINKSHVSLIPMADVADTTLLLLDGSNNYRFGGLPAGRYTVLA
                     TPDPVEYPDELPTYYGDNILLNNALWIQALVDITGSDIRLIKKPPTGAGTGIISGTFL
                     TGSSKGISVSEKSQDIKGNPLSGIYVYLKSASGGSLVSYDITGADGGFRFQGLEDGSY
                     IFLADYRGKAMDPANPALVINSGRREIEILATAGAETITINDLATGIDLIADAAIRIY
                     PVPASEKITIQIPDRLFSGKSIRLRIADLSGRYLYINEFHDLHDYNLTIDVSGIKDGL
                     YIIEIGNKEKTLKTRFLIMR"
BASE COUNT         1311 a         1086 c         1169 g         1551 t
ORIGIN      
        1 tgtatatatg gagcccgcat caaagaagca tatgccataa aatcaagccc atcctcactg
       61 ttccattcta tattccaggt tttagggcgt gcatctgaca taaaaagttc tcccggaacg
      121 aaatcatcat tgcggggaat aacggaagtt acctcgccaa cagggttgag tgcattgtta
      181 attgtatttt tatcagccat acttacgggt tctctaccgg caagtacatc tgttgtggtg
      241 ctttttattg cagtctcctg gccgggtgtc agagcatatg ttgtgtttgc atttgtatta
      301 tctgcagcaa ttacttcagc aattctgata ttcctgataa agcggttaat accttttgta
      361 ccggcagaat tgaacgaggg aacgccaata gtaaaaccgg tcggattgca gttgatgttc
      421 ggaatattaa tcagccgtgt atcatcaagg tatattttca gaccgcgtcc gttgaatgac
      481 aaggataaat gtctccaata acttctttca agattatcat cgtgtctttc tcccgggtat
      541 gttgaccctc ccacgccctt atagtaaatt gcatttgcag ctattgtaat caggttacag
      601 gattctgact gattcctggc atcataaagc ttcaggtaat aataacaata ttcccttgct
      661 tcaaaccatg catcaaattc gatagtgaat tgatcaggaa ggtaatctcc tcgcgggttt
      721 gataccagtg gaacaatata ggcttcacct gtcctgaagt aaatcacttt gttattatca
      781 aactcggcat tctcaatacc gagccctttg atgatctccc agcgcgaagg aaattctccg
      841 ttaatttcat tatcatggtt atcctcgaaa atgactctgc tgccgggctt aaaatcatat
      901 ttaacccaga caagagtagg tttatccttc tggatatccg ttccgggatt taccttttca
      961 tcggatctta cctgcgcgct gatccagagc gagatcataa gaaggaggat tagtacgcat
     1021 gcctttttca ttttctgttt cttattattt tcaactttca ataaaaattc aggatttgta
     1081 tctttaatcg cctttcatct cattattaag aacctggttt ttaaagtttt ctctttattt
     1141 cctatttcaa ttatatataa tccatctttt atgccactga catcaattgt aaggttataa
     1201 tcatgtaagt catgaaattc atttatatac agatatcttc cggataaatc ggctatcctg
     1261 aggcgtattg attttccact gaataatctg tcaggtatct gaatggtaat cttttccgat
     1321 gcgggaaccg ggtaaatcct gattgcagca tcggcaatca ggtcaatacc tgttgcaagg
     1381 tcattaattg tgattgtttc agctcctgcc gtggccagta tttctatttc ccttctgccg
     1441 ctgttaatta ccagggcagg attggcagga tccattgcct taccacggta atcagccaga
     1501 aagatatagg agccatcttc cagaccctgg aacctgaacc ctccgtcagc acccgttatg
     1561 tcatatgata ccaggctgcc cccggaagcg cttttgagat agacataaat accggacagt
     1621 ggattacctt taatatcctg cgatttttcc gaaacagata ttccttttga ggaaccggtt
     1681 aaaaaagtac cactgataat tcccgttccg gctccggtgg gaggtttctt aataagccgg
     1741 atatcactgc ctgtaatatc aaccagagcc tggatccata aagcgttatt gagcaggata
     1801 ttatcaccgt aataggtcgg gagttcatcc ggatactcaa ccggatcggg tgttgcaaga
     1861 acggtgtacc ttcctgccgg aagtcctccg aagcggtaat tgttcgatcc gtcgagcaaa
     1921 aggagggttg tatctgcaac atctgccata gggataagag atacatgtga tttattgata
     1981 ccccgtaaac ctgtttcatc ccatatttca cctgaaatac tgttgttgtt gagtattgct
     2041 ttccctgtga gtactaccgt ttgcacggga ttgtatgagt catttgaaac gactgtgagt
     2101 atcgcattct tttctccttc aaaaagggga ataaaataaa ccgtatcggt acacgtaaca
     2161 ccgggttgca aaacgttgca caagatggaa gcatacccct gagagaattc ataccggtca
     2221 gttccagtaa cccttatttc gttgattgat acatcattgg agccggtatt ggttagtgct
     2281 aacctaaaaa atgccttatg tccgacagga atttctccga aaactattcg tgagtctgac
     2341 agggtaagat ttctcagaat acttgaaccc gcaaggggga tggtaattgt tttggaatag
     2401 agtgaattgg aatttatcac aagattagcc tgaaaatcgc ctccggtaac cggagcaaat
     2461 gttactacta taatgcatga gtcgcccggc tgaatattct ggcatacgtt gtttgcgctg
     2521 aaaagctcct tgtttgctcc ggtaatggcg acacccgata cagcaagaac tgctcctcct
     2581 tcgtttttta ttataagggt gtcttttttc gactggccgg cttcacaata tccgaagttc
     2641 atggaagttt taccgggcat gatggatgca taggcagtcc ttccggtcag catgatctgc
     2701 tttgcaggca tttccgggtc gttggagtag atgttcagat aggagaactg ttcgccttca
     2761 atatgtggac tgaacgaaac ttcaacctgg caggtttcac cataatgaag ggtggaacag
     2821 ttgtgtcctg aaagctggaa atcggtagcc cacggaatcc ccgggaatag ttcgtactta
     2881 acatttatgt tgctgaatat taaatcattg gctccggtat ttttcagaat gactgttttt
     2941 gtcgctgtgc tgttcagagg aacggatcca agatcaacag tttcttcatc tgtctggagg
     3001 cgaggagcaa gggaaaatgc cttcagcggt atttccatta tgaggcctgc ggggatgttg
     3061 tatatgatgg tcagcagatt atcagccttt gcccccgtaa tgctttgtgt aaaggtaaca
     3121 ttgacggtat ctgtagcaaa accatcaagc acgaaggagg ttttatcaac cgtaaatctt
     3181 tcatccccgg atttgatgct gtcgattctt atttctttca gggccggatt ggagaggaca
     3241 agtgacaggg tgcggctggc tcctggctga acaacttcga aatcaagtac cttcggatcg
     3301 gcttttatgt aatccagagg gggaataaat acataatcta tactgttaac aatcatgtgt
     3361 ccgttcccgg aggcatccat ggcaagatcg ttcaatgagc cgtcgatggg cagttttatc
     3421 cagtttccgg agaagttgga cgcaaaacgc gtcatgtaag ttggaagatg aaaatagaca
     3481 agattgacag cattgaacgg atcgaaggca accttgttaa ttgaactgaa atattccccg
     3541 ttgtctattt tttcgttgaa ccagacatta ttttccttcc atgcatgctt ggtgttttcc
     3601 ctgttatcat tatttacctg tccgacatat gagatgtggg gacggttcat tttgtcggtc
     3661 atgattgagg tgtacaatcc ttctttttgt ccgcctttcc agccaggttc aacctgttcg
     3721 ggcggtcccg gaatcccgtt tattacccga taataaatta ttccaaggtt aacatcatag
     3781 gtacacagat gtacattgtc cgaaccgtca accgcaatat ccagatggca gtatccgcct
     3841 ccgaccttct tttctgcctg ccagtatccg ttacgggtgt ttttgatcat aactgtccca
     3901 tgtaatccgg atgctttaat ccaggccacc catagttcat catgcgaagt aacatcaatt
     3961 gtgggaaaca ggaaggttga gtcggagact gcaatgattt ttgattgcca ttgacgtccg
     4021 ggtgtcaggg ttccgtaata aaggcggctc tttggtgtga gagaccagtc ctgattggaa
     4081 taaacaacat ggatagtcct atcgctggtc atagcaagcc ggaagaaacc ggatatgtaa
     4141 ggagggcagg tgccgatttt gtgatactgc catttgccgc tgcgggtttt ggaggcatac
     4201 ataaggtcgc catcctggct gtaatcatct cctttccggt agatcagatg gactgttcca
     4261 tcgggttcca caagcatgcc tgcatttgtt cccttgtcaa cggagcgggg gaagattgtt
     4321 ttgacttttg ttttttcgct tactttattg ttcgaaggat ccggatcaaa aagcggagta
     4381 ctggctgtgg cattgaatgt gagtatacag tcgcctgatg tcagcatctt tattgtcagg
     4441 gtgtcttccg atccgtatgc caggctgtcg atgttccagg ttatcttaag gccttcgatg
     4501 gttacatttc ccttgccgga ggtggcagaa acaaagattg cttcggcggg gatggtgcat
     4561 tcaacgctgg ccggtgcacg tctgataggg ccgtcattgt tgagtacaag aaagtacttc
     4621 agtgtgtcgc ctgccggaac agggtcggcc gactcggtta ctgttaactg aaggtcggca
     4681 ccgggaggcc gtttccagat gtatatatct gaatcggatc ctttggtgta ttgccagaca
     4741 acgatatcgt catatatcct gggattggtc tggtctgcac cactttcaga actgacaagt
     4801 ttaatatgtg tggttgtgga gagagctata ttgctaacct gacagacata tatgctacga
     4861 tggataaaat cacatatagg cctataatca acatatacca gttgtttgtt ttcatactgt
     4921 ggattagact gataagtcca atccgggcca taatcgagag gaagcatttt tctgtagcct
     4981 tctccctcat taagaaaggg cgcataaagg taaacgtctc cctggtcttc atatgtaata
     5041 tatatggtcg aacgagggtt cttggtagcc cttgaaatag aagggtgtgt ctggtcattt
     5101 cttttgacaa caatggg
//
LOCUS       DAOOYG010000167         5105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_18987_length_5105_cov_7.985545, whole genome shotgun sequence.
ACCESSION   DAOOYG010000167 DAOOYG010000000
VERSION     DAOOYG010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18987_length_5105_cov_7.985545"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..1131
                     /locus_tag="PK288_10795"
     CDS             295..1131
                     /locus_tag="PK288_10795"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPM19457.1"
                     /translation="MKKENHNRTLISNVSGRPFEIKNFDDLVFEHRNRDYGAYQLRKR
                     YPRVLFAAILISIAIAATVVFIPFLFRSKQEIAFGGGRGGVMISLENLAPPEEQIFIP
                     PAAPPPPQPERMQEMIEYIPPVVVDSILPNEATLLSAEDILSSVDSEITDVAGYGFGE
                     GSGSGPGSGGGDLEEPLFQVEVMPSFRGGDLSKFREWVGKRTNYPQAAIQNKIRGTVY
                     LTFIVEKDGSVSSVTILKGVHPLLDNEAVKVISESPKWTPGLQRGQPVRVRFQIPLSF
                     IY"
     gene            1219..2391
                     /locus_tag="PK288_10800"
     CDS             1219..2391
                     /locus_tag="PK288_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19458.1"
                     /translation="MRKIAPAFMILIITLSGCGSSKKQLEKGNYDAAIERAVKQLRKD
                     PRDAKQIAILDKSYRVANEQDNERIRFLKMESLPANWDEIYLINKRLSDRQSLVRSVT
                     PLELNGKSISYEYIDYMPEMIAAKRKSADYYYAHGNELMKNNQKESFRQAYYEFVRAK
                     EYVGDYENIDNKIEESKYLGMSRVVISLINKSMLKFTPEFEEDLLALDLPRLNSEWVE
                     YHIMNYNNNLKYDYFININVKTIAVSPDQTMQRDSVIKRDVEDGFNYQVDSRGNVMKD
                     SLGNDIKTKRYKTLQCALIETVQSKSCVIEGDIEILQSSPGKLLKKEPLGASSHFENI
                     SARAVGDINALNKFQLQKTKSSPVPFPDDMEMIFRCSESLKRAINGAVQNNRRLIN"
     gene            2392..3588
                     /gene="hflX"
                     /locus_tag="PK288_10805"
     CDS             2392..3588
                     /gene="hflX"
                     /locus_tag="PK288_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPM19459.1"
                     /translation="MIPTGKKAERAILIGLIYPGQDENQARDYIAELSFLAKTAGAVP
                     VRSFMQRMPAPDPRTFVGSGKIAEIAACVSENNIDIAVFDDELSPGQIKNIETALGCR
                     ILDRTNLILDIFARHARSSHAKTQVELAQYQYLLPRLAGMWTHLERQRGGIGLRGPGE
                     TEIETDRRIIRDKIALLKSRLRKIDKQMATQRKNRGKRVRVALVGYTNAGKSTIMNML
                     SKSEVFAGDKLFATLDTTVRKVVIENLPFLLSDTVGFIRKLPHDLVESFKSTLDEVRE
                     SDLLLHVVDISHPGFEEQISVVSQTLSELNSTDKPVIMVFNKIDSFSWTIREEDDLTP
                     PGKENYSLDDLKRTWMASDKSGPTVFISAKTGENSGELRKLLLEEVRKIHFTRYPYCD
                     SRYASN"
     gene            complement(3623..4612)
                     /locus_tag="PK288_10810"
     CDS             complement(3623..4612)
                     /locus_tag="PK288_10810"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPM19460.1"
                     /translation="MKVAVVGATGLVGKTMIQVLEERNFPVSQLIPAASEKSVGKEIQ
                     FRGETVKVVSVSDAVKAKPVFAIFSAGASTSKEWAPVFADNGIVVIDNSSAWRMDNKV
                     PLIVPEVNGHAIKKGDRIIANPNCSTIQLVMALAPLHRKYKVKRLVIATYQSVTGTGV
                     KAVQQMENERRGIKGEMAYAHQIDLNCFPHGGSFQSDGYTTEEQKLLDETRKILEDQS
                     IMVTATVVRIPVVGGHSEAVNIEFENEYDIEDVKTLLGQFPGVVIYDDPSQAKYPMPI
                     LSHNRDEVFVGRIRRDHSKEKCLNLWIVSDNIRKGAATNALQIAEYMIEKKLY"
     gene            4704..>5105
                     /locus_tag="PK288_10815"
     CDS             4704..>5105
                     /locus_tag="PK288_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19461.1"
                     /translation="MKKVFLLALLLPVMASGQIMEFFESDDISRWIQIPEGRWNCDNI
                     DAISGTRSLHHCFDNPGSGLDCAGLRLNDLYPGEGPVKWSIKVRHGTEPSSLNNWTVF
                     LVSDSDPGSVLSDRKERTLPGSESETRKTVQL"
BASE COUNT         1451 a         1117 c         1162 g         1375 t
ORIGIN      
        1 cccctgacct ttagactgcc agtctaacgc tctagccaac tgagctaaac ccccttcagg
       61 atgtcaaacg tatcgaggaa accaattgaa ccaaacctgg cccaaaagcc tggccaaatg
      121 taagaaaaaa acggataatt aaaaaacatc atttctcttg tgtcgtctta actttgatga
      181 gaaacagtat tttaatatct tcgcatttaa ttttttaaaa aacaggtcag attttgcaaa
      241 ctgacctgat tgttggcaca gtctttggaa acgcagaggg cttggcttat tcaggtgaaa
      301 aaagagaatc acaataggac ccttatcagt aacgtctcgg gcagaccatt tgaaattaag
      361 aacttcgatg accttgtttt tgagcacagg aacagggact atggggctta tcagctccga
      421 aagcgatatc cgagagtgtt gtttgccgcg attcttattt ccattgctat tgctgcaaca
      481 gtagttttta tccctttcct tttcaggtcg aaacaggaaa tagcctttgg gggtggcagg
      541 ggcggagtca tgatcagcct ggaaaacctt gcaccgcccg aagaacagat ctttattccc
      601 ccggcagcac caccgcctcc ccagcctgaa agaatgcagg agatgatcga atatattcct
      661 ccggttgttg tagattcaat tttacccaac gaggcaaccc ttttatcagc agaagacatt
      721 ctttcatcag ttgatagcga aattactgac gtggccggct atggttttgg tgaaggttcc
      781 ggctcaggcc cgggttcagg gggtggagat cttgaagagc cgcttttcca ggttgaggtt
      841 atgccatcct tcaggggggg agatctttcg aaattcaggg aatgggttgg caaaagaacc
      901 aattacccac aggcagctat acagaataaa ataaggggta ctgtttatct gaccttcatt
      961 gtagaaaagg atggatcggt cagcagtgtt accatcctga aaggagttca tccgcttctg
     1021 gataatgaag ctgtaaaggt aatatccgaa tcaccgaaat ggaccccagg actgcaacgg
     1081 ggacagccgg tcagggttcg tttccagata cccttgagtt ttatttactg atatgacaca
     1141 aatttcaatt ttaagcgttt taattatcag tatcctatga tatcaagtga agctatctta
     1201 actttaataa aaaaagacat gagaaaaatt gctccggctt ttatgatttt aattattacg
     1261 ctttcaggat gcggctcatc caaaaagcag cttgaaaaag gcaactatga tgctgccatt
     1321 gaaagggcgg ttaagcagct tcggaaggat ccccgtgatg ccaaacagat tgccatactt
     1381 gacaaatcat acagggtggc aaatgagcag gataacgaaa gaatccgttt cctgaaaatg
     1441 gaatcgctac cggccaactg ggacgagatc taccttataa ataagcggct tagcgacagg
     1501 caatcccttg tcagatcagt tactcccctg gaacttaacg gaaaatcaat ttcatatgaa
     1561 tatattgatt acatgcccga gatgatagct gccaaaagaa aatcagctga ctattattac
     1621 gcacatggga atgaacttat gaagaataat cagaaggaat ctttccggca ggcttattat
     1681 gagtttgtaa gagcaaaaga atatgtcggg gattacgaaa atatcgacaa taagattgag
     1741 gaatccaaat atctgggaat gagcagggtt gtgatttcac tgattaataa atcaatgctg
     1801 aaattcactc ctgaatttga ggaggacctt ctggcgctag atctcccgcg gctcaacagc
     1861 gaatgggttg aataccatat tatgaattat aacaacaatt taaaatacga ctatttcatc
     1921 aatatcaatg tgaagaccat tgcagtaagc ccggatcaga ccatgcagcg ggattctgtg
     1981 ataaagaggg atgttgaaga cgggttcaat taccaggttg acagcagggg aaatgttatg
     2041 aaggattcac ttggcaatga tattaaaacg aaaagataca agaccctgca atgtgcactc
     2101 attgaaacgg ttcagtccaa atcctgcgtc attgaagggg atattgaaat actccagtcc
     2161 agtccgggaa agcttcttaa aaaagagccg ctcggtgcca gttcgcattt cgagaatatt
     2221 tcagctcgtg cggtgggaga tataaatgca ctgaacaaat tccagcttca aaaaaccaaa
     2281 agcagcccgg ttccttttcc tgatgacatg gaaatgattt tccgatgttc ggaatcactg
     2341 aaacgggcaa taaacggagc ggtgcaaaat aacaggaggc tgataaacta aatgattcca
     2401 acgggaaaga aagcagaaag ggccattctg atcggattga tctaccccgg gcaggatgag
     2461 aaccaggcac gtgattacat tgcagagctt tccttcctgg caaaaacagc tggtgcggtt
     2521 ccggtacgta gtttcatgca gagaatgcct gcacctgatc cgcgtacctt tgtcggttca
     2581 ggcaaaatag cagagattgc tgcctgtgtg tcagaaaaca atatcgatat cgccgttttt
     2641 gacgatgaac tctcgccggg tcagataaag aacattgaga cggcactggg atgccggatc
     2701 cttgaccgga ccaatctgat actggatatt tttgcaagac atgcaagaag ttcacatgca
     2761 aaaacacagg ttgagcttgc ccaataccag tatcttctgc cccgccttgc gggaatgtgg
     2821 acccatctcg aacgccagcg tggcggaata ggactcaggg gacctgggga aaccgagatc
     2881 gaaactgaca gaaggatcat cagggataaa atagccctcc tgaaatcgag gctcaggaaa
     2941 atagacaaac agatggcgac ccagcgcaag aaccgcggaa aaagggttag ggtggcactg
     3001 gtgggttata caaatgcagg taagtcaacc atcatgaata tgctcagcaa gtcggaagta
     3061 ttcgcaggag ataagttatt tgccaccctt gatacgaccg taaggaaagt cgttattgaa
     3121 aatctgcctt ttcttttgtc ggataccgtc gggttcattc gcaaactgcc tcatgacctt
     3181 gttgaatcgt ttaaatcaac tctcgatgaa gtcagggaat cggatctcct gcttcatgtg
     3241 gtcgatattt cacatccggg ctttgaggag cagatttcgg tggtcagtca gacactcagc
     3301 gaacttaatt caacagataa gcctgtaatt atggtgttta ataagatcga cagtttttcc
     3361 tggacgatca gggaagaaga cgatctgaca cctccaggga aagaaaatta ttcccttgat
     3421 gatcttaaga gaacctggat ggcttcagac aagagcggtc cgaccgtatt catctcggca
     3481 aaaacgggtg aaaactccgg tgaactcaga aaactgctcc tggaagaggt aaggaaaata
     3541 catttcacac gctatcccta ttgtgattcc cggtatgcat cgaattgaac ttcaagttct
     3601 ttccgtgcaa atggaaatct tttcaataga gctttttctc aatcatgtat tctgcgatct
     3661 gaagtgcatt ggttgctgcc cccttgcgga tattgtcaga aacgatccaa aggttgaggc
     3721 atttttcttt tgaatggtcc cttcttatcc ttccgacaaa cacctcatcc ctgttgtgtg
     3781 ataatatcgg catcggatac tttgcctggg aaggatcatc atatattacc actccgggaa
     3841 actggcccag cagtgttttg acgtcttcaa tgtcatactc gttctcaaat tcaatgttga
     3901 cagcttcgga gtgtcctcca actacgggga tcctgacaac ggtggctgta accattatcg
     3961 actgatcttc aagtatcttg cgtgtttcat caagcaattt ctgctcttcc gtggtgtatc
     4021 cgtcactctg gaaggacccg ccgtgaggaa aacagttcag gtctatctga tgggcatagg
     4081 ccatctcacc ctttattccc cggcgctcgt tttccatctg ctgcacagcc ttcactcccg
     4141 taccggtaac tgactgatat gttgcaataa caagcctttt taccttatat ttcctgtgga
     4201 ggggcgccag tgccattacc agctgaatcg ttgaacagtt tggatttgca atgatccggt
     4261 cgcctttttt tatcgcatgt ccgtttactt cgggaacaat cagcggaact ttattgtcca
     4321 tccgccatgc ggaggaattg tctataacaa caattccgtt gtcggcaaat accggagccc
     4381 attccttcga tgtcgaagcg ccggcggaaa atatggcaaa gacaggtttc gcctttactg
     4441 cgtcggaaac acttactacc tttacggttt ctcccctgaa ttgtatttcc ttacctactg
     4501 atttttccga tgctgcagga ataagctgag atacgggaaa atttctttcc tcaagaacct
     4561 gaatcattgt ttttcctacc aaccctgtgg ctcctactac ggctactttc atttttttat
     4621 ttgttaacga caaatcaatt ttttatattt actgttaaga aaaagctgtc aaaagtaatc
     4681 tttttttcaa tttgtcccgc tgcatgaaga aagttttttt gctggctttg ctgttgcccg
     4741 ttatggcatc cggccagatt atggaatttt ttgaatccga tgatatttcc cgctggattc
     4801 agatacctga aggtcgctgg aactgtgata acattgatgc aatctcaggt acccgctcgc
     4861 ttcatcattg ttttgataac cccggctcag gtcttgattg cgccgggtta cgcttaaatg
     4921 acctgtaccc cggggaagga ccggtaaaat ggagtataaa ggtccgccat ggaactgaac
     4981 catcatcttt gaataactgg acggttttcc tggtctccga ttcagatccc ggcagtgttc
     5041 tttctgaccg caaagaaaga acactgccgg gatctgaatc ggagaccagg aaaaccgtcc
     5101 agtta
//
LOCUS       DAOOYG010000168         4893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_20095_length_4893_cov_8.211658, whole genome shotgun sequence.
ACCESSION   DAOOYG010000168 DAOOYG010000000
VERSION     DAOOYG010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20095_length_4893_cov_8.211658"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..3193
                     /locus_tag="PK288_10820"
     CDS             68..3193
                     /locus_tag="PK288_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM19462.1"
                     /translation="MAQQRTVTGTVRDTDGAALSGVTVLVKNSTTGTITDAQGKYTIQ
                     AGPANVLVFSFIGYASQEITVGSQSTVNVTLALETEQLGEVVVTALGVSRSTKALQST
                     VAKVGGENLTQAREINLGSSIQGRVAGVNVSNAGTGPAGSSRVIIRGNKSIGGANQPL
                     YVVDGIPMDNSQFGRAGVWGGVDQGDGMSSINPDDIESITVLKGAAAAALYGSRGGYG
                     VINIVTKRGQARKGIGIEFNSNYVFEKVNNFSDLQTVYGPGGLADDPANPGTQIYSKA
                     VDRRQAYGWGAQSMWGPKFDGSSVVQFDNVKRPYSYVGNSWDFYKTGGSWTNSIALSG
                     GSDKQTFRFSFSDLDNKAVVPNETFKRRNLSLATNGKYGEKLTFNAKVLYSNEKVNNR
                     QYVADSPANIPQAIWGLATSVDIETLKGDPNKLGAVAAGVTTHDLKSTGEEFQQADNN
                     WLQNPYWVAYQFKNENVRDRFITSGTLRYDIFKWLYIQGQIGMDWYTRNNNLLVPQGT
                     GYLRGGSKNETSNFIREINQEWMLGGNKQFGKFGVNAFVGGNKMVRESESINANGNGF
                     NVPFFTSVNNAKDKTIGYGYSKSGINSLFGSAELSYGNYLFITATARNDWFSVLNPAN
                     NSKLYPSLGGSFVFSDAFPNLPEWLTYGKLRAAWGQVATANVGAYSSRVTYGLSGVSH
                     LTLAMANFSGGDNIPNPNLSPALSTELEFGTDLRFFKGRVGLEFTYYDQKTTDDILSA
                     TISRASGFLSTSVNIGELSNKGIEFLITATPVQGDFTWDISLNLAKNKNKVLALSENV
                     TELNVEEPRTRTVMIKHIVGQPFGMITGYKQKTDPQGNLVFEETGSPARSDVYEILGN
                     GVADLTGGLNNSFSWKRIRLDALLDFKFGGDIYSGTNVRLTQWGLHKQTLVNREGGMV
                     VKGVIKTGEDTNGKPIYEPFTKTLNQRETADYWGNLGERCQENFIYDASFIKLRQISL
                     EYSIPPAWLSKLPFTNLAVSFVGRNLAILYKNIDNVDPESNYTDSNAQGLDYFGMPTT
                     RTYGFNLRVTF"
     gene            3214..4830
                     /locus_tag="PK288_10825"
     CDS             3214..4830
                     /locus_tag="PK288_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPM19463.1"
                     /translation="MKNLYKLLGSALLIAFFAAGCDTDTLQDMNLNPNAVNTIDVNYF
                     LTAAELSASNNGLRDDRYTDWRTNIGMCAHAIQHLASTSTGTISVGDKYLENDVEPSN
                     APWDHFFAGDGRVTAEIIKQTGPGGYAEGHNINTRHAARILRVLCFHRLTDWYGSIPY
                     FEANKGIEGIFQPVYDKQKAIYTDLLKELDEACAGLSTSNLDEGFKAADYYFEGDIAK
                     WKKFGYSLMLRLAMRVSNVDKPLATTYVTKAVAGGVMSSNDDNVIATMALGPSEWTNQ
                     NGISRGFYPGDGGQWSSSFLSKTLVDKLMGTNKLSTADDDPRLMIFTGGIIQWTATSV
                     VYNQTDPLKQRGLPNGLDGAMLDAMEGGPTTPYEKFSAVNPLMMQDDEPYMIMHAAES
                     EFLLAEALKSGIGTGITGTVKSHYDNGVKLALQMYTIYDPSFAVSDARVTQYLTNYPF
                     DDAKALEMIGEQMWLSKWMNWWDAWSDYRRTGFPVLTPTNYPGNATNGQIPTKLTIPA
                     YEATGNPNYATGATQPDRLSGKVWWDGGPE"
BASE COUNT         1418 a         1022 c         1187 g         1266 t
ORIGIN      
        1 aaaaatgtac ctttttttga agcgggtact gactttgttt ctgctgctgt tatgcactag
       61 tgcattgatg gcgcagcaac gaacagtaac cgggactgtc agggacactg acggtgcagc
      121 actttcagga gtgactgttc tggtaaaaaa cagtacgacc gggacgatca cagatgctca
      181 gggcaagtac actattcagg caggacctgc caatgtgctt gtcttttctt ttatcggtta
      241 tgcctcccag gaaattactg tgggcagcca gtcgacagtc aatgtaactc tggctcttga
      301 gaccgaacag cttggtgagg ttgttgtgac tgctcttggt gtttcgagaa gcaccaaagc
      361 gctccagtca actgttgcaa aggttggagg tgagaacctt acccaggcca gggagatcaa
      421 ccttggttca tcgatccagg gacgcgtggc aggtgttaat gtttctaacg caggaacagg
      481 tccggccgga tcttcccgtg ttataatcag gggtaataaa tcgatcggag gagctaacca
      541 gcctctgtat gttgtcgacg ggatcccgat ggataactcc cagttcggcc gtgcaggtgt
      601 ctggggcggt gttgaccagg gcgatggtat gtccagtatc aaccccgatg atatcgaatc
      661 gatcactgta ctgaagggtg ctgcagcagc tgctctttat ggctcacgtg gaggttacgg
      721 tgttatcaac attgtaacta aaagaggtca ggcccgtaaa gggattggca ttgaattcaa
      781 ctccaattat gtatttgaaa aggttaataa cttttcagat cttcagaccg tttacggacc
      841 cggcggatta gctgatgatc ctgcaaatcc tggaacacaa atttactcaa aggcagtaga
      901 caggaggcag gcctatggct ggggagcaca atctatgtgg ggtccgaaat tcgacggaag
      961 ctcggtagtc cagttcgata atgtcaaaag gccatattct tatgtaggta acagctggga
     1021 cttttacaag acaggaggtt cgtggactaa cagtattgcc ctttcaggag gaagtgacaa
     1081 gcagactttc aggttctctt tctcggatct ggacaataaa gccgttgtac ctaacgagac
     1141 ctttaagaga aggaatctct cacttgctac caacggaaaa tatggtgaaa agcttacttt
     1201 caatgccaag gttctttatt caaatgagaa agtgaacaac cggcagtatg tcgccgatag
     1261 tcctgcaaat attcctcagg caatatgggg attagcaact tcagtggata ttgaaaccct
     1321 gaaaggagac cccaataaac tgggagcagt ggcagctggt gttacaactc atgacctgaa
     1381 atcaaccggt gaagaattcc agcaggctga taacaactgg cttcagaatc catactgggt
     1441 ggcctatcag ttcaagaacg aaaatgtcag ggaccgtttt ataacttcag ggaccctgcg
     1501 atatgacatt tttaaatggc tttatatcca gggacagatc ggtatggact ggtacaccag
     1561 gaacaataat ctccttgtcc cgcagggtac cggttatctt cgcggaggtt ctaaaaatga
     1621 aacttcaaat ttcatcaggg aaattaatca ggagtggatg cttggtggaa acaaacagtt
     1681 cgggaagttc ggagtaaatg catttgttgg cggcaacaag atggttagag agtctgaaag
     1741 tattaatgca aacggtaacg gttttaatgt tcctttcttt acatcggtta ataatgccaa
     1801 agacaagacc atcggttatg gttacagcaa atcaggtata aactcattat ttggttcagc
     1861 cgaattgagc tatgggaatt acctgtttat tacagcaacc gcaagaaatg actggttctc
     1921 ggtacttaat ccggcaaaca acagtaagct ctatccatct ctgggtggaa gctttgtctt
     1981 ttcagatgca ttcccgaatc tgccggaatg gctgacttat ggtaaactaa gagctgcatg
     2041 gggccaggtt gctacagcaa acgttggagc ttactcatcc cgagttactt acggattgtc
     2101 gggggtatct catcttactc ttgcaatggc aaacttctca ggtggtgaca atattccgaa
     2161 cccgaacctt tcgccggctc tgtcaaccga actcgaattc ggaacagatc taaggttctt
     2221 taaggggaga gttggcctcg agtttacata ttatgaccag aaaacaactg atgatatact
     2281 ttctgcaaca atctccagag catccggatt cttgtctaca tcagtcaaca tcggagaatt
     2341 atcaaacaag ggaatagaat ttctgattac tgctactcct gtacagggag actttacctg
     2401 ggatatatca cttaaccttg caaagaacaa gaacaaggtt ctggctctaa gtgaaaatgt
     2461 tactgaactt aatgtggaag aaccacgtac aaggacggtt atgatcaaac atattgtagg
     2521 gcagcctttc gggatgatta ccgggtataa gcaaaaaact gatcctcagg gaaaccttgt
     2581 gtttgaagaa accggctcac ctgcaagatc cgatgtttat gaaattctag gtaatggtgt
     2641 tgcagacctt acaggtggtc tgaataactc attcagctgg aaacggatac ggctggatgc
     2701 actccttgat ttcaagttcg gcggagatat ctattccgga accaatgtaa ggctgaccca
     2761 atggggattg cacaaacaaa ctctggtcaa ccgtgaagga ggaatggttg taaaaggtgt
     2821 tatcaagacg ggagaagata caaatggtaa accaatttat gaaccattta caaagactct
     2881 gaaccagagg gaaacagcag attactgggg caatcttggt gaacgttgtc aggagaattt
     2941 catttacgat gcttcattta taaagctgag gcaaatatct cttgagtaca gtattccgcc
     3001 agcctggctt agcaaattgc cattcaccaa ccttgcagtt tcatttgtag ggagaaacct
     3061 tgcgatattg tataaaaata tcgataacgt tgaccctgaa tccaattata ccgacagtaa
     3121 tgcacagggt cttgattact tcggtatgcc tacaacacgc acctatggat tcaatctgag
     3181 agtgactttc taacctttta aattttaaga aaaatgaaaa atctatataa attattagga
     3241 agtgcactgc tgatagcgtt tttcgcagca ggatgtgata cagataccct gcaggatatg
     3301 aaccttaatc ccaatgcagt aaatacaata gacgttaact atttccttac tgccgcggaa
     3361 ctgagtgcct ccaacaacgg actgcgtgat gacaggtata ccgactggag aacaaatatc
     3421 ggtatgtgcg cacatgcaat tcagcacctg gcaagtacaa gcacaggtac tatatcagtg
     3481 ggagacaaat acctcgagaa cgatgttgaa ccatcaaatg caccatggga ccatttcttt
     3541 gccggtgacg gaagggttac cgctgaaata atcaaacaga caggtcccgg tggctatgct
     3601 gaaggacata atatcaatac acgtcatgcc gcaaggatcc tcagagttct ctgtttccac
     3661 cgtcttaccg actggtatgg aagtatccct tattttgaag ccaataaagg tattgaaggc
     3721 atattccaac ctgtatatga taagcagaaa gcaatttata cagatctgct gaaggaactt
     3781 gatgaagcat gtgcaggatt gagcacctca aacctcgatg aaggattcaa ggctgcggac
     3841 tattacttcg agggggatat cgccaaatgg aaaaagttcg gctattccct gatgcttaga
     3901 cttgcaatga gggtttcaaa tgttgataag cctctggcga ccacttatgt tacaaaagct
     3961 gttgccggcg gggttatgag cagcaatgat gataatgtaa ttgctacaat ggctcttggt
     4021 cccagtgaat ggaccaacca gaacggcatc tcacgcggat tctatccggg tgacggaggc
     4081 cagtggtcca gctcattcct cagcaaaact cttgttgaca aactaatggg tacgaacaag
     4141 ttgagtacgg ctgacgatga tcccagactt atgatcttta cggggggtat catccagtgg
     4201 acagccacaa gtgtggtata taatcagaca gatcctctta agcaaagagg gcttccgaac
     4261 ggacttgacg gtgccatgct tgatgctatg gagggcggtc cgacgacacc ttatgagaaa
     4321 ttctcagctg tgaatcctct tatgatgcag gatgatgaac catacatgat aatgcatgct
     4381 gccgaatccg aattcctgct cgcagaagct cttaaaagcg ggatagggac cggaataacc
     4441 ggaacagtta aatcgcatta tgataacgga gtaaagcttg ccctgcagat gtatactatt
     4501 tatgatccgt catttgcagt atcggatgca agagtaaccc agtaccttac taactatccc
     4561 tttgatgatg ccaaagctct tgaaatgatc ggcgagcaga tgtggttatc caaatggatg
     4621 aactggtggg atgcatggtc agactacagg agaacaggtt ttcctgtcct gactcctacg
     4681 aattatcctg gtaatgcaac aaacgggcag ataccgacca agcttacgat acctgcatat
     4741 gaagctacag gtaatcctaa ctatgccaca ggcgccacac agcccgacag gctgagtggt
     4801 aaggtttggt gggatggcgg tccggaatga tgaattttcc gatcaaagat ttaatttaat
     4861 tgaaggaggc tggcattgtg tcagcctctt ttt
//
LOCUS       DAOOYG010000169         4737 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_21002_length_4737_cov_5.789193, whole genome shotgun sequence.
ACCESSION   DAOOYG010000169 DAOOYG010000000
VERSION     DAOOYG010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21002_length_4737_cov_5.789193"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK288_10830"
     CDS             <1..188
                     /locus_tag="PK288_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19464.1"
                     /translation="KSPNTLILHLVGPPENDNAGYCFKMLIGTDDSEMYYYHQQLIDK
                     ANPRGLLPADLKRMGKT"
     gene            240..596
                     /locus_tag="PK288_10835"
     CDS             240..596
                     /locus_tag="PK288_10835"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPM19465.1"
                     /translation="MKEIRELSESERNWAMFCHLSAYAGFFFPFGGIIGPLICWLSRR
                     DESQWINQNGKRSLNFQLSVLLYTVLALPLCIIIVGIPIIMALWLLKIICIAIASVKA
                     SKGEDFRYPLAIPFIQ"
     gene            863..2233
                     /gene="hisS"
                     /locus_tag="PK288_10840"
     CDS             863..2233
                     /gene="hisS"
                     /locus_tag="PK288_10840"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPM19466.1"
                     /translation="MKPSIPKGTRDFSPEEILKRDLIFDTVREIFRLYGYQPVETPAI
                     ENLSTLLGKYGEEGDRLIFRILNSGDFLSGIDRNDILEGGSAKLSSLICEKGLRYDLT
                     VPFARYVVQHRDEIVFPFRRYQIQPVWRADRPQKGRYREFFQCDADVIGSDSLLNEYE
                     LIRIIDDVFTRLNVNIIIRINNRKVLSGIAEYIGESAKITDITIAIDKLDKIGPEEVN
                     KELAGRGIPADAIRRLRPILELSGNTQAKLASLREILRGSETGLRGIEELATLFSYIS
                     GSPVINGTELDLTLARGLNYYTGTIMEVRAKDVSIGSICGGGRYDNLTGVFGLQGVSG
                     VGISFGADRIYDVMTQLSLFGKTASSVTQVLILNFGEKESGFAIKLRDLLKGVNCRTE
                     FYPDPVKIKKQMSYADARKIPYIVIAGDEEIKTGQVICRKMETGEQKFLSPDGLVSYI
                     RSELGC"
     gene            2323..2502
                     /locus_tag="PK288_10845"
     CDS             2323..2502
                     /locus_tag="PK288_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPM19467.1"
                     /translation="MTRRKSSENNIRALRRLSGGSSYGITIPREFLTVLKWRLRQKLE
                     LKLYRDRIVIRDWKP"
     gene            2614..3711
                     /gene="ychF"
                     /locus_tag="PK288_10850"
     CDS             2614..3711
                     /gene="ychF"
                     /locus_tag="PK288_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPM19468.1"
                     /translation="MALQCGIIGITNVGKTTIFNCISSTKGETNAFAFSATKSNMGLI
                     QVPDPRLYELRKHQVTERIVHTTIEIVDIPGLARGSNKGEGVGNRFLADIRNTDALIH
                     VLRCFDNDSLPHIDGSVDPVRDMETIDFELQVKDMESVEKKLQRLEKLVRAGDKEARH
                     GCEVLEKMKDHIGNFNNARTLELSPGDRKHVDDLFLLTMKPVMFVCNVDEKSAVAGNK
                     YVDQVRDALKGRDIEVIVIAGEIEAEIAEFENPDDRLAFLQDAGLDEPGVDRMVRAAY
                     RMLDLQTFFTVGPKEIRAWTIKRGMTAPQASGVIHSDLERGFIRAEVMKYHDFVTLGS
                     EHACREAGRFFVEGKNYVVEDGDILHIRFNV"
     gene            3721..>4737
                     /locus_tag="PK288_10855"
     CDS             3721..>4737
                     /locus_tag="PK288_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPM19469.1"
                     /translation="MRCRLFILILVVQYIALSSLSFGGTGDDKLRKIVREYGQAEVVI
                     QRPDPGTVDYLTRNVSIRSVDDKSMRIILSPATVEWFISSGFSYLLVVPSPVKGIASA
                     SSMRKAMEWDSYPTYRQYDSIMRFFAREYPSICMLDTIGTTLEGRLVMALKISDNCRM
                     TEDEPKVFLSSSMHGDETGGFILMLRLADYLLGNYDSDARTKYLVDNIELWINPLANP
                     DGTYVNGEEIYMPVRGNAAGVDLNRNFPDPELKGTVRQKETLDMMKFMSEHRFVLSAN
                     FHSGAELVNYPWDKWPRDHADKQWFYNLCRAYADTVHFYSPADYMTYLDNGVTNGYRW
                     YPVYG"
BASE COUNT         1302 a         1015 c         1198 g         1222 t
ORIGIN      
        1 aaaagtcacc caatacactt attttgcatc ttgtgggtcc cccggaaaat gacaatgccg
       61 gttactgttt taagatgctt atcggaacgg atgattcgga gatgtattat tatcaccagc
      121 agcttattga caaggctaac cccaggggcc ttttgccggc cgatcttaaa cggatgggaa
      181 aaacgtgaaa atgcagcatt gccggaaaga tgtcgtcttt aagataaaaa gcaattgtca
      241 tgaaagagat cagggagtta tcggaatcag aaaggaactg ggccatgttc tgtcaccttt
      301 cagcttatgc cggattcttt tttcctttcg gagggataat cggtccgctg atttgctggt
      361 tatcacggag ggatgagtca caatggatca accagaatgg aaaaagatca cttaatttcc
      421 agttgtcggt tcttctgtat acagttctgg ccctgccgct ctgtatcatt attgtaggca
      481 ttcccatcat catggctcta tggctcctga aaatcatctg cattgccatt gccagcgtaa
      541 aggcttcaaa aggagaagat ttcaggtatc ctcttgccat accatttatt cagtgatctt
      601 actttcggat gttttctcag gcatcggaaa gtaagcacat ttacattcgt gtcacctgga
      661 tgttcaggac cctgcattgt gcctgcaagg ctctatgctt cagagttgct tcctgacggg
      721 agattggctg aacaggaaag cgctggcgga acataccgcc ggccggaaag gatcatccgg
      781 gctgaagtct gtttatcttg catcccctct tttccctttc cccttatatt tatacttttg
      841 tctttttacc tttagcattt aaatgaagcc ttcaatacca aaagggaccc gcgatttttc
      901 tcctgaagag attctgaaaa gagacctgat ctttgatact gtaagggaga tattcaggct
      961 ttacggctat cagcctgtgg aaacaccggc gattgaaaac ctttcaaccc tgcttgggaa
     1021 atatggcgag gagggcgaca ggctcatatt caggatactt aattcaggtg attttctgtc
     1081 ggggatcgac aggaacgata tactcgaagg cggatctgca aaattgtctt cactgatctg
     1141 tgaaaaggga ttaagatacg atcttacagt acctttcgcc cgatatgtgg ttcagcaccg
     1201 ggatgaaatt gttttccctt tcaggcgcta tcagatccag ccggtatgga gggccgaccg
     1261 tccccagaag gggagatacc gcgaattctt ccagtgcgat gcggatgtaa taggaagcga
     1321 ttcgctgctt aatgagtatg agctgatcag gattatagac gacgtgttca cccggttgaa
     1381 tgttaatata atcatcagga tcaacaacag gaaagtgctt tccggaatag ccgaatatat
     1441 tggtgaatct gcaaaaataa ccgacattac gattgccatt gacaagcttg acaaaatagg
     1501 cccggaggag gttaacaagg aacttgcagg cagggggatc ccggcagatg caatccgcag
     1561 gcttcgcccc atacttgagc tgtcgggcaa tacccaggcg aaactggcca gcctccggga
     1621 aattcttcgc ggatctgaaa cagggctcag gggcattgag gaacttgcca ccctgttctc
     1681 ctatatttcc ggttcgcctg tgataaatgg caccgaactt gatctgactc ttgcccgcgg
     1741 actgaactac tacaccggta caataatgga agtaagggca aaagatgtga gtataggcag
     1801 tatctgcggc ggtggcagat atgacaatct aaccggggtt ttcggacttc agggcgtatc
     1861 aggcgtcgga atctcatttg gcgccgacag gatctatgat gtaatgactc agctctcact
     1921 tttcggaaaa accgcatcat cagtaacaca ggtacttatt ctcaatttcg gggaaaagga
     1981 atcaggattt gccataaaac tcagggactt actgaaaggt gttaactgca gaacggaatt
     2041 ctatcctgat cctgtgaaga taaagaagca gatgtcgtac gccgatgcaa gaaagatccc
     2101 ttacattgta attgcaggtg atgaagagat aaaaaccggg caggtcatct gccgaaagat
     2161 ggaaacggga gaacagaaat tcctttctcc cgacggactt gtctcttaca tccgcagcga
     2221 gctcggatgc taggtccggg cctgtgctgc tttgatgtgg taattgaaaa gactccttaa
     2281 aagactcgtt ataagacttc tttttcttaa cctgataata ttatgactcg cagaaagagc
     2341 agtgaaaaca atataagggc cctgagaaga ttatcgggcg gatccagcta cgggatcaca
     2401 atacccaggg aatttttaac agtgctgaaa tggaggctca ggcagaaact tgaactgaag
     2461 ctttacaggg acaggatcgt catccgcgac tggaagcctt agccgccggc agactgccgt
     2521 tgagcttcat gcccaaaaaa taaagactat cccccggatt agggatagaa cgggaaaatc
     2581 attaatttgc acatgaatca attatgacat ctgatggcac ttcaatgcgg aatcattgga
     2641 ataaccaatg tgggtaagac cacgatcttc aattgtattt ccagtacgaa aggtgaaacc
     2701 aatgcctttg ccttcagcgc cacaaaatca aatatggggc tgatacaggt gccggatcca
     2761 cgcctctatg agctcagaaa gcatcaggtg actgaaagga ttgtccacac caccattgaa
     2821 atagttgaca tacccggcct tgcacgcgga tcaaacaagg gtgagggcgt gggcaacagg
     2881 ttcctggccg atataaggaa caccgatgca ctgatacatg tactgcgttg ttttgacaat
     2941 gactcccttc cgcatatcga tggatcggtt gatccggtgc gtgacatgga gaccattgat
     3001 ttcgagctcc aggtaaagga catggaatcg gtggagaaaa agctgcagcg ccttgaaaaa
     3061 ctcgtacggg caggtgataa ggaagcccgg cacggatgcg aggttcttga gaaaatgaag
     3121 gatcacattg gcaatttcaa taatgcccgg accctggaat tgtcaccggg cgaccggaaa
     3181 catgtagatg atctttttct ccttaccatg aagcccgtaa tgtttgtatg caatgttgac
     3241 gaaaagtcgg cagttgccgg aaacaaatat gtcgatcagg taagagatgc cctgaagggc
     3301 cgggatatag aagtaattgt tattgccgga gaaatagaag cagagattgc cgaatttgaa
     3361 aaccctgatg accgtctggc ctttcttcag gatgcaggcc ttgatgagcc gggggttgac
     3421 cggatggtaa gggctgccta caggatgctg gatctgcaaa ctttttttac cgtcgggccc
     3481 aaggaaataa gggcctggac aattaagagg ggaatgacgg ctccgcaggc ttccggtgtg
     3541 attcacagcg atcttgaaag gggcttcata agggcagagg tgatgaaata tcacgatttt
     3601 gtaaccctgg gctcggagca tgcatgcagg gaagcaggca ggttttttgt ggaaggaaaa
     3661 aactatgtgg ttgaagacgg tgacatcctt catataagat tcaatgtata aatcttttct
     3721 atgagatgcc ggctttttat actgatactt gtcgttcagt atattgccct ttcttcctta
     3781 tcctttggcg gcacaggcga tgataagtta agaaaaattg taagggagta cgggcaggct
     3841 gaggttgtga tacaacgtcc cgatcccgga actgttgatt acctgacaag aaatgtttca
     3901 atccgttcgg tggatgacaa gagtatgcgg atcatccttt ccccggcaac agtggaatgg
     3961 ttcatatctt caggattcag ttacttactg gtggtaccct ctcctgtaaa gggtatcgca
     4021 agtgcttcaa gcatgaggaa agcaatggaa tgggattctt atcccaccta ccggcagtat
     4081 gattcaataa tgagattttt tgcaagggaa tatccttcga tctgtatgct tgacacaatt
     4141 gggaccacac ttgaaggaag acttgtcatg gccctcaaga tcagcgataa ctgcaggatg
     4201 actgaagatg agccaaaggt ttttctttcc tcctcgatgc atggtgatga aaccggcgga
     4261 ttcatcctga tgctcaggct tgctgactat cttctgggaa actatgattc ggatgccaga
     4321 acaaaatacc tcgtggataa cattgaacta tggatcaatc cgctggccaa tcccgacgga
     4381 acttatgtga acggagaaga aatctatatg cctgtcaggg gcaatgcagc gggtgttgac
     4441 cttaaccgga attttcctga cccggagctt aagggaacgg tccggcagaa agaaaccctt
     4501 gacatgatga agtttatgtc ggaacacagg ttcgtgctgt ctgcaaattt ccactcaggg
     4561 gctgaacttg ttaattaccc gtgggacaag tggccgaggg atcatgctga caagcaatgg
     4621 ttttataatc tgtgcagggc ttatgcagac actgtacatt tttattcccc ggctgattat
     4681 atgacttatc tcgataacgg tgtcaccaat ggctacaggt ggtatccggt ttatggt
//
LOCUS       DAOOYG010000170         4692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_21310_length_4692_cov_5.948889, whole genome shotgun sequence.
ACCESSION   DAOOYG010000170 DAOOYG010000000
VERSION     DAOOYG010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21310_length_4692_cov_5.948889"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PK288_10860"
     CDS             complement(<1..811)
                     /locus_tag="PK288_10860"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPM19470.1"
                     /translation="MNKGTAMEDNTDKKIPYSVSPGLSISDYDYSLPEDRIAQYPVKE
                     RDCSRLLVYRDGKISSGVFRNIVNFLPAGSLLVFNNTRVIRARLLFRKESGALIEILC
                     LEPLIPESYELSFSSRNHVEWKCIIGNLKKWKKGNITLSFVRQGKSHVLTAEKLDSLG
                     DAWRVRFSWDAGDLSFGEVIESAGHIPLPPYIRRHDEESDNKDYQTVYSRVKGSVAAP
                     TAGLHFSEKVLESLSSNNINKTELTLHVGAGTFRPVNSDRLDDHEMHCENFF"
     gene            909..1319
                     /gene="rnpB"
                     /locus_tag="PK288_10865"
     ncRNA           909..1319
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK288_10865"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1318..2019)
                     /locus_tag="PK288_10870"
     CDS             complement(1318..2019)
                     /locus_tag="PK288_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19471.1"
                     /translation="MKRLLLTYVLLTAVTLLSAQSIEEIVKNFAIANKFDKISGYQTI
                     KITGKATMMGMEMPIVLYMKNPNKLRSVATIGGQEMIQVFDGEKGWVINPMTGSRTPV
                     ELGQEEISQMMRSSVFQNYLDNYLRNNMLKLEGTENVNGRPVFKIKATIEGGNVIRFF
                     IDRNNYQLVKTVADVKQGGVPVSLESFFTDYTETNGFILPMTTTTSVAGMEFVYKYTD
                     VKVNEPMDDSIFRLK"
     gene            complement(2057..2863)
                     /gene="kdsA"
                     /locus_tag="PK288_10875"
     CDS             complement(2057..2863)
                     /gene="kdsA"
                     /locus_tag="PK288_10875"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPM19472.1"
                     /translation="MPLLKHGDSGNFLLIAGPCVVEGKEIVMRIADHLVNLSVKYRIP
                     FVFKSSYRKANRSRIDSFSGIGDIRALEILAEVRHRFSVPVITDIHNPEEAAMAAEYV
                     DILQIPAFLCRQTDLLTAAARTGKWINIKKGQFLSGSSMKHAVDKVKDSGNERIMLTD
                     RGNMFGYHDLVVDFRNIPVMKALGVPVIMDITHSLQQPNQESGVSGGMPEMIETMGKA
                     AISVGADGIFIETHPDPANARSDGANMLPMNKMEDLLIKLTALRNTVNNF"
     gene            complement(3054..4352)
                     /locus_tag="PK288_10880"
     CDS             complement(3054..4352)
                     /locus_tag="PK288_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPM19473.1"
                     /translation="MLANQPLAERLRPRDLDDFCGQDHLLGKNAVLRRLIDSGNIPSL
                     ILWGPPGVGKTTLATIIANNLKRPFFHLSAVHSGVKDVRETIDKARKQQFFSQPNPIL
                     FIDEIHRFSKSQQDSLLGAVEQGIITLIGATTENPSFEVISPLLSRCQVYVMNPLSES
                     ELTSLLNRALKKDHYLSGYDIEIRENEALLRISGGDARKLFNALELVVSSETNETRSE
                     KIIIDNEKVLRHVQRNIALYDKNGEQHYDIISAFIKSMRGSDPNASVYWLARMVEGGE
                     DPKFIARRMLILAAEDIGLANPNALLLAQACFDAVNVIGWPESRIILSETAIYLASSP
                     KSNASYMAIEKATEAVNEKGDLPVPLHLRNAPTKLMKDLGYHKDYKYAHAYEGNFVRD
                     NYLPEGLEGTRFYDPGSSPKEAEIRKRLENLWKDTYDYDK"
     gene            4501..>4692
                     /locus_tag="PK288_10885"
     CDS             4501..>4692
                     /locus_tag="PK288_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19474.1"
                     /translation="MMIKRIIPLFSLVLFWGCKGSSTPQVLINREGRNIIADSGMVVS
                     AHSESSRIGVEILRKGGNAV"
BASE COUNT         1167 a         1100 c         1035 g         1390 t
ORIGIN      
        1 caaaaaagtt ttcgcaatgc atttcatgat catcaagcct gtcagaattt acgggcctga
       61 aagttccagc ccccacatga agggtaagct ctgtcttatt aatattatta gacgacagac
      121 tttcaagtac tttttcggag aaatgcaggc ctgctgtcgg tgctgcgact gatcccttta
      181 cccgcgaata cactgtctgg tagtccttgt tgtcgctttc ctcgtcatgc cttcttatat
      241 agggagggag gggaatatgt ccggcggatt cgattacttc cccgaaactc aagtcacctg
      301 catcccagga aaacctgact ctccatgcat ctcccagtga atctaatttt tctgcggtca
      361 acacgtggct ttttccctga cggacaaatg aaagggttat attgcccttc ttccatttct
      421 tcagatttcc tatgatacat ttccattcga catgattcct tgaactgaac gagagttcat
      481 agctctccgg gattaatggt tcaaggcaca gtatttcaat aagcgccccg ctttccttcc
      541 tgaataaaag cctggccctt ataacacggg tattgttgaa taccagcagg ctgccggcag
      601 gcagaaaatt cacgatattg cggaaaacac cggatgatat tttaccgtcc ctgtaaacaa
      661 gcagccttga acagtctctt tccttcaccg gatactgtgc aattctatct tcgggcaatg
      721 agtaatcata gtcgcttatt gacagtcccg gactaacaga gtaaggtatc tttttgtccg
      781 tattatcttc catcgctgtt cctttattca tttgcacctg tcaactactg ccatgctggt
      841 ttttccgggc tcaaaattaa gcaaatatca tatttgttct atgtaaggga gtttattaac
      901 tttgcagtac agtctgccgt tccatcgttc cccgaatccg gttcatgccg tttaggcggg
      961 gaaaggaaag tccgggcaac acagagcacc atactttcta acagaaagat atccgtgagg
     1021 atatatcagg tgtaacagaa aataaccacc cggggttaag gtgacaaccg aaataccggg
     1081 aacgggtgaa aaggtgaggt aaaggctcac cggtattgat ggtgacatca atagccgtac
     1141 accttatggg ttgaaagacc atgtataccg gccataagga ttgctcgtcc gatgccgggg
     1201 ggtgggtcgt acgatccggt cagcaatggc cggaccagat aaatgatgga atccggttaa
     1261 ataatctttt gcagatagtc tgaccggcac agaatccggc ttacaggcag gctgttttta
     1321 tttcagtctg aatattgaat catccatcgg ctcattcacc ttcacatccg tgtatttgta
     1381 aacaaactcc atgccggcta cagatgtggt tgtggtcatg ggaagaataa aaccattcgt
     1441 ttcagtatag tcggtgaaaa atgattcgag cgaaaccgga acgcctccct gtttaacatc
     1501 tgctacggtt ttgacaagct ggtaattgtt cctgtcaata aaaaacctga tgacatttcc
     1561 tccttctatg gtggctttga ttttgaaaac cggcctgcca ttgacatttt cagttccttc
     1621 aagtttcagc atgttgtttc tcagataatt atcgaggtaa ttctgaaaaa cgctgcttct
     1681 catcatttgg ctgatctcct cctgtccaag ctccacaggt gttctggaac ctgtcatcgg
     1741 attgataacc cagccttttt caccgtcaaa aacctggatc atctcctggc cgcctattgt
     1801 tgcaacggat ctcagcttat tcggattttt catataaagg actatcggca tttccatccc
     1861 catcattgtc gcctttcccg taatctttat agtctgatag ccggagatct tatcgaattt
     1921 gttggcgatg gcgaagtttt tcacaatttc ttcgatcgac tgggctgaaa gcagggtgac
     1981 agcagtgagc aggacatagg tcagaagcag tcttttcatt tgcagttagt atttgtgttt
     2041 aaaaattatt ttgtgtttaa aaattattta cggtattcct gagggctgta agtttgatga
     2101 gcagatcttc cattttattc attggaagca tgttggcgcc atcagatctg gcattggcgg
     2161 gatcggggtg ggtttctata aaaattccgt cggctcccac gctaatggca gcctttccca
     2221 tggtttcgat catttccggc atgcctcccg aaacacccga ttcctgattc ggctgctgga
     2281 gcgaatgagt gatgtccatt ataaccggta caccgagtgc cttcatgaca ggaatattcc
     2341 tgaaatcaac cacgaggtcg tggtagccga acatgttgcc ccggtcggtt agcatgatcc
     2401 tttcatttcc cgaatccttt accttgtcaa ccgcatgctt cattgaagac cctgaaagga
     2461 actgtccttt ctttatattt atccattttc ccgttcttgc cgccgctgtc agaagatcag
     2521 tctgccggca cagaaacgca ggtatctgca ggatgtcaac atattctgct gccatcgcgg
     2581 cttcttcagg attatgaatg tcggttatta ccggcaccga gaacctgtgc cttacttccg
     2641 caagtatttc cagtgccctg atgtcgccga tgcctgaaaa ggaatcgatc ctcgacctgt
     2701 tcgctttcct gtacgaagat ttgaaaacaa aggggatcct gtatttaact gacaaattca
     2761 caaggtgatc tgcaatccgc attacaattt cttttccttc cactacacac ggtcccgcga
     2821 tcaggaggaa attgcctgaa tcaccatgtt tcagcagcgg gatatcaata ccggttttca
     2881 tccttgcata tttttttttc aggatttcct gaaacgccaa aggtaataaa attaaactct
     2941 gaaattttaa atctgctgat tgcagatata tgttttatcg atgtgacggt tctgatttat
     3001 tcacccggag gcagtatact gaaataaagg aaaaatccca gaaggcctga ttttcatttg
     3061 tcataatcat aggtgtcctt ccataaattc tcaagccgtt ttcttatttc cgcctccttt
     3121 ggtgatgatc cgggatcgta aaacctggtt ccttccagcc cctcggggag atagttatcc
     3181 ctgacaaagt tgccttcgta ggcatgagcg tatttgtaat ccttatgata accgagatcc
     3241 ttcatgagct ttgtaggtgc atttctcaga tgcaatggaa ccggcagatc tcccttttcg
     3301 tttacagcct ccgtagcttt ttcaattgcc atgtatgatg cattgctttt gggagatgat
     3361 gcaaggtaaa tggctgtttc agacaggatg atcctcgatt cgggccatcc tatgacattt
     3421 acggcatcaa agcatgcctg ggcaagcagc agcgcgttgg gattggcaag gcctatgtct
     3481 tcggctgcca gtatcagcat ccgtcttgct atgaatttcg gatcctcacc tccctcaacc
     3541 atccttgcga gccagtaaac cgaagcattc gggtcactgc ccctcatcga ttttataaat
     3601 gctgatatta tatcgtaatg ctgctctccg ttcttgtcat agagggctat attcctctgg
     3661 acatgacgaa gtaccttctc gttgtcaatt atgatttttt ccgacctggt ttcattggtt
     3721 tcggatgaca ctaccagttc aagggcattg aacagcttcc tggcatcacc gccagaaatc
     3781 ctcagcaaag cttcattttc cctgatttca atgtcatatc ctgaaagata atgatctttt
     3841 ttgagtgccc tgttcagaag cgaggtcagt tcggattcgc tgagggggtt cataacatag
     3901 acctggcacc gcgaaagcaa cggagaaata acttcaaagg aggggttctc agttgttgca
     3961 cctatcaggg ttattattcc ctgttccact gcccccagaa gcgaatcctg ctgagacttg
     4021 ctgaaacgat ggatctcatc tataaataaa atcggattgg gttgactgaa aaactgctgc
     4081 ttcctggcct tatcaatggt ttctctcaca tccttaacgc cggaatgtac tgcactcagg
     4141 tgaaagaagg gacgtttcag gttgttcgca ataattgtgg caagggttgt ttttcctaca
     4201 cccggggggc cccagaggat cagggagggt atatttcccg aatcaatcag cctccggagc
     4261 acggcatttt tgcccagcag atgatcctgt ccgcagaaat catccaggtc ccttggccgc
     4321 agcctttcgg caagaggttg atttgccagc attactgttc tttatctgcc caaaattagt
     4381 aaaaacaacg aacagatgaa tcaacaatat tcccggtcat acattggcgg atttattttg
     4441 ctatttttgc atgcctgcac ctgatattgg atttcagcta tcaggattta ttttaagtac
     4501 atgatgatca agagaataat tccgttgttt tccctggtcc ttttctgggg atgcaagggc
     4561 agtagcaccc cgcaggttct gattaaccgg gaaggccgga atatcatcgc cgacagcgga
     4621 atggtagtgt ccgcgcattc agaaagttcg cggatagggg ttgaaatact ccggaagggt
     4681 ggaaatgcgg tt
//
LOCUS       DAOOYG010000171         4686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_21344_length_4686_cov_7.794429, whole genome shotgun sequence.
ACCESSION   DAOOYG010000171 DAOOYG010000000
VERSION     DAOOYG010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21344_length_4686_cov_7.794429"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /locus_tag="PK288_10890"
     CDS             <1..1773
                     /locus_tag="PK288_10890"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPM19475.1"
                     /translation="KSITFQVPALAMEGTCLVITPLVALMKEQVTRLISMDIKAMAIH
                     SGMSREEIDISLENCIYGDYKFLYVSPERIATRSFQEKAARLNLSLVAVDEAHCISQW
                     GYDFRPSYLKIASLRSLISDKVPFLALTASATAQVIEDILDKLEFRERNVLKTSFSRK
                     NISYMVRNVEDKGVYLVKTLKKARGSGIIYVRSRKRSREIAMLLAENGISADFYHAGL
                     SSELRDKKQSSWSSGETRIIVATNAFGMGIDKPDVRFVIHWDIPDSVESYFQESGRAG
                     RDNKPSFAVLLYSPSDKNRLNDSLRQKFPPVEKIKDTYEALCNYLQLPLGAGKNSVFD
                     FNMSEFVSRFRLPVIETYNSLSFLQREGYVEFTEEINNPSRVHFIVGRDDLYKFQVAN
                     ESFDGFIKLLLRSYTGMFSGFVAISEEALAGKSGLSRDTVYQYLVKLSALNIIRYIPG
                     KTTALVIFTEERLERKALMISPGNYLYVKEKYVARMNRMIDYAESRNRCRAVILLDYF
                     GEVCDRCGICDVCRERNELELSKYEFDLIKEGIKDILSREKPDAGMLVKMVDHPEDKV
                     VRVLRWLLDHNKITRDNDNRLSWI"
     gene            complement(1852..2307)
                     /locus_tag="PK288_10895"
     CDS             complement(1852..2307)
                     /locus_tag="PK288_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPM19476.1"
                     /translation="MKKHILTIGILIIGLVSCRSNNPISSSDSAGQEKEILAALEKFK
                     TGIIDADRAVLEDIFADKLVYGHSGGKVQNKAECIDEIVSLQPNDYLTIDMTDQTITI
                     SGETAIVRHIYSSEFTSNGIPGSLRIGNVLVWQKQDGKWKLLARQAYKL"
     gene            complement(2361..3284)
                     /locus_tag="PK288_10900"
     CDS             complement(2361..3284)
                     /locus_tag="PK288_10900"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19477.1"
                     /translation="MKTKLLLSSLLIVIYLQSAISQTISKDELIFLTSEWKGERFPDG
                     RPKISDDLIKRARNIGIEEAWQILSNNGYVNQYEGNWKTSNGYTPVVGRAVTATFIPS
                     RPDLDKNITARGHKSGFNGASNSWPISVLQKGDVYVADCFGKINEGTLIGDNLGNAIF
                     NKTGNGVIFDGAARDMSGLSEIDGFNAYVRDFHPSFLKNVILMGLNTPTRIGGAVVLP
                     GDLVMTEGGGVLFIPAHMAEKVILTAEFISIRDKFSHERLKQGRYNAGQIDSQWTNEI
                     IEDFMKWIGQHPEVPQLTRSQVDEFMKQRTW"
     gene            complement(3526..>4686)
                     /locus_tag="PK288_10905"
     CDS             complement(3526..>4686)
                     /locus_tag="PK288_10905"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HPM19478.1"
                     /translation="LYMNQRLWGTTTKSWEVENAASYAVRNENSEINTHIYNIFTNRP
                     TASMCLATDFWKDKYASLCDSAINTYMAGGIYMDQACLALTCYARDHGHPAGPGKYWA
                     ENFSILSGKIRNKINRDKQCILAGEGAGEAWLPYLDLFLTLAVSRERYSGIDNWQVIP
                     FFQAVYHQYAITYGNYSSLLVPPYDELWPGEFAPADPLEKLHDDFNRQFLMEQARSFA
                     WGLQPTISNYQKFLASDRKEEIGYVLNLAKVRNRGLKYLLYGKFLRSPEINIPVAGFD
                     ISRLSIYAGKEGKSITRFHGEFPLIYTGTWQAADKSIGIAVASISDEPFNISFETDAG
                     YYGIEPSGKVNIIDRQGIRLLKQYSDGQVKIEYKLKPKDICIIEIEPAKNNF"
BASE COUNT         1191 a         1015 c         1118 g         1362 t
ORIGIN      
        1 aaatcaataa ccttccaggt accagccctg gccatggaag ggacctgcct ggttataaca
       61 cctctggttg ccctgatgaa ggaacaggtc accaggctta tcagcatgga tattaaagct
      121 atggcaattc actccgggat gtcgagggaa gaaatagata tctccctgga aaactgcatt
      181 tacggagact ataaattcct ttatgtctct cccgaaagaa tagctacccg ttctttccag
      241 gaaaaggctg cgcgcctgaa cctgtcgctg gtagctgttg acgaggcaca ttgcatttcg
      301 cagtggggtt atgatttcag gccgtcatat ctcaaaattg catccttgcg gagcctgata
      361 tcagacaagg taccctttct tgcacttaca gcatcggcaa cggcgcaggt gatagaggat
      421 attctggata agctggaatt cagggagaga aatgtcctga aaaccagctt ctccaggaaa
      481 aatatttcct atatggtcag gaacgtggaa gataagggtg tttacctggt aaaaaccctg
      541 aaaaaggcca ggggaagcgg gataatctat gttagaagca gaaagaggag cagggaaata
      601 gccatgctcc tggccgaaaa tggaataagt gccgactttt atcatgcggg cttgagttct
      661 gaacttcgcg ataaaaagca aagctcctgg tcatctggtg aaaccaggat cattgtggca
      721 acaaatgcat tcggaatggg aattgacaaa cccgatgtca gatttgtaat acattgggat
      781 attccggatt cggtcgagag ttattttcag gaaagcggaa gagccggccg tgacaacaaa
      841 ccatcatttg cagttctgct ttattcaccg tcagataaga acaggctcaa tgactcactg
      901 cgtcagaaat ttcctcctgt tgaaaaaata aaggacacct acgaggcatt gtgcaattat
      961 ctgcagctgc cactgggagc tggtaagaac agtgtgtttg acttcaatat gtctgaattt
     1021 gtttcacgtt tcaggttgcc cgtgattgaa acctataaca gcctttcttt cctgcagaga
     1081 gagggatatg tggaatttac cgaagaaata aacaatcctt cgagggtgca ttttatcgtg
     1141 ggcagggacg atctttacaa attccaggtg gcaaatgaaa gttttgacgg gttcattaaa
     1201 cttctgctaa gatcatacac cggaatgttt tcgggttttg ttgcaataag cgaggaggcg
     1261 ctggcaggaa aatcaggatt aagccgggat accgtatatc agtatctcgt gaagctttcg
     1321 gcgctgaaca ttataaggta cattccgggc aaaactactg cacttgtgat ctttaccgag
     1381 gaaaggctcg aaagaaaggc actgatgatc tctcccggca actatcttta cgttaaggag
     1441 aagtatgtcg caaggatgaa cagaatgatt gattatgccg aatccaggaa cagatgcagg
     1501 gctgttatcc ttcttgatta ttttggagaa gtttgtgaca ggtgcggcat ttgcgatgtg
     1561 tgccgcgaaa gaaatgaact tgaactgagc aagtatgaat ttgatctgat aaaggaaggg
     1621 ataaaggata ttctgtcaag ggaaaagccc gatgccggaa tgcttgtgaa aatggttgat
     1681 catcctgagg acaaggttgt gagggtttta cgctggcttc ttgatcataa caagataacc
     1741 cgtgacaatg acaacaggct ttcctggatc tgatgccaaa tcactcaggc tggcagggac
     1801 agggatttac cctgccggaa tgaagtacaa accgggcagg gtaaataatt tctatagttt
     1861 atatgcctgt cgtgcaagaa gtttccattt gccgtcctgt ttctgccaga ccagaacatt
     1921 tccgattctg agactgccgg gaataccgtt gctggtaaat tcagatgaat agatatggcg
     1981 tacaattgcc gtttcacccg aaatcgtgat tgtctgatcg gtcatgtcga tcgtcaggta
     2041 gtcgtttggc tgaaggctga cgatttcgtc aatgcattct gccttgtttt gaacctttcc
     2101 gcctgaatga ccgtaaacca gtttgtctgc aaaaatgtct tccagaacag cccggtcagc
     2161 atctattatt ccggttttga atttttccag tgcagccagt atttcctttt cctgtccggc
     2221 agaatctgat gaggaaattg gattgtttga tctgcaggag actaatccta tgatgagaat
     2281 gccaattgtt agaatatgtt ttttcatttt tgattgtttt gctttttcct cttgttttcg
     2341 gtagtttgga atgccgtgaa ctaccatgtt ctttgtttca tgaactcatc tacctggctt
     2401 cttgtaagct gcgggacttc cggatgctgt ccgatccatt tcatgaaatc ctcaatgatt
     2461 tcattggtcc actgactgtc aatctggccg gcattgtagc gtccctgttt cagtctttca
     2521 tggctgaatt tgtcgcgtat tgatataaat tcagcagtaa ggataacttt ttcagccata
     2581 tgggccggga tgaaaagcac gccgccgcct tccgtcatta caaggtcacc cggaaggacc
     2641 acggcaccgc caatgcgggt aggagtattc agccccatca ggataacgtt tttcagaaat
     2701 gaaggatgaa aatccctgac ataggcattg aatccgtcaa tttcagacag tcccgacatg
     2761 tccctggcag caccgtcgaa aattaccccg ttcccggttt tgttgaaaat tgcattgccc
     2821 aggttgtcac ctataagcgt gccttcattg atcttgccga agcaatcagc cacgtaaaca
     2881 tcgcctttct gaagcaccga gatcggccat gaattacttg ccccgttaaa acctgatttg
     2941 tgccccctgg ccgtaatgtt cttgtccaga tcggggcggc tgggaataaa tgtggcagta
     3001 actgcacgac ctacaaccgg ggtgtagcca ttggaggtct tccagttacc ttcatactgg
     3061 tttacgtatc cgttattgct gagaatctgc cacgcctcct cgatacctat attcctggcc
     3121 cttttgatca ggtcatcgga aatcttcggt ctcccgtcgg gaaaacgttc acctttccat
     3181 tcagaggtga ggaaaatcag ttcgtccttg gagattgtct gactgatagc tgactggagg
     3241 tatatcacaa tcagaaggga ggagagtaaa agctttgttt tcattataat atgattttga
     3301 tttttatttt tatgttacga cggctgcctg ctcccgggca tggaatccgt atgatggaaa
     3361 tcaatttcac acttgtttgt gaatgacatt catgtgtggt tcattgtatg ataattttaa
     3421 taaggatttc aattgttgct ggctaaaata cgcaaagaag gcttaccata caacatttta
     3481 aattttttta gctatctggt tcgtccggga attgggtcag atctttcaaa agttattttt
     3541 ggccggttct atttctatga tacatatgtc tttaggtttc agtttgtatt cgatttttac
     3601 ttgtccgtca gaatactgtt tcaacagtcg tattccctgc cggtcaataa tgttaacctt
     3661 tcccgatggt tcaataccat aatatccggc atctgtttca aatgagatgt taaatggttc
     3721 atcgcttata cttgcgacag ctatcccgat gcttttatca gctgcctgcc aggttccggt
     3781 gtatataagc gggaattctc catggaacct tgttatgctt ttgccttcct ttcccgcgta
     3841 gattgagagc cgggagatat cgaacccggc aactggtatg tttatttcag gactcctcag
     3901 gaattttccg tacagcaggt atttcaggcc ccggttcctt actttggcaa gattcagcac
     3961 ataaccgatt tcttcctttc tatcgctggc caggaatttc tgatagtttg aaatggtggg
     4021 ttgcaatccc catgcgaatg accttgcctg ttccattaga aactgccggt taaagtcatc
     4081 atggagtttc tccagcggat cggccggtgc aaattccccc ggccatagtt catcgtatgg
     4141 cggaacaagc aatgatgagt agttcccgta ggtaatggca tactggtgat atactgcctg
     4201 aaaaaacggg ataacctgcc agttgtcaat accggaatat ctttccctgc ttaccgccag
     4261 ggtaaggaaa agatcgaggt atggcagcca tgcttctccg gcgccttctc cggcaaggat
     4321 gcattgtttg tcccggttta ttttgttgcg gatcttcccg gagaggatgc tgaaattctc
     4381 ggcccagtat ttcccggggc ccgccggatg gccatgatcc cgggcatagc aggtaagggc
     4441 cagacatgcc tgatccatat atattccgcc ggccatatag gtgtttattg cactgtcgca
     4501 cagtgaagcg tatttgtcct tccagaagtc ggtggcaagg cacatggaag ctgtgggcct
     4561 gttggtgaaa atattgtata tgtgtgtgtt tatttcactg ttctcgttcc ttacggcata
     4621 agatgctgca ttttccacct cccagctttt ggttgtcgtg ccccacaggc gctgattcat
     4681 atacaa
//
LOCUS       DAOOYG010000172         4681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_21372_length_4681_cov_8.593385, whole genome shotgun sequence.
ACCESSION   DAOOYG010000172 DAOOYG010000000
VERSION     DAOOYG010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21372_length_4681_cov_8.593385"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1928
                     /gene="ppdK"
                     /locus_tag="PK288_10910"
     CDS             <1..1928
                     /gene="ppdK"
                     /locus_tag="PK288_10910"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPM19479.1"
                     /translation="ATGVAFTRNPATGENLFYGEWLVNAQGEDVVAGIRTPSPLNNET
                     KNEQNKHLKSMQEAMPGTYKELCDIRDILEKSFHDMLDIEFTIQEGVLYMLQCRAGKR
                     TGTAALNMAMDMLHEKLIDEKTAVLRVDPAQLDELLHPICDPKAESKVKVLVKGLPAG
                     PGAAVGKVVFTAEDAVAWFRKGEKVILLREETNPEDVEGMRAAEGILTARGGMTSHAA
                     LVARGWGKCCIVGASALHVDVAAKTAKISGSSIVIKEGDVLTLNGTKGAVYTGALTLM
                     DASENPRFREFMALVDKYRKMGVRTNADTPADAKIARDFGAEGIGLFRTEHMFYGKGS
                     EEPLFLLRKMILSATEAERRKALDELYSYSKENMKATLDAMDGLAVTFRLLDPPLHEF
                     VPQGAEKQEELAKALGIRKEDVAKRGEALHESNPMMGHRGVRLGVTYPEITEMQLRSM
                     FEATAELIKEGKHPKPELMVPVTCDVSELDFTKKIFDNIYNEVCKKYGLSKIDCKYGT
                     MIEIPRACLLADRMAKTAEFFSFGTNDLSQMTFGFSRDDIGGFMNAYLDNKMLAADPF
                     QTIDQDGVGQLINMAVQKGRATRSDLKIGICGEQGGDPASVEFCFKAGLTYVSCSPFR
                     VPIARLAAAQASIKSGK"
     gene            2206..3996
                     /locus_tag="PK288_10915"
     CDS             2206..3996
                     /locus_tag="PK288_10915"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPM19480.1"
                     /translation="MTNNRKLIVWVKEWAEICQPEKIHWCDGSDKENRMLLDMMVNNG
                     SAIKLNEDKRPGSYYFQSDPSDVARVENRTFICSEKQEDAGPSNNWCDPREMKNTLLG
                     LYKGCMKGRTMYIIPFSMGPLGSEIAHIGVEITDSPYVVVNMRIMTRMGKAVLDVLGD
                     KDFVPCIHSVGAPLKPGEKDKKWPCAPIEQKFITHFPETNEIWSYGSGYGGNALLGKK
                     CFALRIASNMARREGWLAEHMLIMGITNPAGIKKYFAAAFPSACGKTNLAMLIPTIPG
                     WKVECVGDDIAWMKFGKDGRLYAINPEYGFFGVAPYTSMKTNPNAMLSCRANTIFTNT
                     ALTPDGDIWWEGIGHDIPENTITWTDEKYDPANGKPAAHPNARFTAPAGQCPVIDSDW
                     ENPAGVPISAILFGGRRPDTLPLVYQSFDWNHGVFAGSIAGSEVTAAAIGLKAGVRRD
                     PFAMLPFCGYHMGDYFSHWIRIGKNAPDKNKLPKIFNVNWFRKSSQGKFLWPGYGDNI
                     RALKWIFERIEGADNATETPVGLVPKPGSIDTSGLNEIEKDMPEILKTDKNEWLTETE
                     LITTHYATFGDRLPEELKHQFEALKARLNR"
     gene            4007..4639
                     /gene="plsY"
                     /locus_tag="PK288_10920"
     CDS             4007..4639
                     /gene="plsY"
                     /locus_tag="PK288_10920"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPM19481.1"
                     /translation="MTTIYIIAAGLTAYLLGSIPSAVWVGRLFHGIDVREHGSGNAGA
                     TNVIRVLGWKTGIPVLLFDMAKGFLAAMLPRLFNLAPQDSAMLVNLQIFTGLMAITGH
                     IFPVFAGFRGGKGVAAMFGILLAIHPPVTLLCLGVFLVFFLSTGIVSIGSMAAGIAFP
                     IFLLTFFKTPSVFFRIFSVVVAVALLVTHRKNIQRLLKGEEKRLFGKKKG"
BASE COUNT         1350 a         1000 c         1135 g         1196 t
ORIGIN      
        1 cagctactgg tgttgctttt acccgtaatc ctgccacagg tgagaacctg ttctatggcg
       61 aatggcttgt aaatgcacag ggcgaagacg tggttgcagg tatccgcacc cccagccctc
      121 ttaacaatga aacgaagaat gagcagaaca aacacctgaa aagcatgcag gaagccatgc
      181 cgggtacata caaggaatta tgtgatatcc gtgatatcct tgagaaatcc ttccacgaca
      241 tgctcgatat agaattcaca atccaggaag gagttcttta tatgcttcag tgccgtgccg
      301 gtaaaagaac cggaactgcg gctctcaata tggcaatgga tatgcttcat gaaaaactga
      361 tcgatgaaaa aacggcagta ttacgtgttg atcccgcaca gctcgatgaa cttcttcacc
      421 cgatctgtga ccccaaagcc gaatctaaag taaaagtatt agtcaagggt ctgcctgcag
      481 gccccggagc agctgtcggt aaggttgtat ttacagccga ggatgctgtt gcatggttcc
      541 gtaaaggaga gaaagttatt cttcttcgcg aagagaccaa tcctgaagac gttgaaggaa
      601 tgcgtgctgc agaaggtatc cttaccgccc gcggtggtat gacatcccac gcagcccttg
      661 ttgcccgcgg ctggggtaaa tgttgtattg tcggagccag cgctcttcat gttgatgtag
      721 ctgctaaaac agcaaaaatt tccggatcga gtattgtaat taaagagggt gatgtcctta
      781 cactcaacgg gacaaaagga gctgtatata ccggtgcatt aacccttatg gatgcttcag
      841 agaatccaag gttcagggaa ttcatggcac tggttgacaa gtatcgtaaa atgggagttc
      901 gcacgaacgc cgacactcct gccgatgcaa aaattgcacg cgacttcggt gctgaaggta
      961 tcggcctttt ccggaccgaa catatgttct atggaaaggg ttcagaagaa cctcttttcc
     1021 tgctgcgtaa gatgatatta agcgctaccg aagctgaaag acggaaagcc cttgatgagc
     1081 tttattcata ttcgaaagag aacatgaaag ccacccttga tgcaatggac gggctggctg
     1141 taactttcag gctcctcgac ccgccgcttc atgaattcgt tccacaggga gcagaaaaac
     1201 aggaagaact ggcaaaagct ctcggcatca gaaaagaaga tgtggcaaaa cgcggagaag
     1261 cattacatga atcaaatccg atgatgggtc accgcggtgt ccgcctggga gtaacctatc
     1321 ccgagattac tgaaatgcag ctcagatcaa tgttcgaagc taccgccgaa cttattaagg
     1381 aaggtaaaca cccgaaacct gaattgatgg tccccgtaac atgcgacgtt tcagaacttg
     1441 attttacaaa gaaaatcttt gacaatatct ataatgaagt atgtaaaaaa tacggcctca
     1501 gcaagataga ttgcaaatac ggaaccatga tcgagatccc gagagcttgt ctgcttgccg
     1561 accgtatggc taaaacagct gagttctttt catttggtac caatgacctt tcacagatga
     1621 cattcggatt cagccgtgat gatatcggcg gatttatgaa tgcatacctc gataataaga
     1681 tgctggctgc cgatcctttc cagacaattg accaggatgg tgttggtcag cttataaata
     1741 tggctgtcca gaagggaaga gctacaagat cagatctgaa gatcggaatc tgcggtgaac
     1801 agggtggtga ccctgcttca gttgaattct gcttcaaggc cggtctgacc tacgtaagct
     1861 gctcaccttt cagggtgccc atagccagac tggcagcggc acaggcttca atcaaatccg
     1921 gaaaataatt actgaatctg attttgttat ataaagggac gttgaatgta acgccccttt
     1981 tttaattatc tgtacagaaa atattaatca tttaagccat attggtcata attttggaat
     2041 aacaggagat tattaattaa aaccattaac aattatcagt gattcagaag aaattgttag
     2101 taaaatcata ataaaataaa attaaggttg tattaattct ggaatctatt catatatttg
     2161 gagcgatagt tgggtgataa atctttaata tttaaaagaa tttttatgac gaacaaccgg
     2221 aaattaatcg tatgggtaaa agaatgggct gaaatctgtc agcctgaaaa aatacattgg
     2281 tgcgacggtt ctgataaaga aaatcggatg ctgcttgata tgatggttaa taatggaagt
     2341 gccataaagc tcaacgaaga taaacgtccg ggaagttatt atttccagag tgatcccagt
     2401 gatgttgccc gtgtggagaa ccgtactttt atctgttcgg aaaagcagga agatgctggt
     2461 ccgtcgaaca actggtgtga tccacgcgaa atgaaaaata ccctgctagg tttatataaa
     2521 ggttgtatga aaggccgtac aatgtatatc atacccttca gcatgggccc gcttggttca
     2581 gaaatagctc acataggtgt cgagatcacc gattccccct atgttgtggt taatatgagg
     2641 atcatgacaa ggatggggaa agctgtcctg gatgttctcg gggataaaga ttttgttccc
     2701 tgtatacatt cggtcggggc gcctcttaag cctggagaaa aagataaaaa atggccatgt
     2761 gctcctatag aacagaaatt tatcactcac ttccccgaga ccaatgagat atggtcgtat
     2821 ggaagcggtt atggtggaaa tgcgcttctg gggaagaaat gctttgccct gaggattgct
     2881 tccaatatgg cacgcaggga aggttggctg gccgaacata tgctcataat gggtattaca
     2941 aatcctgccg gaatcaagaa atattttgcg gcagcattcc cgagtgcgtg cggtaaaacg
     3001 aaccttgcta tgctgatccc gacaatcccc ggatggaaag ttgaatgtgt cggagatgat
     3061 attgcctgga tgaaattcgg gaaagacggc cgcttatatg cgatcaatcc cgagtatggc
     3121 tttttcggag tggctccata cacatcaatg aagacgaatc ccaatgccat gctttcatgc
     3181 cgggcaaata caattttcac aaacactgcc cttacccccg atggtgatat atggtgggaa
     3241 ggtatcggcc acgacatacc ggaaaataca attacatgga cagatgagaa atatgatcct
     3301 gcgaacggca agccggcagc tcatccgaat gcacggttta cagctccggc cggccaatgc
     3361 cctgttattg acagcgactg ggaaaatcct gccggagttc cgatctcggc aatccttttc
     3421 ggaggtcgtc gccccgatac attaccgctg gtttaccagt ccttcgactg gaatcatggt
     3481 gtttttgcag gatcaatagc cggttccgaa gtaactgcag cagcaatagg actgaaagcc
     3541 ggtgtccgcc gtgatccttt tgcaatgctt ccgttctgcg gatatcatat gggagactat
     3601 ttctcacact ggatcaggat cggaaagaac gctcctgaca aaaacaaact gccgaaaata
     3661 tttaacgtca actggttcag gaaaagttca cagggtaagt tcctgtggcc gggatatggt
     3721 gataacatca gggcacttaa atggatattc gaacgtattg agggagccga caacgccact
     3781 gagacaccgg tcggactggt tccaaaaccc ggaagtattg atacaagcgg actgaacgaa
     3841 attgaaaaag atatgcctga gatcctcaaa actgataaaa acgaatggct gactgaaact
     3901 gaattaataa cgacgcatta tgctacattc ggcgaccgtc tgcctgaaga acttaaacat
     3961 cagtttgaag ctctgaaggc aaggttgaac aggtaaatat cctgaaatga caacaatcta
     4021 tataatcgct gccggcctga cagcgtattt acttgggtca atcccgtctg cagtatgggt
     4081 aggaaggctt tttcacggta tcgatgtcag ggaacacggc agcggaaatg cgggtgcaac
     4141 aaatgtaata cgggttctcg gctggaaaac cgggatcccg gtattgttat ttgatatggc
     4201 caagggattt ctggcagcaa tgcttccccg gctgtttaac ctggctcctc aagacagtgc
     4261 catgcttgtc aatcttcaga tctttacagg tttaatggct ataacaggtc atattttccc
     4321 tgtttttgca ggattcaggg gaggaaaagg agttgctgcc atgttcggca tcctcctggc
     4381 tatccatcct ccggtaacac tcttatgcct gggcgtcttc ctggtcttct tcctgtcaac
     4441 cggaatagta tcaataggat caatggctgc cggaatagcg tttcccatat ttttacttac
     4501 cttcttcaaa acaccctccg tttttttcag gatattttca gtagtggttg ctgttgcttt
     4561 gctggtaact cacaggaaga atatccagcg cctgttaaaa ggggaggaaa agagattatt
     4621 cgggaaaaag aaggggtgat gtcatgtatt ctcccctgtg ccccgtagcc gtcaacctaa
     4681 g
//
LOCUS       DAOOYG010000173         4653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_21556_length_4653_cov_5.636146, whole genome shotgun sequence.
ACCESSION   DAOOYG010000173 DAOOYG010000000
VERSION     DAOOYG010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21556_length_4653_cov_5.636146"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PK288_10925"
     CDS             <1..271
                     /locus_tag="PK288_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005649514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPM19482.1"
                     /translation="DYSELFPVVKQKVKAIVCLGRDNRKIIEAFTDKVPTIVEAGSME
                     EAVRSAYYLAKKGQTVLLSPACASFDLFNNYEDRGRQFKTAVRNL"
     gene            277..1494
                     /locus_tag="PK288_10930"
     CDS             277..1494
                     /locus_tag="PK288_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPM19483.1"
                     /translation="MAMQSVRLRNAFKGDRVIWALVAVFMFYSLLAVYSSSASVAYLK
                     FGGNNTYFLRSQSLMLFMSLIIIVIVHYLPYRIYSRLSGIILLGSIALLVLTFLIGAR
                     VNEAVRTIEIPGTGFRLQTSDVAKVALVIYLARALSQFNDELGNFMIVTKYMMLPIAV
                     VCGLIMPENLSTALMVFLISMIILFIGRVPLRFLLAYAGIAVAGVVLFAMVLSMVSEK
                     NRVEVWKTRIERYFSEDTNDEADYQSNQAKIAISTGGLFGKAPGKSTQRNILPQSNSD
                     FIFAIIIEEYGLLFGAIPLILAYMILLFRGILIVRKCETAFPAYLVMGLVVMITVQAM
                     LNMFVAVGLFPVTGQTLPMVSWGRTSVLVVSFSIGAILSVSRVVNARIRQEELPEEEK
                     TDEGDRIYGPAKA"
     gene            1475..2587
                     /gene="murG"
                     /locus_tag="PK288_10935"
     CDS             1475..2587
                     /gene="murG"
                     /locus_tag="PK288_10935"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPM19484.1"
                     /translation="MDPQRHRKIIISGGGTGGHIFPAISIANAVREKDHSAEILFVGA
                     EGKLEMEKVPAAGYRIIGLPVSGIKRKSLSGNFSVFLRLMRSLRKSKKILKEFKPDVV
                     VGVGGYASGPLLRQAGKMKIPMLIQEQNSYAGITNRLLARKASVICVAYDGMEKYFPA
                     GRIVKTGNPVRQDFENLERLKPEALSLFGLSAGLPVVLILGGSLGAGSINSCLLENIG
                     LMKVSECQWLWQTGKLYYEKISESLSGSDTRKISVHGFIDRMDMAFAAADIIVSRAGA
                     GTISELCLVGKPVILIPSPNVAEDHQTKNAMALAGRNAALVIPDKEISELLVPEALKL
                     VADRERRESFARNISAMAERNADRRIAEMVIKLAGK"
     gene            2584..3978
                     /gene="murC"
                     /locus_tag="PK288_10940"
     CDS             2584..3978
                     /gene="murC"
                     /locus_tag="PK288_10940"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPM19485.1"
                     /translation="MIDFRNIEGICFVGIGGIGMSALALYFLRGGYVIEGYDRTETPL
                     TRSLAEQGCNITFRDDPVELSSLFTDPGSAGRVIVVYTPAIPADNRIISFFRDRKYRI
                     YKRSEILGEISEHTDTLAVAGTHGKTTISTMLAHLLKQSAVGCSAFLGGISKNYGTNL
                     LTGESRYTVMEADEFDRSFHRLSPLMAVITSVDADHLDIYGSQVALIEAYNEFAARIR
                     RGGILVLNARIRRSIIVPEGIKCFTYSSSENADYHAMNIRHDSRGYIFDLQVPGGVIR
                     EIVFPFPGVINIENLTAAAAVALNCGVEEYEIRRAALLFQGVRRRFDIRVNMPGLTYV
                     DDYAHHPEEIKAFVTSVREYFRGRKITGIFQPHLFSRTRDHAAGFASTLDELDSVMLL
                     PVYPAREKPIPGVTSELIFNMMKLAEKRLVTMDELPAIIDPGKTDVLLTIGAGDIDRL
                     VLPIEEKLKKAMEK"
     gene            4002..>4653
                     /locus_tag="PK288_10945"
     CDS             4002..>4653
                     /locus_tag="PK288_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19486.1"
                     /translation="MIPTIYLAGIPAYYASTFGRKTCSALEITITNSSDYHFVTQQDI
                     RNTILAWNGNPVGKLSKNIKLADIEETMNTRYRELKTAEAYMSVDGTLHLAADQRTPI
                     LRIMPDEGGDYFLDTEGVVVRRRNLYTPRLHIAGGNINISRAMLNGVSVLDTSIKKTI
                     LRDIYYLIGYINSNSFWSALIDQIYVDSNDEIILVPRGGSHTIHLGTIDNFEEKLRN"
BASE COUNT         1249 a         1026 c         1233 g         1145 t
ORIGIN      
        1 tgattattcg gaacttttcc ctgtcgttaa acagaaggtg aaagccatag tgtgccttgg
       61 cagggacaac aggaaaatta ttgaggcctt cacggacaag gtgccgacga tagtcgaggc
      121 aggttcaatg gaggaagctg tgagatcggc ctattacctg gctaagaagg gacagaccgt
      181 attgctttct cctgcatgtg caagcttcga tctgttcaat aattatgaag acaggggaag
      241 gcaattcaaa acggctgtaa ggaatcttta aatactatgg caatgcaatc cgtcaggcta
      301 aggaacgcct tcaagggcga cagggtaatt tgggccctgg ttgccgtgtt tatgttctat
      361 tcattgctgg cggtttacag ctcatcagcc agtgtggctt acctgaaatt tggcggaaac
      421 aatacctatt tcctcaggag ccagtcgctg atgcttttta tgagtcttat cattatagtc
      481 attgttcatt acctgccata caggatctat tccaggctgt cggggatcat tctcctgggt
      541 tccattgcac tgctggttct caccttcctg ataggagcca gggtaaatga ggcggtcagg
      601 acaattgaaa ttcccggtac cggtttcaga cttcagactt cggatgtggc gaaggttgca
      661 ctggtaattt accttgcacg agccttgtcg cagttcaacg acgaactagg caacttcatg
      721 attgtcacca agtacatgat gcttcccatt gctgtggttt gcggccttat aatgcctgaa
      781 aacctttcca ctgccctgat ggttttttta atatccatga tcatcctgtt tatagggaga
      841 gtaccgctca ggttcctttt ggcctatgca ggcatcgctg tggccggcgt tgttctgttt
      901 gccatggttt taagcatggt gtcggaaaag aacagggtgg aagtatggaa aaccaggatc
      961 gaaagatatt tctcggaaga taccaatgat gaggcagatt atcagtcgaa tcaggccaag
     1021 atagcaattt caaccggagg actgttcgga aaagctcccg gtaagagcac ccagcgcaac
     1081 atccttccgc aatcaaattc cgactttatt tttgcaatta ttatagaaga atatggattg
     1141 ctttttggtg caatcccact gatcctcgca tatatgatcc tccttttcag ggggattttg
     1201 atagtcagga aatgtgagac agcctttccc gcctaccttg tgatgggcct ggtggtgatg
     1261 atcaccgtcc aggcaatgct gaacatgttt gttgctgttg gccttttccc ggtaacgggc
     1321 cagaccttgc ccatggtcag ctggggaaga acatcggtgc tggttgtaag cttctcaatc
     1381 ggggcaatac tcagtgtgag cagggtggtg aatgcaagga taagacagga agaactgccc
     1441 gaagaagaaa aaactgatga aggagacagg atatatggac ccgcaaaggc ataggaaaat
     1501 cataataagc ggcgggggta cgggaggaca tatatttccc gcaatttcca ttgccaatgc
     1561 ggtcagggaa aaggatcact ctgccgagat cctttttgta ggtgcggagg gaaagctgga
     1621 gatggaaaaa gtacccgccg caggctacag gatcatcggc ctgcctgttt ccggaataaa
     1681 aaggaagagc ctgtcaggta atttctctgt attcctcagg cttatgcgca gcctgaggaa
     1741 atcgaagaaa atactgaagg aattcaaacc cgatgttgtg gttggcgttg ggggttatgc
     1801 gagcggtccc cttctccgcc aggcaggtaa aatgaagatc ccgatgctca tccaggaaca
     1861 gaacagttat gcaggtataa caaacaggct gctggcaagg aaagcatcag tgatatgcgt
     1921 cgcatacgac ggtatggaaa aatattttcc tgccggcagg attgtgaaga caggcaatcc
     1981 cgtccgccag gattttgaga atcttgaaag gttgaaacct gaagccctta gcttattcgg
     2041 cctttcagcc ggcctgccag tggtgcttat tcttggaggg tcgcttggcg cgggaagcat
     2101 aaacagctgc cttcttgaaa acatcggatt aatgaaagtt tctgagtgtc agtggctctg
     2161 gcaaacggga aagctctact acgagaaaat atcggagagc ctgtcagggt cggataccag
     2221 gaagatttca gtgcatgggt tcatcgacag gatggatatg gcttttgccg cggcagatat
     2281 aatcgtgtca agggcggggg cgggaacaat ctcagaatta tgtctggtcg ggaaaccggt
     2341 aatactgata ccgtcgccta atgttgcgga agatcaccag acaaaaaacg caatggccct
     2401 tgccgggcgg aatgccgcac tggtgatacc cgataaggaa atcagtgaat tgcttgttcc
     2461 cgaagccctg aagcttgtcg ccgacaggga gcggagggaa agctttgcac ggaatatctc
     2521 agcaatggcc gaaaggaacg ccgataggag gattgctgaa atggtaatta aactagcagg
     2581 gaaatgattg atttcagaaa tattgagggg atctgttttg ttggcatcgg cgggatcgga
     2641 atgagcgccc tggccctcta tttcctcagg ggagggtatg ttattgaggg ttatgacagg
     2701 acggagactc ccctgaccag gtcactggct gaacagggat gtaacataac cttcagggat
     2761 gatccggtgg aattgtcatc tttattcacg gatcccggat cagccggcag ggtgattgtt
     2821 gtttatactc ctgcaattcc tgccgacaac aggatcattt cctttttcag ggacaggaag
     2881 taccggatat acaagcgatc ggagatactt ggagagattt cggagcatac cgataccctg
     2941 gcagttgccg gaacccatgg caaaaccacg atatcaacaa tgttggccca tctgctgaag
     3001 caatccgctg tcggttgctc tgcattcctg ggcggaatat caaaaaacta cggcaccaac
     3061 cttctgacag gcgaaagcag atacaccgta atggaggctg atgagttcga caggtcgttt
     3121 cacaggctta gtccgctcat ggcagtgatc acttcggtcg atgcagacca tcttgatatc
     3181 tatggcagtc aggtggcact tattgaggca tacaatgaat tcgctgcaag gatccgccgc
     3241 ggtggaatcc ttgttctaaa tgcccggatc aggaggagca taattgtgcc ggaagggatt
     3301 aaatgcttta cctacagttc gtctgaaaac gctgactacc atgcgatgaa catcaggcat
     3361 gacagtagag gttatatatt tgatttgcag gtccccggag gggttatcag ggaaatagtc
     3421 tttccgtttc cgggggtcat caatatagag aaccttactg cggctgcagc tgttgcactc
     3481 aactgcggcg tggaggaata cgagattcgt agggctgccc tgctttttca gggagtcaga
     3541 agacggttcg acataagggt caatatgccc gggctgacct acgttgatga ctatgcccac
     3601 cacccggaag agattaaggc atttgtaacc tcggtaaggg aatatttcag gggaagaaaa
     3661 atcacgggta ttttccagcc tcatcttttt tcacgcaccc gcgatcatgc cgcaggcttt
     3721 gcctcaaccc tggatgaact tgactcagta atgctgctac ccgtttatcc ggccagggag
     3781 aaacctattc ccggcgtcac atctgaattg attttcaata tgatgaaact ggcggaaaaa
     3841 aggcttgtca ccatggatga actgccggca ataatcgatc cgggaaaaac agatgtgctg
     3901 ctgacaatcg gggcaggcga catcgacagg cttgtgttac cgattgaaga aaaactcaaa
     3961 aaggcaatgg aaaaatgaaa tggccggtca ggatattgtt aatgatcccg acaatctacc
     4021 ttgccggaat tcctgcctat tatgccagta cgttcggcag gaagacctgc agtgcattgg
     4081 agattaccat taccaattca tccgattatc attttgttac acaacaggat atccggaaca
     4141 ccattcttgc atggaacgga aaccctgtcg gtaaactttc aaaaaacata aagctcgccg
     4201 acatagagga gaccatgaat accagatacc gtgaactgaa aacggctgaa gcctacatgt
     4261 cggttgacgg gacactccat ctggctgctg atcagaggac cccaatactg aggataatgc
     4321 cggatgaagg cggtgactat ttcctcgaca cggagggagt ggtggtaaga cgcaggaatc
     4381 tttatacccc gaggctccat atagcaggcg gcaatataaa tataagccgg gcaatgctca
     4441 acggggtgag cgtactcgac accagtatta aaaaaaccat actcagggac atctattacc
     4501 tcatcggtta tataaacagt aacagttttt ggtcggcact gatcgatcag atctatgtcg
     4561 acagcaatga tgagatcatt cttgttccac gcgggggaag ccatacgata catctcggca
     4621 ccattgacaa ttttgaggaa aagctccgga acc
//
LOCUS       DAOOYG010000174         4632 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_21696_length_4632_cov_5.544243, whole genome shotgun sequence.
ACCESSION   DAOOYG010000174 DAOOYG010000000
VERSION     DAOOYG010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21696_length_4632_cov_5.544243"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PK288_10950"
     CDS             <1..360
                     /locus_tag="PK288_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPM19487.1"
                     /translation="GPKIALSIKSYLNNPENIELINRLRSAGVQLESEAEEAATGNVL
                     RGKNIVISGVFTKHTRDEYKEIIGKYGGRNVSSVSSSTSFILAGENMGPSKRAKAIEL
                     GIEIKNEEEFLKIIGEI"
     gene            407..976
                     /locus_tag="PK288_10955"
     CDS             407..976
                     /locus_tag="PK288_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19488.1"
                     /translation="MELFRNLRIKAGRSILAGKISKVNRRPYYTNFRSVKSMGLVWDA
                     SRPEEFMLLSRFHQKMSEHNIEFRILGLFPGKVLPNQFTAVRYLTCLRKNEIDFFYRP
                     DSKDATVFIEKQFDVLIDINFQKLFPLVYITTLSRARFKVGLADAEPEKSPFDLMLSI
                     NKPVNIENYLEQIILYLDMINSESSKKAV"
     gene            990..1874
                     /gene="dapA"
                     /locus_tag="PK288_10960"
     CDS             990..1874
                     /gene="dapA"
                     /locus_tag="PK288_10960"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPM19489.1"
                     /translation="MKKIKGTGVAIVTPFKNDTSIDFSALKKITEHVIGGGVNYIVVM
                     GTTGESVTLTKDEKKAVLSYVLEVTDKRVPVIAGIGGNNTQEVINCLRHSNLSDVDAI
                     LSVVPYYNKPGQKGIFQHFKTIAACSPVPVILYNVPGRTSCSMSAETCLELAHECENI
                     AGIKESSGDMSLVTKIIKGKPENFVLLSGDDMLSIPIISLGGSGVISVLANAFPSQCS
                     EMIGHALKSNFRTAREHHFRLVELIDLCFAEGNPSGIKAMLSTLNLSHNVLRLPLVPV
                     SRPLMSRIQKAVEDFNKQ"
     gene            complement(1881..>4632)
                     /gene="secA"
                     /locus_tag="PK288_10965"
     CDS             complement(1881..>4632)
                     /gene="secA"
                     /locus_tag="PK288_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPM19490.1"
                     /translation="EMATGEGKTVVATLPVFLNALAGRGVHIVTVNDYLSKRDSEWMG
                     PIYEFHGLTVDCIDKHLPNSAQRRNAYHADITFGTNNEFGFDYLRDNMAINPEDLVQR
                     RHHYAIVDEVDSVLIDDARTPLIISGPTAKSDTKQFDELKPKVDKLVSAQKKLVTQIL
                     ADSKRLIADNNTEEGGKLLLRAYKGLPKNNALIKFLSEEGNKTLLQKTENFYMQNNSK
                     EMPKITEELYFVIDEKANSIELTEKGLDLISTSVEDASFFVLPDVGSKIADLEKSGLS
                     DREKFRKKDELLQDYAVKSERVHTMSQLLKAWTLFDRDVEYVVIDNKVKIVDEQTGRI
                     LEGRRYSDGLHQAIEAKENVKVEEATQTYATITLQNYFRMYHKLAGMTGTAETEAGEF
                     WNIYKLDVVVIPTNRPVIRNDREDLVYKTKREKYNAVIDEIVSLNKQGRPVLVGTTSV
                     EISELLSRMLKMKGLKHNVLNAKLHQREAEIVAEAGKSGTITIATNMAGRGTDIKLTP
                     EVRAAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGSSQFFVSLEDELMRLFGSERIA
                     GIMDKLGLKDGEMIQHPMITRSIERAQKKVEENNFGIRKRLLEYDDVMNSQREVIYKK
                     RRHALLGERLSVDVANMMYDVTENLVDTYHREGDFENFDFDLIRSFSMDSPVPQQDYL
                     KSSSEQVTEKVYEKVLDTYKRKVEMISQQAYPVIRDVYERMSHAYENVVVPISDGYRV
                     FQVVTHLKTTAETEGRELARAYEKTVVLATIDDAWKEHLREMDELKQSVQNATYEQKD
                     PLLIYKFESFELFKTMIAKVNKDVVSTLMKGHIPTQDPEQVKEAQRRRQVDMSKLRTS
                     KSDFSQYSSTGGSGQDKNQKVEPVRVEKKVGRNDPCPCGSGKKYKQCHGSQQPVSQGL
                     RV"
BASE COUNT         1232 a         1075 c          999 g         1326 t
ORIGIN      
        1 ggtcccaaga ttgcattaag tataaaatca tatttaaata atccggagaa tattgaactg
       61 ataaacaggc tccggtccgc cggagttcag cttgaaagcg aagctgaaga ggccgccacg
      121 ggaaatgtgc tgaggggtaa aaacattgta atttcaggtg ttttcacgaa gcatacgagg
      181 gatgaataca aggaaataat cgggaaatac gggggaagga atgtatcgtc ggtcagttcc
      241 tccacctcct ttatccttgc cggagagaat atgggaccat caaaaagggc aaaggcaatt
      301 gaactcggaa ttgaaataaa aaatgaggaa gaatttctga aaatcatcgg tgaaatataa
      361 gcaatcctgc ttgtcgattt tgacaaaaaa ctgttaggtt tgcaccatgg aattattcag
      421 gaatttaagg ataaaggcgg ggagatcgat actggccggg aagatatcaa aggtcaacag
      481 gagaccatac tatactaatt tcagaagcgt aaaaagcatg ggattggtat gggatgcatc
      541 acgacctgag gaattcatgt tgttgtcgag gtttcatcag aaaatgtcgg agcataacat
      601 cgaattcagg attctgggtt tattccccgg gaaggtatta ccaaatcagt tcactgccgt
      661 cagatatctg acctgtctca ggaaaaacga aattgatttt ttctaccgcc ctgattccaa
      721 ggatgccacg gtgtttattg agaagcaatt tgacgtactt atagacatta atttccagaa
      781 actatttcct cttgtttaca taacaacatt atcccgggcg cgatttaagg ttgggttagc
      841 tgatgctgag cctgaaaaat caccgttcga ccttatgctg tcaataaata aacctgttaa
      901 tatcgaaaat tacctggaac agatcatctt atatcttgac atgataaatt ctgaatcatc
      961 caaaaaagct gtttaatacg gggctaaaaa tgaagaaaat caaaggtaca ggtgtggcaa
     1021 tagtaacgcc attcaaaaat gacacgagta ttgatttctc ggcattgaaa aagatcaccg
     1081 agcatgtgat cggcggaggt gtgaattaca ttgttgtgat gggcacaact ggtgagtctg
     1141 tcactctcac taaagatgaa aagaaggccg ttctgtcata tgtacttgaa gtaacagaca
     1201 aaagggttcc ggtaattgcc gggatcggcg ggaataatac ccaggaagtc ataaactgcc
     1261 tcaggcattc caatctcagt gatgttgacg ccatcctgtc agttgttccg tattacaata
     1321 aaccgggtca gaaggggata tttcagcact ttaaaaccat tgccgcatgc agccctgttc
     1381 cggttatact ttataatgtc cccggcagga ccagttgcag catgtcagct gaaacatgtc
     1441 tcgagctggc tcacgaatgc gaaaacatag cagggataaa ggagtcgtca ggcgatatgt
     1501 cgctggtaac taaaataatc aaggggaaac cggagaattt tgtcctgctt tcgggcgatg
     1561 atatgctttc aattcccatt atttcactgg gaggatcggg tgtaatatcg gttcttgcca
     1621 atgctttccc gtcgcaatgc agtgaaatga tcggtcacgc cctgaaaagt aatttcagaa
     1681 ctgcaaggga acatcatttc cgtttggttg aactgatcga tctttgcttt gccgaaggaa
     1741 atccatcggg gataaaggca atgctgagta cccttaacct ttcgcacaac gtcctgaggc
     1801 ttccgctggt tcctgtcagc aggccgctga tgagcaggat ccagaaggca gttgaggact
     1861 ttaacaaaca gtgattgatc ttataccctt aaaccctggg atacaggctg ctggcttccg
     1921 tgacattgtt tgtacttttt tccgcttccg caaggacatg gatcattgcg gcctactttt
     1981 ttctcaacac gaacaggttc aaccttctga tttttatcct ggccgcttcc tcccgtgctg
     2041 ctgtattgcg agaagtcgct ttttgacgtc ctcagcttgc tcatgtccac ctgccgcctg
     2101 cgctgggctt cctttacctg ctcaggatcc tgggtgggaa tatgaccttt catgagagta
     2161 ctcacaacat ccttatttac cttggcaatc attgtcttaa aaagctcaaa tgattcgaac
     2221 ttgtaaataa gcaggggatc cttctgttca taggtggcat tctgcaccga ctgcttaagt
     2281 tcatccattt ctctcaggtg ttctttccag gcatcgtcaa tagttgccag tacaacggtt
     2341 ttttcatatg cccgggccag ttcccgccct tccgtttcag cagttgtttt gagatgggtc
     2401 accacctgga acaccctgta tccgtcagaa ataggaacaa caacattttc atatgcatgc
     2461 gacatccttt cataaacatc cctgattacc ggataggctt gctgtgaaat catttcaacc
     2521 ttccttttgt aagtatcaag gactttttca tagactttct cagtgacctg ttctgaactg
     2581 ctttttaaat aatcctgctg gggaactggc gaatccatcg agaatgaacg tatgagatca
     2641 aaatcgaagt tttcaaagtc accttctcta tggtatgtat ccacaaggtt ttcagtaaca
     2701 tcatacatca tgttcgcaac atcaaccgaa agcctttctc cgagaagtgc atgcctcctt
     2761 ttcttataga tcacctctct ctgtgagttc ataacatcat cgtactcaag aagccgcttt
     2821 ctgattccga agttattctc ttccactttc ttctgagccc gttctatcga cctggttatc
     2881 attggatgct ggatcatttc cccatccttt aaaccaagct tatccataat tccggcaata
     2941 cgttccgagc cgaacaacct cataagctca tcctcgaggg aaacaaagaa ctgggatgat
     3001 ccgggatcac cctgtcttcc tgaccgaccc ctgagctgac ggtcaacacg tcttgattca
     3061 tgacgctctg ttccgataat tgcaagcccg ccggcggcac gaacttccgg agtaagttta
     3121 atgtcagttc cacgtcctgc catattggtg gcaatcgtta tcgttcccga ttttccggct
     3181 tccgcaacaa tctcggcttc ccgctgatga agcttggcat taaggacgtt atgcttcagc
     3241 cccttcatct tcagcatcct gctgagcaat tccgaaattt caacggaggt agttccaacc
     3301 agaaccggac gaccctgttt attcaggctt acaatctcgt caattactgc attgtacttc
     3361 tccctttttg tcttgtaaac cagatcttca cggtcgtttc ttattaccgg acggttggta
     3421 gggattacca caacatcaag tttataaata ttccagaact caccggcttc agtttctgca
     3481 gtaccagtca tgcctgcaag cttgtgatac atccggaaat agttctgcag ggtaattgtt
     3541 gcatatgtct gggtggcttc ctccacttta acattttcct ttgcctctat tgcctggtga
     3601 aggccgtcgg aatagcgccg gccctcaaga atccttcccg tctgttcatc gacaattttt
     3661 accttattgt cgataacaac atactcaaca tcccggtcaa acagggtcca ggctttaaga
     3721 agctgagaca ttgtatgcac cctttcagat ttaacggcat aatcctgaag cagttcgtcc
     3781 tttttcctga acttttcccg gtcgctgagc ccggatttct ccagatcagc aatttttgac
     3841 ccaacatccg gaagcacaaa aaatgaagca tcctcaacag aagtggatat cagatcgagg
     3901 cccttttcag tcagctcaat ggaattggct ttttcatcaa tcacaaaata aagttcttcc
     3961 gtgattttag gcatttcctt gctgttattc tgcatataga agttctcagt cttctgcaga
     4021 agggtcttat tgccctcttc actcaggaat ttgataagag cattgttctt tggcaaaccc
     4081 ttatatgccc tcagaagcaa tttgccaccc tcttcggtgt tattatctgc aataagcctc
     4141 ttggaatcgg caagtatctg tgttaccagt tttttctgcg cactgacgag tttgtcaacc
     4201 tttggtttaa gctcgtcaaa ctgcttggtg tcgcttttgg ctgtcggacc tgagataata
     4261 agaggtgtac gtgcgtcatc gataagaacc gagtcaactt cgtcaacaat ggcataatgg
     4321 tgacggcgct gcacgagatc ttcggggtta attgccatat tgtccctcag gtaatcaaat
     4381 ccgaattcat tgttggtacc aaatgtaatg tcggcatgat aggcattcct cctttgggcc
     4441 gaatttggca aatgtttgtc aatacaatca accgtcagac cgtgaaactc atatatcggg
     4501 cccatccatt cggagtcgcg ctttgaaaga tagtcgttta cggtcacgat atgcacgcct
     4561 ctgccggcaa gggcattaag aaaaacgggc agagtggcaa caaccgtctt accctctccg
     4621 gtggccattt ca
//
LOCUS       DAOOYG010000175         4630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_21709_length_4630_cov_6.953224, whole genome shotgun sequence.
ACCESSION   DAOOYG010000175 DAOOYG010000000
VERSION     DAOOYG010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21709_length_4630_cov_6.953224"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK288_10970"
     CDS             complement(<1..423)
                     /locus_tag="PK288_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HPM19491.1"
                     /translation="MQYRYKIAGGDFSNAGAVASSVKKTLRQLNIDHRLSKRIVIALY
                     EGEVNVVAHAYSGIIDIDIDHERIKIRITDKGPGIVDIAKAMEEGYSTASTQVREMGF
                     GAGMGLSNMKKNSDSMQITSEPGIGTVVELTTYLNGNEG"
     gene            complement(426..770)
                     /locus_tag="PK288_10975"
     CDS             complement(426..770)
                     /locus_tag="PK288_10975"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPM19492.1"
                     /translation="MLITEVASKIEGRVVCCENKAAGEVEYAFSSDLMSDVLKVEREN
                     VLLITGLANLQVIRTSEMSDINCIVIARNKKLNSEIIKLATENGIVLIESPYSVFRIS
                     GELYKAGIKPLF"
     gene            complement(1121..3646)
                     /locus_tag="PK288_10980"
     CDS             complement(1121..3646)
                     /locus_tag="PK288_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPM19493.1"
                     /translation="MREFVFIFLISTLISVNIFCQPVKSPDEFLGYELGTQFTFHHRA
                     VDYFRYIAGQSECAEIIEYGTTYEGRPLCVCVVSEKANLAILDQYRNNNLIKAGLVKG
                     DIAGSQLPIIWLAYNIHGNEAAGMETAMKTLYTLVSGSFPGSENWLRSCIIIIDPCQN
                     PDGHELYVNRYRSSQNLHPNADGNSWEHNQGWPGARTNHYFFDLNRDWAWQTQVETVE
                     RLTLYRKFMPHVYADFHEMGSGSTFFFAPGADPWHEAITPWQRKFHEIMGKGNAALFD
                     EKSKLYFTKEHYDLFFPAYSDTWPLFNGAAGFTYEQGGSGYAGLALRQESEDTLTLKK
                     RIDGHFTASMATLKVSYENREKLIAEFNTFFRENAENPAFEFKSIIIKGSNDKTNLND
                     LLLLLDRNQIRYSPAGNTGKKYRGFDYLANVQGETTIEAGDILVSAFQPQARLVKVLF
                     EPDSRITDSLSYDLTAWALPYTFNVKAFAIREKIVPGPKISEKNSPGNMPPQNPDPYA
                     YLVNFSGFSEMKLVSALQEKNIKVRHCFKPFSVNGTSFERGSYIITKADNRIAGENLD
                     RIVTGLAGRLGVVLKPVSTGLVDEGKDLGSDYSPLLKKKKIALLCGDGTSSGAVGEIW
                     YFFEREVDYPVTVINTSNAERTDLTSYDVLILAGGSYSKLNDIIIDFARKGGRVIALE
                     NSISVFASEKSTSLYKDNEARKTELKASADREKNDASLLLRKFEDERRYFLSERSAGS
                     IYRVRIDSSHPYGFGLGNEWFVIKGSSSYPLLSKGSNVGYIPENDPVAGFAGYKFRKQ
                     VKNTLVIGTEKIGEGEVVYIADNPYFRAYWKSGRILLWNVIFR"
     gene            3810..>4630
                     /locus_tag="PK288_10985"
     CDS             3810..>4630
                     /locus_tag="PK288_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPM19494.1"
                     /translation="MKANISSGFLIVFISAILSSMAFAQTGMQPRGANQVRVTGWKDD
                     NHYLIQAYDEQRNLVLKSVDIRTGTAVEVQPERTAREVLSGLLPEGTMLGYGDPVSSD
                     GRSAIITKDNDLFYFNIDDRKLRRLTNDSRQEVNPRFSDDGKRIAYTRNRDLYVYDLE
                     NGIETRLTHDASERIYNGYASWVYMEEILGRQSNYAAFWFSPDGRKIAYLRTDETDVP
                     VFTLNRLDEPDGIHGVLEQVPYPKAGDPNPRVKMGIADISTGKTTWVKTDETIDQ"
BASE COUNT         1182 a         1072 c         1009 g         1367 t
ORIGIN      
        1 cccttcattg ccgttaaggt atgtcgtaag ctcaactacc gtaccgatgc ctggttcact
       61 tgttatctgc attgagtcgc tgttcttctt catgtttgaa aggcccattc ctgctccgaa
      121 tcccatttcc ctgacctgcg tggaagcggt tgaataacct tcctccattg cctttgctat
      181 atcaactatt ccaggtccct tatcggttat ccttattttt atcctttcat ggtctatgtc
      241 aatgtcaatt atacccgaat aagcatgtgc caccacattc acttcacctt catacagggc
      301 tatcactatt cttttggaaa gccgatggtc aatattgagc tgacgcaggg tctttttgac
      361 cgagcttgcc accgcccctg catttgagaa atcaccacct gctattttat acctgtattg
      421 cattttcaga atagcggctt aatgcctgcc ttgtataatt ctcccgaaat acggaacacc
      481 gaataagggc tttcaatcag aactatccca ttctcggttg caagctttat tatctcactg
      541 tttaactttt tgtttcttgc gattacaatg caattgatat ccgacatttc cgatgtcctt
      601 attacctgaa gattggccag accggtgatc agaagtacat tttccctttc cactttcaat
      661 acatcgctca ttagatctga ggaaaaagca tattccactt ctcctgcagc cttgttttca
      721 cagcagacta cccgtccttc tatcttgctt gctacttcag tgattaacat tgtattatga
      781 taaatcttgc aaaaatattc agttaacaga ccgttgtttt gaccatgcaa agatagtaat
      841 ttattgtgaa tttaataaca gtgttaataa aataacaata aatattattg attcctgccg
      901 gcagcctggc aggaaaccag gaaataatga cgctgtagtt attttttaga aaacaatgcc
      961 tgtttgagaa agaaattcct gtgcggtaaa gtccggcatt cagcaaagaa ccatttcaag
     1021 gggggaaggg cgggagataa atgttttttc ccgcttgctt ttactgtatt ttttgctttt
     1081 gcctttccga ttgcatttca gtaaacaggc tcaggtgaat ttacctgaat ataacattcc
     1141 acaggagtat ccttccgctt ttccagtatg ccctgaagta aggattatcg gcaatataca
     1201 ccacttctcc ctcaccgatt ttctcggttc cgataaccag tgtgttcttt acctgtttcc
     1261 tgaatttata gcctgcaaac cctgccacgg gatcgttttc agggatgtat ccgacattac
     1321 tgcccttgct cagcagtgga taagatgaac ttccctttat gacgaaccac tcatttccca
     1381 ggccgaaccc gtagggatgg ctgctgtcga ttctgaccct gtaaatgctt cctgccgacc
     1441 tttccgacag aaaatatctc ctttcatcct caaatttcct cagaagcaaa ctggcatcgt
     1501 ttttctccct gtctgctgat gccttcagct ccgttttgcg ggcttcatta tccttataca
     1561 gtgaggtaga tttttcggat gcaaaaacgg aaatggaatt ttcaagggca attaccctgc
     1621 ctcctttcct tgcgaaatca ataatgatgt cattcagttt tgaataactg ccgccggcaa
     1681 gaatgaggac atcatagctg gtaaggtccg tacgttcagc atttgatgtg ttgatcacgg
     1741 taacgggata atccacttcg cgctcaaaaa aataccatat ttcgcctacg gctcctgaag
     1801 atgtaccatc accgcacagg agcgcgattt ttttcttttt cagaagtggt gaatagtctg
     1861 acccgagatc ctttccctca tcaacaagac cggttgagac cggtttcagg actaccccga
     1921 gtcggccggc cagtccggta acaatgcggt caagattttc gccggcaatc ctgttatcag
     1981 cctttgtaat tatataacta cctctttcaa agctagttcc gttgacagaa aatggtttga
     2041 agcaatgcct gaccttaata ttcttttcct ggagtgccga tactaacttc atttcactga
     2101 aaccgctgaa attcaccagg taggcgtagg gatccggatt ttggggcggc atgtttcccg
     2161 gtgagttttt ctcagatatt ttcggtccgg gaactatctt ttcccggatg gcaaatgcct
     2221 tgacgttaaa cgtatatggc aatgcccatg cagtgaggtc gtagctcagg gagtcggtga
     2281 ttctgctgtc gggctcgaac agtactttca ccagtcttgc ctggggctgg aaagcactta
     2341 caagaatatc gccggcttct attgttgttt caccctgcac atttgccagg tagtcaaatc
     2401 cacggtattt tttaccggta ttccctgccg gcgagtacct gatctgattt ctgtcgagga
     2461 gcaggagcag atcgttaagg ttcgttttat catttgaccc cttgattatt atggatttaa
     2521 actcaaatgc agggttttct gcattctctc tgaagaatgt gttaaactcc gcaatcagct
     2581 tttccctgtt ttcgtacgat actttcaatg ttgccattga agcggtaaag tggccatcga
     2641 tccttttttt cagggtcagt gtatcttccg attcctgcct gagggccagc cccgcgtatc
     2701 cgcttcctcc ctgttcatag gtaaatcctg cagcgccgtt gaacaggggc caggtatcgc
     2761 tgtaggccgg aaaaaaaaga tcatagtgct cttttgtgaa ataaagcttt gacttttcat
     2821 caaacagtgc tgcattccct ttacccatga tttcgtgaaa cttacgctgc cagggggtta
     2881 ttgcctcgtg ccatggatcc gctcccgggg caaaaaagaa tgtggagccg gagcccattt
     2941 catgaaaatc ggcgtacaca tgaggcatga atttcctgta gagggtgagc ctttcaactg
     3001 tttcaacctg cgtctgccag gcccagtcgc ggttcaggtc aaagaaataa tgattggttc
     3061 tggctccggg ccagccctgg ttatgttccc atgaattgcc atcagcgttg ggatgaagat
     3121 tttgggaact cctgtatcgg ttgacgtaaa gctcatgacc gtcaggattc tggcagggat
     3181 cgatgattat tatgcacgat ctcagccagt tttccgatcc cggaaatgat cctgaaacca
     3241 gggtgtaaag tgttttcatc gctgtttcca ttcctgccgc ttcgttgccg tgaatattat
     3301 aggccagcca gatgattggc agctgactgc ctgcgatatc gcctttgacc aggccggcct
     3361 tgataagatt attgttcctg tattgatcga gtatggcaag attagccttt tccgaaacga
     3421 cgcaaacaca taggggcctt ccttcgtagg tggttccgta ttcaatgatc tcggcacatt
     3481 ctgactgccc ggcaatatat ctgaaataat caactgccct gtgatggaac gtaaactgag
     3541 ttccaagttc ataaccgaga aattcgtcgg gtgatttaac aggctgacaa aaaatattaa
     3601 ccgaaatcag cgtactgatt aaaaatatga aaacaaactc cctcatcagt ttaaaaatta
     3661 taataacgaa cgtcaaatat agtgtttctc agaaacacga catggaaatt ttctgtctga
     3721 ttttttatta accttcaggt tcagcagata ggtaattcaa aatacctatt tttacaattt
     3781 tgtctaatcc ggattaatac gattcagcta tgaaagcaaa tatcagttca gggtttttaa
     3841 tagtattcat atccgcaatt ttgtcgtcga tggcgtttgc acagaccgga atgcaaccgc
     3901 gcggtgcaaa tcaggtcagg gtcactggct ggaaagatga caatcattac ctcattcagg
     3961 cttatgatga acagcggaat ctggtgttga agagtgtgga tatcagaaca ggcacagcgg
     4021 ttgaggtgca gcctgagaga acagccaggg aagtcctttc gggcctgctg cctgaaggaa
     4081 caatgcttgg ctacggcgat ccggtcagtt cagacggcag gagtgcaata attacaaaag
     4141 acaatgatct gttctatttc aatattgatg accggaaact ccggaggctt acaaatgaca
     4201 gccggcagga agttaatccg cgtttcagtg atgacgggaa aagaatcgcc tatacaagga
     4261 acagggatct ttatgtttat gaccttgaga acggcataga aacaaggctt acccatgatg
     4321 cttcggagcg tatatacaac ggttatgcct cgtgggtcta tatggaggag atccttggaa
     4381 ggcagagtaa ttatgcagct ttctggtttt ctcccgatgg caggaagatt gcttatctga
     4441 gaacggatga aacggatgtt ccggtgttca ccctgaatcg tcttgatgaa ccagacggca
     4501 tccacggtgt cctggaacag gtgccgtacc cgaaggccgg tgatcccaat ccacgcgtaa
     4561 aaatgggcat tgcagacatt tccaccggga aaaccacatg ggttaagacc gatgagacca
     4621 ttgatcaata
//
LOCUS       DAOOYG010000176         4593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_21970_length_4593_cov_5.030190, whole genome shotgun sequence.
ACCESSION   DAOOYG010000176 DAOOYG010000000
VERSION     DAOOYG010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21970_length_4593_cov_5.030190"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PK288_10990"
     CDS             complement(<1..861)
                     /locus_tag="PK288_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HPM19495.1"
                     /translation="MGKKYTRLLILIVSCYSILSCNSPPATERNDSVAVRDMLGREVY
                     VPSKINRIVGLRAGALRLLVYMDVIGKVAGVEEFERKGTTPYAMAHPELTLLPSVGPS
                     MGGDAELIVKVQPDVIFITYTTTGDADALQQKTGIPVIALECPELGTARDTLFASLKL
                     IGKILNRKGRADSLIAWINNSIADLNERTAGIPDSERPTVYIGGVPYSGMRGINSTQP
                     FYPPFIFTNAKNVASSIDKRLISHVKGTYIDIEQLMLWNPDMLFIDVSGLSLVRKDLE
                     KGSALSDQLGA"
     gene            complement(863..2920)
                     /locus_tag="PK288_10995"
     CDS             complement(863..2920)
                     /locus_tag="PK288_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM19496.1"
                     /translation="MKKLNRIKSLWIIPLLLASSLNLHSQVYTMDTLLLREVVVTATK
                     TMRNLTEVPARISVVKSGLIESTPLLQVDDILRYTPGINVNRSSGIYSQRPMVTLRGL
                     SGDEQSRTLVLVNGVPVNTSDEGGVNWNRINQFDIERIEVFKGPGSSLYGNNAMGGVI
                     NLITRKPSRPQEILGAVSYGTFNTLRQDLNISVRSDKGYYGTVSQYFLKSDGYNNIVK
                     EKRTPYDIARTLEEIGVSARAGFDKSTWFNWELQYDIFRDKRGEGYQIYTPEGCYRNF
                     NTNLFRGNLKGNDGTTRYDLSLYYQLEHYYDINERLRGPNYSRYDVNSFRKDMGTLFN
                     ISRDLSSKNTLTAGFELKQGSIEGGDYYQTPRQTGPSTQVYDTIFNAGKIKTVAAYAQ
                     DEHSFLDGKIRFIAGLRYDRVSFSDGEFKSTDPWNVTPELKDHTWSELSPRLGLRLNF
                     IEEVSAYLSYSHGFRASILDDLTRTGYMWVGPKYANPELGPESLDNYEIGLDMVPAKK
                     LKFSASVYYARGKDFLYYVSTGDSLFGRPIYIRKNVTNVYIKGAEAEIMYEIANGFDL
                     MANYSYTDARIDKFAERPDLVNKYLKYSPKHIASASLFWKNRIVNASIRGLYKGEQFS
                     DDANAQKLDGYFTMDLMLSRDLFKNFNVSLDIQDLFNNRRMETGEYISPGRLITGRVS
                     FKF"
     gene            complement(2987..3388)
                     /gene="nikR"
                     /locus_tag="PK288_11000"
     CDS             complement(2987..3388)
                     /gene="nikR"
                     /locus_tag="PK288_11000"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPM19497.1"
                     /translation="MSVIRFGVSLEREVLAALDNYTKDNRIKNRSQAIKHLINNNLVE
                     KKWHCNNVVAGSITLVYNFHKHDLLANLTAIQHDYHTTILSSQHFHLSHKSCMEIIAV
                     KGKAKILTELAEKLIAVKGIQHGKLTMSRAD"
     gene            3630..3977
                     /locus_tag="PK288_11005"
     CDS             3630..3977
                     /locus_tag="PK288_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018910648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HPM19498.1"
                     /translation="MITLRRVYDPEKPGEHYRVLVDRLWPRGISKDNVTWDEWMKEVG
                     PSNQLRKWFGHDPSRWEKFKSMYREELGSRQDLLKKLRQLEEKHGTLTLLYSSREHEY
                     NNAVALRELLMQT"
     gene            complement(4083..>4593)
                     /locus_tag="PK288_11010"
     CDS             complement(4083..>4593)
                     /locus_tag="PK288_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPM19499.1"
                     /translation="PRTGIFEINLPPMQPGSSIMPGKVNPVIPEVVNQIAFKVIGNDL
                     TVTLASEAGQLELNVFEPVIIQSIFESVEMLMNGMNTLKYRCIDGITANEERCRQMVM
                     NSIGLITALVPYIGYDISGKLAKEAIEKNVSLYSLILDKGILSEEKLNQVLMPENMIK
                     PVKTNGLIR"
BASE COUNT         1146 a         1028 c         1093 g         1326 t
ORIGIN      
        1 tgcgccgagc tgatctgaca atgcgctgcc tttttccagg tctttcctta ccaggctgag
       61 ccccgacaca tcaatgaaaa gcatatcggg gttccagagc atcagctgtt ctatatctat
      121 ataggtaccc ttcacatgcg atataagcct tttatcgatg gatgaggcaa cattttttgc
      181 gttcgtgaag ataaagggag gatagaaggg ctgggttgaa ttaataccac gcatccctga
      241 atatggcaca ccgcctatat aaaccgtggg cctttctgaa tcgggaattc ctgccgtgcg
      301 ttcattcaga tctgcaattg aattatttat ccaggcaatg agagaatcgg ctcgtccttt
      361 tctgttcagt atttttccga tcagtttgag ggaagcaaac agtgtatctc tcgcagtccc
      421 taactcgggg cattcaaggg caattaccgg tatgccggtt ttttgctgca gtgcatcagc
      481 atcgccggtt gttgtatagg taatgaatat gacatccggc tgtaccttta ctatcagttc
      541 ggcgtcacct cccattgacg ggccgaccga gggtaacagt gtcagttccg gatgtgccat
      601 cgcgtagggc gttgtaccct ttcgttcaaa ttcttcaact ccggcaacct tgccgataac
      661 atccatataa accagaaggc gcagggcgcc ggcccttaac ccgacaattc tgttaatctt
      721 tgacggaaca taaacttccc ttccgagcat gtcccttacc gcaaccgagt cattcctttc
      781 agtcgcgggc ggactattgc aacttaatat tgagtagcat gatacaatca gtatcagaag
      841 ccttgtatat tttttcccca tttcagaatt taaatgaaac acgaccggtt attaaccttc
      901 cgggcgagat atattcacct gtttccatac gccggttatt aaacaggtcc tgaatatcca
      961 atgatacgtt aaaattttta aaaagatccc tcgaaagcat cagatccatg gtaaaatatc
     1021 catcaagttt ctgtgcattg gcgtcatcgc tgaactgttc tcccttgtaa agacccctga
     1081 tgcttgcatt aacgattctg tttttccaga agagggaagc agaggctata tgtttggggg
     1141 agtacttcag atacttattt acaaggtcgg gtctttcggc aaacttatcg attcttgcat
     1201 cggtgtatga gtagttagcc ataagatcga agccgtttgc aatttcatac atgatctccg
     1261 cttccgcgcc tttaatatat acattggtaa cgttcttccg gatatatatc gggcggccga
     1321 acaggctgtc gcctgttgac acataataaa gaaaatcttt gccgcgtgca taataaactg
     1381 atgcagaaaa cttcagtttt tttgccggta ccatatcgag gcctatttca taattgtcga
     1441 gtgattcggg acccagctcg ggattggcat acttcgggcc cacccacata tagccggtac
     1501 gtgtgaggtc gtcgagtatc gaggccctga aaccgtgtga atatgaaaga taggcactaa
     1561 cttcctcgat gaagttcagc cgcaggccca accgtggaga aagctctgac caggtatggt
     1621 cttttagttc aggtgtaacg ttccatggat cagtcgattt gaattcaccg tcggagaagc
     1681 ttacccggtc gtagcgtaaa ccggctatga atcgtatttt accatccaga aaggaatgct
     1741 cgtcctgggc atacgctgca acagttttta ttttgcctgc attgaaaata gtatcgtaaa
     1801 cctgggtgga gggacctgtc tggcggggag tctggtaata gtcaccgcct tcaatgctgc
     1861 cctgctttaa ttcaaaacca gccgttaagg tattttttga cgagaggtcc ctgcttatgt
     1921 tgaacaatgt tcccatatcc tttctgaatg aattcacatc gtaacgtgaa tagttcgggc
     1981 cccgtaatct ctcattgata tcataataat gctcaagctg gtaatatagg ctcagatcat
     2041 aacgagtcgt gccgtcattc cccttgaggt ttcccctgaa cagattcgtg ttgaaattgc
     2101 ggtagcatcc ttcaggggtg tagatttgat aaccttcacc tcttttatcc ctgaagatat
     2161 catactgcag ttcccagttg aaccaggtgc ttttgtcgaa accggccctg gccgatacgc
     2221 caatctcttc cagtgtcctg gcaatatcat aaggggtccg tttctctttg acgatattgt
     2281 tgtatccgtc gcttttcagg aaatactgcg atacggttcc atagtatccc ttgtcggacc
     2341 tgacgcttat gttcaggtcc tgcctcaggg tgttgaatgt accgtagctt actgcgccga
     2401 gtatttcctg gggcctggaa ggctttctgg taatcaggtt gatcacgccg cccattgcgt
     2461 tattgccata gagtgaagag cccggtcctt tgaagacttc aattctttca atatcaaact
     2521 ggttgatcct gttccagtta acaccaccct catcggatgt gttgacagga accccgttaa
     2581 caagcacaag tgtccttgac tgttcatcac ccgacaatcc gcgcagggta accatggggc
     2641 gctggctgta gattccggaa gacctgttca cattaatgcc gggtgtgtag cgcaggatat
     2701 cgtcgacctg tagtaaggga gtggattcaa tcaggccgct tttaacaacc gagattctgg
     2761 caggcacctc ggtgaggttg cgcatcgttt tggtagcggt cacaacaacc tcgcgaagca
     2821 gaagagtgtc catcgtgtaa acctgagaat gcagattcag ggatgaagcc agtaaaagcg
     2881 gtataatcca cagggatttg attctgttaa gttttttcat ggaaaaagat ttaattacaa
     2941 aggtgttaca attaatatat tcgtgttact atctaatcaa aaaaaatcag tccgccctgc
     3001 tcattgtcag ttttccatgc tggattccct tgactgcaat gagtttctca gccagttcag
     3061 tcaggatttt tgcttttccc ttcacggcaa ttatttccat gcagctttta tggctgagat
     3121 gaaaatgctg tgaggaaaga atggtcgtat ggtaatcatg ttgtatggcg gtaaggttgg
     3181 ccagcagatc atgcttatgg aaattgtaaa ccagggtgat tgatccggca acgacattgt
     3241 tgcaatgcca tttcttttca accaggttat tgtttatcag gtgtttgatt gcctgggacc
     3301 tgttcttaat acggttatct ttggtgtaat tatccagggc cgcaagaact tcccgttcaa
     3361 gagaaacgcc gaatcggatc acggacatgg tgttactttt tattttttcg tgatacaaca
     3421 ggcaaatata gtttattttt aatacgtgcg gcttccggtg catggcagga tcgttttttt
     3481 aatgattttt aaatattgca gaggcgggta tctggactgt tggatcagtt tatccggatt
     3541 cccgcgggag gcgcagtcga aattaccgct gatattgaac aaattgccgc atttgttgtt
     3601 tgattcatga ttcttaaaca accagtcaga tgattacgct cagaagagta tatgatccgg
     3661 aaaagcccgg ggaacactac agggtcctgg ttgacaggct ctggcccagg gggatctcaa
     3721 aggacaatgt cacctgggat gaatggatga aggaggttgg accaagcaat cagctgcgca
     3781 agtggttcgg tcatgatcct tcccgatggg aaaaatttaa atcaatgtac agggaagaat
     3841 tgggcagcag gcaggattta ctgaaaaagc tcaggcagct tgaagaaaag catggaaccc
     3901 tgacgctgct gtattcatcg agggagcatg aatacaacaa tgccgttgcg ttgcgggaac
     3961 tgcttatgca gacttgagtt caaccggaaa aaatccggtg aatcattcat atcaggaaaa
     4021 gcggaatcct gccgggatgc atttctgtaa atgtatacag agcttctgca gtctttcttt
     4081 aattaacgga tcaatccatt ggttttgacg ggcttgatca tattttcagg cataagtacc
     4141 tggttgagtt tttcttcgct gaggataccc ttgtcgagga taagggaata gaggcttaca
     4201 ttcttttcaa ttgcctcttt tgcaagtttt ccggagatat cgtagccgat gtagggcacc
     4261 agtgccgtga tcaggccgat actgttcatg accatctgcc tgcacctttc ctcatttgcc
     4321 gtgattccgt cgatacagcg gtatttaagg gtgttcatgc cattcatcag catctcgacg
     4381 gattcgaaaa tgctctggat aattaccggt tcaaagacat tcagttcaag ctgtccggct
     4441 tccgaagcca gggtaacggt aaggtcattt ccgatcacct tgaatgcgat ctggttcacg
     4501 acttcgggga ttaccgggtt gaccttcccg ggcatgatgg atgatccggg ctgcatgggc
     4561 ggaaggttga tctcaaagat cccggttcgc ggc
//
LOCUS       DAOOYG010000177         4380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_23466_length_4380_cov_5.626821, whole genome shotgun sequence.
ACCESSION   DAOOYG010000177 DAOOYG010000000
VERSION     DAOOYG010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23466_length_4380_cov_5.626821"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /gene="murD"
                     /locus_tag="PK288_11015"
     CDS             complement(<1..1058)
                     /gene="murD"
                     /locus_tag="PK288_11015"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPM19500.1"
                     /translation="MARIVILGAGESGTGSAVLALKKGYDVFVSDMGRVKQNYRELLN
                     KYNIRWEEGGHSEDEILTADEVIKSPGIPEDAPIVKLIRGKGIPVISEIEFAGRFAGG
                     KKICVTGSNGKTTVTSLIYHILKKAGKNAAMTGNVGNSFAMAVAEGDYDYYVIELSSF
                     QLDGMFSFRADIAILMNITPDHLDRYGYDMKNYVDSKFRVVQNLTPSEFFIFWADDPV
                     IKTELQKRNVPGVLLPFSAVSKEGMAAYIENDELVIDYPHKTNLMTIHDLALKGRHNI
                     FNSMAAAIAGKVLTIRKEVIRESLTDFQGVEHRMEPVITVYGINFVNDSKGTNVNSTW
                     YALECMETDVIWIAGGID"
     gene            complement(1062..2318)
                     /gene="mraY"
                     /locus_tag="PK288_11020"
     CDS             complement(1062..2318)
                     /gene="mraY"
                     /locus_tag="PK288_11020"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPM19501.1"
                     /translation="MLYYLFRYLHEFNVPGAGMFAYISFRSAMAVISSLIISLLIGRR
                     IIRFLQIKQVGEVVRELGLEGQYQKKGTPSMGGVIILASIIVPTLLFARLDNIYVILM
                     MVTTIWLGFIGFMDDYIKIFRKNKKGLAARSKIAGQIVLGMVVGVTLFFSDDVVILEK
                     VSLNELSERERIELLDASSPFTGAGQFSMERKSTKTTIPFVKNNEFNYAWLVSFIPAE
                     HRRFWTWLVFVAIVIFIVTAVSNGANLTDGLDGLATGVSAIIGTALGILAYVSSNVIY
                     ANYLNIMYIPHSEELVIFAASLIGATIGFLWYNSYPAQVFMGDTGSLALGGIIAVFAI
                     VIRKELLIPVLCGIFLAENLSVVIQVSHFRRTRRKYGEGRRVFLMAPLHHHYQKKGYT
                     EPKIVTRFWIVAIMLAVISIVTLKIR"
     gene            complement(2323..3789)
                     /locus_tag="PK288_11025"
     CDS             complement(2323..3789)
                     /locus_tag="PK288_11025"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPM19502.1"
                     /translation="MATLSELVKGVQVISVSGDVNRKIEGVAFDSRKAGPGYLFVAVK
                     GTKTDGHEYIKPAVESGAVAIVCEKLPSGAGPSVIWITVADSAEALGMICSNFYGNPS
                     AHLHLVGVTGTNGKTTIATLLYRMFSRLGYKCGLFSTVCNYIGEKELPATHTTPDPVQ
                     LNRTLAMMAEEGCGYAFMEVSSHAADQKRIAGLSFSGGIFTNLTHDHLDYHGTFSNYL
                     KAKKSFFDSLADNAFALVNTDDKNGRVMLQNCRARKYTYSVRGMADFRAGIIEQDFSG
                     MELRIQGEEVWTRFIGDFNASNLLAVYACSELLGAEKTEILTILSDLGPVPGRLESVR
                     SGSGITGLVDYAHTPDALFNVIETINRIRGKGSRLLTVVGAGGDRDRSKRPGMASISA
                     GGSSKVILTSDNPRTEDPERILDDMEAGITPEQRPGVLRIADRREAIRTAVMLAQKGD
                     IILVAGKGHETYQEIMGVRHHFDDREELEAALLLKTWN"
     gene            complement(3796..>4380)
                     /locus_tag="PK288_11030"
     CDS             complement(3796..>4380)
                     /locus_tag="PK288_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPM19503.1"
                     /translation="TGTAQIALDKRGYRQGSKISYQASFVGYFPADNPLYSCIVVVNA
                     PSNGVYYGNIVAGTVFREIADKVYATTFFRDYSRESTKKELTASVPEVGNGYRSDINK
                     VLGSLGVRLNKATRDDWVATRESGDTIRLVGLEIKNGLVPDVRGMSLRDAIFLLENAG
                     LRVRYNGMGKVVRQSPEHGARASEGSVVFLDMNM"
BASE COUNT         1073 a         1193 c          928 g         1186 t
ORIGIN      
        1 ttatctattc ctcccgcaat ccagataaca tccgtttcca tgcattcgag cgcataccat
       61 gtggagttca cgttggtgcc tttcgaatcg ttcacgaagt tgatcccgta aacagtgatt
      121 accggctcca tgcgatgttc cactccctgg aaatctgtca ggctttccct gatcacttcc
      181 tttcggatgg tcagtacctt gccggcaatg gcagctgcca ttgaattgaa gatgttgtgc
      241 cggcctttca atgccagatc atgaatggtc ataaggttgg ttttatgtgg gtaatcaatt
      301 acaagttcat cgttctcaat atatgccgcc ataccttcct tcgaaacagc cgagaaaggc
      361 agcagcacac cgggcacatt ccttttctga agctctgtct ttattacggg gtcatcagcc
      421 cagaaaatga aaaactccga cggggtcagg ttttgcacaa ccctgaattt tgagtcaaca
      481 tagtttttca tatcatatcc gtaccggtcg agatgatcgg gggtaatgtt catcaggatg
      541 gcaatatccg ccctgaatga aaacatgccg tcgagctgga agctgctcag ttctattaca
      601 taataatcat aatcaccttc ggcaaccgcc attgcaaagc tgttgcccac attaccggtc
      661 attgcggcat tctttccggc ttttttaaga atatggtaaa tcagactggt cacagttgtt
      721 ttgccgttgc tgcccgtcac gcatatcttc tttcccccgg caaacctgcc tgcaaattca
      781 atctctgaaa taacaggtat gcccttgccg cggataagtt tcactatagg tgcatcttca
      841 gggattccgg gacttttaat cacctcatca gcggtaagaa tctcatcctc cgaatgaccg
      901 ccttcctccc atctgatatt gtacttgttc agcagttccc tgtaattttg ctttacccgg
      961 cccatgtccg agacaaaaac atcatatccc tttttcaggg ccagtaccgc tgagcccgtt
     1021 ccgctttcac ctgctcccag aattactatc cttgccatga cttatcttat cttaagcgtc
     1081 actatactga ttacggccag cattatcgcg acaatccaga acctggtaac gattttgggt
     1141 tccgtatagc cttttttctg gtaatggtga tgaaggggtg ccatcaggaa gactctccgc
     1201 ccttctccgt atttcctcct ggttcttctg aaatgcgata cctggatcac caccgagaga
     1261 ttctcggcga gaaaaatgcc acaaagcacg ggtatcagga gttctttcct gataacaatt
     1321 gcaaaaaccg cgataattcc acccagggcc aggcttcccg tatcgcccat gaaaacctgt
     1381 gccgggtatg agttgtacca caaaaaaccg attgttgctc cgatcaggga cgctgcaaag
     1441 atcacgagct cttcggaatg tggtatatac attatattca gatagtttgc gtaaataaca
     1501 ttactggaaa cgtaggccag gatacccagt gcggtaccta taatcgccga aacacccgtc
     1561 gcaagcccat cgagtccgtc agtaagattg gccccgtttg ataccgcggt aacgatgaaa
     1621 atcacaattg ccacgaaaac aagccatgtc cagaaacggc ggtgttctgc gggaatgaaa
     1681 gataccagcc atgcatagtt gaattcgttg ttcttgacaa aaggaatcgt tgttttggtg
     1741 gatttacgct ccattgaaaa ctgtcctgcc ccggtgaacg gggatgatgc gtcaagcaat
     1801 tcaatccttt ccctctccga aagttcgttc agggagactt tttcgagaat aaccacatca
     1861 tcgctgaaga agagcgtaac cccgacaacc atgccaagaa caatctgtcc ggcaatctta
     1921 gaccgtgctg caagtccttt cttgttcttc ctgaaaattt tgatataatc gtccatgaat
     1981 ccgatgaacc ccagccaaat ggttgtgacc atcatcagta tcacgtaaat attatcgagc
     2041 ctggcaaaga gcagggtcgg aacaattatg gaagcaagga ttatcacgcc tcccattgac
     2101 ggggttccct ttttctgata ctggccttcc aggccaagtt ccctgaccac ctcccccacc
     2161 tgctttatct gcaggaaacg gatgatcctc ctgccaatga gcagggagat gatcagcgat
     2221 gaaataaccg ccattgccga cctgaaggag atgtatgcaa acattcctgc gccgggcaca
     2281 ttaaattcat gcaggtatct gaataagtag tagagcattt ccctaattcc aggttttcaa
     2341 caataatgca gcctcaagtt cttccctgtc gtcaaaatga tgcctgacac ccataatctc
     2401 ctgataggtt tcatgacctt ttcctgccac aagaattatg tcccccttct gtgcaagcat
     2461 cactgcggtt ctgatggctt ccctcctgtc ggcgatcctg agtaccccgg gccgctgctc
     2521 aggagttatt ccagcttcca tatcatcaag tatccgttcc ggatcctctg tcctcggatt
     2581 gtcggaagtg agaatgactt tagaacttcc tcctgccgaa atggatgcca ttccgggtct
     2641 ttttgacctg tctctgtcac cgcccgcccc gacaactgtc agcagccggc tccctttccc
     2701 ccttatcctg ttaatcgttt caataacatt gaacagggcg tcgggtgtat gggcataatc
     2761 gaccagtccg gtaatcccgc tgccggacct gacggattca aggcggccgg gtaccggccc
     2821 gagatcactc aggatggtga gtatctctgt cttctctgct cccagcaatt cagagcaggc
     2881 ataaacagca agaagattcg atgcattgaa atctccgata aatcgtgtcc agacctcttc
     2941 accctggatc ctgagctcca tgcccgaaaa atcctgttca attataccgg ccctgaagtc
     3001 ggccattccc cttactgagt atgtatattt tcgggctctg caattctgca gcatcaccct
     3061 tccattttta tcatctgtat ttacaagggc aaatgcatta tccgccagtg aatcgaagaa
     3121 gcttttcttg gctttgaggt aattactgaa cgtgccgtgg taatcaaggt gatcgtgtgt
     3181 caggttggta aaaatcccgc ctgaaaagct gagtccggca atgcgcttct gatcggctgc
     3241 atgcgagctt acctccataa aggcatatcc gcagccttct tccgccatca tggccagggt
     3301 ccggttcagc tgaaccggat caggcgtggt atgcgttgcc ggaagttcct tttcgcctat
     3361 atagttgcaa actgttgaga aaagaccgca tttgtatccg agtctggaga acatccggta
     3421 gagaagtgtt gctattgttg ttttcccgtt tgttccggtg actccgacaa ggtgcagatg
     3481 agccgatgga ttcccgtaaa agtttgagca gatcattcca agggcctctg cagaatctgc
     3541 gacagttatc cagatgacag aaggaccggc accggaaggg agcttttcgc agactatggc
     3601 gacagcccct gattccacag ccggtttaat atattcatgc ccgtcggttt tcgttccttt
     3661 aaccgccaca aaaaggtatc ccgggcccgc tttccgggaa tcaaatgcca cgccttcaat
     3721 cttccggttc acatctcccg aaacagagat aacctgcacc cctttcacca attcactcag
     3781 tgtagccatt cttccttaca tattcatatc aagaaataca acactccctt cacttgccct
     3841 cgcgccatgt tcgggagact gcctgacaac ctttcccatg ccgttgtatc ggactctcag
     3901 tcccgcattt tcaagcagaa aaatcgcatc acgcaggctc atccccctta catcgggaac
     3961 aagtccattt ttaatttcca atcctacaag tctgattgta tcaccgcttt cacgggttgc
     4021 cacccagtca tcgcgcgtgg ctttatttaa cctgacccct aagcttccga gaaccttgtt
     4081 tatatcgctc ctgtaaccat ttccgacttc cggaacagac gctgtcagct ccttttttgt
     4141 actttcccgg ctgtaatccc tgaaaaatgt cgtggcataa accttatcgg caatttccct
     4201 gaacactgtc cccgcaacta tattgccgta ataaactccg tttgacggtg catttacaac
     4261 cacaatacag gaatacagcg gattatcagc cggaaaatag ccaacaaacg aagcctgata
     4321 gctgatcttc gagccctgcc gatatcctct tttgtcaagc gctatctgag ctgttcctgt
//
LOCUS       DAOOYG010000178         4347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_23726_length_4347_cov_9.425443, whole genome shotgun sequence.
ACCESSION   DAOOYG010000178 DAOOYG010000000
VERSION     DAOOYG010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23726_length_4347_cov_9.425443"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            614..1294
                     /gene="trmD"
                     /locus_tag="PK288_11035"
     CDS             614..1294
                     /gene="trmD"
                     /locus_tag="PK288_11035"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPM19504.1"
                     /translation="MRIDILTVCPELLESPLNYSIVKRARDKGLVEINVINLHDFARD
                     RYKSVDDYAFGGGAGMVMMPEPVFRATEMLKSEREYDEIIYTSPDGIRFNQKMANRLS
                     LKNNIIILAGHYKGIDQRIRDHLITVEISVGDYVLSGGELPAAIITDAIVRILPGAIS
                     DEQSALSDSFQDDLLAPPVYTRPADFRGWKVPDILLSGNTAEIDKWRHEQSLLKTQKL
                     RPDLTQNE"
     gene            1287..>4347
                     /locus_tag="PK288_11040"
     CDS             1287..>4347
                     /locus_tag="PK288_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010801924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPM19505.1"
                     /translation="MNRMLSSLKGFMAFAIMFLAISCDNNFHKDYLKDGFINPPDSAR
                     PGVYWYFMDGNIERKAITADLESMKEEGIGYVVFLEVNVGVPRGKIDFLSEEWQELYR
                     HAVREAERLGIRIVLGSGPGWAGSGGPWVTPARSMLHLVAADTVVNGPALFNGILAVP
                     EPKRPFFGENSLTPELKKLRDEWYNDIYVLAFPTPRNPERIKLIDEKANYYRAPYTSQ
                     AGVLPYISPSDTRSRITGSAIDLKKIIDISEYMDAGGRLKWDVPPGNWTIMRFCTRNN
                     GAVTRPAPLPGLGFECDKFDTSAFNSHYEAYAGKLIRKSQPVKTSSGGGWTMIHIDSW
                     EMGAQNWSPHFREEFIERRGYDPKPFLPVYSGLVVDNTEISERFLWDIRQTSSELIIE
                     NHALHFKKLGRRSGFRLSVEPYDMNPASDLDLGLTADVPMCEFWSEGYGFNSAFSCIE
                     STSVAHISGAPVVAAEAFTAGNDEAWKQYPGSMKNQGDWAFCLGINRFFYHTFAHKPY
                     DEKYRPGMTMGPYGVHWDRGQTWWPMADEYHRYIARCQYMLSQGKAVSDILYLAAEGA
                     PHVFRPPSSALDGTQFLPDKKGFSFDGCSPDFLINNAEVRDGMITFPGGGSYRIMVLP
                     DINTMTPELLKKIRSLAESGALIAGNPPERSPSLSGFPDCDRQVAELAGEIWGKHVIP
                     DGLSRHNFGKGKIFWGKRLVNKHKGIRNDPDSLALYPDFALIDSILSASGIIHDFKAS
                     GNIRYTHRSLPDRELYFISNRTGSPVKDTCYFRDGSPDAELWDAVTGEIRPVNIFFND
                     AGIAYAGIELDSYQSYFIVFYKNPRPDKMKSGNTGTSKEKNILTTLNWQWIVDFDTVM
                     GGPGKVSFENLDDWAQNADDGIRFYSGTAIYSKTFDLPESGVSLKNSKVYLNLGKVRN
                     IARVTLNGRDAGTIWTAPWQADITGLLKNRNNVLEIEVANLWINRLIGDEAEPWDGVT
                     EGKWPEWFLNGTKRPTSRYTFTTHRYYKTGDPLSESGLLGPVQIEINR"
BASE COUNT         1244 a          948 c         1031 g         1124 t
ORIGIN      
        1 ctcctcgcaa tgacggtagt tctaccgcca catcgtgtta ccgtcattgc gaggagtccc
       61 gttttgcacg gcaaaacggg acgacgaagc aatccttctc atcgttccag caaccttagt
      121 atccaaactg cctcttcccg cgaattcccg caaaactgaa cttccgcttt gtaatccgaa
      181 caaaccttcg gtctgtaagg attaccccac aaagcacatt taaaatcacc tgtaagatgg
      241 atacatctca ctcctgccgg cttgccgtca gtcatacccg ggatgggtga tgaaatcgat
      301 atttctatgc aacaggctcc gcagccggaa cgacaatcca tgataaaatg ataacagaca
      361 aagataagga ttattatagt ttaatggcgc aacggcgcaa cggcacaacg gggcaacggc
      421 gcaatgtcgc aacggttcaa cagcgttatg gcgttgcggt attgcggtat tatggtcgta
      481 aaaccgtaac accgtaaagc cataacgccg taacgccatt tcgatatttc gttatttcac
      541 tattacgccg ttgcgcctct gtgccgttgc accattcttt tattcttcct acttttacat
      601 aaatatcttc ctgatgagaa ttgatatcct tacagtctgc cccgaactgc tcgaaagtcc
      661 actgaattat tccatcgtaa aacgtgccag ggataaaggc cttgttgaga tcaatgtcat
      721 taaccttcac gattttgcca gggacaggta caaaagtgtc gatgattatg ctttcggagg
      781 aggtgcaggg atggtaatga tgcccgaacc ggtcttcagg gcaacagaga tgctgaaaag
      841 cgaaagggaa tacgatgaaa taatttatac ttcgcccgat ggtataagat tcaaccagaa
      901 aatggctaac aggctctctc tgaaaaacaa tataatcata cttgccggac attataaggg
      961 aattgaccag cgtatcaggg accatcttat cactgtggag atatcagtgg gtgattatgt
     1021 tctttccggc ggggaactac ctgctgcgat cataacagat gctatcgtca ggatccttcc
     1081 tggtgctatc tccgatgagc aatcggcatt gtcagactca ttccaggatg atctccttgc
     1141 ccctcccgtc tataccagac ctgccgattt caggggatgg aaggtgcctg atatactcct
     1201 ctcaggcaac actgctgaaa tagataaatg gcggcatgaa cagtctttac ttaaaacaca
     1261 aaaactacga cctgatctga ctcaaaatga atagaatgtt atcttccctt aaaggattca
     1321 tggcttttgc tatcatgttc ctggcaattt catgcgataa caatttccac aaagattatc
     1381 tgaaagatgg atttataaat cctccggatt ctgcccgacc tggcgtttac tggtatttta
     1441 tggatggtaa catagaacgt aaggcaataa ctgccgatct ggaatcgatg aaagaggaag
     1501 gtataggcta tgtagtattt ctggaggtca atgtaggagt gccgaggggt aagattgatt
     1561 tcctgagcga agaatggcag gaactttacc ggcatgcagt tagagaagct gaaaggcttg
     1621 gcatcaggat cgttctggga tcgggtccgg gatgggcagg aagcggtggt ccctgggtca
     1681 ctccggcccg gtcaatgctt catctcgtgg cagccgatac tgttgtcaat ggtcccgcat
     1741 tgtttaacgg catccttgcg gtgcctgaac cgaaaaggcc tttcttcgga gaaaacagcc
     1801 ttacccctga acttaagaag ttgagggatg aatggtacaa cgatatttac gtactcgctt
     1861 ttccgacgcc ccgcaatcct gaaagaataa aactgatcga tgaaaaagcc aattattaca
     1921 gagctccgta tacatcacag gccggggtgc tgccatatat ttctccttca gatacccgga
     1981 gtagaataac cggatctgct attgacctga aaaaaataat tgatatttca gaatatatgg
     2041 atgccggcgg caggcttaaa tgggatgtac ctccgggaaa ctggacaata atgagattct
     2101 gtacaaggaa taatggcgcg gttacacgtc ctgcaccttt acccggactg ggttttgaat
     2161 gtgataagtt tgacacttct gcttttaact ctcattatga agcttatgca gggaagctta
     2221 tcaggaaatc acaacctgta aaaacctcat ctggaggagg ctggacaatg atccacatcg
     2281 acagctggga aatgggagct cagaactgga gcccgcactt cagggaggaa tttatagaac
     2341 ggcgcggtta tgaccccaaa ccctttcttc cggtttattc aggactggtc gtcgataata
     2401 cagaaatctc tgaaagattt ctctgggata ttcgtcagac atcttccgaa ttaataattg
     2461 agaatcatgc acttcatttt aagaaactcg gaagaagaag cggattcaga ctctctgtag
     2521 aaccatatga tatgaatcca gcttccgacc ttgatcttgg tctgactgca gatgttccga
     2581 tgtgtgaatt ctggagtgaa ggctacggat ttaactcggc attcagctgt atagagagta
     2641 cttctgtcgc tcatatatca ggagcacctg tcgtggctgc cgaagctttt actgccggaa
     2701 atgatgaagc ctggaaacag tatcccggaa gtatgaaaaa ccagggtgac tgggctttct
     2761 gtctgggaat caacagattc ttttatcata cattcgctca taagccttat gacgaaaaat
     2821 acaggcccgg aatgacaatg gggccctatg gggttcactg ggacaggggt cagacctggt
     2881 ggccaatggc tgatgaatat cacaggtata tcgcacgttg ccagtatatg ttgtcccagg
     2941 gaaaagcggt atcagatatt ctgtaccttg ccgcagaagg agctcctcat gtattccggc
     3001 caccatcctc cgctctggat ggcacacaat tcctgcctga taagaagggc ttttcatttg
     3061 acggatgctc tccggatttc cttataaata atgctgaagt cagggacggt atgataacat
     3121 ttcccggtgg tggctcatac aggataatgg ttttaccgga tataaataca atgacacccg
     3181 aacttcttaa aaagatcaga tcacttgctg aatccggtgc actgatcgca gggaatcccc
     3241 cggaaagatc tccttctctg tcaggttttc cggattgtga cagacaggtc gcagaattag
     3301 ccggtgaaat atggggaaag catgtgattc ctgatggatt atcacgacat aatttcggga
     3361 aagggaaaat attctgggga aaaaggctgg ttaacaaaca taaggggata agaaatgatc
     3421 cggattcact tgcactctat cccgactttg cactaattga ttctatcctg tcagcttcgg
     3481 ggataattca tgatttcaag gcatccggca acatacggta tacccacagg tcgcttcctg
     3541 acagggagtt gtatttcatt tccaacagga ctgggtctcc tgtaaaagat acatgttact
     3601 tccgggacgg aagtcctgat gccgaattat gggatgcagt aaccggagaa atcagaccag
     3661 ttaatatttt ctttaatgat gccggaattg catacgcagg gattgagctg gattcatacc
     3721 agagttactt tatcgtcttc tataaaaatc cgcgaccgga taaaatgaaa tccggcaata
     3781 ccggaacaag caaagagaag aatattctca cgacactgaa ctggcaatgg attgttgatt
     3841 tcgatactgt aatgggggga cccgggaagg taagtttcga aaacctggat gactgggctc
     3901 agaatgccga tgacgggatc agattttact caggaacagc catttattca aaaacctttg
     3961 atctgcctga atccggagta tcattgaaaa attctaaagt atatctcaat ctgggaaagg
     4021 taaggaatat cgccagggtc acacttaatg gccgggatgc cggtacgatc tggaccgcac
     4081 catggcaggc tgatatcaca ggtctgttaa aaaaccgaaa taatgttctt gaaatagagg
     4141 ttgccaattt atggataaac aggcttatag gtgatgaagc cgaaccatgg gacggagtaa
     4201 cagagggcaa atggcccgaa tggtttttga atgggacaaa aaggccaaca agcaggtata
     4261 cgttcacaac acacaggtat tataaaacag gtgatcctct gtcagaatca ggattattgg
     4321 gccctgtaca gattgaaatt aaccggt
//
LOCUS       DAOOYG010000179         4264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_24385_length_4264_cov_5.376336, whole genome shotgun sequence.
ACCESSION   DAOOYG010000179 DAOOYG010000000
VERSION     DAOOYG010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24385_length_4264_cov_5.376336"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK288_11045"
     CDS             <1..456
                     /locus_tag="PK288_11045"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPM19506.1"
                     /translation="AMVSAEAHVVTPAFEHVVEASILLSGLGFESGGLAASHAIHNGL
                     TVLKETHHLFHGEKVAFGVLATLFLTDRPADIIDDVYSLCLAVGLPVTFEELGLKNPS
                     DEDLLRVGEASCAEGETIHNEAGEITPKAVVAALRAADGEGRRRKKLKN"
     gene            834..1421
                     /locus_tag="PK288_11050"
     CDS             834..1421
                     /locus_tag="PK288_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPM19507.1"
                     /translation="MKSKVFITVVLLAGFPLGLIAGGGKARASLEINGGLSLSVCSPG
                     GSVTNAGAGGEALFHYRFAKHLGIYCGWGYNAFRNDYAPAGYRCDFEETGYSVGLQYK
                     RRIEFSNSSFFIRGGFQYKHTEIEDPSGELLFDTTHGPGWQAGCGVDLPLAEKWSLLP
                     EVRFNSLPEKEGHTTTSPIPDRNYFSVRLGIIRRF"
     gene            1442..1819
                     /locus_tag="PK288_11055"
     CDS             1442..1819
                     /locus_tag="PK288_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19508.1"
                     /translation="MMNFREIVLIMSAVAITILNTCEPMAAGRYGFDLPVGPGSEGKN
                     TVTVDFTVSAIHLTGIMRVSKGTLSAELMSPSGAVVFSAAVEAPGEFKIDRYYPAGCG
                     AWKMRYYSTSGTGYLRMQLNLIR"
     gene            1907..3001
                     /locus_tag="PK288_11060"
     CDS             1907..3001
                     /locus_tag="PK288_11060"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPM19509.1"
                     /translation="MAINLIRRLNRLIPAGRLIRFIIGISVTVQLIVIVYNNLSGFYP
                     LDNFNHFLLRLLRGVLLTVPAATLIIVPDLLLIDWLNRELPWNRKVMERVLIQFPASL
                     ALAVAVSVLMTLTANTISAYTEPLKLVFLYNALVFAVVNIIVAGVLEAWVSYNRGAEA
                     AKKAEMLERELTQIRFEMLRSQINPHFMFNSLNVLSGLIEKDVARAQEFIDEFSRIYR
                     YVLETVEQPATTLGRELEFARSYFYLQQMRYGENLSWTINLTAGMLGLLLPPLSLQVV
                     LENAIKHNIVNQSKPLHIDIGSGDMVLVVSNRLQPKISATASTGVGLKNLTRRYSLIS
                     NRIPRFTVENDHYIARLPLVNPESDESTDN"
     gene            2982..3737
                     /locus_tag="PK288_11065"
     CDS             2982..3737
                     /locus_tag="PK288_11065"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HPM19510.1"
                     /translation="MKALIIEDESVAADKLEKMLMDIDPAVNVVNKTGSIRDSVRWLM
                     NNQADIIFLDIQLSDGISFSIFEQVAVNTPVIFTTAYDQYAVKAFRLNSIAYLLKPIR
                     REDLAESLRKYHTLRTAFSIDFDSLLAQVQGRDPGYRKRFLIQTGEKIRKIEVTDIAW
                     FHALGKGVYMKTSQGQTFPSEFSLEGLEKILDPAIFFRVNRKYLVNIDAIAGMTAWSR
                     GRIRLELKPKADNELDTVVSIDRAAAFRKWLNA"
     gene            3794..>4264
                     /locus_tag="PK288_11070"
     CDS             3794..>4264
                     /locus_tag="PK288_11070"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HPM19511.1"
                     /translation="MQKMASLLTGQLINYSDLVREAGVTAATAQRFVNYLDMSYQTLQ
                     LQPWTRNKVKRLTKSAKLHYLDPGIQRAIIGKRGGMSGNEYESAIVAEIFKQARNLNF
                     SCTFYHLRTVDGREIDLLIETEKGYIAIEIKMSGRIERTDARHIDGLSGILDKPL"
BASE COUNT         1127 a          988 c         1084 g         1065 t
ORIGIN      
        1 gccatggtat cggcagaggc tcacgtggtg actccggcat ttgaacatgt cgtggaagcc
       61 tccatcctgt tgagcggcct gggtttcgag agcgggggcc ttgctgctag tcatgccata
      121 cacaacgggc tcacggtttt aaaagagact catcatcttt tccatggtga aaaggtggcc
      181 ttcggtgtgc ttgcaacctt gttccttaca gatcgccctg ccgatattat tgatgacgtc
      241 tattcccttt gccttgcagt tggcctgcct gttacttttg aagagctggg actaaaaaac
      301 ccttcggatg aagacctttt acgcgtgggt gaagcttcct gcgccgaggg agaaacaatt
      361 cacaatgaag ccggtgaaat aaccccgaaa gcagttgtgg cagccttaag ggcagctgac
      421 ggggaaggac ggcgaagaaa aaaactaaaa aactgacctt aaaccactgc gggtttacct
      481 attgttgctg ctcctttttg gggctgagaa atatattgaa tgattaattg cctggatcat
      541 cccggagccc caggttccgg gaacaggcgc ttcacgcgcc ggacctgtaa tatttcagga
      601 tgcaatcata attttagccc tcccgggcta ttacacatga acatgcaggt gctgttcagc
      661 atgaataata tcttccactt ctttgcctgg ttgggttcag gcctttcata ccctgaataa
      721 acaccttcag cggccttttt cacgcccctg tcatcttttc ttttctaccc cgggtagctg
      781 cttttacttt tgcttcgaca aaaaaaaata cgaatcctta aaagcaattg aaaatgaagt
      841 caaaagtgtt tattacagta gttcttttag ctggctttcc cctggggctc attgcagggg
      901 gtgggaaagc aagggcttcc ctggagataa acggggggct ctccctgtcg gtttgttctc
      961 ccggcggatc cgtgacaaat gcaggcgcag gcggtgaggc actctttcat taccgttttg
     1021 cgaaacacct cggcatttac tgcggctggg gttacaacgc cttcaggaat gattatgcac
     1081 cagccgggta ccggtgcgat ttcgaagaaa cgggttacag tgtcggactt caatacaagc
     1141 gccggattga attctcaaac agctcgttct ttataagagg aggcttccaa tacaagcata
     1201 cagagatcga agacccgtca ggcgaactgc ttttcgacac cacacacgga cccggctggc
     1261 aggcaggatg cggtgttgat cttccgctgg cagaaaaatg gtctctcctc cccgaggtca
     1321 ggttcaattc ccttcctgaa aaggaaggcc atacaaccac ctcaccaatc cccgacagga
     1381 attacttctc cgtcaggctt ggcatcatca gaagattctg aatcagttct cagtataaaa
     1441 aatgatgaat ttcagggaaa tagttctgat aatgtcggca gttgcaatta caatcctgaa
     1501 cacctgtgag ccgatggcag ccggccggta tggttttgat ctgccggtcg ggcccggctc
     1561 tgaagggaaa aacacggtta cagttgattt cacagtcagt gccatacatc tgacaggaat
     1621 aatgagagta tcgaaaggca cattgtcggc agagttgatg tcaccatcgg gggcggtggt
     1681 ctttagtgct gccgtcgaag cccccgggga gtttaagatc gacaggtact atccggccgg
     1741 ctgcggcgcc tggaaaatgc gctattacag caccagcggt accgggtatc tgaggatgca
     1801 gctgaatctg atcagataag acagtaatca tgacagggat acaggttttc agttattttt
     1861 gctattcggg aatcagctta ccggaatatc gggtaaatat accagcatgg cgataaactt
     1921 aatacggcgg ttaaacaggc tcatccctgc agggaggctg atcaggttca tcatcgggat
     1981 atcagtgacg gtacagctga tcgtgattgt ttataacaat ctcagcggat tctaccctct
     2041 tgataacttc aatcatttcc tgctcaggct ccttcgagga gtgctcctca cggtgcctgc
     2101 agcgactttg ataatcgtcc ccgacctttt gttaattgac tggcttaaca gggaacttcc
     2161 ctggaaccgg aaagtaatgg aaagggtact gatccagttt cctgcatcac ttgcccttgc
     2221 tgttgcagtt tcagtgctta tgactctgac cgccaatacc ataagtgctt acacggagcc
     2281 tttgaaactt gtgtttctct ataatgccct cgtctttgct gtcgtgaaca ttatagtggc
     2341 aggggttctt gaagcctggg tttcttacaa ccggggagcc gaggcagcca aaaaggctga
     2401 aatgcttgaa cgcgagctga cacagattcg attcgagatg ctgagaagcc agatcaaccc
     2461 ccatttcatg tttaacagcc tgaacgtgct gtcggggttg atcgaaaaag atgtcgccag
     2521 ggcacaggag ttcattgacg agttctcccg gatttaccgt tatgttctcg agacggttga
     2581 acaacccgcg acaaccctcg gaagggaact ggaattcgcg cgttcatatt tttatcttca
     2641 gcagatgcgc tatggagaaa acctttcatg gacaattaat ttgactgcag gaatgctggg
     2701 gctattgcta cccccgctgt cactgcaggt agtcctcgag aatgccatta agcataatat
     2761 cgtgaaccag tcaaaaccat tgcatataga tataggaagc ggcgacatgg tgctggtagt
     2821 gtcaaacagg cttcaaccta aaatttcagc aaccgcgtca accggggtgg ggctcaaaaa
     2881 tctcacgagg cgttactcgt tgataagcaa tcggatacct cgttttacgg ttgagaacga
     2941 tcattatata gctagattgc cacttgtaaa tcctgaaagc gatgaaagca ctgataattg
     3001 aagacgaaag cgttgctgct gataagctgg agaaaatgct catggacata gatcccgcgg
     3061 taaacgttgt aaataaaacc ggatcgatca gggattcagt gagatggctt atgaataacc
     3121 aggctgatat aatcttcctg gatatacagc tttcagacgg tatcagcttc agcatcttcg
     3181 agcaggtggc agtcaatact ccggtaatat ttaccacggc atatgaccag tacgcggtga
     3241 aggcattcag gcttaacagc atcgcttacc tgctcaaacc catccggcgt gaagacctgg
     3301 ccgaaagttt aagaaagtac catacactca ggacagcctt cagcatcgac tttgattccc
     3361 tcctcgctca ggtacaggga agggatccgg gttacaggaa gcgcttcctg atacagacag
     3421 gtgaaaagat caggaagata gaggtgacgg atatagcatg gttccacgcc ctcggaaaag
     3481 gagtatatat gaagacttcc cagggacaga cattcccttc ggaattcagc cttgaagggc
     3541 tggagaagat actggaccca gcgatttttt tcagggttaa caggaaatac ctggtgaata
     3601 tagatgccat cgccgggatg acagcatggt cgcgggggag aatcaggctg gaactcaagc
     3661 caaaagctga taatgaactt gataccgtgg tgagcatcga cagggcagct gccttcagaa
     3721 agtggctgaa cgcctgactt ctggtcccgg ctcccagcgg gtgcggttga aatacccgga
     3781 acctttcgtc agtatgcaga agatggcatc cctccttacc ggacaactga ttaattattc
     3841 cgatctggta agggaagccg gagttacggc ggcaactgca cagagatttg taaactacct
     3901 tgacatgagc tatcagacac ttcagcttca gccatggaca aggaacaagg tgaaacggct
     3961 tacaaaaagt gcaaagcttc attacctcga tcctggcatt cagagggcaa taatcgggaa
     4021 aaggggcgga atgtcgggaa atgagtatga gagcgctatc gtggcggaaa ttttcaagca
     4081 ggctcggaat ttaaattttt cttgcacatt ttatcacctt cgtactgttg atggcaggga
     4141 aatcgatctt ctaattgaaa cggaaaaagg ttatattgct atcgagataa aaatgtcggg
     4201 aagaattgaa aggacagatg ccaggcatat tgatggtctt tccggtatac ttgacaaacc
     4261 gctc
//
LOCUS       DAOOYG010000180         4263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_24392_length_4263_cov_7.038498, whole genome shotgun sequence.
ACCESSION   DAOOYG010000180 DAOOYG010000000
VERSION     DAOOYG010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24392_length_4263_cov_7.038498"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1420
                     /locus_tag="PK288_11075"
     CDS             74..1420
                     /locus_tag="PK288_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HPM19512.1"
                     /translation="MVRKNVVIIGAAGRDFHNFNICFRDNESYNVVAFTAAQIPDIDG
                     RRYPADLAGKLYPKGIPVFAEENLPEIIRKHKVDQCIFSYSDVTYNKVMSVGAIVNSA
                     GADFVLLGPDATMVKSTKPLIAVGAVRTGCGKSQTSRKVIEILMAKGLKVVAVRHPMP
                     YGNLSEQKVQRFATLKDLEKHKCTVEEMEEYEPHVVRGNVIYAGVDYEAILRKAEKDP
                     AGCDVVVWDGGNNDFPFYKPDLMITVTDPHRAGHELRYYPGEVTLRIADVVVINKIDS
                     ADPDSINIVRESIEKVNPKATVIDAASPIKVDDPSVIRGKNVLVVEDGPTLTHGEMKL
                     GAGIMAARKFGAAGIIDPRPFVVGKLKETFGLYPGIGTLLPAMGYGEQQLRDLEKSIN
                     NTKCDSVIVATPIDLQRIINIEKPATRVNYELQEIGDPTLSQILDEFVAKNKLRKK"
     gene            complement(1467..2546)
                     /locus_tag="PK288_11080"
     CDS             complement(1467..2546)
                     /locus_tag="PK288_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPM19513.1"
                     /translation="MKIELKKDFTWSLVVPTSMGVRITPLNGQPVHCSDTFHMQATSA
                     ETNVASVSSYLGLPVKVLTTFVRESPVARFIKDNLAGRHMAYEGAEVDQGGPWGYRHQ
                     FNIADSGYGSRGPRVQNDRAGEVGRTLNVKDFDLGRLFDKEGVQILHLSGLIGALSPE
                     TSNFCLELARAARKYGTRISFDLNYRATFWKNREKELSATFKEIAGLADILVGNEEDF
                     QLCLGIEGPEEGGKDLASKIGSFKEMIGRVKKAYPGTSVFATTLRQVISTNEHLWGAI
                     MLEGDNWHIVEPREIHVLDRIGGGDGFVGGLLYAILKGWDAEKWIQFGWATGALATTV
                     ITDYGQPADEDQVWSIWKGNARVKR"
     gene            complement(2781..4127)
                     /locus_tag="PK288_11085"
     CDS             complement(2781..4127)
                     /locus_tag="PK288_11085"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPM19514.1"
                     /translation="MEKNISIDLKNAAGFISYEEVVALAQQSIRHLDSLNAGTGAGND
                     FLGWLSLPDDIVKQFDRIEKTAKHLRSVSDTTVVIGIGGSYLGAKAVIEGLSHSFSPL
                     LRSKHHEIIFAGQNICEDYMSDLIEILDGRNYSIIVISKSGTTTEPAIAFRILKDHLE
                     RRVGKARAADMIIAITDSSKGALRSLADLNGYDTFIIPDDIGGRYSVLTPVGLLPIAV
                     AGADIRMLVRGAHEMALITRKNKDAVSNPSLMYAAARNLLLQTGRPVETLVGFTPRLH
                     YFSEWWKQLFGESEGKEGKGIFPASVDFTTDLHSLGQYIQEGQRILFETFLSVAGTRK
                     KVTIPPEKDDSDQLNYLAGKRLSEVNNNAETGTILAHNDGNVPVIKINIPELNEFFIG
                     QLIYFFEMACAISGYILDVNPFDQPGVEAYKKNMFALLNKPGFEEAGKKLRERLGR"
BASE COUNT         1161 a          980 c          961 g         1161 t
ORIGIN      
        1 caaattatac atttgctgat tcattttaaa atgaaaatct gtactgtcaa taaattgctg
       61 aaaaaataaa tttatggtaa gaaaaaatgt agtaattatc ggtgctgccg gaagggattt
      121 ccacaacttt aatatttgtt tcagggataa tgaaagctat aatgttgttg ctttcacagc
      181 agcccagata cccgatattg acgggagaag gtatcctgcc gaccttgccg ggaaattata
      241 tcctaaaggc attccggtct ttgcagagga gaaccttcct gaaataataa ggaaacacaa
      301 ggttgatcaa tgcatttttt cctacagtga tgttacatat aataaggtga tgtcggtcgg
      361 tgccatcgtt aattccgccg gtgccgattt cgttctgctt ggtccggatg caacaatggt
      421 aaaaagtacg aagcccctta ttgcagtggg agcggtgagg acaggttgtg gaaagagcca
      481 gacctcgagg aaagtgattg agatccttat ggcaaaaggg ctgaaggttg ttgctgtccg
      541 tcatcccatg ccatacggta atctgagtga acagaaggtt cagaggtttg ccactcttaa
      601 agatctcgaa aagcataaat gtactgtaga ggagatggag gaatatgaac ctcatgttgt
      661 acggggtaat gttatttatg ccggggttga ttatgaggct attctccgga aagctgaaaa
      721 ggatcctgca ggatgcgatg tcgttgtctg ggatggaggg aataatgatt ttccgtttta
      781 taaacctgat cttatgataa ctgtgaccga tccccacagg gccgggcatg agctgaggta
      841 ttacccgggg gaggttactc tccggatcgc tgatgtggtg gttataaaca aaatcgacag
      901 cgctgacccg gactccataa acattgtaag ggaaagtatt gagaaagtca atccaaaggc
      961 aacagtcata gatgctgcat caccaataaa agttgatgat ccttcggtga tcaggggaaa
     1021 gaatgtactc gttgttgaag atgggccaac ccttacccat ggagagatga agcttggcgc
     1081 aggtattatg gctgcccgta agtttggggc tgcagggata attgatcccc ggccatttgt
     1141 tgtgggcaag ctcaaagaaa ccttcggcct ttatcccgga atcggaactc ttctcccggc
     1201 catgggatat ggagaacaac agcttcgtga ccttgaaaag agtatcaata atacaaagtg
     1261 cgacagtgta attgtagcaa cacctatcga cctgcagcgg ataataaaca tagagaaacc
     1321 tgcaacaagg gtgaattatg aattgcagga gattggtgat ccgactctgt cacagatact
     1381 ggatgaattt gttgcaaaga ataaactaag gaaaaaatga ttcctgccgg ggattccggt
     1441 ggcaggaatc ataaatgaaa tattccctat ctttttactc tggcattacc tttccagatg
     1501 ctccaaacct ggtcctcatc tgcaggctgt ccataatcgg tgataaccgt tgttgcaagt
     1561 gctccggtag cccatccgaa ctgaatccat ttttccgcat cccagccctt aaggatggca
     1621 taaagcagtc cgcctacgaa accgtctcca cctccaattc tgtcgaggac atgaatttca
     1681 cgtggttcaa cgatatgcca gttatcacct tcaagcatta ttgcacccca gaggtgctca
     1741 tttgtactga tgacctggcg gagagtggtg gcaaacactg atgtaccggg ataagccttc
     1801 ttcaccctgc ctatcatttc cttgaagctt ccgatcttcg aggcaagatc ttttcctcct
     1861 tcttcgggac cttcgatacc cagacaaagc tggaaatcct cttcattgcc cacaagaata
     1921 tctgccagac cggctatttc cttaaaggtt gcggaaagtt ccttctccct gtttttccag
     1981 aaggttgcac ggtaattaag gtcgaaggag atcctggtac cgtatttcct ggctgctctt
     2041 gccagttcga gacagaaatt gcttgtttcg ggagaaagag cacctataag tcctgaaaga
     2101 tgaaggatct gtactccttc tttgtcaaat aacctcccga gatcgaaatc cttcacattc
     2161 aatgtccttc ctacttcccc ggcacggtcg ttctgaactc tcggtcctct tgatccgtaa
     2221 ccgctgtcgg ctatgttaaa ctggtgcctg taaccccagg gtcctccctg atcgacctca
     2281 gcaccttcat atgccatatg ccggccggcc agattatcct ttataaaacg ggcaaccgga
     2341 ctctccctta caaaggttgt caatactttt acaggtaatc caagataaga acttacactg
     2401 gcaacattgg tttcggcact ggtcgcctgc atgtgaaatg tatcgctgca atgtacaggc
     2461 tgaccgttca acggggtgat ccggacaccc atactcgtgg gcacaaccag tgaccaggta
     2521 aaatcttttt tcaattcaat tttcataata attagtttaa agttctataa aaaatataat
     2581 ttttattctt acttacgacc atacgatcat acgacctgag accgccagcc ttcgccaccc
     2641 cttccgctaa agctttgcag gccgaaaaag gcttcggctg gcaaagcaaa accgtcaagc
     2701 cgtcagaccg ttaaaccgtc aaaccgttaa aaagttaaac cgttaaaccg tcaagccgtc
     2761 aagccgtcaa accgtaatac ctacctcccc agcctttctc tcaacttctt cccggcctct
     2821 tcaaacccgg gcttattaag gagtgcaaac atattcttct tgtaggcttc cacacccggc
     2881 tggtcaaacg gattgacatc aaggatgtat ccactgatag cacaggccat ctcgaagaaa
     2941 taaataagct ggccgataaa aaattcatta agctccggaa tgttgatctt tattacaggt
     3001 acattaccgt cattatgagc caggatagtg ccggtttcag cattgttgtt gacctccgac
     3061 agtcttttcc cggcaaggta gttcagctgg tcggaatcat ctttttcggg aggtattgta
     3121 actttcttac gtgtaccggc aactgacaga aatgtctcaa agagtattct ctgaccttcc
     3181 tggatatatt gcccgagtga gtgaagatct gtagtaaaat cgactgatgc cgggaaaatc
     3241 cccttacctt ccttgccttc gctttcccca aagagctgtt tccaccattc ggaaaaataa
     3301 tgcagtcgcg gagtgaatcc gacaagtgtc tcaacgggcc ggccggtctg gaggagaagg
     3361 tttctggcag cagcatacat aagggaagga tttgatactg catccttgtt ttttcttgtg
     3421 ataagcgcca tttcgtgcgc tcccctgaca agcatcctga tatcggctcc ggcaacagca
     3481 atgggcagca gtccgacagg tgtgagaacc gaatatcttc caccgatatc gtcgggaatt
     3541 ataaatgtat cgtacccgtt aagatcggca agcgatctca atgcgccctt tgaactgtcg
     3601 gtgatggcaa tgatcatgtc cgcagccctg gcttttccga cccttctttc aagatgatcc
     3661 ttaaggatcc tgaaagctat agcaggctcg gttgtggttc ctgattttga gataactatt
     3721 attgaataat tcctgccatc caggatctca ataagatcgg acatgtaatc ttcgcaaata
     3781 ttctgacctg caaaaattat ctcatgatgt tttgatctca gtaacgggga gaaagaatgt
     3841 gagagtcctt cgatcactgc tttagctccc aggtaagagc caccgatacc gatgacgacc
     3901 gtagtatccg aaaccgatct cagatgtttt gcagtctttt ctatcctgtc gaattgtttt
     3961 acaatatcat cggggagaga gagccatccg aggaaatcat taccggcacc tgttcctgca
     4021 ttaagggaat cgagatgcct gattgactgt tgtgcaagag ccactacctc ttcataagag
     4081 ataaaacctg ctgcattctt aaggtctata ctgatatttt tttccatatg tagtaatttt
     4141 ttgtcagtgc tttcctgcaa agataaaaaa aaccgccggg gagtttccct ggcggcaatt
     4201 ataatatgac tatatataat gataaggaag ttatctgcga tgtattcccc cggcgctgga
     4261 gga
//
LOCUS       DAOOYG010000181         4173 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_25189_length_4173_cov_5.155415, whole genome shotgun sequence.
ACCESSION   DAOOYG010000181 DAOOYG010000000
VERSION     DAOOYG010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25189_length_4173_cov_5.155415"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="PK288_11090"
     CDS             <1..147
                     /locus_tag="PK288_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPM19515.1"
                     /translation="DTFATHIRLPETKRGDIIAIRSAGAYGESMASRYNMRDLPGSVF
                     SDEI"
     gene            complement(236..1828)
                     /locus_tag="PK288_11095"
     CDS             complement(236..1828)
                     /locus_tag="PK288_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPM19516.1"
                     /translation="MSKKQLFLTKPLGQLLSESKESGGLKRTLTALNLTTLGIGAIIG
                     AGIFVLSGQAAAQYAGPAIVLSFVVSGLACGFAGLCYAEFASMIPIAGSAYTYSYATL
                     GEFFAWIIGWDLILEYLFAASTVSVGWSGYVVSFLKDFNINVPPQFSDATGTVLVNIP
                     ELGWKPLHAFAHTLAESGINADALPHVTCILNLPAMFIIALLSTLQVIGIKESANFNN
                     LMVIIKVSVIVLFIAVGFAFVKSVNWHPFIPANKVESTPISEYGSFWLWLKAYGSEFG
                     KFGISGILRGAGVIFFAYIGFDAVSTAAQEARNPQRDMPVGILGSLGISTLLYILVAV
                     VMTGIVSYKTLNVADPVAVAVDAMGKGMFWLRPIVKIAAIAGLSSVILVMLLGQPRIF
                     FTISKDGLLPPVFSKVHPRFKTPYMSTILTGIVAMIIAGILPIRILGELVSIGTLLAF
                     IIVCISVLVLRKTRPEVERPFRTPWVPVVPILGALICFVQMAALPLDTWLRLLVWMAL
                     GFMIYFFYGIRHSKVRMANKKD"
     gene            1976..2689
                     /locus_tag="PK288_11100"
     CDS             1976..2689
                     /locus_tag="PK288_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPM19517.1"
                     /translation="MISKALMTMIRKFLLATLIILSACSPLKTYRNLPEVKAWENDIR
                     KFEQLDVEKSYPSDAVLFAGSSSIKLWDDIGKDMQPYNVIQRGYGGAKLSDFAVYADR
                     IIYPHPCSAIVIFIANDISGSDSDKTPAEVAELFRKTLYIIRRKYKDTPVFWIAITPT
                     AARWHVWPEIREANQQIQKICNNHHNTYFISTEQAFLTREGLPRTELFRDDKLHLNTD
                     GYRVWAGIIKNELYKVLGR"
     gene            2984..4057
                     /locus_tag="PK288_11105"
     CDS             2984..4057
                     /locus_tag="PK288_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HPM19518.1"
                     /translation="MRNISRRHFFFRTLGAGAGSLMFPVLTSGRSSSASKGITPLIVT
                     SHANETGRKAMEAGWQVLIEGGNAVDAVEKAANIIELDPEDTTVGYGGLPNEKGVVQL
                     DASFMDGRTYSAGAVACLENIKTPSSVAKLVMQRTDHVLLTGAGALEFAKAWGFREEN
                     LLTEKARKIWLRWKEELSPDDDWGPPEHLKDSDRKEAYWNDFPAPEHHYGTTNVLAID
                     SRGDIAGCTTTSGLSFKLNGRVGDSPIIGAGLYVDNETGAAGATGRGEDVIKSCASYY
                     IVLRMKEGRTPQQACEDALKMIAGKYKKVNPLFYPAEKFVAINRTGETGCAAMKGDRM
                     PEMSVRTAEGFSIYTGPVVFPGK"
BASE COUNT         1191 a         1031 c          989 g          962 t
ORIGIN      
        1 gatacttttg ccacacatat caggctgccc gagacaaaaa gaggtgatat cattgctata
       61 aggtctgccg gggcatatgg tgaatccatg gcttcccgct acaatatgcg tgatctgccc
      121 gggagtgtct tctccgacga gatctgaaaa cggctgcatt cctggaatcc ccggctatgt
      181 ttttcgatcc gcgcgaagtg tgcagatctt gcccgtaacg ccgacagaat gacggtcagt
      241 cctttttgtt agccatcctc accttgctgt gcctgatccc gtagaagaaa tatatcatga
      301 agccgagggc catccacaca agcagcctca gccatgtatc aagcggcagg gccgccatct
      361 ggacaaagca gataagggca cccagtatcg gtaccacggg tacccacgga gtcctgaagg
      421 gcctctccac ctcaggtctt gttttcctga gaaccaatac gcttatgcac acaatgatga
      481 acgccagcaa ggtcccgatg gatacaagct cccccaggat ccttatcggc aggatcccgg
      541 caatgatcat tgcaacaatc ccggtgagta tggtactcat atacggggtc ttaaaccttg
      601 gatgaacctt tgaaaaaaca ggaggaagca agccgtcctt tgatattgtg aaaaagatcc
      661 tcggctgtcc aaggagcata acgaggatta ctgagctcag ccctgcaatt gcagcaatct
      721 tgacaattgg cctgagccag aacataccct ttcccatggc atcgacggca actgccaccg
      781 gatcagcaac attaagggtt ttatagctca ctatccctgt catgacgaca gcaacaagga
      841 tataaagcaa cgttgatata ccgagtgatc caagaatgcc cacaggcatg tctctctgcg
      901 gattacgggc ttcctgggct gcagttgaaa cggcatcgaa cccgatatac gcaaagaata
      961 ttacgccggc ccccctcagg atcccgctta tcccgaattt accgaattct gatccgtaag
     1021 ctttcagcca taaccagaat gatccatatt ccgatattgg tgtggattca accttgtttg
     1081 ccggaatgaa cggatgccag ttgactgact ttacaaacgc aaaacccacg gcaataaaca
     1141 atacaatcac tgacaccttg ataataacca ttaagttgtt gaagttggct gattccttta
     1201 ttccaatcac ctgtaatgtt gatagcaatg ctataatgaa cattgccgga aggttaagaa
     1261 tacaggtgac atgcggaagc gcatcggcat ttatccccga ttcggccagt gtgtgtgcaa
     1321 aagcatgcag cggcttccat cccagttcgg gaatgttgac caggaccgta cctgtggcat
     1381 cggagaactg cggcggaaca tttatgttga aatccttgag aaagctgacc acatatcctg
     1441 accatccaac cgatactgtt gatgctgcaa aaagatattc caggataaga tcccagccta
     1501 ttatccatgc aaaaaactca cccagggttg cataggaata ggtataggcg ctcccggcaa
     1561 ttggtatcat cgaggcaaat tccgcatagc acaggccagc aaaaccgcag gccaggccgg
     1621 aaaccacaaa tgacagcact atggcgggcc cagcatactg tgcagcagcc tgaccgctga
     1681 gcacaaaaat gcctgcacca attatggcgc caataccaag ggttgtaaga ttcagcgccg
     1741 taagagtacg cttcaatccc cccgactcct ttgattcact cagtaactgc cccaggggct
     1801 tggtaagaaa taattgcttt ttcgacattg gaattaattg acaggattag catggaaaag
     1861 ttaaaatatt gcaaacgtaa cttttttttg taaatgaaat attgttacta ttgtattaaa
     1921 tcaaatcaga aattgatttt ttttttaaac ctttacatga attaacctaa ttttaatgat
     1981 atcaaaagca cttatgacca tgatacgtaa attcctgctt gcaaccctga tcatcctttc
     2041 agcctgttca ccgttgaaaa cctacaggaa tctgccggag gtgaaggcat gggaaaatga
     2101 tatccggaaa tttgaacagc tcgacgtaga aaaatcatat ccctccgacg cggttttgtt
     2161 tgcaggcagc tcaagcatca agctatggga tgacatcggc aaggacatgc aaccttacaa
     2221 tgtgatccag aggggctatg gtggcgccaa gctcagcgat ttcgcagtat atgccgacag
     2281 gatcatctat ccgcatccct gcagtgccat agtgatattc attgcaaacg acatctcggg
     2341 cagtgattct gacaaaaccc ctgcagaagt ggccgaactg ttcaggaaaa cattatacat
     2401 tatacgaagg aaatacaagg atactcccgt cttctggatc gcgatcacac ccacagccgc
     2461 cagatggcac gtctggcctg aaattaggga ggccaatcag cagatccaga aaatatgcaa
     2521 taatcatcat aatacatatt tcatcagcac cgaacaggca tttcttacca gggaaggcct
     2581 tccccgcacc gaactgttca gggacgacaa gcttcacctg aacacagacg gatacagggt
     2641 atgggcggga atcatcaaga atgagctgta caaggttctc ggaagataaa ggaacccggg
     2701 gtccgacata tgaaccggtt tccgtccggt ctgatctccg gcaggaaaac cccttgcctg
     2761 atgcatgata tgcaaatatg ccgcccgggc aggccggaca gcaggataaa tatggcattt
     2821 agctacaact gttcaaatta tcaccggtaa tggtttcaat actcacagat gagtgtacag
     2881 gaccccgcat gcctgatgct atgaatattt ttatgatcag ccgggttttt atccggctga
     2941 tctgattata tttgaactta actatcacaa acatcagcca gtcatgagaa acatatcccg
     3001 ccgtcatttt ttcttcagaa cccttggggc cggtgccggt tctcttatgt tcccggtact
     3061 tacttccggc aggtcttcat ctgcttcaaa gggaattaca cccctgatcg tcacaagcca
     3121 tgcaaacgaa accggccgga aggcaatgga agcaggctgg caggttctca ttgaaggagg
     3181 gaatgccgtc gatgcggttg aaaaagctgc aaacattata gaactggatc cggaagatac
     3241 aaccgtcggg tatggaggat tgcctaatga aaaaggcgtt gttcagcttg atgcatcctt
     3301 catggacggc agaacttact cagccggggc ggtggcctgc ctggaaaaca taaagactcc
     3361 ctcatcggtt gcaaagctgg ttatgcagcg caccgatcat gttttgctta caggcgcagg
     3421 tgcgcttgaa tttgcaaaag catggggatt cagggaagaa aaccttctga ccgaaaaggc
     3481 ccgtaaaatt tggctgagat ggaaggagga gctcagccct gacgacgact gggggccgcc
     3541 cgaacacctt aaggattcgg accggaagga ggcatactgg aacgatttcc ccgcaccgga
     3601 acatcactac ggcaccacca acgtgcttgc aattgactct aggggtgaca tagccggatg
     3661 caccactacc agcggactga gcttcaagct gaacggaagg gttggtgatt ctccgataat
     3721 cggggccgga ctgtatgtcg ataatgaaac tggcgccgcc ggcgcaacag gccgcggaga
     3781 agatgtgatc aaatcgtgcg cttcgtatta tattgtcctc aggatgaagg aaggaagaac
     3841 accccagcag gcatgcgaag atgcgctgaa aatgatcgcc ggcaagtata aaaaagtcaa
     3901 cccgctgttc tatcctgccg aaaaattcgt tgcaataaac aggacgggcg aaaccggatg
     3961 tgcagcaatg aagggcgacc ggatgccgga aatgtcggta aggactgctg aaggattttc
     4021 catttatacc ggacctgtcg tattcccggg caaatgaacg gctggttaag cagcgatccc
     4081 atcaataatg tatttcagtg acttccacca tacatcattt aataatcagc tgccgccatc
     4141 caataaagca taacccttat gttatgaaag ata
//
LOCUS       DAOOYG010000182         4127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_25559_length_4127_cov_5.687868, whole genome shotgun sequence.
ACCESSION   DAOOYG010000182 DAOOYG010000000
VERSION     DAOOYG010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25559_length_4127_cov_5.687868"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PK288_11110"
     CDS             complement(<1..971)
                     /locus_tag="PK288_11110"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPM19519.1"
                     /translation="MRINRKHFIIRSAAGAACLSAGSILPGFITGVSNSDRDIRSHNN
                     VNQGSFGCTGSGHIKKTPELLPDKIAGMTLEELRDDYRNRIFNQYLPFWEKGGYDSDL
                     GGFMCELDDDGRVVIDEKYIWYQARGIWVYSFLYNNFGREGKFLDIAGKSRDFMVKNM
                     YLGNGVWRESVDRQGNPVKSMVSQGSSNDIYGALFSAAGLIELYKANGNRDDLTIALD
                     SVRASAEAYESPDYEGIVVPGINRKGLRTIGHSFMFVWILGNLLSFLNDRNLKELQDE
                     HINHILNDFWNPEYGISNENLYHDYSRLPGYESTMYSGHYLETLWMVL"
     gene            complement(1029..3857)
                     /locus_tag="PK288_11115"
     CDS             complement(1029..3857)
                     /locus_tag="PK288_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HPM19520.1"
                     /translation="MLAGKYSELHEKLTRQIDKSRIYHDPLYTRAYGTDASFYRLIPK
                     LVIKAFDEDEVSFILRQCSDMNIPVTFRAAGTSLSGQAITDSVLLVAAGNWKKYRINE
                     DGSRIILQPGLTGAVINSLLAPYGRKIGPDPASINAATIGGIIANNASGMSSGTGANA
                     YNTIESLRLVFADGTVLDTGNEESRKAFIKSHQSLINDILNLSGSVRDNVQLAERIRH
                     KYILKNTTGYSLNALTDFTDPFQIIKHLIIGSEGTLAFISEVTMNTVVEHRFRASSLM
                     IFPDLEKACTAVALLGQGPVTAAELIDRAGLAAVADKKGMPGFLKDLSPGASALLVET
                     SSDSRDELTSQIDILTKAIGSIPVEIPVSFTGDPAKYAVYWDIRKGLFPSVGARRREG
                     TTVIIEDVAFPVEKLAGATADLQQLLKKSGYDDAVIYGHALEGNLHFIFSQDFSREEE
                     INRYRTLMDEVADLVTGKYDGSLKAEHGTGRNVAPFVAKEWGQEAYSVMQRIKQIFDP
                     KQILNPGVILNSDPEVHLKNLKPMPPAHESIDKCIECGFCEPSCVSADLTLTPRQRIS
                     VFREITRLAGTGHEPHLASSLVKAYAYAGDYTCATDGLCAITCPVNIDTGKLIKHLRL
                     EKIGNRQKLAGWLARNMNIVTAAARTGLNTVSFFHRILGTQVMKSIAGSMRKLSGKRI
                     PMWNPYMPGGAKKINVPATQHEPGKRKVVYFPSCINRSMGTSADHHGEIQLTEKMKSL
                     LEKGGYEIIYPDNLDNLCCGMAFLSKGYRKAGEMKSAELESALMQASNNGEYPVLCDM
                     SPCLFTMKDNFRTDLKLYEPVEFITKFLLHRLELTPLQETVTVFPVCSMKKMGLDDML
                     TDLARRCAADVITPDTNCCGFAGDRGFTFPELNRHGLRNLRSQIPGNVRNGYSTSRTC
                     EIGLSLHSGISHKSIVYLVDEVSRPKNN"
BASE COUNT          975 a         1028 c          967 g         1157 t
ORIGIN      
        1 agaaccatcc agagagtctc cagataatga ccggaataca ttgttgattc atatcccggc
       61 agccgggaat agtcgtgata cagattctcg ttgcttatgc cgtattcagg attccagaaa
      121 tcgtttagta tatgattaat atgttcatcc tgaagctctt tcagattccg gtcattaaga
      181 aatgaaagca aattgcccag gatccagaca aacatgaaag aatgaccgat agttctaagt
      241 cctttccggt taattcccgg aacgacgata ccttcatagt cagggctttc ataggcttcg
      301 gcggatgccc tgaccgaatc cagggcaatg gtgagatcgt cccggtttcc gtttgcctta
      361 taaagttcga tcaggcctgc agctgaaaaa agagctccgt aaatatcatt gctgctgccc
      421 tgggatacca tgcttttgac cggattgccc tgcctgtcaa ctgattcacg ccagacccca
      481 ttaccgagat acatgttttt taccatgaaa tccctcgatt ttccggcaat atcaagaaat
      541 ttgccttccc ttccgaaatt attatacaga aaggagtaga cccatatccc ccgggcctgg
      601 taccagatat acttttcatc gatgacgacc ctgccgtcgt catcgagttc gcacatgaag
      661 ccgcccaaat cactgtcgta tcctcccttc tcccagaaag gaagatactg gttaaaaatg
      721 cggttacggt agtcatccct gagttcttcc agcgtcattc ctgcgatttt atcagggagc
      781 agttccggtg tcttctttat atggcctgaa ccggtgcatc cgaatgaacc ctgatttaca
      841 ttgttatgtg acctgatatc cctgtcggag tttgagacgc cggtaataaa gccgggcagg
      901 atacttccgg ccgacagaca ggcagctccc gcagcactcc ttataataaa atgtttacgg
      961 ttgattctca ttttttcctg attcagttag gatccgggat ggatcagact atgatattca
     1021 gaatatgatt aattgttctt tggcctgctg acctcatcga caagataaac tattgattta
     1081 tgcgaaatgc ctgaatgaag gctcaggcct atctcgcagg tccggcttgt ggagtagccg
     1141 ttcctgacat taccggggat ctgtgaccgc aggttcctca atccgtgcct gttcagttcg
     1201 gggaaggtaa atccccggtc accggcaaat ccgcagcagt tggtgtcggg cgtaattaca
     1261 tcggcagcgc acctgcgtgc caggtcagtc agcatgtcgt caagtcccat ctttttcatg
     1321 ctacatacag ggaacactgt caccgtttcc tgcaggggag taagttcaag ccggtggaga
     1381 agaaatttcg ttatgaattc aaccggttca tacaacttca ggtccgttct gaaattatcc
     1441 ttcatcgtaa aaaggcacgg gctcatatcg caaagcaccg ggtattcacc gttgttgctt
     1501 gcttgcatga gggccgattc aagctcggcc gatttcattt ctcccgcctt cctgtatccc
     1561 ttgcttaaaa aagccatgcc gcagcacaga ttgtcgagat tgtccgggta gatgatctca
     1621 tagccgccct tttccagcag gcttttcatt ttttcggtaa gctgtatttc cccgtgatga
     1681 tccgctgatg ttcccatcga acggtttatg catgagggga aatagaccac ttttcttttt
     1741 cccggttcat gttgcgtggc tgggacattg atcttttttg ctccgccggg catgtatgga
     1801 ttccacattg gtatccgttt tcccgataac tttctcatgc ttccggcaat tgatttcatt
     1861 acctgtgttc ccaggattcg gtggaaaaaa ctgactgtat tcagtcctgt ccgggccgca
     1921 gctgtgacaa tattcatgtt ccgggccaac cagcctgcaa gtttctgcct gttgccgatc
     1981 ttttcgaggc gaaggtgctt tatcagcttc cccgtatcga tattaaccgg gcaggttatt
     2041 gcacacaatc cgtcggttgc gcatgtatag tcaccggcgt atgcatatgc ctttaccagt
     2101 gatgatgcaa gatgaggttc atggccggtg cctgccagtc ttgtaatttc cctgaaaacg
     2161 cttattctct gcctgggtgt aagggtcagg tcggcagaga cgcaggaggg ttcacagaat
     2221 ccgcattcaa tgcatttatc tattgattca tgggccggtg gcatcggttt caggttttta
     2281 agatggactt ccggatcgga attcaggatc actccgggat tgaggatctg tttcggatca
     2341 aaaatctgct tgatcctttg cattacagag taggcctcct gtccccattc cttagcgacg
     2401 aatggtgcca cattgcggcc ggtcccgtgt tctgccttca gggatccgtc atatttgccg
     2461 gtcacgagat ccgcaacctc gtccatcagt gtcctgtacc tgttgatttc ttcctccctg
     2521 ctgaaatcct ggctgaaaat aaaatgcaga ttgccttcca gagcatgtcc gtaaatcacc
     2581 gcatcatcat atcccgattt tttgagaagc tgttgcaggt cggccgtggc gcctgccagt
     2641 ttttcgacag ggaaagcaac atcctcgatg atcaccgtgg tgccttctcg ccgtcttgcc
     2701 ccgaccgagg gaaacagccc tttcctgata tcccagtata cagcatattt tgccggatca
     2761 ccggtaaaac tgaccggaat ttcaacaggt atgctgccga tggctttcgt taagatatca
     2821 atctgactgg tcagttcatc ccggctatcc gaagaggtct caaccagcag cgcgctggcg
     2881 ccgggcgaca gatccttcag gaaacccggc attccttttt tatcggctac ggcagcaagt
     2941 cccgccctgt caatgagttc agccgctgtt acaggtccct gccccagtag agccactgca
     3001 gtgcatgcct tttccagatc ggggaatatc atcagcgaac ttgcacggaa tcgatgttcc
     3061 actacggtat tcatggttac ttccgaaata aaggcaagag taccttccga tccgatgatg
     3121 agatgtttga taatctggaa gggatccgta aaatcagtca gtgcgttcag actgtagcct
     3181 gtagtgttct tgagaatgta tttgtgcctt atcctttcgg caagctgcac attatccctg
     3241 acagaccctg agaggttaag aatgtcgttt ataagcgact gatgggattt aatgaaagct
     3301 ttcctgcttt cttcattccc ggtgtcaaga acggttccgt cggcgaaaac aaggcggagg
     3361 ctctcaatgg tattataggc atttgcaccg gtcccgctgc tcatcccgct ggcattgttt
     3421 gcaatgatcc ctccaatcgt tgcagcattg atcgaagccg ggtcgggacc tatctttctg
     3481 ccatacgggg caagaagcga attaattaca gctcctgtca gtccgggctg caatatgatc
     3541 cttgagccgt cttcattaat ccggtatttt ttccagttcc cggctgcaac caaaagtacc
     3601 gaatcagtta tggcctgtcc tgaaagactg gttccggcag ccctgaaggt tacgggaata
     3661 ttcatatctg aacactgcct gaggatgaat gaaacctcat cctcatcaaa agctttgatc
     3721 accagcttcg ggatcaggcg gtaaaagctt gcatcggtcc cgtacgctcg tgtataaaga
     3781 ggatcgtgat aaatccggga tttatcgatc tgccttgtca gtttttcatg gagttcggaa
     3841 tatttcccag caagcatatg gtttaatgtt tttgatggat cgtaacaaaa atacctgcaa
     3901 ctgatatcct gtaaatatgc ccggctctac tgttaattga ttaacagtgc ataccgcgga
     3961 gaacacggcg gcaacgggat atgataaaat gatcacctga tcagggttgc ctgtgtgttc
     4021 ccctggatta attgtttaag tcgcaatgaa gcaagaacac gacggctgat ttgtaatcaa
     4081 agttacaaac agtgtccata tttctgcgtt aaggatatcg aacagga
//
LOCUS       DAOOYG010000183         3996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_26734_length_3996_cov_6.845724, whole genome shotgun sequence.
ACCESSION   DAOOYG010000183 DAOOYG010000000
VERSION     DAOOYG010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26734_length_3996_cov_6.845724"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..1267
                     /gene="nqrF"
                     /locus_tag="PK288_11120"
     CDS             14..1267
                     /gene="nqrF"
                     /locus_tag="PK288_11120"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HPM19521.1"
                     /translation="MILAVTTLSTLLFSVVVFLIIILLLVMMLLFARDRLLPKGNVKL
                     QVNDRELEVSQGGNLLSTLSGNGIYLPSACGGAGTCGMCRCQVVSGGGSILPTEVVFF
                     TRKEQQNNWRLGCQVKIRDNMSIRLPESVFGIRKWECEVVSNKNVATFIKELVVKLPD
                     GEDLDFEPGGYIQIDVPKYETSFRDFNIDEIFRSDWDKYNMWDLHVKNTSETYRAYSM
                     ANYPAEKNLIKLNIRIATPPWDNLEKRFMNVPPGICSSYIFSLKKGDKIVISGPYGEF
                     HIKDTPNEMVYIGGGAGMAPLRSHIFHLFHTLRTGRKVSFWYGARSLREVFYEEEFIA
                     IEKKFNNFRFHLALSEPLPEDNWKGPTGFIHQVLLDNYLSKHEEPEDIEYYFCGPPVM
                     NDAVVKMLDSLGVPQENISFDDFGI"
     gene            1380..2399
                     /locus_tag="PK288_11125"
     CDS             1380..2399
                     /locus_tag="PK288_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPM19522.1"
                     /translation="MVLKKSFFFLGIAILLQSCTNTKVYDTFTGFVQGTTYRMIFENS
                     GKINPVELKSEVEKLLFDYDMSLSVYRDSSIVSKINRNEDVVPDAWFASVFARSEEIN
                     RLTGGDFDITVGPLVNAMGFGPDAHKHFNAQMRDSLMELVGMEKVKMVNGKIEKSDPR
                     IKLDFNAIAKGYSVDVVSGFFDGKGIKSYLVEIGGEIRVKGSKNGKLWKIGIDRPEDS
                     NFVPGNHLQAVLRLKDRSLATSGNYRKFYVEDGIKYSHTIDPKTGMPARNQLLSATIL
                     ASDCATADGIATACMVKGKDETIEFLEKHPEFDAFLIFSDDSGNFSTWMTSGLRNYIS
                     EPENK"
     gene            complement(2415..2669)
                     /locus_tag="PK288_11130"
     CDS             complement(2415..2669)
                     /locus_tag="PK288_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19523.1"
                     /translation="MFVKLLILSAVLLSLAALFFGVRILLKNNGVFPEIHISQNREMR
                     KRGISCGGDPDIGCSSPGGFKECPSCGKILPRMEPEPENV"
     gene            2829..>3996
                     /locus_tag="PK288_11135"
     CDS             2829..>3996
                     /locus_tag="PK288_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HPM19524.1"
                     /translation="MDKSNNTTGQLPDRNTILADFRLATLSRTLSIIGRREVLSGKAK
                     FGIFGDGKEIVQIALAKQFRDGDWRSGYYRDQTWMMAMGLYNAVDFFHQLYGNTDRKI
                     IHGSGGRVFNNHFSVPNINPDGSWKNLMQQKNSSSDISPTAGQMPRLLGLAYASKLFR
                     QNSELHGYTTLSMQGNEVAFGSIGDASTSEGHFWESINAAAVLQVPMALSVYDDGYGI
                     SVPKKYQTAKGSISDILRGFEDEPGKPGILIFKGKGWDYRGLIDLYEKGIAICRRDHK
                     PVVFHIEEVTQPLGHSTSGSHERYKSAERLKWEVDFDPIAKMREWIVSEGIATDSELD
                     ALAADAEGEAREARKKAWNMFQQPIRVERDALVKIIEDRTCRCNDEIKEASVDEI"
BASE COUNT         1148 a          789 c         1007 g         1052 t
ORIGIN      
        1 taagtatttg attatgattc ttgcagttac cacattaagt actctactgt tctcagttgt
       61 tgtctttctt attattatcc tgcttctcgt aatgatgctg ctttttgcaa gggacaggct
      121 gctgcctaag ggtaatgtaa aattacaggt aaatgaccgg gagctggagg tatcacaggg
      181 aggaaatctt ctgtcaaccc tttcaggaaa cgggatatat ctgccctcag catgcggcgg
      241 tgccgggaca tgtggtatgt gccggtgcca ggttgtttcg ggtggaggat caatacttcc
      301 gactgaggta gtgtttttta cgagaaaaga gcagcaaaac aactggcgtc ttggctgcca
      361 ggtaaaaata agggacaata tgtcgatccg gcttcccgaa tctgtgttcg gaatcagaaa
      421 atgggaatgt gaagttgttt caaataagaa tgtggcaacc ttcatcaagg aactggttgt
      481 aaaactgccc gatggcgagg atcttgattt tgaaccggga ggttatattc agattgatgt
      541 tcccaaatac gaaaccagtt tcagggattt caatattgac gagatcttca gaagcgactg
      601 ggataaatac aatatgtggg accttcatgt aaagaacacg agtgagacat acagggccta
      661 ttcgatggcg aactatccgg cggaaaagaa tctgattaaa ctcaatatca ggattgcaac
      721 acctccatgg gacaatttgg agaagagatt catgaatgtc ccccccggga tctgctcatc
      781 atacatcttt tcgctgaaaa agggtgataa aatcgttata tccggcccct atggtgagtt
      841 ccatatcaag gacacgccca atgaaatggt ttatatcggg ggcggtgccg ggatggcgcc
      901 tctcagatcg catattttcc atctttttca tacgctcaga acgggcagga aagtttcatt
      961 ctggtacgga gcccggtcgc tcagggaagt cttttatgaa gaggagttta tcgcaattga
     1021 aaagaaattc aataatttca gatttcatct cgctctttct gagcctcttc cggaagataa
     1081 ctggaagggt ccgacaggat tcattcatca ggttctcctg gataattatc tcagcaagca
     1141 tgaggaaccg gaggatattg aatactattt ctgcgggcct cccgtaatga acgatgctgt
     1201 tgtaaaaatg ctcgattcgc tgggcgttcc gcaggaaaac atatcttttg atgattttgg
     1261 gatataaata cccgttcagg catcagttac tccccggatt cattcaaatg taaatattca
     1321 tccggcagaa accgggttaa gttgaattaa aagcaaaagc tgcattactc ttattgaaaa
     1381 tggttctgaa aaaatccttc tttttcctgg gcattgcaat tctgctgcaa tcgtgcacca
     1441 acactaaggt atatgataca tttaccggtt ttgtccaggg tacaacctac cgcatgattt
     1501 tcgaaaacag cggaaaaata aaccccgtgg aacttaaatc agaagttgag aaactccttt
     1561 ttgactatga tatgtccctg tcggtttacc gcgattcatc gatagtttca aaaatcaaca
     1621 ggaatgagga tgttgttcca gatgcctggt ttgccagtgt ttttgccaga tcggaagaga
     1681 taaacaggct tacaggaggt gattttgata taaccgtagg gcctcttgta aatgcaatgg
     1741 ggttcgggcc ggatgctcat aaacatttca atgcgcagat gcgcgacagc ctgatggaac
     1801 tggttggaat ggagaaggta aagatggtaa acgggaagat tgaaaagagc gatcctcgta
     1861 taaaactgga tttcaatgca atagctaagg ggtattccgt tgacgttgta tcaggatttt
     1921 ttgacggcaa gggtataaaa agctaccttg tcgaaatagg gggtgagata agagtgaaag
     1981 gaagcaagaa cggcaaattg tggaagatag gaatagacag gcctgaagac agtaattttg
     2041 ttccggggaa tcaccttcag gcagtcctca gactgaagga ccgttcactg gcaacatcgg
     2101 gtaattacag gaaattttat gtggaggacg gaataaaata ttcacacacc attgatccca
     2161 agacaggtat gccggcaagg aaccagcttc tgagcgctac aatcctggct tctgactgcg
     2221 ccactgccga cgggatcgca acagcatgca tggtaaaggg aaaagatgaa acaattgaat
     2281 ttctggaaaa gcatcctgag ttcgatgcgt tcctgatctt ttccgatgac agcgggaatt
     2341 tcagcacctg gatgaccagt ggtctcagga attatatttc agaacccgaa aataaatgat
     2401 ttccgggagg gcagttaaac gttttccggt tcaggctcca tccggggcag tattttaccg
     2461 catgacgggc attccttaaa accacccggg gatgaacaac cgatatcagg atctcctccg
     2521 cagctaatac ctcttttacg catttccctg ttttggctga tatgtatttc aggaaagacc
     2581 ccgttatttt tcagcaatat cctgaccccg aagaacagcg ctgccagcga aagcaataca
     2641 gcactaagta tgagcagttt tacaaacatt ttttgaactt cccttttctt ttgtaacttt
     2701 atggaatgac taaacctttt tgtttgttag tgttctgcaa aggtatttat cttttccgga
     2761 tacatattaa acaaccccgg gtttagtttg ttttatcact gacagaaata aaagtaacag
     2821 aatcagaaat ggataagagt aataatacaa ccggccagtt gcccgacagg aatacaatcc
     2881 tggctgattt caggcttgcc accctgagca ggacgcttag catcataggt aggcgggagg
     2941 tattgtcggg taaggcaaaa ttcgggattt tcggcgatgg aaaggagatt gtccagattg
     3001 ccctagcaaa gcaattcaga gacggcgact ggcgttcagg ttattacagg gatcagacct
     3061 ggatgatggc catggggctc tacaatgccg ttgacttttt tcatcagctt tatggaaaca
     3121 cagacaggaa aatcattcat gggagcgggg gcagggtgtt taataatcat ttttcagttc
     3181 cgaatattaa tcccgatggt tcgtggaaga acctgatgca gcagaaaaac tcctcttcgg
     3241 atatatcgcc tactgcaggc cagatgcctc ggttgctggg gttggcatat gcttcgaaac
     3301 tcttcaggca gaacagtgaa ctgcatggtt atacgacact ttcaatgcag ggaaacgagg
     3361 ttgcattcgg atcaatcgga gacgcaagca catccgaagg gcatttctgg gagtcaatca
     3421 atgcggcagc ggtattacag gtaccgatgg cgctttcagt gtacgacgac gggtatggaa
     3481 tatcggttcc caaaaagtac cagacagcaa agggaagtat ttcagatatt ctcaggggtt
     3541 ttgaggatga acccggaaaa ccggggatac tcatttttaa aggcaaggga tgggattatc
     3601 ggggactgat tgacctttac gaaaagggaa ttgcaatttg ccgccgcgat cacaaaccgg
     3661 ttgtatttca tattgaggaa gtgacccagc ctcttggtca ttcgacctcc gggtcgcatg
     3721 agagatacaa gagtgcggaa agattaaagt gggaagtgga ttttgatcca attgcaaaaa
     3781 tgcgtgagtg gattgtcagt gagggtattg caacggattc ggagctggat gcacttgccg
     3841 ctgatgcaga aggggaagcc agggaggcaa ggaaaaaagc gtggaacatg tttcagcaac
     3901 caatcagggt tgaaagggat gcacttgtaa aaattattga ggatcgcacc tgccggtgca
     3961 atgatgagat aaaggaggct tccgtcgacg aaatta
//
LOCUS       DAOOYG010000184         3991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_26785_length_3991_cov_6.648882, whole genome shotgun sequence.
ACCESSION   DAOOYG010000184 DAOOYG010000000
VERSION     DAOOYG010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26785_length_3991_cov_6.648882"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PK288_11140"
     CDS             complement(<1..831)
                     /locus_tag="PK288_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19525.1"
                     /translation="MKRNELKKLLPILTAIMLMVSSCEKTIDEPPLTINNLKGMFVPC
                     EGIFGQANSEISFYDSRTGQTTKSLFYSVNGVAPGDVVQAFEIADTLGFIVVNNSQKV
                     IVVNMKDFRVVKTIDGFSYPRSVVRADDKSVYVSNGNGTSDNYISRISLSTLEITGTL
                     TLNTGPERLIAVKSKVFAAISGGWNNDGNTVIEIDPVSFTVINTYTVASIPVDIVSDK
                     DNNIWAYCKGIPDYTNWPEVTYSGAGLSKINTSAATVTTFAIDNMASPGINNIAASRD
                     G"
     gene            complement(863..2779)
                     /locus_tag="PK288_11145"
     CDS             complement(863..2779)
                     /locus_tag="PK288_11145"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPM19526.1"
                     /translation="MRTRIVLHTRLTLLMIMSMTASCTVIFSQSAADTVIRIPDVEIK
                     ASRYQQFGNDIKTDEYHQDELSPFSGESLGRFLISKTALNVRSYGVGGALSNISLRGS
                     STSQVQVNWNGFPVNSVTLGSCDFSLIPVDGFDNISVVYGAPGALYGSGTFGGAVNLN
                     NDLKTADKFKGSVNAGYESLKTINGSVSITLGTGKLAWKINAWGALSGNEFNYYDYIG
                     QTERKQTDGQWTNAGAIQQFMFRLSSTSTIEAGLWYQVKAFNIPSRIGSVSYEFQRDS
                     TLKFHTGYKKSGNRWNLEAKAAVFTDHQRYAQKASAQSSSYSIDSRIKSNQIYGDINF
                     RYHPAPSLSVDAGLIGSFINASVTAYGQRKEEKGLTAFTGFKYDKNRLSVQAEFRKEW
                     NSNFSSGLLSSFGIARQMKPDKWRLRFNVSQKFRKPTFNDLFWMPGGNLGLKPETGYS
                     VETGSTVTIFDKGNARLSADAGIYWSEINNMIVWRPDGAYWSAMNYQRVHSAGVDGKL
                     ILDVKHRRLKYLSTLNLMLNKSDIKTYVSGEHEKMLYSPRIITVWENNLSAGIFDLVI
                     CHRFTADRFYDDNSLLNPYQILDIRTGVRIPAGKGKLGIHPGVYNLTNTTYELIRLFP
                     MPGRYWSVKINYSF"
     regulatory      complement(2926..3129)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3339..3680
                     /locus_tag="PK288_11150"
     CDS             3339..3680
                     /locus_tag="PK288_11150"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPM19527.1"
                     /translation="MAGDPSLITDETDRLESEQRNTDPLLVKYFVMSCMAVTRKIMKS
                     MVKIARQADYTLLFLCGENDKIVDKSGCDEIFSAWNCQRKSYVIIKGGSHGKSTVVKG
                     AGLIAGWLEGI"
BASE COUNT         1083 a          877 c          884 g         1147 t
ORIGIN      
        1 gccatccctg cttgccgcga tattattgat tcccggggag gccatgttgt caattgcgaa
       61 tgtggtgact gttgcagccg aagtgttgat cttgcttaac cctgcacccg aataggttac
      121 ttcgggccag tttgtataat ccggaatacc tttgcagtat gcccatatat tgttgtcttt
      181 atcggatact atatcaacgg gaattgaggc cacggtgtag gtgttgatga ctgtaaagga
      241 aacagggtca atctcgataa ccgtgttgcc gtcattgttc catccgccag aaattgccgc
      301 aaaaactttt gatttcacgg ctatcagcct ttcgggtccg gtattcagcg tcaaagttcc
      361 ggttatttca agggtcgaca ggctgatcct gctgatatag ttatcagagg tgccgttgcc
      421 attggagaca tatacggact tatcatcggc acggacaacg ctgcgtggat aggaaaaacc
      481 gtcaatggtt ttgacaactc tgaagtcctt catgtttaca acgataacct tttgtgagtt
      541 attaacaaca atgaagccga gtgtatccgc tatttcaaat gcctgaacca catcgccggg
      601 cgccactccg ttaacggaat agaaaagact ttttgtcgtc tggccggtcc gggaatcgta
      661 aaaacttatt tcactgtttg cctggccaaa tataccttca cacggcacga acattccttt
      721 caggttgttg attgtcaggg gtggttcatc aatggttttt tcacaggatg agaccatcag
      781 cataattgcc gttaaaatag gaagcagttt tttcaattca tttcttttca taaacgttgg
      841 atttaaaatg attcacatta aactagaagg aataatttat tttgactgac cagtaacgac
      901 cgggcatcgg aaacagacgg ataagctcat aagtggtatt tgtaaggttg tacacgcccg
      961 ggtggatccc cagttttcct tttcctgccg gaatcctgac accggtcctt atgtcgagaa
     1021 tctgataagg attcagtaag gaattatcat cgtaaaagcg atccgctgta aaacgatggc
     1081 agataaccag atcaaatatc ccggcagata agttgttctc ccatacggtg attatccgcg
     1141 gagaatacag cattttttca tgctctcccg aaacataggt ttttatgtcc gacttattca
     1201 gcatcagatt aagtgtcgaa agatatttta gccttctgtg ttttacgtca aggataagct
     1261 tgccgtccac ccctgcagaa tggacacgct ggtaattcat ggcggaccag tatgcaccgt
     1321 cgggccgcca gactatcata ttatttattt ctgaccagta aatacccgca tcggcagaga
     1381 gcctggcgtt tcctttatca aaaatcgtta ccgttgatcc ggtctcaacc gaataacctg
     1441 tttcaggttt aagaccaaga ttgccccccg gcatccagaa aaggtcattg aatgtcggtt
     1501 tgcgaaactt ctgcgaaaca ttgaacctca gcctccactt atcaggtttc atctgccttg
     1561 ctatcccgaa agaggacaat agacccgagc tgaaattgga gttccattct tttctgaact
     1621 cagcctggac tgaaagccgg tttttatcgt atttaaatcc ggtaaaagcg gtcagtcctt
     1681 tctcctcttt tctctgcccg taagccgtca ccgatgcatt gatgaatgaa ccgatcagcc
     1741 ctgcgtcgac cgacaggctg ggagcaggat ggtacctgaa attaatatcg ccatagatct
     1801 gatttgattt gatccttgag tcgatgctgt atgatgaaga ctgcgccgat gctttctgag
     1861 catatctctg gtgatcggtg aacactgcgg ccttagcctc aaggttccag cggttccctg
     1921 attttttata gccggtatgg aatttcaggg ttgaatccct ttgaaactcg taagacaccg
     1981 atcctatgcg ggatggaatg ttaaatgcct ttacctggta ccataatccc gcttcaatgg
     2041 ttgatgttga tgacaaccgg aacataaact gctgaatggc gcctgcattg gtccattgtc
     2101 cgtctgtctg ttttctttcg gtctgtccga tataatcgta atagttaaac tcattgccgg
     2161 aaagagcgcc ccacgcgtta attttccagg ccagcttgcc ggttcctaaa gtgattgata
     2221 cgctgccgtt tatcgttttg agtgattcat aacctgcatt tactgatccc ttaaacttat
     2281 ccgctgtttt caggtcatta ttcaggttga cagctccgcc aaatgttccg cttccgtaga
     2341 gagctcccgg ggccccgtaa acaacagata tattatcgaa tccgtcaacc ggtataagag
     2401 aaaaatcaca tgaacccagg gtgaccgaat tgacaggaaa accgttccag ttaacctgga
     2461 cctggcttgt tgatgaaccc cgcagggaga tattgctcag tgcaccgcca actccatagc
     2521 tcctgacatt cagggcagtt ttgctgatca ggaagcgtcc cagtgattca cccgaaaaag
     2581 gactaagctc atcctggtga tactcatctg tttttatatc gttcccgaat tgctgatacc
     2641 gggaggcttt gatttccaca tcagggatcc ggatcacggt atcagcggcg gactgcgaaa
     2701 agatcacggt gcaggatgct gtcatagaca ttatcatcaa cagcgtcagt ctggtatgta
     2761 aaacgatcct tgtcctcatt cctgtcaata attcattgcg taaacattgc gacagctgca
     2821 tcggtaaatg aaattgcagt ttacatgcag gatccttcgc agaaataatc gaaaggcaga
     2881 gggatggaaa catgacaggt ttcattcttt gcctttcacc cgaaagctac gaagcatgtt
     2941 acaggcaggt cttctggctc atcctgtttt ggctgccttc ccatttcact ttaagctaaa
     3001 cagtggcagg gtatgccaaa acgcttgcag gacttacagc tgcgggtaca gctcctgaat
     3061 ttaacaggat tccctcttcg cccatgtcag atagttactg acatgagaac ctgtaatcgc
     3121 agcacaaata tattgatatt tcatatactg cttcttcccg aaaatgaaaa gtaattaaca
     3181 atctgccgta cacatggcag gttacagtgg gatggaccta gtggctaaag gaaaagaagg
     3241 agactgcagt ttttgtgttt tgtgtcataa aaaacagaca gtgatattca gcggattatg
     3301 aaagtcgtat ttgaagatat catttaccgg taataaatat ggcaggtgat ccttccctga
     3361 taacggatga aactgaccgg cttgaatcag aacaaagaaa cacggatcct cttttggtta
     3421 aatatttcgt tatgagctgt atggcggtaa caaggaagat catgaagtca atggtaaaaa
     3481 tagcccggca ggcagattat acgttgctgt tcctatgtgg ggaaaatgac aagatagttg
     3541 acaaatccgg ttgcgatgag atattttctg cctggaactg tcagcgaaaa agctatgtaa
     3601 tcataaaagg cgggtcgcac ggcaaatcga ccgtggttaa aggagccgga ctcattgccg
     3661 gatggctgga gggcatttaa gtggattgtc agatcaagta ttgactatta catctaagtc
     3721 caaaccgtct gttttgatga acaaatatca aaatatctca gatcttcttg ataaaagtga
     3781 tctatttgtc agacgcattt ccggaataac tggacattca attaatactg ttcggaaaac
     3841 caagcagtta aaagagattt gagtttagta gatttttcat aaaactatcg ctaaatgata
     3901 cttttgtgaa atatttacta ttgtgaaagc tcaatgttaa ctactcccca ttcttaagac
     3961 cactatttct cttttcttaa ttgattataa t
//
LOCUS       DAOOYG010000185         3934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_27338_length_3934_cov_8.074246, whole genome shotgun sequence.
ACCESSION   DAOOYG010000185 DAOOYG010000000
VERSION     DAOOYG010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27338_length_3934_cov_8.074246"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PK288_11155"
     CDS             complement(<1..1315)
                     /locus_tag="PK288_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19528.1"
                     /translation="MRILYFFSGFIISFSALYGQPPVGAWTDHLDYGSAVQLAVSPSK
                     IYASTGEALIIYNKEFSELTKLSTVNGLTETGISCIAWSDDSDLLIVAYKSANIDLLS
                     GNTIYNIPEIMFGTAGEISIQRIRTSGRYAYLATDYGIIVIDLIRREIRDTWKPGSDS
                     GGNRVYDVATGNGLIYAATENGVWYGELANTGLSYFGNWKQVTGLPELRSNLLIYSGG
                     RLYANYPMQFPEGDQVYSAGSDVYLFSSSAGIQNRSFDAAPGGFSIASGEMARVYNTA
                     GALINSIRSYGWGNPDICQAIIDAGDAWIADKTHGLVHGAGMTDFRKFTLPGPASDKV
                     VNITTLNGTTLICAGGTDGSWEELGRDFQVSIHAKGSFSNIISGNAKDAMRCCFDPSD
                     HSHFFVSSWGHGLFEYRNNLMINHYHSGNSPLGDGAGTDPAAMLCG"
     gene            complement(1330..1929)
                     /locus_tag="PK288_11160"
     CDS             complement(1330..1929)
                     /locus_tag="PK288_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HPM19529.1"
                     /translation="MDIVFATNNRHKLTEINNLIGKSFCLLSLADINLDNDIPENNPT
                     IEENALFKAWYVYRATGRNVFADDTGLEVDALGGEPGVHSARFAGENKNSDANIDKLL
                     ALLGDHKNRRARFRTIIALIFNGAEYLFEGIAEGTIIKSRRGRDGFGYDPVFIPDGYD
                     VTFAEMTLQEKNKISHRAIAFEKLRVFLNGIPEAGHDET"
     gene            2216..3625
                     /locus_tag="PK288_11165"
     CDS             2216..3625
                     /locus_tag="PK288_11165"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPM19530.1"
                     /translation="MVIIVLLLLILLVTGIYFFLRNQFAAKKQRLEQLLSIRAADLIA
                     EKDKTDALLANVLPKNTAEEIKSTGKAAKVKYNFVTVLFSDIQGFTRIAEEMNPEVLI
                     DELDKFFFHFDSVVDKYKIEKIKTIGDAYMCAGGIPEKDRANPVKVILAAMEMQNYMG
                     KLKAESVSGGSKYWDIRIGIHTGTVIAGVVGQKKLSYDIWGDTVNTASRMESSGEPGK
                     INISGTTYEFVKDFFDCKHRGRMPVKYKGEVDMYFVEGIVPELRNESGLPNDMFLFRM
                     QLIRLEDVEEAVIRLFTDDAPPNLYFHNLTMAKSIVAQVELICRSEQVPEKTFFVLKL
                     ASLFLITGYITDYENPEAASLSFMDEILPRYSLDAELVNESRLLIRNSYNEIYNTMAD
                     RILHDARYEYLGRNDYIRLSDRLLKERTEHGLEIGKEGWEETQKQLLERHEFLTETAR
                     LLRSVSIQEQTDALKAHNK"
     gene            3706..>3934
                     /locus_tag="PK288_11170"
     CDS             3706..>3934
                     /locus_tag="PK288_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HPM19531.1"
                     /translation="MENLDWKNLSFGYIKTDYNIRCFYKDGKWGELIVSDSEYIDIHI
                     AATALHYGQEAFEGLKAYMGKDGRIRLFRWEE"
BASE COUNT         1067 a          870 c          932 g         1065 t
ORIGIN      
        1 ttccgcatag catggccgca ggatctgttc ccgcaccatc gcccagtggc gaattaccac
       61 tgtgatagtg attgatcatc agattattcc tgtattcaaa cagtccgtgc ccccatgatg
      121 atacaaaaaa atggctgtgg tcggacggat cgaagcagca tctcattgca tcttttgcat
      181 ttcctgaaat aatgttggaa aagctgccct ttgcatggat ggaaacctgg aaatcccttc
      241 ccagttcttc ccatgaaccg tcagttccgc cagcgcagat gagtgtcgtg ccattaaggg
      301 tcgttatatt tacaaccttg tcggaggcag gtcccggcag ggtgaatttc ctgaagtcag
      361 tcatacctgc cccatgcaca agtccgtgcg ttttgtctgc tatccaggca tcgccggcat
      421 cgattattgc ctggcagata tcaggatttc cccagccgta cgaacggata ctgttaataa
      481 gggctcctgc cgtattgtaa actcttgcca tttcccctga ggcaatactg aagcctcccg
      541 gcgccgcgtc aaatgagcgg ttttgtatgc ctgctgaaga tgagaacaga tacacgtctg
      601 aaccagccga ataaacctga tcgccttccg ggaactgcat gggataattg gcatagagcc
      661 tgcctcctga atagatcagc aggtttgacc gtaattccgg taaaccggtt acctgtttcc
      721 agtttccgaa atacgacaat ccggtgtttg ctaattcacc ataccagaca ccgttttccg
      781 ttgctgcata aatcagtcca ttccctgtgg ctacatcata taccctgttg ccgcctgaat
      841 cggacccggg cttccatgta tcacggatct cccggcggat aaggtcgatg acaattatgc
      901 cgtaatcagt tgcaagatat gcgtagcggc cagatgtcct gatccgttgt attgaaattt
      961 ccccggcagt tccgaacata atctcgggga tattataaat cgtgttcccg gataaaagat
     1021 caatgttggc acttttatat gcaacgatga gcaaatcact atcatccgac catgcaatac
     1081 agcttatgcc tgtttccgtg agaccgttca cggttgacaa ttttgtaagc tcactgaact
     1141 ctttgttgta gataatcagc gcttcacctg ttgaggcata tatttttgac ggactgacag
     1201 caagctgtac tgcagaacca taatccaggt gatcggtcca tgctcctacc ggcggctgtc
     1261 catacagagc cgaaaaactg ataataaatc ccgaaaaaaa atacaaaatt ctcattatga
     1321 ccctgatcct taggtttcat cgtgaccggc ttccggaatg ccgtttaaaa ataccctgag
     1381 cttctcaaat gcaatggccc ggtgtgagat cttatttttt tcctgcaggg tcatctcggc
     1441 aaaagtaaca tcatatccgt cgggtataaa aaccggatca tagccaaacc cgtcccttcc
     1501 cctccggctc ttgattatcg tgccctccgc aatcccttca aaaagatatt cagcgccgtt
     1561 aaagatcagg gcaataatgg tccggaatct cgctctgcgg tttttatggt caccaaggag
     1621 agccagcagt ttatcgatat ttgcatcaga gtttttgttc tcacctgcga atctggctga
     1681 atggactccg ggttctccgc ccagtgcatc cacttccagg ccggtatcat ccgcaaaaac
     1741 attgcgccct gttgccctgt acacgtacca ggccttgaaa agcgcatttt cttcgatggt
     1801 cggattgttt tccgggatat cattatcaag gttgatgtca gccaggctga gaagacagaa
     1861 acttttaccg atcagattat tgatttcggt gagtttatgc ctgttgtttg tggcgaatac
     1921 aatgtccata ttgtgggttt atccgtttgc aatattacta aaaaaaagcc ccggccggag
     1981 gctgcattgt cccggtaatg aatacccggc agtgataaac actgtatgaa gattgaaagt
     2041 taattgatac atttggatta gaaacaggaa tcagatagtg caggaacaat ttattttgcg
     2101 gttgaggtat tttctgctga caattttgct cttttcaggg atgccttcat tgttgcgggc
     2161 cagagcattc acactgcagg atccgccccc ggaatcctgg tgcttttcca aactcatggt
     2221 tattattgtc cttctgctgt tgattcttct ggtcacgggg atctatttct ttctaaggaa
     2281 tcagtttgcc gccaaaaagc aaaggctgga acagctttta agcatcaggg cggccgacct
     2341 tattgccgaa aaagacaaaa cagatgccct ccttgcaaat gttctgccaa aaaatacggc
     2401 agaggaaatt aaatcaaccg gaaaagctgc aaaggtcaag tataatttcg ttactgtcct
     2461 gttttcggat atccagggat ttacccggat cgctgaggag atgaaccctg aggtgctgat
     2521 agatgagctt gacaaattct tttttcattt cgactccgtt gttgacaaat acaaaattga
     2581 aaagatcaag accattggcg atgcttatat gtgtgcagga gggattccgg aaaaagacag
     2641 ggcaaatccg gtaaaggtga tactggcagc gatggagatg caaaattaca tgggaaagct
     2701 taaggctgaa tcagtatcgg gaggaagcaa atactgggat atccggatcg gcatacacac
     2761 cgggacggtg atagcaggag ttgtgggtca gaaaaaactt tcgtacgata tctggggaga
     2821 tacagtgaac actgcaagcc ggatggaatc atcgggcgag cccgggaaaa ttaatatttc
     2881 aggaaccacg tatgaatttg taaaggactt ttttgattgt aaacaccggg gaagaatgcc
     2941 ggttaagtat aaaggcgaag tggatatgta ctttgttgag ggaatagttc cggaattgcg
     3001 aaatgaatcc ggactgccga atgatatgtt cctgttccgc atgcagctga tcaggctgga
     3061 ggatgttgaa gaagccgtga taaggctgtt tactgacgat gctcccccca atctctattt
     3121 tcataatctt acgatggcaa agagtattgt tgcacaggtt gagctgatct gccgctcaga
     3181 gcaggttcct gagaagactt ttttcgtgct taaactggct tcactctttc tgattacggg
     3241 ttatatcacc gattatgaaa atcccgaagc agccagcctg tcatttatgg atgagatcct
     3301 gccgcgttac agtctcgatg cagagctggt gaacgagtcg aggttactta tcaggaattc
     3361 atataatgag atttataata ccatggccga cagaatactt catgatgcaa gatatgaata
     3421 tctcggccgc aatgattaca ttcggctgag tgacaggctg ttgaaagaga gaaccgagca
     3481 tggccttgag atcggaaagg aaggatggga agaaacccag aaacagcttc ttgaaaggca
     3541 tgaattccta acagaaactg ccaggttatt gagaagtgtc agtatacagg agcagacaga
     3601 tgcccttaaa gcacacaata aatagaacaa tttgggcatt gattctgccg caatttgtca
     3661 ttaaagatgt acttttgcaa tgaattttta ataatttacg gggttatgga gaatttagac
     3721 tggaaaaacc tttcattcgg ctacatcaag actgattata atatccgttg tttctacaag
     3781 gacggcaagt ggggtgaact gattgtttcg gattctgaat acattgatat tcatattgct
     3841 gcaacagccc tgcattacgg ccaggaagcc tttgaggggc tcaaggctta catgggtaag
     3901 gacggcagga taaggctgtt ccgctgggaa gaga
//
LOCUS       DAOOYG010000186         3892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_27754_length_3892_cov_5.303101, whole genome shotgun sequence.
ACCESSION   DAOOYG010000186 DAOOYG010000000
VERSION     DAOOYG010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27754_length_3892_cov_5.303101"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="PK288_11175"
     CDS             complement(<1..1669)
                     /locus_tag="PK288_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007216484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPM19532.1"
                     /translation="MLYPNEFPLQDVVLLDGPFKHARDLNLRTLLKYDIDRILEPFLR
                     EAGLQPRGEMYPNWEGLAGHIGGHYLSAMAMNYAATGNTECKRRMEYMISELRACQEA
                     NGINHPEWGTGYVGGVPGSNELWSTLQKGDFSVYRTKWVPWYNVHKLYAGLRDAWSYT
                     GNEEARAIFLRLCDWAINITSALSDEQMESMLDMEHGGINETLADAFRMTGDEKYLTA
                     AMRFSHRMLLDPMSAGIDNLDNKHSNTQIPKAVGFQRIAELSGNRKYADAGQFFWETV
                     TGNRSLAFGGNSRREHFPEAAACIDYTEDIEGPESCNSYNMLKLTEFLFRTNPSARYT
                     DYYERTLLNHILSAQHPEHGGYVYFTPVRPRHYRVYSAPNQAMWCCVGTGMENHGKYN
                     QFIYTHRHDSLFLNLFIASELTWKERGIKIRQETLFPYEEGTSLEVIDGSGRFSLMVR
                     YPGWVKEGELTILLNGKPVPFEANPSSYVAIDRRWKKGDKIEVIFPMHNRTEQLVNVP
                     GYVAFMHGPVLLGTKTGTEDLKGIIADDSRWGHIPSGQKFPVDEAPIL"
     gene            complement(1780..2322)
                     /locus_tag="PK288_11180"
     CDS             complement(1780..2322)
                     /locus_tag="PK288_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HPM19533.1"
                     /translation="MRSEQFTGVFSAMLMAALMLIPWDLGAQEQQKQRVIILTDVENE
                     PDDTQSLIRLLLYSNLPEIKGIIATTSVHMKKEVHPESIRKAIRAYGMVQANLAKHEA
                     GFPVADSLMLLVKQGLPEYGMNAVGDGRDSEGSDWIIKTLQSAILSFIFSIAPEAQSI
                     VVSPGPGGTRGGTPLKKAEI"
     gene            complement(2583..3419)
                     /locus_tag="PK288_11185"
     CDS             complement(2583..3419)
                     /locus_tag="PK288_11185"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPM19534.1"
                     /translation="MKIDAHQHFWIYNSSEYGWIGHNMEILKRNYTPEDLCNELIKAG
                     YSGSVAVQARQSVEETRWLLELAGKHDFIRGVVGWADLSGKDDLRRQLDDFCTNEKFV
                     GLRHVIHDEPDDNFMLRDDFLKGISILNEYDLTFDLLLFPKHLPVAQLLVSLFPEQKF
                     VLDHISKPLIRNNVVSPWREDITRLAQNENVWCKLSGMVTEAESKNCDHEDFKPYLDV
                     VFEAFGAERLMIGSGWPVCRLAGEYQDVVGIVEEYISGMSDDLRTGITGENCIIFYGL
                     ER"
     gene            complement(3416..>3892)
                     /locus_tag="PK288_11190"
     CDS             complement(3416..>3892)
                     /locus_tag="PK288_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPM19535.1"
                     /translation="VDILDERLAIAQKAYNDIYIVNAANTNAVEKIRELTGGEGVDIA
                     FEAVGHTKEIPGVINPVRGCVQAIRGAGKVCVLGLSSEPSPVVMKELILKEGIIMTSR
                     VSHGEFAETIENMSRYTLKPEAVVTDIMHPSETQEAFEMLEKEPHKHLKIMLKFSE"
BASE COUNT          963 a          987 c          856 g         1086 t
ORIGIN      
        1 tcaatatcgg agcttcatca acaggaaact tttgccctga ggggatgtgt ccccatcggc
       61 tgtcatccgc tattatccct ttaagatctt ctgttcctgt ttttgttccc agcaggacag
      121 gcccatgcat gaacgcaaca taaccgggaa catttaccag ctgttccgtt ctgttgtgca
      181 tcgggaaaat tacctctatt ttgtcaccct ttttccacct acggtctatc gcaacataag
      241 aagagggatt ggcctcaaac ggaacgggtt tcccattcag gaggatcgtc agttcgccct
      301 ccttaaccca tcccggataa cgaaccatta atgaaaacct gcccgatcca tcgataactt
      361 ccaggctggt tccttcttca tatgggaaga gggtttcctg ccggatcttt atacctcttt
      421 ccttccatgt cagctctgag gcaatgaaaa gattcaggaa caaggaatcg tgcctgtgtg
      481 tgtagatgaa ctggttgtat ttcccgtgat tctccatgcc ggtgcccaca cagcaccaca
      541 ttgcctggtt gggagcagag taaaccctgt aatgacgggg gcggacagga gtgaaataaa
      601 catagccgcc atgttcagga tgctgggcag acagaatgtg attaagcaaa gttctttcgt
      661 aataatctgt atatctggca ctagggtttg tcctgaagag gaattccgtc agcttgagca
      721 tattatatga attgcaggac tccggacctt cgatatcttc ggtgtaatct atacaggcag
      781 ccgcttccgg gaagtgttcc ctccggctgt tccctccgaa cgccagggag cggttgccag
      841 tcacggtttc ccagaagaac tgccctgcgt ccgcatactt cctgttgcca gagagttcgg
      901 caattctctg gaatcccact gccttcggga tctgggtatt tgaatgcttg ttatcgaggt
      961 tgtcgatacc tgccgacatc ggatcaagca gcatcctgtg tgaaaatctc attgcagcag
     1021 tcaggtattt ctcatctcct gtcatccgga aagcatcggc cagagtttcg tttatcccgc
     1081 catgctccat gtcaagcatt gattccatct gttcatcgga aagtgctgag gttatgttaa
     1141 ttgcccagtc gcataacctg agaaatatcg ccctggcctc ctcgttgcct gtatatgacc
     1201 atgcatccct gagcccggca tatagtttat gaacattgta ccacggaacc catttggtgc
     1261 ggtaaactga gaaatcgcct ttctgcagtg tgctccacaa ttcattgctg ccgggcacac
     1321 caccaacata tccggttccc cattcagggt gatttatgcc gtttgcctcc tgacatgccc
     1381 tgagttccga gatcatgtat tccatccttc ttttgcactc agtattcccg gttgcggcat
     1441 aattcatcgc catggctgac aggtagtgtc cgcctatatg acctgccaga ccctcccagt
     1501 tcggatacat ttcgcccctg ggctgtagcc cggcttccct cagaaatggt tcaagaatgc
     1561 gatcgatgtc atactttagc agggtccgga gattcagatc acgggcgtgt ttaaacggac
     1621 cgtcaagcag cacgacatcc tgcaagggga attcattcgg atacagcatc tgctgtgatc
     1681 tctgcctgca accggccaaa agaccacata acaggatcag gcagagggga gaggttctta
     1741 tcagcatacc ggttcgccgg tatgggagga tgtgaatttt catatttcag cttttttgag
     1801 aggtgttcca cccctggtac cacccggtcc gggactcaca acaatactct gcgcctccgg
     1861 cgctatgctg aagataaagg acaggatagc ggactgaagt gtttttatta tccagtcaga
     1921 tccttctgag tccctgccgt caccaactgc gttcatcccg tactccggca gaccctgttt
     1981 aacaagaagc atcaacgaat ccgccacagg gaaaccagct tcatgctttg caagatttgc
     2041 ctgaaccatc ccgtatgcac gaatggcctt cctgatagat tcagggtgaa cttctttttt
     2101 catgtgaaca gaggtcgttg caattattcc ctttatctcg ggtaaattgg aatacaaaag
     2161 caaccgtatg agagactgtg tatcatcagg ttcattttca acatctgtca ggatgattac
     2221 cctctgcttc tgttgttcct gtgcgccaag atcccagggg ataagcataa gcgctgccat
     2281 caacattgcg gagaatactc ctgtgaattg ctctgaccgc atattatatt gatttagctc
     2341 tgttcataat aagatgaacc gggaaatcac ggtaattgta aaactcataa aaattaattg
     2401 aagacaggtt cattttatat gcaaatgatg actgaaattt aaaagtcgat gataaaagag
     2461 ttaaaataat ttaattcccc gtgaatgata aaccggcatg atgaaggcac aagggaatgt
     2521 aatggaatct gccgcgtaac atgcagactc tccggacttt aggttttcga ccgtgcaacc
     2581 tgtcatcttt ccagcccgta aaaaatgatg caattttctc ctgtgatacc ggtacggaga
     2641 tcatccgaca ttccagagat atactcttcg acaatgccaa caacgtcctg atattctcct
     2701 gccagcctgc atacgggcca gccgcttccg atcataagcc tttcagcgcc aaatgcttca
     2761 aagacaacat cgagatatgg tttgaagtct tcatgatcac agtttttcga ttcagcctcc
     2821 gtgaccattc ccgacaactt gcaccagaca ttttcattct gcgccaaacg tgtgatgtct
     2881 tcacgccacg gactgacaac attgtttctt atcagtggtt tggagatgtg atcaaggaca
     2941 aatttctgtt cgggaaaaag tgacacaaga agctgtgcaa caggcaaatg cttgggaaaa
     3001 aggagaaggt cgaatgtgag gtcatactca ttcaggatgc tgataccttt caaaaagtcg
     3061 tcgcgaagca tgaaattgtc atcaggttcg tcatgaatga catgtctcag tccgacgaac
     3121 ttttcatttg tgcagaaatc atccagttgc cgcctcaaat catctttgcc tgacaggtca
     3181 gcccagccaa ctactcccct gatgaaatca tgttttcctg ccagctccag cagccatcgg
     3241 gtttcttcaa cactctgccg ggcctggaca gccactgatc ccgaatatcc ggctttgata
     3301 agctcattgc ataggtcttc cggagtatag ttgcgcttca gtatttccat attgtggcct
     3361 atccagccgt attcgctgct gttgtagatc cagaagtgct gatgtgcatc tattttcatt
     3421 cactgaactt caacatgatt ttcagatgct tatgaggctc cttttcaagc atttcaaatg
     3481 cctcctgtgt ttcggaagga tgcattatat cagttacaac cgcttcaggc ttaagtgtgt
     3541 acctgctcat gttttcgata gtttcggcaa attcaccgtg agaaacccgg gaggtcatta
     3601 tgatcccttc tttcaggata agttctttca tcacgacggg agatggttca gatgaaagtc
     3661 ccagtacaca tactttgcct gcccccctga ttgcctgtac acatcctctc acggggttga
     3721 tcacccccgg tatctctttt gtatggccca cggcctcaaa agcgatatcc actccttccc
     3781 ctccggtaag ttcccttatc ttttcaacgg cattcgtatt agctgcgtta acaatgtaaa
     3841 tatcattata tgctttttgg gcaatggcaa gacgttcatc aagtatatcg ac
//
LOCUS       DAOOYG010000187         3809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_28587_length_3809_cov_5.840703, whole genome shotgun sequence.
ACCESSION   DAOOYG010000187 DAOOYG010000000
VERSION     DAOOYG010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28587_length_3809_cov_5.840703"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2724
                     /locus_tag="PK288_11195"
     CDS             <1..2724
                     /locus_tag="PK288_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPM19536.1"
                     /translation="SIRSDEALQGRAAGVVVTNNDGAPGGAATIRIRGGNSITGSNNA
                     LVVIDGFQGGDLSSLNPNEVESIEVLKDASATAIYGSKGANGVILVTTKRGKTGAPVF
                     DYSYSYGIQSIVKKIDLFNAGEYARIANIWKASQNLYGDPVLPFTEAQIAELERTGGT
                     DWQDEMFRKAPLQMHQISIRGGSESVRYFIAGGYFDQTGLIVNTQFERYTLRSNLDMN
                     LSKWLRAGLNMNVIKDRGNIPPSGEGTRYVDILTQAVNAVLRFDPITPVFDELGNYSR
                     PPSTYGDRDVWNPMATAMGSFNELSNMTTNMNSFLEFTILEGLTFRVTGAAGINNSDK
                     KQYYNTLTKMGRPINGIGNLIDDKSLYYQNSNILTYDKIINDRHHITLTGVAEQQSTK
                     SYGSDIQAQGFASDITGVYDIGGASQTNYRSSYDSRRSINSWLVRVNYGFADKYLLTV
                     SYRADGSSVFGKNNKWGYFPSASIAWRASEEEFIKNLNIFSDLKLRGSWGKTGNQAIA
                     VYGSLAKIGSGLNYLYNGTSITDIGYGITSAANVNLKWETTAQTDLGIDMGLFKGRLT
                     ATVDIYRKETTDLLLNKPVPFYTGAPSAQLLDNVGSIENKGLEITVGGDPVVGAFRWN
                     TGVNITINRSKVLKLVNELPMGLRTNTGGGYQIYNATSNSLMYLQEGQPMGQMRGYIV
                     LGTWGESERAEALKYGQLPGDQKYKDMNNDGKITEAGDVTVIGNSSPDFVFGWNNSMA
                     WKNFTLSFLIQGSQGNDIFNATRIQIEAPSKGTSKALNDRWTIDNQDTDIPAFTDQIT
                     RREALLGIPSKISLGRSPNRLSRYVEDGSYARLKTVTLGYNLPKTIIGKIGLTNLRAY
                     VTAANIITLTKYSGYDPEVSSFNVATDGGRGIDLSNYPTVKTITFGINLTF"
     gene            2729..>3809
                     /locus_tag="PK288_11200"
     CDS             2729..>3809
                     /locus_tag="PK288_11200"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM19537.1"
                     /translation="MKKTYRNISIWSIIILLIMTGVSCLKLDEEPKGLSTPDNFYTTP
                     GQCEAAFAASMNTLYDTWSGYENVYGAFPDGQYTDVSLDYGPNYNSMYWQIHYRAIND
                     INAVLKAVIKRNSLGAADPETVKNIIAQAKFLRAFNYFTLVRLWGKVPFLTENSPDPI
                     SNPLKPESRLEIAALYDSLEADLDFAIENLEDYDASRPARPNIWLAKTLLAKVYITRA
                     TNPLNETSYYAKARDLADDIILHGPYTLLPKFEDVFLSSNKNNSEIIWAFQTTDDDPN
                     IDGIAMAPSEWGGWSGGAVKPRWAEEYPDQPRKKGYILMDFPSDITTDPMGPIINYKE
                     SIDGTPYIQKYLWPMITLEEQLNISR"
BASE COUNT         1179 a          792 c          898 g          940 t
ORIGIN      
        1 tctatcaggt cagatgaagc cttacaggga cgtgccgccg gtgtggttgt cacaaataat
       61 gacggggctc cgggcggagc agcaacaatc aggatccgtg gaggtaactc gataaccgga
      121 agcaacaatg cacttgttgt aatagacgga ttccagggag gcgatctcag ctctttgaat
      181 cccaatgagg tggaatcaat agaagtactt aaggatgcct cggctacagc tatttacggc
      241 tctaaaggcg ccaacggagt aattcttgtt acaaccaaaa gaggaaaaac aggcgctcct
      301 gtctttgatt acagctatag ctacggaatt cagtccatag taaagaaaat cgatcttttc
      361 aatgcgggtg aatatgcccg gatagccaat atatggaaag catcccagaa tctgtacggc
      421 gaccctgtat tacctttcac cgaagcacag attgccgagc tggaaaggac aggaggaact
      481 gactggcagg acgaaatgtt ccggaaagca cctttgcaga tgcaccagat ttcaataaga
      541 ggaggatcgg aatcagtcag atacttcatt gcaggtggat attttgatca gacaggattg
      601 atcgtcaata cccagttcga acgttatacc ctgaggtcaa acctggatat gaacctttca
      661 aaatggctga gagcaggcct gaatatgaat gtaatcaaag acaggggcaa tataccacca
      721 tccggtgagg gtacccgata tgtcgacatt cttacacagg ccgttaatgc cgttcttcgc
      781 tttgatccga taacaccggt ctttgatgag cttggtaatt acagcagacc tccaagtaca
      841 tatggcgaca gggatgtgtg gaacccgatg gctactgcta tgggatcttt caacgagctg
      901 agcaatatga caaccaatat gaactcattt ctggaattca cgatcctcga ggggcttact
      961 ttcagagtta ccggcgctgc aggaattaat aattctgaca aaaaacaata ttacaatacc
     1021 ctgacaaaaa tgggacgacc tattaatgga attggtaacc tgatagatga taaaagcctt
     1081 tattaccaga attccaatat tcttacctat gataaaatta taaatgacag gcatcatatt
     1141 actctgacgg gagtggctga acaacagtca acgaagagct atggttcaga tatccaggct
     1201 cagggatttg cctcggatat tacaggagta tatgatatcg gcggagcaag ccagactaac
     1261 tacaggtcat cctatgattc aaggagaagt attaattcat ggctggtccg tgtcaactat
     1321 gggtttgcgg ataaatatct tcttacagtc agctacaggg ctgacggatc atcagtgttt
     1381 ggaaagaata ataagtgggg gtatttcccg tcagcatcaa tagcctggag agcatccgaa
     1441 gaagagttca ttaaaaatct taacatattt tcggatctta aactccgggg cagctggggt
     1501 aaaaccggta atcaggcaat tgctgtttac ggttccctgg ccaagatcgg atcgggattg
     1561 aactatcttt acaatgggac tagtataacg gatattggtt atggaataac aagcgctgct
     1621 aatgtaaacc tcaaatggga gaccactgca cagactgatc tggggattga catgggcttg
     1681 tttaagggca ggcttactgc cacggtagat atttacagaa aggaaactac cgatctgttg
     1741 ttgaacaaac cggtaccttt ctataccggg gcgccaagtg ctcagttact ggataatgtc
     1801 ggatcaattg aaaacaaagg ccttgaaatt actgtcggcg gcgatcctgt agtcggtgca
     1861 ttcagatgga ataccggggt aaatattaca ataaacagga gcaaggttct taagcttgta
     1921 aatgaactgc caatggggct gagaacaaat accggaggcg ggtaccagat ctacaatgca
     1981 acatccaact ccctcatgta tctgcaggaa ggccagccaa tgggtcagat gcgaggatat
     2041 attgttctgg gaacatgggg agaaagtgaa cgggccgaag ccctgaagta tggccagctg
     2101 ccaggcgatc agaaatataa ggatatgaac aatgacggaa agatcactga agccggcgat
     2161 gtgaccgtga taggaaattc atcacctgat ttcgttttcg gatggaataa cagcatggca
     2221 tggaaaaact tcacactatc cttccttatt cagggatcac agggtaatga catattcaat
     2281 gcaacaagaa tacagataga agcgccgagc aaaggaacaa gcaaggcttt gaatgacaga
     2341 tggacaattg ataatcagga tactgatatc ccggcattta ccgaccagat aacacgacgc
     2401 gaggcccttc tggggatacc atcaaagata agcctcggga ggtcgcctaa cagactgagc
     2461 agatatgttg aagacggatc atatgcccgg ctgaaaacag taactctggg atataatctc
     2521 ccgaaaacca ttatcggtaa gattggtctt acaaacctca gggcatatgt tacagctgcc
     2581 aacatcatta cccttacaaa gtattccggc tatgatccgg aggtaagttc ctttaacgtt
     2641 gctaccgacg ggggaagagg tatagacctt agtaattacc ccactgtaaa aacaatcaca
     2701 ttcggaatca atttaacctt ctgagaaaat gaaaaaaaca tatcggaata tttcaatatg
     2761 gagcattatt atattgctca ttatgacggg agtatcgtgt ctgaaacttg atgaggaacc
     2821 aaaagggtta tcaacccctg ataattttta tacgactcct ggtcagtgtg aagccgcttt
     2881 cgctgcatcc atgaataccc tttatgacac atggagtggt tatgaaaatg tatacggcgc
     2941 ttttcccgac ggacaatata cggacgtcag ccttgactat ggccctaatt ataattcaat
     3001 gtactggcag atccattaca gggcgatcaa tgatataaat gccgtcttaa aagcagtaat
     3061 aaaacgtaac agtctggggg ctgctgatcc cgagacagtt aagaatatca tcgctcaggc
     3121 taagtttctc agggcattca attactttac cctggtcagg ttgtggggaa aggttccgtt
     3181 cttaacagaa aattctcctg atcccatcag taatccgctt aaacccgaat cacgtctgga
     3241 gatcgcagct ttgtatgatt cccttgaagc agatctggat tttgctatag aaaatcttga
     3301 agattatgat gcttcgcgcc cggcacgccc gaatatctgg ctggcaaaaa cattgcttgc
     3361 aaaagtctat ataacaaggg caacgaatcc gcttaatgaa acatcctatt atgcaaaagc
     3421 cagggatctg gctgatgata taattcttca cggaccatat acgcttcttc caaaattcga
     3481 agatgttttc ctcagttcca ataaaaataa cagcgagata atctgggctt ttcagacaac
     3541 agatgatgat ccgaatattg acgggatagc catggcgccg tcagaatggg gaggatggag
     3601 cggaggagct gtcaaaccgc gctgggcgga agaatatccg gatcaacccc gtaaaaaagg
     3661 ttatattttg atggattttc cgtcggatat tacaactgat cctatgggac cgatcatcaa
     3721 ctataaggag tcgatagacg gtacaccata tatccagaaa tacctgtggc cgatgataac
     3781 acttgaagaa cagcttaata tatctcggc
//
LOCUS       DAOOYG010000188         3803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_28649_length_3803_cov_6.349520, whole genome shotgun sequence.
ACCESSION   DAOOYG010000188 DAOOYG010000000
VERSION     DAOOYG010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28649_length_3803_cov_6.349520"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="PK288_11205"
     CDS             complement(<1..160)
                     /locus_tag="PK288_11205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPM19538.1"
                     /translation="MKKAEIHTARGVMHVDFFEDDAPATVSNFVKLAREGFYDGLAFH
                     RVIPGFVIQ"
     gene            complement(162..875)
                     /locus_tag="PK288_11210"
     CDS             complement(162..875)
                     /locus_tag="PK288_11210"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPM19539.1"
                     /translation="MKINGLIVAAIMVALMSGSCAKNAISNAKMKNRTDSLSYAFGIV
                     NYNALKNDSLNLEPRLVAKAMLDGEKGRPVMSDDAARSFIMSFISDRENQKAEKQAAA
                     NKVLYKDHIDQNVAFLENNKTKPGIIVTPSGLQYEVIRMGTGEKPTRDSQVKVHYVGT
                     LIDGKEFDSSVKRNEPAQFPVTGVIPGWTEALQLMPVGSKFRLFIPENLAYGARGAGD
                     IIMPYSTLIFEVELLEIVH"
     gene            complement(950..1609)
                     /gene="mnmD"
                     /locus_tag="PK288_11215"
     CDS             complement(950..1609)
                     /gene="mnmD"
                     /locus_tag="PK288_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HPM19540.1"
                     /translation="MQYIIAKTGDGSHTLYVPEIDEYYHSIHGAIQESEYVFIKCGQE
                     FSSANPLRIFEAGFGTGLNALLACISAEAGRRTVFYTSIEKYPLPERIITSLNHTAML
                     GEEAKHWFELIHSCEWNRPEEITKNFILHKICGDLITDDLEGFYDLIFFDAFSPGRQP
                     DIWAPELFRKISRITVPGGIFMTYSVKGEIKRALRANGFRVTLLPGPAGKRHILRAFK
                     S"
     gene            complement(1711..2310)
                     /locus_tag="PK288_11220"
     CDS             complement(1711..2310)
                     /locus_tag="PK288_11220"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPM19541.1"
                     /translation="MKYDIKPGVKGLIFDLDGTLADTMPYHFRAWQRACARYGAHIDN
                     KFLRRHTGSPGWIIAEEVIRSCGLNGSVSIDSIVNEKMKEFLRLQHLIKPIEPVTDIV
                     RKYHGILPMAVGTGGHREAVEKTLEITGLRHFFEIIITANDVSSFKPHPETFLRCAEL
                     MHVDPPFIEVFEDGELGIEAAQNAGMMVTDVRSWYDSDW"
     gene            complement(2359..3426)
                     /locus_tag="PK288_11225"
     CDS             complement(2359..3426)
                     /locus_tag="PK288_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19542.1"
                     /translation="MKKFILALLFPAIFLVQPLSGQSDSLIFQAAVSDTQDVSVRLFD
                     SDKPLDISLRFDISQYKRKRSDTTYLNAVLTYYTDENDSVSKKIKIRARGEIRRKICD
                     FPPFFLNFRMKDSKDGEFVGINKLKVVPYCKLGYEDYVLREYLIYKLYNVLTENSLRV
                     RLFRINLYNTAKPGKPLKQYGFAIEPVRVFEKRTGSFELKNVKLSRSFIKPEMLDRCA
                     IFNYMIGNTDWSIPNMHNVHLFQQPFSEQPELAMIVPYDFDYSGLINAEYAVPFETLP
                     IKSVKERLYLGSCRSREEFRNALDEFIARKDEFYRTVNDFAYLSDRSKREIIRYLDGF
                     YRLFDKRETIIATLLLECREP"
     gene            complement(3602..>3803)
                     /locus_tag="PK288_11230"
     CDS             complement(3602..>3803)
                     /locus_tag="PK288_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007367122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19543.1"
                     /translation="PDNIPVFGLTAGLPVTLAGLAGSLGRVWGVSSFGFSAPYKVLDE
                     KLGFTADNVYRQVMDFLALNRK"
BASE COUNT          982 a          881 c          859 g         1081 t
ORIGIN      
        1 cctggataac aaatccgggt atgaccctgt gaaaggcgag gccgtcataa aatccttccc
       61 gtgcgagctt aacaaaatta ctgacggttg cgggcgcatc atcctcgaaa aagtcgacat
      121 gcatcacacc ccttgcagta tgtatttcag cctttttcat tctagtgaac gatttcgaga
      181 agttccactt caaaaataag ggttgaatag ggcattatta tatcaccggc cccgcgggct
      241 ccataggcaa ggttttccgg aatgaaaagc ctgaattttg aaccgacggg cattaactgc
      301 aaggcttcag tccagccggg aatcaccccg gtgaccggaa actgtgccgg ctcattcctc
      361 ttgacggagg aatcaaattc tttcccgtca ataagggtgc ctacataatg aaccttgacc
      421 tggctgtccc ttgtaggctt ctcaccggta cccatcctga tgacctcgta ttgaagaccc
      481 gaaggagtaa caattattcc cggctttgtc ttgttatttt ccagaaaggc tacattttga
      541 tcaatatggt ccttgtacaa aaccttgttg gctgcagctt gcttttcagc tttctgattt
      601 tcacgatcgc tgatgaacga catgatgaaa cttcttgcgg catcgtcact catcaccggc
      661 cttccctttt ccccgtcgag catcgccttt gcaacaagcc tgggctccag gttcaggctg
      721 tcatttttca gggcattgta gttaacaata ccgaaagcgt atgaaagcga atcggttctg
      781 tttttcattt tagcattcga tattgcattt tttgcacacg agcctgacat cagggcaacc
      841 atgatggccg cgacgatcaa tccattgatt ttcatttgaa gtagttttgg ttaaaatttt
      901 ggcaaagata atattttgac tgcacgaaca aagtaaatgg gtcttttttt caggatttaa
      961 atgctcttag aatatggcgc ttgcctgccg ggccggggag taatgtaacc cgaaatccgt
     1021 ttgccctgag agctctcttt atctcacctt ttaccgaata tgtcataaag atacctcctg
     1081 gcaccgttat ccgtgaaatc ttcctgaaaa gttcgggtgc ccatatatcc ggctgtctgc
     1141 ctggactgaa ggcatcaaaa aagataagat cataaaaccc ttccaggtca tcagtgatca
     1201 ggtcaccaca gatcttatga aggatgaagt ttttggttat ttcctccggc cggttccatt
     1261 cacaggagtg aatcagttca aaccagtgct ttgcctcttc accaagcata gcggtatggt
     1321 taagtgaggt gatgattctt tcggggagag gatacttctc aatcgatgtg taaaataccg
     1381 ttcttctgcc ggcttctgca ctgatgcagg ccagaagggc attcagaccc gttccgaatc
     1441 cggcctcgaa gatcctgagc ggatttgctg atgaaaattc ctgcccgcat ttaataaaca
     1501 catattctga ctcctgaatg gcgccgtgga tggaatggta atattcatca atttccggaa
     1561 cataaagagt atgcgatccg tctccggttt ttgcaatgat atattgcata atctgaatga
     1621 tcttagtgaa tactgatgct gataaaaggg tttggcatgg aatgcggaaa agaagtgaca
     1681 atcattcagc ggtaaccgct cagtattccc ttaccagtcg gaatcatacc aggatctgac
     1741 gtcggttacc atcataccgg cgttttgcgc agcttcaatg ccaagttcgc catcttcgaa
     1801 aacttcaatg aaaggcggat caacatgcat aagctccgca catctcagga atgtttcagg
     1861 atggggtttg aatgaactca catcgtttgc ggtgatgatt atttcgaaaa aatgtctcag
     1921 tccggttatt tccagtgtct tctcaactgc ctccctgtga cctcccgttc ctacagccat
     1981 gggcaggatc ccatgatatt tccttacgat atccgtaaca ggttcaatgg gttttataag
     2041 atgctggaga cggagaaatt ccttcatttt ttcgttcact atgctgtcga tggatacact
     2101 gccgttgagt ccgcagcttc tgatcacttc ttctgcaatt atccagcctg ggctgccggt
     2161 atgccttcgc aggaatttat tgtcaatatg ggctccgtac ctggcacagg ccctttgcca
     2221 ggccctgaaa tggtacggca tggtgtcggc aagggttccg tcgagatcaa agatcaatcc
     2281 ttttacaccc ggtttgatat cgtacttcat gggaagcttg ttaatggagg tgcaaaaata
     2341 gtgaaaaaaa tgacatgatc agggttccct gcattctaga agcagggtgg caattattgt
     2401 ttcccgttta tcgaaaagcc tgtagaatcc gtcgaggtat ctgattattt ccctttttga
     2461 cctgtcgctc agataggcga aatcatttac tgtccggtaa aactcgtcct ttctggcgat
     2521 aaattcatcc agtgcattcc tgaattcttc cctgctcctg catgagccaa gatataacct
     2581 ttccttcacc gatttgattg gcagtgtttc aaagggaacc gcatattcag cattaatcag
     2641 tccggagtag tcaaaatcgt agggaacgat cattgccagt tccggctgtt ccgagaatgg
     2701 ctgctgaaac aggtggacat tgtgcatgtt agggatcgac cagtcggtgt tccctatcat
     2761 atagttgaag atcgcacatc tgtccagcat ctccggcttg ataaaacttc ttgacagttt
     2821 gacattctta agttcaaagg aaccggttct tttctcaaat accctgacag gctcaatagc
     2881 aaacccatat tgtttcaatg gtttgccggg ctttgcggtg ttgtacaggt ttatcctgaa
     2941 aagacgtacc ctcagactgt tttcggtaag gacgttatat agcttgtaga tcaggtattc
     3001 cctgagtaca taatcctcat atcccagttt gcagtagggt acaaccttga gcttgtttat
     3061 accaacgaat tccccgtcct ttgagtcttt catcctgaaa ttcaagaaaa acggaggaaa
     3121 gtcacatatt ttccttcgga tctcgcccct ggcccggatc tttatttttt tactgactga
     3181 atcattttca tcggtataat atgtgagaac ggcattaaga tatgtggtgt cggacctttt
     3241 cctcttatat tggcttatat cgaacctgag tgagatatcc agcggcttat ccgagtcaaa
     3301 aagcctgaca ctgacatcct gcgtatcgct gacggctgcc tgaaagatca gggaatcgct
     3361 ctggcctgaa agaggctgaa caagaaaaat ggcaggaaaa agcagggcta aaatgaattt
     3421 tttcatacag gtaagattgg gttagcggaa agtaatacag ctgatgattt taaaccaaga
     3481 taaacaaaac aggaaatccg ggacagcatg tttttaacat tatgctgtat tattcaacaa
     3541 catgttgcac gcagccggcc aggtatccgg atgcggcata tggttgtgac cggtagaatg
     3601 gttatttcct gttcagtgcc aggaaatcca tgacctggcg gtatacatta tcagctgtaa
     3661 agccaagctt ttcgtccagt accttgtaag gagcggagaa gccgaaactg ctgacacccc
     3721 acaccctgcc gagggacccg gcaagtccgg caagcgtcac aggcaggcct gccgtgagcc
     3781 cgaataccgg aatattgtcg gga
//
LOCUS       DAOOYG010000189         3607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_30804_length_3607_cov_6.149212, whole genome shotgun sequence.
ACCESSION   DAOOYG010000189 DAOOYG010000000
VERSION     DAOOYG010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30804_length_3607_cov_6.149212"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..308)
                     /locus_tag="PK288_11235"
     tRNA            complement(236..308)
                     /locus_tag="PK288_11235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(273..275),aa:Arg,seq:ccg)
     gene            469..3129
                     /gene="leuS"
                     /locus_tag="PK288_11240"
     CDS             469..3129
                     /gene="leuS"
                     /locus_tag="PK288_11240"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPM19544.1"
                     /translation="MEYNFKQIEKKWQQYWEENKTFKTPEDLSNPRKCYVLDMFPYPS
                     GAGLHVGHPEGYTATDIYARYKRMKGFNVLHPMGWDAFGLPAEQYAIETGTHPAVTTR
                     KNCDTFRRQIKGLGLSYDWDKEINTTDPKYYKWTQWIFIRLYNTWFDEVLQKGRHISE
                     LTIPEDILSSGKEAVRKYTDSKRLAYYADALVWWCPDCRIVCSNEEVLNDGSHEKCGN
                     IVEKKNLKQWMLRIPLYAERLLKGIDNLDWPEGIKEMQRNWIGRSTGAEVDFKIEGME
                     ETLTVFTTRPDTLFGATYMVIAPEHVLVPAITMPGYKESVNEYIRVANLKSELDRTDL
                     AKEKTGVFTGRYAINPVNGKRIPIWIADYVLTGYGTGAIMAVPAHDTRDFEFARKFGL
                     PIVCIQEPDVEDRARRDRIINGDECWTEDGSYINSSDLSTGIDINGLNKEAGIARIIG
                     WLESKGLGKAKVNYKVRDWLFSRQRYWGEPFPVIHWEDGEISILDEKDLPLELPEMEK
                     FQPGETGESPLSNAVDWLEVTDQNGRRGRRETNTMPQWAGSCWYYLRFIDPDNDEQIF
                     DSEKEKYWMPVDLYIGGAEHAVLHLLYSRFWHKVLYDLEIVSTDEPYTRLFNQGMILA
                     FAYENEAGAKIPADQIEERDGRFYNGENGTEVRQIVAKMSKSLKNVVNPEDVTMRYGA
                     DSLRLYEMFMGPLDVMKPWDDRNVKGVFNFLVRTFRFFSNDANISGGPEDVEILKGLH
                     KTIRKVEEDIGTLKFNTAISEMMIFLNLVYKKGKVTRETARSFILILSPFAPHLGEEL
                     WQLLGHESTLAYESWPGVNEEYLKADIVEYPVSFNGKMRFKIEVPANLDRDEIARKVL
                     ADERAGKWLADKSVKNVIVVPDKIVNVVTR"
     gene            3147..>3607
                     /locus_tag="PK288_11245"
     CDS             3147..>3607
                     /locus_tag="PK288_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19545.1"
                     /translation="MANRILSIVFFIVILFTAACRPPSTTGDCSPDASYDTASAGSPA
                     DIPLMYGIPIDSFDLVSGEIKRNGFLSTLLPKYGITIQELEMVLKNSADVFDVRKIRF
                     GSNYTLFCDRDSNARARYMVYEHDPTTCYIFSFRDSLNITRYRKEINKEIR"
BASE COUNT         1058 a          785 c          914 g          850 t
ORIGIN      
        1 ggtggatgcg ataaacaaca acggctgcat aaaaccgatg attttacaca gccgccggtg
       61 gcagtgatcc cggagcgact gttcgctacc cacgggcaag cgctgaagct cgctcacggt
      121 gttcgctcga ttcgaacgcc gcctccctcc gcgttctcgt cgctcctgca tggtggatgc
      181 gataaacaac aacggctgca taaaaccgat gattttacac agccgcaggt ggcagtgatc
      241 ccggagcgac tcgaacgctc aaccaacaga accggaatct gtcattctat ccattgaact
      301 acgggacctg atattttacg gcaaaaataa aagctatgga cgggttttac aactatttgt
      361 caaagatttc ctattttcgc aggctgtaga aattaggatt aaatacggat gaccggcgcc
      421 ggcgccggtc atgggtcaga taaattgcga acgatacctg ttaatagaat ggaatacaat
      481 tttaaacaga ttgaaaaaaa atggcagcag tattgggagg aaaacaaaac cttcaaaact
      541 cctgaagacc ttagcaaccc aaggaaatgc tatgtcctcg acatgtttcc ctatccgtcg
      601 ggagcaggac tgcatgtcgg acatccggaa ggttacactg cgactgatat ttacgccagg
      661 tacaaaagga tgaaaggttt taatgtactg catccaatgg gatgggatgc attcggtctg
      721 ccggcggaac agtacgcaat tgagacgggc acccatcctg cagttacgac caggaaaaac
      781 tgtgacactt tcaggcggca gataaaggga ttaggactga gttatgactg ggacaaagaa
      841 atcaacacaa ccgaccctaa atattacaaa tggacccagt ggatatttat ccggctttat
      901 aatacctggt tcgatgaagt cctgcagaaa ggcaggcata tctcagaatt gacaataccc
      961 gaagatattc tttcatcggg taaagaagcg gtaagaaagt atacagattc aaaacggctt
     1021 gcttattatg ctgacgccct ggtatggtgg tgccctgact gccgtattgt ctgttccaac
     1081 gaggaagtgt taaatgacgg ctcacatgag aaatgcggaa acatcgttga aaaaaagaat
     1141 cttaagcagt ggatgctgag gattcccctt tatgctgaaa gactccttaa gggtatcgat
     1201 aatcttgact ggcccgaagg aattaaggaa atgcagagaa actggatcgg caggtcaact
     1261 ggagctgaag tcgactttaa aattgaggga atggaggaga ccctgacagt attcacaacg
     1321 cgtccggata ctttgttcgg ggccacatat atggttattg ctcccgaaca tgttctggta
     1381 cccgctatta ccatgcccgg gtataaagaa tccgtaaacg agtatatcag ggttgcaaac
     1441 ctcaaatcgg agcttgacag gacagatctg gcaaaagaaa agaccggagt ttttacaggc
     1501 agatatgcaa tcaatcccgt gaacgggaaa aggataccga tatggatagc tgattatgtt
     1561 ctcacaggtt acggaaccgg ggccataatg gctgttcctg cccatgatac aagggatttt
     1621 gaatttgcgc gcaaatttgg gctgcccata gtttgtatcc aggagcccga tgttgaagac
     1681 cgcgccagga gggaccggat cataaatggc gatgaatgct ggaccgaaga tggaagctac
     1741 atcaattcat ctgatctgtc aaccgggatt gacatcaacg gattgaacaa ggaggcaggc
     1801 attgcacgga tcatcggatg gctcgaatca aaaggcctgg gaaaggcaaa ggtcaattat
     1861 aaggttcgcg actggctgtt cagccgccag agatattggg gggagccgtt ccctgtaatt
     1921 cactgggaag acggtgaaat aagcatactc gatgaaaagg atcttcccct tgagctaccc
     1981 gaaatggaga aatttcagcc cggggaaacg ggggaatcgc ccctttcgaa tgcggtcgac
     2041 tggcttgaag taacggatca gaacggccgc aggggcagaa gggagacaaa cacaatgcct
     2101 caatgggcag ggtcgtgctg gtattatctg cgtttcatcg atcccgacaa cgatgaacag
     2161 atcttcgaca gtgaaaagga aaaatactgg atgcctgtcg atctttatat cggcggcgcc
     2221 gagcatgctg ttctccatct gctctacagc cggttttggc acaaggtgct gtatgacctt
     2281 gaaatagtat ccactgacga gccctatacc aggctgttca accagggtat gatcctggcc
     2341 tttgcctatg aaaatgaggc cggcgccaag ataccggccg atcagattga agaaagggac
     2401 ggcagattct acaatggcga aaacggaacc gaagtgcggc agatagttgc caagatgtca
     2461 aaatcgctca agaacgtggt caaccccgaa gatgttacca tgaggtatgg ggccgattcc
     2521 ctgcggctct atgaaatgtt catgggtccg cttgacgtga tgaaaccatg ggacgaccgg
     2581 aatgtcaaag gggtgttcaa cttcctggtc agaaccttca gatttttttc aaatgatgcc
     2641 aacatttccg gggggccgga agatgttgaa atcctgaaag gacttcataa gacaatcagg
     2701 aaagttgagg aagatatagg aaccctgaaa ttcaatactg caatatcaga aatgatgata
     2761 ttccttaatc ttgtttacaa gaaaggaaaa gtgacaaggg aaacagcccg cagctttatt
     2821 ctgatacttt caccctttgc tccccacctt ggagaggaac tctggcagct gctcggccat
     2881 gaatcaaccc ttgcatatga atcctggccg ggtgtgaatg aagaatacct gaaggccgac
     2941 atagtggaat atccggtttc attcaacggg aaaatgaggt ttaaaattga ggtgcctgcc
     3001 aatcttgaca gagatgagat agcaagaaag gtccttgcag atgaaagagc cgggaaatgg
     3061 ctggcagata aatccgttaa gaatgtgatt gtggtacctg acaaaatagt taatgttgta
     3121 accagatagc cggttctcat cttattatgg cgaacaggat actaagtatt gtttttttta
     3181 ttgttatttt attcacagca gcctgcaggc cgccctcaac aacaggtgat tgcagcccgg
     3241 atgcatcata cgacactgct tctgccggga gtccggcaga tatcccgttg atgtacggaa
     3301 tccccataga ttcatttgat cttgtttcgg gagaaataaa gcgtaacgga tttctttcca
     3361 ccctccttcc caaatatgga attaccattc aggaacttga aatggttctg aaaaattccg
     3421 ctgatgtttt cgatgtcaga aaaataaggt ttggcagcaa ttacacgctt ttctgcgaca
     3481 gggacagcaa tgcccgggcc cggtacatgg tttatgaaca tgatcccaca acttgctata
     3541 ttttcagctt ccgtgactcg ctcaatataa cccggtacag gaaggaaata aataaagaga
     3601 tcaggtt
//
LOCUS       DAOOYG010000190         3543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_31573_length_3543_cov_9.338016, whole genome shotgun sequence.
ACCESSION   DAOOYG010000190 DAOOYG010000000
VERSION     DAOOYG010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31573_length_3543_cov_9.338016"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..897
                     /locus_tag="PK288_11250"
     CDS             187..897
                     /locus_tag="PK288_11250"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19546.1"
                     /translation="MNSSSWFSTQSLAWGLFSYMKWAETLPEGNTTSSKISITLNGKK
                     TEKTIRAKELMTEDLEMTSPDNPITVINNSEAPVYVTLVRKGISMLPDVSSEEKGLSI
                     KVGYINMNMNTIDHKSLKMGTDFMMVVNVTNNTFSEVNNIALTTMVPSGWEIRNTRLF
                     EARYGIKQSAYDYTDIRDDRVNTYFTLGRDETKTFVLVLNAAYKGEYYQPAIWCEAMY
                     TENCYARYPGTRVTVTGE"
     gene            900..3239
                     /gene="pbpC"
                     /locus_tag="PK288_11255"
     CDS             900..3239
                     /gene="pbpC"
                     /locus_tag="PK288_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPM19547.1"
                     /translation="MKRKFRIIIVIAGVMITPVLISPMPRFGAPLSTVVLAGDGSLLG
                     ARIADDGQWRFPGPDEVPEKFEKALLTFEDRYFYFHPGINPVSIIRALVRNIKAGDIV
                     SGGSTITMQVARLSGGNKSRTYAAKLSEMLSALKLEIFRTKKGILKTWSMNAPFGGNI
                     VGLEAASRRYLEKPAAEITWAEAAGLAVLPNSPSLVFPGRNQHILRKRRDDLLKKLYE
                     RKYFDSITYILALDEPLPGEPKPLPSIAPHLTDYFFSSNNGEMIRTTIDAALQARANA
                     IIESHSRQLSGNLINNAACLIVKVETGEVLAYVGNSRTDKPGNHGEDVDIIRSPRSTG
                     SILKPILYAGMQQSGDILPNTLVADIPTRFPGFTPENFDQSFSGAVPASLALSQSLNI
                     PAVRMLRKYSVEKLLDLFQKTGFTTFTRSADHYGLSLILGGGEATLWELTGVYASLSR
                     VLNRFGKEGKYCPGDYHMPVLMTGSINDNERPVYGEPVLSASSIWLTYEALKRVNRPE
                     AEAGWQFFSSSPDIAWKTGTSFGFRDAWAVGTTPDYVIGVWTGNADGEGRPGLTGITA
                     AAPVLFDLINVMGTSGWFPEPSEEMTMIRVCSKSGFRAGPDCPETEEMMACVSGLRSE
                     SCPYHKIIHLDKTGTKQVNADCAPAAEIRNESWFILPPAMEYFYRQKHPDYRPLPPFL
                     PGCTTDRSVAIMEFIYPTSGINIFIPRDQTGELTRIIAEVAHRNPSKKIFWHLDQEYQ
                     GTTRSIHQTEIFAGTGDHVLTVVDEDGNSIMCGFHITGK"
BASE COUNT         1047 a          808 c          830 g          858 t
ORIGIN      
        1 aaagtattat ggctttatcg cgtatagcgc tgccataata atgatcataa tacatcgacg
       61 gaaccggagt attatgatca ttattatggc agcgctatac gcgataaagc cataatactt
      121 tataccctca cacttcttaa caaacaggaa caggctcttc caatactgaa ggaaatttgc
      181 gatcagatga acagttcatc atggttcagc acccagtcgc ttgcctgggg attattcagt
      241 tatatgaaat gggctgaaac attacctgaa ggcaacacca catcatcaaa gataagcatc
      301 accttaaatg gtaaaaagac tgaaaaaacg atcagagcaa aagaactgat gactgaagat
      361 ctggaaatga catctcccga taatccgatt acggttataa ataattcaga agctcctgta
      421 tatgtaacac tggtcagaaa aggaatatca atgcttcctg atgtttcttc tgaggaaaaa
      481 ggcctgagca taaaggtcgg ttatattaat atgaacatga atactataga ccataaaagc
      541 ctgaagatgg gaaccgattt catgatggtt gtcaatgtaa caaacaatac tttctcagag
      601 gtgaacaata ttgctctcac aacaatggtt ccatcgggat gggagataag gaatacccgg
      661 ctttttgaag cccggtacgg aataaagcaa agcgcttacg attacaccga tatcagggac
      721 gacagggtga atacatactt cactctcggc cgggacgaaa caaaaacctt cgtacttgtt
      781 ctgaatgcag cctacaaggg agaatattac cagcctgcaa tctggtgtga agccatgtac
      841 accgaaaatt gttatgcaag gtatccggga acaagggtaa cggtaacagg agaatagatt
      901 tgaaacgaaa attcaggatc ataattgtta ttgccggagt gatgatcact ccggttttaa
      961 tttcgcccat gccacgattc ggggcgccat tatctacagt tgttctggcc ggcgacggga
     1021 gccttctcgg agccaggata gctgatgacg gccagtggcg tttccccggt cctgatgaag
     1081 ttcccgagaa gttcgaaaag gctttgctta catttgagga caggtatttc tactttcatc
     1141 cggggatcaa tcccgtatca atcatcagag cattggtcag gaatataaag gccggagata
     1201 tcgtaagcgg aggcagcaca ataacgatgc aggtcgcccg gctgtcgggc gggaataaat
     1261 cacggacata tgctgcaaaa ctttcagaaa tgctttcagc cttaaagctt gagatcttca
     1321 ggacaaaaaa gggtattctt aaaacctggt caatgaatgc cccgttcgga ggtaatattg
     1381 ttggtcttga ggcagcatcc cgaaggtatc ttgaaaaacc ggcagctgaa attacctggg
     1441 ccgaggcagc gggacttgca gtgcttccaa actctccgtc actggtcttc ccgggaagga
     1501 accagcatat actcagaaaa cgacgcgatg atctcctgaa aaagctttat gaaagaaaat
     1561 attttgattc catcacatat attctggcgc tggatgaacc tcttccgggg gaaccaaaac
     1621 cactcccttc catagctcct catctgactg attatttctt cagcagtaac aacggtgaaa
     1681 tgatccggac tacaattgat gcggctttac aggcaagggc aaatgctata attgaatctc
     1741 acagcaggca gctgtcgggg aatcttatca ataatgccgc atgccttatc gtaaaagtag
     1801 aaacaggcga agttctggca tatgtcggga acagccggac tgataaacca ggcaaccatg
     1861 gtgaagatgt ggacattatc agatccccca ggagcaccgg gagtattctt aaacccatac
     1921 tctacgcggg aatgcagcaa tcaggtgaca tcctccccaa tacccttgtc gcagatatcc
     1981 ccacccgctt cccgggtttc acacctgaga atttcgatca gagcttcagc ggtgcagtgc
     2041 cggcttcgct tgcattatcg cagtcgctta atattccggc tgtaagaatg ctccggaaat
     2101 attctgttga aaaactgctt gacctctttc aaaaaacagg ctttacaact ttcacccggt
     2161 ctgccgatca ttacggatta tcacttatcc tcggcggggg agaagcaaca ctctgggagc
     2221 ttaccggtgt atatgcatca ttatcaaggg ttctgaacag gttcggtaaa gaagggaaat
     2281 attgtcccgg tgactatcac atgcctgttc tcatgacagg ttcaataaac gacaatgaaa
     2341 ggccggttta tggcgagccc gttttatcag catcatcaat atggcttaca tatgaagctc
     2401 ttaaaagagt taacaggccc gaagccgagg ccggatggca atttttctca tcttcccctg
     2461 atattgcctg gaagaccggt accagcttcg gattccgcga tgcctgggcc gtcgggacaa
     2521 ctcccgatta tgttatcggt gtctggacag gtaatgcaga cggagaggga cgaccggggc
     2581 taaccgggat aactgcggct gcaccggtat tgttcgacct catcaatgta atgggaacat
     2641 caggatggtt cccggaacct tctgaagaaa tgacaatgat cagagtatgt tcgaaaagtg
     2701 gcttcagggc aggtcccgac tgtccggaaa cagaagagat gatggcctgt gtaagcggac
     2761 tgagaagcga atcctgccct tatcataaaa taatacatct tgataagacc gggacaaaac
     2821 aggtaaatgc cgattgcgca ccagctgctg agatcaggaa tgaatcatgg ttcatccttc
     2881 cccccgccat ggaatatttt tacagacaaa agcatcctga ttacaggcca ctgcctcctt
     2941 tcttaccagg ttgcactacc gatagaagcg ttgcgataat ggaattcatt tacccgacat
     3001 cagggattaa tatattcatt cctcgcgatc agacaggtga gcttacaagg ataattgctg
     3061 aagtcgcgca ccgcaatcct tcaaagaaga tcttctggca tctcgatcag gagtaccagg
     3121 gaactacccg gtcgatacat cagactgaga tatttgccgg tacaggtgat catgtgctta
     3181 ctgtagtgga tgaggatgga aacagcatca tgtgcgggtt ccatataacc gggaagtaat
     3241 ccgggagaag aggctgtttt cacagatctg atctgccacc caactcccag gtgatggttt
     3301 tacccccaaa acctcccagc gggatttaac ccccaaacct tccagcaggg atctagctcc
     3361 aacccatatc taggccttac ccccaaaccc cccagggggg gcttgaagag tgccgacaca
     3421 gaattttgga atataaaatg ttaagaataa tgaaggtgaa acactcagca gaatttaaag
     3481 tcccccttgg gggatttagg ggtaaaacgg gaaaaggaat gcatccggaa ttatttttaa
     3541 atc
//
LOCUS       DAOOYG010000191         3501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_32104_length_3501_cov_5.516831, whole genome shotgun sequence.
ACCESSION   DAOOYG010000191 DAOOYG010000000
VERSION     DAOOYG010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32104_length_3501_cov_5.516831"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PK288_11260"
     CDS             <1..550
                     /locus_tag="PK288_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPM19548.1"
                     /translation="PVPIVTPTTKAEQGTHDEDILPSEIIRRGLISGEEYQLIENYAQ
                     QVFIRGAEIAADHGLILVDAKYEFGNLDGQVMLIDEVHTPDSARYFYADEYQDRFERG
                     LPQKQLSKEFVREWLMERGFRGEKGQKIPRMTDDFVKQVSERYIELYERITGEKFEPS
                     DITDVRARIEGNCTGFLKSFGL"
     gene            604..2622
                     /locus_tag="PK288_11265"
     CDS             604..2622
                     /locus_tag="PK288_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPM19549.1"
                     /translation="MIVFRRKVLSVLLTATAFLTVIVLSFTAGRANAQVPVERSKDKA
                     VIGGETYYIHIVRKGETAYSISRAYGITVEQLAHENPAAGAILKEGMSLRIPVRIVGT
                     MPVEQPVPYRHVIHDEGKFIYHILGKGETVYYLSKKYQVTEQEIIDANPGLDITRITL
                     GSEIAIPRRIADVVEKAVSVTKPEGYFHRVTSGETLSSIARDYGITLKELRRANRNIR
                     FPKVGDFILIPGMEAVKREVVTEWEPDTVRNAVDSIDIHPGKPEEPVRFRNLSGSFNM
                     AVLLPFFLSENSRRTVADTLKLPKGKKDPRSTTRPEEWIYPASIEFIEMYEGILLAAD
                     TLRSLGLDINIHTYDIKSDTVEITRLIMSDALSGMDLIIGPVYSRNLTIVADYAGKLG
                     IPVVSPVPLFSNSPLDDNPLLFMAGSSLEVAQRAIARKVAEYPDHNIVLIHSTDPEES
                     NDIERFREIISAELHKKDPYELIKIRSLPFYSRSEYGTDSPQRLSRALSVETGNIVVI
                     ASESAPVMSESVMEVRNLSRKFDMKVFGYPEMRELSNIDPKFLFDLGMMVYSPYWIDY
                     SNEDVKNFISRFRKRFFTEPSEKSYAWQGYDIAYYFLGGLALYGREFITHPGMLYTDL
                     LHTDFDFRRKGSNDGFENQKLFLIRYSNTYDLEMIPENNAVSFGQAGE"
     gene            2710..3270
                     /locus_tag="PK288_11270"
     CDS             2710..3270
                     /locus_tag="PK288_11270"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPM19550.1"
                     /translation="MVRESFKDREDSDLVEASVGGDKQAFGEIVNRYQNMVARTVKGM
                     LGDTVLSEDIGQEVFIKLYHSLPEFRGEARLSTYIQKMAVNFSLNEIRRRKRFMSMFA
                     LKENAELREIEFASGDEEKRSDALELVNKALMRLDPKFRIIVVMRLLQGYSTRETAEI
                     LDLPVGTVLSRLSRAQEQLKIIMKKL"
BASE COUNT         1050 a          676 c          871 g          904 t
ORIGIN      
        1 tcctgttccc attgttactc ctaccacgaa agcggagcag ggaacacatg atgaagacat
       61 tctgccttct gaaataataa gaagagggct tataagcggg gaagaatacc agcttattga
      121 aaattatgca cagcaggttt tcatcagggg ggcggaaatt gccgcggacc atggcctgat
      181 tcttgttgat gcaaaatatg aattcgggaa cttggacggg caggttatgc ttattgatga
      241 ggtccataca cctgattccg cccgatattt ttatgcagat gaataccagg accggtttga
      301 aagaggcctt ccccagaaac agctgtcaaa ggaatttgta cgtgaatggc tcatggagag
      361 aggattccgg ggggaaaagg gacaaaagat cccccgtatg acagacgatt ttgttaaaca
      421 ggtttcagaa agatatattg aactttatga aaggatcact ggtgaaaaat ttgaaccctc
      481 tgatataact gatgtcaggg caagaattga aggaaattgc accggatttc tgaaaagctt
      541 cggcctgtga tcattcagaa aagaaaaatg attaataata gtatgacggg aagttttttt
      601 tgcatgatag ttttcagaag aaaggtctta tcagtactgt taaccgccac agcgtttctt
      661 acagtcattg tcctttcatt taccgcaggt cgggcaaacg cacaggttcc ggttgaaaga
      721 tctaaggata aggcagtgat cggcggagag acttattata ttcatattgt ccgaaaggga
      781 gaaacggcat attccatttc cagggcatac ggaattaccg ttgaacagct ggctcatgag
      841 aatccggcgg caggagccat tcttaaggaa ggaatgagct tgaggatccc tgtgaggatc
      901 gtcgggacaa tgccggtgga acaacccgtg ccttaccgcc atgtcattca tgacgaaggg
      961 aagttcatat atcacattct tggtaaagga gaaactgttt attatttgtc aaaaaaatac
     1021 caggtcaccg aacaggaaat tatcgatgcc aatccgggac tggatattac aaggataaca
     1081 ctgggttctg aaatcgcaat tccccggagg attgccgatg ttgttgagaa agctgtatcc
     1141 gtgacaaagc ctgaaggata ctttcacagg gttaccagcg gagaaacact ttcctcaatt
     1201 gcaagggatt acggaataac attgaaggag ctcaggcggg ccaaccgcaa tatccgtttt
     1261 cccaaagttg gagattttat tctgattccc ggaatggagg ctgttaaaag ggaggtagtt
     1321 accgagtggg aaccggatac tgtcaggaat gcagttgata gtattgatat tcatcccggg
     1381 aaaccggaag aacctgtccg gttccggaac ctcagcggat ccttcaacat ggctgtgctg
     1441 ctgccatttt tcctcagtga aaactccaga agaacagtgg cggatacatt aaaacttccg
     1501 aaaggaaaga aggatccaag aagcactacc aggcctgaag agtggattta tcccgccagt
     1561 attgaattca ttgaaatgta tgaagggatc cttctggcag cggatacttt gaggtcactt
     1621 gggcttgata tcaacattca cacttatgat atcaaaagcg atactgttga aataacccgg
     1681 ctgatcatgt ccgatgcact ctcaggaatg gatctcatta tagggccggt atattccagg
     1741 aacctgacca tagtggcaga ttatgccgga aaactgggta ttcctgtggt ctcgccggta
     1801 cccttgttca gcaattcacc tctcgacgac aatccgctgc tttttatggc agggtcatca
     1861 cttgaggtgg cccagagggc cattgcacga aaggttgcag aatatccgga ccataatatt
     1921 gtgctcattc attctacaga tccggaagaa agcaatgata ttgaacggtt cagggagatt
     1981 atttcagcgg aattgcataa aaaagatccg tatgaattga taaagatcag gagtctgccc
     2041 ttttacagca ggtcagaata cggcaccgac tcgccgcaga gattgtcgag ggctttatct
     2101 gttgaaactg gaaatattgt tgtaatagct tcggaaagcg ccccggtaat gagcgagtca
     2161 gtcatggaag tcagaaacct gtcgagaaaa ttcgacatga aagtattcgg ataccctgaa
     2221 atgagggaac taagcaatat tgaccccaaa tttctctttg accttggaat gatggtatat
     2281 tcaccatatt ggattgatta ctccaatgag gatgtgaaaa acttcatatc ccggttcagg
     2341 aagagatttt tcacggagcc ttccgagaaa agctatgcct ggcagggata tgacattgcc
     2401 tactatttcc tgggtggcct ggctttgtac ggcagggagt tcataaccca tccggggatg
     2461 ctttataccg atctgcttca taccgatttc gacttcagga gaaaaggcag caatgatggc
     2521 tttgaaaatc aaaaactctt ccttataaga tattccaaca cttatgatct tgaaatgata
     2581 ccggaaaaca atgcagtaag cttcggacag gctggtgagt agacgactgc tgattttggt
     2641 acctgttttt ttgttaataa gaattaacaa agcggaataa ttaaatggtt tcatttgtct
     2701 aaatgtcaaa tggtcagaga atccttcaaa gacagggaag acagtgatct tgttgaagcg
     2761 tctgttggag gcgataaaca ggcctttggt gaaattgtga accgctatca gaacatggtg
     2821 gccagaacgg tgaaggggat gctcggtgat actgttttat cggaagatat aggacaggaa
     2881 gtattcatta aactctatca ctcacttccg gagttcaggg gtgaggccag gctttcaacc
     2941 tatattcaga agatggcagt caattttagc cttaatgaga tacgcagaag aaaaaggttt
     3001 atgtcgatgt ttgcactgaa agaaaatgcg gaattgcggg agattgagtt tgcaagcggg
     3061 gatgaagaga aaagatcgga tgcactcgaa cttgttaaca aggcactcat gaggctcgat
     3121 ccgaaattca ggatcattgt tgtcatgaga ttacttcagg gatattccac aagggaaacc
     3181 gccgagattc ttgacctgcc tgtcggaacc gtgctttcac ggctttcaag ggcacaggaa
     3241 caactgaaaa tcattatgaa gaaattgtag ttcatggagc ctttatattg cataattaaa
     3301 acctggcgtg caactcatgc gttgcaaaac ggtcagcgga aacagtttga gattgtgtct
     3361 gcctgccgta tgaggcagtg catgctttaa aagattaata gcttatgaaa actgatgaat
     3421 taaagggact catattaaag gcgcttgaaa cagaacaaac gcctgacagt tttatgcagg
     3481 aactggaaga agccggcgtt a
//
LOCUS       DAOOYG010000192         3329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_34416_length_3329_cov_6.438302, whole genome shotgun sequence.
ACCESSION   DAOOYG010000192 DAOOYG010000000
VERSION     DAOOYG010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34416_length_3329_cov_6.438302"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1486
                     /gene="gdhA"
                     /locus_tag="PK288_11275"
     CDS             146..1486
                     /gene="gdhA"
                     /locus_tag="PK288_11275"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPM19551.1"
                     /translation="MDPRVKQFMTVIKAKDPGESEFHQAVQEVAESLIPFIEENPKYR
                     HAKILERIAEPERVIIFRVPWLDDKNEIQINRGFRIEMNSAIGPYKGGLRFHPTVNLA
                     ILKFLAFEQTFKNSLTTLPMGGAKGGSDFDPKGKSDNEVMKFCQSFMTELFRHIGPDT
                     DIPAGDIGVGGREIGFLFGQYKRLRNEFTGVLTGKGIEYGGSLMRPEATGYGATYFAQ
                     EMLATRKETMKGKTVCISGSGNVAQYAAEKISELGGKVITMSDSNGFVYDPHGINGER
                     LEYLKNLKNVQRGRIKEYADKYGVEYFEDQRPWIIKCDIAMPNATQNEINGDEARTLV
                     RNGCICVTEGANMPSTLEAVEVFLNAGILYGPGKAANAGGVATSGLEMSQNSMRLPWT
                     RKEVDDRLLLIMKEIHATCVKYGKKPDGFINYVDGANIGGFVKIANAMLAQGIV"
     gene            1486..2253
                     /locus_tag="PK288_11280"
     CDS             1486..2253
                     /locus_tag="PK288_11280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPM19552.1"
                     /translation="MHLIDTHTHIYLPEFDNDRDQVIARSLESGVEKLLMPNIDQESF
                     PRMMAAVNRYPGVCLPMNGLHPTSVKEDFQFQLEKIECLAAEYTFCAIGEIGIDLYWD
                     RTYLEEQIFVFKKQVELALAKNMPVVIHSRESFAELLTALEEFRGSGLKGVFHAFTGN
                     REHAEKAVELGFKLGIGGIVTFKNSGLDSVVKEVGIENIVLETDSPYLAPSPFRGKRN
                     ESSYLCIINKKVADICEMNEEDVASVTYSTSAELFGL"
     gene            2262..3299
                     /locus_tag="PK288_11285"
     CDS             2262..3299
                     /locus_tag="PK288_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010799836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPM19553.1"
                     /translation="MKPRAGTSILIIYTGGTIGMVHDPVTGSLVPINFSHITDHVPEL
                     QKSGFSIKSVSFDPVRDSSNIDPAIWVKMAETIEENYHKYDGFVILHGTDTMAYSASA
                     LSFMLGNLQKPVIFTGSQLPIGLLRTDGRENLVTAIEFASEQENGKPLVPEVCIYFDS
                     ELTRGNRTTKFSAEHFDAFNSPNYPPLAVSGLHLKFNRDFIRYGDADKKLIVRKKFDT
                     NVAILKIFPGMGRSLVQSIINTPGLKGLIIETFGSGNAPTYGWFIEDLRNFIMKGGII
                     FNVTQCHGGSVEMGLYETSRDLLAAGIVSGKDITSEASVTKLMFLLGNCSSRQEVTDC
                     LGRSIAGTSVL"
BASE COUNT          989 a          687 c          821 g          832 t
ORIGIN      
        1 agagaactta tattatacgg cactatggcc gcttaaaact ttaacgaact attggcctta
       61 ataaattaat attttttgcc ttcagttcag cttcaattat catttttaat aattttagcc
      121 gattattcaa ataaataacg ttaacatgga tcccagggta aaacagttca tgacggtgat
      181 caaagccaaa gatcctggtg aaagtgaatt tcatcaggca gtacaggaag tggcagaatc
      241 acttattccc tttattgaag aaaacccgaa atacaggcac gcgaaaattc tagagagaat
      301 tgctgaacct gagagagtga ttatttttcg tgttccatgg ctggatgata aaaatgaaat
      361 ccagataaac cgggggttca ggatagaaat gaacagtgcg atcggaccct ataagggagg
      421 actgagattt catcccacgg tcaatttggc gatcctgaaa ttccttgcat ttgaacaaac
      481 ctttaaaaac agcctgacca ccctcccgat gggcggggcc aaaggtggtt cggactttga
      541 cccgaaaggg aaatccgaca atgaagtaat gaaattctgt cagagtttca tgactgaact
      601 gttcaggcac attggacctg acactgatat tccggcaggc gacataggcg tgggaggaag
      661 ggaaatcggt tttttgttcg gacaatacaa gcggctgcgg aatgaattca cgggagtgct
      721 taccgggaaa ggaattgaat atggaggttc gctcatgcgg cccgaggcaa caggttatgg
      781 cgctacatat tttgcccagg aaatgctggc caccaggaag gaaaccatga agggcaagac
      841 agtctgcatc tcaggttcgg gcaatgtggc gcaatatgct gcagaaaaga tctcagaact
      901 gggcgggaaa gtgataacca tgtccgattc gaacggattt gtttacgacc cgcatggcat
      961 taacggcgaa aggcttgaat acctgaagaa tctcaaaaat gtacagaggg gaagaataaa
     1021 agagtatgct gacaaatacg gtgttgaata ttttgaagat cagcggccat ggataattaa
     1081 atgcgatatt gccatgccga atgccaccca gaatgagatt aacggagatg aagcccgcac
     1141 actggtaagg aacggatgca tatgcgtgac tgaaggtgca aatatgccat caaccctcga
     1201 agccgttgaa gttttcctga atgcaggcat cctgtacgga cccggcaaag ctgcaaatgc
     1261 aggaggggtt gctacctccg gactggagat gagtcagaat tccatgaggc tcccgtggac
     1321 caggaaagaa gtcgatgaca ggttgttgtt aatcatgaag gaaattcatg caacctgcgt
     1381 caaatacggt aaaaaacccg atggctttat aaactatgtt gacggtgcca acataggagg
     1441 ctttgtgaaa atagccaatg ccatgctggc acaaggcatt gtgtgatgca ccttattgat
     1501 acacatacac atatctatct gccggagttt gacaatgaca gggaccaggt gattgcaagg
     1561 tcactggaaa gcggtgtcga aaaactgctt atgccaaaca ttgatcagga gtcatttccc
     1621 cgtatgatgg ctgcagtaaa ccgctatccg ggggtatgcc tgcccatgaa cggacttcat
     1681 cccacttcag ttaaggaaga ttttcagttt cagttggaaa aaatagaatg tcttgcagcg
     1741 gaatacacct tttgcgcaat cggtgagata ggcattgatc tttattggga cagaacttac
     1801 ctggaagagc agatttttgt ttttaaaaag caggtggaac ttgcccttgc gaaaaatatg
     1861 ccagttgtca ttcattccag ggaatcattc gctgaattac tcactgctct tgaagaattc
     1921 aggggatctg gactgaaagg cgtgtttcat gcttttaccg ggaaccggga acatgccgaa
     1981 aaagctgtag aattgggttt taaactgggg ataggaggca ttgtcacctt taaaaactcg
     2041 gggcttgaca gtgtagtgaa ggaagtcgga atagaaaata tcgtactcga aaccgattca
     2101 ccctatctgg caccctcccc ttttcgtgga aaacgcaatg agagttcata tctttgcatt
     2161 ataaacaaaa aagttgcgga tatttgtgag atgaatgaag aagatgttgc ttccgtaact
     2221 tactcaacat cagccgaact tttcggattg taatgcagat tatgaagccc agggcaggaa
     2281 cctccattct tattatctac actggaggga ccattggaat ggtccatgat ccggtaacag
     2341 gatcgctggt cccgataaat ttcagccata ttaccgatca tgtgccggag ttgcaaaagt
     2401 caggcttcag cataaagtca gtatcattcg atcctgtaag ggattcatca aacattgatc
     2461 cggccatatg ggtgaaaatg gcagaaacaa tcgaggaaaa ttaccataaa tatgatggtt
     2521 tcgtgatcct gcacggaacc gacaccatgg cctattcagc atcggcactg agcttcatgc
     2581 tcgggaatct ccagaaacct gttattttta ccggatccca gcttcccatc ggacttcttc
     2641 gtaccgatgg cagggagaat ttggttacag ccattgaatt tgcttcggaa caggaaaacg
     2701 gaaaaccgct agtacctgaa gtatgtattt attttgacag tgagctgacc cgtggtaatc
     2761 ggacgacgaa attcagtgcc gagcactttg atgcattcaa ttcgcccaat tatccgccac
     2821 ttgccgtttc ggggctccat ctcaaattca atcgcgattt catacgctat ggcgatgctg
     2881 acaaaaaact aattgtcagg aaaaaattcg acaccaacgt tgcgatactg aaaatattcc
     2941 cgggaatggg ccgaagcctt gttcaatcga tcataaacac gcccggctta aaaggtctta
     3001 taattgagac atttggttca ggcaatgccc cgacctatgg ctggtttata gaagatctga
     3061 gaaatttcat catgaaaggg ggaataatct tcaatgtaac ccagtgtcac gggggaagtg
     3121 tagaaatggg tctgtacgaa accagcaggg atctgttggc tgccggtata gtaagcggga
     3181 aggacataac ctcggaagcg tccgttacaa aactgatgtt cctgctcggg aactgttcgt
     3241 caaggcagga ggtgaccgat tgcctgggca ggtcaatagc cggaacatca gttttgtaac
     3301 ggacgcttcc gaggttatgt ccttcccgc
//
LOCUS       DAOOYG010000193         3213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_36206_length_3213_cov_8.563965, whole genome shotgun sequence.
ACCESSION   DAOOYG010000193 DAOOYG010000000
VERSION     DAOOYG010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36206_length_3213_cov_8.563965"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PK288_11290"
     CDS             complement(<1..521)
                     /locus_tag="PK288_11290"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM19554.1"
                     /translation="MKMKKYYILNLIPVVFLSALIMSACTDLDIDVESELTPENFPTQ
                     PSHFEAAKGPIYSNLPSAYAIDYWRLQSLSTDEVLIPARGGNWDDGGIYRELHKHTWT
                     KDHSIVRGIWEYGFRGISSCNQILTMFDAVPEGELRNKQFAEIRMMRAFYYFLMLDNF
                     GNVPILEKFGDDTP"
     gene            complement(555..>3213)
                     /locus_tag="PK288_11295"
     CDS             complement(555..>3213)
                     /locus_tag="PK288_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPM19555.1"
                     /translation="VMAEESYLVDEVVVVGYGRTAKKDLTGAVTVVEAEEMNKGVYSS
                     PTQMLQGKVPGLNITRSGDPSGSASITLRGPSTLRTGAAMEPFYVIDGVPGASIDAVA
                     PDDIVSINIMRDASSTAIYGSRAANGIILITTRRAKTNESYFTYNGYAAAETVSNRIE
                     MLSAEELRTFLTANSKTPLDEDGSSTNWQDEVMRTGISHNHNFSFGNSSENTRYSASI
                     NYLNNQGIIKTSSMDRLTARISVEQQAVNDRVTLGLNLSNTETNQHVIRQELFANMLK
                     YLPTIGIYNPDGTYYQNTQNSNYHNPVAILDNDLDDRKISNLLANMTLKVNILKGLTY
                     DASASYQNTDIKRNVYDKQASTLKIGSNGFALRSEYANRKTLFENYLTYENLFGKHSV
                     KILAGYSWQQDKLNDGFQAANANFVTDDLLYNNLSLGSSPDKVTTQERFGTATIQTLR
                     IISAYSRLNYQFADRYLLQATIRRDGSSAFGENNRWGTFPAVSVGWRITNEPFMQNQK
                     LFSDLKLRIGYGISGNSLGFDPMISKLKYGLTGVTYINGEQVQSIGVTQNENANLKWE
                     STAMSNIGLDFAFLKGRITGTLEVYDKETKDLIWYYPVKVPPYLYPYLWANVGEISNK
                     GIEFQIDATPVRTNNFQWTTSFNISSNKNEVKSLSNDQFETPEIGIRTAAIGGKGQSG
                     NPTQVIMEGQPLGTFFLRQWAGRDESGVSLFFAANGDKLRVVTVDDFAICGSAQPKFL
                     FGWNNSFAYRKFDLSFFFRGVAGNKILNGTLAGLNDVNYAASNNIPKWSLREPVGDIN
                     SFYYSDRFLESGTYARLDNATLGYTIDLSKLRIKSARIYVTGNNVFVLTGYRGVDPEI
                     NMGGIEPGIDNNNFYPKTRSFILGLNLNF"
BASE COUNT          826 a          701 c          708 g          978 t
ORIGIN      
        1 ggagtatcat ctccgaattt ttcaaggatc ggcacgtttc cgaaattatc aagcataagg
       61 aaatagtaaa aagctctcat cattcttatt tcggcaaatt gtttgttcct caattcccct
      121 tcagggactg catcgaacat cgtcaggatc tggttacagg agcttattcc cctgaatccg
      181 tattcccata tccccctgac aattgaatga tcttttgtcc aggtgtgttt gtgaagttcc
      241 ctgtaaattc cgccatcatc ccagttacct ccgcgggcgg ggataagtac ctcatcggtg
      301 gacaatgact gaagtctcca gtaatctatt gcataagcgc tcggtaaatt ggagtaaata
      361 ggacccttag ccgcttcaaa atgagatggc tgggtaggaa agttctctgg tgtgagttct
      421 gattcaacgt ctatatcaag atcggtgcag gcgctcatta tcaatgctga caaaaagacc
      481 accggaatca gattcagtat ataatatttc ttcattttca tattttctga ttttaattcg
      541 tttcgtgacc ggatttaaaa attaagattt aaacccagaa tgaatgaacg tgtttttggg
      601 tagaaattat tattgtcaat accgggttcg atccctccca tattgatttc aggatcaaca
      661 cccctgtacc cggtcagaac gaaaacattg ttcccggtaa catagatacg ggcgcttttg
      721 atcctgagtt ttgaaagatc gattgtatat ccgagtgttg cattgtcaag ccttgcatat
      781 gtcccgcttt caaggaagcg atcggagtag tagaatgagt ttatatctcc gacaggttcg
      841 cgcagtgacc acttcggaat attgttgctt gctgcatagt taacatcatt gagtccggca
      901 agggtaccgt taagtatctt atttcctgca acgcctctga agaaaaagct cagatcaaat
      961 ttccggtatg cgaaactgtt attccatccg aacaggaatt taggttgcgc tgaaccgcag
     1021 atagcgaaat catcgactgt tacaacacgt aatttatcgc cattggctgc aaagaacaat
     1081 gatacgccac tttcatccct tcctgcccac tgcctcagga agaaagtacc cagaggttgt
     1141 ccttccatga tgacctgtgt aggattgcct gactgacctt taccaccgat tgcagctgtt
     1201 ctgataccaa tttcgggagt ttcaaactga tcatttgaaa gggatttcac ttcgttttta
     1261 ttggacgaaa tattgaaaga tgttgtccat tggaaattat ttgtccttac gggtgttgcg
     1321 tcaatctgga attctatccc tttgttgctt atttcgccga catttgccca aaggtaagga
     1381 taaaggtaag gaggtacttt taccgggtaa taccagatca gatccttggt ctccttgtca
     1441 taaacctcca gggtaccggt aattctgcct ttcaggaaag caaagtcaag accgatattc
     1501 gacattgccg ttgattccca tttaaggttg gcattctcgt tctgggtaac cccgattgac
     1561 tggacctgct caccattaat ataagtgacg cctgtaagtc cgtatttcag tttcgagatc
     1621 atgggatcga agccgaggga attcccgctt ataccatagc cgatacggag cttgagatca
     1681 ctgaacagtt tctgattctg cataaaaggt tcattggtga tcctccatcc aacagagacg
     1741 gcagggaatg tcccccagcg gttattctca ccgaaagccg aggatccgtc acgccttatt
     1801 gttgcctgca gaagataacg gtcggcaaac tgataattga gccgcgaata agcagaaatg
     1861 atccttaatg tctgtatcgt ggcagttccg aaacgttcct gagtggttac tttgtcagga
     1921 gaactgccaa gtgaaaggtt gttataaaga agatcgtctg taacaaaatt ggcattggcc
     1981 gcctggaacc cgtcgttaag cttgtcctgc tgccaggagt aacctgcaag tatcttgaca
     2041 gagtgctttc caaacaggtt ttcataagta agataatttt cgaacagtgt tttacggttt
     2101 gcatattcag agcgaagggc aaaaccattc gatcctattt taagggtgga agcctgttta
     2161 tcatatacat tccgcttaat gtccgtattc tggtatgaag cgctggcatc ataggtaagt
     2221 cctttgagaa tattgacctt taatgtcatg tttgcaagaa ggttgctgat cttcctgtca
     2281 tcgaggtcat tatcaaggat agccacggga ttatggtagt tgctgttttg tgtgttctga
     2341 taatatgtgc catcgggatt gtaaattcct attgtcggca aatatttcag catatttgca
     2401 aaaagttcct gccggattac atgctggttt gtttcagtat ttgaaagatt cagtcccagt
     2461 gtcactctgt cgttaacagc ctgctgttcc acactgatcc ttgcagtcag gcggtccatt
     2521 gaacttgttt ttataatccc ctggttattg aggtagttta tgcttgcact gtatcttgtg
     2581 ttttcggatg aattgccgaa cgagaagtta tgattatggg aaatccctgt tctcattacc
     2641 tcatcctgcc agttggtgct ggatccgtct tcgtcgagcg gtgttttact atttgccgta
     2701 aggaaagtcc ttagttcctc tgccgaaagc atttctatcc ggtttgaaac tgtttctgca
     2761 gcagcgtaac cgttataagt gaaataggat tcattggttt tggccctgcg tgtggttata
     2821 agaatgatcc cgtttgcggc ccgtgaacca taaatagctg ttgaagatgc atccctcatt
     2881 atatttatgc ttacaatatc atcaggggcc acggcatcaa ttgatgcacc gggaacacca
     2941 tcaataacat aaaaaggctc catggcagcg cctgttctca gtgttgatgg tccgcgaagg
     3001 gttattgatg cactacccga cggatcaccg ctccttgtaa tattgagtcc gggtacttta
     3061 ccctgaagca tctgggtggg ggatgaatag actcccttgt tcatttcttc agcttcaaca
     3121 acagttacag ctccggtgag atctttttta gcagtccgtc catatccgac aacaaccact
     3181 tcatcgacca ggtatgattc ttcagccata aca
//
LOCUS       DAOOYG010000194         3151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_37264_length_3151_cov_5.935401, whole genome shotgun sequence.
ACCESSION   DAOOYG010000194 DAOOYG010000000
VERSION     DAOOYG010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37264_length_3151_cov_5.935401"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /gene="rpmD"
                     /locus_tag="PK288_11300"
     CDS             complement(<1..167)
                     /gene="rpmD"
                     /locus_tag="PK288_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPM19556.1"
                     /translation="MARIRITQIRSKIGRPERQKRTLEALGIRKLNQSVEHEATAQIL
                     GMVERIRHLVKV"
     gene            complement(182..697)
                     /gene="rpsE"
                     /locus_tag="PK288_11305"
     CDS             complement(182..697)
                     /gene="rpsE"
                     /locus_tag="PK288_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPM19557.1"
                     /translation="MGNGIRKVKTSDLELKDRLVNIQRVTKVTKGGRTFSFSAIVVVG
                     NEDGIVGHGLGKASEVTTAIAKGIEDAKKNLIKVPVIHGTVPHEQVAKFGGAKVFIKP
                     ATEGTGVKAGGAMRAVLESVGVRNVLAKSKGSSNPHNLVKATFAALLEMRDAFAIAEQ
                     RGISMERVFNG"
     gene            complement(704..1063)
                     /gene="rplR"
                     /locus_tag="PK288_11310"
     CDS             complement(704..1063)
                     /gene="rplR"
                     /locus_tag="PK288_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPM19558.1"
                     /translation="MALTKLERRSRIRMRIRKKISGTAEKPRLAVFRSNKQIYVQVVD
                     DQKGVTLLSASSNEKGFAGKTGMKKTEQAKLVGKMLAERCLEKGISTVVFDRSGYKYH
                     GRIKSLADTAREGGLKF"
     gene            complement(1096..1650)
                     /gene="rplF"
                     /locus_tag="PK288_11315"
     CDS             complement(1096..1650)
                     /gene="rplF"
                     /locus_tag="PK288_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPM19559.1"
                     /translation="MSRIGKLPVNLPHGVTVNISDTNVVSVKGPLGELTQKIDGDMKV
                     EVVDNTIVITRPTDSKNHKSLHGLYRALIANMVTGVSQGFKKELELVGVGYRAEAKGQ
                     LLEMSLGFSHDVIMQLPEEVKVETKTERRSNPIITLTCADKQLIGHVAAKIRSLRPPE
                     PYKGKGIKFVGEQLRRKAGKSASV"
     gene            complement(1667..2065)
                     /gene="rpsH"
                     /locus_tag="PK288_11320"
     CDS             complement(1667..2065)
                     /gene="rpsH"
                     /locus_tag="PK288_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPM19560.1"
                     /translation="MTDPIADLLTRIRNAIHAGHRVVEAPASNLKKEIARILFEKGYI
                     LSYKVVEGDTPQGILKIALKYNPRSRKPAIKKIQRASRPGLRYYAGVDEIPRVLNGLG
                     IAIISTSRGLMTDKEAKKENIGGEVLCYVY"
     gene            complement(2103..2372)
                     /gene="rpsN"
                     /locus_tag="PK288_11325"
     CDS             complement(2103..2372)
                     /gene="rpsN"
                     /locus_tag="PK288_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HPM19561.1"
                     /translation="MAKESMKAREVKRARLTEKYAAKRARLKAEGDYVGLSRLPRNSN
                     PNRLRNRCKITGRTRGYMRQFGVSRITFREMASFGLIPGVKKASW"
     gene            complement(2377..2928)
                     /gene="rplE"
                     /locus_tag="PK288_11330"
     CDS             complement(2377..2928)
                     /gene="rplE"
                     /locus_tag="PK288_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPM19562.1"
                     /translation="MYTPALKTRYKEEIIPALMKEFGYKTVMQVPRLEKIVLNQGVGK
                     AIADKKLLEFSVKELTDIAGQKAVQTYSTKDISNFKLRRNMPIGVMLTLRKNRMYEFL
                     ERLISVALPRIRDFKGISSKLDGRGNYTLGITEQIIFPEIDLDKIAKIMGLQVTFVTS
                     AKTDEEALALLKQFGLPFKNIKN"
     gene            complement(2928..>3151)
                     /gene="rplX"
                     /locus_tag="PK288_11335"
     CDS             complement(2928..>3151)
                     /gene="rplX"
                     /locus_tag="PK288_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPM19563.1"
                     /translation="EKAIVEGLNLVSKHTKPNAKSPQGGIIRKEAPIHISNLMLIDPT
                     SGKPTRIGRRLNSNNKLVRYSKKSGEEIK"
BASE COUNT          696 a          790 c          611 g         1054 t
ORIGIN      
        1 accttaacca ggtgacgtat cctttcaacc attcccagga tctgagcggt tgcttcgtgt
       61 tcaacactct ggttcaactt gcggattccg agtgcttcaa gggttctttt ctgcctttca
      121 ggtcgtccga tcttgcttct tatctgggta atgcgaattc ttgccatttc tcaatgcttt
      181 tctatccgtt aaacactctt tccattgaaa ttcccctctg ctcagcgatt gcgaatgcat
      241 cccgcatctc cagaagagcc gcaaaagtgg ctttcacaag gttatgagga tttgatgaac
      301 cctttgattt ggccagcacg tttctcactc ccacgctttc aaggactgca cgcattgcgc
      361 ctcctgcctt cactcccgta ccttcggttg caggcttgat aaagactttt gccccgccga
      421 attttgccac ctgctcatgg ggaacggtac catgtatgac aggtaccttt ataaggttct
      481 tctttgcatc ctcgatccct tttgcaatgg cagtagtaac ttcactggcc ttccctagtc
      541 cgtgaccgac aatcccatct tcatttccca cgactacgat agctgaaaaa ctgaatgtac
      601 ggcccccctt ggtaactttt gtaaccctct ggatatttac gagcctgtct tttagttcga
      661 gatcgctggt tttaactttt cttattccgt ttcccataat tgattagaat tttaagccgc
      721 cttcgcgggc tgtatcagcc aatgatttta ttctgccatg atatttgtaa ccgcttctgt
      781 cgaagactac ggtggatatc cctttttcaa ggcatctttc ggcaagcatc tttccaacca
      841 gctttgcctg ttctgttttt ttcataccgg tttttccggc aaagcctttc tcattcgaac
      901 ttgctgaaag aagggttacc cctttctgat catcaaccac ctgaacatat atctgcttat
      961 tactgcgaaa aaccgcaagc cggggcttct ctgccgttcc gcttatcttt ttcctgatcc
     1021 tcattcttat cctggatctt ctttccaact tggttaaggc cataatttta aattttttcc
     1081 ggtttacttc cctactcaga cgctcgcaga ttttccagcc tttcttctaa gctgttctcc
     1141 gacaaattta atccccttgc ccttgtatgg ttcaggcgga cgaagggacc tgatttttgc
     1201 tgccacatga ccaataagct gtttatcagc acaggtcagg gtgattatcg ggttgcttct
     1261 cctctcagtt ttggtttcaa cctttacttc ctcaggcagc tgcataatta catcatgtga
     1321 aaatcccagg ctcatctcaa gtaattgtcc tttcgcttca gccctatatc cgacacccac
     1381 cagttcgagc tcttttttga acccctgcga tactccggtc accatattgg caatgagagc
     1441 cctgtagagt ccatgcagcg acttgtgatt tttcgaatct gtaggacggg ttataacaat
     1501 agtgttatcc acaacttcca ccttcatgtc gccgtcgatt ttttgggtaa gttcacccaa
     1561 aggtcctttc acactaacca cattggtgtc gctgatattt accgtgacac catggggcag
     1621 gtttacaggt aattttccta ttcgtgacat ttatttatcc tcctgattaa tatacataac
     1681 atagtacttc cccgccaata ttttcctttt tggcctcctt gtcagtcatc agtcccctgg
     1741 aagttgaaat gatagctatt ccgagtccgt tcagaaccct gggaatttca tcaacaccag
     1801 cataataccg gagcccgggg cggcttgccc gctggatctt cttgatggca ggctttctgc
     1861 tcctcggatt gtatttcaat gcaatcttaa ggataccctg gggagtatct ccctctacaa
     1921 ctttatagct cagaatataa cccttctcga acaatattct ggcaatttcc ttcttaagat
     1981 ttgatgccgg tgcttcaaca accctatgac ctgcatggat ggcatttctt attcgggtca
     2041 gcaaatctgc aattggatca gtcatcttat atttaatata tggttaatat ttgctttaat
     2101 gtttaccagc tggctttttt cacgccagga ataagaccga aagatgccat ttccctgaaa
     2161 gttatcctgc tgacaccaaa ctgccgcatg tacccccttg ttcttccggt gatcttgcac
     2221 cggttacgca gcctgttggg atttgaatta cgcggcaaac ggcttaatcc gacataatca
     2281 ccttcggctt tgagtctggc cctttttgca gcgtattttt cagtcagtcg tgcacgttta
     2341 acttcacggg ccttcattga ttccttagcc atataatcag tttttaatgt ttttaaatgg
     2401 taatccaaat tgtttgagaa gagccaatgc ttcctcatca gtttttgcag aagtaacgaa
     2461 tgtgacctga aggcccataa tttttgcgat tttgtcaaga tctatttccg ggaaaataat
     2521 ctgttcggtt attccaagcg tgtagttgcc gcgtccgtca agctttgagc tgattccctt
     2581 gaaatcgcgg attcttggaa gtgccacgga aataagcctt tccaggaatt cgtacatcct
     2641 gtttttgcgg agggtgagca taacaccgat aggcatgttg cgtctcagtt tgaagttgga
     2701 aatatccttt gttgaataag tctgaacggc cttctgcccg gcaatgtctg ttaactcctt
     2761 gacactgaat tcaagcagct tcttatcggc tattgcctta ccgactccct gattaagtac
     2821 aattttttcg agtctgggaa cctgcataac ggttttataa ccgaattcct tcatcagtgc
     2881 aggaatgatc tcttctttgt atctggtttt caatgcaggt gtatacatta ctttatctcc
     2941 tctcctgatt tttttgaata tctcactagt ttattgttgc tgttgagcct tcttcctatc
     3001 cttgttggtt tgcctgaagt agggtcaata agcataaggt ttgaaatatg aatcggggcc
     3061 tctttccgga taatgccgcc ctggggcgat tttgcattag gctttgtatg cttggaaacc
     3121 aggttaagtc cttcaactat agctttctcc t
//
LOCUS       DAOOYG010000195         3114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_37905_length_3114_cov_5.311540, whole genome shotgun sequence.
ACCESSION   DAOOYG010000195 DAOOYG010000000
VERSION     DAOOYG010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37905_length_3114_cov_5.311540"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1381)
                     /locus_tag="PK288_11340"
     CDS             complement(266..1381)
                     /locus_tag="PK288_11340"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPM19564.1"
                     /translation="MTEFTGEPGCYGIVQYTNDPDIKFTIQEGSLSIYRELPIASAYD
                     FIPGQGNTQYTEETYDFSYKKAFRSLSGKVSKERIRNSVYYLSDDPLPRRVLNYTLPG
                     HSLSTLAEADIWIEGRLTGAGYSPWQDETKVRAFGRDLSKPLAHQYAAPPADAPEYPA
                     HNIIAELTGSADRENIIVVIAHKDSQSWIASPGANDNAVGTSAALELADVLKDYKPLH
                     TIRFIFCNEEHTPWTSITAVNGIRSEGKDVLAAINLDGIGGKSAEQAGKLINITRYTT
                     PEGERLADLNSMLNKRFSIGLEQSKFRSDSPGDDDGSFINGGFPWAVLNIGSMPYADP
                     NYHIETDTADKVDYDTTERVVKLTLALILHLDRNGRP"
     gene            complement(1812..>3114)
                     /locus_tag="PK288_11345"
     CDS             complement(1812..>3114)
                     /locus_tag="PK288_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19565.1"
                     /translation="YASLMCEDPFHPVMAEGEKPNKRLDPIFWQSVPTPANLLFPDTG
                     AEGAMSESSRSYGAAYQRYPNRIALFVAENTSCASYCVHCQRAKSLDSTVAVSTMEIN
                     KGLFYIGYNKNINEVLVTGGDALMISKSRLRYVLEELSRIPHLRVIRIATRVPVVLPM
                     AVTDELLDLINESANRYTSGLRKYVYFMTHINHYHEITESLAAVSRKIHGHGFTIRNQ
                     TVLLNHVNDYYETLAETFRRMFWIGIHPYYLLQCHKEKGIVHFITPVQIGKIYIKHLQ
                     GWISGITMPRYAANIEGGGGKVLLMPSGHDTLGEEIHIDEKISESFALVSTWDGKKIY
                     RYESLGRATRQEYEEAVSLMDKFIGRKGIFLPKVIIVDEKGNHIETTNRSKLPVFERF
                     KKSQLLGYEIHEKDMPLTNPQDILPELEKQFARSKYVNNGK"
BASE COUNT          836 a          705 c          665 g          908 t
ORIGIN      
        1 gagatatccc ctgaaaagca gatcggaacc acgatagtga attcattcag ggatatttct
       61 gttcacaata tgcctgagta ttattatcat gttgttgcag ttgatcagtc gggaaataca
      121 agcaagtaag aaactgcccg taagtgactg atgccagaaa atcaatcccg gcaatctttg
      181 atccggcttc aggctgtttt atatgtacct cgacgtgcca tgtatacctt tccgggtaat
      241 gaaaccaatg gcgtatacct gattattatg gtctcccgtt acgatcgaga tgaagaatca
      301 gtgcaagggt aagtttaaca acacgttccg tagtatcata atcaacttta tcagccgtat
      361 ctgtttcaat atgataattc gggtcagcat agggcattga accgatattg agaactgccc
      421 agggaaatcc gccgttaata aaggaaccat catcatcccc gggtgagtcg cttcggaatt
      481 ttgactgctc caggccgatg gaaaatcgct tgttaagcat tgaattcaga tcagcaagcc
      541 gttcaccttc gggagtggta tatcgggtga tgttgatcag ttttcctgcc tgttcagcgg
      601 atttgccgcc gataccatca aggtttatgg cagccagtac atcctttcct tcactcctga
      661 taccgttgac agccgtaatg ctggtccagg gagtgtgctc ttcattgcag aaaataaagc
      721 ggatggtatg aaggggcttg taatctttca gtacatctgc caactcaaga gcagctgatg
      781 taccgaccgc attgtcgttt gcccctggcg aagcgatcca gctctgggag tctttgtggg
      841 caatcaccac tatgatattc tcccggtcag ctgaccccgt taactctgca atgatattat
      901 gggcaggata ttccggtgca tcagcaggcg gcgcagcgta ttgatgtgcc agaggttttg
      961 aaagatccct gccaaaggca cgaacctttg tttcatcctg ccagggactg taaccggctc
     1021 ctgtaagcct gccctcaatc cagatatctg cttcagcaag ggttgacagg gaatgccccg
     1081 gcaatgtgta attcagaacc cgtcttggca aaggatcatc tgataaataa tagacactgt
     1141 tccttatccg ttctttgctg actttcccgg acaaagacct gaaggctttt ttataactga
     1201 aatcatatgt ttcttcagta tactgagtat tgccttgtcc cggaataaaa tcataagcac
     1261 tggctatagg taattcccgg taaatgctca atgacccttc ctggatagtg aatttaatat
     1321 ccggatcatt ggtgtactgc acaattccgt aacagccagg ttcgccggta aattcagtca
     1381 taactgtgct tgccggaacc tgacatgccg attcaacccg ggcaatgttt atttctgcgg
     1441 gtaaatcaat taaagccgat accagaaaaa ataaaaagta tttcatttaa gttaggtgtt
     1501 attaatttaa tgacacggca ttgagtatta aacaaaagta tcagtgtaaa aactggtgca
     1561 aatatgacag atatataaaa tttgtattcc tggggtcatg ataacaaaaa tgaaaaattc
     1621 aattaaacca aactacggag cattatcaat aatgaataat caatctgtaa tttatttgca
     1681 tttccatctc gattatcatt attatcataa catgatgcat tcaattttct gccccagaac
     1741 ggttcttttt ttccgcagcg ggcagaggag tgatccctgc ttcaaatgcc cgggataagg
     1801 gcagataagc atcatttccc attattgaca tattttgacc tggcaaactg tttttcaagt
     1861 tccgggagta tatcctgcgg gttggtcaat ggcatgtcct tttcatgaat ctcatatccc
     1921 agaagctgtg atttcttgaa cctttcaaaa acagggagct tcgacctgtt ggtggtttca
     1981 atatgatttc ccttctcatc gacgataata acctttggca gaaaaatacc cttccgtccg
     2041 atgaatttat ccatcaggct gacagcctct tcatattctt gccttgtagc tcttcccagt
     2101 gattcatacc ggtagatctt ttttccgtcc catgtggaaa ccagggcaaa gctttcggag
     2161 attttttcgt cgatgtgtat ttcctcaccg agagtatcgt gtcctgaggg catcagcagc
     2221 actttccctc cgccaccctc aatattggct gcataacgcg gcattgttat acctgaaatc
     2281 cagccctgca gatgctttat atagatttta ccgatctgca cgggagtgat aaaatgaact
     2341 attccttttt ccttgtgaca ttgcagcaga taataaggat gaatgccaat ccagaacatc
     2401 cttctgaatg tctctgccaa tgtttcataa taatcattta catgattcaa cagaacagtc
     2461 tggttgcgaa tagtgaatcc atgcccgtgt atttttcggg aaactgctgc caggctttct
     2521 gttatttcat gatagtggtt gatgtgcgtc atgaaataaa catatttcct gagccctgag
     2581 gtataccggt tggctgattc attgatcaga tccagcaatt catcagtaac tgccatcggc
     2641 agcacaacgg ggacccttgt ggcaatcctg atcacgcgaa gatgggggat cctgctaagt
     2701 tcttccaaaa cgtacctgag gcggcttttg cttatcataa gtgcatcccc accagtaacc
     2761 agtacttcgt tgatgttctt gttataaccg atgtaaaaca atcccttgtt tatttccatg
     2821 gtgctgacgg caaccgtgct atcaagagac ttagccctct gacagtgaac acaatatgac
     2881 gcacagctgg tattttcagc aacaaaaagg gcaatcctgt tgggatatct ctgatatgct
     2941 gccccgtaac ttcttgaact ctccgacatt gctccctctg cacctgtgtc aggaaaaagc
     3001 aggttggcag gtgtgggtac actttgccag aaaatcggat caagacgttt gttgggtttc
     3061 tctccttcag ccataaccgg atgaaacgga tcttcacaca tcagtgaggc atag
//
LOCUS       DAOOYG010000196         2997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_40125_length_2997_cov_7.974507, whole genome shotgun sequence.
ACCESSION   DAOOYG010000196 DAOOYG010000000
VERSION     DAOOYG010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40125_length_2997_cov_7.974507"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="PK288_11350"
     CDS             complement(<1..1755)
                     /locus_tag="PK288_11350"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPM19566.1"
                     /translation="MRIFKFLASPVFMGVLFIIFAVSLAVATFIENDYGSAAAYDSVY
                     DTRWFEFILLLLAINLIGMVIMQKLYRRSKITVFMFHMAFVVILAGAAITRYFGWEGS
                     MHIREGEEQSKCYSGDRYIGFELRDTSGRVVDAHSEKYSMSSHAPGRYREKLLINGLS
                     HELVLSRIIANATESVTDDPDGEPLISIVATDNSSREKMILRRGDIKVFGDVSIGFFP
                     AEDTRIKIMCDSSSFFIQSESPVSLVNMMTGESQAVGADVAAEIKPMQIIALENIKLV
                     VQKTAVKGSVKPVPADRGAENTGTNVFIFSLMSGNKTKATINVWDSGMPGMASGIVSM
                     GSETLEVFFGSREKILPFSIYLNDFVLERYPGSNSPSGYRSDVLLKDKTEGVERSFVI
                     FMNNVLKYRGFRFYQSSYDPDEQGTILSVNNDPAGMIVTYTGYGILFLFIILSLLNRK
                     SLFHSVNPGFWNSALRKRLPLVMMLIILTGTGMINGQKLVPDRRSAEEFGKILVQDQK
                     GRTEPLSALGNDILRKVARENKFEGLDPMQVYLGLYLDFDSWKDVPLIKVSNKELQGI
                     LGLGGSMAAFSDIVNIDGD"
     gene            complement(1792..2487)
                     /locus_tag="PK288_11355"
     CDS             complement(1792..2487)
                     /locus_tag="PK288_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPM19567.1"
                     /translation="MSEKEPLESGKANPLRWKNFQHLSKHELQLIDDNRYEASFRAGE
                     VILKQGSPSSSVLFLISGLAKIYTEADGHKNFLIEIAQPGALVMGPGAYINSRNTFSV
                     ASLVPSRVCFIDFNVFRHLAKTNGAFAESLLEDFCLKALTLQTKMVNLSRKKMPGRLA
                     EALLYLADEVFRSDEYELMLTRNELGEMTNMAKESVVRILKEMEESGVIYSRAPLIRI
                     LDRQKLILISEKG"
     gene            2695..>2997
                     /locus_tag="PK288_11360"
     CDS             2695..>2997
                     /locus_tag="PK288_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19568.1"
                     /translation="MKNLLRLVVTLFAAAFLFSACEGPMGPAGADGKDGADGKDANET
                     CKLCHNSTTVDAKVLEYSYSLHYKGDAYTIATMDNCVPCHSHQAFMHVVKNNTPATF"
BASE COUNT          838 a          747 c          580 g          832 t
ORIGIN      
        1 atccccgtca atattcacta tatcggaaaa agcagccatg cttccgccca gacccaggat
       61 gccctgcagc tccttgtttg acacctttat cagcggtaca tccttccagc tgtcaaaatc
      121 gaggtataac cccaggtaga cctgcatcgg atccaggcct tcaaatttat tttcccgggc
      181 cacctttctt aatatatcat tgccgagggc agacaggggt tccgttcttc ccttctgatc
      241 ctggacaaga attttcccga actcctctgc cgatctcctg tcaggaacaa gcttctggcc
      301 atttatcatg cctgtaccgg tcagaataat gagcatcata accaggggca atctttttct
      361 cagggccgag ttccagaaac ccggatttac actatgaaaa agcgatttcc tgtttaacag
      421 cgacaggatg ataaagagaa aaagaatacc gtatcccgta taagtcacaa tcatccctgc
      481 aggatcattg tttaccgaca gtattgtccc ctgctcgtca ggatcatagg acgactggta
      541 aaacctgaaa cccctgtatt taagcacatt attcataaat atcacgaagg atctttcaac
      601 accttcggtt ttgtccttca gcaatacatc gcttctgtaa ccggagggac tgtttgaccc
      661 gggatacctt tcaagcacaa aatcattgag ataaatgctg aagggcagga tcttttcacg
      721 tgagccgaaa aatacctcaa gggtttcgct ccccattgaa acgattccgg aagccatgcc
      781 tggcattccc gaatcccata catttattgt agcctttgtt ttatttcctg acatcaggct
      841 gaaaataaaa acattcgtgc ccgtattctc tgcccccctg tcggccggga caggtttaac
      901 agatccctta accgcagtct tctgaacaac cagctttatg ttctccagtg caataatctg
      961 catgggtttt atttcagcag caacatctgc gccgaccgcc tgcgattcac cggtcatcat
     1021 gttcacaagg ctgaccgggc tttctgactg aatgaaaaac gacgacgaat cgcacatgat
     1081 ttttatccgg gtgtcctcag ccggaaaaaa gccaattgaa acatccccga aaacctttat
     1141 atcgcctctc cggagaatca ttttttccct tgagctatta tctgttgcaa caatggaaat
     1201 gagtggttcg ccgtcaggat catcggttac tgattcagtg gcattggcaa tgatcctgga
     1261 aagaaccagc tcatggctca acccgtttat cagaagtttt tcacgatatc tgccgggagc
     1321 atgtgaagac atcgaatatt tttctgaatg tgcatccact accctgccgg atgtgtccct
     1381 taactcaaac ccgatatacc tgtcgcctga atagcacttg ctctgttctt ctccttccct
     1441 gatatgcatc gatccttccc atccgaaata cctggtaatt gctgccccgg caagaatgac
     1501 cacaaatgcc atatggaaca tgaagactgt aatttttgac cgcctgtaaa gcttctgcat
     1561 gataaccatt ccgatcaggt ttattgccag aagcagaagg atgaactcga accacctggt
     1621 atcataaacg gaatcgtagg cagctgcaga accgtaatca ttctcaatga aagtagcaac
     1681 agccagcgaa accgcaaaaa ttatgaataa cactcccata aatacaggag atgccagaaa
     1741 tttgaatatt ctcataaata attgtccctg ataattaaca ccaatcgatt ttcaaccctt
     1801 ttcggatatg aggatcagct tttgcctgtc aagtatcctg atcaatggcg cccttgagta
     1861 aataactccc gattcttcca tctctttcag gatccttaca acactttcct tggccatatt
     1921 tgtcatttct cccagctcat ttcttgtaag cataagttca tattcatccg agcggaacac
     1981 ctcatctgcc aaataaagca gggcttctgc gagccttccc ggcatttttt tcctggatag
     2041 gtttaccatt tttgtctgca gggtcaatgc ctttaaacag aaatcctcaa gaaggctttc
     2101 agcaaatgct ccgtttgttt tggccagatg acggaacaca ttgaaatcga tgaaacaaac
     2161 cctggacggg acaagcgatg caaccgaaaa agtatttctt gaattgatat aagctcccgg
     2221 tcccattaca agtgcacccg gctgggcaat ttcaatcagg aagttcttat gtccgtctgc
     2281 ttcagtgtaa atctttgcca gcccggaaat cagaaataaa acgctcgaag agggagaacc
     2341 ctgcttcagg atcacctccc ctgccctgaa cgatgcttca tatctgttat catcaataag
     2401 ctgcaactca tgttttgaca ggtgctggaa gtttttccac ctgagcggat tggcttttcc
     2461 tgattccaat ggttcttttt ccgacataac cgtgatatgc tttaaaactg atccggagct
     2521 tccttccagc cacaaatata acaattaaca aatattattg tttttattga tataaatcac
     2581 tatcttaata aatatttatc aactaaaaag atagtaaaaa gcatctgcct attaacgatt
     2641 cagaacttcc ttattgctac attgtggcct taaaaccaag aaaaacctga aaacatgaaa
     2701 aatcttctga gattggtcgt tacgttattt gccgctgcat ttttattttc cgcatgtgag
     2761 ggtccgatgg gcccggcagg tgctgacggg aaggatggtg ctgacgggaa ggatgccaat
     2821 gagacctgca agctttgtca caacagtaca acagttgatg ccaaagttct ggaatacagc
     2881 tattcactgc attataaagg agatgcttac acgatagcca caatggataa ttgtgttcca
     2941 tgccattcac accaggcatt catgcatgtg gtgaaaaaca atacccctgc cacgttt
//
LOCUS       DAOOYG010000197         2968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_40705_length_2968_cov_7.365602, whole genome shotgun sequence.
ACCESSION   DAOOYG010000197 DAOOYG010000000
VERSION     DAOOYG010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40705_length_2968_cov_7.365602"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PK288_11365"
     CDS             complement(<1..262)
                     /locus_tag="PK288_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19569.1"
                     /translation="MKKYLFLALVFILSSNCLNSQFIPPSFRPFTRQDTLRGSITPER
                     SWWDLLFYHLDISVNPADSSITGTNTVVYRVLRSSAVMQIDLQ"
     gene            complement(505..1353)
                     /locus_tag="PK288_11370"
     CDS             complement(505..1353)
                     /locus_tag="PK288_11370"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HPM19570.1"
                     /translation="MKKQTAALQFYVNVILIMVLCCSCHQSDIHSGFRVLSLHVGEHD
                     QAHISFVAEADRWFTEMAAEYGFRYVSSNNWNMLNHDTLSRYDVIIFLDSRPDSLPWR
                     EAFREYMENGGAWMGFHFAAFALTPSDYPQDWDWYHDLFLGSGQYVSNTWRPTPAVLR
                     VENHSCPATRNMPETFIASPNEWYRWEKDLRLNPDIDILVSIDSSSFPLGTGPKPHEI
                     WHSGYYPVVWTNRKYRMIYLNMGHNDIDYENRTNRELSFTFNNDTQNKLVIDALLWLG
                     NKKKTD"
     gene            complement(1474..2349)
                     /locus_tag="PK288_11375"
     CDS             complement(1474..2349)
                     /locus_tag="PK288_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPM19571.1"
                     /translation="MKNQPVFFMALFLGIFPGFAGGQNAKTGENPTASAIIESIIAKT
                     GSRVIPNTVDVIKAGDPETRITGIVTTMFATMDVLRKAVEMNCNLIIPHEPLFYNHLD
                     ETGSLKDDPVYLEKKKYIDDNKLVIWRFHDYIHSMQPDGILSGMANRLGWKDYIVNGR
                     LDDYSIPETTLEGMLGYLKKIFPGNAFYVVGNPDLRVSRVKFLCGSPGSSAHLKALAD
                     KSVDAVIAGEVPQWETYEYMRDAVLQGRPKAIVFIGHISSEESGMDFCADWLRSFIKG
                     IPVSFVASGSSYWSY"
     gene            complement(2524..>2968)
                     /locus_tag="PK288_11380"
     CDS             complement(2524..>2968)
                     /locus_tag="PK288_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPM19572.1"
                     /translation="LAGGPLFVAILMSRFGYKISLISYTTTSVNLMLREIGIVLFLAS
                     VGLNSGAKFVPALVSGEGFVWMGYGALITLLPLLLAGIFTRAVLKTNFLELCGLLSGS
                     MTDPPALAYANSIAQSEGPSIAYATVYPLTMFLRIFIAQILIVAF"
BASE COUNT          835 a          672 c          651 g          810 t
ORIGIN      
        1 cctgaagatc aatctgcatt accgcggagg agcgaaggac cctgtaaacc accgtgttgg
       61 tcccggttat ggaactgtca gccggattta cactgatatc aagatgatag aaaagaagat
      121 cccaccatga tctttcggga gttattgatc cgcgaagggt atcctgcctt gtaaaaggcc
      181 tgaatgacgg aggaatgaac tgggaattca gacaattgga tgataagatg aaaaccaaag
      241 ccaaaaagag atatttcttc attattccgg aaatcataaa ggctgatcct gccaaatgta
      301 taaaaaagag taaaacatgt gaagatccgg ggtttcttta ttgccatgtt cagtacacgc
      361 cggggcaata tttgcgtatg aaacgatctt aaattgccgg acagctttaa atgagcctct
      421 acggtgccgg ccttatagat accgtgtaaa cggaatcagc agggcaaaac aattattagc
      481 ggctcttaaa ccaggttgaa ataattaatc ggtttttttc ttattcccaa gccataagag
      541 cgcatcgatt acaagtttat tctgtgtgtc attattgaag gtaaatgaca gttccctgtt
      601 tgttctattc tcgtaatcaa tgtcattatg acccatattg aggtagatca tccggtattt
      661 ccggttcgtc cagaccacgg ggtagtatcc gctgtgccat atttcatggg gtttggggcc
      721 ggtgcccaaa ggaaagcttg aggagtctat cgacaccagg atatcaatat ccggattcag
      781 tctcagatct ttttcccacc ggtaccattc atttggagat gcaatgaatg tttccggcat
      841 gttccgggtt gccgggcagg aatggttttc gacacgcaat actgccgggg tcggccgcca
      901 ggtattactt acgtattggc ctgagcccag aaaaagatcg tgataccagt cccagtcctg
      961 gggataatcg ctgggcgtca gggcgaacgc tgcaaaatga aatcccatcc atgcccctcc
     1021 attttccata tattcccgga aagcttcccg ccagggaagc gagtctggcc tggaatcgag
     1081 aaaaatgata acatcgtatc gtgaaagggt gtcatgatta agcatgttcc agttgttgga
     1141 cgatacatac ctgaatccat attcagcagc catttccgtg aaccatctgt cagcttcggc
     1201 aacaaagctg atgtgagcct ggtcgtgctc cccgacatgg agtgacaaaa ccctgaaccc
     1261 cgaatgaata tctgactgat ggcaggaaca gcacaaaacc attatcagaa taacattcac
     1321 gtaaaattgc agtgcggcag tctgtttttt cattttcagt ttcaggcaag ggaaaaactt
     1381 ttttaaagaa tccaaacccg gcattcatcc atattgtcaa aaaatattcg taagccagcc
     1441 ggctgagtgc ctttatcctg tcattaatcc tacctaatat gaccaatacg aagatccgct
     1501 tgccacaaag ctgactggaa ttccttttat gaaactcctg agccagtcgg cacagaaatc
     1561 cattcctgat tcctctgaag aaatatgtcc gataaatact attgcctttg gccggccctg
     1621 aaggaccgca tcgcgcatat attcataagt ttcccattgg ggaacctcac cggcaattac
     1681 tgcatcaacg ctcttgtcgg ccaaagcctt taaatgggca ctgcttcccg gtgatccgca
     1741 caggaatttt acgcgtgaaa ccctgagatc ggggttccca acaacgtaaa atgcattccc
     1801 cggaaagatc ttcttgagat agccgagcat tccctcaaga gtggtttcgg gaattgaata
     1861 atcatcaagc cgcccgttca cgatataatc tttccatccg agcctgtttg ccatgccaga
     1921 taagatcccg tcaggctgca tgctatgaat atagtcgtga aatctccata tcaccagttt
     1981 attatcatct atatactttt tcttctcgag gtaaacagga tcatccttca gactacctgt
     2041 ctcgtcaaga tgattgtaaa aaagcggttc atggggaata atcaggttgc aattcatttc
     2101 caccgccttc ctgagaacat ccatggtggc gaacatggtg gtaacaattc ctgtgatcct
     2161 tgtttccgga tctcccgctt taattacatc gacagtattg gggatgaccc ggcttcctgt
     2221 ttttgcaatt attgattcaa tgattgcaga tgcagtggga ttttccccgg tttttgcatt
     2281 ctgtcctccg gcaaaaccgg gaaatattcc aaggaataaa gccataaaaa aaactggttg
     2341 atttttcatg tgtaaatata tttatatact actgaagatt aagtggttca cttggaaccc
     2401 acatgaattt tattttcatg cttggttgtg attttctatc ctgtgggatt catacttgaa
     2461 taattcttaa tttaattgtc agaaagcctg attcgtcaga ttatttaata aatatagaat
     2521 tttttaaaaa gcaactatca gtatctgggc aatgaaaatc cgcaggaaca ttgtaagcgg
     2581 atatactgtt gcataggcaa tggacggacc ttccgactgt gcgatgctgt ttgcatatgc
     2641 cagtgccgga ggatcggtca tactgccgga aagaaggccg catagctcaa gaaagttcgt
     2701 tttaagcact gcccgggtga aaataccggc taatagcagg ggtaacaaag tgatcaatgc
     2761 accgtaaccc atccacacaa atccctctcc tgataccaag gccggcacaa atttggcgcc
     2821 ggaattgagg cctacgctgg ccaggaaaag tacaatgcct atttcacgca acatcaggtt
     2881 tacacttgtt gtggtgtagg aaatcagtga aatcttgtac ccgaatctgc tcatcagtat
     2941 tgcaacgaac agcggtcccc cggcaagt
//
LOCUS       DAOOYG010000198         2909 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_41891_length_2909_cov_4.990189, whole genome shotgun sequence.
ACCESSION   DAOOYG010000198 DAOOYG010000000
VERSION     DAOOYG010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41891_length_2909_cov_4.990189"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /gene="rpoB"
                     /locus_tag="PK288_11385"
     CDS             <1..1453
                     /gene="rpoB"
                     /locus_tag="PK288_11385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152451.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPM19573.1"
                     /translation="KVVHEEMFTSVHIDEYLLEVRDTKRGLEELTSDIPNVSEEATKN
                     LDENGLIRIGAHILPGDILIGKITPKGESDPSPEEKLLRAIFGDKAGDVKDASLKAGP
                     SLEGVVIDKKLFTRAIKDRKAKTGEKPLLDKIEAEFNRSSHELKERLVDKLFILVNGK
                     TSQGVKDYLNVDVIPKGTKFTLKQLQEVDYLNINPNKWTTDKKKNDSIKQLLHNYIIK
                     FKEIDGIYKRKKYTITIGDELPAGIVRLAKVYIAKKRKIKVGDKMAGRHGNKGIVARI
                     VRQEDMPFLEDGTPVDIVQNPLGVPSRMNLGQIYETVLGWAGQKLGLQFSTPIFDGAT
                     IDQINEYTEKAGLPKYGKTYLYDGGTGERFDQPATVGVIYMMKLGHMVDDKMHARSIG
                     PYSLITQQPLGGKAQFGGQRFGEMEVWALEAFGAAHILQEILTIKSDDVIGRAKAYES
                     IVKGEPLPQPGIPESLNVLLHELRGLGLSVILD"
     gene            1502..>2909
                     /locus_tag="PK288_11390"
     CDS             1502..>2909
                     /locus_tag="PK288_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPM19574.1"
                     /translation="MSFRRDNKTKTSYSKISIGLASPEEILDRSSGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIS
                     LVVPIAHIWYFRSLPNKIGYLLGLPTKKLDSIIYYERYVVINPGIKAADGVKYLDFLT
                     EEEYLEITEFLPKENQYLDDNHPDKFVADMGAEALYKLLTRIDLDELSYSLRHRANTE
                     TSQQRKNEALKRLQVVEAFRDSRHVNKPEWMIIKVVPVIPPELRPLVPLDGGRFATSD
                     LNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKANAVKTDANR
                     PLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELDLHECGLPKDMAAELYKPF
                     IIRKLIERGIVKTVKSAKKIVDRKDPVVWDILENVLKGHPVLLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQLHPLVCTAY"
BASE COUNT          889 a          642 c          717 g          661 t
ORIGIN      
        1 gaaagtggtc catgaggaaa tgtttacttc ggtgcatatt gacgaatatc ttctcgaagt
       61 ccgtgacacc aagcgcggcc ttgaggagct gacatcagac ataccgaacg taagcgagga
      121 agcaacaaaa aacctcgacg aaaacggcct gatcaggatc ggagcccata tcctgccggg
      181 ggacattctt ataggaaaga tcactcccaa gggagaatct gatccttcac ccgaagagaa
      241 actcctgcgt gccatattcg gcgacaaggc cggagatgtc aaggacgcct cactgaaggc
      301 aggcccttca cttgagggag tggtaattga caagaaactg tttaccaggg caataaagga
      361 cagaaaagcc aagactggcg aaaaacccct gctcgacaag atagaagctg aattcaaccg
      421 cagctcccat gaattaaagg aaaggctggt ggataagctg ttcattcttg tgaacgggaa
      481 aacctcacag ggcgtaaagg attatctcaa cgttgacgtt attcccaaag gtaccaagtt
      541 taccctgaag cagcttcagg aagttgatta cctgaatatc aatcccaata aatggactac
      601 ggacaagaag aagaatgaca gcataaagca gcttcttcat aattacatca tcaagttcaa
      661 ggagattgac gggatttaca agcgcaaaaa atacaccatt accattggcg atgaactgcc
      721 tgcaggaata gtgaggcttg caaaggttta catagcaaag aagcgcaaga tcaaagtggg
      781 agacaagatg gcaggtcgtc atggcaacaa gggaattgtt gccaggatcg tacggcagga
      841 agatatgccg ttccttgaag acggcacgcc tgtcgacatc gtacaaaatc cgcttggagt
      901 cccttccagg atgaacctcg gccagattta tgaaacggtc cttggctggg ccggacagaa
      961 actaggcctt cagttttcaa ctccaatctt cgatggcgcc accatcgatc agattaatga
     1021 gtacactgag aaggccggat tgcccaaata tggcaaaacg tatttgtatg acggagggac
     1081 aggggaaagg tttgatcagc ctgcaactgt cggtgtgata tacatgatga aacttggaca
     1141 catggtcgac gacaagatgc atgcccgatc cattggtccc tactccctta ttacccagca
     1201 gccccttggc ggcaaggcac agttcggagg ccagaggttt ggtgaaatgg aagtctgggc
     1261 ccttgaagcc tttggagcag ctcacatcct tcaggaaata cttaccatta aatcagacga
     1321 tgttatcggt agggcaaaag cttatgaatc tattgtaaaa ggagaaccac tgcctcaacc
     1381 gggtattccg gagtcgctga acgtgctgct gcacgaactc agaggattgg gacttagtgt
     1441 tatacttgat taaacatcat ttatttactc attacaggtt tggattaaaa aaattcggta
     1501 tatgtcattc agaagagata acaaaaccaa aaccagttac tcgaaaatat caataggact
     1561 tgcttctccc gaagagatcc tcgacaggtc aagcggagaa gtgctgaaac ctgaaactat
     1621 caattaccgt acctataaac cggaacgcga tggccttttt tgcgaaagga tttttggacc
     1681 tgtcaaggat tacgaatgcc attgcggcaa atacaaaagg atccgctata agggtattgt
     1741 ttgtgacagg tgcggtgttg aagtaactga gaaaaaggtc cggcgcgaaa gaatgggaca
     1801 tatttcgctg gtggtaccaa ttgcacacat atggtatttc cggtcgctgc ccaataagat
     1861 cggttatctt ctgggccttc cgacaaagaa actcgattca ataatctatt acgaaaggta
     1921 cgttgtaata aacccgggca taaaggcagc cgacggagtt aaataccttg atttccttac
     1981 ggaagaagag tatcttgaaa taacggaatt ccttcccaag gaaaatcagt atcttgatga
     2041 caatcatcct gataagttcg tggcagatat gggtgcggaa gcattgtaca agctgcttac
     2101 ccggattgat ctagatgaat tgtcatattc acttcgtcac agggccaaca ctgaaacctc
     2161 acagcagaga aagaatgaag cactgaaaag gctccaggtc gtagaagcat tcagggattc
     2221 ccgacacgta aacaagccgg agtggatgat catcaaggtg gttcctgtca ttcctcctga
     2281 attgcgtcca ttggtacccc tcgacggagg gagattcgcc acaagcgatc tgaacgatct
     2341 ttacaggcgt gtcattattc gcaacaacag gctgaaaaga cttatcgaga ttaaagcacc
     2401 ggaagtgatc ctccgcaacg aaaaaagaat gcttcaggag gctgttgatt ctctttttga
     2461 caattcgcgg aaagcaaatg cggtaaaaac cgatgccaac cgtccgctga aatcgcttag
     2521 cgacagcctg aaaggcaaac aaggacgttt tcgccagaac ctgctgggaa agagggtgga
     2581 ttattcagca cgttcggtta tagttgttgg tccggagctg gatcttcatg aatgcggcct
     2641 tccgaaagat atggcggcag aactctacaa gccttttata atcaggaaac tgatcgagcg
     2701 cgggattgta aaaactgtta aatctgcaaa aaagattgtt gacagaaagg atccggttgt
     2761 atgggacata ctggaaaatg tacttaaggg tcaccccgta cttctgaacc gtgctccaac
     2821 cctgcacagg ctcggcatcc aggcgttcca gccaaagctt atcgaaggaa aggcaataca
     2881 gctgcatccc ctggtgtgta cggcataca
//
LOCUS       DAOOYG010000199         2865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_42815_length_2865_cov_7.026335, whole genome shotgun sequence.
ACCESSION   DAOOYG010000199 DAOOYG010000000
VERSION     DAOOYG010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42815_length_2865_cov_7.026335"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2279)
                     /locus_tag="PK288_11395"
     CDS             complement(<1..2279)
                     /locus_tag="PK288_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19575.1"
                     /translation="MRFINISFQKIRFAGLIILIAILAIPVSGQVQKEKRVRQMFNVT
                     LKVTDETGAPVPGASVVVGEGVTHLFTDANGSVSLSGYPEDVVTITAFKFEKKSGIVL
                     DLAANPTVILLQGKIHMTSGDIVPLPFTTLRKRNLTGSEVVVDGSYFSHYPSTDLRNS
                     LTGISSMYDVREIDGSPGLSPMEGLQQFSGLSNDYDATDKFNGMPYVIINDMPSELQE
                     MVIGPAEIESATLMKGILSTAMYGPAATGGVLYLKTKTGVPNERTLHFDVETGVSTVD
                     RMPGYVSGAEYARLVNRARYYEGLPAVYTGDEIKAFEKNDGYSLKNPSTDFADMILDN
                     TMEFRRVNMSSSGGNDVVQYYSYLGYAGEGDIIDLGAKSDYNRITARQNVNVKINDYF
                     SAQFGFYGNLTYRRSPNYGYDPDYTTEGTGNATLTLTELPSILDDLHTVPPVAYPIWA
                     YFDESSNTPWYGVSALYTNNLIGNVVDQGYYTDRGRTGASNLKLIYDAGNFVKGLKSE
                     TYFGFNIHNTVRVGKTNDYLAYTIKNPDGETLEDRITRYSGHSLFKMADNYKLMDYYF
                     QRWMFYERLSYDKTFANSNLQAAAMYHQTLSYINGIEEPQRQRSWLGTLNYNLMDKYQ
                     FQGVLNYAGNSSFAKDYRSMLNWAVGGAYVVTEDLITASNFLNYLKLRVQGGVVGNET
                     YFPNLYDVDRWSSTSTTSTSTPYAFGPYTSNRWFGTTTEAAVTRAYLSRTGNPILTWE
                     KRAEINGGFDAVLFNEKVTL"
     gene            complement(2305..>2865)
                     /locus_tag="PK288_11400"
     CDS             complement(2305..>2865)
                     /locus_tag="PK288_11400"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPM19576.1"
                     /translation="YNGRTYTGYALRKHWAGNSTKQQNIGGVWADALSRLSELYLNYA
                     EAVNEAYGPNGTAPGATLTALQAVNKIRTKSGMPDVRAEFTGSAAALRPRIKNERCIE
                     LSFEGQFYYDDVRRWMDLPQVMSSTLYAIIPQKTSDPEWPDGFKYLRTPLPADRQPAW
                     EEGMYYLYFLNADALKMKTFVANPVW"
BASE COUNT          757 a          657 c          601 g          850 t
ORIGIN      
        1 agtgttacct tttcattgaa cagtactgca tcaaatccac cgtttatttc cgctctcttt
       61 tcccaggtaa gaatcggatt acctgttctt gaaaggtaag ccctggttac ggcagcttcg
      121 gtggttgttc cgaaccagcg gtttgatgta tagggtccga aagcataagg ggttgatgta
      181 cttgtagttg aagtacttga ccatctgtcg acatcataaa ggttggggaa atatgtttcg
      241 ttgccgacaa ctccgccctg aacccgaagc ttcagataat taagaaagtt actggcggta
      301 atgagatcct ctgttacaac atatgctcct ccgactgccc agttaagcat gctgcggtaa
      361 tcttttgcga aacttgagtt acctgcataa ttgagaacac cctggaactg gtatttatcc
      421 ataaggttat aattaagtgt tcccagccag ctcctttgtc tttgaggctc ttctatcccg
      481 tttatgtatg aaagcgtctg gtgatacatc gcagcagcct gcaaattact gtttgcaaaa
      541 gttttgtcat agctcagcct ttcatagaac atccacctct ggaaataata atccataagc
      601 ttgtaattgt cggccatttt aaacagtgag tgtcctgaat acctggtaat gcgatcctcg
      661 agggtttctc cgtccggatt ttttattgtg taagccagat aatcgtttgt tttaccaaca
      721 cgcacagtat tatgaatgtt gaatccaaaa taggtttcgg atttaagacc cttaacaaaa
      781 ttgcctgcat cataaatcag tttcaggttt gatgccccgg tcctccctct gtcggtgtaa
      841 taaccctggt cgaccacatt tccgatcagg ttattggtat aaagtgcact cacaccatac
      901 catggagtat tgctcgattc atcaaaatat gcccagatcg gataagccac aggaggaacc
      961 gtatgaagat cgtccagtat tgaaggtaat tcggtaagtg taagtgtggc attacctgta
     1021 ccttctgttg tataatcagg atcatatccg tagttgggtg atcttctgta ggtaaggtta
     1081 ccgtaaaacc cgaactgtgc gctgaaataa tcattgatct ttacattgac attctgacgg
     1141 gcagtgatcc tgttatagtc ggattttgca cccagatcaa ttatatctcc ttcacctgca
     1201 taccccagat acgaataata ctggacaaca tcattccctc cggaggacga catattcacc
     1261 cttctgaatt ccattgtatt atccaggatc atgtcggcaa aatcggtact tggattttta
     1321 aggctgtagc cgtcattttt ttcaaatgcc tttatttcat caccggtata tacagccgga
     1381 agaccttcat agtatcttgc cctgttgaca agtcttgcat attcggcgcc ggaaacatat
     1441 cccggcattc tgtcaaccgt gcttactccg gtctcaacat caaagtgcag agtcctttca
     1501 ttcggtacgc ctgtcttggt ttttaaataa aggacacctc cggttgcggc cggtccgtac
     1561 attgctgtgg aaagaatacc tttcatcaaa gtggcagatt caatctcggc cggccctata
     1621 accatttcct gaagttcgga aggcatatca ttgataatca catagggcat cccgttgaat
     1681 ttgtcagttg catcataatc attcgacagt cccgagaatt gctgtagtcc ttccattgga
     1741 ctcaatccgg gggagccgtc aatttccctg acatcgtaca ttgaagatat ccctgtaagc
     1801 gaattgcgga gatccgttga agggtaatgt gaaaaataag atccgtcgac gacaacttcc
     1861 gaacctgtaa ggttacgttt cctcaaagtt gtgaacggca ggggaacaat atctcctgaa
     1921 gtcatgtgaa ttttgccctg caaaaggatt acagtcggat tggcagcaag atcaagtaca
     1981 atacctgatt tcttttcaaa cttaaaagca gttattgtaa caacatcttc aggataacca
     2041 cttaaggaaa cggaaccatt agcgtcggta aaaagatgtg taactccttc gccaacgaca
     2101 actgaagcac ccggtaccgg ggctccggtt tcgtccgtca ctttaagggt aacattaaac
     2161 atctgcctga ctcttttctc tttctgaacc tgaccgctca ccggtattgc cagtattgcg
     2221 ataaggataa tcagtccggc aaatctgatt ttttggaatg atatatttat aaatctcata
     2281 tacttgtgta ttatatggtt caatttacca gactggatta gctacgaaag ttttcatttt
     2341 cagagcatct gcattcagga agtacagata gtacattcct tcttcccatg caggctgacg
     2401 gtctgcagga agtggagtac ggagatattt gaatccgtcg ggccattcag gatcagaagt
     2461 cttctgcgga atgatcgcat atagcgtact tgacatcacc tgaggcagat ccatccatct
     2521 gcggacatcg tcataataga actgaccttc aaatgaaagc tcaatacaac gttcattttt
     2581 gatccttggt cttagtgctg cagcagatcc tgtaaattca gctctcacat ccggcattcc
     2641 cgatttagtt cttatcttgt tgacagcctg caatgcggtc aatgtcgcac ccggtgcagt
     2701 tccattagga ccataagctt cattgacagc ttcggcataa ttaagataaa gctctgaaag
     2761 gcgggacaga gcgtcggccc atacacctcc gatattctgc tgcttggtgc tgttcccagc
     2821 ccagtgcttt ctgagagcat atccggtata agtccttccg ttata
//
LOCUS       DAOOYG010000200         2831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_43568_length_2831_cov_7.728746, whole genome shotgun sequence.
ACCESSION   DAOOYG010000200 DAOOYG010000000
VERSION     DAOOYG010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43568_length_2831_cov_7.728746"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1151)
                     /locus_tag="PK288_11405"
     CDS             complement(150..1151)
                     /locus_tag="PK288_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPM19577.1"
                     /translation="MSNLFKVSLSPHIHGKETTQKLMFGVIVALIPAFLTSVFFFGYS
                     ALIVTATSVGSCILFEYLIVKFIFKKSITINDGSAIVTGLLLAFNLPSNIPVLIIVIG
                     SLVSIAVAKMTFGGLGNNPFNPALVGRVFMLISFPVQMTTWPVPAGFKTGYADAVTGA
                     TPLAIVKEGLKNGEPLSQLMTQIPTPAQMFLGNMGGSMGEVAAVALLLGLIWLLYKKI
                     ITWHIPVSIIGMIVVFTGILWLINPEEYASPLFHVLAGGVLLGAIFMATDYVTSPMNP
                     KAMLIYGCGIGLLTVIIRVWGAYPEGVSFAILIMNAFVPLMNAYIKPKRFGEEIKNG"
     gene            complement(1174..2505)
                     /gene="rsxC"
                     /locus_tag="PK288_11410"
     CDS             complement(1174..2505)
                     /gene="rsxC"
                     /locus_tag="PK288_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPM19578.1"
                     /translation="MKTFPVGGVHPPENKLTAEIPIRYLAIPPSVTIPVSQHIGEPAE
                     VVVNKGDIVKTGQIIARSKGFVSTNIHSSVSGTVNKIDLVLDSTGFRQTAVFIDTNGD
                     DWVDTIDRSDTIITEIKLSSEEIVKKCLESGIVGLGGATFPSHVKLTVPAGKRCDVLI
                     INGAECEPFLTSDHRLMLEKGKEILTGTTILMKALKVTKAFIGIENNKRDAVENLTKL
                     AAGFPGITVQALKVKYPQGAEKQLIKALTKREVPSGKLPLDVNAVVHNVGTAFAVYEA
                     VQKNKPLFERVVTITGKSVLNPGNFFVRTGTPVRYLIEAAGGLPENTGKIISGGPMMG
                     KAVSSIDVPVVKGTSGIVLIPEDQSERAEIMPCIRCAKCVLGCPMNLEPYLLMALAEK
                     GDFERAGNERITDCMECGSCSFTCPARRPLLDYIRLGKSTVIKMARKQIVK"
     gene            complement(2526..>2831)
                     /locus_tag="PK288_11415"
     CDS             complement(2526..>2831)
                     /locus_tag="PK288_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPM19579.1"
                     /translation="LRKKAKKDRKIYVACSNCDKGGPARRACKVACIACSKCVKVCKF
                     DAVTITNNLAYIDASKCTFCRKCVAECPTGCILEINFPPPKPAVEAKNETIKVNPEV"
BASE COUNT          737 a          658 c          604 g          832 t
ORIGIN      
        1 ctacctccct tattgcattg agcttcttat tcagcaccga cattgcaata ggctcttttg
       61 taagttcata aacaaaccca agccctgccg agctaacgag agcgattagc gtgagtgtca
      121 ggatcatgtt tttcagggtc gattcagatt tagccatttt ttatctcctc tccaaatctt
      181 tttggtttta tataagcatt catcagcggg acaaatgcat tcataataag tattgcaaaa
      241 gaaacacctt cggggtaagc tccccagact ctgattataa ctgtgagcaa accgataccg
      301 catccataga tcagcatagc ctttggattc atgggtgaag taacataatc ggtcgccatg
      361 aatattgcgc ccagaagaac cccgccggcc aggacatgaa acagcggact ggcatattct
      421 tcaggattaa tgagccacag gatgcctgta aacacgacta tcattccgat tattgaaaca
      481 ggtatgtgcc aggtaattat tttcttatac agaagccata tcaatcccag gagtagtgca
      541 actgcagcaa cttcacccat tgaacctccc atattaccaa ggaacatctg ggccggagtg
      601 gggatctggg tcataagctg tgacagtggt tccccgttct tgagtccctc ctttacaatt
      661 gcaagtggtg tggcacccgt cacagcatcg gcataaccag ttttaaaccc tgcaggtacc
      721 ggccaggtcg tcatctgaac agggaaagat atcagcatga aaacacggcc gaccagcgcc
      781 ggattgaaag ggttgttgcc cagacctccg aaggtcatct ttgctactgc aatcgatacc
      841 agacttccga tcacaattat taataccggg atgtttgatg gcaggttaaa tgcaaggagt
      901 aatccggtta caattgcaga accgtcatta attgttattg attttttaaa gatgaacttt
      961 acgatcagat actcgaacag tatacatgaa cccactgaag ttgctgtaac tatcagggcg
     1021 ctgtatccga aaaagaagac cgatgtaagg aatgccggaa taagggcaac aattacaccg
     1081 aacatcagtt tttgtgttgt ctctttcccg tggatatgag gtgacaggga tactttgaat
     1141 agattgctca tttgcaatag ttataatgac ttcttattta acaatctgtt tacgtgccat
     1201 ttttatgaca gttgattttc cgagccggat ataatccagc agagggcgtc tggcaggaca
     1261 tgtaaagctg cacgaaccgc attccatgca gtcagttatc cgttcattcc ccgctctttc
     1321 aaaatcacct ttttcagcca gtgccataag gagataaggt tccaggttca tcggacatcc
     1381 gagtacacat tttgcacatc ttatacatgg cattatttca gctctttccg actggtcttc
     1441 gggtataagc actatccctg aggttccttt tactacaggc acatcaatac tgcttactgc
     1501 tttacccatc atcgggccac ccgaaatgat tttacctgta ttttcaggca atcccccggc
     1561 tgcttcaata agatatctta ccggtgttcc tgttctgaca aagaaattgc caggattcag
     1621 gactgacttc ccggttattg tgaccaccct ttcgaaaagt ggtttgtttt tctggacagc
     1681 ctcataaaca gcaaaagctg ttcccacgtt atggacaaca gcatttacgt caaggggcag
     1741 tttcccggaa gggacttccc tttttgtaag agctttaatc agctgtttct cagctccctg
     1801 gggatacttc actttaagtg cctgaactgt tatcccggga aatcctgcag caagctttgt
     1861 aagattttca actgcatccc ttttattgtt ctcaatcccg atgaaagctt ttgtaacctt
     1921 taaagctttc ataagtattg tagttcctgt aaggatctcc ttcccttttt caagcataag
     1981 ccggtggtca gaggtaagga aaggttcaca ttcggcgccg ttgataataa ggacatcaca
     2041 tctcttaccg gcaggaacgg tgagcttcac atgtgaaggg aaggttgcgc cgccaagtcc
     2101 gactatgccc gattcaaggc atttttttac gatttcttca gatgatagtt ttatttcagt
     2161 tatgatggta tcgctcctgt caattgtatc aacccagtca tctccgtttg tatctatgaa
     2221 cactgctgtc tgcctgaaac ctgtggaatc cagaaccaga tctatcttat tgactgtacc
     2281 ggatacagat gagtgaatat ttgttgaaac aaaacccttg ctccgggcta taatctgtcc
     2341 ggtctttact atatctcctt tattcaccac cacctcggcc ggctcaccaa tgtgttgcga
     2401 aacggggatt gtcacggaag gaggtatggc caggtagcgg atcgggattt cggctgtcag
     2461 tttattttca ggcggatgaa cgcccccgac tggaaatgtt ttcaaatcag tatagtttat
     2521 gtttgttaga cttccgggtt gacttttatg gtttcattct tagcttcaac agcaggtttg
     2581 ggtggtggga aattaatctc aagtatgcat ccggtcgggc actcggcaac acatttcctg
     2641 cagaatgtac attttgaagc atcaatatag gcaaggttgt tggtgatggt gacagcatca
     2701 aatttgcata ccttaacgca tttcgaacag gctatgcatg caactttaca tgccctccgt
     2761 gccggtccac ctttatcgca gttcgaacag gccacatata tcttacggtc tttctttgct
     2821 ttcttcctga g
//
LOCUS       DAOOYG010000201         2808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_44077_length_2808_cov_6.259353, whole genome shotgun sequence.
ACCESSION   DAOOYG010000201 DAOOYG010000000
VERSION     DAOOYG010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44077_length_2808_cov_6.259353"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK288_11420"
     CDS             <1..430
                     /locus_tag="PK288_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HPM19580.1"
                     /translation="IGGEATLYFDNAYVGKSYLDANQLKDTMMISLGTDNSILVKREK
                     RQAYTSKKILGSNKTETYSFEISIRNNKPTTIRITVNDQIPVSSNSGIEVDAIELSKG
                     KYNRQTGEVKWDLEIKPQETRQLILTYSVKYPKDKTVILE"
     gene            554..928
                     /locus_tag="PK288_11425"
     CDS             554..928
                     /locus_tag="PK288_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HPM19581.1"
                     /translation="MKILGNIIWLISGGIIIVVEYFIGSLILMLTIIGIPFGLQTLKI
                     SSLLIWPFGRKSRIKERASGCLYIFMNVVWLLCGGIWIAATHAFFGILLCITIIGIPF
                     GMQHFKLTELALSPFGRDIERI"
     gene            1065..2102
                     /gene="thiL"
                     /locus_tag="PK288_11430"
     CDS             1065..2102
                     /gene="thiL"
                     /locus_tag="PK288_11430"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPM19582.1"
                     /translation="MNDPESRDTPIERLGKFGLIDRLCAGAGKINPGTVKGPGDDACI
                     IDTGNSLTLVSSDLLMEGIHFNLVYTPVKHLGYKAVIRGISDIFAMNGRPGQILVSLG
                     ISSRFSVEQIDSLYEGIYLACKRYNIDLAGGDTSSSLTGLTIGITSIGTVDRESITAR
                     DGAGPNDLVCVTGDLGAAYMGLQVLEREGRLFGMDKSFKPDLTGYEYIIERQLKPEFP
                     VDVLEQLREKEILPTSMIDISDGLASDILHICKSSGTGCRLYYEKIPIDTETFNTAEE
                     FGIDPVVAALNGGDDYELLFTSPPANYSRIMELKDVRIIGHLTAPEQGNMLISPEGTA
                     VELKAQGWNKL"
     gene            complement(2110..>2808)
                     /gene="lpxA"
                     /locus_tag="PK288_11435"
     CDS             complement(2110..>2808)
                     /gene="lpxA"
                     /locus_tag="PK288_11435"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPM19583.1"
                     /translation="ISGNVSIGDDSWIGPSATVFDGARIGRNCKIFPGAVISGIPQDM
                     KFKGEETTAELGDNTTVREGATVNRGTVAAGKTLVGSNCLIMINAHVGHDCRVGDYCL
                     LGNGTGLAGEVKVDDWAILSAGTLVHQFVRIGAHVMIGGGAKVRIDVPPYVKADRDPL
                     RYLGLNTVGLTRRGFEKERIDTIHNIFRALYQKGMNISKALEYIRENFDASPDRDYIL
                     EFIGKSERGVIRAG"
BASE COUNT          773 a          633 c          655 g          747 t
ORIGIN      
        1 tatagggggt gaagcaaccc tgtactttga caatgcctat gtcggcaaat catacctcga
       61 tgccaatcag ttgaaagaca caatgatgat ttcactcggc acggataaca gcattctggt
      121 aaaaagggag aagaggcagg catataccag taaaaaaatt ctgggctcaa ataaaacgga
      181 aacatattca tttgaaataa gcatcaggaa caataaaccg accacgataa ggatcaccgt
      241 taatgaccag atcccggttt cttccaacag cgggatagag gttgatgcaa tagagctttc
      301 aaagggaaag tataacaggc agacgggtga ggttaaatgg gaccttgaaa tcaagcccca
      361 ggaaacccgt cagctgatcc tcacctattc tgttaagtac ccgaaggata aaacagtgat
      421 ccttgaatga tcggaatctg gtccgcctcc tcatgacgtg ggaccggcag acaataagac
      481 aaatcgctga catgctgatt gcctggttgc cggaaggcac acaggtcagt ttaatattca
      541 atattgacta cccatgaaga ttcttggaaa catcatctgg cttatatcag gaggaataat
      601 catagttgtt gaatacttca tcggcagtct tatactgatg ctgaccataa tagggattcc
      661 cttcggcctg cagacactta agatctcatc tcttttaata tggccctttg gcaggaaaag
      721 ccggattaag gaaagggctt cgggatgtct ttatattttc atgaatgtag tctggctcct
      781 gtgcgggggt atctggattg cggcaacaca tgcttttttc ggcattttac tttgcatcac
      841 aataataggg attccgttcg gaatgcagca cttcaagctt actgaattgg ctctgagtcc
      901 gttcggacgt gacatcgaaa gaatctagaa aaccagggaa aagggtattg cgaataggaa
      961 aatgttattg ctgaaatggg tactttagct taatccggaa aaagatcatg atcctgatcg
     1021 caaatttgtc tggctggtca tacgcataca aaaaaccttg atgaatgaat gatcctgaaa
     1081 gcagagacac accgattgaa agacttggaa agtttggtct gatagaccgg ctttgtgctg
     1141 gtgcaggaaa aatcaatccc ggtacagtaa aaggcccggg tgatgatgca tgcataattg
     1201 acaccggtaa cagcctgacg ttagtatctt ctgatctgct aatggaggga atacatttca
     1261 acctggtata cacccctgta aaacatcttg gctataaggc tgtaataagg ggtatctccg
     1321 atattttcgc catgaatgga cgacctggcc agatcctggt atctcttggc atcagctcac
     1381 ggttctccgt tgagcagata gattcactct atgaaggaat ataccttgca tgcaaaagat
     1441 acaacattga tcttgccgga ggagatacat ccagctccct taccgggctc actattggta
     1501 ttacttcaat aggtacggtt gacagggaat ccataacagc ccgggacgga gcagggccga
     1561 atgatcttgt ttgcgttacg ggagatctgg gagctgcata tatgggcctg caggttcttg
     1621 aaagggaagg ccggttattt ggaatggata aaagtttcaa acccgatctg accggttatg
     1681 agtacattat cgaaaggcag cttaagccgg aatttcctgt tgatgttctt gaacagctca
     1741 gggaaaaaga aatccttcca acgtcaatga tagatatttc tgacgggctt gcttccgaca
     1801 tattgcatat ctgcaaatcg tcaggaaccg gatgtcgcct gtattatgaa aaaatcccaa
     1861 tagacactga aactttcaat acagccgaag aatttggtat cgatccggtt gttgccgctt
     1921 taaatggcgg ggatgattat gagcttcttt tcacctctcc gccggcaaac tattcaagga
     1981 ttatggaatt gaaagatgtg cggatcatcg gtcatctcac ggctcctgaa cagggaaata
     2041 tgctcatttc tcccgagggt accgcggtcg aactgaaggc acagggttgg aacaagctgt
     2101 gatgtactgc taacctgccc tgataactcc cctttccgac tttccgataa attcaaggat
     2161 gtaatccctg tcgggtgagg catcgaagtt ttccctgata tattcaagtg ctttggaaat
     2221 attcattcct ttctggtaga gtgccctgaa aatattgtgg attgtgtcga ttctttcctt
     2281 ttcgaatcct ctccttgtaa gacccactgt atttaaacca aggtatctga ggggatcacg
     2341 atcagctttg acatatggtg gcacatcaat ccttaccttt gccccgccgc caatcattac
     2401 atgagcgcca atccggacga actgatgtac cagcgtacct gcgctcagga ttgcccagtc
     2461 gtcaactttc acctctcctg ccaggcctgt gccgttaccc aggaggcaat aatcaccgac
     2521 cctgcagtcg tgtcctacgt gggcattgat cataatcagg caattactgc caacaagagt
     2581 ttttcctgcg gcaactgttc ccctgttcac cgtggcgcct tccctgacgg ttgtattgtc
     2641 gcccagttca gcagtggttt cctccccttt gaatttcatg tcctggggaa ttcccgagat
     2701 cactgcgccc ggaaagattt tacaattccg gccaattctt gcgccatcga aaactgttgc
     2761 actggggccg atccaggagt catctccaat tgatacattt ccggaaat
//
LOCUS       DAOOYG010000202         2716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_46239_length_2716_cov_5.182262, whole genome shotgun sequence.
ACCESSION   DAOOYG010000202 DAOOYG010000000
VERSION     DAOOYG010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46239_length_2716_cov_5.182262"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="PK288_11440"
     CDS             complement(<1..1449)
                     /locus_tag="PK288_11440"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HPM19584.1"
                     /translation="MTGPRSREAMDDPFSQVDGNAGAVRKYFWKKGLKNIFVGKMVCF
                     PDCAFGRKGMSIDFLWLRGCGKGLSAMVLESLEDQIEKFREDQKLLGKTRMISWKELE
                     EEEIIRLCDELTPRFDPDLYRSLLRLNLEESDRRRKEGLAILKGLDSNRRIMIQGPPG
                     SGKSTYAVDLIRRLTCDNAKTGLYICWNELLAAEMNQKLTDPELGIPPDRINVRLYFD
                     MASELGKLSGERSLIPSREVVKKGELRQCVKGAVSKAGRRPDKEKYDFVVVDEAQDIF
                     DKGIDFMLKALLKGNNPIENGSWFIFYDDSQDYPDGSDLTHYIRTRDMFSSYAATYNL
                     TSSLRVNTGHGIDEFVQDAASGILNIKKDYGEDVSISGWRSPEEAVKLIKSALVREKG
                     LGGVKNENCIVLFTADLLREGSELLSAIRQDEGFDLLAPGNYTIISGKARYTTMLKAK
                     GLERDVVILVSSPLSDRRNQFQVFIGASRAKAK"
     gene            complement(1501..1707)
                     /locus_tag="PK288_11445"
     CDS             complement(1501..1707)
                     /locus_tag="PK288_11445"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HPM19585.1"
                     /translation="MIKRLYSSWHIADQGERSADKLFTSLDDEIFYIIHHLRISSDYH
                     KTQLAGEIDFLVFTRLGLMVIEVK"
     gene            1930..>2716
                     /locus_tag="PK288_11450"
     CDS             1930..>2716
                     /locus_tag="PK288_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPM19586.1"
                     /translation="MKSRSIKNAIIGKSKSAAGRIVVLTGARQTGKTTLAKLAFPEMK
                     YIALDDPMLSGQYASLTADQWNHLYPSAVLDEVQKIPSVVESIKAVYDRFNDSRYILT
                     GSSQLLLMSKVRESLAGRCAIFELYPLTIPEILSQDWSDTVNRSFFQKYLESFELPPL
                     SPSFMMDAAYAERNAALRWYLEFGGYPALVDPALGNEERYEWLTGYVRTYLERDVRDL
                     ANFRYLEPFVKVQKMTSLLTGQLINYSDLAREAGVTAATAQRFV"
BASE COUNT          691 a          693 c          556 g          776 t
ORIGIN      
        1 cttcgccttt gccctcgacg cccctataaa tacctgaaac tgatttcttc tgtcagaaag
       61 cggtgagctg accagtatca caacatccct ctccaatccc tttgccttta gcatggttgt
      121 gtaacgtgcc ttgcctgata taattgtata attgcccgga gccaacaggt caaaaccttc
      181 atcttgccga attgcggaca acaattccga tccttccctg agaaggtcgg cagtaaaaag
      241 cacaatgcaa ttctcatttt tcactccccc aagtcctttc tcacgcacga gggcagattt
      301 tatcaactta actgcctcct cagggcttct ccatccgctt attgaaacat cctcgccgta
      361 atcctttttt atgttgagga ttcccgatgc tgcatcctga acaaattcat caatcccgtg
      421 ccctgtattg accctgaggc tcgatgtgag attatacgtt gcagcatatg agctgaacat
      481 gtcacgtgta cgtatgtaat gagtaagatc gctgccgtcg ggataatcct ggctgtcgtc
      541 gtagaagatg aaccagcttc cgttctcaat cggattgttg cccttcaaaa gggctttcag
      601 catgaagtcg atgcccttgt caaagatatc ctgggcttcg tcgactacca caaaatcgta
      661 tttctccttg tcgggccttc gtccggcttt tgaaaccgct cccttcacgc actgcctcag
      721 ctctcctttt tttacgacct ccctggaggg gatcagcgat ctttcgcccg ataattttcc
      781 aagttcggac gccatgtcaa aatagagtct cacatttatc ctgtcaggag gtatgccaag
      841 ttccggatcc gtcagctttt ggttcatctc cgctgccagg agctcgttcc agcagatata
      901 aagtccggtt ttggcattat cacaagttag tcgcctgatt aaatcgacag catatgttga
      961 ctttccactt ccggggggtc cttgtatcat tatgcgcctg ttggagtcaa ggccttttaa
     1021 tattgcaagc ccttcctttc tgcgcctgtc cgattcttcc aggttgagtc tcaaaaggga
     1081 tcggtacagg tcaggatcga acctgggcgt cagttcatcg cataatctga ttatttcctc
     1141 ctcttccagt tccttccagc ttatcatcct ggttttcccc aacagtttct gatcctccct
     1201 gaacttttcg atttggtctt ccagagattc aagaaccatt gctgacaggc ctttgccgca
     1261 tccccggagc cagagaaagt caatactcat tcccttccgg ccaaaggcac aatcagggaa
     1321 gcaaaccatt ttcccaacaa aaatattctt cagacctttt ttccaaaaat acttcctgac
     1381 tgcaccggca tttccatcaa cctgcgagaa aggatcgtcc atggcttccc ttgaacgggg
     1441 acctgtcatc ctgtaatacc ccgtcgtttc atctgacctt tggccgaaac caatctcacc
     1501 tcacttcact tcaatgacca tcagtccaag gcgggtaaaa acaagaaaat caatctcgcc
     1561 ggcaagttga gtcttatggt aatcagatga gatcctgaga tgatggatta tgtaaaatat
     1621 ctcatcatca aggctcgtga atagcttatc tgccgatcgt tcaccctggt cggcaatgtg
     1681 ccaggaactg taaaggcgct tgatcatact atgaaaattg ctcgtattga ttaaacggaa
     1741 attttatgga tttaatgcct gttttctgct attagttata caatgagctt aatagctgcc
     1801 gaccattcct ggttgtagac ttccacgatc caggtgagta aaataatcta aaagggtgga
     1861 ttttttattt gacttaatat taagaaaggc gtatatttat cactattaat atataaatta
     1921 cgccaaaata tgaaatcacg ttctataaaa aacgctatta tagggaagag taaatctgcc
     1981 gccgggagaa tagtcgtcct tacaggagca agacagactg gaaaaaccac ccttgcaaaa
     2041 ctcgcctttc ctgaaatgaa atacattgca cttgacgatc caatgctttc agggcaatat
     2101 gcatccctta cagctgacca gtggaaccat ttgtatccgt cggccgtcct cgatgaagta
     2161 cagaaaatcc cttctgttgt ggaaagcatc aaagccgtat atgacagatt caatgattcc
     2221 cgctatattc ttaccggttc aagccagctt ctcctgatga gcaaggtgcg tgaatcactg
     2281 gcaggccgat gcgcgatctt cgagctttat ccccttacaa tccctgaaat tttatctcaa
     2341 gactggtcag atactgtaaa cagatcattc ttccagaaat atcttgaatc tttcgagtta
     2401 ccgccgcttt caccttcatt tatgatggat gcagcatacg ctgaaagaaa tgccgcgctc
     2461 cggtggtatc ttgagttcgg aggatatccg gcactcgtcg atcctgcact gggcaatgaa
     2521 gagaggtatg aatggcttac cggctatgtc cgcacctatc ttgaacggga tgtgcgcgac
     2581 cttgctaatt tcaggtacct tgagcctttc gtcaaggtac agaagatgac atccctcctt
     2641 accgggcaac tgattaatta ttccgatctg gcaagggaag ccggagttac ggcggctact
     2701 gcacagagat ttgtaa
//
LOCUS       DAOOYG010000203         2648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_47944_length_2648_cov_5.335519, whole genome shotgun sequence.
ACCESSION   DAOOYG010000203 DAOOYG010000000
VERSION     DAOOYG010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47944_length_2648_cov_5.335519"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PK288_11455"
     CDS             complement(<1..985)
                     /locus_tag="PK288_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPM19587.1"
                     /translation="MRTRAVRIYGKRDLRLEEFELPPMQDDEILAQVISDSLCMSSYK
                     AAQQGADHKRVPDDIDIHPTIIGHEFSGIILEVGKKWQHKFSSGQKFSIQPAMAQTGS
                     LDAPGYSFRFIGGDATHIIIPDIVMESDCLLSYDGEAFFPASLSEPMSCIIGAFQANY
                     HISPGTYEHRMGIKKGGKMALLAGVGPMGLGAIDYVLHHERKPELLVVTDIDEQRLSR
                     AERLFPVEHAKTLGIKLSYINTGKMPDPVSHLRELSGNTGYDDVFVFAPVRSVIEQGD
                     AILGFDGCLNFFAGPTDPDFKAEFNFYNVHYASTHIVGTSGGNTDDMRESLD"
     gene            complement(994..>2648)
                     /locus_tag="PK288_11460"
     CDS             complement(994..>2648)
                     /locus_tag="PK288_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPM19588.1"
                     /translation="RYKFIVHLHPSIVNGVLCSRNAKHMISDLFGEEALFVPYTDPGY
                     TLFKKLESEIEKYRGRFHCDPKMIFLENHGVFAGSDSCDEIRIIYQTIISGISARVNP
                     VSEIRDLPLNPLVLKVLPAMRMLLTDEKTKVIRSRHNSLIAKYYVNQQEFHKISLPLI
                     PDIIVYCKTRYLYVEQSTTAERIIESLKTQLPRFKNEYGYLPGIIVIKDLGIFAVEDN
                     YKAAETALDVYEDLILISHYALQCGGIKFLTPEQTDFIDKWEVENYRRKAGLSQAGSN
                     RMENKIAVVTGGAQGFGAGIAEALFNLSVNIVIGDVNEEAGNDFVNNLNKSGTLKSKA
                     VFVKTDVSDPASVKELIDKTVSEFGGLDLMISNAGILKAGGLDEMDPDAFVKSTNVNY
                     NAYFYCAKYASEIMKIQNLEKPDYFADIVQINSKSGLRGSKKNFAYSGGKFGGIGLTQ
                     SFALELAPYRIKVNSICPGNFYEGPLWSDPQNGLFVQYLRTGKVPGAKSIEDVRRYYE
                     EQVPMKRGCRLEDVIKALLYVIDQEYETGQAIPVTGGQVMIN"
BASE COUNT          689 a          619 c          549 g          791 t
ORIGIN      
        1 ggtcgagcga ctcgcgcata tcatcagtat tgcctcctga agtgcccaca atatgagtag
       61 aagcataatg cacattatag aaattgaatt cagctttgaa atccggatcg gtaggcccgg
      121 caaaaaaatt aaggcaaccg tcgaatccaa gtatcgcatc accctgttct atgacagatc
      181 ttacaggtgc gaagacaaac acatcgtcat atcctgtatt tcctgaaagt tccctgaggt
      241 gacttacagg atcaggcatc ttacctgtgt ttatataaga aagctttatt cccagggtct
      301 ttgcatgttc aacaggaaag agacgttctg ccctggataa cctttgttcg tcaatatcgg
      361 taacgacaag cagctcaggt tttcgttcat ggtgaagcac ataatcgatt gcacccagac
      421 ccattggacc aacaccggcc agcagagcca ttttgcctcc ttttttgatt cccatcctgt
      481 gttcataagt tccgggagaa atatggtaat tagcctgaaa ggcgcctatg atacaagaca
      541 ttggttcgga gagtgatgcc gggaaaaatg cttccccgtc gtagctgagc agacaatcag
      601 attccatcac aatatccggg attattatat gcgtggcatc acctcctata aaacggaatg
      661 aatatccggg agcatcaagg gaacccgttt gcgccattgc cggctggatc gagaatttct
      721 ggccgctgct gaacttatgc tgccattttt tcccgacttc aagaattatt cctgagaact
      781 cgtgccctat tattgtaggg tggatgtcaa tatcgtcagg tacccgttta tggtcagcgc
      841 cctgctgggc agccttgtaa gatgacatgc acaggctgtc agaaattacc tgggccagta
      901 tttcatcatc ctgcattggc ggaagctcaa attcctcaag ccggagatcc cttttgccgt
      961 aaatcctgac tgccctggtt ctcattttta ttttcagttt atcattacct gtcccccggt
     1021 aaccgggata gcctgaccgg tctcatattc ctgatcgatc acatacaata atgcttttat
     1081 cacatcttca agcctgcagc ctcttttcat agggacctgc tcttcgtaat atcttctgac
     1141 atcttcaata cttttagctc cgggtacttt gcctgtcctt aaatactgga cgaaaagacc
     1201 attctgagga tcggaccaca ggggcccttc atagaagtta cccggacaga ttgaattgac
     1261 ctttatacgg tagggcgcca gttccagggc aaaagattgt gtcagtccta tccctccgaa
     1321 cttgccgcct gaatatgcaa aattcttttt gctgcctctc agtcctgatt ttgaattgat
     1381 ctgcacaata tcggcgaaat aatctggttt ctccaggttc tggatcttca tgatttccga
     1441 agcatatttg gcacagtaga aataagcgtt ataattgaca ttggtgcttt tcacgaaagc
     1501 atcaggatcc atttcatcaa ggcctccggc tttaaggata ccggcattgc ttatcataag
     1561 atccaatccg ccaaactcac tgacagtttt gtctatgagt tctttaaccg aggcgggatc
     1621 ggatacatcg gttttaacaa acactgcttt tgacttcaga gttccggatt tattaaggtt
     1681 gttcacgaaa tcatttcctg cttcttcatt tacgtcgcca ataacaatgt tcacactgag
     1741 attgaaaaga gcttctgcta tgccggcccc gaatccctgc gcccctcctg tcacaactgc
     1801 tattttgttc tccatcctgt tgctgcctgc ctgtgacagg cctgctttcc tcctgtaatt
     1861 ctcgacctcc catttatcaa tgaaatcagt ctgctcgggt gtcaggaatt taattccacc
     1921 gcattggagc gcataatgac ttatcaatat cagatcttca tagacatcga gagcggtctc
     1981 tgcagcttta taattatctt caacagcaaa tatcccaaga tctttaatta ctattatccc
     2041 tggcagatag ccgtattcat ttttaaacct tggcagttga gtcttcagcg attcgattat
     2101 cctttcggct gtagttgatt gttcaacata aaggtatctt gttttgcagt agacaataat
     2161 atcaggaata agaggaaggg agattttgtg aaactcctgc tgattgacat aatattttgc
     2221 aataagggaa ttatgacggg atcttatgac ctttgtcttt tcatcggtga ggagcattct
     2281 cattgccggc agtactttga ggaccaaggg attgagaggt aaatcccgga tttcacttac
     2341 cgggttgacc ctggcagaaa tacccgatat tatggtctgg taaataattc ttatttcatc
     2401 acaggaatct gaaccagcaa atactccatg gttttcgaga aagatcatct tcgggtcaca
     2461 gtgaaatctg ccacggtatt tttcgatttc actttcaagt ttcttgaaaa gggtgtaccc
     2521 cggatctgta tatggaacaa acagtgcttc ttcaccgaag agatcagaga tcatatgttt
     2581 tgcgtttctg ctgcatagca caccgttgac aattgacgga tgcagatgaa cgatgaattt
     2641 atacctta
//
LOCUS       DAOOYG010000204         2633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_48333_length_2633_cov_5.432118, whole genome shotgun sequence.
ACCESSION   DAOOYG010000204 DAOOYG010000000
VERSION     DAOOYG010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48333_length_2633_cov_5.432118"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PK288_11465"
     CDS             <1..791
                     /locus_tag="PK288_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19589.1"
                     /translation="VSSYMKKGSTYSDISVYLPTEDAWTAGRMPVEKQFIWAWGYYEM
                     RYVYFPSELEGFNPVWINSEFLDKASVADGNLHVGNARCKALYVDAEYLDIKVVKRLA
                     ELASRGLHIILKKEPREPGAISSPGYRELIDEIKRSENVMAQLPDDLIPFISGIQIPA
                     HWCRKDGDELYIFFPNPKARRLKFPLEYGQSLCEKNDTINVKIKYGGKEYKVDLIFEP
                     YQSLLYRIDKNNISEIDISFVPSEPSAKQRAPGYKAPWLVNQDF"
     gene            823..1908
                     /locus_tag="PK288_11470"
     CDS             823..1908
                     /locus_tag="PK288_11470"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPM19590.1"
                     /translation="MKKNNPERSGNDGQFGFTGRRTFLRKAATVAAGALVAPKVIGSG
                     KDPDGLPQKRYPDSSIVVLDKRFEKYFIWNTPVERLWTGALWGEGPVWFGDGRYLLFS
                     DIPNNRILRWNEDNGQVSVFRQPSNNSNGNTRDRQGRLITCEHDTQRITRTEYDGTIT
                     VLMDRYQGKPLNAPNDVVVHSNGSIWFTDPGYGIMSAYEGHLDDFELPTNVYRLDPST
                     GEATVVAGDFIKPNGLCFSPDEKLLYIIDTGQPSGKPQPIRVFDVVDGKRLTNGRLFC
                     DTGVKPSDGIRCDTDGNIWCTASGVGEGNDGVHVYAPDGKMIGKICLPEGCANLCFGG
                     AKRNRLFMIASQSLYSVYVNAHGALWP"
     gene            1951..2301
                     /locus_tag="PK288_11475"
     CDS             1951..2301
                     /locus_tag="PK288_11475"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPM19591.1"
                     /translation="MELAEKEKIENIIKSRKKANGFYSSKSDVFKSFLDMEQKTYRDG
                     SLRRLCKEMIAIGISVAINCESCMEWHIKQALDNGASEEQIIEAIEVGIEMAGGPGTV
                     SARFAMNVLEYYRK"
     gene            2343..>2633
                     /locus_tag="PK288_11480"
     CDS             2343..>2633
                     /locus_tag="PK288_11480"
                     /inference="COORDINATES: protein motif:HMM:NF039685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HPM19592.1"
                     /translation="MLDISDSELALLLAKVSGSERDSELGFFSKPFRLKFKEKELIIK
                     KYFPVRDDKTVNYIIRNHDSYVGELMSLGIRIPETVIKAVQSKRKHQIIIIQE"
BASE COUNT          798 a          525 c          629 g          681 t
ORIGIN      
        1 aagtttcgtc atatatgaag aaaggctcaa cttattcgga tatttccgtt tacctgccaa
       61 ctgaagatgc ctggactgcc gggagaatgc ctgtcgaaaa acaatttatc tgggcatggg
      121 gatattatga aatgcgctat gtttattttc cttctgaact ggaaggtttt aacccggtct
      181 ggatcaacag cgaattcctg gataaggctt cggtggctga tggcaatctt catgtgggta
      241 atgcccggtg caaagcattg tatgtcgatg cggaatatct cgacattaag gttgttaaac
      301 gtctggctga actggcctcc cggggattac acattatttt aaagaaggaa ccccgtgaac
      361 ccggagcaat ttcttcgccc ggttaccggg agttgataga tgagatcaaa agatcggaaa
      421 atgttatggc tcagttacct gatgatctta tcccgttcat ttcaggaata cagatccccg
      481 ctcactggtg caggaaggac ggtgatgaac tctatatctt tttcccgaat cccaaagccc
      541 gaaggctcaa attcccgctt gaatacgggc aatccctctg tgagaaaaat gatactatta
      601 atgttaaaat aaaatacgga gggaaagaat ataaagttga tcttattttc gaaccctatc
      661 agtcgctctt atacaggatt gataaaaaca atatcagtga aatagacata agcttcgttc
      721 catctgaacc ctcagcaaaa caaagagccc ctggatataa ggctccctgg ctcgtaaatc
      781 aagatttcta aatcaattct ggtaactaaa atattgctaa ctatgaagaa aaataaccct
      841 gaaagatccg gaaatgacgg tcaattcgga tttaccggac gccgtacctt tttaagaaag
      901 gcggcaactg ttgctgcagg tgcacttgtg gccccgaagg taattggatc cggtaaggat
      961 cctgacggac tgcctcagaa gagatatccc gattcttcaa tcgtggtcct tgacaagcgt
     1021 tttgaaaagt actttatctg gaataccccg gtagaaagac tatggacggg agcattgtgg
     1081 ggtgaaggac cagtttggtt cggcgacggc agatacttgt tattcagtga tatccctaat
     1141 aacaggatat tgaggtggaa tgaagataat ggtcaggtat cagtcttccg ccagccttca
     1201 aacaactcaa acgggaacac ccgggaccgc cagggaagac tgatcacttg tgaacacgat
     1261 actcagcgta ttacccgaac tgaatatgac gggaccatca ctgtattgat ggaccggtac
     1321 cagggtaagc ctctgaatgc tcccaatgat gtagtggtac attccaacgg ttctatctgg
     1381 tttactgatc cgggatatgg cataatgtcg gcctatgaag gtcacctgga tgatttcgag
     1441 cttcccacaa acgtctaccg tctggatcct tcaaccgggg aggctacggt agttgctggt
     1501 gattttatca aacctaacgg gttgtgcttt tcgcctgatg aaaaactgct gtatattatt
     1561 gacacgggac agccttcagg caaaccgcaa ccaataaggg tttttgatgt agttgatggc
     1621 aagaggctta caaacggaag gctgttctgc gatacaggtg taaagccatc cgacgggatc
     1681 cgctgcgaca ccgacggcaa tatatggtgc acagcaagcg gagtcgggga aggtaatgat
     1741 ggtgtacatg tctacgcccc ggatgggaaa atgatcggta aaatttgtct gcccgaagga
     1801 tgtgccaatc tgtgcttcgg cggcgcaaaa cggaaccgcc tgttcatgat cgcaagccag
     1861 tcactctact cggtctatgt aaatgctcat ggagcattat ggccatgatt aaacaaactg
     1921 atcctctaaa aaatatttaa tttttttaaa atggaactcg ccgaaaaaga aaaaatagag
     1981 aacattatta agtcaaggaa aaaagccaat ggattttaca gttcaaaatc cgatgttttt
     2041 aaatcgttcc ttgatatgga acaaaaaact taccgggatg gttcgctcag aaggttatgc
     2101 aaggaaatga tagcaatagg tatctctgtc gccattaact gcgaatcgtg catggaatgg
     2161 catattaaac aggcactcga taatggagcc tctgaagagc agatcattga agcaattgaa
     2221 gttggtatag agatggcggg aggaccaggc acagtgtcag caagatttgc catgaatgtg
     2281 cttgaatact acaggaaata agaccagcca gttgaatatt gtcatgatca aataattttt
     2341 ttatgcttga tatctcagat agtgaactgg cactgttgct ggcaaaggta agcggaagtg
     2401 aaagggattc tgaacttggt ttcttctcaa agccattccg cctgaaattc aaggaaaagg
     2461 aactcataat taaaaagtac tttccggtaa gagatgacaa gacagtaaat tatataatcc
     2521 ggaatcatga cagttacgtg ggagaattga tgtcgcttgg gataaggatt cctgaaacag
     2581 tcatcaaagc agttcagtca aagaggaagc atcagataat cataattcag gag
//
LOCUS       DAOOYG010000205         2590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_49504_length_2590_cov_5.751085, whole genome shotgun sequence.
ACCESSION   DAOOYG010000205 DAOOYG010000000
VERSION     DAOOYG010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49504_length_2590_cov_5.751085"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PK288_11485"
     CDS             <1..1107
                     /locus_tag="PK288_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HPM19593.1"
                     /translation="AALISMMEFPHVSADLIERTGMANGHVLAILLGLLIGTVSWAGS
                     MIAFGKLDGWIGDLRVKEMKYVNLTILAVMLGLIVFIMTREVEASRELMPFIYLLFGI
                     AVIYGVLFVMPIGGADMPVVISLLNSFTGVAAAMGGFLYNNQAMLTGGILVGSAGTIL
                     TVLMCKAMNRSLLNVIIGVFGGGGAAAGSTAGTVREISLSDAAVLLAYSKKIVIVPGY
                     GLAVAQAQHICHELDKLLDEKGVEVRYAIHPVAGRMPGHMNVLLAEADVPYEQLIESE
                     EINPDLPNTDVVMVIGANDVVNPAAEDDPSSPIYGMPIVRARDAKNIIVMKRGMGKGY
                     AAIENMLFFNPKTRMLFGDAKQTLQNLVSEIKNL"
     gene            1174..>2590
                     /locus_tag="PK288_11490"
     CDS             1174..>2590
                     /locus_tag="PK288_11490"
                     /inference="COORDINATES: protein motif:HMM:NF027515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HPM19594.1"
                     /translation="MPINKNLMDLNGNKLARLRKSLQISGTDIFILPCYDLHLGEYVP
                     GHWRIIEWLTGFTGSSATVVVTDSFAGLWTDSRYFIQAERQLSGTGFMTMKPELSSWT
                     GYQDFIRENVEPGMKIGVDGRIFPVRDFRKLKENLKDKDVFIDTECNPVDEIWSARPP
                     LPRAQAFDFPVEFAGKGRDEKISGVRKLMDQQGVDFHLLTSPDDIMWLLNIRGSDLKY
                     SPVLLSFALIGNDQILLFVDESGIPPKTASEFDTSGIVMLPYEETSEIISGVVRGYSV
                     LIDPRTTSVSIFNSLSGASRIMEDITFPARLKAVKNKTEIENIGRVMIRDGVALTRFF
                     HWLENNLGSSALTESSLSSMLEEFRSQQEGYRGPSFAPIIAFNENSALPHYDPSAGTA
                     AEIGDRGIILVDSGGQYAGGTTDITRTIAVGSPTQRQKTDFTLVLKGHINLALAKYPA
                     GTKGCQLDILARRALWESGLNY"
BASE COUNT          675 a          599 c          679 g          637 t
ORIGIN      
        1 gcggccctga tatccatgat ggaatttccg catgtctcag ctgacctcat tgagcgtacc
       61 ggtatggcaa atggtcacgt acttgcaatt ttgctgggct tgctgatcgg taccgtatcc
      121 tgggcgggaa gcatgattgc cttcgggaag ctcgacgggt ggatcggcga tttgagagtg
      181 aaggaaatga agtacgtcaa tcttacgatc cttgccgtca tgctgggatt gatagttttc
      241 atcatgacac gcgaggtgga ggcaagccgg gagctgatgc catttattta tctccttttc
      301 ggcattgcgg tcatctatgg cgtacttttc gtgatgccga tcggcggtgc cgacatgccg
      361 gtagtgatat cccttctaaa ttctttcacc ggggtagctg cagccatggg tgggttcctt
      421 tacaataacc aggcgatgct caccggaggt atactggttg gctccgcggg gactattctt
      481 actgttctga tgtgcaaggc aatgaacagg tcgcttctga acgtaataat aggcgttttc
      541 ggaggaggag gcgctgccgc cggctcaacg gcgggaacgg taagggaaat atccctgtct
      601 gatgccgcgg ttctcctggc gtactcaaaa aagatcgtta tcgttccggg ttacggactg
      661 gctgtagccc aagcccagca catatgccat gaacttgaca agctccttga cgaaaagggc
      721 gttgaggtga ggtatgcaat tcatcccgtc gccgggagaa tgcccggaca tatgaatgtc
      781 ctgctggcag aagcagatgt gccttatgaa cagcttatag aaagtgaaga aataaatccc
      841 gatcttccaa acaccgatgt tgtaatggtg atcggggcaa atgacgttgt aaacccggct
      901 gccgaggacg acccttcgag cccgatttac ggaatgccca ttgtcagggc ccgtgacgca
      961 aagaatataa tagtgatgaa acggggaatg ggcaagggtt atgctgcgat tgagaatatg
     1021 ctttttttca atcctaaaac gagaatgctg ttcggcgatg caaagcaaac ccttcagaac
     1081 cttgtcagtg aaatcaagaa cctgtgaact gattaacacc aaaaaagcat tcactcagga
     1141 atttgaccag agtcaaaccg gatatccggg cctgtgccca ttaataaaaa ccttatggac
     1201 cttaatggca ataaactggc acggcttcga aaatcgttgc agataagcgg tacggatatc
     1261 tttattctac cgtgttacga tctgcacctg ggtgaatatg tacccggcca ctggcggata
     1321 attgaatggc tgacaggctt tacaggatca tcggcaacag tggtggtcac tgattcattt
     1381 gccggactct ggaccgactc gagatatttt atccaggctg aaagacagct gtcaggtacc
     1441 gggttcatga cgatgaaacc ggaattatca tcctggacgg gataccagga tttcatccgc
     1501 gagaatgtcg aaccgggaat gaagatcgga gttgacggcc ggatatttcc tgtcagggat
     1561 ttcaggaaac tgaaggagaa ccttaaagac aaggatgtct tcattgacac cgaatgcaat
     1621 cctgtagatg agatctggtc tgcacgccca cccctgccgc gagcacaggc cttcgatttc
     1681 ccggttgaat ttgccggaaa gggaagggat gagaaaatat caggagtacg taaattaatg
     1741 gatcagcagg gtgtggactt ccaccttctt acttcccctg acgatataat gtggctcctc
     1801 aatatccgtg gcagcgactt gaaatacagt cctgtattgt tgtctttcgc attgatcgga
     1861 aacgatcaga tcctcctttt tgtagatgaa tcaggaattc ctccaaaaac agcatcggaa
     1921 tttgatacct ccggaatagt catgctccct tacgaggaga catctgaaat catttcaggc
     1981 gtcgtccggg gatattctgt gcttatcgat ccccggacaa cctccgtcag cattttcaat
     2041 tcattgtcag gcgcttcccg gataatggaa gatatcacat ttccggccag gctcaaggca
     2101 gtgaagaata agacagaaat agagaatatc ggccgggtga tgataagaga cggagttgca
     2161 ctcaccaggt ttttccattg gctggaaaac aacctgggca gctccgccct gacagaatcc
     2221 tcactatcat ccatgctgga ggagttcagg tcgcagcagg aaggttatcg cggaccttca
     2281 tttgccccga tcattgcttt caatgagaac agtgctcttc cgcattatga tccttcagca
     2341 ggcacagcgg ctgaaatagg agacaggggt attatccttg ttgattcagg aggccagtat
     2401 gccggaggaa caacggatat caccaggaca attgcagtcg gatcgcccac acaacggcaa
     2461 aaaactgact ttacgcttgt actgaaggga catattaacc tcgccctggc taaataccct
     2521 gcaggtacaa agggatgcca gcttgatatt cttgcacgaa gggcattatg ggaatccggt
     2581 ctgaattacg
//
LOCUS       DAOOYG010000206         2579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_49803_length_2579_cov_7.414422, whole genome shotgun sequence.
ACCESSION   DAOOYG010000206 DAOOYG010000000
VERSION     DAOOYG010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49803_length_2579_cov_7.414422"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PK288_11495"
     CDS             <1..601
                     /locus_tag="PK288_11495"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPM19595.1"
                     /translation="DAFRLRTQVPAPGKILTPPSAGRDIAGFRAEQYRLKTPDQPILD
                     RTILDAMEDYIPEIGKFEFRNEWSKPVLKGAIPAEPVAEREPVIVMKAPVRPYQSVRV
                     SPGIAESLIPIEKALPDGSATIALQVAIFHKESQALRAKKRIESRIDVTVEIVKQWDY
                     YHVLVTGFYTREETYQYYPELAGMGYPGITLIENYRRQK"
     gene            626..1186
                     /locus_tag="PK288_11500"
     CDS             626..1186
                     /locus_tag="PK288_11500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPM19596.1"
                     /translation="MKNIAIISPSVRTGRNSHRIALYLKNLIRENGLASVEILDLLRY
                     NFPLFNERLKYQDPPLKEAVDFAERIRTADGIVIVSPEYNGGSPASLKNAVDLLVEEW
                     NKKPVAFATVSDGMFAGTQAIISLQFSLSKLGAIIVPSVMRLPNIDKSFDENGIPADK
                     HKTDKRGIAFLKELLWFIEAKDRMAG"
     gene            1844..2431
                     /locus_tag="PK288_11505"
     CDS             1844..2431
                     /locus_tag="PK288_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19597.1"
                     /translation="MENISGFEYSRINTQMPGALTDPGDREKSKKFSNRVSARIVSEG
                     GNNLLEYINDQGIPAEGEIMVLSQDRHYYYDESDLKNIRILVNIKKLNLIERLDLFLD
                     TLFRILPPEADFIGCFTDSRMLKRDNGFTLYHSTKLLNQLISILDSKTNHYMNRDQVL
                     SLFAANGFKVYDMTEKDGLTYFHAKSIRKKAELRA"
BASE COUNT          761 a          583 c          589 g          646 t
ORIGIN      
        1 tgatgccttc cggctgcgga cacaggtacc cgcacccggt aaaatactta ctcctccgtc
       61 tgccgggcga gatattgccg gattcagggc tgagcagtac cggctgaaga cccctgatca
      121 gcctatcctt gaccgcacta tcctcgatgc aatggaggat tatattccgg agataggaaa
      181 atttgaattc aggaatgaat ggagcaagcc agtacttaag ggggcaatac cggccgaacc
      241 tgttgctgaa agggagcccg taattgttat gaaggccccg gtcaggccat atcagtctgt
      301 aagggtgagc ccgggaatcg ctgaaagtct cattcccatc gagaaagctt tgcctgacgg
      361 ttcggctaca attgcgctgc aggtggcaat cttccacaag gaatcacagg cattaagggc
      421 aaagaaacga attgaatcac gaattgatgt gacggtagaa atcgtgaagc aatgggacta
      481 ctaccacgtt ctggtaaccg ggttctatac ccgtgaagaa acctatcagt attatcccga
      541 acttgcgggc atgggttatc cggggatcac cctgattgag aattaccggc gtcagaaata
      601 agacctctct gttaattaat tcacaatgaa gaatattgca attatctcac caagtgtcag
      661 aacaggcagg aacagccaca ggatagcgct ttacctgaaa aatctgatca gggaaaatgg
      721 cttagcctca gttgagatac tggatcttct ccggtacaac tttccgttgt tcaatgaacg
      781 gctgaaatat caggatcctc cactgaagga agccgttgat ttcgccgaaa gaatccgtac
      841 agctgacgga attgtaattg tttctcccga atataacggt ggatctcctg ccagtctgaa
      901 aaacgctgtc gacctcctgg ttgaggaatg gaacaagaag cctgttgcct ttgcaactgt
      961 ttctgacggc atgtttgcag ggacacaggc aattatttca cttcagttca gcctttcaaa
     1021 gctgggtgca ataattgttc cttcagtcat gcgacttccg aacattgata aatcattcga
     1081 tgagaacggg attccggccg acaagcataa aactgataaa agaggcattg ctttcctgaa
     1141 ggaactttta tggttcattg aagcaaagga cagaatggcc ggctgaatta ccgtcgcccc
     1201 ggcatggcag aatatttccc ggcactggca gtcagggtca gattaatatc ctttcatccg
     1261 ctggcatacg aaacctgccg tgaaacccat acacctccaa atcctgaaat aattcccccg
     1321 ggtgcatttt tatagtaccg catcatttta taacctgcag tcatggatac tgtgcttttt
     1381 cccctcaatc gccaggggga aaaaacagac cggcaggaag cataaccaac cggcacgagg
     1441 gtgattaatc taatttctga cgttcaggga cggaaataat cctgagaaaa cgtattaaaa
     1501 acaggaaaga caggattttt aatcgaaaaa aatcatctgt ttgtcaaaat atattctttt
     1561 ctccggatta aagttattca tcactatctt gctcagcaac aaatacttaa ttaatctttt
     1621 atccggcctt gcctcctttc gcggaaaaac tggtaaattt taacctgatt ttgaccaacg
     1681 gccggcattc cggtacaagc atccggatac tttctgccag cgtcctgaac tgaatgacaa
     1741 gcggcccgca gaatgtgttc acattcccat ctaatgggca tctgcgtatt gttaaccatt
     1801 atcgacgcca ttatatttac catcatctta aaacatgaga gtgatggaaa atatttcagg
     1861 ttttgaatat tcccggatca acacccagat gcctggtgcc ttaacggatc cgggagacag
     1921 ggaaaagtca aaaaaattca gtaacagggt atctgccagg atagtatccg aaggcgggaa
     1981 taacctgttg gaatacatta atgaccaggg tatcccggct gagggggaga tcatggtcct
     2041 ttcccaggat cgccattact attatgatga gagcgatctg aaaaacatca ggatacttgt
     2101 aaatataaag aagctcaatc tgattgagcg ccttgatctg ttccttgata cgcttttccg
     2161 cattctgcct ccggaagcgg attttatagg atgtttcacc gacagcagga tgctaaaaag
     2221 ggacaatgga ttcacactgt atcattccac taagctcctc aatcagctaa taagtatcct
     2281 cgactcaaaa acgaatcatt acatgaacag ggaccaggtt ctgagtcttt ttgccgccaa
     2341 tggtttcaaa gtttatgaca tgaccgagaa ggacggtctc acttattttc atgcaaagag
     2401 tatacgcaaa aaggcagaac tgagggccta aaaatattga aagccggagt ggctgttaac
     2461 aagaattaaa accatagctc atgaaaacaa tttaccagat cacatcatgc gagacaggag
     2521 tggtgaccct gagaagaagg aaaattgcaa aggccctgcg ctggtggctg cgtgagaac
//
LOCUS       DAOOYG010000207         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_52948_length_2474_cov_7.852832, whole genome shotgun sequence.
ACCESSION   DAOOYG010000207 DAOOYG010000000
VERSION     DAOOYG010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52948_length_2474_cov_7.852832"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK288_11510"
     CDS             <1..212
                     /locus_tag="PK288_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPM19598.1"
                     /translation="ERLKCLYDGDEIEIGFKSVFLLEILANISSQDVMIELADPTRAG
                     LFLPAEAENTSEDLLMLLMPMMINA"
     gene            216..992
                     /locus_tag="PK288_11515"
     CDS             216..992
                     /locus_tag="PK288_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPM19599.1"
                     /translation="MELKLKRPLVFIDLETTGINVSADRIVEISALKVNPNGIEQWMT
                     TLVNPGIPIPPKVTAIHGISDSDVAEAPSFREIAKTLTSFLEGCDLAGFNAMKFDIPV
                     LAEEFLRANVDFNFRKRKYIDVQVIFHKKEQRTLEAAFRFYCKRELENAHSAKADTAA
                     TYEVLKAQLDTYTDLENDVDKLAEFSSFNNNVDFAGRIILDENGIETFNFGKHKGRRV
                     DEVFREEPSYYSWMMNGDFPLYTKKILTEIKLRAFGSKQT"
     gene            994..1605
                     /locus_tag="PK288_11520"
     CDS             994..1605
                     /locus_tag="PK288_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPM19600.1"
                     /translation="MKIICIGLNYRKHAIEMGRPFPAEPVVFLKPDSSILKKNKPFFL
                     PGFSSMIQYETEVVLKINRLGKSIPAKFAHRYYDEVTVGIDITARDIQNRNSAAGLPW
                     ELSKCFDGAAPLGTFIPVKSVRDPGSIDFRLEINGKTIQTGNTSDMIFSIDEIIEYVS
                     RFFTLKTGDLIFTGTPQGVGQLRKNDNLVAFLGDEQVLDFMIR"
     gene            1667..>2474
                     /locus_tag="PK288_11525"
     CDS             1667..>2474
                     /locus_tag="PK288_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008154480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM19601.1"
                     /translation="MKEKKKETRREFIKKSAGAAALLSMPTIIPSSVFGANGKVSVAV
                     IGVNGRGGSHIQGFMNLPNAEVTCLCDCDLNVLGPKAKEFETRYSKKVKQEQDLRNVF
                     ADKSIDAVSIATPNHWHALATIWACQAGKDVYVEKPGAHNIYEGRKMVEAANKYDRIV
                     QHGVQLRSSVAVQEAIKHLRDGLIGRVYMARGLVFRWRGDVGKKGPGTIPQGLDYDLW
                     TGPAEMKPFSEDWVHYNWHWHWNWGNGDVGNQGIHETDLCMWGLDVGLPEV"
BASE COUNT          739 a          497 c          589 g          649 t
ORIGIN      
        1 acgaaagatt aaaatgcctg tatgacggtg atgaaatcga aataggtttt aaatcagtgt
       61 tccttcttga aatacttgca aatatttcat cacaggatgt aatgatcgag cttgcggatc
      121 cgacgcgtgc cggattattt cttcctgccg aagctgaaaa tacatcagag gatctgctga
      181 tgcttctcat gccaatgatg ataaatgcct gatcattgga actgaagctt aaacgtccgc
      241 ttgtctttat tgacctcgaa acaacaggga taaatgtttc ggctgaccgt atagtcgaaa
      301 tttcagcact gaaggtcaat ccgaacggta ttgagcagtg gatgacaaca cttgtgaacc
      361 ccggaattcc cattcctccg aaggttacag caattcacgg gatctctgat tccgatgtag
      421 ctgaagctcc ttcattcagg gagattgcaa aaaccctgac atctttcctc gaaggatgcg
      481 atctggcagg tttcaatgcc atgaaatttg atatccccgt actcgcggag gaattcctga
      541 gagcaaacgt ggatttcaat ttccggaaga gaaaatatat tgatgtacag gtaatatttc
      601 ataagaagga gcaacgtaca ctcgaagccg cattcaggtt ctactgcaaa agggagcttg
      661 agaacgccca tagtgcgaaa gcagatactg cagcaactta tgaagtttta aaagcacagc
      721 tcgataccta tactgatctc gaaaatgatg ttgataagct cgctgaattc agttcgttca
      781 ataataacgt cgactttgcc ggaaggataa ttctcgatga aaacgggatt gagaccttca
      841 acttcgggaa gcataaagga agacgtgttg atgaagtttt cagggaagaa ccttcatatt
      901 attcgtggat gatgaacggt gattttcccc tttacacaaa gaagatactc actgagataa
      961 agctcagggc attcggcagc aaacagacat aggatgaaga tcatctgtat aggactcaat
     1021 tacagaaagc acgctattga gatgggcagg ccgttcccgg ccgagcctgt cgtcttcctt
     1081 aagcctgatt catcaatctt aaagaaaaac aaaccatttt ttcttccggg attttcttca
     1141 atgatccagt atgaaaccga agtagtattg aagataaacc gtcttggcaa aagcatccct
     1201 gcaaaatttg ctcacaggta ttatgatgaa gttacagtgg gaatagacat tactgcacgt
     1261 gatatacaga accgtaattc cgctgccggc ctcccctggg aattgtccaa atgttttgat
     1321 ggcgcggctc cgcttggtac attcatccct gttaaatctg tacgggatcc cggaagtatt
     1381 gacttcagac tggaaataaa cgggaagaca attcagacgg gaaatacttc agacatgata
     1441 ttcagcattg acgaaatcat tgagtatgtc tcccggttct ttacactgaa gacaggtgat
     1501 cttatattta caggcactcc tcagggagtg ggccaactga gaaaaaatga caacctcgtt
     1561 gcattccttg gagatgaaca ggtgcttgat tttatgataa ggtaaatatt ttttttatat
     1621 ttagccatcg ttaatttaca atccaaaact attcaataca actaacatga aagaaaagaa
     1681 aaaggaaacg agaagagagt ttattaaaaa atcggcagga gcggctgcac tcctttcgat
     1741 gcctacaatt attccttcaa gtgtgtttgg agcaaacggt aaggtatcgg tcgctgttat
     1801 tggtgttaac ggaaggggcg gaagtcatat ccagggattt atgaatcttc ctaatgccga
     1861 ggttacatgc ctctgcgatt gcgatctcaa tgtacttggc cccaaggcaa aagaatttga
     1921 gacacggtat tcgaagaagg taaaacagga gcaggacctg agaaatgtat ttgctgataa
     1981 atctatcgat gctgtaagta ttgctactcc gaaccactgg catgcactgg caactatctg
     2041 ggcctgccag gccggtaagg atgtttatgt cgagaaaccg ggagcccata atatttatga
     2101 aggccggaaa atggtcgaag cagcaaataa atacgaccgt attgtacagc atggcgttca
     2161 gctccgcagt tcagtagcag ttcaggaagc cataaagcac cttcgtgatg gtcttatcgg
     2221 ccgggtatat atggcaagag gacttgtctt ccgctggcgc ggtgatgtcg ggaaaaaagg
     2281 tccggggaca atacctcagg gactggatta tgatctctgg acaggaccgg cagagatgaa
     2341 gccattttcc gaagactggg ttcattataa ctggcactgg cattggaact ggggtaatgg
     2401 tgatgttggt aaccagggta ttcacgaaac tgatctttgc atgtggggac tggatgtagg
     2461 cttgcccgaa gtca
//
LOCUS       DAOOYG010000208         2348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_57104_length_2348_cov_7.582207, whole genome shotgun sequence.
ACCESSION   DAOOYG010000208 DAOOYG010000000
VERSION     DAOOYG010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57104_length_2348_cov_7.582207"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="PK288_11530"
     CDS             complement(<1..1583)
                     /locus_tag="PK288_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPM19602.1"
                     /translation="MIWNKFLSVGILLSLFSIINIYSQQAYLPPDKPRLVVGIVVEQL
                     RFDQLEKFRDRLSENGIRRLINEGTFFRNASFEYLLTQGAPGFSTISTGAEPAYHGIT
                     SDNWYQVLKDELVYCTKDISVDPVGGSYESGLHSPVNLQASTFTDELKLATNMQSKVY
                     AVGMKEYAAILSAGHAADGAFWFDNTSGTWMTSTYYMKSLPAWVNDFNSLKHPDTYLS
                     ETWNLSRPVQDYAICLPDTNNFEAGFEGKNYFPYDLKKLSQKKSGRSSRDYSLLRETP
                     FGNTLTTDFALRLIDSEELGQDEITDFLAICYSSTDYIGHRFGPSSVEAGDAIYRLDK
                     EVESVIRFLNDRLGKKNYLVYFTATHGVSEIPAVMEHNRIPAGYFRQNQALQLLRSYL
                     NAVYGEGNWVRGYSEKQVYLNRILIEDARIPLEEIQKKVARFMNQMTGVASAYPYSAF
                     ESNDYGGGHLKKIINNFSPQRSGDVIITLNPGWVEKDGEQITNHNSPYEYDSHVPLIW
                     YGWTVNRDVVMRRVNMTGIA"
     gene            complement(1683..>2348)
                     /locus_tag="PK288_11535"
     CDS             complement(1683..>2348)
                     /locus_tag="PK288_11535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPM19603.1"
                     /translation="YVTLELGGREVTIEIDDNFITPVYELQQLWDNNRQSLLNFIESA
                     LYGIHGLAVDAENGDPVPVRVIAERHEKDRSEVYADSITGRFIRLISPGTWNLVLSAD
                     GYRDTTVSDVRVIEYEKTDLLVRMKRIIDSIDTQEIRQPLMYPNPGGEFVKAVLPAGI
                     AGSINVQIFSAGGALLSDYNDYYTEGTPLILNINKLRRGIYIVRFTLKSNGTSYSARF
                     ILN"
BASE COUNT          613 a          579 c          496 g          660 t
ORIGIN      
        1 gctatgcccg tcatgtttac cctgcgcatt acaacatccc tgttaaccgt ccatccatac
       61 cagatgagcg gaacatgtga atcgtactcg tagggtgaat tgtgattggt tatctgctcc
      121 ccgtcttttt ccacccatcc gggattaagg gtaattatta catcgcccga acgctggggg
      181 ctgaaattat ttataatctt cttcagatgc ccccctccgt aatcgtttga ttcaaatgcc
      241 gaataaggat aggccgaagc aacacctgtc atctgattca taaaccttgc aaccttcttc
      301 tgaatttcct caagcggtat ccttgcatcc tcaataagta tcctgttcag atacacctgc
      361 ttttccgaat agccccgcac ccagttccct tccccgtaaa cggcattaag atagcttctt
      421 aacagctgaa gtgcctggtt ctgcctgaaa taccctgccg gaattcgatt gtgttccatt
      481 acagccggga tctcagaaac tccgtgagta gcagtaaaat atacaagata gttctttttc
      541 ccgagcctgt cattgagaaa cctgataaca gactccacct ccttgtcaag acggtaaatc
      601 gcatcaccgg cctcaaccga cgatggtccg aacctgtggc cgatataatc ggttgaggaa
      661 tagcaaatcg cgaggaaatc agtgatctca tcctggccaa gttcctcgga atcaatgagc
      721 ctaagggcaa aatccgttgt aagcgtattt ccgaacggcg tttcgcgaag aagggaatag
      781 tctctagagg atctccccga ttttttctga ctcagtttct tcagatcgta tggaaaatag
      841 tttttcccct caaatccggc ttcaaagttg ttcgtatcag gaagacagat ggcgtagtcc
      901 tgaacgggcc ttgacaggtt ccatgtttca ctcagatagg tatccggatg tttcagggaa
      961 ttgaaatcat taacccaggc cggaaggctt ttcatgtaat aagtggaggt catccaggtt
     1021 cctgaagtat tatcaaacca gaaagcgcca tcggcagcat gacctgccga gagaattgca
     1081 gcatattcct tcattcccac ggcataaacc tttgactgca tatttgttgc cagcttgagt
     1141 tcatccgtga atgttgaggc ctgaaggtta accggcgaat gcaatcccga ctcataactg
     1201 cccccaaccg gatcaacgga aatatctttt gtgcaataca caagttcatc cttaagtacc
     1261 tggtaccagt tgtcggatgt tattccatgg tatgccggtt cggccccggt agaaatagtc
     1321 gaaaacccgg gagctccctg cgtcagcaga tactcgaagg atgcattcct gaaaaatgtt
     1381 ccctcattaa tcagccttct tatgccattc tccgacaacc tgtccctgaa tttctcaagc
     1441 tggtcaaacc gcaattgctc aacgacaatc ccaactacca ggcggggttt gtcgggaggc
     1501 aggtatgcct gctgggaata tatgttaata attgaaaaca gactgaggag aatgccgacg
     1561 gacaaaaatt tgttccaaat catcgtttaa aggtgcggtt cggttatgaa atttttgaat
     1621 aaatgcaaaa gtagctcttt tcggatgaga aacaacaggc tgattttttt taacaatagc
     1681 tgtcagttca aaatgaatcg ggcggagtat gaagtgccat tacttttcag agtaaacctg
     1741 acgatgtaga ttcctcttcg cagtttgttg atattcagga ttaaaggagt tccttcggtg
     1801 taatagtcat tgtaatctga taataaggca ccgccggcac tgaatatttg tacattgatg
     1861 cttcctgcta tgcccgcagg aagtactgcc tttacaaatt ctcctccggg attcgggtac
     1921 atcaaaggct gacggatttc ctgagtatca atgctgtcaa ttattctttt cattctgacc
     1981 aggagatctg ttttttcata ttctatcacc cttacatctg aaaccgtggt atccctgtat
     2041 ccatcagcac tcaggacaag attccacgtt ccggggctga ttagtcttat aaaccggccc
     2101 gtgatactgt cagcataaac ctccgacctg tctttttcat gtctctccgc aattaccctg
     2161 accggcacgg gatcgccgtt ttcggcatcg acagccagcc catggatccc atacaatgca
     2221 ctttcaatga aattgagcag tgactgcctg ttgttgtccc aaagctgctg cagttcataa
     2281 acaggtgtaa tgaaattatc atctatctca attgtgactt cccgtcctcc cagttccagg
     2341 gttacata
//
LOCUS       DAOOYG010000209         2237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_61208_length_2237_cov_5.834555, whole genome shotgun sequence.
ACCESSION   DAOOYG010000209 DAOOYG010000000
VERSION     DAOOYG010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61208_length_2237_cov_5.834555"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /locus_tag="PK288_11540"
     CDS             <1..1861
                     /locus_tag="PK288_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPM19604.1"
                     /translation="VQFEKVKIIMGDTELCPYDEGTWGSLTVREFGPAMRIALAEARA
                     VLVEMASSQLGVPVSQLEVKDGIVYDIRNPSKKVSYAQLAKGKKLEKYLDVKPPVEDY
                     TKFTYVTKPFKRTDSVLKVTGQAKYTADLKLPGMVFARILRPPSHSAKLISVDVSGVE
                     QVPGTQVVRDGDFIAVLHSNKEKADEAIVKIKADYSINEPNVNDSNLYDRMVGADSTV
                     DIINSGGDLTEGSRLSSRLFESEFLDPYDAHASIETHTALGHMEGDRITVWASAQSPF
                     GLQDDIAAELGMPLEKVRVISPFVGGGFGGKIAHQQGVEVARLAKLAGKPVMLIWTRE
                     EEFFYDTFHPAGVYRVRSGMDKEGRIRMWDYGVYYGGTRGSDKIYDIPHFRITNYEQK
                     RGAPAIHPFLTGAWRAPNANSNTFARESQIDIMAAAAGIDPLEFRIKNLKDEKMIDCL
                     KAVADKFGYTPAKLPSGRGIGMACGTDAGTWVALMAEVRVDKDTGHVQVIRVSCAQDM
                     GLCVNPQGALLQIEGCVIMGMGYALSEEVKFNGGVIHTTGFDNFNIPRFSWVPKIDAV
                     ILDRKDKPPQGGGEPAIIAIGAIIANAIFDATGARMYRMPMTPERVLEALKKA"
     gene            complement(1995..>2237)
                     /locus_tag="PK288_11545"
     CDS             complement(1995..>2237)
                     /locus_tag="PK288_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19605.1"
                     /translation="YFGSYPDWRQPSAAFDNVDYLVKMLPFAGGMSYRNQPTDELSAK
                     MIRISKDSGYRGWYGIESSGREAIKKGIELLKKHLF"
BASE COUNT          608 a          487 c          603 g          539 t
ORIGIN      
        1 tgttcagttc gaaaaggtca aaataatcat gggtgacacg gaactttgcc cctatgatga
       61 aggaacatgg ggttcattga ccgtgaggga attcggtccg gcaatgagga ttgcactggc
      121 tgaggcaaga gcggtccttg tagaaatggc ttcgtctcag cttggtgttc ccgtttcaca
      181 actggaagtg aaggacggaa tcgtatatga tatccggaac ccgtcgaaaa aggtcagtta
      241 tgcccagctg gccaaaggga aaaaacttga gaaatacctc gatgtcaaac ctccggttga
      301 ggactataca aagtttacct atgttacaaa gccattcaag cgcacagact ccgttctgaa
      361 ggttaccggc caggcaaaat atacggcaga cttgaaactg cccggaatgg tttttgccag
      421 gattctcaga cccccttccc attcagcaaa actcatatcg gttgatgtct cgggtgttga
      481 acaggttccg ggaacacagg tagtgcgtga cggggatttt attgccgtcc ttcattcaaa
      541 caaggaaaag gcggatgagg caatagtaaa gataaaggca gattattcaa tcaacgaacc
      601 taatgttaat gacagtaacc tttatgaccg tatggtcggg gctgattcaa ccgtcgatat
      661 aataaattcc gggggtgatc ttaccgaggg cagcaggctg tcttccaggt tgtttgagtc
      721 tgaatttctc gatccatatg atgcacatgc atccattgag acacataccg ccctcgggca
      781 tatggaagga gacaggataa ccgtgtgggc ctcagcccaa agccccttcg gactccagga
      841 cgatattgcc gcggaactgg gcatgccgct tgaaaaggtc agggttatat cgccattcgt
      901 cggaggagga ttcgggggga agatagcgca ccagcaggga gttgaggttg caaggcttgc
      961 aaagctggcc ggaaagcctg taatgcttat ctggacgaga gaagaagagt ttttttatga
     1021 cacattccat cctgcgggag tttacagggt cagatcaggt atggacaagg agggcaggat
     1081 caggatgtgg gactatggag tatactacgg cggcacacgt ggatccgaca agatatacga
     1141 tatacctcat ttcaggataa caaattacga acagaaaagg ggagcaccgg ccattcaccc
     1201 gttccttacc ggtgcatgga gggccccgaa cgccaattca aacacttttg caagggaatc
     1261 gcagatagat attatggcag ctgctgccgg tattgatcct cttgagttcc ggataaaaaa
     1321 cctgaaggat gaaaaaatga ttgactgtct taaagcggtc gctgataaat tcggatatac
     1381 gcctgcaaag cttccatcgg gaagaggaat aggaatggct tgcggtactg atgccggaac
     1441 ctgggttgcc ctcatggccg aagtcagggt tgacaaggat accgggcatg ttcaggtaat
     1501 tcgggtcagt tgcgcacagg atatgggatt gtgcgttaac ccgcagggag cgctccttca
     1561 gattgaggga tgcgtaataa tgggaatggg ttatgccctt tctgaggagg ttaagttcaa
     1621 tggaggggta atacacacaa ccggatttga taatttcaat atcccaagat tctcatgggt
     1681 accgaaaatt gatgcagtga tccttgacag aaaagacaaa ccaccgcagg gcgggggcga
     1741 accggcaatt attgcaatag gtgccattat tgccaatgcg atatttgatg ccaccggggc
     1801 aaggatgtac agaatgccca tgacaccgga aagggtgctc gaagcgctta agaaagcctg
     1861 atatccgtcc tggtcacagg cggctgtacc ataggctgaa gttgttgtaa gcgaagcttt
     1921 tcagtaatca tttatgatac agccgccttc ttcattcgac tgcctgagat cccgtatcag
     1981 gttatcgcct gtaattaaaa aagatgcttc tttaaaagct caatgccttt tttaatagct
     2041 tcgcgtccgc tcgattctat gccgtaccat ccccggtaac cactgtcctt ggatattctt
     2101 atcatttttg cggacagttc gtccgttggc tggttccggt aggacattcc tcctgcaaag
     2161 ggaagcattt taaccaggta atcaacattg tcgaaggctg cggagggctg acgccagtcg
     2221 ggatagctgc cgaaata
//
LOCUS       DAOOYG010000210         2216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_62092_length_2216_cov_6.827857, whole genome shotgun sequence.
ACCESSION   DAOOYG010000210 DAOOYG010000000
VERSION     DAOOYG010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62092_length_2216_cov_6.827857"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PK288_11550"
     CDS             complement(<1..1181)
                     /locus_tag="PK288_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPM19606.1"
                     /translation="MKTIKITVGQAIVRFLKNQYVELDGQQNQFFAGCLGIFGHGIVA
                     GVGEGLLENPDFRYYMTRNEQASVHIATAYAKMKNRLGTFACISSIGPGATNMITGAA
                     CATINRLPVLIIPGDIFATRNVAPVLQQLESPSSQDISVNDCFKPVSKYWDRINRPEQ
                     LITSLPEVMRVLTSPADTGAVTLALPQDVQAEAFDFPQEMFRKRVWKIPRPRPDSEIL
                     KEAVKMIRASKKPVIIAGGGVIYSQATDALRKFVEKTGIPVAETFAGKGSLTFDHPLN
                     LGAAGATGTPGANKFASEADLIIGIGTRYSDFTTASKTAFGNPEVKFININVTCFDSH
                     KQGALPLTCDAKVCLEELSELLGNYSTAGEYRKAAADANRLWDEQVEGFFKVSGALPV
                     T"
     gene            complement(1296..>2216)
                     /locus_tag="PK288_11555"
     CDS             complement(1296..>2216)
                     /locus_tag="PK288_11555"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPM19607.1"
                     /translation="VLKQEWDLWKDRIPMIISENPSATEDEIAWRLIEEMSVKAAALL
                     KPVFDREKGKNGRLSIQTDPRLYRDAKRIVAQAERFSKLAPNMIVKIPVTKAGIEAIE
                     EATYRGISINATVSFTLPQAVKVAEAVERGLTRREKEGKEISTMGPVCTLMVGRLDDW
                     LKVVTEKENIIPNPDILDWAGVAVMKKAYKIYKERGYRLRLLSAAFRNHRHWSEFIGG
                     DVVISPPFIWQKRYNASDVTVENRMDKPVDPAIVSELERKYVDFRRAYYEDGMKAEEF
                     DRFGATLRTLRQFSQATTDVILIIRDMMLK"
BASE COUNT          543 a          527 c          461 g          685 t
ORIGIN      
        1 tgggttaccg gaagggctcc tgatacttta aagaaccctt caacctgttc atcccacaat
       61 ctgtttgcgt cggcagcagc ctttctatat tcaccggcag tggaataatt cccaagaagt
      121 tctgataatt cctcaaggca cacttttgcg tcacaggtga ggggtaaagc accctgtttg
      181 tgtgaatcga aacaggtcac attaatattt ataaatttta cctccggatt tccgaatgca
      241 gtttttgatg cagttgtaaa atcgctgtaa cgtgttccga taccgattat caggtctgcc
      301 tccgaagcaa acttattggc cccgggagtt cctgttgctc ctgctgctcc aagattcagg
      361 gggtgatcaa aggttaaaga accttttccc gcaaaggttt cagcgaccgg gatacctgtt
      421 ttttcaacaa attttctgag agcatccgta gcctgactgt aaattactcc gcctcctgca
      481 ataataactg gttttttcga agctcttatc atcttcacgg cttcctttag tatttctgaa
      541 tcgggccgcg gacggggtat tttccagact ctttttctga acatctcttg agggaagtcg
      601 aaagcctctg cctggacatc ctgtggaaga gccagagtaa cagccccggt atcggccggg
      661 gatgtaagta cgcgcatcac ttcaggcagt gatgtgatca attgttcggg cctgtttatc
      721 ctgtcccagt attttgatac cggtttgaaa cagtcattta ccgagatatc ctgtgatgag
      781 ggagattcga gctgttggag tacaggagcc acatttcttg tggcaaatat atccccgggg
      841 ataataagca caggaagccg gtttattgtt gcacaggctg caccggttat catgttagta
      901 gcccccgggc cgatcgagga gatacatgca aatgtcccga gacggttttt catttttgca
      961 tatgctgtag cgatatgcac cgatgcctgt tcattcctgg tcatatagta gcggaaatcc
     1021 gggttctcaa gtaatccttc cccgacaccg gcaacaatac catgaccaaa aatccccagg
     1081 caccctgcaa aaaattgatt ctgctggcca tcaagttcaa catattgatt tttaaggaat
     1141 ctgacaattg cctgaccgac tgttattttg atagttttca taatgtcatg tttatcagga
     1201 tactatttga attattatta agttaatcgg taatcagtaa tcggtaatca gtaatcagta
     1261 ttcagtattc agtaaccagc agccagcagg acaaactatt taagcatcat atcacgtatt
     1321 atcagaatga catcggtagt tgcctgactg aattgtctga gggttcgcag tgttgcacca
     1381 aacctgtcaa attcttcggc tttcatcccg tcttcgtaat atgcacgcct gaaatcaaca
     1441 tattttcttt cgagctctga tacaattgca ggatcaacag gtttatccat cctgttctca
     1501 actgttacat ccgatgcatt atatcttttc tgccagataa atggagggga aattacgaca
     1561 tccccgccga tgaattctga ccagtggcgg tggttcctga aagctgcgct taaaagtctc
     1621 agacggtatc ctctttcctt atagatctta tatgcttttt tcattacagc cacgcccgcc
     1681 cagtcaagta tatccggatt tggtattata ttttcctttt ccgtcacaac tttgagccag
     1741 tcatcaagtc ttccgaccat aagtgtacat actggtccca ttgttgaaat ttcctttcct
     1801 tctttttctc tccgtgttaa accccgctcc acagcttctg ccactttcac tgcctgtgga
     1861 agggtaaagc ttacggtggc atttatgctg atccctctgt aagttgcttc ttcaattgct
     1921 tcaatacctg ctttagtaac ggggatcttg acaatcatat tcggagcaag cttgctgaag
     1981 cgttcagcct gggcaacaat acgtttagca tccctgtata atcggggatc cgtttgtatt
     2041 gataatctgc cgttcttacc tttttcacgg tcaaataccg gtttgagcag ggcggcagcc
     2101 tttacagaca tttcttcaat cagtcgccag gcaatttcat cctctgttgc agatggattc
     2161 tcactgatta tcatcgggat cctgtctttc caaaggtccc attcctgctt taatac
//
LOCUS       DAOOYG010000211         2167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_64176_length_2167_cov_6.928504, whole genome shotgun sequence.
ACCESSION   DAOOYG010000211 DAOOYG010000000
VERSION     DAOOYG010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64176_length_2167_cov_6.928504"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK288_11560"
     CDS             <1..320
                     /locus_tag="PK288_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPM19608.1"
                     /translation="SSSLSLSRLFGILLVGSIRHADVLLVNGSINKRDRERLLELYNQ
                     APKPILVVAIGACGCTGGIFAEGITFAGPVDKIIPVDAYVPGCPPKPEAIIAGIVKLV
                     NKN"
     gene            394..846
                     /locus_tag="PK288_11565"
     CDS             394..846
                     /locus_tag="PK288_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPM19609.1"
                     /translation="MENHPAVQMLETELGSDFLKADIRTPKRIIVSIKPGAILRSAEY
                     LHKVAGFRFIIASALHTRNGFEIHYHFSRDQEGLILNLHVVLDAVEPRIESLSNMFSA
                     SNWIEREIHELFGIDFLNHPNQEKLISEGNWAEDVFPYRKKFKSATES"
     gene            849..2057
                     /locus_tag="PK288_11570"
     CDS             849..2057
                     /locus_tag="PK288_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPM19610.1"
                     /translation="MGRKVLIPIGPYHPLQEEPELFKLYCDGEIVRDVKWETGYNHRG
                     IEKLSESKTYEQVFFLVERICGICSTSHPFAFANAVEEIAGIEPTNRAKYIRSIIGEL
                     ERIHSHLLWLGLAGHFLGYNTVFMWAWKYREPILDLFEMITGNRNSYAMFRVGGVRRD
                     IEESKISAIEKVLDEFRRKNDLLTGAVMDDPVIHARTKGVGVLTRQDIIDYCAVGPTA
                     RASGVATDVRKDDPYAAYPLVDWQVVTAEEGDVFAKTKVRLLEMNESVSIIRQCLKGL
                     LKEKEGDIMVKVNEIPEGIGQGRAEAPRGEVFHFVVSDGSNSPARHKIRAPSYVNIAT
                     FKKSCIGQTISDATITLAAVDPCYCCTERLAVAYDFSTGEKIMNAKSLMDISVAKTAE
                     IMKIQGKKHE"
BASE COUNT          641 a          460 c          560 g          506 t
ORIGIN      
        1 gttccagcag cctttccctg tcacgcttgt ttggtatcct cctggtcggc agcatcaggc
       61 atgcggatgt cctcctggta aacggctcaa taaacaagcg tgacagggaa aggctgctgg
      121 aactgtacaa tcaggcacca aaaccaatac tggtggtggc aatcggtgca tgcggatgca
      181 ctggcgggat atttgccgaa gggataacct ttgccggacc agtcgacaaa atcatcccgg
      241 ttgacgcata tgttccggga tgcccgccaa agccggaggc cattattgca ggtattgtta
      301 aacttgttaa taaaaattaa tgacgataaa agagtttaag cagacaaaag aaaagggtca
      361 ataagtaaaa gacaaaatta tccggaagtt gaaatggaaa accatcctgc agtacaaatg
      421 cttgaaacag aattgggttc tgatttttta aaagccgaca tcagaactcc gaaacgcata
      481 attgtatcga taaaacccgg tgccatcctc agatcggcag aatatctgca caaagtagca
      541 ggattcaggt tcattattgc ttctgcactt cataccagga atggatttga gatacattac
      601 catttcagca gggatcagga aggattgatc cttaaccttc atgtcgtgct tgacgccgtg
      661 gaaccacgca tagaatcact ttcaaacatg ttcagtgcat caaactggat tgaaagggag
      721 atacatgagc tgttcgggat cgatttcctg aatcatccca accaggaaaa actgatctct
      781 gaaggcaatt gggccgaaga tgtgtttccg tatcgcaaaa aattcaaatc agcaaccgag
      841 tcataaacat gggcagaaaa gtactcatac cgattggtcc atatcatccg ctccaggagg
      901 aacctgaact gttcaagctg tattgcgacg gggagattgt cagggatgtc aaatgggaaa
      961 ccgggtataa ccacagggga attgaaaaac ttagtgaatc aaagacatac gagcaggtat
     1021 ttttcctggt tgagcgtata tgcgggatct gctcaacttc acatcctttc gcgtttgcca
     1081 atgcagttga agagattgcc ggtatcgaac caaccaaccg ggcaaaatat atcaggtcga
     1141 taatcggtga gcttgaacgt attcacagtc acctgttgtg gctgggtctt gcagggcatt
     1201 tcctcggcta caatacagta ttcatgtggg catggaaata ccgggagccc attctcgacc
     1261 tgtttgaaat gatcaccggc aacaggaaca gctatgccat gttcagggtc ggaggtgtga
     1321 gaagggatat tgaagagtca aagatatcgg ccattgagaa ggtcctggat gagttcagga
     1381 ggaagaatga cctgcttaca ggtgcagtaa tggatgatcc ggtgatccat gcacgaacaa
     1441 aaggcgtagg tgtgctcaca aggcaggata ttatcgacta ctgtgccgtg gggcccaccg
     1501 caagggcctc gggagtggca acagatgtga ggaaggatga tccctatgcc gcatatccgc
     1561 ttgttgactg gcaagttgta acggctgaag aaggagacgt ttttgccaaa acgaaggtcc
     1621 gtttactcga aatgaatgaa tccgtgtcaa taataaggca atgtctcaaa ggcttgctga
     1681 aggaaaagga aggcgacatc atggttaagg taaatgagat ccccgagggt ataggacagg
     1741 gaagggcaga agcccccagg ggagaagttt ttcactttgt ggtatcggat ggttcgaact
     1801 ccccggcgag acacaaaata agagctccga gctacgtgaa tattgcaacg ttcaaaaagt
     1861 cctgcatcgg acaaacaata tccgatgcaa ctataaccct ggcggcagtc gacccgtgct
     1921 actgctgcac cgagcgtctg gcagtggcct atgatttctc aacgggagaa aagatcatga
     1981 atgctaaatc cctgatggac atatcagtgg caaaaactgc agaaataatg aaaatccagg
     2041 gaaagaaaca tgaatgagtt tacaggcata gtatcggggt tttccgtctg ctgcatgctg
     2101 cagttactga tcggcattcc tgtcgctgca gggctgctgc tgttcctggt tccggaaaaa
     2161 tatctta
//
LOCUS       DAOOYG010000212         2140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_65373_length_2140_cov_6.355875, whole genome shotgun sequence.
ACCESSION   DAOOYG010000212 DAOOYG010000000
VERSION     DAOOYG010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65373_length_2140_cov_6.355875"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PK288_11575"
     CDS             <1..1137
                     /locus_tag="PK288_11575"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPM19611.1"
                     /translation="DRRYLDVMPGQIPVMGEKFPKPPVLYRDYRKLLENKGIDAVCIA
                     TPDHWHALQVIDAIDAGKDIYVEKPLTATIVEGRKMVEAQARTGRVVAVGLNRRGNVV
                     FQKLAKEIPAGKIGKVSVGRAARISNMAPDGIGKLKPENPPEGFNWDMWLGPRAFRPY
                     QYNIAPYMFRWWSDYSSQMGNWGVHYMDAIRWLMGETAPSVITAHGGKYVIDHDADIP
                     DTMQVTFEFASKKIITFSIYEATSGNFFPYGELELRGTKGNVYADERGYRIVPATKGQ
                     FQTWDKLIEEEEFKNPSPILDDGSSADSTSGLIRNFLDCIRTRQQPFCTLEEGHRSTS
                     FAHLANIALSTGERLCWDPVKEMFTNSEAANRLLHYQYRNPWKL"
     gene            1145..>2140
                     /locus_tag="PK288_11580"
     CDS             1145..>2140
                     /locus_tag="PK288_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HPM19612.1"
                     /translation="MMKQTRREFISRMTVAGAAVMANPFDFMTGGAAEPAPRTIRIKK
                     VSTNFEREPLIRPFGFKGGYMSEIWQSAARLESDSGAHAIGLCSQSVLWSDAEVFASH
                     SESGGNALMYAMTERAVQMMNGQSFTNPVDMLDSLYPEIYEYGKKITANPLLRKTFAL
                     NSLVAPDNAAWILYSRDNNIRTFDDMIPSRYKPALSHRHRRVAGIPLMAYTVPIEEIM
                     EAVDQGYFFMKIKIGQPGTQDEMLEKDKERLSAIHKAISHIRTPFSKDGKLPYYFDAN
                     GRYESKTTLLRLLDHARKIGAFDQIAIIEEPFPEEIEDDVSDIPVRLAADESAHTT"
BASE COUNT          601 a          483 c          564 g          492 t
ORIGIN      
        1 gacaggcgat atcttgatgt gatgcctgga cagataccgg tgatgggaga aaagttcccg
       61 aagcctccgg ttctgtacag ggattaccgt aaacttcttg aaaacaaagg aatagatgcg
      121 gtttgtattg caactcccga ccattggcat gccttgcagg taattgatgc aattgacgct
      181 ggcaaggaca tttatgttga aaagcctctt acagccacaa tagtagaagg aaggaagatg
      241 gtagaagccc aggcacgtac cgggcgcgtg gtagctgttg gcctgaaccg gaggggtaac
      301 gttgtattcc agaaactggc aaaagagatc cctgcaggaa agatcggaaa ggtttctgtg
      361 ggccgtgcag cacggatcag caatatggcc cccgacggga tcggtaaatt aaaacctgag
      421 aatccgcctg agggctttaa ctgggatatg tggctgggac cccgtgcttt cagaccatac
      481 cagtacaata ttgctccgta tatgttcaga tggtggagcg actattcaag ccagatggga
      541 aactggggag tgcattatat ggacgctata cggtggctga tgggtgagac tgctccatct
      601 gtgataacag cccacggtgg taaatatgta atcgaccatg atgcggatat ccctgacaca
      661 atgcaggtga cattcgaatt cgcatcaaaa aagataatta ctttctctat ttatgaggct
      721 acaagcggta atttcttccc ttacggcgag ctcgaactga gagggacaaa gggcaatgtt
      781 tatgccgacg aaaggggata caggattgtt cctgcaacca aagggcagtt tcagacctgg
      841 gacaaactga tcgaagagga agagttcaaa aatccaagtc cgatccttga tgacggcagc
      901 agcgctgaca gtacatcggg gcttatcagg aacttcctcg attgcatcag gacacggcaa
      961 cagccattct gcacccttga agaggggcac cgttcaacat cctttgccca tctggccaat
     1021 attgcccttt cgaccggcga aaggctgtgc tgggatccgg taaaggaaat gttcacaaac
     1081 agtgaagctg ccaacaggct tctgcattac cagtacagga atccctggaa actttagctt
     1141 tacaatgatg aaacaaacaa ggagggaatt catttcgagg atgactgttg ccggggctgc
     1201 tgttatggca aatccgtttg attttatgac aggcggggca gctgaaccgg cgcctcgtac
     1261 tatcaggata aaaaaggtca gcacgaattt tgaaagggag ccgcttatcc gtcccttcgg
     1321 attcaaggga ggatatatgt ctgaaatatg gcaatccgct gcacgacttg aaagtgattc
     1381 gggagcgcat gccatcggat tgtgttcaca aagtgtcctg tggtccgatg cagaggtgtt
     1441 cgccagtcac tccgagagcg gaggcaatgc cctgatgtat gccatgaccg aaagagccgt
     1501 tcagatgatg aacggtcaaa gcttcaccaa tccggtcgac atgctggatt ccctttatcc
     1561 tgagatctat gagtatggaa aaaagattac agcaaatccg ttgctgcgaa aaacttttgc
     1621 ccttaactca ctcgtcgccc ctgacaatgc cgcctggatc ctgtacagcc gggacaataa
     1681 catcaggaca tttgatgata tgatcccttc caggtataag cctgccctgt cgcacaggca
     1741 cagacgggtg gcgggaatac ctctgatggc ctacactgtt ccaatagagg aaatcatgga
     1801 ggcggttgat cagggttatt ttttcatgaa aattaaaata gggcagccag gaacacagga
     1861 tgaaatgctg gaaaaggata aagaacgcct gtccgcaatt cataaggcaa taagccatat
     1921 ccggacaccc ttctcgaaag acggaaaact gccttattat tttgatgcca atggccggta
     1981 cgagtcaaag actacactcc ttagactgct ggatcatgcc aggaagattg gcgccttcga
     2041 tcagattgca ataatagagg aaccttttcc tgaagagatc gaggatgatg taagtgatat
     2101 acctgtccgg ctcgctgccg atgaaagcgc ccatacgacc
//
LOCUS       DAOOYG010000213         1650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_3569
            NODE_96969_length_1650_cov_12.668339, whole genome shotgun
            sequence.
ACCESSION   DAOOYG010000213 DAOOYG010000000
VERSION     DAOOYG010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865304
            Sequence Read Archive: SRR5007225
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 17:29:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,317
            CDSs (total)                      :: 2,294
            Genes (coding)                    :: 2,285
            CDSs (with protein)               :: 2,285
            Genes (RNA)                       :: 23
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 19
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 6 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96969_length_1650_cov_12.668339"
                     /isolate="AMR_MDS_3569"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.12 N 87.63 W"
                     /collection_date="2015-01-07"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="PK288_11585"
     CDS             complement(<1..1599)
                     /locus_tag="PK288_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPM19613.1"
                     /translation="MKRKTFITLVSSAGGGVLLSSCSDRGTAGVPVKSGKKQISEHLS
                     ADLVIAGGGLGACAASIAACRNGLKVIMTEETDWIGGQVSQQGVPPDEHQWIETHGAP
                     ASYREFRKRVREYYKRNYPLIPEAAGREFLNPGDGSVSRLCHEPRVAVAVLNEMLAPY
                     VSSGKLILLTDHKIKSADIEGDRVRSLEAVSLITGNKIILSAPWFADATECGDLLPLT
                     VTEYITGTESKSETNELHAPEKADPENNQAFTVCFAMDYQPGIDNIKDPPADYDFWKN
                     YIPQLTPPWSGKLLDLKYSNPRTLEPKELGFHPEGIQTGDALNLWNYRRIINRNNFLP
                     GIYDGDITIVNWPQNDYFPGNIIDVSEKEFKKHVDAAKQLSLSLFYWLQTEAPRPDGK
                     QGWPGLRLRGDIMGTEDGMAKYPYIRESRRIKAVFTVLEEHVGAENRKLVAGDEAGKR
                     SAPFSDSVGIGYYHIDLHPSSQGNNYIDFPSLPFQIPLGALLPQRMNNLIPANKNIGT
                     THITNGCYRLHPVEWSIGEAAGCLV"
BASE COUNT          400 a          387 c          367 g          496 t
ORIGIN      
        1 aacaaggcaa ccggctgctt ccccgatgct ccattcaaca gggtgaagtc tgtaacaacc
       61 attagttata tgggttgtac ctatgttctt gttagccggg atcaggttat tcatcctttg
      121 aggtagcagg gcgccaaggg gtatctggaa tggaagtgaa gggaaatcaa tgtaattatt
      181 gccctgactg ctggggtgga ggtctatatg ataatagccg atcccgacac tgtcagaaaa
      241 aggagcagat ctttttcctg cttcatcgcc cgccacaagc tttctgtttt cggcgccgac
      301 atgttcttca aggactgtaa agacagcctt aattcttctt gattccctta tataaggata
      361 ctttgccatt ccgtcttcag ttcccattat gtcacctctt aatctgagcc ccggccatcc
      421 ctgttttcca tcaggacgcg gagcttcggt ctggagccag taaaacaacg acagactaag
      481 ctgctttgca gcatcgacat gcttcttaaa ttccttttca cttacatcga ttatgttacc
      541 tggaaaataa tcgttctgcg gccagttgac gatggttata tctccgtcgt atattcccgg
      601 caggaaattg ttcctgttga ttattctcct gtagttccac agattcaggg catctccggt
      661 ctgaatccct tccggatgga agccaagttc tttaggctcg agtgtcctgg gattggagta
      721 tttcagatcc aatagttttc ctgaccaggg aggggtcaac tgagggatat aattcttcca
      781 gaaatcataa tcagcaggtg gatcttttat attatcaata cctggctgat aatccattgc
      841 aaagcacact gtaaaagcct ggttgttctc cggatcggct ttttcaggag catgaagttc
      901 gttagtctcg ctttttgatt ccgtaccggt aatatattcc gttactgtaa gtggcagtaa
      961 atcaccgcat tcagtggcat ctgcaaacca gggagctgac aatattattt tgttcccggt
     1021 tatgagactg accgcttcga gggatctgac cctgtctcct tcaatgtccg cagattttat
     1081 tttatgatca gtcagaagga taagttttcc tgaacttaca tatggagcaa gcatttcatt
     1141 cagtactgca acagcaacac gcggttcatg acagagcctt gatactgaac catcgcccgg
     1201 attgaggaac tctctgcctg ctgcttccgg gattaagggg taattccgct tgtaatattc
     1261 cctcactctc ttcctgaatt ccctgtaaga agcgggggcg ccgtgtgttt caatccattg
     1321 atgttcatca ggcggtacgc cctgctgaga tacctgtcct ccgatccagt cggtttcttc
     1381 ggtcatgatc actttcaatc cattcctgca tgcggcaata gaggcagcac aggctccgag
     1441 gcctccgccg gcaataacta aatcagcgga gagatgttct gatatttgtt tttttcctga
     1501 cttgactggt actccggcag tgcccctgtc agaacaggac gacaataaga ctccaccgcc
     1561 tgccgatgaa acaagtgtta tgaaagtttt tcgtttcatt tcagagttat gttttataaa
     1621 ttgaaactgt tttaaaagta gataaaatat
//
